LOCUS       DBKELA010000001        78633 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.001, whole genome shotgun sequence.
ACCESSION   DBKELA010000001 DBKELA010000000
VERSION     DBKELA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.001"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..79
                     /locus_tag="ACUQR0_000001"
     CDS             <1..79
                     /locus_tag="ACUQR0_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107225.1"
                     /translation="INASYTQLEIKHSQPKNRASGKRVS"
     gene            497..1114
                     /locus_tag="ACUQR0_000002"
                     /pseudo
     CDS             497..1114
                     /locus_tag="ACUQR0_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1217..1540
                     /locus_tag="ACUQR0_000003"
     CDS             1217..1540
                     /locus_tag="ACUQR0_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HHV9107226.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1607..3064)
                     /gene="nuoN"
                     /locus_tag="ACUQR0_000004"
     CDS             complement(1607..3064)
                     /gene="nuoN"
                     /locus_tag="ACUQR0_000004"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HHV9107227.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3071..4600)
                     /gene="nuoM"
                     /locus_tag="ACUQR0_000005"
     CDS             complement(3071..4600)
                     /gene="nuoM"
                     /locus_tag="ACUQR0_000005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HHV9107228.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4764..6605)
                     /gene="nuoL"
                     /locus_tag="ACUQR0_000006"
     CDS             complement(4764..6605)
                     /gene="nuoL"
                     /locus_tag="ACUQR0_000006"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HHV9107229.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6602..6904)
                     /gene="nuoK"
                     /locus_tag="ACUQR0_000007"
     CDS             complement(6602..6904)
                     /gene="nuoK"
                     /locus_tag="ACUQR0_000007"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HHV9107230.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6901..7455)
                     /gene="nuoJ"
                     /locus_tag="ACUQR0_000008"
     CDS             complement(6901..7455)
                     /gene="nuoJ"
                     /locus_tag="ACUQR0_000008"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HHV9107231.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7467..8009)
                     /gene="nuoI"
                     /locus_tag="ACUQR0_000009"
     CDS             complement(7467..8009)
                     /gene="nuoI"
                     /locus_tag="ACUQR0_000009"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HHV9107232.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8024..9001)
                     /gene="nuoH"
                     /locus_tag="ACUQR0_000010"
     CDS             complement(8024..9001)
                     /gene="nuoH"
                     /locus_tag="ACUQR0_000010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HHV9107233.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8998..11724)
                     /gene="nuoG"
                     /locus_tag="ACUQR0_000011"
     CDS             complement(8998..11724)
                     /gene="nuoG"
                     /locus_tag="ACUQR0_000011"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HHV9107234.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11777..13114)
                     /gene="nuoF"
                     /locus_tag="ACUQR0_000012"
     CDS             complement(11777..13114)
                     /gene="nuoF"
                     /locus_tag="ACUQR0_000012"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HHV9107235.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13111..13611)
                     /gene="nuoE"
                     /locus_tag="ACUQR0_000013"
     CDS             complement(13111..13611)
                     /gene="nuoE"
                     /locus_tag="ACUQR0_000013"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HHV9107236.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13614..15416)
                     /gene="nuoC"
                     /locus_tag="ACUQR0_000014"
     CDS             complement(13614..15416)
                     /gene="nuoC"
                     /locus_tag="ACUQR0_000014"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HHV9107237.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15510..16172)
                     /gene="nuoB"
                     /locus_tag="ACUQR0_000015"
     CDS             complement(15510..16172)
                     /gene="nuoB"
                     /locus_tag="ACUQR0_000015"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HHV9107238.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16188..16631)
                     /gene="nuoA"
                     /locus_tag="ACUQR0_000016"
     CDS             complement(16188..16631)
                     /gene="nuoA"
                     /locus_tag="ACUQR0_000016"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HHV9107239.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17262..18200)
                     /gene="lrhA"
                     /locus_tag="ACUQR0_000017"
     CDS             complement(17262..18200)
                     /gene="lrhA"
                     /locus_tag="ACUQR0_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HHV9107240.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19120..20337
                     /gene="alaA"
                     /locus_tag="ACUQR0_000018"
     CDS             19120..20337
                     /gene="alaA"
                     /locus_tag="ACUQR0_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HHV9107241.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20421..21020
                     /gene="yfbR"
                     /locus_tag="ACUQR0_000019"
     CDS             20421..21020
                     /gene="yfbR"
                     /locus_tag="ACUQR0_000019"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HHV9107242.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21079..22911)
                     /locus_tag="ACUQR0_000020"
     CDS             complement(21079..22911)
                     /locus_tag="ACUQR0_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HHV9107243.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22998..23648)
                     /gene="hxpA"
                     /locus_tag="ACUQR0_000021"
     CDS             complement(22998..23648)
                     /gene="hxpA"
                     /locus_tag="ACUQR0_000021"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HHV9107244.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23659..24153)
                     /locus_tag="ACUQR0_000022"
     CDS             complement(23659..24153)
                     /locus_tag="ACUQR0_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HHV9107245.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24236..24691)
                     /gene="yfbV"
                     /locus_tag="ACUQR0_000023"
     CDS             complement(24236..24691)
                     /gene="yfbV"
                     /locus_tag="ACUQR0_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HHV9107246.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25029..26231
                     /gene="ackA"
                     /locus_tag="ACUQR0_000024"
     CDS             25029..26231
                     /gene="ackA"
                     /locus_tag="ACUQR0_000024"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HHV9107247.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26306..28450
                     /gene="pta"
                     /locus_tag="ACUQR0_000025"
     CDS             26306..28450
                     /gene="pta"
                     /locus_tag="ACUQR0_000025"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HHV9107248.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28640..30160
                     /gene="yfcC"
                     /locus_tag="ACUQR0_000026"
     CDS             28640..30160
                     /gene="yfcC"
                     /locus_tag="ACUQR0_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HHV9107249.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30193..30735)
                     /gene="yfcD"
                     /locus_tag="ACUQR0_000027"
     CDS             complement(30193..30735)
                     /gene="yfcD"
                     /locus_tag="ACUQR0_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HHV9107250.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30793..31344)
                     /gene="yfcE"
                     /locus_tag="ACUQR0_000028"
     CDS             complement(30793..31344)
                     /gene="yfcE"
                     /locus_tag="ACUQR0_000028"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HHV9107251.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31400..32044)
                     /gene="yfcF"
                     /locus_tag="ACUQR0_000029"
     CDS             complement(31400..32044)
                     /gene="yfcF"
                     /locus_tag="ACUQR0_000029"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HHV9107252.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32180..32827
                     /gene="yfcG"
                     /locus_tag="ACUQR0_000030"
     CDS             32180..32827
                     /gene="yfcG"
                     /locus_tag="ACUQR0_000030"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HHV9107253.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32884..33246
                     /gene="folX"
                     /locus_tag="ACUQR0_000031"
     CDS             32884..33246
                     /gene="folX"
                     /locus_tag="ACUQR0_000031"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HHV9107254.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33267..34160
                     /locus_tag="ACUQR0_000032"
     CDS             33267..34160
                     /locus_tag="ACUQR0_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HHV9107255.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34208..35098)
                     /gene="rpnB"
                     /locus_tag="ACUQR0_000033"
     CDS             complement(34208..35098)
                     /gene="rpnB"
                     /locus_tag="ACUQR0_000033"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HHV9107256.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35295..36068)
                     /gene="hisP"
                     /locus_tag="ACUQR0_000034"
     CDS             complement(35295..36068)
                     /gene="hisP"
                     /locus_tag="ACUQR0_000034"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HHV9107257.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36076..36792)
                     /locus_tag="ACUQR0_000035"
     CDS             complement(36076..36792)
                     /locus_tag="ACUQR0_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9107258.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36789..37475)
                     /gene="hisQ"
                     /locus_tag="ACUQR0_000036"
     CDS             complement(36789..37475)
                     /gene="hisQ"
                     /locus_tag="ACUQR0_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HHV9107259.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37565..38347)
                     /gene="hisJ"
                     /locus_tag="ACUQR0_000037"
     CDS             complement(37565..38347)
                     /gene="hisJ"
                     /locus_tag="ACUQR0_000037"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HHV9107260.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38568..39350)
                     /gene="argT"
                     /locus_tag="ACUQR0_000038"
     CDS             complement(38568..39350)
                     /gene="argT"
                     /locus_tag="ACUQR0_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HHV9107261.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39616..40185)
                     /gene="ubiX"
                     /locus_tag="ACUQR0_000039"
     CDS             complement(39616..40185)
                     /gene="ubiX"
                     /locus_tag="ACUQR0_000039"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HHV9107262.1"
                     /translation="MKRLIVGISGASGAIYGVRLLRVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40280..41797)
                     /gene="purF"
                     /locus_tag="ACUQR0_000040"
     CDS             complement(40280..41797)
                     /gene="purF"
                     /locus_tag="ACUQR0_000040"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HHV9107263.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41834..42322)
                     /gene="cvpA"
                     /locus_tag="ACUQR0_000041"
     CDS             complement(41834..42322)
                     /gene="cvpA"
                     /locus_tag="ACUQR0_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HHV9107264.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42581..43243)
                     /gene="dedD"
                     /locus_tag="ACUQR0_000042"
     CDS             complement(42581..43243)
                     /gene="dedD"
                     /locus_tag="ACUQR0_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HHV9107265.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43233..44501)
                     /gene="folC"
                     /locus_tag="ACUQR0_000043"
     CDS             complement(43233..44501)
                     /gene="folC"
                     /locus_tag="ACUQR0_000043"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HHV9107266.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44571..45485)
                     /gene="accD"
                     /locus_tag="ACUQR0_000044"
     CDS             complement(44571..45485)
                     /gene="accD"
                     /locus_tag="ACUQR0_000044"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HHV9107267.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45641..46300)
                     /locus_tag="ACUQR0_000045"
     CDS             complement(45641..46300)
                     /locus_tag="ACUQR0_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9107268.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46383..47195)
                     /gene="truA"
                     /locus_tag="ACUQR0_000046"
     CDS             complement(46383..47195)
                     /gene="truA"
                     /locus_tag="ACUQR0_000046"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HHV9107269.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47195..48208)
                     /locus_tag="ACUQR0_000047"
     CDS             complement(47195..48208)
                     /locus_tag="ACUQR0_000047"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HHV9107270.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48274..49410)
                     /gene="pdxB"
                     /locus_tag="ACUQR0_000048"
     CDS             complement(48274..49410)
                     /gene="pdxB"
                     /locus_tag="ACUQR0_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HHV9107271.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49509..50504
                     /gene="flk"
                     /locus_tag="ACUQR0_000049"
     CDS             49509..50504
                     /gene="flk"
                     /locus_tag="ACUQR0_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HHV9107272.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50501..51679)
                     /locus_tag="ACUQR0_000050"
     CDS             complement(50501..51679)
                     /locus_tag="ACUQR0_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9107273.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51934..53175)
                     /gene="fabB"
                     /locus_tag="ACUQR0_000051"
     CDS             complement(51934..53175)
                     /gene="fabB"
                     /locus_tag="ACUQR0_000051"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HHV9107274.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53334..55340
                     /gene="mnmC"
                     /locus_tag="ACUQR0_000052"
     CDS             53334..55340
                     /gene="mnmC"
                     /locus_tag="ACUQR0_000052"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HHV9107275.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55461..55739)
                     /locus_tag="ACUQR0_000053"
     CDS             complement(55461..55739)
                     /locus_tag="ACUQR0_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HHV9107276.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55773..56321)
                     /gene="epmC"
                     /locus_tag="ACUQR0_000054"
     CDS             complement(55773..56321)
                     /gene="epmC"
                     /locus_tag="ACUQR0_000054"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HHV9107277.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56321..57130)
                     /locus_tag="ACUQR0_000055"
     CDS             complement(56321..57130)
                     /locus_tag="ACUQR0_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HHV9107278.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57130..57954)
                     /gene="mepA"
                     /locus_tag="ACUQR0_000056"
     CDS             complement(57130..57954)
                     /gene="mepA"
                     /locus_tag="ACUQR0_000056"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HHV9107279.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57958..59043)
                     /gene="aroC"
                     /locus_tag="ACUQR0_000057"
     CDS             complement(57958..59043)
                     /gene="aroC"
                     /locus_tag="ACUQR0_000057"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HHV9107280.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59078..60010)
                     /gene="prmB"
                     /locus_tag="ACUQR0_000058"
     CDS             complement(59078..60010)
                     /gene="prmB"
                     /locus_tag="ACUQR0_000058"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHV9107281.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60176..60727
                     /gene="smrB"
                     /locus_tag="ACUQR0_000059"
     CDS             60176..60727
                     /gene="smrB"
                     /locus_tag="ACUQR0_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HHV9107282.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60831..61721)
                     /locus_tag="ACUQR0_000060"
     CDS             complement(60831..61721)
                     /locus_tag="ACUQR0_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HHV9107283.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61708..62232)
                     /locus_tag="ACUQR0_000061"
     CDS             complement(61708..62232)
                     /locus_tag="ACUQR0_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9107284.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62229..62699)
                     /locus_tag="ACUQR0_000062"
     CDS             complement(62229..62699)
                     /locus_tag="ACUQR0_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9107285.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62696..63244)
                     /locus_tag="ACUQR0_000063"
     CDS             complement(62696..63244)
                     /locus_tag="ACUQR0_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9107286.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63249..63971)
                     /locus_tag="ACUQR0_000064"
                     /pseudo
     CDS             complement(63249..63971)
                     /locus_tag="ACUQR0_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63991..66632)
                     /gene="yfcU"
                     /locus_tag="ACUQR0_000065"
                     /pseudo
     CDS             complement(63991..66632)
                     /gene="yfcU"
                     /locus_tag="ACUQR0_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66714..67277)
                     /locus_tag="ACUQR0_000066"
     CDS             complement(66714..67277)
                     /locus_tag="ACUQR0_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9107287.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(67961..68446)
                     /gene="sixA"
                     /locus_tag="ACUQR0_000067"
     CDS             complement(67961..68446)
                     /gene="sixA"
                     /locus_tag="ACUQR0_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HHV9107288.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68649..70793)
                     /gene="fadJ"
                     /locus_tag="ACUQR0_000068"
                     /pseudo
     CDS             complement(68649..70793)
                     /gene="fadJ"
                     /locus_tag="ACUQR0_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70793..72103)
                     /gene="fadI"
                     /locus_tag="ACUQR0_000069"
     CDS             complement(70793..72103)
                     /gene="fadI"
                     /locus_tag="ACUQR0_000069"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HHV9107289.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72283..72567)
                     /locus_tag="ACUQR0_000070"
     CDS             complement(72283..72567)
                     /locus_tag="ACUQR0_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HHV9107290.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72939..74279
                     /gene="fadL"
                     /locus_tag="ACUQR0_000071"
     CDS             72939..74279
                     /gene="fadL"
                     /locus_tag="ACUQR0_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HHV9107291.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            74645..75704
                     /gene="yfdF"
                     /locus_tag="ACUQR0_000072"
                     /pseudo
     CDS             74645..75704
                     /gene="yfdF"
                     /locus_tag="ACUQR0_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
     gene            complement(75886..76641)
                     /gene="mlaA"
                     /locus_tag="ACUQR0_000073"
     CDS             complement(75886..76641)
                     /gene="mlaA"
                     /locus_tag="ACUQR0_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HHV9107292.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            76935..77867
                     /locus_tag="ACUQR0_000074"
     CDS             76935..77867
                     /locus_tag="ACUQR0_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HHV9107293.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            77941..78015
                     /locus_tag="ACUQR0_000075"
     tRNA            77941..78015
                     /locus_tag="ACUQR0_000075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77974..77976,aa:Arg,seq:cct)
     gene            78177..>78633
                     /gene="intS"
                     /locus_tag="ACUQR0_000076"
     CDS             78177..>78633
                     /gene="intS"
                     /locus_tag="ACUQR0_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HHV9107294.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRG"
BASE COUNT        19909 a        21013 c        19298 g        18413 t
ORIGIN      
        1 aatcaatgct tcttatacac aattagaaat aaaacactca caacccaaaa atagagcatc
       61 cggcaaaaga gtgagttaac atcattgtta atcaattcga ccagtcagta aatatgctga
      121 ctggtcgaac ataatttgct aaaaagtcat cactgactga tgtcagtccc accatctgcc
      181 agttcaatct acgggtattt ccagcatatc gcggcacgcg attgctggtc tgttgatgta
      241 gtggattgta gttgctgact tttcttaccc tcagatttct gccccacttg tcaattcaat
      301 ctgccaataa ataacataat tatattttta ttcctcacag aaattccttt ttaaaaatag
      361 cgaacccact gtaaaaaaca ctttttgtat acctctacca agcgtaataa aataaacgct
      421 cataattcat tataaataat aatgaaataa cgccttttat tgataattta catatgaaaa
      481 aaattttcct tttgttatat tatttctgtt ttcactccct ctgattattt ttaccaaccc
      541 tgggtcactg cattatcgcc aacgccccga cacgtaagtc ctgagcaatt agaaaaaaca
      601 gttcgttatc ttacacaaac tgtgcatcca cgtaatgccg gcaatattga taatctgaat
      661 aggtccgcag agtatataaa agagttcttt gtcagtagcg gtgccagagt taccgcgcag
      721 ggcgtccccg attggctatt acgactctgc gcctggaagc cagaattacc cttacccggc
      781 aatgtcctgg ctttatcccg atcggggaga ctttattgct gtggtcggca gaatgcagga
      841 tatcaacgcc gttcgtcagg taaaagcggc attgttgtca tcccgggatt tatctgttta
      901 ttctataaat gccccagggt ttattcccgg tattgatttt tctgatcacc tcaattattg
      961 gcaacacgat attcccgccg taatgattac tgacaccgct ttttatcgta ataaacaata
     1021 ccacttgccc ggtgataccg cagacagatt gaattatcag aaaatggctc aggtggtaga
     1081 tggtgttata accttgttat acaacagtaa ataataaaaa tttctcgagg cgggtccaga
     1141 aacagcgaca tgtacatttt attttccctc gtctgtttta agatgcgaaa cataaaattc
     1201 aatggaggaa aaataaatgg gaatgattgg ctattttgca gaaattgact cagaaaagat
     1261 taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg acacgctttc
     1321 aggattacgc cgactggaat cactggatcg aagtgagctg cgtaaacagt tttccattaa
     1381 gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt tagaggggca
     1441 actctttgcc tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct
     1501 gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcaga gcgccaaaaa
     1561 aaaccgccga acatgtcggc ggctttctga cttacaaact aacagtttac atcagcggca
     1621 ttgctaaccg cacaatacta atcagcggtt gtggccatac acccagcacc agtaccaaca
     1681 gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgac ggtgcatcgc
     1741 gacccggttg ttccggtgcg tgcaggtaca ggctcaccgc cacgcgcagg tagtagtaga
     1801 ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt gcctggacac
     1861 cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata ccggccagag
     1921 acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt
     1981 aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc
     2041 cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc gcttccatcg
     2101 acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag
     2161 atgagtaacc gagcagacgt ttgatgttgg tctggctcaa cgccatcagg ttaccgaaga
     2221 tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg tcacccaccg
     2281 gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc gccgtcgcca
     2341 ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg
     2401 gcaccagaga gagtttgaag ccgaggccaa caatcatcat gccgaaacct gccagcaaca
     2461 gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc
     2521 cagactgcgc atacaccagc gccataccga acagcaggaa agaagacgct gcggcagaaa
     2581 ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca
     2641 ggccaaacag cggcaaagag atcagttcaa taccgaggaa cagagacgcc agatggtttg
     2701 cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag aactcatcct
     2761 tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg aggctcgcca
     2821 acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt
     2881 ccatagcgcc cgcctggcca acaaaccaga gcgaaaccag cgccgcgtta agcccaataa
     2941 ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca
     3001 ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg
     3061 tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc
     3121 caatcgcgga gtgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca
     3181 gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc agttcctggc
     3241 tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa
     3301 cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca acctggaagc
     3361 tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg
     3421 ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca
     3481 tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accgcagaga ataaacagac
     3541 ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc
     3601 tgccggtgta gattgcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca
     3661 gacgtttgat atcggtctga gcgaaggtca tccacgcacc gtagaagatg ccgataacac
     3721 ccagccacat agcgattggc gcgaactctg ccgacgcgtt cgggaacagc ggcagggaga
     3781 aacgcaacaa accgtaagcg gcggttttca gcaagatccc cgcgaggtcc acggaaccgg
     3841 cggtcggagc ctgggagtgc gcatccggca gccagccatg cagcggaacc accggcattt
     3901 tgactgcgaa ggcgatgaag aagcccagca tcaacagata ttccacacca ctggacattg
     3961 gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa
     4021 aaaccagcgc caggatggcg atcaacatca ccagaccact cgcctgggtg taaatgaaga
     4081 acttggttgc cgccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga
     4141 tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga
     4201 tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc
     4261 cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg agcagaccgg
     4321 tcagcacgac catcagcagc gacagaccgt caatggcgag atgaatagag ataccaaaac
     4381 gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca
     4441 gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat cccatggtga
     4501 tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag cacaggaagc
     4561 cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt attccttgta
     4621 aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc tcttcggact
     4681 gggggtagtg tcggatgcgc cgcgaaggca gcttatccga cctacgttct tatatgccca
     4741 ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag caccacgacc
     4801 gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct taacagcaga
     4861 cctttacctg caaagcggga aaaaaccgcc gggatgttca tcattgagtt cagcggatcg
     4921 cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc gtacagccag
     4981 tcaaagcccc aagcgttgta ccaccaggta ctcagcaggc ggcccggcgc actgttggcg
     5041 atggaggtca ccagagaacg tttacccagc cacagccagg ctgccagcag aatgccgacc
     5101 accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc
     5161 gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg
     5221 atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg agcgtgaatt
     5281 tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga ggtcataaac
     5341 gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc acccgcgagg
     5401 atctcatcct tactgaagaa gcccgcagtg accagcggca gtgccgacag tgctgcgccg
     5461 cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc catcttgaag
     5521 atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa cagcagcgct
     5581 ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg cacgccaagc
     5641 gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg tttgatgtcg
     5701 gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac aatacccacc
     5761 agatgcagaa cttccggcgt catcaggaac aggccgtggg tacgggcgat caggtagaca
     5821 cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc
     5881 gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc accgcccagc
     5941 agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt
     6001 tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa
     6061 gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt tcattgctgc tgcgccattc
     6121 ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac gccttcccag
     6181 ccgaggtaca tcagcagcag gttatcggcg agaaccagaa ctaccatgct ggcgatgaac
     6241 aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg
     6301 tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag cgacaggccg
     6361 tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt ccacagcggc
     6421 tggctgtatg cctgctcgcc gttagcgaag aaatcaacgc cgataaaggc ggttaccagc
     6481 gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga ccagcgccca
     6541 cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa ggcaagcatg
     6601 ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga cggtgaagtt
     6661 gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg gcgagaatgt
     6721 acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag gccagcgcgg
     6781 aggcattaat catgatttcc agaccaatca gcataaacag cagattgcga cggataacca
     6841 gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt tgtaagggga
     6901 tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc
     6961 acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga gcagcagcat
     7021 agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca ctgctttagc
     7081 actgattggc gtaccgtcga taccctgatc gttaacaccg aggatggcgt aaacaatcac
     7141 caccagcatg atggccgaca aaattgccgg gccaatccac acctgcggtt tcagccactg
     7201 gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga acacgaacag
     7261 caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt aagcgcctag
     7321 tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca gtgcgtgtac
     7381 cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc cacagatata
     7441 aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc gataggcttg
     7501 gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc catccggtag
     7561 aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt ctcgtaaacc
     7621 agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat cgccgtggtc
     7681 ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttaat gcggaaaaat
     7741 tccggatacc agcgaccgtc tttggtttct gctttttgca gcgagataca gccgaccggg
     7801 caggctaccg cacagaggtt acaggctacg caacgctctt cgccgtccgg gtcgcgggtc
     7861 agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg gtacattcgc
     7921 gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac ctgggtgccg
     7981 aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc gcctgccaga
     8041 gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag attttccagc
     8101 cgaaggacat tacccggtca taacgcggac gcggtaacga cgcacgaatc aaaatgaaca
     8161 tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac aacgggcctt
     8221 gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc ccgatgtact
     8281 cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa ccatccgcca
     8341 gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga
     8401 tggcaaaggt aataaaacca aagaattgcg ggatgacgtt ccacacatgc gcctggctgt
     8461 tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc agggaaagcc
     8521 cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc agcagcgagt
     8581 atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga cctgccatca
     8641 tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga ctgactggca
     8701 caattgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg gtaaagatga
     8761 cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc atgtccgcaa
     8821 ccagctggag cgaaccgccc cagccaacac ggttaggtcc ataacggttc tggaacagac
     8881 ccagcaggcg acgttcgcca aagctcatga atgccccaca ggtgacaacc accagcagga
     8941 tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat atccaactca
     9001 ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag caatgccgga
     9061 catacccatc ggcaagccca cctgccctgc cgtcagtcct tcggcgattt caaccggcag
     9121 cgtgaccgtg ttgccatcgt aactaaagga gacgcgtgta cctgcgttca cacccaactt
     9181 cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct ggaagaccgg
     9241 agcacgctgt gacaattcat cgctgccaaa caggtggtag tacggcgcga tacgccattt
     9301 cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac cgttttcgct
     9361 ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg
     9421 gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg gcacttgcga
     9481 acggtgcgcg gtcggctggt tgttaccttc catcgagaag gtgaacatgg tgtcaatatc
     9541 ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac gaccgctgta
     9601 acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc
     9661 atctttgata cccgccagtt ccgggatttt cgccacaaca gcgtcaatta catggtcgag
     9721 ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc agcgagtgca gccagcgcca
     9781 gctttccagc atgacagttt tgctgtcgta ataagccgga tcgtaaacct ggaagaaacg
     9841 ttgggcgcga ccttcattat taattaccgt accgtcgctt tcagcaaagc tggcagagga
     9901 aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga tgatcaacca ccatcaccag
     9961 cggcgcttta gccagcgcag cattcacgcg ggtagcagaa gcgtaacgat gcagatcgtt
    10021 ttccaacacc accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg cttcttcaag
    10081 cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa tcatggtgat
    10141 accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag cctgaatcac
    10201 ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac ctgccagtgc
    10261 ctgcacgatg acgtcgattt tgttttgcag ctcaggttcg ataccgtcaa ccgctggtgc
    10321 ggagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt caaccggtgc
    10381 gcggtaagtc cacgccgcga tatcatccag acgagtgtca tcaacgttgg taacaaacag
    10441 cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt cagccacttt
    10501 ctgtgctgca gccatttcgc gcgctttacc tttcaccgcc tgacgcactg ccagtgcgac
    10561 gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt aagattcgat
    10621 ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg ccagttgcag
    10681 acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca ccagttcacg
    10741 cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca ctttcttcga
    10801 ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc
    10861 atcgccacga cgctgtactg gctgacgcgg acgatccttc aggttgacgt aaccgtaacc
    10921 gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat
    10981 acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg aacattgctg
    11041 gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg agtgcgtttt
    11101 gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt cgctttccag
    11161 cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcgccataaa cgcccagatc
    11221 tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga tgcagcggtt
    11281 catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg
    11341 gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc agttaccgcc
    11401 ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga ccacactttc
    11461 acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt
    11521 catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc
    11581 acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt aaggaatatc
    11641 aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct cgtattcttt
    11701 gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcggtca gggaccgagc
    11761 gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc ccattaatca
    11821 aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga
    11881 tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt ttacccgggc
    11941 ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctggcct tcgccacgct
    12001 ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc gtacaccagc
    12061 cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc atgttgatct
    12121 catggtcaac cgccatcgcc agcgccgtac ccagacggct gcccgcttta ccgatacttt
    12181 cgaattccat cggcagatca aggtgcgctt cggtcaggaa gtcagtcccc gcgccgcctg
    12241 gctgccaggc tttaaatttc agaccatcac gcataccacc ggcgtaatct tcgaggatct
    12301 cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg atttttcacc cgaccggaga
    12361 agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac cactccacgc
    12421 cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca caggtcggtt
    12481 tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta gcacgacgcc
    12541 cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct gccccggtat
    12601 ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc aagcccgctt
    12661 cagtggcttc ggcaatggcg cggcgcagat ttactgccgc ttcgatatat tcgccacgca
    12721 ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata ccttccacca
    12781 gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc tccatttcat
    12841 cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc aggctccact
    12901 tcaggccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct tttacctgat
    12961 taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg taaccgtttt
    13021 tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc caggtcagcg
    13081 gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca gttcagggat
    13141 cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc atcatgtttg gccctttatc
    13201 acagttcccc aggcagcaag ttggcagcag cgtaaagcgg ccatcaaatg tcgtttgccc
    13261 tggtttgatg ttcagctttt tctcgagcgc cgcctgaata ccctgatacc cgttgatatg
    13321 acagaccacg ctgtcgcaat aacggatcac atggcgacca accggctggc ggaagatctg
    13381 actgtagaac gttgccacac cttcgacgtc gcttgccgga atacccagca catcggcgat
    13441 cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt tcagcgcttc
    13501 aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc
    13561 tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca taattagcgg
    13621 tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc agacaccagg
    13681 ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt gcggatacga
    13741 gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat ccccttggtc
    13801 gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga cacttgcagg
    13861 aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc
    13921 agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg
    13981 cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca gtcagaaaca
    14041 ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg acgcgcctta
    14101 cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc
    14161 gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat ggtattttgc
    14221 agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc
    14281 agacgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa ccacgccggg
    14341 tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg atcggtaaag
    14401 gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat gtacagcagg
    14461 tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac gcgatccggc
    14521 acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg
    14581 ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg gcgctcgccc
    14641 attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac aatctcttcg
    14701 ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg
    14761 ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc ttccggtttg
    14821 aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa cggcgagaat
    14881 tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt ttgcggcatc
    14941 atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca
    15001 cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt
    15061 tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat cagatggtag
    15121 aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg
    15181 ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa atcgccaact
    15241 tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt cgcctgaaca
    15301 gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc atcaagatga
    15361 tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt caccattgca
    15421 aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag gaataagcga
    15481 agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg caggttggtt
    15541 acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc gcgataaacg
    15601 ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga ttcctgcaac
    15661 agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat atacacatca
    15721 accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat accaccagag
    15781 ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc atacagacgc
    15841 tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga
    15901 cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac
    15961 gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca aattgagttt
    16021 ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt gttaacttct
    16081 tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg
    16141 ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta gcgttgacga
    16201 ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag
    16261 tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa aataaaaatt
    16321 gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca tgcgaacaga
    16381 tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa cttggcggac
    16441 aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac gtttttcgac
    16501 ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca acacaggcca
    16561 atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc agtggatgtt
    16621 gacatactca ttgcttactc atcaaaagta gcgccagatt cactgttctt cacggcagat
    16681 ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaataact gtagaaattc
    16741 ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg tcaactttaa
    16801 caaaagcttc ttcacattag tttacataat atcaacacca ttagcattta atgccctttc
    16861 accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa
    16921 gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct ccccccaaaa
    16981 gagagtattt tcttgatctg tgacacgctt ttgttattcc ataacaaaaa cgcagcaaca
    17041 aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga ttatttgtgc
    17101 attcgggacg tgaatctctg gtgattgaaa aataaacagt tttgtacgtt ctgcactatg
    17161 cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc agcggctcgt
    17221 tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat atccctttca
    17281 atcaacaatg aatcatcccc ttccggagca gacattgggc tgtattgcca cggattatgg
    17341 tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga cgggtcatag
    17401 cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact cagcacacgc
    17461 aggtccgggc tcatcatctc aaccggcctt gccgtcacgc caagaccggc tttcactgcc
    17521 gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccatggaat atctacttta
    17581 ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa tacaagaggg
    17641 atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt tggcgaggta
    17701 cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa atccacttcc
    17761 tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc cagcgctaat
    17821 ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc ggcagattca
    17881 tctgaagcac cgatagttaa cacgccctga agattactga acattaatga tgagcaggcc
    17941 tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc atgttcagtt
    18001 aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg ttccagacgc
    18061 tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc ggcagcaaaa
    18121 gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag gttaattatc
    18181 ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga gctggttaat
    18241 gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca gtttcatccg
    18301 gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact cacattgttc
    18361 cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata tctattaagt
    18421 tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt
    18481 gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag caatggtgaa
    18541 gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa aaccttttat
    18601 ttataagggt cattgcgaat tatctgatgc aaagttatgt tatgttaggc aaagtaatct
    18661 tctattatta ataagcacat caaaaccttt ttgaatatta aataataatt aattagcatc
    18721 atcctcattc attaattccg cttaacaata gtttcacacc ttacccccat tgcgacacct
    18781 cggaaatcat cacgctgtta aactctccgt aatgcggaaa tatcatcatc gcgtttcatt
    18841 agtgaattct taaatgaggc attttcacac aattatctta cagataaaaa aaccagactt
    18901 gcaattaaga attagaacaa ttcactatat aacattgcat gtaaagcata tacacctcat
    18961 tattttgtca ttattaagtt attaacagca caatcgagcc ttcccctctg gcaaaatctt
    19021 attctgcaga ccttcaaaac accgtcctgg gggagtacat tgttctaagc tgacttccac
    19081 ggcagggagt ggcgataaca gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc
    19141 agcaaattag agaatgtctg ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc
    19201 ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg gcaacccagc cccattcggt
    19261 tttgacgcgc cagatgaaat cctcgttgac gtgatacgca acctgcctac cgctcaaggg
    19321 tattgcgatt ccaaagggct ttactccgcg cgtaaagcca tcatgcagca ctaccaggct
    19381 cgtggcatgc gtgatgttac cgtggaagat atttacatcg gcaatggtgt atcggagctt
    19441 atcgttcagg caatgcaggc attgctgaac agcggggacg aaatgttggt tcctgcacca
    19501 gattacccac tctggaccgc ggcggtttcg ctttccagcg gtaaagcggt gcattatctt
    19561 tgcgatgaat cctctgactg gttcccggac ctcgatgata ttcgcgctaa aattacgcct
    19621 cgtacgcgtg ggattgttat tatcaaccca aataacccaa ccggcgcggt atattccaaa
    19681 gagcttttaa tggagattgt ggagattgca cgtcagcata atctcattat cttcgccgat
    19741 gaaatttatg acaaaattct ctacgacgat gctgagcatc actcaattgc gccgctggca
    19801 cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc
    19861 cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt
    19921 ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag
    19981 accgcgctgg gtggttatca gagcatcagt gaatttatta cccctggcgg tcgtctttat
    20041 gagcagcgta accgcgcgtg ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa
    20101 cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca aacgctttaa cattcatgac
    20161 gatcagaaaa tggtgctgga tttcctgttg caggaaaaag ttctgttggt gcaagggacg
    20221 gcattcaact ggccgtggcc ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat
    20281 atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg gttatcatca gctgtaatct
    20341 taatttcact gccggagatt gcatccggca gtgttatccc gccacaatga cctgatgatg
    20401 tcatcatacg taaggtcact atgaaacaga gccatttttt tgcccatctc tcccgcctga
    20461 aactcattaa ccgctggccg ctaatgcgca acgtgcggac ggaaaatgtg tccgaacaca
    20521 gtttgcaggt agcgatggtc gcccatgcgc tggcagctat caaaaatcgg aaatttggcg
    20581 gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta ccacgatgcc tcagaagtgc
    20641 tcaccggcga tctccctact ccggtgaaat acttcaatgc gcaaatcgct caggagtaca
    20701 aggctattga aaaaatcgct cagcagaaac tggtcgatat ggttccggaa gaactgcggg
    20761 atatctttgc gccgttaatt gacgagcatg catatagcga tgaagaaaaa tcgctggtga
    20821 aacaggcaga tgcactgtgt gcatatctga aatgtctgga agaactcgcg gccggaaata
    20881 atgaattctt gctggcaaaa acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg
    20941 agatggacta cttcatggaa gtatttgttc ccagcttcca tctttcgctc gatgagatta
    21001 gccaggattc accgctgtaa gcagccggag tctgcgtcgc atcagtcaat aagcgccgga
    21061 tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg atcatcacca cacaaaccgc
    21121 catcacgata atggtgaacg gtacccccaa cttcacaaag tcactgaagc tgtaatttcc
    21181 cggacctaaa accagggtgt taacaggtga agacaccggt gtcataaagg cggcggatgc
    21241 tgccatcgcc acgaccatcg caaatggata aggcgacact cccatcgttt tggcagcagc
    21301 cagcgcaatc ggagccatca acaccgccgt cgcggtatta gagataaaca gcccaataac
    21361 cgccgacaag acaaacaaac accccagcat catatgtggc ccataaccac cgccaatatc
    21421 catcagccct ttcaccgcca gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg
    21481 catcatccca acgatcaaaa taatgctcgg ccagtgaatg gatttatagg cgctttcagc
    21541 atctatacag cggaatttcc ccatcagcag gcaggcgatg atagcggcaa caggattagg
    21601 aatttcattt gtcagcatta acgccaccat tagcaccaga cagaaaatgg catggggtgc
    21661 ctggctgtgc gcgggcgatg cttcactcac ctcttccggt aagttcagcg ctacgaagtc
    21721 gcggccctgt ttggccagca taccgatcag tttccagtta cccacaacca ggatgatatc
    21781 gcccagcagc agaggctcat ccgccagcga accttccagc gccacgccat tgcgcttgag
    21841 ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat
    21901 cagttctgac tcaggaatta atgaaatctc tgccatgccc acatcaaggg cctggtcaga
    21961 aaaatactcg ccgcgcagta ccatcggctc cagcaattgc tcactacaaa attgccggag
    22021 atcgacatca gccgcagaca tatcaataag caaaacgtca cgcgcgcgaa attcagaaac
    22081 cccattaacg ttcacgataa cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt
    22141 agcgccataa cgctcacgta atttgagatc atccagccgt tgacctatca ttggcgatcc
    22201 ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga tattcacgga taagatcgcg
    22261 aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa
    22321 acgcatcact aacatataca aaatacccag caccagcacg accaggccaa taggtgttac
    22381 gctaaagaaa ctgaagccgt gatagccttc acgcagcaat tcactgttga ctaccaggtt
    22441 cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc ccggcaaaac ttaacggcat
    22501 catcagacgt gacggcgaag tttgcatacg catggcaacg cttaacacca cgggaataaa
    22561 gatagcgaca acgctggttg aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa
    22621 caaaaccaac attttgattt cactgttgcc cgccactttg accagccatg ttcccattac
    22681 ggtggcaaca ccggtacgga ccaaaccatc gccaataata aacaaggcgg caatcaggac
    22741 aacgttagga tcagaaaagc cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc
    22801 aaacgcgaca ataacaaaca aagcgaccgc atccatacgc actctgcccg tcgcaaacaa
    22861 gacgatggca acggccagta atgaaagaac ccaaatcaat tcaccgttca caacttatcc
    22921 ttgttaattg agggggatga cttgattctg ccataaaaaa gccccgacga gacggggcta
    22981 aatcatgatc aggtgtttca ctgaataata acatcgccat ttggctgttt ggtcacagta
    23041 atttgctcca gactgtggag gaccaaatcg acctcattca ggcgcggggt atctgccgga
    23101 gcgttaaccg caatgacatg gcaacccgcc gccaggccag aaagcacgcc agcgggagca
    23161 tcttccacca ccacacactc ctgcggcgca agccccagca gctgcgcacc taacagatac
    23221 gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg ttacaaacac ctctggtgcg
    23281 ggaagccccg ctattttatg gcgcgctcgc gctaccggca tggagccaga agtcacaatg
    23341 gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc aggaagcgcg
    23401 gtaatacctt cggtttccgt ggcctcgatt tgctccagac gcgtaaactc ggcggcaata
    23461 tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg tgatcgcctg tttaccgtga
    23521 atgaaagcca gcacctcttc cggcgctaac ccatgacgtc tggcccagtt gctccacgcc
    23581 cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag aaaacctttg
    23641 caccgcacgc gggcctcctc aggcgttgat aatttgatta atttcgttgg cgctcaaatg
    23701 gtactgacgc gggcaggcat gccacacatt aagcatgcgc tgatattttt cccacattgg
    23761 cgtctgggcg ttaaaaccat gagtcccagc gtcaaaatgg gtatagcgcc cttccacatt
    23821 aaccataaag cggacataac cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa
    23881 ggtgacacga cgttcatcga tggattgctg gtcctgcaaa ttagaccagg aaacatacaa
    23941 cgcatgatac atctccataa tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc
    24001 aaactcgcga tccaattcac gcatctgtaa tccgtaacca cgctcaataa ttgtttgcag
    24061 gcgacggtaa cgttcagcat ttgccggatc gagcatagtc atcatcttgt actgattaga
    24121 caaaataaga cgttgcgcgt tggtcatttc cattgttgac tcctgtatca ctctactacg
    24181 gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg gtcaattaca
    24241 aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg
    24301 cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc tgcaatttac
    24361 cgcgaacttc ataaaaccag ttaaggattg cagggggtaa tggcgttaca gaacgcttgc
    24421 ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagtgcagtg gcaactgccg
    24481 gcccaagcag accgcccagg gcaatctgcc agcagagagt aaatacggcg atcggcggca
    24541 taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag accggggcaa
    24601 ggcgtttttc cagtggccac gtctttgagt aatgctgtcc gcggcgaaac aagctaaaaa
    24661 aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca tataaagatt
    24721 caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt tgtcttccat
    24781 acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat tcattgagtc
    24841 gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca tagactcaag
    24901 atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt
    24961 gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta tcaattatag
    25021 gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct tcactgaaat
    25081 ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc gaatgtttcc
    25141 acctgcccga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa gcggctttag
    25201 gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt ctggcacaaa
    25261 aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa
    25321 agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa gatgcagctt
    25381 cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct ctgaaatctt
    25441 tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac cagactatgc
    25501 cggaagagtc ttacctctac gccctgccgt acaacctgta caaagagcac ggcatccgtc
    25561 gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca aaaatgctga
    25621 acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt ggttccgttt
    25681 ctgctatccg caacggtaaa tgcgttgaca cctctatggg tctgaccccg ctggaaggtc
    25741 tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac ctgcacgaca
    25801 ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct ggcctgctgg
    25861 gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg acgaaagaag
    25921 acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc ggtgcctaca
    25981 ctgcgctgat ggatggtcgt ctggacgctg ttgcattcac tggtggtatc ggtgaaaatg
    26041 ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt gaagttgatc
    26101 atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa gaaggtaccc
    26161 gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac gcgagccgcc
    26221 tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa cccgccaaat
    26281 cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac
    26341 cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt
    26401 tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca
    26461 gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac cgctgaaaat
    26521 gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg aagagatcgt
    26581 cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc
    26641 gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa cgctgaatgc
    26701 ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg gaacagctga aagagcgtat
    26761 cgaactgacc cgcaacagct tcggtggtgc caaaaacacc aacatcaccg gcgttatcgt
    26821 taacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccggatc tgtccgagat
    26881 tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc
    26941 cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc
    27001 gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca tcaatactcg
    27061 ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg agcacttccg
    27121 tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggtgg ccgcttgcct
    27181 ggcagccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct acgaaatgga
    27241 cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt
    27301 gaacaccaac acctggcaga cctctctgag cctgcagagc ttcaacctgg aagttccggt
    27361 tgacgatcac gaacgtatcg agaaagttca ggaatacgtt gctaactaca tcaacgctga
    27421 ctggatcgaa tctctgactg ccacttctga gcgcagccgt cgtctgtctc cgccagcgtt
    27481 ccgttatcag ctgactgaac tggcgcgcaa agcgggcaaa cgtatcgttc tgccggaagg
    27541 tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta tcgcaacttg
    27601 cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact
    27661 aggtgcaggc attgaaatcg ttgatccaga agtggttcgc gaaagctatg ttggtcgtct
    27721 ggtcgagctg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac agctggaaga
    27781 caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc tggtttccgg
    27841 tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca aaactgcacc
    27901 gggcagctcc ctggtatctt ccgtgttctt catgctgctg ccggaacagg tttacgttta
    27961 cggtgactgt gcgatcaacc cggatccgac cgctgaacag ctggcagaaa tcgcgattca
    28021 gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc gttgctatgc tctcctactc
    28081 caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc
    28141 gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg ctgcggtaat
    28201 ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt gcaggtcgcg ctaccgtgtt
    28261 catcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc gttctgccga
    28321 cctgatctcc atcgggccga tgctgcaggg tatgcgcaaa ccggttaacg acctgtcccg
    28381 tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc agtctgcaca
    28441 gcagcagtaa tctcgtcatc atccacagct ttgcgctgcg gatatctgaa ccggaaataa
    28501 tcactatttc cggtttttta ttctcttaat ttgcattaat cctttctgat tatcttgctt
    28561 aactgcgctg catcaatgaa ttgcgccatc tcactttgca tacttaccac tttgttttgt
    28621 gcaagggaat atttgcgcta tgtccgcaat cactgaatcc aaaccaacaa gaagatgggc
    28681 aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagtc ttgccacctg
    28741 ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg atggtcaaac
    28801 aaaaacacgc aaagtcgtag atccacactc atttcgcatt ctgactaacg aagcaggcga
    28861 acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg gcctgatgaa
    28921 cttcccgttt gaagggttaa cctcaggatc gaaatacggg acagccgttg gcatcatcat
    28981 gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa
    29041 cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta ttcctgcgct
    29101 gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg ccgtcgcctt
    29161 tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta ccaccgtcct
    29221 ggtgacttat attgccacgc aaatcggttt tgccagttcg tggatgaacc cgttttgtgt
    29281 ggttgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt
    29341 ggtgtgggtt atcgccactc tgattggcct gatctttacc atggtgtacg cctcacgagt
    29401 gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca
    29461 ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga ttgtcctgac
    29521 agccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta ttccagaaat
    29581 tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg ttttccgcct
    29641 taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcgc gaatgatgat
    29701 cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggttg gtaatggtga
    29761 agcaggtaat gccagcgtgt taaatactat cctcaacagc attgccaatg ccattagcgg
    29821 tctggacaac gcggtcgcgg cctggtttat gttgctcttc caggctgtat ttaatttctt
    29881 cgtgacgtcc ggttctggtc aggcggcatt aactatgccg ttactggcac cgcttggcga
    29941 tctggtcggt gttaaccgtc aggttaccgt gctggcgttc cagtttggtg atggcttcag
    30001 ccacatcatt tacccaacct cagcttcgtt aatggcaacg ctcggtgtgt gccgggtgga
    30061 cttccgtaac tggctgaagg tgggcgcgac cctgcttgga ctgctgttta ttatgtccag
    30121 cgtcgtagtg atcggcgctc agttgatggg ctaccactaa aaatgttaag agccgcaatt
    30181 gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg cattgcgctt
    30241 catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat
    30301 ttcttccggc gtcagccagc aaactccact gacttcatct tcctgtaggg cgaagggacc
    30361 gtgagagacg caactgaaca acgcgcccca gacacggcaa tttttatctt cgaaatagaa
    30421 ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac tcttcttccg cttcgcgacg
    30481 cgcggattcc agcagttgct catcggcctg aactacaccg cctgcggtcg catctaacat
    30541 gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc ccatgccatc
    30601 atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt gttcccggct
    30661 ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag tacttgccaa
    30721 acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg cgcattttga
    30781 gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat cattgagtgc
    30841 gattacacta agtacgtcat tatccagcat gccataaccc gccggattac cgcctttcgg
    30901 aatactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg ccactggcag
    30961 atgggtatga ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc
    31021 aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct gttgccacgg
    31081 cgcggttatc gggaaatgca gcatcatttg atccacttcg ctgtcgcagt tgccacgcac
    31141 agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg gcgcgtaacc
    31201 ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca ccagccactg
    31261 ggcaccgctt tggacaaaca actccacaac acgttccgtc gccggtaacg acccatgaat
    31321 gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta agctatgata
    31381 ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata aaacgctgga
    31441 cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcgttctggc acctcatcgc
    31501 catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg caccattcac
    31561 caaataaatt cggctgaccc agtgctaaca aatgttctgc catcgcgaac agtttctctg
    31621 cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg
    31681 tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg
    31741 cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc
    31801 gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta
    31861 ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc
    31921 tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccagg
    31981 cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac
    32041 tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct ttttgtcact
    32101 gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga
    32161 ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa
    32221 aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacctggg
    32281 gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc
    32341 aattgttgat cattctcctg ccgatggcgg cgaaccgtta agcctctttg agtctggtgc
    32401 cattttgttg tatctggctg agaaaacagg actctttttg agtcatgaaa cgcgtgagcg
    32461 cgccgccaca ttacagtggt tattctggca ggtaggcgga ctggggccga tgcttgggca
    32521 aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca
    32581 ggttgaaact cagcgtcttt accatgtact aaacaagcgg ctggaaaact cgccctggct
    32641 gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac
    32701 tcgcctgcga attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg
    32761 ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg agcgttcgga
    32821 tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct
    32881 gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttgcg tacgtttatc
    32941 ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac
    33001 taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc
    33061 gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgct ggaaaaatta
    33121 actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag
    33181 atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag
    33241 cgttaatcgc catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa
    33301 ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaattacg gtagtgacgc
    33361 gtaacccgca gaaagccagt tccgttctcg gccctcgggt gacactatgg caagggcttg
    33421 ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg
    33481 ctgataaacg ctggactcac aagcaaaaag agcgtctctg ccaaagccgc tggaatatca
    33541 cgcaaaaact ggtcgatttg attaatgcta gcgacacgcc gccgtcggta ctcatttccg
    33601 gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc
    33661 cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc
    33721 aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg
    33781 gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attggttccg
    33841 gtcggcagta tctggcctgg attcatattg atgatatggt caacggcatt ctctggctgc
    33901 tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccggta cgcaatgaac
    33961 aatttgccca tgcgctcggt catgcgctgc atcgcccggc tattttgcgc gtccccacaa
    34021 ccgccattcg gctgttaatg ggcgaatctt cagtactggt attaggtgga caacgcgcgc
    34081 tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc
    34141 tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa ctccctgtgt
    34201 tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc accatcataa
    34261 ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgt
    34321 gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct
    34381 tttcttgtgg tgcgcgttct gttatctcac caataaatgc acggaaacgc tgggcatccc
    34441 ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa
    34501 ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct
    34561 gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc ggcaccacgg
    34621 taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca ggctcggcaa
    34681 attcatcaag ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca
    34741 tcgggatcac caatggaagc tctttatagc ccgcatcaag atggttttgc attgccgcaa
    34801 tggaataacg catcatgcga aaagccatta attcttccgg cttactttgg tgctctatca
    34861 ctacataaat ataacccact ccctcctgcg ttttcacaga ccacaagagg tcggaataat
    34921 attgccgcag gtcttcatca ataaaactgt ttggttctag tttaagcgtc gttaaatcac
    34981 acagtttgcg cagcggcgcg ggaagatgaa tattaataaa attccgcgcg gtgtctggat
    35041 ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga agttgtcgat attgtcatgg
    35101 cgttccgtca cccctcaaat ttaagatgac gcgacaataa ccacgattaa cacccaatgc
    35161 agcaggcagt ttttatcttt ggacagcgaa ttcagaagta tttttagcgg actgcaactt
    35221 ttacatttca gcggaagctg actcgcaaat tccccttgaa aatactccgg cagcgttagc
    35281 caccggagag gaaattattt cagcgatccc ttaaggaact gttgcagacg agggctttgc
    35341 gggttgccaa ataactgctc cggcgcgccc tcttcttcta ttttcccctg atggaggaaa
    35401 atgacgtgag tagaaacatg gcgagcaaag cccatttcgt gagtcactac caccatggtt
    35461 ttcccctctt ctgccagttg ctgcataata cgtaacactt cgcctaccag ttcaggatcg
    35521 agcgccgagg taggttcatc aaacagtaaa acttctggtt ccatcgccag cgcccgcgcg
    35581 atagaaacac gctgttgctg accgccggaa agatgcaccg gatatttccc ctgcgcacgt
    35641 tcgtctatcc cgactttcgc cagatacttc accgcccgct cgcgcgcttc ctgcttgctc
    35701 aggcccaaca cctgaatcgg cgcttccatg acgttttcca acaccgtcat atggctccag
    35761 agattgaagt gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta
    35821 tcggcgactt tgagttgacc gtctttgtcg cgcaccagat tgatcgtctg gccattgacc
    35881 acgatcgatc cttcactcgg tttttcgagg aagttaatgc agcgcagaaa ggtacttttc
    35941 cccgatcccg acgatccgat gatgcttatt acatctccgg cattcgcttg cagtgagacc
    36001 cctttcagca cttcgtgttc gccatagcgt ttgtgcaaat cgataacgtt taatttattc
    36061 tcggacatcg tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc
    36121 cgctctgcga aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat
    36181 gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta ttttcagcag
    36241 atccggcaca gtggccgtaa acgccagcgc agtagagtgc agcatcagga tcacttcgtt
    36301 gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcagc gatacatttt
    36361 aaaggtcgag aagccatagg ctctggcggc ttcaatttcc ccatgcggaa ccgaacggat
    36421 tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt
    36481 acagttcagg ccactgcgga agaaagcgtt aaggaattcg gttcccttaa caatctcaag
    36541 cgtgtacatg ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat
    36601 ataggtaaat aaccagattg gaaactggat gtatttatta ctggagacac gaccaatcgc
    36661 cagaaacagc gccaggactc cgcctatcac taccgacaaa ataagcagcc acagagtgat
    36721 cgccacacca gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt attcatgtaa
    36781 gatttcgatc acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc
    36841 agcacaccat tggaaacggt ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa
    36901 tagaacggtt cccaggtact tttgcctgcc agctgcgtgg ctttgaccac atcttccagg
    36961 ccgagtaacg aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct
    37021 ggcagggcgt aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccgcgagta
    37081 aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca ctgccataaa cgcgccacgg
    37141 aacgtttcgg taaaataagc accgtagata aaaccgagag tgataatacc agcgaccatc
    37201 ggatcgatat caatctgccc gacgcccatc gcctccgtca ccatgtttag cgcaatctgc
    37261 aaaccataga aaatcagcag catcaacacc aagtccggca cgcctcgaat cagcgtggtg
    37321 tacccttcga aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt
    37381 aaaccgatga ttacagcgag cactacagag ctgatagcca gctccagcgt gacgagcgca
    37441 ccctgtaaaa taacacctga aaacccatac aacatgctgc ctgtcctgtc gtgtgtggtg
    37501 aattaccgcc tcttgtctcc ctccggttac ccgaagggag cgggctttct ttcaccgatg
    37561 gcgattaacc accataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac
    37621 cgtcagcgcg catttcggca aaggctttgt tcagtgcctc gcgcagttcg ttatcttctt
    37681 tacgcaggcc catgccggtc cctacgccaa acagtttttc atctttaaca gacggaccac
    37741 cgaatttgta atctttaccg acgggttgtt tgaggaaacc ttcgctggca gcgacctcat
    37801 cctggaacgc ggcatcaata cgtccggcag tcaggtcaga ataaatgttg tcctgcccct
    37861 gatacgagac gatttcaatg ccttttggtg cccagtgttc attaccgaac gtctcctggg
    37921 tggtgccctg caatacgcct acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt
    37981 cagaattttt cgccaccacc agacgagaat cggcagcgta cagtttgtcg gtgaaggcaa
    38041 tttcttgctg acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg
    38101 cttttaagga cgggattaac gcatccagcg ggttttcgac aaacgtacat tgcgtattga
    38161 tgcgcttgca caactccttc gccagctcga tatcgaaacc aaccagttcg ccttgtgaat
    38221 tttttgattc aaatggcgca taggtcgggt cggtaccgat gcggatgttt tgcggaattg
    38281 cagcaagtgc cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt
    38341 ttttcataat gtcctcaact gacagtcttt ttatagggaa tatttacagg tttgttacac
    38401 ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc gccccgttag acgtattatt
    38461 tttcattaaa aatgcttaag tatgtatttc aatgcaccac gatagtgcaa caaacctggc
    38521 gtgcaccatg atgatcattc catcaggtac agcttcccag cgacgtatca gtcaccgtag
    38581 acattaaagt cgaaatactt tttcgccatc ttgtcgtagg tgccgtcctg acgcagctcg
    38641 ccaagcgcct tattgaaggc agccgtcagt tcagcatcat ctttacgtag ccctacaccg
    38701 gtgccatcac cgaagtattt tttgtctttt actgatgagc cagcaaaagc gaaatcttta
    38761 ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt catcttgtaa cgcagcatcc
    38821 agacgtcctg cagccagatc ggaatagacc aaatcctggt tggcataggc caccacatcc
    38881 acgcctttac tacgccaggt ctcgttagcg taagcttcct gggttgatcc ctgcagcaca
    38941 ccaacatgtt tacctttcag tgaatccagc gttggctgaa tcggtgaacc tttggcagca
    39001 atcaaacgag aatctgcggc gtacagcttg tcggagaagg caatctcctg ctgacgttta
    39061 tcggtaatgg aaagcgacga aataatagcg tcgatttttt tcgctttcag tgaggggatc
    39121 agcgcgtcaa agtcactggc aacccaggta catttcacct gcatccgttt gcacatctcg
    39181 ttaccgagat cgatatcaaa gccaacaaaa tcacctttag catctttcga cgagaacagc
    39241 gcataggtgg tatcggttcc gatacgtacc gtctccggta gcgccgcata gctggaagcc
    39301 gctgcggaga gaccgactaa taaagacaga gcgagaatcg acttcttcat acataaccct
    39361 caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg
    39421 caggtcttat gccatcttga cgacaacagt aacattcaac gttaaatatg ttaataagac
    39481 gttgcattat tgtcctgaag ttgaagatag caggtatagc ggttgaatcg cagcgtttcg
    39541 attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac
    39601 agggcaacag cggagttatg cgccctgcca gcgggcaaag agatcttcag gaagggttat
    39661 tgcaaactgg tcaagaacac gattaaccgt ctgatttatc acatcatcaa gggattgcgg
    39721 gcgatgataa aacgccggaa cgggaggcat aatcaccgca ccgatttctg ccgcctgagt
    39781 cattaaacgc agatggccta agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg
    39841 acgctctttc agcaccacat ctgccgcacg ggtcagtaag ccatcagtat agctatggac
    39901 aatgccggac agggttttga ttgaacaggg taaaatcacc atccccagcg tctggaaaga
    39961 accggaagag atgctggcgg aaatatcgcg cgcatcgagc gtgacatcag ctaatgcctg
    40021 cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc tggcgcgccg cctggctcat
    40081 caccagatgc gtttcgatat ctgtgacatc gcgcagaacc cgtaataagc gcacgccata
    40141 aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt ttcataaata gttgccctgt
    40201 cagacttgca ggcagacttt gcagtatttc ggcgggagtt gcaagtcagg gtgccagacc
    40261 ggcaccctca gcgaaggcat catccttcgt tatgcatttc gagattttcc acttcgttct
    40321 gacgttgcac tgctttggcg tcatcattac gtaacgtatc gaggaaatcg aggtagccct
    40381 gatcaacatc tttggtgacg tagacgccgt tgaacaccga gcattcaaac tgctggatat
    40441 ccggattttc agcgcgaacg gcgtcgatca gatcgttcag atcctggaaa atcaacccgt
    40501 cagcaccgat gatctggcga atttcatcaa cttcgcgacc gtgagcgatc agttccgtgg
    40561 cgctcggcat atcaatacca taaacgttcg ggaagcgaat ttccggtgcc gcagaagcga
    40621 ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc
    40681 cacggacgat ggagtcgtcg accagcagga cgtttttatc gcggaactcg gcgcggttgg
    40741 cattcagttt acggcgcacg gacttacgac gcagctgctg gcccggcatg ataaaggtgc
    40801 ggccaacata gcggttttta acgaagccct ggcggtacgg tttgcccaga atacgagcaa
    40861 tttccagcgc gatatcacac gaggtttctg ggatcgggat caccacgtcg atatccagat
    40921 cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc catattcaca cgcgcgctgt
    40981 aaacggaaat tttgtcgata aacgagtccg ggcgggcaaa gtatacatac tcaaacaggc
    41041 acggattgct gaccggattg tcagcacatt gacgggtaaa caactgccct tcttcagtga
    41101 tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg
    41161 ctacgctttc ggaagcgacc atatattctg tacggttctc gtcaatatca cgttttccca
    41221 gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac cataccgtgg ccgataatca
    41281 tcgccacaca ggcatacgcg ccgcggatta agcggtttgt ggcagcaatg gcagcgaaaa
    41341 tattgtcggc ttccagcggg tagtggcgga agttgtccag ctcgctggcg aagatattaa
    41401 gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg tttttcttca aacagttttt
    41461 tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc aagcgtaatg ccatacgggg
    41521 agttaacgta aaacggctgc gcttcagagg cgctggagct gccagccgta gggtaacgca
    41581 catgaccaat gcccatattg ccctgcaaac gctgcatatg gcgagcttca aatacatcgc
    41641 tcaccagccc gttcgcttta cgcaaacgga agcagttatt ggcatctatg gtgatgatgc
    41701 cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc atcataaatc gactggttaa
    41761 ccggcataac accggcgata ccgacaatac cgcacatacg tctttttcct cgttaagcca
    41821 catctcagag cacttacgct ctgggcaaga aacttgacga gctttgcaga taatcaaaaa
    41881 accatctgat gataaaactg aattgcggga tcagctgtga tttgctccag tcttcacttt
    41941 tcgacacccc ggtaaaggag tcgagaaaga agagaatggc ggcaacaatc aacacaccgc
    42001 gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc tgacaacccc gttttctcca
    42061 ccaactggcc tatcacgaag ttcacgatag caccaacgat cagggtagcg ataaacagta
    42121 ccgcgatggc aatcccattt cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca
    42181 ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc ccatgtcacc agcgataacg
    42241 cttcacgaac aaagccgtgg atcaggctaa ccagagagga aaaagcaatc accgcgatta
    42301 tggcgtaatc aatccagacc atatgtgtcc cacgatttta cgccctgtca tcctgttcgg
    42361 ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat ttccttcccg cgcatcaata
    42421 aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt atgcctttgc ggcatcgtgc
    42481 aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta cggttctaac actgcccaag
    42541 cctgataaga cgcgccagcg tcgcatcagg caagactgta ttaattcggc gtatagccca
    42601 ttaccacgcc acttaagcca gaaagttgct tcaactcacc cagcgaacct ttcagcttat
    42661 ctttcgaggc atccggccca accagaatac gggtaatttt accctgcact ggcgtggatg
    42721 gcgacgtata aacccgataa ccggcaccgc gcagcttacc gacaatctca ttcactttat
    42781 cggcattttt cagcgcaccc agttgcacaa cataggcttt acccgttggc gctgcttttt
    42841 cttccacgac cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc tttggtttag
    42901 gcggctccac cggtttcggc ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg
    42961 tgttattggc tgcaatagtg gccggatcga gcgacggtgc tgcggcatca cctgcccgca
    43021 cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg gctggcatca
    43081 tatcaggctc atcacgatcg cccgctttcg gcaccagcgg gatagccgcg aactcatcct
    43141 gataatgttt tttctgcccg tccagcagcc ctggaagtac aatcaccccc agcgccacca
    43201 gcacgatcgt gcccactaac cgattctgaa acttacttgc caccgcttct cctcgcgtca
    43261 atcacttcca tgacatgtgc gaccgtgtgg aaagaaccac acaccagcac ggtgtcttcc
    43321 gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg
    43381 ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca
    43441 caataccagt catcaaccac gcttttcaac caggccagag ttccggcaat atctttatca
    43501 tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg
    43561 gtgagatatt ccgccgcatg tggattatgc gcgacatcaa aaataacgcg tggcgactcg
    43621 ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt
    43681 tcactgactt ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc attcggttgc
    43741 gggacaagcg gcaacggcag attttccagc gtgccgtgag catcgctaaa cgcccagtca
    43801 tgatcggtga cggaatagtt ccactcaacg ccccgacgtt gtaacagtgc acctttttcc
    43861 tgcgccacat cagcaatggt agaaggcatt tccggctcac cgacaattgc cggtttttcg
    43921 ctgcggaaga tgcctgcttt ctcgcgacca atactttcgc gatctggacc cagccagtcg
    43981 gtatgatcca gcgcaatact ggttactacc gcgacatcgg catcgacaat attggttgcg
    44041 tccagacgac cgcccagccc tacttccaga atcaccacgt caagttgtgc ctgcttgaac
    44101 agccacaacg ccgacagcgt accgtactcg aaataggtca gggaaatatc accgcgtgcc
    44161 gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg gcaattcctg gccctgcaca
    44221 cgtacgcgct cggtataacg caccagatga ggcgaactgt agacgcccac tttgtaccct
    44281 gccgccatca gaatcgactc cagcgtacgg caggtggtgc ctttgccatt cgtacccgca
    44341 acggtaaaca caaatggcgc tggtttcagg acgccaagac gcgccgcgac ctggctcacg
    44401 cgctcaaggc cgagatcgat agttttactg tgcaggtttt ccagataaga aagccacgaa
    44461 gccagaggcg acgcggcttg aggagtgcgt ttgataatca tggtatccgc tgattcgtta
    44521 cggtgagaat agcaaaaggg cagagccagt ggccctgccc ttatcagtta tcaggcctca
    44581 ggttcctgat ccggtaccgg gggtaccact acgccttcac gcggcgcttc aggattcggc
    44641 gctggcagat tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat ttccggacga
    44701 cggacgatca tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg gaatctaggc
    44761 ggcagttttt cgcgaacggt ctgttcgata acacgcggac cggcaaagcc gattaacgct
    44821 ttaggttcag cgatgttgag atcgcccagc atggcgaagc ttgcagaaac accgcccatc
    44881 gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct cctgcatttt tgccagtgcc
    44941 gcagaggttt tcgccatctg catcagcgac atcagtgctt cctgcatacg tgcgccacca
    45001 gaggcggaga agcagatcag cgggcagtta tcttcctgcg cctgctcaac ggcacgcacg
    45061 aaacgtgcac ccacaacaga ccccattgaa ccgcccataa aggcgaactc gaatgccgca
    45121 gcgacaaccg gcattccata cagagtgcct ttcatcacca ccagcgcatc tttttcgccg
    45181 gtttctttct gcgcagatgc cagacggtct ttatacttct tggagtcacg aaacttcagc
    45241 tcatctttcg gctcaagctc gctacccagc tccacaaggc ttccttcatc taacaggcta
    45301 tgcaggcgat tacgcgctgt catacgcatg tgatggtcac acttcggaca gacctcaaga
    45361 ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc tatcacactt agtccacacc
    45421 ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg ttcaatccag
    45481 ctcattaggg acctttctgt ctgaacctgg ttcgatgcca gttttatctt tggggacgca
    45541 taatgccatt tttgccccca acagaccatg aatgttgcac attaaaacat aacagcccga
    45601 aactttggat aaaaaagtgg tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg
    45661 cagcgcgttt gtgacggatt atttcgatga cgcccggcaa aatagaaacc acaataatcc
    45721 cgacgatcag cagcttaagg ttatcctgaa ccatcgggat tgtaccgaag aaatagcctg
    45781 cgtaggtaaa aagcagtacc cacaacagtg cgccgatcac gttataagca gcgaaatgac
    45841 ggtacgacat gtggcccatt cccgcaacaa acggggcgaa cgttctgacg atcggcacaa
    45901 aacgggcgag aataatcgtt ttgccgccat gtttctcata aaactgatgg gttttgtcga
    45961 gataactgcg acggaaaatt ttcgaattgg gattactgaa taacttttca ccgaacaacc
    46021 gcccgatagt gtaattgacc gcgtcaccca caatcgcggc gatcagcatc agcactacca
    46081 tcatatggac gttgagatcg ttggtttcca gtgatgccaa cgctccggca acaaacagca
    46141 atgaatcacc cggtaaaaac ggcattacca ccagaccggt ttcacagaac aaaattaaga
    46201 acaaaatggc ataaacccag acgccgtact ccgcgactag ctctgccaga tgcacgtcaa
    46261 tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat atttgcctat gccttgtact
    46321 cgtcatcttt caggctgtaa ctacgttggc tacagcctga aatgttccga gcattattct
    46381 cgttagtccg ccagaaatag cgggcccatt ggcggttttg gaagatcata ccggtcaggg
    46441 taatccaccg cgaccagata cagcccttcc gcttttgccg ttgctgccgc cagcgttctg
    46501 tcctttgccg ccagcaactc tgctatccag ctctccggct ggttgtgggc accgacttcc
    46561 atcaggctgc cgacaatatt cctgaccata tgatgtacaa aggcattcgc tttgatatca
    46621 accaccacat aaggaccgtg acgcgtgaca ttaatatgca taacgttgcg ccacggggtt
    46681 cgggactggc actgcaccgc acggaacgag gtgaaatcat tctcgcccag caagcattgc
    46741 gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa aatgggttac ccctttactc
    46801 agtaccgccg ggcgcagccg atgattgtag atgatgtagc gataacggcg agccgtggcg
    46861 ctaaatcgtg catgaaaatc atcaggtaca gctttaaccc aacgcacggc gatgtcacca
    46921 ggtaaattcg catttacgcc caaggtccac gccgcgtctt tgcgctgcgc ggttgtttcg
    46981 aaatgcacca cttgcccggt accgtgtacc cctgcgtcag tacgcccggc gcagaagacg
    47041 gtgatgggtt cgttcgccac ctgggagagc gccttttcca gcttctcctg cacactgcgg
    47101 acttcattct gccgttgcca gccgtaatac ttactgccgt cgtactcaat gcccagcgca
    47161 attttataaa ctggcagttg ttgctggtcg gacattagta cagatactcc tgcaccagtt
    47221 tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc
    47281 agaactggac ttgctccggc ataccgtagt cattacgcac gcagccaaca gaaagatgcg
    47341 gcgtacccga agcatcccct acctgagtcg ggaattcgtt ctcttcagag agcacaatat
    47401 cttcgccctg agcaaacgca tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa
    47461 agttgaccat ctgggcatga ccgtagaata ccggtgcctg gacgacgctt gccgaaatca
    47521 tcagcccttc gtcctgcagg attttgcgta cttcgtcaac gatacgacgt tcttcacgca
    47581 cgctaccttc gctatccggc aacaacggca gcatattgaa cgccagttga cgaccgaaga
    47641 aatcttcttc gtcaatcgga atgccgttga gcaatttcgc actctgcccc gctaacgcat
    47701 cgaccgcttt tttgccctgg gcggaggctg aaatcaggct ggtaacactg atacgcgaca
    47761 aaccgccctg atcgattaac ggtttcagtg ccgccagcag ctggctggtc agactgtctg
    47821 gtacggcgat gacattccgg ttgcggtaat cggtcagtac aaacgggttt acttccggca
    47881 ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct gctgtcgatc accaggcaac
    47941 ctgagttggt cgcttcttca acccaggcag cggtagcttc tttgcctgcg acaaaaaatg
    48001 ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg cacggtgatt gttttaccac
    48061 caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag tgcataaatt tccccaaccg
    48121 ggaactgacg ttcagccagc gtttcaagca gggcttcgcc catagcgcca gttgcgccca
    48181 ggacggcaat gttccagcct tcagacatgg tggtttactc cagaaatagc aaagctccct
    48241 gccaatgtta cagcagagag catgaagaag agattaacgt gccggatgat gaacggcgtt
    48301 aaaacccagt ttacacagca atgatgccgc actggcgtca tcacaaatta catacagaga
    48361 tgaccattca cggcgctcaa gatagttttt gcgcagttta tcgaactcac ccggtatccc
    48421 ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct
    48481 tttcagcgtc ggttgatcga gcggaccatg cagcgtaatg cggccaaact caggcgcggg
    48541 cagcaacgta tccagcgcaa cgtgttgttc atgcccaata aacttgctat aggcttcaaa
    48601 cacttgcgtc gtgccacgcg ctttaccctc cagggtatag cctgcgatat gcggcgtgcc
    48661 gatatccact tttgtcagca attccacatt aagttccggc tcgccttccc agacatccag
    48721 caccacgctt aacttctggc cttcattcag gcaggtcagc agcgcggtat tatcgacgac
    48781 tgcgccacgg caggcgttaa tcagaatcgc tccgggcttc aggctacgga tcagtttttc
    48841 atccgccaga tgtagcgttt tgtacggacc atctttaaag agtggcgtat ggaaagtcag
    48901 aatatccgcg cgctggacta actcatccag cgagcggaaa tcaccctcat ccccacggtc
    48961 ggcgcgaggc ggatcgcaaa gtaaggtttt aatccctaac gcttccagtc gcgcctgcaa
    49021 tcgacgtcca acgttaccaa cgcccacgat ccccaccgta cggtcgtgca gtgaaaatcc
    49081 atcgcgttcg gcaagcatca gcagggagga gaaaacatat tccaccaccg caatcgcatt
    49141 acagccaggt gcagcggaaa aaccaattcc cgcctgcttc aaccatgctt catcgacatg
    49201 gtcggtcccc gcagtggcag ttccaacaaa tttaatgggt tttcctgcca gcaaagattc
    49261 attcactttc gtgaccgaac gcaccatcag cgcatccgcg tctgccagtt gagcgacggg
    49321 gattggacgc ccgggaaccg cgatcacctc acccaaacgg ctaaataagt cgcgggcata
    49381 aggcatattt tcatcaacaa ggattttcac gtttgtgtta cctgtatgag acgagagtta
    49441 accggacaag tatgccataa tctcgcggcc aggcatactt gcgaagattt caggtataag
    49501 gatacgtaat gatacaacct atttccggcc ctcctcctgg gcagccacca ggtcagggag
    49561 ataacctgcc gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt acttcgctgg
    49621 aaagcttaat gacgaaagtg acctcactga cgcaacaaca aagagcagaa ctgtgggcgg
    49681 gtatcaggca cgatattggt ctgtcgggag attcaccgct gctttcgcgt cacttccctg
    49741 ccgctgagca taatctggcg caacgtctgc tggccgcgca aaaaagccat tctgcccgcc
    49801 agcttttagc gcaattaggg gagtatttac gtctggggaa tcatcgtcag gcggtcacgg
    49861 attatatccg tcataacttt ggtcagacgc cgctgaatca gctctcaccg gagcaattaa
    49921 aaaccattct caccctgttg caggaaggga agatggttat tccgcaacca cagcagcgcg
    49981 aggcgaccga ccgtccttta ttaccggcgg agcacaatgc gctaaaacag ctggtgacca
    50041 aacttgcggc ggcaacgggg gaacccagca aacagatctg gcaatcgatg ctggaacttt
    50101 ccggggtgaa agatggcgag ttaattccag cgaaactgtt taaccatctg gtgacctggc
    50161 tacaggcgcg tcagacgcta agccagcaaa atacgccgac gctggaatca ctacagatgg
    50221 cgctaaaaca acctttagat gccagtgaac tggcggcgtt atcggcatat atccagcaaa
    50281 aatatggcct ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat attcttaatc
    50341 agctttatca acggcgggtt aaagggattg atccgcgtga tatgcaaccg ctgcttaatc
    50401 cttttccacc gatgatggac acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac
    50461 tgttagtcgc gattatcctg atgctggtct ggctggttcg ttaaccccga cgaaatgaca
    50521 gtatcgtgac aataataccc agcaccgcag agatcgcccc ggcaagaaat accgaagagt
    50581 aaccaaacgt ggtcgccagc attcccgcca gcggcccgga gacgccgagg gcgatatcct
    50641 gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca
    50701 cctccacgcc cagcgcagga aagataagcg aacatccggc tccggttaac gccgcgcccg
    50761 ctaatgcgac ccatgcacct ggggcttgcc agagcagcaa caagcccacc gtttctacaa
    50821 gcagagagac aatcgccact ttcacgccgc caaaacggtc cggcatccag ccaaacatga
    50881 cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc
    50941 ctttgctggc aaagtagagc gaaacgaaag tcccgataac cgcaaaacca acgccttgta
    51001 gtgccagccc taaccctggt ttccagataa gcccgacaac gctccacagc gatggacgtt
    51061 ctcccgccag ggccggtact ttgcgcactg tgccgttaca ggcccacgcc agtaagggta
    51121 ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa
    51181 gcggcgcacc aacagcgagg gcaccgtaaa tcgccattcc gttccatgac atcactttgc
    51241 cagagtgttt tggccctacg atgcctaacc cccaggtcag agcgcctgtc agtaactggc
    51301 tttcaccaaa cccaagaatc aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg
    51361 agacaggcaa aatcgccgcc agcagcaacg cgccgccagc cagaccacaa gctaacatcc
    51421 cctgaagcgc cgaacgtttt gcgccatatt gatcggccag tcgcccggcg taaccacgcg
    51481 tcagcaccgt agccagaaac tgaatcccga cggcaatgcc gaccatggta ttgccatagc
    51541 ccagatcatg atgaacaaac agcgggataa ccggcaacgg caaccctacg gtcatgtagg
    51601 tgagaaaaac cgcaaaagag atgcggaaga gcgaaaaact ggcagaagat cgtgtttcgg
    51661 tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg agtatccccg
    51721 tctcatctct ctggtttcag ggttacggtg cgttggcagg atttaacgcg tacgtctttt
    51781 cagaaggaaa tcgacaaagc gggaagtttg cctagaactg gtggcgattg tcaatgatgt
    51841 gaaaaaggga accatcaggt tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa
    51901 cgtcggatgc gacgctggcg cgtcttatcc gacctactcc ggcctactgc gaattaatct
    51961 tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat
    52021 aacagtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc
    52081 gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga
    52141 gtagatagct tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg ctttggttgc
    52201 agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagct ctttcacgtc
    52261 gccaaccgga gtcgaagtac cgtgagagtt caggtaatcg attggggtat caacgccatg
    52321 catcgccatc tgcatacaac gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc
    52381 atcagaggtt gcgccgtagc caacgatttc agcatagatg taagcaccac gcgccagcgc
    52441 gtgttccagc tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg
    52501 gtgagcgtcg taagtacggg aggctttttc cggggtgtcg ttgtatttag tagatagcgc
    52561 acccatcgcg tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa
    52621 cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga
    52681 agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag
    52741 acaggcagaa acgccggatg ccatcgcttt agtgaccaca tacgggccaa ccgctttcag
    52801 gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc
    52861 ggaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggcccgc
    52921 atcagcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg
    52981 cacaactttg cggtcaatga ggccagtggt atccagtttt atgttgcccc agacgtggct
    53041 acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag
    53101 agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat
    53161 cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca
    53221 atagcgtaca cttgtacgcc gaacaagtcc gatcagccat ttaatagaga tatttgcgca
    53281 gccttacaca catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac
    53341 actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag
    53401 attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc
    53461 tgggaggtaa ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtgatag
    53521 cagagagcgg cttcggcacc ggattaaact tcctgacgct atggaaggca tttgatcagt
    53581 ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat
    53641 ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt
    53701 gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc
    53761 tcgatgaagg ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc
    53821 aactggacga ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag
    53881 ctaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc
    53941 caggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag
    54001 ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg
    54061 tgatggaaca gacattacca ctcccctgct ccacgccgtg gtttaatcgt acgggcagca
    54121 gcaaacggga agtagcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgt
    54181 tattacggcg cggctggcag gtagcgcttt attgcgcgga tgaagccccc gcgctgggtg
    54241 cttccggcaa tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa
    54301 accgcttttt ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta
    54361 aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata
    54421 aaatcgcaca gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa
    54481 atgcggttga acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag
    54541 gtggttggct gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg
    54601 gtttgcagat ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt
    54661 tgttgaattt tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc
    54721 atcaaatcag ccgtttcagc caaacgtcga ctctcccggt gtattcggtt gccgggcagg
    54781 tcagccatat tccgacaacg ccggaactgg cagagctgaa gcaggtgctg tgctatgacg
    54841 gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc
    54901 gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga
    54961 ttgattgttt cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc
    55021 gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt
    55081 atgaggcaac actcgtggaa tatgcgtcgt tggcggagca aaaagatgag gcggtaagcg
    55141 cgccggtttt tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg
    55201 ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg
    55261 ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta
    55321 aagcggttaa ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct
    55381 tatccaacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat
    55441 ggtgctccag acgccgcaac ttacagctgt gacgcctgct gaaacaggtt ttcccacata
    55501 tcggtcacca gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga
    55561 ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct
    55621 gcgatggcta atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg
    55681 gcatggtcaa ccatgccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc
    55741 ttctttcctc attgtaaaac gtggcgctga cttcagttga gcgcttccgg ccacgggaac
    55801 tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc
    55861 gggcgttccg gaatgccatt tgtcagataa tccatcacct gcgcatgtac ccgccgttga
    55921 aacaccacac gatccggttc gaaatcccct tccagattgt cgcaactgac gttaaacgga
    55981 tatccagccg ccacgcagaa taaccagtcg aacgcctgcg gcttcacttc aacatcttca
    56041 aactggcttt gcgtctgggc atcgcgtccg tccgggcaat accagtagcc gaaatcaacc
    56101 agctcacggc gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca
    56161 taaaagccat gagcaaagac gattcggtta tacggtactt ccgcatcagc aggaagatag
    56221 atcggttcgt cgtcgccttt aatcagacgg gtattaaatt catcggcaaa gcagctatta
    56281 aaaatttcaa tcaactgctc gtagtggtgt gtactgttca ttagttcatc cccaaccagt
    56341 ggaggatctc ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gccgagacga
    56401 taacaatcat cgggcggatc agcttttgtc ctttgctcaa caccaaccgt gaacccatgc
    56461 gcgcgccgag gaactgcccc accagcatga caaagcccgt cgcccaaatc actttgccgc
    56521 cgagaataaa cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat ttggcgtgag
    56581 ccgtggcttt ggcgaggttg aatccgcaca gcataacgaa ggccagcgcg taaaacgatc
    56641 cggcagccgg gccaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga
    56701 agggtaaacc gtacatccgg cgctggcgat cttcttcacc cagctttggc atcagcaaga
    56761 aatagaggcc gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa
    56821 cgtactgtac caacagtgcg ccgctcattg agccgacaaa ggtcatggca atattgagtt
    56881 tctgatcgct taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc
    56941 cgccgcaggc ttgcagttta ttggttgcca gcgcgttagc gggagacatt cccgcagcca
    57001 tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca
    57061 gcatggcgac aaaaaagaga actcccagca acagcggaga aaccataaac aggctattaa
    57121 acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg
    57181 cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag
    57241 ttctgccccg caaccatcgc ctgatggcgg taaaggttga tcttcacact ccagactatc
    57301 ggcagggcaa cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt
    57361 gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt
    57421 aacaaaaatg cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc
    57481 tggcttccac agcgtggaga caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg
    57541 cgggcgcaag agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac
    57601 atccagtccg gtctggtggc tggcatgacc gccgttgaaa cgcccaccag cgggcatccc
    57661 catatcgcca atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg
    57721 gataaacatc accagatccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg
    57781 ataatgttcg gactgtatcg gcaacgtgtc agcgccgaca atacagccat tagaaaaact
    57841 gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc
    57901 caggctagcg ctactggcaa gcagagccag cagcgcaatc gcggttttgt tcatttttta
    57961 ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc
    58021 cattaaaacg atcgccagca tcgcttcagc gatcggcact gcacggatcc cgacacaggg
    58081 atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc
    58141 cggcacggta atgctggagg tcggtttcag cgccatatga gcaatgattt gctgcccgct
    58201 gctgataccg ccgaaaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc
    58261 gtcgcggttc tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc
    58321 tttcaccgcg ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac
    58381 cggctcgcca agtccggcgg ggacaccact ggcaacaacg gtgactttcg cgccgatgga
    58441 gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc
    58501 cgggcagaag aacggatttt gttcaaccag cgaccagtct ttgatttcca gcggaatgtc
    58561 gcccatctgg gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt
    58621 ggcaatcgcc cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc
    58681 gcgataatcg cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc ctggacggaa
    58741 gacgtcttta attgcgctgt aatcctgaga acgctggtcg gtattttcga tcaacaagcc
    58801 aatgctggtg ccagtagtaa cgccttcaaa aacaccggag agtattttga cctgatccgg
    58861 ctcgcggcgc tgggtggtat agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg
    58921 caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc
    58981 cagcccgtgc gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc
    59041 catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat
    59101 gttctcgtgc ggcaataagc tgctctttag tgagcataaa cacaccatcg ccgccgttat
    59161 caaactccag ccaggtgaac ggaacatcgg gatattgttc cataagatgt accatgctgt
    59221 tgcccacttc acaaatcaac acgccatcat cagcaaggta atctgccgcg ttaccgagaa
    59281 tgcgacgcgt cagtttcagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac
    59341 ggtattcgtt tggcaggtcg gacatatctt ccgcgtcgac atacggcggg ttagtgacaa
    59401 tcagatcgta ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt
    59461 tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga
    59521 tatctaccgc gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc
    59581 cgctaccggt acacatatct aaaatatgct gcggttgctt gctgatgagt ccggcaaatt
    59641 tattgttgat cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa
    59701 attcatgacc gcagaaccag gctttgttgg tcaggtaagc caccggaatg cgctcgttga
    59761 cgcggcggat cacgcgttca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc
    59821 gcatatcttc cgggatatcc agcggcaggt agagcgatgg taacaccagc tgtacggctt
    59881 catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctta
    59941 ccgaccagcg caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa
    60001 ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg
    60061 aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat
    60121 cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa
    60181 aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac
    60241 tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt
    60301 gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct ccgatgagtt
    60361 tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt
    60421 tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg
    60481 tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct gtcgccgcga
    60541 acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac
    60601 accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac cgaaagagta
    60661 cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gaatggttgc cgccggagtt
    60721 gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg cctacaaata
    60781 tgtgataatt caataaattg caattcactt cgtaggcctg ataagacgca tcagagcaat
    60841 actacgaact tcagttcatt caatacatac tgaaaacgac acgtcgaatc aatcggttgc
    60901 ccactactgg tattcacatt caccacaata tcgtgtagta ctttatccgt taccggatga
    60961 cgagttggcg taaccgttac cgttgattta tcacaaccgt aagaggtaaa ggttaacaat
    61021 tctgataccg cgcggcagta tgctcctctg ccatcattat cagaaaaatt actgccatca
    61081 cagagtctgc cgccttccat caggtaggtc gaaagcgtgg tggtcactgt tgaccccgtg
    61141 gactgaattc gtaacgcctt attatggctg acataatttt cactccagga gatatcgtat
    61201 gaagcgaccg gtatcgttgg gcatattgtg ttatgcactg taggggccgt atagcttcca
    61261 aatccgggta aatctgtata ggaataaaag gatgcgatcc aagggcaacc ataatcatca
    61321 gatgcaacgg taattttaaa cgtcatatat gcacaacgaa aaattttcgt atctatgcca
    61381 gggcaatttg agttattaat tgtatcaaag tcagtcagta ccaaagttgg agctgaaccc
    61441 gacccagacc aggaaataac ccgcaacgta tcgcctttat acgcaaaaga atcatatttc
    61501 ccatatgcaa cctcctttgg cgttatcacc gtaacattga aaacactgct cgctacagca
    61561 ccggttacct tgtccatggc ataataaact ctcacactgt caaaactcgc actcataagt
    61621 ttatctccag cataactctt agatgcgccg acaacactca gaacgagcag cattaggctc
    61681 gcgaaaatta tggtccattt tttcatttca gaaatactca aaggttgccg tcactgtggc
    61741 actaaagtct ccggacgtta catctcgccc gctctttgcc tgtaaccagg tattaaaatt
    61801 taggctgtta tttccctgat tcagcgggag cgtgacgcct gttgtattgt tgattggcac
    61861 cgatgtgcct gccgcagttt caataccaat cccagcgccg gatgctgatg acgaggagtc
    61921 aaaggccaga aagccgggta gagcactatc ttctgttcct gtcaacgtta ctttgacatt
    61981 aaacaatgta gaactatgac aatctttcaa ttgaaacacc aatggcactc gctctgactg
    62041 cccaaggttc attaaatcac tggcggcaat ggcaggaaaa tgtacttctg ccaactggct
    62101 accattaatc actggcgtac acgatttact gattaaattg ccggtaaagt gcaaattatc
    62161 tcctgctatt gtggttccac tgcccagcaa catcaggcta ctaataaaaa acaacgtacg
    62221 ctttttcttc attggtaatc tacctgtaac gtggcccagg cctcaaagtc cgcctctggc
    62281 agaatgacac cactcttttt tactggcaca gccgttaaca ccggtaaatc ggtttcattc
    62341 accacaagtg gcgtattgat cgtaaaactc tgccctgcct gtttgagctg aatgcctaaa
    62401 ccagtgatat tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc actccagctc
    62461 aaggtcattt ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga atacggcacg
    62521 gtttgctcat aattgctgcc attaatgtca tcaataagca catcctgaaa agttatttca
    62581 atggtttgag cgttgctaat cgtgcagtta ggcggctgca ctaaataacc gtgaaaagag
    62641 acgtttgaga gtgccatcga gcaaaagccc cacagcaaaa taagcgagat ccgtttcatt
    62701 ggtaatccac cacaattgtg gcactggcgt taaattcagc tgccgctaac gttgtaccac
    62761 tctgtttcac cggcacagct tccagaacgg gaactcccga ggaaaaatta aatggcaacc
    62821 atgagccaga ggttaagtcg agtattgtgt gatcactact tttctgaacc cgaataccaa
    62881 acccaggaat acccgtgctg ataactgttt caccgttaat cacgaccgtt gtcgcctgca
    62941 tctgtagacg caggtcatcc attgccagat tgttacaaac aagcgagtag tcaatgggac
    63001 gtttataatc aaccctattg atcttattaa tgaatacatt accaaactca actgccgcac
    63061 catttaccgt acaaggagga ggggcatcga caagcacccg taaattcata cttactgact
    63121 tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca gttaccgccc
    63181 agacagaaca agatcctttc atactggctc tcccggcctc agcctttgcg gctctgctct
    63241 tcatcgacct agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg agcgccatag
    63301 tcgttgacat aggtcagtac cggcacgcta cccatcttca cattcagcgg tgcagaggtt
    63361 ttcggcgaca tcactattag cgagaaaccg gctgccggat tgccattttt ctgtgtgctg
    63421 gcattactga aaatgacgta atacgacgtc gggttattga cggtaaattc ctgaccgttg
    63481 cgggttaaag taatcttata ttgctagggg tttttcatgc tgacgttctc cagcgccttc
    63541 ggtcgccaga acaacttaat acgcgtttgt agtgctattt gcagcgtgtt ggctttgttt
    63601 gattttggcg ggatctcgcg cacgttgaag tagaacaggc tttcacgatc ggcaggcaat
    63661 ttgttgatat ccggcattcc ctgcactttt acctgcccgt tcatcattga atcgatgcgt
    63721 tgcaccggtg gcagaacagt taacggtgaa ctgatcttgt tacccttttc atcttcgatc
    63781 cagctttggg ccagataagg cagtttcgga tcgttattgc gcaacgtcac gctgattgat
    63841 ttgtcgcttt cattaaatat caaacgggta cgatctggcg ttacagaagc ctgggcagac
    63901 aaactggcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc agagcaaagt
    63961 aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag aaacaggggg
    64021 attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt catcaggaac
    64081 aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa acagatcggc
    64141 aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga acacactcat
    64201 gtgttcgcca ggtttcaccc cagccagata aacattgccg tcatcatcga caaggcccac
    64261 tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa
    64321 acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt agccaatcgc
    64381 cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt ccggcagttt
    64441 gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa cgacggcttt
    64501 accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat cggcaacgcc
    64561 atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc
    64621 gtgggcagta agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt attgcccttc
    64681 atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt aatagccgtc
    64741 aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg cgtcatcgac
    64801 acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagtacc gttatagcta
    64861 acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt gtcggcctgg
    64921 ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat actgcccata
    64981 ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc ccagtaggta
    65041 tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg gttataagtg
    65101 gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact ggcatccaga
    65161 tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa agtaacgcgg
    65221 ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc gtccagcgaa
    65281 cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg
    65341 accgcgccga atgtagacaa atcgcgaccg acaccaagcg ccgcagactg atagttttca
    65401 tctcccagtg cgccgccata taacgaccag ccgttagcga tcccccagga agcttcagca
    65461 ccagaaaaaa attcaccctc gacatggtgt ccccactctt gcggacggcc catcatgatc
    65521 ttatagcgaa cctgacctgg acgagtgagg tatggcatcg aggcggtgct gatgtcatat
    65581 tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga gacagaatca
    65641 ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc
    65701 atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag
    65761 ccgcgcagat tgggaggcaa catttgatcg tcagtactga cactgccacc aacatagtta
    65821 aaaccatcaa aaatatcgga tctgaggtaa tcctcgccca gcgccagttt ggcttttaat
    65881 gatggtaacg cccgcctggc atagtagcga ctccactccc attttttttg agtttcatcg
    65941 ccgctgaatt catcgtcatc attactgcga gtatgttgat agttagtctg ccagtcagca
    66001 cgcatacgcc acggccctag gttaacccca accgtcccgt tgccgctgat ctcgttacta
    66061 tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc
    66121 ccggagatgc cgtcatccca acgtgaaggc ggatcccagt cgggataagt atattcgagg
    66181 taagcctgtg gcagtgaaat gactaatgcg gactggctta aatcagcctt aatttccatg
    66241 ccttccagtt gaccggattt caggcattta gcatcgtggc tccattgcag atttttcgcc
    66301 acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg
    66361 ctcgcgtcat cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta
    66421 tttagttgaa cctgtaaatt gtattttccg ggctcaacat atccctagct ggaaaaacgc
    66481 ttcagatcaa tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg
    66541 tcttcagccc agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga
    66601 atccgtaatc gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt
    66661 tcggtcaaaa tacatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg
    66721 taagtaatct ggaaggtggt atttgcttca aaattaccca gatctggcgc atcagctgcg
    66781 ccgaccagcc aggcgttaaa gttcagtgtc tgcttcgctt tgcctttgtt ggtagaagtg
    66841 tctttcacta ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca
    66901 ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa
    66961 taattgccgc tatttgcaga agaaacggta ccagtaaagg tagtggtcat tgtttcctga
    67021 gtatcaaata cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta
    67081 tttttcagtt tttcggcacc gatatcaccc atatcaactt ccagtttgtg atcgtctgga
    67141 acgatagaac acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc
    67201 gcagcgattg ttgccgtaga ggtgaacacg cccatcacca ttgccgcagc aatagctgtt
    67261 ttaacaaact tactcattat ttgtccttaa actataaata attaaaaata taaacattgc
    67321 aatacattga ttcagtcaat agccaatgtt tatacccagc cattgaatac ggcatgcgac
    67381 ccaaaaagaa caggtcgtag aaaattgtat tcattggtgt ggggccgcat agtgatgttt
    67441 ccacacccct gatccaattt aattttacta aagagtaaat gtagatttaa ttaatatatt
    67501 gacagaagaa aggttatctt aaggacatcc ttaaaaaata cggaataagt atttagaaaa
    67561 aatagaaagt atatcaataa tgaatatcaa cagcttaaaa aacaaacaat gaacatataa
    67621 tgcgattggc atttatccac ttttgacaat ataatatata aacactatag ccagaattta
    67681 atcaagtttc aattacatcc tgcctgatta ttcaactcta ttttctctat tatctgtatt
    67741 aataaatgac aatgagtgtc aaacagatat tattcatttc aacatgtaac tttgggaaaa
    67801 atcaagatac gcgcagaaga ataatttccg aatgaatgta gaaagtgttt tatttataga
    67861 taaggagagt cttcgatagt agtgaggttg acagatatcc gctgcctaat gagcaattat
    67921 gcccaatgaa tctacctcat tgggcataat ttggaacaca tcagatagct tttgccatct
    67981 tcagattaca cgggctcatc tgccagttga atgtaccgtt tccgctttca tcaagcgtta
    68041 cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat aactcggcaa
    68101 ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca ccttcattgg
    68161 tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt aactccggca
    68221 gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt gtttgctcgg
    68281 ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct ttcagccagt
    68341 tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacc gaatcactgg
    68401 cggaatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg caccgctttt
    68461 gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc
    68521 attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg cgtaagtata
    68581 gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt catatagctt
    68641 tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc acgcgcgccc
    68701 atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc cagtcgttgc
    68761 attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg tccaccgaga
    68821 aatggcggaa aaccaatgcc aaataccgca ccaatatccc cgtcacgcac gctacggata
    68881 acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca ccgttcagca
    68941 acctgcggtg cggagagtca cccctgccct tgtgcgccaa tgagcgggta aatggcagga
    69001 tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc ccgaccattt
    69061 tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa
    69121 cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc gattcctacc
    69181 tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcactagcgc ggcatcaatg
    69241 tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta aggcgctaag
    69301 atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt
    69361 ttcgccagtt ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg aggaataatc
    69421 tctaccagcg gcattttttc caccggactg aagaaatgca ggccaataac ttgctcaggt
    69481 cgcgcggcgt gagcggcgat atcaccaatc ggtaaagatg acgtgttcga agcaaaaatg
    69541 gtatgagcgg cgcaattttg ctccacttcc gccaccatct gttgttttaa ttcgagattt
    69601 tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg
    69661 gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg gcgaactttg
    69721 ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg gttgatatct
    69781 ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc gccgcccatc
    69841 aagccaccac ctaaaattcc cacgctgttt aatgccgcag gcggtgcatc actaccggga
    69901 tctttcttca cgtccgtact ggcaaaaaag atactacgca aagcttgcga ttgcggcgtc
    69961 atcgccagtt cgccaaaggc gcgagcttca gcgtcatagc cgctgctggt gccctgcgct
    70021 aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc ttgagttttg
    70081 tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg ccccgccaga
    70141 atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc cagctcaaca
    70201 gcggcttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag ctttaatgcc
    70261 tgtttcgccc gaagttgttt tccggtgaga atcatctcta atgctgtact gacgcctatc
    70321 agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg tacttcagac
    70381 aaaccgagca ccgttttagg atcgtcagta caaacgcgac cgtggcacgc cagcgccagc
    70441 tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaat gggcaaagca
    70501 tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt
    70561 ttgcagttgc caatcatgtt gatgtctgct ccggcgataa aattgtccgg tttcgcggag
    70621 ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat ggcgcgcacc
    70681 tgcgaggcaa attccgcctt cagggtattc attttctcat ccggtacgtc gatggtgatg
    70741 atagcaatgt tgtccagacg aacattaaga gtaaacgctg atgccatttc cattattccg
    70801 cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact aaaccaaatc
    70861 caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg
    70921 cgaagggatg tccgtaagca atcgaaccgc caagcacgtt aaatttgcta tcgtccactt
    70981 cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta cccagcaact
    71041 gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat gtcagttcgc
    71101 tcatcgtcaa accggcacgt tccagcgcca gcggtgttga ccaggctgga ccgagcaaca
    71161 tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc agcggcacca
    71221 gccctaattc tttcgcccgg gattcagtca tcaggatcac cgctgccgcg ccatcggtca
    71281 gcggcgtact gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct
    71341 ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca agcggttgtt
    71401 tataaggagg gataaaggca gtcatcacct cttctttgag ttttccgtct gaccatgcct
    71461 gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt
    71521 aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta
    71581 ccgcaggcgg tacgggcatt aagtcgcgca aacgcaggcg agagaagagt ttcaggcgct
    71641 ggctcatggt tcgagctttg ttgacatcaa ccagcacgtg cgccagtttt ttactgacgc
    71701 caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg atagttcccg
    71761 ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga
    71821 cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca cgcgcaatgt
    71881 tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc acttcagcgg
    71941 ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca accgtgggaa
    72001 tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta acaatggcga
    72061 tacgatcgcc ctggcgggta accagtggta aaacctgacc cataacactc ccctgtaaaa
    72121 aaataaataa agtggtctga cctgatcata gtcttaacca tttttttaca tttagccaag
    72181 cggagaaaaa ggaaagtggg agctatgaca cagtgaagac agaagaaaga aaagaaaacg
    72241 cccctgccag caactgacag gggcgtacgt acccgagagg aattaacgca gacccagctg
    72301 gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc
    72361 agactcttca gtgaaagtcg gggagatttt gcacggttcg gattccacgc tacgggattt
    72421 ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca cgcggctgta
    72481 agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg
    72541 ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc
    72601 cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg
    72661 caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga
    72721 atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta gatcatattt
    72781 gaaaaaagat ggaaacatac ttgcaacatt ctagctggtc cgacctatac tctcgccact
    72841 ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa
    72901 catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta
    72961 caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct
    73021 ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa
    73081 ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg tttgaccgcc
    73141 cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc ggaacgtctc
    73201 cgtctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc
    73261 actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc tctaactatg
    73321 gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt acaactgacc
    73381 ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca tggagctttg
    73441 gtcttggttt caacgccatc tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg
    73501 ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct caagggcaag
    73561 cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat ctgaacggta
    73621 accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa aataaccgct
    73681 atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc
    73741 ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt ggcgcaacgc
    73801 aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt tataaccgtg
    73861 ttgatccaca gtgggcgatt cactatagcc tggcttacac cagctggagt cagttccagc
    73921 agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa ggctttaaag
    73981 atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg accttccgta
    74041 ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc tccattccgg
    74101 accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa gatgcttcag
    74161 tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa ggcccatacc
    74221 agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat
    74281 aacgcgttcg cctagataaa gtcacctgct atgcaggtga ctttaactcc cccacttcac
    74341 cgaaagtagt ttcctcattg cttgcctcct gagttttgca aacagcctga tggcagcttg
    74401 ccctttcaaa tcagtaagta gtatccacag acaaacctga agggaaatgc atattttcaa
    74461 gcgttctgct cgtccttctc aaagagttta cttttctgca tttccaggat actcaacccc
    74521 cctggctatt gtgcgcttat acactcaaat taaagatagg ttctaaataa atgagcgttt
    74581 tttgatagtc tatttcatta ggtaatatat atttgtaaca aatcaatcaa catggaataa
    74641 aatcatgcta ccatctattt caatcaacaa taccagcgca gcttacccag aatccatcaa
    74701 tgaaaataac aatgatgaaa ttaatggatt agtacaagag ttcaaaaacc atttttaatg
    74761 gtaaggaagg aataagcacc tgtattaaac atctacttga gcttataaaa aacgccatac
    74821 gagtaaacga cgatccttat agatctaata ttaataattc ctcagttact tatattgata
    74881 ttggctccaa tgatacagac catattacta ttggtatcga caaccaagaa ccaatagaat
    74941 tacctgcgaa ctataaagac aaagaactcg tccgtactat cattaatgac aacatagttg
    75001 agaagactca tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa gaaatatatg
    75061 atggagatcc tggttttatc ttcgataaga tatcacacaa actcagacat acggtaacgg
    75121 aatttgatga gagcgggaaa agcgaaccaa cggacttatt tacctggtac ggtaaagata
    75181 aaaaaggcga ctctctcgct attgtaatta aaaataaaaa cggaaatgat tacttatttc
    75241 tcggttacta cgatcaggac gactaccaca ttcaaagagg aattcgtatt aatggtgata
    75301 gtctcaccca atattgtagt gaaaacgcca ggagtgcttc agcgtggttt gaaagcagta
    75361 aagctatcat ggcagaatca tttgcaactg gttccgatca tcaggttgta aacgagctca
    75421 acggggaaag actgagagaa cctaacgaag tttttaaacg tttaggtcga gcaataagat
    75481 ataattttca agtggacgat gcaaaattta gacgcgataa tgtaaaagaa ataatttcta
    75541 ctttattcgc taacaaagtt gacgttgacc atcctgaaaa taaatacaaa gactttaaga
    75601 atctcgaaga taaagttgaa aaaagacttc aaaatcgcca gacgaaatat caaaatgaaa
    75661 ttaatcaact atctgcacta ggtgttaatt ttgatgatat ttaaaattat ttttccagtt
    75721 ttttgatttt tcaacgcctg aatatcggaa atagtcagtg acaaacatca gtatttttac
    75781 atcattacgt ccgggtcatt gtcggtttat ctccttttac ttgtggtaat tgagggataa
    75841 caacaaaaaa ggtgagtttt gcgactcacc ttttttattt gtttcttatt cagaatcgat
    75901 atcttttaaa tcatcctgaa tcgcttgcgc gttcggattt tcctgcggtt tgagttcgcc
    75961 gccattagcg atgaaatcat gacgctggaa gtacgcttcg cgcaccataa tataaggatc
    76021 ggacgactga cgcagcagac catcggaatc cagcagctga gcgcgggttt cgatcccttc
    76081 aagcgtccat ttacccacag acatcggcca ggtcagccag gaaagaaccg ggtaaagagc
    76141 atccgccata tcaccaccgt catcacgcag cgtgaagcta ccgtagaacg gtaactgaac
    76201 gtaaggccca taacccacgc cataatgacc aagcgtacta ccgaagcggt gaggttcagt
    76261 ccgttgcagt ttcgggttcg ccatccctgc aacatcaata aagccgccca tccccaaaat
    76321 ggtgttcagg aaaaagcggg taaagtggac catcccctga taagggtcgc cctgcaagaa
    76381 gtagttaacc atcaccgcag gttcttcaag gttgccagta aagttgctca aaccattacg
    76441 cgccggttgc ggaacataat cacgccaggc gacagcgacc ggtcgaacaa tatacgggtc
    76501 taatacgttg aagttgaagt tgtacatggt gcggttgaac ccttctaacg ggtcagaacg
    76561 cccttgctga tctgtaccgg aactcgcaca ccccaccaga agcgtagttc ccagagcaag
    76621 cgccgacagg cgaagcttca taaatgtctc cctgtttttt tatggcttat gcagtttgcc
    76681 atccatgacg gaacgatacc gtatccgcct gtttaggtgt gggcgattgt aacagcacgt
    76741 caactgatgt ccagacgccc tgatttgctg atttgatcat agcctggtaa tcgccgccct
    76801 gtaggctact tgattctata gaaacagaaa aaggcaaacg cagccttttc tacattttca
    76861 gagtaactcc cgccagttgc gagcaaaaaa gccgctacgc tttagctata cgtgctaatt
    76921 caagagaaga gaccatggac aacgacaaaa ttgatcaaca cagcgacgaa attgaagttg
    76981 agagcgaaga aaaagagcgc ggcaaaaaaa tagaaataga tgaagaccga ctcccctccc
    77041 gagcgatggc aattcatgag catatccgcc aggatggtga aaaagagcta gaacgcgacg
    77101 caatggcgct actgtggtca gccattgcgg cgggtctgtc gatgggcgct tcgttactgg
    77161 caaaagggat atttcatgcc gaactggaag gtgtgccggg cagcttcttg ctggagaatc
    77221 tcggttatac ctttggtttt attatcgtca taatggcccg ccagcaatta tttaccgaaa
    77281 acaccgtgac tgcggtacta cccgtcatgc aaaaaccgac aatgagcaac gttggcttac
    77341 ttatgcggtt atggggagtc gtgctgctgg gtaatattct cgggactggt attgctgcgt
    77401 gggcatttga atatatgcct atcttcaatg aagaaactcg cgatgcattt gtcaaaatcg
    77461 gcatggatgt gatgaagaac acccccagcg agatgtttgc caacgcgatc atttccggct
    77521 ggctgatcgc cactatggta tggatgtttc ctgcagcagg tgcggcaaag attgtggtga
    77581 ttatattgat gacctggctt attgccctgg gtgacaccac ccatatcgtg gtcggttctg
    77641 ttgaaatcct ctatctggtg tttaacggta cgctgcactg gagcgatttc atctggccct
    77701 tcgcactacc tactttagcg gggaacatct gcggcggcac ctttatcttc gcgttaatga
    77761 gtcatgcaca gattcgtaac gacatgagca acaagcgtaa agcagaagca cgccaaaaag
    77821 cagaacgtgc ggaaaacatt aagaaaaatg ataaaaaccc agcataaatg gcgagggttt
    77881 aagcaatcga gcggcagcgt acttaccccg cagtccatta gcgggtatac tcgccgcatt
    77941 gtcctcttag ttaaatggat ataacgagcc cctcctaagg gctaattgca ggttcgattc
    78001 ctgcagggga caccatttat cagttcgctc ccatccgtac cagtccgcaa aatcccctga
    78061 atatcaagcc ttccgtatat tcacagttcg tcatggttcg cgtcagatcg ttgacagccg
    78121 cactccatga cgggtaaaaa gtggataaaa taattttacc caccggattt ttacccatgc
    78181 tcaccgttaa gcagattgaa gcagcaaagc cgaaagaaaa accataccgc cttctcgatg
    78241 gtaatggcct gtacctttat gtccctgtat ccgggaaaaa ggtatggcag cttcgctaca
    78301 agattgacgg taaggagaaa atcctgaccg tcggaaaata tccgcttatg actttgcagg
    78361 aggcaaggga taaagcatgg actgcgagga aagacatctc ggctggcatc gatccggtaa
    78421 aggcgaaaaa ggcttcgtct aacaacaatt catttagtgc gatttacaag gaatggtacg
    78481 aacacaagaa gcaagtctgg tcagtagggt atgcgactga acttgcaaaa atgtttgatg
    78541 acgacatttt acccatcatc ggcggccttg agattcagga tattgagccg atgcaactgc
    78601 tggaagtaat ccgcagattt gaagatcgcg gtg
//
LOCUS       DBKELA010000002        75858 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.002, whole genome shotgun sequence.
ACCESSION   DBKELA010000002 DBKELA010000000
VERSION     DBKELA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.002"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..208)
                     /locus_tag="ACUQR0_000077"
     CDS             complement(<1..208)
                     /locus_tag="ACUQR0_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107295.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDR"
     gene            complement(210..430)
                     /locus_tag="ACUQR0_000078"
                     /pseudo
     CDS             complement(210..430)
                     /locus_tag="ACUQR0_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(423..644)
                     /locus_tag="ACUQR0_000079"
     CDS             complement(423..644)
                     /locus_tag="ACUQR0_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107296.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(634..834)
                     /locus_tag="ACUQR0_000080"
     CDS             complement(634..834)
                     /locus_tag="ACUQR0_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HHV9107297.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            964..1143
                     /locus_tag="ACUQR0_000081"
     CDS             964..1143
                     /locus_tag="ACUQR0_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107298.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1268..1597
                     /locus_tag="ACUQR0_000082"
     CDS             1268..1597
                     /locus_tag="ACUQR0_000082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107299.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1647..1835)
                     /locus_tag="ACUQR0_000083"
     CDS             complement(1647..1835)
                     /locus_tag="ACUQR0_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9107300.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2210..3438)
                     /locus_tag="ACUQR0_000084"
                     /pseudo
     CDS             complement(2210..3438)
                     /locus_tag="ACUQR0_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3687..4808
                     /gene="roxA"
                     /locus_tag="ACUQR0_000085"
                     /gene_synonym="ycfD"
     CDS             3687..4808
                     /gene="roxA"
                     /locus_tag="ACUQR0_000085"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HHV9107301.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="ACUQR0_000086"
     CDS             complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="ACUQR0_000086"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HHV9107302.1"
                     /translation="MDKLLERFLNYVFLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6333..7469
                     /gene="potA"
                     /locus_tag="ACUQR0_000087"
     CDS             6333..7469
                     /gene="potA"
                     /locus_tag="ACUQR0_000087"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HHV9107303.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7453..8310
                     /gene="potB"
                     /locus_tag="ACUQR0_000088"
     CDS             7453..8310
                     /gene="potB"
                     /locus_tag="ACUQR0_000088"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HHV9107304.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8307..9101
                     /gene="potC"
                     /locus_tag="ACUQR0_000089"
     CDS             8307..9101
                     /gene="potC"
                     /locus_tag="ACUQR0_000089"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HHV9107305.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9098..10144
                     /gene="potD"
                     /locus_tag="ACUQR0_000090"
     CDS             9098..10144
                     /gene="potD"
                     /locus_tag="ACUQR0_000090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HHV9107306.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="ACUQR0_000091"
     CDS             complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="ACUQR0_000091"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HHV9107307.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="ACUQR0_000092"
     CDS             complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="ACUQR0_000092"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HHV9107308.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="ACUQR0_000093"
     CDS             complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="ACUQR0_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HHV9107309.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="ACUQR0_000094"
     CDS             complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="ACUQR0_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HHV9107310.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="ACUQR0_000095"
     CDS             complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="ACUQR0_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HHV9107311.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15476..16549
                     /locus_tag="ACUQR0_000096"
     CDS             15476..16549
                     /locus_tag="ACUQR0_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHV9107312.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16677..20123
                     /gene="mfd"
                     /locus_tag="ACUQR0_000097"
     CDS             16677..20123
                     /gene="mfd"
                     /locus_tag="ACUQR0_000097"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HHV9107313.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20266..21228
                     /gene="ldtC"
                     /locus_tag="ACUQR0_000098"
     CDS             20266..21228
                     /gene="ldtC"
                     /locus_tag="ACUQR0_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HHV9107314.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21311..21567)
                     /gene="bhsA"
                     /locus_tag="ACUQR0_000099"
                     /pseudo
     CDS             complement(21311..21567)
                     /gene="bhsA"
                     /locus_tag="ACUQR0_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
     gene            21808..22440
                     /gene="comR"
                     /locus_tag="ACUQR0_000100"
     CDS             21808..22440
                     /gene="comR"
                     /locus_tag="ACUQR0_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HHV9107315.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22502..23041)
                     /locus_tag="ACUQR0_000101"
     CDS             complement(22502..23041)
                     /locus_tag="ACUQR0_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HHV9107316.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23268..24572)
                     /gene="ndh"
                     /locus_tag="ACUQR0_000102"
     CDS             complement(23268..24572)
                     /gene="ndh"
                     /locus_tag="ACUQR0_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HHV9107317.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24980..25522)
                     /gene="ycfP"
                     /locus_tag="ACUQR0_000103"
     CDS             complement(24980..25522)
                     /gene="ycfP"
                     /locus_tag="ACUQR0_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HHV9107318.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="ACUQR0_000104"
     CDS             complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="ACUQR0_000104"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HHV9107319.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="ACUQR0_000105"
     CDS             complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="ACUQR0_000105"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HHV9107320.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="ACUQR0_000106"
     CDS             complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="ACUQR0_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HHV9107321.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28042..28419)
                     /locus_tag="ACUQR0_000107"
     CDS             complement(28042..28419)
                     /locus_tag="ACUQR0_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HHV9107322.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="ACUQR0_000108"
     CDS             complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="ACUQR0_000108"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HHV9107323.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29120..31305
                     /gene="fhuE"
                     /locus_tag="ACUQR0_000109"
                     /pseudo
     CDS             29120..31305
                     /gene="fhuE"
                     /locus_tag="ACUQR0_000109"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31365..32798)
                     /gene="ptsG"
                     /locus_tag="ACUQR0_000110"
     CDS             complement(31365..32798)
                     /gene="ptsG"
                     /locus_tag="ACUQR0_000110"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0055056 - D-glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - D-glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HHV9107324.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33093..33890)
                     /locus_tag="ACUQR0_000111"
     CDS             complement(33093..33890)
                     /locus_tag="ACUQR0_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9107325.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33901..34905)
                     /gene="holB"
                     /locus_tag="ACUQR0_000112"
     CDS             complement(33901..34905)
                     /gene="holB"
                     /locus_tag="ACUQR0_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HHV9107326.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34902..35543)
                     /gene="tmk"
                     /locus_tag="ACUQR0_000113"
     CDS             complement(34902..35543)
                     /gene="tmk"
                     /locus_tag="ACUQR0_000113"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHV9107327.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35533..36555)
                     /gene="yceG"
                     /locus_tag="ACUQR0_000114"
     CDS             complement(35533..36555)
                     /gene="yceG"
                     /locus_tag="ACUQR0_000114"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HHV9107328.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36558..37367)
                     /gene="pabC"
                     /locus_tag="ACUQR0_000115"
     CDS             complement(36558..37367)
                     /gene="pabC"
                     /locus_tag="ACUQR0_000115"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HHV9107329.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37487..38728)
                     /gene="fabF"
                     /locus_tag="ACUQR0_000116"
     CDS             complement(37487..38728)
                     /gene="fabF"
                     /locus_tag="ACUQR0_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HHV9107330.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38815..39051)
                     /gene="acpP"
                     /locus_tag="ACUQR0_000117"
     CDS             complement(38815..39051)
                     /gene="acpP"
                     /locus_tag="ACUQR0_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHV9107331.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39262..39996)
                     /gene="fabG"
                     /locus_tag="ACUQR0_000118"
     CDS             complement(39262..39996)
                     /gene="fabG"
                     /locus_tag="ACUQR0_000118"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HHV9107332.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40009..40938)
                     /gene="fabD"
                     /locus_tag="ACUQR0_000119"
     CDS             complement(40009..40938)
                     /gene="fabD"
                     /locus_tag="ACUQR0_000119"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HHV9107333.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40954..41907)
                     /gene="fabH"
                     /locus_tag="ACUQR0_000120"
     CDS             complement(40954..41907)
                     /gene="fabH"
                     /locus_tag="ACUQR0_000120"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HHV9107334.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41975..43045)
                     /gene="plsX"
                     /locus_tag="ACUQR0_000121"
     CDS             complement(41975..43045)
                     /gene="plsX"
                     /locus_tag="ACUQR0_000121"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HHV9107335.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43126..43299)
                     /gene="rpmF"
                     /locus_tag="ACUQR0_000122"
     CDS             complement(43126..43299)
                     /gene="rpmF"
                     /locus_tag="ACUQR0_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HHV9107336.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43351..43872)
                     /gene="yceD"
                     /locus_tag="ACUQR0_000123"
     CDS             complement(43351..43872)
                     /gene="yceD"
                     /locus_tag="ACUQR0_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HHV9107337.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44071..44655
                     /gene="yceF"
                     /locus_tag="ACUQR0_000124"
     CDS             44071..44655
                     /gene="yceF"
                     /locus_tag="ACUQR0_000124"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HHV9107338.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44767..45726)
                     /gene="rluC"
                     /locus_tag="ACUQR0_000125"
     CDS             complement(44767..45726)
                     /gene="rluC"
                     /locus_tag="ACUQR0_000125"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HHV9107339.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46299..49484
                     /gene="rne"
                     /locus_tag="ACUQR0_000126"
     CDS             46299..49484
                     /gene="rne"
                     /locus_tag="ACUQR0_000126"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HHV9107340.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49680..50633)
                     /gene="flgL"
                     /locus_tag="ACUQR0_000127"
     CDS             complement(49680..50633)
                     /gene="flgL"
                     /locus_tag="ACUQR0_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HHV9107341.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50645..52287)
                     /gene="flgK"
                     /locus_tag="ACUQR0_000128"
                     /pseudo
     CDS             complement(50645..52287)
                     /gene="flgK"
                     /locus_tag="ACUQR0_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52353..53294)
                     /gene="flgJ"
                     /locus_tag="ACUQR0_000129"
     CDS             complement(52353..53294)
                     /gene="flgJ"
                     /locus_tag="ACUQR0_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HHV9107342.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53294..54391)
                     /gene="flgI"
                     /locus_tag="ACUQR0_000130"
     CDS             complement(53294..54391)
                     /gene="flgI"
                     /locus_tag="ACUQR0_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HHV9107343.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54403..55101)
                     /gene="flgH"
                     /locus_tag="ACUQR0_000131"
     CDS             complement(54403..55101)
                     /gene="flgH"
                     /locus_tag="ACUQR0_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HHV9107344.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55154..55936)
                     /gene="flgG"
                     /locus_tag="ACUQR0_000132"
     CDS             complement(55154..55936)
                     /gene="flgG"
                     /locus_tag="ACUQR0_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HHV9107345.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56074..56661)
                     /gene="flgF"
                     /locus_tag="ACUQR0_000133"
                     /pseudo
     CDS             complement(56074..56661)
                     /gene="flgF"
                     /locus_tag="ACUQR0_000133"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(56763..56825)
                     /locus_tag="ACUQR0_000134"
                     /pseudo
     CDS             complement(56763..56825)
                     /locus_tag="ACUQR0_000134"
                     /inference="COORDINATES: protein motif:HMM:NF012674.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(56845..58053)
                     /gene="flgE"
                     /locus_tag="ACUQR0_000135"
     CDS             complement(56845..58053)
                     /gene="flgE"
                     /locus_tag="ACUQR0_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HHV9107346.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(58078..58773)
                     /gene="flgD"
                     /locus_tag="ACUQR0_000136"
     CDS             complement(58078..58773)
                     /gene="flgD"
                     /locus_tag="ACUQR0_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HHV9107347.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58785..59188)
                     /gene="flgC"
                     /locus_tag="ACUQR0_000137"
                     /pseudo
     CDS             complement(58785..59188)
                     /gene="flgC"
                     /locus_tag="ACUQR0_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59192..59608)
                     /gene="flgB"
                     /locus_tag="ACUQR0_000138"
     CDS             complement(59192..59608)
                     /gene="flgB"
                     /locus_tag="ACUQR0_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HHV9107348.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59763..60422
                     /gene="flgA"
                     /locus_tag="ACUQR0_000139"
     CDS             59763..60422
                     /gene="flgA"
                     /locus_tag="ACUQR0_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HHV9107349.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60498..60791
                     /gene="flgM"
                     /locus_tag="ACUQR0_000140"
     CDS             60498..60791
                     /gene="flgM"
                     /locus_tag="ACUQR0_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HHV9107350.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60796..61212
                     /gene="flgN"
                     /locus_tag="ACUQR0_000141"
     CDS             60796..61212
                     /gene="flgN"
                     /locus_tag="ACUQR0_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HHV9107351.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61252..62787)
                     /gene="murJ"
                     /locus_tag="ACUQR0_000142"
     CDS             complement(61252..62787)
                     /gene="murJ"
                     /locus_tag="ACUQR0_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HHV9107352.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62954..63820)
                     /locus_tag="ACUQR0_000143"
     CDS             complement(62954..63820)
                     /locus_tag="ACUQR0_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHV9107353.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63822..64469)
                     /locus_tag="ACUQR0_000144"
                     /pseudo
     CDS             complement(63822..64469)
                     /locus_tag="ACUQR0_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64480..65064)
                     /gene="rimJ"
                     /locus_tag="ACUQR0_000145"
     CDS             complement(64480..65064)
                     /gene="rimJ"
                     /locus_tag="ACUQR0_000145"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HHV9107354.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65300..66508
                     /gene="mdtH"
                     /locus_tag="ACUQR0_000146"
     CDS             65300..66508
                     /gene="mdtH"
                     /locus_tag="ACUQR0_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HHV9107355.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66572..67219
                     /gene="grxB"
                     /locus_tag="ACUQR0_000147"
     CDS             66572..67219
                     /gene="grxB"
                     /locus_tag="ACUQR0_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HHV9107356.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67353..67913
                     /locus_tag="ACUQR0_000148"
     CDS             67353..67913
                     /locus_tag="ACUQR0_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HHV9107357.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68019..69065
                     /gene="pyrC"
                     /locus_tag="ACUQR0_000149"
     CDS             68019..69065
                     /gene="pyrC"
                     /locus_tag="ACUQR0_000149"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HHV9107358.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69139..69384
                     /gene="dinI"
                     /locus_tag="ACUQR0_000150"
     CDS             69139..69384
                     /gene="dinI"
                     /locus_tag="ACUQR0_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HHV9107359.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69674..69928
                     /gene="bssS"
                     /locus_tag="ACUQR0_000151"
     CDS             69674..69928
                     /gene="bssS"
                     /locus_tag="ACUQR0_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HHV9107360.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70043..71161
                     /gene="solA"
                     /locus_tag="ACUQR0_000152"
     CDS             70043..71161
                     /gene="solA"
                     /locus_tag="ACUQR0_000152"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HHV9107361.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71209..71322
                     /locus_tag="ACUQR0_000153"
     CDS             71209..71322
                     /locus_tag="ACUQR0_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HHV9107362.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71583..72149
                     /locus_tag="ACUQR0_000154"
     CDS             71583..72149
                     /locus_tag="ACUQR0_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HHV9107363.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72153..72728
                     /locus_tag="ACUQR0_000155"
     CDS             72153..72728
                     /locus_tag="ACUQR0_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HHV9107364.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72770..73822)
                     /locus_tag="ACUQR0_000156"
     CDS             complement(72770..73822)
                     /locus_tag="ACUQR0_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HHV9107365.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74047..74967
                     /gene="lpxL"
                     /locus_tag="ACUQR0_000157"
     CDS             74047..74967
                     /gene="lpxL"
                     /locus_tag="ACUQR0_000157"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HHV9107366.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75139..75324
                     /locus_tag="ACUQR0_000158"
                     /pseudo
     CDS             75139..75324
                     /locus_tag="ACUQR0_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            75360..>75858
                     /locus_tag="ACUQR0_000159"
     CDS             75360..>75858
                     /locus_tag="ACUQR0_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHV9107367.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEG"
BASE COUNT        18495 a        20066 c        18759 g        18538 t
ORIGIN      
        1 atctgtcgtg caatagatct atttcttgca gttccattat tacaggctca agaagcgtta
       61 ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc attgtcgcca agtgtttcaa
      121 gccatatgcg caatatttcc agcatgtttt cactgtgaga aagtgcaaga aatgcgcggt
      181 ctattgtttc gtggtaaata tcacgcatat taatccccgt ccgtcgtttt tcttaaaata
      241 acctctgtca cgaaatcagc ataatcggcg gcgatattca gtacatcaag cccgttgatt
      301 tatattctct cgtggaaagt aagaaaaaag ccgctctaat aagttctggc attgacgaaa
      361 gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg tttcagggaa ttaaaacgat
      421 catcacgcat aacccccccc attttcacaa tcggcaacaa gaatattttt agcgtcattc
      481 agtgaccgcg ttgcgatgta tcggatacat tccagggcga actgtgtccg gtctttctgt
      541 tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa taagcgcgtg catcagcgta
      601 gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt gcataggttt ccccctgacg
      661 aatacgggcg gcgaatacag caacacaacc ggacgggcaa cggctacgcg cttcgcgttc
      721 cgtccaagcg gttacgtgga tgatttggga ttctccggca ctcagtgcca gaaaacgcca
      781 cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga tttacggcaa ccataacggc
      841 tcctagttta cgttgttggt tagaagcccc gttactgctc ctacagtgcg gggctttgct
      901 ttttgtgttg attgtgtgta atgtgtcact acacataaat acattacctg aggtgtaatt
      961 aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc cgttccagtt ccgtttagat
     1021 cctgacttac gggaaaagat ggaaaaggca caacaacagg acggcgatga ttctcttgct
     1081 gcctggataa agcgaataat ccgcaaagaa ttacagcaac gcggcataga acccaaagga
     1141 taaaaactat cagcgccgtg gtgtgaggta ctacggcgca ttgctttaca ggcgcacaca
     1201 atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac gaaaaagacg acgcagaaag
     1261 aaagagcatg aatcagaacg attcgcgccg tgttcttttg cccttgagaa gtaccttaaa
     1321 gaatactcat caaaggagag aacttcgcag ttatggcaac gcactgaacc agactgattg
     1381 cattgcccac cagcctgata gcggctatca tccccgtgct tatgtttggg atcacacaca
     1441 caaacggcgc agcgggttat ctgttcagaa aggcggctcc atttcggggc cgcttttttt
     1501 atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac cagggcgaac gaatccccgc
     1561 ccacgttctg gcgtatattc aatcttcatg gttatagctc tgtgttcaga tgattgatgt
     1621 gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct tgcagctatc cttccaggcc
     1681 agaacctcgg ataaagacca gccaacggaa cgaccgccaa gtttacgacg tgatgggaat
     1741 tgtccggcct tttccaggcg gtagcggcat gagcggctaa ggcctgttag cttttcgcat
     1801 tctttttcac gtataaaccg atcagtgctt aacactattg ccccctttcg tttcttaaag
     1861 agttatttcg tgttctattg cgttgggatg tgtctgattg tgtcaggatg attcagagtt
     1921 ggcaaatgtt gaggccgcat ggtttacaga aagaggaata atcaggataa aatcctttaa
     1981 attcatgtta atacaaaggc ataaaatatt gtttcatgcc ttttttctcg ctatttaaag
     2041 agtgattcgc tagtgtataa aaaatcagca atacattaaa aatcagctat ttataaatct
     2101 gtacactttt tcacctcttg ttcgtagttg ttccgctttg ttgctcattg ttgcacgttg
     2161 tatctgttcg catatccagt atgcgcatac tgaaaaaaca cgaaaaaaat tattttcttc
     2221 tggctactgg tagcgtggtt acgttttcat gtgttcccgc cagtatccct aaccgctccg
     2281 tccacatatc cagcgcattg cgtttagcat ccagataacg ggaatgatta taaactcgct
     2341 gcattcctgg catctggtgg cctgtaagct gctccacgac gtgaggatcc acgcctaaat
     2401 cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag tgaccagtga gggtgattta
     2461 gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt ttcctgtttc acttccccca
     2521 gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt gacgaacggc agtattgctt
     2581 ccggtatggg ccggaatatt gccaccttcg ttttgctgtg ttctttcggc acggtccaga
     2641 gcatttcggt aaaatcccac tcgctgatct ccgataacct cagttctacc gtccggcatc
     2701 cgaacacaat caggaggcgg attaacgcga tgtagtaggg ggagaatatt tttttgtcca
     2761 gtgcctgcaa taattcgccc agttctttgg tgcttaagac acgctcgctt atatccggtt
     2821 ttttcccaac gtccgccaca ctcatatcat caagaacgtt gctgattgca tagcgccgcc
     2881 tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa gaatccggca gtaacaggcg
     2941 ttcgctttgc cacctggtca aaacaggcca gccagtgccg tagctcgcat ttatccagcg
     3001 gcatagcacc aatgtgctgt attacgtgat tattaaggcg ctttttcagg gcggcataat
     3061 ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt gagcgcatcg ccaaccgtta
     3121 ccggctttaa cgcttcctgt acggtgtaat taagctcatg acgtggattt tcccctctgc
     3181 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggttca gatcaggata
     3241 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
     3301 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
     3361 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
     3421 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
     3481 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
     3541 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
     3601 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
     3661 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
     3721 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
     3781 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
     3841 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
     3901 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
     3961 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
     4021 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
     4081 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
     4141 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
     4201 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
     4261 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
     4321 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
     4381 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
     4441 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
     4501 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
     4561 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
     4621 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
     4681 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
     4741 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
     4801 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
     4861 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
     4921 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
     4981 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
     5041 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
     5101 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
     5161 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
     5221 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
     5281 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
     5341 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
     5401 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
     5461 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
     5521 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
     5581 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
     5641 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
     5701 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
     5761 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
     5821 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
     5881 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
     5941 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
     6001 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagaaacac gtagttcaaa
     6061 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
     6121 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
     6181 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
     6241 aagcggtttg ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa
     6301 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
     6361 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
     6421 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
     6481 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
     6541 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
     6601 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
     6661 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
     6721 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
     6781 gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt taacaagcct cgtctgttgt
     6841 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
     6901 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
     6961 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
     7021 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
     7081 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
     7141 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
     7201 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
     7261 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
     7321 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
     7381 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
     7441 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
     7501 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
     7561 acccgcgacg acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg
     7621 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
     7681 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
     7741 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
     7801 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
     7861 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
     7921 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
     7981 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
     8041 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
     8101 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
     8161 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
     8221 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
     8281 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
     8341 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
     8401 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
     8461 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
     8521 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
     8581 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
     8641 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
     8701 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
     8761 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
     8821 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
     8881 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
     8941 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
     9001 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
     9061 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
     9121 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
     9181 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
     9241 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
     9301 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
     9361 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
     9421 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
     9481 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
     9541 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
     9601 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
     9661 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
     9721 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
     9781 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
     9841 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
     9901 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
     9961 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    10021 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    10081 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    10141 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    10201 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    10261 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    10321 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    10381 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    10441 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    10501 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    10561 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    10621 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    10681 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    10741 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    10801 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    10861 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    10921 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    10981 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    11041 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    11101 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    11161 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc
    11221 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    11281 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    11341 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    11401 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    11461 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    11521 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    11581 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    11641 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    11701 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    11761 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    11821 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    11881 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    11941 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    12001 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    12061 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    12121 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    12181 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    12241 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    12301 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    12361 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    12421 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    12481 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    12541 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    12601 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    12661 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    12721 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    12781 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    12841 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    12901 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    12961 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    13021 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    13081 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    13141 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    13201 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    13261 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    13321 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    13381 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    13441 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    13501 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    13561 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    13621 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    13681 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    13741 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    13801 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    13861 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    13921 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    13981 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    14041 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    14101 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    14161 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    14221 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    14281 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    14341 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    14401 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    14461 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    14521 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    14581 tcagacgcga tgcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    14641 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    14701 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    14761 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    14821 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    14881 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    14941 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    15001 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    15061 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    15121 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    15181 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    15241 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    15301 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc
    15361 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    15421 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    15481 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    15541 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    15601 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    15661 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    15721 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    15781 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    15841 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    15901 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    15961 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    16021 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    16081 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    16141 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    16201 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    16261 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    16321 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    16381 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    16441 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    16501 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    16561 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    16621 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    16681 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    16741 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    16801 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    16861 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    16921 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    16981 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    17041 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    17101 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    17161 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    17221 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    17281 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    17341 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    17401 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    17461 cattgttctt cagcgaacca ctgccgccgc tgttcagtta tttccctgcc aataccttgc
    17521 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaagctgac acgctggcgc
    17581 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    17641 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    17701 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    17761 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    17821 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    17881 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    17941 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    18001 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    18061 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    18121 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    18181 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    18241 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    18301 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    18361 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    18421 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    18481 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    18541 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    18601 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    18661 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    18721 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    18781 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    18841 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    18901 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    18961 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    19021 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    19081 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    19141 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    19201 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    19261 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    19321 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    19381 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    19441 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    19501 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    19561 aacaaagcgg ttcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    19621 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    19681 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    19741 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    19801 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    19861 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    19921 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    19981 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    20041 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    20101 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    20161 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    20221 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    20281 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    20341 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    20401 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    20461 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    20521 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    20581 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    20641 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    20701 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    20761 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    20821 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    20881 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    20941 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    21001 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    21061 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    21121 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    21181 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    21241 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    21301 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    21361 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    21421 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    21481 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    21541 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
    21601 agaaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
    21661 ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca
    21721 attattttta tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca
    21781 ttcacagtca ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa
    21841 agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg
    21901 ttctggcaac acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc
    21961 aaagcgccca cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc
    22021 gaccgctata tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa
    22081 agcgtggagt ctgcgctggc tgactatttt gcggccatcg ccaactgctt taccagcaaa
    22141 gacaccccgg caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga
    22201 gatatagcca atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt
    22261 ttatgtcaac gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca
    22321 gaattcctta attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg
    22381 gaaaaactga tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa
    22441 gtcgcaaaaa aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt
    22501 gttatacttt gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg
    22561 gatcgcggtc catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac
    22621 cgagcatttt ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag
    22681 tatcgctttc ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc
    22741 ccacaacagt ggcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac
    22801 cagcaacagc gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat
    22861 gggtcactgt gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg
    22921 ttgcagaaac aacctgagcg tattgcgggc cccgttcaaa cacgttcaga ctggccactg
    22981 ccgctacgcc caacgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca
    23041 cgggaacacc tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta
    23101 tcagacagaa gatgaaaagc gcgggaatga ctggaaatac ttaaggattc ggaggagtct
    23161 gaaggaatgc ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg
    23221 tccagggcgg ataaggcgtt tataccacat ccgccagtgt acgtcgatta atgcaacttc
    23281 aaacgcggac ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag
    23341 taaccatgca gcgcaatctg atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt
    23401 cgtccttcaa tcatcattga gccgcgcgtc aggttaccca tcaggctacc aacggtggag
    23461 aagttcgaca gcgataccag cgaaccatga tctttatact gataattttt cagcggctta
    23521 ccgttcatct gcgccagaat gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga
    23581 gcacgcggcg gaacaaagcc cccttccgga cgcgggcatg acgcgcagtc gccaatagcg
    23641 taaatgtctg gatcgcgagt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg
    23701 ttagtttcaa gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccat
    23761 accatcagat cagcctcaat atattcgcca tctttagtgt gcaggccgcc ttcatcagca
    23821 ctggtgacca tggtttgcgt cagcacgcga acgccaagtt tcgttagctc gttgtgggcc
    23881 gcagtagaga tacgtggcgg taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt
    23941 acgttcaggg cttcgttggt caggcctttg taaccgtagc tgtgcagttg cttgatcgcg
    24001 ttgtgcaatt cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc
    24061 actttgccat ttgcgcccag gttggcagag tatttcagga acagattcag catctcctgg
    24121 tggaagcgac gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacgcctggc
    24181 gtattgaaat cgttagaggt gctacccagc gccatgacca gggtgtcata ggcgatttta
    24241 cgttccggaa ccaacagttc acctttctcg tcgcgtagtt ctgcaatagt gattgttttc
    24301 gcttcacgat caatatcaat gacggaaccc aactggaact ggaaaccatg attgcgcgca
    24361 tgggccagat agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc
    24421 agcagtggtt tccacaggtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc
    24481 ttacgtccca gcttatgccc cagctgtgtt gccatttcca gcccaccagc accgccgccg
    24541 acaatcacaa tctttttcaa tggcgtagtc aacgtgaccc ccttaaattt attaaccaat
    24601 tgttaattaa tggttattaa catagctctt aattaacaac aggttacagc aatgaaaatg
    24661 ttcttcgcta ttgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc
    24721 aagttttgtt gcttaaaaga taggtaactt taccggggga gatcaaaaaa tagagtttat
    24781 tgtttattat ttgagcaaat tcggaagggt aatatagggt gaagcgtata tcgagggcgt
    24841 ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga ctgtcggatg
    24901 cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
    24961 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg
    25021 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
    25081 gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac aaaataacca
    25141 gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
    25201 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
    25261 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccaggc
    25321 caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg tccacttctt
    25381 taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
    25441 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
    25501 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctgctttc
    25561 tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt ccagcgagcc
    25621 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
    25681 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
    25741 aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata actgcccata
    25801 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa acccagttcc
    25861 tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg atcaacatca
    25921 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg
    25981 aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca cggtgtttct
    26041 ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
    26101 ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt ttgtggatcg
    26161 gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag cactggcgca
    26221 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
    26281 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
    26341 cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc
    26401 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca
    26461 tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
    26521 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
    26581 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
    26641 gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca gccagggaaa
    26701 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
    26761 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
    26821 gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg cgctatgcac
    26881 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg
    26941 cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct
    27001 ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa aacgtggttg
    27061 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
    27121 cgcgccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
    27181 aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc
    27241 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
    27301 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
    27361 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
    27421 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
    27481 cagaggcgct ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
    27541 tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
    27601 gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
    27661 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
    27721 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
    27781 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct
    27841 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
    27901 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
    27961 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
    28021 ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt gcgcccccag
    28081 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg
    28141 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
    28201 ggtaacgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt gaatatcaga
    28261 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
    28321 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
    28381 caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
    28441 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
    28501 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
    28561 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
    28621 acgtcgttca cagtcggaat gagaatattc ggaatgatca gaatatgcgt tggcgcttgc
    28681 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
    28741 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
    28801 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
    28861 taaacctgct ctttcaatct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
    28921 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
    28981 ggtttacacg acaaatatga atgcgtatat ttctcatttg catttgcatt tgcagcaaaa
    29041 ttattcgcac actaaatata accgctcatc accctcctcc ggcatgagcc tgacgacaac
    29101 ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagt
    29161 catcaccaaa ccgtcactac ttgccggttg catagcactg gcactattac cttctgccgc
    29221 ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca cagctccaga
    29281 tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac
    29341 tcaacgtgat attcctcagt cggtcactat tgttagccag cagcggatgg aagatcagca
    29401 gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga
    29461 ttccgatcgt gctctttatt attcccgcgg attccagatc gataactata tggttgatgg
    29521 tatccccacc tattttgaat cgcgctggaa tctgggcgac gcactttctg atatggcact
    29581 gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga cgggtaatcc
    29641 atctgcggca attaatatgg ttcgaaaaca cgcgacctgt cgtgaattta aaggcgatgt
    29701 ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac aaagcccact
    29761 caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata acgactcatg
    29821 gctggaccgc tacaatagtg aaaagacctt cttctcgggc attgtcgatg ctgatttagg
    29881 cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta atagcccaac
    29941 ctggggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg atcgcgcacg
    30001 cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct ttatgaccct
    30061 gaagcagcgg tttgctgata cctggcaagc gacactgaat gccacccact ctgaagtcga
    30121 atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt
    30181 tgggccatac agtaattatg gacctggctt tgatcatgtc ggcggcaccg gttggaacag
    30241 tggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtagttatg aattgtttgg
    30301 tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc gttcagttca
    30361 tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca
    30421 caagccgact ggtcatcaca gagcctggcg caggacgata ccacacatat gaaatcgtta
    30481 tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat
    30541 accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac
    30601 gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt
    30661 ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac
    30721 aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc
    30781 atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc
    30841 aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa
    30901 ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg ctatcttgca
    30961 gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc
    31021 accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt taactggcaa
    31081 aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc
    31141 tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg
    31201 aacgtcaata acctgttcga caaaacctac gataccaaca tggaaggttc tatcgtctac
    31261 ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac acctgtaaaa
    31321 aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg
    31381 tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca
    31441 gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta
    31501 gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt
    31561 tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgttgctttt
    31621 gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg
    31681 cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcggtaa cagccacagt
    31741 ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg
    31801 tcacgcatcc ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg
    31861 atgtacagga tcggcgcaac gaacatgaag aagaactcga tcggctcggt gataccggtc
    31921 aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcacg gttttctggt
    31981 ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag
    32041 ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg
    32101 aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag
    32161 atgtggtgca gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat gccaaacgca
    32221 actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc
    32281 ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc
    32341 acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg
    32401 ttaaacatgt acgctgcaat cgcaccggag ataatccctc cgagtacgcc agtatccgcc
    32461 aagtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc
    32521 atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta
    32581 ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa
    32641 ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa
    32701 ccgacgccca gcagaatacc tgcgataggt agtacggata ccggcagcat cagcgattta
    32761 ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat
    32821 gggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc
    32881 atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg
    32941 gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc
    33001 acaatatttc aaacgcggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt
    33061 agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg
    33121 tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca
    33181 acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct
    33241 tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc
    33301 aaggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt cacaatgccg
    33361 gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta
    33421 aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta
    33481 tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg
    33541 atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc
    33601 agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct
    33661 acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca
    33721 cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga
    33781 caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa
    33841 gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc
    33901 ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa
    33961 aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact gttcacgaat
    34021 gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc
    34081 ggcgaccagt cccggcacat caacattggt cacctgcgca gcgccatgat ggcgttttag
    34141 cgcatccatc agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt catgattaag
    34201 ggccgccagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa
    34261 tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc
    34321 ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag
    34381 ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg
    34441 taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg
    34501 tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc
    34561 ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac
    34621 ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta
    34681 atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg
    34741 gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc
    34801 catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc
    34861 taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc
    34921 acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca
    34981 tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc gcgggtgcga
    35041 ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt
    35101 tttaagccaa cttccggggt aacatcgaga tagagcgtta agtctgggcg aaaatccccg
    35161 agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccgccctga
    35221 tacgcctgag tggaaagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca
    35281 ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct
    35341 ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt
    35401 tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc
    35461 tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca
    35521 atgacgatat acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga
    35581 cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa
    35641 atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt
    35701 agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc
    35761 caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg
    35821 gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc
    35881 gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt
    35941 tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac
    36001 cattttcttg tgaactcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcaa
    36061 ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc
    36121 tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg
    36181 cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt
    36241 accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt
    36301 cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa aaacccgtgg
    36361 gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc cggtccctgg
    36421 cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac
    36481 cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa
    36541 taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac
    36601 gttgctgacg aaaaggtgac atcgccacag gcacgtacgg gcatcactgg cattaacgca
    36661 ttacaaataa ccatctcatc tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca
    36721 agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat gccgttaaca
    36781 cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa caaattagcc
    36841 gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc
    36901 tgctcaagat gagagcgaat caatacttgc tcaagccgat taagatgttt aatacctgca
    36961 agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc
    37021 aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc tggtccgctg
    37081 ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca
    37141 ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag
    37201 gaaatcatca accgctgaca agcatcctgt agtcgcagga tatgcgccga taacaaactg
    37261 actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga
    37321 tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc ggctccttat
    37381 cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga
    37441 aaaaaggccc gcaagcggac cttttatatg ggtggaaaat gacaacttag atctttttaa
    37501 agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc
    37561 cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca tccgggttat
    37621 ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt
    37681 ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca
    37741 cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag
    37801 ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat
    37861 cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca
    37921 tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc
    37981 gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga
    38041 agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag
    38101 ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc
    38161 cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt
    38221 tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca
    38281 tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga
    38341 tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc
    38401 caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc
    38461 cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag gcatccatct
    38521 tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa
    38581 atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg
    38641 caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc
    38701 cggtcacaac tacacgacgc ttagacacgt ttgtcctcca aggagggaaa aatgattcta
    38761 gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc
    38821 ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc
    38881 gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag
    38941 agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac
    39001 gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa
    39061 tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt
    39121 gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca
    39181 ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg
    39241 agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc
    39301 acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg
    39361 tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct
    39421 cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta cagtaatacc
    39481 gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc
    39541 agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat
    39601 acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga
    39661 tgaaaggtta gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt
    39721 atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat
    39781 tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc
    39841 gttggcacct aaataatcac tgatcgcctg agcgccattt tcactggtcg cagtgccaat
    39901 aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc
    39961 accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag
    40021 cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg
    40081 tttcgtcagg ccagtaagca ccttgcccgg gccgacttca tagagatgtt ctacgccttg
    40141 cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag
    40201 tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacaga
    40261 aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg
    40321 tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagcgcgc gttttgcgcc
    40381 agcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac
    40441 ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag cttcttcaca
    40501 cgctttcgca ataggcgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc
    40561 cggtacggct tcttgcatga acttaccgcg catctcaacc agacgcaccg catcagcgaa
    40621 atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat
    40681 tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag atgcagtcaa
    40741 cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct gctgggtcag
    40801 cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg
    40861 atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac
    40921 aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cagagcccca
    40981 agtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc
    41041 gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc
    41101 cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg cactgataat
    41161 acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc
    41221 cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt
    41281 gcccgccatc gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt
    41341 cagcaattca ccataactac cgtcggcatg cagatgggtg gaaatgattc ccggctcttc
    41401 agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc
    41461 tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc
    41521 cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg cacaggctgc
    41581 tgcaacgtca aatgccgggc aacctttaat gtccaacatg ctttgaatct gacaagctgc
    41641 gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt
    41701 ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt
    41761 ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc
    41821 agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct
    41881 gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc
    41941 acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac
    42001 cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag
    42061 gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat
    42121 tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat
    42181 actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta
    42241 ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga
    42301 acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa
    42361 ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga
    42421 tagaagggat tgttttaagc accgctgagg catcccgaat actgtcaaga ccctttactt
    42481 cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag
    42541 ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg
    42601 cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg
    42661 ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc
    42721 cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca
    42781 tacgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca tcactggcga
    42841 taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa
    42901 gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat
    42961 tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat
    43021 ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt
    43081 tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac
    43141 cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga
    43201 agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc
    43261 acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg
    43321 gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc
    43381 aaatgggttt ggcttttgca cttcttcagg cagttcacca aagaccatgt ccgcttcgga
    43441 cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc
    43501 ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc
    43561 ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta
    43621 gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc
    43681 atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac
    43741 atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat
    43801 accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca ggggtaattt
    43861 taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc
    43921 agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg
    43981 ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc
    44041 atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacctcg
    44101 ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcacctgag
    44161 gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa
    44221 gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag
    44281 gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg
    44341 caattacgca aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat
    44401 tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt ccgtcaccta
    44461 agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc
    44521 tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagagggacg tgaccctaac
    44581 acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac
    44641 ccgctgatgg gatagttgtt tgcgccggat gcggttacgt cgcatccggc aatcggtgca
    44701 gacggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatatg
    44761 cttgtattag cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt catccatcgg
    44821 cgcttcgata cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg
    44881 caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc
    44941 accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg
    45001 atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc
    45061 cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc
    45121 gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg
    45181 accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag
    45241 agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag
    45301 acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac
    45361 gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag
    45421 gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg
    45481 cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc
    45541 cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt cacctttacg
    45601 caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc
    45661 gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt
    45721 tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt
    45781 atatttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac
    45841 caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat
    45901 cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa
    45961 aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg
    46021 taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga
    46081 tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc
    46141 gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt
    46201 ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc
    46261 agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg
    46321 caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg
    46381 atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc
    46441 gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc
    46501 tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc
    46561 ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag
    46621 agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg
    46681 ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc
    46741 gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg
    46801 tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca atgggattta agcttccgcc
    46861 tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg ttcctgattc
    46921 atcaggagag caacgtaatc gttcgcgcct tccgcgatta tttacgtcag gatatcggcg
    46981 aaatccttat cgataacccg aaagtcctcg aactggcacg tcagcatatc gctgcattag
    47041 gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc
    47101 actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccatccg
    47161 gtggttccat tgttatcgac agcaccgaag cgttaacagc catcgacatc aactccgcac
    47221 gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg
    47281 atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca
    47341 tcgacatgac gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc
    47401 gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt
    47461 cccgccagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc ccgcgctgct
    47521 ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtttgatcg
    47581 aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg
    47641 cttcttatct gctgaatgaa aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg
    47701 gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac cccgcactac cacgtgttgc
    47761 gcgtgcgcaa aggggaagaa acgccgacct taagctacat gctgccgaag ctgcatgaag
    47821 aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtcct gaacaacctg
    47881 cactggcaac ctttgccatg ccggatgtac cgccagcgcc aaccccagct gaacctgccg
    47941 cgactgtcgt agccccagca ccgaaagctg caacggcaac accagcagct cctgcacaac
    48001 ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag
    48061 ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa accggaacgt caacaggatc
    48121 gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga gcgccgcgac acccgtagtg
    48181 aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc
    48241 agcaaactgc cgagacgcgt gagagccgtc agcaggctga ggtaacggaa aaagcacgta
    48301 ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac
    48361 gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg
    48421 aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga
    48481 aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa
    48541 ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa ctggtgaaag
    48601 tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata
    48661 accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga
    48721 gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt
    48781 tgaccgtagc gtgcacgtct ccggaattgg cctctggcaa agtctggatc cgctatccaa
    48841 ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagaa gtacaagtgc
    48901 agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag
    48961 ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg
    49021 aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg
    49081 tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca gaaccggtgg
    49141 ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    49201 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    49261 aagttgaaac ggtggccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    49321 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    49381 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    49441 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    49501 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    49561 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg
    49621 gcgatcccag agcgggcaga caaaacatac ccagtttcat gatatgtttt aaagcgagct
    49681 tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc
    49741 tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc
    49801 agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaagga atccagcgac
    49861 tccagtttgt tcagctgcgt gcctaattcc gcgcgaacgg taaggacatt attcagcgag
    49921 tttttcagtc cacggttggt tttatccaat gccgcagcgg cgatttcttt atccgcttcg
    49981 ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc aaaaagattg
    50041 gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact gtcgaaaatt
    50101 ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg cttaatactt
    50161 tctgttccgc caacataatc accgttagcc tcactaaacg gcgcagtctc tgttttataa
    50221 ccggcaaaaa tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca
    50281 cgaagcccct gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg
    50341 ctggcgtaga caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca
    50401 ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt
    50461 tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg
    50521 ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa
    50581 ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct
    50641 ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg
    50701 cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga
    50761 caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc
    50821 tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt
    50881 cgttaaagga tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct
    50941 gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg attttcgctt
    51001 cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc
    51061 tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac
    51121 cgttggcatc tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg acctgccact
    51181 gattattatc gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg
    51241 tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga
    51301 tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttggctgt
    51361 tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat
    51421 cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga
    51481 tctcaatatt gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa gggtcggcgc
    51541 tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg
    51601 catcgtgatg ttataagtac cgccatcctg aacgctgact tctacgccaa caatctggtt
    51661 taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg cccctgcccc
    51721 cacgcctgtc aggcgcgaga tttgatcgtt caggctggca atttgtttag cgtaattgtt
    51781 gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt cgcgcagata
    51841 ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca gcgcctggcg
    51901 cgccgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga aatcctgcat
    51961 ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg acatctgctc
    52021 atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt tggtaataaa
    52081 cgtatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc agccgccagc
    52141 gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc cggcaacgtt
    52201 atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct
    52261 catggcgtta ttgatcaagc tggacatgga ggttccttat aagccttcaa ttacagagta
    52321 ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat gttcatgctg
    52381 taggttttgc tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt ggtgagttta
    52441 cgggcatagt gaggatcggt agcatagccc gcgtcctgta ggacctgcgc cccctgttcc
    52501 gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa
    52561 tccgacaatg cttccagata cgagctgtag acccgaaact tcgcttttac tttcttcgct
    52621 tcgccgtttt catattcagt cgtggtgatt tcagtgactt gccctttcca gttgccagag
    52681 gctttgacac caaacaggtt atagcttggc tcgccgtttt cgcggcggat ttgccgttgc
    52741 ccccaaccag attcaagcgc agcctgagcg aggatcaaat gatgtggcac accgctttgc
    52801 tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctctact gtcacccggc
    52861 agcgaatcat cgtagttacg tggtacggcc ttttgcacca gctgcgaaag cgtctggttt
    52921 tgataacgca ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt ggactcctct
    52981 ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc cagacctttg
    53041 cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag acgagtgtgc
    53101 tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc
    53161 acgaacatcc cttccacctg acgggccacc ggacggatat ttgccgccgg atcttcgctc
    53221 gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc cagtagtttg
    53281 ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca ctttgcattg
    53341 attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc gcacgcacca
    53401 cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc tggcgtaaat
    53461 cgatctgcgt ttgtggagta acaacagtct gtccaccacc aaacggtgta tctggctggc
    53521 tgacattggc ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt
    53581 cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag
    53641 cgtcctgcgg ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg
    53701 aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc
    53761 catatccacg cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt
    53821 cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa
    53881 taaccgcacc attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc
    53941 ctgcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac
    54001 tgtcaacgcc cttaagcggt gtcatcaaca acgtacctcc acgcaagctt ttggcattac
    54061 ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgacg
    54121 ctgtcaccat taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa
    54181 ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct
    54241 ggtcaccagt gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta
    54301 ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta
    54361 gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt
    54421 aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac
    54481 gtattcaata cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt
    54541 gcgtggatta accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg
    54601 tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa
    54661 cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc
    54721 ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt
    54781 atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc
    54841 gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc
    54901 ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt
    54961 ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt
    55021 ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt
    55081 atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc
    55141 caccggttat gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg
    55201 ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca
    55261 cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg
    55321 gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc
    55381 tctccagtcc ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag
    55441 ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg
    55501 cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc
    55561 cgttctgatc cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca
    55621 ttacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca
    55681 gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta
    55741 atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat
    55801 aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca
    55861 ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc
    55921 ataatgaact gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg
    55981 cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg
    56041 cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc
    56101 acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc
    56161 gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc
    56221 cgacatcaca cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc
    56281 tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac
    56341 cagtttcaga cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga
    56401 gattgtgcca tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc
    56461 ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct
    56521 gccattacgc gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc
    56581 aacgcaacgt ccagcgggcg cgaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc
    56641 ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg
    56701 cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg
    56761 gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga
    56821 tccattgagc tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc
    56881 ctgggttttg atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac
    56941 cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca gggtgccaaa
    57001 gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac
    57061 gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc
    57121 cagcagttgg gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg
    57181 ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc
    57241 gccggtattt tgctgcatgg agttgaggaa gctcagacta aacgtggcgg gttctgcacc
    57301 gttaattgcg ccggttgcga tattattcgc catcgcacca tccactaatg tgccattagc
    57361 attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt ggatcactgc tatcctgggt
    57421 gtagacctgc cagttattat ccccggtctt cacaaaatag acgctcatgt catgagcatt
    57481 accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc
    57541 gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc
    57601 cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc
    57661 cccttgctga atagtcggcg gcgtaccggt taccgggtaa cccgtcagct gtaaaccttg
    57721 cgtattcaac aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac
    57781 cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc
    57841 tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac
    57901 ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa
    57961 gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc
    58021 gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta
    58081 aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt
    58141 accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag
    58201 ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct
    58261 gccatcagtc aacgaaccgt cccaggtgaa actgtgaact ccggcggtca gttcaccaat
    58321 atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc
    58381 cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc
    58441 ggttcccgca agaacagttg tgccggggat catcacgccg tgaccgatca ggttactggc
    58501 ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt
    58561 ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat
    58621 tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa aactgctttg
    58681 taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt
    58741 tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta
    58801 agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg
    58861 cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg catttttacg
    58921 tagccctttg catctgccag cggattacct ggttcataaa ccagtttgtc cggggcctga
    58981 ctttcttaac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct
    59041 ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg ctatcagcat
    59101 ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac gccgacccgg
    59161 cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca
    59221 tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg
    59281 caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg
    59341 gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg atgtgttgcg
    59401 ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt
    59461 taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga
    59521 tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    59581 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    59641 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    59701 atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga
    59761 aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagta
    59821 cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc gcgggggtaa
    59881 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca tgcgagcagc
    59941 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    60001 gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg
    60061 ccgcgatgcc tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgtg
    60121 gacgcctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    60181 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    60241 ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg tttagcgcca
    60301 acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg gtgcgcatgg
    60361 tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt
    60421 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    60481 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct gtaagcaccg
    60541 ttcaaccgcg cgaaaccacc gacgcgccgg taacgaacag ccgggcggca aaaacaaccg
    60601 cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    60661 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    60721 taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc
    60781 agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt
    60841 aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg gcagatcaac
    60901 ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac
    60961 ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat tagtcaacgc
    61021 tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    61081 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    61141 gaaccgacgc tatatggggc gaacggtcag acctcaacac cccatcgcgg cagtaaaaag
    61201 atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt
    61261 ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc
    61321 aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga
    61381 ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac
    61441 caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg
    61501 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    61561 cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt
    61621 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    61681 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    61741 catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc
    61801 tgaaagaatg cccaacgcaa ccgcactcgg cagtgccaac aggaaacaaa gacgcaaccc
    61861 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    61921 cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg
    61981 gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    62041 gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac
    62101 cacgcgcatt gctccagcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt
    62161 cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc
    62221 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    62281 gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat
    62341 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    62401 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    62461 cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag
    62521 gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc
    62581 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    62641 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    62701 ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    62761 ggcggccagc gattttaata aattcatcgg tgttctaatc cggacccacg ggcaaaacgc
    62821 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctag attatggtga attgctaccg
    62881 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    62941 gcgccagcac tgcctattcg ccggcggttt gcggaactgt ctgattttgc acgcattcaa
    63001 tgaagtgccg cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa
    63061 tcggtttatt cactacaccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt
    63121 cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat
    63181 gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt
    63241 cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc
    63301 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    63361 gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg gcgagttgcg
    63421 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    63481 ttttccgcgc cgccagttcc accagccttt cagcatcgcg cagattttct gccagcggtt
    63541 tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca aagtggctgg
    63601 cggtgctgga gtgcacaaaa accgcatcac aactggcggc aaggctggat aacgaatcgg
    63661 cataaggaat gcgccagctg tcacaaattg gcagggcttt ggcgcgcgta ggcgaccaag
    63721 ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa
    63781 tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc cagatgggcc
    63841 agcaacgaat caagacgttg tttcagttct gccacttcga tttccagggc ttcgacgcgg
    63901 gtctataaat caccgtcaac catgttcgac atagccgtta ccgccggctg atcttcaacc
    63961 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    64021 accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc cacttccgcc
    64081 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    64141 ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt cagatcgcca
    64201 aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa accgctcact
    64261 gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc ggattcactc
    64321 agattcatga ccggtttacg gttcgttttc tgattacagg ccgtgactac accattgact
    64381 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aattacgcga
    64441 gcttccaagg cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    64501 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    64561 tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt
    64621 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    64681 ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    64741 accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg caacgccaat
    64801 aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa
    64861 ttcgttaatc atccctagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg
    64921 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg ccagacgcca
    64981 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    65041 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    65101 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc
    65161 tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata
    65221 ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    65281 ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt
    65341 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    65401 tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct
    65461 acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg
    65521 tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg
    65581 tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac
    65641 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgtgg
    65701 tcgttttttc tcgctgttga tgatgcagga cagtgccagt gcggtcattg gcgcattgtt
    65761 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt
    65821 tgtgctatgt gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg
    65881 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    65941 cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    66001 ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    66061 tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    66121 acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt
    66181 cagcggccta caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc
    66241 cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc gcggcagcta
    66301 tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg
    66361 ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg
    66421 aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgccgc
    66481 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    66541 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    66601 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    66661 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    66721 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga
    66781 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    66841 gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt
    66901 tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc
    66961 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    67021 tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    67081 agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg
    67141 gccaagccgc gttgctaatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    67201 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    67261 gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg
    67321 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    67381 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    67441 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    67501 gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt cgcgaagagc
    67561 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc
    67621 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    67681 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    67741 cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc
    67801 aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg
    67861 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag
    67921 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    67981 ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca tcccaggtat
    68041 taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa
    68101 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    68161 cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac
    68221 ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta
    68281 atgagctgga gcgcggattt aatgaagccg tgttcaccgc tgcaaaactt tacccggcaa
    68341 acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg
    68401 agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata
    68461 tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc
    68521 gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg
    68581 tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc
    68641 gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac
    68701 gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc
    68761 tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg
    68821 gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    68881 ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat ggcttgccgg
    68941 tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac
    69001 tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    69061 aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa taaccagtat
    69121 gttcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca
    69181 gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct
    69241 gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc
    69301 gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc
    69361 gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg atcgccccgg
    69421 cttttttatt ttacttacga attttgagta caaaatttct acacttcctc aaaataattg
    69481 cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa
    69541 gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
    69601 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
    69661 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
    69721 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
    69781 aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa ccactgatgt
    69841 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
    69901 cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
    69961 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
    70021 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
    70081 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
    70141 ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc
    70201 ttatggtgaa ggcgaaaagt atgtcccgtt ggtcctccgc gcgcaaacgc tgtgggatga
    70261 actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg
    70321 cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa
    70381 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga
    70441 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
    70501 aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac
    70561 cgcaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc
    70621 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
    70681 ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa
    70741 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
    70801 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
    70861 tgaacgtgtt ccgtttgcgg aagtggccag tgatgggtca gaagccttcc cgttcttgcg
    70921 caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
    70981 gcctgacgaa gattttatta tcgataccct acccggccac gataatacac tgctcattac
    71041 cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
    71101 tgcgcaagac caaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
    71161 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
    71221 ttgcactatc tcatcaataa tattcgcgag catttgatgc tttatctttt tctctgggga
    71281 ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc actgacataa
    71341 actgacaaat atttaatctc cctttcattt agcgtgaaaa attgaatgat attattcctg
    71401 cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat gaagattctt
    71461 tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg ttatttttgt
    71521 gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta ttggatgcac
    71581 ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc ttccattggc
    71641 taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc acgctcagtt
    71701 attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt attttgttaa
    71761 tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc
    71821 cgagttattc gcccatgact cgtcttgccg caaaagctgg acatctcgca ttgtacctat
    71881 tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt aaaccgatca
    71941 gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca caggcagact
    72001 ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtct gttatgcacg
    72061 gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag cgcatgctgg
    72121 gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct
    72181 tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa attgacaaag
    72241 aaggtcagca cgcctttgtt aacttccgca tacagcatct tggctatagc tggttatatg
    72301 gcacctttaa agatttcgac ggtactttta cctttgatga aaaaaatccg gctgccgata
    72361 aagtgaatgt gacaattaac accaccagcg tcgatactaa ccacgccgaa cgcgataaac
    72421 atcttcgcag tgcggatttt ctcaatacca caaaatatcc acaggcaaca ttcacctcca
    72481 ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg ctgaatggcg
    72541 taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac ccatggggcg
    72601 gtaaacgtgc aggctttgag gccgaaggca aaattaagct caaagacttc aatatcaaga
    72661 cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa ggcgtacagc
    72721 agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt tattctgttg
    72781 gatcaggaat acccagtgtt gtattcagac gtccacgaga cttattaaag atcttattgc
    72841 cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc
    72901 agcaaatctc actacaacaa cctttgtatt tttccgcgca tactggacac tgaataaaca
    72961 gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg ccgcactggt
    73021 ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca aaaacaaaat
    73081 ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg tattcaataa
    73141 ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag gcactggcct
    73201 tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta tgtgcctgca
    73261 tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt tcgagcgcgt
    73321 tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat agtgcatcgg
    73381 gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca ttgctggcat
    73441 caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc atgcgcagta
    73501 cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc tctaaagccg
    73561 ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt
    73621 gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc gcggtaaaca
    73681 gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac ttgtaaaacg
    73741 aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttaacgcg tcgttggaaa
    73801 tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag taaaaatccg
    73861 cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta catttgcgca
    73921 ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag ccacaaattt
    73981 tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgtataggtt gaaaaacagg
    74041 attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg ttattggtta
    74101 acctggttgg gtattggcgt actttggttg gtcgtgcaat tgccctaccc gattatctac
    74161 cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt
    74221 gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg
    74281 gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggtat ggcgtggttc
    74341 tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca cattcgtgac
    74401 gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac actggagctg
    74461 ggcgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc gaacgataat
    74521 ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc gatgctcgac
    74581 cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca
    74641 ccagatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc cgttgagcag
    74701 gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg tctggtgccc
    74761 ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct gccaccagag
    74821 tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa
    74881 aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt taaaacacgc
    74941 ccggaaggcg ttccttcacg ctattaaatc tcccatgccg gatgcttcag aatggcatcc
    75001 ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat tgcgccccct
    75061 ggaagtcggg cgcataatta gtgtgtttat cttttcttct tatgttcacc gcgcctggcg
    75121 caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa ctggaaacga
    75181 aacctgatcg tcgcctggct aggctgtttt cttaccgggg ccgccttcag tctggtaatg
    75241 cccttcttac ccctctacgt tgagcagctt ggtgttaccg gccactccgc cctgaatatg
    75301 tggtccggta ttgtcttcag tataatgggt tcctaaacac atcattttag gtattgaata
    75361 tgagacgggc aatcagctac atcagatttt caagtgaacg acaactgaaa ggggactctg
    75421 tacgccgaca atctaagctg gttactgact ggcttgataa gaaccctgag ttttacttgg
    75481 attcatcctt gagctttaaa gatttgggga aaagtgcttt tagtggtaag cacctgaaag
    75541 gtggtctagg tgacttttta accgctatcg agaaagggct tgttaaagct ggtgatacgc
    75601 tactaatcga gagtttagac cgtctgtcac gtcaggatat tgatattgct tctgagctat
    75661 tacgccgtat cttaagggct ggtgtagatg tagtcacact gagcgatgga gaacattaca
    75721 cacgagaaag tttaaaagac ccgttagccc tgattaaaag cattctgata atgcagcggg
    75781 cgcatgagga aagtttgaga aagtctgagc gtgtccaagc tgcatggaac cgtaaaaaag
    75841 aactcatctc cgaaggta
//
LOCUS       DBKELA010000003        75616 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.003, whole genome shotgun sequence.
ACCESSION   DBKELA010000003 DBKELA010000000
VERSION     DBKELA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.003"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(435..641)
                     /locus_tag="ACUQR0_000160"
     CDS             complement(435..641)
                     /locus_tag="ACUQR0_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HHV9107368.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACUQR0_000161"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACUQR0_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HHV9107369.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACUQR0_000162"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACUQR0_000162"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HHV9107370.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACUQR0_000163"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACUQR0_000163"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACUQR0_000164"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACUQR0_000164"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HHV9107371.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACUQR0_000165"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACUQR0_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="ACUQR0_000166"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="ACUQR0_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HHV9107372.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8876..9010
                     /locus_tag="ACUQR0_000167"
     CDS             8876..9010
                     /locus_tag="ACUQR0_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HHV9107373.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            8989..9093
                     /gene="speFL"
                     /locus_tag="ACUQR0_000168"
     CDS             8989..9093
                     /gene="speFL"
                     /locus_tag="ACUQR0_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HHV9107374.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11601
                     /gene="speF"
                     /locus_tag="ACUQR0_000169"
                     /pseudo
     CDS             9404..11601
                     /gene="speF"
                     /locus_tag="ACUQR0_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11598..12917
                     /gene="potE"
                     /locus_tag="ACUQR0_000170"
     CDS             11598..12917
                     /gene="potE"
                     /locus_tag="ACUQR0_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HHV9107375.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="ACUQR0_000171"
     CDS             complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="ACUQR0_000171"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HHV9107376.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="ACUQR0_000172"
     CDS             complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="ACUQR0_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HHV9107377.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15369..16133
                     /gene="ybfF"
                     /locus_tag="ACUQR0_000173"
     CDS             15369..16133
                     /gene="ybfF"
                     /locus_tag="ACUQR0_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HHV9107378.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16273..16566
                     /gene="ybfE"
                     /locus_tag="ACUQR0_000174"
     CDS             16273..16566
                     /gene="ybfE"
                     /locus_tag="ACUQR0_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HHV9107379.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16706..17236
                     /gene="fldA"
                     /locus_tag="ACUQR0_000175"
     CDS             16706..17236
                     /gene="fldA"
                     /locus_tag="ACUQR0_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HHV9107380.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17446..17532
                     /gene="uof"
                     /locus_tag="ACUQR0_000176"
     CDS             17446..17532
                     /gene="uof"
                     /locus_tag="ACUQR0_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HHV9107381.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17525..17971
                     /gene="fur"
                     /locus_tag="ACUQR0_000177"
     CDS             17525..17971
                     /gene="fur"
                     /locus_tag="ACUQR0_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HHV9107382.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18055..18381)
                     /gene="chiQ"
                     /locus_tag="ACUQR0_000178"
     CDS             complement(18055..18381)
                     /gene="chiQ"
                     /locus_tag="ACUQR0_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HHV9107383.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="ACUQR0_000179"
     CDS             complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="ACUQR0_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0052778 - diacetylchitobiose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin ChiP"
                     /protein_id="HHV9107384.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="ACUQR0_000180"
     CDS             complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="ACUQR0_000180"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HHV9107385.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="ACUQR0_000181"
     CDS             complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="ACUQR0_000181"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HHV9107386.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24549..25349
                     /gene="nagB"
                     /locus_tag="ACUQR0_000182"
     CDS             24549..25349
                     /gene="nagB"
                     /locus_tag="ACUQR0_000182"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HHV9107387.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25409..26557
                     /gene="nagA"
                     /locus_tag="ACUQR0_000183"
     CDS             25409..26557
                     /gene="nagA"
                     /locus_tag="ACUQR0_000183"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HHV9107388.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26566..27786
                     /gene="nagC"
                     /locus_tag="ACUQR0_000184"
                     /gene_synonym="nagR"
     CDS             26566..27786
                     /gene="nagC"
                     /locus_tag="ACUQR0_000184"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HHV9107389.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27834..28586
                     /gene="nagD"
                     /locus_tag="ACUQR0_000185"
     CDS             27834..28586
                     /gene="nagD"
                     /locus_tag="ACUQR0_000185"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HHV9107390.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28983..30647
                     /gene="asnB"
                     /locus_tag="ACUQR0_000186"
     CDS             28983..30647
                     /gene="asnB"
                     /locus_tag="ACUQR0_000186"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HHV9107391.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31026..31102
                     /locus_tag="ACUQR0_000187"
     tRNA            31026..31102
                     /locus_tag="ACUQR0_000187"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31060..31062,aa:Met,seq:cat)
     gene            31111..31195
                     /locus_tag="ACUQR0_000188"
     tRNA            31111..31195
                     /locus_tag="ACUQR0_000188"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31145..31147,aa:Leu,seq:tag)
     gene            31219..31293
                     /locus_tag="ACUQR0_000189"
     tRNA            31219..31293
                     /locus_tag="ACUQR0_000189"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31251..31253,aa:Gln,seq:ttg)
     gene            31328..31402
                     /locus_tag="ACUQR0_000190"
     tRNA            31328..31402
                     /locus_tag="ACUQR0_000190"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31360..31362,aa:Gln,seq:ttg)
     gene            31418..31494
                     /locus_tag="ACUQR0_000191"
     tRNA            31418..31494
                     /locus_tag="ACUQR0_000191"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31452..31454,aa:Met,seq:cat)
     gene            31543..31617
                     /locus_tag="ACUQR0_000192"
     tRNA            31543..31617
                     /locus_tag="ACUQR0_000192"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31575..31577,aa:Gln,seq:ctg)
     gene            31655..31729
                     /locus_tag="ACUQR0_000193"
     tRNA            31655..31729
                     /locus_tag="ACUQR0_000193"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31687..31689,aa:Gln,seq:ctg)
     gene            complement(31883..33058)
                     /gene="ubiF"
                     /locus_tag="ACUQR0_000194"
     CDS             complement(31883..33058)
                     /gene="ubiF"
                     /locus_tag="ACUQR0_000194"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HHV9107392.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33204..34628
                     /gene="miaB"
                     /locus_tag="ACUQR0_000195"
     CDS             33204..34628
                     /gene="miaB"
                     /locus_tag="ACUQR0_000195"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HHV9107393.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34781..35821
                     /locus_tag="ACUQR0_000196"
     CDS             34781..35821
                     /locus_tag="ACUQR0_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HHV9107394.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35818..36285
                     /gene="ybeY"
                     /locus_tag="ACUQR0_000197"
     CDS             35818..36285
                     /gene="ybeY"
                     /locus_tag="ACUQR0_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HHV9107395.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36375..37253
                     /gene="corC"
                     /locus_tag="ACUQR0_000198"
     CDS             36375..37253
                     /gene="corC"
                     /locus_tag="ACUQR0_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HHV9107396.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37278..38816
                     /gene="lnt"
                     /locus_tag="ACUQR0_000199"
     CDS             37278..38816
                     /gene="lnt"
                     /locus_tag="ACUQR0_000199"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HHV9107397.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39213..40121
                     /gene="gltI"
                     /locus_tag="ACUQR0_000200"
     CDS             39213..40121
                     /gene="gltI"
                     /locus_tag="ACUQR0_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HHV9107398.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40291..41031
                     /gene="gltJ"
                     /locus_tag="ACUQR0_000201"
     CDS             40291..41031
                     /gene="gltJ"
                     /locus_tag="ACUQR0_000201"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HHV9107399.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41031..41705
                     /gene="gltK"
                     /locus_tag="ACUQR0_000202"
     CDS             41031..41705
                     /gene="gltK"
                     /locus_tag="ACUQR0_000202"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HHV9107400.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41705..42430
                     /gene="gltL"
                     /locus_tag="ACUQR0_000203"
     CDS             41705..42430
                     /gene="gltL"
                     /locus_tag="ACUQR0_000203"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HHV9107401.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42548..43483
                     /gene="rihA"
                     /locus_tag="ACUQR0_000204"
     CDS             42548..43483
                     /gene="rihA"
                     /locus_tag="ACUQR0_000204"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HHV9107402.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43567..45228
                     /gene="hscC"
                     /locus_tag="ACUQR0_000205"
                     /pseudo
     CDS             43567..45228
                     /gene="hscC"
                     /locus_tag="ACUQR0_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45288..46739)
                     /gene="djlC"
                     /locus_tag="ACUQR0_000206"
     CDS             complement(45288..46739)
                     /gene="djlC"
                     /locus_tag="ACUQR0_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HHV9107403.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46736..47443)
                     /locus_tag="ACUQR0_000207"
     CDS             complement(46736..47443)
                     /locus_tag="ACUQR0_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HHV9107404.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47545..48099
                     /locus_tag="ACUQR0_000208"
     CDS             47545..48099
                     /locus_tag="ACUQR0_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHV9107405.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48109..49536)
                     /locus_tag="ACUQR0_000209"
     CDS             complement(48109..49536)
                     /locus_tag="ACUQR0_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HHV9107406.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49533..50240)
                     /locus_tag="ACUQR0_000210"
     CDS             complement(49533..50240)
                     /locus_tag="ACUQR0_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HHV9107407.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50404..51381
                     /gene="ybeQ"
                     /locus_tag="ACUQR0_000211"
     CDS             50404..51381
                     /gene="ybeQ"
                     /locus_tag="ACUQR0_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HHV9107408.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51451..51933)
                     /locus_tag="ACUQR0_000212"
     CDS             complement(51451..51933)
                     /locus_tag="ACUQR0_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HHV9107409.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52168..54750
                     /gene="leuS"
                     /locus_tag="ACUQR0_000213"
     CDS             52168..54750
                     /gene="leuS"
                     /locus_tag="ACUQR0_000213"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HHV9107410.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54765..55346
                     /gene="lptE"
                     /locus_tag="ACUQR0_000214"
     CDS             54765..55346
                     /gene="lptE"
                     /locus_tag="ACUQR0_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HHV9107411.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55346..56377
                     /gene="holA"
                     /locus_tag="ACUQR0_000215"
     CDS             55346..56377
                     /gene="holA"
                     /locus_tag="ACUQR0_000215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HHV9107412.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56379..57020
                     /gene="nadD"
                     /locus_tag="ACUQR0_000216"
     CDS             56379..57020
                     /gene="nadD"
                     /locus_tag="ACUQR0_000216"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HHV9107413.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57044..57655
                     /locus_tag="ACUQR0_000217"
     CDS             57044..57655
                     /locus_tag="ACUQR0_000217"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HHV9107414.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57915..58232
                     /gene="rsfS"
                     /locus_tag="ACUQR0_000218"
     CDS             57915..58232
                     /gene="rsfS"
                     /locus_tag="ACUQR0_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HHV9107415.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58236..58703
                     /gene="rlmH"
                     /locus_tag="ACUQR0_000219"
     CDS             58236..58703
                     /gene="rlmH"
                     /locus_tag="ACUQR0_000219"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HHV9107416.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58734..60635
                     /gene="mrdA"
                     /locus_tag="ACUQR0_000220"
     CDS             58734..60635
                     /gene="mrdA"
                     /locus_tag="ACUQR0_000220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HHV9107417.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60638..61750
                     /gene="mrdB"
                     /locus_tag="ACUQR0_000221"
                     /gene_synonym="rodA"
     CDS             60638..61750
                     /gene="mrdB"
                     /locus_tag="ACUQR0_000221"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HHV9107418.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGMWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61761..62849
                     /gene="rlpA"
                     /locus_tag="ACUQR0_000222"
     CDS             61761..62849
                     /gene="rlpA"
                     /locus_tag="ACUQR0_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HHV9107419.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62988..64199
                     /gene="dacA"
                     /locus_tag="ACUQR0_000223"
     CDS             62988..64199
                     /gene="dacA"
                     /locus_tag="ACUQR0_000223"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HHV9107420.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64310..64573
                     /gene="ybeD"
                     /locus_tag="ACUQR0_000224"
     CDS             64310..64573
                     /gene="ybeD"
                     /locus_tag="ACUQR0_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HHV9107421.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64674..65315
                     /gene="lipB"
                     /locus_tag="ACUQR0_000225"
     CDS             64674..65315
                     /gene="lipB"
                     /locus_tag="ACUQR0_000225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HHV9107422.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65574..66527
                     /locus_tag="ACUQR0_000226"
     CDS             65574..66527
                     /locus_tag="ACUQR0_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HHV9107423.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66736..67701
                     /gene="lipA"
                     /locus_tag="ACUQR0_000227"
     CDS             66736..67701
                     /gene="lipA"
                     /locus_tag="ACUQR0_000227"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HHV9107424.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67802..68005)
                     /gene="tatE"
                     /locus_tag="ACUQR0_000228"
     CDS             complement(67802..68005)
                     /gene="tatE"
                     /locus_tag="ACUQR0_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HHV9107425.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68134..68922)
                     /locus_tag="ACUQR0_000229"
     CDS             complement(68134..68922)
                     /locus_tag="ACUQR0_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HHV9107426.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69015..69398
                     /gene="crcB"
                     /locus_tag="ACUQR0_000230"
     CDS             69015..69398
                     /gene="crcB"
                     /locus_tag="ACUQR0_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HHV9107427.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69452..69661)
                     /gene="cspE"
                     /locus_tag="ACUQR0_000231"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69452..69661)
                     /gene="cspE"
                     /locus_tag="ACUQR0_000231"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HHV9107428.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69836..70396)
                     /gene="pagP"
                     /locus_tag="ACUQR0_000232"
     CDS             complement(69836..70396)
                     /gene="pagP"
                     /locus_tag="ACUQR0_000232"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HHV9107429.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70984..72369
                     /gene="dcuC"
                     /locus_tag="ACUQR0_000233"
     CDS             70984..72369
                     /gene="dcuC"
                     /locus_tag="ACUQR0_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HHV9107430.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72410..72751)
                     /locus_tag="ACUQR0_000234"
                     /pseudo
     CDS             complement(72410..72751)
                     /locus_tag="ACUQR0_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73089..73598)
                     /locus_tag="ACUQR0_000235"
     CDS             complement(73089..73598)
                     /locus_tag="ACUQR0_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HHV9107431.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73770..74006
                     /locus_tag="ACUQR0_000236"
     CDS             73770..74006
                     /locus_tag="ACUQR0_000236"
                     /inference="COORDINATES: protein motif:HMM:NF025073.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9107432.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73981..75612
                     /locus_tag="ACUQR0_000237"
                     /pseudo
     CDS             73981..75612
                     /locus_tag="ACUQR0_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT        18610 a        18989 c        19206 g        18811 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11821 atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc tacggcgtct
    11881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    12001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12061 gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12181 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
    12241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca gaagtgggta
    12421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
    12541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gctctttttt
    12961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    13021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat
    13141 agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt
    13201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
    13381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
    13561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
    13741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
    13801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
    13921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
    13981 gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt cgtcgaggga
    14041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
    14101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14821 caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    15001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15241 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
    15301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15361 actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
    15601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
    15781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
    15841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    16021 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16081 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
    16501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16621 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca ttctgctgct
    16861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
    16981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    17041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
    17641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17761 atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact gcggcaaggt
    17821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17941 cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa gccaacctgc
    18001 gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc tcacttattt
    18061 gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt
    18121 tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac tttcctggtc
    18181 ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc
    18241 aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg
    18301 gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc
    18361 gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca
    18421 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18481 accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18541 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18601 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
    18661 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
    18721 ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa
    18781 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggggt
    18841 catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc cttcgaggcg
    18901 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18961 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat agaactggta
    19021 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    19081 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    19141 gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    19201 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19261 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19321 cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc
    19381 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19441 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19501 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19561 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19621 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19681 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19741 cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg ccagcgtact
    19801 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19861 aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    19921 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    19981 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    20041 ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag cggttacatt
    20101 ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg aagcggaatt
    20161 aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat
    20221 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca aattcaatat
    20281 attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag cgtttgaaat
    20341 ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg tagaacttaa
    20401 aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt
    20461 ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc aaactggaat
    20521 gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc
    20581 gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg cacgctgaac
    20641 agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata
    20701 acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta
    20761 cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc cttaatgacg
    20821 taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct
    20881 tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac ctgacggctc
    20941 cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc gccctgatag
    21001 ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc attttcgttg
    21061 agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc
    21121 acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga
    21181 ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc
    21241 aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac
    21301 tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag acgacggttg
    21361 tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcaa
    21421 tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca
    21481 aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc catgtcgatt
    21541 ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc
    21601 gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg
    21661 gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc caggccttta
    21721 ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg
    21781 ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc gacatactcg
    21841 atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc
    21901 tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc
    21961 ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag atcttcatcg
    22021 atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca
    22081 aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta
    22141 gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa aaaaaagcgg
    22201 cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc gcatcaggca
    22261 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg
    22321 agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
    22381 cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct
    22441 tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
    22501 gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt
    22561 ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
    22621 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc
    22681 cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg
    22741 tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag ctgacgcagc
    22801 ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc
    22861 gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag cacccagacg
    22921 tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca gacgggtaat
    22981 acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc
    23041 cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga tctcgtcttc
    23101 tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac
    23161 gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt tctggctggc
    23221 ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag agaagccggc
    23281 atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
    23341 gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
    23401 gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg
    23461 tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
    23521 gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg
    23581 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
    23641 cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
    23701 cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg
    23761 ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg cgatcggcac
    23821 aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt
    23881 ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
    23941 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc
    24001 cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc
    24061 gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
    24121 ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga gctgtaacgc
    24181 cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
    24241 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg atgttctgta
    24301 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca tcgcaaatta
    24361 aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
    24421 ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg cctgtctgcg
    24481 caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt tacttattga
    24541 ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc
    24601 gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg tttgtactgg
    24661 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
    24721 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
    24781 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
    24841 atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc gatgccgagt
    24901 gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg
    24961 taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
    25021 gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
    25081 tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg gatgccgaag
    25141 aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc gccgttgaag
    25201 gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa gcgatcatgg
    25261 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
    25321 tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc cttgctcagg
    25381 gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac
    25441 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
    25501 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
    25561 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac
    25621 cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg
    25681 ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt
    25741 gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga
    25801 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
    25861 tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct
    25921 ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt
    25981 ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat
    26041 tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
    26101 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca
    26161 tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt
    26221 taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
    26281 aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
    26341 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt
    26401 gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac gtctcggcac
    26461 gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta aaatcaccaa
    26521 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
    26581 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
    26641 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc
    26701 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt
    26761 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
    26821 ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct gtttgatctc
    26881 agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg
    26941 gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg caagctgcgc
    27001 gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt
    27061 cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact ggaagaacgt
    27121 tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac
    27181 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg
    27241 gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt
    27301 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
    27361 gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc
    27421 taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa agccgcgaac
    27481 aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct gggtaaaacc
    27541 atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg tgaaatcacc
    27601 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca agcgctgaag
    27661 gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc
    27721 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
    27781 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
    27841 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
    27901 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
    27961 actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg
    28021 tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg
    28081 aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg
    28141 gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca cgttcctaca
    28201 actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca
    28261 ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga
    28321 ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg tggatcatcc
    28381 gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc ggcgataacc
    28441 tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg gtgctttctg
    28501 gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt
    28561 cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg gttggctctg
    28621 atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat
    28681 atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    28741 caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa taaaacccgc
    28801 taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt caccaatcga
    28861 caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca gtgctgcttt
    28921 gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta acggagaagg
    28981 ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga
    29041 aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg
    29101 ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt aacgcggggg
    29161 cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt gaaatctaca
    29221 accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca gggtctgact
    29281 gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg
    29341 gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt ggtcgcgacc
    29401 atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg tatgtggcct
    29461 cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct
    29521 atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg
    29581 atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg gaagattccg
    29641 ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt ggtctggatt
    29701 cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa gatcaggaac
    29761 gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg ggttcaccgg
    29821 atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact
    29881 tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacttatg
    29941 atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcga
    30001 tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt
    30061 acttccataa agcgcccaac gctaaagaac tgcatgaaga gacggtgcgt aaactgctgg
    30121 ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag
    30181 cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg
    30241 ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat
    30301 acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca
    30361 gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag caactggaaa
    30421 ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat ctgtaccgcg
    30481 agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg ggcggtcctt
    30541 ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa aaaatggacg
    30601 atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa atgcggtact
    30661 gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc tgatatgcag
    30721 taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta
    30781 tttcatagat taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa
    30841 agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa tatagcgcca
    30901 cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa aaagaggttg
    30961 acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa
    31021 aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    31081 tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt agacgcacca
    31141 gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg caccattcac
    31201 cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt ttgataccgg
    31261 cattccctgg ttcgaatcca ggtaccccag ccatcttctt cgagtaagcg gttcaccgcc
    31321 cggttattgg ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt
    31381 tcgaatccag gtaccccagc catcgaagaa ataatctggc tacgtagctc agttggttag
    31441 agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accaaattct
    31501 gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat cgccaagcgg
    31561 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccaatt
    31621 tattcaagac gcttacatcg taagtgcacc cagttggggt atcgccaagc ggtaaggcac
    31681 cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac attaaaaaag
    31741 ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc gatgttgaca
    31801 cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct acggctggca
    31861 cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc tgacgtttca
    31921 acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc agcggcggca
    31981 gattattact gaatccggca taaaacagat ccataccact ttgcataatg aagttatccg
    32041 ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct tcaccgtagc
    32101 tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca agattcaccc
    32161 cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca agccccggct
    32221 gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca agcggtgtaa
    32281 cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc gccatattca
    32341 tattctgcaa ctggcgaata cgcgccggag agtcatacca caccagcgat gcccagttat
    32401 caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag gtgctgtcgc
    32461 cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac tgccaggcat
    32521 gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca ccaatcacca
    32581 gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca ttatctcgat
    32641 gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct tccagcgcct
    32701 gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac ggcaacttta
    32761 attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc agtctgcggt
    32821 aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta agcaatgata
    32881 ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg acaaacggtg
    32941 caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc cccagcgcca
    33001 gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga tttgtcatgg
    33061 tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc aagggctgat
    33121 aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg cattcctggc
    33181 tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc tggggctgtc
    33241 agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc cacggctatc
    33301 aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc tcaatccgcg
    33361 agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta aaagagaaga
    33421 atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc gagcacattc
    33481 gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac cgtctgccgg
    33541 agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc ttcccggaaa
    33601 tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg tttgtctcca
    33661 tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc cgtggtgaag
    33721 aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg gctcagggcg
    33781 tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag aactacgacg
    33841 gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac gggatcgatc
    33901 gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc gaagtgtacc
    33961 gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt tccgatcgca
    34021 ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc atccgtaaac
    34081 tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc ttccctggcg
    34141 aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat ttcgacatga
    34201 gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg gttgatgatg
    34261 ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc aatcagcaag
    34321 cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta gaagggactt
    34381 cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg gtcaacttcg
    34441 agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac gtctacccga
    34501 actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc atggcagaaa
    34561 caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg ggttattatc
    34621 agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct ttatccccat
    34681 ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta taggccggtg
    34741 aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag acactcgcga
    34801 aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc cgtttgatga
    34861 caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg ataaccactt
    34921 taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc gtagcctgta
    34981 tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac agatccacct
    35041 tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg agtacggcaa
    35101 agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa accaggcgca
    35161 gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg cgggtacggg
    35221 taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg aaattcgccg
    35281 tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc tgcctggcga
    35341 tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt ttgaaatgct
    35401 gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg cgccgctggc
    35461 ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga gccagaacac
    35521 taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa aagcggttat
    35581 caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct tacgtcatgc
    35641 cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca gcgaagacgt
    35701 ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg aagaagcaga
    35761 acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg aacaaaaatg
    35821 agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt accggaagag
    35881 agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga gtcggaagtg
    35941 actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta tcgcggtaag
    36001 gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat ggagatgtcg
    36061 ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca ggagcaaggc
    36121 aaaccactgg aggcgcattg ggcgcatatg gtggtgcacg gcagtctgca tttgttaggt
    36181 tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac agagattatg
    36241 cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc caggcatttg
    36301 actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac taacatgaga
    36361 acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata agcaacaaga
    36421 agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa aaccgtgacg
    36481 aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa gatacgcgcg
    36541 atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc atgatccccc
    36601 gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt gatgtcatca
    36661 tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac attgaaggga
    36721 ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc ttcagcatgg
    36781 acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac cggatgctga
    36841 aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc ggtggggttt
    36901 ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt gaagacgagt
    36961 atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg accgtgcgcg
    37021 cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc gatgaagaag
    37081 tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg cgtggcgaaa
    37141 ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg cgtattattc
    37201 aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa taaaaccgaa
    37261 actggataga taactacatg gcttttgcct cattaattga acgccagcgt attcgcctgc
    37321 tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac gacgtctggc
    37381 ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt cgcccactcc
    37441 agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt attaactggg
    37501 tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc ttcctggtgg
    37561 tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg ctgtcgcgtc
    37621 tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc tggcaagtga
    37681 ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc ggctatagcc
    37741 agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc attaacttcc
    37801 tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac tggcgtccgc
    37861 tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc caatggttta
    37921 ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt ccgcaatcgc
    37981 tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac gcaacggcac
    38041 cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc gatctggaaa
    38101 ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa ggtagctcgc
    38161 tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat acctacaaca
    38221 ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat cgctataaca
    38281 aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg cgtccgttag
    38341 caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc cagccgccgc
    38401 tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagatcatt ctcgccgagc
    38461 aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc aacgatgcgt
    38521 ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg cgtgcgctgg
    38581 agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg attggcccgc
    38641 agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc actaacgtga
    38701 cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg tgggtgctga
    38761 cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt aaataatccc
    38821 ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc ggcatcagtg
    38881 cattatccgg ataacaatat tctcctatcc aactttggca catctattgc tttgttatac
    38941 aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat ccaccggtag
    39001 caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac ctcgcaaaaa
    39061 caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc taacaggcac
    39121 aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg ggtatccatg
    39181 cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct gccacagcaa
    39241 tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc ccggcagcag
    39301 gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac cgtgaatctt
    39361 cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg caggattact
    39421 ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg caggtaaaac
    39481 tgattccgat tacctcacaa aaccgtattc cactgctgca aaacggcact ttcgattttg
    39541 aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc tctgacacta
    39601 ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa gattttgccg
    39661 acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca
    39721 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
    39781 ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac gctctgctgg
    39841 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc aagccgcagt
    39901 ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa aagctgatgg
    39961 atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataaatggt
    40021 tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga
    40081 aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg
    40141 aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg atggtcgttc
    40201 cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg
    40261 ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg gggtattttt
    40321 ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag tggttttcag
    40381 gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc
    40441 ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa
    40501 ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt gatcccggag
    40561 ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa tattcagttt
    40621 ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta
    40681 cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc gatgggcctg
    40741 acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt tatcgtcccg
    40801 ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc gaccatcggt
    40861 ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca cgcctgggag
    40921 tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat gctggtgatg
    40981 acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata atgtacgagt
    41041 ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcaccc
    41101 tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg ctggcggtga
    41161 tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt aacgtattcc
    41221 gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg ggatttctgc
    41281 aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg atggtggcgt
    41341 tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt caaagtattt
    41401 cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag tcgatgaaac
    41461 tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact cagggcatcg
    41521 tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc ttccgtaccg
    41581 cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt gccggatttg
    41641 tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag
    41701 cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga
    41761 ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggttccgg
    41821 caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag gtgaaatcac
    41881 cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc gctcccgcgt
    41941 cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg aaaacctgac
    42001 cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa aagcattgaa
    42061 attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc agctttccgg
    42121 cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta ttgcgatgct
    42181 gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat
    42241 ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa tgggctttgc
    42301 ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg aagactcgcc
    42361 gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat
    42421 cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc tttatcgttt
    42481 cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca cacaacaagg
    42541 agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg acgctatcgc
    42601 aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg
    42661 aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct tgcttaatcg
    42721 caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt tgattatcgc
    42781 ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac cgacgttcgc
    42841 accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa gtgcggaacc
    42901 tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca atagccatcc
    42961 ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa
    43021 ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt
    43081 ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag cgcaaatcca
    43141 cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga
    43201 actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc
    43261 gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta ccactgttga
    43321 gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg ttgttgatta
    43381 ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg atcgtcaggg
    43441 ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt cattgattaa
    43501 gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt tcaagatata
    43561 aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac caatagttta
    43621 attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg tgaatattta
    43681 acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa accggctgta
    43741 tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc aatgggcagc
    43801 tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt attacgctca
    43861 ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt gatctccgtt
    43921 ccggcttatt tcagtgatga acaacgcaag catacccgtt tagcagcgga cttagccggg
    43981 ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta tggcctgcat
    44041 acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt tgacgttacg
    44101 gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctggcga caacttcctc
    44161 ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc ggatgtcaca
    44221 aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga agcggcaaaa
    44281 tgtagcaatc aatcgccaat gcacattcgc tggtagtacc aggatgaaac gcgggaatgc
    44341 gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg
    44401 ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga cagtctggtg
    44461 ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc
    44521 aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc agcaatccag
    44581 gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga tatttgccct
    44641 tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc gccgattatt
    44701 gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat gcacccggaa
    44761 caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa aaacaacatt
    44821 ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc gattgatatt
    44881 cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga agacggcagc
    44941 gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca gattgaagag
    45001 agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat caatcgcacc
    45061 tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt
    45121 ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc ccgcgtcgat
    45181 gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc acggctgccg
    45241 gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca actaaacaac
    45301 atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat tttgataatg
    45361 ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt ttcaggtgtt
    45421 ggctcatcga catcttcatc ctccggctcg agttttactt tcgtcaacag catccgggtg
    45481 ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag acgggccagc
    45541 atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata caaatccagt
    45601 aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc ccatgctttg
    45661 cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt tccgctatgt
    45721 aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata tttcagcggt
    45781 tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa cgcgaaataa
    45841 tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt agatgggtaa
    45901 ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca gtacaaacgt
    45961 tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg ccactgtttg
    46021 gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg caacagtttg
    46081 cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa aaacgcagtg
    46141 acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc aacagtctgg
    46201 ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc accagcctga
    46261 atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc attaaacgaa
    46321 tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata tcgcgcttct
    46381 atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt ccaggtattt
    46441 aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc tgaatccagt
    46501 aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc ttcatcatct
    46561 gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg gcgtaatgct
    46621 ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag ataagcctgg
    46681 cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca tgttttcata
    46741 ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat ggctgccagg
    46801 gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc cgactcccgg
    46861 cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct tcgcactgag
    46921 aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc cagctgtgat
    46981 agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct tcttccgtca
    47041 gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa tcccaggctt
    47101 tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta taaatcaacc
    47161 ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag cgttgccatg
    47221 tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga taaatagtct
    47281 gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat tcttttatcc
    47341 cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt atttccatgg
    47401 gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa taatccctta
    47461 agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc tttcaaaata
    47521 aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt cgcaattttt
    47581 atagttgcag caattacctg tattagccaa cccaagaaaa cgatgttgcg tgataaagcc
    47641 atggtgaatt atgcctttga ttatttaaga tcaccaggca gtctaccgtt aaccacggca
    47701 gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct tggcgaattt
    47761 tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag
    47821 caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tgaggtggat ctacctgaaa
    47881 ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa
    47941 caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg cactggtatt
    48001 aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa
    48061 aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc acccgaagag
    48121 atggagtatt ttccctatca aaccggcaaa gataaaaatg tagaaaataa ttttgatgac
    48181 gcccagacag cccagcccgc catcatcaac gggcattggc tgtggttgcg gctccggctc
    48241 gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc
    48301 ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc
    48361 tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg
    48421 ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct tcgtgcaaaa ccatctcgac
    48481 cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata aatgaaaacg
    48541 cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctgatcgt taaccagatc
    48601 acgcgcttca atcatcatca ccactaatgg gtaataatag acccgatgct gacggcacca
    48661 ttgcaacatt aaagcatccg cctgggtgga gggatactcg cgccagtagt aacaaagttc
    48721 tgccagcagg ctttcgcctt ctccacaata gacgcgttgt gcgtattcat aataacgcgc
    48781 atcttcattg tcaggttcag cttcccgcca cgaccgggcg taatccagga gttcggggat
    48841 tcccaggcgg cttgcgctat accagcggag taaatttctg tgcagttttg catcatcggg
    48901 gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca
    48961 aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa ctggcagatg
    49021 cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag
    49081 ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa
    49141 taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca
    49201 gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga atcgctgcca
    49261 ggcggaggac agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat
    49321 ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc
    49381 aatccgtagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc
    49441 tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt
    49501 ttcctctata tcgaggatct tccagcaatt tttcataagt cgctcatatc cttaagcgtt
    49561 tccgggcatg tggggtagtc agccagtggt tgccaggaca aggtagggta atacgcatca
    49621 tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct
    49681 aagaattcgc gtaataaagg cagatgatca tcttcgggag actgccaata ttgccgacca
    49741 aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc ccgtagatga
    49801 atacgcgact ggatccatag actttcttct tcactcaacc agttattaag tacgcccatt
    49861 cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc accacagcat
    49921 tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga
    49981 tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc tgccaaccga
    50041 gcggcatgac cgttctcgat cattcggtga attatgcgca gcaggtcatc acggtcatca
    50101 attccccatg aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggggatgaa
    50161 tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt
    50221 ttttgcgacc ccatgtccat ataatttatc cttaaacatt catttaatga atgtaaacta
    50281 catttcgagg ctcagttatt tatacttttc tgacgcaaca taatacgcat aaaataaggc
    50341 ttacgcaaaa atggaataca taaaaatata atatatttta tctacaacca taaggaaatg
    50401 ataatgattt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
    50461 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
    50521 gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa
    50581 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
    50641 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
    50701 gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta taaagaagcg
    50761 gttacctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttgga
    50821 tggatgtaca gaaatggcaa cggagtcgca caagactata cgctggcatt tttctggtac
    50881 aaacaagctg cattacaagg ccatagttac gcacaagaca atctggccga tctttataaa
    50941 gacggagaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
    51001 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
    51061 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
    51121 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
    51181 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
    51241 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
    51301 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
    51361 gagactgaag ctttactgta gaaataaaaa caagggccgg ggaatttcat tctgcccata
    51421 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
    51481 gtcataacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
    51541 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
    51601 atgatgattg aggtcctgga aaatttcgcg ccattcaagc tgcgttttat cggtgatgtc
    51661 tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc
    51721 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt
    51781 cagctcatcg atctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
    51841 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgttca gtgacgcgac
    51901 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
    51961 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
    52021 taatgtacga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
    52081 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
    52141 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
    52201 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
    52261 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
    52321 cacgtacgta actacaccat cggtgacgtg atcgcccgtt accagcgcat gctgggcaaa
    52381 aacgttctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
    52441 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
    52501 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
    52561 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataaa
    52621 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
    52681 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
    52741 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
    52801 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
    52861 atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac
    52921 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
    52981 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
    53041 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
    53101 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
    53161 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
    53221 tacggcctga acatcaaacc ggttatcctg gcggctgacg gctctgagcc agatctttct
    53281 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa cggtcttgac
    53341 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
    53401 aaagtgaact accgcctgcg cgactggggt gtttctcgcc agcgttactg gggcgcgccg
    53461 attccgatgg tgacgctgga agacggtacc gtaatgccaa ccccggacga ccagctgccg
    53521 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
    53581 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
    53641 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
    53701 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
    53761 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
    53821 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
    53881 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
    53941 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
    54001 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
    54061 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
    54121 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
    54181 cgtgtctgga aactggtgta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
    54241 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac tatcgctaaa
    54301 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
    54361 ctgatgaaca aactggcgaa agcaccgacc gatggcgagc aggatcgcgc tctgatgcag
    54421 gaagcactgc aggccgttgt ccgtatgctc aacccgttca ccccgcacat ctgcttcacg
    54481 ctgtggcagg aactcaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
    54541 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
    54601 gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
    54661 gaacatctgg tagcaaaata tcttgatggc gttactgtac gtaaagtgat ttacgtacca
    54721 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
    54781 cactgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgaca
    54841 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
    54901 cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
    54961 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
    55021 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
    55081 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcat
    55141 tcttcgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
    55201 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
    55261 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
    55321 gcgtctccac catgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
    55381 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
    55441 ggaaagccag gacgctgttc gccaggtagc tgcggcacaa ggattcgaag aacaccacac
    55501 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
    55561 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
    55621 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tattgatcgt
    55681 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
    55741 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
    55801 tgtgcgcgca aaacagctca acttagaact ggatgacgcg gcgaatcagg tgctctgcta
    55861 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
    55921 gccagacggc aaactgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
    55981 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat
    56041 tcttcagcaa ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca cattacaacg
    56101 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
    56161 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
    56221 cttaagtcag ccgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
    56281 caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc ttctgttgtg
    56341 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
    56401 ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa acgctggcga
    56461 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
    56521 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
    56581 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
    56641 cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt attattggtc
    56701 aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
    56761 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
    56821 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
    56881 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
    56941 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtattgact tacattaacc
    57001 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
    57061 tcgtcatggc gaaacacaag cgaatgtcga tggtctgtac agcggtcatg cgcccacacc
    57121 cctgaccgca cgcggtattg agcaagcgca aaatctgcat acactgctag atgatgtttc
    57181 ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc gactggttct
    57241 cagtgaccgc cagctccccg ttcatatcat ccctgaactc aacgaaatgt tttttggcga
    57301 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
    57361 gtgcaatgac tggcagcatg ctatccccac gaatggtgaa ggatttcagg cattttcgca
    57421 acgtgtggaa cgctttatcg caaggcttag tgaatatcag cactatcaga atattttaat
    57481 cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ttaattggca tgcctgccga
    57541 gtctatgtgg cattttcgcg ttgaccaggg gtgttggagc gccattgata tcaaccaaaa
    57601 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaacg catgactttt
    57661 ctgttttttt acgggtaagc cgcaacgacc attgacagcc ccgggcaggc tgatattctc
    57721 cgcagccaga ctttttccgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
    57781 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
    57841 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
    57901 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
    57961 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
    58021 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
    58081 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
    58141 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
    58201 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
    58261 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
    58321 aagatatgcc cttcgagctg attgaaattc cggccggcaa acgcggcaag aatgcggaca
    58381 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
    58441 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
    58501 aacgctggaa gctggatggt cgcgacgtaa gtctactgat tggcgggcct gaagggttgt
    58561 cgcctgcctg taaagcggcg gctgagcaga gctggtcgct gtcggcgctt accctccccc
    58621 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
    58681 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
    58741 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
    58801 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
    58861 aaattgttcg ctttaccgac taccagaccc gctctaatga aaaccgcatt aagctggtgc
    58921 ctatcgcgcc cagccgcggc attatctacg accgtaacgg tatccctctg gccctcaacc
    58981 gcactatcta ccagatagaa atgatgccgg agaaagtcga taacgtgcag caaacgctgg
    59041 acgctttgcg cagcgtggta gatctgaccg atgacgatat tgctgcattc cgaaaagagc
    59101 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctaacc gaagtacaag
    59161 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
    59221 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
    59281 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
    59341 atgatattgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
    59401 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
    59461 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
    59521 aaacgctgct ggcgggtagc cgcgcagctg tggttgttac cgaaccgcgt acaggtgggg
    59581 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
    59641 gcaaagatta ttccgccttg ctgaacgacc cgaatacacc gctggtgaac cgcgccacac
    59701 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattaagcg
    59761 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctaccagggt
    59821 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
    59881 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
    59941 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
    60001 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
    60061 ggtatcaggg tgacaccatt ccggttggta tcggccaggg ttactggaca gcgaccccaa
    60121 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttccgcatt
    60181 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacaaccg catgaaccgc
    60241 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
    60301 ctaaccgccc taatggtacg gcgaataaat actttgccag cgcaccgtac aaaattgcgg
    60361 cgaaatctgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    60421 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    60481 cgcaagtggc tgtcgccatg attctggaga acggcggcgc gggtccggcg gttggtacgc
    60541 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    60601 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    60661 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgattttac tggcattgct
    60721 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    60781 taaaatcggc caaatcgcga tgggtctggt catcatggtg gtgatggcgc aaattcctcc
    60841 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    60901 ggtagatgct ttcggagcca tctctaaagg cgctcaacgc tggctggacc tcggtattgt
    60961 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    61021 caaccgcgac gtttgcccgc catcgttgaa gaatactggc atcgcgctgg tgctgatatt
    61081 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    61141 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    61201 agtgctggta gcggcgttca ttccgattct gtggttcttc ctgatgcatg attaccagcg
    61261 ccagcgtgta atgatgctcc tggacccgga atcagaccca ctcggcgcgg gctatcacat
    61321 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    61381 cactcagtca cagcttgaat ttctccccga acgccatact gactttatct tcgcggtact
    61441 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    61501 catgcgcggg atgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    61561 tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    61621 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    61681 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    61741 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    61801 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    61861 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    61921 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    61981 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaacca ggcagtaacc
    62041 tgacggcctc tggcgaagct ttcgatccga caaagctgac ggcggcccat ccaacgcttc
    62101 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    62161 ttaatgatcg cggcccttac ggcaacgacc gcgttatttc gctttctcgc gcggcagctg
    62221 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    62281 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    62341 tgcctgcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg
    62401 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    62461 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctagtgta ctggaaggca
    62521 gcgaaccgac gcctgcgcca cagcccgttg ttacagcttc gtcgacaacg cctgcaacct
    62581 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    62641 aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    62701 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    62761 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    62821 tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    62881 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    62941 tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    63001 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    63061 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    63121 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    63181 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    63241 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    63301 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    63361 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    63421 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    63481 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    63541 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    63601 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    63661 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    63721 acactgacaa agcaggttac aatcttgttg cttctgcgac tgaaggccag atgcgcttga
    63781 tttctgcggt gatgggtgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    63841 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    63901 cctctgaacc ggtttggttt agtgattctg atcgcgcttc gttaggggtt gataaagacg
    63961 tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    64021 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    64081 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    64141 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    64201 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaaactc
    64261 ccgccttctg gcgggagttg ctatttaatt acgttacgcc ggagctgaca tgaaaaccaa
    64321 acttaacgaa ctgcttgaat tccctactcc ttttacttac aaagttatgg ggcaggcgtt
    64381 acctgagctg gttgatcagg tggttgaagt ggtacagcgc catgcgccag gtgactacac
    64441 cccaacggta aaaccaagca gcaaaggcaa ctaccactcg gtatctatca ctatcaacgc
    64501 cactcatatc gagcaggttg aaacactgta tgaagaactg ggcaaaatcg atattgtccg
    64561 catggttctg taactcgctt ctccgttacc cggctcctgg tcgggtaact cccctctcct
    64621 cgctgtgata tacttttccc ccacttttac tcattctcca cggagatgcc gttttgtatc
    64681 aggataaaat tcttgtccgc cagctcggtc ttcagcctta cgagccaatc tcccgggcta
    64741 tgcatgaatt caccgatacc cgcgataata gtacccttga tgaaatctgg ctggtcgagc
    64801 actatccggt attcacccaa ggtcaggcag gaaaagcgga gcacatttta atgccgggtg
    64861 atattccggt gatccagagc gatcgcggtg ggcaggtgac ttatcacggg ccgggacaac
    64921 aggtgatgta tgtgttgctt aacctgaaac gccgtaaact cggtgtgcgt gaactggtga
    64981 ccttgcttga gcaaacagtg gtgaataccc tggctgaact gggtatagaa gcgcatcctc
    65041 gggctgacgc gcctggtgtc tatgtcgggg aaaagaaaat ttgctcactg ggtttacgaa
    65101 ttcgacgcgg ttgttcattc cacggtctgg cattaaacgt caatatggat ctttcaccat
    65161 ttttacgtat taatccttgt gggtatgccg gaatggaaat ggctaaaata tcacaatgga
    65221 aacccgaagc gacgactaat aatattgctc cacgtttact ggaaaatatt ttagcgctac
    65281 taaacaatcc ggacttcgaa tatattaccg cttaattcca tacatcaatg gcccaattta
    65341 cactgggtca ttactcataa tacctgctaa tgctttacat ctccttcgcg tttgtatatt
    65401 ttctttatca cttgcagaaa tgcggtgcca cgccttgctc cgtaactcac cccattgatt
    65461 aacctattga aatagttgat tatggtgagc aaaagcgttc cgttccaatt agacataaga
    65521 ttattgtaat tttttaaata ttattttccc atgaataaaa ttggagtaag tgcgtggata
    65581 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    65641 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    65701 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    65761 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    65821 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    65881 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    65941 ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaaactcact
    66001 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    66061 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    66121 ttctgttcac cgacaatatg gtgttaattt gccgcaaggg aaatccacta ctctctttag
    66181 aagatgacag agagactatc gacaacgctg cgcatgtact tctgttaccg gaagggcaaa
    66241 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcaatttca
    66301 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    66361 ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc
    66421 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc atcctgcgtg
    66481 atcccatcct gcacggagta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    66541 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    66601 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    66661 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    66721 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    66781 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    66841 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    66901 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    66961 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    67021 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    67081 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    67141 gttgaccgtg atgacctgcg cgatggcggt gcccaacact ttgcggattg tattactgcc
    67201 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgcggtcga
    67261 atggatcgtg ctctggatat tctgactgca acgcccccag atgtgttcaa ccataacctg
    67321 gaaaacgtac cgcgtattta ccggcaggta cggcctggcg cagattacaa ctggtcgctg
    67381 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    67441 gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    67501 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    67561 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    67621 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    67681 aaaggaatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    67741 gctttttgaa gcgggttttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac
    67801 gtcactcttt atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg
    67861 cagcatcgtc atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc
    67921 ccagcgtacg taacttctta gtcccaaaca gcagaacgac cagcgccgca actaccagca
    67981 gtttggtaat actaatctca cccatagata ccttcttgac atataaccgg caacagaacg
    68041 cgccttaaaa accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt
    68101 aacaaggtga atcaagcgcc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg
    68161 acggttgttt aagatgggca gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc
    68221 cgccataatg agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat
    68281 aatccggctt tgcccgatat ttttgttccc gcactccccc gccgccacca tataacaggt
    68341 ggtatccagc gcacgagcgg caagcaacgt tgaccagtga tgctctttga gcggcccacg
    68401 aacccaggcg gcaggaagta ccaggatttc agctccctgt aaagcctgtg ccagcgccag
    68461 ctctggaaag cgtaagtcat aacaggtcat cagaccgacc ttcatccctt ccacctccag
    68521 taacggagct atttcattac cagcatcaac atggcgtgat tcctgaatgg caaatgcatc
    68581 atagagatgc agtttggcat aacgggcgac gatgtttcct gcctgaagtg ccaccagcat
    68641 attccatgcg cgccccggcg ttgaaggaag atgaatcgtc agaattgtcg tcatcatgtt
    68701 acgtttactt tctcgccgta aacgtccgag gaattcgcct tccagcagct gtgctgattt
    68761 aaccgataga tctgcatcat gatcatcgcg cgccagcaat gcttccggca ggacaaacag
    68821 cgatacgtcg ttttccgccg cctgcgccat caacgaggca caaatctcag cgttcttttc
    68881 ccacacagat gtaacagcaa actgtcctgc agcaaccagc attgccacct cctgtcagag
    68941 tcgtaaaatt cgccaacgac gattattcgg cgttacactt atcactcata caaatcaaat
    69001 agcaggattt tgcagtgtta caacttcttt tagcagtttt tattggcggt ggtacgggaa
    69061 gcgtagcgag atggctgtta agtatgcgat ttaacccgct gcatcaggcg attccgttgg
    69121 ggacgctggc agcaaacctg attggggcat tcatcatagg aatgggattc gcctggttca
    69181 gcaggatgac gaacattgat ccagtgtgga aagtattaat caccaccgga ttttgtggcg
    69241 gtctaacaac cttctcaaca ttttcggcag aagtggtgtt tttgttacaa gagggccgct
    69301 ttggctgggc attactgaac gttttcgtca accttctggg gtcttttgcc atgaccgcac
    69361 tggcattctg gctgttttcg gcctcaaccg tacactaaag accaaaaaaa acccgctgat
    69421 taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag
    69481 ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa
    69541 aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag
    69601 taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca
    69661 tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttatc agtacaccaa
    69721 ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt
    69781 gatgttgctt attttagtta ttgattttaa agaagttact aaaacttcat ttgtctcaaa
    69841 actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg
    69901 tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag
    69961 ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa
    70021 aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc
    70081 atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat
    70141 cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt
    70201 ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa tcataatgtt
    70261 caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatcctc tcatctgcgt
    70321 tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag atagcgacat
    70381 atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc
    70441 aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg aatagctgct
    70501 ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag atgatgcgtc
    70561 gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt cttttttgat gacaaaatgc
    70621 aatcaaggaa aaattatttt atttttttaa cgagatacaa caatcatctt aacgaagtat
    70681 ataatatata tcgtcaacca tgctcatatt agattatttt ttgagcgcac agccgcagct
    70741 aacaccattg caattaacaa atttgcatca atccaccatc aatttacaca cattattatg
    70801 tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac agcaacttca
    70861 atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt tattgggcat
    70921 attttttcct taagctttag gaagttttta ttatttactt tgggcctgga gacaggaaaa
    70981 attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg tgtggctcgc
    71041 tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg
    71101 attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac
    71161 agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc
    71221 ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat cggcgcgaat
    71281 gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg
    71341 atgattgccg cctattttgt tgcttgtctg atgtcactgg ccgtctcttc cgcaaccggt
    71401 ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt
    71461 ggcgcagcag ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg
    71521 gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg
    71581 acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa
    71641 cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc
    71701 accactgccc ccgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt
    71761 tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg
    71821 attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg
    71881 gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc
    71941 gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc
    72001 gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgaccatg
    72061 ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc
    72121 ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag
    72181 gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg
    72241 atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg
    72301 ctagtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca
    72361 gacaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt cacccactgt
    72421 ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc
    72481 ggctggcgca atattcaaga taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg
    72541 ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc accgcgttta
    72601 gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga gcataggcat
    72661 taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac
    72721 gctggcggaa acgggttagc gtttgcccca gtgtattgat tcacttgaag tacgaaaaaa
    72781 ccgggagaac attggattat tcgggatctg atgggattag atttggtggg gcttgcaagc
    72841 ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa agatagtaaa aaattgcttt
    72901 tgtttcattg aaaaaacgaa aaacaaaaac actataaatc atttcaatca tagcttcaaa
    72961 aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca caaatcagct
    73021 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggct
    73081 catggttgtt atacggcttt tttcgcatct tgtggttcca acaaaggagc tacaggctct
    73141 tcctgtttat gaacaccata cattgacaaa atctcttggc gagtttctgg aggtaattta
    73201 tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac ccagcgttgc caatagggtg
    73261 tcaacggaga tggaggggcg ttcttgcatt aatccggaaa gcccatattt agccatgttt
    73321 agctctatct ctcggacttc attttctcct agtttattga ttaacaggga gaacattttc
    73381 aatgtatcag cattcatttc ttcgatagca attttttgca tagccggtat tgccgggata
    73441 tattcaaccc caggcccgcg acgcccttct tgtgaacgct tcggccatcc ttctttcttt
    73501 gctcgtttat gcaccccctg tattgtccca ggcattccat ccagttcagt aatttgtttg
    73561 ggtgtcatcc actggtttat ctcatttgcg cttttcataa actaagttta ccagatttat
    73621 ctatataaaa atcaatggat tacaaaataa accacattaa ctgatataaa ccggttttgt
    73681 ttatttggta atgtataaaa cagataatca agacgaatta tttgttttgg taacccttta
    73741 tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga ttggcatcgg gccgacgtga
    73801 ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt gtccagacgt tcaggttatt
    73861 caaccgggac gcttgcaaat gcattagtca ggccatggcc taaaggtgaa cgcattattg
    73921 ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag tagatatcta agggatcaca
    73981 atgagagagt ggatatcagc aattgaatgc atgggtttat ctggtttacc tggttcatta
    74041 atgggggttc acaaaagagc tcagagagaa ggatggctca agagaagaag agaaggaatt
    74101 agaggggttg cttacgaata ccatatatct tctcttccta acattgctcg agctgaagtt
    74161 ttgctccaac aaggaaaatt taaaaccagt cagggatgtt ttgaaatcgc ccgccccacg
    74221 ctggaagctc atgattatga tcgtgaggca ctatggagca aatgggataa agccagcgat
    74281 tcccagcgca gcctggctga aaaatggttg ccctcaattc aggccacaga cgaaatgctg
    74341 aaccagggga tttcaacgaa aacggctttt gcgaccgttg cagggcatta ccaggtcagc
    74401 gcatccactt tgcgggacaa gtattaccag gtacagaagt ttgcgaagcc tgactgggcg
    74461 gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg ttcacaaaag tgaatttgac
    74521 gaggatgcct ggcagtttct gattgcagat tatctgcgac cggaaaaacc cgccttccgc
    74581 aaatgttatg agcgtctgga actggcagcc cgcgagcatg gctggagtat tccctcccgt
    74641 gccacggcct ttcgccggat tcagcaactg gacgaggcaa tggttgttgc ctgtcgtgaa
    74701 ggtgaacatg cactgatgca tctgataccg gcacagcagc gaactgtgga acacctggac
    74761 gccatgcagt ggatcaacgg cgacggttat ctgcataacg tctttgtacg ctggtttaac
    74821 ggtgatgtga tccgtccgaa aacatggttc tggcaggatg tgaaaacccg aaaaattctg
    74881 ggctggcgct gcgatgtgag cgagaacatt gattcaattc gcctctcgtt catggatgtt
    74941 gtgactcgct acagtatccc ggaggatttt cacatcacca ttgataacac ccgtggtgct
    75001 gcgaataaat ggctgacggg aggcgcgccc aatcgctacc gctttaaggt aaaagaggac
    75061 gatccaaaag gactgttttt actgatgggt gcgaaaatgc actggacaag cgttgttgcc
    75121 ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt tcggtgttgg tgggcttgag
    75181 gaatacgttg ataagcatcc ggcactggct ggcgcatata cggggccaaa tccgcaggca
    75241 aaacctgata actatggcga ccacgctgtt gatgcagagc tgtttctgaa aacctttgcc
    75301 gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa cagaaatgtg cgggggcaaa
    75361 ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa cgattgtgcg taagccaacc
    75421 gaagaacaaa aacggatgct gttactgcct gccgaggcgg tgaacgtttc acgcaaaggc
    75481 gagtttacgc ttaaagttgg cggctccctt aaaggcgcga aaaacgttta ttacaacatg
    75541 gcattaatga atgccggagt gaaaaaagtt gtggtcagat ttgatccaca gcagttacac
    75601 agcacggttt attgag
//
LOCUS       DBKELA010000004        68342 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.004, whole genome shotgun sequence.
ACCESSION   DBKELA010000004 DBKELA010000000
VERSION     DBKELA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.004"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..446)
                     /gene="rsmI"
                     /locus_tag="ACUQR0_000238"
     CDS             complement(<1..446)
                     /gene="rsmI"
                     /locus_tag="ACUQR0_000238"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HHV9107433.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKS"
     gene            511..2547
                     /gene="lpoA"
                     /locus_tag="ACUQR0_000239"
     CDS             511..2547
                     /gene="lpoA"
                     /locus_tag="ACUQR0_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HHV9107434.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            2505..2900
                     /locus_tag="ACUQR0_000240"
     CDS             2505..2900
                     /locus_tag="ACUQR0_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HHV9107435.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            2920..3510
                     /gene="diaA"
                     /locus_tag="ACUQR0_000241"
     CDS             2920..3510
                     /gene="diaA"
                     /locus_tag="ACUQR0_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HHV9107436.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            3520..4095
                     /gene="dolP"
                     /locus_tag="ACUQR0_000242"
     CDS             3520..4095
                     /gene="dolP"
                     /locus_tag="ACUQR0_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HHV9107437.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4209..5249)
                     /locus_tag="ACUQR0_000243"
     CDS             complement(4209..5249)
                     /locus_tag="ACUQR0_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHV9107438.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5322..5957)
                     /locus_tag="ACUQR0_000244"
     CDS             complement(5322..5957)
                     /locus_tag="ACUQR0_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HHV9107439.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6085..6603
                     /gene="yhbO"
                     /locus_tag="ACUQR0_000245"
     CDS             6085..6603
                     /gene="yhbO"
                     /locus_tag="ACUQR0_000245"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HHV9107440.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(6583..7026)
                     /locus_tag="ACUQR0_000246"
     CDS             complement(6583..7026)
                     /locus_tag="ACUQR0_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HHV9107441.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7077..7379
                     /gene="yhbQ"
                     /locus_tag="ACUQR0_000247"
     CDS             7077..7379
                     /gene="yhbQ"
                     /locus_tag="ACUQR0_000247"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HHV9107442.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(7366..7869)
                     /locus_tag="ACUQR0_000248"
     CDS             complement(7366..7869)
                     /locus_tag="ACUQR0_000248"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9107443.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(7863..8387)
                     /gene="ubiT"
                     /locus_tag="ACUQR0_000249"
     CDS             complement(7863..8387)
                     /gene="ubiT"
                     /locus_tag="ACUQR0_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis accessory
                     factor UbiT"
                     /protein_id="HHV9107444.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            8596..9591
                     /gene="ubiU"
                     /locus_tag="ACUQR0_000250"
     CDS             8596..9591
                     /gene="ubiU"
                     /locus_tag="ACUQR0_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiU"
                     /protein_id="HHV9107445.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTSLGAYHRKWQ"
     gene            9600..10478
                     /locus_tag="ACUQR0_000251"
     CDS             9600..10478
                     /locus_tag="ACUQR0_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HHV9107446.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            10559..11566
                     /locus_tag="ACUQR0_000252"
     CDS             10559..11566
                     /locus_tag="ACUQR0_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HHV9107447.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(11684..12928)
                     /gene="mtr"
                     /locus_tag="ACUQR0_000253"
     CDS             complement(11684..12928)
                     /gene="mtr"
                     /locus_tag="ACUQR0_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HHV9107448.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13082..14971)
                     /gene="deaD"
                     /locus_tag="ACUQR0_000254"
     CDS             complement(13082..14971)
                     /gene="deaD"
                     /locus_tag="ACUQR0_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HHV9107449.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(14964..15044)
                     /gene="yrbN"
                     /locus_tag="ACUQR0_000255"
     CDS             complement(14964..15044)
                     /gene="yrbN"
                     /locus_tag="ACUQR0_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HHV9107450.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15151..16035)
                     /gene="nlpI"
                     /locus_tag="ACUQR0_000256"
     CDS             complement(15151..16035)
                     /gene="nlpI"
                     /locus_tag="ACUQR0_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HHV9107451.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16144..18279)
                     /gene="pnp"
                     /locus_tag="ACUQR0_000257"
     CDS             complement(16144..18279)
                     /gene="pnp"
                     /locus_tag="ACUQR0_000257"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HHV9107452.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(18526..18795)
                     /gene="rpsO"
                     /locus_tag="ACUQR0_000258"
     CDS             complement(18526..18795)
                     /gene="rpsO"
                     /locus_tag="ACUQR0_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HHV9107453.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(18944..19888)
                     /gene="truB"
                     /locus_tag="ACUQR0_000259"
     CDS             complement(18944..19888)
                     /gene="truB"
                     /locus_tag="ACUQR0_000259"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HHV9107454.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(19888..20289)
                     /gene="rbfA"
                     /locus_tag="ACUQR0_000260"
     CDS             complement(19888..20289)
                     /gene="rbfA"
                     /locus_tag="ACUQR0_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HHV9107455.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(20453..23101)
                     /gene="infB"
                     /locus_tag="ACUQR0_000261"
     CDS             complement(20453..23101)
                     /gene="infB"
                     /locus_tag="ACUQR0_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HHV9107456.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23126..24613)
                     /gene="nusA"
                     /locus_tag="ACUQR0_000262"
     CDS             complement(23126..24613)
                     /gene="nusA"
                     /locus_tag="ACUQR0_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HHV9107457.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(24641..25093)
                     /gene="rimP"
                     /locus_tag="ACUQR0_000263"
     CDS             complement(24641..25093)
                     /gene="rimP"
                     /locus_tag="ACUQR0_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HHV9107458.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25300..25376)
                     /locus_tag="ACUQR0_000264"
     tRNA            complement(25300..25376)
                     /locus_tag="ACUQR0_000264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25340..25342),aa:Met,seq:cat)
     gene            25724..27067
                     /gene="argG"
                     /locus_tag="ACUQR0_000265"
     CDS             25724..27067
                     /gene="argG"
                     /locus_tag="ACUQR0_000265"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HHV9107459.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27075..28701)
                     /locus_tag="ACUQR0_000266"
                     /pseudo
     CDS             complement(27075..28701)
                     /locus_tag="ACUQR0_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(29160..29246)
                     /locus_tag="ACUQR0_000267"
     tRNA            complement(29160..29246)
                     /locus_tag="ACUQR0_000267"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29210..29212),aa:Leu,seq:gag)
     gene            complement(29261..29593)
                     /gene="secG"
                     /locus_tag="ACUQR0_000268"
     CDS             complement(29261..29593)
                     /gene="secG"
                     /locus_tag="ACUQR0_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HHV9107460.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(29821..31158)
                     /gene="glmM"
                     /locus_tag="ACUQR0_000269"
     CDS             complement(29821..31158)
                     /gene="glmM"
                     /locus_tag="ACUQR0_000269"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HHV9107461.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31151..31999)
                     /gene="folP"
                     /locus_tag="ACUQR0_000270"
     CDS             complement(31151..31999)
                     /gene="folP"
                     /locus_tag="ACUQR0_000270"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HHV9107462.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32089..34023)
                     /gene="ftsH"
                     /locus_tag="ACUQR0_000271"
     CDS             complement(32089..34023)
                     /gene="ftsH"
                     /locus_tag="ACUQR0_000271"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HHV9107463.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34123..34752)
                     /gene="rlmE"
                     /locus_tag="ACUQR0_000272"
     CDS             complement(34123..34752)
                     /gene="rlmE"
                     /locus_tag="ACUQR0_000272"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HHV9107464.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34878..35171
                     /gene="yhbY"
                     /locus_tag="ACUQR0_000273"
     CDS             34878..35171
                     /gene="yhbY"
                     /locus_tag="ACUQR0_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HHV9107465.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35327..35803)
                     /gene="greA"
                     /locus_tag="ACUQR0_000274"
     CDS             complement(35327..35803)
                     /gene="greA"
                     /locus_tag="ACUQR0_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HHV9107466.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36051..37484
                     /gene="dacB"
                     /locus_tag="ACUQR0_000275"
     CDS             36051..37484
                     /gene="dacB"
                     /locus_tag="ACUQR0_000275"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HHV9107467.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(37670..38842)
                     /gene="cgtA"
                     /locus_tag="ACUQR0_000276"
     CDS             complement(37670..38842)
                     /gene="cgtA"
                     /locus_tag="ACUQR0_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HHV9107468.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(38858..39823)
                     /locus_tag="ACUQR0_000277"
     CDS             complement(38858..39823)
                     /locus_tag="ACUQR0_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHV9107469.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(39950..40207)
                     /gene="rpmA"
                     /locus_tag="ACUQR0_000278"
     CDS             complement(39950..40207)
                     /gene="rpmA"
                     /locus_tag="ACUQR0_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HHV9107470.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40228..40539)
                     /gene="rplU"
                     /locus_tag="ACUQR0_000279"
     CDS             complement(40228..40539)
                     /gene="rplU"
                     /locus_tag="ACUQR0_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HHV9107471.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            40797..41768
                     /gene="ispB"
                     /locus_tag="ACUQR0_000280"
     CDS             40797..41768
                     /gene="ispB"
                     /locus_tag="ACUQR0_000280"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HHV9107472.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            41995..42273
                     /gene="sfsB"
                     /locus_tag="ACUQR0_000281"
     CDS             41995..42273
                     /gene="sfsB"
                     /locus_tag="ACUQR0_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HHV9107473.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(42321..43580)
                     /gene="murA"
                     /locus_tag="ACUQR0_000282"
     CDS             complement(42321..43580)
                     /gene="murA"
                     /locus_tag="ACUQR0_000282"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HHV9107474.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(43635..43889)
                     /gene="ibaG"
                     /locus_tag="ACUQR0_000283"
     CDS             complement(43635..43889)
                     /gene="ibaG"
                     /locus_tag="ACUQR0_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HHV9107475.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44049..44342)
                     /gene="mlaB"
                     /locus_tag="ACUQR0_000284"
     CDS             complement(44049..44342)
                     /gene="mlaB"
                     /locus_tag="ACUQR0_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HHV9107476.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44342..44977)
                     /gene="mlaC"
                     /locus_tag="ACUQR0_000285"
     CDS             complement(44342..44977)
                     /gene="mlaC"
                     /locus_tag="ACUQR0_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HHV9107477.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(44996..45547)
                     /gene="mlaD"
                     /locus_tag="ACUQR0_000286"
     CDS             complement(44996..45547)
                     /gene="mlaD"
                     /locus_tag="ACUQR0_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HHV9107478.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(45552..46334)
                     /gene="mlaE"
                     /locus_tag="ACUQR0_000287"
     CDS             complement(45552..46334)
                     /gene="mlaE"
                     /locus_tag="ACUQR0_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HHV9107479.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46342..47151)
                     /gene="mlaF"
                     /locus_tag="ACUQR0_000288"
     CDS             complement(46342..47151)
                     /gene="mlaF"
                     /locus_tag="ACUQR0_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HHV9107480.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            47361..48326
                     /locus_tag="ACUQR0_000289"
     CDS             47361..48326
                     /locus_tag="ACUQR0_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HHV9107481.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            48340..49326
                     /gene="kdsD"
                     /locus_tag="ACUQR0_000290"
     CDS             48340..49326
                     /gene="kdsD"
                     /locus_tag="ACUQR0_000290"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HHV9107482.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49347..49913
                     /gene="kdsC"
                     /locus_tag="ACUQR0_000291"
     CDS             49347..49913
                     /gene="kdsC"
                     /locus_tag="ACUQR0_000291"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HHV9107483.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            49910..50485
                     /gene="lptC"
                     /locus_tag="ACUQR0_000292"
     CDS             49910..50485
                     /gene="lptC"
                     /locus_tag="ACUQR0_000292"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HHV9107484.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            50454..51011
                     /gene="lptA"
                     /locus_tag="ACUQR0_000293"
     CDS             50454..51011
                     /gene="lptA"
                     /locus_tag="ACUQR0_000293"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HHV9107485.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51018..51743
                     /gene="lptB"
                     /locus_tag="ACUQR0_000294"
     CDS             51018..51743
                     /gene="lptB"
                     /locus_tag="ACUQR0_000294"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HHV9107486.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            51791..53224
                     /gene="rpoN"
                     /locus_tag="ACUQR0_000295"
     CDS             51791..53224
                     /gene="rpoN"
                     /locus_tag="ACUQR0_000295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HHV9107487.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53247..53534
                     /gene="hpf"
                     /locus_tag="ACUQR0_000296"
     CDS             53247..53534
                     /gene="hpf"
                     /locus_tag="ACUQR0_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HHV9107488.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            53652..54143
                     /gene="ptsN"
                     /locus_tag="ACUQR0_000297"
     CDS             53652..54143
                     /gene="ptsN"
                     /locus_tag="ACUQR0_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HHV9107489.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54189..55043
                     /gene="rapZ"
                     /locus_tag="ACUQR0_000298"
     CDS             54189..55043
                     /gene="rapZ"
                     /locus_tag="ACUQR0_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HHV9107490.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55040..55312
                     /gene="npr"
                     /locus_tag="ACUQR0_000299"
     CDS             55040..55312
                     /gene="npr"
                     /locus_tag="ACUQR0_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HHV9107491.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            55525..56151
                     /gene="yrbL"
                     /locus_tag="ACUQR0_000300"
     CDS             55525..56151
                     /gene="yrbL"
                     /locus_tag="ACUQR0_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HHV9107492.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56148..56875)
                     /gene="mtgA"
                     /locus_tag="ACUQR0_000301"
                     /pseudo
     CDS             complement(56148..56875)
                     /gene="mtgA"
                     /locus_tag="ACUQR0_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(56872..57525)
                     /gene="elbB"
                     /locus_tag="ACUQR0_000302"
     CDS             complement(56872..57525)
                     /gene="elbB"
                     /locus_tag="ACUQR0_000302"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HHV9107493.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(57755..60091)
                     /gene="arcB"
                     /locus_tag="ACUQR0_000303"
     CDS             complement(57755..60091)
                     /gene="arcB"
                     /locus_tag="ACUQR0_000303"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HHV9107494.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60187..61116)
                     /locus_tag="ACUQR0_000304"
     CDS             complement(60187..61116)
                     /locus_tag="ACUQR0_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HHV9107495.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            61791..66251
                     /gene="gltB"
                     /locus_tag="ACUQR0_000305"
     CDS             61791..66251
                     /gene="gltB"
                     /locus_tag="ACUQR0_000305"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HHV9107496.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66264..67682
                     /gene="gltD"
                     /locus_tag="ACUQR0_000306"
     CDS             66264..67682
                     /gene="gltD"
                     /locus_tag="ACUQR0_000306"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HHV9107497.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            67866..>68342
                     /locus_tag="ACUQR0_000307"
     CDS             67866..>68342
                     /locus_tag="ACUQR0_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HHV9107498.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16708 a        18572 c        16982 g        16080 t
ORIGIN      
        1 gatttggcag gtaaaaagcc ttcgtaacag aaacggtcag atggtaaacc cgcggcgctt
       61 aacgcagtga tagcagcaca cggtccgggt agcggcacca cacgaatccc cgcttcacgg
      121 caggtacgca ccagatggta gccaggatcg ttaattagcg gcgttccggc atcggaaacc
      181 agcgcaatgt tttgcccctc ttgcagcttt gccagcagcg tttcggcttt ttgttgttcg
      241 ttatggtcgt gcagcgcaaa caaccgggca ttaatcccaa aatgttgcag caataaaccg
      301 gtgtgacgag tatcctcggc ggcaatcaga tcaacggcct gtaatacctc taacgtacgc
      361 tgggtgatat ccgccagatt gccgattggc gtcggtacaa tgtaaagctg gccctgagaa
      421 ttatccgccg attggtgttg tttcattgtg tcgtccgtat tgccgattta atattgagca
      481 ttgcgtaaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
      541 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
      601 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
      661 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
      721 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
      781 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggcaga gattaaactg
      841 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
      901 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
      961 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgcttggcgc gaaagaaaaa
     1021 cagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     1081 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     1141 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcagaaa
     1201 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     1261 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     1321 gtatttggtc gcactattca gcaaggcttt gaagccgcga aaaatatcgg cactcagcca
     1381 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag cagaacaacc tcagccgcaa
     1441 actgcggata gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     1501 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     1561 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     1621 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     1681 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tactggcact gaaccagccg
     1741 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcaccgga agacgaagcg
     1801 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     1861 cgcagtgcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     1921 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     1981 ggcggttctg gtattgcttt aacgggtagc ccgattactc ccagagcgac aaccgactcc
     2041 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     2101 aatggcggtc gtgtcgatgc ggtctacatt gtggcaacgc cgggtgaaat cgcttttatc
     2161 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caacgctgta cgccagttcc
     2221 cgcagcgcac aaggcaccgc aggcccggat ttccgactgg agatggaagg cttgcagtac
     2281 agcgaaatcc cgatgctggc aggcggtaat ctgccgttaa tgcagcaggc gctcagcgcg
     2341 gtgaacaacg attattcgct ggctcgcatg tatgcgatgg gcgttgatgc ctggtcgttg
     2401 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     2461 agtctgacgg ctaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     2521 caaggtcagg tagtccccgc cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     2581 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     2641 gtggcggcga gatcgatctg ataatgcgtg aaggcctgac caccgttttt gtcgaggtac
     2701 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     2761 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     2821 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     2881 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     2941 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     3001 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     3061 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     3121 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     3181 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     3241 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     3301 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     3361 ggcgaacttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     3421 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     3481 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     3541 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     3601 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     3661 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     3721 acccgtatta atgtcacggc ctatcagggc aaagtgctgc tggttgggca gtcaccaaat
     3781 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     3841 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     3901 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     3961 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     4021 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggttac cacagcgttt
     4081 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     4141 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     4201 ccctcaaatc agaacaacag cgccagcccg ccgacaatca caccagacac tgccaccatc
     4261 gcccctgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     4321 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     4381 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     4441 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     4501 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgcaaac cgggatcgta
     4561 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     4621 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcagtgttt cacgcaccca tttttgcacc
     4681 agcgtcgcaa tcagcaacgc catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     4741 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     4801 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     4861 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     4921 cgaaagcgcg attgcccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     4981 aacgaaccga ggatcacccc cagcaccgcc gctttccata ctgcgaggaa atagatcatc
     5041 gcgtaatcca acgccgcttg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     5101 atactgtggg tttcggctgc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     5161 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     5221 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     5281 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     5341 aattctgaag agcttaaaat cgtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     5401 atgacccgcg ccacgtctct cgcatcaatg gatttccagt tacctggtaa caaacggaac
     5461 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag taacatcgac
     5521 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     5581 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     5641 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     5701 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     5761 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     5821 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcagcaat ggcgttaact
     5881 ttcggttcat taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     5941 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccccggctc
     6001 atgcaccagt cttaatagac catgaggtaa ttatccccga ttgtggaaaa ttcgcctcat
     6061 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cactgatgaa
     6121 tttgaagatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     6181 attactattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     6241 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gttgctaccg
     6301 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     6361 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct actgatcagc
     6421 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     6481 aatgcgggcg tggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     6541 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     6601 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     6661 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     6721 attgtacgcc ttgcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     6781 aaactgcaca ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     6841 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     6901 ggcgactttc tgcgcatcaa aaagataaaa ggcattagcg caccagagtt ccccttcctg
     6961 ctgcacacac caggtgacaa catgttgttt tgccagccaa cggctgatgg cgatgagtgt
     7021 ttccattgct gttctctttt atactgtggg cctgacctta acatgcgatg cagacgatga
     7081 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     7141 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     7201 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgaccgttcg ctggcactac
     7261 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     7321 gcgcagggtt tgcagaactg ttgagcagcc tgcaaacccc ggagattaaa agcgattgaa
     7381 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     7441 taccaggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     7501 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     7561 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     7621 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     7681 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     7741 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     7801 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     7861 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     7921 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     7981 tcaatggcgt ccatcaggtt tttcacatac acccccagct ccgtatcgcc ttcaatcacc
     8041 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     8101 tcgctggcgt cggcactaaa actcacatca gcttgcgtgt tctggctgac caccagtttg
     8161 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     8221 cggccttcaa gaaactccag ctcgccatca tccagcgcct gacggaattg ccagcttaag
     8281 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     8341 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     8401 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     8461 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     8521 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gatagcgcaa
     8581 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
     8641 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac caatgcccgt
     8701 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
     8761 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
     8821 cgttggcagc gtgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
     8881 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgaacg tcatgtgtca
     8941 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgacgtt
     9001 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtt
     9061 actcctgtcc cgctggaagt gtttgctttc ggcagcctgt gcattatgtc ggaaggccgt
     9121 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ctgtgggtgc gtgttctccg
     9181 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
     9241 ctgatcgacc gttatcagga cggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
     9301 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
     9361 gaactgctgc cggagttaat ggctgcgaat attgcttcgg tgaaaattga aggccgccag
     9421 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
     9481 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
     9541 tccgaaggca cgcaaacctc ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
     9601 tgaaatattc cttagggcca gtgctgtggt actggccaaa agagacgctg gaagattttt
     9661 atcagcaagc cgccgccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
     9721 agcgccgggc aaccaaagtt ggcgactgtc tggagatggc aaaatcgctc gccggaagtg
     9781 gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
     9841 tgaaacgcta tgttgagaac ggtgaatttc tgattgaagc cagcgatctc ggcgtggtga
     9901 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
     9961 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    10021 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagctgggc attcgcaacc
    10081 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    10141 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    10201 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    10261 agaccatgag cggctacgtt tacaacctcg gtaacgagct ggcatccatg cagggattgg
    10321 ttgatgtagt ccgcctgtca ccgcagggca ctgacacttt cgcgatgctc gacgccttcc
    10381 gcgctaatga aaatggcgct gtgccactgc cgttgacggc aaacagcgac tgtaacggct
    10441 actggcggcg gctggcagga ctggagctgc aagcctaagt aaatagctca ctttgttaac
    10501 aactttaact attctttaat gcagtattaa agattaatcg gtaacaaagt gagctgttat
    10561 gactgataaa accattgcgt tttcgctact cgatctggcc cccatacccg aaggctcttc
    10621 agcgcgggaa gcgttctccc actctctcga tctcgcccgt ctggctgaaa agcgcggcta
    10681 tcatcgctac tggctggcag aacaccacaa tatgaccggc attgccagtg ctgcaacatc
    10741 ggtattgatt ggctatctgg cggcgaatac caccacgctg catctggggt ctggcggcgt
    10801 gatgttgcct aaccacttac cgttggtcat tgccgaacag ttcggcacac tcaatacgct
    10861 ctatccgggg cgaatcgatt tggggctggg tcgtgctccg ggtagtgacc aacggacaat
    10921 gatggcgcta cgtcgtcata tgagcggcga tattgataat ttcccccgcg atgtcgcgga
    10981 gctggtggac tggtttgacg cccgcgatcc caatccgcat gtgcgcccgg taccaggcta
    11041 tggcgagaaa atccccgtgt ggttgttagg ctccagcctt tacagtgcac aactggcggc
    11101 gcagcttggt ctgccatttg cgtttgcctc acacttcgcg ccggatatgt tgttccaggc
    11161 gctgcatctt tatcgcagta acttcaaacc gtcggctcgg ctggaaaaac catacgcgat
    11221 ggtgtgcatc aatattatcg ccgccgacag caaccgcgac gctgaatttc tgtttacctc
    11281 aatgcagcaa gcctttgtga agctgcgccg tggcgaaacc gggcaactgc cgccgccgat
    11341 tcaaaatatg gatcagttct ggtcaccgtc tgagcagtat gacgtgcagc aggcgctgag
    11401 tatgtcgttg gtaggcgata aagcgaaagt gcgtcatggt ttgcagtcga tcctgcgcga
    11461 aaccgacgct gatgagatta tggttaacgg gcagattttc gaccaccagg cgcgcctgca
    11521 ttcgtttgag ctggcgatgg atgttaagga agagttgttg ggatagtgtg tcttaacgcg
    11581 ggaagcctta tccgagctgg caacgctgtc ctacgtaggc ctgataagcg aagcgcatca
    11641 ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc tgattactga tacaccggca
    11701 gtaaattaaa gctcgataaa atatgcacca gtgcgttgcc gacgccaaac accagaatca
    11761 gcgcaatcat cggcttgcca ccccagacgc ggaatttcgg gctgccaaag cgtttacgcg
    11821 atgcgcgggc cagcagtgcc ggaacaattg ccgcccagat ggtagccgct aaaccagcat
    11881 aaccaatggc atacaggaat ccgttcggga acagcagacc acccacaaca ggcggggcaa
    11941 aggtcagcaa tgccgttttc aagcggccca cagccgagtc gtcgaaacca aacagatctg
    12001 ccagatagtc aaacaaaccc agcgttacgc caaggaacga actcgctacc gcaaagtttg
    12061 agaacacgac cagcagaagg tccaggctac ggctgttcag cacgccgctt aatgcttgta
    12121 ccaacacatc aatattaccg cccttctcgg cgatgccgat aaactccgga cgagggatgt
    12181 tgcccattgt cgccagcaac cagatggtat acagcgccag tgccatcagc gtaccgtaca
    12241 ccaggcattt cacgatggtt ttcggatctt tgccgtaata cttcatcagg cttggcacgt
    12301 taccgtgata accaaacgac gccaggcaga acggcagggt catcagcaaa tacggcgcat
    12361 aagatgcatt gctttcggca acgttgaaca atgtcgcagg ctgcacatgc cccagcaggc
    12421 taccgaaggt gaggaagaag gtaatgactt tcgcccccag cacaatcgcc gtcatgcggc
    12481 tgacggcttt agtactcaac cacaccacaa acgctaccag cagtgcaaaa ccaaaacccg
    12541 ccgcccgtgc cgggacgttt agcgacatct ctgcgaaggt gtgatgcaga atcgaaccac
    12601 tggcagaaat ataggcatag gtcaggatat agagcacaaa ggcaatggaa atgccgttga
    12661 ccacgttcca gcctttgccc agcaaatctt tggtgatggt gtcaaaactc gaaccgattc
    12721 ggtaattcag gttagcttcc agaatcatca agccggaatg cagcatacag aaccaggtaa
    12781 agatcagcgc tgccattgac cagaaaaacc acgccccgga catgaccact ggcagagaaa
    12841 acatccctgc gccaataatg gtgccgccga taatcaccac gccgccaagc agcgacggtg
    12901 acgtttgggt ggtggttcgt gttgccatga gggcttctct ccagtgaaaa atagtgcgac
    12961 tgcgttgtta tgcattgcac tgtaccagta catgagtaca aaagacagaa aaaaagcccc
    13021 gatggttaaa atcggggctg tatatattat tttacagatt gtgttcgctg ttcagcgatg
    13081 attacgcatc accaccgaaa cgacgacgac cggtagagtc atcacgacgc ggtgcgcggc
    13141 cttcacgacg ttcgccgcta aaacgacgac catcaccacg accaccttca cggcgttcac
    13201 cgctgaagtt acgaccgcct tcacgacgct cgccaccgaa accacgacca ccgccacgac
    13261 gctcaccgcc agtatgcggc tgtgcatcgc ccagtaactg catgttcatc ggcttgttga
    13321 gaatgcgagt gcgcgtaaag tgttgcagca cttcacccgg catacctttc ggcagttcga
    13381 tggtggagtg agaagcaaac agcttgatgt taccgatgta acggctgctg atgtcgcctt
    13441 cgttagcaat cgcgccaacg atatgacgaa cttcaacacc atcatcgcgg cccacttcaa
    13501 tgcggtacag ctgcatatcg ccaacatcac gacgttcacg acgcggacga tcttcacggt
    13561 cgccacgcgg gccacggtcg ttacgatcgc gcggaccacg gtcatcacgg tcacggaatt
    13621 cacgtttcgg acgcatcggc gcatctggcg gaacaatcag agtacgttca ccctgtgcca
    13681 ttttcagcag tgccgcagcc agagtttcga gatccagctc ttcaccttct gcagtcggct
    13741 gaattttgct cagcagtgcg cggtattgat ccagatcgct gctttccagc tgctgctgta
    13801 ctttagcggc gaatttttcc agacggcgtt tgcccagcag ttctgcgttc ggcagttcta
    13861 cttccggaat agtcagcttc atagtacgtt caatgttgcg cagcagacga cgctcgcggt
    13921 tctcaacgaa cagcagcgcg cggccagcac gacccgcacg accggtacga ccgatacggt
    13981 gaacgtaaga ctcagaatcc atcgggatat cgtagttaac caccaggctg atacgctcaa
    14041 cgtccaggcc acgggccgca acgtcggtcg caatcaggat gtccagacga ccgtctttaa
    14101 ggcgttccag tgtctgctca cgcagcgcct ggttcatgtc accgttcagc gcggcgctat
    14161 tgtagccgtt acgctcaaga gcttcagcca cttccagagt cgcgtttttg gtacgaacga
    14221 agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg taccagtgct tcgtttttac
    14281 gcatacccca cacagtccag tagctctggc tgatgtcagg acgggtagtc acgctggact
    14341 gaatacgcac ttcctgcggc tctttcataa agcggcgggt aatgcgacga atcgcttccg
    14401 gcatggttgc agagaacaga gcggtctgat gaccttccgg gatctgcgcc ataatggttt
    14461 caacgtcttc gatgaagccc atgcgcagca tttcgtcagc ttcatccaga accagaccgc
    14521 tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc cagcagacgg cccggagtac
    14581 cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg cacgtcataa cgctggccgc
    14641 cgtacagggc aaccacattt acgccgcgca tgtgtttaga gaaatccgtc attgcttcag
    14701 caacctgtac cgccagttcg cgggtcggtg ccagcaccag aatctgtggt gctttcagct
    14761 caggatcaag attctgcagc agaggtaaag agaatgctgc agtttttccg ctccccgtct
    14821 gggccatacc cagaacgtcg cggccattca gcagatgtgg aatacactct gcctgaattg
    14881 gagatggttt ttcgtaaccc agatcgttaa gggcttcaag gataggagcc ttcaggccca
    14941 gatctgcaaa agtggtttcg aattcagcca tgtagtacgt gtgcctcaaa attaatggcg
    15001 gccagtctac ataactcatc atgaaattga tcagcaattt tcattgaaaa gtgtgaaccg
    15061 gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc cgttaaggtg atggcaatca
    15121 aaaaagatta cgggctgatg tgtacgtcag ctattgctgg tccgattctg ccaggtcatc
    15181 ttggtcctgg cccaggagcg ataattccaa caatgcgtat cggtgctcaa caaagttatg
    15241 aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg ctgtccaaat cccccagact
    15301 taggtagtac ttacctaaat agaagttggt ttcactgaga tgctcagcga gcgaggtgtt
    15361 atccgttgcg tccgccttga gcctttccat taacgtttgt tcgctaatgt tgcccaggta
    15421 gaactcgaca atgttccatc cccactgttc cttatccgat ttttcgaagt gctgtttcaa
    15481 tacttcttta gcctgcttct catcgagctt ctgctcggcg agataaagcc acagactacg
    15541 gaaaggatca ttgggatcgt cttgataaaa cgccagcaga tcatcttgcg ctaacttgtc
    15601 acgaccgccg taatataatg caatcccgcg attcaagtgc gcgtagttgt aagttggatc
    15661 aagctcaagt acagaatcaa acgcttcata ggcagcatca aaattgcctg cctgcgttaa
    15721 atatatgcct aagtaattga atacttcagg catatccggt cggattgcca gcgcttgcga
    15781 aaaatcgtta cgcgctaatg ccctcagacc gagactatca tacaacactc cgcgctcata
    15841 taaaagctgt gcgcgttcgt catcggttaa agcccgactg gcaaggattt gttccatacg
    15901 tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt accgcgagga cttcactttt
    15961 acgccaggaa gtattactgc atcctgcaag cgtaagtgct gtcgcaacga aacaccagcg
    16021 caaaaaaggc ttcatttccc actcccgaag accacggttg aatgaacgtc ctgttcccgg
    16081 ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc gcagcggagg gcaaatggca
    16141 accttactcg ccctgttcag cagccggagc ttccggtgct gcagcaggtt gagactgctc
    16201 agtcgcttct ttaatgctca gacggatacg gccctggcga tcaacttcca gaactttcac
    16261 cggtacttcc tgacccatct gcaggtaatc ggtcactttc tcaacgcgtt tgtcagcgat
    16321 ttgagagatg tgtaccagac cttctttacc gccgccgatg gcaacaaatg cgccaaagtc
    16381 aacgatacgg gtcactttac cagtgtagac gcggcccact tcgatttctg cagtgatctc
    16441 ttcgatacga cgaatagcat gtttcgcttt ctcgccgtcg gtcgctgcaa tcttcacagt
    16501 accgtcatct tcgatttcga tggtggtgcc agtttcttcg gtcagggcac ggataacaga
    16561 gccgccttta ccgataacgt ctttgatctt gtccgggttg atcttgatgg tatggatacg
    16621 tggtgcgaac tcagagatat cgccacgcgg cgcgttgatc gcctgttcca ttacgcccag
    16681 gatatgcaga cgcgcacctt tagcctggtt cagcgcaacc tgcatgatct ctttggtgat
    16741 accttcaatt ttgatatcca tctgcaatgc agagataccc tcgcgggaac ccgcaacttt
    16801 gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg tcagacagta caacgtagtt
    16861 gtcgccttct ttcaccagac ccattgcgat acccgcaacg gcagctttga tcggcacacc
    16921 tgcgtccatc agcgccagag acgcgccgca cacggaagcc atagaagagg aaccgttgga
    16981 ttcagtgatt tcagacacaa cacgtacggt gtacgggaat ttgtccatat ccggcatgac
    17041 tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt tcacgacgct tcggagaacc
    17101 gaccatgccg gtttcgccta cggagtacgg agggaagttg tagtggaaca ggaaggtatc
    17161 ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca cgagcagtac ccagcgttgc
    17221 ggtaaccagc gcctgcgttt caccacgggt gaacagcgca gaaccgtgag tacgcggcag
    17281 cacgccagta cgcacatcca gaccacggat catatctttt tcacgaccgt cgatacgcgg
    17341 ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg atcgcgtgca gaatttcacc
    17401 cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc gtcgcgatgg tttcagattt
    17461 gatgacatca acctgcgcat aacgctcttg tttgtcggtg atgcggtaag catcgctcag
    17521 gcgagcttca gccagtgcag caacgcgcgc gttcagcgct tcgtttaccg gctccggctg
    17581 ccagtcccaa cgtggtttac cggcttcttt caccagttca ttgatgttct gaataacaac
    17641 ctgctgttgt tcatgaccga acactactgc gcccagcatc tggtcttcgc tcagcagttc
    17701 agcttcagat tcaaccatca gtacagcggc ttcagtaccg gcaacaacca gatccagttt
    17761 gctctctttc aactcgtcct gagtcgggtt cagtacgtac tggtcattga tgtaacctac
    17821 gcgagcagca ccaatcggac cattgaacgg aataccagac agagacagtg ctgcggaagc
    17881 accaatcatc gcgacgatat ccgggttaac ttgcgggtta acagaaacca cggtggcgat
    17941 aacctgaact tcgttgacga agccttccgg gaacagcggc cgaatcgggc ggtcaatcag
    18001 acgcgcgatc agggtttcgc cttcgcttgg gcggccttca cgacggaaga agctacccgg
    18061 gatacgacca gcagcgtagg tacgctcctg atagttaacg gtcagtggga agaagtcctg
    18121 acctggtttg gcttttttct ggccaacaac ggtaacaaat accgcggtgt catccatgct
    18181 aaccataaca gcggcagtag cctgacgagc catcatgccg gtttccagag tcacggtgtg
    18241 ttggccgtac tggaatttac gaacgatcgg attaagcaat gtaatatcct ttctctttct
    18301 tagacagtac cttacggcac tggtgttaat acccgatctt ctgcgcatcc tcgcgactaa
    18361 tgacaaccct aacccagctc tatgtgggta aagcctctca ttagccgcgc gaacctctgc
    18421 aacggaagat cattcatagc aacaatacat tagtttccag tgaattgctg ccgtcagctt
    18481 gaaaaaaggg gccactcagg cccccttttc tgaaactcgc aagaattagc gacgcagacc
    18541 caggcgctcg atgagctggg tgtaacgtgc tacgtcttta cgtttcaggt agtcgagcag
    18601 tttacgacgc tgagaaacca tgcgcagcag accacgacgg ctgtggtgat cttttttgtg
    18661 ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc agcagtgcta cctgaacttc
    18721 agtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa acgattttag ctgttgcttc
    18781 agtacttaga gacattttaa aactccaaag tatatagaat gaaaggacgc cgatctctaa
    18841 ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa tttacgcgac gttaagcggc
    18901 agtattctac tcgtagcgac ctgttatcgc aagacggtta acattacgcc gggtattcaa
    18961 ccaccaggcg acgaggcgca acgcggcctt catcgtcaat ttcgcccata ccgataaatt
    19021 tgccgttctc accttctgta acgcgaacca gtccttccag cggcgcaccg gatgtacgaa
    19081 ctgggttacc atttttgaag taaacagaag acgttaacgg aagattcacc accgggtagt
    19141 ccgaagctgg actgtccatt ggcatcagta atggatcaag taactccgca gccggaatat
    19201 cctgctgttc ggcttgttcg accagttcac gcagatgttc cagggtcaca atccgttcaa
    19261 ccggatattt acttaccgcc agacgacgca ggtaaataac atgcgcgcca caaccgagtt
    19321 tttcacccag gtcatcaatg atggtgcgga tataagtgcc ttttgagcag tgaatttcca
    19381 gctccagctc attgccttcg tggcgaataa acagcaattc ataaacggta atcgggcgcg
    19441 cttcacgcgg tacttcaatg ccctgacgcg catattcgta cagtttttta ccctgatatt
    19501 tcagtgctga atacatcgaa gggatctgtt cgatatcgcc acggaaagta tccagcgccg
    19561 ccgccagttg ctctgcgcta aaggttaccg gacgttcttc aacgatttgt ccgtcggcat
    19621 cagaagtatc ggtacgctgt ccaagacgcg caatgacccg atagcgtttg tcggagtcca
    19681 gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat tggcaacatg ccagttgcca
    19741 gcgggtccag cgcaccggta tgcccggcac ggttggcgtt ataaatccgt ttcacttttt
    19801 gcagcgcatc attgctggac ataccctgcg gtttatccag caacaaaacg ccgttaatgt
    19861 cgcgaccgcg acgacgagga cgactcatta gtcctccttg ctgtcgtccg ggttaacacg
    19921 acgttcttcg tcatgtttga ccacgctggt caccaggttt gacatgcgca tcccttcaac
    19981 cagagagttg tcgtagaaga aggtcagttc cggcacgata cgcaggcgca tcgctttccc
    20041 cagcaggctg cggatgaaac cagaagcttc ttgcaacgct ttgatgcccg ctttaaccgc
    20101 gtcttcatct ttgtcgttga ggaacgtcac atatactttg gcatacgcca ggtcgcgaga
    20161 catttcgaca ccggaaacgg tggtcatcat gcccaggcga ggatctttaa tttcacgctg
    20221 caggatgaga gcgatctctt tttgcatttc ctgcgctacg cgctgcgggc gaccaaattc
    20281 tttcgccata ataaattctc ctgacaaaaa aggggctgtt agcccctttt taaaattaat
    20341 ttcaggtgga agggctgttc acgttggcct gataagacgc gccagcgtca catcaggcaa
    20401 tccatgccgg atgcggcgta aacgccttat cccgcatgga accctaaaaa ccttaagcga
    20461 tggtacgttg gatctcgatg atttcgaata cttcgatcac atcgccagtg cggacgtcgt
    20521 tgtagttctt aacgccgata ccacattcca taccgttacg gacttcgtta acgtcatctt
    20581 tgaagcggcg cagggactcc agctcgcctt cgtagataac cacgttgtca cgcagaacgc
    20641 ggatcgggtt gtgacgttta accacacctt cggtaaccat acagcctgcg atggcaccaa
    20701 atttcggcga tttgaacacg tcacgaactt ccgccagacc gataatctgc tgtttcagtt
    20761 ccggagacag cataccgctc atcgccgctt tcacttcgtc aatcaggtta tagatgacgg
    20821 agtagtaacg cagatccagg ctttccgctt caatcacttt acgtgcagag gcatcagcac
    20881 gtacgttgaa gccaaccagg atggcgttgg acgccgcagc cagggtggcg tcggtttcgg
    20941 tgataccacc tacgccagaa ccgatgatct tcactttaac ttcgtcagta gacagtttca
    21001 gcaaggagtc ggagatcgct tccacagaac cctgtacgtc tgccttcagg acgatgttca
    21061 cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt ctcgagttta gatttctgct
    21121 gacgcgccag tttaacttcg cggaatttac cctgacgata cagtgcaact tcacgcgctt
    21181 tcttctcgtc acgtacaacg gtaacttcat cacccgcagc cggtacgccg gacaggccga
    21241 ggatttccac cggaatggac ggacccgctt ccagcacttc ctgacccagt tcgttacgca
    21301 tcgcacgaac acgaccgtat tcgaagccac acagaacgat atcgcccttg tgcagagtac
    21361 cttcacgtac cagaacggta gcaaccggac cacgaccttt atcgaggaag gattcgataa
    21421 ccgcaccgct cgccatacct ttacgtaccg ctttcagctc cagaacttcc gcctgcagca
    21481 ggatagcgtc cagcagttca tcgataccag tacccgcttt cgcagacaca tgtacgaact
    21541 ggctttcacc gccccactct tccggcagga tgccgtactg ggagagttcg ttcttaacgc
    21601 gatccggatc ggcttctggt ttatcgatct tgttcactgc aacgaccacc ggaacctgcg
    21661 ccgctttcgc gtgctggatt gcttcgatcg tctgcggcat tacgccgtcg tcggcagcaa
    21721 caaccaggac tacgatgtcc gttgcctgcg caccacgagc acgcattgaa gtaaacgcgg
    21781 cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc agtttcaacg tggtatgcac
    21841 caatgtgctg ggtaatgccg cccgcttcgc cagaggccac tttcgttgaa cgaatgtagt
    21901 ccagcagaga ggttttaccg tggtcaacgt gacccatgat ggtcacaacc ggcgcgcgcg
    21961 gttcagccgc agcacccgtg tcacggtcgc tcattaccgc ctcttccagc tcgttttcac
    22021 gacgcaggat aactttatgg cccatctctt cagcaaccag ctgtgcggtt tcctgatcga
    22081 taacctggtt gatggttgcc attgcgccca gtttcatcat cgctttgatg acctgagagc
    22141 ctttaaccgc catcttgttc gccagttcgc caacggtgat agtttcgccg atcacaacgt
    22201 cacggttaac ggcctgagca ggcttctgga agccttgctg cagcgaagaa cctttacgtt
    22261 ttccgccttt accgccacgt actgctgcgc gtgcttcttc acgatcagct tttgattcag
    22321 cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt acgaccacgg ccacggccgc
    22381 cttcgacttc acgatcgctt tcgtcttctg cctggcgagc atgttgagaa gtagtgacgt
    22441 gataatcgct ggaatcttca gtcggttccg cgttatcagt ccatttgttt tcttccgcca
    22501 tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc gagtttacga cgcgcttctt
    22561 cttcagcttt acgcttgagc tctgcagctt cctgctcacg gcgggctttt tcagcctggg
    22621 cgtttttagt catatcgtct tgttgattgc tcactttgtc tttttccgca gcttcacgtt
    22681 tcgcttgttc agcagcttca cgcttagctt gttctgcggc ctcacgttca gctttttgtt
    22741 gcgcctcgcg tttagccgat tcttctgcct cacgacgggc ttgctcttcc gctgcaaggc
    22801 gttcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact tcgatttgta
    22861 ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt ttacgttgca
    22921 gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa gtctgtttct
    22981 cttgtgcaga cacagagtcg tcagcagact tccggatacc tgcatcagca aattgctgta
    23041 ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt acatctgtca
    23101 tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa atattacggg
    23161 cagccataat cagtgctccg gctttttcgt cggtcaaccc ttcgatatca gccagatcat
    23221 caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc agtttgaatg
    23281 ccaaatcacg atctacccct tcaaggttca gcagatcgtc agccggtttg ttatcaccga
    23341 ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg
    23401 cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc ggcacatagg
    23461 ccagttcttc cagcgtcgag aaaccttctt ctaccagaac agtcgcgaag tcttcgtcga
    23521 tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct
    23581 gcaggtcgtc aacggtcatt acgttgagtt cccaaccgct cagctgcgaa gccagacgca
    23641 cgttctgacc gttacggcca atcgcctgcg ccaggttacc agcttcaacg gcgatatcca
    23701 tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgccggtgcc attgcgttaa
    23761 tcacaaactg cgccgggtta tcatcccaca ggacgatatc gatacgctcg ccgcccagtt
    23821 cagtagaaac cgcctgaaca cgcgcgccac gcatacctac gcaagcacct accggatcga
    23881 tacgtttatc gttggttttc accgcgattt tcgcacgaga acccgggtcg cgagccgctg
    23941 ctttaatttc aatcacttct tcgccgattt ccggcacttc aatacggaac agttcgatca
    24001 gcatttccgg cttggaacga gtgacgaaca gttgcgcgcc acgcgcttcc gggcgaacgg
    24061 aataaagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc agcatatctt
    24121 cgcgcaggat cacggcttca gcgttgttgc ccagatccag agagatgttg tcgcggttta
    24181 cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga tcaaccacca
    24241 tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc tgggtggtga
    24301 tacggtcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccaag ttcaggcttt
    24361 catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg acttcatcaa
    24421 caactaacca gcgacggaag gtgtcaaaat caccgctttt gcgatcgatc tgtacacgga
    24481 cgtcgatctc ttgctcatat tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt
    24541 cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact acagccaaaa
    24601 tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg ggaaccaggt
    24661 tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg gtaactgtga
    24721 tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt tgtaccgcca
    24781 tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca gccgtgaaca
    24841 gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg atgggatctt
    24901 caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca acattgatgc
    24961 catcttcact atcaatatag atgcgcagtg tggatgtgcg accgcgaata aattcgatgc
    25021 ctaccagttc aaaacccagg gcctcaactg gcgcagtaat catctctgtt aatttttgct
    25081 ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc agttattctg
    25141 tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac cctataaccg
    25201 caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac gaaggccgaa
    25261 gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg gccggatttg
    25321 aaccgacgac cttcgggtta tgagcccgac gagctaccaa gctgctccac cccgcgcctg
    25381 aaacgtggca aattctactc gttttgggta aaaaatgcaa atactgctgg gatttggtgt
    25441 accgagacgg gacgtaaaat ctgcaggcat tatagtgatc catgccacat tttgtcaacg
    25501 tttattgcta atcacgtgaa tgaatatcca gttcactttc atttgttgaa tacttttgcc
    25561 ttcccctgct ctcccttaag cgcattattt tacaaaaaac acactaaact cttcctgtct
    25621 ccgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca gttagagcgg
    25681 aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga cgattctcaa
    25741 gcatctcccg gtaggtcaac gtattggtat cgctttttcc ggcggtctgg acaccagtgc
    25801 cgcactgctg tggatgcgac aaaagggagc ggttccttat gcatatactg caaacctggg
    25861 ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat acggcgcgga
    25921 gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg ccgctattca
    25981 gtgtggcgca tttcataaca ccaccggcgg cctgacctat ttcaacacga cgccgctggg
    26041 ccgcgccgtg actggtacca tgctggttgc tgcgatgaaa gaagatggcg tgaatatctg
    26101 gggtgacggt agcacctata aaggaaacga tatcgaacgt ttctatcgtt atggtctgct
    26161 gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta ttgatgaact
    26221 gggcggccgt catgagatgt ctgaatttat gattgcctgc ggtttcgact acaaaatgtc
    26281 tgtcgaaaaa gcctactcca cagactccaa catgcttggt gcaacgcatg aagcgaagga
    26341 tctggaatac ctcaactcca gcgtcaaaat cgtcaacccg attatgggcg tgaaattctg
    26401 ggatgagagc gtgaagatcc cggcagaaga agtcacagta cgctttgagc aaggtcatcc
    26461 ggtggcgctg aacggtaaaa cctttagcga cgacgtagaa atgatgctgg aagctaaccg
    26521 catcggcggt cgtcacggcc tgggcatgag cgaccagatt gagaaccgta tcatcgaagc
    26581 gaaaagccgt ggtatttacg aagctccggg gatggcactg ctgcacattg cgtatgaacg
    26641 tctgttgacc ggtattcaca acgaagacac cattgagcag tatcacgcgc atggtcgtca
    26701 gttgggccgt ctgctgtacc aggggcgttg gtttgactcc caggcgctga tgctgcgtga
    26761 ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttaccctgg agctgcgccg
    26821 tgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca agccagagcg
    26881 tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtattg gtcaattgac
    26941 catgcgtaat ctggatatca ctgatacccg agagaaactt tttggttatg ccaaaactgg
    27001 cctgctttcc tcctctgcca cttcaggcct gccgcaggtg gagaatatgg aaaacaaagg
    27061 ccagtaattc gacatcaacc ctgcccttca ggggtggggt taccttgctt atcctctgta
    27121 aactcctttc tgagcatcac ataatcgaaa acatcatggt ttgcatccac aacccgtgag
    27181 tctcctttat agtgtgcaat cacttcctca agcgtttgcg gctgttccgg ctttgttacc
    27241 cccatccacg cattaataag gtaattattg taagctgtcg aaaagatatt gttttccgtt
    27301 gtgcgatcca cgccatcccc aagaacgggg ctataccaga taaacatcgg gacatgatat
    27361 gcctgctggc tagcctccct gcctccatga aaatagacgt tcttcttcgt agggtcacgt
    27421 tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa taattcaaaa
    27481 acctgaccta gcaaactatc tgtgtaatga atggagttgt catagcaggc atcctgatcg
    27541 tcctgcggtt gaaacacggc gctggattgc ggataggcgc tacaagccgg ttcatggctt
    27601 ccatttaaat gaagaacaat cagtttcttt tgctgcgtat tttgctgtaa tgcttgcgat
    27661 aaatgcggca acaacaattc atcaaatcct ctgacataaa ctgtttccat ggcgcgcatg
    27721 gcgatactgg taactgctgt accattctgc cgaaaagcgg attgcgagct tagccagaaa
    27781 gtctgaaatc ctgcttgatt agccatatta ataatgttgt cggggtaatt atgaatgtca
    27841 tgactcaaaa cagaatcagc agttaaagaa aggggaaccg acagcgcagt gtaaggtgcg
    27901 ccgcttattg cttgattaaa cagtttgatc tgttttcttt gtgcttcaac ttgcggtgtc
    27961 gtagagcgtg tatatccata caaagacata ttgtcgacac gtacagactc ccccacaatc
    28021 aacacgtagg tatcaatact tgtatccctg actgataatt gaaaatacgg caccgtgttt
    28081 gcaattgaga gtaatctttg atgctctttc gctgctaaag caaaatagtt gagattgaaa
    28141 aacggcgtat aggtagcaaa tcacgacgct agtatatatg gactgaacgc ttttttattt
    28201 tttgcatctt tataagcaaa ttgacaagcg gaaaataaac tgcccgaaat gacaatcagc
    28261 aataatattc ctgtcacttt ttttgtcggc aaggaaacat catattttat tattactgcc
    28321 aaaaaaagaa gggataaaaa agcaaaagca catagataag ggatatacat ccccagcatt
    28381 ttcacaactt catccggatc actctgcagc acactaatcg caaatccatc attgaaagtt
    28441 gtaccaaagg tacaccatga atagaggcta atactcatgt ctgccgcagt cagtaacaca
    28501 aatggtgcgg caacaagcag gcgaagcgga agggatgata tacgtttaac aaccaggatg
    28561 gtcaggacga agaacagcaa tcgctgaacc atatgcgctc cggaagaggc gactaagttc
    28621 gtaataccaa aaacaataac acaaagatac aacaaccaat gagatttaaa atcttctagc
    28681 aaatttgttg aatactgtca tccagaaatc caagttcaca accaatataa acaattatca
    28741 acaatgttca attttatatc agacatgttt ttcaactctg atccatatca gaccgaaaca
    28801 ctgttagttt tttcttttta aatgatgcga ccattgcgtt ctcagtaatt aaataacatt
    28861 atcaatgcgt ctctaaacat tacgcagaac gtagccatca gtagttatac tgacaggagc
    28921 aggatggttt atcgagaagt aaacatatcc gtattcgtac cggaactgga gttgacacaa
    28981 taaagtgcca attatgtcag cagtagggaa aagtttataa ctaaggcgta ccggcaccat
    29041 cgtttcaagg taccagctac gagtaaagca actggacgag atacagatac ctgacaacca
    29101 ttcatcagac caggaccaaa acgaaaagag acgcttttca gcgtctcttt tctggaattt
    29161 ggtaccgagg acgggacttg aacccgtaag ccctattggg cactaccacc tcaaggtagc
    29221 gtgtctacca attccaccac ctcggcacgg atactacttt ttagttcggg atatcgctgg
    29281 tcggcttagc cggagcagct ggctgagttt gttcggtttt cgccggtgca ctcagatttt
    29341 cccattcgct acctctattg gttttgttgc tattgatgtt acccagcacc agactgatga
    29401 tgaagaataa cgttgccagc agcgccgtca tgcgggtcat gaagttacca gaaccacttg
    29461 aaccaaacag cgtagcggaa gcgcctgctc cgaaggaggc tcccatatca gcgcctttac
    29521 cttgctgcag catgatcaga ccaacaaggc caattgccac aataaggaaa actactaaaa
    29581 gagcttcgta cataatcaac ctgttccttg cggagttgcc gcgtaccaat gcttcaacca
    29641 ataaagcggg agtttttaat ctttcccact gaagcgggtg tgaatactaa ccaaaacgaa
    29701 tgaccttcgc aagggcaatt ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc
    29761 gatgtgttcg ccgagaaaaa aggcgacaat cgccgccttt ttagccagtt atctaacgct
    29821 ttaaacggct tttactgcat cggcgatgcg gtgtgcaaat tcagtcacct gcgcttcgtc
    29881 ttcgccttcc accatcacgc gaattaacgg ttcggtgccg gatttacgca gcaacacgcg
    29941 tccacggttg cccagcgcag cttcaacctc tgcggtcacg gctttaactg actcatgctc
    30001 aagcggatcg ccgctacctg cggtgtaacg tacgttaacc agaatctgcg ggaacatttt
    30061 catgccgctg caaaggtcgt gcaggctcat atggttacgt gccatcgccg ccagcacctg
    30121 caagccagca acgatgccgt caccggtagt ggttttatcc agcaggatca catgaccgga
    30181 attctctgca ccgatacgcc agcctttctc ctgcattttt tccagtacgt agcggtcacc
    30241 cactttcgcg cgcgcaaatg gaattcctaa ctgtttcagc gccagttcaa gccccatgtt
    30301 gctcatcaat gtacccacag caccaccacg cagctggccc tgacgaagac cttcacgcgc
    30361 gatgatatac atgatctgat cgccatcgac tttattgcct tcatggtcaa ccataatcac
    30421 gcgatcgccg tcgccgtcga aggcaatacc gagatccgct ttttcagcca gcacacgagc
    30481 ctggagcgcg cgaacgtcgg tagcccccac ttcggcattg atgtttacac cgtttggctc
    30541 acaaccgata gcgataacgt tcgcccccag ttcgcgcagc acgttcggcg cgatgtgata
    30601 agtcgcaccg tttgcacaat ccaccacaat cttcagttca ctgaggctaa gttcgttcgg
    30661 gaacgtggct ttgcaaaact cgatatagcg acccgcggca tcaacgatac ggctggcttt
    30721 acccagttct gccgaatcaa cgcagctgat ctccttttcc atttccgctt cgatggcctc
    30781 ttctaccgca tccggcagtt tagtgccgtc gatagagaag aatttaatcc cattatcgta
    30841 gaacgggtta tgcgatgcag atatcacaat tccggcctct gcgcggaagg tacgcgtcag
    30901 ataagccacg gccggtgttg gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc
    30961 cagacccgct tccagtgctg actccagcat atagccagaa atacgcgtgt ctttaccaat
    31021 aataatctta cgggagccgt ggcgcgccag cactttaccc gcggcccaac ccagcttaag
    31081 cacaaaatca ggtgtgatcg gcgcatcccc tacacgacca cgaatcccat cggtaccgaa
    31141 atatttacga ttactcatag cgtttgtttt cctttgcaga cagagtggct tccaccaccc
    31201 gcatcgcttc tacggtttct ttgacgtcat gaacacgaat gatgtgcgcg ccttgcattg
    31261 cggcaatgac cgcacaggcc agactaccgc tcaggcgctc ggacggcccc acgttcagca
    31321 gctgcccaat catcgatttt cgtgacatac ccaccaacag cggcaggttg aaatggtgaa
    31381 attcagccag gcgcgccagt aatgaatagt tatgggagag atttttaccg aaaccgaatc
    31441 cggggtcgag caacaatttc tcttttgcga tacccgcctg ctcgcaacgt gctatttgct
    31501 caataaagta gcgattcact tctgcaaaga catcgtcata cttcggagct tcctgcatgg
    31561 tttttggatt tccctgcata tgcatcagac aaaccggtaa accggtttct gcagccgcct
    31621 ccagagcgcc aggttcggaa agggagcgga tatcattaat aatgtgagcg ccaactttcg
    31681 ctgactcacg gatgacttct ggtttggatg tatcgaccga gatccagact tcgaagcgtt
    31741 gagcaattgc ctcaaccaca ggaataacac gttgcaactc ttcttcaacg ctaacttccg
    31801 ccgcccctgg gcgcgtggac tcgccaccaa cgtcaatgat cgtcgcgcca gcattgatca
    31861 tcagattcgc atgtttcacc gcatctatca gcgagttatg cgtgccacca tccgaaaagg
    31921 aatcaggcgt gacgttgagg atccccatta cgtgaggatg gctaaggtcc agtgaagtac
    31981 cctgggcaaa gagtttcatt atgttatccc tggtatgaat tgataagaaa aaaccccgga
    32041 gcacgccccg gggttttcgg tacaaataca gtcatctgat gcgggaactt acttgtcgcc
    32101 taactgctct gacatggtgt tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg
    32161 agcctttgga ctaccattgt cgccagaatt gttagaagcg cctggttctt cccagcccgc
    32221 tggcggacgt acatcgcgac gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc
    32281 atatttcatg agagcatctt tcatcgcatg cagaatatcc atattgtcgg tcagaagctg
    32341 acgcgcacga ttatagttac gctcaatcag tgctttcact tcctggtcga tgatacgtgc
    32401 agtttcatcg gacatatgtt tcgctttcgc tacgctacgg ccgaggaata cttcaccttc
    32461 ttcttccgcg tacagcagtg gacccaattt ctcagagaag ccccactgag tcaccatgtt
    32521 acgtgccagg ttggtcgcaa ctttaatatc gttggacgca ccggtagaaa catgttccgg
    32581 cccgtagatg atctcttctg ccagacgacc accgtacagc gtagaaatct ggctttccag
    32641 tttctgacgg ctggcgctga ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc
    32701 acgaccgcgt gggataatcg tcactttatg caccggatcg tgttccggca ccaggcgacc
    32761 gataatcgca tgacccgctt cgtggtaagc cgttgattct ttctgcgctt ccgtcatcac
    32821 catggagcga cgttccgcac ccatcatgat tttgtctttc gctttctcga actcaaccat
    32881 cgacacaacg cgtttgttgc cacgagcagc gaacagtgcc gcttcgttca ccaggttcgc
    32941 caggtcagca ccggagaaac caggagtacc acgggcaatg attgccgcgt cgatatcggg
    33001 tgccaatggt acgcgacgca tgtgaacttt caggatctgc tcacgaccgc gaacatctgg
    33061 caagccgacc acaacctggc ggtcgaaacg gccaggacgc agcagggccg ggtcgagaac
    33121 gtccggacgg ttagtcgcgg cgataacgat gataccttcg ttaccttcga agccatccat
    33181 ctcaaccagc atctggttca gagtctgttc acgttcatcg tgaccaccgc ccagaccagc
    33241 gccacgctgg cggcctacgg cgtcgatttc atcgataaag atgatgcacg gtgccgcttt
    33301 cttcgcctgt tcgaacatgt cacgaacacg ggatgcaccc acaccgacga acatttctac
    33361 gaagtcagaa ccggagatag taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc
    33421 cagcaacgtt ttaccggtac ccggaggacc gaccatcagg acgcctttcg ggatcttacc
    33481 gccgagtttc tggaagcggc tcggctcgcg cagatactct accagttcag caacttcttc
    33541 ttttgcttcg tcgcagcccg caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag
    33601 catgcgcgct ttgctcttac caaacgacat ggcacctttg ccaccgccgc cctgcatttg
    33661 acgcatgaag aagatccaga caccaatcag caacagcatc gggaaccagg agatgaagat
    33721 agaagccagc aggcttggtt cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa
    33781 caggttatcc agtaatttcg gatcctgaac cggaatgtaa gtggtataac ggttactatc
    33841 tttcttggta acgttgattt cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt
    33901 gacctcttgt aggaaggtag agtaatccac cttacggcca ttagactcgc tgggcccaaa
    33961 gctctggaat actgacatca gcacaacggc aatgaccagc cagagtatta ggtttttcgc
    34021 catgtcactc aagggattaa cctcttatta caactgtgtt aaaaacagcg tcaggatact
    34081 ctatatccag catctttcaa actttcgtct gaaatctccc ggttagggtt tacgcccggt
    34141 cgctacaata tacacttctc gcgaacgtgc acgagaagag tccggcttac gaactttgac
    34201 cttcgtaaac agggagcgaa tttcccttag atactcatcg aaaccttcgc cctggaacac
    34261 cttcactaca aaactgccac ctggcgctaa tacatcacga cacatttcta gcgccagttc
    34321 caccagatac atggctcggg ggatatccac cgccggtgtt ccgctcatgt ttggtgccat
    34381 atcggacatg acaacctgga ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac
    34441 cagttcatca cgaaaatcgc cctgaagaaa gtccacacca acgataggat ccataggtaa
    34501 aagatcgcaa gcgatgatgc ggcctttgcc gccaatttgg gtgaccacat attgtgacca
    34561 accacccgga gcagcaccaa ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact
    34621 ttgctgtatt tcatcaagtt taaaccaggc acgggaacgt aatccctttt tctgtgcctg
    34681 ttgaacatat ttatcgctaa agtgttcctg aagccagcgg ctggagctgg cagaacgctt
    34741 cttacctgtc atttaacttt cccatgggga taactcatcg taaccaattg cgtaaatttt
    34801 tacgcgccta tttggtgata tatgggagat ggcggtagaa tgacccgttt tcaatcccaa
    34861 cgtaagcaaa aaatacgatg aatctgagta ctaaacaaaa acagcacctg aaaggtctgg
    34921 cacatccgct caagccagtt gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg
    34981 ccgagattga acaagcgtta gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag
    35041 atcgcgaaac aaaaaccttg atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg
    35101 tacaggtcat cggtaaaacg ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc
    35161 tgccacgcta agattatcct aaagttacac acatttgctg tgtaaaacga ggggttttcc
    35221 gcaggcagga gagcaaaatg ccacgctctg ttcgttgata aaaggccgca gagcggcctt
    35281 tttcctttct ttacaataca tcaacatctt gagtattggg taattcttac aggtattcca
    35341 ccttaattac ttcaaattct acttcgccgc ccggcgtttt gatgaccaca acatcatctt
    35401 cttctttgcc gatcaggcca cgagcaatag gcgagtttac agaaatcagg ttttgtttaa
    35461 agtcagcttc gtcatcgcca acgatgcgat aagtctgttc ttcgtcagaa tccagattca
    35521 gcaccgttac ggtagcacca aaaataacgc gcccattgtt gggcattttg gtgacatcaa
    35581 tcacctgcgc gtttgacagc ttggcttcga tgtctttgat acggccttcg cagaagccct
    35641 gctgttcacg agctgcgtgg tattcggcgt tttctttcag gtcgccatgc tcacgcgctt
    35701 ccgcgatagc agcaatgatt tcaggacggc gcacagattt cagaaaatcc agctcttcgc
    35761 gtaatttttc agcgccgcgt aaggtcatcg gaatagcttg catttgttat acctcttgaa
    35821 tattcctgat ggggcaagtc ttcacccacc ccggctgttc ggcctgcccg gcataactcc
    35881 tcaccgggac cagaagcaaa aaaataccga cccgggtaca agtcccaggt cagctacaat
    35941 tcacattttg atggtcattt taccctgaag ttcccgaagg gtcatcgttt actttatagg
    36001 gcgttgcgcc gtagtatgac ggctcgattc caggttgtta gcgcgagatt atgcgatttt
    36061 ccagatttat catcggattg accagctgta tagcgttcag tgttcaggcc gcgaatgttg
    36121 atgagtacgt tactcaactc cccgcaggtg ccaaccttgc cctgatggtg caaaaagtcg
    36181 gtgcgtcggc ccccgctatt gattaccaca gtcagcagat ggcgctgccc gccagtaccc
    36241 agaaagtgat tactgcgctg gcggcgttga ttcaactcgg ccccgatttt cgtttcacca
    36301 cgacgcttga aaccaaaggc aatgtggaaa acggcgtact taagggtgac ttagtggcgc
    36361 gatttggtgc cgatccgacg ttaaaacgtc aggatattcg caatatggtc gcgactttga
    36421 aaaaatctgg cgtcaaccaa atcgatggca atgtgttgat agatacctcc attttcgcca
    36481 gccacgataa agcccccggc tggccatgga atgacatgac acaatgcttt agcgctccgc
    36541 ctgctgccgc catagttgac cgcaactgtt tctccgtctc gctctacagt gccccaaagc
    36601 ctggtgatat ggcttttata cgcgtggcat cttattaccc cgttacgatg ttcagccagg
    36661 tacgcaccct cccccgtggt tctgccgaag cgcaatactg cgaactggat gtggtgccaa
    36721 gcgacctgaa ccgctttacg ctgacgggat gcctgccaca acgttctgag ccgctcccgt
    36781 tggcttttgc cgtgcaggat ggagccagct atgccggtgc aattctgaaa gatgagttaa
    36841 aacaggcggg tatcacctgg agcggaacac tgctgcgcca gactcaggtt aacgaacctg
    36901 gaacggtagt tgccagtaaa cagtcggccc cgctgcacga tctgcttaag attatgctga
    36961 aaaagtcgga caacatgatc gccgatacgg ttttccgcat gatcggccat gcgcgcttca
    37021 atgtgcctgg aacatggcgg gctgggtcgg acgccgtgcg tcagatcctg cgccagcaag
    37081 ccggtgtcga tattggtaac accattattg ccgatggttc agggctttcg cggcataacc
    37141 tgattgcccc cgccaccatg atgcaggtgc tgcaatacat tgcccaacac gacaatgaac
    37201 ttaactttat ctccatgctg ccgcttgcgg gctatgacgg ctctttgcag taccgtgcag
    37261 gtctgcatca ggcgggcgtg gatggcaaag tctcagcgaa aaccggttcg ttgcaggggg
    37321 tatataacct ggcgggattc attaccacag cgagcgggca acgaatggca tttgtacaat
    37381 atctttctgg ctatgcagta aaacccgcgg atcagcgtaa tcgccgtatt ccgttagtgc
    37441 gttttgaaag ccgtttgtac aaagatattt atcagaacaa ttagtcaaaa agaaaccccg
    37501 gcgcatggcc ggggtttcag attattgaca aagtgcgctt tgtttatgcc ggatgcggcg
    37561 tgaacgcctt atccggccta caaaatcgtg caaattcaat atattgcaat catcttgtag
    37621 gcctgataag tgtagcgcat caggcttatt tggcattggt cattagtgat taacgcttgt
    37681 aaatgaactc aacgccttct tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat
    37741 cctcttcagc aatctcttca agctgctgac ggtgataatc atcccacatg aattcgactt
    37801 tctctggctg tttcgcttct tcagcctgca cgaccgggtt ttcaatgata aaggtcatca
    37861 catcccagca gagatctttc acgcccagtc cgctcgccgc agagatcaga taatatttat
    37921 cttcccagcc cagcgcttca gcgatcactt tcgctttctc ttcggcttct gccttatcca
    37981 gcagatcgat cttgttgaag actaaccaac gcggtttcgc cgccagatcc tggctgtatt
    38041 tttccagctc gctgataata atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat
    38101 cgatatcgat gaggtgcaac aggacgcggc aacgttccag gtgcttcagg aagcgaatgc
    38161 ccagacctgc gccttccgca gcgccttcaa tcagtcctgg aatatcggca acaacgaagc
    38221 tcttttcgtt gtccattcgt accacaccca gacttggcac cagagtggta aacggataat
    38281 ccgccacttt cggtttagcc gccgataccg cacgaataaa ggtcgattta cccgcgtttg
    38341 gcatccccaa cataccgacg tcagccagca gcatcagctc cagcagcagc tcacgcttat
    38401 cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt aactgacgat ttgaaacggg
    38461 tattgcccag accgtgccag ccgcccttag caaccagcag acgctgaccg tgtttagtca
    38521 tatcacccat ggtttcacca gtaccctggt cgattacacg cgtacctacc ggcactttaa
    38581 tcgtcacgtc tttaccgcgc ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct
    38641 ctgcacggaa agatttttca aaacgataat cgataagcgt gttcaggttc tcgtcggctt
    38701 ccatccatac gtcaccacca tcaccgccgt cgccgccatc cgggccgcct ttcggaatat
    38761 acttttcgcg gcggaagctc acgcaaccat taccgccatc acctgcaacg accagaatcg
    38821 atgcttcatc aacaaacttc attttattct ccgtaaatca ttcgcctgcg cggggttgcg
    38881 ataccaccgt tgtatgctta cgtaatccgc cccaaatacg atgaccaatg gcggaataca
    38941 tcgcgcccgc aaccacgaca aacgcaccga gataacctaa aaggtttaac atcggtctgg
    39001 cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa aaacagcgta aacagtgggg
    39061 tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg agccatcgct tccgccaagg
    39121 cgccatatcc taccaaggta ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc
    39181 taagctgcgc tatcactcca ggctttgcca gagggaagag cgcgattgta cataaagtgt
    39241 acagtaaaaa caggatctgc ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc
    39301 cataactcac ccaaaccgtc gccgcaccga ccccaaagat aactccccag gtgtaatcgg
    39361 tgagctttgt aaatatctcg accagactgg tgttaaaaaa catcaccaga ccgctcagga
    39421 gcatcaatgc ccctacaacc tgagtgctgc gcattttctc tttcaggata aatacgctgg
    39481 caaccatcat gccaactggc gagagttgcc caatcacctg cgaagcggtc ggactcaggt
    39541 attgcaagga tgagctgaac aggatgaagt tcccaaacag cccggcggtc gccactgcca
    39601 acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg
    39661 caagaatggc accaaggcca atactcgcca tcaagaaacg gtaaaacacg attgtcggag
    39721 gttccatcac ctccagcacc tgcttcattg cgattggcaa cgccccccag caaattgctg
    39781 tggtaagcgc caaaagaatg ccaatgcctg cctgctgctt cataccgtat tccctgcaaa
    39841 gcgcatttgc ggattaacgt cgcaccatgc gcatttttac cggttatcga atgtaaaaag
    39901 ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact tattcagctt
    39961 cgatgctgat aaatttacgg tttttcgggc ctttaacttc gaatttcact ttaccgtctg
    40021 ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg tggaatttgg
    40081 taccacgttg acgaacgatg atgctacccg ccagaacgga ttcgccaccg aaacgcttaa
    40141 cgcccaagcg tttagcttct gaatcgcgac cgttacgtgt ggagccgcca gcctttttat
    40201 gtgccatttg aaatctctcc tcaggtctta ggcgctgatg ccagtaattt tcacatcagt
    40261 gaaccactga cgatggccct gctgcttacg atagtgttta cgacgacgaa acttaacgat
    40321 tttaactttc tcgccacgac cgtgagcaac aacttcagct ttgattacgc cgccatcaac
    40381 gaaaggaacg ccgattttga cttcttcacc gtttgcgatc atcagcactt cagcgaactc
    40441 aacagtttcg ccagttgcga tgtccagctt ttccaggcga acggtctgac cttcgcttac
    40501 tcggtgttgt ttaccaccac tttggaaaac cgcgtacata taaaactccg cttccgcgca
    40561 caccttttca atgattcaga gtgcgctata aatattcaca atagggcgcg aatattacgc
    40621 aaaacgcacg cctttgacaa gtgctacagt caatacacga agaaaaaaac acaacttgta
    40681 cggtaacgtt tatctgtgcc attttttcag tacaattacc ctatattcct aaccataaac
    40741 cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa aagcccggct tttgcgatga
    40801 atttagaaaa aatcaatgag ttaaccgcgc aagatatggc gggtgttaat gcggcaatcc
    40861 ttgagcagct taattccgac gtccaactga tcaatcagtt aggctattac atcgtcagcg
    40921 gcggcggtaa acgtattcgt ccgatgattg ctgtactggc tgcacgagct gttggctatg
    40981 agggaaatgc gcatgtcacc attgctgccc tgatcgagtt tatccacacg gcgactctgc
    41041 tacacgacga cgttgtggat gaatcagata tgcgcagggg taaagctacc gccaacgccg
    41101 catttggcaa tgccgccagc gtgctggtag gcgattttat ttatacccgc gcgttccaga
    41161 tgatgaccag cctcggttca ctcaaagtgc tggaagtcat gtcagaagct gtaaacgtca
    41221 tcgcagaagg tgaagttctg caactgatga acgttaacga tccggacatc actgaagaaa
    41281 actacatgcg cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc gcgcagtgtt
    41341 ccgggattct ggctggctgt acgccggagg aggagaaagg cctgcaggat tatgggcgct
    41401 atctcggcac tgctttccag ttgatcgacg atttactcga ttacaatgcc gatggcgaac
    41461 agttaggtaa aaacgtcggc gacgatctga acgaaggtaa accgacgctg ccgctgctgc
    41521 atgcgatgca tcatggcaca ccagaacagg cacagatgat ccgtaccgcc atcgaacaag
    41581 gtaacggtcg ccatcttctg gaaccggttc tggaagcaat gaacgcttgt ggatctcttg
    41641 aatggacgcg tcagcgcgcc gaggaagaag cagacaaagc catcgcagcg ttacaggtgc
    41701 tcccggacac cccttggcga gaagcactca tcggcctcgc gcacatcgct gttcaacgcg
    41761 atcgttaatc ccctcccttc atcccgcgca gtgcgcggga tgattccagt aaattccata
    41821 aaaacactaa ttcagcgcta taacacctga aaaaagggcg ataaatcttt atatattctg
    41881 aatattcaca ctctttacag gaacttttta gcgcaataga ccatcaggag tatagtgatg
    41941 cccgacagaa gaagtgttct gaatgaaagc gactaactta aggagtgagg aaagatggaa
    42001 agtaatttca ttgactggca tcccgctgac atcattgcag gtttgcgcaa gaaaggaacg
    42061 tcaatggcgg cggaatctcg cagaaatggt ttgagttcct caacgctggc aaacgcatta
    42121 tcgcgcccat ggccgaaagg agagatgatt attgcgaaag ccctgggaac tgacccctgg
    42181 gttatctggc catcacgcta ccatgatccg cagacccatg agtttatcga cagaacgcag
    42241 ttgatgcgta gctacactaa accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg
    42301 ggctgccagc tctcagacga ttactcgcct ttcacacgct caatatttgc acctaaagcg
    42361 cgcagtttgt cttcaatgcg ttcgtagcca cgatcgatgt gataaatacg atcaaccacc
    42421 gtcgtccctt ccgcaataca gccagccagc accaggcttg ctgatgcacg cagatcggtt
    42481 gccataacct gtgcaccaga aagtttttca acaccatgac aaataacggt attgctttcg
    42541 atttcggcgt gcgcgcccat acggctcagc tctggcacat gcataaagcg gttttcaaag
    42601 accgtttcgg tgatgaaccc ggtcccttca gccaccaggt tcaacagcgt gaactgggcc
    42661 tgcatatcgg tcgggaatgc cggatgcggc gcggtacgta cgttaacagc cttcggacgt
    42721 ttgccatgca tatccaggct aatccagtct tcgccgactt cgatgtccgc tccagcatca
    42781 cgcagtttcg ccagcacggc gtccagagta tctggctgcg cgttacggca gataattttg
    42841 ccacgagaga tcgccgccgc caccaggaaa gtaccggttt cgatacgatc cggcagcacg
    42901 cgatagacac cgccgcctaa acgttccaca ccttcgatga cgatacgatc ggtgccctga
    42961 ccgctaattt tcgcacccag cgtaatcagg aagttcgcgg tatcgacgat ttccggttca
    43021 cgcgctgcgt tttcaataat cgtggtgcct tccgccaggg ttgcagcaca catgatggtc
    43081 accgttgcgc caacgctgac tttatccatc acgatatgtg cgcctttcag acgaccatcg
    43141 acggaagctt taacgtaacc ttcttccagt ttgatggtcg cgcctaattg ttcgaggcca
    43201 gaaatgtgta gatcaaccgg acgcgcaccg atcgtacaac cgccaggtag tgaaacttgc
    43261 ccctgaccaa agcgcgctac cagcggcccc agcgcccaga tagaagcacg catggtttta
    43321 accagatcgt aaggtgcgca gaatacatta acgtcgcggg catcaatatg cacagaacca
    43381 ttacgttcta ctttcgcacc cagctggctt agcagtttca ttgatgtatc gacgtctttc
    43441 agtttcggga cgttctggat ctctaccggt tcttccgcca gtagtgcggc aaaaagaata
    43501 ggcagagcag catttttagc gccggaaatt gtgacttcgc cctggagctt cgttggcccc
    43561 tgaacacgaa atttatccat ttagtttgtt ctcagttaac aattcatatc cgctaccggc
    43621 gaatcgccca tagctcaaaa gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta
    43681 tacgctttga tcgacacagc atgaatgcgg ttatccgcaa tatattccat cagcgggcca
    43741 tagaccgtct gctgtttttt aacccgactc ataccgtcaa acaactcacc cacggcaata
    43801 acctgaaagt ggctgccatc gccggaaacg tggacttcct ggagggagag agcgttcatc
    43861 aacacgctct gaatttcatt attttccatg ggatcttcaa tcatcagtta ataaaccagc
    43921 gaaacatctt agagcaaagt tgcgctggca taaataagca aaaagcctcg ctgataaatc
    43981 agacaaggct cgacttgcag gcaggtttgc cggacaggcg gttaacgcca tatccggcct
    44041 gaaaaaattt aacgaggcag aacatcagca ggcaaattat acaatttcgc gagggtatac
    44101 actttgtcgt ttaccccctg aagcgtcacg ttgttgccct gctttttcgc cagatcgata
    44161 agatggagca gcagtgccag tccccccgta tccacgcggg acacacggct aagatcgatg
    44221 caggtaatcc ccttcaccgc ctcctcacgc atttcccaaa gcggtagcag aacgtcctga
    44281 tccagctctc cggataacgc cagcgtgtca cccgtctgca tccagctcag tgactcgctc
    44341 attatttttt ctcttccaga gtgattttct gttgagaaat cgatttcagt tgcgcagtca
    44401 ggccgtcgat acctttggta cgcagcagcg ttccccactc gttttgtttg gtggtgatca
    44461 tactgacgcc ttcagcaatc atgtcgtaag cttgccagtt tcccgtctgg gagtttttac
    44521 gccactggaa gtccagacgc accggcggac ggccattcgg gtcaataatg gtaacgcgaa
    44581 taggcacaat ggttttatcg cccagcggct gttctggcgc aatctgatag gtttgaccgt
    44641 gatacatcgc cagcgcctga ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt
    44701 aggcttcacg ttgagcaggg gtcgcactct tgtaatactg gcccagcacc agcgcaccgg
    44761 cgtatttcac ctgtacgtat ggcagcagtt cctgatcaac aatggtacgc agataatccg
    44821 ggttggcccg aatttgcggt tgctcattct tcaggcgatc gaacgttttc tgcgccgcct
    44881 cgtccatcag cttataagga ttggtctggt ctgccgcggt tgccgcactc agaggtgcaa
    44941 tcaccagcaa agcgaccatc attaaacgtt taaacatgcg tcggttctcc tgaaattatt
    45001 tcgttgtacc cacaggttca gtggtttcat tattacctgg cgcagcagct ggcgcatcgc
    45061 cactattctt attgtcatcg cctttactac cgtaaaggaa ctgaccaatg agatcttcca
    45121 gcaccatcgc agacttggtg tcctgaattg tatcgccatc cttcaggata gcagtcccca
    45181 gttccgggtc ttcaaaaccg acgtttaatg ccagatattg ttctcccagc aggccggaag
    45241 tacgaatgct cagcgaactg gtatcaggaa tgtggttata acgttgttca atttccagcg
    45301 ttacgcgcgg cagataggtt ttcgggtcca gcgtaatatc cgccacccgg cccacaacaa
    45361 caccaccaat gctgaccgga gagcgcgctt tcaggccgcc aatgttatcg aacgtcgcat
    45421 aaagcgtgta ggtcgattca gtacgtatgg acgtcacgtt cgccgccttc aggcaaacaa
    45481 acagcgccgc cagcagtgct gctaataaaa agatacccac ccaaatttca ttttttttcg
    45541 tttgcatgaa ctcaattccc aaacatcaat gcggtcagca caaaatccag ccccagaaca
    45601 gccagagacg agtggacaac ggtgcgagtg gttgcccggc taatcccggc agacgtcggg
    45661 atggcgtcgt agccgttaaa caacgaaatc cacgtcaccg tgatggcgaa caccacgctc
    45721 ttaatcagac agttgaccag atccatacgc cagtcgacgg cattttgcat tgccgaccag
    45781 aagaacccgc tatcaatgcc tttccaactg acgccgacca gcgatccgcc ccagatcccc
    45841 acggcgacga agataaccgt caacagtggt aatgaaataa ccccagccca gaaacgggga
    45901 gaaataaccc gacgcagcgg atccaccgcc atcatctcca tactggagag ttgctctgta
    45961 gcgcgcatca ggccgatttc tgcggttaac gccgaaccag cacgcccggc aaacaacagc
    46021 gcggcaacca ccggccccag ttcacgcagt agcgataacg ccaccagcat acccagactg
    46081 gtttccgcgc tataagtggt cagaaccaga taaccttgca gccccaacac cattccgatg
    46141 aacacgccag aaactacaat aatcagcatc gacaggacgc cgacattata gagctggcgc
    46201 accagcagcg gcgcatgttt gcgaaattcc ggtttgccga ccagcgcatt gaataacatt
    46261 aacccggccc gcccgaacgt tctcagggtt ttaatccctt tatgtccgag cgacgccagc
    46321 gcatttaaca gcatgagtgg cttaactccc tggtaaaaga tcagcgtgat aatcgccggc
    46381 aggatagcgg aacggaacag gcccgtcagc tatcccgtcc agaaactgac gtacgcgcgg
    46441 atcaggattc gtctgcaacg cctgggcact gccatgagcg acaatttttt tgtccgccag
    46501 gatccaggcg tgatccgcaa tacttaacac ttccggcaca tcgtgagaaa ccaccacaca
    46561 agtcacgccc agcgcgctgt tcagctcaga aatcagcttc accagtacgc ccatggtgat
    46621 gggatcttgc ccaacaaaag gttcatcaaa catgatgaga tccggctcca gcgcaatcgc
    46681 acgcgccagc gctgcacgcc gcgccatccc accggaaagt tcagaaggca ttagtttagc
    46741 cgctccacgc agccccacgg cctccagctt catcatcacc gtactatgca acaatggcgc
    46801 gggaagttgg gtatgttcgc gcagtggata ggcgacgttg tcaaatacgt tcatatcagt
    46861 gaacaacgcc ccggactgaa ataacatgct catccgttta cgcactgtat acaggcgcga
    46921 acgagacatc gccggaatat tctcaccatc gaaaaggatc tcaccatgat ctggtgcgat
    46981 ttgcccgcca atcagacgga gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc
    47041 cgtgatcttc cctcgcggca cggtcaggga aatattatcg aagatgcagc gattgccacg
    47101 cgtaaaactg acatcgcgca tatcgactaa attcgccaca gactgctcca taattcaccc
    47161 ttcgtcttgc gttgattttc taagcatggc gctcaattta accttgaacc caacatattt
    47221 acagaatatt acccgccgtg gttagcgaaa gctggcattt gttttacttt ttagccgcat
    47281 aaagtcaaaa ttaagaatcc gttacggctt tctgaaaatc ttcagcggac cggcgagtat
    47341 acctgaagaa aggacgttag atgcttttag ctacggcact gttaattgtt ggtttacttt
    47401 tggtcgttta cagtgccgac cgcctggttt ttgccgcgtc tattctttgc cgaacctttg
    47461 gcatcccgcc gctgatcatc ggcatgacgg tggtcagtat tggtacatcg ttaccagaaa
    47521 tcatcgtctc gcttgccgcg tctctgcacg aacaacgcga tttagccgtt ggtacagccc
    47581 tcggctcaaa cattatcaat atattgctga tcctcggtct ggctgcgctg gttcgtcctt
    47641 ttaccgtcca ttccgatgtt ctgcgccgtg aattaccctt aatgttgttg gtcagcgtgg
    47701 tggccggttc cgtactctat gacggacaac ttagtcgaag cgatggtatc tttctcctct
    47761 ttctggctgt gctatggctg ctgttcattg ttaaacttgc acgtcagggg actgacagcc
    47821 tgaccagaga gcagcttgca gagctgccgc gtgacggcgg attgcccgtc gcgtttttat
    47881 ggctcggcat tgcgcttatc atcatgccag tggccacgcg gatggtggtt gataacgcca
    47941 cggtgctggc gaattacttt gccatcagcg agttgacgat ggctctgacg gcaattgcta
    48001 tcggaaccag tctgccggaa ctggcaaccg caatagcggg ggttcgcaaa ggtgaaaacg
    48061 acattgctgt cggaaatatc attggcgcaa acatttttaa tattgtcatc gtgttgggtt
    48121 tacccgcgct gataacgcca ggagagattg atccactggc gtacagtcgt gactacagcg
    48181 tgatgttgct ggtgagcatt atttttgcgt tgctgtgctg gcggcgctcc ccgcaaccgg
    48241 gccgtggtgt aggggtatta ttaactggcg gatttatcgt atggctggcg atgttgtact
    48301 ggttatcgcc aatactcgtt gaataactgg aaacgcatta tgtcgcacgt agagttacaa
    48361 ccgggttttg actttcagca agcaggtaaa gaagtcctgg cgattgaacg tgaatgcctg
    48421 gcggagcttg atcaatacat caatcagaat ttcacgcttg cctgtgaaaa gatgttctgg
    48481 tgtaaaggga aagttgtcgt catggggatg gggaaatcgg ggcacatcgg gcgcaaaatg
    48541 gccgcaacgt ttgccagcac cggtacacct tcatttttcg tccatcctag tgaagccgcg
    48601 catggtgatt taggcatggt caccccacag gatgtggtga ttgctatctc taactcaggt
    48661 gaatccagcg aaatcacggc cttaattcca gtgcttaagc gtcttcacgt accgttaatc
    48721 tgcatcaccg gtcgcccgga gagcagcatg gcgcgcgccg cagatgtgca tctgtgtgtt
    48781 aaagtagcga aagaagcctg tccgttaggg ctggcaccga ccagcagcac caccgccacg
    48841 ctggttatgg gcgatgccct cgctgtcgcg ctgttaaaag cacgcggctt tactgctgaa
    48901 gattttgcgc tctcacaccc aggcggcgca ctgggtcgta aacttctgct gcgcgtaaac
    48961 gatattatgc atacgggcga tgagatcccg catgttaaga aaacggccag tctgcgtgac
    49021 gcattgctgg aagttacccg caaaaatctt ggtatgactg tcatttgcga tgacaatatg
    49081 atgattgaag gcatctttac cgacggtgat ttacgccgtg tcttcgatat gggcgtggat
    49141 gttcgtcagt taagtattgc cgatgtgatg acgccggggg gaatacgtgt gcgccctggc
    49201 attctggccg ttgaggcact gaacttaatg cagtcccgcc atatcacctc cgtgatggtt
    49261 gccgatggcg accatttact cggtgtgtta catatgcatg atttactgcg tgcaggcgta
    49321 gtgtaaagat tcaaggataa acaacaatga gcaaagcagg tgcgtcgctt gcgacctgtt
    49381 acggccctgt cagcgccgac gttatggcaa aagcagagaa cattcgtctg ctgatcctcg
    49441 acgttgatgg cgtactgtca gatggcctga tttatatggg caataacggc gaagagctga
    49501 aagcgttcaa tgttcgtgac ggttatggca ttcgttgtgc gctcacctct gatattgaag
    49561 tcgctatcat taccgggcga aaggctaaac tggtagaaga tcgttgtgcc acattgggga
    49621 tcactcactt gtatcagggg cagtcaaaca aactgatcgc ctttagcgat ttgctggaaa
    49681 aattggcgat tgccccggaa aatgtggctt atgttggcga tgatctcatc gactggccgg
    49741 taatggaaaa agtgggttta agcgtcgccg tggccgatgc gcatccactg ttgatcccgc
    49801 gcgccgatta cgtgacgcgc attgctggcg gtcgtggcgc agtgcgcgaa gtttgcgact
    49861 tattactcct ggcgcagggc aaactggatg aagccaaagg gcaatcgata tgagtaaagc
    49921 cagacgttgg gttatcattg tgctatcact ggcggttctg gtgatgatcg gcattaatat
    49981 ggccgaaaaa gacgataccg cccaggtggt cgtcaacaac aatgatccca cctataaaag
    50041 cgagcatacg gacacgctcg tctataaccc agaaggggca ctaagctatc gattgattgc
    50101 tcaacacgtt gaatattatt ccgatcaggc cgtttcgtgg tttacgcagc cggtacttac
    50161 cacgtttgat aaggataaaa tcccgacatg gtccgtaaaa gcagataaag ccaagctgac
    50221 caatgaccgg atgctctatt tatatggaca cgttgaagtc aacgcacttg tgccagactc
    50281 tcaacttcgc agaatcacga cggataacgc gcagatcaat ctggtgacgc aggatgttac
    50341 ctctgaagac ctcgtcacgt tatacggaac aacatttaac tccagcggtc tgaaaatgcg
    50401 cggcaactta cgcagcaaga acgccgagct gattgaaaag gttagaacat cctatgaaat
    50461 tcaaaacaaa caaactcagc cttaatcttg tgcttgccag ctcacttctg gccgccagca
    50521 ttccggcatt tgccgtaacc ggagacactg atcagccgat ccacattgaa tcggaccagc
    50581 aatctcttga tatgcaaggc aacgtggtta cctttaccgg taatgtcatc gtcacccagg
    50641 gcaccatcaa aattaatgcc gacaaagtgg tcgttacccg tccgggcggc gaacaaggta
    50701 aagaagtgat tgacggatac ggtaaaccgg caacgttcta ccagatgcag gacaacggta
    50761 aacccgttga aggtcacgct tcccagatgc actacgaact ggcaaaagat tttgtcgttc
    50821 tgacgggtaa tgcttatctg cagcaggtcg atagcaacat taagggcgat aagatcactt
    50881 acctggtgaa agagcagaaa atgcaggctt tcagcgacaa aggcaagcgc gtaaccaccg
    50941 ttctggtgcc gtcgcagctg caggacaaaa acaacaaagg ccagaccccg gcacagaaga
    51001 agggtaatta attcgttatg gcaacattaa ctgcaaagaa ccttgcaaaa gcctataaag
    51061 gccgtcgcgt ggtagaagac gtcagcctga ccgtcaactc cggggaaatt gtcggtctgc
    51121 tggggccaaa cggtgccggt aagaccacca ctttctacat ggttgtaggc attgtgccgc
    51181 gcgatgcggg caacatcatt attgatgatg acgatatcag tctgctgcct ctgcatgcac
    51241 gcgcgcgccg cggtatcggc tatctgccac aggaagcctc cattttccgt cgcctcagcg
    51301 tttacgataa cctgatggcg gtactgcaaa ttcgtgacga cttgtctgct gaacaacgtg
    51361 aagaccgcgc gaacgagctg atggaagagt ttcacattga gcacctgcgt gacagcatgg
    51421 ggcagtcact ctccgggggt gaacgtcgcc gtgtagaaat tgcccgcgca ctggctgcga
    51481 atccgaaatt tattctgctc gacgaaccgt ttgccggggt tgacccgatc tcggttatcg
    51541 acattaaacg catcattgaa cacctgcgcg acagcggcct gggcgtgctg atcactgacc
    51601 acaacgtgcg tgaaacactg gcggtttgtg aacgcgctta tatcgtcagt caggggcatt
    51661 tgatctccca cggcacgcct acagaaatct tacaagacga acacgttaag cgtgtatacc
    51721 ttggggaaga cttcagactc tgatagggta gaagtttgcg acgttttagc aggagagtac
    51781 gattctgaac atgaagcaag gtttgcaact caggcttagc caacaactgg cgatgacgcc
    51841 acagctccaa caggcaattc gtctgttgca gttgtcgacg ctggaacttc agcaggagct
    51901 acagcaggcg ctggagagta atccgctgct tgagcaaatc gacactcatg aagaaatcga
    51961 cacccgcgaa acgcaagaca gtgaaacgct ggacaccgcc gacgcgctcg aacaaaaaga
    52021 gatgccggaa gagctgccgc tcgatgccag ttgggacacc atttacaccg ctggtacacc
    52081 atccggcacc agcggtgact acattgacga cgagctgccg gtctaccagg gcgaaacgac
    52141 gcagaccttg caggattacc tgatgtggca ggtcgagctg acaccgtttt ccgacactga
    52201 ccgcgctatt gctacctcta tcgtcgatgc cgttgatgaa accggttatc tgactgtccc
    52261 gctggaagat attctcgaaa gtatgggcga tgaagagatc gacatcgacg aggttgaagc
    52321 cgtccttaag cggatccaac ggtttgaccc ggtcggtgta gcggcaaaag atctgcgtga
    52381 ctgcctgcta atccaactct cccaattcga taagaccacg ccgtggctgg aagaggccag
    52441 attgatcatt agcgatcatc tcgatctgtt agccaatcac gacttccgca ctttaatgcg
    52501 cgtcacgcgt ctgaaagaag atgtgctgaa agaagccgtc aatctgatcc agtcgctcga
    52561 tccgcgcccc gggcagtcga tccagactgg cgaacctgag tatgtcattc cagatgtgct
    52621 ggtgcgtaag cataacggtc actggacggt agaactcaac agtgacagca ttccgcgtct
    52681 gcaaatcaac cagcactacg cctcgatgtg caataacgcg cgcaacgatg gtgacagcca
    52741 gtttatccgc agcaatctgc aggatgccaa atggttgatc aagagtctgg aaagccgtaa
    52801 cgatacgcta ctgcgcgtga gtcgctgtat cgttgaacag cagcaagcct tctttgagca
    52861 aggtgaagaa tatatgaaac cgatggtact ggccgatatc gcccaggctg tcgaaatgca
    52921 tgaatcgacg atatctcgcg tgaccacgca aaaatacctg catagtccac gaggcatttt
    52981 tgaactgaag tatttctttt ccagtcacgt caataccgag ggcggcggcg aagcttcctc
    53041 cacggcgatt cgtgcactgg tgaagaaatt aatcgcggcg gaaaacccag cgaaaccgtt
    53101 gagcgacagc aagttaacct ctttgctgtc ggaacaaggt atcatggtgg cacgccgcac
    53161 tgttgcgaag taccgagagt ctttatccat tccgccgtca aaccagcgta aacagctcgt
    53221 ttgacccaac cgataaggaa gacactatgc agctcaacat taccggaaat aacgtcgaga
    53281 tcaccgaggc actgcgcgaa tttgttacag ctaaatttgc caaacttgag caatattttg
    53341 accgaatcaa ccaggtctat gttgttctga aagtggagaa agtcacccac acctcagatg
    53401 caacactgca tgtaaacggt ggtgaaattc atgccagcgc ggaaggtcag gacatgtacg
    53461 ctgccattga tggcttaatt gataagctgg cacgccagtt gaccaaacat aaagataaac
    53521 tgaaacaaca ctaattgtcc gggcaattag catgtgcatg gcggcctgtt gtgcggcaca
    53581 acgggccatt tgtacggtta atgctccgag cctgttccac tgtttgagtg ggcaggttct
    53641 taggtgaaat tatgacaaat aatgatacaa ctctacagct tagcagtgtt cttaacaggg
    53701 aatgtacgcg aagccgcgtc cactgtcaga gcaaaaaacg cgccctggaa atcatcagcg
    53761 agctggcggc gaaacaactt agcctgccgc ctcaggtggt ttttgaagct atcctgacgc
    53821 gggaaaaaat gggcagtacc ggtatcggca atggtattgc cattccgcat ggcaaacttg
    53881 aagaagatac tctgcgtgcc gttggcgttt tcgttcagct cgaaacgcct atagctttcg
    53941 atgccatcga caaccaaccg gtggacttac tttttgccct gctggtgccg gcagaccaaa
    54001 ctaaaacgca cctgcatact ctgtcgctgg tggcgaaacg tctggcggac aaaaccatct
    54061 gccgccgttt acgcgcagcc cagagcgatg aagagctgta tcaaatcatt acggataccg
    54121 aaggtactcc ggatgaagcg tagttattcg gtaatgtctc ttttagacgt tgtgaggaga
    54181 aacagtacat ggtactgatg atcgtcagcg gacgttcagg ttcaggtaaa tctgtcgccc
    54241 tgcgtgcgct ggaagatatg ggtttttact gcgtggataa ccttcccgta gtgttgttac
    54301 ccgatctggc tcgaactctg gccgatcgcg agatttctgc cgccgtcagc attgatgttc
    54361 gtaatatgcc ggagtcacca gaaatattcg aacaggcgat gagtaacctg cctgacgctt
    54421 tctcaccgca actactgttc ctggatgccg accgtaatac cttaattcgt cgttacagtg
    54481 acacgcgccg actgcatccg ctttccagca aaaacctgtc gctggaaagt gctatcgaca
    54541 aagaaagcga tttgctggag cctctgcgtt cgcgagcgga tctgattgtc gatacctcag
    54601 aaatgtccgt tcacgagctg gctgaaatgc tgcgtacccg tctgctgggt aaacgtgaac
    54661 gcgaactgac catggtcttt gagtctttcg gcttcaaaca cggtatccct atcgatgcag
    54721 attacgtctt tgacgtgcgc ttcttgccga acccgcactg ggatccgaaa ctgcgtccaa
    54781 tgacaggtct tgataaacct gtcgccgcgt tcctcgaccg ccacacagaa gtacacaatt
    54841 ttatctacca gacgcgaagc tatcttgagc tatggttacc tatgctggaa accaacaacc
    54901 gtagctacct gacggtcgcc attggttgta ccggcgggaa gcaccgttcg gtgtatattg
    54961 cagagcaact ggcagactac ttccgctcgc gcggtaaaaa cgtccagtca cgccatcgta
    55021 cgctggaaaa acgtaaacca tgaccgtcaa gcaaactgtt gaaatcacaa acaagctggg
    55081 catgcatgcc cggcctgcaa tgaagctgtt tgaattaatg cagggttttg acgctgaagt
    55141 gctgttacgt aatgacgaag gcaccgaggc tgaagccaac agcgttattg cgctgctgat
    55201 gttggattct gccaaaggac ggcagattga agttgaagcg accggtccac aggaagagga
    55261 agcactggcc gccgttatcg ccctctttaa ttctggtttt gatgaagatt aatcttcatc
    55321 actttgacat acaactccct tcaagctccc ccgacaataa gaaaatcacg tactgaaatc
    55381 gttctcaatc aacgtcattt gtacattttg tgcgcttttc actttcagaa gaaccttaag
    55441 aaaaccttaa gaggcattgt ttaggttttg tttaagttaa tcgaccatac tggagatcgt
    55501 cagaaaatat ttccaggaga tggcatgatt cgcttatctg aacaaagtcc cctgggcacc
    55561 ggaagacatc ggaaatgtta tgcgcatccg gaagatgccc aacgctgtat caagattgtc
    55621 taccatcgtg gtggcgataa agagatccgc cgtgagttaa agtactacgc gcatcttggt
    55681 cgccggttaa aagactggag tggaataccg cgctatcacg gtaccgttga gacggattgc
    55741 ggaaccggtt atgtctacga tgtgattgcc gattttgacg gcaagccttc cattacgctg
    55801 accgaatttg ctgaacaatg ccgttacgaa gaagacattg cacaactgcg ccagctactg
    55861 aaacagctga agcgttattt gcaggataac cgtatcgtga cgatgtcgct gaagccgcag
    55921 aatatcctct gtcaccgcat cagtgagtca gaggttatcc cggtcgtctg cgacaacatt
    55981 ggtgaaagca cattgattcc actggcgacc tggtcgaagt ggtgttgtct gcgtaagcaa
    56041 gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg ctattgccct gcagaaagat
    56101 ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc gcgaagctta atccagctgg
    56161 tgttgttgca taaacggctc accgcctaac tgatacatct gccgtaaaat ccacgcctga
    56221 cggctacgca cgtagcccga tggcgaggag actttgaaac gaagtggatt aggtaataca
    56281 gctgccagta atgcagcttc cgaccgggta agtttgctcg cgggtttgtg gaaataacgt
    56341 tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt cggcgatatt caggtaaacc
    56401 gtcaggatac gctttttgct ccagactgtt tctatcccca gcgttaatcc agcttccagc
    56461 ccttttcgta cccagctacg cccatcccat aaaaagagat tttggccgtc tgctgagaaa
    56521 tcgttgaagc accgcgaata cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa
    56581 tggaagcgac atcaaagccc cagtgctcag gaaatttctg atcttctgcg gcaatcaccg
    56641 ccagtcccat ccacggcgag atttgatcca tactgaccca gtcagaatgt gccacgtaac
    56701 gaaaattgcc atgcagccag gcgctgacct gtcgctcgac cattaccgct gagaagggaa
    56761 caggcgcaac gctaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta
    56821 accgcaaaag aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc
    56881 cagaaccagc acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg
    56941 cgccagcata tatgctgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc
    57001 gacaggacac ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc
    57061 agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat
    57121 acacataaaa ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc
    57181 acggtcaacg gtgcattcgc taccaagact ggcaaaattg cttaaattct tcgccgcgcc
    57241 aacccccccc ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg
    57301 gatttcaccg cgcgttattc gtgccgcttc aatcagcaca ttgcgcgttt ccgtcatcgc
    57361 ttcgccagtt aaatggttga taacatcaac ctgctgctta tccggtgcaa agcagaccgc
    57421 ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc
    57481 gtcatagacg ccgcatccgc tcagaattac gccaattttc ttcattgtga tcatcctttc
    57541 gcaattgact gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact
    57601 gattcatgta acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag
    57661 tgctgtgccc ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt
    57721 ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc
    57781 acgctttcag cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac
    57841 cgacgttatc ttcccaggcg ggaaggtcag gagactgaat ttgctgaccc agctgttgca
    57901 gatggcgtaa ccccactgac cccgccgcac ctttaatttt atgtccttcc tcaacaatgc
    57961 cttttttatc ctgcgccgtc agattcaact ccagcacgct gacatagccc ggcatcatct
    58021 tctcaaacac cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt
    58081 ccagcatggg aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac
    58141 tctcctcatc atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaaccg
    58201 aaagcggctt actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca
    58261 gcacgttagc ggttaaggcc accagcggcg gtaaatcctc gcgcggataa cgcttcgtca
    58321 gttcacgaga gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt
    58381 cgtattcgcc cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc
    58441 tgttacctaa tttttccagc acagaacgcg cgacaatcac gttcagttca atgtcttcca
    58501 ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct
    58561 cttctgccac cgacggtgcg tggatcgtca acgtaaaggt tgaacctttg ccctgttcac
    58621 tggtaaccgt aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa
    58681 taccggtgcc ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga
    58741 agattttatc cagctcatcc tgcggaatgc cgataccaga atcttccact tcaaaatgca
    58801 gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtgaatttga
    58861 cggcgttact gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct
    58921 gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca
    58981 atgcggagag attttccaga tcggcaagga agctggtgaa atcaaccggc tgattatcaa
    59041 gctggacctt gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca
    59101 gcgtgacggc cgaaacatgg atagttttga gatatttttc ctgctcggcg gtgagttcgg
    59161 tatccagcag aatgcggctc aggccgacga taccgttcag cggtgtacgc aattcgtgac
    59221 tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg ttcaagcgca tcctgatacc
    59281 gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt
    59341 cgtagtacgg cactttacgg atttcaaagc aggctttgcg cccgtccggg taatccagcc
    59401 actgttcata ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt
    59461 ttgcggcggc ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc
    59521 tttttccggt cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac
    59581 gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg taagaatgag gattgctgct
    59641 cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt
    59701 cctgcagttc tgcttccgct ttttcacgaa cggcaatttc ctgatttagc tgggcaatat
    59761 tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca
    59821 ccagccgtga cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt
    59881 agaccgccca cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt
    59941 cgacctgacc atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga
    60001 gggccagcgc cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat
    60061 aatactgcgc cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc
    60121 aataataccc aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc
    60181 aacgacctac tccgttggag gtagccacgg acgtcccagc gccgatccct gtacgccatg
    60241 ttcattcaga tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagcgg
    60301 agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg
    60361 cgtatggcga atcatttctc cggcagtgag cgtataatcc tccagttcaa taccgttcaa
    60421 acgcaccgct tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag
    60481 ctttatgccg tccacgctgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg
    60541 cccttcgcca ggcaggccga caattaagtg ggaacacacc ttcagcccgc gctgacgtgc
    60601 cagctgggtt gtacgctgat aacaggcaaa atcatggccg cggttgatgc gatgcagtgt
    60661 tttgtcgtgg gcggtttgta gccccagctc cagccacact tcgtaaccct ggtccttata
    60721 ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc gggcgggtac caacacacaa
    60781 accgacaata ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc
    60841 cgcaaaggtg ctggtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa
    60901 attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc tgctgcgctt catcggcaaa
    60961 cgaggcaaca ttacagaatg tgcagccacc acgcccgatg gtaccgtcac ggttagggca
    61021 gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc ccataacgac gggtgagatc
    61081 accaccaaac atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa
    61141 agaggccaaa gcctgccatt tttagctcaa ttcggcgatg acctgggtca atcgtcccgg
    61201 cctgctttta tcaactgcat aatcaatcaa aattaccgaa atttcatgca caatcacata
    61261 aatcactttt gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaaa
    61321 cagtagcatg aaacgtcatt accaattaag gcagtataaa atgctggttt tctcgtcagt
    61381 tcaaggcagg ataagggtta acacgccttt atgacagtca ggaattgact gtttctctaa
    61441 cgactctcct tttagcctta aagataaaat ctattttaat ttcagctatt taataaagaa
    61501 ttttgcgcta aagcacattt ctgtaccaat aagcttgcca tttgacctgt atcagctttc
    61561 ccgataagtt ggaaatccgc tggaagcttt ctggatgagc agcctgctca tcatatttat
    61621 gcagtaattg agatcccctc ttcaccgtat taaccgatgc gaaaaggaca acaagggggc
    61681 gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca cgctctttct gtgcccgtgc
    61741 gcagcggttc ggaagtgggg ttcccgcaga gcctggggga ggttcacgat atgttgtacg
    61801 ataaatccct tgagagggat aactgtggtt tcggcctgat cgcccacata gaaggcgaac
    61861 ctagccacaa ggtagtgcgt actgcaatac acgcactggc ccgcatgcag caccgtggcg
    61921 cgattctcgc cgatggtaaa accggcgacg gttgcggctt gctgttacaa aaaccggatc
    61981 gcttttttcg catcgttgcg caggagcgcg gctggcgttt agcaaaaaac tacgctgtcg
    62041 ggatgctctt cctgaataaa gatcctgaac tcgccgctgc cgcacgccgc atcgttgaag
    62101 aagaactgca acgcgaaacc ttgtcgattg tgggctggcg tgatgtcccc actaacgaag
    62161 gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat tgagcaaatt tttgtgaacg
    62221 ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct gtttatcgcc cgccgccgca
    62281 ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg tagcctgtcg aatctggtga
    62341 acatctataa aggtctgtgt atgccggcgg atctgccgcg cttttatctg gatcttgcgg
    62401 acctgcgtct ggaatcggcc atttgcctgt tccaccagcg cttctccact aacaccgtac
    62461 cgcgctggcc gctggcgcaa ccgttccgct atctggcgca taacggtgaa atcaacacca
    62521 tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa attccagacg ccgctgatcc
    62581 ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg ttctgactcc agttccatgg
    62641 ataacatgct ggaactgctg ctggcaggcg ggatggatat catccgcgcc atgcgcctgt
    62701 tagtgccacc cgcctggcag aacaacccgg atatggaccc ggaactgcgt gccttctttg
    62761 actttaactc catgcatatg gagccgtggg atggtccggc gggcatcgtg atgtccgatg
    62821 gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg tccggcacgc tacgtcatta
    62881 ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat ctgggattac cagcctgacg
    62941 aagtggtcga aaaaggccgc gtcgggccag gcgaactgat ggtgatcgac actcgcagtg
    63001 ggcgtattct gcactcggca gaaaccgatg acgatctgaa aagccgccac ccttataaag
    63061 agtggatgga gaaaaacgtc cgccgactgg taccgtttga agatctgccc gatgaagaag
    63121 tgggtagccg cgaactggac gacgacacgc ttgccagcta ccagaaacag tttaactaca
    63181 gcgcggaaga gctggactcc gtaattcgcg tactgggcga aaacggtcag gaagcggtcg
    63241 gttcgatggg cgatgatacc ccattcgccg tgctctccag tcagccgcgc attatttacg
    63301 actacttccg ccagcagttt gcccaggtga ctaacccgcc aatcgacccg ctgcgtgaag
    63361 cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat gaacgtcttt tgcgaagcag
    63421 agggccaggc gcaccgttta agctttaaat cgccgattct gctctactcc gatttcaaac
    63481 agctcacgac gatgaaagag gagcactacc gcgcagatac gctggatatc acctttgacg
    63541 tcactaaaac cacgctcgaa gcgacagtca aagagctatg cgacaaagcc gaaaaaatgg
    63601 tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa tatcgctaaa gatcgcctgc
    63661 cggttccggc tccgatggcg gttggcgcga tccagacccg tctggtcgat caaagcctgc
    63721 gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg cgatccgcac cacttcgccg
    63781 tgttgctggg tttcggcgcg acggctattt atccgtacct tgcctatgaa acgctgggcc
    63841 gcctggtaga cacccacgcg attgccaaag attatcgtac cgtgatgctc aactaccgta
    63901 acggcatcaa caaaggcttg tacaaaatca tgtccaaaat gggcatctcc accatcgcct
    63961 cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca cgatgatgta gtgggcctgt
    64021 gcttccaggg cgcggtcagc cgcattggtg gggcaagctt tgaagacttc cagcaggatc
    64081 tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc gattagccag ggcggcctgc
    64141 tgaaatacgt ccacggcggc gaataccacg cctacaaccc ggacgtggtg cgcacgctgc
    64201 aacaggcggt acaaagcggc gagtacagcg actatcagga atacgcgaag ctggttaatg
    64261 agcgtccggc aaccacgctg cgcgatctgc tggcaattac gccgggtgaa aacgcggtca
    64321 acattgctga tgttgaaccg gcaagcgaac tgtttaaacg ctttgatacc gccgcgatgt
    64381 ctatcggcgc gttaagcccg gaagcccacg aggcgctggc ggaagcgatg aatagcatcg
    64441 gcggtaactc gaactccggt gaaggcggcg aagacccggc gcgctatggc actaacaaag
    64501 tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt tactccggcg tatctggtca
    64561 atgccgacgt cattcagatt aaagtcgccc agggcgcgaa gccgggcgaa ggtggtcagt
    64621 tgccgggcga caaagtcact ccttacatcg ccaaactgcg ctattcggtg cccggagtga
    64681 cgctgatctc cccgccgccg caccacgata tctactctat cgaagactta gcgcagctta
    64741 ttttcgacct caagcaggtt aacccgaaag cgatgatctc cgttaagctg gtgtccgaac
    64801 caggcgttgg caccatcgcg actggcgtgg cgaaagctta tgcggacttg atcaccatcg
    64861 cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc agtgaaatat gcaggctgcc
    64921 cgtgggaact cggtctggtg gaaacccaac aggcgctggt tgccaacggc ttgcgtcata
    64981 aaattcgttt gcaggttgat ggcggcctga aaacgggtgt cgatatcatc aaagcggcga
    65041 ttctcggcgc agaaagcttc ggcttcggca ctggcccaat ggtggcgctc ggctgtaaat
    65101 atctgcgtat ttgccacctg aacaactgcg ccacgggtgt agcaactcag gatgacaaac
    65161 tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac taattacttt gagtttatcg
    65221 cccgtgaaac ccgcgagctg atggcacagc tgggagtaac gcgtctggtg gatctgattg
    65281 gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc caaacagcag aaactggcgc
    65341 tgtcgaagct gctggagact gccgagccac atgcaggtaa gtcactctac tgcactgaaa
    65401 acaacccgcc gtttgataac ggcctactga acgcgcagtt gctgcaacag gcgaaaccgt
    65461 ttgtcgatga acgccagagc aaaaccttct ggttcgatat ccgcaacacc gaccgttctg
    65521 tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg cgatcagggg ctggcagccg
    65581 atcctatcaa agcgtacttc aacggcaccg caggccagag tttcggtgtg tggaacgcgg
    65641 gcggcgtgga actgtacctg accggggatg ccaacgacta tgtcggtaaa ggcatggcgg
    65701 gcggcttaat cgccattcgt cctccggttg gttccgcctt ccgcagccat gaagcaagca
    65761 ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg tctgtatgcc gcaggccgcg
    65821 cgggtgaacg tttcggcgtg cggaactccg gtgctatcac cgtggtagaa ggcattggcg
    65881 acaacggctg tgaatatatg acgggcggta ttgtctgtat tctgggtaaa accggcgtta
    65941 acttcggcgc gggcatgacc ggcggcttcg cttacgttct cgatgaaagc ggtgatttcc
    66001 gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt cgacgatctg gcgatccatg
    66061 aagagcatct gcgcggtctt atcaccgagc atgttcagca taccggttct cagcgcggtg
    66121 aagagattct ggcgaactgg tcaaccttcg ccacgaaatt cgcgctggtt aaaccgaagt
    66181 ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag cgcagcagag ttgcgcgtgc
    66241 aggcgcagta aggggtagca acaatgagtc agaatgttta tcaatttatc gacctgcagc
    66301 gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat tgagtttgtt gaaatttacg
    66361 agccgttttc cgaaggccag gccaaagcgc aggctgaccg ctgtctgtcg tgcggcaacc
    66421 catactgcga gtggaaatgc ccggtacaca actacatccc aaactggctg aagctcgcca
    66481 acgaggggcg tatttttgaa gcggcagaac tgtcgcatca gaccaacaca ctgccggaag
    66541 tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg ttcctgcacc ctgaacgatg
    66601 agttcggtgc ggtgaccatc ggcaacattg agcgttatat caatgataaa gcgttcgaaa
    66661 tgggctggcg tccggatatg tccggcgtga agcaaaccgg taaaaaagtg gcgattatcg
    66721 gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac gcgtaacggc gtaaaagccg
    66781 ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac cttcggtatt ccggccttca
    66841 agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt caccggcatg ggtattgaat
    66901 tcaaactcaa taccgaagtg ggccgcgacg tacagctgga cgatctgctg agtgattacg
    66961 atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg cggcgggctg gaaaacgaag
    67021 acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc caacaccaaa cagttaatgg
    67081 gctttggtga aacccgcgac gaaccgttcg tcagcatgga aggcaaacgc gtggtagtac
    67141 ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc tgtacgccag ggggcaaagc
    67201 acgttacctg tgcctatcgt cgtgatgaag agaacatgcc gggttcccgc cgcgaagtga
    67261 aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt ccagccactg gggattgaag
    67321 tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg taccgaaatg ggcgaaccgg
    67381 atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc cgaacatatc gttccggcag
    67441 atgcggtgat catggcgttt ggtttccgtc cacacaacat ggaatggctg gcaaaacaca
    67501 gcgtcgagct ggattcacaa ggccgcatca tcgctccgga aggcaacgac aacgctttcc
    67561 agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt ccgtggttcc gatctggtcg
    67621 ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat tatgaactgg ctggaagttt
    67681 aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc gctgaaattc tcctttcttt
    67741 ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa tacgctcatc tggattgtta
    67801 ccggtagatg cccggcgtat agctgattcc attattcaac gcagcgccat ataaagggac
    67861 agataatgga atcgctctca gagagaacct caacaggcta ccagcaaatc cacaacggca
    67921 ttattcatct ggtcgatagc gcccgaacgg aaacggtacg tagcgttaac gcgctgatga
    67981 ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt tgaacaaggt ggtgaggcca
    68041 gagcagctta tggtgcacag ttgattgatc ggctatcgaa ggatttaagt cgacggtata
    68101 aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct tttttacctc ttttttcaac
    68161 atgttgaaat tcgccagaca gtgtctggcg aattaacacc attgggaatt ccgcagacac
    68221 catctgcaga attcccatcg gcaaagattt ggcagacgct gtctgccaaa ttgagaggct
    68281 gtttgatatt tccacagctt ttgtattgca tctcctattt tcggtttgta tattcatgca
    68341 aa
//
LOCUS       DBKELA010000005        67100 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.005, whole genome shotgun sequence.
ACCESSION   DBKELA010000005 DBKELA010000000
VERSION     DBKELA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.005"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="ACUQR0_000308"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="ACUQR0_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HHV9107499.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="ACUQR0_000309"
     CDS             complement(1150..1236)
                     /locus_tag="ACUQR0_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HHV9107500.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="ACUQR0_000310"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="ACUQR0_000310"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HHV9107501.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="ACUQR0_000311"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="ACUQR0_000311"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HHV9107502.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="ACUQR0_000312"
     CDS             3585..3629
                     /locus_tag="ACUQR0_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107503.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="ACUQR0_000313"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="ACUQR0_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HHV9107504.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="ACUQR0_000314"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="ACUQR0_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HHV9107505.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="ACUQR0_000315"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="ACUQR0_000315"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HHV9107506.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="ACUQR0_000316"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="ACUQR0_000316"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HHV9107507.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="ACUQR0_000317"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="ACUQR0_000317"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HHV9107508.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="ACUQR0_000318"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="ACUQR0_000318"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HHV9107509.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="ACUQR0_000319"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="ACUQR0_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HHV9107510.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="ACUQR0_000320"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="ACUQR0_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of D-glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HHV9107511.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="ACUQR0_000321"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="ACUQR0_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HHV9107512.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="ACUQR0_000322"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="ACUQR0_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HHV9107513.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="ACUQR0_000323"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="ACUQR0_000323"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HHV9107514.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="ACUQR0_000324"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="ACUQR0_000324"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HHV9107515.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="ACUQR0_000325"
     CDS             complement(16981..17745)
                     /locus_tag="ACUQR0_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9107516.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="ACUQR0_000326"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="ACUQR0_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HHV9107517.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="ACUQR0_000327"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="ACUQR0_000327"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HHV9107518.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="ACUQR0_000328"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="ACUQR0_000328"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HHV9107519.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="ACUQR0_000329"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="ACUQR0_000329"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HHV9107520.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="ACUQR0_000330"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="ACUQR0_000330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HHV9107521.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="ACUQR0_000331"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="ACUQR0_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HHV9107522.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="ACUQR0_000332"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="ACUQR0_000332"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HHV9107523.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="ACUQR0_000333"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="ACUQR0_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HHV9107524.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="ACUQR0_000334"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="ACUQR0_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HHV9107525.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="ACUQR0_000335"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="ACUQR0_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HHV9107526.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="ACUQR0_000336"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="ACUQR0_000336"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HHV9107527.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="ACUQR0_000337"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="ACUQR0_000337"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HHV9107528.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="ACUQR0_000338"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="ACUQR0_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HHV9107529.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="ACUQR0_000339"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="ACUQR0_000339"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HHV9107530.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="ACUQR0_000340"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="ACUQR0_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HHV9107531.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="ACUQR0_000341"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="ACUQR0_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HHV9107532.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="ACUQR0_000342"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="ACUQR0_000342"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HHV9107533.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="ACUQR0_000343"
     CDS             complement(40421..41815)
                     /locus_tag="ACUQR0_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9107534.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="ACUQR0_000344"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="ACUQR0_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HHV9107535.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="ACUQR0_000345"
     CDS             complement(42158..43444)
                     /locus_tag="ACUQR0_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHV9107536.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="ACUQR0_000346"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="ACUQR0_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="ACUQR0_000347"
     CDS             complement(44451..45221)
                     /locus_tag="ACUQR0_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HHV9107537.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="ACUQR0_000348"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="ACUQR0_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HHV9107538.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="ACUQR0_000349"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="ACUQR0_000349"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HHV9107539.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="ACUQR0_000350"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="ACUQR0_000350"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HHV9107540.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="ACUQR0_000351"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="ACUQR0_000351"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HHV9107541.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="ACUQR0_000352"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="ACUQR0_000352"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HHV9107542.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="ACUQR0_000353"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="ACUQR0_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HHV9107543.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="ACUQR0_000354"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="ACUQR0_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HHV9107544.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="ACUQR0_000355"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="ACUQR0_000355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HHV9107545.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="ACUQR0_000356"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="ACUQR0_000356"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HHV9107546.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="ACUQR0_000357"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="ACUQR0_000357"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HHV9107547.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="ACUQR0_000358"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="ACUQR0_000358"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HHV9107548.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="ACUQR0_000359"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="ACUQR0_000359"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HHV9107549.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="ACUQR0_000360"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="ACUQR0_000360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHV9107550.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="ACUQR0_000361"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="ACUQR0_000361"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HHV9107551.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="ACUQR0_000362"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="ACUQR0_000362"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HHV9107552.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="ACUQR0_000363"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="ACUQR0_000363"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HHV9107553.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="ACUQR0_000364"
     CDS             complement(66187..66405)
                     /locus_tag="ACUQR0_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HHV9107554.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="ACUQR0_000365"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="ACUQR0_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HHV9107555.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16290 a        18261 c        17267 g        15282 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaacataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct
//
LOCUS       DBKELA010000006        66027 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.006, whole genome shotgun sequence.
ACCESSION   DBKELA010000006 DBKELA010000000
VERSION     DBKELA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.006"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(123..602)
                     /gene="folK"
                     /locus_tag="ACUQR0_000366"
     CDS             complement(123..602)
                     /gene="folK"
                     /locus_tag="ACUQR0_000366"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HHV9107556.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(599..1996)
                     /gene="pcnB"
                     /locus_tag="ACUQR0_000367"
     CDS             complement(599..1996)
                     /gene="pcnB"
                     /locus_tag="ACUQR0_000367"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HHV9107557.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2056..2952)
                     /gene="gluQRS"
                     /locus_tag="ACUQR0_000368"
     CDS             complement(2056..2952)
                     /gene="gluQRS"
                     /locus_tag="ACUQR0_000368"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HHV9107558.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(3019..3474)
                     /gene="dksA"
                     /locus_tag="ACUQR0_000369"
     CDS             complement(3019..3474)
                     /gene="dksA"
                     /locus_tag="ACUQR0_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HHV9107559.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3652..4356)
                     /gene="sfsA"
                     /locus_tag="ACUQR0_000370"
     CDS             complement(3652..4356)
                     /gene="sfsA"
                     /locus_tag="ACUQR0_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HHV9107560.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4371..4901)
                     /gene="thpR"
                     /locus_tag="ACUQR0_000371"
     CDS             complement(4371..4901)
                     /gene="thpR"
                     /locus_tag="ACUQR0_000371"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HHV9107561.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4975..7404
                     /gene="hrpB"
                     /locus_tag="ACUQR0_000372"
     CDS             4975..7404
                     /gene="hrpB"
                     /locus_tag="ACUQR0_000372"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HHV9107562.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7600..10134
                     /gene="mrcB"
                     /locus_tag="ACUQR0_000373"
     CDS             7600..10134
                     /gene="mrcB"
                     /locus_tag="ACUQR0_000373"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HHV9107563.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10354..12597
                     /gene="fhuA"
                     /locus_tag="ACUQR0_000374"
     CDS             10354..12597
                     /gene="fhuA"
                     /locus_tag="ACUQR0_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HHV9107564.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12648..13445
                     /gene="fhuC"
                     /locus_tag="ACUQR0_000375"
     CDS             12648..13445
                     /gene="fhuC"
                     /locus_tag="ACUQR0_000375"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HHV9107565.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13445..14335
                     /gene="fhuD"
                     /locus_tag="ACUQR0_000376"
     CDS             13445..14335
                     /gene="fhuD"
                     /locus_tag="ACUQR0_000376"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HHV9107566.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14332..16314
                     /gene="fhuB"
                     /locus_tag="ACUQR0_000377"
     CDS             14332..16314
                     /gene="fhuB"
                     /locus_tag="ACUQR0_000377"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HHV9107567.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16349..17629)
                     /gene="hemL"
                     /locus_tag="ACUQR0_000378"
     CDS             complement(16349..17629)
                     /gene="hemL"
                     /locus_tag="ACUQR0_000378"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HHV9107568.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17795..17842
                     /gene="yadX"
                     /locus_tag="ACUQR0_000379"
     CDS             17795..17842
                     /gene="yadX"
                     /locus_tag="ACUQR0_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HHV9107569.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17854..19275
                     /gene="clcA"
                     /locus_tag="ACUQR0_000380"
     CDS             17854..19275
                     /gene="clcA"
                     /locus_tag="ACUQR0_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HHV9107570.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19299..19364
                     /gene="yadW"
                     /locus_tag="ACUQR0_000381"
     CDS             19299..19364
                     /gene="yadW"
                     /locus_tag="ACUQR0_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HHV9107571.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19357..19701
                     /gene="erpA"
                     /locus_tag="ACUQR0_000382"
     CDS             19357..19701
                     /gene="erpA"
                     /locus_tag="ACUQR0_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HHV9107572.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19748..20371)
                     /locus_tag="ACUQR0_000383"
     CDS             complement(19748..20371)
                     /locus_tag="ACUQR0_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HHV9107573.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20409..21209)
                     /gene="btuF"
                     /locus_tag="ACUQR0_000384"
     CDS             complement(20409..21209)
                     /gene="btuF"
                     /locus_tag="ACUQR0_000384"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HHV9107574.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21202..21900)
                     /gene="mtnN"
                     /locus_tag="ACUQR0_000385"
     CDS             complement(21202..21900)
                     /gene="mtnN"
                     /locus_tag="ACUQR0_000385"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HHV9107575.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21984..23501
                     /gene="dgt"
                     /locus_tag="ACUQR0_000386"
     CDS             21984..23501
                     /gene="dgt"
                     /locus_tag="ACUQR0_000386"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HHV9107576.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23631..25055
                     /gene="degP"
                     /locus_tag="ACUQR0_000387"
     CDS             23631..25055
                     /gene="degP"
                     /locus_tag="ACUQR0_000387"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HHV9107577.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25210..26366
                     /gene="cdaR"
                     /locus_tag="ACUQR0_000388"
                     /pseudo
     CDS             25210..26366
                     /gene="cdaR"
                     /locus_tag="ACUQR0_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26455..26841)
                     /locus_tag="ACUQR0_000389"
     CDS             complement(26455..26841)
                     /locus_tag="ACUQR0_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HHV9107578.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27155..27979)
                     /gene="dapD"
                     /locus_tag="ACUQR0_000390"
     CDS             complement(27155..27979)
                     /gene="dapD"
                     /locus_tag="ACUQR0_000390"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HHV9107579.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28010..30682)
                     /gene="glnD"
                     /locus_tag="ACUQR0_000391"
     CDS             complement(28010..30682)
                     /gene="glnD"
                     /locus_tag="ACUQR0_000391"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HHV9107580.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30744..31538)
                     /gene="map"
                     /locus_tag="ACUQR0_000392"
     CDS             complement(30744..31538)
                     /gene="map"
                     /locus_tag="ACUQR0_000392"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HHV9107581.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31906..32631
                     /gene="rpsB"
                     /locus_tag="ACUQR0_000393"
     CDS             31906..32631
                     /gene="rpsB"
                     /locus_tag="ACUQR0_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HHV9107582.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32889..33740
                     /gene="tsf"
                     /locus_tag="ACUQR0_000394"
     CDS             32889..33740
                     /gene="tsf"
                     /locus_tag="ACUQR0_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HHV9107583.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33887..34612
                     /gene="pyrH"
                     /locus_tag="ACUQR0_000395"
     CDS             33887..34612
                     /gene="pyrH"
                     /locus_tag="ACUQR0_000395"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HHV9107584.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34904..35461
                     /gene="frr"
                     /locus_tag="ACUQR0_000396"
     CDS             34904..35461
                     /gene="frr"
                     /locus_tag="ACUQR0_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HHV9107585.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35553..36749
                     /gene="ispC"
                     /locus_tag="ACUQR0_000397"
     CDS             35553..36749
                     /gene="ispC"
                     /locus_tag="ACUQR0_000397"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HHV9107586.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36938..37696
                     /gene="ispU"
                     /locus_tag="ACUQR0_000398"
                     /gene_synonym="uppS"
     CDS             36938..37696
                     /gene="ispU"
                     /locus_tag="ACUQR0_000398"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     ditrans,polycis-undecaprenyl-diphosphate synthase
                     [(2E,6E)-farnesyl-diphosphate specific] activity [Evidence
                     IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HHV9107587.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37709..38566
                     /gene="cdsA"
                     /locus_tag="ACUQR0_000399"
     CDS             37709..38566
                     /gene="cdsA"
                     /locus_tag="ACUQR0_000399"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HHV9107588.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38578..39930
                     /gene="rseP"
                     /locus_tag="ACUQR0_000400"
     CDS             38578..39930
                     /gene="rseP"
                     /locus_tag="ACUQR0_000400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HHV9107589.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39960..42392
                     /gene="bamA"
                     /locus_tag="ACUQR0_000401"
     CDS             39960..42392
                     /gene="bamA"
                     /locus_tag="ACUQR0_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HHV9107590.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42514..42999
                     /gene="skp"
                     /locus_tag="ACUQR0_000402"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42514..42999
                     /gene="skp"
                     /locus_tag="ACUQR0_000402"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HHV9107591.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43003..44028
                     /gene="lpxD"
                     /locus_tag="ACUQR0_000403"
     CDS             43003..44028
                     /gene="lpxD"
                     /locus_tag="ACUQR0_000403"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HHV9107592.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44133..44588
                     /gene="fabZ"
                     /locus_tag="ACUQR0_000404"
     CDS             44133..44588
                     /gene="fabZ"
                     /locus_tag="ACUQR0_000404"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HHV9107593.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44592..45380
                     /gene="lpxA"
                     /locus_tag="ACUQR0_000405"
     CDS             44592..45380
                     /gene="lpxA"
                     /locus_tag="ACUQR0_000405"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HHV9107594.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45380..46528
                     /gene="lpxB"
                     /locus_tag="ACUQR0_000406"
     CDS             45380..46528
                     /gene="lpxB"
                     /locus_tag="ACUQR0_000406"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HHV9107595.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46525..47121
                     /gene="rnhB"
                     /locus_tag="ACUQR0_000407"
     CDS             46525..47121
                     /gene="rnhB"
                     /locus_tag="ACUQR0_000407"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HHV9107596.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47158..50640
                     /gene="dnaE"
                     /locus_tag="ACUQR0_000408"
     CDS             47158..50640
                     /gene="dnaE"
                     /locus_tag="ACUQR0_000408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HHV9107597.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50653..51612
                     /gene="accA"
                     /locus_tag="ACUQR0_000409"
     CDS             50653..51612
                     /gene="accA"
                     /locus_tag="ACUQR0_000409"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HHV9107598.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51711..53852
                     /gene="ldcC"
                     /locus_tag="ACUQR0_000410"
     CDS             51711..53852
                     /gene="ldcC"
                     /locus_tag="ACUQR0_000410"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HHV9107599.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53909..54298
                     /locus_tag="ACUQR0_000411"
     CDS             53909..54298
                     /locus_tag="ACUQR0_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHV9107600.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54363..55661
                     /gene="tilS"
                     /locus_tag="ACUQR0_000412"
     CDS             54363..55661
                     /gene="tilS"
                     /locus_tag="ACUQR0_000412"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HHV9107601.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55710..55970)
                     /gene="rof"
                     /locus_tag="ACUQR0_000413"
     CDS             complement(55710..55970)
                     /gene="rof"
                     /locus_tag="ACUQR0_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HHV9107602.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55957..56157)
                     /locus_tag="ACUQR0_000414"
     CDS             complement(55957..56157)
                     /locus_tag="ACUQR0_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HHV9107603.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56323..56868
                     /locus_tag="ACUQR0_000415"
     CDS             56323..56868
                     /locus_tag="ACUQR0_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HHV9107604.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56865..57287
                     /gene="arfB"
                     /locus_tag="ACUQR0_000416"
     CDS             56865..57287
                     /gene="arfB"
                     /locus_tag="ACUQR0_000416"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HHV9107605.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57301..58011
                     /gene="nlpE"
                     /locus_tag="ACUQR0_000417"
                     /gene_synonym="cutF"
     CDS             57301..58011
                     /gene="nlpE"
                     /locus_tag="ACUQR0_000417"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HHV9107606.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKTVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58166..58990)
                     /locus_tag="ACUQR0_000418"
     CDS             complement(58166..58990)
                     /locus_tag="ACUQR0_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HHV9107607.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59043..60761)
                     /gene="proS"
                     /locus_tag="ACUQR0_000419"
     CDS             complement(59043..60761)
                     /gene="proS"
                     /locus_tag="ACUQR0_000419"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HHV9107608.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60872..61579)
                     /gene="tsaA"
                     /locus_tag="ACUQR0_000420"
     CDS             complement(60872..61579)
                     /gene="tsaA"
                     /locus_tag="ACUQR0_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HHV9107609.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61576..61980)
                     /gene="rcsF"
                     /locus_tag="ACUQR0_000421"
     CDS             complement(61576..61980)
                     /gene="rcsF"
                     /locus_tag="ACUQR0_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HHV9107610.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62098..62913)
                     /gene="metQ"
                     /locus_tag="ACUQR0_000422"
     CDS             complement(62098..62913)
                     /gene="metQ"
                     /locus_tag="ACUQR0_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HHV9107611.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62953..63606)
                     /locus_tag="ACUQR0_000423"
     CDS             complement(62953..63606)
                     /locus_tag="ACUQR0_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HHV9107612.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63599..64630)
                     /gene="metN"
                     /locus_tag="ACUQR0_000424"
     CDS             complement(63599..64630)
                     /gene="metN"
                     /locus_tag="ACUQR0_000424"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HHV9107613.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64818..65393
                     /gene="gmhB"
                     /locus_tag="ACUQR0_000425"
     CDS             64818..65393
                     /gene="gmhB"
                     /locus_tag="ACUQR0_000425"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HHV9107614.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65757..>66027
                     /locus_tag="ACUQR0_000426"
     rRNA            65757..>66027
                     /locus_tag="ACUQR0_000426"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15624 a        16348 c        18198 g        15857 t
ORIGIN      
        1 ttgatattta tctgcgggaa tttatattat gtttctgcaa aaatgtttaa actaaaattt
       61 ttattaagga aagagaggat gtatgatttt cagaaaatat ttatattcgt aatataaata
      121 aattaccatt tgcttaattt gtcaaatgct cttgtatgta agacttcacg caacgtctcc
      181 ccatcaggaa acgccagctc cggtgcaatc tcaaacagcg gccacagcat aaatccacga
      241 ttcttcatat cgtagtgcgg aacggtcagg cgttcggtat ttatcacttc attaccaaac
      301 agcatgatat cgagatccag cgtgcgtggt ccccagcgtt cagctttacg aacacgaccc
      361 tgttgcagtt ctatgcgctg tgtatggttg agtaactctt ccggggcggg cgaagtttca
      421 agcgccacgg cggcgttgag gtaatcgggt tgatcttgcg gccccagcgg tggggtgcgg
      481 taaaacgaag aaacggcaag aatgcggctt tcagggatat cgcctaatgc tttcagggca
      541 gcattgacct gctccagcgg agaggccaga ttgctgccta tggcaatata cgccactgtc
      601 atgtggtacc ctcacgacgc ggtgcgcgtt tgcgtggacg acgagtacga cgacgcggtg
      661 acggttcttc atccagctcg ttaagcatcc ctttttgatc tggtggtgcg gaaacctgga
      721 actcacccca ccatttcacc agacgctgca gttcagcatt acgttcaact tcagctcgca
      781 aggccaacag gtcataagcc gcacggaact taggatgctc cagcagtttc catgcgcgtt
      841 taccctgacg acgggacata cgcaactgca actgccagat atcgcgggtt aacgtcgtca
      901 gacgtttcgg gattgccagt gaacggcagg cttcgtccag cacgtcgttc atcgccagcg
      961 cgaaagcgtc gtgataggtc aggccgcttt cctgggcgat cttctgtgcc gtctccagca
     1021 gtgggtacca gaacatggcg gcaaacaaga acgccgggtt cacgcgcata tcgttatgga
     1081 tacgcgtatc ggtattcttc agcacctgtt caatgatccg ctccatcggg ctgtcgccat
     1141 tttccgtgaa gtagcgggta atggtcggga acagcggctg gaacagatga tattcacaca
     1201 acagcttata ggtttcgtaa ccgtagcccg cttgtagcag tttaagcgat tcttcaaaca
     1261 ggcgtgccgg tgggatatcg ttcagcaggg tagcgaggcg agggatcggt tctgcggttt
     1321 ccgggctgat gcgcataccc aatttggcgg caaaacgtac cgcgcgcagc atacgtaccg
     1381 gatcttcacg gtagcgcgtt tccgggttac caatcagacg gataacgccg tccttcagat
     1441 ccttcatacc accaacgtaa tcacggacgg taaaatccgc tacgctgtaa tacaggctgt
     1501 tgatagtgaa atcgcggcgc tgggcgtctt cttcgatgga gccgaaaatg ttgtcgcgca
     1561 gcaacatgcc gttttgcccg cgttgggagg tcgtgcggtc gctgacgtta ccttcgtggt
     1621 gtccacggaa ggtcgcaact tcgataatct ccgggccaaa cattacatga gccagacgga
     1681 aacggcgacc caccaggcgg cagttacgga acagtttgcg cacctgctca ggcgtggcgt
     1741 tagtggttac gtcaaaatct ttcggctttt tgccaagtaa caggtcgcgc acgccgccgc
     1801 caaccagcca ggcttcgtat cccgctttgt tgagcctgta cattaccttc agggcatttt
     1861 cactgatatc tttgcgggaa atagcatgct gctcacgcgg gatcaccgtc acctgtggac
     1921 gggcgactgc ctgttcagcc tcgctttcct cgcggcttag caccttgcgg caaaaattag
     1981 cgactcgggt aaaaatagta cacctcggta gtgtcaaaca tcattcagga caaaaaaata
     2041 gcggctaatc atagctcagc atgacgcatt tgagaatgtt gaatttacaa ttgccgactc
     2101 gggcacggcg gtcagcatcc agtttttgac ggctgactga aggatttgct cgacgctgaa
     2161 atcctgccag tgtgcttctg cctgctgccc cagaaattga agtgccgcga ttagtaccgg
     2221 gcgtggatcg cctttcggca acgcaggcgc atgattctgc ttggaaagtt tagcgccttg
     2281 tggattaagc gccagcggca gatgaatgta atcaggcact ttccagccaa aaagctggta
     2341 cagcgggatt tgccttactg ttggttcaat cagatcagcc ccacgcacta tttctgtaac
     2401 gccctggaaa tgatcatcaa ccacaacagc caggttgtag gcgaacaacc catcacggcg
     2461 atgaatgata aaatcttccc gtgccagttt ttcgtcggcg tgaataatgc ctcgcagcag
     2521 gtcagtaaat tgcgtgaccg gatgctgctg gcggatacgc actgcggcgt tgtctggtcc
     2581 atgatgcaac acccggcaat gaccgtcgta aataccgcca atgctttgaa tacgcgcacg
     2641 cgtacaggtg cagtaataac ttagtccttg ttcatgtaac caggcgagtg cttcacgata
     2701 ggcgtcgtga cgttgcgatt gccagagaac atcgccgtcc cagtgcagac cgtaatgttc
     2761 cagctggcgc aggatagttt ctgcggcacc gggaacttca cgaggcgggt cgatatcttc
     2821 tatgcgtacc agccagcgac cttgccgggc gcgagcctgc aaatagctgc cgagcgcggc
     2881 gatcagagag ccaaaatgaa gctcgccgga aggagagggg gcgaagcggc caatatactg
     2941 tgtgtctgtc atctctttga acaaaaaata aggcgggagc atttcccgcc tgtggtaaac
     3001 gtgatggaac ggctgtaatt agccagccat ctgtttttcg cgaatttcag ccagcgtttt
     3061 gcagtcgatg cacagatcgg ctgtcgggcg cgcttccaga cggcgaatac caatttcaac
     3121 accgcaggat tcgcagtagc cgaaatcttc gtcttccact tttttcagcg tcttctcgat
     3181 ctttttgatc agcttacgct cgcgatcgcg gttacgcagt tcgaggctga actcttcttc
     3241 ctgggctgca cggtctaccg ggtccgggaa gttggctgct tcatcctgca tatgtgtaac
     3301 ggtgcgatcg acttcatccc tgagttgatt acgccatgct tccagaatac gacggaagtg
     3361 cgccagctgg gcttcattca tatactcttc gcccggcttc tcctgatatg gttccacccc
     3421 agcgatggcg agaatactca gggacgatgt tttacggttt tgcccttctt gcatgttgct
     3481 tctccttaac acgcactatc gatccccatg ttcgggggaa aaatgaggcc gctataaata
     3541 gcagatgctt ttccggatag caattatcta aacgtaacac ttgacaactg tgtgaggaaa
     3601 agcgtatttg cgcacgcgac cagaatgtaa attaaccagt tacttacttt actacaatgt
     3661 aaccggcagt gattttttaa gagccatgcc ttcagcagaa atttccgctt tgtaagccag
     3721 aatttctacc cccctctgtt gagcttctga caatagttgc gcgtatttct catcgatgtg
     3781 gcgcgcgggt gaaaaccgtg taatggctga atgcagcacg gcgaaaaaaa taaccgcacg
     3841 ctggccttca gccgctacgc tcatcaactc ccgaaggtgt ttctgacctc gttcagtgac
     3901 cgcatcggga aaatatccct gttcgttctc cgctaacgta accgatttca cttcaatata
     3961 gcagtctgga cgcgaatccg cctgcaacat aaagtcaata cggctgcgtt cggagccgta
     4021 ttttacttcg cttttcagcg agctatagcc tgacagttct gaaattgatt cattaaggat
     4081 agcctctttc gtcaacctgt tagcccaaag cgtattgacg caaataaatg cgccgctctg
     4141 gctttgagtt aattcccagg tgtgtgggta tttccgtttg gtgttgtctg aagtcgaata
     4201 ccagacggta tcgccaggcg ttgcacaacc ggtcatcgca cccgtattcg ggcagtgtag
     4261 cgttaattcg cgaccatcgg gtgtgatcac atcggctaaa aaacgtttgt aacgctgaat
     4321 tagcgtcgcg cgctgtagag ggggagaaaa ttccattgcg acaatccttg ttattgcgtt
     4381 agcgcccagc gttttagcgg cgtgtagcgt gtgcgtccac gggcaaacga ggaggcgtaa
     4441 agggtgaact ccgtcaccgc ataagaccag ttaaaacccg gcggcgggaa tgtcaccgcc
     4501 tcgctggcgt cgcgcaataa ggtaatatgt ggatgaaacg gacgattgct ttgaaaacaa
     4561 ccgctgcggg cagcctgtga acggagcata ttcgccagct ggattaagcc gcgtggaggc
     4621 tgacgcatcc ctaaccacac cacacgcgaa cgcagccatt gtccggcgtc atcaagcgtg
     4681 agtgtgaaac caggttgacg aatccgtccg gctaaaagag aaagcgcctt ctctttctct
     4741 gcgctcactt cgcctaaaaa tgccagagtc aggtgcaaat tatcggcggc gaccggacgt
     4801 cccgcctcag gtgggaagtg tgtggcgcgc caatggataa tctgttcgcg gatttctgca
     4861 ggtaagtcga tagcaaagaa cagacgttgc ggttcagaca tgtgaggcac tcggttatga
     4921 attaccgcga tgctacaatg tggcgcgaag aatgttaacc ctctggagcg ttttgtgtcg
     4981 tcgttgcccg ttgctgccgt cttacctgaa ttacttaccg ctctcgattg tgcgccgcag
     5041 gtattattaa gtgcgccgac cggggccggg aaatcaacct ggctgccgct gcaactgctg
     5101 gcgcatcccg gcattaacgg gaaaattatc ctgctggagc cgcgtcgtct ggcggcgcgt
     5161 aacgtcgcgc aacggctggc ggagctgctt aacgaaaagc caggcgatac cgttggctac
     5221 cggatgcgtg cgcaaaactg cgtcgggctg aatacccgcc tggaagtggt taccgaaggc
     5281 gtgctgacgc ggatgatcca gcgtgacccg gaactgagcg gtgttggatt ggtgatcctt
     5341 gatgagtttc atgagcgcag tttgcaggcg gatttggcgt tggcgctgtt actcgatgtg
     5401 caacaaggtc tgcgtgatga ccttaaactg ctgattatgt cggctacgct ggacaatgac
     5461 cgcttgcagc aaatgctgcc ggaagcgccc gtcgtcatct cagaagggcg ctcgtttccg
     5521 gttgaacgcc gttatttacc gctgcccgcg catcagcgtt ttgacgaagc cgttgcggta
     5581 gccaccgccg aaatggtgcg tcaggaaagt ggatcattac tgttattttt acctggcgtc
     5641 ggagaaattc agcgcgtgca ggaacaactg gcttcgcgca tcggcagtga tgtggtgctc
     5701 tgcccgctgt atggcgtgtt gtcactgaac gatcagcgaa aagcgatcct cccggcaccg
     5761 caagggatgc gcaaagtggt gctggcgacc aatattgctg aaaccagttt aaccatcgaa
     5821 ggtattcgtc tggtggtgga ttgtgcccag gagcgtgtgg cgcgctttga tccgcgcacg
     5881 gggcttacgc ggttgattac tcaacgcgtt agtcaggcat ccatgacgca gcgtgccggg
     5941 cgcgccgggc gtctggagcc gggtatcagc ctgcatttaa tcgccaaaga acaagcagaa
     6001 cgcgccgcgg cgcaaagtga gccggagatc ttacaaagcg atctttccgg tttgctgatg
     6061 gaattactgc aatggggatg cagcgatccg gcgcagatga gctggctgga tcaaccgcca
     6121 acggtgaatc tactggccgc gaaacgcctg ttacaaatgc tgggggcgct ggagggtgaa
     6181 cggcttagtg cgcaagggca aaaaatggca gcgctgggta acgatccgcg tttagcggca
     6241 atgctggtta acgcaaagag cgatgacgaa gctgctaccg cggcaaaaat tgccgccatt
     6301 ctcgaagagc cgccacggat gggcaatagt gacctgggcg tggcgttttc gcgcaatcaa
     6361 ccagcctggc agcaacgtag tcagcaactg ttaaaacgct taaacgtacg cggcggtgag
     6421 gcagacagtt cgcttatcgc gccgctactt gccggggcgt ttgccgatcg cattgctcat
     6481 cgccgtgggc aagatggacg ctatcaactg gcaaacagca tgggagcgat gctcgatgcc
     6541 gacgacgcgc taagccgcca cgaatggttg atcgcaccgt tattattgca gggcagcgcc
     6601 tcgccggatg cgcggatttt actggcgcta ccggtcgata ttgatgaatt agtacaacgc
     6661 tgtccgcagc tggtgcagca atctgacacc gtggaatggg atgacgcgca aggtacgctg
     6721 aaagcctggc gtcggctaca aatcggtcag ttgacggtga aagtgcagcc gctggcgaaa
     6781 ccgtcagaag acgagttgca tcaggcgatg cttaatggca tccgtgataa aggtttaagc
     6841 gtgctcaact ggacggcgga agcggaacag ctacgcttgc gtttgttatg cgccgcaaag
     6901 tggttgccgg aatatgactg gccagcggtt gatgatgaaa gtttgttggc gacgctggaa
     6961 acgtggctgc tgccacatat gaccggcgta cattcactac gcggcctgaa atcactcgac
     7021 atttatcagg cactacgcgg attacttgat tggggaatgc agcaacgtct ggatagtgaa
     7081 ttgcctgcgc attacactgt gccgacggga agccggatcg ccattcgtta tcatgaagat
     7141 aacccgcccg cgctggcggt gagaatgcag gagatgtttg gtgaggccac taatccgacg
     7201 atcgcccagg ggcgcgtgcc gctggtgctg aagttgcttt cccctgccca aaggccgctg
     7261 caaatcacgc gtgatttggg ggccttctgg aaaggggcgt atcgtgaggt gcaaaaggag
     7321 atgaaagggc gttatcccaa acacgtctgg ccggacgacc cggcaaacac ggcaccgacg
     7381 cgacggacga aaaagtattc gtaaggttgg ttttctccct ctccctgtgg gagagggccg
     7441 gggtgagggc atcagcgcgc acgttcaccc tcaccctaac cctctccctc aagggagagg
     7501 ggaccgatcg agcgcaaatt ttgagagata tcttcttctg tcttgtaaca gaagaacaga
     7561 aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa tgaccgcgag
     7621 ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt
     7681 tacgaagatg acgatgatta cgacgattat gatgactatg aggatgaaga accgatgccg
     7741 cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg ctggctatgg
     7801 ctactgctaa aactggctat cgtttttgcc gtgctgatcg ccatttacgg cgtttatctc
     7861 gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc aactgcccgc ggcagtttat
     7921 ggccgaatgg tcaaccttga accagatatg accatcagca agaacgaaat ggtgaagctg
     7981 ctggaggcaa cacagtatcg tcaggtgtcg aaaatgaccc gtcctggcga atttaccgtg
     8041 caggccaaca gcattgagat gatccgccgt ccgtttgatt tcccggacag taaagaaggg
     8101 caggtgcgtg cgcgtctgac ctttgatggt gatcatctgg cgaccatcgt caatatggag
     8161 aacaaccgtc agttcggttt cttccgtctt gatccgcgtc tgatcaccat gatctcctcg
     8221 ccaaacggtg agcagcgttt gtttgtgccg cgcagtggtt tccctgattt gctggtggat
     8281 actttgctgg cgacagaaga ccgtcatttt tacgagcatg atggaatcag tctctactcc
     8341 attgggcgtg cggtgctggc aaacctgacc gcaggtcgca cggtgcaggg tgcgagtacg
     8401 ctgacgcaac agctggtgaa aaacctgttc ctctccagcg agcgttctta ctggcgtaaa
     8461 gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt acagcaaaga ccgtattctt
     8521 gagctgtata tgaacgaggt gtatctcggt cagagcggcg acaacgaaat ccgcggcttc
     8581 ccgctggcaa gcttgtatta ctttggtcgc ccggtagaag agctaagcct cgaccagcag
     8641 gcgctgttag tcggtatggt gaaaggggcg tccatctaca acccgtggcg taacccgaaa
     8701 ctggcgctgg agcgacgtaa tctggtgctg cgtctgctgc aacagcaaca gattattgat
     8761 caagaactct atgacatgtt gagtgcccgt ccgctggggg ttcagccgcg tggtggggtg
     8821 atctctcctc agccagcctt tatgcagctg gtgcgtcagg agttgcaggc aaaactgggc
     8881 gataaggtaa aagatctctc cggcgtgaag atcttcacta cctttgactc ggtggcccag
     8941 gacgcggcag aaaaagccgc cgtggaaggc attccggcac tgaagaaaca gcgtaagttg
     9001 agcgatcttg aaactgcgat tgtggtcgtc gaccgcttta gtggtgaagt tcgtgcgatg
     9061 gtcggaggtt ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc gcgtcgttcg
     9121 attggttccc ttgcaaaacc agcgacttat ctgacggctt taagccagcc gaaaatctat
     9181 cgcctgaaca cgtggattgc cgatgcgcca atcgcgctgc gtcagccgaa tggccaggtc
     9241 tggtcaccgc agaacgatga ccgtcgttat agcgaaagcg gcagagtgat gctggtggat
     9301 gcgttgacac gttcgatgaa cgtgccgacg gtaaatctgg ggatggcgct ggggctgcct
     9361 gcggttacgg agacctggat caaactgggc gtaccgaaag atcagttgca tccggttccg
     9421 gcaatgctgc tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc attccagacc
     9481 atcgccagcg gtggtaaccg tgcaccgctt tctgcactgc gttcggtaat cgcggaagat
     9541 ggtaaggtgc tgtatcagag cttcccgcag gcagaacgcg ctgttccggc gcaggcggcg
     9601 tatctgacac tatggaccat gcagcaggtg gtacaacgtg gtacgggtcg tcagcttggg
     9661 gcgaaatacc cgaacctgca tctggcaggg aaaacaggga ctaccaacaa taacgtagat
     9721 acctggtttg caggcattga cggcagcacg gtgaccatca cctgggtagg ccgtgataac
     9781 aaccagccga ccaaactgta tggagccagc ggggcaatgt cgatttatca gcgttatctg
     9841 gctaaccaga cgccaacgcc gctgaatctt gttccgccag aagatattgc agatatgggc
     9901 gtggactacg acggcaactt tgtttgcagc ggtggcatgc gtgtcttgcc ggtctggacc
     9961 agcgatccgc aatcgctgtg ccagcagagc gagatgcagc agcagccgtc aggcaatccg
    10021 tttgatcagt cttctcagcc gcagcaacag ccgcaacagc aacctgctca gcaagagcag
    10081 aaagacagcg acggtgtagc cggttggatc aaggatatgt ttggtagtaa ttaacatcta
    10141 agcgtgaaat accggatggc gagttgccat ccggtaaaat aacatcccat ctaagatatt
    10201 aaccctttct tttcatctgg ttgtttatta acccttcagg aacgctcaga ttgcgtaccg
    10261 cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt ataataatca ttctcgttta
    10321 cgttattatt cactttacat cagagatata ccaatggcgc gttccaaaac tgctcagcca
    10381 aaacactcac tgcgtaaaat cgcagttgta gtagccacag cggttagcgg catgtctgtt
    10441 tatgcacagg cagcggttga accgaaagaa gacactatca ccgttaccgc tgcacctgcg
    10501 ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg cgcggcagtc agctaccggc
    10561 actaaaaccg atacgccgat tcaaaaagtg ccacagtcta tttctgttgt gaccgccgaa
    10621 gagatggcgc tgcatcagcc gaagtcggta aaagaagcgc ttagctacac gccgggtgtc
    10681 tctgttggta cgcgtggcgc atccaacacc tatgaccacc tgatcattcg cggttttgcg
    10741 gcagaaggcc aaagccaaaa taactatctg aatggcctga agttgcaggg caacttctat
    10801 aacgatgcgg tcattgaccc gtatatgctg gaacgcgctg aaattatgcg tggcccggtt
    10861 tccgtgcttt acggtaaaag cagtcctggc ggtctgttga atatggtcag caagcgtccg
    10921 accaccgaac cgctgaaaga agttcagttt aaagccggta ctgacagcct gttccagact
    10981 ggttttgact ttagcgatgc gttggatgat gacggtgttt actcttatcg cctgaccggt
    11041 cttgcgcgtt ctgccaatgc ccagcagaaa gggtcagaag agcagcgtta tgctattgca
    11101 ccggcgttca cctggcgtcc ggatgataaa accaatttca ccttcctttc ttacttccag
    11161 aacgagccgg aaaccggtta ttacggctgg ttgccgaaag agggaaccgt tgagccgctg
    11221 ccgaacggta agcgtctgcc gacagacttt aatgaagggg cgaagaacaa cacctattct
    11281 cgtaatgaga agatggtcgg ctacagcttc gatcacgaat ttaacgacac ctttactgtg
    11341 cgtcagaacc tgcgctttgc tgaaaacaaa acctcgcaaa acagcgttta tggttacggc
    11401 gtctgctccg atccggcgaa tgcttacagc aaacagtgtg cggcattagc gccagcggat
    11461 aaaggccatt atctggcacg taaatacgtc gttgatgatg agaagctgca aaacttctcc
    11521 gttgataccc agttgcagag caagtttgcc actggcgata tcgaccacac cctgctgacc
    11581 ggtgtcgact ttatgcgtat gcgtaatgac atcaacgcct ggtttggtta cgacgactct
    11641 gtgccactgc tcaatctgta caatccggtg aatcccgatt tcgacttcaa tgccaaagat
    11701 ccggcaaact ccggccctta ccgcattctg aataaacaga aacaaacggg cgtttatgtt
    11761 caggatcagg cgcagtggga taaagtgctg gtcaccctag gcggtcgtta tgactgggca
    11821 gatcaagaat ctcttaaccg cgttgctggg acgaccgata aacgtgatga caaacagttt
    11881 acctggcgtg gtggtgttaa ctacctgttt gataatggtg taacacctta cttcagctat
    11941 agcgaatcgt ttgaaccttc ttcgcaagtt gggaaggatg gtaatatttt cgcaccgtct
    12001 aaaggtaagc agtatgaagt cggcgtgaaa tatgtaccgg aagatcgtcc gattgtagtt
    12061 actggtgccg tgtataatct cactaaaacc aacaacctga tggcggaccc tgagggttcc
    12121 ttcttctcgg ttgaaggtgg cgagatccgc gcacgtggcg tagaaatcga agcgaaagcg
    12181 gcgctgtcgg cgagtgttaa cgtagtcggt tcttatactt acaccgatgc ggaatacacc
    12241 accgatacta cctataaagg caatacgcct gcacaggtgc caaaacacat ggcttcgctg
    12301 tgggctgact ataccttctt tgacggtccg ctttcaggtc tgacgctggg caccggtggt
    12361 cgttatactg gctccagcta tggtgatccg gctaactcct ttaaagtggg aagttatacg
    12421 gtcgtggatg cgttagtgcg ttatgatctg gcgcgagtcg gcatggcggg ctccaacgtg
    12481 gcgctgcatg ttaacaacct gttcgatcgt gaatacgtcg ccagctgctt taacacttat
    12541 ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg caacctttcg tttctaattt
    12601 ctcttttggg gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca
    12661 cgaatcattc cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca
    12721 cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc
    12781 acaacggttc tggtaaatcc actctgctca aaatgcttgg tcgtcatcag cctccgtcag
    12841 aaggggagat tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgtttgccc
    12901 gcaaagtggc ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac
    12961 tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg gcgctttggt gcggcagatc
    13021 gcgaaaaggt cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg
    13081 tcgatagtct ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg
    13141 atagccgttg tctgttgctc gacgaaccga cttcagcgct ggatatcgcc caccaggttg
    13201 atgtgctggc gctggtgcac cgtttaagtc aggaacgtgg cctgacggtc attgccgtgt
    13261 tgcacgatat caatatggcg gcacgctact gtgattatct ggtcgccctg cgcagtggtg
    13321 aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg cgaaaccctc gaaatgattt
    13381 atggcatccc gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt
    13441 attgatgagc ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc
    13501 tccgttgtta tggcagatga ataccgccca cgcggcggct attgacccca atcgtattgt
    13561 ggcgctggag tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt
    13621 ggcggatacc atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat
    13681 tgacgtcggt ttgcgtacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt
    13741 tatggtctgg tcggcaggat atggcccttc atcagaaatg ctggcacgta ttgcgccggg
    13801 tcgcggattt aacttcagtg acggcaaaca tccgttggcg atggcgcgta aatcgctgac
    13861 ggaaatggca gatttactta acctgcaaag cgcagcggaa acgcatttag cgcaatacga
    13921 agactttatc cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttactgct
    13981 gacgacgctt atcgatccgc gccatatgct ggtcttcggt ccaaacagct tgttccagga
    14041 aattctcgat gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag
    14101 taccgccgtc agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga
    14161 tcacgacaac agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc
    14221 gtttgtccgt acagggcgct ttcagcgcgt acccgcagtc tggttttatg gtgcaacgct
    14281 ttcggcaatg cactttgtgc gtgttctgga taacgccatc ggaggtaaag cgtgagtaaa
    14341 cgaattgcgc ttttcccggc gttattgctg gcgctgttag tgattgtcgc tacggcgctc
    14401 acctggatga acttctcaca ggcgctgccg cgcagtcagt gggcgcaggc cgcctggtcg
    14461 ccggatattg acgtcatcga gcagatgatt tttcactaca gcttgttgcc gcgtctggcg
    14521 atttcgctgc tggtgggcgc gggcctgggg ctggtgggcg tgctgtttca gcaagtactg
    14581 cgtaacccgc tggcggaacc gacgacgttg ggcgttgcaa caggcgcgca actggggatt
    14641 accgtcacca cgctctgggc gatccctagc gcgatggcga gccagtttgc tgcgctggca
    14701 ggggcttgcg ttgttggctt aattgtcttt ggcgtcgcgt gggggaaacg gctgtcgccg
    14761 gtaacgctga ttttcgcggg gttggtagtg agcctttatt gcggcgcaat caatcagtta
    14821 ctggttatct tccatcatga ccagttgcaa agcatgtttc tgtggagcac tggaacgctg
    14881 acgcaaaccg actggggcgg tgttgagcgt ttatggccgc agttgctggg cggtgtgatg
    14941 ctgacgttgc tgctacttcg tccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc
    15001 aatctcgggc tggccttgtc gcttgcgcgt ctggcggcgc tgtcgctggc gattgtcatc
    15061 agtgcgctgc tggtgaacgc tgtggggatt atcggcttta tcgggttgtt cgcgccgctg
    15121 ctggcaaaaa tgctgggggc gcggcgtctg ctgccacgac tgatgctggc gtcgttgatt
    15181 ggtgcgctga tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg
    15241 gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgctg
    15301 ccgcgtttac gcagcattag cgcgccggat atgaaggtta acgatcgtgt cgcgactgaa
    15361 cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc tgttgttgat ggctgtggtg
    15421 gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt gggcgagcgg ggcgttgctc
    15481 gaggatttaa tgccctggcg ctggccgcga attatggcgg cgctgtttgc gggcgtcatg
    15541 ctggcggtag cgggctgtat tattcagcga ctgaccggca acccgatggc aagcccggaa
    15601 gtgctgggga ttagctccgg cgcggcgttt ggtgtggtgt taatgctgtt tctggtaccg
    15661 ggtaatgcct ttggctggtt gttacccgca ggcagtctcg gggcggcggt gacgctgttg
    15721 atcattatga tcgccgccgg acgcggtgga ttttccccac accgcatgtt actggcgggg
    15781 atggcgttaa gtaccgcgtt caccatgctt ttgatgatgt tgcaggcaag tggtgacccg
    15841 cgaatggcgc aagtgctgac ctggatttcc ggttcgacct acaacgcgac cgatgctcag
    15901 gtctggcgca ccggaattgt gatggtgatt ttgctggcga ttactccgct gtgccgccgc
    15961 tggctgacca ttttaccgct gggtggtgat accgcgcggg cggtagggat ggcgctgacg
    16021 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacgatgact
    16081 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt
    16141 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtgttc
    16201 gctgactggt gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg
    16261 acctttatcg gtgcgccata ttttatctat ttgctgagaa agcagagccg ttaatagaat
    16321 gcctcgcccg gtgggcgagg caaaattatc acaacttcgc gaacacccga cgtgcagcat
    16381 cgatggtgtt attgatatct tccatgctgt gcgccacaga cataaagccc gcttcaaacg
    16441 ctgacggtgc caggtaaaca ccttcatcca gcatcatatg gaagaaacgc ttaaaacgct
    16501 cgacgtcaca ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaga
    16561 taccgaacat accaccgacg tggttaacga ccagcggaat tcccgcttct tctgccgctt
    16621 ccagcagacc ttctgccaga cgtgttgtca gctcatccag cgtttcgtga atgcccggct
    16681 gcgcgacttc attcagacag gcgaaacccg ctgccatcgc aatcgggtta ccggaaagcg
    16741 tacccgcctg atagaccgga cctgtcgggg ccagcgcatc cattacatca cgacgaccac
    16801 cgaatgcgcc taccggcatt ccaccgccga tgatttttcc gaggcaggtt aaatctggca
    16861 ctacggcgaa ataatcctgt gcgccagcta gcgctacgcg gaaaccggtc atcacttcat
    16921 cgatgatcag caacgcgcca aattcgtcgc acagcgcacg cagacctggc aggaactctg
    16981 gcagcggcgg aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct
    17041 cttgcgggta ttgctcaaac gcggcgcgta cagaggccag atcgttataa gtacaggtta
    17101 aggtatgttt ggcgaaatct gccggaacgc ccggcgagtt tggctggcct aacgtgagtg
    17161 cgccagaacc ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt
    17221 taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atcgtcgcct
    17281 cggtgccgga gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt
    17341 gcgccatttt cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt
    17401 caatcacggc attgcggatc gccggatggt tatggcccag caccatcggc ccccaggaac
    17461 cgacataatc gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt
    17521 cgataaacag tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc
    17581 cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct
    17641 gattcgtaga aaaagtgaat ggctgctatt ccatgttatt cataacaagt taaatactcg
    17701 tcaaacatca ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt
    17761 gcatttgaaa cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc
    17821 atcttatttg tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa
    17881 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat
    17941 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggct
    18001 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta
    18061 catactgctg ataattatcc gctactgtta accgtcgctt ttctctgttc ggcggtgctg
    18121 gcgatgttcg gctacttttt ggtgcgcaaa tacgcgcccg aagcaggggg atcggggatc
    18181 ccggaaattg aaggggcgct ggaagatcaa cgtcctgttc gctggtggcg tgtattgccg
    18241 gtgaaattct ttggcgggct ggggacactc ggcggaggca tggggctagg gcgcgaagga
    18301 ccaaccgtgc agatcggcgg caatatcagt cgcatggtgc ttgatatttt ccgcctgaaa
    18361 ggtgatgaag ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc
    18421 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc
    18481 tatacgttaa tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac
    18541 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt
    18601 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt
    18661 aataaatggg tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc
    18721 aaatgggtac tgatgggggg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca
    18781 ccagcaacgt cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc
    18841 atgggaatgc tggtgtttat cttcgtcgct cgggtcatta ccaccttact ctgcttctct
    18901 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc
    18961 gctttcggaa tggttgccgt tgagctgttt ccgcaatatc acctggaggc ggggacgttt
    19021 gctattgccg gaatgggggc attactggcg gcatctattc gcgcgccgtt aacggggatc
    19081 attctggttc tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt
    19141 cttggcgcaa cactattagc gcaatttacc ggcgggaaac ctctatactc ggcgattctt
    19201 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
    19261 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
    19321 aatttgcccg attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
    19381 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
    19441 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
    19501 ttcacctttg atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc
    19561 ctggtggttg atccgatgag cctgcaatat ctggtcggcg gttccgttga ctacaccgaa
    19621 ggactggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc
    19681 ggttcttcct ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct
    19741 tctctcttca acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
    19801 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
    19861 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg
    19921 cgtagatttc tgtacgtaaa atcatgggga tttcgcggac cagtacatca cgaatgatcc
    19981 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
    20041 taaaggcttt attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg
    20101 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
    20161 tgaccatggc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
    20221 gcgccatgtc gcgaattgtt ccgtcgccta ctgcggtaac cacgcccagt accagaacac
    20281 caaaagggtc catacgcaat tttccggcta acaaaacgcc gtagatggca aataccgctg
    20341 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag
    20401 gtcaggcact aatctacctg tgaaagcgca ttacagagct gttgtgctgc gaggataata
    20461 cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt
    20521 tcaccccagt attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatagcc
    20581 tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct
    20641 ttaaagatgt tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca
    20701 ctggtaaatg gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca
    20761 tattgcgctt tcaattgcac gtactgatcc agcagggatt gcgcggcttg ttcggccttg
    20821 tctggttgcg gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt
    20881 gtcgcatcga cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg
    20941 gcattacctc cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga
    21001 ttcatcccct gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa
    21061 tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga
    21121 gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg gcgcgagaaa agacagggcg
    21181 accagcgccc tgaacagtga tttagccatg tgccagtttc tgcaccagtg actcaaccat
    21241 caggctggac tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg
    21301 atcggccacg tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac
    21361 atgggcgatt gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat
    21421 tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac
    21481 agcgttaaga ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc
    21541 tttaaagcct gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatc
    21601 cgcgtcgtga taacgcgctt cgtccgagac aacgatatcg cccactttca gcgttggtgc
    21661 caggccaccg gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcag
    21721 agtggcaccc agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt
    21781 tccattcagt tggccggtat agatttcgca accgccgaga ctgatagttt gacggttttc
    21841 gattttgtca cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat
    21901 agatttactc gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc
    21961 gtatttctca cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc
    22021 atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg
    22081 agagcgatcg cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc
    22141 aggtttttcc actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag
    22201 tccagcaggt gggacgctac atcgccaaag aaattttaag ccgtctgaaa gagcttaaat
    22261 tgctggaagc atacggcctg gatgaactga ccggcccctt tgaaagcatt gttgagatgt
    22321 catgcctgat gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcagcgataa
    22381 atgactggtt tcgccaacgt ttgcacccgg aagatgccga aagccagcct ctgactgacg
    22441 atcgctgcag cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc
    22501 ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg
    22561 tgcatacatt gatgcggatg aatctcactt gggcacaggt tggcggtatt ttaaaatata
    22621 cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc
    22681 cgggttatta tctttctgaa gaagcctata ttgcccgctt gcgtaaagaa cttaatttgg
    22741 cgctttacag tcgttttcca ttaacgtgga ttatggaagc tgccgacgac atctcctatt
    22801 gtgtggcaga ccttgaagat gcggtagaga aaagaatatt taccgttgag cagctttatc
    22861 atcatttgca cgaagcgtgg ggccagcatg agaaaggttc gctcttttcg ctggtggttg
    22921 aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt
    22981 ttatgtattt acgggtaaac accctaaata aactggtacc ctacgcggca caacgattta
    23041 ttgataatct gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca
    23101 gcgaatgcag cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc
    23161 atccggatgt cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga
    23221 tttatcgtcc tttattaagc ctgtcgttat cagactttac tgaactggta gaaaaagaac
    23281 gggtgaaacg tttccctatt gagtcgcgct tattccacaa actctcgacg cgccatcggc
    23341 tggcctatgt cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg
    23401 aatattatta ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg
    23461 cgtgggatga ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg
    23521 aacaataaat ttttaccttt tgcagaaact ttagttcgga acttcgggct ataaaacgaa
    23581 tctgaagaac acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa
    23641 ccacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg
    23701 caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga
    23761 tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta
    23821 atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc
    23881 aggaaggttc tccgttccag agctctccgt tctgccaggg gggccagggc ggtaatggcg
    23941 gcggccagca acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag
    24001 gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccagctga
    24061 gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc
    24121 tgatccaaat ccagaacccg aaaaaactga ccgcaattaa gatggcggat tctgatgcac
    24181 tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt tggtctgggc gagacggtaa
    24241 cttccgggat tgtctctgcg ctggggcgta gcggcctgaa tgctgaaaac tacgaaaact
    24301 tcatccagac cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg gttaacctga
    24361 acggcgaact gatcggtatc aacaccgcga tcctcgcacc ggacggcggc aacatcggta
    24421 tcggttttgc tatcccgagt aacatggtga aaaacctgac ctcgcagatg gtggaatacg
    24481 gccaggtgaa acgcggtgag ctgggtatta tggggactga gctgaattcc gaactggcga
    24541 aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg cctaattcct
    24601 ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccga
    24661 tcagcagctt tgccgcactg cgtgctcagg tgggtactat gccggtgggc agcaaactga
    24721 ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg cagcagagca
    24781 gccagaatca ggttgattcc agctccatct tcaacggcat tgaaggcgct gagatgagca
    24841 acaaaggcaa agatcagggc gtggtagtga acaacgtgaa gacgggcact ccggctgcgc
    24901 agatcggcct gaagaaaggt gatgtgatta ttggtgcgaa ccagcaggca gtgaaaaaca
    24961 tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc
    25021 gcggcgacag caccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaatac
    25081 gggaaggggt tctccttaca atctgtgaac ttcgccacaa ctccatacat cttcatcatc
    25141 ctttgggcat ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataac
    25201 ggaggactta tggctggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt
    25261 accatgcgca tcatcgatac caatatcaac gtaatggatg cccgcgggcg aattatcggc
    25321 agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag
    25381 ggacgagtcg tcgatatcga tgacgcggta gcacgtcatc tgcacggtgt gcggcagggg
    25441 attaatctac cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa
    25501 ccagagaatc tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa
    25561 cagtcgcggt tgatgcactt gttggcgcag gatagccgtt tgcgggaaga actggtgatg
    25621 aacctgattc aggcagagga gaatactccc gcacttctga atgggcgcaa cggctgggga
    25681 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg
    25741 tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta
    25801 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    25861 cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
    25921 tgaaagagta cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg
    25981 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
    26041 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    26101 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    26161 acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc
    26221 tggcaacgtc aaaggcgttg tttattcatc gtaataccct ggagtatcgg cttaatcgta
    26281 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
    26341 cgttacaact ggatgaagag cggtagatta tgcgttaagg ttgaggcagc gctggctcat
    26401 tcccctcagc ccgaagggga gaggggaatg cccgagttaa ccgtcaattc gggattattt
    26461 acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc
    26521 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
    26581 acagatttga tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc
    26641 tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa
    26701 cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
    26761 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
    26821 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
    26881 tagcatttgg cttggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
    26941 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggcgat attccatggg
    27001 atggattagc acatttatct tcgttttgcg aattacatga cagtccgaaa caagatgaaa
    27061 aagtatttaa aaaaggggga ggaagaatga gtaggacgaa ttattctgag ccagaaagca
    27121 tacgggcagc atcatgctgc ccgtatatgt actcttaatc gatggtacgc agcagttcgt
    27181 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
    27241 ggctgtattt gccatctttt gacggcagat tacctgaaac aaccacagac cccgccggaa
    27301 cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
    27361 atacgcccat agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcagagc
    27421 gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca
    27481 cgccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac
    27541 cgacggtcgc ccaggtatca accatggtgc cttcatcaac atatgcgccg atgttaacgt
    27601 aagacggcat cagcacggtg ttacgggcaa taaacgcacc ctgacgtacc gccgctggtg
    27661 gcacaacgcg gaagccttct ttctggaaac gtgcttcgtc gtagtcggcg aatttcatcg
    27721 gcactttgtc gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga
    27781 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
    27841 ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
    27901 taacggtgtc tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
    27961 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
    28021 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
    28081 gcgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
    28141 taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg actcgcgcca
    28201 gcagtccagg ttggtcgagg gcgatcagtt cgaggaacga tttgcggtcg gtatgggtcg
    28261 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg
    28321 gctgcggtgg ctgccagcta ctttgcgtca gcacctgctc cagaccaaac cgaataacct
    28381 catggcgatc tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg
    28441 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta
    28501 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
    28561 tgcctccacg cgtagcctgc gggctaagca ataccagcgg tttgcttaaa tcatgctgta
    28621 ataaatggcg ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
    28681 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
    28741 ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
    28801 cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc
    28861 tattccacag tgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
    28921 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa
    28981 tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca
    29041 gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac
    29101 cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
    29161 aaatcagctc agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc
    29221 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    29281 ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca
    29341 tatacgcgcc gagcacgcta tggcgatgca ttggcaatag tccgcgccgc accgctccgg
    29401 ggtgacgcag aatgctcaaa aacagttttc gtgcttgcgg aatattacac agcggttgtt
    29461 gcagatggcg acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg
    29521 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    29581 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgagtggac
    29641 gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct
    29701 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    29761 gttcgttgcc ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata
    29821 acagacgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta
    29881 tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccgacca
    29941 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtgaatat
    30001 cgcgcaagcc gccagggcta cttttgatgt ctggttcaag gttgtagctg gtgccatggt
    30061 aacgctgatg gcgctggttc tgttcttcaa ctttcgctgc gtagaacttg tcggaaggcc
    30121 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta
    30181 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccaacatgc
    30241 actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta
    30301 acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta
    30361 aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcaaccaatg
    30421 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcta
    30481 tgaactcggt acgtgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac
    30541 ccagccaacg ctggaaagta tcgatatggg cttttatccc acagacggtt aattcatcac
    30601 ggggccaggc gcatggattt tgcggttgac cgggcagggt ggagagagcg gtgtttgcgt
    30661 actgttctgg aagggtattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa
    30721 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    30781 tccttgcgta gcgtcaaaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat
    30841 tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    30901 tcttttttac ccgcgttgac cattggctca atggtgaacg tcatccccgg tttcagcacg
    30961 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    31021 ataccgtgtc cgcaatattc acgaacgacg gagaagcctt ctgcttcgac aaatttctga
    31081 atcgccgcac cgatttcgcg cagattaatg cctggtttta ccatgcgtag cgccaggtac
    31141 aggctttctt gcgtgatgcg gcacaggcgt tcgcccatga tggtcggctt accgacgata
    31201 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    31261 atatcgccat ctttcagcag cttagcatcg tccgggatac cgtggcacac cacttcatta
    31321 atagagatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    31381 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    31441 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    31501 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    31561 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    31621 tcattctgaa tttcgccaaa cgtgccactg aaggttttct ataatagaaa attcgacgtc
    31681 tgatgctgta cacagcgcca acaattattg gtgtccacga cgtatttgtg gtataaagcg
    31741 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    31801 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    31861 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    31921 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    31981 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    32041 actgtaccga tgttcaacga agctctggct gaactgaaca agattgtttc tcgcaaaggt
    32101 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    32161 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    32221 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    32281 ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    32341 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    32401 gctgaccacg aacacattgc tatcaaagaa gcaaacaact tgggtattcc ggtatttgct
    32461 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    32521 gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt
    32581 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    32641 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    32701 cttacatcag taagtgaccg ggatgagcga gcgaagacaa cgcatctgcg gcgcgaaata
    32761 tgaaggggga gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    32821 gccccctttt tcacttttat atctgtgtgg tttaatgccg ggcagatcac atctccgagg
    32881 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    32941 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    33001 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    33061 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    33121 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    33181 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
    33241 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
    33301 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
    33361 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    33421 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    33481 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    33541 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    33601 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    33661 gtttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    33721 cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct gagggcggct tctttttgtg
    33781 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    33841 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    33901 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    33961 aggcttcggt attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt
    34021 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    34081 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    34141 aatgaacggc ttggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    34201 gtccgctatt ccattgaatg gcgtgtgcga tagctacagc tgggcagaag ctatcagcct
    34261 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    34321 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    34381 caaagttgac ggcgtgttta ccgctgatcc ggcgaaagat tcaaccgcaa ccatgtacga
    34441 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    34501 cacgctggct cgtgaccata aattaccgat tcgtgttttc aatatgaaca aaccgggtgc
    34561 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    34621 atggataaat aagggtaaga ttccgcgtaa gtatcgcggg ggcgtaagtc tggttataag
    34681 gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca gcaaccggag cgtacaaaag
    34741 tacgtgagga tggcgagcac tgctcggggc caaaatggca aataaaatag cctaataatc
    34801 cagacgatta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    34861 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    34921 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    34981 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    35041 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    35101 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcctccga
    35161 tctcggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    35221 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    35281 tgcagtacgt aatgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    35341 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    35401 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    35461 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    35521 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    35581 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    35641 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    35701 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    35761 agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    35821 cgcttgagga ggttgatcag gtgatggcag ccattgttgg tgctgctggg ctgttaccta
    35881 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    35941 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    36001 atagcgaaca taacgctatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    36061 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    36121 tccgtgagac gccattgcgc gatttggcaa caatgacgcc cgatcaagct tgccgtcatc
    36181 cgaactggtc gatgggacgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc
    36241 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    36301 ttcacccgca gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc
    36361 agctggggga accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg
    36421 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    36481 cggattatga gcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggccagg
    36541 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac
    36601 aaatccgctt tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc
    36661 gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc gaacgcgagt gaagtcgcca
    36721 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    36781 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    36841 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    36901 agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact
    36961 tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg
    37021 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc
    37081 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    37141 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    37201 tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    37261 tattggcgat accagtcgtt ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    37321 gctaacagcc ggaaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    37381 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    37441 agatcagata gatgaagaga tgttaaacca gcatgtctgt atgcatgaac tggcccctgt
    37501 agatttagta attaggactg ggggagagca tcgcattagt aactttttgc tttggcaaat
    37561 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    37621 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    37681 tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt
    37741 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt
    37801 aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    37861 tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt
    37921 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    37981 ttcgttgggt tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc
    38041 aatctggcgt aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt
    38101 cttctggggc atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc
    38161 aatatggctg ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt
    38221 tggcaaattg tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca
    38281 aggctttata ggtggactcg ctactgcagc ggtaatctca tggggttatg gcatgtgggc
    38341 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    38401 agtgctcggc gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg
    38461 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    38521 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    38581 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    38641 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    38701 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    38761 gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    38821 ccggaactcc gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt
    38881 gccgcaggtc cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt
    38941 attggtgtgc ctggcgtacg tccggtggtt ggcgaaatag cagccaattc gatagctgcg
    39001 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    39061 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca
    39121 gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    39181 tttgagcctg ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa
    39241 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    39301 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    39361 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt
    39421 cccttgtctt tgacattaat cccggggagt aaaccgggta atggtaaagc gattggtttt
    39481 gtcggtattg agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat
    39541 gggccgttca acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg
    39601 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    39661 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    39721 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    39781 gacggggggc atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag
    39841 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    39901 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    39961 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    40021 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    40081 cggccctcct cagtatgccg gtgcgtacag gcgacacggt taatgatgaa gatatcagta
    40141 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    40201 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    40261 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    40321 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    40381 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    40441 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    40501 acattgttgg taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg
    40561 acgaagtgcc gtggtggaat gtggtaggcg atcgtaaata ccagaaacag aaactggcgg
    40621 gcgatcttga aaccctgcgc agctactatc tggaccgcgg ttatgcccgt ttcaacatcg
    40681 actctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca
    40741 ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact
    40801 ccgctgaaat tgagcagctg actaagatcg agccgggcga gctgtataac ggcactaaag
    40861 tgaccaaaat ggaagatgac attaaaaagc ttctcggtcg ctatggttat gcctatccgc
    40921 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    40981 ttgatgtggg taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga
    41041 aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggttg gggagcgatc
    41101 tggtcgatca gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg
    41161 atacccaacg tgttccgggt agcccggacc aggttgatgt agtctacaag gtgaaagaac
    41221 gtaacactgg tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc
    41281 aggctggcgt gcaacaggat aactggttag gtacaggtta tgctgttggt atcaacggga
    41341 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag
    41401 atggcgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    41461 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgattaacg
    41521 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    41581 ctcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    41641 aggataacag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    41701 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    41761 ttcctggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    41821 tcgatgacga tcacaaatgg gttgttctgg gtcgtacccg ctggggttat ggtgatggtt
    41881 taggcggcaa agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtgc
    41941 gtggcttcca gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta
    42001 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta
    42061 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    42121 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttcattc ttctgggata
    42181 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    42241 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    42301 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    42361 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    42421 gtgtagcgat gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc
    42481 ctccggtgca aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt
    42541 cttggtttag cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg
    42601 ggcagcctgt tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag
    42661 ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa
    42721 aaactgcagt ccatgaaagc gggcagcgat cgcaccaaac tggaaaaaga cgtgatggct
    42781 cagcgccaga cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc
    42841 aacgaagaac gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac
    42901 agccaggata tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta
    42961 aaagacatca ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg
    43021 gctgatttag cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc
    43081 ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa
    43141 taccgtgagc atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt
    43201 cctttcgcga aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg
    43261 gcgcaaattt tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc
    43321 gacgcgacgg cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc
    43381 ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc
    43441 aaaatcggtg caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc
    43501 ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc
    43561 aacgatcgtg gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc
    43621 gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc tggatgacac tgttattggc
    43681 aatggcgtga tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat
    43741 acggcggttg ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg
    43801 atcggcggag ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg
    43861 ggcatgggta tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg
    43921 ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg
    43981 agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt
    44041 tgttcgccaa actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt
    44101 gtcgtttctt atattttgac aggaagagta tcttgactac taacactcat actctgcaga
    44161 ttgaagagat tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc
    44221 tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat
    44281 tcttccaggg ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa
    44341 tggcacaggc aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc
    44401 tgtactactt cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc
    44461 aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg
    44521 ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg
    44581 aggcctgata cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg
    44641 gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg tatcgttgga ccccatgtcg
    44701 aaattggtga gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg
    44761 gccgcgataa tgagatttat cagtttgcct ccatcggcga agttaaccag gatttgaaat
    44821 atgctggcga accgacccgt gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca
    44881 ccattcatcg tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac
    44941 tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca
    45001 acaacgcaac gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga
    45061 ccgcagtcca tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg
    45121 tggcgcagga cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg
    45181 tcaatatcga agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca
    45241 atgcgtataa gctaatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg
    45301 ctgaactggc ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa
    45361 cgcgcggtct gattcgttaa tgactgacca gcgtccatta acgattgccc tggtcgccgg
    45421 agaaacctcc ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc
    45481 caacgcccgc tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg
    45541 gtacgaaatg gaagaactgg cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg
    45601 cttactgcat attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt
    45661 tgttggtatt gatgcgcccg acttcaatat tactcttgaa ggtaacctca aaaagcaggg
    45721 tatcaaaacc attcattacg tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt
    45781 caaaataggc agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta
    45841 tgacaaatac aatgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccgtt
    45901 agatccagat aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct
    45961 ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcttgaa
    46021 aactgcccag cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa
    46081 tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa gtcgcgccag acctgtcggt
    46141 tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc agcgatgcgg cactactggc
    46201 gtcggggacg gcagccctgg agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg
    46261 catgaagcct tttaccttct ggttggcgaa gcggctggtg aaaactgatt atgtctcgct
    46321 gccaaatctg ctggcgggca gagagttagt caaagagtta ttgcaggaag agtgtgagcc
    46381 gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccacgcgat
    46441 gcacgatacc ttccgtgaac tgcatcagca gatccgctgc aatgccgatg agcaggcggc
    46501 acaagccgtt ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt
    46561 gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg
    46621 atccttgacc cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa
    46681 cgccgtctgg cgctctatga agagatcaaa gagaaagcgt tgagctggag tctgggccgc
    46741 gcggaacccc acgaaatcga cgagctgaac attcttcatg cgaccatgct ggcgatgcag
    46801 cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc
    46861 ccgaaattac cgatgcctgc gatggctgtg gtgaaagggg atagccgcgt accagaaatc
    46921 agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt
    46981 gttttcccgc aatatggttt tgcccaacat aaagggtatc caaccgcttt tcatctggaa
    47041 aaactggctg aatatggcgc gactgaacat catcgtcgca gctttgggcc tgtcaaacgc
    47101 gcactgggac ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg
    47161 tctgaaccac gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcttg
    47221 gccaaaaccg caccgctggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc
    47281 accgatttca ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg
    47341 attaagccta tcgtcggggc agattttaat gtccagtgcg acctgctggg tgatgagtta
    47401 acccacctga cggtactggc ggcgaacaat accggctatc agaatctggc gttgctgatc
    47461 tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt
    47521 atcgaattaa acgaagggtt gatccttctt tccggcgggc gcatgggcga cgtcggacgc
    47581 agtcttttgc gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac
    47641 ttcccggatc gctattttct cgagctgatc cgcaccggca ggccggatga agaaagctat
    47701 ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc ccgtcgtggc gactaacgat
    47761 gtgcgcttta tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac
    47821 ggctttaccc tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt
    47881 agcgaagagg agatgtgtga gctgtttgcc gacatcccag aagcccttgc caacaccgtt
    47941 gagatcgcca aacgctgtaa cgtaactatg cgtcttggtg aatacttcct gccgcagttc
    48001 ccgaccgggg acatgagcac cgaagattat ctggtcaagc gtgcaaaaga aggcctggaa
    48061 gagcgtctgg catttttatt ccctgacgag gaagaacgtc ttaaacgccg cccggaatat
    48121 gacgaacgtc tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc
    48181 ctcatcgtta tggaatttat ccagtggtcg aaagataacg gcgtaccggt agggccaggc
    48241 cgtggctccg gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg
    48301 ctggaatttg acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac
    48361 ttcgacgttg acttctgtat ggagaaacgc gatcaggtta tcgaacacgt ggcggacatg
    48421 tacggtcgtg atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg
    48481 atccgcgacg taggccgcgt gctggggcat ccgtacggct ttgtcgaccg tatctcgaaa
    48541 ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg
    48601 ccggaaatct acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg
    48661 gaaggggtca cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa
    48721 attaccgatt ttgcgccgct ttattgcgat gaagagggca aacatccggt cacccagttt
    48781 gataaaagcg acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg
    48841 ctcaccatca tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag
    48901 ccaccgctgg atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa
    48961 cgctcggaaa ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag
    49021 cgtctgcaac ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccctggt
    49081 ccgttgcagt cagggatggt ggataacttt atcgaccgta aacatggtcg tgaagagatc
    49141 tcctatccgg acgtgcagtg gcagcatgaa agcctgaaat cggtactgga gccaacctac
    49201 ggcattatct tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc
    49261 ctcggtggcg cggatatgct gcgccgtgcg atgggtaaga aaaagccgga agagatggct
    49321 aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg
    49381 atgaaaatct tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct
    49441 gcggcctatg ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag
    49501 tttatggcgg cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg
    49561 gatgagtgct ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac
    49621 catttccacg tcaacgacga cggcgaaatc gtgtatggta ttggcgccat caaaggggta
    49681 ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa
    49741 ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa
    49801 ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg
    49861 ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc
    49921 gatatgttcg gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc
    49981 caaccgtggc cggagcaggt ggtattagat ggggaacgtg aaacgttagg cctgtacctg
    50041 accggacacc ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg
    50101 ctgaaagaca tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt
    50161 gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac
    50221 cgttccgggc ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg
    50281 ctggaaaaag accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt
    50341 gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct
    50401 cgcgggcttg ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc
    50461 cgtcagtctc tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg
    50521 gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt
    50581 ttattaaacg atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa
    50641 tacaggaata ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa
    50701 gcgaaaatcg attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc
    50761 gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc
    50821 gatctcggtg catggcagat tgcgcaactg gcacgccatc cgcagcgtcc ttataccctg
    50881 gattacgttc gcctggcatt tgatgaattt gacgaactgg ctggcgaccg tgcgtatgca
    50941 gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt
    51001 ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg
    51061 ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg ctgaacgctt taagatgcct
    51121 atcatcacct ttatcgacac cccgggggct tatcctggcg tgggcgcaga agagcgtggt
    51181 cagtctgaag ccattgcacg caacctgcgt gaaatgtctc gccttggcgt accggtagtt
    51241 tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg cgattggcgt gggcgataaa
    51301 gtgaatatgc tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt
    51361 ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg
    51421 cgtctgaaag agctgaaact gatcgactcc atcatcccgg aaccgctggg cggcgctcac
    51481 cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac tgctggcgga tctggccgat
    51541 ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac
    51601 ggttacgcgt aattcgcaaa agttctgaaa aagggtcact tcggtggccc ttttttatcg
    51661 ccacggtttg agcaggctat gattaaggaa ggattttcca ggaggaacac atgaacatca
    51721 ttgccattat gggaccgcat ggcgtctttt ataaagatga gcccatcaaa gaactggagt
    51781 cggcgctggt ggcgcaaggc tttcagatta tctggccaca aaacagcgtt gatttgctga
    51841 aatttatcga acataaccct cgaatttgcg gcgtgatttt tgactgggat gagtacagtc
    51901 tcgatttatg cagcgatatc aatcaactta atgaatatct cccgctttat gccttcatca
    51961 acacccactc gacgatggat gtcagcgtgc aggatatgcg gatggcgctc tggttttttg
    52021 aatatgcgct ggggcaggcg gaagatatcg ccattcgtat gcgtcagtac accaacgaat
    52081 atcttgataa cattacgccg ccgttcacga aagccttgtt tacctacgtc aaagagcgga
    52141 agtacacctt ttgtacgccg gggcatatgg gcggcaccgc atatcaaaaa agcccggttg
    52201 gctgtctgtt ttatgatttt ttcggcggga atacccttaa ggctgatgtc tctatttcgg
    52261 tcaccgagct tggttcgttg cttgaccaca ccgggccaca tctggaagcg gaagagtaca
    52321 tcgcgcggac ttttggcgcg gaacagagtt atatcgttac caacggaaca tcgacgtcga
    52381 acaaaattgt gggtatgtac gccgcgccat ccggcagtac gctgttgatc gaccgcaact
    52441 gccataaatc actggcgcat ctgttgatga tgaacgatgt agtgccagtc tggctgaaac
    52501 cgacgcgtaa tgcgttgggg attctggggg ggatcccgcg ccgtgaattt actcgcgaca
    52561 gcatcgaaga gaaagtcgct gccactacgc aagcacaatg gccggttcat gcggtgatca
    52621 ccaactccac ctatgatggt ttgctctaca acaccgactg gatcaaacag acgctggatg
    52681 tcccgtcgat ccacttcgat tccgcctggg tgccgtatac tcattttcat ccaatctacc
    52741 agggtaaaag tggtatgagc ggcgagcgtg ttgcgggaaa agttttcttc gaaacgcaat
    52801 cgacccacaa aatgctggcg gcgttatcgc aggcttcact gatccacatt aaaggcgagt
    52861 atgacgaaga ggcgtttaac gaagccttta tgatgcatac caccacctcg cccagttatc
    52921 ccattgttgc ttcggttgag acggcggcgg cgatgctgcg tggtaatccg ggcaaacggc
    52981 tgattaaccg ttcggtggag cgggcgctgc attttcgcaa agaggtccag cggctgcggg
    53041 aagagtctga cggttggttt ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat
    53101 gcaggcccgt tgcgcctggc gaacaatggc acggctttaa cgatgcggat gccgatcata
    53161 tgtttctcga tccggttaaa gtcactattt tgacaccggg gatggacgaa cagggcaata
    53221 tgagtgagga ggggatcccg gcggcgctgg tagcaaaatt cctcgacgaa cgtgggatcg
    53281 tagtagagaa aaccggccct tataacctgc tgtttctctt tagtattggc atcgataaaa
    53341 ccaaagcaat gggattattg cgtgggttga cggaatttaa gcgctcttac gatctcaacc
    53401 tgcggatcaa aaatatgctg cccgatctct atgctgaaga tcccgatttc taccacaata
    53461 tgcgtattca ggatctggcg caagggatcc ataagctgat tcgtaaacac gatctttccg
    53521 gtttgatgtt gcgggcattc gatactttgc cggaaatgat catgacgcca catcaggcat
    53581 ggcagagaca gattaaaggt gaagtagaaa ccattgcgct ggaacaactg gtcggtagag
    53641 tatcggcaaa tatgatcctg ccttatccac cgggcgtacc gctgttgatg ccaggagaaa
    53701 tgctgaccaa agagagccgc acggtactcg attttctact gatgctttgt tccgtagggc
    53761 aacattaccc cggttttgaa acggatattc acggcgcgaa acaggacgaa gacggcgttt
    53821 accgcgtacg agtcctaaaa atggcgggat aacttgccag agcggcttcc gggcgagtaa
    53881 cgtgctgtta acaaataaag gagacgttat gctgggttta aaacaggttc accatattgc
    53941 gattattgcg acggattatg cggtgagcaa agctttctac tgcgatattc ttggtttcac
    54001 gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa
    54061 tgggcaatat gtgattgagc ttttctcttt tccgttcccg ccggagcgcc ccagccgacc
    54121 ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat gacatcgatg cggcagtggc
    54181 gcaccttgaa agccataacg tgaagtgtga agccatccgt gtcgatccat acacgcaaaa
    54241 acgcttcacc ttctttaacg atccggacgg gctgccgttg gaactgtatg agcagtaagg
    54301 cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt gctactgtaa aatcgcacca
    54361 tcatgacact cacgctcaat agacaacttc tcacctcacg ccagattctg gtggccttta
    54421 gcggcgggct tgactccacc gttctgctgc atcagttggt gcagtggcgg acggaaaatc
    54481 cgggcgtcac tctgcgcgct attcatgtgc atcacggttt aagtgccaat gccgatgcct
    54541 gggttacgca ttgcgaaaac gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg
    54601 tacaacttgc gcaagagggg ctggggattg aggcccaggc gcggcaggca cgttatcagg
    54661 catttgcccg taccctattg cccggcgaag tgctggtcac cgcgcaacat ctcgacgatc
    54721 aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta
    54781 tggcggaagt ttcggagttt gctggaacac ggcttatccg cccgttgctt gcccgcacgc
    54841 ggggggaact ggagcagtgg gcattggcgc atggtttacg ctggattgaa gacgaaagta
    54901 atcaggacga ctcatacgat cgtaactttc tgcgcctgcg cgtagtgccg ttattgcagc
    54961 agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc cacactttgt gctgaacaag
    55021 agagtctgtt ggatgaactg ctggcggatg atttagcaca ctgccaaacg tcgcagggga
    55081 cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg tcgcgcggcg attattcgcc
    55141 gctggctggc agggcagaat gcaccgatgc cttcccgcga cgcgctggtg cgcatctggc
    55201 aggaagtggc gctggcgcgg gaagatgctt ccccctgttt acgtttgggc gcctttgaaa
    55261 tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt caccgggcaa agcgaaacca
    55321 ttgtgctgtg gcagacgtgg ctacaaccgc tggaattacc ggcggggctg ggaaccgtac
    55381 agcttactgc gggaggcgat attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt
    55441 tcaaagcgcc aggattgctg catattgtcg ggcgcaacgg cggacgtaag ctgaagaaaa
    55501 tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac cacgccactg ctgttttatg
    55561 gagaaacgct gattgcggcg gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg
    55621 agaatggcgt aagttttgtc tggcagaaaa cgcttagtta agtgaaagcc ggataagacg
    55681 catcaaacgt cgcatccggc gaaagtcaat caggattcgc ttaccaccac cgtaccgatt
    55741 tcagggtggc taaagctggt aattttatcc agacgcagct cgcgggtttc gccagcggcc
    55801 tcgacgacca gatactccac atttttgcgg gagactaaat cactggcttt tgcctgcaat
    55861 ttttcgccat ctttcagctc aagtgtcagc attaaatgat gctggcaggc gagctcaaga
    55921 ttatcgtaat catcacaatt aattggttga tacgtatcat tcattgacat aatcgctcac
    55981 cagtaagttt gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc
    56041 ggcagccatt tcattcagca ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa
    56101 gtctccgctg gcgatttccg cgtaccgctt gcgtattaac tcacaatatt tttccacatg
    56161 ccctcctgtc agcactctga cttaaccgtg gatgcaagtc taagcctacg aagataaact
    56221 ctgtttcgca aggtgactat accacactca tttctgcaat atcagcgccg caactgcacg
    56281 tattccgtta caatggcctc ctgattcgaa aggagttttc ttatggcgct taaagcgaca
    56341 atttataaag cgacggttaa tgtggccgat ctcgaccgca accagtttct cgatgcctct
    56401 ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg cttgctggcg
    56461 tggctgaaat atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag
    56521 ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt tgtggattga gctggggctg
    56581 ccggatgagc gacgaattaa gaaagcctgc acccaggccg cagaagtggc gctgtttacc
    56641 tataatagtc gggcggcgca aatctggtgg cagcaaaatc agagcaaatg tgtgcagttt
    56701 gccaatcttt ccgtctggta tctggacgat gaacaactgg cgaaagtaag cgcctttgcc
    56761 gatcgtacca tgacgctgca ggcaacgatt caggatggtg ttatctggtt atcggatgat
    56821 aagaataatc tggaagttaa cttaaccgcc tggcaacaac cttcatgatt gtgatttccc
    56881 gacatgttgc tattcccgat ggtgagcttg agatcaccgc cattcgtgcg cagggcgctg
    56941 gcgggcagca tgttaataag acctcaacgg ctattcatct gcgttttgac attcgggcgt
    57001 ccagcctgcc agagtattac aaagagcgtc tgctcgccgc cagccatcat ttgatcagca
    57061 gtgatggcgt gattgtcatt aaggcacagg aataccgcag tcaggaactg aaccgcgaag
    57121 ccgctctggc ccggctggtg gcagtgatta aagatttaac aaaagaacaa aaagcccgac
    57181 gacccacgcg gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa gcacaaaaat
    57241 caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg ggaataaaaa gaaggaatgg
    57301 atggtgaaaa aagcgatagt gacagcgatg gctgtaatca gcctctttac tctgatggga
    57361 tgtaataatc gggccgaagt cgatacgctt tctccggcgc aggctgccga actgaaaccg
    57421 atgccgcaaa gttggcgcgg cgtgctaccg tgtgccgatt gcgaaggaat cgaaatctct
    57481 ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc gttatctcgg tgctcgtgaa
    57541 gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta
    57601 accgacagca aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct ggagatgctc
    57661 gatcgtgaag gcaatccaat tgaatcgcag ttcaactata cgctggaacc ggcacaatcc
    57721 agtttaccta tgacgccgat gaccctgcga ggcatgtatt tttatatggc tgatgcggcg
    57781 accttcactg attgcgcgac cggaaaacgt ttcatggtag cgaataacgc agagctggag
    57841 cgtagctacc tggctgcgcg cggtcacagt gaaaaaacgg tgttactgtc agtagaaggt
    57901 cactttacgc ttgaggctaa tccggatact ggtgcgccaa ccaaagtatt agcgcccgat
    57961 acggcgggta aattttaccc gaataaggat tgcagtagtt tggggcagta aacctgctta
    58021 aggcagaaac aaacgcaaaa ttgcctgatg cgctgcgctt atcaggccta cagcatataa
    58081 tgcaacattt tgaattagca cgattttgta ggccggataa ggcgtttacg ccgcatccgg
    58141 cattttgtgc acgaggctgg ctttatcact gagtcagccc aacaaagcga cccgccttga
    58201 tataaatccc aggcttcagg tgcccaacat actgtaactg cgtttctggc ttaaacgcag
    58261 agacatcacc aatacgcata tgcatcagat cggcagggct aatccagccc gattgcgcca
    58321 gtgtaagtgg atgcccggat ttggcaaatg catcagtgac aaattccgaa caaaaccacg
    58381 actttttgtc tccttcgcac acactgctta actgcgcttt cgccaggtcg ctgacgcact
    58441 gttggcgaaa atcctcggag aacggattca gtgagcacat ctggcgagtc accataaagg
    58501 gaataaattc gacaatgccg cgatagttat aaccgctatc tttgatttta ttggcaaacg
    58561 cggtgatatc tgtggcttgt tgcggggtaa gatccgggac tcgtaagacg aaaagcttat
    58621 cactatgctt catcgctttt ttaagggaaa caatctggac gccagcgcct gtcgcttctg
    58681 caacgttatt atcacccaga aagattgcaa cgtgactcac agaggaagtg ctgaagacgc
    58741 ggattccaaa tgaggttacc ccaaggcttg aggagaacag caaatcgccg ggtttgagat
    58801 caggtgccgt tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttga
    58861 cagcccatgt ttttgcctcg gcatctactg ctgtggctga tgggtctggc tggctgatat
    58921 caacggtaca agccgaaagt aaaagaaagc tggggagaag caggcggcag tacgcctttg
    58981 gtttatccat tttatacaat ccatgtaaaa aagggccctg aaattcagga ccctttctgg
    59041 catcagcctt taatctgttt caccagatat tcgacgatgt caccagtctt aattaactgt
    59101 ttctcgccgt tgcgacgata tttatattcg atatcgtcgt tgtcgaggtt acggtcgccc
    59161 agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc
    59221 tctttgcggt catccagcag cacttcgata ccttgtgcac gcagttcgct gtacagtttc
    59281 tcagcaagct cttgtacgcg gaaggatttg tgcatgttca tcggcagaat cgccacctgg
    59341 aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt cgtagttctg ctcaatcgcc
    59401 gcagctacca cacgcgttac cccgataccg tagcaaccca tcgtcaggat ttggttacgg
    59461 ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag
    59521 atgtgaccaa cttcgatacc acgtttgatc agcagcgtac cctggccatc cgggcttgga
    59581 tcgccagcca ccacgttacg gatatctgca acttccgggg tagcgacatc gcgatcccag
    59641 ttgataccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc
    59701 gcaacggtac ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga
    59761 ccggctttaa ccacagcacg aatttcttct tcggtcgcga aagtcagcgg gcttgcaacc
    59821 tgcggcagtt tttctgcttt aacttcgttc agctcgtgat caccgcgcac cagcagcgca
    59881 accagcggga agctactgcc ttcaaccgct ttaaccagca gagtcttaac cgttttctca
    59941 atcggcagat tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc
    60001 agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt
    60061 tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca catcgtcttc accgctctgc
    60121 gccagcacct ggaattcgtg agaggcgctg ccgccgatag aaccggtgtc ggcctgtacg
    60181 gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg
    60241 taggtttcct gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat
    60301 tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc
    60361 tgatagaagt tcagcggcag ctgtttgtaa gagctaagct cgttacgaat caggtcagtg
    60421 ataacttctt catgagttgg gccgagtacg aacggacgct cgccacggtc aacaaaacgc
    60481 agcagttccg gaccgtactg ttcccaacga ccactctctt gccacaaatc ggctggctga
    60541 accaccggca tcgacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt
    60601 tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc
    60661 ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt
    60721 gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg
    60781 gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc
    60841 aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa
    60901 aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg tagccagacg gcataggttt
    60961 tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga
    61021 taaacagcgt taactgcgga taacgcttct ccagcgtcaa aagctgcttt tcgacctctg
    61081 cggtaaaact caccgccatc tctgcagctg gcgcactttg cgcatagctg gcactggcat
    61141 cgggaagcga ttcggcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga
    61201 ccagatccag actgccgagc ttcagaatca cgctgccttt atggcaaaca acctctttca
    61261 gctctaccag cgacatgcca attgggttag ggcggaaagt agagcgtgtt gcgaaaaccc
    61321 ccattctggc gttaccgccg aggcgcggtg gacgcacagt cggacgccag ccgccttcca
    61381 tcgtttgatg aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg
    61441 cgtcggcctg gttgtaggaa gcaatgagat gcagttcgcc gttggcgctt tttaccagac
    61501 ctggctggcg cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct
    61561 cgaactggaa actgctcatt tcgccgtaat gttaagcgca gagccgatac atacagcctg
    61621 acgatagcag cccggcgtac cgctggtgac ttcgcagcta tgcagtaata cagcattggc
    61681 tttcatttta gaggcattga tttgcatctg cttacgtgcg gttggaatgc tcggcggaga
    61741 gtcctgatta gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt
    61801 gccgactaat tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg
    61861 ttttgcaggc tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct
    61921 gcttaacatg gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat
    61981 agtatttcct caatgtatga tcaaaacgtc aatattgaat caggagcttg taaaaatgac
    62041 aagacgggca agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaaaatta
    62101 ccagccttta acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcatcaga
    62161 ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct ttgttatctt cacgcgttac
    62221 gatcaggttt acgtatgggg agtctttatc ttcgacaaag ataccgtctt tcgccggagt
    62281 caggccaatc tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc
    62341 cagagaacgc ggcagttgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc
    62401 aacaacatca agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac
    62461 tttttgcagc agcagcagtg aacgaccaag attagttgga tcgtttggca cggcaacctg
    62521 cgaaccatcc tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata
    62581 aacaaaagtg ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag
    62641 gtacggttta tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg
    62701 cagaacatag tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac
    62761 tttctgcgcg acttctgcta cctgctgttc ggcaccaaca atcacgccga ctttaatgtg
    62821 gtttggatct ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc
    62881 cactgccgca aaggttttga atttgaacgc cataccttat tccttcttca attatttatg
    62941 ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat
    63001 taaataaacc agaatgacca gcaataccag taccgtattc atcaccgtcg cgttgtagcc
    63061 gatgtagcca tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat
    63121 cgcggaataa ccgaccaggg taatcagggt gatagttgcc gcattcacca gacccggcaa
    63181 cgcttccggt aacagcactt tacagacgat ctgcattggc gtggcaccca ttgcgcggga
    63241 agcttcaatt aacccggttg ggatctccag cagagcgttc tcgaccatac gggcaataaa
    63301 cggtgctgca ccaacggtta acggaacaat tgctgcctgc aaaccaatcg atgtaccgac
    63361 aataacgcgg gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa
    63421 aatgttcaca atcgcagaaa cggtacgata cagcttcgcg ttagcaataa tttgccccgg
    63481 acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc acaaacccaa aaaatccgga
    63541 tacgaaggtc attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc
    63601 agacataacc cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt
    63661 gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat gccgaattta acgccaccgg
    63721 cgtagtccat ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag
    63781 aaagcagtgg ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt
    63841 cagtaaatgg ctccgtttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg
    63901 tcgactgaat aaacttctgc gccagcggcg ttttcggatg cgagaacact tcacttaccg
    63961 tgtcctgctc gatcagttcg ccattgctga tgaccgccac gcaatcacaa atacgcttca
    64021 caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc cagacggcgg ttaatgtctt
    64081 tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac
    64141 acagcaatac tttgggattg ctggctaatg cacgggcaat cgccacacgt tgtttctgcc
    64201 caccggaaag attcgacggg tagctatcat gcttatcgcc aagaccaacc aatgacagca
    64261 attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt gtccagctcc agcggcagag
    64321 ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa
    64381 tctggcggcg agctttggtc aactcggatt ctgacagcgt ggtcaattcc tggccatcga
    64441 ccagcacgct accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct
    64501 tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga
    64561 cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa
    64621 gttttatcat tgattattta ttatcgtcat taagttagtc gtagcatctc gaatgcctga
    64681 aacgggcaac gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg
    64741 tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga tactagcgtc ccatgcctta
    64801 ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca
    64861 ccattaatgt cgatcacggc tatgtccatg agatcgacaa ctttgaattt atcgacggtg
    64921 ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc
    64981 agtctggcat tgctcgcggt aaatttaccg aagcacagtt tgaaacgctg accgagtgga
    65041 tggactggtc gctggcggac cgagatgtcg atctggatgg tatctattat tgcccgcatc
    65101 atccgcaggg gagtgttgaa gagtttcgcc aggtctgcga ttgccgcaaa ccacatccgg
    65161 ggatgctttt gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg
    65221 gcgataaatt agaagatatg caggcagcgg ttgcggcgaa cgtgggaaca aaagtgctgg
    65281 tgcgtacggg taaacctatt acacctgaag cagaaaacgc ggcagattgg gtgttaaata
    65341 gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa accggcgcaa tgattaaaag
    65401 atgaacggtt gaaataaaaa tgcatttttc cgcttgtctt cctgagccga ctccctataa
    65461 tgcgcctcca tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa
    65521 gagaaaaaat cctgaaattc agggttgact ctgaaagagg aaagtgtgat atacgccacc
    65581 tcgcgacagt gagctgaaag ctgcgtcgca actgctcttt aacaatttat cagacaatct
    65641 gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga cgataaatga ataccaagtc
    65701 tcaagagtga acacgtaatt cattacgaag tttaattctt tgagcatcaa acttttaaat
    65761 tgaagagttt gatcatggct cagattgaac gctggcggca ggcctaacac atgcaagtcg
    65821 aacggtaaca ggaagcgctt gctgcttcgc tgacgagtgg cggacgggtg agtaatgtct
    65881 gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac cgcataacgt
    65941 cgcaagacca aagaggggga ccttcgggcc tcttgccatc ggatgtgccc agatgggatt
    66001 agcttgttgg tggggtaacg gctcacc
//
LOCUS       DBKELA010000007        65613 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.007, whole genome shotgun sequence.
ACCESSION   DBKELA010000007 DBKELA010000000
VERSION     DBKELA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.007"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACUQR0_000427"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACUQR0_000427"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HHV9107615.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACUQR0_000428"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACUQR0_000428"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HHV9107616.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACUQR0_000429"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACUQR0_000429"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HHV9107617.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACUQR0_000430"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACUQR0_000430"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HHV9107618.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACUQR0_000431"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACUQR0_000431"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HHV9107619.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACUQR0_000432"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACUQR0_000432"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HHV9107620.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACUQR0_000433"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACUQR0_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HHV9107621.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACUQR0_000434"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACUQR0_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HHV9107622.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACUQR0_000435"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACUQR0_000435"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HHV9107623.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACUQR0_000436"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACUQR0_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HHV9107624.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACUQR0_000437"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACUQR0_000437"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HHV9107625.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACUQR0_000438"
     CDS             complement(15665..16702)
                     /locus_tag="ACUQR0_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HHV9107626.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACUQR0_000439"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACUQR0_000439"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HHV9107627.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACUQR0_000440"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACUQR0_000440"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HHV9107628.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACUQR0_000441"
     CDS             19444..19884
                     /locus_tag="ACUQR0_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HHV9107629.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACUQR0_000442"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACUQR0_000442"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HHV9107630.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACUQR0_000443"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACUQR0_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACUQR0_000444"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACUQR0_000444"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HHV9107631.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACUQR0_000445"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACUQR0_000445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HHV9107632.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACUQR0_000446"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACUQR0_000446"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HHV9107633.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACUQR0_000447"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACUQR0_000447"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HHV9107634.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACUQR0_000448"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACUQR0_000448"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HHV9107635.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACUQR0_000449"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACUQR0_000449"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HHV9107636.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACUQR0_000450"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACUQR0_000450"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HHV9107637.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACUQR0_000451"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACUQR0_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HHV9107638.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="ACUQR0_000452"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="ACUQR0_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HHV9107639.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACUQR0_000453"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACUQR0_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HHV9107640.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACUQR0_000454"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACUQR0_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HHV9107641.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACUQR0_000455"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACUQR0_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HHV9107642.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACUQR0_000456"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACUQR0_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HHV9107643.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACUQR0_000457"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACUQR0_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HHV9107644.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="ACUQR0_000458"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="ACUQR0_000458"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HHV9107645.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="ACUQR0_000459"
     CDS             37412..37681
                     /locus_tag="ACUQR0_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HHV9107646.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="ACUQR0_000460"
     CDS             complement(37684..38043)
                     /locus_tag="ACUQR0_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HHV9107647.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="ACUQR0_000461"
     CDS             38184..38810
                     /locus_tag="ACUQR0_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HHV9107648.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="ACUQR0_000462"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="ACUQR0_000462"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HHV9107649.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACUQR0_000463"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACUQR0_000463"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HHV9107650.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="ACUQR0_000464"
     CDS             41650..42315
                     /locus_tag="ACUQR0_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HHV9107651.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="ACUQR0_000465"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="ACUQR0_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HHV9107652.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="ACUQR0_000466"
     CDS             complement(42949..44166)
                     /locus_tag="ACUQR0_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9107653.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="ACUQR0_000467"
     CDS             44300..45349
                     /locus_tag="ACUQR0_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHV9107654.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="ACUQR0_000468"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="ACUQR0_000468"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HHV9107655.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="ACUQR0_000469"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="ACUQR0_000469"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9107656.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="ACUQR0_000470"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="ACUQR0_000470"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HHV9107657.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="ACUQR0_000471"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="ACUQR0_000471"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HHV9107658.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="ACUQR0_000472"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="ACUQR0_000472"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HHV9107659.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="ACUQR0_000473"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="ACUQR0_000473"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HHV9107660.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="ACUQR0_000474"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="ACUQR0_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HHV9107661.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="ACUQR0_000475"
     CDS             complement(51544..51903)
                     /locus_tag="ACUQR0_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HHV9107662.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="ACUQR0_000476"
     CDS             complement(51900..52175)
                     /locus_tag="ACUQR0_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HHV9107663.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="ACUQR0_000477"
     CDS             complement(52234..53358)
                     /locus_tag="ACUQR0_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9107664.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="ACUQR0_000478"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="ACUQR0_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HHV9107665.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="ACUQR0_000479"
     CDS             complement(56090..57157)
                     /locus_tag="ACUQR0_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9107666.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57220..57333)
                     /gene="yhiY"
                     /locus_tag="ACUQR0_000480"
     CDS             complement(57220..57333)
                     /gene="yhiY"
                     /locus_tag="ACUQR0_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HHV9107667.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(57523..59145)
                     /locus_tag="ACUQR0_000481"
     CDS             complement(57523..59145)
                     /locus_tag="ACUQR0_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HHV9107668.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="ACUQR0_000482"
     CDS             complement(59407..60996)
                     /locus_tag="ACUQR0_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HHV9107669.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61414..62412
                     /locus_tag="ACUQR0_000483"
                     /pseudo
     CDS             61414..62412
                     /locus_tag="ACUQR0_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386465.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="ACUQR0_000484"
     CDS             complement(62499..62975)
                     /locus_tag="ACUQR0_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107670.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="ACUQR0_000485"
     CDS             complement(62968..63732)
                     /locus_tag="ACUQR0_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HHV9107671.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="ACUQR0_000486"
     CDS             63978..64739
                     /locus_tag="ACUQR0_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHV9107672.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65358)
                     /locus_tag="ACUQR0_000487"
     CDS             complement(64846..65358)
                     /locus_tag="ACUQR0_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107673.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(65497..>65613)
                     /locus_tag="ACUQR0_000488"
     CDS             complement(65497..>65613)
                     /locus_tag="ACUQR0_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107674.1"
                     /translation="GEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        15936 a        17620 c        16162 g        15895 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccatagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact gaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcc
//
LOCUS       DBKELA010000008        64593 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.008, whole genome shotgun sequence.
ACCESSION   DBKELA010000008 DBKELA010000000
VERSION     DBKELA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.008"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..575
                     /locus_tag="ACUQR0_000489"
     CDS             <1..575
                     /locus_tag="ACUQR0_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9107675.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1629
                     /gene="ybhA"
                     /locus_tag="ACUQR0_000490"
     CDS             811..1629
                     /gene="ybhA"
                     /locus_tag="ACUQR0_000490"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HHV9107676.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1630..2688)
                     /gene="modC"
                     /locus_tag="ACUQR0_000491"
     CDS             complement(1630..2688)
                     /gene="modC"
                     /locus_tag="ACUQR0_000491"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HHV9107677.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2691..3380)
                     /gene="modB"
                     /locus_tag="ACUQR0_000492"
     CDS             complement(2691..3380)
                     /gene="modB"
                     /locus_tag="ACUQR0_000492"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HHV9107678.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3380..4153)
                     /gene="modA"
                     /locus_tag="ACUQR0_000493"
     CDS             complement(3380..4153)
                     /gene="modA"
                     /locus_tag="ACUQR0_000493"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9107679.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="ACUQR0_000494"
     CDS             complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="ACUQR0_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HHV9107680.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4598..5386
                     /gene="modE"
                     /locus_tag="ACUQR0_000495"
     CDS             4598..5386
                     /gene="modE"
                     /locus_tag="ACUQR0_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HHV9107681.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5454..6926
                     /gene="modF"
                     /locus_tag="ACUQR0_000496"
     CDS             5454..6926
                     /gene="modF"
                     /locus_tag="ACUQR0_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HHV9107682.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7187..8203
                     /gene="galE"
                     /locus_tag="ACUQR0_000497"
     CDS             7187..8203
                     /gene="galE"
                     /locus_tag="ACUQR0_000497"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HHV9107683.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8213..9259
                     /gene="galT"
                     /locus_tag="ACUQR0_000498"
     CDS             8213..9259
                     /gene="galT"
                     /locus_tag="ACUQR0_000498"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HHV9107684.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9263..10411
                     /gene="galK"
                     /locus_tag="ACUQR0_000499"
     CDS             9263..10411
                     /gene="galK"
                     /locus_tag="ACUQR0_000499"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HHV9107685.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10405..11445
                     /gene="galM"
                     /locus_tag="ACUQR0_000500"
     CDS             10405..11445
                     /gene="galM"
                     /locus_tag="ACUQR0_000500"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HHV9107686.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11648..12400
                     /gene="gpmA"
                     /locus_tag="ACUQR0_000501"
     CDS             11648..12400
                     /gene="gpmA"
                     /locus_tag="ACUQR0_000501"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HHV9107687.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="ACUQR0_000502"
     CDS             complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="ACUQR0_000502"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HHV9107688.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13935..14315
                     /locus_tag="ACUQR0_000503"
     CDS             13935..14315
                     /locus_tag="ACUQR0_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HHV9107689.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14429..15370
                     /gene="zitB"
                     /locus_tag="ACUQR0_000504"
     CDS             14429..15370
                     /gene="zitB"
                     /locus_tag="ACUQR0_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HHV9107690.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="ACUQR0_000505"
     CDS             complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="ACUQR0_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HHV9107691.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="ACUQR0_000506"
     CDS             complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="ACUQR0_000506"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HHV9107692.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17416..17491)
                     /locus_tag="ACUQR0_000507"
     tRNA            complement(17416..17491)
                     /locus_tag="ACUQR0_000507"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17456..17458),aa:Lys,seq:ttt)
     gene            complement(17495..17570)
                     /locus_tag="ACUQR0_000508"
     tRNA            complement(17495..17570)
                     /locus_tag="ACUQR0_000508"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17535..17537),aa:Val,seq:tac)
     gene            complement(17622..17697)
                     /locus_tag="ACUQR0_000509"
     tRNA            complement(17622..17697)
                     /locus_tag="ACUQR0_000509"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17662..17664),aa:Lys,seq:ttt)
     gene            complement(17700..17775)
                     /locus_tag="ACUQR0_000510"
     tRNA            complement(17700..17775)
                     /locus_tag="ACUQR0_000510"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17740..17742),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACUQR0_000511"
     tRNA            complement(17912..17987)
                     /locus_tag="ACUQR0_000511"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACUQR0_000512"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACUQR0_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HHV9107693.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACUQR0_000513"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACUQR0_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HHV9107694.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACUQR0_000514"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACUQR0_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HHV9107695.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACUQR0_000515"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACUQR0_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HHV9107696.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACUQR0_000516"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACUQR0_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HHV9107697.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACUQR0_000517"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACUQR0_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HHV9107698.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACUQR0_000518"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACUQR0_000518"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HHV9107699.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACUQR0_000519"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACUQR0_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HHV9107700.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACUQR0_000520"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACUQR0_000520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HHV9107701.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACUQR0_000521"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACUQR0_000521"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HHV9107702.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACUQR0_000522"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACUQR0_000522"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HHV9107703.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27908..30541)
                     /gene="mngB"
                     /locus_tag="ACUQR0_000523"
                     /pseudo
     CDS             complement(27908..30541)
                     /gene="mngB"
                     /locus_tag="ACUQR0_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30559..32475)
                     /gene="mngA"
                     /locus_tag="ACUQR0_000524"
     CDS             complement(30559..32475)
                     /gene="mngA"
                     /locus_tag="ACUQR0_000524"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HHV9107704.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32645..33367
                     /gene="mngR"
                     /locus_tag="ACUQR0_000525"
     CDS             32645..33367
                     /gene="mngR"
                     /locus_tag="ACUQR0_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HHV9107705.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33470..34339)
                     /gene="sucD"
                     /locus_tag="ACUQR0_000526"
     CDS             complement(33470..34339)
                     /gene="sucD"
                     /locus_tag="ACUQR0_000526"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HHV9107706.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34339..35505)
                     /gene="sucC"
                     /locus_tag="ACUQR0_000527"
     CDS             complement(34339..35505)
                     /gene="sucC"
                     /locus_tag="ACUQR0_000527"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HHV9107707.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35599..36816)
                     /gene="odhB"
                     /locus_tag="ACUQR0_000528"
     CDS             complement(35599..36816)
                     /gene="odhB"
                     /locus_tag="ACUQR0_000528"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HHV9107708.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASVDGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36831..39632)
                     /gene="sucA"
                     /locus_tag="ACUQR0_000529"
     CDS             complement(36831..39632)
                     /gene="sucA"
                     /locus_tag="ACUQR0_000529"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HHV9107709.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39933..40649)
                     /gene="sdhB"
                     /locus_tag="ACUQR0_000530"
     CDS             complement(39933..40649)
                     /gene="sdhB"
                     /locus_tag="ACUQR0_000530"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HHV9107710.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40665..42431)
                     /gene="sdhA"
                     /locus_tag="ACUQR0_000531"
     CDS             complement(40665..42431)
                     /gene="sdhA"
                     /locus_tag="ACUQR0_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HHV9107711.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42431..42778)
                     /gene="sdhD"
                     /locus_tag="ACUQR0_000532"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42431..42778)
                     /gene="sdhD"
                     /locus_tag="ACUQR0_000532"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HHV9107712.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42772..43176)
                     /gene="sdhC"
                     /locus_tag="ACUQR0_000533"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42772..43176)
                     /gene="sdhC"
                     /locus_tag="ACUQR0_000533"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HHV9107713.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43557..43664
                     /locus_tag="ACUQR0_000534"
     CDS             43557..43664
                     /locus_tag="ACUQR0_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107714.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43870..45153
                     /gene="gltA"
                     /locus_tag="ACUQR0_000535"
     CDS             43870..45153
                     /gene="gltA"
                     /locus_tag="ACUQR0_000535"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HHV9107715.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45544..46110
                     /locus_tag="ACUQR0_000536"
     CDS             45544..46110
                     /locus_tag="ACUQR0_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9107716.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46161..48620
                     /locus_tag="ACUQR0_000537"
     CDS             46161..48620
                     /locus_tag="ACUQR0_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9107717.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48635..49366
                     /locus_tag="ACUQR0_000538"
     CDS             48635..49366
                     /locus_tag="ACUQR0_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA];
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HHV9107718.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49363..50439
                     /locus_tag="ACUQR0_000539"
                     /pseudo
     CDS             49363..50439
                     /locus_tag="ACUQR0_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50591..51637
                     /locus_tag="ACUQR0_000540"
     CDS             50591..51637
                     /locus_tag="ACUQR0_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HHV9107719.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51634..52425)
                     /gene="nei"
                     /locus_tag="ACUQR0_000541"
     CDS             complement(51634..52425)
                     /gene="nei"
                     /locus_tag="ACUQR0_000541"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HHV9107720.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52461..53195)
                     /gene="pxpA"
                     /locus_tag="ACUQR0_000542"
     CDS             complement(52461..53195)
                     /gene="pxpA"
                     /locus_tag="ACUQR0_000542"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HHV9107721.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53185..54117)
                     /gene="pxpC"
                     /locus_tag="ACUQR0_000543"
                     /pseudo
     CDS             complement(53185..54117)
                     /gene="pxpC"
                     /locus_tag="ACUQR0_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54111..54767)
                     /gene="pxpB"
                     /locus_tag="ACUQR0_000544"
     CDS             complement(54111..54767)
                     /gene="pxpB"
                     /locus_tag="ACUQR0_000544"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HHV9107722.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54790..55533)
                     /gene="ybgI"
                     /locus_tag="ACUQR0_000545"
     CDS             complement(54790..55533)
                     /gene="ybgI"
                     /locus_tag="ACUQR0_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HHV9107723.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55804..57285
                     /gene="dtpD"
                     /locus_tag="ACUQR0_000546"
     CDS             55804..57285
                     /gene="dtpD"
                     /locus_tag="ACUQR0_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HHV9107724.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57435..58853)
                     /gene="phrB"
                     /locus_tag="ACUQR0_000547"
     CDS             complement(57435..58853)
                     /gene="phrB"
                     /locus_tag="ACUQR0_000547"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HHV9107725.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58850..59359)
                     /locus_tag="ACUQR0_000548"
     CDS             complement(58850..59359)
                     /locus_tag="ACUQR0_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HHV9107726.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59488..59775
                     /locus_tag="ACUQR0_000549"
     CDS             59488..59775
                     /locus_tag="ACUQR0_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HHV9107727.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59830..60210)
                     /locus_tag="ACUQR0_000550"
     CDS             complement(59830..60210)
                     /locus_tag="ACUQR0_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107728.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60213..62357)
                     /locus_tag="ACUQR0_000551"
     CDS             complement(60213..62357)
                     /locus_tag="ACUQR0_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HHV9107729.1"
                     /translation="MTDAQSHSTRYGYDDSGHLTEVILADGRTVNYQSDAAGRLVKYT
                     SPMGRITRWQRDGQGRVRSRTDAMGRRTAFGYDAYGRLVTLTNENGERYRFRHDVLDR
                     LAEQINPDGCRQSYRYNALNAVTEVVFTGDRGGEIRHRLARDAAGRLTAKETADGRTE
                     YVHDAADQLLEIRRQRHETDAPEVIRFSYDRLGRMLSEETAQGILTHQYDKLGNRTAT
                     TFPDGRTQRHLYYGSGHLQQINLDREVISEFTRDHLHREVLRSQGRLSTRQLYDPGGR
                     LKRRETYSGMRGVVPETFTDRQYSYSGEDELLKTRHSRRGETDYFYDPTGRITACRSE
                     DEGYLASWQYDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEFGRAVEKEG
                     RSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNRTKFLWDGM
                     RLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGAPEEMTDSD
                     GKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYDPDVGRFMV
                     TDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVTVRSRSDAE
                     QLFMDRYLGHNYKNMTGESGPSTKNLMEYLTGNKTKAGSYHWDDIKDPSVTKPSYRVS
                     GHGLGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(62493..>64593)
                     /locus_tag="ACUQR0_000552"
     CDS             complement(62493..>64593)
                     /locus_tag="ACUQR0_000552"
                     /inference="COORDINATES: protein motif:HMM:NF040127.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HHV9107730.1"
                     /translation="IEGYILTGSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQE
                     EAQRQEDARTFRDVAGEYLEMAGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDG
                     VTHFVSELWQPTVASAAPGSTPTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALN
                     LLNPGGMLETAFQGVNAVIGSISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGAR
                     CTCEARVVNEPGNGAFVSPDVRIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLS
                     KIACFAASVGMSMLTQKATSALPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYS
                     SRDERTEGMFGQGWSVMYKVSLVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSP
                     GEGLAVRRGEQGHWLISSDDGQCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGR
                     IVEISGEQQRPCIRLYYELAAHPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDS
                     TGHLLREFAYDENHCMTLHRQPGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLA
                     WDLAQRRLCVTDGLGRTRYHQWDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQG
                     GKWRYVYDLQGHITETHDPLGRVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLA
                     VTDPLQQNTRYQYDHHG"
BASE COUNT        15624 a        17495 c        15817 g        15657 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg caaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaattgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 gccacttgta attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat
     1021 ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc
     1081 ctgcaactca ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgat
     1141 gcaatggtct atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc
     1201 ctgccgccgg aacagcgtcc gactttcaca caagtcgctt ctctggctga aacgacgcaa
     1261 caagttaacg ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat
     1321 tttggtaagc atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag
     1381 gttgatattg cacacggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg
     1441 caaggttggt cgatggaaaa cgtcgtggca ttcggcgata actttaatga tatcagtatg
     1501 ctggaagccg ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc
     1561 gccaacattg tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac
     1621 ctgatttaat caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg
     1681 tttgatcgcc agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc
     1741 gacttccagc tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg
     1801 caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc
     1861 ctgaatgcgg atacgtagcg cagcttgcag cggttcgccc agcttattga cccacaaatg
     1921 ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gcaccgtcac
     1981 tttcagaatg ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca
     2041 cacttcctcc agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc
     2101 tgccagatgg aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc
     2161 ccgtgtcagc cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc
     2221 cagcggttca tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac
     2281 gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc
     2341 taaaagcgcc accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag
     2401 attgccacgc actttgtaat gcgggaacaa ccgcgcatcc tgaaaaacat agccaacacg
     2461 acgcttttcc ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag
     2521 gacaatccgc cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt
     2581 tccggcaccg gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat
     2641 ggtcaggcaa tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc
     2701 gcccgttcac ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc
     2761 gcaatagaaa taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg
     2821 gtatacatgg cagaaggaat ggttcgcgtt tcgctaggaa tgttcgacac aaaggtgatg
     2881 gttgcaccaa actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc
     2941 ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt
     3001 cttgcggcct gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc
     3061 atcagcggaa acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag
     3121 gtaataccaa accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact
     3181 aataataagt aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg
     3241 agcagagctt tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac
     3301 ggcaggctaa acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat
     3361 tctggatcgg tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg
     3421 cctgcggtcc cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca
     3481 caacagcaac cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt
     3541 ttaccccttt gctggcaact gcgtcagaac cgtagacaat gcccagaggc gcttcgttac
     3601 gttcgaccag cgccagcgcc ccacgaacat cttccgctgg ggccagtttc ggagagagcg
     3661 tatcccatgc gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt
     3721 ccggatcgcc aaccgccagg cgaccgccat tcagcagcga agtccagtta gttttgctgt
     3781 cgatggtgaa atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga
     3841 gcagtgtctg acgcgtagca gtatcgatcg cttttttatc aaccgcataa tccatccatt
     3901 tctgatcggc agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag
     3961 tagatgacga agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag
     4021 caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat
     4081 ctgccagtgc attgccagca actgcgaaag agagtgccgc cccggcaaac aagttcaacc
     4141 atttacgagc cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta
     4201 actcggaatt ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg
     4261 gcaggaaaag caggaagttg agaaaaagaa aatgcccgac taagcgggca ctcagggaat
     4321 caatgatttt gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca
     4381 ccaagaccgt aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg
     4441 aataccagac tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt
     4501 ctaaccgcta agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg
     4561 ttttccgtca caataagact tttgccagga cattgttatg caggccgaaa tccttctcac
     4621 ccttaagctc caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat
     4681 tgcgctttcc ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc
     4741 ctgggatgcc attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac
     4801 aggcggtaaa ggtggcggag gcgcagtact gacccgctat ggtcagcgac tgattcagct
     4861 ctatgactta ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc
     4921 cctgccgctg aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg
     4981 taaccagtgg ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga
     5041 tgtcttactg gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc
     5101 acgtctgggg ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg
     5161 cattactcag gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag
     5221 tcatattgag cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac
     5281 actgtgcgcc acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac
     5341 ggcctacttt aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt
     5401 tgttatgaaa cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat
     5461 cgttgcaaat tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc
     5521 agctaacgtt aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agtggaaagt
     5581 cggcgctggc ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc
     5641 agttttccca catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat
     5701 ggcaacggaa taacaccgat atgctcggcc ctagcgaaga ttacaccgga cgcactacgg
     5761 ctgagattat tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt
     5821 tcggtattac cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa
     5881 aaaccctgct gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt
     5941 tcgatggcct ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc
     6001 agtccggtat tactttggta ctggtgctca atcgcttcga taagatcccg gaatttgtcc
     6061 agtttgctgg cgtgctggcg gattgtacgt tagcggaaac tggcgctaaa gaggaactgc
     6121 tccagcaagc actcgtcgcg caacttgcgc atagtgaaca gcttgaaggt gtgcaactgc
     6181 cggagccgga tgaaccttca gcacgtcacg ccttacccgc caacgaatcg cgcattgtgc
     6241 tgaacaatgg cgtggtttct tataatgatc gccccattct taataacctt agctggcagg
     6301 tgaatccagg cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat
     6361 taagcctgat tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggcc
     6421 gacgtcgcgg cagcagcgaa accatctggg acatcaaaaa gcatatcggt tacgtcagca
     6481 gtagtttgca tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct
     6541 attttgattc gattggcatt tatcaggtcg tttcggatcg ccagcaaaaa ctggtgcagc
     6601 agtggctgga tattctcggc attgataaac acacggctga cgctccgttc catagtcttt
     6661 cctggggaca gcagcgtctg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc
     6721 ttattctcga tgaaccacta caggggcttg atccgctcaa tcgccaactt atccgccgtt
     6781 ttgttgatgt gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg
     6841 aagatgcgcc tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc
     6901 gctatgtgct gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcggcctaa
     6961 gcgcactctc cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat
     7021 aaaaagcgca atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac
     7081 ggctaaattc ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt
     7141 tgttatgcta tggttatttc ataccacaag cctaatggag cgaattatga gagttctggt
     7201 taccggtggt agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca
     7261 tgatgtcatc attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga
     7321 gcgtttaggc ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat
     7381 gaccgagatc ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc
     7441 cgtcgccgaa tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct
     7501 gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc
     7561 caccgtttat ggcgatcagc cccaaattcc atacgttgaa agcttcccga ccggcacacc
     7621 gcaaagccct tatggcaaaa gcaaattgat ggtggaacag atcctcaccg acctgcaaaa
     7681 agcccagccg gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc
     7741 gtcgggcgat atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc
     7801 ccaagttgct gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga
     7861 agatggtact ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt
     7921 ggcgatggaa aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt
     7981 aggcagcagc gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa
     8041 ctatcatttt gcaccgcgtc gcgaaggcga ccttccggcc tactgggcgg acgccagcaa
     8101 agccgaccgt gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac
     8161 ctggcactgg cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca
     8221 atttaatctc gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct
     8281 ggtttcaccg caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca
     8341 ggtgttacct gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg
     8401 cgataaaaac cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat
     8461 gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg
     8521 cggtaccagc cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag
     8581 cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa
     8641 aacgtacccg tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc
     8701 gcatccgcac ggacagattt gggcaaatag cttcctgcct aacgaagctg agcgcgaaga
     8761 ccgcctgcaa aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca
     8821 gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt
     8881 gccttactgg gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttgcg
     8941 gatcaccgat ttgaccgacg cccagcgcag cgatttggcg ctggcgttga aaaagctgac
     9001 cagtcgttat gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc
     9061 accatttaat ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct
     9121 gctgcgctcc gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca
     9181 gcgagacctg accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt
     9241 tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa
     9301 cgcatttggc taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg
     9361 tgaacacacc gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt
     9421 gatcagctgt gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa
     9481 tcagctcgac gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc
     9541 gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt
     9601 ggacatggtg atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact
     9661 ggaagtcgcg gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca
     9721 aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat
     9781 ggatcagcta atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgccgctc
     9841 actggggact aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa
     9901 cttcaaacgt accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg
     9961 tgcgcgtttc ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaacgctgt
    10021 tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc
    10081 ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt
    10141 gatggccgag tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga
    10201 cactctggta gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg
    10261 cggcggattt ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca
    10321 gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg
    10381 taaaccatca caaggagcag gacagtgctg aacgaaactc ccgcactggc acccgatggt
    10441 cagccgtacc gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac
    10501 tggggtgcga ctttactttc cgcccgtatt ccgctttccg atggcagcgt ccgcgaggcg
    10561 ctgctcggct gtgccagtcc ggaatgctat caggatcagg ctgcgtttct gggggcctct
    10621 attggtcgtt atgccaaccg catcgccaat agtcgctata cctttgacgg tgaaaccgtg
    10681 acgctttcgc caagccaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa
    10741 cgtcgctggc agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat
    10801 gatggcgatc agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac
    10861 gataaccgta tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg
    10921 actaatcacg tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg
    10981 cagattctgg cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg
    11041 aaatctgtcg ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt
    11101 cttgccgacg acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc
    11161 aaaggcgatg gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg
    11221 aaggtctaca ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca
    11281 ccgtcgcggg gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgagttt
    11341 ctgccggaca gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc
    11401 gaagagtatt ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc
    11461 atgagggctt tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc
    11521 aatattcaca acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg
    11581 ctgttgcttc gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag
    11641 taagcatatg gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa
    11701 agaaaaccgt ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc
    11761 aaaagcagca ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc
    11821 tgtgctgaaa cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg
    11881 gctgcccgtt gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct
    11941 gaacaaagcg gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg
    12001 ttttgcagtg actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc
    12061 gcgttacgcg aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat
    12121 tgaccgcgtg atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg
    12181 cgtaatcatc gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat
    12241 gagcgaagaa gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt
    12301 cgacgagaat ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc
    12361 gaaagcagca gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat
    12421 gagaaagccg actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca
    12481 ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact
    12541 ctgacggcgc atccgacaat taaaccttac ccgcgacgcg cttttactgc attcaccagt
    12601 tgacgtaaca gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg
    12661 taggccagcg gctccccgct atcgaggctc tgattgcctt ccaccagatg gctttccacc
    12721 atcacgccaa taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca
    12781 tccatctgct ttttgaattg tttggacgaa ttagcatggc tgaaatctat catcacctga
    12841 gctggcagac ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag
    12901 ttaggctctt taccgccgcg cagaatgata tggcaatcgc cgttaccact ggtattcaca
    12961 atcgccgaat gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta
    13021 atggcatcga tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa
    13081 gaaagaccag acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg
    13141 ccccagctca tcaggtcagc gagatactga ggagtgatca tatcgagaaa ctcacccgcc
    13201 gctggcagac cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg
    13261 ttgatctgga agctgttatc catatgcgga tcgttaatca accctttcca gcccaccgtg
    13321 gtacgcggct tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca
    13381 cgcagcgcca gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac
    13441 gggccaatca ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt
    13501 cgggcatggg caaccgtatt cgcggcattt tcagttgcgg ggaatttttc cagcaatgcg
    13561 acaggaggaa gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg
    13621 tctgttccag tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc
    13681 gtcagaatgt gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa
    13741 aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt
    13801 gtttgttaat tatttcaaga ttctctgctg cgtttcataa actggctgaa atcttaaacc
    13861 aatgccttat attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat
    13921 aacaggagca tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa
    13981 gccttgccag cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg
    14041 caaacgccgc agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa
    14101 ataacgtcga caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg
    14161 gttcttcaat gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca
    14221 aagatggtcg atgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg
    14281 atgtcgatac caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga
    14341 gagcggagtc tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta
    14401 agtgagaata attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca
    14461 cctgccagaa gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt
    14521 tatgctggta gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc
    14581 gggtcatatg ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc
    14641 acgtcgtcct cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc
    14701 gtttgtgaat gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga
    14761 acgcttccgc acgccgcgtc cggtcgaggg aggcatgatg atggcaattg ccgtggctgg
    14821 gttgctggca aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct
    14881 caacgtacga gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat
    14941 tattgccgcg ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat
    15001 actggtgtcg cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga
    15061 attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg
    15121 ggaaatcccg gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc
    15181 ggtgatgacg ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca
    15241 gatccaacac tacctgatgg atcactatca gattgagcac accaccattc agatggaata
    15301 tcaaccttgt cataggccag actgccacct gaatgaaggg gtgtctggcc attcacatca
    15361 tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg
    15421 gctgccgttg agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc
    15481 ctgaagtgca aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc
    15541 cacatactta cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc
    15601 ccagaacggg aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg
    15661 catgatcatg actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat
    15721 cagaccaatc gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca
    15781 gcgaattttc aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta
    15841 aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa
    15901 gaaaataatg ccaaacaagg taacgttgat caggccaaag aagtagttgc tgatcttctc
    15961 cagactggca aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga
    16021 gagatcataa ccgcccgccc ctattggtat atgtaccagg atattctgca cactaaaaaa
    16081 atccatcttt cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag
    16141 ccgcaaaatc cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctctcatcga
    16201 catgaacctc gtgattactt ccttcctgtt ctaatgcctc tgcgatagcc tgaaggtcat
    16261 tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg
    16321 gtgcttccag taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat
    16381 cggttgccac aataagcctc tgatgaggca atgcttttgc agcagcgatc agttgactgg
    16441 tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta
    16501 tggcagcatc cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat
    16561 gcacaatgca ggcaccctgc cagcataaaa tgtctccacc cgtctgtttt tgcacgtaac
    16621 gccccagatg tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa
    16681 taagttcgac ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag
    16741 aagtgttggc gtagacgacg acagtacgat cgggatgggc atcgtaaaat gcgttaaatt
    16801 cttcaacagg gcagccgaga tccagtgaac actcaacctg aagtgtcggc atcagaattg
    16861 ttttttccgg actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca
    16921 aagtagaagc gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc
    16981 caccggtttc ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca
    17041 ccgcattacg ttcttttagc aaacgtttta tcttctcgcg gtaatacgct ttttcatcaa
    17101 tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta
    17161 cgctcatctt accatctcgt tttacaggct taacgttaaa accgacatta gcgtaacatt
    17221 cgctgttatc ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa
    17281 gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg
    17341 atgtcatcag gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag
    17401 ggcgcttttt tacattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    17461 tcaactgctc taccaactga gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg
    17521 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactgcgc
    17581 tggatactac aaactacttc caccgaaccc accgtaatta gtggtgggtc gtgcaggatt
    17641 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccggt
    17701 ggtgggtgat gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag
    17761 ctgagctaat cacccgacac tacgctggat actacaacta actatgttag tggtgggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agccgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acctgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgctgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggcctg
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgcctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgcccatttc caagacttat acattgataa atatcaattt ctacaaatta
    27421 tgttaatcat gtttcaacaa ggtaacttta gtgatattta tgtttaaaat gtgtgaatta
    27481 taagactgaa attagtttcg gttggcaatt aaagataaaa agagacgaag caaaaacagc
    27541 acggttgacc ttaattcttt ttttggtcac tggctaatac gacgcagagt ttcaggatta
    27601 ttaataaagg cataaatctc ccccccgccg atattaacat ttaattaact tataattata
    27661 ttataattaa ctcacccgtg gttattattt tgttaaaaat aaagccacat atacgtagct
    27721 tgagagtgaa gataaacaca aagttacctg atgcgctacg cttgccagtg tctacatcat
    27781 ctctgcaata tattgaattt gcctgctttt gtagaccgaa taaagcgttc atgccgtatc
    27841 cggcatgaac aaagcgccct ttgtcaacaa tctggccgcg catatgtggc ttcggtttga
    27901 ttgctattca ggcaagccgg taactgaacg tccgtgactg gccgggtaaa aaaggccccg
    27961 atagatttga accttgattt tcctcggtgg taatgtgttc atccatcatc gtttctgagc
    28021 aagaaatcac ctcgcgactg aaagcaacag tcgcatcaca ggttgctgat tcagccggat
    28081 taaacagccg taaaatcact tcttgtcggt cttcagcttt cttaagtgcg cttatcaggc
    28141 atcccactgg gggcattttc aacaaactat aactttccgg cacgttgaat ccggctttgt
    28201 tgagcttcat cgcatcccat gggattttgt tgtaacactg tactggagtc agccatgctc
    28261 gcgcctgctg agctacacca gcggcggttg gcgtaccggt ataactcaat aaacttaggc
    28321 gacaagaaag cagaccacgt agttgtgagt ccgggactgg cattttaatt cccgaaggcc
    28381 gcctaggcct taaaagcaga tcttctttcc ccagtaagcc cacgccgcgc agcaacgtaa
    28441 tggcaaaggt tttcttctct tcaccgatga cttcaaattc acgtagccct tcgctaaaga
    28501 cagccatgcc gttacgccct tcctgtaagg ctgcatagtt gagcatattc cataccggaa
    28561 ccggcgcttc tttccagcct tcttgctgcc agttgttcat tgcactgtcg ttcacggggc
    28621 gcgttagcga accaaactgg gtatctgcca gaacactatc ggtgttaaaa gatgtaggga
    28681 tcaggacgcg aaggcgatga tcgtcagcct ggttatcaag gttgatatcc acatcaatgc
    28741 gcctgttgtt atgactaaga gtgacaacca actcgacatc tactctgcca gtggattgtc
    28801 ttgcgctgcg ttgttgcaga ttacgcggca ccgccatttc atagcggata acagccctgc
    28861 tctgccaggc ctcatgaata atctcgcatt gcggtttcgc attcgccgaa gtaattaccc
    28921 actcttcttt tgcgggtgaa tagtcatatt catcaccatc atcagagctt tcttcaattt
    28981 gcaacacccg gtcatagcgc acaccgctat ctttatctac cagttgtagc gttccatcct
    29041 cattgagcgc aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg
    29101 caatcacgtt gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga
    29161 caatctggtt gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta
    29221 tttgccgatc gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacggtacag
    29281 gctgaccgcg atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttga
    29341 tcacttcttc ccgcggccag ggcatcaggt taaacaggac gagtttgtcg gcgtcgctct
    29401 gcggcatgtt gtcggcgatt ttacgcatat agaaacgcag cagattatcc gccatgtctt
    29461 cagccagttc gaagcgggcg acgatttcgc gatgaacttt gtcactacag cagcagccga
    29521 tgctgtcgtg ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt
    29581 ggtatccaaa acccaacgtc ctggccagcg ttgccagcgg ttccagcaga ttaacaatct
    29641 tattttcaat acgtgcgtgg gcaactttga tatccatgcg cgtggaaccg atggtgcgat
    29701 gcacgcgcat atatttgcca tcaataaatt cccctttcag ggttgccaga ttatctcgct
    29761 gcgcttcgat cttctcaaat acctcttcaa agcggctcat cacaaacttt cgttgaggat
    29821 aaatttcgcg cagtttctcc atcacttcga agatattttg ctgcaatggc atctgatcat
    29881 gcccattcgg cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac
    29941 tgtcgaggcg tttgcgtaat ccgttttcgt ccgcaggtaa gtacttaccg atggcataac
    30001 ccaacggcat cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg
    30061 ttttatccgt accgtggcgc tcagaacatc cacgccagaa catggtgcag gtaatgccaa
    30121 atccattgta gatatgcggc agttgcccgg acatgccaaa ggaatcgggt aaataaccta
    30181 ttttcatcgg ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga
    30241 tggattccgc agaaacaatc gtggtatcgg tctgggtata ctaggggccg ataatcaact
    30301 tgccggcttc taccagtttc ttcacgcggt ctttgttttc cggtttcacc gcgaaataat
    30361 cttcgaggat cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca
    30421 gtcggcacag gatctcttcc atattattga ccagcagaat gcgtgactct tcggtggtga
    30481 aataccactc ccgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca
    30541 tcgtcgtttc ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac
    30601 cgcgtgacgc cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc
    30661 accaaaccag ccaattgccg ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag
    30721 cgagaaaatg ccagcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc
    30781 cgttaccata gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat
    30841 cgggatcgcc ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc
    30901 aatttcaaac tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc
    30961 cgaaaccatt ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc
    31021 gcccaggcag aatgcataag cggctttatt cactggccct ccaaggtcaa aggaacacat
    31081 aaaaccgaga atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc
    31141 ggtaagcgag ttattgatcc aggcgacagg ttcccccacc acaaacagca tcagactgcc
    31201 cgctcccaat gtaccgagca ccgggtagag ataaaaagtc aggaagccat tgaatttact
    31261 gctaagacgc aagtgatttt tcatccagcg tatcaagtaa ccggctatca atccgccaac
    31321 gaccgcgccg agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg
    31381 cgctaacgcc ggtttatctg ccagcgaata ggcggtatag gctgccagca ccggtaccat
    31441 caaaattccg agcagcccgc cgcccagttt gcggtacatc cacagccagg agttttcttc
    31501 attaaacaga tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt
    31561 cccccccgcg acaatcaacg gtacggcaaa agagatcccg ctcaacagtg cctgtttcag
    31621 ctccgttttg acgcttttca ccggttgcgt atcttgttgt aaggtacgcg tctcatcgcc
    31681 acgctcgagg gtaagcgatt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac
    31741 aggcacagaa agcgcaggaa tgccgttaaa acgctcactc tccttgatgg cgacttcagc
    31801 cgcaaaaata caggcagttg cactattgag ttgatccgcc gttaaacgcc cttcaatacc
    31861 gttagcacct tgcttttcaa cgtagacatt cacgccgagt ttgcgtccgg ctttttccag
    31921 atactccgca gccatgtagg tgtgagcaat acccgccggg caggccgtta cgcaaacgat
    31981 agttggcgcg ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga
    32041 gagcaattca tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgccag
    32101 gcgcgtggtc agcgctgtca gcagttgcat gtgcgtagta cccgcttcat tgaggggaat
    32161 cgccagcaac accactaaat caaccgcttc cgggccatcc acgccttccc actgaagcgg
    32221 ctcgctgagt gtggcgaccg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg
    32281 cacagccaac ccttcactta aggcggtcgg gccaaggctt tcacggcgat acacttcttt
    32341 cagaaattgc tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg
    32401 aatggcctct tcacggctgg taaagcgcgc attcagacac aacgcatcgc ggtgggtcag
    32461 agtcgtcagg ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg
    32521 acaaaacaat accattgcgt aatcaatttc acttgattga aattatttgc attttatttg
    32581 tattgtttac aggatgaatt tatcgggcat aatggctaaa ttcgtattaa tgagatgcta
    32641 aataatggga cacaaaccct tataccgaca gattgctgat cgcattcgtg agcaaattgc
    32701 gcgtggcgag cttaaacctg gcgatgcgtt acctaccgaa tcggcattac aaacagagtt
    32761 tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag ttagtcgagc aacagatcct
    32821 cgaaagcatt cagggcagcg ggacttacgt caaagaagag cgggtcaatt acgatatttt
    32881 tcagttaacc agttttgatg aaaaactgtc ggaccgtcac gtcgatacac acagtgaagt
    32941 tctgatattt gaagtgattc cggctgacga ttttcttcag caacagctac aaatcaccgt
    33001 gcaggatcgt gtgtggcatg tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct
    33061 ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc acctggcagg tcatggaaaa
    33121 ttcgaaatat cactttattg aagaagtgaa gaagatggtt atcgatcgta gcgaacagga
    33181 aatcattcct ctgatgccaa ccgaagagat gagccgtctg cttaatatca gccagacaaa
    33241 gccgattctt gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt ttgaatatag
    33301 ccgcaacgct tttaataccg atgactataa atttaccctg atagcgcagc gaaaatcatc
    33361 gcgataagca caaaaaggcc atcatacgat ggccaaccat gtcgaaaccg acagcccttc
    33421 gccattcctg tagagggaag cggcgagggc tatttcttat tacagatatt tatttcagaa
    33481 cagttttcag tgcttcaccg atatccgcca ggctgcgaac ggttttcacg cctgcggctt
    33541 ccagagcagc gaatttctca tccgcagtcc ctttcccacc ggcaatgatc gcacccgcgt
    33601 ggcccatacg tttgcctttc ggcgcagtca caccagcgat gtaacccaca actggcttgg
    33661 taacatgctc tttgatgtac gctgccgctt cttcttcagc gctaccgccg atctcaccga
    33721 tcatcacgat cgcttcggtc tgcggatctt tttcgaacat ttcgagaatg tcgataaagt
    33781 tagagccagg gatcgggtca ccgccgatac cgacacaggt cgactgaccg aaaccgtaat
    33841 ccgtggtctg tttaaccgct tcataggtca gtgtaccgga acgggaaacg atacccactt
    33901 tacccggttt gtgaatgtga cccggctgga taccaatttt gcattcaccc ggggtgataa
    33961 cgcctgggca gttcgggccg atcatacgaa cgcctgcttc gtccagtttc actttcacgg
    34021 tcagcatatc cagcgtcggg atgccttcag tgatggtgat aatcagtttg atgcctgcat
    34081 cgatagcttc cagaatggag tctttgcaga acggtgctgg tacgtaaata acagacgcgg
    34141 aagcgccagt agcagcaaca gcttcacgca cggtgttgaa caccggcagg ccgaggtggg
    34201 tagtgccgcc tttacctgga gttacgccac caaccatttt agtgccgtat gcaatggcct
    34261 gttctgagtg gaaagtcccc tggctaccgg taaagccctg gcagataacc ttggtgtttt
    34321 tatcaattaa aatggacatt atttcccctc cactgcggca acaacctgct gagctgcatc
    34381 cgtcagacct tttgctgcaa taatattcag gccgctgtca gccagtttct tcgcgccgag
    34441 ttcggcgttg ttaccttcca gacgtaccac gaccggtacg ttaacaccca cttccgctac
    34501 cgcgccgatg ataccatcag cgatcaggtc gcaacgaacg ataccgccga agatgttaac
    34561 cagaacggct ttcactttgt cgtcagagag gatgattttg aacgcttcgg ttacacgttc
    34621 tttggttgcg ccgccgccaa cgtccaggaa gttagccggt tcgccgccgt gcagtttaac
    34681 tatgtccatc gtacccatcg ccagacctgc gccgttaacc atacaaccga tgttaccgtc
    34741 cagcgcaacg tagttcagtt cccactgtgc agcctgtgct tcacgcggat cttcctgcga
    34801 ctggtcacgc atttcgcgca gatcaggctg gcggaacagg gcgttgccat cagcgcccag
    34861 tttgccgtcg aggcaaatca gatcgccctg tttggtgatg accagcgggt tgatttcgat
    34921 cagcgccagg tcgcgctcca ggaaaatggt cgccaggccc atgaagattt tggtgaactg
    34981 ctgaaccagt ttaccttcca gacccagttt gaacgccagc tcgcgtccct gatacggcat
    35041 cgggccagtc agcggatcaa gcgcaacttt atggatcagg tgcggagttt cttccgccac
    35101 tttttcgatt tccacgccgc cttcggtgga ggccataaag accacgcgac gggaactacg
    35161 gtctacaacg gcaccgagat acagctcttt agcgatatcg gtcgccgctt caaccagaat
    35221 ctggttaacc ggttggccat tggcatctgt ttgatacgtt accagacgct tgcccagcca
    35281 gttttctgca aaagcacgga tgtcttcttt gctgtttaca actttcacac cgcccgcttt
    35341 accgcggcca ccagcgtgaa cctgacattt cactacccac ggaccggcac cgatttttga
    35401 agcggcttct tctgcttcgc gcggagtagt acaggcataa cccaccggtg ctggtaagcc
    35461 atagcgggca aacagttgtt ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc
    35521 ttcagtaatc gttatctttt aaaccgtaga ccgacaccct atccacgtcg gtctacagtg
    35581 caggtgaagc ttaaactact acacgtccag cagcagacgc gtcggctctt ccagtaactc
    35641 tttgatcgtt accaggaagc ccacggattc gcgaccatcg atcagacggt gatcgtagga
    35701 cagcgccagg tacatcatcg gcaggatctc aacctgacca ttcaccgcca tcggacgatc
    35761 tttgatagcg tgcataccca gaattgcgct ctgcggcggg ttgatgatcg gcgtagacat
    35821 cagggaaccg aacacaccac cgttggtgat ggtgaagtta ccaccggtca gatcttcaac
    35881 cgtcagcttg ccatcacggc ctttgactgc cagctctttg attttcttct cgatgtctgc
    35941 catgccgagg gtatcgacat cacgcagaac cggcgtcacc aggccgcgcg gcgtagaaac
    36001 cgccatgctg acgtcgaaat agttgtggta aaccacgtca tcgccgtcga tagacgcgtt
    36061 cacttccggg taacgtttca gggcttcaac caccgctttc acgtagaagg acataaagcc
    36121 cagacggatg ccgtggcgtt tttcaaacgc ttcaccgtac tgcttacgca gatccataat
    36181 cggcttcatg ttgacttcgt tgaacgtggt cagcatggcg gtggagtttt tcgcttccag
    36241 cagacgctct gccacacgct tacgcaggcg agtcatcggg acacgttttt cactacgtgc
    36301 agccagagcc ggttgcgccg ccggagcagc cgctgccgga gcagactctt tcgccggggc
    36361 tttcgccaga tgtttttcca catcttcacg agtcagacga ccacccacac cggtgccttt
    36421 aatggcgctg gcgtcgagat tgtgttcagc cagcaggcga cggatcgccg ggcttaacgc
    36481 atcgttgttt tgctcttcca gagacgcctg ctggcgttgc gccggagtgg acgctttctc
    36541 ttcagatttg gcgctggttt ctttaccggc gctgttgcct tcacgcaggc gaccaaggat
    36601 ctgacgagac gttaccgttg taccttcatc ttccagaacc gcatccagaa tgccgtctac
    36661 tgatgccggt acttccagta ccactttgtc agtttcgatt tctaccagca cttcatcacg
    36721 tacgactgcg tcgccgggtt ttttatgcca ggttgcgacg gtggcatcgg ctacggattc
    36781 aggcaggtca gggaccagaa tatctacgct actcattgtg tatcctttat ttattcgacg
    36841 ttcagcgcgt cattaaccag atcttgttgc tgtttctggt gaacggacat ataccctacc
    36901 gccggagagg cggaggccgg gcggcctgca taacgcagag aagccccaaa cggaatcact
    36961 tcacggaaat gatgctggct gcagtaccat gcgccctggt tgagcggctc ttcctggcac
    37021 cagacaaaat ccttgacgtg agcaaactgc tgcaacactt cctgcatcgc tttatgcggg
    37081 aacgggtaga gttgctcgat acgcacaatg gcgacatcgt gttgattgtt cttacgacgc
    37141 tgttccagca ggtcgtaata aaccttacca gaacacatca ctacgcgctt cacgcccttc
    37201 ggatcaagct cgtcgatttc accgatggct ggcaggaagg tgccgttcgc cagttcttcg
    37261 aggctggaaa ccgccagcgg atgacgcagc agggatttcg gcgacatcac gaccagcgga
    37321 cgacgcatcc cgcgcagcgc ctgacgacgc agcatgtggt aaacctgtgc cggggtagac
    37381 ggtacgcaaa cctgcatgtt ttgctcagca caaagttgca gataacgttc cagacgcgcg
    37441 gaggagtgct ccggcccctg cccttcgtaa ccgtgcggca gcaacatcac cagaccacac
    37501 atccggcccc atttctgctc gccagaggag atgaactggt cgataaccac ctgcgcaccg
    37561 ttggcgaagt caccgaactg cgcttcccag atggtcaggg tgcgtggttc tgcggtggca
    37621 taaccatatt caaacgccag cacggcttct tctgacagta cggagtccca gacacggaac
    37681 gcgccctgac cgttatggat gtgttgcagc ggcgtgtaag tggaaccgtt agactggttg
    37741 tggatcaccg cgtggcggtg gaagaaggta ccgcgaccgg agtcttcacc cgacaggcga
    37801 accggaatgc cttcatcaac cagcgtggcg taagcgaggt tttccgcacc gccccagtcg
    37861 aacagtttct caccggctgc catcgcctgg cgatcgccat aaatcttggc aacgcgagac
    37921 tgcatttcaa ctgcttctgg taccgtactg atacgtttag ccagttcctg caggcgcttc
    37981 atctcaactt tgttcgggta ctcttcgtcc cattcgtggt tgaggtacgg cgaccaggtg
    38041 aaagagtgca tgttcatcgg acgccactct gccactacgc aatcgccagc atccagcgca
    38101 tcgcggtaca ggttaaccat ctcggtggca tcttccagcg tcgccacttt ttcctgctcc
    38161 agcttgtcag cgtagatttt gcgcggtgtc ggatgttttt tgattttctg atacatcagt
    38221 ggctgggttg cgctcggctc gtcggcttcg ttgtggccgt gacggcggta gcataccagg
    38281 tcgatgaaga catcacgttt aaaggtgtta cggaaatcga gcgccagacg ggtcacaaag
    38341 gcaacggctt ccggatcatc cgcgttaacg tggaaaatcg gtgcctgaac catcttaccg
    38401 atatcagtac agtacggtgt agaacgggca tccagcgggt tagaggtggt gaaaccaacc
    38461 tggttgttga taacgatacg taccgtaccg ccaacttcat aaccacgcgc tttcgacatg
    38521 ttcagagttt cctgaaccac gccctgcccg gtcactgcgg cgtcaccgtg gatggtgatt
    38581 ggcagcactt tgttgctgtt cggctcatca agtctgtcca gacgggcacg aacagagccg
    38641 ataacgaccg ggcttacaat ctcaaggtga gacgggttaa acgccagcgc caggtgcacc
    38701 aggccgccat cggtctggaa gtcagacgag aagcccatgt ggtatttcac gtcgcctgtg
    38761 ccgaggtgtt ctttatgttt accggcgaac tcgtcgaaca agtcttgcgg ttttttaccc
    38821 agcacgttca ccagcacgtt cagacgacca cggtgcgcca tcccgagaac cacttcgcgg
    38881 gtgccgctgt tgccagcgtg gcggatcatc tctttaagca tcgggattaa cgcgtcaccg
    38941 ccttccagcg agaagcgttt tgcgccaggg aattttgcgc cgaggtaacg ctcaaggcct
    39001 tcagcggcgg tcagttcgct taagaagcgt tttttctctt cgctattgaa agtcgcgcga
    39061 ccagactcga tacgctgttg gatccagcgt ttttcttcgg tgctggtgat gtgcatatac
    39121 tcggcaccaa tcgggccgca gtaggtttgc ttgagggcgg aaataagctc gccgagtttc
    39181 atggtttctt tgccgctggc aaatgaaccg acgttgaagg tttcctggaa gtctgcttcg
    39241 gtcagatcgt ggaaagacgg atccagatcg gccactttat cttgctgcca cagtcccagc
    39301 ggatctagat tcgcatgctg gtgaccacgg aagcggtatg cgttaatgag ctgcaggact
    39361 ttaacctgct tcacattggt gtcagggtcg gagatcgttg aagagtaacg tgaagcgtct
    39421 ttcgccaggc ggcggaaata ttcacgcgtt tgagagtgga attgatccgg tttgactccc
    39481 gtaccaggta actgctggaa cgtcgaacgc cagttagcgt caaccgagtc aggatcggtt
    39541 aagaagtctt catagagctg ttctatccag ctctggtttg cgccagagag gtaagaagag
    39601 tccaaccagg ctttcaaagc gctgttctgc atcgtgatcc cttaagcatc ttttttatgc
    39661 ttacttcgcc gtggatacta ccacgcacag tgatgtgcgt gccggggttc acttgcgagc
    39721 tcttgtctac gtagtggctc gcgaaggaac ctttaaaaac tgtcttagtg tcaggggcag
    39781 tttttaaaga tttcctgcat ttctttgtta cgcctgatgc gctgcgctta tcaggcctgc
    39841 gggtaatgac ttgtaggccg gataaggcgt tcacgccgca tccggcactg gttgcctgat
    39901 gcaacgcttg cgcgtcttat caggcctaca gtttacgcat tacgttgcaa caacatcgac
    39961 ttgatatggc cgatggcgcg cgtcgggttc agccccttcg gacatacact gacgcagttc
    40021 atgatgctgt gacagcggaa tacgctgaat gcatcgctca aaccgtcgag gcggctgtca
    40081 gtctcggtat cacggctatc gatcaggaaa cgatatgccg ctaacaagcc tgccgggccg
    40141 ataaacttat cgggattcca ccagaaagac ggacaagagg ttgaacaaca tgcgcagaga
    40201 atacattcat acagcccgtc gagtttttcg cgctgctctg gcatctgtaa atgctcgcga
    40261 gctggcggat tttgtccatt attcaacagg taaggcttaa ttttctcata ttgcgcatag
    40321 aattgtccca tgtctaccac caaatcgcgg atcaccggta aacctggcag cgggcgaatc
    40381 acaatcttct tgcccggctg gttgagtgcc gaaatcgggg taatacaggc cagaccattc
    40441 ttgccgttca tgttcagacc gtcggaaccg cacacacctt cacggcagga gcggcggaac
    40501 gacaggctgg gatctttctc tttcagctgg ataagcgcat ccagcagcat catgtcgcga
    40561 ccttcttccg cttccagggt gtaatcctgc atacgcggag catcatcaac atccgggtta
    40621 tagcgataaa ttgaaaactc gagtctcatt ttcctgtctc cgcattagta agtacgaatc
    40681 ttcggcggga atgccgggcg cagtttcggt tccatgttga cgcttcggcg cgtcatggat
    40741 tccgactcag gcagatacag ggagtggcac agccagtttt catcatcacg atccgggaag
    40801 tcgaagcggc tatgtgcgcc acggctttcg gtacggaagt tggcagaaac agccgttgca
    40861 tacgccgttt ccatcaggtt atccagttcc aggcactcaa cgcgctgggt gttgaactcg
    40921 ctggaagtgt catccagacg ggcatttttc agacgctcgc ggatcacttt caactgctca
    40981 agccctttcg ccatcgcatc accttcacgg aagaccgaga agttatgctg catacattct
    41041 tgcagcgctt tacggatcgc caccggatct tcaccgttac gattattgtt ccaccggttc
    41101 aggcgatcca gagacgcttc aacatcagac tcgctggcat cgtgcagtgc gccctgctcg
    41161 gcgatagact cttgcagatg cagacctgcc gcgcgaccaa agaccaccag gtccagcagc
    41221 gagttgccgc ccagacggtt agcgccgtgt accgatacac aagcgatttc accaacggca
    41281 aacagtcccg gaacaaccac atcttcgcct ttctcattca cagtcagcgc ctgaccggta
    41341 actttggtcg gaataccgcc catcatgtag tgacaggttg ggataaccgg aatcggctct
    41401 ttcaccggat cgacgtgagc gaaggtacgg gaaagctcca ggatacccgg cagacgggat
    41461 tcgagaactt ctttacccag gtgatcgagt ttcagtttcg cgtgtggccc ccacggacca
    41521 tcacagccgc gaccttcacg gatttcaatc atgatggaac gcgccaccac atcacggccc
    41581 gccaggtctt tggcgttcgg cgcataacgc tccataaagc gttcgccatg tttgttcagc
    41641 agataaccgc cttcaccacg gcaaccttcg gtgaccagta cgcccgcacc ggcaatgccg
    41701 gtcgggtgga attgccacat ttccatatcc tgcaccggta cgcctgcgcg gatagccatg
    41761 ccgacaccgt cgccagtgtt aatgtgggcg ttagtggtgg actgataaat acgccctgct
    41821 ccgccagtcg ccagcacggt agcgcgggct ttgaaataaa ccacttcacc ggtttcgatg
    41881 cacagtgcgg tacaacccac caccgcgcca tcctggtttt tcaccagatc cagcgcatac
    41941 cactcggaga aaatggtggt gtggtttttc aggttctgct gataaagcgt gtgcaacagt
    42001 gcgtgaccgg tacggtcagc agccgccgca gtgcgtgccg cctgctcgcc gccgaagttt
    42061 ttcgactgac cgccaaacgg acgttgatag atacgaccat catcgagacg cgagaacggc
    42121 aggcccatgt gttcgagttc cagaatcgct tccggcccgg ttttacacat atattcaatc
    42181 gcgtcctggt caccgatata gtccgaccct tttacggtgt cgtacatatg ccattcccag
    42241 ttatcttcat gggtattacc cagcgcaacg gtaatgccgc cttgcgcaga aacggtatgg
    42301 gaacgggtcg ggaagacttt agagagcagg gcacaggtct ggccgctctg ggaaatttgc
    42361 agcgccgcgc gcatacctgc gccaccggca ccaatcacaa ctgcatcaaa ttctctgact
    42421 ggcaatttca tcacacaccc cacaccacaa cgaatccata aatcacgtaa accaccagtg
    42481 caacgacaat caccagttgc agcatcaggc gcaaggccag cggtttaacg tagtcggtca
    42541 acacctgcca catgccgatc caggcatgaa tcaagataga aaacagcgcc agcagggtga
    42601 acactttggt gaacgcagag gcgaagaaac cgatccagac ttcatatgtc agttcgccac
    42661 tggtggcgaa aaaaccgacc atataaatga tgtagagcgt caggacgata gcggtagcgc
    42721 gaacgaggat gaaatcatgt acgccattgc gtcctaatgc ggaggcgttg cttaccatac
    42781 gaggactcct gcgagaagtg aaagcacgac agtaataaca aaggagattt tggcggagcg
    42841 tttacccgct tcgaatgttt cttccagata gccaaaatcc atcatcatgt ggcgaatacc
    42901 tacgacgacg tgatacgcca gagcggtaag gatgccccac atgataaatt tgacgaagaa
    42961 gctgcccata atcgcggaag cttgctcgaa accttcaggg gaagagaggc tggtacccag
    43021 aagccactgc aggatgccca ctgcaacaaa ggtgatcaca ccggaaacgc gatggagaat
    43081 ggacgctatc gccgtgatgg ggaaccggat ggtctgtagg tccagattaa caggtctttg
    43141 ttttttcaca tttcttatca tgaataacgc ccacatgctg ttcttattat tccctgggga
    43201 ctacgggcac agaggttaac tttctgttac ctggagacgt cgggatttcc ttcctccggt
    43261 ctgcttgcgg gtcagacagc gtcctttcca taactgcgcg tcatgcaaaa cactgcttcc
    43321 agatgcgaaa acgacacgtt acaacgctgg gtggctcggg attgcagggt gttccggaga
    43381 cctggcggca gtataggctg ttcacaaaat cattacaatt aacctacata tagtttgtcg
    43441 ggttttatcc tgaacagtga tccaggtcac gataacaaca tttatttaat ttttaatcat
    43501 ctaatttgac aatcattcaa caaagttgtt acaaacatta ccaggaaaag catataatgc
    43561 gtaaaagtta tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat
    43621 tgaccaattc attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa
    43681 gtacttgatt cgccatttat tcgtcatcaa tggattcttt acctgcaagc gcccagagct
    43741 ctgtacccag gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc
    43801 caggttgatg tgcgaaggca aatttaagtt ccggcagtct tacgcaataa ggcgctaagg
    43861 agaccttaaa tggctgatac aaaagcaaaa ctcaacctca acggggacac agctgttgaa
    43921 ctggatgtgc tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca
    43981 aaaggtgtgt tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt
    44041 acttttattg atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg
    44101 gcgaccgatt ctaactacct agaagtttgt tacatcctgc tgaatggtga aaaaccgact
    44161 caggaacagt atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag
    44221 attacccgtc tgttccacgc tttccgtcgc gactcacatc caatggcagt catgtgtggt
    44281 attaccggcg cgctggcggc gttctatcac gactcgctgg atgttaacaa tcctcgtcat
    44341 cgtgaaattg ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac
    44401 aagtattcca ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac
    44461 ttcctgaata tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa
    44521 cgtgctatgg accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc
    44581 accgtgcgta ccgctggctc ttcgggtgcg aacccgtttg cctgtatcgc agcaggtatt
    44641 gcttcactgt ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa
    44701 gaaattagct ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat
    44761 tctttccgcc tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc
    44821 gtaatgcgtg aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg
    44881 gaagtggcta tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa
    44941 ctgtacccga acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct
    45001 tccatgttca ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc
    45061 gaaatgcaca gttacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa
    45121 aaacgcgact ttaaaagcga tatcaagcgt taatggttga ttgctaagtt gtaaatattt
    45181 taacccgccg ttcaaatggc gggttaattt ttatatgcct aaacacaaaa aattgtaaaa
    45241 ataaaatcca ctatcggaca ttcatatatt ctaaaaaaaa atacatcggt ataaattata
    45301 aacaaataga tgatttttaa tcatctatct tcctctcagt tacatgaata aaccaccaaa
    45361 tatagcgcac ccctataatt cccaatactt tattcatcaa acatttaatt cataccatca
    45421 attaattact tttacattta ataaaattaa taatattttt aagaaaaact aaaaacattc
    45481 gacaatcaaa taagatattc ttgcaccgat atactttttt cttttctgtt aaaggactca
    45541 gttatgttta aaggacaaaa aacattggcc gcactggccg tatctctgct gttcacagca
    45601 cctgtttatg cggctgatga aggatccggt gaaattcact ttaaaggtga agttattgaa
    45661 gcaccgtgtg aaatacatca ggatgatatt gataaagagg ttgaactcgg tcaggtgacc
    45721 accagccaca ttaatcagtc acatcacagc gatgccgttg ctgtcgacct gctcttagtc
    45781 aactgtgatc tggaaaactc cagcaacggt tccggtggca agatttctaa agttgcagtc
    45841 acctttgata gctcagcgaa aaccaccggc gcagatccga ttctcaacaa caccagcacg
    45901 ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag atcaaagcaa tatcgtttta
    45961 ggcaccgcta ctccagatat cgacctggct ccgacctcca gcgaacaaac gctgaatttc
    46021 tttgcctgga tggaacaaat tgatcaggct acacctgtaa cgccaggcgc agttacggcg
    46081 aatgcaacct acgtgctgga ttataaataa gtattacttt ggcggcgcta tcgccgcctt
    46141 tcataaaata tttatcaggt atggacaccg tgaatattta tcgactctct tttgtatcct
    46201 gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt caatctgaat gttctcgata
    46261 aatcaatgcg cgaccgcatt gatatttcat tattaaagga aaaaggagtc attgctcccg
    46321 gtgagtattt tgttagcgtt gcggtaaata ataaccaaat cagtaacgag caaaagatta
    46381 actggcacaa aaatgacgat aaaaccattc cgtgcatcaa tgatttactg gtcgataaat
    46441 ttggcttaaa acctgaagtc cgtcagtcgt taccattgat aaatcagtgc gtcgatttta
    46501 gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa tcagcaactg aatattagta
    46561 ttccgcaagc ctggctggcg tggcactcag aaaactggac cccaccctcc acatggaaag
    46621 aaggtgtcgc cggtgtcctg atggattaca acttgtttgc cagcagctac cgcccacagg
    46681 atggcagcag cagcactaat cttaacgcct atggaacagc cggaatcaac acgggggcat
    46741 ggcgcttacg cagtgattat cagttgaatc atactgatag cgatgataac catgaacagt
    46801 caggcgaaat atcacgtacc tatctttttc gcccgttacc gcaattaggt tcaaaattaa
    46861 ccctcggcga aacggatttt agtcccaata ttttcgacgg tttttcttat accggcgcgg
    46921 cactggcaag tgacgatcga atgttgccat gggaactacg cggctacgcc ccacaaatta
    46981 gcggtattgc acagaccaat gccacggtga cgatcagtca atcaggccgc gtcatttacc
    47041 agaaaaaagt cccaccaggc ccatttatca ttgacgacct taatcagtct gttcagggca
    47101 cactggatgt caaagtgacg gaagaagatg gtcgggtgaa caatttccag gtttcagcag
    47161 catcgacgcc cttcctgact cgtcagggac aggttcgcta taaactggcc gcgggtcagc
    47221 cacggccctc catgtcacat caaactgaaa atgaaacctt ttttagcaat gaagtttcct
    47281 gggggatgct gtcaaacacc tcgctgtaca gcggcctgct gctttctggt gatgactacc
    47341 attctgccgc aatgggtatt gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg
    47401 tcacgtgggc cagtagccat tttgatactc agcaggacga gcggggctta agctaccgtt
    47461 ttaattacag caaacaagtg gatgccacta acagcacgat ttccctcgcc gcttatcgtt
    47521 tctccgatcg tcattttcac agctacgcca actatctgga tcacaaatac aacgacagcg
    47581 atgcgcagga cgaaaaacag acgatcagtt tatctgtggg ccaaccgatt accccactaa
    47641 acctcaatct ttacgccaac ctgctacatc aaacctggtg gaatgcagac gcctccacga
    47701 ccgccaacat cacggcaggt tttaatgttg atattggtga ctggagagat atctctattt
    47761 ctacgtcatt caatacaacc cattacgaag ataaagatcg cgacaaccag atttacctgt
    47821 cgatttcgct ccccttcggt aacggtggtc gggttggcta tgacatgcaa aacagtagcc
    47881 acagcaccac acaccgcatg tcgtggaacg atacgctgga tgaacgtaat agctggggca
    47941 tgtctgccgg actgcaatcc gaccgtcctg acaatggagc ccaggtgagc ggtaactatc
    48001 agcacctgag ttcagcgggt gagtgggata tttctggtac ctatgccgcc aatgattaca
    48061 gttccgtcag cagcagctgg agcggttctt tcaccgcaac ccaatatggt gcagcgtttc
    48121 atcgccgcag ctccaccaat gaaccacgcc tgatggtcag caccgatggc gtggcagata
    48181 ttccggttca gggcaatctc gactacacca accattttgg cattgcggtg gtgccgttga
    48241 tttccagtta ccagccttcc accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa
    48301 cagttgcaga aaacgttatc aaagaaacgt ggattgaagg cgcgataggt tacaaatcac
    48361 tggcttcccg ttccggtaaa gacgttaacg tcatcattcg caacgccagc ggtcagtttc
    48421 ctcccctcgg agcggatatc cgccaggatg acagcggcat tagcgtgggg atggttggcg
    48481 aggaaggaca tgcctggtta agcggagtcg ctgaaaatca aaagtttacc gtggtctggg
    48541 gtgatagcca gcattgctcg ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc
    48601 tgattttacc ttgtcattaa ttaaggaaat gaaaatgaca tttatgaaag gactacccct
    48661 tttgttgttg gttgccagtc tgtgcagcca cgctgcacta caacccgatc gcactcgtat
    48721 tgtgtttaac gctaatgata aagctaccag tctgcgggtg gataatcgta gcgataaact
    48781 cccctacctc gcttattcct ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct
    48841 ggtcgccctg ccgccgatac aacgtctgga accgaaagca acaacacagg tacggatagt
    48901 aaagcaagcc tcgaccacaa aattaccggg cgatcgcgaa acgctatttt tctacaatat
    48961 gcgtgaaatt cctcccgccc cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat
    49021 tcagagtcgt atcaaggtgt tctggcgacc ggctgcatta cgcaagaaag caggagaaaa
    49081 ggtcgaactg cagttacagg tcagccagca gggcaaccaa ctgacactga aaaatcctac
    49141 cgcgtattat ctgaccattg cctacctggg acgaaatgaa aaaggcgttc tccccggctt
    49201 caaaaccgtg atggttgcgc cttttagtac ggtcaacaca aacaccggaa attatagcgg
    49261 cagccagttt tatctcggtt atatggatga ttacggcgcg ttacgcatga cgacgctcaa
    49321 ctgcagcgga caatgccatt tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga
    49381 gtgttgctcg tcatctgtct gttatttctg tcgttgaagt ctgcgcttgc cctgaactgc
    49441 tactttggtt catccggtgg ctcggtagaa aaatcagaag cgattcaacc ttttgctgta
    49501 ccaggcaatg ccaaactcgg cgataagatc tgggaatctg acgatattaa aataagatct
    49561 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaactttg
    49621 aaagcgaaca tgtctacgca tgggtaaacc cctacccggg ggtgcaggat cgttattatc
    49681 aactcggagt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
    49741 acactaacca gtgtatcgac agtaagaaca ttgacattta tacccctgat gggatggcag
    49801 aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgaacctt tctcgctcgc
    49861 atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc
    49921 gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa
    49981 aacctgaaat atcatattac tggtctggaa aacattcgcg tgctggattg cagcgtcaat
    50041 ttttccattt caccggaaac acagatgatt gattttggta aatttaattt gctggatatt
    50101 cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa
    50161 tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa
    50221 gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg
    50281 ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac
    50341 gaaaaaacct atacagctga actttcgtcc attgcaggta cccccatcga ggctgggccc
    50401 ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca
    50461 gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt
    50521 attttttcct agcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag
    50581 ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc
    50641 tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg
    50701 gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc
    50761 gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct
    50821 ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat
    50881 cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg cctggggttc
    50941 ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg
    51001 cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttctggt
    51061 cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc
    51121 gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg gtacggcggg
    51181 atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct
    51241 ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga tggcgtatat
    51301 ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg
    51361 cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg caggtatggc
    51421 gtggggactg acccggttta tgcatattga ttttatgact gcctacctcg ccaccagtcc
    51481 cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat
    51541 tatggcgatg caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg
    51601 gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg gacaccagta
    51661 aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt cgcacagttc
    51721 gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac
    51781 ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg cgagtgcatc
    51841 cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa cctgccagag
    51901 gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga gcagtaatcc
    51961 tgcaaactga cggttacgaa agcgcggcga caataaccgt tcctttacca cctccggcgt
    52021 cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg
    52081 ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt
    52141 ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg
    52201 ccagacgccg taaagctgat tatggctgta gagcgttaaa ccgttgggaa aatgagttaa
    52261 caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata
    52321 agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc
    52381 ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt
    52441 tcattcttta ttaagcgggg ttatgctgcg acaacaatcc cctttttggc aaatgtagct
    52501 cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca gaccgtttgc
    52561 gccgcgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctcc
    52621 agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc
    52681 accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg
    52741 ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aaccaatgcc
    52801 ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc
    52861 tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca
    52921 atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc cggcggcagc
    52981 tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg
    53041 acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc gcctgcatga
    53101 aatccacaag caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag
    53161 ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg ctaattgttc
    53221 aaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa
    53281 atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca
    53341 ggcaatacgt ggataaccac cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc
    53401 gttatgcggc acctgcacca cgctcggtaa caaaccgtga gataacagtt cgcgatcggt
    53461 gatgcgtttt aaaatttgcc cctgtaaacg atagcccatt cggttacttt gcgagctaag
    53521 ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg
    53581 ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat
    53641 aaaatcacgc ttcgatttgc caatcgggag tcagtcacca tccttcagta aacggccttc
    53701 cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg gcggaacatc
    53761 aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa
    53821 acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcgttat catccaaccg
    53881 cgcttcgcaa cctgcacccg tcagggcaaa ccacccatca gtttcgaact caactgttaa
    53941 ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc
    54001 aatgcgcagc gcaggcatat ccagtgcgcc acagtggctg atacccgact ggcaaaaacc
    54061 gtgacgaccg ccatcctgca cagtggtgta catgcccgca cgaataatct tcagcatatt
    54121 ccctccttct gcggtacaaa tcgcacgctg tctcccggac gtaataagat gggttcgtca
    54181 cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt
    54241 gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagc
    54301 aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg
    54361 aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca
    54421 acctgttttt cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg tccgcctgca
    54481 ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc
    54541 tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa
    54601 atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga
    54661 cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc
    54721 agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct
    54781 gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc
    54841 attcagccac tcgctcaatg cgcgaatacc accgcgttca gtggcatggt gacctgcagc
    54901 ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt
    54961 aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt
    55021 gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata acggcttacg
    55081 tcccagacgc gcttcaatcc aggaggctag ttccattccc ggcacaggca tggtcagctc
    55141 tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag
    55201 ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc catacaggtt
    55261 gatatcattc gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga
    55321 ctctcctttc cagaagtagc catgatgcac aatgaccgca tctgcgccca gacgcactgc
    55381 ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt
    55441 gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc
    55501 gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct
    55561 acaccgttaa catagcgtta catcgcctgt tcgtcgatag ccaatctttt gacatattaa
    55621 gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta
    55681 tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact
    55741 ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt
    55801 attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc gctgcaaatc
    55861 tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa
    55921 ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat
    55981 gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg
    56041 atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag tgagatccat
    56101 ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg gctatggcct gtttaaatct
    56161 aacgtaagct gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt
    56221 ttctcgctga tgtatgcggc gggtaacgtg ggatctatta tcgcacctat cgcctgtggt
    56281 tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt tggcatgatc
    56341 gcgggtctgg tcattttctt atgtggcaat cgtcatttca ctcatacccg cggcgttaac
    56401 aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact ggggatggct gctggttctg
    56461 ctggtggcaa cgcctgcact gattaccgtg ctgttctgga aagagtggtc ggtatacgcc
    56521 ttaattgtcg cgactattat tggtctgggt gtactggcaa aaatttatcg caaagcagaa
    56581 aaccagaaac agcggaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg
    56641 ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga ccgcttcgtt
    56701 aaccgcgata tgtttggtta taccgttccg accgcgatgt tccagtcgat taatgccttc
    56761 gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat
    56821 cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag cgccggattc
    56881 tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc tctgccactg
    56941 atggtgttag gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg
    57001 tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg catctacatg
    57061 ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca gacatcgcag
    57121 gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt
    57181 gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat
    57241 caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc cagcaccgat
    57301 gcctgatgcg acgcttgacg cgtcttatca ggcctacgta tttcctgcaa tttattgaat
    57361 ttgcacaaat ttgtaggctg gattaggcgt tcacgccgca tccggcatct gggctcgatt
    57421 gcctgacgcg tctgttattt ccccttccgc gccgcctcat acgctgccaa cgtttgtact
    57481 ctcgcctctt tgtgctcgac tatcggttgc ggataatcca gcttcacacc tgctttctgc
    57541 gcccacttcc acggctcatg caccgctttc cctggtacat cgcgcagctc cggtaaccat
    57601 cgacgaataa actcgccctc acggtcaaat ttctcgccct gagttatcgg attgaaaata
    57661 cgaaaatacg gcgcagcatc agtacccgta gaagccgccc actgccagcc accgttatta
    57721 gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc gcgccagtcg
    57781 atcaacaaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt atgcatccag
    57841 ccagtgctgt taagctgacg catagcggca tcaataatcg ggtatcccgt tttgcctttc
    57901 tgccaggcct gtaaatgtgc gggattgctt tgccactgca cacgatccgt ccaggcaata
    57961 aatggacgat gtttacacaa cgaggggtaa tacgtcatca gatgacggta gaactcgcgc
    58021 cagatcagct cactaagcca gacactaccg gccccaccgt ccagcgcctg cggctgttcc
    58081 gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa gctggcggac
    58141 aaacggctgg tgccttccac tgccggaaaa tctcgttgtt gctcatactc tccagcaccg
    58201 ttctcgcaaa actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca
    58261 gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctt tatcgatccg
    58321 ctactacgaa cttttggtgc agcgacgcac tccggcatcc cttcccgcag ccgtttcagc
    58381 caggcattct taaaaggcgt aaagactttg tacatctcgt gattgccggt catcaccgcc
    58441 cccggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg cagcgctctt
    58501 tcaacgtcca catcccgcgc tcgctcattc acctcatact gatagttata aaaaagatga
    58561 gtaacgctgt tttccgcgca cacctgttta actatttcga cactggcgac aaagtcatcc
    58621 acttcacgga acaataaagg aatacctttt tccgccagcg cgatttgtag cccattcagt
    58681 tgagcattga taagttcagc ctgacgcggc gacatgttat gcgtctccca ctggcgtggt
    58741 gtagcgatat acagcgccag cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg
    58801 agattatcgt gcagacgtaa atcctggcga aaccaggcca gatgggtagt cataaaactc
    58861 ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc
    58921 aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt
    58981 gctcgccacg gcgataactg tcgataagcg cagccagctg ctggcgttct gtggaatcaa
    59041 tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg
    59101 cgggatgaga aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa tcatccagat
    59161 tttgccactg atgaatatca gccacaaacg ggccaagttt gcggtactcg ggctgagaat
    59221 gggccagaaa gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc
    59281 catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat
    59341 caaatcgttg tagattcatt actgctcctg aacggtatac aggagtaagt gtaggcgttg
    59401 gtaaatgatg tttgtgaagt taatccgcta gcagggcaag attacgaaag cgcgctcccc
    59461 gcaagcaact aatcaaggta gttaatcatg gctgaacacg atattacgcc agaagtcacc
    59521 atttccaaaa cacagcgccg ctataaagtc ggctatgtca gagcccgcca ccaggaccgc
    59581 aaaaccggta tagcccggta ttattcacaa catcccagcc tgcaccttaa gggcgactgg
    59641 ctgaaagagg ccggatttga taccggacgg ggtgtcacgg tgaagatttc ggaggggggc
    59701 ctgaccatca tcgctgacag tgacgaggtg caggaactgc gggatgggat gtttagtgtg
    59761 ctgaacgaaa gttaaatatt aaaaaagctg ggaagattta aaaccttcct agctttttat
    59821 tctgtcttat tataatggat actcatcatc ttccgatgaa tttataaatt gctccaagtt
    59881 tacatgaaga gtacttaacc tagtttttaa ctcattaagt gaagcgataa tttcatattt
    59941 tccaacataa tcatatgatg gatcgtattc attatctatg tcagaagcta aaaatgatga
    60001 tattcgtagt ttaaatttag cttcatcttt attacattca ttggcataag gtgaatgccc
    60061 tatcatagca tattcatcta atctaaaagg ataagatggt tttaattcat cgatcaatga
    60121 aatttcaatc catgttctat atccaggcat taatggacta atttctttga gcaaaacctt
    60181 tccacttaat aatgtattga gattgttcat tattatgtct cccacgggaa aaatatatgt
    60241 ctaacacttc caccatgttg atgaacttgt aaatgtggta aatcaccatc aggatttcct
    60301 agtccatgac ctgacactct atatgaaggt tttgttacag aaggatcttt tatatcatcc
    60361 caatgatatg aacctgcttt tgttttattt ccagtaagat attccattaa gttcttagtg
    60421 ctggggcctg actcacctgt catattttta taattatgac ctaaatatct atccataaac
    60481 aattgttcag cgtcacttct agacctaaca gtaactttgg tttcactact atctaataaa
    60541 cgattcgctt tttggttact aacagcacat ggagttaatc caagtggatc agcatatttt
    60601 agaggattag gtgcataact gtagagattt aaaccgcctc tcaaccctat cgggtcagtt
    60661 accatgaacc gcccgacatc cggatcatag tacctgtgca gattgtagtg cagccccgtt
    60721 tccctgtcga ggtactgtcc ctggtagcgc aggttctgct ccaccccgcc gtggtccttc
    60781 tcctgtatcg tgttacccca gacctgatac cccgtctccc acacgatttt cccgtcgctg
    60841 tccgtcatct cttccggcgc gccgttcaca tccgtgtgaa aatacagtat ccggttcggg
    60901 ccctctttgc ccgccttatc cacccgcgcc agcggttcat aactcccctg gtccctgtag
    60961 atgtacaggc tgcctgttcc ctccggcctg ctctcctgcg ccagccgcat tccgtcccat
    61021 aaaaattttg tccggttgta cggcttaccg tcagggctga tgtgctgttt ttccgtccgc
    61081 ctccccagtg catcgtacac ataccggtat gtcccccgcg ctgtcgtcac ttccaccatc
    61141 cggtgttccg catcataacg gtaactctgc gttccgctcc ggccctcctt ttccaccgca
    61201 cggccaaatt cgtcataacg gtagtgcaca ccacggtaag acatcagccg gttgaacggc
    61261 accacgctgt tttctgccgt ggccctttcc cccgcgcgcc tccccagcag gtttcccgcc
    61321 gcgtcgtact gccagctcgc cagatacccc tcatcctccg aacgacacgc cgtgatgcgc
    61381 cccgtggggt cgtagaagta atccgtttcc ccccgccggc tgtgccgcgt cttcagcagt
    61441 tcatcctcgc cactgtagct gtactgccgg tccgtgaacg tctccggtac caccccgcgc
    61501 atcccgctgt atgtctccct gcgcttcagc cgcccgccgg ggtcgtaaag ctgccgcgtg
    61561 ctcagtcgcc cctggcttct gagcacttca cggtgcaggt ggtcgcgcgt gaactcgctg
    61621 atgacctccc ggtccagatt tatctgctgg agatggccgc tgccgtagta cagatggcgc
    61681 tgcgttctgc cgtccggaaa ggtggtggca gtccggttgc ccagtttatc gtactggtgg
    61741 gtcaggatgc cctgcgccgt ctcctcgctc agcatccgcc ccagtctgtc gtagctgaag
    61801 cggataactt ccggcgcgtc agtttcgtgc cgctggcgac ggatttccag tagctggtct
    61861 gccgcatcat ggacgtattc cgtacggcca tcggctgtct cttttgccgt gagccggccc
    61921 gccgcatcac gcgccagccg gtggcggatt tcaccgcccc ggtccccggt gaacaccact
    61981 tccgtgaccg cgttcagcgc gttgtagcgg tacgactggc ggcagccgtc cgggtttatc
    62041 tgctccgcca gacggtccag cacatcatgg cggaaccggt agcgttctcc gttctcgttc
    62101 gtgagcgtga ccagacgccc gtaagcgtcg tacccgaatg ccgtcctgcg ccccatcgcg
    62161 tccgtccggc tgcgcacccg gccctgcccg tcccgctgcc agcgcgttat cctccccatc
    62221 gggctggtgt actttaccag cctgcccgcc gcgtctgact ggtaattcac cgtgcggccg
    62281 tccgccagta tcacctccgt cagatgaccg ctgtcgtcat acccgtagcg cgtgctgtgg
    62341 ctctgcgcat ccgtcattct gataagcctc gtgcgttcgt cgtagaacca cgccgtctgc
    62401 gagccggaac agtccgtgtg ccgcatgagc tgcccgtctt cattccactg gaggtattta
    62461 ttccccccgc gcgcatccgt tatctgcacc acctagccgt ggtggtcata ctgataccgg
    62521 gtgttctgct gcagcgggtc ggtcacggca agaaggttgc cgcgttcatc gtacacacag
    62581 cgccaggtgt ttcccgccgc gtccacctcc gtttccggct ggtgccacac cgggtgccac
    62641 tgcgcctgct ccacccgccc cagcgggtcg tgcgtctccg tgatatgccc ctgcaggtca
    62701 tacacatagc gccatttgcc gccctgaggg tcggtcatcc ccagcaacag tcgttcttcg
    62761 tcgctccagc ggaatgtcgt cacctgtccg gcttcgtcct ggtacgccgt cacctgattc
    62821 tgcgcatccc actggtgata acgtgtgcgc cccagtccgt ccgtcacgca cagcctgcgc
    62881 tgcgccaggt cccacgccag ccggtactgt tcaccgctgt ccgtgtggtg ccccgtcacc
    62941 cgccatgccg cgtcatccgg accttcatac cagccccact ggtaataata cccttcgccg
    63001 cccggctggc ggtgcagcgt catgcagtgg ttttcgtcgt aagcaaactc acgcagcagg
    63061 tgccccgtgc tgtcgtacac gccgttcagg tggccggcct cgtcatagct gtaacgacgc
    63121 agcagtaaag gtgcagtctc agggaaatgc tgataaatct gtgtcacccg ccggggatgc
    63181 gccgccagtt cgtaatacag acggatgcac ggacgctgct gttcgccgct gatttccaca
    63241 atgcggcccc ggtcgtcgta gtacagattc agacagttgc tgttacggtc gcccagcatt
    63301 ttcagccgct ggcgctgcgg gtggtgcggg tcagcctcaa acagaaaaca ctgcccgtca
    63361 tcgctgctga taagccagtg cccctgctca ccacgcctta ccgccagccc ttcaccgggg
    63421 ctgtaaaacc cgccccccgg cagtacggct tccatatcca agcgtcgccc catgcccgac
    63481 acaaaggtca tgcagttttc gtctgcgcta cccggtgtgc acacgaggct gactttatac
    63541 attacactcc agccctgacc aaacattcct tccgtacgtt cgtcacgact gctgtacacg
    63601 cgctgccagt ccagcggaaa gtggcccggc agactgaaat cgaagtcatc gtcatcggcc
    63661 aggtatttgg ccccggtggc ggcattgacc ggatgcggaa gggcactggt ggccttctgc
    63721 gtcagcatgc tcatcccgac actggcagca aaacaggcga ttttagaaag cattttgccc
    63781 gggcgaagaa acatcagcgc cacggttgcc accagggtta tctgcgacct gccgcttctg
    63841 atatccctga ccaccagtaa cggaccgcca atgcgcacat ccggtgacac aaacgccccg
    63901 ttccccggct cattcaccac cctggcctcg caggtacagc gtgccccgct gcgtgtcgcc
    63961 ggctggctgt tgatacgcac atcccgcgcg ccttccgcga tatattccgc tgcgggcggc
    64021 tcgtcatcgc ctttgaacag attactgatg ctgccaataa ccgcattcac tccctgaaag
    64081 gcggtttcca gcataccgcc cggattcagc aggttaaggg ccgttcccag ggggtcatcc
    64141 agaaaggcgg cttttttttg tgcgagaaac tgcgtcagtg aggaaggatg tttatggcag
    64201 tcgattttat cgtcaggggt cggggtggat cccggggccg cactggccac agtgggctgc
    64261 catagttccg acacaaaatg tgtcacgcca tccaccattt cagaacgccc ttcttccgta
    64321 ctgagctggt cgtgcagagc ccgggacatc agcacaggcc ctgtcaccgg gttaaccgcc
    64381 agggctatgg cccccgccat ttccagatat tccccggcca catcccggaa cgttctggca
    64441 tcctcctgac gctgcgcttc ttcctgttct gccttcgcct gcgcttcctg cgcctctatg
    64501 tcctgccgcg atgccgcagt cgccgctgcc cgcgcggcag gtttgctgtt gatccatgtg
    64561 tcaccagagc cggtgaggat gtaaccttca atc
//
LOCUS       DBKELA010000009        60820 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.009, whole genome shotgun sequence.
ACCESSION   DBKELA010000009 DBKELA010000000
VERSION     DBKELA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.009"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(110..1483)
                     /gene="argH"
                     /locus_tag="ACUQR0_000553"
     CDS             complement(110..1483)
                     /gene="argH"
                     /locus_tag="ACUQR0_000553"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HHV9107731.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1544..2320)
                     /gene="argB"
                     /locus_tag="ACUQR0_000554"
     CDS             complement(1544..2320)
                     /gene="argB"
                     /locus_tag="ACUQR0_000554"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HHV9107732.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2328..3332)
                     /gene="argC"
                     /locus_tag="ACUQR0_000555"
     CDS             complement(2328..3332)
                     /gene="argC"
                     /locus_tag="ACUQR0_000555"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HHV9107733.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3486..4637
                     /gene="argE"
                     /locus_tag="ACUQR0_000556"
     CDS             3486..4637
                     /gene="argE"
                     /locus_tag="ACUQR0_000556"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HHV9107734.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4989..7640
                     /gene="ppc"
                     /locus_tag="ACUQR0_000557"
     CDS             4989..7640
                     /gene="ppc"
                     /locus_tag="ACUQR0_000557"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HHV9107735.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7823..9556
                     /locus_tag="ACUQR0_000558"
     CDS             7823..9556
                     /locus_tag="ACUQR0_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HHV9107736.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9705..10556
                     /locus_tag="ACUQR0_000559"
     CDS             9705..10556
                     /locus_tag="ACUQR0_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9107737.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10543..10884)
                     /locus_tag="ACUQR0_000560"
     CDS             complement(10543..10884)
                     /locus_tag="ACUQR0_000560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HHV9107738.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10886..11764)
                     /locus_tag="ACUQR0_000561"
     CDS             complement(10886..11764)
                     /locus_tag="ACUQR0_000561"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HHV9107739.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11730..14027)
                     /locus_tag="ACUQR0_000562"
     CDS             complement(11730..14027)
                     /locus_tag="ACUQR0_000562"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HHV9107740.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14078..14398)
                     /locus_tag="ACUQR0_000563"
     CDS             complement(14078..14398)
                     /locus_tag="ACUQR0_000563"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HHV9107741.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14413..15492)
                     /locus_tag="ACUQR0_000564"
     CDS             complement(14413..15492)
                     /locus_tag="ACUQR0_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HHV9107742.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15801..18302
                     /gene="ptsP"
                     /locus_tag="ACUQR0_000565"
                     /pseudo
     CDS             15801..18302
                     /gene="ptsP"
                     /locus_tag="ACUQR0_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18314..18976
                     /gene="fsa"
                     /locus_tag="ACUQR0_000566"
                     /pseudo
     CDS             18314..18976
                     /gene="fsa"
                     /locus_tag="ACUQR0_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18987..20088
                     /gene="gldA"
                     /locus_tag="ACUQR0_000567"
                     /pseudo
     CDS             18987..20088
                     /gene="gldA"
                     /locus_tag="ACUQR0_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0019147 - (R)-aminopropanol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019588 - anaerobic glycerol catabolic
                     process [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20363..20980
                     /locus_tag="ACUQR0_000568"
     CDS             20363..20980
                     /locus_tag="ACUQR0_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HHV9107743.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21007..21912)
                     /gene="yijE"
                     /locus_tag="ACUQR0_000569"
     CDS             complement(21007..21912)
                     /gene="yijE"
                     /locus_tag="ACUQR0_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HHV9107744.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22006..24186)
                     /gene="katG"
                     /locus_tag="ACUQR0_000570"
     CDS             complement(22006..24186)
                     /gene="katG"
                     /locus_tag="ACUQR0_000570"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HHV9107745.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24515..25405)
                     /gene="metF"
                     /locus_tag="ACUQR0_000571"
     CDS             complement(24515..25405)
                     /gene="metF"
                     /locus_tag="ACUQR0_000571"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HHV9107746.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25481..25615
                     /locus_tag="ACUQR0_000572"
     CDS             25481..25615
                     /locus_tag="ACUQR0_000572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107747.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25754..28186)
                     /gene="metL"
                     /locus_tag="ACUQR0_000573"
     CDS             complement(25754..28186)
                     /gene="metL"
                     /locus_tag="ACUQR0_000573"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HHV9107748.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQLPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28189..29349)
                     /gene="metB"
                     /locus_tag="ACUQR0_000574"
     CDS             complement(28189..29349)
                     /gene="metB"
                     /locus_tag="ACUQR0_000574"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HHV9107749.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29626..29943
                     /gene="metJ"
                     /locus_tag="ACUQR0_000575"
     CDS             29626..29943
                     /gene="metJ"
                     /locus_tag="ACUQR0_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HHV9107750.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30127..30735
                     /locus_tag="ACUQR0_000576"
     CDS             30127..30735
                     /locus_tag="ACUQR0_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HHV9107751.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30796..31008)
                     /gene="rpmE"
                     /locus_tag="ACUQR0_000577"
     CDS             complement(30796..31008)
                     /gene="rpmE"
                     /locus_tag="ACUQR0_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HHV9107752.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31211..33409
                     /gene="priA"
                     /locus_tag="ACUQR0_000578"
     CDS             31211..33409
                     /gene="priA"
                     /locus_tag="ACUQR0_000578"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HHV9107753.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33565..34590
                     /gene="cytR"
                     /locus_tag="ACUQR0_000579"
     CDS             33565..34590
                     /gene="cytR"
                     /locus_tag="ACUQR0_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HHV9107754.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34682..35641
                     /gene="ftsN"
                     /locus_tag="ACUQR0_000580"
     CDS             34682..35641
                     /gene="ftsN"
                     /locus_tag="ACUQR0_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HHV9107755.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35734..36264
                     /gene="hslV"
                     /locus_tag="ACUQR0_000581"
     CDS             35734..36264
                     /gene="hslV"
                     /locus_tag="ACUQR0_000581"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HHV9107756.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36274..37605
                     /gene="hslU"
                     /locus_tag="ACUQR0_000582"
     CDS             36274..37605
                     /gene="hslU"
                     /locus_tag="ACUQR0_000582"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HHV9107757.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37672..38598
                     /gene="menA"
                     /locus_tag="ACUQR0_000583"
     CDS             37672..38598
                     /gene="menA"
                     /locus_tag="ACUQR0_000583"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HHV9107758.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38691..39176
                     /gene="rraA"
                     /locus_tag="ACUQR0_000584"
     CDS             38691..39176
                     /gene="rraA"
                     /locus_tag="ACUQR0_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HHV9107759.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39261..39506)
                     /gene="zapB"
                     /locus_tag="ACUQR0_000585"
     CDS             complement(39261..39506)
                     /gene="zapB"
                     /locus_tag="ACUQR0_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HHV9107760.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39932..40777
                     /gene="glpF"
                     /locus_tag="ACUQR0_000586"
                     /pseudo
     CDS             39932..40777
                     /gene="glpF"
                     /locus_tag="ACUQR0_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40817..42307
                     /gene="glpK"
                     /locus_tag="ACUQR0_000587"
     CDS             40817..42307
                     /gene="glpK"
                     /locus_tag="ACUQR0_000587"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HHV9107761.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42537..43547
                     /gene="glpX"
                     /locus_tag="ACUQR0_000588"
     CDS             42537..43547
                     /gene="glpX"
                     /locus_tag="ACUQR0_000588"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HHV9107762.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43644..44390
                     /gene="fpr"
                     /locus_tag="ACUQR0_000589"
     CDS             43644..44390
                     /gene="fpr"
                     /locus_tag="ACUQR0_000589"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HHV9107763.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44395..44823)
                     /gene="uspD"
                     /locus_tag="ACUQR0_000590"
     CDS             complement(44395..44823)
                     /gene="uspD"
                     /locus_tag="ACUQR0_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HHV9107764.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44850..45149)
                     /locus_tag="ACUQR0_000591"
     CDS             complement(44850..45149)
                     /locus_tag="ACUQR0_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HHV9107765.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45361..45801)
                     /locus_tag="ACUQR0_000592"
                     /pseudo
     CDS             complement(45361..45801)
                     /locus_tag="ACUQR0_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45902..46501
                     /locus_tag="ACUQR0_000593"
     CDS             45902..46501
                     /locus_tag="ACUQR0_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HHV9107766.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46609..47376
                     /gene="tpiA"
                     /locus_tag="ACUQR0_000594"
     CDS             46609..47376
                     /gene="tpiA"
                     /locus_tag="ACUQR0_000594"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HHV9107767.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47431..48186)
                     /gene="cdh"
                     /locus_tag="ACUQR0_000595"
     CDS             complement(47431..48186)
                     /gene="cdh"
                     /locus_tag="ACUQR0_000595"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HHV9107768.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48293..49282)
                     /gene="sbp"
                     /locus_tag="ACUQR0_000596"
     CDS             complement(48293..49282)
                     /gene="sbp"
                     /locus_tag="ACUQR0_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HHV9107769.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49602..50564)
                     /gene="pfkA"
                     /locus_tag="ACUQR0_000597"
     CDS             complement(49602..50564)
                     /gene="pfkA"
                     /locus_tag="ACUQR0_000597"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HHV9107770.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50745..51647)
                     /gene="fieF"
                     /locus_tag="ACUQR0_000598"
     CDS             complement(50745..51647)
                     /gene="fieF"
                     /locus_tag="ACUQR0_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HHV9107771.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            51934..52341
                     /locus_tag="ACUQR0_000599"
     CDS             51934..52341
                     /locus_tag="ACUQR0_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107772.1"
                     /translation="MATLKSIMAITTLIISLNAYANTNDIKTLQKQLKPWQPLEITAK
                     DSSLTVVLPGKKITSEAYNHLIMSGVCTPLWTHDAPSSFLKNVSEINIVNQYKSIGYT
                     FEDPLTTCNEIAPLMEKPAQTVVLSKTHTYTGK"
     gene            complement(52392..52610)
                     /gene="ogrK"
                     /locus_tag="ACUQR0_000600"
     CDS             complement(52392..52610)
                     /gene="ogrK"
                     /locus_tag="ACUQR0_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HHV9107773.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(52692..53855)
                     /locus_tag="ACUQR0_000601"
     CDS             complement(52692..53855)
                     /locus_tag="ACUQR0_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HHV9107774.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTSSGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEGDE"
     gene            complement(53855..54334)
                     /locus_tag="ACUQR0_000602"
     CDS             complement(53855..54334)
                     /locus_tag="ACUQR0_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9107775.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            complement(54349..56796)
                     /locus_tag="ACUQR0_000603"
     CDS             complement(54349..56796)
                     /locus_tag="ACUQR0_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HHV9107776.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASNGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIKKNKSLASTIGLIVGGALALIGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAVFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTQAATYGSG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(56789..56944)
                     /locus_tag="ACUQR0_000604"
     CDS             complement(56789..56944)
                     /locus_tag="ACUQR0_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HHV9107777.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(56941..57216)
                     /locus_tag="ACUQR0_000605"
     CDS             complement(56941..57216)
                     /locus_tag="ACUQR0_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HHV9107778.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(57273..57791)
                     /locus_tag="ACUQR0_000606"
     CDS             complement(57273..57791)
                     /locus_tag="ACUQR0_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HHV9107779.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(57804..58994)
                     /locus_tag="ACUQR0_000607"
     CDS             complement(57804..58994)
                     /locus_tag="ACUQR0_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098027 - virus tail, sheath [Evidence
                     IEA];
                     GO_process: GO:0099000 - symbiont genome ejection through
                     host cell envelope, contractile tail mechanism [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HHV9107780.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPV
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(59054..59647)
                     /locus_tag="ACUQR0_000608"
     CDS             complement(59054..59647)
                     /locus_tag="ACUQR0_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHV9107781.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSEGDTLVVWKLDRLGRSMRHLVILVEELRERGINFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTASEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
     gene            59678..60172
                     /locus_tag="ACUQR0_000609"
     CDS             59678..60172
                     /locus_tag="ACUQR0_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9107782.1"
                     /translation="MPVSRYLCIFLNVGLGEGSALPVGVPVPWPSATPPTGWIKCNGA
                     PFSAEEYPELAKVYPTNELPDLRGEFIRGWDDGRGVDTGRGILSAQGDAIRNITGWFG
                     AHATVVAKPPFVKFSAPETAENVGVGGGSTRNAVKLDLSLSVPTASENRPRSIAFNYI
                     VRAA"
     gene            60172..60774
                     /locus_tag="ACUQR0_000610"
     CDS             60172..60774
                     /locus_tag="ACUQR0_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9107783.1"
                     /translation="MTQAKLNSEFIATVAGDMTVYNYDNTTREYISSSTEYLAVGVGI
                     PACSCLDAPVTHKAGYAICRSADFNSWEYVPDHRGEIIYSTETGESKEITALGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLRAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(60746..>60820)
                     /locus_tag="ACUQR0_000611"
     CDS             complement(60746..>60820)
                     /locus_tag="ACUQR0_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9107784.1"
                     /translation="LLMRVNTSTALDIEWPTPPAVQAR"
BASE COUNT        14661 a        16620 c        15462 g        14077 t
ORIGIN      
        1 tgctgacaaa tttctgcctg atgcggcgta aacgccttat ccgcctacga agtgtcgcac
       61 tttgtaggcg tggtaaactc cccattttca ggggagcaat ataaacaaat tactctaacc
      121 gagcctgcgc aaaagcaatc gcctgcgcca cctgctgcgg tgagacgccg ccttttgccg
      181 cacgcttttc gaggcacgat tgtagcgaca gaatcggata gacatcttcg ccaatcacct
      241 gactgaattt ctgcaactca ctgagtggca gatcttccag cggtttgccc tgacgaatgg
      301 cttccaccac cgcttcaccc acaatatggt gcgcctcgcg gaaaggtacg cctttcgcca
      361 ccaggtaatc cgccagttca gtggcgttcg catacccctg ctgcgccgct tcctgacaac
      421 gcggacgttt cacctgaatg ccgtccagta ccagcgccgc catatgcagg cagtccagcc
      481 aggtatcgag cgcgtcgaac agaccttctt tgtcttcctg catatctttg ttgtaagcca
      541 gcggcaaacc tttcagcgtc atcatcatgc cggttaacgc accctgcacc cggccgcatt
      601 taccgcgaat cagctccagc gcatccgggt ttttcttctg cggcattaat gatgaaccgg
      661 aagtcacgcg gtccgaaagc tccacaaacc ccgcttcgcc ggtgttaaag aaaatcagat
      721 cttcagcaaa acgcgacaga tgcaccatgc cgatagcggc agcagaaagc agttccaaca
      781 catggtcacg gtcagaaacg ctgtccagac tgttacgggt cgccgaagca aagcccagcc
      841 agcctgctaa ctgttcacgg tcgatttcat aggccgttcc cgccagcgcg ccacagccta
      901 atgggctgac atccagacgc ttaagcgcat cctgcaaacg gctttcatca cgcgccagca
      961 tctcaacata ggccaggcac cagtgcgcga acgtcaccgg ctgggcgcgt tgcaggtgag
     1021 tgtaacctgg cattaccgcg tcctgattgt tttgtgcggt ttccaccagc gccgattgca
     1081 gctgccggtt agccgtcagt aactcgctaa cggtatcttt gcaccacagt ttcaggtcag
     1141 tcgctacctg atcattacgg ctacgcccgg tatgcagctt tttgcctaac tggcccactt
     1201 tgtcgatcag tttgccttcc acccagctat ggatatcttc ggcgtcgctt tcaaggattt
     1261 gttgtggcct ggcgcgaaca tcttccagca acacgttcag cgcctcttcc agttgcgcct
     1321 gctcttctgc agttaacacg ccgaccgtta ccaaggcttt ggaccaggcc acagagccaa
     1381 caatatcctg ctccgccaga cggtaatcaa agcgcagtga gtcgttgaat tgtttgaacc
     1441 gttgatctgc tgcctgggta aaacgcccgc cccaaagtgc cataactctg tttccttatt
     1501 ttgaaattca atgccggaaa gctgcgcctc cggctaacaa aacttaagct aaaatccgcg
     1561 tacccatcgg cataccgtta aacagtgccg gaagctgctc cgcataacgc caagaagcga
     1621 tatctaccgg acggcccagc gtgcgggccg catccagcgc cgcgttcact ttcactatca
     1681 tgccgtcagt aataatgccc tgctcaatca gttgttctgc tttcgcggcg gtcatttcgg
     1741 caatgcgttg ccctttgccg tcgagaatgc cgctgacgtc ggagagcaaa atcagatccg
     1801 cgcccagcgt tgccgccagc gccgttgccg cctggtcggc attgacgttc atcagttgcc
     1861 cttcgtctgt tacgccaatg gagctgacca ccggcagata accgttctcc agcaaggagt
     1921 tgataagctt aggcgaacct ggctgcgcca gtccaacatg acctaactct tcatcaagct
     1981 gggtcacttt gacgctgtcg ccgtcaccga gaaacaaacc tacggccgca atctgatgtt
     2041 tcttcgccca tgccaacagg gttttatttg ccgttcccgc cagtgctccg gtgataatgt
     2101 ctatctgatc agcaggcgtc acccgcaggc cgtttttctt tttcaccggc agattcagcc
     2161 ctttcatcag ctcatccacc acgcaaccgc cgccgtgcac aatcaccagc ggacgctgat
     2221 gtgactcacg ataattcacc aatgcgctaa acagacgttc cagcgcctct tcactatcca
     2281 gcagtacgcc gcccagtttg ataattaatg gattcatcat tgcaccctta aataagagac
     2341 tgcgtttccg catagccgaa acgaatattg gcgcactgta ccgcttgtgc cgccgcgcct
     2401 ttcagtaagt tgtcttcggt cgccacaata atcagatgct cgccctgaac ggcaaacccg
     2461 atatcgcaaa atggcagccc aacgacattt ttcagcgccg gaacgccttt gtcatacagc
     2521 cgcaccagcg gtttatgggc atacgcctgc tgtaacgctt gcgcgacttg cgcctgggtc
     2581 acacccgatt tcaggcggca ggtaatggtt tcgagaatgc cgcgcgggaa attgcccaga
     2641 tgtggggtga agataacgtc agcaccgagg tgtgtggcga tctctggttg atggcgatga
     2701 gtaaagacgc catacggttg caggctaact tcacaaaaac tatttgaaat cgctgcctta
     2761 cgtcctgcac cgctcacgcc gctggtggcg ttgatcaccg gccactgatt gaggtcaaga
     2821 agatcggcat caatcaacgg tttcagcgcc agctgtgccg ccgtcggata acagcctggc
     2881 accgcaatca aattcgcttc ttttaattta ttaccgcacc actccgccag accgtaggct
     2941 gcctgttcca gcagttccgg gtattgatgg gtaaagccgt aatatttttc atagaaggtg
     3001 gcgtcgttaa cacgaaacgc gccggaaagg tcgaacacca cgcagcccgc ttcaagaaac
     3061 tgcggcgcta aatcgtggct aacttcatgg gcggtggcga gaaacactac gtccacccct
     3121 gggctaaact cgctgatatc cgacatcggc tgcaacggca gctcaacgat gccttttagc
     3181 tgcggatgca aatcggagat taactttccc gcatcattgc tttgcgctga aacagtcaaa
     3241 gcggttatgt tcatatgcgg atggcgattt acataggtca ctagctctgc gccagcgtag
     3301 ccgctggcac ccacaatcag cgtattcaac atcggggcta ttcaccttct tatgtctggt
     3361 tgccaggtta aacgtaaaac attcacctta cggctggtgg gttttattac gctcaacgtt
     3421 agtgtatttt tattcataaa tactgcatga atattgatac tatcatgacc agaggtgtgt
     3481 caacaatgaa aaacaaatta ccgccattta tcgagattta ccgcgctctg attgccacac
     3541 cttcaataag cgctacggaa gaggcactcg atcaaagcaa tgcagattta atcactctgc
     3601 tggcggactg gtttaaagat ttgggcttca atgtggaagt gcagcctgtt ccaggaactc
     3661 gcaacaaatt taatatgctg gccagttacg gacagggggc tggcggcttg ttgctggcgg
     3721 ggcataccga tacggtgcca tttgatgacg gtcgctggac gcgcgatccg tttacactga
     3781 cggagcatga cggcaagctt tacggcttag gcaccgccga catgaaaggc ttttttgcgt
     3841 ttatccttga tgcgctacgc gatgtcgacg tcacgaaact gaaaaaaccg ctctacattc
     3901 tggcgactgc tgatgaagaa accagtatgg ccggagcgcg ttattttgcc gaaactaccg
     3961 ccctgcgccc ggattgcgcc atcattggcg aaccgacgtc actacaaccg gtacgcgcac
     4021 ataaaggtca tatctctaac gccatccgta ttcagggcca gtcggggcac tccagcgatc
     4081 cagcacgcgg aattaacgct atcgaactaa tgcacgacgc catcgggcat attttgcaat
     4141 tgcgcgataa cctgaaagaa cgttatcact acgaagcatt taccgtgcca taccctacgc
     4201 tcaacctcgg gcatattcac ggtggcgacg cttctaaccg tatttgcgct tgctgtgagt
     4261 tgcatatgga tattcgtccg cagcctggca tgacactcaa tgaacttaat ggtttgctca
     4321 acgatgcatt ggctccggtg agcgaacgct ggccgggtcg tctgacggtc gacgagctgc
     4381 atccgccgat ccctggctat gaatgcccac cgaatcatca actggttgaa gtggttgaga
     4441 aattgctcgg agcaaaaacc gaagtggtga attactgtac cgaagcgccg tttattcaaa
     4501 cgttatgccc gacgctggtg ctcggtccgg gttccatcaa tcaagctcat cagcctgatg
     4561 aatatctgga gacgcggttt attaaaccca cccgtgagtt gattatccag gtaattcacc
     4621 atttttgctg gcattaaaac gtaggccgga taaggcgctc gcgccgcatc cggcaccgtt
     4681 gccaaactcc ggtgccgcaa taatgtcgga tgcgatgctt gcgcatctta tccgacctac
     4741 acctttggtg ttatgtatgg cgatttttta acatttccat aagttacgct tatttaaagc
     4801 gtcgtgaatt taatggcgta aattcctgct atttattcgt ttgctgaagc gatttcgcag
     4861 catttgacgt caccgctttt acgtggcttt ataaaagacg acgaaaagca aagcccgagc
     4921 atattcgcgc caatgcgacg tgaaggatac agggctatca aacgataaga tggggtgtct
     4981 ggggtaatat gaacgaacaa tattccgcat tgcgtagtaa tgtcagtatg ctcggcaaag
     5041 tgctgggaga aaccatcaag gatgcgttgg gagaacacat tcttgaacgc gtagaaacta
     5101 tccgtaagtt gtcgaaatct tcacgcgctg gcaatgatgc taaccgccag gagttgctca
     5161 ccaccttaca aaatttgtcg aacgacgagc tgctgcccgt tgcgcgtgcg tttagtcagt
     5221 tcctgaacct ggccaacacc gccgagcaat accacagcat ttcgccgaaa ggcgaagctg
     5281 ccagcaaccc ggaagtgatc gcccgcaccc tgcgtaaact gaaaaaccag ccggaactga
     5341 gcgaagacac catcaaaaaa gcagtggaat cgctgtcgct ggaactggtc ctcacggctc
     5401 acccaaccga aattacccgt cgtacactga tccacaaaat ggtggaagtg aacgcctgtt
     5461 taaaacagct cgataacaaa gatatcgctg actacgaacg caaccagctg atgcgtcgcc
     5521 tgcgccagtt gatcgcccag tcatggcata ccgatgaaat ccgtaagctg cgtccaagcc
     5581 cggtagatga agccaaatgg ggctttgccg tagtagaaaa cagcctgtgg caaggcgtac
     5641 cgaattacct gcgcgaactg aacgaacaac tggaagagaa tctcggctac aaactgcccg
     5701 tcgaatttgt tccggtacgt tttacctcgt ggatgggcgg tgaccgcgac ggcaacccga
     5761 acgtcactgc cgatatcacc cgccacgtcc tgctgctcag ccgctggaaa gccaccgatc
     5821 tgttcctgaa agatattcag gttctggttt ctgaactgtc gatggttgaa gcgacccctg
     5881 aactgctggc gctggttggc gaagaaggtg ccgcagaacc gtatcgctat ctgatgaaaa
     5941 acctgcgttc tcgcctgatg gcgacacagg catggctgga agcgcgcctg aaaggcgaag
     6001 aactgccaaa accagaaggg ctgctgacac aaaacgaaga actgtgggaa ccgctctacg
     6061 cttgctacca gtcacttcag gcgtgtggca tgggtattat cgccaacggc gatctgctcg
     6121 acaccctgcg ccgcgtgaaa tgtttcggcg taccgctggt ccgtattgat atccgtcagg
     6181 agagcacccg ccataccgaa gcgctgggcg agctgacccg ctacctcggt atcggcgact
     6241 acgaaagctg gtcagaggcc gacaaacagg cgttcctgat ccgcgaactg aactccaaac
     6301 gtccgcttct gccgcgcaac tggcaaccaa gcgccgaaac gcgcgaagtg ctcgatacct
     6361 gccaggtgat tgccgaagcg ccgcaaggct ccattgccgc ctacgtgatc tcgatggcga
     6421 aaacgccgtc cgacgtactg gctgtccacc tgctgctgaa agaagcgggt atcgggtttg
     6481 cgatgccggt tgctccgctg tttgaaaccc tcgatgacct gaacaacgcc aacgatgtca
     6541 tgacccagct gctcaatatc gactggtatc gcggcctgat tcagggcaaa cagatggtga
     6601 tgattggcta ttccgactca gcaaaagatg cgggcgtgat ggcagcttcc tgggcgcaat
     6661 atcaggcaca ggatgcatta atcaaaacct gcgaaaaagc gggtattgag ctgacgttgt
     6721 tccacggtcg cggcggttcc attggtcgcg gcggcgcacc tgcccatgcg gcgctgctgt
     6781 cacaaccgcc aggaagcctg aaaggtggcc tgcgcgtgac tgaacagggc gagatgatcc
     6841 gctttaaata tggtctgcca gaaatcaccg tcagcagcct gtcgctttac accggggcga
     6901 ttctggaagc caacctgctg ccaccacctg agccgaaaga gagctggcgt cgcattatgg
     6961 atgaactgtc agtcatctcc tgcgatctct accgtggcta cgtacgtgaa aacaaagatt
     7021 ttgtgcctta cttccgctcc gctacgccgg aacaagaatt gggcaaactg ccgttgggtt
     7081 cacgtccggc gaaacgtcgc ccaaccggcg gcgtcgagtc gctgcgcgcc ataccgtgga
     7141 tttttgcctg gacgcaaaac cgcctgatgc tccccgcctg gctgggtgca ggtacggcgc
     7201 tgcaaaaagt ggtcgaagat ggcaaacaga gcgagctgga agccatgtgc cgcgattggc
     7261 cattcttctc gacgcgtctc ggcatgctgg agatggtctt cgccaaagca gacctgtggt
     7321 tggcggaata ctatgatcaa cgtctggtag acaaagcact gtggccgtta ggtaaagagt
     7381 tacgcaacct gcaagaagaa gacatcaaag tggtgctggc gattgccaac gattcccatc
     7441 tgatggccga tctgccgtgg attgcagagt ctattcagct acggaatatt tacaccgacc
     7501 cgctgaacgt attgcaggcc gagttgctgc accgctcccg ccaggcagaa aaagaaggcc
     7561 aggaaccgga tcctcgcgtc gaacaagcgt taatggtcac tattgccggg attgcggcag
     7621 gtatgcgtaa taccggctaa tcttcctctt ctgcaaaccc tcgtgctttt gcgcgagggt
     7681 tttctgaaat acttctgttc taacaccctc gttttcaata tatttctgtc tgcattttat
     7741 tcaaattctg aatatacctt cagatatcct taaggaattg tcgttacatt cggcgatatt
     7801 tttttcaaga caggttctta ctatgcattc cacagaagtc caggctaaac ccctttctag
     7861 ctggaaagcc ctgggttggg cactgctcta cttttggttt ttctctactc tgctacaggc
     7921 cattatttac atcagtggtt atagtggcac caacggcatt cgcgactcgc tgttattcag
     7981 ctcgctgtgg ttgatcccgg tattcctctt tccgaagcgg attaaaatta ttgccgcagt
     8041 gatcggcgtg gtgctatgga cggcctctct ggcggcgctg tgctactacg tcatctacgg
     8101 gcaggagttc tcgcagagcg ttctgtttgt gatgttcgaa accaacacca acgaagccag
     8161 cgagtattta agccagtatt tcagcctgaa aattgtgctt atcgcgctgg cctatacggc
     8221 ggtggcagtt ctgctgtgga cacgcctgcg cccggtctat attccaaagc cgtggcgtta
     8281 tgttgtctct tttgccctgc tttatggctt gattctgcat ccgatcgcca tgaatacgtt
     8341 tatcaaaaac aagccgtttg agaaaacgtt ggataaccta gcctcgcgta tggagcctgc
     8401 cgcaccgtgg caattcctga ccggctatta tcagtatcgt cagcaactaa actcgctaac
     8461 aaagttactg aatgaaaata atgccttgcc gccactggct aatttcaaag atgaatcggg
     8521 taacgaaccg cgcactttag tgctggtgat tggcgagtcg acccagcgcg gacgcatgag
     8581 tctgtacggt tatccgcgtg aaaccacgcc ggagctggat gcgctgcata aaaccgatcc
     8641 gaatctgacc gtgtttaata acgtagttac gtctcgtccg tacaccattg aaatcctgca
     8701 acaggcgctg acctttgcca atgaaaagaa cccggatctg tatctgacgc agccgtcgct
     8761 gatgaacatg atgaaacagg cgggttataa aaccttctgg atcaccaacc agcagacgat
     8821 gaccgcccgc aataccatgc tgacggtatt ttcgcgccag accgacaagc agtactacat
     8881 gaaccagcaa cgtacgcaga gtgcgcgtga atacgacacc aacgtgctga agccgttcca
     8941 ggatgtgctg aatgaccctg cgccgaagaa actgatcatc gttcatctgc tgggtacgca
     9001 tatcaaatac aaataccgct acccggaaaa tcagggcaag tttgatggca ataccgatca
     9061 tgttccgcca ggattaagcg cagaagagct ggaatcatat aacgattatg acaacgctaa
     9121 cctgtataac gatcatgtgg ttgccagcct gattaaagac tttaaagcgg cagacccgaa
     9181 cggattcctg gtttacttct ctgaccacgg tgaagaggtt tacgacacgc cgccgcacaa
     9241 aactcagggg cgtaacgaag acaacccgac gcgccacatg tacaccattc cgttcctgct
     9301 gtggacgtcg gaaaaatggc aagcgactca tccccgtgat ttctcacagg atgtcgatcg
     9361 taaatacagc ctggcggaac tgatccacac ctggtcagat ttggcgggct tatcttacga
     9421 cggttacgat ccaacccgtt cagtggtgaa tccgcagttc aaagaaacta cccgctggat
     9481 tggtaacccg tacaagaaaa acgcgctgat cgattacgac actctgccgt atggcgatca
     9541 ggtgggtaat cagtagatga cctttgtatc gcgtctggcg ctatttgccg gatgcgatac
     9601 tgccgcaatg tttacatcga actcccctca ggccacactt ttcaggtggc cttttttaat
     9661 aatgccagtt actgttttcc cgtctacata cctaagggaa taacatgtat cacgacgtca
     9721 gctatctgct ctcccgcctg atcaacggcc cgctgtccct gcgccagatt tactttgcca
     9781 gcagtaacgg cccggtgccc gatcttgcgt atcaggtcga ttttccacgg ctggaaattg
     9841 tgctggaagg tgagtttgtt gataccggcg ctggagcaac gttagttccc ggcgatgtgc
     9901 tgtacgttgc cgctggtggc tggaatttcc cacaatggaa aacacccgct accaccttta
     9961 gcgtgctgtt tggcaaacag caactcggct tcagcgtcgt gcaatgggat ggcaaacaat
    10021 atcaaaatct ggcgaagcaa cacgtcgccc gacgcggccc acgcataggt tcttttctgc
    10081 tacaaacgct caatgaaatg caaatgcagc cgcaggagca gcaaacggca aggcttatcg
    10141 tagccagcct gcttagccac tgccgcgatt tgctcggcag ccaaatccag accgcctcac
    10201 gcagccaggc actatttgaa gctattcgcg attatatcga cgaacgctat gcctccgcgc
    10261 ttacccgcga atctgtcgca caggcattct atatttcacc aaactatctc tcgcacctgt
    10321 ttcaaaaaac gggggccatt ggttttaacg aatacctgaa tcacacgcga ctggagcacg
    10381 ctaagacgtt attgaaaggc tatgacctga aggtaaaaga agtggcgcac gcctgcggct
    10441 ttgtcgacag caactacttc tgccgtctgt ttcgtaaaaa caccgaacac tcgccgtcgg
    10501 aatatcgccg gcagtaccac agccaactga cagaaaaacc gactactcca gaataagatg
    10561 ggtttgctgc ggcgcagaaa gcactttgcg caccgcgctc attacccgct gcggctcacg
    10621 caggaatgcg tagatgctgc attgcacata gcggcaatgt tcaaatcgct cggcaccggc
    10681 aagctcgata tccgtaatca acagagcaac atccgcccga cggatatcct cgtccgctaa
    10741 acgattctcc gttcccagcg cgccctgagt ttcaatgctg acgccccact tctctaactg
    10801 gcacaacttt tccagccgtt ccgccgccat ataagtatgc gccacgccgc ttacgcaggc
    10861 ggttactgcc accaggtatg ccattttaac ctcccacggt aacctgaaat ccggcccgtt
    10921 ctgccatttc gcgcatcgtt gtcacatcgg ctgacgacgg cgcaggcacc tctttcatcg
    10981 accatgtttt ccccagcagg cggtatttcg gttcgccgta ctgatgaaac ggtaacagat
    11041 ggatctgcct gattttcagc gggatcagta catccagcgc ctgctgcata ttctcccggc
    11101 tgagcgtgaa accagggatc aacggtaaac gcgggatcac gttgacaccc tcactcacca
    11161 gcaaacgcag attctccagc acgcgtggca ggttcatctt caccacatcc cgcgcctgag
    11221 tcgcgtccat aatttttaaa tcgaacagca cttcatcgca caatttcgcc agcggtaaca
    11281 gcttgcttgc tggcgcgtct ccggcagttt caatggcgca tgagacaccc cacagccgca
    11341 gtcgctgtaa aaaacgggta gcaaactccg cctgcattaa cacttcgccg ccagaaagcg
    11401 tgacgccgcc gccggacgtg cgaaagaaaa tgtcatcttt catcacttcc cgttccagag
    11461 cgtcaaggct gatatcgcga ccaatccgtt caaacgcccc ggaggggcat tcatccgcat
    11521 cacgcaaaca tttcgcacag tgcagacatt tcgcctctct gcgtaccgtc tggattttgc
    11581 cggagatcga ctccggatta gcacaccacg ggcaaagatg cggacagcct ttaaaaaaga
    11641 ccaccgtacg aatgccctcg ccgtcattca gtgaataacg ctgaatgttg aaaatgcgcg
    11701 ccacatcatc gcggcgcgtt tccacaacgt tacagctgat gcgctgtccg gcggatgatg
    11761 tcatcctgga tctccttcga cagttcgaca aagaaggcgc tgtatccggc aacgcgcacc
    11821 accagcccgg cataatcttg tgggcgctgt tgcgcttccc gcaacgtgtc ggcgttcacc
    11881 acgttaaact gaatatgctg caacttaagc tgggtaaaca cccgtaagaa gtcggccagt
    11941 ttgcgtaatc ccgcttcacc ttccagggtc gccggagtga atttcacgtt cagcaacgta
    12001 ccgttagaca gcagcgtatt atcgagctta ctgactgact tcagtaccgc cgttggccct
    12061 tgtgcgtcct gacccagcat aggtgacaag ccgccgtctg ccagttgttc cccggcaaaa
    12121 cgaccgtctg gcgtcgcgcc aaccaccgat cccaacggaa cgtgagcaga aacggtatac
    12181 gatcccggcg tgaagtaacc gccgcgcggg ttctggtatt tttccacttc tttgcagtag
    12241 tggcgcaaca gttcggcgct aatgttatcc acctcgtcga tatcgttacc gtatttctca
    12301 aagcggttaa ttaagcgagc gcggactttt tcgccttctg gcgttgcaaa gttggctttt
    12361 aataccgaca gcaattcgtc aaaacttaaa cgctgttgct caaaaaccat ccctttcaac
    12421 gcatggagag aatcgctcag gttggcgata ccaattccct gtacgccgga gaagttataa
    12481 cgcgcgccgc catcggtaat atcgcgacct ttttccagac aatcgctgat aaaagacgag
    12541 agcagcggta caggtgccca gtcgcgatgg ccgatatcac aaatattgct gccctcaacc
    12601 atcagggtga tgtagtggct gatcttggcg cggatctgct ccagtaaacc ttcataagtc
    12661 agcgcagcat tgccttcatt ttcatgcagg cagatttcca tcactttcag cagattaaac
    12721 atcgcgatgt catgcaagcc gtaggttctg ccgggaatag ataattccac acagcccact
    12781 acggaatagt cgcgcgcatc ttccagcgaa acgccacggt tgaggaacgc tggcaccacc
    12841 acttcatcgt taaagatttg cggaataccg gtgccgaggc gaatggtttc ggcggttttc
    12901 atcaggaacg gcgtgtcgat caaggcgtta gtgcgcacgc cgaggttcgg ttgcggtaat
    12961 tgcacgcttt gataggtgtc aaggcaaagg aacgaaagca cgttcaccgc gctacgtccg
    13021 ttctcggtta acccgccgag cagtgcggta tagccggtcg ggaaaccggc gaaataacgc
    13081 gcgctactgg tggagcgcaa cagcacgatg tcgttgcatt tcacccataa agattcgagc
    13141 agttctttca ggaacgccgg atcttcgccc tgggttaaag atgcctgata gaacggcaac
    13201 atatactggt cgaagcgccc taaagacagc gaactggcgt tggattcgta ttgcagaatg
    13261 atgttcatgt accagaataa ctggcacgcc tgccagaacg tctgcggctt atgttgcgcg
    13321 ttatggcggg agatctccgc aatagtcagc agctcttcgc gacgctgggc atctgtgcag
    13381 tttgccgcca tcgtttccgc cagttcggcg taacgcaaaa tgtgtttctg cgaggcttcc
    13441 agcagtaaca acgcggcctg ataaaagtga ttctccggct gttgctgaca atgttgctgc
    13501 atctgtgcca ccagctcacc cagaccgtga ttcaacaggc gtgggtaatc aataataatg
    13561 tgcccctggc ctttatccgt ctggttgatg ctgaaaatct gcgtgttggt cgcggctttt
    13621 acttcatctg tcatctgccc gttgatgaaa tctttcatcg aacgtttttc ccagtacggg
    13681 aacaactctt cacggtagat acgtttatct tcttcgctga tagcaaagcg gtcctgcgga
    13741 cgcgtcggga attgatccag ctctttcagc agccagtaag ggtccatttc cggcgacata
    13801 atcccggcgc gcggttttac ggtgcggtta ccggcaatca gttcttcatc acgaatcgaa
    13861 atttcaacat gttcaaggat atacgctgtt gctttcgccc ggcgcaatat caccggttcg
    13921 ccttcggttt gccgatggct ggcggtataa agcagcgccc gctccagcga gatttcacgg
    13981 gtattggcaa acagtgcagt tttgaggcga gagatacgat tcgtcatgag gaactcctgt
    14041 gggaaagcga acccgccctt gcgagcggga gaagaactta agcggtttgc gccaggtgag
    14101 cttcaatttt gctcataatg gcgtcagcac gttttactgc atcgctgatg ttgacgcgca
    14161 cgatggtctt gccagcaaaa cgctcttcaa acttgatgcc gatatctttg gtcagaatca
    14221 ccatatcagc gctcgccacg tcttccgcag tcagttcatt ttccagacca attgacccct
    14281 gggtttctac tttcacttcc cagcctttcg ctttcgcggc actttccagc gcctctgctg
    14341 ccatataggt atgggcaaca cctgaaggac atgcggttac tgcaataatt ttcgtcatgg
    14401 ctcgttcctc aattaattaa tttcaaaatc caaatccagg tcgtcttctt tttcatcagt
    14461 cgaagaccca tttttacgcg ccagactttt cagcacgttc acacaaacag ccgtcaccac
    14521 tgctccgaca gccactgctg cgatataacc caacttacct tcaaccaccg gcagcacaat
    14581 cagaccaccc cagcctgcat agcactgcgc accgaccagc gccgcagtta ctgcaccaca
    14641 aactgaaccg accataatgg aaggaatgac acgcagcgga tcggcagcgg cgaaaggaat
    14701 cgccccttca gtaacgccaa cacagcccat caccagtgcc gctttgccgg tttcgcgctc
    14761 ttctgcggag aaatttttac gaccaatcag cgtcgccagc cccatcccca gcggcgggat
    14821 acaaataccc acggcagcaa tggccaccac ggtataaaca ccctgagcaa cgcaaatcag
    14881 catgaaggca taggctactt tgttaaccgg accgcccata tcgaacgcca gcatcagccc
    14941 catgatcacc gccagcataa caatgctgcc ctgctgcatc ccctgaagcc actgagtcag
    15001 gctgttggtc aacgccccta caggctcgcc caagccccac atcatgatgc ctgcggtaat
    15061 cagtgtgccg atgataggaa taatgaagat gggcatcacc gagcgcagaa ctttatgcac
    15121 cggaattttc ttcaggtaat gcaccacgat gccgccgata atcccggcga tcagcgcacc
    15181 aaagaagccc gcaccaaagc tgttaccaac ccaggcaccg atagcgcacg gagccagcgc
    15241 agaacgctct gcaatggagt aaccaatgta agcagcgagg aaaggcacca tcagcgtcaa
    15301 acctgcaacg ccgatatcaa acagtttttt caaattcgga tcggctacgg catccggcac
    15361 tgcgcctttg ccatacaaca tgacggaaac cgccagcaaa ataccgcccg ataccacgaa
    15421 gggaatcatg tgtgaaacgc ccgtcattaa atgctgacgg gtatttttca ggatctgcac
    15481 caactcattc ataagctgct cccgggcttg caaaagccca tgtccatatt tttgatggag
    15541 gaaacaatag gcaatcgcag gcggagcaaa cagtggataa aaaggccatt tactggactt
    15601 ttgtaatatc agtacaaaaa tgcgatccgc ctcataactt gcgataatcc ggaagcatgc
    15661 cgcaaaacgg cgtcttgtga gacgcgtcgc aaattgacgg ttacattaca tttgtccagc
    15721 ttttggcaga tttgtcacat ggcaggccgt taccgtgcgc tcttatcctg taaacagaaa
    15781 accgtaccgg gagaaaggca atggccctga ttgtggaatt tatttgtgag ctacctaacg
    15841 gcgtacatgc gcgtccggca agccacgttg aaacgctgtg taatactttt tcatctcaaa
    15901 ttgagtggca taacctgcgt actgaccgta aaggcaacgc caaaagcgct ctggcgctga
    15961 ttggcaccga tacgctggtg ggtgataact gccagctact gatttccggg gccgacgaac
    16021 aggaagggca ccagcgttta agccaatggc tgcgcgatga attcccccac tgcgatgcgc
    16081 cgctggcgga agttaaatct gacgaactgg aaccactgcc ggtttcactg accaatctga
    16141 atccgcaaat tatccgcgcc cgcaccgtgt gcagcggtag tgcaggcggc attctgacgc
    16201 cgatctcttc tttagatctc aatgcgctga gtaatcttcc cgcagccaaa gacgttgacg
    16261 ccgagcaatc cgcactggaa aacggcctga cgctggtact gaaaaacatt gagtttcgtc
    16321 tgctggatag cgacggtgct accagcgcga ttctggaagc gcaccgatcc ctggctggcg
    16381 atacttccct gcacgaacat ttactggcag gcgtcagcgc cggattaagc tgtgccgaag
    16441 cgattgtcgc cagtgcgaat cacttttgcg aagagtttgc ccgttccagc agcagatacc
    16501 tgcaagaacg tgccctggac gtacgcgacg tctgcttcca gttactccag caaatctacg
    16561 gtgagcaacg cttcccggca ccgggcaaac tgacgcagtc cgccatttgt atggctgatg
    16621 aactgacccc cagccagttc ctcgaactgg ataaaaatca cctcaaagga ttgttactga
    16681 aaagcggcgg caccacctca catacggtga tccttgcccg ttcgttcaac attccgacgc
    16741 tggttggtgt ggatattgat gccctcactc cgtggcagca tcaaacgatt tatatcgacg
    16801 gcaacgccgg ggcgattgtg gttgagccag gagaagccgt agctcgttat tatcagcaag
    16861 aagcccgcgt acaggacgcc ctgcgtgagc aacagcgtgt ctagctgacc caacaagccc
    16921 gtaccgctga cggtatccgc attgaaattg ccgctaacat cgctcactcc gtggaagcgc
    16981 aggccgcatt cggcaatggt gcggaaggcg ttggtttgtt ccgcactgaa atgctctata
    17041 tggatcgcac cagtgcaccg ggcgaaagcg agctgtacaa cattttttgt caggcgctgg
    17101 agtctgccaa cggacgcagc attattgtgc gcactatgga cattggcggc gacaaacccg
    17161 ttgattatct aaacattccc gcagaggcaa acccgttcct cggttatcgc gccgtgcgta
    17221 tttatgaaga atacgcgtcg ttatttacca cacaactacg ctcgatcctg cgcgcctccg
    17281 ctcacggcag cctgaaaatc atgatcccga tgatctcctc aatggaagag atcttatggg
    17341 tgaaagaaaa actggcagaa gccaaacagc aactacgtaa cgaacacatt ccgtttgatg
    17401 agaagatcca gctcggcatt atgctggaag taccgtcggt gatgttcatc atcgatcaat
    17461 gctgcgaaga gattgatttc tttagtattg gtagtaatga cctgacgcaa tatctgctgg
    17521 cagtggatcg cgataacgct aaggttactc gtcactacaa cagcctgaat ccggcattct
    17581 tgcgggcgct cgattacgac gtgcaggcgg tgcatcgcca gggtaaatgg attggtctgt
    17641 gcggtgagct gggagcgaaa ggttccgtgc tgccgttgct ggtcggctta gggctggatg
    17701 aactcagcat gagcgcacca tcaattccgg cggcgaaagc gcggatggcg caacttgata
    17761 gccgtgagtg ccgcaagttg ctcaaccagg caatggcctg ccgtacttcg ctggaagtgg
    17821 aacacctgct ggcgcaattc cgcatgaccc aacaggacgc accgctggtc accgccgagt
    17881 gcatcacgct ggaaagtgac tggcgcagca aagaagaagt gctcaaaggc atgaccgata
    17941 acctgctgct ggcgggccgc tgccgctatc cgcgtaaact ggaagccgac ttatgggcgc
    18001 gcgaggccgt tttctctacc ggtctgggct ttagttttgc cattccacac agcaaatcag
    18061 aacacattga gcaatccacc atcagcgtgg cgcgtctgca agcgccggtg cgctggggcg
    18121 atgatgaagc gcaattcatc attatgttaa ccctgaacaa acacgctgcg ggcgatcagc
    18181 acatgcgcat tttctcgcgc ctcgctcgcc gcatcatgca cgaagaattc cgtaacgcgc
    18241 tggttaacgc cgcctctgcc gacgctatcg ccagcctgct gcaacatgaa ctggaactgt
    18301 aaaaggaaac atcatggaac tgtatctgga caccgctaac gtcgcagaag tcgaacgtct
    18361 ggcacgcata ttcccgattg ccggggtgac aactaacccg agcattatcg ctgccagcaa
    18421 ggagtccatc tgggaagtgc tgccgcgtct gcaaaaagcg attggtgatg aaggcattct
    18481 gtttgctcag accatgagcc gcgacgcgca ggggatggtg gaagaagcga agcgcctgcg
    18541 cgacgctatt ccgggtattg tggtgaaaat cccggtgact tccgaaggtc tggcagcaat
    18601 taaaatactg aaaaaatagg gtattactac acttggcacc gctgtatata gcgccgcaga
    18661 agggttatta gccgcactgg caggggcaaa atacgttgct ccgtatgtta accgcgtaga
    18721 tgcccagggc ggggacggca ttcgtacggt tcaggagctg caaacgctgt tagaaatgca
    18781 cgcgccagaa agcatggtgc tgacagccag ctttaaaacg ccgcgtcagg cgctggactg
    18841 tttactggca ggatgtgaat ccatcaccct gcccttagat gtagcgcaac aaatgctcaa
    18901 cacccctgcg gtagagtcag ctatagagaa gttcgaacac gactggaatg ccgcatttgg
    18961 cactactcat ctctaaagga gcaattatgg accgcattat tcaatcaccg ggtaaataca
    19021 tccagggcgc tgatgtgatt aatcgtctgg gcgaatacct gaagccgctg gcagaacgct
    19081 ggttagtggt gggtgacaaa tttgttttag gttttgctca atccactgtc gagaaaagct
    19141 ttaaagatgc tggactggta gtagaaatta cgccgtttgg cggtgaatgt tcgcaaaatg
    19201 agatcgaccg tctgcgtggc atcgcggaga ctgcgcagtg tggcgcaatt ctcggtatcg
    19261 gtggcggaaa aaccctcgat actgccaaag cactggcaca tttcatgggt gttccggtag
    19321 cgatcgcacc gactatcgcc tcgaccgatg caccgtgcag cgcattgtct gttatctaca
    19381 ccgatgaggg tgagtttgac cgctatctgc tgttgccaaa taacccgaat atggtcattg
    19441 tcgacaccaa aatcgtcgcg ggcgcacctg cacgtctgtt agcagcgggt atcggcgatg
    19501 cgctggcaac ctggtttgaa gcgcgtgctt gctctcgtag cggcgcgacc accatggcgg
    19561 gcggcaagtg cacccaggcg gcgctggcac tggctgaact gtgctacaac accctgctgg
    19621 aagaaggcga aaaagcgatg cttgccgccg aacagcatgt agtaaccccg gcgctggagc
    19681 gcgtgattga agcgaacacc tatttgagcg gcgttggttt tgaaagcggc ggctggcggc
    19741 ggctcacgcg gtgcataacg gcctgaccgc tatcccggac gcgcatcact attatcacgg
    19801 tgaaaagtgg catttggtac gctgacgcag ctggtactgg aaaacgcgcc ggtggaggaa
    19861 atcgaaaccg tagctgcact tagccatgcg gtaggtttgc caataactct cgctcaactg
    19921 gatattaaag aagatgtccc ggcgaaaatg cgcattgttg caaaagcatc ttgtgcagaa
    19981 ggtgaaacca tccacaacat gcctggcggc gcgacgccag atcaggttta cgccgcactg
    20041 ctggtagctg accagtacgg tcagcgtttc ctgcaagagt gggaataacc tactccaaac
    20101 tcccggcttg tccgggagtt tgaacgcaaa attgcctgat gcgctacgct tatcaggcct
    20161 acgcaatctc tgcaatatat tgaatttgcg tgcttttgta ggccggataa ggcgttcact
    20221 ccgcatccgg cactctgtgc cagtaatctg aaactcccac gttgttcagg agttttttat
    20281 ccgcttctgg acattttctc cacagaacaa tcgttagccc ggaaggtcga atctgtgact
    20341 cttattttca cggacgaacc agatgaaagc ttcactggcg ctgctcagcc tgctaacggc
    20401 atttaccagc cactccttaa aatctcccgc cgttccacca accgtggtac aaatccaggc
    20461 caataccaac cttgccatcg cagacggtgc cagacagcaa attggcagca cgctatttta
    20521 cgatccggcg tatatgcagc ttacctatcc tggcggtgat gttccgcaag aacgcggtgt
    20581 atgttccgat gtggtgatcc gcgcattgcg cagccagaag gtcgatttgc agaaactggt
    20641 tcacgaagat atggcgaaga attttgctga gtacccgcaa aaatggaagt taaagcgccc
    20701 ggacagcaac atcgatcacc gtcgggtgcc taatctggaa acctggttta gccgccacaa
    20761 caaaacacgc cccatcagta aaaatgccgg tgactatcaa gcgggcgata ttgtctcctg
    20821 gcgattagat aacgggctgg cgcatattgg ggtggcgtca gatggcttcg cccgcgacgg
    20881 tacgccgctg gtgatacata acattggcgc aggcgcgcag gaagaagatg tgctgtttag
    20941 ctggcggatg gtggggcatt atcgttattt tgttaaataa gagatcgccc cacatgaaga
    21001 aaaaattcag atgcttttta cactgacagc ttcttttttc ttacggctca ccagcgccag
    21061 tgccagcaca atcagcacaa taccgctacc ttcaacggcc cccggattct caccgagcag
    21121 ccaccaggaa aagagtacgc cacaaacggg aacggccagt gtgcttaagc tggcaatact
    21181 ggcaggcaag tttttcagca caaacagcca taagctccac gctagcgccg tcgccagaat
    21241 cgcactgtag gccagcgccc agaacacggt gggctgccag tcaatttcac gttgcggcac
    21301 cagtaaagcg accacactca tcaccagcgc cgcatacagc atctgccagg atgttaacga
    21361 taataaatcc acgcgcggat gacgggcgta taggcgttta gcaacaatcg cgctcgcccc
    21421 ccaactaaca ccggagagga ttgccagcat ggcacttttc atcgaagaga aatcgagttg
    21481 ccacggctgc aacaccaaaa ataagccgaa agcggcaatc agaatcgcga aatattgccc
    21541 acgtcgcagg cgttcaccga gaaacaacgc ggcgaaaatc accacccaga acggcatggt
    21601 atagctcagg atcgccacct tccccgcacc tccgctgacc aacgcccact gcgccaaacc
    21661 aaccatcccg caggtttgta acagggcaat ggctaaggtg tatttaaacg gcgtcgggcg
    21721 cattccgcga ccacgtaata aaaggacgat gaataaaacg agagcgccga aaatgcagcg
    21781 taaggcggta aagtcgaagg caccgatgta actggtgact tgcttcatga aaatccagct
    21841 ataactccag ataagtgtga gcacaaccaa gccactgatt gccagtgggt tgctctttcc
    21901 tgcggcagac atgagagatc cagtgtgtag ttttcctgtg gacactatac gccggtaaag
    21961 aaacttcagc gactgcctga aagacagtcg ctgaagaagg tcagattaca gcaggtcgaa
    22021 acggtcgagg ttcatcactt tcacccatgc cgctacgaag tctttaacaa acttctcgtg
    22081 ggcatcgctg ctggcgtaaa cttccgctac tgcacgcagg acagagttag aaccaaatac
    22141 cagatccgca cggctggctg tgtatttcac ttcgccggtt tcgcggtcac gtccttcgaa
    22201 cagctctttc gattcgtcag tcgctttcca ctcgtaacgc atatccagca agttcacgaa
    22261 gaagtcattg ctcaatacgc caacgcggtc agtgaagacg ccgtttttgc tgccatcgaa
    22321 gttggcaccc agtacacgca tcccgcccac cagcgcagtc atttccggcg cggtcagcgt
    22381 cagttgctgt gctttgtcga tcagcagtga ctcggtggtg gaaacgtcca gacgagcgcg
    22441 atagttacgg aaaccgtcag caattggctc cagcagctca aacatctcaa tgtcagtctg
    22501 atcctgacgc gcatcaacgc gacccggcgc aaacggtaca tgaatgctca aacctgcggc
    22561 gcttgcggct ttctcaacac caaccacacc agccagcacg atgatatccg ccagcgaggc
    22621 tttaccagac tctttctgga ttttctccag aacaggcaga gcacgaacgg ctgcggcgtt
    22681 cacatcccag tcgcgctgcg gcattaatgc cagacgcgca ccgttggcac caccgcgttt
    22741 gtcgccacca cggaaggtag aagcagatgc ccaggccacc gataccagct cactaacaga
    22801 cagaccagaa tccgcaatcg cgaatttcag atcgataatg tcctgctcgg tcgggttgta
    22861 gatcggctgc ggcagcggat cttgccagat cagatcttct ttcggcactt ccggcccgat
    22921 gtagcgagat ttcggcccca tatccctgtg cgtcagtttg aaccaggcac gggcaaaggc
    22981 ttcgttgaac gcctgcggat cgttgaggaa acgacgagag atcttctcga actcaggatc
    23041 aaaacgcagc gtcaggtcgg tcaccagcat tgtcggttta cgtttcttcg acggatcgaa
    23101 cggatccggg ataatttccg gtgcgtctac cgcttcgaac tggattgctc cagccggact
    23161 gcgggtctgt acccactcat acttgaacag attctcgaag aaatagttgc tccactgggt
    23221 cggcgtctgg gtccagacta cttccagacc agaggtaatg gcatctgcgc caacgccgct
    23281 gccgtaagtg ctcgcccagc ctaaaccttg ttcttcaatc ggtgcagctt ctggatcagg
    23341 acctacgttt gatgtcggac cggcaccgtg ggttttaccc agcgtatgac cacccgcaat
    23401 cagcgccacg gtttcttcgt cgttcatgcc catgttgccg aaggtcgcgc ggatagctgc
    23461 tgccgcagaa agcggttcgc cgctgtgatc cgggccttcc gggttaacgt aaatcaggcc
    23521 catctcggtt gcacccagcg gtgctttcgc cagcgcttcc ggatgacggt gagtcagcca
    23581 ggctttttca tcaccccagt taacatccag atccggttcc cagacgtctt cacgaccggc
    23641 accaaaaccg aaggtacgga agccggagtt ttccagcgcc acgttacctg cgaggataaa
    23701 caggtcggcc caggagattt tctgaccata tttctgtttg attggccaca acaggcgacg
    23761 cgctttatcg aggcttacgt tatccggcca ggagttcagc ggtgcaaaac gttgctgacc
    23821 acgacccgcg ccgccgcgtc catcgattga acggtaagta cctgcgccgt gccaagccat
    23881 acgaataaac agaccggcgt agctgcccca gtcggctggc caccacggct gagattctgt
    23941 cagcagggct ttcagatctt ttttcaggcc gtagtaatct aatttgctga attctttgcg
    24001 gtagtcaaag tcctcaccca gcgggttaga acgattagaa tgttggttta acaggtcaac
    24061 acggagttga tttggccacc agtcgcgagt ggttgtgccc gcccccgcgc tctggtcgtg
    24121 accgccctga tggaacgggc atttgccagt ggctgtggtg ttatggatat cgtctgacgt
    24181 gctcatcaat gtgctcccct ctacagtgtt accgttacga tacacagcgt tagagagaag
    24241 ttataattga attaatccac ggatgcgata gttgagatct tacatattgt tggttaaaga
    24301 gatgtagatc aaattgatcg taattaagct tttttgatta tgtccgaatt cggacaaatg
    24361 ctttataaaa aagggtttta gcttcatatc cttcaagtaa tgagggagaa aacaaggctg
    24421 acaaccttat tttcccgccc tcatttcgag gcagcatttt gtgctctgtt taaaatttgt
    24481 gatcactgtg tgattttcac aaaagccaca ctatttataa accaggtcga acacccagcg
    24541 tatggcaaat cgcgtaactc atttcagcgc ggttaagcgt atagaagtgg aaatctttca
    24601 ctccttcacg gcttaaaatc ttcaccatat ccatggcaat attcgcgccc accagtttgc
    24661 gggtttcggc atcgtcatcc agcccgtcaa acatttgcgc catccacgcc ggaatacgca
    24721 cgttggtcat gtcggcaaat ttcttcgcct gtttaaagtt agataccggc aaaatgcccg
    24781 ggatgatttc cacatcaatg cccgccgata cacagcggtc acgaaaacgc aggtagcttt
    24841 cgacatcgaa gaagaactga gtaatcgcgc ggttggctcc ggcatccact ttgcgtttca
    24901 gattaagcaa atccgcctga gcgctttttg cttccgggtg aacttccgga tacgccgcca
    24961 cggatatatc gaaatctgcc acttctttta acagcgtcac caggtcagaa gcatacattt
    25021 ctggcttacc acttcccggc ggcagatcgc cacgcagtgc cacgatatga cgaataccgt
    25081 tattccagta gtcgcgtgca atggtgcgca gctcgtcggg cgtcgcatca atgcaagtaa
    25141 gatgcggtgc cgcttccaga ccagtgcgat ctttaatgcc tttaataatg ctgtgcgtac
    25201 ggtcgcgctc gccggagttc gcgccatagg tcaccgatac aaacttcggt ttcaggctgc
    25261 taaggcgatc gatggagttc cacagggtct gctccatttc actggtacgc ggcgggaaaa
    25321 actcgaacga aacattaatc tgcccctgga cttctgccag gctctgattc agggcatccc
    25381 gctggctggc gtgaaaaaag ctcatacctt acctcatcaa tcgcttgtag ttatatgttg
    25441 tgttgtgcac atctatccat ttagacgtcc agatgtaaag atgaaggaaa agccgaaggg
    25501 cgtcaaccga aaaatgacca atgatggtga ggaaaaatca gggaagatga aaaaacttca
    25561 ggatggtaag aaaaagaaaa tccgatatgc aaagcaaacc ggactgcaaa tataaactaa
    25621 ccatgtactt attgcttctt gccggattcg tcgtaaacgc ttcatccggc ctaattctat
    25681 gataattcaa tatatttcat tagaaaccat agacccgaaa acatgagtac cgggcattat
    25741 taaatttctg aaattacaac aactgtgcca gccggttgat atccgactga atcgccccgg
    25801 cggtgacgtc gcgcccagcg ccaggcccac ggatcaccag cggattatcg cggtaccagc
    25861 ggctttcgat ggcaaagacg ttatcgcatg gcagcagtga tgccagcgga tagtcttcac
    25921 gcaccgcctc cacgcccaca cgcgctttgc cgttggcatc gaaacgcgcc acgtagcgca
    25981 gcaccagccc catttcgcgg gccgcttcca gccgttgcac catctgctcg ttaagttcat
    26041 cgccattttc aaagaaatgg tcgatgctgc cgccttcgca atgagcaggc accagcgatt
    26101 ccacacgtac ctgatccggt tcaatgttgt aacccgcttc acgcgccaga atcaccagct
    26161 tgcgcattac gtctttgccg gagaggtcgt cacgcggatc aggctcggtt aagccctgct
    26221 gccacgcctg atccaccagc tcggtaaacg gcacgctacc gtcgaattgc aggaacagcc
    26281 aggagagcgt gccggagaag atcccactga tcgacaaaat ggtatcgccg ctgtcgatca
    26341 gatcgcgcac ggtatggttg atcggcaagc ccgcaccaac ggtggcgttg tatagccagt
    26401 gacgcccggt tttttcgaag gcgtcgtgga tctggcgata tttattgctg tcgcttgctc
    26461 ccgccagttt gttggcactg atgacgtgga aaccgtggct ggcgaaatcg agatactgat
    26521 cagcaagctg ctggctggcg gtaacgtcca acaccactaa atcatcatac ggatgggcgc
    26581 gcatccacag gaaaagcgac tcttcatcct gctcgaccgc ttcatcgttg aagaaggcta
    26641 acgcgcggct ggcgtccagc ccgtcataac tcaacagact gcggcggctg tccaccacgc
    26701 ctgccagcac aaattcaaag ccggtacgcg ccgaaagcgt gctctgctcg cgggcgaaca
    26761 gttccagcca gcgagagccg atattgcctt tgccgaacaa taccaggccg atgcgttttt
    26821 ctgcgcggaa gacggactga tgcagcccct gaatcaggct ttcggtcggg ccggtgcgca
    26881 gtactgccac cagactgatg ccgtcatcgg actgccacgt aaactcgacc ggctggcctt
    26941 tcagttgctg ccagaagcgg tggcaatgca gcgggttacg ggtgacaccc gccccgacca
    27001 tcgccaccag cgccagcccc tgacgcaggc gcagttctcc aggtaatccc gcttcgtcga
    27061 ggattttcag cgcactgtcg gccacttctg aggtgtagca aaattgcaac aactggcgat
    27121 cgttatgcac gccaaccgcc agtggacgca cctgggcacg tttcaggatc tggtcgatct
    27181 ctttatgcgc cagtttgaaa tcctgactgg cgggcaactg aaactcaatc aaacagacat
    27241 catcgtggct ggtgacaata cgcgcaccag taccggaggc cagcacgcgt tcaatgcgcg
    27301 tggaaccttg atccggcgtg tagctacagc gcagctgcag gtcgatttcg ctgccagaaa
    27361 ctggctgtaa agtacgggcg tgaagaacgg gagccgccag acgcgccagt tcgctggcct
    27421 catccagacg cagcaacggc agcaggcagg catctttcac tttacgcggg tcggcactgt
    27481 ataccccggc gacgtcgctc cagatggtta cacgagaaac acccgccagc gcaccgattt
    27541 gtgtcgcgga atagtcggaa ccgttacgcc ccagcaacac cgtttcaccg gcgttgttac
    27601 ggctgataaa cccggtcacc accagacgtt tgctcggatg ttgtaccagt agctgttgca
    27661 gcaacgggta agaaagccct tcatcaacct gcggttgcgc agcgcgttca gcgcgtaaaa
    27721 actcacgggc atcaagccag gccgctggca gcccttgttg attaagtacc gcagacatca
    27781 ggcgcgccga ccacacttca ccgtggccca ccacttccgc atacaccgca tcgttaatac
    27841 cactgtcgag caacgccgcc aggcgctcaa gatcgctgac aaaagcgcta atgaggctat
    27901 cggcttcttc agcgggtagc agaccgctaa tcagatcgca ctgatagcga cgcagcgttt
    27961 gttgaacctg atgcgcagag agacgatcgg tctggcttag tttcaaccag ttaatcaact
    28021 ggttagtggt gctaccggcg gcggaaacca ccatcatatc gtcaggctga gagaactccg
    28081 ccataatgcc cgcgacacgc aaataacact tcacatcagc cagactactg ccaccaaatt
    28141 tatgcagctg acgacctttc gcccctgcct gcgcaatcac actcattttt accccttgtt
    28201 tgcagcccgg aagccatttt ccaggtcggc aattaaatct tcgccatctt caataccggt
    28261 ggagatacgc agcagcgtct cggagatccc ggcggcagca cgcgcttctg gtgccatgcc
    28321 tgcatgtgtc atggttgcgg cgtgagagat taaactttcc actcccccta atgattccgc
    28381 cagcgtaaac aacgacagcc cgcccaggaa acgacacagc gtctgctcat cgccatccag
    28441 ttcaaaactc aacattgcgc caaagccttt ttgctgacgc gcggcaattt catgcccctg
    28501 attttccggc agcgacgggt gatacagttt tttcaccaac ggctgggttt gcagatattt
    28561 cacaatcgcc tgcgcgttgc gctgcgccag ctccatacgc ggcaccagcg ttcgcaaccc
    28621 gcgtagcagc agatagctgt caaacgcgcc gcccgtcacg ccaatattgt ttgcccacca
    28681 ggcaagttca gtgacaacgt ccgggtcttt agcaatcacc acgccagcca ccacgtcaga
    28741 gtgaccgttc agatatttcg tacatgaatg caacaccaga tcggcaccca atgccagcgg
    28801 attttgtaat gccgggctta agaaggtgtt atccaccacg ctcaccgccc cgacttccct
    28861 tgccagatgg cagattttcg caatatccac gacgcgtaac aatggattac ttgggctttc
    28921 taccagtacc agtttgggtt tttctgccag cgctgcccgt aatgcctgtt catcgccttg
    28981 atcaacaaac aacacgcgat agcaaccgcg tttcgccaga ctgtcgaaca ggcgatagct
    29041 accgccgtag cagtcgtgcg gcgcaaccag cagatcgcca ggtttcaaaa agacggtcgt
    29101 taccaggtga atcgcggaca tgccggtatt agtaagtact gcgccagcac cgccttccag
    29161 ttctgccagc gcacgctgaa ccacatcgcg cgttgggttg ccgcgacgcg agtaatcatg
    29221 cgcgcgcggt tcattaaatc cggtaaagtt ataggtgctg gaaagatgga tgggagggac
    29281 aacgcaacca tactgttcgt cgtcatttaa cccgctacgc actgcgatgg tggcctgttt
    29341 acgcgtcatg tgatgaagtt tcctgggctt tgtcggtgaa atgtcaggca ccagagtaaa
    29401 cattgtgtta atggacgtca atacatctgg acatctaaac ttctttgcgt atagattgag
    29461 caaatcccaa atagccgtta aaattatatg cattatcacg ccgacaggtg cattacacga
    29521 tgtcacggta acgcctgtac ggtaaactat gcgggtttac ggtcagtacc cacataaatt
    29581 gtggggtctg gtctcaattt attgacgaag aggattaagt atctcatggc tgaatggagc
    29641 ggcgaatata tcagcccata cgctgagcac ggcaagaaga gtgaacaagt caaaaagatt
    29701 acggtttcca ttcctcttaa ggtgttaaaa atcctcaccg atgaacgcac gcgtcgtcag
    29761 gtgaacaacc tgcgtcacgc taccaacagc gagctgctgt gcgaagcgtt tctgcatgcc
    29821 tttaccgggc aacctttgcc ggatgatgcc gatctgcgta aagagcgcag cgacgaaatc
    29881 ccggaagcgg caaaagagat catgcgtgag atggggatta acccggagac gtgggaatac
    29941 taaagcggaa aagagcggcg cggagtggaa tcgcctgatg cactacgctt atcaggccta
    30001 cgtcatattg caatttattg aatttgcacg aacttgtagg ccggataagg cgttcacgcc
    30061 gcatccggca taaacaacga gcacgttgtc tgcgacccac cgctttttat acatggacgt
    30121 ttaactatga aaaacaggct gctgatcctc agcctgctgg tttctgtacc tgcctttgcc
    30181 tggcagccgc aaactggcga catcatcttt caaatctctc gctcatcgca aagtaaagcg
    30241 atccaactgg caacccattc cgattacagc cacaccggta tgctggtgat gcgtaacaaa
    30301 aagccctacg tttttgaagc ggtcggcccg gtgaaataca ccccgctcaa gcagtggatt
    30361 gcccatggtg aaaagggcaa ctacgttgtt cgccgcgttg aaggcggact gagtgttgaa
    30421 caacagcaaa aactgacgca aacggcaaaa cgttatctcg gtaaaccgta tgatttcagc
    30481 ttctcatgga gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt ttaccagaac
    30541 gcgctgggaa tgcgcgtggg cgaacagcag aagttgaaag agtttgatct cagcaacccg
    30601 ctggtccagg cgaagctcaa agaacgctac ggtaaaaata ttccgcttga ggaaacggta
    30661 gtttcaccac aagccgtttt cgacgcgcca caactcacca cggttgccaa agagtggccg
    30721 ctgttttcgt ggtgatacga ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt
    30781 ttcggaaatc cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac
    30841 acggccaccg gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca
    30901 cacgtcgagg ttcaggtcat gaccaacggt ggagcggatt tttattacgt taccgcaaga
    30961 gcagctagca gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg
    31021 gtttaaggcc gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat
    31081 agcttcaata cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat
    31141 gcaaactgat ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca
    31201 ggatgatgct atgcccgttg cccacgttgc cttgcccgtt ccgcttcctc gtacctttga
    31261 ctatctgctg ccagaaggca tgacggttaa agctgggtgt cgcatgcgcg tgccgtttgg
    31321 caaacagcag gagcgcatcg ggattgtggt atcagttagc gatgccagcg aactgccgct
    31381 cactgagcta aaagcggtag tcgaagtgct ggatggtgag ccggtgttta ctcactccgt
    31441 ctggcgattg ctgctatggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt
    31501 tcatgccttg ccgattttac tacgccaggg gcggcctgcg gcgaacgcgc cgatgtggta
    31561 ctggtttgcc actgaacaag gtcaggcggt ggatctgaac agcctgaaac gctcacccaa
    31621 gcaacaacag gcgctggcag cgttacggca aggcaaaatc tggcgcgacc aggttgccac
    31681 gctcgaattt aatgatgccg cgttgcaggc gctgcgcaaa aaaggtctgt gtgatttagc
    31741 aagtgaaaca ccagagttta gcgactggcg aacgaactat gccgtttctg gtgagcggtt
    31801 gcgattgaat accgaacagg ccaccgccgt tggcgcaatt catagcgcgg cagatacttt
    31861 ttctgcctgg ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagtgt
    31921 actggaaaac gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct
    31981 gacaccgcaa actatcgccc gttttcgtga acggtttaat gcgcccgtgg aagttctgca
    32041 ttccggtctg aacgacagcg agcgtctttc ggcgtggctg aaagcgaaaa atggcgaggc
    32101 ggcgattgtg atcggcaccc gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat
    32161 tgtcattgac gaagagcacg acagttccta caagcagcag gaaggctggc gctatcatgc
    32221 ccgcgacctg gcggtgtatc gtgcgcatag cgagcaaatc ccgattattc ttggctccgc
    32281 aacgcccgcg ctggaaacgt tgtgcaacgt ccagcagaaa aaataccgcc tgctgcgcct
    32341 gacgcgccgg gcggggaatg cgcgtccggc aattcaacac gtgctggatt taaaaggtca
    32401 gaaggtgcag gcaggtctgg ctccggcgtt aatcacccgt atgcgccagc atttacaggc
    32461 caacaatcag gttatcctct ttcttaaccg ccgtggcttt gcgcctgcgc tactgtgcca
    32521 cgactgcggc tggattgccg aatgcccacg ttgcgatcac tactacacgc tgcatcaggc
    32581 gcaacagcat ttgcgctgtc accactgtga cagtcagcgt ccagtgccgc gccagtgccc
    32641 ttcctgcggt tccacgcacc tggtacccgt ggggctgggc accgaacagc ttgaacagac
    32701 gcttgcgccg ctgttccctg atgtgcccat ttctcgtatc gaccgcgata ccaccagccg
    32761 caaaggggcg ctggaacagc aactggcgga agtacatcgc ggcggtgcga ggattttgat
    32821 cggcacacaa atgctggcga aaggtcacca ctttccggat gtgacgctgg tggcgttact
    32881 ggatgtggac ggcgcgctgt tttctgccga ttttcgctcg gcagagcgtt tcgctcagct
    32941 ttacacccag gtcgctggtc gtgccgggcg tgcgggtaaa caaggcgaag tggtgctgca
    33001 aacgcaccat ccggaacatc ctctgttgca aacgttgctc tataaaggct acgacgcctt
    33061 tgccgaacag gcgctggctg agcgacggat gatgcaactg ccgccatgga ccagccatgt
    33121 gattgtgcgt gcggaagatc ataacaatca gcatgcgccg ctgttcctgc aacaactgcg
    33181 taatctgatc ctctccagcc cgctggcaga cgacaaactg tgggttctcg gtccggttcc
    33241 ggcactggca cctaaacgtg gcggtcgctg gcgctggcag atattgttgc agcacccttc
    33301 ccgcgtgcgc ttgcaacaca tcattagtgg tacgctggcg ctcatcaata caataccgga
    33361 ttcccgcaag gtgaaatggg tactggatgt tgatccgatt gagggttaaa ccgcttacga
    33421 tgcgaggcgg atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt
    33481 tttcacgcgc tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc
    33541 tgacgtgcca ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg
    33601 aaagacgttg ccctcaaggc aaaagtctct acagcgactg tctcccgagc attaatgaat
    33661 cccgataaag tctcccaggc cacccgtaat cgggttgaaa aagcggcccg ggaagtgggt
    33721 tatttaccgc agcctatggg gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg
    33781 attgtcccgg atatctgcga tcccttcttt agcgaaatta ttcgcggtat cgaagttacg
    33841 gcggcaaatc acggatatct ggtgctgatt ggcgactgtg cacatcaaaa tcagcaggaa
    33901 aaaaccttta tcgatttgat catcaccaag caaattgatg gcatgttgct gctgggttca
    33961 aggctgccgt ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg
    34021 aacgaatttg caccggaact ggagctgcct accgttcata tcgacaatct gaccgccgca
    34081 tttgatgcag taaattattt atatgagcaa gggcataaac ggattggctg tatagccggt
    34141 cccgaagaga tgccactgtg tcactaccgc ctgcaaggct atgttcaggc gctgcgtcgc
    34201 tgcggaatta tggttgatcc acaatacatc gcccgtggcg acttcacctt cgaagccgga
    34261 agcaaagcaa tgcaacagct gcttgatctt ccacaaccgc ctactgcagt cttctgccat
    34321 agcgatgtga tggcgctcgg cgcactttct caggcaaaac gccaggggct gaaagtcccg
    34381 gaagaccttt ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg
    34441 ctgacaacca tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt
    34501 gatcaaatgc aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc
    34561 atccggggat caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc
    34621 aaaggcccgc cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat
    34681 agtggcacaa cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag
    34741 cacctcacgg aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc
    34801 tgccgccgtt cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga
    34861 agagtccgag acgctgcaaa gccagaaagt gaccggaaac ggactaccac caaaaccaga
    34921 agaacgctgg cgctacatta aagagctgga aagtcgccag ccgggtgtgc gtgcgcccac
    34981 agaaccttct gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgtca
    35041 gcttcttgaa caaatgcagg ctgatatgcg ccagcagcca acgcagctgg ttgaggttcc
    35101 gtggaatgaa cagacgccgg aacagcgtca gcaaacgcta caacgccagc gtcaggcgca
    35161 gcaattagcg gagcagcaac gcctggctca gcagtcgcgt acgactgaac aaagctggca
    35221 gcagcagacg cgtacgtcgc aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc
    35281 agcttctact cagcagccgt accaggatct gctgcaaacc cctgcgcaca cgaccgcgca
    35341 gtcgaagccg cagcaagctg cgccggttgc tcgtgccgct gacgcgccaa aaccgacggc
    35401 ggagaaaaaa gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca
    35461 ggcagagacg gtacgtgctc agctggcgtt cgaaggattt gactcgaaaa tcaccaccaa
    35521 caatggctgg aatcgtgtgg tcattggccc ggtgaaaggc aaagagaacg cagacagcac
    35581 cctcaatcgg ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg
    35641 aaaccctcaa aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt
    35701 tgtactctgt attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt
    35761 aacggccatg tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa
    35821 ggcaacgtga aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc
    35881 ggtactgcgg atgcttttac gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag
    35941 ggccatctgg ttaaagccgc cgttgagctg gcaaaagact ggcgtaccga tcgcatgctg
    36001 cgcaaacttg aagcactgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt
    36061 aacggtgacg tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac
    36121 gcccaggctg cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct
    36181 gaaaaggcgt tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc
    36241 gaagaattaa gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc
    36301 gtcagcgaac tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt
    36361 gctctgcgta accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc
    36421 ccgaaaaata tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt
    36481 ctggctaagc tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt taccgaagtg
    36541 ggctacgtcg gtaaggaagt tgattctatt attcgcgatc tgaccgatgc cgccgtgaaa
    36601 atggtacgcg tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa
    36661 cgtattctcg acgtgctgat cccacctgct aaaaacaact ggggacagac tgaacagcag
    36721 caggaacctt ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat
    36781 gacaaagaaa tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct
    36841 ccgggcatgg aagagatgac cagccagctg cagtccatgt tccagaacct gggcggccag
    36901 aagcaaaaag cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa
    36961 gcggcgaaac tggtgaaccc ggaagagctg aagcaagacg ctatcgacgc tgttgagcag
    37021 cacgggatcg tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt
    37081 ccggatgttt ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcacc
    37141 gtttccacca aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg
    37201 ttccagattg cgaaaccgtc tgacctgatc ccggaactgc aaggtcggct gccaatccgc
    37261 gttgaactgc aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc
    37321 tctatcaccg tgcagtacaa agcactgatg gcgactgaag gcgtaaatat cgagtttacc
    37381 gactccggta ttaaacgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac
    37441 atcggtgctc gtcgtttaca cactgttctg gagcgtttaa tggaagagat ttcctacgac
    37501 gccagcgatt taagcggtca aaatatcact attgacgcag attatgtgag caaacatctg
    37561 gatgcgttgg tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt
    37621 ttcatcattg tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa
    37681 caacaaatta gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc
    37741 gcctttgctg caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatccg
    37801 ctggtcgccc tgctggcact aattaccgcc gggctattac agatcctttc taacctcgcc
    37861 aatgattacg gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc
    37921 ggcatgcaaa aaggggtcat tacccagcaa aagatgaaac gggcgctcat tattaccgtc
    37981 gtgctcatct gtctctccgg gctggcactg gttgcagtgg catgccatac gctggccgat
    38041 tttgtcggtt tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg
    38101 ggcaatcgtc cttatggtta tatcggtctg ggtgatattt ccgtactggt tttctttggc
    38161 tggttgagtg tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc
    38221 cttccggcga ccgcatgcgg cctgctggca acggcagtac tgaatattaa taacctgcgt
    38281 gatatcaata gcgaccgcga aaatggcaaa aacacgctgg tggtgcgctt aggtgaagtg
    38341 aacgcgcgtc gttatcatgc ctgcctgctg atgggctcgc tggtgtgtct ggcgctgttt
    38401 aatctctttt cgctgcatag cctgtggggc tggctgttcc tgctggcggc accattactg
    38461 gtgaagcaag cccgttatgt gatgcgggaa atggacccgg tggcgatgcg accaatgctg
    38521 gaacgtactg tcaagggagc gttactgact aacctgctgt ttgttttagg gatattccta
    38581 agccagtggg cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc
    38641 cacatagcgc gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg
    38701 atacttccga gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct
    38761 ccaactttgg cggacgggcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg
    38821 acaacgggtt gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg
    38881 atggcggtgg ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac
    38941 aaaatgaatg ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag
    39001 agttggatat cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgaaggca
    39061 ttggcgaaag cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc
    39121 tttatgccga caataccggg attattcttt cagaagatcc gctggatatt gaatgataag
    39181 aaaggcaccg caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt
    39241 acgcgaaaga tgaagcgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt
    39301 tcctgccagc cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc
    39361 tgatgctggg cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg
    39421 atttccatct gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc
    39481 tcaaacactt ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa
    39541 aaatcctcgt cccgattacc ggtgacgcct taataaatac gagcgcactt tagttagctc
    39601 cgattgtatg aagccgcgcc atcgctgtcc agcggcacgc cttgcagatt acggtttgcc
    39661 atacttttca tccttctcct agtgacataa tccacatcaa tcgaaaatgt taataaattt
    39721 gttgcgcgaa tgatctaaca aacatgcatc atgtacaatc agatggaata aatggcgcga
    39781 taacgctcat tttatgacga agcacacaca ttttaagttc gatatttctc gtttttgctc
    39841 gttaacgata agttaacagc atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat
    39901 acaacaaaca ttaactcttc aggatccgat tatgagtcaa acatcaacct tgaaaggcca
    39961 gtgcattgct gaattcctcg gtaccgggtt gttgattttt ttcggtgtgg gttgcgttgc
    40021 agcactaaaa gtcgctggtg cgtcttttgg tcagtgggaa atcagtgtca tttggggact
    40081 gggggtggca atggccatct acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc
    40141 tgttaccatt gcattgtggc tgtttgcctg tttcgacaag cgcaaagtta ttccttttat
    40201 cgtttcacaa gttgccggcg ctttctgcgc tgcggcttta gtttacgggc tttactacaa
    40261 tttatttttc gacttcgagc agactcatca cattgttcgc ggcagcgttg aaagtgttga
    40321 tctggctggc actttctcta cttaccctaa tcctcatatc aattttgtgc aggctttcgc
    40381 agttgagatg gtgattaccg ctattctgat ggggctgatc ctggcgttaa cggacgatgg
    40441 caacggtgta ccacgcggcc ctttggctcc cttgctgatt ggtctactga ttgcggtcat
    40501 tggcgcatct atgggcccat tgacaggttt tgccatgaac ccagcgcgtg acttcggtcc
    40561 gaaagtcttt gcctagctgg cgggctgggg caatgtcgcc tttaccggcg gcagagacat
    40621 tccttacttc ctggtgccgc ttttcagccc tatcgttggc gcgattgtag gtgcatttgc
    40681 ctaccgcaaa ctgattggtc gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga
    40741 aaccacaact ccttcagaac aaaaagcttc gctgtaatat gactacggga caattaaaca
    40801 tgactgaaaa aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt
    40861 aatggatcac gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc
    40921 aaaaccaggt tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct
    40981 ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac
    41041 gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc
    41101 cattgtctgg cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt
    41161 agaagattat atccgcagca ataccggtct ggtgattgac ccgtactttt ctggcaccaa
    41221 agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag cgtgcacgtc gtggtgaatt
    41281 gctgtttggt acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt
    41341 gaccgattac accaacgcct ctcgtaccat gttgttcaac atccataccc tggactggga
    41401 cgacaaaatg ctggaagtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc
    41461 ttccgaagta tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc
    41521 cgggatcgcc ggtgaccagc aggccgcgct gtttggtcag ttgtgcgtga aagaagggat
    41581 ggcgaagaac acctatggca ctggctgctt tatgctgatg aacactggcg agaaagcggt
    41641 gaaatcagaa aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta
    41701 tgcgttggaa ggtgcggtgt ttatggcagg cgcatccatt cagtggctgc gcgatgagat
    41761 gaagttgatt aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa
    41821 tggtgtgtat gtggttccgg catttaccgg gctgggtgcg ccgtactggg acccgtatgc
    41881 gcgcggggcg attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac
    41941 gctggagtct attgcttatc agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg
    42001 tatccgtctg cacgccctgc gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca
    42061 gttccagtcc gatgttctcg gcacccgcgt tgagcgcccg gaagtgcgcg aagtcaccgc
    42121 attgggtgcg gcctatctcg caggcctggc ggttggcttc tggcagaacc tcgacgagct
    42181 gcaagagaaa gcggtgattg agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa
    42241 ttaccgttac gcaggctgga aaaaagcggt caaacgcgcg atggcgtggg aagaacacga
    42301 cgagtaatgt caggcacctg aatcgattgc ctgatgctga tcgcctgatg cgacgcttac
    42361 gcgtcttatc aggcctacga gccaatgcca ttttgtgtag gcgggataag gcgttcacgc
    42421 cgcatccggc atcacgcaga ctctgaaacc gtgcgggggc aaccccgcac acatcaataa
    42481 tcccaccctt cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga
    42541 gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca
    42601 aatggttagg acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta
    42661 ttatgctcaa ccaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg
    42721 aagcaccgat gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata
    42781 ttgctgttga tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg
    42841 cggtgctggc agtaggcgat aaaggctgct tcctcaatgc gccggatatg tatatggaga
    42901 agctgattgt cgggccggga gccaaaggca ccattgatct gaacctgccg ctggcggata
    42961 acctgcgcaa tgtagcagcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc
    43021 tggctaaacc acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat
    43081 ttgctattcc ggacggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag
    43141 ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc
    43201 gcgcattaga tggcgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca
    43261 acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg
    43321 aagccggtaa agtattgcgt ctgggcgata tggcgcgcag cgataacgtc atcttctctg
    43381 ccaccggtat taccaaaggc gatctgctgg aaggtattag ccgcaaaggc aatatcgcga
    43441 ctaccgaaac gctgctgatc cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc
    43501 actatctgga tcgcaaagac ccggaaatgc aggtgcacat tctctgagtg atttgatcga
    43561 ttgagccttc cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa
    43621 gtcaatcaaa acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc
    43681 agaactggac cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg
    43741 ccgggcaatt taccaagctt ggccttgaaa tcgacggcga acgcgtccag cgcgcctact
    43801 cctatgttaa ctcgcccgat aatcccgatc tggagtttta cctggtcacc gtccccgatg
    43861 gcaaattaag cccacgattg gcggcactga aaccgggcga tgaagtgcag gtggttagcg
    43921 aagcagctgg cttctttgtt ctggatgaag taccggactg cgaaacgcta tggatgctgg
    43981 caaccggtac agcgattggc ccttatttat cgattctgca actaggcaaa gatttagatc
    44041 gcttcaaaaa tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc
    44101 cactgatgca agaactggaa aaacgctacg aaggcaaact gcgcattcag acggtggtca
    44161 gtcgggaaac ggcagcgggg tcgctcaccg gacggatacc ggcattaatt gaaagtgggg
    44221 aactggaaag cgcgattggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg
    44281 gcaatccaca gatggtgcgc gatacacaac agttgctgaa agagacccgg cagatgacga
    44341 aacatttacg tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt
    44401 atcgataaac ggcacgatga gcaaatccgc actcatctta ttgatcatcc cgcgatatgc
    44461 cggcatcaaa cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc
    44521 tttttgcata atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt
    44581 cttcggccat tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa
    44641 caattgaaga atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc
    44701 atcatcaata tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagggcttt
    44761 attcaccagt aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc
    44821 catgtttaac tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa
    44881 ataatcatct cggcctggca tgagaaagtg aaggccgcat caagctcaac ttgcccctcc
    44941 agatccttaa tcttataggc cacgctggcc ccttcatctg cagaggatgc agcggcggca
    45001 agctctttca gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa
    45061 aacttactgg tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta
    45121 gtactgcatt tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg
    45181 attacgactt aagaataatg cgccgttaag ggaagaacaa acaagtaatg atcttatgga
    45241 ttaccatttt ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggtattgt
    45301 catttgcatc gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg
    45361 ttaattactt gatttattgt cggctttata cttcacatcc tgagtatctt taccatattt
    45421 attttcgcct tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa
    45481 cgtcgggaca aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg ctcagttacc
    45541 cgccagcagc atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc
    45601 atgcaagcgc ttaacagtta ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc
    45661 cgcggtctga atatcgagta gattcttacc cgccagtgag aaaagcacca gcatgcctgc
    45721 gaaccacagg cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa
    45781 taaccattgc tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc
    45841 ttttgacaag ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc
    45901 aatgaagcca gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac
    45961 ggcgcatgca caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc
    46021 tactttcgat ctctccatca gccagtttcg agaagacttt aacagccaga atcccagcct
    46081 gccactgaac gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc
    46141 tgcaagtaaa attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa
    46201 aatcaaaagc attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa
    46261 agcgaaagct caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa
    46321 aacacaaagc cagaaaaaac tgcagtcgct actaacggcg gggaaaaaca aacgttatta
    46381 caccgagaca gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac
    46441 cttcgctgtt gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg
    46501 acaaaaagca aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct
    46561 gcggggcggc catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct
    46621 ttagtgatgg gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct
    46681 aacctgcgta aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa
    46741 atgtacatcg atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa
    46801 aacgtggacc tgaacctgtc cggcgcattc accggtgaaa cttctgccgc tatgctgaaa
    46861 gacatcggcg ctcagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag
    46921 tctgacgaac tgattgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt
    46981 ctgtgcatcg gtgaaaccga agctgaaaac gaagcgggaa aaactgaaga agtttgcgca
    47041 cgtcagatcg acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc
    47101 gcttacgaac ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag
    47161 gctgttcaca aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa
    47221 gtgatcattc agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag
    47281 ccagacatcg acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg
    47341 atcgttaaag ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata
    47401 tccggcactt actttcctta actcctcgcc ttaacgcaaa atctcacact catgatcctg
    47461 gatttcctcg gctgaagcac ggttcagcgt cagtagattt cgttgtgtcg ccagcaacac
    47521 aaacgaatta tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc
    47581 ctcaggcact tcttctgcaa gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt
    47641 tacccgacgc gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct
    47701 gctgatgttc gccagatttt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat
    47761 atgaatatga aaatggtttt gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc
    47821 gcgatcggga accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa
    47881 ccaaaagaag ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg
    47941 atacgttggc atcaacagat attgcagtgg accattaaga tcttttaaaa ccacgtatcc
    48001 ggcattgggt ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa
    48061 acattcctca aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta
    48121 accaataccg gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt
    48181 tttcatcttt ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa
    48241 taaaaaaccc ggtcgcgaga aaccaccggg tttgataatt atcctaggga aatcagcgtt
    48301 tgctgatctg atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc
    48361 cgccgaactc ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt
    48421 tcgctacttc agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg
    48481 gcgagtagag atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca
    48541 ctttatcgac caccgacacc gttggctctg cgaggataga ctcactcggc gtgacgattt
    48601 cgaatttatc tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca
    48661 gtacatcgcc aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca
    48721 gaacttcgac gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt
    48781 tgttgtgatg cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc
    48841 ttttcggatt aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga
    48901 tctgcttcgg attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt
    48961 tatccggcag acgtttgatc cactctttat caatccgccc gcgttccgca atcgcgtcca
    49021 cgtcataggc cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg
    49081 cttgtttacc tgaaccaccg tgtgactgac gaatcaccac gttatcacca gtttgctgtt
    49141 tccagtggga gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg
    49201 aaacgttaag aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta
    49261 accctacgcc ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc
    49321 gtgccagaag gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta
    49381 tatgttgagg gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt
    49441 tgtccatgcc ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat
    49501 aaattgcaga attcatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc
    49561 atcggtttca ggctaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg
    49621 cagtccagcc agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg
    49681 tggtgaacca gctgttcgtt ctggatacct acacaacgac cgccgtaacc tgccagcagc
    49741 agatcgatag cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa
    49801 ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg
    49861 atgaaatgcg ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt
    49921 tttttacctt tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca
    49981 acttccggaa ctacaacgaa ttcacagcca ccggcaatgg ccgcagccag ggtcagatcg
    50041 ccacaataac ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca
    50101 cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag
    50161 tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt
    50221 tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg
    50281 ataccacgtt ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc
    50341 gggaaacgcg cagaaccgag gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa
    50401 cggtctagct gtaccatacg gtcttcatac agacccagat agccgtcata aatacccatt
    50461 acttccagac cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct
    50521 ggcgcatcac cgccgcttgt caacacacct attttcttaa tcatgactac ctctgaactt
    50581 tggaatgcaa aatgaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga
    50641 atgtgcaaat agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg
    50701 gtattttata cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac
    50761 cgtttaccct ccctgggtac gacggaacag gggtcctgat ggataatcac atccgatccc
    50821 ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa
    50881 ggcagagagt cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc
    50941 gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct
    51001 tgccgttctt catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg
    51061 cgtaacgcgc tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga
    51121 tgccagccgt accaagacaa ccccagcgcc agcagaattg cgccgttcat cataacatca
    51181 gactggtaat gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccac
    51241 cgctgaaacg agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg
    51301 acgcctggat ctgtcatcgg tgttggagat accagatgtt gaatacccgt caaaaacagg
    51361 aatagtgccg aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctcagcttta
    51421 ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa
    51481 ttcgtcaacg acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc
    51541 gacccggtat accaccatgc aaaaatttta atcagcaata gcaacgaagc catcgccgtc
    51601 gcagcaatcg ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct
    51661 atcaactgac gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa
    51721 ttgaggataa aaaaaacccc acatcatgtg ggggaagaca gggatggtgt cacaaaaaag
    51781 cacctaacct gatgatataa atggatttat atcaaacgct gtccacatag cgtccacatc
    51841 gattaccgaa agccccaagc atatggggct ttattttttt ctttttttag atgtataaca
    51901 cttactcaac ccaatgttta ttaaggattt tctatggcaa ctcttaaatc aataatggca
    51961 ataaccacct taataatttc actcaatgca tacgcaaaca cgaatgacat taaaactctt
    52021 caaaaacaac tgaaaccatg gcaaccacta gaaatcacag caaaagatag ctctcttacc
    52081 gttgttctcc ctggtaaaaa aatcacatct gaagcctata atcatttaat tatgtccggt
    52141 gtatgcaccc cactttggac acatgacgct ccttcttcat ttttaaaaaa tgtaagtgaa
    52201 atcaatatag ttaaccaata taagtctatt ggatatacct ttgaagaccc attgaccaca
    52261 tgcaatgaaa tcgctccttt aatggaaaaa cctgcacaaa cagtggtgtt atccaaaact
    52321 catacctaca caggtaagta aatagctcaa ataagataaa gcccctcgac tgaggggctt
    52381 tctgtttgta attacatcca cataatttgc tgccctgacg gcaacggatg cggcctcacg
    52441 gcgtggactt ctcccggctt cacgatgtat cgctgtaccg actcataagt gatgaacgtg
    52501 gcgctgcaat tcacgttctg acactggtga taacgctctt ttgtcgtgtc agtgatatag
    52561 cggcttgtac gcgcatgtgc ggcatgctgg cataaaggac aatgaaacat cgcgagcacc
    52621 tcttccggtt ttgttgatgg tgccatttta gttaatttat ccttataaaa caaacagata
    52681 aacaatagac atcactcatc accttctgtt tcgtactcca catcagaaag cctgacctca
    52741 agctctaagg acgtcgtgaa gccgctatta ttcagaaaat gtgtcacctt agtgattgtc
    52801 cagtcctgct cgtctatgac gcgcttaaag cctgacactt taaccggtgt ttccgtgtaa
    52861 atatctgccc gaccggtagc caggctgatg gagaactccg caacgccccg ttgcagttta
    52921 tcccacttcg cctgagcggc gcgcatggcc tgcgctttcg tggcatatac cgtggtcagg
    52981 gcaaaaacgt tgtcagcctc accggccatg tattcacctt cacgcgcttc cggtacttta
    53041 ggcgctttct tctgcctgac cggtttcgct ttcgggtgct ccagtgcgcg caggtgtttc
    53101 tctttctttt tgcgtttcag ttttaccttc tgcttttgag gcttcgggtc tttggtgtgt
    53161 aaccactttg ccgttacgcc ggtgtaggct ccacggtcag caatcgcaaa atgatggcgg
    53221 tcgccgtcgc tgcgggttat ggtgacctgc gggatttttt taccgctgga cgtcaccccc
    53281 tgccccgctt tgagaaacag cagttttccc atttttaccg acacctcacc gccgttgcgt
    53341 tcagcaagac gggtcaggaa tttcgcatcg gactcctgcg actggtcgat gtgcgggatt
    53401 ttaattccgg ccagtgacgg agcgacactg gcttccagcc tgttacggga ggctatcgcc
    53461 tcaacaatcg caccgagcgt ggtgtcatgc caggagcctt cacggcggga attgagcgtc
    53521 ccgcgaaaat ctgcactccg ggcgcggatg gtaaccacat ccggcgcgcc ccggtgttca
    53581 acctcatcaa cggtaaattt ccctttgcat accagggcaa aacctttcca gccgatatac
    53641 accgtcagga cagcgccacg aaccggcagc ccgacctgcc cgtcggcatc gttcagttca
    53701 atatcaagct ggtcagcctc aaagccccgg ttatccgtca gggtcatgct catcagacgg
    53761 tcgctgatat tgccggtaat atccctgctg tcgagcatca gcatgtaatc cggcgtcagc
    53821 gtactgcctg catcaaatgt cagcgcatcc agcattatcc cgcccccgtc atacctgtga
    53881 atctggtcgc catactgccg gccttaccga tgagcgattc cgcctgttta ccgatatcgc
    53941 cataaagcgc ggccagtgat tcatcaacgc gggtgagtga cagcgtaaaa tcaattttcc
    54001 ggggtgtgcc gtctgcaaag aaaatactcc ctgtttcact caccctgctg atgacataca
    54061 tgccgtaaat catgccggtg ccatccagca acggccacgc ccgcccctcc tctgccatca
    54121 gcctgagcgt ggtcatcgtc agcttgccgc cggtcagttc gggataaagc acaccggcca
    54181 gcgtgatgtt ttcctcaccc acaccgagaa actgaaaagc gtcccgttta ccgatacggg
    54241 aatttgacgg ccagcgataa tctgattcac gctgcatggt ctggtgtggc agcgtctggc
    54301 gcataaaaac aaacatacct aacgcgagca tcatttttcg tcacctcctt aaccgtcatg
    54361 catcatgctg gcacgagcgc gcgcacgttt atcccgctcg tatttttcga gcgcatcctg
    54421 taactggcgg tcaagctgtg tccccggcgc agtgccaccc gtcaggctga tgtgatattc
    54481 gtttttactc tggtccacat aagagcggcc agccggtgcc gtgaccggct gataagcctg
    54541 atagcctgca taagagctgg tcgccggaat ataaccaccg ctgccatacg tggcggcttg
    54601 agttctggcg gcggtctggt caagtgtgtc tgactctttg ttgataacac cgagtttttc
    54661 cagtacccag tcaataccac tgcgcagttt gttgaacgca ttaagcggca gcatcagcgc
    54721 gtcagccagt gcctgcccga acatgacgcc cgtgtcacgg caacggttca gggtgtcctg
    54781 ggtggctttg accggggcaa tcaggttttt aaaccactgc cacgcggcct gtaacttttc
    54841 acccagccag tcaaacaccg gtttcagtgg cgtgaacagc tcccccaccg gcgcaaatgc
    54901 cgctttcagc ccttccacca caccgccaaa aaatgcgctg acaggctccc agtatttacg
    54961 gataagcaac gccccggcga caattgcggc caccacggcc acaaccggcc agctaatagc
    55021 cccgatggcg gtcataacag cactgccaac cgtcgtgaag actgccccca ttgcgcctgc
    55081 tgccgcgatg atggcattga tgccggtgat aaccggccag gcgacaagac caatggcacc
    55141 gatgatgcca ataagcgcca gtgcgccacc gacaatgagg ccgatggttg acgccagtga
    55201 tttgtttttc ttgatccagc cgtcgagttt taacacatac tttgtggccg tctgcgtgag
    55261 attacgcagc gcgccttcct gctggtcaaa caggtcagtc cccaccgcct cataagcgga
    55321 ctgaaactcc ttaaagtcac cgccgaggtt gtcctgcatg atatttacca gctcggcagt
    55381 cttcccgttt gaggctttaa acgcagcggt cagtttgtcc agctttccgg ttgaggcggc
    55441 ggtcatcagc acggcggcgg ctgagctggc ctcctccccg aaaatagttt tcatgtattc
    55501 agcctgctgg gcagtaccga gccggttttt ctcaaaactg gcctgcattt ctttcagaat
    55561 ggtaaatatt ggccgggtat ttcccttgct gtctgaggtt ttcacgccaa gctctttgag
    55621 tgcatcccat gcttttcccg ttggtgcctg caggcgactt aacacggcac ggcttcccgt
    55681 ccccgccatt gagcctgtaa tttttgcatc atgcagcgcc ccgaccattg cggcggtttc
    55741 ttcaatgctg acaccggcat tttttgccac aggtgcggca taggtcagcg catcgctcat
    55801 gccgtcaaaa tcggcggcgg ttttgttcat cgtcatggag agaacatccc cgatatgagc
    55861 gaccttatcg tttgaaagct gaaaggcgga tttcatcccc atcagcaggg cagcgttttc
    55921 ttccatcgtg cgacggttcg ccagcgccat attcagcgtg accggcgttg ccgcctgaat
    55981 ggcatcaaca tccccaccgg ctttcgcaat aataatctgc gcaccggctg catcatctgc
    56041 cgaggcggcg gtattgtcgc cgagctggcg cgcctgtttg cgtagtgcag ccatttcggc
    56101 ggagtctttt gccactccga gcacggcctg caattctgag tttttctgcg caaactcata
    56161 accgggcatc agcagcttaa ctccggccat cgttcccgca gcagcaatcc ccacaccggc
    56221 agcacccact gaggccatat ttccggccag ttccttgcct gcctgataac gctgtttgac
    56281 tgcgttaagt tttgcctgtt gtgcactgac acgcgccagc gcgtcacgct gtcggttaag
    56341 ctgtgcggtg gtttcactga tacggttttt cagtccctgc tcatcatgtg caagattgcg
    56401 ggtattaatt cccacagcgg ccagttcccg ctgctggcgt ttaacggaat ccgtcaggcg
    56461 gttatatttc gcctgtaagt cctccgccgc acgctttgcg gattccagca ctttcgcctg
    56521 agcacgggtc ggacgttcgg tgtttttaaa ctgtgtggca agggcttcgg cctcctgccg
    56581 agccttttca agtgcatgac cagtcacggc gagctgtgca ctggtcttgc gaaatccctc
    56641 aatacgggat gcctgaccgt tcagctcgcg cagtgatttt tgtgtttccc ggatatcccc
    56701 cgacagcgac ttgctcgctg tgcggatgga tttaaacggg cgggatgcct ggtcaacagc
    56761 cctgagcaat acctgtaatt ttacattgtt actcattcgt gtttccgctt cgccggagcg
    56821 ccttttcgcg ccatgtgatg agttcggtca ggctcatggg atacagttct gatggcggcc
    56881 agtgaaatat cactgccaca tccgccatca ggtcatcgac cgacagattt ttcggaaacg
    56941 tcactgcacc gagttcggcg acaaaaaacc gaccacctta ccggccagcg ccacaaggtc
    57001 aggcagttcc agcgcggcga cttcctgctc ggtcagcatc ggtgccgtca tgcgcggcag
    57061 cactttaatc agtgcatcga cttcggagtt tgcgaccgca gccagactga caccgcgcag
    57121 cgtccccgca ttgggtttca tcagcgtgac ctgttcgata acctgctcac cacgtttgac
    57181 cggattgtcc agggtaatga cattttcttt gttcatggtt ttctcacttc tgaatcaggg
    57241 ttaaccggtc agccaggctg accggatgaa aatcacaggc cgatattgcg gcggtgttgc
    57301 tccagccggt cgacgccgtt caccttctca atcatgttga tggtgtcgat ttcgaccagc
    57361 tccttaccgt ccatcgtcag ccggaaatag gtgcagacca cggagatttt cgactcggta
    57421 tcttctccct gtttcccctc gccggtgtcg atttctttct gacgtccacg catgaccacc
    57481 tcgacggcca ccgtttcgcc ggtatcgtcg cgctggtaag agccggcaaa acgaatcggt
    57541 acggcatcca caccggttgc ggcgtaaagc tcccagataa ccgaatccgg gaagccaccg
    57601 agcgaccact ccattgacag cgcatcgtca tcaaggccga ggtctaccgg tgcgctgccg
    57661 ttcattcccg caccgcgata gttttcgagc ttacgggtca gttttggtag cgtgacggac
    57721 ttcgcgacgc cctgatagct gtagccgttc agaaagacgt tcattaactt gagtttgcgc
    57781 ggcattgcca tcggtcaggc tccttaattg ctgttaaccg aggtgaccag atttgccagg
    57841 tatttatcgg taatacgctg gcgcagggtc aggttttcaa gaggaggcac cggggtatag
    57901 tcgtagtcga tatacagttt tccggccttg agggtttccg catcgttgga ttcttcgctg
    57961 aaccagcagg tcgcatccac gatatagccg tttgttttca gctcacggaa tttggcattg
    58021 atgccgtcaa cgatgtcgcg aatcagcgtt gcggtgatgg gcttgtccac cgcccacatg
    58081 tgcgcctcag ccatcgtgtc ggccagcacc tgcgcggtgc gggtgtagtt ttcaaagagg
    58141 aacagcgggt catcagagca ggtacggtta ccccagaatc ggaaaccgtc gcggcgaatc
    58201 agcgtagtga cgcctgactc gttaagcagg tcagcatcgg tgccggactc ctgcaaatcc
    58261 cagaatacag aggcgctgat gccggtaaca ccgtttaccc cgacgttgga cagcgtttta
    58321 tgccagccct gctcctggtc gattttagcg cgcagaccca gcgcacgggc ggtggcatac
    58381 gcggtggcgg tggtactggt gaccgtatcc catgcgagga aatccggcca gatgaccatc
    58441 agctcacgct ggctgaaatt ctggcggtag gctttcacct cggaaatggt tttacagccc
    58501 catgcgctga tatacccgaa agcgcgcagc ttctgacaga cggatgccag tgcaacagcc
    58561 acctctttgg tatccagtcc cggcacaccg agaatacgcg gtttaacacc ggttaccgac
    58621 tccgccgcca gcagggcttt cagtccggtg tactgaccgt tttcgtcagt ggtgccgatg
    58681 atattggaaa cggtctgcgc gagtttcgtt tcttcgtcgt cgccggttcc gtcttccacg
    58741 cgcacgacaa cggtgaccgg ttttgactgg tcagcgatgg cctgcaacga tgccgccagc
    58801 gtgccttttt taccggcctt tgcaatcgcg ctctgcacat tggtgatcag caccggttta
    58861 ttgacgggga aggtttccgc atccgcatcg ctggccgtgc agaccatgcc gacaatggca
    58921 gtggatacgg tggaaatgac gcgggtgccg tcgttaatct ccagcacctg cacgccgtga
    58981 tgatagtcac tcatccgttt aactccgtgg ttaatgggtg agtggtattt tcagttgtgc
    59041 cggagatgtc aggctatttg tcccggttgg ctaagggatg acacaattta ttctttgtcg
    59101 ctgatgaggg gaatttttta tagagcgtgg acaggccaat atcaaaaatc agcgccacgc
    59161 gttgacgaga ttcccccgca gccagcaacc gtccgacctg ctcccactca ctcgcggtga
    59221 gtttcggacg tctgccacca atacgacctt tggctctggc cgcttccagt ccggcgcgtg
    59281 tccgctcgac aatgagttct cgctccattt cagccagggc acccatcaca tgaaagaaaa
    59341 aacgccccat cggcgtgctg gtatcaatag catccgtcag gctgcgaaaa ttaatgccac
    59401 gttcgcgcag ttcctcaacc agaatgacca gatgccgcat actacgcccc agccggtcca
    59461 gcttccagac taccagtgta tccccctccg ataatgtcct gagcagtttt ttcagtcccg
    59521 gcctgtcgga cttcgtgccg ctgattttgt cctcaaaaat ccgctcacat cccgcgcatt
    59581 ccagcgcgtt acgctgcaat tccgtgttct ggtcatttgt tgatacgcgg acatagccaa
    59641 taagcatgat ggatcccctg aataaaaacc ggagatgatg ccagttagcc gttatctctg
    59701 cattttctta aacgttggtt tgggagaagg ctcagcgtta cccgttggtg tacctgttcc
    59761 gtggccttca gccactcctc caacaggctg gattaaatgc aacggtgcgc ctttttctgc
    59821 cgaagagtat ccggaactgg caaaagttta tccgacaaat gaattgccag atttacgtgg
    59881 tgagtttatt cgtggctggg atgatgggcg aggagttgat acggggcgcg gaattctgtc
    59941 tgctcagggg gatgctatta gaaatattac aggctggttt ggtgcacacg ccacagtggt
    60001 ggctaaacct ccgtttgtta aattttctgc gccggaaaca gcagaaaatg tcggggttgg
    60061 aggggggagt acgcgaaacg ctgtcaaact tgatttatcg ttatcggtcc ccacggcaag
    60121 cgaaaaccgc ccacgcagta tcgcatttaa ctatatcgtg agggctgcat aatgacacag
    60181 gcaaaattaa acagtgaatt tattgccaca gtagcgggtg atatgaccgt ctataactat
    60241 gacaacacga cacgggaata catttcatca tcaacagaat atcttgccgt tggcgtcggt
    60301 atcccggcat gttcttgttt agatgcacca gttacacata aagctggtta tgcaatctgc
    60361 cgttctgcag attttaactc atgggaatat gtgccagacc atcgcggtga aatcatctat
    60421 agcaccgaaa caggagaatc gaaagaaatc acagctctgg gtgattaccc tgaaaataca
    60481 accactatcg ccccgttaac gccatatgat aaatgggatg gtgagaaatg ggtgacggat
    60541 actgaggcac agcatagcgc cgcagtagaa gcggcagaag cacagcgcca gtcactgatt
    60601 gatgcagcaa tggcttccat cagtctgatt caactgaaat tacgggccgg acgtaaactg
    60661 acgcaggcag aaacaacccg ccttaacgct gtgctggatt acattgacgc ggtgacggca
    60721 acagatacca gcaccgcgcc ggatgtcatc tggcctgaac tgccggaggc gtaggccatt
    60781 caatatcgag tgctgttgaa gtattaacac gcatcaatag
//
LOCUS       DBKELA010000010        60404 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.010, whole genome shotgun sequence.
ACCESSION   DBKELA010000010 DBKELA010000000
VERSION     DBKELA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.010"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..306
                     /locus_tag="ACUQR0_000612"
     CDS             <1..306
                     /locus_tag="ACUQR0_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9107785.1"
                     /translation="NGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVY
                     KDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            419..1213
                     /gene="panB"
                     /locus_tag="ACUQR0_000613"
     CDS             419..1213
                     /gene="panB"
                     /locus_tag="ACUQR0_000613"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HHV9107786.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1225..2076
                     /gene="panC"
                     /locus_tag="ACUQR0_000614"
     CDS             1225..2076
                     /gene="panC"
                     /locus_tag="ACUQR0_000614"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HHV9107787.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2150..3052)
                     /gene="rpnC"
                     /locus_tag="ACUQR0_000615"
     CDS             complement(2150..3052)
                     /gene="rpnC"
                     /locus_tag="ACUQR0_000615"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HHV9107788.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3326..3706
                     /gene="panD"
                     /locus_tag="ACUQR0_000616"
     CDS             3326..3706
                     /gene="panD"
                     /locus_tag="ACUQR0_000616"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HHV9107789.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3710..4939)
                     /locus_tag="ACUQR0_000617"
     CDS             complement(3710..4939)
                     /locus_tag="ACUQR0_000617"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HHV9107790.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5003..5443)
                     /locus_tag="ACUQR0_000618"
     CDS             complement(5003..5443)
                     /locus_tag="ACUQR0_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHV9107791.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5548..6318)
                     /locus_tag="ACUQR0_000619"
     CDS             complement(5548..6318)
                     /locus_tag="ACUQR0_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9107792.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6315..7241)
                     /locus_tag="ACUQR0_000620"
     CDS             complement(6315..7241)
                     /locus_tag="ACUQR0_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9107793.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7350..8012
                     /gene="can"
                     /locus_tag="ACUQR0_000621"
     CDS             7350..8012
                     /gene="can"
                     /locus_tag="ACUQR0_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HHV9107794.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8053..8589)
                     /gene="hpt"
                     /locus_tag="ACUQR0_000622"
     CDS             complement(8053..8589)
                     /gene="hpt"
                     /locus_tag="ACUQR0_000622"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HHV9107795.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8795..11185
                     /gene="gcd"
                     /locus_tag="ACUQR0_000623"
     CDS             8795..11185
                     /gene="gcd"
                     /locus_tag="ACUQR0_000623"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HHV9107796.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11232..12782)
                     /gene="cueO"
                     /locus_tag="ACUQR0_000624"
     CDS             complement(11232..12782)
                     /gene="cueO"
                     /locus_tag="ACUQR0_000624"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HHV9107797.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12948..13295
                     /locus_tag="ACUQR0_000625"
     CDS             12948..13295
                     /locus_tag="ACUQR0_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HHV9107798.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13401..14267
                     /gene="speE"
                     /locus_tag="ACUQR0_000626"
     CDS             13401..14267
                     /gene="speE"
                     /locus_tag="ACUQR0_000626"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HHV9107799.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14283..15077
                     /gene="speD"
                     /locus_tag="ACUQR0_000627"
     CDS             14283..15077
                     /gene="speD"
                     /locus_tag="ACUQR0_000627"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HHV9107800.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15115..15477)
                     /gene="yacL"
                     /locus_tag="ACUQR0_000628"
     CDS             complement(15115..15477)
                     /gene="yacL"
                     /locus_tag="ACUQR0_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HHV9107801.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15652..18249)
                     /gene="acnB"
                     /locus_tag="ACUQR0_000629"
     CDS             complement(15652..18249)
                     /gene="acnB"
                     /locus_tag="ACUQR0_000629"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HHV9107802.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18604..20457
                     /locus_tag="ACUQR0_000630"
                     /pseudo
     CDS             18604..20457
                     /locus_tag="ACUQR0_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20528..21952)
                     /gene="lpdA"
                     /locus_tag="ACUQR0_000631"
     CDS             complement(20528..21952)
                     /gene="lpdA"
                     /locus_tag="ACUQR0_000631"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HHV9107803.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22277..24157)
                     /gene="aceF"
                     /locus_tag="ACUQR0_000632"
     CDS             complement(22277..24157)
                     /gene="aceF"
                     /locus_tag="ACUQR0_000632"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HHV9107804.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24172..26835)
                     /gene="aceE"
                     /locus_tag="ACUQR0_000633"
     CDS             complement(24172..26835)
                     /gene="aceE"
                     /locus_tag="ACUQR0_000633"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HHV9107805.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26996..27760)
                     /gene="pdhR"
                     /locus_tag="ACUQR0_000634"
     CDS             complement(26996..27760)
                     /gene="pdhR"
                     /locus_tag="ACUQR0_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HHV9107806.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28301..29674
                     /gene="aroP"
                     /locus_tag="ACUQR0_000635"
     CDS             28301..29674
                     /gene="aroP"
                     /locus_tag="ACUQR0_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HHV9107807.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29717..30571)
                     /gene="ampE"
                     /locus_tag="ACUQR0_000636"
     CDS             complement(29717..30571)
                     /gene="ampE"
                     /locus_tag="ACUQR0_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HHV9107808.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30568..31119)
                     /gene="ampD"
                     /locus_tag="ACUQR0_000637"
     CDS             complement(30568..31119)
                     /gene="ampD"
                     /locus_tag="ACUQR0_000637"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HHV9107809.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31207..32100
                     /gene="nadC"
                     /locus_tag="ACUQR0_000638"
     CDS             31207..32100
                     /gene="nadC"
                     /locus_tag="ACUQR0_000638"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HHV9107810.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32303..32743
                     /gene="ppdD"
                     /locus_tag="ACUQR0_000639"
     CDS             32303..32743
                     /gene="ppdD"
                     /locus_tag="ACUQR0_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HHV9107811.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32753..33900
                     /locus_tag="ACUQR0_000640"
                     /pseudo
     CDS             32753..33900
                     /locus_tag="ACUQR0_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33890..35092
                     /gene="hofC"
                     /locus_tag="ACUQR0_000641"
     CDS             33890..35092
                     /gene="hofC"
                     /locus_tag="ACUQR0_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HHV9107812.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35127..36170)
                     /gene="guaC"
                     /locus_tag="ACUQR0_000642"
     CDS             complement(35127..36170)
                     /gene="guaC"
                     /locus_tag="ACUQR0_000642"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HHV9107813.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36332..36370)
                     /locus_tag="ACUQR0_000643"
                     /pseudo
     CDS             complement(36332..36370)
                     /locus_tag="ACUQR0_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36395..37015
                     /gene="coaE"
                     /locus_tag="ACUQR0_000644"
     CDS             36395..37015
                     /gene="coaE"
                     /locus_tag="ACUQR0_000644"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HHV9107814.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37015..37758
                     /gene="zapD"
                     /locus_tag="ACUQR0_000645"
     CDS             37015..37758
                     /gene="zapD"
                     /locus_tag="ACUQR0_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HHV9107815.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37768..37965
                     /gene="yacG"
                     /locus_tag="ACUQR0_000646"
     CDS             37768..37965
                     /gene="yacG"
                     /locus_tag="ACUQR0_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HHV9107816.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38065..38454)
                     /gene="mutT"
                     /locus_tag="ACUQR0_000647"
     CDS             complement(38065..38454)
                     /gene="mutT"
                     /locus_tag="ACUQR0_000647"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HHV9107817.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38514..41219)
                     /gene="secA"
                     /locus_tag="ACUQR0_000648"
     CDS             complement(38514..41219)
                     /gene="secA"
                     /locus_tag="ACUQR0_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HHV9107818.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41281..41793)
                     /gene="secM"
                     /locus_tag="ACUQR0_000649"
     CDS             complement(41281..41793)
                     /gene="secM"
                     /locus_tag="ACUQR0_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HHV9107819.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42024..42941)
                     /gene="lpxC"
                     /locus_tag="ACUQR0_000650"
     CDS             complement(42024..42941)
                     /gene="lpxC"
                     /locus_tag="ACUQR0_000650"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HHV9107820.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43042..44193)
                     /gene="ftsZ"
                     /locus_tag="ACUQR0_000651"
     CDS             complement(43042..44193)
                     /gene="ftsZ"
                     /locus_tag="ACUQR0_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HHV9107821.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44254..45516)
                     /gene="ftsA"
                     /locus_tag="ACUQR0_000652"
     CDS             complement(44254..45516)
                     /gene="ftsA"
                     /locus_tag="ACUQR0_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HHV9107822.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45513..46343)
                     /gene="ftsQ"
                     /locus_tag="ACUQR0_000653"
     CDS             complement(45513..46343)
                     /gene="ftsQ"
                     /locus_tag="ACUQR0_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HHV9107823.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46345..47265)
                     /gene="ddlB"
                     /locus_tag="ACUQR0_000654"
     CDS             complement(46345..47265)
                     /gene="ddlB"
                     /locus_tag="ACUQR0_000654"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HHV9107824.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPIDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47258..48733)
                     /gene="murC"
                     /locus_tag="ACUQR0_000655"
     CDS             complement(47258..48733)
                     /gene="murC"
                     /locus_tag="ACUQR0_000655"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HHV9107825.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48787..49854)
                     /gene="murG"
                     /locus_tag="ACUQR0_000656"
     CDS             complement(48787..49854)
                     /gene="murG"
                     /locus_tag="ACUQR0_000656"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HHV9107826.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49851..51095)
                     /gene="ftsW"
                     /locus_tag="ACUQR0_000657"
     CDS             complement(49851..51095)
                     /gene="ftsW"
                     /locus_tag="ACUQR0_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HHV9107827.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51095..52411)
                     /gene="murD"
                     /locus_tag="ACUQR0_000658"
     CDS             complement(51095..52411)
                     /gene="murD"
                     /locus_tag="ACUQR0_000658"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HHV9107828.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52414..53496)
                     /gene="mraY"
                     /locus_tag="ACUQR0_000659"
     CDS             complement(52414..53496)
                     /gene="mraY"
                     /locus_tag="ACUQR0_000659"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HHV9107829.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53490..54848)
                     /gene="murF"
                     /locus_tag="ACUQR0_000660"
     CDS             complement(53490..54848)
                     /gene="murF"
                     /locus_tag="ACUQR0_000660"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HHV9107830.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54845..56332)
                     /gene="murE"
                     /locus_tag="ACUQR0_000661"
     CDS             complement(54845..56332)
                     /gene="murE"
                     /locus_tag="ACUQR0_000661"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HHV9107831.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56319..58085)
                     /gene="ftsI"
                     /locus_tag="ACUQR0_000662"
     CDS             complement(56319..58085)
                     /gene="ftsI"
                     /locus_tag="ACUQR0_000662"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HHV9107832.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58101..58466)
                     /gene="ftsL"
                     /locus_tag="ACUQR0_000663"
     CDS             complement(58101..58466)
                     /gene="ftsL"
                     /locus_tag="ACUQR0_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HHV9107833.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58463..59404)
                     /gene="rsmH"
                     /locus_tag="ACUQR0_000664"
     CDS             complement(58463..59404)
                     /gene="rsmH"
                     /locus_tag="ACUQR0_000664"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HHV9107834.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59406..59864)
                     /gene="mraZ"
                     /locus_tag="ACUQR0_000665"
     CDS             complement(59406..59864)
                     /gene="mraZ"
                     /locus_tag="ACUQR0_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HHV9107835.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14469 a        16731 c        14844 g        14360 t
ORIGIN      
        1 aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg agtcggtgta
       61 ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc taatgattca
      121 aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg aggagtttac
      181 ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct acaacaggat
      241 ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca ggtaacctac
      301 ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata aatcattgcc
      361 cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag gatacgttat
      421 gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc gtttcgcgac
      481 catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc ttaacgtcat
      541 gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc tgccagtaac
      601 cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa actgcctgct
      661 attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg aaaacgccgc
      721 aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt ggctggtcga
      781 aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag gtttaacccc
      841 acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg aagcgagcga
      901 tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc tggtgctgga
      961 atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc cggttattgg
     1021 tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg cctttggtat
     1081 taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg gcgacatccg
     1141 cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg gcgaagaaca
     1201 cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct gctgcgtcag
     1261 caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac tatgggtaat
     1321 ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga tgtggtcgtc
     1381 gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc acgttatcca
     1441 cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt agttttcgcc
     1501 ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt tgacgttcct
     1561 ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg cgtttcgact
     1621 attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg tgaaaaagat
     1681 tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga tattgagatt
     1741 gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg taacggttat
     1801 ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag ttcgattgct
     1861 gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc ggggcaagaa
     1921 ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga cacattgctg
     1981 gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct tggcgatgct
     2041 cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc tacggctgat
     2101 ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact taaataaggt
     2161 taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg tcttcccgag
     2221 acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt cttccctgct
     2281 gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagacttcc
     2341 ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc
     2401 gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa
     2461 tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca
     2521 gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga
     2581 tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc gagtaaaaca
     2641 tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg
     2701 gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg gcagcaatag
     2761 aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt tcaatcacaa
     2821 catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc gtgctgtgac
     2881 ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg agatcacaaa
     2941 gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcatgca
     3001 ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc atcttccctg
     3061 tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc gtcagccacc
     3121 tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg cgttacatgg
     3181 cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag tagacactca
     3241 tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc tgcttcgtta
     3301 acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg
     3361 cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt
     3421 tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg tcaccaacgg
     3481 caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa
     3541 cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca gcttcgttac
     3601 catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg aaggcgacaa
     3661 tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg
     3721 gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag atataaagtc
     3781 gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca
     3841 ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg ccaaacggat
     3901 acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat
     3961 caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa ccatctaccc
     4021 gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagctcag
     4081 aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt ttgatgcgtg
     4141 aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga tacgcatagc
     4201 ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga
     4261 tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat cccctgtcgc
     4321 gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa gtatggcgaa
     4381 aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc aggccattat
     4441 cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc
     4501 aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca
     4561 gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta tctttccagg
     4621 taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct tcaacttttt
     4681 gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca aagccaaaat
     4741 taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg gcccgaatat
     4801 tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt tcgatggttt
     4861 gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc agcaggagaa
     4921 taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt tattgtagca
     4981 aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg acgaaaggcg
     5041 gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt tcgacaatac
     5101 actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt tcaattaacg
     5161 gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat aatgaagcca
     5221 cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca ccagagtccg
     5281 cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat ccgcgccaga
     5341 aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca tccagtatct
     5401 cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc ctccttagcc
     5461 gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa actcctctcc
     5521 cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac gttggatcag
     5581 cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa aggtagtgac
     5641 cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca tataaacgat
     5701 tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg agtaaaagac
     5761 cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga tgtcatcgaa
     5821 cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg ccgtgagcac
     5881 caaggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa acagtgaaat
     5941 tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga catatccggc
     6001 aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac gctggaactt
     6061 ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg acatcatgat
     6121 cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa tacgcgaacc
     6181 tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg gcaccagcgt
     6241 ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt ttagcgccac
     6301 ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga aacaaacagc
     6361 tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg ctcacttaac
     6421 tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag cgtcgcggta
     6481 tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg tgcgagatcg
     6541 agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt attttccacc
     6601 agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc ttccaggtag
     6661 tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt
     6721 gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag tagtttaggt
     6781 tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga taacatacgc
     6841 gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc gctgcggatg
     6901 tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat ttgctgaacg
     6961 gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg tttagcgttc
     7021 acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt tttatttacc
     7081 agagagctga taataccgat agcggtcgat ttcccggccc cgttcggccc gagaagcgca
     7141 taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac gccgcctgga
     7201 tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta cttaccttac
     7261 gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt agccaaacgt
     7321 aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc atcagcaaca
     7381 atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag aaactggcac
     7441 aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt cctgcagaac
     7501 gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct aacctagtca
     7561 ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta ctcgaagttg
     7621 aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc gttgaaaacc
     7681 cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg ttcaaacata
     7741 gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt gaactgaacg
     7801 tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg tggaaacgcg
     7861 ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg ctgcgtgatc
     7921 tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca
     7981 acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc aacacatccg
     8041 gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca gatgacggta
     8101 acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg agaaaccgat
     8161 aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg tacaaatcgc
     8221 caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg tattccccga
     8281 gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat ccagatcttt
     8341 gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg cggtcataaa
     8401 gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca taaatgagcc
     8461 acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct cagtaatctg
     8521 acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta cttctacagt
     8581 atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg gcaggttttg
     8641 gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt cgcggaatct
     8701 gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa ttaattgcta
     8761 ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata cagcctcgcg
     8821 acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt atctactcat
     8881 tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg ctggccttgt
     8941 gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt ggctatacgc
     9001 agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg acttctgggc
     9061 gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc tgccgtttgt
     9121 ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg tcgcactgct
     9181 gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga tcaacggcac
     9241 cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc aggactggcc
     9301 tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa ttcacgccga
     9361 taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga agcagccgaa
     9421 cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata ccctttacct
     9481 gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag agaaatggca
     9541 ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc gtggcgtctc
     9601 ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt gcccgcgtcg
     9661 tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa acggcaaact
     9721 gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc cagacaccaa
     9781 accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca tcgtgatggc
     9841 cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga
     9901 tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc cgaacgcaat
     9961 cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac cagcggccta
    10021 tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg atatctgggg
    10081 cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc tgaatgccac
    10141 taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg acatggatct
    10201 tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc cgattattta
    10261 cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg aactggtggt
    10321 tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg ttacccctac
    10381 tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg cggatatgtg
    10441 gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga
    10501 aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta acctggggat
    10561 gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat
    10621 ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga tggagcagcc
    10681 gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg gtgtaccgta
    10741 tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac agccagcatg
    10801 gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga aacgtattgg
    10861 tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca atatgggtat
    10921 gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg ccgctacggc
    10981 agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc agggtcgttt
    11041 accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc agtacgtggt
    11101 gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata ttgtggctta
    11161 tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc atatttccga
    11221 atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg ctccagcaga
    11281 tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt caccagcact
    11341 tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg agccgctggc
    11401 ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa cggatgcagc
    11461 atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc cgccgcaaac
    11521 atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg gaaatcgaac
    11581 ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat catctggctg
    11641 tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg catccccatc
    11701 atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa
    11761 ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact aatagcaatc
    11821 ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat ccccatctgg
    11881 ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac cagcacttca
    11941 aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg tagcagacca
    12001 ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg
    12061 gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc gtgttgtggg
    12121 tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc ggtcatcaca
    12181 tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac gatcaccgga
    12241 acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc atcttcaatc
    12301 accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg ttgatgtgga
    12361 tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct
    12421 ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag cccgtgccag
    12481 tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc tttgccgcgc
    12541 tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc
    12601 tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc
    12661 gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc acggctccac
    12721 agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc acgacgttgc
    12781 atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc
    12841 cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg
    12901 ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt
    12961 tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac gcgctcagcg
    13021 agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt
    13081 gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc
    13141 aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc ggtgaagaca
    13201 gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa gccctggccc
    13261 gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata
    13321 ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt
    13381 aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc
    13441 agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccagg
    13501 atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac
    13561 aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg
    13621 cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
    13681 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg
    13741 tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac gatccgcgct
    13801 ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg
    13861 tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc acctcagcat
    13921 tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca caaaacggcg
    13981 tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca
    14041 gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg
    14101 catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt
    14161 ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg
    14221 ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga gaagataaga
    14281 aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta
    14341 tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg
    14401 atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg
    14461 gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc agcgtcacta
    14521 ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc
    14581 cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta catacctacc
    14641 cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa gtctctacct
    14701 gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata
    14761 tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt atgaagcact
    14821 ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt
    14881 atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag atgttgctta
    14941 aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg
    15001 agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca
    15061 atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat ttatttactt
    15121 ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag
    15181 cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc
    15241 cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt attcatgccc
    15301 tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc
    15361 aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc
    15421 catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt
    15481 gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg taattatcgc
    15541 agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa tgcccgggaa
    15601 tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc
    15661 agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt tgaagttcag
    15721 ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct ggtactcttc
    15781 cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag aagccaggaa
    15841 gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg aaaccaccgt
    15901 cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg ggatctcgat
    15961 acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt gcgcggcgtc
    16021 catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt gcgcatccag
    16081 cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga taaatacttc
    16141 gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat cgttcggagc
    16201 acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg cgtattccgc
    16261 atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg
    16321 gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat
    16381 gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac aaccagcggc
    16441 agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca gatccggcag
    16501 accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct tctcaacggt
    16561 cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc gcagggtgat
    16621 gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat
    16681 tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag agataccgat
    16741 cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca gcatacggtt
    16801 cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat
    16861 gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg
    16921 gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc
    16981 atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt tcggttcgca
    17041 gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tctgcgccag
    17101 cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg
    17161 cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca gcggaatacg
    17221 gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag
    17281 ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc
    17341 catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt
    17401 aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac
    17461 atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga
    17521 acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc
    17581 aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt
    17641 tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat ccggtgccgg
    17701 agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg tcagtttttc
    17761 agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca ttacctgctt
    17821 cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag
    17881 cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat ccagcgcgtc
    17941 gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga tagctttttc
    18001 cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga agcctgcttt
    18061 tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat ctaacaggaa
    18121 ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca tttggtttgc
    18181 atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc
    18241 ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac
    18301 gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca
    18361 ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg
    18421 ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata
    18481 atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact
    18541 cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga
    18601 attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg
    18661 ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc gcctgtagaa
    18721 ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc tgcaacggtt
    18781 tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc acaaatagat
    18841 caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt gctgatggca
    18901 tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa
    18961 caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca
    19021 ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac
    19081 ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg
    19141 caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt tattaccaca
    19201 acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc atccaatacc
    19261 gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc cattgagccc
    19321 gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg
    19381 gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttgttgac
    19441 agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc actattcagc
    19501 agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga tgattatcat
    19561 caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga caacatcaat
    19621 atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca
    19681 tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag
    19741 cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc tgctccaaca
    19801 cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc
    19861 gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaacac
    19921 tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac ccagcagcaa
    19981 aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca ggcagtccgc
    20041 gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg ccagcgtatt
    20101 cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac taacccgcag
    20161 aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc cgaacagcgc
    20221 caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg tgataacgcc
    20281 cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga gaaagttcag
    20341 gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa taacgagcaa
    20401 cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga aaggtaaatt
    20461 gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg ttccggcaaa
    20521 cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca
    20581 cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga
    20641 tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc agctcgccgc
    20701 cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct
    20761 tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc cacgggaagg
    20821 tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg
    20881 caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag tagtgtttct
    20941 taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg
    21001 gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt
    21061 caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt
    21121 taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagctg
    21181 gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa
    21241 ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga
    21301 tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac
    21361 ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc attaccagca
    21421 ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt
    21481 catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcattgtcga
    21541 agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc ccggtgaatt
    21601 tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg
    21661 tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt
    21721 cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt
    21781 gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt
    21841 aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac
    21901 ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct
    21961 ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt
    22021 cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc gcttgctacc
    22081 ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt
    22141 tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat
    22201 ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt
    22261 gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga
    22321 tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa
    22381 tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact
    22441 tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac
    22501 ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct
    22561 taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt
    22621 tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac
    22681 cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga
    22741 agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga
    22801 tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca
    22861 gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc
    22921 tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg atttcgccaa
    22981 acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg ccagtcgccg
    23041 ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca
    23101 ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca aactcgcgtg
    23161 ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca aattcagatt
    23221 tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc gctgccgctt
    23281 cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    23341 aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    23401 gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac tgttcagcgg
    23461 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    23521 ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc ggagtcgctt
    23581 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    23641 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtacctgcaa
    23701 acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc gactgttcag
    23761 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    23821 tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt
    23881 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    23941 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    24001 tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    24061 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    24121 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    24181 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    24241 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc
    24301 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    24361 acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    24421 catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga tatacggaac
    24481 gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac catcacgcgc
    24541 cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    24601 gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca gctgaacttt
    24661 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    24721 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
    24781 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
    24841 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
    24901 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    24961 gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc cgatacgctg
    25021 gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt tcgtgctgta
    25081 agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag
    25141 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    25201 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    25261 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    25321 cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct gctcttccag
    25381 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    25441 gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat gctcttcaga
    25501 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    25561 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    25621 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    25681 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    25741 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    25801 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    25861 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    25921 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    25981 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    26041 aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt cacgggtagc
    26101 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    26161 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    26221 ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact gccagaattc
    26281 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    26341 gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt aaacgcccgg
    26401 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    26461 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatgtggcc
    26521 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    26581 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    26641 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    26701 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    26761 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    26821 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    26881 gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta gttaccgcgc
    26941 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    27001 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cggcttctgt
    27061 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    27121 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    27181 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    27241 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    27301 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    27361 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    27421 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag
    27481 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
    27541 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
    27601 cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga
    27661 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
    27721 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
    27781 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
    27841 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
    27901 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaagg
    27961 gattttttaa cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt
    28021 tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga gaggggttga
    28081 ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg
    28141 gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc
    28201 aaacttcctt gatgtaaata aatcaataca acaaacggaa ttgcaaactt acacacgcat
    28261 cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca
    28321 cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg
    28381 cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg cagggccagg
    28441 gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg
    28501 tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg
    28561 gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc
    28621 catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac
    28681 ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa
    28741 agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg tagtagcgat
    28801 gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag
    28861 caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat
    28921 ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc
    28981 tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc gcatcctgat
    29041 tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc
    29101 cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc
    29161 gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa
    29221 cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc tggcgtctgt
    29281 cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg
    29341 cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt
    29401 atccgcactg gtgatcaact ggacgatgat cagtctggcg catatgaagt tccgtcgcgc
    29461 caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg
    29521 gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc
    29581 gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa
    29641 agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca cccgtacagg
    29701 gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca atcaccacca
    29761 cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat
    29821 cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc tgatattgcg
    29881 aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca ccatgaccga
    29941 tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcactg
    30001 catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc
    30061 atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca
    30121 gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct
    30181 cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc
    30241 cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat
    30301 aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca
    30361 gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacaccca
    30421 tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga
    30481 acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa
    30541 ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa
    30601 ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg
    30661 gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc
    30721 aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc
    30781 aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc
    30841 acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc
    30901 ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc
    30961 agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg
    31021 caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg gtgagggaac
    31081 gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg
    31141 tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt
    31201 tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct gctggaacgc
    31261 attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca
    31321 gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc
    31381 acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga agaggtgttt
    31441 attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc
    31501 aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa
    31561 cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat
    31621 gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc
    31681 ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg
    31741 ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc agtgcgccag
    31801 gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga agtagagaat
    31861 ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct ggataacttc
    31921 gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg
    31981 tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc
    32041 tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa
    32101 ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt
    32161 cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg
    32221 ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa agtagcacca
    32281 accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat
    32341 ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt atcaaaacta
    32401 cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc gtaccgccgt
    32461 agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat
    32521 tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc
    32581 gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag gttgggataa
    32641 cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca
    32701 gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat
    32761 tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg ccagcgaaga
    32821 agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg cattgcattt
    32881 cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggtcacgc
    32941 cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc ccaaagcaga
    33001 attgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata ttcatatcga
    33061 accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc
    33121 ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc tgggaaacct
    33181 ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa
    33241 caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag
    33301 gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc agccgttaca
    33361 actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc
    33421 tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa ttccaccact
    33481 gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt
    33541 acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg ccgacagcaa
    33601 ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca ctggcaggca
    33661 cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt tgaagtcctg
    33721 cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga atcgctggaa
    33781 acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag
    33841 caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcaagtaa
    33901 gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg
    33961 ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta ccccactaag
    34021 cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat
    34081 tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc tggctctgct
    34141 ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct
    34201 cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat ttccgccgct
    34261 ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga
    34321 actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat cagcgttgcg
    34381 ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt
    34441 tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca
    34501 ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg tgttgttcgg
    34561 ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc ttatcgcgcg
    34621 gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac aaaaactcac
    34681 gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt tacagggcgt
    34741 agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca
    34801 gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg gtgagtttag
    34861 cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt
    34921 agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc
    34981 cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc
    35041 aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg
    35101 tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt ttcttgttcc
    35161 agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc cccaacgtat
    35221 gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac ctggcctcgc
    35281 agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc gccaacgtga
    35341 cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc
    35401 tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa
    35461 tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc accgtcgctg
    35521 acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc agaaagctgc
    35581 ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact
    35641 ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg
    35701 ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag
    35761 tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg gttcaggtcg
    35821 agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac catcacatgt
    35881 ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac agaaaagtgt
    35941 ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga aaatgtgcct
    36001 acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc tgaatgtttg
    36061 aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg
    36121 cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc
    36181 ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa
    36241 tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc
    36301 tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt tttaacgttt
    36361 tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg ccttaacggg
    36421 aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt
    36481 cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc
    36541 cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc agcgccgggc
    36601 cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg acctgctgca
    36661 tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc cctatgtact
    36721 gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt
    36781 ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg
    36841 cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc
    36901 agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct
    36961 gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag
    37021 acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag
    37081 tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat
    37141 ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag
    37201 ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc
    37261 gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt gagcgtatta
    37321 atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt
    37381 cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg
    37441 ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac
    37501 ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt
    37561 aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc
    37621 ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt
    37681 gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc
    37741 gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc
    37801 tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt
    37861 tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc
    37921 gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc
    37981 tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga
    38041 tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta ccggttcatt
    38101 ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac cgggttgccc
    38161 ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca gagttatatg
    38221 cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc
    38281 gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca tttcaatttt
    38341 accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac gtgttataaa
    38401 gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt tcattgtctt
    38461 aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag cttttattgc
    38521 aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg atcgttacgt
    38581 cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc
    38641 tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg ctgttgttcc
    38701 agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact gataacttca
    38761 tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt gtattcctgc
    38821 ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata gtccatcgct
    38881 gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt ctcgaagtga
    38941 cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat ggactgcgcc
    39001 agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag ccactcggca
    39061 attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg aatatcccac
    39121 atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa cacatcttca
    39181 cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt acgctgggag
    39241 taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg cttacgaatg
    39301 tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt cacccacggg
    39361 tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga tactcggtcg
    39421 gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga agaaccagca
    39481 tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga ttcgtgacgc
    39541 tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg tacctgccag
    39601 tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc
    39661 tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt cgcgatagtc
    39721 accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg
    39781 ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag ctccgatttt
    39841 tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc tttgatatct
    39901 tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg cagatcttta
    39961 cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat ggagctaaat
    40021 tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata cagacggaag
    40081 tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg cacaccttct
    40141 ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat ggtacgaccg
    40201 gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac gtcacgggta
    40261 aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat gttggccgga
    40321 gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc ttcaatcagc
    40381 accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga gaagtggcct
    40441 tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg aataatttta
    40501 ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat cagcggtgta
    40561 cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg cagtttacgc
    40621 tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa gccgtattcg
    40681 ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg tgctggcatg
    40741 cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg gttgttttcg
    40801 gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt accggttagt
    40861 gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt acgcatttcg
    40921 gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa gtgacgcata
    40981 ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat cagattttcc
    41041 agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt cagttcttca
    41101 tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac tttgcgcatc
    41161 cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt gattagcata
    41221 ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg tttattgttg
    41281 ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc agacgggcgt
    41341 gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct tttcagacgg
    41401 cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg
    41461 aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga aagaaagatg
    41521 acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt tcgaattcgg
    41581 gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc
    41641 gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg
    41701 caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt agcgtttacc
    41761 aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat cgaattcaaa
    41821 cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga atgaagataa
    41881 gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag ctcttataaa
    41941 attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg ccagtcgaat
    42001 tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt gaaggccaac
    42061 ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt cgccaggaca
    42121 gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa tgcaccaata
    42181 atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg acgcacaaat
    42241 tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac aacgatggca
    42301 caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat atcacgcatg
    42361 aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga gaagttcatc
    42421 gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt gaaatccagc
    42481 gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac acgaacagtc
    42541 tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc gtcaagcagc
    42601 aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc gttaacttcg
    42661 ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc tacggttgaa
    42721 atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg cacagatttg
    42781 gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat gaccccggtg
    42841 ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa accgacaccc
    42901 gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt
    42961 acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa agagcctcga
    43021 aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg ggatatccag
    43081 ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag caaccggctt
    43141 ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca ctggctgctg
    43201 aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga tacctgtcgc
    43261 aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac cgataaccac
    43321 agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga actcatccag
    43381 acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag acaggtcgat
    43441 atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac ggtcttcacc
    43501 gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg tgcgtacgtc
    43561 tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac cttgcacagc
    43621 gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac cgcggcccag
    43681 aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct tggacagttc
    43741 agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga aaggcttagt
    43801 gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga ctggtgctgc
    43861 acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg caccttccag
    43921 cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt ctggattagc
    43981 gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc caaccgctgt
    44041 tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt caatacgctc
    44101 gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga ctttaatcac
    44161 cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg tgcctgtcgc
    44221 ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca gccaactatt
    44281 gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt cagcttcacc
    44341 gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat aatacggctc
    44401 ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg
    44461 aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac cggttaatac
    44521 aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct gcaactgcaa
    44581 tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca cctctgccag
    44641 ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt ccacgctctc
    44701 atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg cttcggcgtc
    44761 gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc cagcataagg
    44821 aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca ttgtaccacc
    44881 accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata ccgaataact
    44941 tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt caaccgcttt
    45001 gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg
    45061 cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg catactcttg
    45121 cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg cggtatggac
    45181 gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca taccaatttc
    45241 attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga tctgacaatc
    45301 tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc
    45361 gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa tgatattgac
    45421 cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac caatttccag
    45481 tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt
    45541 tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc tccagagtca
    45601 taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc
    45661 ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc aagattgagc
    45721 ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc cgcttccttc
    45781 agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg caacacttca
    45841 ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt gcgatctggc
    45901 ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca ccgcgcaatc
    45961 ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct gacgctcacc
    46021 tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac atcctgggtc
    46081 ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc attacgcgtg
    46141 taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc atcttccatc
    46201 cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag caggaaaagg
    46261 atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc gctgtttcgc
    46321 gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta ctaccaactg
    46381 cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc tggtcatacc
    46441 cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca taacgtcaat
    46501 acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt
    46561 ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat cagagagata
    46621 cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg gtaaaatttc
    46681 ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa tcaatacttc
    46741 ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg ctactacttt
    46801 tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca aacccagagc
    46861 agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc gggttaacgc
    46921 tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc gtagtttatc
    46981 tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca tcagctcgag
    47041 catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa acactttctg
    47101 aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgatag gatacgcatc
    47161 aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag aaacttcccg
    47221 ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt tcttcctccg
    47281 gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg atattaccag
    47341 ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc tcggctacct
    47401 gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt gtgcgacaca
    47461 gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact tccagcatca
    47521 acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg cgcgtacggg
    47581 taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct gcgcgcgccg
    47641 ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc agcattgccg
    47701 taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca aaacggcgac
    47761 cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg
    47821 tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc gcattcaggg
    47881 tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc gggccaatct
    47941 gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt gtggtctgac
    48001 gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc accgcacgac
    48061 cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc tcaaagtcgc
    48121 cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc atcggttgca
    48181 gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga ccatgcccca
    48241 aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc
    48301 ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg tgcgttcctg
    48361 caatggcgat gccatgacga aaacgcatta actcagccag catttcggca cgacggatca
    48421 ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa atggcgctgg
    48481 aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg
    48541 cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa ccactgatct
    48601 gataaccttc attggccaga acttcggcaa taccgcccat accggcacca ccaatgccga
    48661 caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt tttgccaatt
    48721 gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca aaaggcatcg
    48781 ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg tggcatccgg
    48841 aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg accacccggc
    48901 cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcaataattt tggctgcgcc
    48961 cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt gaaacggcac
    49021 aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac cggagcggca
    49081 aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt cagtcacttt
    49141 atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg aacctttgcc
    49201 gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg
    49261 gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg gaccttcacg
    49321 tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca ccgggttacc
    49381 caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg tagcaatctt
    49441 cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta caaccggaat
    49501 gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca taccgagtac
    49561 cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg
    49621 cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga tacgaatgaa
    49681 atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg cagtccccag
    49741 ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc ccgggaatac
    49801 atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa
    49861 cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg caacagcatc
    49921 atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag cggcaatgtc
    49981 agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta
    50041 aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat ttctaatgct
    50101 ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag tgctagtacc
    50161 acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc
    50221 ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag ttcgccgcga
    50281 ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc ttcccacggg
    50341 ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag caacacaacc
    50401 gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc cgccaggaac
    50461 aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg ctgtgccagc
    50521 agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg caggttatta
    50581 cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag cgacagtttt
    50641 gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga tgccccttta
    50701 acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag cagcatcgtg
    50761 gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc cagaataaac
    50821 gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt
    50881 tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc gaggccgaag
    50941 gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt ttcccgcgag
    51001 cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa tcctggcagg
    51061 cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc gccagacggg
    51121 caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca caggccgggg
    51181 agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata gcctgttcca
    51241 tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg ccgtcacgac
    51301 cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg ctaaagtccg
    51361 ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc aggccattca
    51421 gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag cgcacaccgt
    51481 tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt aacgctttca
    51541 ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc agcgcattgg
    51601 tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc tcgcctttaa
    51661 cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg ttgacgccaa
    51721 agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca tcagcattaa
    51781 ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac tgttgtaaac
    51841 caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct gctaccgcct
    51901 gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac agttcacact
    51961 catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca acgttaaccc
    52021 ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg ttagaaccgg
    52081 taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg ccaacgattt
    52141 cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata ccgggactgg
    52201 cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg tggcgttcta
    52261 cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata acgcgcggcg
    52321 tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc aggccgataa
    52381 tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg ttgccagacc
    52441 aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg gttccggcca
    52501 gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag
    52561 tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca cgccccccat
    52621 aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg caccacctaa
    52681 cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa accacaggaa
    52741 gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg cgtgtcgcag
    52801 atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg ccaccagcgc
    52861 aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac catcggtcag
    52921 gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt agaacagccc
    52981 cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt
    53041 gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc agaaatattt
    53101 ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat catcaacaaa
    53161 gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg gattggacgg
    53221 gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc cgcccatggt
    53281 cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta cctgaccaaa
    53341 ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga tgaacagcgc
    53401 ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa agccggaata
    53461 atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg taaagcgcgt
    53521 actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat tacctgttgc
    53581 tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca
    53641 acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac gcggtcaata
    53701 ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc agcgcccagt
    53761 tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag tacctgaaca
    53821 gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa ctggttttct
    53881 gccagttgga tggggaacag acggccagga acagctttca gatttgccag ccccgctttg
    53941 atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag cgcattcgca
    54001 atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg ggtttgtagg
    54061 gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc gctgttggcg
    54121 gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca gttcagccag
    54181 tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct aaagatttca
    54241 cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg
    54301 ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat ctcgccctga
    54361 tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg caacagcgtc
    54421 atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt gccgcactgg
    54481 cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc cgtcagagca
    54541 accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa cgccagacgc
    54601 gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac cagtagtgcg
    54661 cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg ttcacctttc
    54721 agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac agcatcaagg
    54781 gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct aagggttacg
    54841 ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag tagtccagac
    54901 gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt accacatcat
    54961 tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc atcactttgg
    55021 catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc ggttcttcgg
    55081 tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca attgcgccca
    55141 tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac cacagcttgc
    55201 ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt
    55261 aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg
    55321 gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc gccagcagtg
    55381 ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg ctttcaattt
    55441 cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga tagttcactt
    55501 cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc attgataccg
    55561 caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg gcgttaataa
    55621 tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg tagtgttcca
    55681 tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac gccggaaatt
    55741 tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt gcgcaaaaag
    55801 tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc gaaccggtag
    55861 tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc attaccgcgc
    55921 tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg gttttgccgt
    55981 tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg
    56041 ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg ccgtgcattt
    56101 cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca gccacacctt
    56161 gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact acagctacaa
    56221 agagatcgcc cgccgcagcc acacagctgt cgagtgtcat ctctcgcagt gctcgcgaag
    56281 gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc acctgtcccc
    56341 tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc cggctcgatg
    56401 ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga aacggcgccg
    56461 ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa gcgcggctga
    56521 ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg accgtccggc
    56581 ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat cgccgccttc
    56641 acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt gcgaacaatg
    56701 gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat cgacagtggg
    56761 cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa cggtgttacc
    56821 attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg ttgtttttga
    56881 ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc cagtccaaaa
    56941 cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt ggaaacaccg
    57001 acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg tgccacgtct
    57061 ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt ttcccgcacc
    57121 acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc cggttcaaac
    57181 acgtcggtga tggtacggtt acgcatcgac tctttcggcg tgccgctcag attgttaggg
    57241 ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc caccagcacg
    57301 gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg ataaaccagc
    57361 gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct gtcagtagaa
    57421 gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc acccggctgc
    57481 ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact atcgacgtta
    57541 gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg agactcttca
    57601 cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc agagtttacc
    57661 tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc agaaagctga
    57721 tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac gctgataccg
    57781 ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac gctcaccgct
    57841 aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg ctgaacgcga
    57901 agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg taaccacgct
    57961 acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa cgcaaaacgc
    58021 caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt cgccgctgct
    58081 ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga cggatcaaca
    58141 tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc gccgagcgca
    58201 ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc cagcaccagt
    58261 tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac ggtcaccgcc
    58321 gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa aagatcgtca
    58381 ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt aactttgctt
    58441 agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc agaactgaac
    58501 tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg cctagtgctc
    58561 gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac cctgccggaa
    58621 cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct tccagcgagt
    58681 ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag ctttttagcg
    58741 cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg aaggtacggg
    58801 tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact tccgccagtt
    58861 ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg gcaatgcgtt
    58921 tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct tcttctgcgg
    58981 tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg tccagcggac
    59041 catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa gagacgccaa
    59101 gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg tattcgccca
    59161 gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc ttcgcaacgg
    59221 cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca agctgcgaga
    59281 ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg ccatcaggac
    59341 ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta tagttttcca
    59401 tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc caactgctct
    59461 gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag ctcaaacttg
    59521 ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg cagtactggc
    59581 gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc taacagtagg
    59641 cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt ttgctcgata
    59701 atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc aatggtgcaa
    59761 accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg cactgataag
    59821 cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc ctcacccctt
    59881 attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga ggaaaagctt
    59941 gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc taatatcagc
    60001 gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc tgtaagaaaa
    60061 aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc aaatataaag
    60121 tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa cagtccttgt
    60181 tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa agtgtttgtt
    60241 aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg tcgatatcac
    60301 ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa accatttatc
    60361 aataatagtc aggaatagtc ttatttactt taaggatatt gatg
//
LOCUS       DBKELA010000011        58051 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.011, whole genome shotgun sequence.
ACCESSION   DBKELA010000011 DBKELA010000000
VERSION     DBKELA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.011"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(27..281)
                     /locus_tag="ACUQR0_000666"
     CDS             complement(27..281)
                     /locus_tag="ACUQR0_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HHV9107836.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            436..1008
                     /gene="yeiP"
                     /locus_tag="ACUQR0_000667"
     CDS             436..1008
                     /gene="yeiP"
                     /locus_tag="ACUQR0_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HHV9107837.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1231..2697
                     /locus_tag="ACUQR0_000668"
     CDS             1231..2697
                     /locus_tag="ACUQR0_000668"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HHV9107838.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2815..3801
                     /gene="yeiR"
                     /locus_tag="ACUQR0_000669"
     CDS             2815..3801
                     /gene="yeiR"
                     /locus_tag="ACUQR0_000669"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HHV9107839.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3840..4553
                     /gene="lpxT"
                     /locus_tag="ACUQR0_000670"
     CDS             3840..4553
                     /gene="lpxT"
                     /locus_tag="ACUQR0_000670"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HHV9107840.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4965..5531
                     /gene="mepS"
                     /locus_tag="ACUQR0_000671"
     CDS             4965..5531
                     /gene="mepS"
                     /locus_tag="ACUQR0_000671"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HHV9107841.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5712..7268
                     /locus_tag="ACUQR0_000672"
     CDS             5712..7268
                     /locus_tag="ACUQR0_000672"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HHV9107842.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7350..9164
                     /locus_tag="ACUQR0_000673"
     CDS             7350..9164
                     /locus_tag="ACUQR0_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHV9107843.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9165..10259
                     /locus_tag="ACUQR0_000674"
     CDS             9165..10259
                     /locus_tag="ACUQR0_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HHV9107844.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10259..11284
                     /locus_tag="ACUQR0_000675"
     CDS             10259..11284
                     /locus_tag="ACUQR0_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9107845.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11286..12875
                     /gene="yejF"
                     /locus_tag="ACUQR0_000676"
     CDS             11286..12875
                     /gene="yejF"
                     /locus_tag="ACUQR0_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HHV9107846.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12879..13223)
                     /locus_tag="ACUQR0_000677"
     CDS             complement(12879..13223)
                     /locus_tag="ACUQR0_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HHV9107847.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="ACUQR0_000678"
     CDS             complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="ACUQR0_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HHV9107848.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="ACUQR0_000679"
     CDS             complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="ACUQR0_000679"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HHV9107849.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15619..17379
                     /locus_tag="ACUQR0_000680"
     CDS             15619..17379
                     /locus_tag="ACUQR0_000680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHV9107850.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17504..17788
                     /gene="rplY"
                     /locus_tag="ACUQR0_000681"
     CDS             17504..17788
                     /gene="rplY"
                     /locus_tag="ACUQR0_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HHV9107851.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="ACUQR0_000682"
     CDS             complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="ACUQR0_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HHV9107852.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19116..19343
                     /locus_tag="ACUQR0_000683"
     CDS             19116..19343
                     /locus_tag="ACUQR0_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HHV9107853.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19363..21123
                     /gene="yejM"
                     /locus_tag="ACUQR0_000684"
     CDS             19363..21123
                     /gene="yejM"
                     /locus_tag="ACUQR0_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HHV9107854.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21198..21274
                     /locus_tag="ACUQR0_000685"
     tRNA            21198..21274
                     /locus_tag="ACUQR0_000685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21232..21234,aa:Pro,seq:ggg)
     gene            complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="ACUQR0_000686"
     CDS             complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="ACUQR0_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HHV9107855.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24288..24935
                     /gene="narP"
                     /locus_tag="ACUQR0_000687"
     CDS             24288..24935
                     /gene="narP"
                     /locus_tag="ACUQR0_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HHV9107856.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="ACUQR0_000688"
     CDS             complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="ACUQR0_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HHV9107857.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="ACUQR0_000689"
     CDS             complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="ACUQR0_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HHV9107858.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="ACUQR0_000690"
     CDS             complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="ACUQR0_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HHV9107859.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="ACUQR0_000691"
     CDS             complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="ACUQR0_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HHV9107860.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="ACUQR0_000692"
     CDS             complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="ACUQR0_000692"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HHV9107861.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="ACUQR0_000693"
     CDS             complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="ACUQR0_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HHV9107862.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="ACUQR0_000694"
     CDS             complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="ACUQR0_000694"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HHV9107863.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30809..31432)
                     /gene="ccmA"
                     /locus_tag="ACUQR0_000695"
     CDS             complement(30809..31432)
                     /gene="ccmA"
                     /locus_tag="ACUQR0_000695"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HHV9107864.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31445..32047)
                     /gene="napC"
                     /locus_tag="ACUQR0_000696"
     CDS             complement(31445..32047)
                     /gene="napC"
                     /locus_tag="ACUQR0_000696"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HHV9107865.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32057..32506)
                     /gene="napB"
                     /locus_tag="ACUQR0_000697"
     CDS             complement(32057..32506)
                     /gene="napB"
                     /locus_tag="ACUQR0_000697"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HHV9107866.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32503..33366)
                     /gene="napH"
                     /locus_tag="ACUQR0_000698"
     CDS             complement(32503..33366)
                     /gene="napH"
                     /locus_tag="ACUQR0_000698"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HHV9107867.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33353..34048)
                     /gene="napG"
                     /locus_tag="ACUQR0_000699"
     CDS             complement(33353..34048)
                     /gene="napG"
                     /locus_tag="ACUQR0_000699"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HHV9107868.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34055..36541)
                     /gene="napA"
                     /locus_tag="ACUQR0_000700"
     CDS             complement(34055..36541)
                     /gene="napA"
                     /locus_tag="ACUQR0_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HHV9107869.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36538..36801)
                     /gene="napD"
                     /locus_tag="ACUQR0_000701"
     CDS             complement(36538..36801)
                     /gene="napD"
                     /locus_tag="ACUQR0_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HHV9107870.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36791..37285)
                     /gene="napF"
                     /locus_tag="ACUQR0_000702"
     CDS             complement(36791..37285)
                     /gene="napF"
                     /locus_tag="ACUQR0_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HHV9107871.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37394..37558
                     /gene="yojO"
                     /locus_tag="ACUQR0_000703"
     CDS             37394..37558
                     /gene="yojO"
                     /locus_tag="ACUQR0_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HHV9107872.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37672..38181
                     /gene="eco"
                     /locus_tag="ACUQR0_000704"
     CDS             37672..38181
                     /gene="eco"
                     /locus_tag="ACUQR0_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HHV9107873.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(38331..39943)
                     /gene="mqo"
                     /locus_tag="ACUQR0_000705"
                     /pseudo
     CDS             complement(38331..39943)
                     /gene="mqo"
                     /locus_tag="ACUQR0_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(40161..41804)
                     /gene="yojI"
                     /locus_tag="ACUQR0_000706"
     CDS             complement(40161..41804)
                     /gene="yojI"
                     /locus_tag="ACUQR0_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HHV9107874.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41880..42530)
                     /gene="alkB"
                     /locus_tag="ACUQR0_000707"
     CDS             complement(41880..42530)
                     /gene="alkB"
                     /locus_tag="ACUQR0_000707"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HHV9107875.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42530..43594)
                     /gene="ada"
                     /locus_tag="ACUQR0_000708"
     CDS             complement(42530..43594)
                     /gene="ada"
                     /locus_tag="ACUQR0_000708"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HHV9107876.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43668..44723)
                     /gene="apbE"
                     /locus_tag="ACUQR0_000709"
     CDS             complement(43668..44723)
                     /gene="apbE"
                     /locus_tag="ACUQR0_000709"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HHV9107877.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44835..45956)
                     /gene="ompC"
                     /locus_tag="ACUQR0_000710"
     CDS             complement(44835..45956)
                     /gene="ompC"
                     /locus_tag="ACUQR0_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HHV9107878.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46694..49366
                     /gene="rcsD"
                     /locus_tag="ACUQR0_000711"
     CDS             46694..49366
                     /gene="rcsD"
                     /locus_tag="ACUQR0_000711"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HHV9107879.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49383..50033
                     /gene="rcsB"
                     /locus_tag="ACUQR0_000712"
     CDS             49383..50033
                     /gene="rcsB"
                     /locus_tag="ACUQR0_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HHV9107880.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50233..53082)
                     /gene="rcsC"
                     /locus_tag="ACUQR0_000713"
     CDS             complement(50233..53082)
                     /gene="rcsC"
                     /locus_tag="ACUQR0_000713"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HHV9107881.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53357..54133)
                     /locus_tag="ACUQR0_000714"
     CDS             complement(53357..54133)
                     /locus_tag="ACUQR0_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HHV9107882.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54138..55787)
                     /locus_tag="ACUQR0_000715"
     CDS             complement(54138..55787)
                     /locus_tag="ACUQR0_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HHV9107883.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55788..58051)
                     /locus_tag="ACUQR0_000716"
                     /pseudo
     CDS             complement(55788..58051)
                     /locus_tag="ACUQR0_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14199 a        15658 c        14265 g        13929 t
ORIGIN      
        1 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
       61 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
      121 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
      181 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
      241 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
      301 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
      361 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
      421 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
      481 aacggcaaac tgctgctggc taaggatatt gatattcagt cgcccactgc ccgcggtgcc
      541 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
      601 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
      661 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
      721 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
      781 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
      841 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
      901 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
      961 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
     1021 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
     1081 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
     1141 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
     1201 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
     1261 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
     1321 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
     1381 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
     1441 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
     1501 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
     1561 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
     1621 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
     1681 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
     1741 cgccgccgtg agcgcagcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
     1801 aatggccatg tggtgaaaaa cgcggtgctg agaatggcag aaaaacgttc gccagaactc
     1861 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
     1921 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
     1981 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
     2041 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
     2101 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
     2161 ttcgcctata tcagtgattg catgcaggat cgcgtatttc gctatgctgc cagaacatta
     2221 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
     2281 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
     2341 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
     2401 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
     2461 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
     2521 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
     2581 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
     2641 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
     2701 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
     2761 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
     2821 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
     2881 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
     2941 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
     3001 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
     3061 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
     3121 gatctgttaa ccgcaccagt ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt
     3181 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
     3241 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
     3301 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
     3361 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
     3421 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
     3481 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
     3541 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
     3601 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
     3661 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
     3721 gagctgattt ccagcagcga agctgactgg aatgccttgc agagcgcgct gttgaagctt
     3781 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
     3841 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggccccgcg ctgtttcttt
     3901 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
     3961 tctttaatca gaaactggtc gaaagtaagg cctttttatg gctggttgca ttgactaaca
     4021 atcgcgcctt cgacggttgt tcactgctgg cgatgggtat gttgatgctg agtttctggc
     4081 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
     4141 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
     4201 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
     4261 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
     4321 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
     4381 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
     4441 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
     4501 ccttttttga caaatcacta ccaggaaaaa acaaacattt ccaaaacaaa taactcacag
     4561 taattaacat catcagggtt atttttatag tgaggataat cctgatgatg cgcaccgtgc
     4621 tttcatctat cgaacgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
     4681 ctcaattgta ctttaattga ccaaccccgc ttattaactt tctgtatcac tttttcttat
     4741 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt
     4801 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
     4861 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt
     4921 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
     4981 cgattttgag atatatcttg cgcgggattc ccgcgattgc agtagcggtt ctgctttctg
     5041 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
     5101 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
     5161 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
     5221 gcagcactaa aaaaggtatc gattgttctg gtttcgtaca gcgtacattc cgtgagcaat
     5281 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
     5341 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
     5401 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
     5461 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
     5521 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
     5581 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
     5641 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
     5701 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
     5761 ttgtagcagg cgtgatgatt gcgatactgg gtagctgcct tcagttttta gtggcctggc
     5821 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
     5881 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
     5941 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
     6001 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
     6061 tcaatgaatt gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg
     6121 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
     6181 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
     6241 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
     6301 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
     6361 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
     6421 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctt tgctattacc
     6481 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
     6541 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgggtaacg ggcctggaag
     6601 tactgctacg ctggcggcat cctgtagcgg gagaaattcc cccggatgcc ttcattaact
     6661 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
     6721 gcgatgccgc agaattagaa aaagtactgc cggtaggcgt caaatttggc attaacattg
     6781 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
     6841 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
     6901 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
     6961 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
     7021 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
     7081 acgcggtgct gacgctggcg aaacgtctca atatgctgac agttactgaa ggggtcgaaa
     7141 cgccggaaca agcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
     7201 ttagtcaccc gttaccgctg gacgattttg ttcgctggct gaagaaaccg tatacgccgc
     7261 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
     7321 aatgatgagt aaaaggaaat ccgttgcaga tgattgtgcg catactgctg ctgtttatcg
     7381 ctctgttcac cttcggtgcg caggcgcagg ctatcaagga aagctatacc tttgccgtgc
     7441 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
     7501 caaaaggtgg gcagataacg ttgtcagccc tcggtacctt cgataatttc aaccgctatg
     7561 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgtta tttacgactt
     7621 ccgatgacga accaggcagt tattacccgc tgattgctga aagtgcacgc tatgctgacg
     7681 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttctccca
     7741 ttactgcccg cgatgtagag tttacttttc aaaaatttct gaccgaaggc gtgccgcaat
     7801 ttcgtctggt ctacaaaggc accaccgtca aagccattgc gccgttaacc gtgcgcattg
     7861 agttagctaa acccggcaaa gaagatatgt tgagtctgtt ttcgctgccg gtatttccag
     7921 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
     7981 gtccgtacca cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
     8041 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
     8101 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
     8161 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
     8221 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
     8281 cgtttaatat ccaacgtgcg gtattcagcg atcgccgggt gcgggaagca atcactctcg
     8341 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
     8401 gttattttca aaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
     8461 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
     8521 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
     8581 acgaagcggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
     8641 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
     8701 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
     8761 ctaaccgcat gcgcaatcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
     8821 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
     8881 cccccggcgt gcaaagcccg gttatcgact cactgatcaa ccaaattatt gccgcgcagg
     8941 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
     9001 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
     9061 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
     9121 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
     9181 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
     9241 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
     9301 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
     9361 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagat ccagaagtga
     9421 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
     9481 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
     9541 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
     9601 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
     9661 ttgacgtctg gagtagcaca tttatcatca tcggctacgc cattccggcc tttttgtttg
     9721 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
     9781 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
     9841 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
     9901 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
     9961 aaggggtaag tgagaaaaac attctctgga aacatatgtt ccgcaacgcc atgctgctgg
    10021 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    10081 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    10141 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    10201 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    10261 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    10321 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    10381 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    10441 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    10501 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    10561 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    10621 ctggctggga acggatgcaa acggcggcga tgtgctggca cgcattctct atggcacgcg
    10681 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    10741 gggggcgcta caaggctatt acggcggtaa agtcgatctc tgggggcaac gctttattga
    10801 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    10861 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    10921 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    10981 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    11041 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    11101 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    11161 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    11221 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    11281 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    11341 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    11401 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    11461 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    11521 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    11581 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    11641 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    11701 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    11761 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    11821 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    11881 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    11941 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    12001 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    12061 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    12121 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    12181 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    12241 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    12301 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    12361 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    12421 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    12481 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    12541 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    12601 cattaattct taagccctcg ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    12661 cggtacaggc gcaaatattg acgcttttga aatcattgca acaaaagcat caactggcct
    12721 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    12781 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc
    12841 aggagtatac gcgtcagtta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    12901 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    12961 tggcgattta caaacaggca cggctcacct tcacactcca acaccgaaca tggaacttcg
    13021 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    13081 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    13141 tcaaccttcg aacctgcgaa accagcagac cagcgatagt tctgcggcag tcgatggacg
    13201 attgagagtt gtaatgaagt cacttatttt ccccggaagt acattcactt cacaatttgt
    13261 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    13321 cctcgcctgc gtggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    13381 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    13441 gactttttac ataaattgat tttacataaa ataaacatat atcggggaaa tgatagattt
    13501 gtggttgaca tgcatcaaca aaggaagcct tttaccttcc tcgttgtgca atagatcacc
    13561 gttttttcgg ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    13621 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    13681 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    13741 gaaactcatc aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa
    13801 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcggcgca ctgatgacca
    13861 tccacgcagc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    13921 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    13981 caaagtaata accaaagttt tccggcgcga cgtggttaat ttcaatataa acaaacggtc
    14041 cggcgcttaa gaatgagaac atcccggcaa agctgaaacc actggcaagc atgtagctca
    14101 ggacacgttt atggcggaac agcgccgcaa agttaccaat agtggtacga atgtaaaatg
    14161 gctgacgacg ctccggtggt aaggtttctt taatcaggaa gaaaatcatt gccgaagcca
    14221 gaatcgccgc taatgccagg atccagaaga tgtaatgcca gctcagccac accagcaccc
    14281 agccgccaac tatcggtgcc atcagcagtg caatggtcgt caccagcatg acaaacgaca
    14341 tcatccgcga gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc
    14401 tggccgcagc cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt
    14461 gtgccaacgc acacgccacc gcggcggcgg caaacaccag cgtcccgcca agaaccaccg
    14521 gcttacgccc gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca
    14581 gaatataagt actgagggtc atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa
    14641 ttaccggtag cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc
    14701 caaggataaa aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa
    14761 agtcattgaa aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac
    14821 cgggggctaa atcagcatcc agcgtaatac cgccaatacg ttcacgatgc agctcaacca
    14881 cgtggttacc cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg
    14941 tcagacgaac ctgcgttggg gtaatcactt caagcaccgc cggcttagtg agatcttttt
    15001 cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acaggtgatt
    15061 ccagtgtcac cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc
    15121 actgaccatc atcagtcatc agcaccagac cggtggtatc gatatccaga cgtcctgcgg
    15181 cgtgcagttt ccacgctacc ggttcatcca gaaaatagag caccgtcggg tgatccgggt
    15241 cgtccgtgga gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt
    15301 gctgcgccag cgggttgcca tcgtaagcga catcatgttc agggagcagt ttgaacgctg
    15361 cattacgaac gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa
    15421 tagcacggct aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt
    15481 tgcctgtaaa aaacggaggt cgggtaatga cccgaaaatc tgaactgttg tctgcccagt
    15541 atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata
    15601 atctacctac gcgaaaccat gatttttaca ctccgcccat atcagcaaga agccgtggat
    15661 gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggc
    15721 gcaggtaaaa gcctggtgat agcggaactg gcacggctgg cgcgtggtcg cgtactggtg
    15781 ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg
    15841 gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa agtggtattt
    15901 ggcagcgttc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg
    15961 attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc
    16021 actcacctga ctaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt
    16081 cgattgggca aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag
    16141 aaagcccttt tccgtgactg catttatgag ctgccgctgc gttatatgat taaacacggc
    16201 tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg
    16261 caggcacaga gtaacgggct gttcagcgaa gctgatctca accgtgagct gaaaaaacag
    16321 caacgcatta cgccacatat catcagccag atcatggagt tcgccgaaaa gcgcaaaggg
    16381 gtaatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc
    16441 gaagatgctg cactgattac tggcgacaac cccggcgctg agcgcgatgt gttaattgaa
    16501 gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cagtactgac caccggattt
    16561 gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ctgaatcagt gagtctttac
    16621 caacaaattg tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt
    16681 gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa
    16741 agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg
    16801 gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg
    16861 tttgaagatg acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc
    16921 ccgcaatgta acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta
    16981 ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta
    17041 cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc
    17101 acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc
    17161 cagcgtaccg ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct
    17221 ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat
    17281 tttgtcgtcg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac
    17341 gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat
    17401 aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg
    17461 ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc
    17521 agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt
    17581 cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa
    17641 agtcatgaac atgcaagcta aagctgaatt ctacagcgaa gttctaacca tcgttgttga
    17701 cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct
    17761 gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg
    17821 gcggggtttt ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc
    17881 agataaggcg ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag
    17941 acgtccggcg ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat
    18001 cggttgctgg atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca
    18061 agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga
    18121 agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta
    18181 cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct
    18241 cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg
    18301 cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttca
    18361 atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc
    18421 ctttcaggaa agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga
    18481 tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat
    18541 tttcgttaac gcgcatactg ctcaggttgc tcagaactgc caccagcaaa tactccaccg
    18601 ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta
    18661 attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct
    18721 gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt
    18781 tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg
    18841 gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct
    18901 ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg
    18961 gtattcaacc accgccagtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc
    19021 agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat
    19081 atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagta
    19141 atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc
    19201 cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca
    19261 ttgccccggc ccaacgccag gcaattgcca actcttttgc cagcgcctta cagtcctcta
    19321 tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag
    19381 cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat
    19441 attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg
    19501 cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt cctagtgttc
    19561 gccacctact tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg
    19621 aggtttttgt ccgtcattct ggcgacggcg ggaatgacgc tattactgat cgatagcgaa
    19681 gtctttactc gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca
    19741 gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta
    19801 ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt
    19861 cgacgcttcg cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg
    19921 gtgtatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg
    19981 ccgctttcgt acccgatgac ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg
    20041 caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgtacagtat
    20101 ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact
    20161 gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct
    20221 gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc
    20281 tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg
    20341 cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt attctcatca
    20401 gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc
    20461 gtacgcaccc aatctgacga tcagaccgcc acgcagtgga tcaactggct gggccgctac
    20521 gcacaagaag ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac
    20581 agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag
    20641 atcaaacgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtggtgatt
    20701 atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac
    20761 ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat
    20821 gctctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct gcatgtcagc
    20881 acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac
    20941 tgggttactg ctgcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg
    21001 ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa
    21061 aaaccacagt taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac
    21121 tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag
    21181 tattagcagc cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg
    21241 ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt
    21301 acggttggtt tttttatatc tgcaattaat tcgataaaca gaccgtgaca catcacagcc
    21361 tgtttatttt ctgttatcag aacgtccaga ccacacccgc ctgagtattc cacggcgatt
    21421 ctacccctgc gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg
    21481 atgaccttat cccagcctga taaacgccac tggttccagc cacatcatta ttaaatttgc
    21541 catcgtcatt aactttcacc tggttagaat cggcgtattc ctgacgcacg gcggctttca
    21601 gccagggttc cagcgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg
    21661 cttcagcgcg taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt
    21721 agtcctgacc atctgtggta aagccggtaa aggccagata gggtctaaca ctccacaatc
    21781 cgtcaaccca acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc
    21841 caaacgctgt tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt
    21901 tcaccacccc atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg
    21961 tatagctatc gatattacct tttccgccgc gatcaaaacc aatatcagaa tgagagtaac
    22021 caaagaacaa gccgcgaatt gtactgcttt cttcacggga gaaacggcta tcgataccga
    22081 gcgtcaggcc cgtcaatgtt tgctcaaaac cagctcccgc atcagtggtc acgttgttgc
    22141 gggtgttaat tgccgaactc cacatcaccg tatcgtaact aacgcctttt acgctaccaa
    22201 gacgctcacg cacggtgtcc agttctgcat caaataccag cggttgtgcg gctgccatgt
    22261 tcagcacatc agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat
    22321 ggttgccatt atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat
    22381 tgcccaacgt aaatgcagca tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg
    22441 ctgccgggct ggcaccggtg tccgtcacga atattttgaa atcacctgtt gcctgaccgg
    22501 tgacgttgag ctgatcgctc tgatgattgg ccatatcggt acgcatataa aaattgccgt
    22561 ttcccgacag ggtattggtg gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg
    22621 aacgagtcac tggctcagcc atcataatca catttgcgcc ggacatcgca aggttggcta
    22681 agttgaggcg agtgttttgg ttcgtcgtag tgatatcggc aatgatggtc catgcgctat
    22741 tttcgagcga aacgtccgct ttgctggtat ccacggcacc atgcgttcta aaagaagcgc
    22801 cctcagaaac cactacctta cccaccaaca tagcgggtgc ataatttatt tgcggcttca
    22861 tgacctcaag aatgccatca ttccctctga ctgacacgtt aaccattgtg ccagcccaga
    22921 cattagcgcg gccattgttg attaccatat tttcagagat ggcttttgag gaatacgtga
    22981 tacttccatt ctggtctgat cgaccgagat catataccgc atttgcctgc actttcgtac
    23041 cagtatcctt tcccagcgat tgcatagtgg catgctcatc aaggatagtg tcaatggcct
    23101 gcgtgtcctc cataacaatg agcccggaac catcatccag ttcataattt tttgacacac
    23161 catcctttat actaaattgg cctttactgt ttgtaccact cacttccagc gcattcgttg
    23221 agacaattaa tttcccgcct gcttttttat caacgccagt tgcagtcccg ccatccataa
    23281 cctccagtaa gccgccacta tccacagtag tattataagc gaagtcattt tcttcaactc
    23341 gcaaacttcc gccgttttcc aacaacatat tattagcaat accattcttg atatcgaact
    23401 gaccgagacg gtttgttccg aatacctcca tggcccgcgt ggttgtttta atagcaccgc
    23461 ctgctttctg atctaccgca agcgcaaatc ccccctcttt aacctccagt agtcctccct
    23521 tttcgatagc ggcaaaaacg gtccccccat ctttttcaac gattaagcgc ccgcctttaa
    23581 tcgtggtatc attcgctggg ccataaacct gtagcacacc attattgaga gtagtctggt
    23641 ttgccgcgcc acccatttga acaatttgat aaccatcatt taatactgag tattctgcgg
    23701 taccattcat ttcaatgtat tgataccctc cgttaatttc agtattacta cttacaccaa
    23761 attcttttat atgctgttct ccgccaggat tgattttcgt attattggcg gtcccataaa
    23821 ccagctggat atctttttcc aggataacac catcaactgt ctcaccatac tcaactgttg
    23881 atgccaatgc tatgggtgaa tataacgctg tagccaccag aacagatatt gcggaacgac
    23941 aaagagaaac agaaccagat tgatgcattg agctttcatc ctatgaaatt aattgcagtt
    24001 aaaagcattg ggtacagaaa atacccatag ctccataccc ggagtcagtt tttaaaaact
    24061 gtttaaagaa atgcacaagt attgtgattg attttttagt tgtttttctt gatgagaagc
    24121 tgatgcaaaa ttccgtcttt ataataaaaa tgatgccaaa gcgaacgaca aggttgtagt
    24181 tttcactaca tgtccataca taaaatgggg taacattcac gcgcctggta gcgttaccaa
    24241 cgctacgctc aaacataatg attctaataa aacctcagga gactactatg cctgaagcaa
    24301 cgccttttca ggtgatgatt gtggatgatc atccacttat gcgacgcggt gttcgtcagt
    24361 tactggagct tgatcctggc tttgaagtgg tcgccgaagc gggcgaaggc gcgagcgcga
    24421 tcgatctggc gaatagactg gatatcgacg tgatcttgct ggatctcaat atgaaaggta
    24481 tgagtggcct ggataccctc aatgccttgc gcagggatgg cgttaccgcg caaattatta
    24541 tcctgaccgt atccgatgcc tccagcgatg tctttgcgct gatagacgca ggcgcagacg
    24601 gttatctgtt gaaagacagc gacccggaag tattgctgga agcgattcgt accggagcga
    24661 aaggcagcaa agtctttagc gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg
    24721 gcgcggaaga agatcccttc agcgtgctga cggagcgcga actggatgtt ctgcacgagc
    24781 tggcacaggg gctgtcaaat aaacagattg catcggtgtt gaatatttcc gagcagacag
    24841 taaaagtaca tattcgcaat ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca
    24901 ccattctgtt cctgcaacaa cgcggggcac aataaaaata gcccgatgga tttatcatcg
    24961 ggctgagatt tatgacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    25021 gatcgatcaa tttattgaat ttacacaatt ttgtaggccg gataaggcgt tcacgccgca
    25081 tccggcataa actaagcgca ctttgtcatc agtctagccc gatggcatca ccatcgggcc
    25141 tctttttatt tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttca
    25201 atcaccgcac ggcgagtatc gttggcaggt aagagtttca acatcatctc ccacgcggca
    25261 acggcttcgc caaatcgctg ctgctcaaag gcattaaacg catacatgct tagcacacgg
    25321 atattgctat ggtccgttct caccagctga cgtagcagtt caccgccgag gcggttgtcg
    25381 ttgggatcag atgaacgagt caacgcttca gcgtatccca gtgcggcatc gctgttcttc
    25441 ggatcgaggc gatacgcagt agcgtatgca tcggtggcga tactggcgtt acccagcgcc
    25501 atgccaacgc ggcccaacat aatccagcct tctatatctc ccggattttt ttgcagttga
    25561 gtacgcatcc ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg
    25621 tccagcgccc tgtccagcaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc
    25681 tgctgataat tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata
    25741 cccggcagat aaacaacata tccggcacgc ttaccttccg gcacgctttg ttcaggaaac
    25801 gcttccggca ccacgcgtac ccgacgccgc gagcgggcgt atatgaccca accgccaatg
    25861 ccaatagcca ctactggcag cacccacagc agcacggtca gcggcgttaa cggcggatcg
    25921 taagtgacga agttgccgta acgcgccacc atataatcga caatctcttt tttactttta
    25981 ccttcctgca tcagttcata cactttctga cgcaggtcgg tggcaatcat cgagttggaa
    26041 tcggcaatgc tgttgttctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac
    26101 tgttgttcct gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg
    26161 gagatcatca gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt
    26221 tctcccacag cggcttgatc ttttcttccc agacgcgagg attgagatcg cccgcatggc
    26281 gatagcgaat gataccgttg ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga
    26341 gatccagccc taacatgccg tcgccatcaa acaggcttag cgcgtaagga ttgcccagct
    26401 ctttcagcca gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgaa
    26461 tgccctgcgc tgaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccaag
    26521 tcgcccagac gttaagcagt actggtttgc cctgagtcag cacatccgcc tgataaaact
    26581 gccccggatt gtccagtgat tcgagacgaa acttcggcac aggcttgcca atgagcgccg
    26641 attccagatt ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg
    26701 cggcaatcgc caggaagata atcaacggaa ttaacaatac tttgcgcttc atacggcctc
    26761 cggcgcagtt ttttgcggac tcacgcgctt acgatagcga ggatcaaaca gacacagcag
    26821 tccgcccaac gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta
    26881 aagacgcacg gcccacgcgc cgttttccag ctcttcaccg agcgcggcgt acaggtcacg
    26941 cgtgatgccg ccgtcaattg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg
    27001 tttttccgca tacagcaccg tttccggttt accctctcgc gttacgccaa tagtcgccac
    27061 accgccgcgc cagttcgggc cagtcacctc tttgacatca cggaaggtga agcgatattc
    27121 atgaatatcg acgctatcgc cggacttcat gcgcacatca cgctcaacgc tataattctg
    27181 gctaaaggca atgccaacaa ttgtcactgc cagccccagg tgagccgcca ccatccccca
    27241 ataactgaag gtggttttcg tgccgcgtga aatacgcagc gcagcttccg cagttgccag
    27301 caccgcaatc cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc
    27361 gaacagccac ggcaacagca gagacagcac cagcgtagag atgaaggcga taatcaataa
    27421 attgcggatt ttacgtgggc gatcgcgccc ccagcgcacc agaggaccga caccaagcag
    27481 tagcgcaaac ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga
    27541 aatactgccc agtccaagtt gcttgtgcac cagcggcagc agcgtcccca gcaacaccac
    27601 cagcatcgcg gcgaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag
    27661 cgcattgttt acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc
    27721 gccaatcacc agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa
    27781 cgcgtgtacc gataccagca cgccggaacg cacgaggaaa gtccccaaca gacacaacga
    27841 gaaggcactg attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac
    27901 cgccagtgag tgcatcagtg cagtccccac cagccacggc ataaacgagg cgttttctac
    27961 cggatcccag aaccaccagc caccccagcc gagttcgtaa taggcccatg cggaaccgag
    28021 cacaatgccg agcgtcagga aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc
    28081 ataagtgctg tccagacgcc cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga
    28141 gaaacccacg taccccatat aaagcagagg cggatggaag atcagccccg gatcctgtaa
    28201 cagcgggtta agatcgcgcc cttcaatcgg gaagttcggc agcgtgcgtg agaacgggtt
    28261 agaggtaaag agaatgaaca gcaaaaagcc gacgctgacc atccccatta tcgccagtac
    28321 acgggccaca atatccagcg gaatacgctg actaaaaatc gccaccgcaa aggtccagcc
    28381 gctcatcagc agcacccaca gcagaagcga accttcatgc gcgccccaag tagccgccac
    28441 gcgataccac accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc
    28501 gttgaccacg aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag
    28561 ccaggcaaac aagcgggaag acgccatcat gcgcgcatct ccgcgcgcca cgccccatag
    28621 cggatacacg gacagcagca gcgcaatccc taacgccaag cacagcagcc cgtttccaat
    28681 ttccggcatc atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc
    28741 atcgctttct caacttctgg cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc
    28801 gcgaggatat gattgccttt ttccagctcg ccctgcacca caacgccctg cccttcacgg
    28861 aacagatccg gcaaaatgcc ttcgtaagag acatccactg agccttcagc atcgtaaatg
    28921 gtgaaggtca ctttcagcga attgggatcg cgctgcacgc tacccggcat caccatcccg
    28981 ccaacgcgca gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga
    29041 atttcccccg gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg
    29101 atagtcagcg ctagccctgc caacacggca caggcaatcc acaagcggtt tttacggcga
    29161 atattcatgc agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt
    29221 gcgccacacc acgcagaatt gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca
    29281 gcggaataac ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga
    29341 aaaattcatt ccaggaagca aatgcagggg tcatttacgg cctcttttca gtatcagttc
    29401 actcacccac ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt
    29461 cagcgtggca gacaggagca ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc
    29521 cggatcgata ctttgctgca tccgcgttga tccctgatgc agggtgttcc accactccac
    29581 ggagtaatga ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg
    29641 gcccgccaga cggcggtcgt cgaaggcgtg ccacagggca atcacaccca catacaaaaa
    29701 cagcagcacc agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg
    29761 ttttccccat gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc
    29821 catcgccgcc accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc
    29881 caccgccatt gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat
    29941 aatgcggtag ctatttccct gctgataatc agccggagca aagccgaatc cccagatcca
    30001 gccgacggta agcacgacca cactggcaat tgccagccac ggtataaacc agccacagat
    30061 ttgatacagc cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc
    30121 agactcgaac aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc
    30181 gtcgcaaaag gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac
    30241 ccatcaacgg gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat
    30301 gggatagtca gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact
    30361 gtcagcgcca cgcccggtgc accgagaaag ccaagcgtag gcgttcccag cagcagcgtc
    30421 agcgccatca cttgccagcc ataaacatcc attcccagta gcattgctac cagtggcgaa
    30481 aggatgagta acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg
    30541 gcgggcaagg gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca
    30601 cggaacagtc gttccagcgc cagcaaggat gaaagcagcg cagcaaccca gataatgccc
    30661 ggtgcaatac gcgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt
    30721 acaatcagga agaaccacag cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc
    30781 agttcaagac ggaaaatacg ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc
    30841 ggcgaatttt actttcagca acgttgagcg gctggtgggt agtcagaatc acaatccccc
    30901 cctgctccgt atgctgcgcc atacgctggg tcagacgatc gacgccgtta acgtcaatcg
    30961 tggtaaaagg ctcgtcgagg atccataatg tggcacgggt caaccacaga cgcgctaaag
    31021 cgacgcggcg ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa
    31081 gcccggcctg cgccaaggct tccagacatt gtgcggcatc gccatcgcga tgataaaagt
    31141 gcagattttc taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca
    31201 gggtttgatg gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc
    31261 cagcgtcagg gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcgccgt
    31321 tgctaccggt gatttgtacc cactctcctg cgttcagcgt aaatgacaag ccactaaata
    31381 aggttcgttc atcccgctca caaagtaact ctctggcttc aagcataccc acgcaataac
    31441 cctgttaaaa acctggctcg acttcacgca tatcgggcag cttgtgcgcg atccctttat
    31501 ggcaatcaat acaggtttgc ccatctttca ccgcctggtc atgcatcttc gcggcaaccg
    31561 atttctgggc ggttgtatcc atatactcga agttgtgaca gttacggcac tcctgcgagt
    31621 tattgtcctt catgcgccgc cactcattct gtgccatcgt cagacgatga gcttcaaatt
    31681 tctgcggcgt gtcaataacg ccaaaaattt taccatacag ctctttactt gctttgagct
    31741 tgcgtatcat cttcggcaca aactcgtgcg gaacgtgaca atccggacag gtcgcacgga
    31801 cgccgctacg gttgttgtag tgcacggaat ccatgtattc ctgatacacc gtgttgcgca
    31861 tttcgtggca gctaatgcag aactcttcgg tattggcttt ttccatcccg gtgttaaagc
    31921 ccccccagaa gacgatgccg ccaacaaaac cgatcaacag cagcgtcccc agcgccagat
    31981 ggctgggggt acgccaccat ttccacaggc gcttaatcag accaggctta cggtcagaat
    32041 ttcccataat aacctcttat ttcccgtaac cttttgatgg ggtaaaggta ttccccacga
    32101 ttggcgcggt atcggcctgc ggtacgtgac attgcagaca gaaataacga cgcggagcca
    32161 cttccgcgcc cactttgccg tcgctgtcca taaagtgagt aggactgata cgcggcgcgc
    32221 cagtggtgcg atagctttcg acaccgtggc actgcaagca gcgattggta ttggtcgtta
    32281 cctgataacc ttcaacgcta tgcgggatca ttggcggctg attcacatag ttcagcggca
    32341 tccgatcctg ctcttttggc atccgaatgg ccccttcctg cgtcccggat acttccggcg
    32401 actggctaaa atcgacgcca ttagccgccc aaaccgcgcc gcttaccacc agggccagca
    32461 tcgccgtcca ttgacacagc gctttcttca ggtcatggct tttcatgatt tcgctcccga
    32521 actccatcgt gtagttattg taaatacatc ctcagaacag acatccacgc agcgaccgca
    32581 cgtcatgcaa tcgcggctgg tgacctgcac cgggctttgt tcatccagca ccggggcacg
    32641 tagcacatgc ggttccgggc aaacatgaaa acaatccata cagcggttac atttctgccg
    32701 atcgctggcc gcaacggtaa tcacaccttt gctacccagc acgccataca gcgcgccaac
    32761 cggacaaatg tgcccgcacc agccgtgttc aacgaccagt agatcaaata aaaacagcgc
    32821 gagaataagc agcgcgccgc tgccgaagcc cattaccagg ctacgcccca tcagagaaac
    32881 ggggttgatc cattcccaaa tgagcgtgcc agttaaggct gatcccacca gaatgaccac
    32941 caacagcacg tagcgaatgt ggcgagggat cgtcgcagac tgattgaggt caaagcgcct
    33001 gcgtaaccag ttcgctaagt cggtaatcgg gttcagcggg cagacccagc tgcaaaataa
    33061 tcgctttccc gccagggcgt agagcacggt gataatcacc gcaccggtca acgccaccgt
    33121 ggcaggcaga tgaccactgg caagactttg cagcgtcatc agcggatcgg tcagcggaac
    33181 ggtgtcgaat aacaggctac tgctgtagtt gccgtgcaag atccacacac caaaccatgg
    33241 accgctcaaa aacatcccca gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg
    33301 gtgactgcgc caccagcctt ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt
    33361 tgccattgtt cccctccagc cagccgaagc ggtaatggtg acctaactcc cctttcgcca
    33421 gtgacagcgg taacaccttg attgccggtt gttccagcac gcatactttt tcgcacttac
    33481 cgcaaccggt acaggcgtcg ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac
    33541 gcgtgttgcg ctccagctcc aaggtgatgg cctcatcaat tttcgggcat tcgcgataac
    33601 aaacatcgca gcgcagcccc tgaaagttga gacagttttc ctggtccacc agtaccgcca
    33661 gccccatccg cgcgtcgtcg atcgattcaa tctcacgatc cagcgcaccg cttgggcaca
    33721 ctttggcgca cggaatgtcc tcacacattt cgcaaggaat atcccgtgcg acaaaatatg
    33781 gcgtgcccgc cgacagacca gaggccagcg tcgccagttt taaggtgtcg taagggcaag
    33841 cctgaacaca ctgaccgcaa cgcacacagg cactggcaaa ggcgttctcg tttatggctc
    33901 cgggcgggcg caaccgcacg ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca
    33961 cacccacggc agccagcccg cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac
    34021 cattttgagg tttcgctgac cgggacataa tggcttacac cttctccagt ttgaccgcgc
    34081 acttcttgaa gtccgtctct ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa
    34141 ccagctgtgc ggcgtcgaag aacggcatgt acaccaggcc ctgtggcgga cggttacgac
    34201 cgcgcgtttc aacaatcgag atcacttcgc cacggcgaga aaccactttc actttgtcgc
    34261 cacggcgcag atcgcgcgct ttcgcatcca gcgggtgaat aaacaagacc gcttccggga
    34321 aggcgcggtg cagttccggc acacggcgag tcatactgcc ggtgtgccag tgctccagaa
    34381 cgcgtccggt agagagccac aggtcgtact cttcatccgg tgcttccgcc gccggttcga
    34441 acggcagtgc gaagatcacc gctttgccat ccggtttacc gtagaactta tagccttcgc
    34501 ccgctttcac gtacgggtcg ttaccttcgc tgtaacgcca ctgtgtttct ttaccattca
    34561 ccaccggcca gcgcagaccg cgcgctttgt ggtagtcatc gaacggtgcc agatcgtgac
    34621 cgtgaccgcg accaaaccat gcgtactctt cgaacagccc tttttgcaga tagaagccca
    34681 gctcgcggga ttcatcgttc agctgatctt ccgccagttc ggataccggg aatttgctca
    34741 cttcgggggt ggcatacaga acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg
    34801 ccagcagctc ttccggccat acttcttcag ttttgaagcg gcgggagaac tggactaact
    34861 gccagagatc cgatttcgct tcgcctggcg cctgtacctg ctgacgccag aactgagtac
    34921 ggcgttcggc gttaccgtaa gcgccctctt tctctaccca cattgcggtc ggcaggatca
    34981 agtcggcggc cagcgcactg actgtcggat acggatcgga gacgatgatg aagttgcgcg
    35041 gatcgcgcca gcccggcata cgctcttcat tgatgttcgg cccggcctgc atgttgttgg
    35101 tacacatggt ccagtaaaca ttcagcttgc cgtctttcag cgcacggtct tgtgccaccg
    35161 catgaagacc gattttcgcc ggaatggtgc cgctcgggat attccacttc ttctcacaga
    35221 tatcacgatg tttctcgtta gttaccacca tgtccgcagg cagacggtga gcaaaggtgc
    35281 ccacttcacg cgcagtacca cacgcagaag gttgcccggt cagggagaac ggaccgcaac
    35341 ccggctggga aattttgccg gtcagcaggt gcaggttgta gaccaggttg ttagcccaca
    35401 caccacgggt atgctggttg aagcccatcg tccagtagga gatgactttc ttgttcggat
    35461 cggcatacag ctgcgccagt tgttctaact ggtctttcgg cacgccggtc atttcagcgg
    35521 ttttttccag cgtatactca gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg
    35581 aggcgtcaga acccggattc ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt
    35641 agccgatgtc cgtcgcccct ttgcgcaggt taacgtgctt gctgaagaag tcctgattta
    35701 tcgcattgtt ttgaatgata tagttggcga tgtagttcag gatcaccagg tcagattgcg
    35761 gcgtaaagat gatgccgtta tccgccagct cgaagctacg atgctggtag gtagaaagca
    35821 ccgccacggt gacgttctgg ttagagagac gactgttagt gatgcgtgac cagaggatcg
    35881 ggtgcatctc cgccatgtta gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt
    35941 catagcagcc catcggctca tccataccaa aggtacgcat aaagccaact actgccgacg
    36001 ccatacagtg acgtgcgttc gggtcgatgt tgttcgaacg gaagcccgct ttgaacagct
    36061 tggacgcggc ataaccttcc cagatagtcc actgaccaga accgaacata ccgatcgatt
    36121 ccggcccttt ttctttcagg gcggttttga atttctcttc catcacatcg aaggcctgat
    36181 cccaggtgat tggggtaaat tcgccttctt tgtcatattt accgtttttc atacgcagca
    36241 gcggctgcgt caaacggtct ttaccgtaca tgatcttggg caggaaatag cccttaatgc
    36301 agttcaggcc acggttaacc ggtgcgtccg ggtcgccctg acaggccacc acacgcccct
    36361 gctgcgttcc gaccagaacg ccgcaaccag taccgcagaa acggcacggc gctttatccc
    36421 atttaatggc ttcctgctga ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg
    36481 cagccgccgc agcggccgca acggcgttag ctttcataaa gctacgacga ctgagtttca
    36541 tggtgtttcc tcaccttgct cttcctgctg gtgataaacc agcgacaccg ccagcacgcc
    36601 ctctacgttg cgtactgact caatggtttg gatcagcgtt tcgctgtctt ctgcttccac
    36661 caccacaatc aactgaccgc tcggcgcgtc gctgacagca acttcacagc cgggaaaggc
    36721 gttcagttgg gtgctgatgt ctgaaattcg ttcgcttttg gcctgcacga ccaggctgca
    36781 aacttgccag ttagtgtgca tggagatact ccgcagttat ggctgatacc ggacagctgg
    36841 ctgcacatgc tccacatccg ttgcaaagtt ggctattaag ttgcgcctgg tagatcccgg
    36901 agagcgtcgg gcgaaagata attgccattg gctcacagct atcctgacag cggcggcatt
    36961 caactgactg atacgccagg cacgcgtccc cgatggtgaa ctgcaaatcc caggccctgg
    37021 tgtggcgcgg agaaaatagc gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc
    37081 actcgttatt tttgaaatta acgctcggat agccacccgt gccgcgttgc agaatgttgt
    37141 tttcacaagc attaatacag gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat
    37201 caccggacca gggcggacgg ataccgttac tggctttgcg ccagcaacca gtgagtatgc
    37261 cccgacggga tgcatcaatc ttcacattga ccttccatca ttaacgcgct ctgaaaattg
    37321 agagcgacca aatagaccgc ataattaata agccattttt atagccgcta agatattaaa
    37381 ggatgtgtca aagatgcata ccccgatcgg ggtaaaacct gtagcaggat caaaagagtg
    37441 gcgggaagcg tggcaaaaac gggcttttgc tcacatttca aatggttata aacatattta
    37501 tatagcgatt aattcaccag agatatttct gctggtttgc tccctcatca gaatttaaca
    37561 ctaaaagagc aggtaaaatt gtctgaatgt tctttaagtt attcataaag caaattaata
    37621 aatctgatga atatgttaac cttcagcgac atcatcggtg aaaacctata aatgaagaag
    37681 gaaagcaaaa aaatgaagac cattctacct gaagtattgt ttgccgcttt cgctaccact
    37741 tccgcctggg cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct
    37801 gaaacaggca tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg
    37861 aaagtagaac tgttaatcgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc
    37921 gggaagctgg aaagcaaaac gctggaaggc tggggctatg attattatgt ctttgataaa
    37981 gttagctcgc cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt
    38041 gtcaccgcgt atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg
    38101 gtgtatacgc cagacaatgt agatgtgaaa taccgcgtct ggaaggcgga agagaaaatt
    38161 gacaacgcgg aagttcgcta aactgttgtg aagtacggca ccccgttggt tggataaggc
    38221 ggttacgccg catccaacat ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt
    38281 cttatcagta tacgccacat ccggcatacc atgccggatg tggcacatca ttacaacgca
    38341 atatccgcca cttctttttg aacgggttgc ggtttcggcg tactatctgc tgcttgcggt
    38401 ttgtcgtatt tcagccccag cacttcgctg gtgtactgca actcgcgttc tgttgccgct
    38461 acatcaccgt tcagcttgcg tccataagac ggaacgatcg ctttcaacgt agcctgccat
    38521 tgcgggctgg aaacgcgatc gccaaatact ttttccagta gattcaacat aatcggcgcg
    38581 gcggttgacg cccctggcga tgcccccagc agcgccgcaa tggtcccttg ctggtcactg
    38641 accacttcgg tacccagacg cagtacgcca cctttctcgg catcacgctt gataatctgc
    38701 acacgctgcc ccgcttgcca caaacgccag tcctcttttt tcgcttgcgg atagtactct
    38761 ttcaacgctt caaaacgatc ctcttcactc aacatcacct gactcaccag atatttcacc
    38821 agatcgaaat tatccagccc gacgtgcatc atcggcatca cgttagaggt ggtggtggaa
    38881 ctcatcagat cccacaatga accgtttttg aggaatttgg ttgagaaggt ggcaaatggc
    38941 ccaaacagca ctacgcgttt accgtccaga acacgggtat cgatatgcgg aaccgacatc
    39001 ggtggtgcgc caacggatgc tttaccgtaa actttcgcca gatggcgatt aaccacgtct
    39061 ggattttccg aaacaaggaa ctgtccgccc accgggaaac cggcgtagtc tttcgcttcc
    39121 ggaatccccg attcctgtaa cagcttcagc gccgcaccgc ccgcgccgat aaagacaaat
    39181 ttggcgcgga tgttctgtgc agtgccgttt ttcagatcgg caacggtaac ggtccaggtg
    39241 ttatcgtcat tacgctttag ggcgcggact tcgctgctga gttgcagcga gaagttagat
    39301 ttcttctgta aggaagcaat taactggcgg gtgatctcac catagttcac atcagtgcca
    39361 atttctgttg cggatcgcgc ccttccatca ctaacggtgc ccactctttg atctgcgcgt
    39421 gatcttcaga gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg
    39481 cgcgcaggaa attaacgtta tcctcgcccc agacaaagct catatgcgga acggtattga
    39541 taaatgaacg cggagtacgc agcacgccgc gctcgacctg gtgcgcccag aactggcggg
    39601 aaatctgaaa tgcttcgtta atggcgactg ctttttcaat actgatgctg ccatcggcgt
    39661 tttgcggggt gtagttcagt tccatcagtg cagaatgccc ggttccggcg ttgttctagc
    39721 cgttcgaact ctcctgcgcg cccccctcca ggcgctccac catggtcatc gaccattcag
    39781 gctccagctc gcgtcaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca
    39841 gtacatcagt ttcctgctcc tcggacgctt tcgcttttgc cgccatcgaa acggcattaa
    39901 gccccacggc catcgagaag agcatggcag tcactttttt catcttgtta atgccttact
    39961 tttagtcgct ttattgcagg tgagatttgc gcggcatcaa cggtaacact taagtaacaa
    40021 catttaaata atgtttataa attatattct aattttagaa acattataat ttcgttgatt
    40081 aattataggg ttattagaaa tgaagggatt tttagcatat tgacctgacg gcagcagtcc
    40141 gccaggtcag cgtcgtaata ttatgccgtc cgggcaacgg catcacgcga agcggcatcg
    40201 cgctcttcgc ccgtcagctc gctaagttgc ccattgcgca tttccagcag gcggtcggcg
    40261 tggataaagt aatgatcatc atgactgata gcgaaaatag ttttacccat ctcctgcatc
    40321 agcggcagca acacctgata aaactcacga cggaagtgtg gatcctgatc caccgcccat
    40381 tcatccagca ggataatatc gcgttcttct gccagcgcca gcaacagcgc cacgcgtttt
    40441 ttctgccctt ttgataactt caggttaaca atacgcccgt tgcttaactc aagcttatga
    40501 gccattttca gctgcgccag ccacttctca accagttgcg ggttagcggg ttgcccctcc
    40561 ggccccagca gttgatcaaa cagccagaca tcggtaaaca ctgccgaaaa cagtttgcga
    40621 taatcttccg gttgttcgcc gctgacaggt ttgccatcca gcaagatttc gccgctttgt
    40681 ggctgataca agcccgtcaa caacatcgcc agcgtcgatt ttccgctacc gttaccgcca
    40741 atcagaaaca gcagctcgcc acgtttgatg gtgagattaa tcggaccaac ggaaaacgcg
    40801 ttatcctgat aagcaaacgt cacgttacgc agctccagcg tttgccagtt gggaaacgcc
    40861 tgcgggcgcg gaaactcagc tttgaaaggc gcgagcgcga atttgttcag cttgttaaac
    40921 gccacctgcg ccgtcagcag cgtcggcaat gcgccaaccg ccgaaagcag cggcgtacgc
    40981 aggaataaca gcgtcaacga ataggtcgca gcaacgttgg tatcagccca accgagactg
    41041 ttcgccatcc agaacaccag gccgatagcg cccagcatca tgatgtttga ccagttcacg
    41101 gcactaagat ggaaggtgtc tgcgcgaata atatgatggc gatactcttg cgcatcgggg
    41161 atgtagaggt tgttaaacac atactcggcg cgttcccggt tcagagtcag ctctttgcgc
    41221 ccttcaagta cagtttgaaa atccgtgtac agcttgtctt cggtttcacg tagggtcgcc
    41281 atatgtttgt acacccgcgc caccagcaca aaaccgcccc agatggtgat cgccatccag
    41341 atagccgtta ccagcaacat tttgcccgac agcatccaca gatacgccga tgaaccgata
    41401 gtgagaatga tcccctgccc cagttccggc agacgcacaa aagcaatggt gatattgcgc
    41461 acatcgctgg ttaatcccgc cagcaacgag gcgctaccga gttgttcaat gcgctcgacg
    41521 tgggtatcca gaatccgctt gataaattca ctacgcagtc ggtaaacgaa gtgatgcccc
    41581 aaagtggtga gcgccagttg cgatccgaga gtgactgcca tcaacagcag caataatccc
    41641 agaaactccg gtaacaccag cagactggta tccgccgttt cgataaggcg ctgattgata
    41701 aaagcaatta agccaatgcc taatgccgca ctggcgaggc ttagcgccat cacactgata
    41761 aatggccagc gatactgccg ccagacaagt acaagaagtt ccatgcagaa aacccggaca
    41821 atgaattaca gcccgcagtt taaacatctt cgcgcgcaca gcaataataa ttcttatttt
    41881 tattcttttt tacctgcctg acggaatgtc aggttgtagc ggcagtcggt ggtgagtgga
    41941 tgaaaccccg ctttcaacgg ttgaataccg tgataaaaca gccgcgattc accgccccat
    42001 accaccacat cgccatgttc caacaacaaa cgtttgagcg gatcattacg tttcaggcca
    42061 ccaaattgaa aaatcgcggg taagcccaga gaaacagaaa caattggcgc gcgcagatcc
    42121 ggttcgtctt tatcctgatg cagcgacagt ttcgcgccag gagcgtagcg gttgataaga
    42181 caagcatctg gctggaaatc tggatagccc gccgccgtag ccgcacgttg acataaatca
    42241 tgaaagcttt gtggcatggc gggccacggt ttatttgttt gcggatcaat gggcgaatag
    42301 agataacctt gccgatgggt cgtccagccc agatgcccac agttggtcat cgccaccgac
    42361 atggtatatc cgcctggagc gaccatctgg cgaaacggcg actggctggc aacgtcatta
    42421 atatcgcgga ttagttgctc cgcagcatta aaagcaaaac gccgtaaaat taccgcacca
    42481 gccgccagtg gctcttgcca cggttcagca tcggcaaaca gatccaacat tacctctcct
    42541 cattttcagc ttcgcggcgc agcagttgcg ctttacgcga cacgccccag cggtaaccgg
    42601 aaagtgtgcc atcaccacgg accactcgat gacagggtat tacgatagcc agcttgttgg
    42661 cggcacaggc gctggcaacg gcccgtaccg ctttcggttt gccgatggcg ttagccagtt
    42721 gctgataact gacggtttca ccacaaggta tcgtgcgcag tgcctgccag acttgttgct
    42781 gaaaagcagt gccgcgaata tccagcggta acgtcagcgg cgtatcgcgt tgattgaggc
    42841 tggcgatcac ttcacgcaca tgttgctgaa acgtcagatc ggcaggcgcg ttgtcggcag
    42901 cgggaaacat ctgctgcaac tcgctgatta atgtcgcgtc atcatcgccc agcaatatcg
    42961 cgcaaatccc ccgctcgctt tctgccacca ggcaacgacc cagctcacaa tcagccagcg
    43021 cgtaacgcac cgccagattt tcgccaccgt gacggaattg tttagccgtc atgcccagcg
    43081 tttcatcggc tttgcggtag taactgctgc tgtcggggaa tccggcgtta agaatagacg
    43141 tcgtcacgct ctcccctttc gccagcgatt cgcgcaaacg gcgagcgcgc caggcctgtt
    43201 gccaggcttt aggcgtcatt ccggtagtag ctttaaacaa ccgatgtaga tgaaatggac
    43261 tcatcgccac ctggtcggct aaggcttcca gcgttacagg cgtttcctgt tccagcagtc
    43321 gacacgcgtg ggtgatttta tccaaccgat gttgccgggg attggctttg tctggctgac
    43381 aacgtttgca ggggcgaaat ccggcggcga gtgcctcgct ggcatttgcg tagaaggaga
    43441 cattttcccg caaggcatgt ctggcgcggc aagaaggacg gcaaaagata cccgtggtgc
    43501 gcacggcgaa aacgaattcg ccgtcggcat tcgggtcgcg ggctaagaca gattgccagc
    43561 gttgatcgtc agttaagcat gtggcgtttt tcataatcag ctccctggtt aaggatagcc
    43621 tttagtctgc ccggtcacca tcacgcaaaa accaacaatc ttgcgcttta atttttttcg
    43681 ctgacaagga agcttttaaa ctgtggtgac atccaggttt taaagctatc gccttctttg
    43741 gtgatcatat aaaccgccag cccttcccgg cgaacaacct ctttggcttt ctccggcccg
    43801 agtaccatca agccagtatc ccaggcatcg gcttccagcg ccgtcggagc aatcaccgtc
    43861 acggatacca gattgtgttc gatgggacgc ccggtttgcg gatcgataac atgggaaaga
    43921 cgtttgccgt ccagttcgta atagttacgg tagctgccag aggtgctaat cccatgaccg
    43981 ttgatatcca ccacagcctg aaccgcgttt tctttatcgg ttggtttttg aatcgctacc
    44041 cgccacggct ggccttcacc gttcatacca cggctgttca gcgcgccgcc caccgacacc
    44101 agatagcggg aaatcccttc ctgctccatc aagcgtgcca ggtgatccgc cgcataacct
    44161 tcaccgacgg tggagagatc gacatataaa tccggcaggt ctttttgcag atattgctga
    44221 tgcgactgat tgatgaccgt caggtgctgt aagccggttt tggctttcat cgcatcgatc
    44281 tgttcctggc tcggaatttg aaccggctgt tgttccgggc caaagcccca cagattcacc
    44341 agcggcccga cggtaatatc catcgcacca tcggtccttg ccccaatgcg cagcgaggtg
    44401 gtgacgatat cggccatcgc ttcacttacc ggccacggcg acaaactttg cgagtcgtta
    44461 aagcgcatca gcgcggaatc ttttttatag gtcgaaagca gctgatcgtc ggcgtccagc
    44521 tgggtctgaa tcttttcttt aagttcggca ctgcgtttgg catcaatgcc cgggatgctg
    44581 gcacgccaga aggtacccat ggttttgcct tcaagaacgg taacttcagt agcgtgggtt
    44641 ttggcgggtt gtggtttttg atcgcaacca acaaagaaga gcgcggcaac cagcagtgcc
    44701 acgcgggtaa agcttatttc catacgtgat tatcctcatg cgaacggtcg caagagtaca
    44761 ccaaaaaact gtgtttgtac gctgaaaaca atgaaaaaag ggcccgcagg ccctttgttc
    44821 gatatccatc gagattagaa ctggtaaacc agacccagag ctacgatgtt atcagtgttg
    44881 atgccagcgt cacgagtgaa ctggttgtcg tccagcaggt tgattttgta gtcaacgtag
    44941 gtggacatgt ttttgttgaa gtagtaggtc gcaccaacat caacatattt caggatatct
    45001 tcgtcgtcgt agttacgacc attgatgaca cccaggtttt tacctttaga ctgcaggtaa
    45061 gccagggacg gacgcagacc gaagtcgaac tggtactgag caacagcttc gaagttctgt
    45121 gctttgttcg cccaacccaa gttgcctacg cgagttgcgt tgtaggtctg ggtgtactga
    45181 gcagctaggt agatgttgtt cgcgtcgtat ttcagaccac cggtgtaggt ttcagcgcgg
    45241 tcgccattac cgatgtaacg ctcatcataa cggttcagac cgaagttctg gtcatcagta
    45301 cgtttggagc tggaaactgc agcaccgata ccgaagcctt cgtaatcata agtaatagaa
    45361 ccgccaacgc cgtcaccgtt ctgacgcaga gcttcacgac cgttgttggt catgccttca
    45421 ccttccgggc taccgttttt gccctgatac tgaacagcga agttcagacc gtcaaccaga
    45481 ccaaagaagt cggtgctacg gtaggttgcg aagccgttac cacgctgctg catgaagttg
    45541 tcagaaccgt aagtgtcacc accgaattct ggcagtacgt cggtccagga agttacgtcg
    45601 taaactacgc cgtagttacg accgtagtcg aaagaaccta catcctggaa tttcagacct
    45661 gcgaatgcca cacgggtcca ggagttgttt tcgttttcag cgctgttgcc ctggatctga
    45721 tattcccact ggccgtaacc ggtcagctgg tcagtaacct gagtttcacc tttgaagcca
    45781 agacgcatgt aggtctggtc gccatctaca gacttgtcgt cagagaaata gtgcaggccg
    45841 tctactttac cgtacagatc taatttgttg ccgtctttgt tgtaaacttc agcagcgttt
    45901 gctgcgcctg ctaccagcag agctgggacc aggagggaca gtactttaac tttcatgtta
    45961 ttaaccctct gttatatgcc tttatttgct tttttatgcc actgcatact gattaaccct
    46021 cattaatcag tcggcaagtc cattctcccc aaaaatgcag aataatccaa cacgaatatg
    46081 atactaaaac ttttaagatg tttcatttat cgctatagat gtttcaaaat gtaaatacaa
    46141 gggaactttt taagattatt gcggaatggc gaaataagca cctaacatca agcaataata
    46201 attcaaggtt aaaatcaata acttattctt aagtatttga cagcactgaa tgtcaaaaca
    46261 aaaccttcac tcgcaactag aataactccc gctatcatca ttaactttat ttattaccgt
    46321 cattcatttc tgaatgtctg tttaccccta tttcaaccgg atgcctcgca ttcggttttt
    46381 ttacccttct ttacacactt ttcattattc tgtgctacca cagaaaaact ataacgcttg
    46441 ttaactattt cacaaataat taacatcagc ataatttcca gcaatctttg tttatttgca
    46501 attaatttcg ttgggctttt tgtaggttat ttgtacagca aaatggcgct tgtacatcta
    46561 tttcccccca tacaggatga taaatatcac gggagaatag agaatcatca atcaggtaag
    46621 agtctggaat ttcacactgt accctttata ctgccctatc acttcgcgaa gttttaacag
    46681 gtcataaaca cgaatgcgtc agaaagagac aacggccacg acccgctttt cactcctacc
    46741 ggggagcatt acccgcttct ttttactgtt gatcattgtg ttactggtga cgatgggggt
    46801 aatggtacaa agtgccgtta acgcttggct gaaagataaa agttaccaga ttgtcgacat
    46861 tacccacgca atccaaaagc gcgtcgatac ctggcgttac gtgaccaggc agatctacga
    46921 caacattgcc gcgacgacct ccccctcctc cggcgaaggt ttacaagaga cgcgcctgaa
    46981 acaggatgtc tactatctgg agaaaccacg ccgcaaaacg gaagcgttaa tctttggctc
    47041 tcacgacaac tcaacgcttg agatgactca gcggatgtcc acttatctgg atacattgtg
    47101 gggtgcagaa aatgtaccgt ggtcgatgta ttacctgaat ggtcaggata acagtctggt
    47161 gctgatctca accctacccc ttaaagatct cacctccgga tttaaagaat cgaccgtcag
    47221 tgacattgtt gattcacgtc gtgcagaaat gttacaacag gccaacgccc tcgatgaacg
    47281 cgaaagtttt tctaacatgc gccgcctagc ctggcagaac ggtcattact ttaccttgcg
    47341 taccacgttc aaccagccgg gacatctggc aacggtcgtg gcttttgatc tgccgattaa
    47401 tgatttgatc ccaccgggta tgccgctgga cagtttccgc cttgagccag acgcgacggc
    47461 aacgggaaac aatgataatg agaaagaagg gacggatagc gtcagtatcc actttaacag
    47521 tacgaagatt gaaatctcct cggcactcaa ctctaccgat atgcgcctgg tctggcaggt
    47581 tccttatggc accttattgc tggatacgtt gcaaaacatt ctgctgccac tgctgctgaa
    47641 catcggtttg ctggcgctgg cgttatttgg ctataccaca ttccgccatt tctccagccg
    47701 cagtacagaa agcgtcccca gcacggcggt caataacgaa ttgcgcattt tacgggcaat
    47761 caatgaagag atagtctcac tgttgccgct cggcctgctg gttcacgatc aggaatcgaa
    47821 ccgcactgtc ataagtaaca aaattgccga tcatttgctg ccgcatctga atctgcaaaa
    47881 catcaccacc atggcggaac agcatcaggg gattattcag gcgacgatca ataacgagct
    47941 gtatgagatc cgcatgttcc gcagccaggt cgcgccgcgc acacaaattt tcattattcg
    48001 cgatcaggat cgcgaagtgc tggtaaacaa gaaactcaag caggcgcagc gtctgtatga
    48061 gaaaaaccag caggggcgga tgacctttat gaaaaacatt ggcgatgcgc tgaaagaacc
    48121 cgcacagtcc ctggcggaga gtgcggctaa actcaacgcc ccggaaagca aacaactggc
    48181 gaatcaggca gatgtgctgg tgcggctggt cgaagaaata cagttagcga acatgcttgc
    48241 ggatgatagc tggaaaagtg agacggtgct gttctccgtg caggatttaa ttgatgaagt
    48301 tgtgccttca gtgttgcctg ccatcaagcg taaaggtctg caactgctga ttaacaatca
    48361 tctgaaagca cacgatatgc gccgcggcga tcgcgatgcc ttacgacgta ttttgctgct
    48421 actgatgcaa tatgccgtga cctcaacgca attgggaaaa atcacccttg aggttgatca
    48481 ggatgagtcc tctgaagacc gcctgacatt ccgcattctg gacacgggag aaggcgtaag
    48541 tattcatgaa atggataatt tgcacttccc gtttatcaac cagacccaaa acgatcgtta
    48601 tggaaaggcg gacccgctgg cattctggct gagcgatcaa ctggcacgta aactgggcgg
    48661 tcatttaaac atcaaaacgc gggatgggct tggtacacgc tactctgtgc atatcaaaat
    48721 gctcgccgct gacccggaag ttgaagagga agaagagcgt ttactggatg atgtctgcgt
    48781 aatggtggat gttacttcgg cagaaattcg gaatattgtc actcgcctgt tagaaaattg
    48841 gggtgcaacc tgtatcacac ccgatgaaag attaattagt caagattatg atatcttttt
    48901 aacggataat ccgtctaatc ttactgcctc tggcttgctt ttaagcgatg atgagtctgg
    48961 cgtacgagaa attgggcctg gtcaattgtg cgtcaacttc aatatgagca acgctatgca
    49021 ggaagcggtc ttacaattaa ttgaagtgca actggcacag gaagaggtga cagaatcgcc
    49081 tctgggcgga gatgaaaatg cgcaactcca tgccagcggc tattatgcgc tctttgtaga
    49141 cacagtaccg gatgatgtta agaggctgta tactgaagca gcaaccagtg actttgctgc
    49201 gttagcccaa acggctcatc gtcttaaagg cgtatttgcc atgctaaatc tggtacccgg
    49261 caagcagtta tgtgaaacgc tggaacatct gattcgtgag aaggatgttc caggaataga
    49321 aaaatacatc agcgacattg acagttatgt caagagcttg ctgtagcaag gtagcctatt
    49381 acatgaacaa tatgaacgta attattgccg atgaccatcc gatagtcttg ttcggtattc
    49441 gcaaatcact tgagcaaatt gagtgggtga atgttgtcgg cgaatttgaa gactctacag
    49501 cactgatcaa caacctgccg aaactggatg cgcatgtgtt gattaccgat ctctccatgc
    49561 ctggcgataa gtacggcgat ggcattacct taatcaagta catcaagcgc catttcccaa
    49621 gcctgtcgat cattgttctg actatgaaca acaacccggc gattcttagt gcggtattgg
    49681 atctggatat cgaagggatc gtgctgaaac aaggtgcacc gaccgatctg ccgaaagctc
    49741 tcgccgcgct gcagaaaggg aagaaattta ccccggaaag cgtttctcgc ctgttggaaa
    49801 aaatcagtgc tggtggttac ggtgacaagc gtctctcgcc aaaagagagt gaagttctgc
    49861 gcctgtttgc ggaaggcttc ctagtgaccg agatcgctaa aaagctgaac cgcagtatta
    49921 aaaccatcag tagccagaag aaatctgcga tgatgaagct gggcgtcgag aacgatatcg
    49981 ccctgctgaa ttatctctct tctgtgacct taagtccggc agataaagac taatcatctg
    50041 taggccagat aagacgcgtt agtgtcttat ctggcatttg caccgattgc cggatgcggc
    50101 gtaaacgcct tatccggcct acgattccca ttatttcaac agattacatt aaagtaggcc
    50161 agataagacg cgtcagtgtc ttatctggca tttgcactga atgccggatg cggcgtaaac
    50221 gccttatccg tcctacgaat cccgcgattt cctgaccctc tcggcatata ccgtcagcgt
    50281 ctgttttatc acatccagcg ttaccggctt cgagaggcag ctgtccatac cggactccag
    50341 acaccgctgc ttctcttcag ccaacgcatt agcagttact ccgattaccg gcaacgtcag
    50401 tcccaactga cgaatgcgtt gcgtcaagcg gtaaccatcc atatttggca tgttgacgtc
    50461 gctaagcacg atatcaatat gattcttact aagtacatta agcgcatcga cgccatcatt
    50521 cgcggtttta cattgatagc ccaacgatcc caactgatct gccagcagac gtcggttaat
    50581 cggatgatca tccacgacca gaatcatcat atcgtcgtta tcgctgaccg ctttgtccgt
    50641 cgacggcaga gcgttagcag gatcgtcgct ctccatctcg atcaaataaa tacgcgccaa
    50701 caatgccggt agctcatgcg gagcagccac actgtgtacc cactcccctg gcgctttctc
    50761 cagcggaata ccaatatggc gacgacagaa ggtcactacc gctctgccct gccatttttt
    50821 actcactacc tcgtcagtga ttaacacatc ttcgggagtc ggttcctgcc cttcgtatgt
    50881 tgtaacgacg atgccgctgc gctgcaaact ggtttccagg aactgacaga gcgacgcatt
    50941 gcggaccgcc agccagcagc gtttaccact caacccttcc acgccttttt tctgcgggta
    51001 ctgagcgccg tacaacggaa tacgcacggt aaactggctg cccattcccg gttctgaatc
    51061 taccgagata tcgccgtcca tcatgctgat cagtttttca caaatcgcca gacccagacc
    51121 ggtcccctgg aagtttcgct gtacgcccgt tccaacctgg aagaagggat caaacaagcg
    51181 caccacttct ttcgccggaa tccccacgcc ggtatcgcga acacggatag agagataatc
    51241 gccatcagcg cgaacatgca aaactataca gccggtatcg gtgaatttta tggcgttact
    51301 caacaggttg gagatgacct gctgtaaacg catcgggtcg ccatttaagg ccactggcac
    51361 atccggttca ataaagcagt acaagcctaa ctgcttgcgt accaccagcg gtaaatagtt
    51421 ggcggtgatg tggttcatca cttcacgcgg tgaaaactca cgcggttcga tcttcaactg
    51481 ttccgattca atcttcgaga aatcgagaat atcgctgata attttcaaca acaggctgga
    51541 agagttgttc attaccgtca ccagacgatc gacgcctttc ggtaactctt tggtttgcaa
    51601 cagatccagg ttaccgataa tgccatacag cggcgttcgc agctcatgac tgacggtggc
    51661 aaggaacatc gattttgact ggctcgcctg ttccgctgct tgtgccatct cctgcaacga
    51721 ctcttccatc ttcacgcgcg aagaaacatc caccagcaca caaatggcca cgttttcatt
    51781 acgatagcgc gaatggacga agctgatttg cagattggta ttgttgctgg tcaggacatc
    51841 aacaaaattg acctgctgcc cacagataat ttgcgtcagt cgttggcggt cctcatgcgt
    51901 aagcatattg agataggtat gcgccagttc gttacttaaa atattgacgc catcagcggt
    51961 acgcaaaatg cagataccca ctggcgcgga ggcgacaatc ttgcgattga actgctcatg
    52021 ttcttccagt cgcagggcgt cgctttccgc cggaatgaaa atacgtcgct cgtacatccg
    52081 cgcgagagta aacaatgcag ctccggcaag cacattcagc aaaattgcgt taaggatcaa
    52141 catgcgaatg cgttccagca ccttatcaac cggcaccgaa tacacgatgc ttagcgatga
    52201 gggtggcaga tttttcttca gcaccagctc ccggaaccct tccgtatagc caaaccagga
    52261 gcgttcctgc atccagcgag gatcgccctt aattttactt tctggtccgg taagcgaaat
    52321 cagggtatga ccattttcat caagaatggt aacccccatc ggcaacgtac ccggtaagaa
    52381 aaagttctcc atccggatgg tctgctcgac acccaaaagc gcctgcaacc ggttcgccag
    52441 ataaactggc gtcaacgcgt aaaaataccc gacgcaaggg cgcggacctt cgctgatcca
    52501 gtagaggtta ctgccgctat catcttgtgg tgcatttcga tatttattga tgcgttcatg
    52561 caacgctttc aacgcggtat cgcgttccac tggcatatca cgcagaccga aattggccat
    52621 gcagaggtta tcactgccga ttaaaaatac ccggttgaga tcgtaagccg cagaaaaatt
    52681 atcgcgccag tagcgcataa accacgccaa tgactccaga gaacctcgcc aggtgttact
    52741 cattgcggaa caatcggagt cggcaaacag cggttcaaac gcaggcacat ccgcctgcgt
    52801 ttctcgtcca cgcggggaaa gcacaccgtt ttctgccgat aagcgatttt cggcgatgta
    52861 cttaagctct ttcatcacat cagaggtgcg ttgaataaag cgctgagcct gatcggaact
    52921 cagattaaat tcctgacgaa tttccgattc tcgctgatgt aacgcattaa cgatgtaaaa
    52981 aacggatgaa aaagcaatca acagccagag cactaacgcc aatgctctga acatgtagcg
    53041 cgaggctttc agggttgtac gaaaagaagc aaggtatttc aaaggggaga agctccgcct
    53101 caggtgaccg atggagtgtg gttaaggtag cggtaaaagc gtgttaccgc aatgttctgt
    53161 caccattgca ataatgcgta tcgcttagaa accattgcct taaccaggaa agccggagta
    53221 gatggacagt tctcagtacg aaaaataaga aatgaccaat ctgccgacgc ttgttaatgg
    53281 ttatatcctg gcgtcggcag atcgcccagc agtcttatct gaaaaaagag agagccaggt
    53341 tgtgtgaagg gatggatcac cagtcaaaac ttttcaccag cgtcagctcg ccagcattac
    53401 gcatcggtac aataaatgtt tcctgtttct cattgaccga tccttcatcg gtgatcagcg
    53461 tcagttgggc ggtcgtcaat tccgtttcgc tgcgcccacc atagtagttg atatacacct
    53521 gatagcgccc gtgaactggt gctggcatgg cgaaaatctc gggtccgtac cccgtcgtga
    53581 catccatatc cagtgcacca ctgtttttca gcacggtgtt accgtaccag gcgtgttcgc
    53641 catcgggcgt gacaacatgg agatcgagat cggtattgtc cgtatcccac gagagaacca
    53701 gccgtaaacg tgcacgaata gttcctgtac ccggcgttga gtaaaactgc attttttgcc
    53761 ggctttgccc atccgggctg atgacctgca cgctgttgct gccttcagtg aaaatataag
    53821 ggcgtgcaaa tgaaccatcg gattcaattc tctgcggcat actggcaccg ttgaccacca
    53881 gccggccctg ctgaacttta cccgccgcag cataattctt tattttgccg cggatctgcg
    53941 ctgagatatt ttgatcatct gccatgttga cggacgacgc cgggtaattg atcgattgtg
    54001 aaaaactggc atcttcgccc tctgccggat gccagccaga gagcggtgcg tcaatctcga
    54061 cttcctgcac accttcactg tgagcgacag gcgaaagtgc caccagtaac aacggaagaa
    54121 aaatctttcg catagcatta gtccattaat aattgtcggg tcagcgtttc gatatagttc
    54181 tcatcaagcc cggtgggata accatcaaag gccaagtgca gatactcatg ggttaaatcc
    54241 agccgatcct gaagcgtaaa gaagttacga ataaacagcc gtttctgctg ccgatcggta
    54301 taggggaaac ctgagactaa acgacagacc gcaaacacgt ctggttcgtt gtaccccgtc
    54361 tcgccttgta atatacgccg ccactgcggc atttttttcg ccagccaggc tttcgctttg
    54421 ggtaataact ggcaggttga gcgtggagct ccccagcggc taaggctgtt gtcgggataa
    54481 gcaaacgcga gtatctggtc gtaacgctcc ccctgccccg cctgggccgt ggcttgccgc
    54541 caggaaagtg tcccttcggt agcgcgactg ccatgataat gaaccggatc gcctgcatag
    54601 atgagctcct gcgtccaggc ggtcatcgtt cgcgcccccg tggtcgctgg cgaagcagaa
    54661 acacgctgcg tggcgctgct gtcggggatg gtcaggcaat caccttcccg attggcgttt
    54721 tgctgtaaaa aagtacgtat cgccacagtc atggctttcg ctgcctcagg tggcgtactt
    54781 ttcgcctcgc gatccagaac tctggtaacg tactcttcac gatcgagatg cgactgcaat
    54841 ttcagtttgc ccttttcgct cagtaaggtc gtctcaccgt ggctgacaaa cgtaatgtgg
    54901 ttaccattag taaacgtcac acgatagcgc ccattaagta cgctgggttt tactgccgtc
    54961 gtactctttt ccgctgtgat tttcttcagc ggatagcggg caaacagctc cacctctaca
    55021 cattgcccac tggcaacctg cgtcggcact ggcaaaaccc gattaagcac cgtcgcataa
    55081 cgggttagta ccgttttgct ggtgccactg ccagtaaccc acagcggtgt accatcggtt
    55141 aaccagccag caaaaccacc ctgacggatt tcctgtttat catcggcaaa ccagctccag
    55201 gttttcaccc gtacccgact accgagcatg gatgcgacac cgattttcgc ctcgtccagc
    55261 accacatcca gtaagacttc ctgagctttg ttttgcgcag gtaacgtcgc taatgaatca
    55321 agcagcgatt taaccgttac cgaggtttct ggcttcagcg ttgtaagaga cgctaaccac
    55381 gcaggtgcct gacgctgttg ccagtattgc ccccacacat ctgcgccaat atgcaaccgc
    55441 tgcggcgcaa aatagagccc acaggaacgt accagcgcgg tatcgcgcgt tatgctctcc
    55501 ccagcctgac aacaataaac ctcttccggg gagtttccgc gacactggta gggctgctcc
    55561 ggttgatggg tatcctccag ccaggcgtaa acatacagtt tccacaaact ccctaacggc
    55621 gtggtgagcg agtcaggtaa tgctgagact ttggtcacgc ctgatgacga cagctgatac
    55681 agttgatcgt gctgcgcacc gcgcagcacc agttgtaacg gcgtctcctc cgcaagcgtt
    55741 ggcagagtaa ccaacgccaa tagccagacg attctgcgcc agttcactta tttcacctgc
    55801 atcccggtcc attcactccc tgccgcgaca ctttgctgcg caggtgcata ggaacgcaca
    55861 taacgagcag gaggcagaac gaattgccct ttttgcgaga agcgcagcaa atggcggaaa
    55921 gtgaccgttc ccgtcagttc tttcaccggc accatatagg ccagttcgcc catttcatta
    55981 cgcgcttttt ccagcaattg cccctgctgt ttcgcggcgt tgggtttatt gactgaaatg
    56041 ccccatgttg tgcgctcaac gtcggctccc ggcgggagcg gtacttccac ctgaccatag
    56101 cgcagaactg catcctgctc gctggtaagc gtaatttcat cgagatacag cgcatcgctg
    56161 tcaatctcat tgctggtcac cggctgcaga gtaaagctca tctcttcttc accggggata
    56221 agccgataca actggcgttc aacggtcacc ggaatgttac tttgttgagc cgtttttgcc
    56281 ggttcacgcc agcggacctg cacatttgcg gcgataattc gtcaccaaaa gagagaatgt
    56341 ccggcacacc ctaaccaacc caacgccagt cttcgccccc tccagttaac ttatgtttag
    56401 cccatgcgcc cgcaggcgca ggcaaaacaa ctggaggcat tgtcgccata tatttcgcca
    56461 gccagttcat ggccagcgcg cgctcaatag tggattgctc agcggtcaaa ccattaaaat
    56521 agcggccgtc tgggtagcgt caccaccgct attgagcagc acaaccgtgt gcgccagcgg
    56581 ctgattgctg tttgccgctt tatcccacgc atattgctga acctgtggca tcaccgtgga
    56641 cttcaactgc gcttttttca gcaacgtcat ggttaagacg tttgccaccg catctgccag
    56701 tggagattcc ggtgtatcga ggatcaggct gtcattgata tcgcggatgt cttcctcaga
    56761 gaaatcttca tctttagttc cgcgccgggc gatagcttca tccccccctt tcaacaacgt
    56821 tttgcacggc agattcatct cctgtgccca cgccagcacc agcgcccgat gcaataacgg
    56881 catgttatcc gcctgctcgg cataactgtc gagcatatgc tgccagtatt ccggttgttg
    56941 cgttacgccg agcgcctggc tggcctgcca gtcggcgtac catgcccatg ccgtaaggaa
    57001 ggcgtcacca ttgccatctt caccccacca ggtaaagcgc gctccgggcc ccgccagttg
    57061 catcagccgc agacggttat cctgaatcat ctgacgaatg tcgttagcgg cggcactttg
    57121 atgatcggca agcgaacgcc aggcgagact gagcgggatc agacggctac cggtgttgat
    57181 caccccaccc cacggttcat cgactaacgc atcaagattg ttgcggaaaa tctcctgcgg
    57241 cgtttcacta ctttgtagcc ggatattact cgcctgctcg ggcaacatca gcgcgttctc
    57301 gccaccgccg agcatgacat ttttctgctg ttcaacgggc cagctgttgt ccacaaaaga
    57361 cagttttgtg ctaatgctgt cctgcacttg cccattttgt tgcagttctg cacttaacaa
    57421 gccagattgc tgaatattct gcgtcagcga aatataattc gcccctttgt gcagcgtcag
    57481 cgtctggcgc atctcagcgc ctgcaaattt agtcaccagc gccaccggtt cgttatcctg
    57541 ctgactgaag ataaacagtc ctgccgccgg tttgtcgccc acgcgataca ccgttggcat
    57601 actccacttc atgtagagat ttttttccga acgcagataa gcacgcccct gcccgaccag
    57661 cccgtcgccg ttcatcccac gcgcggtgat acgccagcgg gttaacgaat caggcatcag
    57721 gaacgtgaaa tacgctttgc cttgtttatc ggttgtgagt gacggcatcc atgccgcggt
    57781 atccacctct tcacgccgtg gacgttcaag catttttact cgccgctcgc tgcggttagt
    57841 cgcgccaggc gcaaccggct cgctagagag cgcctggtca tagctgataa acgacagact
    57901 ggagctggta cgcacattgt tacgccccag cggatagaaa aatttgccga tattcggcgc
    57961 gatttctggt tgcagcgcgt agatcatttc atcgaccacg cctaccgtta gctgcgcaga
    58021 aacaggttta cctttcagcg acgaggttaa t
//
LOCUS       DBKELA010000012        56903 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.012, whole genome shotgun sequence.
ACCESSION   DBKELA010000012 DBKELA010000000
VERSION     DBKELA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.012"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACUQR0_000717"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACUQR0_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HHV9107884.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACUQR0_000718"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACUQR0_000718"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HHV9107885.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACUQR0_000719"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACUQR0_000719"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HHV9107886.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACUQR0_000720"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACUQR0_000720"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HHV9107887.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACUQR0_000721"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACUQR0_000721"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HHV9107888.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACUQR0_000722"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACUQR0_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HHV9107889.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACUQR0_000723"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACUQR0_000723"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HHV9107890.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACUQR0_000724"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACUQR0_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACUQR0_000725"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACUQR0_000725"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HHV9107891.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACUQR0_000726"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACUQR0_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HHV9107892.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACUQR0_000727"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACUQR0_000727"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HHV9107893.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACUQR0_000728"
     CDS             complement(13538..13933)
                     /locus_tag="ACUQR0_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HHV9107894.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACUQR0_000729"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACUQR0_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HHV9107895.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="ACUQR0_000730"
     CDS             complement(15220..15447)
                     /locus_tag="ACUQR0_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HHV9107896.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="ACUQR0_000731"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="ACUQR0_000731"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HHV9107897.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="ACUQR0_000732"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="ACUQR0_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HHV9107898.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACUQR0_000733"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACUQR0_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HHV9107899.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="ACUQR0_000734"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="ACUQR0_000734"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HHV9107900.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="ACUQR0_000735"
     tRNA            19689..19765
                     /locus_tag="ACUQR0_000735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19799..19875
                     /locus_tag="ACUQR0_000736"
     tRNA            19799..19875
                     /locus_tag="ACUQR0_000736"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19833..19835,aa:Met,seq:cat)
     gene            complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="ACUQR0_000737"
     CDS             complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="ACUQR0_000737"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HHV9107901.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21435..22766
                     /gene="argA"
                     /locus_tag="ACUQR0_000738"
     CDS             21435..22766
                     /gene="argA"
                     /locus_tag="ACUQR0_000738"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HHV9107902.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22828..24654)
                     /gene="recD"
                     /locus_tag="ACUQR0_000739"
     CDS             complement(22828..24654)
                     /gene="recD"
                     /locus_tag="ACUQR0_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HHV9107903.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24654..28196)
                     /gene="recB"
                     /locus_tag="ACUQR0_000740"
     CDS             complement(24654..28196)
                     /gene="recB"
                     /locus_tag="ACUQR0_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HHV9107904.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="ACUQR0_000741"
     CDS             complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="ACUQR0_000741"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HHV9107905.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31253..34621)
                     /gene="recC"
                     /locus_tag="ACUQR0_000742"
     CDS             complement(31253..34621)
                     /gene="recC"
                     /locus_tag="ACUQR0_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HHV9107906.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34634..34966)
                     /locus_tag="ACUQR0_000743"
     CDS             complement(34634..34966)
                     /locus_tag="ACUQR0_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HHV9107907.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34951..35358)
                     /locus_tag="ACUQR0_000744"
     CDS             complement(34951..35358)
                     /locus_tag="ACUQR0_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HHV9107908.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35355..35918)
                     /locus_tag="ACUQR0_000745"
     CDS             complement(35355..35918)
                     /locus_tag="ACUQR0_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HHV9107909.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35909..36379)
                     /locus_tag="ACUQR0_000746"
                     /pseudo
     CDS             complement(35909..36379)
                     /locus_tag="ACUQR0_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="ACUQR0_000747"
     CDS             complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="ACUQR0_000747"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HHV9107910.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="ACUQR0_000748"
     CDS             complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="ACUQR0_000748"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HHV9107911.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="ACUQR0_000749"
     CDS             complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="ACUQR0_000749"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHV9107912.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="ACUQR0_000750"
     CDS             complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="ACUQR0_000750"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HHV9107913.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41532..41678)
                     /gene="ygdT"
                     /locus_tag="ACUQR0_000751"
     CDS             complement(41532..41678)
                     /gene="ygdT"
                     /locus_tag="ACUQR0_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HHV9107914.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            41864..42553
                     /gene="mutH"
                     /locus_tag="ACUQR0_000752"
     CDS             41864..42553
                     /gene="mutH"
                     /locus_tag="ACUQR0_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HHV9107915.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42622..43335
                     /locus_tag="ACUQR0_000753"
     CDS             42622..43335
                     /locus_tag="ACUQR0_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HHV9107916.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43473..43691
                     /gene="ygdR"
                     /locus_tag="ACUQR0_000754"
     CDS             43473..43691
                     /gene="ygdR"
                     /locus_tag="ACUQR0_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HHV9107917.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43799..44839
                     /locus_tag="ACUQR0_000755"
     CDS             43799..44839
                     /locus_tag="ACUQR0_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HHV9107918.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="ACUQR0_000756"
     CDS             complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="ACUQR0_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HHV9107919.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46055..48214)
                     /gene="aas"
                     /locus_tag="ACUQR0_000757"
     CDS             complement(46055..48214)
                     /gene="aas"
                     /locus_tag="ACUQR0_000757"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HHV9107920.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48800..49831
                     /gene="galR"
                     /locus_tag="ACUQR0_000758"
     CDS             48800..49831
                     /gene="galR"
                     /locus_tag="ACUQR0_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HHV9107921.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="ACUQR0_000759"
     CDS             complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="ACUQR0_000759"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HHV9107922.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51222..52157
                     /gene="lysR"
                     /locus_tag="ACUQR0_000760"
     CDS             51222..52157
                     /gene="lysR"
                     /locus_tag="ACUQR0_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HHV9107923.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="ACUQR0_000761"
     CDS             complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="ACUQR0_000761"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HHV9107924.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52965..54383)
                     /gene="araE"
                     /locus_tag="ACUQR0_000762"
     CDS             complement(52965..54383)
                     /gene="araE"
                     /locus_tag="ACUQR0_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HHV9107925.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="ACUQR0_000763"
     CDS             complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="ACUQR0_000763"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HHV9107926.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="ACUQR0_000764"
     CDS             complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="ACUQR0_000764"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HHV9107927.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56612..>56903)
                     /locus_tag="ACUQR0_000765"
     CDS             complement(56612..>56903)
                     /locus_tag="ACUQR0_000765"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HHV9107928.1"
                     /translation="RCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDERRVNVNG
                     GAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERDE"
BASE COUNT        13982 a        15364 c        13974 g        13583 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag tacggtgacg
    19801 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19861 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgatctccca
    19921 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt tgccccatcg
    19981 gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    20041 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    20101 ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc
    20161 ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct gtcagcaata
    20221 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20281 acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg
    20341 gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg actggtaaag
    20401 gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc
    20461 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20521 tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt ttcgcgcgtt
    20581 ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca
    20641 atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg cggcacctgc
    20701 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20761 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20821 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg tacggtttgc
    20881 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20941 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttca
    21001 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    21061 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    21121 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21181 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21241 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21301 acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa
    21361 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca aataatcatg
    21421 caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga ttccgccatt
    21481 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21541 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21601 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21661 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21721 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcaatgagtc
    21781 tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21841 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21901 ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta atggggccgg
    21961 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    22021 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    22081 atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag
    22141 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22201 aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22261 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22321 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22381 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22441 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22501 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22561 cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca
    22621 aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga tttaccccgg
    22681 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22741 aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc aggcgtaaca
    22801 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22861 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22921 cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22981 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    23041 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    23101 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    23161 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc
    23221 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgttc
    23281 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg
    23341 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23401 ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg
    23461 taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23521 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
    23581 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc
    23641 cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg taaagcccgc
    23701 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23761 acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca gtctcgacat
    23821 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23881 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
    23941 gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    24001 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    24061 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg ccacggtggt
    24121 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24181 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24241 cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga agcgcgctac
    24301 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24361 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24421 tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct cgttattttc
    24481 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24541 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24601 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24661 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24721 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24781 ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24841 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24901 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24961 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    25021 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    25081 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    25141 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25201 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25261 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25321 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25381 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25441 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25501 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25561 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25621 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25681 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25741 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25801 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcattaaga
    25861 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25921 ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt atggatcgtg
    25981 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
    26041 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    26101 gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    26161 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26221 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
    26281 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
    26341 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26401 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26461 acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg
    26521 cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26581 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26641 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26701 atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccggctgat
    26761 ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg gctgaaaagc
    26821 ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg
    26881 gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26941 atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    27001 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
    27061 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    27121 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27181 actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat cgacaatggt
    27241 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggcc
    27301 ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa
    27361 ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc ctgattgcta
    27421 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27481 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27541 gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc ttgcagataa
    27601 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct
    27661 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27721 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
    27781 aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc atccatctgc
    27841 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27901 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27961 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    28021 agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    28081 cgcaaataga gtgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    28141 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28201 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28261 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc
    28321 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28381 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28441 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28501 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28561 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28621 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28681 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28741 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28801 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28861 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28921 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28981 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    29041 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    29101 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    29161 ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29221 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca taaggccgtt
    29281 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag
    29341 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29401 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    29461 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcgta
    29521 tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa gctctggcaa
    29581 agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt
    29641 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    29701 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt
    29761 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat
    29821 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29881 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29941 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaagccttt
    30001 atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    30061 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg
    30121 gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga acttcgctga
    30181 gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg
    30241 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30301 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30361 tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct cgtggaaatc
    30421 tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc
    30481 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30541 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc
    30601 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    30661 gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30721 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30781 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30841 atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg cctgcggatc
    30901 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30961 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    31021 tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    31081 cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc aaatcatctg
    31141 ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga
    31201 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    31261 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31321 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    31381 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31441 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31501 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    31561 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca ccgtctttgc
    31621 gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31681 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31741 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31801 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31861 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31921 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31981 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    32041 cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    32101 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    32161 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32221 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32281 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32341 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32401 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32461 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32521 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32581 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32641 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgtgcca
    32701 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32761 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32821 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32881 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32941 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    33001 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    33061 gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    33121 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33181 ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag
    33241 gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc gcacgacggt
    33301 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact gcctgaataa
    33361 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33421 gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc
    33481 tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33541 tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt tccagaatgt
    33601 cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    33661 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc ccccatgagg
    33721 ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt ccggcatttt
    33781 cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgagtca
    33841 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33901 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33961 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    34021 tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc cagcgcggtt
    34081 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc caggcctgtg
    34141 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat
    34201 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34261 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccagcaatt
    34321 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34381 ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34441 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34501 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34561 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34621 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34681 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34741 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34801 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat gttcgctgga
    34861 tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc gcaaccatta
    34921 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    34981 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    35041 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    35101 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    35161 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    35221 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    35281 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    35341 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    35401 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    35461 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    35521 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    35581 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    35641 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    35701 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    35761 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    35821 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    35881 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    35941 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    36001 agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc gaaggtgtca
    36061 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    36121 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    36181 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    36241 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    36301 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    36361 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    36421 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    36481 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    36541 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    36601 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    36661 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    36721 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    36781 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    36841 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    36901 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    36961 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    37021 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    37081 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    37141 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    37201 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    37261 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    37321 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    37381 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    37441 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    37501 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    37561 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    37621 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    37681 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    37741 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    37801 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    37861 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    37921 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    37981 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    38041 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    38101 gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    38161 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    38221 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    38281 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    38341 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    38401 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    38461 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    38521 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    38581 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    38641 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    38701 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    38761 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    38821 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    38881 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    38941 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    39001 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    39061 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    39121 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    39181 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    39241 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    39301 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    39361 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    39421 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    39481 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    39541 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct cgcacgacaa
    39601 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    39661 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    39721 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    39781 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    39841 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    39901 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    39961 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    40021 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    40081 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    40141 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    40201 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    40261 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    40321 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    40381 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    40441 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    40501 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    40561 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    40621 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    40681 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    40741 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    40801 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    40861 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    40921 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    40981 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    41041 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    41101 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    41161 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    41221 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    41281 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    41341 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    41401 cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca atgcattaca
    41461 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    41521 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    41581 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    41641 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    41701 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    41761 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    41821 cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    41881 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    41941 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    42001 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    42061 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    42121 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    42181 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    42241 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    42301 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    42361 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    42421 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    42481 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    42541 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    42601 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    42661 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    42721 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    42781 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    42841 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    42901 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    42961 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    43021 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    43081 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    43141 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    43201 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    43261 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    43321 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    43381 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    43441 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    43501 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    43561 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    43621 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    43681 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    43741 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    43801 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    43861 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    43921 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    43981 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    44041 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    44101 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    44161 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    44221 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    44281 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    44341 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    44401 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    44461 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    44521 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    44581 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    44641 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct gtcaaccgtt
    44701 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    44761 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    44821 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    44881 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    44941 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    45001 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    45061 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    45121 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    45181 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    45241 cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt tgagataggt
    45301 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    45361 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    45421 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    45481 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    45541 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    45601 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    45661 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    45721 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    45781 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    45841 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    45901 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca gagtggcaaa
    45961 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    46021 cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt tccgcttcgt
    46081 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    46141 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    46201 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    46261 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    46321 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    46381 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    46441 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    46501 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    46561 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    46621 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    46681 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    46741 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    46801 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    46861 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    46921 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    46981 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    47041 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    47101 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    47161 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    47221 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    47281 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    47341 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    47401 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    47461 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    47521 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    47581 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    47641 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    47701 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    47761 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    47821 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    47881 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    47941 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    48001 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    48061 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    48121 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    48181 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    48241 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    48301 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    48361 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    48421 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    48481 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    48541 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    48601 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    48661 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    48721 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    48781 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    48841 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    48901 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    48961 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    49021 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    49081 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    49141 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    49201 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    49261 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    49321 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    49381 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    49441 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    49501 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    49561 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    49621 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    49681 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    49741 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    49801 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    49861 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    49921 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    49981 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    50041 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    50101 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    50161 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    50221 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    50281 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    50341 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    50401 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    50461 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    50521 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    50581 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    50641 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    50701 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    50761 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    50821 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    50881 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    50941 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    51001 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    51061 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    51121 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    51181 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    51241 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    51301 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    51361 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    51421 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    51481 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    51541 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    51601 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    51661 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    51721 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    51781 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    51841 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    51901 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    51961 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    52021 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    52081 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    52141 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    52201 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    52261 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    52321 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    52381 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    52441 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    52501 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    52561 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    52621 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    52681 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    52741 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    52801 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    52861 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    52921 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    52981 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    53041 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    53101 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    53161 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    53221 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    53281 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    53341 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    53401 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    53461 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    53521 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    53581 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    53641 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    53701 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    53761 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    53821 gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact gaacgctgta
    53881 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    53941 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    54001 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    54061 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    54121 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    54181 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    54241 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    54301 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    54361 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    54421 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    54481 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    54541 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    54601 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    54661 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    54721 ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    54781 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    54841 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    54901 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    54961 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    55021 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    55081 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    55141 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    55201 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    55261 gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc tcgatggttt
    55321 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    55381 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    55441 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    55501 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    55561 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    55621 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    55681 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    55741 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    55801 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    55861 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    55921 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    55981 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    56041 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    56101 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    56161 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    56221 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    56281 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    56341 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    56401 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    56461 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    56521 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    56581 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    56641 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    56701 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    56761 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    56821 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    56881 agcctacacg ctccaggcaa cgg
//
LOCUS       DBKELA010000013        56437 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.013, whole genome shotgun sequence.
ACCESSION   DBKELA010000013 DBKELA010000000
VERSION     DBKELA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.013"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..539
                     /locus_tag="ACUQR0_000766"
     CDS             <1..539
                     /locus_tag="ACUQR0_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HHV9107929.1"
                     /translation="QPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKK
                     VQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQY
                     PARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPG
                     SGIVVNILFKINGTTEIQ"
     gene            complement(579..977)
                     /gene="yciA"
                     /locus_tag="ACUQR0_000767"
     CDS             complement(579..977)
                     /gene="yciA"
                     /locus_tag="ACUQR0_000767"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HHV9107930.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1082..1621)
                     /locus_tag="ACUQR0_000768"
     CDS             complement(1082..1621)
                     /locus_tag="ACUQR0_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HHV9107931.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(1651..2394)
                     /locus_tag="ACUQR0_000769"
     CDS             complement(1651..2394)
                     /locus_tag="ACUQR0_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HHV9107932.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(2422..2511)
                     /locus_tag="ACUQR0_000770"
                     /pseudo
     CDS             complement(2422..2511)
                     /locus_tag="ACUQR0_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2751..3389
                     /gene="ompW"
                     /locus_tag="ACUQR0_000771"
     CDS             2751..3389
                     /gene="ompW"
                     /locus_tag="ACUQR0_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HHV9107933.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(3449..3955)
                     /locus_tag="ACUQR0_000772"
     CDS             complement(3449..3955)
                     /locus_tag="ACUQR0_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HHV9107934.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4001..4501)
                     /locus_tag="ACUQR0_000773"
     CDS             complement(4001..4501)
                     /locus_tag="ACUQR0_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HHV9107935.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(4587..4766)
                     /locus_tag="ACUQR0_000774"
     CDS             complement(4587..4766)
                     /locus_tag="ACUQR0_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HHV9107936.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5147..5953)
                     /gene="trpA"
                     /locus_tag="ACUQR0_000775"
     CDS             complement(5147..5953)
                     /gene="trpA"
                     /locus_tag="ACUQR0_000775"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HHV9107937.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(5953..7146)
                     /gene="trpB"
                     /locus_tag="ACUQR0_000776"
     CDS             complement(5953..7146)
                     /gene="trpB"
                     /locus_tag="ACUQR0_000776"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HHV9107938.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7158..8519)
                     /gene="trpCF"
                     /locus_tag="ACUQR0_000777"
     CDS             complement(7158..8519)
                     /gene="trpCF"
                     /locus_tag="ACUQR0_000777"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HHV9107939.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(8520..10115)
                     /gene="trpD"
                     /locus_tag="ACUQR0_000778"
     CDS             complement(8520..10115)
                     /gene="trpD"
                     /locus_tag="ACUQR0_000778"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HHV9107940.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10115..11677)
                     /gene="trpE"
                     /locus_tag="ACUQR0_000779"
     CDS             complement(10115..11677)
                     /gene="trpE"
                     /locus_tag="ACUQR0_000779"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HHV9107941.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(11699..11792)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(11769..11813)
                     /gene="trpL"
                     /locus_tag="ACUQR0_000780"
     CDS             complement(11769..11813)
                     /gene="trpL"
                     /locus_tag="ACUQR0_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HHV9107942.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            11951..12832
                     /gene="rnm"
                     /locus_tag="ACUQR0_000781"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             11951..12832
                     /gene="rnm"
                     /locus_tag="ACUQR0_000781"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0035312 - 5'-3' DNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase RNM"
                     /protein_id="HHV9107943.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            12829..13449
                     /locus_tag="ACUQR0_000782"
     CDS             12829..13449
                     /locus_tag="ACUQR0_000782"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HHV9107944.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            13550..14422
                     /gene="rluB"
                     /locus_tag="ACUQR0_000783"
     CDS             13550..14422
                     /gene="rluB"
                     /locus_tag="ACUQR0_000783"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HHV9107945.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(14462..15052)
                     /gene="cobO"
                     /locus_tag="ACUQR0_000784"
     CDS             complement(14462..15052)
                     /gene="cobO"
                     /locus_tag="ACUQR0_000784"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HHV9107946.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15049..15807)
                     /locus_tag="ACUQR0_000785"
     CDS             complement(15049..15807)
                     /locus_tag="ACUQR0_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HHV9107947.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16027..17076
                     /gene="sohB"
                     /locus_tag="ACUQR0_000786"
     CDS             16027..17076
                     /gene="sohB"
                     /locus_tag="ACUQR0_000786"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HHV9107948.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17112..17363)
                     /locus_tag="ACUQR0_000787"
     CDS             complement(17112..17363)
                     /locus_tag="ACUQR0_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HHV9107949.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            17743..20340
                     /gene="topA"
                     /locus_tag="ACUQR0_000788"
     CDS             17743..20340
                     /gene="topA"
                     /locus_tag="ACUQR0_000788"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HHV9107950.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            20550..21524
                     /gene="cysB"
                     /locus_tag="ACUQR0_000789"
     CDS             20550..21524
                     /gene="cysB"
                     /locus_tag="ACUQR0_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HHV9107951.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            21843..21983
                     /locus_tag="ACUQR0_000790"
     CDS             21843..21983
                     /locus_tag="ACUQR0_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HHV9107952.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            21986..22153
                     /gene="yciX"
                     /locus_tag="ACUQR0_000791"
     CDS             21986..22153
                     /gene="yciX"
                     /locus_tag="ACUQR0_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HHV9107953.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22267..22362
                     /gene="ymiC"
                     /locus_tag="ACUQR0_000792"
     CDS             22267..22362
                     /gene="ymiC"
                     /locus_tag="ACUQR0_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HHV9107954.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            22526..25201
                     /gene="acnA"
                     /locus_tag="ACUQR0_000793"
     CDS             22526..25201
                     /gene="acnA"
                     /locus_tag="ACUQR0_000793"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HHV9107955.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25265..25855)
                     /gene="ribA"
                     /locus_tag="ACUQR0_000794"
     CDS             complement(25265..25855)
                     /gene="ribA"
                     /locus_tag="ACUQR0_000794"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HHV9107956.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26025..26789
                     /gene="pgpB"
                     /locus_tag="ACUQR0_000795"
     CDS             26025..26789
                     /gene="pgpB"
                     /locus_tag="ACUQR0_000795"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HHV9107957.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            26938..27246
                     /gene="lapA"
                     /locus_tag="ACUQR0_000796"
     CDS             26938..27246
                     /gene="lapA"
                     /locus_tag="ACUQR0_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HHV9107958.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27253..28422
                     /gene="lapB"
                     /locus_tag="ACUQR0_000797"
     CDS             27253..28422
                     /gene="lapB"
                     /locus_tag="ACUQR0_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HHV9107959.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            28615..29352
                     /gene="pyrF"
                     /locus_tag="ACUQR0_000798"
     CDS             28615..29352
                     /gene="pyrF"
                     /locus_tag="ACUQR0_000798"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HHV9107960.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            29352..29678
                     /gene="yciH"
                     /locus_tag="ACUQR0_000799"
     CDS             29352..29678
                     /gene="yciH"
                     /locus_tag="ACUQR0_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HHV9107961.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(29804..30022)
                     /gene="osmB"
                     /locus_tag="ACUQR0_000800"
     CDS             complement(29804..30022)
                     /gene="osmB"
                     /locus_tag="ACUQR0_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HHV9107962.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30291..31040)
                     /locus_tag="ACUQR0_000801"
     CDS             complement(30291..31040)
                     /locus_tag="ACUQR0_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HHV9107963.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(31130..31303)
                     /gene="yciZ"
                     /locus_tag="ACUQR0_000802"
     CDS             complement(31130..31303)
                     /gene="yciZ"
                     /locus_tag="ACUQR0_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HHV9107964.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            31341..31469
                     /gene="ymiD"
                     /locus_tag="ACUQR0_000803"
     CDS             31341..31469
                     /gene="ymiD"
                     /locus_tag="ACUQR0_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HHV9107965.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(31451..33425)
                     /gene="pdeR"
                     /locus_tag="ACUQR0_000804"
                     /pseudo
     CDS             complement(31451..33425)
                     /gene="pdeR"
                     /locus_tag="ACUQR0_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            33479..33581
                     /locus_tag="ACUQR0_000805"
                     /pseudo
     CDS             33479..33581
                     /locus_tag="ACUQR0_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33659..35593)
                     /gene="rnb"
                     /locus_tag="ACUQR0_000806"
     CDS             complement(33659..35593)
                     /gene="rnb"
                     /locus_tag="ACUQR0_000806"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HHV9107966.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(35661..36788)
                     /locus_tag="ACUQR0_000807"
     CDS             complement(35661..36788)
                     /locus_tag="ACUQR0_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HHV9107967.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(36932..37720)
                     /gene="fabI"
                     /locus_tag="ACUQR0_000808"
     CDS             complement(36932..37720)
                     /gene="fabI"
                     /locus_tag="ACUQR0_000808"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HHV9107968.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(38088..38441)
                     /locus_tag="ACUQR0_000809"
     CDS             complement(38088..38441)
                     /locus_tag="ACUQR0_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HHV9107969.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(38509..39315)
                     /gene="sapF"
                     /locus_tag="ACUQR0_000810"
     CDS             complement(38509..39315)
                     /gene="sapF"
                     /locus_tag="ACUQR0_000810"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HHV9107970.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(39317..40309)
                     /gene="sapD"
                     /locus_tag="ACUQR0_000811"
     CDS             complement(39317..40309)
                     /gene="sapD"
                     /locus_tag="ACUQR0_000811"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HHV9107971.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            complement(40309..41199)
                     /gene="sapC"
                     /locus_tag="ACUQR0_000812"
     CDS             complement(40309..41199)
                     /gene="sapC"
                     /locus_tag="ACUQR0_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HHV9107972.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(41186..42151)
                     /gene="sapB"
                     /locus_tag="ACUQR0_000813"
     CDS             complement(41186..42151)
                     /gene="sapB"
                     /locus_tag="ACUQR0_000813"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HHV9107973.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(42148..43791)
                     /gene="sapA"
                     /locus_tag="ACUQR0_000814"
     CDS             complement(42148..43791)
                     /gene="sapA"
                     /locus_tag="ACUQR0_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HHV9107974.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(44104..44349)
                     /locus_tag="ACUQR0_000815"
     CDS             complement(44104..44349)
                     /locus_tag="ACUQR0_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HHV9107975.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(44483..45868)
                     /gene="puuP"
                     /locus_tag="ACUQR0_000816"
     CDS             complement(44483..45868)
                     /gene="puuP"
                     /locus_tag="ACUQR0_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HHV9107976.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(45858..46022)
                     /locus_tag="ACUQR0_000817"
     CDS             complement(45858..46022)
                     /locus_tag="ACUQR0_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9107977.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(46171..47589)
                     /gene="puuA"
                     /locus_tag="ACUQR0_000818"
     CDS             complement(46171..47589)
                     /gene="puuA"
                     /locus_tag="ACUQR0_000818"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HHV9107978.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            47801..48565
                     /gene="puuD"
                     /locus_tag="ACUQR0_000819"
     CDS             47801..48565
                     /gene="puuD"
                     /locus_tag="ACUQR0_000819"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HHV9107979.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            48592..49149
                     /gene="puuR"
                     /locus_tag="ACUQR0_000820"
     CDS             48592..49149
                     /gene="puuR"
                     /locus_tag="ACUQR0_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HHV9107980.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            49299..50786
                     /gene="puuC"
                     /locus_tag="ACUQR0_000821"
     CDS             49299..50786
                     /gene="puuC"
                     /locus_tag="ACUQR0_000821"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HHV9107981.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            50788..52068
                     /gene="puuB"
                     /locus_tag="ACUQR0_000822"
     CDS             50788..52068
                     /gene="puuB"
                     /locus_tag="ACUQR0_000822"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HHV9107982.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            52106..53371
                     /gene="puuE"
                     /locus_tag="ACUQR0_000823"
     CDS             52106..53371
                     /gene="puuE"
                     /locus_tag="ACUQR0_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HHV9107983.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(53491..54468)
                     /gene="pspF"
                     /locus_tag="ACUQR0_000824"
     CDS             complement(53491..54468)
                     /gene="pspF"
                     /locus_tag="ACUQR0_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HHV9107984.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            54635..55303
                     /gene="pspA"
                     /locus_tag="ACUQR0_000825"
     CDS             54635..55303
                     /gene="pspA"
                     /locus_tag="ACUQR0_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HHV9107985.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            55357..55581
                     /gene="pspB"
                     /locus_tag="ACUQR0_000826"
     CDS             55357..55581
                     /gene="pspB"
                     /locus_tag="ACUQR0_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HHV9107986.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            55581..55940
                     /gene="pspC"
                     /locus_tag="ACUQR0_000827"
     CDS             55581..55940
                     /gene="pspC"
                     /locus_tag="ACUQR0_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HHV9107987.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            55949..56170
                     /gene="pspD"
                     /locus_tag="ACUQR0_000828"
     CDS             55949..56170
                     /gene="pspD"
                     /locus_tag="ACUQR0_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HHV9107988.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            56213..>56437
                     /gene="pspE"
                     /locus_tag="ACUQR0_000829"
     CDS             56213..>56437
                     /gene="pspE"
                     /locus_tag="ACUQR0_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HHV9107989.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVK"
BASE COUNT        14154 a        14153 c        14660 g        13470 t
ORIGIN      
        1 ttcagccgcc accggagccg gtggtagagc cagaaccgga acctgagccg atccccgaac
       61 cgccaaaaga agcaccggtg gtcattgaaa agccgaagcc gaaaccgaag ccaaaaccga
      121 agccggtgaa aaaggtacag gagcagccaa aacgcgatgt caaacccgta gagtcgcgtc
      181 cggcatcacc gtttgaaaat acggcacccg cgcgccccac atcaagtacg gcaacggctg
      241 caaccagcaa gccggttacc agtgtggctt caggaccacg cgcattaagc cgtaatcagc
      301 cgcagtatcc ggcacgagca caggcattgc gcattgaagg gcaggttaaa gttaaatttg
      361 acgtcacgcc ggatggtcgc gtggataacg tacaaatcct ctcagccaag cctgcgaaca
      421 tgtttgagcg tgaggtgaaa aatgcgatgc gcagatggcg ttatgagccg ggtaagccag
      481 gcagtgggat tgtggtgaat atcctgttta aaattaacgg caccaccgaa attcagtaag
      541 cagaaagtca aaagcctccg accggaggct tttgactatt actcaacagg taaggcgcga
      601 ggttttcctt caggatcaac cgctacatac ttaaataatg cttctgtcgc tttatagcgt
      661 tgcccgattg gttcagacgc tacttttttc acccatactt caatattaat gctgaccgat
      721 gtcgtccctt tctggacaca gcgtgcatag cagcacacca catcgccgac cgcaaccggc
      781 cgtaagaaag tcattccttc aacccgcaca gtcactacgc gaccgtgggc aatttctttt
      841 gccagaatag cgccgccaat atccatttgt gacattaacc aaccaccaaa gatgtcacca
      901 ttggcattgg tatcggcggg catggctaaa gtacgtaaaa caagatcgcc ctgagggacg
      961 ttatgtgttg tagacatggt aaaaccgact taaaaggaaa atcacaggcg cgatgctact
     1021 atgattttcc tgaggagaac agaggagata cagcgccagc cccgaaggac tggcagtctg
     1081 gttaggattt atcttcctgc ggcatgtggc ggtagatata gataccgctt aacaatgtga
     1141 agattaaggt aagggcggtc aggccaaaga ctttaaagtt gacccaaata ttttgcggca
     1201 gccagaatgc gatgtagatg tttgccagac cgcaaaggat aaagaaaaca gcccaggcca
     1261 gatttagctt cgaccatacc ggttgcggca gcgtgagttc tttactcagc atccgctgaa
     1321 ttagcggctt tttcatcacc cattggctga ctaacagggc tcccgcaaac agagcataaa
     1381 tgactgtcac cttccattta ataaactcat cattgtggaa gaacagcgtc aagccaccga
     1441 agacgaccac cagaacaaaa gtgatcaggg ccatcttctc aaccttacga aagcgaaccc
     1501 agctatagat aagcacaatc gccgtggcga cgatcagcgc tgcagtagcc gcatagatgt
     1561 cataaatctt gtaaaacgcg aaaaagacaa ccagcggtaa aaaatcaaga aactgcttca
     1621 ttttacgatt ccgtaatcaa atgcttaagg ttattggcga atcaacatat acaggcggaa
     1681 cagatagatg agcaatatgg cagaaatcag gttgctcaag gtgttcgcca gtacggcacc
     1741 aatttccggg gttaatgcgg caaaagaaga ggcaaaaagc agcaacagtg tttttgccag
     1801 caaccagctc agtactgcgg gtgccaccag acgcatattc gcccaagtca gccgcatact
     1861 gctacgcatc gaggcaaaaa tgcccatttt gtcctgtacc agcatcaccg gtgccagggc
     1921 cagtaaaatg gccatgataa ttcccggaac gaccaccagc atgatgccaa tctgtactaa
     1981 aagggtagtc agaaaaatca gaataaataa cttgggcaat atcggcgcac tggcaccaat
     2041 agctcgcagc gcactgactc tctgacccgc agacaccagc tggataatta atattacgcc
     2101 tccggcgaga atggcgttac cgattaattc tgaaaaagtg gacgccgctg aagcctgcag
     2161 caaaatttgt tgctgttccg gtgacatatt ctgaaccagg tcgaacaacc cactactgcc
     2221 gctaacgggc acgccgtcat tgagctgcgc aagctgtgca tcactgggtg agaaaacatg
     2281 ccctaacacc actgtgataa acgcacatag caacgatacc aacagaatgg tcataaattg
     2341 attacggaag aaatttccgg tgtcacggta tacggactgc gccgtgatag acatgcactc
     2401 tccttgagtt ttatgcaggt gttaattagc gggcaattgt accctggtta agcctcgggt
     2461 ggcagcatca ggtcttgtat ggaaaagcat atctttgtaa agcggaggta attgctggcc
     2521 ttcaggcgtg ggcaaaccgc ggcaagtggc gatcgagtta atatttgcgt agagaaaata
     2581 tttaaaaatt gatttaaatc acattaacca ggattctcaa tgcaacttct aaattaatcc
     2641 agatcaataa atggtgaatt atcatatgta atgtgatcta tgtaggatca tttgttactc
     2701 caatgtaggt atattcgtca cgtttttata accataacga cggagcggat atgaaaaagt
     2761 taacagtggc ggctttggca gtaacaactc ttctttctgg cagtgccttt gcgcatgaag
     2821 caggcgaatt ttttatgcgt gcaggttctg caaccgtacg tccaacagaa ggtgctggtg
     2881 gtacgttagg aagtctgggt ggattcagcg tgaccaataa cactcaactg ggcttgacgt
     2941 ttacttatat ggcgaccgac aacattggtg tggaattact ggcagcgacg ccgttccgcc
     3001 ataaaatcgg cacccgggcg accggcgata ttgcaaccgt tcaccatctg ccgccaacac
     3061 tgatggcgca gtggtatttt ggtgatgcca gcagcaaatt ccgtccttac gttggggccg
     3121 gtattaacta caccaccttc tttgataatg gatttaacga tcatggcaaa gaggcggggc
     3181 tttccgatct cagtctgaaa gattcctggg gagctgccgg gcaggtgggg gttgattatc
     3241 tgattaaccg tgactggttg gttaacatgt cagtgtggta catggatatc gataccaccg
     3301 ccaattataa gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt
     3361 ttatgttctc agcaggatat cgtttttaat tccgcacaaa aacgaccccg taatatacgg
     3421 ggtcaataag gacatggtat aaagggtatt atttcttcgc ttctacgcca tcagtttcag
     3481 aacgaattaa aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt
     3541 gcttttcctc atttaaaatc gcttcgatga tcggaattga gactgtatca ccggcatttt
     3601 ttgctgctgc taatagtgag gtgtaacagg tgatttcaaa ttgctcgaag acatatccgc
     3661 caatagagcc tttaactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg
     3721 cagccatttt actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa
     3781 tagtttccag ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc
     3841 gtagttcagg ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct
     3901 tttccattgc gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa
     3961 tgtcctcttt ataaaatgac taaaagttaa aattcatatt tcaggcttta ttttcggctt
     4021 tcttatttac gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt
     4081 ccagggtttc tttcagaagc ttcgctgctt tacggtaacc taattgttca gccagcgtcg
     4141 ctaatgtccc gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg
     4201 cggcatcacg cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa
     4261 gaccttccat tgccacacat ttcatgcgct taattttcag atttgattct gattccacaa
     4321 cttgatcaat tcgttcaatc tgtccatgag tttcctcgag gtgcgcatga aaagcctgac
     4381 ttaatttttc atttgatgtt gctcttgcga gttttgccag tgcccgggtt aattgttttt
     4441 ctgcgctgta ggtatctgaa agcagttgaa taaatacatc ttcaatggtc ttcatattca
     4501 tatttttctc cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc
     4561 agctgatatt aataattaac ggtgaatcag gatttgcctg atttattacc accgctttgt
     4621 tgaccgcctt ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg
     4681 ctatgctgac cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt
     4741 cctgaactac cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta
     4801 atatgagtgt tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt
     4861 aagcgcaacg agaagataga gggaaaatat attttgagga acattctgga tatattaaca
     4921 attacctgag gaatatgtga cttagagaaa attattgatt ttactggtgt tatgttgcgg
     4981 gattaatttg tttgattata ttactgttgg gcggaaaaat gacgtaagtt gacgttcgac
     5041 cggggtaagc gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc
     5101 attaaagaaa gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg
     5161 ccgctttcat cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa
     5221 tatgttgctc gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat
     5281 caatcgctgc ttttacctga tccggggcgg aaataccaaa cccctgcaat ggaggtgcag
     5341 cgttgtactc tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg
     5401 cgccggtcac gcccgctcgt gacagcaaat atgtgtaacc acgaccgtaa gaggctatct
     5461 ggcgcagcag gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatggc
     5521 gcaacgcggc ctggcggaag ggcgcggact cttcaactgg cacatcggca accagcaccg
     5581 aatcgacgcc gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca
     5641 gattggcata catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca
     5701 gcatttcaaa acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt
     5761 gaatggtcgg gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag
     5821 caccggcttc aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga
     5881 gcgtgacgaa aggaacgaat gcgccttctt tgcgctcctt caactgggca aacagagatt
     5941 cgtagcgttc catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc
     6001 tttatcgccg cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg
     6061 catcattttc agcgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc
     6121 gtgcaggcac agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc
     6181 agcgcgtcca gtgctgttaa gatacgcgtg ttgtggaccg acagacggga aatccagtcc
     6241 ggcggagatg gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt
     6301 cataccgaaa taaatgccca cgcgaccatg ttttagcggt gcgccgtgct cgccagtttc
     6361 gataccgtga ccaccaggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc
     6421 agcaaacatg ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag
     6481 gcgaccttct ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc
     6541 acggacaatg gtcggataag gatgcgggcc agccgcggtg cccagcatat agtgcgcggt
     6601 ttcataactg ccggaccagt cgcgtagcgc ctcattacag gcatctttca gcgtggcgga
     6661 accgctatgt accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga
     6721 ctggcgttca acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct
     6781 ggcaagggcc gacgccacgc catgctgacc ggccccggtt tcggcgatga tttcggtttt
     6841 acccatccgc ttcgccagca acgcctgccc cagcacctgg ttggttttat gtgcgccgcc
     6901 gtgcagcaaa tcttcacgct tgagatacag cgtggtgttc gtcccggctg taatgttctg
     6961 gcatttggtc agtgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgggc
     7021 ttgaaattca ggatcttttt gcgcgctgac aaaagcttct tccagctggc gcagagcagg
     7081 catcaggatt tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt
     7141 tgtcattgtt cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt
     7201 gcgtctttga tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg
     7261 gtttgtgccg cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca
     7321 agcgattgac catttaatag tgaccagtca aaacgttgcc cgcttccacc ctggccgttg
     7381 tctaaaacat atttatcaac gtgctgaaac tcgcgggcgg gcagggtttc accgacgctt
     7441 aatgctttcc agatggcaac atgtgctggc agagcttcac gcagcgtatc gatatacagc
     7501 tgatcttcat taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc
     7561 acatcggcaa tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc
     7621 acttcctgcg cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca
     7681 ccgtaaatcg cgcccgcgtc ataagctgct ttagcatctt gcccacgcgt caggccacat
     7741 actttattct cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc
     7801 aacgccgaac caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa
     7861 gtattgatgc cggattcgct gattaccgtt acgttgtgcc ccagtttcgg cgcaagctcg
     7921 cgggtacggt tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc
     7981 tttgccccta atgcaatggc gcgctccagt tcctcttcat tactgacttc ggtcagcacc
     8041 cccatctcca gactgtgagc gacggctgca agctggcgat attggtcgtc atccagtact
     8101 gaaagcatta ataagcaggc atcggcctgg taatagcgcg ccagatagat ctggtaaggg
     8161 tcgataatga agtctttaca taaaatcggc tgcggggcga tttggctgac gatgaggagg
     8221 aaatcaaagc tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa
     8281 tgtttataaa tggcggcaat gcgtgccgga tcgaaatcat cacggatcac gccttttgac
     8341 ggcgacgctt ttttacactc cagaataaac gccgtgcgtg caccctgtag cgcatcataa
     8401 aaatgtcgtg tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg
     8461 cgggtttcta cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt
     8521 taccctcgtg ccgccagcgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca
     8581 agaacggttt gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg
     8641 acgttcgcag cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt
     8701 aaaatgtcac ggttttcttc aggtgttccg cctgccagtt gctcctggtg gtagggagtc
     8761 aggccaaaat cttcagcggt caattgatag ctcttaattt tgccgtcatg cagctcggca
     8821 acgattgtcg gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg
     8881 cgttgatacc ccagcacgcg caatgtttcg gcaatcggca gcaccagttc cggactataa
     8941 acaccaatta acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac
     9001 agggtgcggg ttttcagttg ctggcgaacc ggcatcgcgt ggcggaatcc ggtgtgatat
     9061 ttcggcgcaa agaggaaaca tacgcctaac tcgtccagcg cctggcgcga tttattggcg
     9121 ttcatatcaa gattaatacc gaatgccgcc agcagatccg acgagccgga tttactggag
     9181 acgctgcggt taccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg
     9241 gcggtagaaa tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac
     9301 agataatccg ggcgcgggaa cggcgctgcg ttttccagta gcgcggttgc tgccccggcg
     9361 atctcgttcg ggtgctcacc gcgaattttc atgctcacca gcgccgccgc cagttgttcc
     9421 ggcttcagct cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc
     9481 gtctgcgcct gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc
     9541 tgctgcgccc aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat
     9601 tccggatgga actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg
     9661 ccattaaaat gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga
     9721 taacgcgcca ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca
     9781 atgctcgacg ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct
     9841 tcgacaattg cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg
     9901 gtgaggagtt ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc
     9961 agtaccggat tgctcatcgt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg
    10021 cggtaaatca ccacgttatg accattgctg cgcaactgat ctgccaggtt gtacgtaaaa
    10081 gagtcgatat tatcgagcag cagaatgtca gccatcagaa agtctcctgt gcatgatgcg
    10141 cggtggcaat agcgcgcagt acagcgcggg ctttgttacg ggtttcgtcg gcttccgact
    10201 gcggaacaga atcaaggact acaccagcac ccgcttgcac ggtggcgata ccgttttcca
    10261 ccagcgccga gcggatcaca atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta
    10321 ccgcgccgcc gtagctgccg cggcgacgac cttccgcctc ggcaattaac tgcatagcgc
    10381 gtactttcgg cgcaccgctt aacgtcccca tattcataca ggcgcgataa gcgtgcaggg
    10441 cgtcaagatc gtgacgcagt tcgccgacta cgcgagagac gaggtgcatc acataggaat
    10501 aacggtcaac tttggtgaga tcggcgatgt agcggctgcc aggggtgcaa atgcgtgcca
    10561 gatcattacg ggcgagatca accagcatca gatgttcaga cagctcttta tggtcagtac
    10621 gcatttccag ttcaatacgg ctgtcgagat ctctgtccag tgaaccatcg gcgcgacgac
    10681 cgcgtgggcg tgttccggca atcgggtaga tctcaatctg gcggctggtg gcatcatact
    10741 tgagcgagct ttccggcgac gcgccaaata gggtgaaatc attatcctgc ataaaaaaca
    10801 tgtacgggct gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg
    10861 gcagagagaa acggcgagat ggcaccacct ggaaaatttc tccagcgcga atcgcttttt
    10921 gcaacaaacg cactacgcca ccgaactctt catcgctctg attacattca caacgcatat
    10981 gcggcacgga aaccactggc agcggcggcg cggcttcggt cagttgctga cgtagttcgt
    11041 tcaggcgagc agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa
    11101 tacgggtgct ttttttctga tggtcaatca ccatcagcgt ttcagcgaga taaaaacaga
    11161 aatcagggca gttattttcc gctgacagtt gcggtaaatc ttcaaatccc gccacaaggt
    11221 cataagagaa caggccgccg aagaacattg cttctcgttc ttccttcggt acattcaaca
    11281 gattctgcaa taaacggaaa gcgtcaaaaa ccgaaaggga gcataagcgg gcgtcttcat
    11341 ccagcagtgg actgacaggg gggaagcgca gcacacggca gtttggtgat tgttcacttt
    11401 ccacacccgc aggcagggcg ttatccagta gtgccagcag ggcttcgcca ttgccggaaa
    11461 gtgcctgaat tgtgacggtg tcacctaatg ctgtaatgcg cagcgcactg tctatcagca
    11521 gcaggctttt taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg
    11581 gacgattccc acacaactgg tgaaaaagcg cagtcgggtt gtcgcgataa gcgccttcgc
    11641 aggttagcag ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa
    11701 aaaaaagccc gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac
    11761 tgcccgtttc aggaagtgcg ccaccaacct ttcagtacga aaattgcttt cattgtcgat
    11821 acccttttta cgtgaacttg cgcactagtt aactggttcg atgattaatt gtcaacagct
    11881 catttcagaa tatttgccag acccgttatg atgtcggcgc aaaaaacatt atccagaacg
    11941 ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac
    12001 agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt
    12061 cggcaccctg gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga
    12121 aatttcacgt tcgggactgg cgctgaatct tattcccggc gtggaaattt ccacggtctg
    12181 ggaaaatcat gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg
    12241 tgagttcctc gcgcagcaga cagaacggcg caatcagcgg gcgcagcaaa ttgccgaaag
    12301 acttgaaaaa gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg
    12361 cgcagtgacg cgcggtcatt ttgcgcgttt ccttgtggag tgcggcaaag ccagttcaat
    12421 ggcagatgtc tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg
    12481 gtgtacaata gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc
    12541 tcatcctggg cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc
    12601 cgaacaccac ggcgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgcac
    12661 ccagctggcc acacttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca
    12721 tcagccatgc ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg
    12781 cgtctggcag ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt
    12841 ttttatattc atcctgataa cccgcagcaa cgtctgatca accaggcggt ggagatcgtg
    12901 cgtaaaggcg gggtgattgt ctatccaact gattccggct atgcgctcgg ctgtaaaatt
    12961 gaagataaaa acgccatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac
    13021 tttaccctga tgtgtcgcga tctttctgaa ctctcgacct attcattcgt ggataacgtt
    13081 gcgtttcgtt tgatgaaaaa caacacgccg ggcaactata ccttcatcct gaaggggacg
    13141 aaggaagtgc cacgccgtct gttgcaggag aaacgcaaaa ccatcggtat gcgtgtgcct
    13201 tctaacccga ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg
    13261 ctaatgctgc caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg
    13321 gaaaaacagg tagatttgat tattcacggc ggctatctcg ggcagaaacc aacaacggtt
    13381 attgatctta ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct
    13441 ttcttataag tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca
    13501 ttgattctcc tgttcgacgc ctgtgaaggc gacaccaaaa ggaagctcta tgagcgaaaa
    13561 attacagaaa gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat
    13621 tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt
    13681 tacccctggg ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga
    13741 acaaatttgt cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga
    13801 tccggaaggg cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat
    13861 tgccgtaggt cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga
    13921 actggcaaac cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt
    13981 atttggtcag gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga
    14041 tggtccggca gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta
    14101 caacgtgact ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg
    14161 tgtgcaggtg agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct
    14221 gccacgcggt ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt
    14281 ggagcttccg ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa
    14341 ggctaatcag attcgtcgtg cggtgaaacg tcacagtcag gtgagcggca gtcgtcgttc
    14401 tggcgggcgt aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag
    14461 attaataatc aatccctatt tgcgccttaa caccggcatc aaacgcatgt ctgatggggc
    14521 gcagctcact taccgtatcc gccagttcaa gaatatcccg atgacagcct cgacccgtga
    14581 taatcaccgt ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca
    14641 aatagtcata cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat
    14701 cagcaagcat ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc
    14761 ggttttgtgt atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct
    14821 ccagcagatt gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcccactt
    14881 tttttccgtg accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc
    14941 cattgccggt aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat
    15001 ctactttttc tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat
    15061 tcctggttta cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc
    15121 gcccatcagc cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc
    15181 ttcggtcggg aaggcgcttg cgcgcattgc ggtgcgcgta ccgcctgggt taatgcagtt
    15241 gacgcgcagg cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa
    15301 tttcgacgct gcatatgcac cccagttggc tcgtccctga cgtccaacgc ttgatgaagt
    15361 aaagaccagt gagccggcgt ccgatttgag taataaagga agcagtgcct gggtgagcat
    15421 aaaggtggca ttaacgttga cctgcatgac gtcctgccag acctgcggat tttgttcgct
    15481 cattgggcaa acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata
    15541 attaacgaca atgcgctgtg ccagttgttg gcaattttcg gacgtgcagg tcagcaaatc
    15601 gagaataaac cactgtggct gacgcccagt ttcttcgttt atgtggctgg ctacctgacg
    15661 taatttttct tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat
    15721 cgcggcttca cgaccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag
    15781 taaatcttgt tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg
    15841 ccagtgtact ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc
    15901 tggggcgata acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg
    15961 cgagacggct taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg
    16021 ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg ctaaaatcgt taccgttgtg
    16081 ctagcgattg cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag
    16141 cgtggcgagt tacgggtcaa caatctcagc gaacagtata aggagatgaa agaagaactg
    16201 gccgcggcgc tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag
    16261 cacaagcaag aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac
    16321 agtaaacctc gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac
    16381 tcgctacgtg aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg
    16441 ctccgtctgg aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg
    16501 cagcgtctgc gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc
    16561 ggcggttaca tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg
    16621 ggttccattg gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat
    16681 attgatatcg aactgcacac cgccgggcag tataagcgca cgctgacctt gctgggtgaa
    16741 aataccgaag aagggcggga gaaattccgc gaagagctga acgaaacgca tcagttattt
    16801 aaagattttg tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa
    16861 cactggtacg gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat
    16921 gaagttattc ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt
    16981 aaacgactca ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta
    17041 cgctggtggc agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct
    17101 cgcgttttgc tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg
    17161 aacatattaa acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag
    17221 tcgccggtaa ataccagcac gccattacgc tgcgtgacgc cggtggcttc aatccctgcg
    17281 agcgtatcct catgctcacg aatgattttg ttggcctctt tcaacagcgt ttcgcggtcg
    17341 atgggttgtg tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag
    17401 gcatggcttg cagacaaata taccacgctg gtggcaagag cgccttactg gcaactttgg
    17461 attttgcatg ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt
    17521 caatctggca atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt
    17581 aggccaagac ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggt
    17641 tgatatccgc agagagcgag tccatatcgg taactcgttg ccagtggaag gtttatcaac
    17701 gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc
    17761 atcgttgagt ccccggcaaa agccaaaacg atcaacaagt atctgggttg tgactacgtg
    17821 gtgaaatcca gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag
    17881 agtgccgact ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct
    17941 ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg
    18001 cctggtaaag agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc
    18061 tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg
    18121 attggtggtg atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg
    18181 atccgccagg catttaacaa accgggtgag ctgaatattg atcgtgttaa tgcccagcag
    18241 gcgcgtcgct ttatggaccg cgtggtgggg tatatggttt cgccgctgct gtggaaaaag
    18301 atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg cggtccgcct ggtggtagag
    18361 cgtgagcgtg aaattaaagc gttcgtaccg gaagagttct gggaagtcga tgccagcacg
    18421 actacgccat ctggtgaagc gttggcgttg caggtgactc atcagaatga caaaccgttc
    18481 cgtccggtca acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac
    18541 agcgtgctgg aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc
    18601 tctacgctgc aacaagctgc cagcacccgt cttggttttg gcgtgaaaaa aaccatgatg
    18661 atggcgcagc gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac
    18721 ctgagtcagg acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa
    18781 tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa
    18841 gcgattcgtc cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat
    18901 gcgcagaaac tgtaccagtt aatctggcgt cagttcgttg cctgccagat gaccccagcg
    18961 aaatatgact ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt
    19021 cgtattttgt gctttgatgg ctggacaaaa gtgatgcctg cactgcgtaa aggcgatgaa
    19081 gatcgtatct taccggcagt cgataaaggc gatgctctga cgctcgttga actaacacca
    19141 gcccagcact ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagaactg
    19201 gaaaaacgtg gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat
    19261 cgtggctatg tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc
    19321 accgatcgcc ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg
    19381 gaaaacagcc tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac
    19441 ttcttctcgg atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt
    19501 atgcgcccga accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg
    19561 gggattcgca cagcgagtac cggggtattc cttggctgtt ctggctatgc gctgccgccg
    19621 aaagagcgtt gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg
    19681 gaaggcgaag atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc
    19741 acggcgatgg acagctatct catcgatccg aaacgtaagt tgcatgtctg tggtaataac
    19801 ccaacctgcg acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc
    19861 ccgatcgttg agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt
    19921 aaatacatgg cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt acgtaacggc
    19981 gaagtggcac caccgaaaga agatccggtg ccattacctg agctgccgtg cgaaaaatca
    20041 gatgcttatt tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc caacactttc
    20101 ccgaaatcgc gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt
    20161 ctgccggaaa aactgcgtta tctggccgat gcgccacagc aggatccgga aggtaataag
    20221 accatggttc gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga
    20281 aaggcgactg gctggtcagc attttatgtt gatggcaaat gggttgaagg aaaaaaataa
    20341 cctttaattc tgtcaggttt ttataaacaa agggtcgcga aagcggccct tttttattgc
    20401 atattatttt ttcttcacac ctatacacta aggctataaa tgatatagtg gttatagtta
    20461 gcaccttttt tattattaaa tcgtattagt cacccgccag gtgtgacgaa aaaacgatgt
    20521 tctgatggcg tctaagtgga tggtttaaca tgaaattaca acaacttcgc tatattgttg
    20581 aggtggtcaa tcataacctg aatgtctcat caacagcgga aggactttac acatcacaac
    20641 ccgggatcag taaacaagtc agaatgctgg aagacgagct aggcattcaa attttttccc
    20701 gaagcggcaa gcacctgacg caggtaacgc cagcaggaca agaaataatt cgtatcgctc
    20761 gcgaagtcct gtcgaaagtc gatgccataa aatcggttgc cggtgagcac acctggccgg
    20821 ataaaggctc gctgtatatc gccaccacgc atacccaggc acgctacgcg ttaccaaacg
    20881 ttatcaaagg ctttattgag cgttatcccc gcgtttcttt gcatatgcac cagggcccgc
    20941 cgacacaaat tgctgatgcc gtctctaaag gcaatgccga tttcgctatc gccacagaag
    21001 cgctgcatct gtatgaagat ttagtgatgt taccgtgcta ccactggaat cgggctattg
    21061 tagtcactcc ggatcacccg ctggcaggca aaaaagccat taccattgaa gaactggcgc
    21121 aatatccgtt ggtgacatat accttcggct ttaccggacg ttcagaactg gatactgcct
    21181 ttaaccgcgc agggttaacg ccgcgtatcg ttttcacggc aacggatgct gacgtcatta
    21241 aaacttacgt ccggttaggg ctgggggtag gggtcattgc cagcatggcg gtggatccgg
    21301 tcgccgatcc cgaccttgtg cgcgttgatg ctcacgatat cttcagccac agtacaacca
    21361 aaattggttt tcgccgtagt acttttttgc gcagttatat gtatgatttc attcagcgtt
    21421 ttgcaccgca tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc tccaatgaag
    21481 aaattgaggt catgtttaaa gatataaaac tgccggaaaa ataatttctt gcgttatttt
    21541 cggcaccttt tatgtagcga aggtgccgga atatattctc ttttgttact tatttctata
    21601 ccgtgcacaa tatttttatc cctcatattt atagggtaaa ttacctgtaa agtagtgtct
    21661 aattacccgc ctcgcaaatt ccagttttca aatatctatt tcaggtcaaa agattgaatt
    21721 attcactgga gacgattcgt aaattcgctg gatttttgac taaagtttct ttaggatttg
    21781 tctcatcgta tgattaatta caccaatcat tcaacgtctt gctgataagt gatggcgatt
    21841 atatgaggtt agcaatgcct tctggaaatc aggaaccccg ccgtgatcct gaattaaaac
    21901 gtaaagcctg gctggcggtt tttcttggtt ctgcactttt ctgggtggtt gtcgcactgc
    21961 tgatttggaa agtgtgggga taactatggt cggtcaggag caactggagt cgtcaccatt
    22021 atgccagcat agtgacaatg aaccagaaac gaaacgggaa tgttccgtcg ttattccaga
    22081 cgactggcaa ctaacatcgc agcagcaagc ctttatagaa ctgtttgctg aagatgatca
    22141 gccgaaacaa taattatcat cattcttatt acccattttt aatgaattaa agggctttta
    22201 atacaccgca gcaataacag cttgagttat ctcaacacaa aataataacc gttaagggtg
    22261 tagcctatga tcaacacaaa tatgaaatat tggtcctgga tgggcgcgtt ttctctgtcg
    22321 atgctcttct gggccgaact cctctggatc attactcact gatccttgac cccgctgcgg
    22381 cggggttgtc atttgctttg ccacaaggtt tctcctcttt tatcaatttg ggttgttatc
    22441 aaatcgttac gcgatgtttg tgttatcttt aatattcacc ctgaagagaa tcagggcttc
    22501 gcaaccctgt cattaaggag gagctatgtc gtcaacccta cgagaagcca gtaaggacac
    22561 gttgcaggcc aaagataaaa cttaccacta ctacagcctg ccgcttgctg ctaaatcact
    22621 gggcgatatc acccgtctac ccaagtcact caaagttttg ctcgaaaacc tgctgcgctg
    22681 gcaggatggt aactcagtta ccgaagagga tatccacgca ctggcgggat ggctgaaaaa
    22741 tgcccatgct gaccgtgaaa ttgcctaccg cccggcaagg gtgctgatgc aggactttac
    22801 cggcgtacct gccgttgttg atctggcggc aatgcgcgaa gcggttaaac gcctcggtgg
    22861 cgatactgca aaggttaacc cgctctcacc ggtcgacctg gtcattgacc actcggtgac
    22921 cgtcgatcgt tttggtgatg atgaggcatt tgaagaaaac gtacgcctgg aaatggagcg
    22981 caaccacgaa cgttatgtgt tcctgaaatg gggaaagcaa gcgttcagtc ggtttagcgt
    23041 cgtgccgcca ggcacaggca tttgccatca ggttaacctc gaatatctcg gcaaagcagt
    23101 gtggagcgaa ttgcaggacg gtgaatggat tgcttatccg gatacactcg ttggtactga
    23161 ctcgcacacc actatgatca acggccttgg cgtgctgggg tggggcgtgg gtgggatcga
    23221 agcagaagcc gccatgttag gccagccggt ttccatgctt atcccggacg tagtgggctt
    23281 taaacttacc ggaaaattac gtgaaggtat taccgccaca gacctggttc tcactgttac
    23341 ccaaatgctg cgcaaacatg gcgtggtggg gaaattcgtc gaattttatg gtgatggtct
    23401 ggattcacta ccgttggcgg atcgcgccac cattgccaat atgtcgccag aatatggtgc
    23461 cacctgtggc ttcttcccaa tcgatgcagt aaccctcgat tacatgcgtt taagcgggcg
    23521 cagcgaagat caggtcgagt tggtcgaaaa atatgccaaa gcgcagggca tgtggcgtaa
    23581 cccgggcgat gaaccgattt ttaccagtat gttagaactg gatatgaatg acgttgaagc
    23641 gagcctggca gggccaaaac gcccacagga tcgcgttgca ctgcccgatg taccaaaagc
    23701 atttgccgcc agtaacgaac tggaagtgaa tgccacgcat aaagatcgcc tgccagtcga
    23761 ttatgttatg aacggacatc agtatcagtt acctgatggc gctgtggtca ttgctgcgat
    23821 aacctcgtgc accaacacct ctaacccaag tgtgctgatg gccgcaggct tgctggcgaa
    23881 aaaagccgta actcttggcc tcaagcggca accgtgggtc aaagcgtcgt tggcaccggg
    23941 ttcgaaagtc gtttctgatt atctggcaaa agcgaaactg acaccgtatc tcgacgaact
    24001 ggggtttaac cttgtgggat acggttgtac cacctgtatt ggtaactctg ggccgctgcc
    24061 cgatcctatc gaaacggcaa tcaaaaaagg cgatttaacc gtcggtgcgg tgctgtccgg
    24121 caaccgtaac tttgaaggcc gtatccatcc gctggttaaa actaactggc tggcctcgcc
    24181 accgctggtg gttgcctatg cgctggcggg aaatatgaat atcaacctgg cttctgagcc
    24241 tatcggccat gatcgcaaag gcgagccggt ttatctgaaa gatatctggc catcggcaca
    24301 agaaattgcc cgtgcggtag aacaagtctc cacagaaatg ttccgcaaag agtacgcaga
    24361 agtttttgaa ggcacagcag agtggaagga aattaacgtc acacgatccg atacctacgg
    24421 ttggcaggag gactcaacct atattcgctt atcgcctttc tttgatgaaa tgcaggcaac
    24481 accagcacca gtggaagata ttcacggtgc gcggatcctc gcaatgctgg gggattcagt
    24541 caccactgac catatctctc cggcgggcag tattaagccc gacagcccag cgggtcgata
    24601 tctacaaggt cggggtgttg agcgaaaaga ctttaactcc tacggttcgc ggcgtggtaa
    24661 ccatgaagtg atgatgcgcg gcaccttcgc caatattcgc atccgtaatg aaatggtgcc
    24721 tggcgttgaa ggggggatga cgcggcattt acctgacagc gacgtagtct ctatttatga
    24781 tgctgcgatg cgctataagc aggagcaaac gccgctggcg gtgattgccg ggaaagagta
    24841 tggatcaggc tcaagccgtg actgggcggc aaaaggacct cgtctgcttg gaattcgtgt
    24901 ggtgattgcc gaatcgtttg aacgaattca ccgttcgaat ttaattggca tgggcatcct
    24961 gccgctggaa tttccgcaag gcgtgacgcg taaaacgtta gggctaaccg gggaagagaa
    25021 gattgatatt ggcgatctgc aaaatctaca acctggcgcg acggttccgg tgacgcttac
    25081 gcgcgcggat ggtagccagg aagtcgtacc ctgccgttgt cgtatcgaca ccgcgacgga
    25141 gttgacctac taccagaacg acggcatttt gcattatgtc attcgtaata tgttgaagta
    25201 acaagtattt gcttgccggt tattttgctt ccggcaagca aatgaattac acgatgcaag
    25261 agggttattt gttcagcaaa tggcccattt tctcggcttt ggtatcgaga taatgttcgt
    25321 tattggggtt acgacctaca atcaatggta cgcgttcaac aatattaatc cctgcttcgg
    25381 tcagaatttc gacttttttc gggttattgg ttaacaagcg gacttcattg acgccaagga
    25441 gtttgaacat atcagcgcaa agagtgaagt cgcgctcatc agcggcgaag cctaactggt
    25501 ggttagcctc tacggtatcg taaccttgat cctgcagtgc gtaagcgcgg attttattca
    25561 gcagaccaat gttacgacct tcctgacggt gatacagcaa aataccacgg ccttcctcgg
    25621 caatttgcgt caatgccgct tcgagctgga agccacaatc gcagcgcaag ctgaacaggg
    25681 cgtcaccggt cagacattcg gaatggacgc gcgcaagtac cggggtatgc ccggaaatat
    25741 cgccatagac tagcgcgaca tgatcgtgtc cggttgccag ttcttcaaat cccaccatca
    25801 ggaaatcgcc ccatggggtt ggcagtttgg cttctgccac acgtttaagc tgcatgaaat
    25861 tctccagata atgctggttc tgtattggct tattttgcca taacgagaag gggttcacct
    25921 aatcacgacg cgtcgatcgt tcacggaatg gcacaaatct gtcaattttt cttggaactg
    25981 gcattttcag ttatgattgt gggacttatc aaaaaggaga ggccatgcgt tcgattgccc
    26041 gacgtaccgc agtgggagct gcactattgc ttgtcatgcc agtagccgta tggatttctg
    26101 gctggcgttg gcaacctgga gaacaaagtt ggctactaaa agcggctttt tgggttactg
    26161 aaactgtcac ccagccctgg ggcgtcatta cacatttgat tttgttcggc tggtttctct
    26221 ggtgtctgcg ttttcgcatt aaggctgcct ttgtattatt tgccattctg gcggcggcaa
    26281 tccttgttgg acaaggcgtt aaatcctgga tcaaagacaa agtacaggaa ccacgacctt
    26341 ttgttatctg gttggaaaaa acacatcata ttccggttga tgagttctac actttaaagc
    26401 gagcagaacg cggaaatcta gtaaaagaac agttggctga agagaaaaat attccacaat
    26461 atttgcgttc acactggcag aaagagacgg ggtttgcctt tccttccggt cacacgatgt
    26521 ttgctgccag ttgggcactg ctggccgttg gtttgctgtg gccgcgtcgg cgaacgttaa
    26581 ccattgctat cttgttggtc tgggcaacgg gagtcatggg aagccgcctg ctgctcggga
    26641 tgcattggcc acgcgatctg gtggtagcta cgttgatttc gtgggcgctg gtggcggtgg
    26701 caacgtggct tgcgcaacga atttgtgggc cattaacacc acctgcggaa gaaaatcgcg
    26761 aaattgcaca acgagaacaa gaaagttaac gctggttgat tttccgaatt taggccttaa
    26821 atcatcaaca atgcgcgtgg atgccatttc gcagacggcg cgaaaatggt actttaaagg
    26881 gctattgcgg taagttgacc ataatttatt cgctctaacc acataacggg aagtaatgtg
    26941 aaatatttac tcattttctt actggtgtta gcgatcttcg tgatttcggt cacgttgggt
    27001 gcgcagaacg atcaacaggt gacgtttaat tatctgttag cgcaagggga gtaccgtatt
    27061 tccacattgc tggcggtatt gtttgctgcg gggtttgcta tcggttggtt gatttgtggc
    27121 ctgttctggc tgcgagttcg tgtttccctg gcgcgcgctg aacgtaaaat aaagcgactg
    27181 gaaaaccagc tttcacccgc gactgacgtg gctgtagtgc cgcactcgtc agcggcgaag
    27241 gaataacttt ctatgctgga gttgttgttt ctgcttttgc ctgtagccgc tgcctatggc
    27301 tggtatatgg gccgcagaag tgcgcaacaa aacaagcaag atgaagccaa ccgcttgtcg
    27361 cgtgattacg tagcgggggt taacttcctg cttagtaatc aacaggataa agcggtagac
    27421 ctgtttctcg atatgcttaa agaggataca ggcaccgttg aagcccacct tacgctcgga
    27481 aacctgttcc gttcgcgtgg cgaagttgat cgcgctattc gcatccatca gaccctaatg
    27541 gaaagcgcct cgctgaccta tgaacagcgt ctgttggcga ttcaacaact ggggcgtgat
    27601 tacatggccg ccgggttata cgaccgcgcg gaagacatgt tcaatcagct gaccgatgaa
    27661 actgacttcc gcattggcgc gctgcaacag ttgctacaaa tctaccaggc taccagtgag
    27721 tggcagaaag caattgatgt tgccgaacgc ctggtgaagc tgggtaaaga taaacagcgc
    27781 gtcgaaattg cccatttcta ctgtgagtta gccctgcagc atatggccag cgacgatctc
    27841 gatcgtgcca tgaccttgct aaaaaaaggg gcggcggcag ataaaaacag cgcccgcgta
    27901 tccatcatga tgggacgcgt gtttatggcg aaaggagaat acgccaaagc cgtcgaaagt
    27961 ttgcaacgcg tcatatccca ggacagagaa ctggtcagcg aaacgctgga aatgttgcaa
    28021 acctgctatc agcagttggg taaaactgcc gaatgggcag aattcctgca acgcgcggtg
    28081 gaagagaaca ccggtgccga tgctgaattg atgcttgctg atatcatcga agcgcgcgac
    28141 ggtagtgagg ctgcacaggt ctatattaca cgccagcttc agcgtcatcc gaccatgcgt
    28201 gtattccata agctaatgga ttaccactta aatgaagcgg aagaagggcg cgccaaagag
    28261 agtctgatgg tgctgcgtga catggttggc gagaaggtgc gtagtaagcc tcgttatagc
    28321 tgccagaaat gtggttttac tgcatacacc ctctactggc attgtccgtc ttgtcgtgcc
    28381 tggtcaacta ttaaaccgat tcgcggtctt gatggcctgt aatttttaaa aaaatccgac
    28441 tttagttaca acatactagt tattgatgtc ccattgtgct ccggcaacga cggagcagaa
    28501 aaagcctgct aggggaaaaa tcgcaactgt taatttttta ttttcacggg tagaatgctc
    28561 gccgtttacc tgtttcgcgc cactcccggt gcccatcatc aagaaggtct ggtcatgacg
    28621 ttaactgctt catcttcttc ccgcgccgtt acgaattctc ctgtggttgt tgcccttgat
    28681 tatcataatt gtgatgacgc gctgtccttt gtcgacaaga tcgacccacg cgattgtcgt
    28741 ctgaaggtcg gtaaagagat gtttacattg tttgggccac agtttgtgcg cgaacttcaa
    28801 cagcgtggtt ttgatatctt tcttgacctg aaattccacg atatccccaa cactgcagcg
    28861 cacgctgtcg ctgctgcagc tgacttaggc gtgtggatgg tgaatgttca tgcctccggt
    28921 ggggcgcgta tgatgaccgc agcgcgtgag gcactggttc cgtttggcaa agatgcaccg
    28981 cttttgattg ctgtgacagt gttgaccagc atggaagcca gcgacctggc cgatcttggc
    29041 gtgacactgt cacctgcaga ttatgcagaa cgtctggcag cactgacgca aaaatgtgac
    29101 cttgatggtg tggtgtgttc tgctcaggaa gctgtgcgct ttaaacaggt attcggtcag
    29161 gagttcaaac tggttacacc gggcattcgt ccgcagggga gtgaagctgg tgaccagcgc
    29221 cgcattatga cgccagaaca ggcgttggcg gctggtgttg attatatggt gattggtcgc
    29281 ccggtaacgc aatcggtaga tccagcgcag acactgaaag cgatcaacgc ctctttacag
    29341 cggagtgcat gatgagtgat tccaacagcc gtctggtcta ctcaacggaa accgggcgta
    29401 ttgatgagcc caaagcggcc ccagtacgcc ctaaaggcga tggagcggtg cgtattcagc
    29461 gtcagaccag tggacgtaaa ggtaagggcg tttgcctgat taccggtatc gatctcgatg
    29521 atgccgaact gacaaaactt gcagcggaac tgaagaaaaa atgcggctgc ggcggagcag
    29581 ttaaagatgg agttattgaa atccagggcg ataagcgtga tttattaaaa tcactacttg
    29641 aagcgaaagg gatgaaggta aaactcgcag gcggttaaca taaaaagcca cggatatatc
    29701 cgtggctttc gaatatttta ctgtgtgtat tattcagtta tttcgtactg aacagaatca
    29761 agcgtattat aattgcgagt cgataatcga catttattta cgattattta ccgacctggt
    29821 gaccaataac accaccgaca gctgcaccac ctaatgtacc caacgtactg ccatcggtca
    29881 gtactgcacc gcctaatgcc cctgcacccg cgccgattgc ggtgttgcgg tcccgtttag
    29941 accagttaga acaggcactc agagacattg ccaaagtaat tgccagaaca gccgcggtca
    30001 tttttttgct cgttacaaac ataatactct ctcctgaatt tatgattcac ggaagtaagc
    30061 tctctataac tataatagct aagaataagc ctggtgaaat tattccgtga aatctgctcg
    30121 cgcgcagggt tatatcacgc aggtgataat tacttcctgt tatatcgctg ataataattt
    30181 tatatcttga gagtgttaat aacaggtaaa tagtcttaat tatcaaccag gaatcatctt
    30241 agagcggatg atttgccaaa ctgcaaatca tccgtagaga agggaaatgg ttaaatgtca
    30301 acgacgtgaa cggtgagttt actgtgctca agatgcatca gatcgctggc gcgtattttc
    30361 gaatcggtaa tcacgcgatt gaatcgctca acgggaccga tggagtatga atgtacagca
    30421 ccaaatttcg agctgtcagt caggactatc gcttcgcact ctttttccag cacggcattg
    30481 accacatcgg tgcgcatcat atcgcgaccg gtgaatccag tttcaggttg ccagccatca
    30541 ataccaataa atgctttgct gaaatgtacc tgctggatgc actggcgtgt caaagggcca
    30601 accatacttt cgcttttttt ctggtacacg ccaccgagca aaatgacttc acaaggcgcg
    30661 tctttcagca aatgcgcgat gtagctgctg accgtgatga tagtgacatt tttcttctgc
    30721 tcccccagag tccgggcgag cagggcattg ctgctgccat tttcgataaa gatggtttcg
    30781 cccggttgaa ccagtgacgc ggcaaactcg gcgagttcac gcttcagcgt atagttgctc
    30841 atcatacggg tttcgacgtc atcactatca agcgaaactg caaagccatg tgcacggcgg
    30901 aggtaactca gtttttcgag ggtgttgaga tcctggcgaa tggtaacttc agaaactcca
    30961 gtggcttttg ccagatcggt tacgctaacc tgaccctggt caatgaccat ctgtagaata
    31021 gtttgttgtc gggaattcat cgctgtaatt cttataacgt tataatacta aataaaaaaa
    31081 tactgacgcg ccgggcatcc tcaaatagga aaacctgaag gcacgattgt taaatctccc
    31141 acggtgtctt cgggatgtct ggcgtagatt cagcaacctg gcgcaagagt tctgctttaa
    31201 gtatctcgag attatggatc gcagagtggt aatcgcctgc cacaaggata tctaacacaa
    31261 tatcaatacg ttcggcaacg cgctgggcat caaattcaga catcaaggac atccttatag
    31321 caaaaggggg aaaatgaata atgcaaaaat tgccgctaaa agagaagtgt ttaacagcaa
    31381 cggctaatta tcatccagga atacgatata taatgacggg atatagcgct aagtatatat
    31441 attcatctac ttatgcgcgc ttcagatagc gtttatacca gcgttcgaag gcgacggcgg
    31501 gcatcggttt ggcaaacaaa aatccttgcc gctcattgat cccgttcttg gttaaaaaag
    31561 catcttcctt tgcactctct acaccttcgg cgatcacctg aagattcaat gcctgggcca
    31621 cagcgacgat cgcccggacc agtgactgcg agacaggttg tttgtgaata tctcgaacaa
    31681 aaacctggtc aagtttgatg gcatcgatcg gaaagcgcgc cagttgcgaa agtgaagagt
    31741 agccggtacc aaaatcatcc agatgcactt gcgcacctag ttggctaaat tgttgaataa
    31801 cagacagtgc cagttcatca ttctcaatca gacaactctc tgtcagttca acatctatag
    31861 ggcagtattc aaaattgagt tcctggagaa cctgtttcag ggcggtgaaa atggtttgat
    31921 cggcgagctg acgtgcagaa atatttaccg ccactcgcag gtttatgcct ttatcccgcc
    31981 actttgccac ctggcgtacg acatcgagaa tcacccaacg gcctaaaggc acaattagcc
    32041 ctgactcttc ggcgtaggaa atgaagtcca acggtggaat caacccacgt tcaggtgact
    32101 gccaacgtac tagtgcttcc agactgcgca cttcgccacg ctaggtgatt ttcgataacc
    32161 aactgatcgt tttccagtgc tttacgcaag ttggtatcca gccagagata ttcaaatacc
    32221 cgctgattca tttctggagt aaaaacgcaa aattgtcctc gtccgccttc cttcgctgtg
    32281 tacattgctg tgtcggcgtg acgaataata gtcgcgctgt ccgaaccatg ttcgggagag
    32341 agcgaaatac ctactgaaca gctggtataa acttcaatta aaccaatacg aaaggggagg
    32401 cgtaagcggg tcaaaattcg tgatgccatt gcttccagcg cgctttgtga ggtattggat
    32461 gccagtacca gaaactcatc gccacctgga cgcgccaaca cctggtcatg ttcgagacag
    32521 cttaaaatag ccaatgacac gtcgcgtaat aactggtcac caaacaaatg cccataggcg
    32581 tcgttgacct ttttaaaatt atccaaatca agatacacaa ccccaacttt attgttatct
    32641 gcctgattaa tagcgtgatc gattaactcc tgcattgcgt tacgattcgg cagtccggtg
    32701 atactgtcgg tatttgccag aatacgcagt cgctcctgag tgcggcgctc ttcggtaatg
    32761 tcggtaccgg aacagattaa aaaaatctcg tttttgccac tgccgctgtg gacaaattta
    32821 ttgcgaaaca gaaacagccg ctggccttta cgtgttggta tccacagttc gacttcatat
    32881 gcattgccgc ttcgaaaaaa tacacggtta ttgcgcctgg atgccgcagc ttcacgacgg
    32941 ctcataaaca gtttaaacac gctttaccca atgacgtcgt gttctttcaa ccctgtgtaa
    33001 tcttcacata accgattgaa gcgttggata ttcccccggc tgtcgagaat cactattgcg
    33061 gaatgtgctt cagaaactac ctgctcagca aaggaaagcc cgtgtgataa atcacgagca
    33121 accgccggtg tgtcatgcca tgcagacgcg ttgccagccc attcccgttt attgattttt
    33181 cgtcctacca gatgcacaga aagatcgctt tcatcgacgg tcagactcat catcaggctg
    33241 gaggtgatga ccgtcatttc cctgatgcga gcagcctgtt cggcagataa ctgcaatgta
    33301 cgatcaggtt ctgtggtttt ggtggtagaa aagcgcaaag catcgctgct ttccgtcaac
    33361 cgccagtatg gattgtgcga tccgagaaag ttgtacaacg ttgtggactc cctaacggtt
    33421 ttcatgatgc atatcctccg gttaacagcg gagtccgcgg ttaaataaaa ggaacaacat
    33481 gacttacatg aaattaacgg cggctaaacg cattgttttt tatatattta tttgtaatcc
    33541 agttttggaa aaacgccagt tttcaaacga aagtcagtta aaaaatctgc ctggatataa
    33601 cgaaggtaga gcggggaaat aaacggccca tccatgagga atgggccgtg aaaggagatt
    33661 acgcgaccgg gcgcgcaata atgctgcggg tttccatgcg gacttcggca atggtgacgt
    33721 caataacgtc agttactttg taaaccgttt cacctttaat ttgtacagtg ccgttttcct
    33781 ggctgcaaac cagttcatcg cgcacagcgt gtaagaaagg tgccggaata aaggcgatag
    33841 cgccgttatc aaccaaacgc acacgcatgc cgccacggct gatatcgaca atttccgccg
    33901 cgaaacgggt gccggtcccg gctttatctt tcaggaagcg tgcgtataac cagtcgccaa
    33961 catcacgttc tgccatccgg ttgagacggc gacgttcggc catttggaca gtgatttcat
    34021 cctgcggacg cgtcgcagtt tcgcctttga taaccgcttt cagcagacgg tggttgatca
    34081 tgtcgccata tttacggatc ggcgaagtcc aggtggcgta tgcttccaga ccgaggccaa
    34141 agtgaggacc gggttcagag ctaatttcag caaatgactg gaagcgacga atgcggctgt
    34201 cgaggaaacc agttggttgc gcatccagtt cacgacgcag tttgcagaaa ccgtccagcg
    34261 tgagcacttc ttcggcatcg acatgcagac cgtgcgtttt cagcaacgct gccagcgcgt
    34321 cggcattcgc cggatcaaag cccatatgca cgttatagat gccaaaaccg agcttatcgc
    34381 gcagtacgcg ggccgcacaa atgttagcgg caatcatcgc ttcttcgacg atacggttgg
    34441 caatgcgacg aggctcggcg acgatatcca gcacttcacc tttttcaccg agaataaagc
    34501 ggtaatccgg gcgatcttta aacaccagtg cgtggttatg acgccactcg ccgcggcgtt
    34561 ggcaaatttg cgctagcaaa cggacttgtt cggcaattgc ttcactttca ggcttccagt
    34621 caccggtatt ttccagccag tcagaaacct ggtcatacac cagcttcgct ttggattcga
    34681 tggtggcggc aaagaattcg atattatctt cagtggtgcc atcagcggag agcgtcatgc
    34741 ggcatgccag taccgggcgg acttcattgg cgcgcagtga gcagagatcg tcagaaagct
    34801 cgcgaggcag catagggatg ttgaagccag gcagatagtt ggtgaatgcg cgaattttcg
    34861 cggctttgtc cagcttgctg ccttcagcaa tccacgcggt tggatcggca atcgccacaa
    34921 tcagctgaag tttgtcatcc ggcagcgcct tagcgaaaag ggcgtcatcc atatcttcag
    34981 tgctggcact gtcgatggtg acaaaatcca gcgaggtcag atcttcacga accagacctt
    35041 catcgagcat ttcggtagcg acgccgtctg gtgcttcttt ttccagatta tggcgcgcaa
    35101 gggtaaccca ccacggtaca aagtggtcgt caccaaaagt gatgtattgt gtcagttcag
    35161 catagaaaga acgatcgcct ttcagcggat gacggcgcat ttcggcaacc gcccagtcgc
    35221 cttctttaaa ctcgtggttc aggccacggg ctgcgcggca aggaatggcg tcttttaaaa
    35281 gtggatgatc aggaacgatg gtcagacggt catttttgcc ctgaacttta cccacgaaac
    35341 gagtcaggaa cggttcaacc agttcttctg gctctgcgga ttcacgttct ttttcactgt
    35401 ggatcaccgc gataattcgg tcgccatgca tgactttttt catctgcggc ggcggaatga
    35461 aataactttt ttgtgcgtcg acttccagga agccaaagcc tttttctgtg gcttttacca
    35521 ccccttcagc gcgtggcgtc tgggaatgca gttgctgttt aagctgcgct agcagcgggt
    35581 tgtcctgaaa cataattgtc tattttggtg gccattagag cggctgacag ttttacgcga
    35641 atctgtctga cgcggcaagg ttaatatgtc tcacccaacg cgatttttaa gcgatttatc
    35701 cagccacaca gcccgctcca taccagcaga ttaataatct gcgttgatga tttttcgtgt
    35761 tcgagtaaag ggctgaactg ggcggcgcta aagcgatcgg gtgctcgggt cagccattgt
    35821 atcgcctgaa cggtagcgcg ctcaacagaa tgacggtcag cccatcggtt aatttcattt
    35881 tcgcccttgc gcagaagggt gctgattttt ttatattcat ttgatgaatc catacaggtg
    35941 gaaaaacaag caggactgcc atttatacgc gcgcataaca acgccgcaag ctgattatcc
    36001 ggctgtgcat tgacccgtgt gctgtttaac aacttttcga gaagggaaag tgaaataggg
    36061 tggagagagt gtgtttcggc cagttggcaa agttctgcag ttgaactttt tgtgtgctct
    36121 tcaggtagtt tttccacctc atagctgctt cgccagcgta ttgattcatc agcaaacagt
    36181 gacgcgtcgg cgtagttttg tatcgccagc ccgggtaacc agcgtaccgg atgcccgaga
    36241 taagcctgaa atgtcgcaat tgtccgcgcc tgaaagccaa taaaaccaat gatttggtta
    36301 atgatcacac agtcccagac agtaagaccg acgtcattca gatgctggcg cgatcggttg
    36361 tcgataatag aaggcgagct agccagttgg cgggcatatt gcgtgatttg tgccaaccga
    36421 tgattacttt ccctggagga atcaggtccg gggagcggcg taagtcgcgc cgaatagtag
    36481 ttacataacc gctggacgcc gcaaacctga gcaaccgtca gggccgtcga caagcgttca
    36541 taagcggtga aggtttgtga acgggtcacg gtaagctggt cagggaacaa ctccactgca
    36601 agaacgcgcg cagggaccag ccatgaaagg aagggggcca gcgtctcttc atcaaggatc
    36661 acgtcgagca gaaagggatc gctgaccttt gcggcttccg ggaccagagg cagaacgtca
    36721 tactccgtag tactggattg cgtttcatga taccagtggc ttttgccggt gatgtggcgt
    36781 tgttccatgg gcgctccttg gtcgtaaagg aaatcgttat cctgacgcaa ggcgggaagg
    36841 ggagaaagac ggatcggcga taacaaatat cagaaaggta taacagagat aacgggcggc
    36901 agaacgccgc ccatctttac caacagaacg attatttcag ttcgagttcg ttcattgcag
    36961 cgatgctgaa accgccgtca acgtggacta cttcaccgga gataccggca gagagatcgg
    37021 agcacaggaa tgccgcagag ttacccacat cttcaatagt aactgtacgg cgaatcgggg
    37081 taacggcttc gcaatgagcc agcattttgc ggaagtcttt gataccggag gccgccagag
    37141 tacggatcgg accagcagag atggcgttaa cacgcacacc ttccggaccc atcgcgttcg
    37201 ccatatagcg cacgttcgct tccagagacg cttttgccag acccataacg ttgtagttcg
    37261 ggatagcgcg ctcagcgcca aggtaggaaa gggtcagcag ggcagaaccc ggattcagca
    37321 tggagcggca agcttttgcc attgcaacga agctgtagga gctgatgtcg tgggcaattt
    37381 tgaagccttc acgggtaacg gcgttaacat agtcaccatc cagctgatcg ccaggtgcaa
    37441 aaccaataga gtgtacgaaa ccgtcaaatt tcggccaaac tttccccagt tcagcgaaca
    37501 tggtgtcgat gctggcatct tctgcaacat cgcactgcag aacgatgtca gaacccaatt
    37561 gagcggcaaa ttcttctacg cggcctttca gtttgtcgtt ctggtaggtg aatgccagtt
    37621 cagctccttc gcggtgcatc gcctgagcga taccgtaggc gatggacagt ttgctggcaa
    37681 caccggttac cagaatgcgc ttaccggaaa gaaaacccat agctttaatc cttattgttg
    37741 atgcttgttg tgcctgaaaa tcaggcgatt cgtcgtttta gtaaacagta cgaacagata
    37801 aacggttatt ataatcaacc tggctgtgag tagctatagt tgccaggtcc gaccggagca
    37861 ggctgcggca gggggggcgc ttttcccctt accctaaccc tctccccaga ggggcgaggg
    37921 gaccatattg tgcaaatatt gttaccccag caacaaacag gctcatacag cccctaaccc
    37981 ttttatggcg atggctggga tggttcagac cttgccgaat attctccagc actgtctcca
    38041 tgttttcacc acaacagcta ttcggctcgg tcggccccct cgccctttca gggtgagggt
    38101 gaccggcggt tcagttcctg cagaataatc tccagcaccg cctcctcatt acaatcaatc
    38161 tcgttattcc agaaacgcag cacggtccag ccctgcgact caagccagct agtgcgcctg
    38221 gtatcatagg caactgccaa atcatgctgc ccaccatcca gctcaacgac tacgcgcgcc
    38281 gagcagcaag caaaatcgag aatgtagctc cccaccggat gttgacggcg aaatttgaaa
    38341 tcaccaaaac gtcggctgcg aagatatcgc cagagctttc gttcctgcaa agtgagattg
    38401 cgtcttaaat cacgcgcatt tgattttatt ttatccatca cctcattctg acaagattta
    38461 atctcttgtc agaatgaggt gaataatctg gaaggaggat tcagaaaatt agcgatcttt
    38521 acgccacgca tccgctgtca atgcttcgcc aaagtgcccg gcaatcagcc gtttggtgag
    38581 ttcatgcagc ggcgatgcca gcacatccgc ggtgctgcct cgctcgacaa cctcgccctg
    38641 atgcatcacc agcacctggt cgctaatgtg cttcatcatt ccgatatgct gggtaacata
    38701 aatatacgaa atgccctgtt tttcctgtaa ctccagcatc agattaatca actgcgaacg
    38761 cattgacata tccagtgaag cgagggcttc atcggcaata atgacttttg ggcgcaatat
    38821 cagcgcgcgc gccagaccca gacgctgttt ttgtccgggt gccaacatat gcggatagta
    38881 actgacgtga tccggtagca gcccaaccat acgcatcgtt tcaataatct gtttgcgacg
    38941 ctgttccggt tccaggtcgg tgttcaggcg cagtggaaaa tccagaattt gtgagatacg
    39001 ttgacgggga ttcaacgagg tcgaaggatc ctgaaaaatc atgcgaatac gctgactacg
    39061 gaaggaataa tcgccaaaat gcagcggatg atcgtcaatc aataactcgc cgctggtagg
    39121 ttctatcatt cccgccagca tttttgccag tgtggattta cccgaaccat tctcgccaat
    39181 aatcgccagt gtctggcctt cacgtagcgt aaagctcaag ggttttaccg cttctacggt
    39241 ctgacgacga aaccaaccgg tccggtagcg gaacgtttta cttaaattac gcacttcaag
    39301 cagcgtttcg atcatctcac tttttctcca tgttcagcgg gaaatgacag gcatagagat
    39361 gatttttcgc ccccgtcaaa cgtggcgtca caatgcattc tcgttgtgca tacgggcaac
    39421 gtggccccag acgacaacca atcggtaact gttccagcaa tgggatagcg ccgggtagag
    39481 tattgaggcg acttttatgc ggcatcgcgc tgccgaagtc tggtatcgcg cggatcagcg
    39541 cctgggtata aggatgatgt ggcatcgtca ccaactcctt actcggcgcg gtttccactg
    39601 tttgaccgca gtaaagcacg ttaattttat ccgcccattg gctaagcatt tgtaagtcat
    39661 ggctgataag caaaatagtg gtattgctgt tttggttgag acgcgtcagc aggcgaaaga
    39721 tttgcgcctg ggttgttggc tccattgagt ttgtcggttc gtcagcaatc agcagacgcg
    39781 gttgattcgc cagtgcaatg gctatcatca ctttctgaca ttcaccttcg gtcaactcat
    39841 agggaaaact gcgcatcgca tctttgtgat ctttaatccc cacgcggtgc agcagttcaa
    39901 tggcacggcg tttgcgccag ccaaaacgct gccaccaacg gcctttatag gtccaggctg
    39961 ggatgttttg catcaactgg cgacccacac gttctgaagg gtcaagacac gactgcggtt
    40021 cctggaaaat catcgacacg ttatggccaa ccagtttgcg ccgttcgcgt gcggagagac
    40081 gcagcaaatc gatatcatca aaacgcatac ggtcagcagt aacacgccag ttatctttat
    40141 tcaccccaca aatcgctttc gcaatcaaac tcttgccgga accggattca ccaacaagac
    40201 cgcggatttc accttcggtt aaggtcatac ttacccggtc gacggcttta acccactcat
    40261 caccggtttt aaattcaatg gtcaggttac gaatatcgag taatggcatt attccacccc
    40321 cgcaataatc gcacgacgaa cgccatcgcc aaggaggtta accaacaaca cgctaatcat
    40381 aattgccgca cctggcagca tgacagtcca cggggcgaca taaatcagtt ccagcgcatc
    40441 accgagcatc gctccccatt caggcgaggg gagttgtgcg ccgagatcga gaaagcccag
    40501 cgcggcgata tcgagaattg ccatcgacag tgcgcgggtg atctcggtga ccagcccggc
    40561 ggtgatgttt ggcatcacag caaaccagag aatattcagc gttgatgcgc catccagacg
    40621 ggcggcgata acgtactctt tttccagttc gtcatgcacc atgctgtaaa tcgaacgtac
    40681 catacgcggc agcaatgcca gccagacagc aaacatagcg tgggacaaac tcggtccggc
    40741 aaacgctaca acgataattg ccaacagcag cgaagggatc gccagtaagg tatccagaat
    40801 atggttaagc accgctgagc ggaggccgtg tgtcgccccg gcgaaggtgc ccagtaccag
    40861 gccacagatc gtcgcggcaa gcgtcaccac aaatgcgcca cccacggttg gcgcagctcc
    40921 gctcagtaaa cggcttaaaa catcacgtcc gaggtcgtca gtccccagga agaaagaaac
    40981 ttcgccatag cgcgaccatg atggcggcaa taattgataa ccgagaaatt gctggtcgat
    41041 gccgtaaggc gcaaaccagc cgccaaaaat acacagtaca gccagtcccg cgcagccgta
    41101 caggccgacc atcgcagagg cgtcactata aaatttgcgc caggcggtac gcagcgtgcc
    41161 cggcgggcgc ttttcgctgt atacgctatc gtaaggcata ccattcctta tgtttcagag
    41221 ggttagccat ggcacccaaa atatcagaaa tcacgttaac aataatgacc agtgagccac
    41281 acaccatcac tccggcggaa atggctgcgt aatcctgctg gcggatggcg ttaattaacc
    41341 agcgccctaa acccggccag ctaaaaacca tttcggtgat catcgccagc gtcagcatgg
    41401 tggaaaactg taagcccagg cgaggaataa ccggcggtaa cgcgttatgc agaacgtggc
    41461 gacgcaaaat agtaaagcgc gacaaaccgc gggtcgccgc cgcttttaca tagttctggt
    41521 catacacttc aatagtgctg atacgcatca ggcggatcac ttcagttgtt ggcgcaaccg
    41581 acagggtgat cacgggcaat atcatatggc ggattgcgct catgatcatt tcatcccgcc
    41641 atggtgagtc cgaaagccag gcatcaatca acgcaaaacc ggtaatcggt ttcacttcgt
    41701 agagcagatc gaaacgccct gaaacgggca accaacctag cgtgagtgaa caaaacagtg
    41761 tcaacagaag cgccagccag aacaccggga ttgaaaagcc cagtaaggcg atggcgttga
    41821 tcaagttatc ctgccactta tggcgcgtaa tcccggcaat catccccacg gggatgccaa
    41881 caatcagcgc aaaaccgaag gcgaggatgc acagttctat cgtcgccggg aagagctctt
    41941 ttaactgttc ggcaattggt tgaccgttaa tgctggaaac accaaaatcc cagtggatca
    42001 ggccattaaa ccagaacacc caggcattcc acagtgacgc gccttgtaac ggcgcgtgtg
    42061 gagtgaaata gctcaagctg aagccaacga aggtcagcag gaacaaggtg acaatcaata
    42121 acaaaatgcg gcgtaaggtg aagataatca tggttttttc acctcatcct gtttctcgcg
    42181 atacacccca gcaaaggagg cgttaccaaa cgggctaagt accagacctt tgatatcgta
    42241 ccgataggcc tgcaaacgca atgacgacgc cagcggcaga ataggtaatt cctgcgccag
    42301 aatactctgc gcttcgtcat aggcttcaat acgcgctgcc agctgctgcg aggagagcgc
    42361 cttacgcaac acgctgtcga atttcggatc gcaccagtgg gcgaggttgg tctgtgaatg
    42421 aattgccgcg cagctcagta acggacggaa gaaactgtcc gggtcgttac tgtccgtcgc
    42481 ccaaccggat aacgtcagat catggctcat atccatcaac cgcgcctcct gaaagcgacc
    42541 ttctaccggc acaatcacca cttttacgcc aacctgcgcc atatccgcct gaatcagttc
    42601 ggcagttttc agtggactgg ggttccacgc ctgcgaacgt gtgggcaccc acagtttcag
    42661 cgttaagttt tccagcccca acgccttcaa ctgttcgcgc gatttcgccg gattgtattc
    42721 agtaatttta gcctcgttgt cataggccca cgaggcgcgc ggtaaaatag aggccgccgt
    42781 ttcagccgta ccataataga tggattgcat caggcgctgg ttattaatcg ccagtgccag
    42841 tgcatggcgg acagcgggat tatttagcgg cggtttggcg gtgttaaatg ccagataggc
    42901 gacgttcatc ccaggacgca gcgttaaacg caagcgcggg tcgtcacgca aaatggatag
    42961 ctggctggca gcaggccagg ccagaacgtc gcattccccg gtcaggagtt tcgacagacg
    43021 tccggtgccg ccggagccta aatccaccac tacctgcggc attaacggtt taccgcgcca
    43081 gaagtcatca tgacgttgta ggcgaataaa ttgcccggcg cggtattccg acaactgata
    43141 cggaccggtg ccgaccggtt gacggtcgag ttgctcctgg cgatcttctt tctctaactt
    43201 ccgggcatat tctgccgaca tgaccgaagc ataatgggtt gcgaggtgcc acaaaaaaga
    43261 agcatccggc tgggcgagtc ggaactcaac ggtatgatta tccagtttgc ggacgctttt
    43321 cacgttatcg gcaaattgca ggctgtcgaa gtaggggaag ttgctgccgt tgacgttatg
    43381 ccacgggttg ttgcggtcaa aaattcgctg gaaggtaaac accacatcgt cggcattcat
    43441 tttacgagtg ggagtaaacc agtcggtttt ttgaaacgga acatcgcgac gcaggtggaa
    43501 gcgataggtc gcgccgttgt cgagtacttc ccagctttcg gcaagttcag gcatcaggcg
    43561 ataggtatag ggatcgacat ccagcagtcg atcataaaac tgggcggcaa gggtatcgac
    43621 aattaaccca ctgctcgctt tggatgggtt aaaggtgttg acttgcccgc tgacgcaata
    43681 gacaaaaccg ctgtcgcgga tatcagcatg cgggggggat tcaggcgcgg cgattgcctg
    43741 accactcaca agtccagcaa tcaccaaaag agacgataat acctggcgca taatattaag
    43801 ggattttatg taaagaggct atcttactaa tatttaatga catttgccat taccgtttgt
    43861 gttcaggggt cgtaatgagg gccgtgtggt tggtcgttga acaggtggcg tcgccatctg
    43921 ttcaacattc gtacccgtga tttctctata actataactc acagaacaac ttagcgagga
    43981 gcagagcggt acgatcgtga gaaacccatt aaagcacgct gcagtgcgtg tcattgttag
    44041 ccggatgcgg cgtgaacgct ttatccggac aacgatactg accgatcgtc tgcaattaaa
    44101 taattactca ttaccccatt gattcaaaaa ctctgcgatc tcatcaatgc gtacgggatt
    44161 aatccccgct tcagcagcca tttcatgttg ggcttcttcg ctgatctctt cattgttcat
    44221 caaacgggtg agcagtaact ggaagtagtg ggccagcggt gtgcgttcag cctctgcgac
    44281 aggctctgcg cattcggtcg cgtactcatc ggcaatatca aaatatttta gcgggatatc
    44341 gtggttcatt atttgcccct ggggttaatg cgctaagcat aagggcggat gatagcactc
    44401 gtgatccggg tcatacatct ttttatagtt aatggcgcgg cgcgttaccc tcaggcagga
    44461 taatgcgccg cgcatccggc tattacgttt cgctcaccgg cgttctgtca ccttcgtaca
    44521 gcggcacttt gcgatagcgg cggatcagat accacaaata tgcgccgcct aacgaagccc
    44581 ataccagacc gagtgtcagt gacgttgact ctaggttaac ccacagcaca cccaccgtca
    44641 gcgcgccaac cagcggcatc agcaaatagt ggaagtgatc cttccagctt ttattcattc
    44701 ctttacgccg ccagaaatga ttaaacaccg acagattcac gaaggtaaac gccaccagag
    44761 caccgaagtt aatcagtgct gtcgcggtga ctaaatcaaa gaacagcgcc gacagcgcga
    44821 caatcccgac cataatgacg ttgagtgccg gagtccgcca ttttgggtgc acatagccaa
    44881 acacgcgctc cggaaacaca ttgtcacgcc ccatcacata cagcagacgc gacacgctgg
    44941 catgtgaagc caggccagac gctaatgtgt tcacaaacgt ggtgcagagg aaaatcgact
    45001 gaaagagctt gccgccgacg tacagcgcaa tttcaggcag tgcggcgtcc gggtctttaa
    45061 agcggctgat atcgggaaag aacagctgca taaagaacga cgccgcgata aagataacgc
    45121 cgccgtatac cgccgtcagg aagatggctt tcgggatcac gcgtgcggcg tccggcgtct
    45181 cttccgaaag cgtggtcacc gcatcgaaac cgaggaacga gaaacagaca atcgtcgcac
    45241 cggtaataat cgggatcagg tgcgcgttct cgctgataaa cggctgaagt gaccagacgg
    45301 tgccaacgcc ttctccttta tgcagtccct gaaccaccag gaagataaac accaccatga
    45361 tggagatttg caccagtaca aacagggtat tgaagttagc gaccaggttg acgctcttca
    45421 gattcgcggc ggttaaaatg gcgacgaagg ttaccaccca cacccacggc ggcacttccg
    45481 ggaagagggc ggagagatag attttcgcca acaaaacgtt gatcattggc aaaaagagat
    45541 aatccagcag tgatgaccag ccgaccataa atccgacgtg cgggttaatc gacttttgcg
    45601 cgtaggtata ggccgaaccg gcctccggaa actggcgaac cagtttgccg tagctgatag
    45661 cggtaaacag cacgcccgcc agcgccagca aataggacgc cggaacgtga ccgtcgctaa
    45721 tgccggagac aataccaaaa gtatcaaata cggtcatcgg cgtgagatag gccagaccca
    45781 tcattaccac ctgccacaat ttcagtgatt ttcgcagacg ggttttgccg ggttgcgcag
    45841 caatattcag tggtgaatta atagccataa ttgctgcctc ccctgcgtaa taaggtttgc
    45901 ggtcgtgtcg acctgaaatc aaacctgttt agcaagggaa gcaaaggggg cggagtccag
    45961 tcagtagcag gaggggcgca tacgcagcct ccctcgccat gcggggattt aatcatcggc
    46021 atcatctcat ttcctcgtca cagtcttaat cgttaacgga agcccgttgc cgcctgcgct
    46081 ccataacgcg acatccggga aatgtctgga ttaccagcaa atgtcaggcc tggctccgct
    46141 caggccgatg aaacaacccc gcaaggggaa ttacgcgttt ttcaacatcc actcaatttc
    46201 tgtttctgtg atgaggcgct caaactgcaa cagctcatca tttttacagg cgtgatacac
    46261 atggcaaaac cgttcgccta aatagcggcg caggtgatca ttctcgataa actcacccag
    46321 ggcatcgctc tggcgaatcg ggaagggtaa gccttcctgt tccagcccgt tgccttcgac
    46381 ttcttcctgc aacggcagct cgttatcaag gccatgcaaa ataccggcaa aaatcgctgc
    46441 catcaccaga tatgggttgg catcggcacc cgccacgtga tattccacgc ggtgattatg
    46501 acggtcaccg cacggaatac gcagggcgac ggtgcggttg ttatggcccc acgacgcctg
    46561 cgtcggcaca tacattcccg gctggaagcg gcgatacgag ttcacgtttg gtgccagcaa
    46621 cgccatcgac gacggcatca ggtcaatcat cccggcgagc atctttttca gcagcggcga
    46681 atcttcgcct tctgtgtcag aaagcacatt ctcgccacga ttattttgca tactgatatg
    46741 gatatgcatc ccgctgcccg cgtgctcttc atacggcttc gccataaaag tggcgtgcat
    46801 cttatgcttt tctgccatca gacgcactag gcgttttagt gccagcgcat catcgcaggc
    46861 ttccagcacg ttatcagtat ggtaaaggtt gatttcaaac tgacccggcg aggcctcagc
    46921 gaccgcgcca tctgccggaa tcagctgcaa ctgcgccagt tcatcaatat cattgagcac
    46981 gtcggcgaag tggttgaggt tatcaacgga gtaaacctgg ctttgtgtat tgcggtcatc
    47041 ggtgccaggc gcgcagggcg gttgcagatc cccttcagcg tcgcgctggc gatccagtaa
    47101 atagaactcc agctctaccg ctacaaccgg gaacaatccg cgctggcgca gctgctgcca
    47161 caggcggtta agaacgttcc gcggctcaac gtcaaaggga gcgccatctt catcgaccat
    47221 ggtcaggagc atctgaccga taaactctgg atcggcggca gaaggagtta aggaaccgag
    47281 aacaggaaca caggtacgat ccggctcgcc catttcctga cccagacctg cttcttctac
    47341 tacgttgccc agaatatcca ttgcaaatac cgaggccggg aaataacacc ctttctcgag
    47401 cttcttcagg cttgaaacag ggatacgttt tccacggaag caaccattca aatcagtaag
    47461 taaaacatca acatattgcg tattcgggta gcgctccagg tagcgtttca cttcctgcgt
    47521 aaaggtgcta ccccgcctct cttctgactg ctgaacaaag ttctctactt caacgatatt
    47581 ggtttccatg attcttcgcc tttggtttgt tttccgctcg ttatcaaagc gtaaaatata
    47641 atgaccatca ttcgaatctg tatgcaaacc aaatgtttgt caaatgttaa attgagtttg
    47701 caaaaatgaa aatccactgc tagattgaaa aaatattgaa cataaaggtc atttaaagcg
    47761 cagtaatggc gataatttag tccactttgt gagattgagc atggaaaata taatgaacaa
    47821 tccggttatc ggtgtcgtaa tgtgcaggaa caggcttaag ggtcatgcga cccagactct
    47881 gcaagaaaag tacctgaatg ccatcatcca cgcaggcggc ttgccaattg cgctaccaca
    47941 tgcgctggcg gaaccgtcgt tacttgaaca acttttgccg aaactcgatg gcatttatct
    48001 tcctagtagt cccagcaatg tgcagccgca cctatatggt gaaaacggcg atgagcctga
    48061 cgccgatccc gggcgtgatc ttctgagcat ggcgataatt aatgccgcac tcgaaaggcg
    48121 catccccatt ttcgccatct gccggggttt acaagaactg gtggtggcaa ccggtggttc
    48181 gttgcatcgc aagctgtgcg aacagcctga attgctggaa catcgggaag atcccgaact
    48241 gccggtggaa cagcaatatg caccgtcgca tgaagttcag gttgaagagg ggggattact
    48301 gtctgcgttg ttacctgaat gtagcaactt ttgggtaaac tctctacatg gacaaggggc
    48361 gaaggtcgtt agcccacggt tgcgtgttga agctcgctcg ccggatggct tagttgaggc
    48421 ggttagcgtc atcaatcatc cttttgcgct gggcgtacag tggcacccgg aatggaacag
    48481 tagcgagtac gcgctttcgc gtatattgtt cgagggcttt atcaccgctt ggcagcacca
    48541 tatcgctgaa aaacagcgac tctgaccact acagtttaag gaaatgcaaa tatgagtgat
    48601 gagggactgg cgccaggaaa acgcttgtcg gaaatccgcc agcagcaggg gctttcacaa
    48661 cgtcgtgccg ccgaactctc cgggctgact cacagtgcta tcagtacgat agaacaagat
    48721 aaagtcagcc ctgccatcag tacgctgcaa aagctgctga aggtgtatgg tttgtcactc
    48781 tcggaattct tttccgagcc ggaaaaacct gatgagccgc aggtcgtcat taatcaggac
    48841 gacttaattg agatgggcag tcagggtgtg tcaatgaagc tggttcataa cggtaacccg
    48901 aatcgcacgc tggcgatgat ctttgaaacg taccagccgg gcacaaccac tggggaaaga
    48961 attaagcatc agggtgagga aataggcaca gtactggaag gtgaaattgt tctgacgatt
    49021 aatggtcagg attaccacct cgtcgcgggg caaagttatg ccattaatac cggcatcccg
    49081 cacagtttca gtaatacgtc ggcaggtatt tgccgaatta tcagcgccca tacgcccacc
    49141 acgttttaat ctttttgttc tgtaagccgg gtaagcgcaa tgcgcccggc aatccataaa
    49201 gaatgtcgcc agaaataaac gggcatgcag cccggggatc tctgcgccct gacgttcaca
    49261 aactgcatat atctgataga cgtgaaacag gagtcataat gaattttcat catctggctt
    49321 actggcagga taaagcgtta agtctcgcca ttgaaaaccg cttatttatt aacggtgaat
    49381 atactgctgc ggcggaaaat gaaacctttg aaaccgttga tccggtcacc caggcaccgc
    49441 tggcgaaaat tgcccgcggc aagagcgtcg atatcgaccg tgcggtgagc gcagcacgcg
    49501 gcgtatttga acgcggcgac tggtcactct cttctccggc taaacgtaaa gcggtactga
    49561 ataaactcgc cgatttaatg gaagcccacg ccgaagagct ggcactgctg gaaactctcg
    49621 acaccggcaa accgattcgt cacagtctgc gtgatgatat tcccggcgcg gcgcgcgcca
    49681 ttcgctggta cgccgaagcg atcgacaaag tgtatggcga agtggcgacc accagtagcc
    49741 atgagctggc gatgatcgtg cgtgaaccgg tcggcgtgat tgccgccatc gtgccgtgga
    49801 acttcccgct gttgctgact tgctggaaac tcggcccggc gctggcggcg ggaaacagcg
    49861 tgattctaaa accgtctgaa aaatcaccgc tcagtgcgat tcgtctcgcg gggctggcga
    49921 aagaagcagg cttgccggat ggtgtgttga acgtggtgac gggttttggt catgaagcgg
    49981 ggcaggcgct ttcgcgtcat aacgatatcg atgccatcgc ctttaccggc tccacgcgta
    50041 cgggtaaaca actgctgaaa gacgcgggtg atagcaacat gaaacgtgtc tggctggaag
    50101 cgggcggcaa aagcgccaac atcgttttcg ccgattgtcc ggatctgcaa aaagcggcaa
    50161 gcgccaccgc cgcaggcatc ttctacaacc agggccaggt gtgcatcgcc ggaacgcgtt
    50221 tgttactgga agagagcatc gccgatgaat tcttagccct gttaaaacag caggcgcaaa
    50281 actggcagcc gggccatcca cttgatcccg caaccaccat gggcacctta atcgacagcg
    50341 ctcacgctga ctcggtacac acctttattc gtgaaggcga aggcaagggg caactgctgc
    50401 tggatggtcg caacgccggg ctggcagcgg ccatcggccc gaccatcttc gtggatgtgg
    50461 acccgaatgc gtccttaagc cgcgaagaga ttttcggtcc ggtgctggtg gtcacccgtt
    50521 tcacatcaga agatcaggcg ctgcagcttg ccaacgacag ccagtacggc cttggcgcgg
    50581 cggtatggac gcgcgacctc tcccgcgcgc accgcatgag ccgacgcctg aaagccggtt
    50641 ccgtcttcgt caataactac aacgacggcg atatgaccgt gccgtttggc ggctataagc
    50701 agagcggcaa cggtcgcgac aaatccctgc atgcccttga aaaattcact gaactgaaaa
    50761 ccatctggat aagcctggag gcctgaaatg accgaacata ccagcagtta ctacgccgcc
    50821 agtgcgaata aatatgcacc attcgacaca ctgaatgagt caatcacctg cgacgtttgc
    50881 gtggttggcg gcggctatac cgggctctcc tccgcgctgc atctggcgga agcgggcttt
    50941 gacgtagtgg ttctcgaagc ctcacgcatc ggctttggcg caagcgggcg caatggcggg
    51001 cagcttgtga actcctacag ccgcgacatc gacgtgatcg aaaaaagcta cggcatggac
    51061 accgcccgta tgctcggcag catgatgttc gagggtggcg agatcatccg cgaacgtatc
    51121 aaacgttatc agattgactg cgactaccgc cccggcggtc tgtttgtggc gatgaatgat
    51181 aaacaactcg ccacactcga agagcagaaa gagaactggg aacgctacgg caataaacag
    51241 ctggagctgc tggacgtcaa cgctattcgc cgcgaggtcg ccagcgatcg ctacactggt
    51301 gcgctgctgg atcacagcgg tgggcatatt cacccgctaa acctcgccat tggtgaagcc
    51361 gatgccatcc gcctgaacgg cgggcgcgtg tatgagcttt ccgccgtgac gcagatccag
    51421 cacaccacgc cagccgtggt tcggactgcc aatggtcagg tgacggcgaa gtatgtgatt
    51481 gtcgccggga atgcgtatct gggcgataaa gtagagccgg aactagcgaa acgcagcatg
    51541 ccgtgcggca cgcaggtgat caccaccgaa cggctgtcgg aagatttagc ccgttcgctg
    51601 atcccgaaaa actactgtgt ggaagactgt aactatctgc ttgattacta ccgtcttacc
    51661 gccgacaacc gcctgctgta cggcgggggt gtagtgtatg gtgcacgcaa cccggatgac
    51721 gttgagcgtc ttgtggtacc gaaactacta aaaaccttcc ctcagttgaa gggcgtgaaa
    51781 attgattacc gctggacggg caacttcctg ctgaccttgt cgcacatgcc gcagttcggc
    51841 cgccttgata ccaacatcta ttacatgcag ggttacagcg gccacggcgt cacctgtacc
    51901 catcttgccg ggcgtttgat tgctgaactg ctgcgcggtg acgccgagcg tttcgatgcc
    51961 ttcgccaatc tgccgcatta cccgttcccc ggcgggcgca cgctgcgcgt gccatttacc
    52021 gcgatgggcg cggcttatta cagcctgcgc gatcgtctgg gcgtttaatt tccgattaac
    52081 agtgaagagt caaaaggtgt gaaacatgag caacaatgaa ttccatcagc gtcgtctttc
    52141 tgctacccca cgcggggttg gcgtgatgtg taacttcttc gcccagtcgg cagaaaacgc
    52201 tacgctgaag gacgtagagg gcaacgaata catcgatttc gccgcaggca ttgcggtgct
    52261 gaataccggg catcgccacc ctgatctggt cgcggcggtg gagcagcaat tgcaacagtt
    52321 tacccacacg gcgtatcaga ttgtgccgta cgaaagctac gtcacgctgg cggagaaaat
    52381 taacaccctt gccccagtga gcgggcaggc taaaaccgca ttcttcacta ccggcgcgga
    52441 agcggtggaa aacgcggtga aaattgctcg cgcccatacc ggacgacctg gcgtgattgc
    52501 gtttagcggt ggcttccacg gtcgtacata tatgaccatg gcgctgaccg gaaaagttgc
    52561 gccgtacaaa atcggcttcg gcccgttccc tggttcggtg tatcacgtac cttatccgtc
    52621 agatttacac ggcatttcaa cacaggactc cctcgacgcc atcgaacgct tgtttaaatc
    52681 agacatcgaa gcgaagcagg tcgcggccat tattttcgaa ccggtgcagg gcgagtgcgg
    52741 tttcaacgtt gcgccaaaag agctggttgc cgccattcgc cgcctgtgcg acgagcacgg
    52801 tattgtgatg attgccgatg aagtgcaaag cggctttgcg cgtaccggta aactgtttgc
    52861 catggatcat tacgccgata agccggactt aatgacgatg gcgaaaagcc tcgcgggcgg
    52921 gatgccgctt tcgggcgtgg tcggtaacgc gaatattatg gacgcgcccg cgccgggcgg
    52981 gttgggtggc acctacgccg ggaacccgct ggcggtggct gccgcgcacg cggtgctcaa
    53041 tattatcgac aaagaatcac tctgcgaacg cgcgaatcaa ctgggccagc gtctcaaaaa
    53101 cacgttgatt gatgccaaag aaagcgttcc ggccattgct gcggtacgcg gcctggggtc
    53161 gatgattgcg gcagagttta acgatccgca aacgggcgag ccgtcagcgg cgattgcaca
    53221 gaaaatccag caacgcgcgc tggcgcaggg gctgctcctg ctgacctgtg gcgcatacgg
    53281 caacgtgatt cgcttcctgt atccgctgac catcccggat gcgcaattcg atgcggcaat
    53341 gaaaattttg caggatgcgc tgagcgatta atagcccaac gcaataatgt ctgatgcgct
    53401 gcgcttatca ggcctgcaaa cgacgtattg attatgtatg ccgaataaag cattcacgcc
    53461 gcatccggca agttgtattg ctcaacttcg ctaaatctgg tgctttttca acaacgcgcg
    53521 gaactgatga taagttaacc ccagtaattc agccgcgcgc ttctggttat atttcccctg
    53581 ttgcaaactg agttgcagca actctttttc ctgctgcatc tgaaactcgc gtaaatccag
    53641 cggcagcgtc ggaagcgagg tattttctga aacggcgatg gcttcttcag gtggacgccg
    53701 tttaaaggga tcaataatga tgtcatcaag cggataatcg ctggtgccgt ggcgatacac
    53761 tgaacgttcc accacgtttt tcaattcacg aatatttccc ggccaacgat aattcagcaa
    53821 tgtttctctg gcgcgctccg taaaccccgg gaacagaggc agcttgattt cccgacacat
    53881 ctgaatggca aagtgttctg ccatcagcat aatgtcgctt tcgcgctcgc gcaggggcgg
    53941 cagttgcact acatcgaaag ccagccggtc gagcaggtca gcgcgaaaag taccttcatt
    54001 gaccatcgcc gggagatcgg cattcgtcgc gcataccaac cgcacattca cctgcaacgg
    54061 ctggctaccg ccaacgcgct ccagttcacc gtactcaatc acacgcaata atttttcctg
    54121 caccatcatc ggtgccgtag cgagttcatc aagaaatagc gtaccgccgt cggcacgttc
    54181 aaatctccca ggatgacgtt tttgcgcacc ggtaaacgcc cccgcttcgt gaccaaacag
    54241 ttcggaatcc agcagatttt catttaacgc cgcgcagtta agggaaataa acggcccttg
    54301 ccaacgggag gagagataat gcaggcggct ggcaatcaac tctttaccgg tgccgcgttc
    54361 gccgatgatg agcaccggtt tgtccagcgg tgcgagatgc gaaacctgtt ccagcacttc
    54421 gagaaagctg ttcgcctcac caagtaaatt atctttgtat tcggccatga tgaaattcgc
    54481 cacttgttag tgtaattcgc taattcatcc tggcatgttg ctgttgattc ttcaatcaga
    54541 tctttataaa tcaaaaagat aaaaaattgg cacgcaaatt gtattaacag ttcagcagga
    54601 caatcctgaa cgcagaaatc aagaggacaa cattatgggt attttttctc gctttgccga
    54661 catcgtgaat gccaacatca acgctctgtt agagaaagcg gaagatccac agaaactggt
    54721 gcgtctgatg atccaggaga tggaagatac actggttgaa gtacgttcta cttcggcgcg
    54781 tgcgttggca gaaaagaaac agctgactcg ccgtattgaa caagcgtcgg cgcgtgaagt
    54841 tgaatggcag gaaaaagccg aactggcgct gctgaaagag agagaggatc tggcacgtgc
    54901 agcgttaatt gaaaaacaga aactgaccga tctgattaag tccctggaac atgaagtgac
    54961 gctggtggac gatacgctgg cacgcatgaa gaaagagatt ggcgagctgg aaaacaaatt
    55021 gagcgaaaca cgcgctcgcc agcaggcatt gatgttacgt catcaggcgg caaactcgtc
    55081 gcgcgatgtg cgtcgtcagc tggacagtgg caaactggat gaagcaatgg ctcgtttcga
    55141 atctttcgaa cgtcgtattg accagatgga agcggaagca gaaagccaca gcttcggtaa
    55201 acaaaaatcg ctggacgatc agtttgccga actgaaagcc gatgatgcaa tcagcgaaca
    55261 actggcacaa ttaaaagcca aaatgaagca agacaatcaa taataacatc caggcggcgt
    55321 ccgaacgcgc cgccgctcat cgtctaagga gtacttatga gcgcgctatt tctggctatt
    55381 ccgttaacca tttttgtgct gtttgtttta ccgatctggt tatggctgca ttacagcaat
    55441 cgttctggtc gcagtgaatt gtcgcaaagt gagcagcagc gattagcgca actggttgat
    55501 gaagcaaaac ggatgcgcga acgtattcag gcgctggaat ctattcttga tgcagaacat
    55561 ccgaactgga gggatcgcta atggcgggca ttaatctcaa taaaaagtta tggcgtattc
    55621 cacagcaggg catggtccgc ggcgtctgcg ccgggattgc caactatttt gatgtaccgg
    55681 taaaactggt gcgtatcctg gtggtgctgt cgattttctt cggtctggcg ctgtttaccc
    55741 tggttgctta catcattttg tcatttgcgc ttgatccaat gccggacaac atggcctttg
    55801 gtgagcagct accttccagc agcgaattgc tggatgaagt cgaccgtgaa ctggcggcaa
    55861 gtgaaacgcg tttacgcgag atggaacgtt atgtcacttc cgatactttc acgttacgta
    55921 gccgtttccg tcaactgtga ggaaagttat gaatactcgc tggcaacagg ccgggcaaaa
    55981 ggtgaagctt ggtttcaaat tagcaggcaa gctggtactt cttaccgcac tgcgctatgg
    56041 cccggcgggt gtggcgggct gggcgataaa atcagttgct cgccgaccgc tgaaaatgtt
    56101 gctggctgtg gcgctggaac cgctgttaag tcgggctgct aataaactgg cacagcgtta
    56161 taaaaggtga ggggagtttc gcaaaaattg ttaaatctca ggcgtataat ggatggcaat
    56221 tttcatccat agaaggacgc ttacatgttt aaaaaaaggc ttacttgctc tggcactggt
    56281 gttttcactg cccgttttcg ccgctgaaca ctggatcgat gttcgtgttc cagagcagta
    56341 tcagcaagag cacgttcagg gggccatcaa tattcccctg aaggaagtga aagagcgcat
    56401 tgccaccgcc gttccggata aaaacgacac cgtgaaa
//
LOCUS       DBKELA010000014        53200 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.014, whole genome shotgun sequence.
ACCESSION   DBKELA010000014 DBKELA010000000
VERSION     DBKELA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.014"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(11..322)
                     /locus_tag="ACUQR0_000830"
     CDS             complement(11..322)
                     /locus_tag="ACUQR0_000830"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HHV9107990.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACUQR0_000831"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACUQR0_000831"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACUQR0_000832"
     CDS             701..1054
                     /locus_tag="ACUQR0_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HHV9107991.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACUQR0_000833"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACUQR0_000833"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HHV9107992.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACUQR0_000834"
     CDS             complement(2810..3280)
                     /locus_tag="ACUQR0_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HHV9107993.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACUQR0_000835"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACUQR0_000835"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HHV9107994.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /locus_tag="ACUQR0_000836"
     CDS             complement(4119..4337)
                     /locus_tag="ACUQR0_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HHV9107995.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACUQR0_000837"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACUQR0_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HHV9107996.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACUQR0_000838"
     CDS             complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACUQR0_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HHV9107997.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACUQR0_000839"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACUQR0_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HHV9107998.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="ACUQR0_000840"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="ACUQR0_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HHV9107999.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="ACUQR0_000841"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="ACUQR0_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HHV9108000.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACUQR0_000842"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACUQR0_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HHV9108001.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACUQR0_000843"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACUQR0_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HHV9108002.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="ACUQR0_000844"
     CDS             14911..15288
                     /locus_tag="ACUQR0_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HHV9108003.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="ACUQR0_000845"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="ACUQR0_000845"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HHV9108004.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="ACUQR0_000846"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="ACUQR0_000846"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HHV9108005.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="ACUQR0_000846"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="ACUQR0_000847"
     CDS             18099..18428
                     /locus_tag="ACUQR0_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HHV9108006.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="ACUQR0_000848"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="ACUQR0_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HHV9108007.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="ACUQR0_000849"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="ACUQR0_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HHV9108008.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="ACUQR0_000850"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="ACUQR0_000850"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HHV9108009.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="ACUQR0_000851"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="ACUQR0_000851"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HHV9108010.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACUQR0_000852"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACUQR0_000852"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HHV9108011.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="ACUQR0_000853"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="ACUQR0_000853"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HHV9108012.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACUQR0_000854"
     CDS             complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACUQR0_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HHV9108013.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACUQR0_000855"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACUQR0_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HHV9108014.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="ACUQR0_000856"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="ACUQR0_000856"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HHV9108015.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACUQR0_000857"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACUQR0_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HHV9108016.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="ACUQR0_000858"
     CDS             complement(31205..31999)
                     /locus_tag="ACUQR0_000858"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HHV9108017.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="ACUQR0_000859"
     CDS             32137..32478
                     /locus_tag="ACUQR0_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HHV9108018.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACUQR0_000860"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACUQR0_000860"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HHV9108019.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="ACUQR0_000861"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="ACUQR0_000861"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HHV9108020.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="ACUQR0_000862"
     CDS             36394..37686
                     /locus_tag="ACUQR0_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HHV9108021.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37717..37814
                     /locus_tag="ACUQR0_000863"
                     /pseudo
     CDS             37717..37814
                     /locus_tag="ACUQR0_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="ACUQR0_000864"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="ACUQR0_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HHV9108022.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="ACUQR0_000865"
     CDS             complement(38219..38677)
                     /locus_tag="ACUQR0_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HHV9108023.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="ACUQR0_000866"
     CDS             complement(38674..39591)
                     /locus_tag="ACUQR0_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HHV9108024.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="ACUQR0_000867"
                     /gene_synonym="ybbL"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="ACUQR0_000867"
                     /gene_synonym="ybbL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:1903988 - iron ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron efflux ABC transporter ATP-binding subunit
                     FetA"
                     /protein_id="HHV9108025.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="ACUQR0_000868"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="ACUQR0_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:1903988 - iron ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron efflux ABC transporter permease subunit
                     FetB"
                     /protein_id="HHV9108026.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACUQR0_000869"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACUQR0_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HHV9108027.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACUQR0_000870"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACUQR0_000870"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HHV9108028.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACUQR0_000871"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACUQR0_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /gene="ybbA"
                     /locus_tag="ACUQR0_000872"
     CDS             43550..44236
                     /gene="ybbA"
                     /locus_tag="ACUQR0_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HHV9108029.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /gene="ybbP"
                     /locus_tag="ACUQR0_000873"
                     /pseudo
     CDS             44233..46648
                     /gene="ybbP"
                     /locus_tag="ACUQR0_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACUQR0_000874"
     CDS             complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACUQR0_000874"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HHV9108030.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACUQR0_000875"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACUQR0_000875"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HHV9108031.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACUQR0_000876"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACUQR0_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HHV9108032.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="ACUQR0_000877"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="ACUQR0_000877"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HHV9108033.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="ACUQR0_000878"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="ACUQR0_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HHV9108034.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="ACUQR0_000879"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="ACUQR0_000879"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HHV9108035.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53200
                     /locus_tag="ACUQR0_000880"
     CDS             52938..>53200
                     /locus_tag="ACUQR0_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HHV9108036.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVA"
BASE COUNT        12826 a        13688 c        14018 g        12668 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc
//
LOCUS       DBKELA010000015        52077 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.015, whole genome shotgun sequence.
ACCESSION   DBKELA010000015 DBKELA010000000
VERSION     DBKELA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.015"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="ACUQR0_000881"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="ACUQR0_000881"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HHV9108037.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="ACUQR0_000882"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="ACUQR0_000882"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HHV9108038.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="ACUQR0_000883"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="ACUQR0_000883"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHV9108039.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="ACUQR0_000884"
     CDS             complement(5906..6949)
                     /locus_tag="ACUQR0_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HHV9108040.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="ACUQR0_000885"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="ACUQR0_000885"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HHV9108041.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="ACUQR0_000886"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="ACUQR0_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HHV9108042.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="ACUQR0_000887"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="ACUQR0_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="ACUQR0_000888"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="ACUQR0_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HHV9108043.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="ACUQR0_000889"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="ACUQR0_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HHV9108044.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="ACUQR0_000890"
     CDS             15474..15830
                     /locus_tag="ACUQR0_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HHV9108045.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="ACUQR0_000891"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="ACUQR0_000891"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HHV9108046.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="ACUQR0_000892"
     CDS             16989..17189
                     /locus_tag="ACUQR0_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HHV9108047.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="ACUQR0_000893"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="ACUQR0_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HHV9108048.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="ACUQR0_000894"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="ACUQR0_000894"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HHV9108049.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /gene="ypfJ"
                     /locus_tag="ACUQR0_000895"
     CDS             complement(20101..20964)
                     /gene="ypfJ"
                     /locus_tag="ACUQR0_000895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HHV9108050.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="ACUQR0_000896"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="ACUQR0_000896"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HHV9108051.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="ACUQR0_000897"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="ACUQR0_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HHV9108052.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDVGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="ACUQR0_000898"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="ACUQR0_000898"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HHV9108053.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="ACUQR0_000899"
     CDS             24133..24705
                     /locus_tag="ACUQR0_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HHV9108054.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="ACUQR0_000900"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="ACUQR0_000900"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HHV9108055.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="ACUQR0_000901"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="ACUQR0_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HHV9108056.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="ACUQR0_000902"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="ACUQR0_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="ACUQR0_000903"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="ACUQR0_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HHV9108057.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="ACUQR0_000904"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="ACUQR0_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HHV9108058.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="ACUQR0_000905"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="ACUQR0_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HHV9108059.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="ACUQR0_000906"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="ACUQR0_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HHV9108060.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="ACUQR0_000907"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="ACUQR0_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="ACUQR0_000908"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="ACUQR0_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HHV9108061.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="ACUQR0_000909"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="ACUQR0_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HHV9108062.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="ACUQR0_000910"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="ACUQR0_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HHV9108063.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="ACUQR0_000911"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="ACUQR0_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HHV9108064.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /gene="focB"
                     /locus_tag="ACUQR0_000912"
                     /pseudo
     CDS             38213..39061
                     /gene="focB"
                     /locus_tag="ACUQR0_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(39099..40160)
                     /locus_tag="ACUQR0_000913"
     CDS             complement(39099..40160)
                     /locus_tag="ACUQR0_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHV9108065.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="ACUQR0_000914"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="ACUQR0_000914"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HHV9108066.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="ACUQR0_000915"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="ACUQR0_000915"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HHV9108067.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43050)
                     /locus_tag="ACUQR0_000916"
     CDS             complement(42349..43050)
                     /locus_tag="ACUQR0_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HHV9108068.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="ACUQR0_000917"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="ACUQR0_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HHV9108069.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="ACUQR0_000918"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="ACUQR0_000918"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HHV9108070.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="ACUQR0_000919"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="ACUQR0_000919"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HHV9108071.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="ACUQR0_000920"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="ACUQR0_000920"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HHV9108072.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="ACUQR0_000921"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="ACUQR0_000921"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HHV9108073.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="ACUQR0_000922"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="ACUQR0_000922"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HHV9108074.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLVPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52077)
                     /gene="pdeF"
                     /locus_tag="ACUQR0_000923"
     CDS             complement(50969..>52077)
                     /gene="pdeF"
                     /locus_tag="ACUQR0_000923"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HHV9108075.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12157 a        13738 c        13520 g        12662 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 caacatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggtgccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgt
//
LOCUS       DBKELA010000016        49162 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.016, whole genome shotgun sequence.
ACCESSION   DBKELA010000016 DBKELA010000000
VERSION     DBKELA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.016"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1151)
                     /gene="setB"
                     /locus_tag="ACUQR0_000924"
     CDS             complement(<1..1151)
                     /gene="setB"
                     /locus_tag="ACUQR0_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HHV9108076.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVLLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1519..2649
                     /gene="fruB"
                     /locus_tag="ACUQR0_000925"
     CDS             1519..2649
                     /gene="fruB"
                     /locus_tag="ACUQR0_000925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HHV9108077.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2649..3587
                     /gene="fruK"
                     /locus_tag="ACUQR0_000926"
     CDS             2649..3587
                     /gene="fruK"
                     /locus_tag="ACUQR0_000926"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HHV9108078.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3604..5295
                     /gene="fruA"
                     /locus_tag="ACUQR0_000927"
     CDS             3604..5295
                     /gene="fruA"
                     /locus_tag="ACUQR0_000927"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HHV9108079.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5719..6660
                     /locus_tag="ACUQR0_000928"
     CDS             5719..6660
                     /locus_tag="ACUQR0_000928"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HHV9108080.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6648..7586
                     /gene="psuG"
                     /locus_tag="ACUQR0_000929"
     CDS             6648..7586
                     /gene="psuG"
                     /locus_tag="ACUQR0_000929"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HHV9108081.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7680..8930
                     /locus_tag="ACUQR0_000930"
     CDS             7680..8930
                     /locus_tag="ACUQR0_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HHV9108082.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9001..9695)
                     /locus_tag="ACUQR0_000931"
                     /pseudo
     CDS             complement(9001..9695)
                     /locus_tag="ACUQR0_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9826..10767
                     /gene="rihB"
                     /locus_tag="ACUQR0_000932"
                     /pseudo
     CDS             9826..10767
                     /gene="rihB"
                     /locus_tag="ACUQR0_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10867..12117
                     /locus_tag="ACUQR0_000933"
     CDS             10867..12117
                     /locus_tag="ACUQR0_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HHV9108083.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12173..13261)
                     /locus_tag="ACUQR0_000934"
     CDS             complement(12173..13261)
                     /locus_tag="ACUQR0_000934"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HHV9108084.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13264..14121)
                     /gene="nfo"
                     /locus_tag="ACUQR0_000935"
     CDS             complement(13264..14121)
                     /gene="nfo"
                     /locus_tag="ACUQR0_000935"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HHV9108085.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14195..15244)
                     /locus_tag="ACUQR0_000936"
     CDS             complement(14195..15244)
                     /locus_tag="ACUQR0_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HHV9108086.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15343..16224
                     /gene="yieE"
                     /locus_tag="ACUQR0_000937"
     CDS             15343..16224
                     /gene="yieE"
                     /locus_tag="ACUQR0_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HHV9108087.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16430..17899
                     /gene="lysP"
                     /locus_tag="ACUQR0_000938"
     CDS             16430..17899
                     /gene="lysP"
                     /locus_tag="ACUQR0_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HHV9108088.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18191..20182
                     /gene="cirA"
                     /locus_tag="ACUQR0_000939"
     CDS             18191..20182
                     /gene="cirA"
                     /locus_tag="ACUQR0_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HHV9108089.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20214..21049)
                     /gene="yeiG"
                     /locus_tag="ACUQR0_000940"
                     /pseudo
     CDS             complement(20214..21049)
                     /gene="yeiG"
                     /locus_tag="ACUQR0_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21307..21975
                     /gene="folE"
                     /locus_tag="ACUQR0_000941"
     CDS             21307..21975
                     /gene="folE"
                     /locus_tag="ACUQR0_000941"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HHV9108090.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21992..23068
                     /gene="yeiB"
                     /locus_tag="ACUQR0_000942"
     CDS             21992..23068
                     /gene="yeiB"
                     /locus_tag="ACUQR0_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HHV9108091.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23210..24250
                     /gene="galS"
                     /locus_tag="ACUQR0_000943"
     CDS             23210..24250
                     /gene="galS"
                     /locus_tag="ACUQR0_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HHV9108092.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24530..25528
                     /gene="mglB"
                     /locus_tag="ACUQR0_000944"
     CDS             24530..25528
                     /gene="mglB"
                     /locus_tag="ACUQR0_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HHV9108093.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25589..27109
                     /gene="mglA"
                     /locus_tag="ACUQR0_000945"
     CDS             25589..27109
                     /gene="mglA"
                     /locus_tag="ACUQR0_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HHV9108094.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27125..28135
                     /gene="mglC"
                     /locus_tag="ACUQR0_000946"
     CDS             27125..28135
                     /gene="mglC"
                     /locus_tag="ACUQR0_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HHV9108095.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28171..29384)
                     /gene="preA"
                     /locus_tag="ACUQR0_000947"
                     /pseudo
     CDS             complement(28171..29384)
                     /gene="preA"
                     /locus_tag="ACUQR0_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29378..30616)
                     /gene="preT"
                     /locus_tag="ACUQR0_000948"
     CDS             complement(29378..30616)
                     /gene="preT"
                     /locus_tag="ACUQR0_000948"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HHV9108096.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30810..31049)
                     /locus_tag="ACUQR0_000949"
     CDS             complement(30810..31049)
                     /locus_tag="ACUQR0_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HHV9108097.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="ACUQR0_000950"
     CDS             complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="ACUQR0_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HHV9108098.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="ACUQR0_000951"
     CDS             complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="ACUQR0_000951"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HHV9108099.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32935..33630)
                     /locus_tag="ACUQR0_000952"
     CDS             complement(32935..33630)
                     /locus_tag="ACUQR0_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HHV9108100.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33627..34025)
                     /locus_tag="ACUQR0_000953"
     CDS             complement(33627..34025)
                     /locus_tag="ACUQR0_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HHV9108101.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34264..35211
                     /gene="dusC"
                     /locus_tag="ACUQR0_000954"
     CDS             34264..35211
                     /gene="dusC"
                     /locus_tag="ACUQR0_000954"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HHV9108102.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="ACUQR0_000955"
     CDS             complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="ACUQR0_000955"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HHV9108103.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35889..37325
                     /gene="mdtQ"
                     /locus_tag="ACUQR0_000956"
     CDS             35889..37325
                     /gene="mdtQ"
                     /locus_tag="ACUQR0_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HHV9108104.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37417..38139
                     /locus_tag="ACUQR0_000957"
                     /pseudo
     CDS             37417..38139
                     /locus_tag="ACUQR0_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38256..38834)
                     /locus_tag="ACUQR0_000958"
     CDS             complement(38256..38834)
                     /locus_tag="ACUQR0_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9108105.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39004..39591
                     /locus_tag="ACUQR0_000959"
     CDS             39004..39591
                     /locus_tag="ACUQR0_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HHV9108106.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39765..40697
                     /gene="pbpG"
                     /locus_tag="ACUQR0_000960"
     CDS             39765..40697
                     /gene="pbpG"
                     /locus_tag="ACUQR0_000960"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HHV9108107.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40735..42450)
                     /gene="dld"
                     /locus_tag="ACUQR0_000961"
     CDS             complement(40735..42450)
                     /gene="dld"
                     /locus_tag="ACUQR0_000961"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HHV9108108.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42646..44943
                     /gene="bglX"
                     /locus_tag="ACUQR0_000962"
     CDS             42646..44943
                     /gene="bglX"
                     /locus_tag="ACUQR0_000962"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HHV9108109.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45195..46112
                     /gene="osmF"
                     /locus_tag="ACUQR0_000963"
     CDS             45195..46112
                     /gene="osmF"
                     /locus_tag="ACUQR0_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HHV9108110.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46119..47276
                     /gene="yehY"
                     /locus_tag="ACUQR0_000964"
     CDS             46119..47276
                     /gene="yehY"
                     /locus_tag="ACUQR0_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HHV9108111.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47269..48195
                     /gene="yehX"
                     /locus_tag="ACUQR0_000965"
     CDS             47269..48195
                     /gene="yehX"
                     /locus_tag="ACUQR0_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HHV9108112.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48200..48931
                     /gene="yehW"
                     /locus_tag="ACUQR0_000966"
     CDS             48200..48931
                     /gene="yehW"
                     /locus_tag="ACUQR0_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HHV9108113.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48912..49019)
                     /locus_tag="ACUQR0_000967"
     CDS             complement(48912..49019)
                     /locus_tag="ACUQR0_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HHV9108114.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49079..>49162)
                     /locus_tag="ACUQR0_000968"
     CDS             complement(49079..>49162)
                     /locus_tag="ACUQR0_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHV9108115.1"
                     /translation="GENRTSAQQQQLTSWQEQGHDIFPLGI"
BASE COUNT        11873 a        12104 c        13201 g        11984 t
ORIGIN      
        1 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
       61 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      121 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      181 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      241 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      301 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      361 agcccggcgg cggtccccat catcacaccg gctagtttct cgggaagatg cagttcgttg
      421 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      481 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      541 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      601 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      661 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      721 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      781 gggttagcgg tcgagccaaa gctgctaaga aggacgccaa cgaatagcaa aacaaagtag
      841 ttgcgattcc aagcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      901 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
      961 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1021 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1081 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1141 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1201 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1261 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1321 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1381 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1441 gtctttaacc ctaagctagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1501 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1561 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1621 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1681 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1741 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1801 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1861 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1921 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     1981 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2041 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2101 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2161 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2221 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2281 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2341 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt agtgcgcaat
     2401 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2461 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2521 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2581 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2641 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2701 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2761 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2821 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2881 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     2941 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt cgaagtcacc cccgccgact
     3001 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3061 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3121 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3181 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3241 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3301 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3361 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3421 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3481 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3541 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3601 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3661 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3721 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3781 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3841 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3901 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     3961 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4021 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4081 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4141 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4201 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4261 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4321 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4381 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4441 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4501 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4561 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4621 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4681 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4741 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4801 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4861 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4921 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     4981 caggaaggtg gcaaagccgc tctggtattg ggactgtgct tcatttcgga aggtgcaatt
     5041 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5101 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5161 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5221 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5281 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5341 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5401 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5461 tattttctgc ccttttttag tgttcctgtt ctgtgcatcc cgtcacaaat tcctcgcttt
     5521 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5581 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5641 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5701 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5761 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5821 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     5881 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     5941 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6001 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6061 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6121 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6181 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgcggaaatg tgtcaaagtg
     6241 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6301 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6361 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6421 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6481 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6541 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6601 cccgatttat cgattgccaa cgttatatcg ttagtggaga acacagaatg tctgaattaa
     6661 aaatctcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6721 aaccggttgt ggcgctggaa tcgaccatta tttctcacgg gatgccgttc ccacaaaatg
     6781 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6841 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6901 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     6961 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7021 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7081 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7141 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7201 taattggcta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7261 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7321 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7381 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7441 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
     7501 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7561 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7621 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7681 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7741 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7801 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7861 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7921 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     7981 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8041 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8101 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8161 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8221 tggcgtccat tgctggttcg gtgatgattg gttatgccgg aatgggcgta ccaattgact
     8281 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8341 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8401 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8461 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8521 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8581 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8641 gcctgattgg gcagaaactg gcgattaacg aattcgtcgc ttacctgagt ttctccccat
     8701 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8761 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8821 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8881 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctcgattt
     8941 gtagaccgga taaaacgtat taagcgttgc atccggcaat cgcgctattg caaataattt
     9001 ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgcca gtcctgacaa
     9061 ctgctttctg tttttaatta gatatccttt cttttaatta ataaaccgtc gtgaatgaat
     9121 tgcgcgagaa catataaaag atgtcgataa gaaacgccta aatactctgc cgcttgcgta
     9181 tgcttttcgt gataaagatc accttcttgt gagagtaata taaatgctgc caggcgattc
     9241 actaacggaa atgattgatt ctgagttaaa gaaacaatat tacgataatt tttatgactt
     9301 aaggtgacgc aaagttttcg taaaaatagg gtgtcgttta ataacagcgg acggtaatgt
     9361 ttcataggga gcgcaaggca ccaacactct tcaatagcct gcaccgcacg cggttcatgg
     9421 tctttgtcga ttaactcaat ctcgccaata aaacagggag cggcaaagaa atcgatcagc
     9481 gacacgcgac cattggcaag cgtggcgtaa agcctggcgc ggcctcgcgt cagataaaac
     9541 agccatgacg gctgttgccc ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc
     9601 cgcgtatcgg ctgaaacatc cgttgaaaag caatccttaa acgcagattc actcatcaat
     9661 tgctgcttaa ggtcattatt gtggatttct ttcacgatgt ttatccgcta tgagatttct
     9721 catatttagc atacgtacgc tgttgttaag attcaatcat tggcccggtc gaattttctt
     9781 tgctgtagaa aaaaataacg caactggaaa cagaggaaat aaacaatgga aaagagaaaa
     9841 attattctgg attgtgagcc gggtcatgat gatgctattg caatgatgat ggcagcgaaa
     9901 catccggcaa tagatttatt aggcatcact attgtagcgg gtaatcagac gcttgataaa
     9961 acattaatta atggcctgaa tgtttgccag aaactggaga ttaatgttcc ggtttatgcg
    10021 gggatgccgc aacccattat gcgtaaacaa atcgttgccg ataatattca cggtgaaacc
    10081 ggactggatg gcccggtatt cgagccgctg acccgctagg cagaaagtac ccatgcggtg
    10141 aaatatatca tcgataccct gatggcaagc gatggcgata tcactctggt gccggttggt
    10201 ccgctttcaa atatcgcggt ggcaatgcgt atgcaacccg caatcctgcc caaaatccgt
    10261 gaaattgtgc taatgggcgg cgcttacggt acaggcaact tcaccccatc tgccgagttc
    10321 aatatctttg ccgacccgga agccgcccgc gtggtgttca cctccggcgt tccattagtg
    10381 atgatgggcc tcgatcttac caaccagacc gtttgcaccc cggatgtaat tgctcggatg
    10441 gaaagggtag gcggccctgc cggagagctg ttcagcgaca tcatgaactt cactctcaaa
    10501 acgcagttcg aatactacgg cctggctggc ggcccggtgc atgacgccac ctgcattggt
    10561 tatctgatca accctgatgg cattaaaacc caggatatgt acgtcgaagt ggacgtcaac
    10621 agcggccctt gctacgggcg tacggtctgc gacgaactgg gcgttcttgg caagcccgct
    10681 aataccaaag tcggcatcac tattgatacc gactggttct ggggcttagt cgaagagtgc
    10741 gtgcgcggct acatcaaaac ccattaagtg ttgataacaa gccgggacag tatcgcaaga
    10801 tgcaggcccg gctatttctg atgggcagaa agccgtcgcc caaaatgaca tgaagagata
    10861 atcactatgg atgtcatgag aagtgttctg ggaatggtgg tactgctggc aattgcgttt
    10921 ttactgtcag taaacaagaa gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta
    10981 caggtcgtga ttggcgggat tatgctttgg ttaccgccag ggcgttgggt cgcagaaaaa
    11041 gtcgcttttg gcgtccacaa agtgatggcg tacagcgacg caggtagcgc atttatcttc
    11101 ggttctctgg tcggaccgaa aatggatacc ttatttgatg gcgcaggatt tatctttggt
    11161 ttcagggtat taccggcaat tatcttcgtc actgcactgg tgagtattct ctactacatc
    11221 ggtgtgatgg ggatcttaat tcgcattctc ggcggtatct tccagaaagc tttaaatatc
    11281 agcaagatcg agtcattcgt cgcggtcacc accattttcc tcgggcaaaa cgaaattccg
    11341 gcgatcgtca aaccctttat cgatcgtctg aatcgcaatg aattatttac agcgatttgt
    11401 agtggcatgg cctcgattgc tggttcgaca atgattggtt acgccgcact gggcgtacct
    11461 gtggaatatt tgctggcggc atcgttaatg gcgatccctg gcgggatctt gtttgcccgc
    11521 ctgttaagcc cggctacgga atcttcgcag gtttctttta ataacctctc tttcaccgaa
    11581 acaccgccaa aaagcattat tgaagccgat gcgacagggg caatgaccgg gctgaaaatc
    11641 gccgccggtg tagcgacagt tgttatggca tttgtcgcca tcattgcgtt aattaacggt
    11701 attatcggcg gcgttggcgg ctggtttggt tttgaacatg cctcgctgga gtccatttta
    11761 ggttacctgc tggccccact tgcgtgggta atgggtgttg actggagtga tgcaaatctt
    11821 gccgggagtt tgattggaca gaaactggca ataaatgaat ttgtcgctta tctcaatttc
    11881 tcaccctatc tgcaaacggc tggcactctg gatgctaaaa ccgtggcgat tatttccttc
    11941 gcgttgtgcg gtttcgctaa ctttggttct atcggggtgg tggtgggggc gttttctgcg
    12001 gttgcgccac accgtgcgcc ggaaatcgcc cagcttggtt tacgcgcgct ggcggcggcg
    12061 acactttcta atctgatgag tgccaccatt gccgggttct ttattggttt agcgtagtgt
    12121 gcaatttatg agccggatta gatgcggtca agcgtcgaat ccggcaacat tgttacacca
    12181 ttggcactaa tgacagcgcg ttatcggcag acagggtagg gttgtttaag ctgccgctgg
    12241 cgcgtgaaat tgccgcgcag gccatcgcaa aacgggcgct gtcacggaaa ttgtttccct
    12301 ccagaaagct atataccaga cccgccataa aaccatcgtc agcaccaaaa ctgtctaccg
    12361 tcgtatgcgc tggcacagtc agcaaaaatt gttcaccatc cttttcgctg caataaactg
    12421 actcatcggg caaataaaca aacagttgct gaacaccttg ctgatgcaat gcattcactg
    12481 cggcattacg gtcagcatcg ctggtgatcg cctgtcccca taaaatttcc agctccggta
    12541 aagtgggttt cagggtgtga atatgcgcca gccagtgttt gattttgccc gctttgaatt
    12601 ctgaaacggt atcgacaaac accgggatct catcagcgag ggtaaagacc cattccagcg
    12661 cctcggctgt caggttgcaa tcagccagta ccacgcccgc atgacgaagt aaatcgcgcg
    12721 acccgttcag tagttgcggt gtcaactgtt ccagcagatg ggtatcgtta attgccagca
    12781 cggtttgatc gtctcgattg gcaattgcca gatacgtcga tgtgctttga ccatgcaaac
    12841 gaacgcagcc ggagacattc acgccggcac ggcgcgtttc ttccaggagc atttcgccat
    12901 aaaagtcatc gccaatcact gaaagcaaat ggacgtcacg gcctaacagc gccagattgt
    12961 gggcgatatt gcgtccaacg ccgcctgccg aacaatgaat tgtaccggga tgagaagccg
    13021 cttgcgggta atggatatcc gccatcccgc gaatatccat attgattgtc cccaccacta
    13081 cgcagtattc ctgctcggtg agaatgtaac ctttgtcttt aatccggcct ttgcgcatta
    13141 aatccataat atgcgccgca acgcgcgaac gactgatttg cagcatgtcc gcaatttcgt
    13201 tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat ctccttttcc cggttattca
    13261 tcttcaggct accgcttttt cagtttgttg cgctttcagc caggcgatct cttctgccca
    13321 gatatccggg ttgatggttt caaggatcag cggaatgccg tcgaaacggt cgttctgcat
    13381 aatccagcgg aacgcatcat gaccgatatt gccttcaccg aggctatgat ggcggtcaac
    13441 gcggctgcca aaggtacttt tcgcatcgtt aaggtgcatc ccgcgcagat acttaaagcc
    13501 gacaatacgg gcaaaatcag cgaacgtttt ttcgcattcg gctggagtat gcaaatcata
    13561 cccggcagcg aaagcatggc aggtatcaat gcagacgccg acgcgggatt tatcttccac
    13621 gccgtcgata atcgccgcga gatgttcgaa tttaaaccct aagttactgc cctgaccggc
    13681 ggtgttttca atcaccgctg ttacaccttg agttttatcc agcgcaatgt tgatggattc
    13741 ggcaatacgc gcaaggcaat cctcttctga aatctgcatc agatggctgc cagggtggaa
    13801 gttgagcaaa gaaagcccca gttgttcgca acgctgcatt tcatctataa aggcatcgcg
    13861 cgatttttcc agagcttcag tgaccggatg tccgaggttg atcagataac tgtcgtgggg
    13921 aagaatttgc gccgatgtgt agtgatattt ttcacaggcg gctttgaatt catcgatggt
    13981 ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt ttggtgaaca aggcaaacgc
    14041 ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc agaccgccag cagcactaac
    14101 gtgcgctcca atgtatttca tgcgaggact cctgttaaac ccgctggagg aaaacggtaa
    14161 tgatagcggg ttaacaggag caagaagtag tggtttatgc gatgacgctt tgaatcacat
    14221 agttaatcgc accaccacca acaatcagcc aggcaaacag taccagtgcc atcagcagag
    14281 gtttcgcccc agcttttttc agcgcgctga cgtgagtggt cagacccagc gccgccatcg
    14341 ccattgccag caggaaggta tccagcgtta ccagcatgtt caccacgctc tgcggtaaca
    14401 ggtggaacga gttaaagatg gcaactacga tgaacaagat ggcaaaccac ggaatagtga
    14461 ttttgctttt ctcgccgctg ttcgccccag acagctgttt aacacgcgcc gccagcagga
    14521 tgaggaacgg agccagcatc atcacgcgca gcattttgga aataactgct gcgttttctg
    14581 catccgggct gatggcatga cctgccgcca ccacctgcgc cacttcgtgc acagtagaac
    14641 caatgtagat accgaaagtt tccggactaa accattgaga catcagcgga tatatcgccg
    14701 ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc acggttactt
    14761 tactggcttc cgctttcact accggctcag tcgccagtac cgcggcagca ccacagatac
    14821 tgctaccggc accgatcaac caactggtgt gcttatccag accaaacact ttctgcccca
    14881 ggaagcaagc cagcaggaag gtactggaca gcgtcagcac gtcaatgatg atcccactga
    14941 taccgacatc ggcaatttgc gagaacgtca gacggaagcc ataaagaatg atacccagac
    15001 gtaataaata ttgcttggca aacagcacac caccgtcaca gcttttccag atgtgcggat
    15061 agatggtgtt gcctaaaacc atccccaaca agattgcgag ggtgagggca ctaaacccgg
    15121 cacccgcaac cgcgggaatg gaaccacccc acagggcgac cccggtgata actgcactca
    15181 gggctaaccc cggaataaaa tgccacagtg tacgatgttg tttctgtaag gtgatattcg
    15241 tcataaccct ctcctttaac cgggcataag gttacgacta actggtttaa aaataaaatt
    15301 gattatatat ttataattaa tctttataag tggtaagcga ctatgcacat caccctccgg
    15361 cagttggaag tttttgcaga agtattgaaa agtggatcaa ccacccaggc gtcggtgatg
    15421 ctggcgttgt cgcaatcagc agtgagcgca gccttgactg acctggaagg gcagcttggc
    15481 gttcaactgt ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg gcggctgctc
    15541 tatccgcgag cgttggcact gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa
    15601 gacaacggcg cgattcgtat ctatgccagt agtaccatcg gtaactacat tctgcctgca
    15661 gttatcgccc gttatcgcca tgattatccg cagttgccga ttgaacttag cgttgggaac
    15721 agccaggacg tgatgcaagc ggtgctggat ttccgcgttg atattggctt tattgaagga
    15781 ccgtgccaca gcactgaaat catttctgaa ccgtggctgg aagacgagca ggtggttttc
    15841 gccgcgccga cttcgccgtt ggctcgtggt ccggtcacct tagaacagct ggccgctgcg
    15901 ccgtggatcc tgcgtgaacg cggttccggc acgcgggata ttgtcgatta tctgttgctg
    15961 tcacatttac cgaagtttga gatggcgatg gaattaggta actccgaggc aatcaaacat
    16021 gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac gtgtgattga agatcaattg
    16081 caggcaggca cattaagtga agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg
    16141 cgtatacatc atcggcaaaa acacctttcc aacgcgctac ggcgctttct ggactattgc
    16201 gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac atgctggtgc tgtgtcgatt
    16261 tcgtgacgca gcgccttcag catgcattcg ccagaaaaga gattggctgc tttacttata
    16321 atccctgggc gatcatgaag gtgtcttata accgtgtatt tctgccggaa ggattgccaa
    16381 tcgtctgcta caatcgcgcc atcattttta agatggatag catttttgta tgggttccga
    16441 aactaaaact acagaagcgc cgggcttacg ccgtgaatta aaggcgcgtc acctgacgat
    16501 gattgccatt ggcggttcca tcggtacagg tctttttgtt gcctctggcg caacgatttc
    16561 tcaggcaggt ccgggcgggg cattgctctc gtatatgctg attggcctga tggtttactt
    16621 cctgatgacc agtctcggtg aattggctgc atatatgccg gtttccggtt cgtttgccac
    16681 ttacggtcag aactatgttg aagaaggctt tggcttcgcg ctgggctgga actactggta
    16741 caactgggcg gtgactattg ccgttgacct ggttgcagca cagctggtca tgagctggtg
    16801 gttcccggat acaccgggct ggatctggag tgcgttgttc ctcggcgtta tcttcctgtt
    16861 gaactacatc tctgttcgcg gctttggtga agcggaatac tggttctcac tgatcaaagt
    16921 cacgacagtt attgtcttta tcatcgttgg cgtgctgatg attatcggta tcttcaaagg
    16981 cgcgcagcct gcgggctgga gcaactggac aatcggcgaa gcgccgtttg ctggtggttt
    17041 tgcggcgatg atcggcgtag ctatgattgt cggcttctct ttccagggaa ccgagctgat
    17101 cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac attccacgcg cggtacgtca
    17161 ggtattctgg cgaatcctgt tgttctatgt gttcgcgatc ctgattatca gcctgattat
    17221 tccgtatacc gatccgagcc tgctgcgtaa cgatgttaaa gacatcagcg tcagtccgtt
    17281 caccctggtg ttccagcacg cgggtctgct ctctgcggcg gcggtgatga acgcagttat
    17341 tctgacggcg gtgctgtcag caggtaactc cggtatgtat gcgtctactc gtatgctgta
    17401 caccctggcg tgtgacggta aagcgccgcg cattttcgct aaattgtcgc gtggtggcgt
    17461 gccgcgtaat gcgctgtatg cgacgacggt gattgccggt ttgtgcttcc tgacctccat
    17521 gtttggcaac cagacggtat acctgtggct gctgaacacc tccgggatga cgggttttat
    17581 cgcctggctg gggattgcca ttagccacta tcgcttccgt cgcggttacg tattgcaggg
    17641 acacgacatt aacgatctgc cgtaccgttc aggtttcttc ccactggggc cgatcttcgc
    17701 attcattctg tgtctgatta tcactttggg ccagaactac gaagcgttcc tgaaagatac
    17761 cattgactgg ggcggcgtag cggcaacgta tattggtatc ccgctgttcc tgattatttg
    17821 gttcggctac aagctgatta aaggaactca cttcgtacgc tacagcgaaa tgaagttccc
    17881 gcagaacgat aagaaataag tttcctccct tccttgctaa gccctctcaa ccgagagggc
    17941 tttttcaatt ccatttccct gacaaatcat gcggatataa aatttaatat ttggattgat
    18001 aattgttatc gtttgcatta tcgttacgac acaatcaaaa aaggctgaca aatcagaggc
    18061 tgttccggct ttctgggatg atcacctgca tacaaataag tccaccgcga tgctgccgta
    18121 cgcaagggga cgtaaagaag atgtgagcga tacccatttt attttcgtag ttacctcatg
    18181 gagatatgga atgtttaggt tgaacccttt cgtacgggtc gggctgtgtt tgtccgctat
    18241 ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc gaaacgatgg ttgtcactgc
    18301 atcttccgtg gaacaaaatc ttaaagatgc acccgccagt atcagcgtca ttacccagga
    18361 agacctgcag cgaaaaccgg tacagaatct gaaggatgtc ctcaaagaag tgcctggcgt
    18421 acaactgacg aacgaagggg ataaccgtaa gggcgttagt attcgtggtc tggacagcag
    18481 ctacaccctg attcttgtcg acggtaaacg cgttaactcc cgcaatgccg tcttccgcca
    18541 caatgatttc gatctgaact ggatcccggt cgattccatc gaacgtattg aagtggttcg
    18601 tggcccgatg tcgtcgctat acggctccga tgcgctcggc ggtgtagtga atatcatcac
    18661 caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc gataccacca ttcaggaaca
    18721 tcgcgatcgc ggtgatacct ataacggtca gttctttacc agtggaccat taattgatgg
    18781 tgtgctggga atgaaagctt acggcagcct ggcaaaacgt gaaaaggatg acccgcaaaa
    18841 ctcaacgacc accgataccg gagaaacgcc gcgtattgaa ggattctcca gccgcgacgg
    18901 caatgtcgaa tttgcctgga caccgaatca aaatcacgat tttactgccg gatacggttt
    18961 cgaccgtcag gatcgtgatt ccgattcgct ggacaaaaac cgcctggaac gccagaacta
    19021 ctgcgtcagc cataatgggc gttgggatta cggcaccagc gaactgaaat cctacggtga
    19081 gaaagtcgag aacaaaaacc ctggcaacag cagcccgata acttccgaaa gcaatacggt
    19141 cgacggcaaa tacacgttgc cgctgacggc gattaatcag tttctcacgg ttggcggtga
    19201 atggcgtcac gacaaactta gcgatgcggt gaacctgacc gggggaacca gctccaaaac
    19261 gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg cggatcttcg agccgctggc
    19321 gctgacgacc ggcgtgcgta tggacgatca cgaaacctac ggtgaacact ggagtccgcg
    19381 tgcctacatg gtttataacg ccaccgacac cgtaacggtg aaagggggct gggcgacggc
    19441 atttaaagca ccttctctgt tgcaacttag ccctgactgg acgagcaatt cctgccgtgg
    19501 cgcatgtaag attgtgggta gcccggatct gaaaccagaa accagcgaaa gttgggagct
    19561 ggggctttac tacatgggtg aagaaggctg gctggaaggg ggtgaatcca gcgttatcgt
    19621 tttccgtaac gatgtgaaag atcgtatcag catcagccgt acgtctgacg tcaacgctgc
    19681 accgggctac caaaactttg ttggttttga gacgggcgct aacggacggc gcataccggt
    19741 atttagctac tacagcgtta acaaagctcg tattcagggc gtggaaaccg aactgaaaat
    19801 tccgttcaac gatgaatgga aactgtcgat caactacacc tacaacgatg gtcgtgatgt
    19861 cagcaacggc gaaaacaaac cgctatccga tctgccgttc catactgcta acggtacgct
    19921 ggactggaaa ccgctggcgc tggaagactg gtcattctat gtttctggtc actataccgg
    19981 gcagaaacgc gccgacagcg cgacggctaa aacaccgggc ggttatacca tctggaatac
    20041 cggcgcggcc tggcaggtga ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg
    20101 cgacaaggat ctcagtcgtg acgactacag ctataacgaa gacggacgtc gttactttat
    20161 ggcagtggat tatcgcttct gatgagaaga tgcccggcga accgggcgga ctttcacttc
    20221 agtaaatact gcgcatggaa gcgcaggtga tcctctataa aagaggcgat gaagtagtaa
    20281 ctgtgatcat atcccggctg aatacgcagc gtcatcggcc acgctttctg gcgcgcggct
    20341 tctgccaata ccgcaggttg caactggtcg gcaagaaact gatcattatc gccctgatcg
    20401 ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca tcagcgcgca actatcccat
    20461 tccagcatgc atttttgtcc tcacctaaat aggtgctaaa cgctttgatt ccccacggga
    20521 cgctgcacgg attcacaatg ggcgcaaagg ccgaaacgct ggtgtattta cccggatttt
    20581 tcagcgccat aatcagcgca ccgtgaccac ccattgagtg accgctaatg gcgcagcggt
    20641 cgctgacatt aaattgcgac tgaaccagcg ccggtaattc atcgcgcaga taatcataca
    20701 tccggtaatg cgtcgcccac ggcggttgcg tggcattaag ataaaagcct gcgccctggc
    20761 ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg cgggctggtg tctggcatca
    20821 ccagtacaat ccccagttcc gccgctaccc gctgggcacc cgccttggtg gtgaagttct
    20881 cgtcattgca ggttaatccg gaaagccagt acagcactgg tggcggggtg tgatcacgag
    20941 ggggtgggag aaagatactg aacgtcatcg ggcagtttaa ggtactggag tcgtgtcgcc
    21001 agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg cgtggctcct
    21061 tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt gagtaacttt
    21121 cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag cggcaattac
    21181 aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat aatcaagctg
    21241 taaatcagct taataacttt gcccccacgc agggcggagg cgtcacacct gcaggagaaa
    21301 tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt agttgcgaga
    21361 ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac gcgcaaaagc
    21421 cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct ggctgatgac
    21481 agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat tttctccggt
    21541 ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa ggtcgatgaa
    21601 atggtcaccg tgcgcgatat cactctgacc agtacctgtg aacaccattt tgttaccatc
    21661 gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct gtcaaaaatt
    21721 aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc aggaacgtct gacgcagcaa
    21781 attcttattg cgctacaaac gctgctgggc accaataacg tggctgtctc gatcgacgcg
    21841 gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa ccagtgccac gacaacgacc
    21901 tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgagtttct gcgcgctgtg
    21961 cgccatcaca actgattaaa aggcaggaac catggagcgc aacgtcacgc tcgattttgt
    22021 tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg ggctaccaaa
    22081 ggcggcttat ctcaatcccg cctggtacga tgctattacg ccgcaggatg catggacctg
    22141 ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc tgctgtttgg
    22201 tgctggcctg caaatgttgc tgccccgtgg cagactctgg atccagtcgc ggttaacgct
    22261 gttagtcttg ttgggcttta ttcacggttt attgttctgg gacggcgata ttctgctggc
    22321 ttacgggctg gtggggcttg gcgttttgct gttattgggg ctgatttccg acagccagac
    22381 cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact ggaagcttca
    22441 cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact
    22501 gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg cgctgatgcg
    22561 cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg gttttgtgct
    22621 ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc agctggactg
    22681 ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg cgccgtttca
    22741 ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc gctttaagct
    22801 ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat tgcaaacgct
    22861 gatttgtacc acgctttttt accacctcgg tttgtttatg cattttgacc gcctggagct
    22921 gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta tctggctgcg
    22981 ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc gtgctgccgg
    23041 accggcaata tctaaaacat caagataacg atctggatta cattcgttaa caaaacggct
    23101 gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg ctgacaaaat
    23161 agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata tgatcaccat
    23221 tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa
    23281 cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga gtgagctgga
    23341 ttatcggcca aacgccaatg cccaggcgct ggcaactcag gttagcgaca ccattggcgt
    23401 ggtggtgatg gacgtttctg atgcgttttt cggcgcgctg gtaaaagcgg tggatctggt
    23461 cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag cggaaaaaga
    23521 gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg ttcactcaaa
    23581 agcattgagt gacgatgaac tggcgcaatt tatggataac attcccggta tggtgttaat
    23641 caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc
    23701 ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt atctttcttc
    23761 cagccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg cgttgaaaga
    23821 gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca tgccgggcgg
    23881 tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg tatttgctta
    23941 taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca ttgcgattcc
    24001 gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca ccgacccgca
    24061 attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg aactggcctt
    24121 gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc cgacgttagt
    24181 gcgtcgccat tctgtcgcaa cgcgccagaa tgcggcggcg atcactaact caacaaatca
    24241 ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcttaacaa tgtgatagct
    24301 atgattgcac cgttttaatg ttgtaacccg tatgtaacag tgaataatca ctttcaccga
    24361 ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg gcatggaaga
    24421 acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct actgccccgc
    24481 cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca tgaataagaa
    24541 ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg cacacgctgc
    24601 tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt ctgtagtgcg
    24661 caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga tgaatgattc
    24721 tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga aaggggtgaa
    24781 ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg agaaagcgcg
    24841 tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg cgctggatag
    24901 ctacgacaaa gcctactacg ttggcactga ctccaaagag tccggcatta ttcagggcga
    24961 tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag acggtcagat
    25021 tcagttcgta ctgctgaaag gtgaaccggg ccatccggat gcagaagcac gtaccactta
    25081 cgtgattaaa gaattgaacg ataaaggcat caaaactgaa cagttacagt tagataccgc
    25141 aatgtgggac accgctcagg cgaaagataa gatggacgcc tggctgtctg gcccgaacgc
    25201 caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc
    25261 actgaaagca cacaacaagt ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc
    25321 gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg ctaacaacca
    25381 ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg
    25441 caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg tagataaaga
    25501 caacttggct gaattcagca agaaataaga ctgattgggc gcagtcgttt actgcgccct
    25561 tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt cttccgggga
    25621 atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg cacttgataa
    25681 cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa acggtgcagg
    25741 aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg gcaccatttt
    25801 attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa atggtatttc
    25861 gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata acatgtggct
    25921 ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa
    25981 agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg gcacattatc
    26041 cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt
    26101 gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc tgttcactat
    26161 tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata aaatggaaga
    26221 aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga tcgccaccga
    26281 accgctggca ggactgacga tggacaagat catcgccatg atggttgggc gttctcttaa
    26341 tcagcgtttt cctgacaaag aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct
    26401 aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata aaggggagat
    26461 cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga cgttatttgg
    26521 tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca ataaccataa
    26581 tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc gctcaacggg
    26641 aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc gcaactacaa
    26701 aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt gggtgattga
    26761 ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa
    26821 tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat taatgctcga
    26881 tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt taattgccga
    26941 actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg agttgttagg
    27001 gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa
    27061 aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag attaggggct
    27121 tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg gcggtattta
    27181 cgtcgttctt ttagttttgc tggcgattat tattttccag gacccaacat ttttaagtct
    27241 gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg cgctcggtgt
    27301 ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg tagggctggc
    27361 ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag tgttcccgga
    27421 aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg gtgcggtgat
    27481 cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca ttaccacgct
    27541 cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg tcggggcgtc
    27601 gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg tcgcgctggg
    27661 gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt
    27721 gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta acccggaagc
    27781 ggcaaaaata tctggtgtca acgtcggcct gaacctgctg atgatctacg cgttgtctgg
    27841 cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg ccaccaacaa
    27901 cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt
    27961 cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt ttaccgtcat
    28021 caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca tcaaaggggc
    28081 gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga agtgatgtga
    28141 ttgcggatat tgccggatgc gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc
    28201 tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt gaccacaaag
    28261 cagacaaccc acacatttct cggtattaca atgcggtgtg cgggttttct cgctccattc
    28321 catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc caacacattt
    28381 gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg ccgggacaat
    28441 attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat cggcgagata
    28501 atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
    28561 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatctt cccaggtttc
    28621 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
    28681 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
    28741 accgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
    28801 ggtgttaatt gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
    28861 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
    28921 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
    28981 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag ctgctcccat
    29041 tgctgctcat tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttccttc
    29101 agccgacgca gggcggtcag attctcttcc aacggatgtt cagcaatctg ctccatattt
    29161 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    29221 ttggcgataa aaaagccgat cgttttaaac accacaccgc cccaacctgt gtcgtaggct
    29281 ttggcacaca tctcatagca gttgcctacc ggcgaagaag agaggcagaa cgggttggga
    29341 aagttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    29401 aatagtgatg aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt
    29461 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg atcgcgggtc tgataatttt
    29521 gtgttttgat ggtgttacgc tgctgctcta actccgcaaa ggcatccagt ctggcatgct
    29581 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcctgat aaccgtacat
    29641 gcttaaacgt gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa
    29701 cgcccagctc ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt
    29761 cacgtgctac gcaagttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    29821 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    29881 cctgtcgtac acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg
    29941 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30001 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30061 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30121 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30181 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30241 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30301 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30361 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30421 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30481 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30541 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30601 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30661 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaagctg tgaaaggatt
    30721 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30781 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30841 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    30901 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    30961 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31021 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31081 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31141 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31201 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31261 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31321 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31381 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31441 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31501 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31561 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31621 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31681 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31741 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31801 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31861 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    31921 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    31981 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32041 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32101 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32161 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32221 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32281 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32341 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32401 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32461 accactcaac caagcgtggc tgattgcgct ttgttcggca tgaacggttt gctgcattgt
    32521 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32581 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32641 tgcgaatgcc agcgcgtctt cgtccagtcc cgttgcgctc ttcagcgatg agacttgctc
    32701 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32761 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32821 tcatgtaatg ggataacagt ttacaaaccc gttatggctt tatatgtgat ctgaatctca
    32881 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    32941 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33001 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33061 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33121 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33181 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33241 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33301 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33361 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33421 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33481 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33541 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33601 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33661 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33721 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33781 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33841 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatctgcatc
    33901 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    33961 aggcaggcat aaatcaggat gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34021 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34081 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34141 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34201 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34261 gatatgcgtg tgttactggc accgatggag ggagtgcttg actctcaggt gcgtgaattg
    34321 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34381 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34441 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34501 aacgccgccc gtgcggtgga gttaggtttc tggggcgtgg atctcaattg cggctgcccg
    34561 tcgaaaacgg ttaacggtag cggcggcggg gcgacgttac tcaaagatcc tgaactcatc
    34621 taccaggggg caaaagcgat gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa
    34681 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    34741 gctggcgcta cggagctggt ggtgcatggg cggacgaaag agcagggtta ccgcgcggag
    34801 catattgact ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc
    34861 aacggtgaaa tctgggactg gcagagcgcg caacaatgca tggcgatcag cgactgcgac
    34921 gcagtgatga ttggtcgcgg ggcgttcaat attcccaacc tgagccgggt ggtaaaatat
    34981 aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg
    35041 gaaaagcagg gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat
    35101 ttgcgtaaag aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataac
    35161 tcccctgata ttgcaagggc tattcaggca attgatatcg agaaactcta aaaaccatag
    35221 tgatgttttt gacaatgtgt tagctcttta tcaataggcc gcacggttgt ttggtggcgt
    35281 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    35341 gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat aagccgaggg
    35401 caccgcgcag cggcgatttt gttcgccaga gcccgggggg gcagggggcg gcggcgattg
    35461 gtcgccccct gcgcgctcct tgcgccagtg attatatgaa gcaatgcaca tgacaggagc
    35521 gcaacaggat gagagactga aatattctcg ttattttcgg atctgatgac attaattaat
    35581 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
    35641 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgct gaataagtat
    35701 aggaaaaaac gaagtccttt gccgttatcc ataaacgatt aagataaatc ggaagatatc
    35761 atttacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
    35821 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc ttacctgatt
    35881 attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg ctgttactga
    35941 tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag caaacgcccg
    36001 ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca gacagccaat
    36061 ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca ctacagaacg
    36121 caccggatat gcaggtggct gaacagcgca ttcagctagc tgaagcccag gcgaaggcag
    36181 ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg caaaaaatgt
    36241 cggcagaagg gctaatgggg ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc
    36301 cgtggtacac caacggaact tttggcttaa cggcgggctg gcatctcgat atctggggaa
    36361 agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg
    36421 agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag tggcaaaccc
    36481 aggcggcgtt aaacacggtc ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga
    36541 ccgatcgcca gctatatcag aacgggatta cttcttcagt tgacggtgtg gaaaccgata
    36601 ttaatgccag caaaacccgg cagcagctca acgatgtcgc ggggaaaatg aaaattattg
    36661 aggcacggtt aaatgcactc acaaatcacc agactaagtc attgaagctt aaaccggtcg
    36721 cgttgccgaa agtggcaagc cagcttcctg atgaactggg gtactcctta ctggcccggc
    36781 gggcagattt gcaggcggcg cactggtacg ttgagtcatc gttaagcact attgatgcgg
    36841 caaaagcggc attttatcct gacatcaacc tgatggcctt cctgcaacag gatgcgttgc
    36901 acttaagcga tctgttccgt cattccgcgc agcaaatggg cgttacggca ggcctgacgc
    36961 tacctatttt cgatagtggt cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca
    37021 acttgtctat cgccagctac aacaaagcgg tggttgaagc agtgaatgac gtggcgcggg
    37081 cagccagtca ggttcagaca ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg
    37141 atgccttgcg tgtggtaggt cttgcgcagg cccgctttaa cgcgggcatc attgctggtt
    37201 cccgcgtcag cgaagccaga atcccggcgc tgcgtgagcg ggccaatggc ctgttattgc
    37261 aagggcagtg gctggatgcc tccattcgac tcaccggtgc gctgggcggg ggttacaaac
    37321 gctgatgata tgcgccttct atacttaacg tttattcagc gttaagtgga gaactcgatg
    37381 gcataggtcg cgataattac cgcttccgat tcggggatcg gcaaagagtg cgcgttatta
    37441 ctggcgcagc aggggtttga tattggcatt acctggcact cagatgaaga aggggcaaaa
    37501 gataccgcgc gtgaggtggt tacccacggc gtacgtgcgg agatcgtgca actggatctc
    37561 ggcaatctgc cagaaggtgc gcaggcgctg gagacactca tccagcgatt tgggcgtatt
    37621 gatgtgctgg tgaataatgc gggtgcaatg accaaagcgc cgtttcttga tatggctttt
    37681 gatgagtggc gcaagatttt taccgttgat gttgatggtg catttttatg ctcgcaaatt
    37741 gcggctcgtc agatggtgaa ataagggcag ggcggtcgca tcatcaacat tacgtcggta
    37801 catgaacata cgccgctgcc ggatgccagc gcctacacag ccgctaaaca tgcgctcggc
    37861 gggttaacca aaacgatggc gctggagttg gtcaggcata agattttggt gaacgcagtc
    37921 gcgcctgggg cgatagccac gccaatgaac agcatggatg atagcgacgt gaagcccgac
    37981 gcggagcctt cgattcctct acggcgtttt ggcgcaacgc atgagattgc cagcctggtg
    38041 gtgtggcttt gttcggaggg cgcaaattac acgacagggc agtcgttgat agtggatggc
    38101 ggctttatgt tggcgaatcc gcagttcaac ccagaatagc gcagtgaggt gcgtggatat
    38161 ttcaatgggt taggccagat aagacaccag cgtcgcatca ggcatggaac acccggtgcc
    38221 ggatgtggag tgaatgctta tctgtggcaa ctgctttacg cctgattatc cggctttttc
    38281 ttcccgcgcc gtttcagcca ccagcgcacg cccaccacca gaaccacgac cagaatcaac
    38341 cagacccagt gcttcaaatg ctggtcgaga ttgtgcaacc acggcgcaat cacctgacca
    38401 ccagcgtaac caatagtggt aaaaatcaac gcccaggcaa atgcgccgag aatattcagc
    38461 ggcagaaaga ttttcggcgg cagctggctg gcaccaatca gcgtcgggcc aatcacccga
    38521 aagccataca taaagcgcgt accaatgaca aacagatacg gatggcgttg gataagtttc
    38581 tgtgcccgct caattttatc ctgatgtttc gagaaacggc gtaacagctt gccgccaaac
    38641 cgccgcccgc acagatagag cacctggtcg ccaatcatgc caccaagcgc cacagaaagt
    38701 accaccagtg ggaactttaa tagcccctga tgtgccgcaa cgcctcccag caaagtcacg
    38761 gtttcacctt ccgccaggct accgataacc agcgcggcat aaccatattg tgagatgagt
    38821 gtattgagag ccataggcca gtttgtctcc ctttggttgc ctgtaagcat acaccctgtg
    38881 aatcattcac gctgaaaaag gacatacatc agacggtgca aattacatgg atgaacaaaa
    38941 aataatcaca agctggctta tacttgaact gtcaggttca tgaactcaca acaagggggc
    39001 ggtatgagcc atgtctgggg actgttttcc catcccgatc gtgaaatgca ggtgattaat
    39061 cgcgaaaacg aaacgatttc tcaccattat acccaccacg ttttgctgat ggcggcgatt
    39121 ccggtgattt gcgccttcat tggcactaca cagattggct ggaattttgg cgatggcact
    39181 atcctgaagt tatcctggtt taccggactg gcgctggctg tcttgtttta cggcgttatg
    39241 ctggctgggg ttgcggtgat ggggcgggtc atctggtgga tggcgcgcaa ttacccgcag
    39301 cgtccgtctc ttgcgcactg catggtcttc gcgggctacg ttgcaactcc gctgttttta
    39361 agcggtctgg tggcgcttta tccactggtc tggttgtgtg cgctggtcgg cacggtggcg
    39421 ctgttttaca ccggctacct gctttacctt ggtattccga gcttcctgaa tatcaacaaa
    39481 gaggaaggcc ttagcttctc cagttcgacg ctggcgattg gcgtgctggt actggaagtg
    39541 ctgctcaccc taaccgttat actatggggc tacggttatc gtttgttcta gaaagttctt
    39601 tgacgttgca ttgctggcgt aaatgtcagc aatgcagcat ttactcacga ttctcctttg
    39661 acgatcgcac tttttgctcg ttatgatggc gtcgctagcc tcagtaaatc cttactccgg
    39721 cggtgcgcaa cccgtgcgcg tgaaccacta tctgaatgct catcatgccg aaatttcgag
    39781 tttctttatt tagcttggcc ctgatgctgg ctgtgccttt tgcaccgcag gccgttgcga
    39841 aaacggcagc cgctaccacc gcttcacaac cggaaattgc ctccggtagc gcgatgattg
    39901 ttgatctgaa taccaacaaa gtgatctatt cgaaccaccc ggatctggtg cgtccgattg
    39961 cgtctatcag caaattaatg accgcgatgg ttgtgctgga tgcacgactg ccgctggatg
    40021 aaaaactaaa agtggatatc agccagacgc cggagatgaa aggggtctat tcgcgcgtac
    40081 gactgaatag cgaaatcagc cgtaaagata tgctgttgct ggcgctgatg tcttcagaaa
    40141 accgcgcggc ggcaagcctt gcgcaccatt atcccggtgg ttacaaagcc tttattaagg
    40201 caatgaatgc gaaagcgaaa tcgctcggaa tgaacaacac gcgctttgtt gaacctaccg
    40261 gattgtcggt gcataacgtt tcaactgccc gtgacttaac caaactgctc attgccagca
    40321 aacaatatcc gttgatcggg cagttaagta ctacccggga agatatggca accttctcta
    40381 acccgacgta tacgctgccg ttccgcaata ctaatcatct ggtgtatcgc gataactgga
    40441 atattcagtt aaccaaaacc ggctttacca atgcggcggg ccattgtctg gtgatgcgta
    40501 cggttatcaa taataaaccg gtggcgctgg tagtgatgga cgcgtttggc aaatataccc
    40561 attttgccga tgccagccgc ctgcgtacct ggattgaaac cggtaaagtg atgcctgtgc
    40621 cggcagcagc gttgagctat aaaaaacaaa aagccgccca aatggcggcg gcggggcaga
    40681 cggcacagaa cgattaagtg aattcggatg gcgatactct gccatccgta atttttactc
    40741 cacttcctgc cagtttttcc gtttactggt tttaccgatc cccggattca tgctattggt
    40801 cggatcattc tcgcgataga acttctgcaa cgtctccggt gctttataca aatgaccgac
    40861 gttatgctcg gcagggtact gcgcgccgcg ctgctgtagc agttccagca tctgttcttt
    40921 taacgcatgc acatccacgc cttttttcac tatgtaatcc tgatggaaga catagcacat
    40981 aaaatggccg taatagagct tgtgcaccag ctggctgtcg atctccggcg gtaaatgctc
    41041 ataccactcg gtgtcgttac gccgcagagc gatatccagt gccagaatgt cttcgacttc
    41101 atcggaatgc accgcctgat aacgaattgc tgcgcccgca gcggcgaaac ggtgtaaaaa
    41161 cgctttgcta ccttcctccg gcgtacagac aaagaaatcg ccctcggcct gtttgaaata
    41221 atccaccagc cacgatttgg cttcaccgac gccatcgcct gccattttta acagcagatg
    41281 atgctcgtat ttatcgcgcc agtttttcat gcgcggcggt aaatggctgg ggaacaggtg
    41341 accgaatttt tgcattgcac ggtcagtaaa atgcggacgg aagaatttca ctttctccag
    41401 catcgcatcg gtgcgtccct tgagattaaa gaagaacggc atcttgtcgg tgcctaactt
    41461 atcaatcatc aggaaggtgt ctttgccgta tttttccgca atatcgtaga tatcccggtg
    41521 catatattcc ccggcaaccg gcagattttc gaagttagcc agaatatgac ggcggatttc
    41581 ggtcagcact tccggctggt tggtgccgat ataaaacacc tgctgatttt tttccgcttc
    41641 gaaggtatca agacgtaccg caaagacggc cagcttcccg gcgcaaccag aagattcaaa
    41701 taaccgatcc ggatcggcgt tataacgtgc gggcgtgtcg gcttcaatat cacgaacgcg
    41761 gtggacatag tcataatcgt gggcgtgacg accatcgtga cgcacatcgt catctttgat
    41821 gcgatcgtca tccagcttgc taaggatctg ctccggcgtt tcgcccagat caatccccag
    41881 atggttcacc agcgtcagtt tgccatcttc atctatacgc gcgaataacg acatttcggt
    41941 atacgccggg ccacgttgca ccagcgagcc gccggagttg ttacaaatac cgccgatgac
    42001 cgatgcgcct atacacgatg atccaatcac cgaatgcggt tcgcgtccca gcggtttgag
    42061 ggctttttcc agcgaataga gcgtggtgcc cggataggcc agcacctgtt cgcccttgcc
    42121 gagaacgtgc agcttgtcga gacgcagggt gctgatgatc acgatatcgc gatcatagtc
    42181 gttaccgttt ggcgtcgatc cttcggtcag gcctgtattg gcagcctgca tcagaataat
    42241 tttgtcggcg gtgacgcagg ctttcagcac ccgccacaat tccagtagtg agccagggaa
    42301 aacgacagcc agcgcgtcgc cctgaccaga acggaagccc ttgcgatagc gggccgtttt
    42361 tgcgggatcg gtgagcaggt gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc
    42421 tttattatca gttgttgtca tggaagacat tttccactcc ttgtggtggc gaaaaaatat
    42481 cacaaactag aatagcgcct tgtatgacaa ttcagcgagt gaatattttg aaaattcaga
    42541 caataaactg agcattcttt ttctctctcc atcatgctta tggcaaacta cggtttttgc
    42601 accattttcg ctgccacgtc gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt
    42661 tcagtaggaa tcgcggtgag tctggtcctg cagccagcac tggcggatga tttattcggt
    42721 aaccatccat taacgcctga agcgcgggat gcgttcgtca ccgatttgct caagaaaatg
    42781 acggttgatg agaaaattgg tcagctgcgc ttaatcagcg taggcccgga taatccgaaa
    42841 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc
    42901 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct
    42961 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt
    43021 ctggcctcgt cttttaacct cgatgcggtg aaaacggtcg ggcgtgtctc tgcttatgaa
    43081 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg
    43141 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aataatgggt
    43201 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgttactc ggtgatgacc
    43261 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc
    43321 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac
    43381 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc
    43441 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt
    43501 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat
    43561 gcggtgcgcg tggcgctgaa atccggaatc aacatgagta tgagcgacga gtactactcg
    43621 aagtatctgc ctgggttgat taaatccggc aaagtgacca tggcagagct ggacgatgct
    43681 gcccgccatg tactgaacgt taaatatgat atggggttgt ttaacgaccc atacagccat
    43741 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa
    43801 gaagcgcgtg aagtggcgcg cgaaagcctg gtgttgctga aaaaccgtct cgaaacgtta
    43861 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac
    43921 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg
    43981 attaaaaatt ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc
    44041 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg
    44101 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg
    44161 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc
    44221 actattccgc aaggccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg
    44281 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat
    44341 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg
    44401 tttggcgatt acaacccgtc cggcaagctg ccaatttcct tcccgcgttc tgtcgggcag
    44461 atcccggtgt actacagcca tctgaacacc ggtcgtccgt ataatgccga caagccgaac
    44521 aaatacactt cgcgttattt tgatgaagct aacggagcgc tttatccgtt cggctatggt
    44581 ctgagctata ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt
    44641 gacggcaaag tgacggccag cgtgcaggtg acgaacaccg gtaagcgcga gggggccacg
    44701 gtagtgcaga tgtacttgca ggatgtgacg gcttccatga gtcgccctgt gaaacagctg
    44761 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc
    44821 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag
    44881 ttcaatgtat ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg
    44941 taatgcttta gtaagggcgc gacgtttgcg ccctttgcct gatgttcata tcttaacttg
    45001 ctgaattttc gccctttgta ggccggataa ggcgctcgcg ccgcatccgg caggaaacgc
    45061 cggaccgaat atgcctctta agtatttatc tgattctgca actgaatcct tccgctcagg
    45121 ctaactctgt cattatcaac tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga
    45181 atgaggaaag catcatgcca ctcttaaagc tctgggcagg ttcactggtt atgttggcag
    45241 ccgtgagcct gccgctacag gcggcttccc ccgttaaagt cggttcaaaa atcgataccg
    45301 aaggcgcgct gctcggcaac atcatctttc aggtactcga aagccacggc gtaccgacgg
    45361 tgaataaagt gcaactcgga acgactcctg tggggcgcgg ggcgattact tccggtgaac
    45421 tggatatcta tccggaatat accggcaatg gcgctttctt ctttaaagat gaaaacgatg
    45481 cagcgtggaa aaatgccggg cagggctacg agaaagtcaa aaagctcgat gcggagcaca
    45541 acaagttaat ctggctgacg cccgcgcctg caaataacac ctggactatc gccgtgcgtc
    45601 aggatgtggc agagaaaaac aaactcactt cgcttgccga cctgagtcgt tatctgcaag
    45661 agggcggcac cttcaaactg gcagcctcgg cagagtttat cgaacgcgcc gatgcgttac
    45721 ccacgtttga aaaagcctat ggctttaagc tcggtcagga tcagttgctg tcactggctg
    45781 gtggcgacac ggcggtgacg attaaagccg ctgcccagca aacctctggc gttaatgccg
    45841 caatggctta cggcactgac ggtccggtcg cggcgctggg gctgcaaacc ttaagcgatc
    45901 cgcaaggcgt gcaacctatc tacgcgcctg caccagtggt gcgtgaatcg gtgttgaagg
    45961 agtatccgca aatggcacag tggctacagc cagtcttcgc cagcctcgat gcaaaaacat
    46021 tgcagcaact gaatgccagc attgcagtgg aaggactgga tgccaaaaaa gtggctgccg
    46081 actacctgaa acaaaaaggg tgggcgaagt aatttcccgt gacttatcta cgtattaatc
    46141 ctgttctggc gctgctgttg ttgctgacgg caatcgcagc ggcgctgccg tttatcagtt
    46201 acgcgcctaa tcgtttagtc tcgggtgaag ggcttcatct ctggcaactg tggccgcaaa
    46261 cgatctggat gctggtgggc gttggttgcg cctggctgac agcctgtttt gtccccgcta
    46321 aaaaaggcag catttttgca ctcattctgg cgcaattcgt cttcgtattg ttggtgtggg
    46381 gagctggaaa ggcggcgacc caactggcgc aaaatggcag tgcgctggcg cgtaccagcc
    46441 tcggcagagg tttctggctg gctgcggcgc tggcattgct ggcctgtagc gatgccatcc
    46501 gccgaatctc cacgcatccg ctgtggcgct ggttgttgca tatgcagatt gccattattc
    46561 cgctgtggtt gctgtactcc ggcacgctta acgatctctc actaatgaaa gaatacgcca
    46621 accgtcagga tgtgtttgac gacgcgctgg cacaacatct gacgttgctg tttggtgcgg
    46681 tgctgcctgc gttagtgatt ggtgtgccgt tgggcatctg gtgctacttt tccactgcgc
    46741 ggcagggggc aattttttct ctgctcaatg tcattcagac cgtgccttcg gtggcgctct
    46801 ttggcctgtt gattgcgccg cttgccgcgc tggttacggc ctttccgtgg ctggggaagc
    46861 tcggcatagc aggaaccgga atgacacccg cactgattgc gctggtgctc tatgccttgc
    46921 tgccgctggt gcgaggcgtg gtagtcggtt tgaaccagat cccgcgcgat gtgctggaga
    46981 gcgccagagc gatggggatg agcggggcgc agcgtttcct gcatgttcag ttaccgctgg
    47041 cgttactggt atttctgcgc agcctgcggg tggtgatggt gcaaactgta ggtatggcgg
    47101 tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct ggttttccag gggctgctaa
    47161 gcagcgccat tgatttagtg ttgctggggg tgatcccggt aattgttctg gcggtgctta
    47221 ccgacgcgct gttcgatttg cttatcgcac tgctgaaggt gaaacgtaat gattgaattt
    47281 agccatgtca gcaaactgtt cggcgcacaa aaagccgtta acgatctcaa tctcaatttt
    47341 caggaaggga gtttttcggt gctgattggc acatctggct ccggcaaatc caccaccctg
    47401 aaaatgatta accgcctggt ggagcatgac agcggagtga tccgctttgc cggagaagaa
    47461 attcgctcgc tgccagtact ggagttgcgc cgccggatgg gctatgccat tcaatctatt
    47521 ggcctgttcc cccactggag cgtggcgcaa aacatcgcta ccgtgccgca attacaaaaa
    47581 tggtcgcggg cgcggattga cgatcgtatc gacgaattaa tggcgctact ggggctggag
    47641 tcaaatttgc gtgagcgtta tccgcatcag ctttccggtg gtcagcagca acgtgtggga
    47701 gtggcgcgtg cactggctgc cgatccgcaa gtcttactga tggatgaacc ttttggcgca
    47761 ctggacccgg taacgcgcgg cgcgttgcaa caagagatga cgcgcattca ccgtttgctg
    47821 gggcgcacta ttgtgctggt cactcatgat attgatgagg cgctacggct ggcagaacat
    47881 ctggtattga tggatcacgg tgaagtggtg cagcagggga atccgctgac gatgctgact
    47941 cgtccggcga atgattttgt ccgccagttt tttggacgta gtgaactggg ggtgcacctg
    48001 ctttcgttac gtagcgtggc ggattacgtg cgtcgcgaag aacgggcaga aggtgaggca
    48061 ctggcagaag agatgacgct acgcgatgcg ctctccctgt ttgtcgcgcg gggatgcgag
    48121 gtgctgccgg tggtgaacac gcagggccag ccttgcggca cgctgcattt tcaggatctg
    48181 ctggtggagg cgtaagcgta tgaagatgtt gcgcgatccg ctgttctggc tcattgctct
    48241 ttttgtggcg ctgattttct ggctgcctta cagccagccg ctgtttgctg ccttgttccc
    48301 acaactgcca cgacccgttt atcagcaaga aagttttgca gctctggcac tggctcattt
    48361 ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt ggcacaggtg ccggaattgc
    48421 tgttactcgc ccgtggggcg cggaatttcg cccactggtg gaaactattg ccgccgttgg
    48481 acagactttt ccgcctgtcg cagtgctggc gattgccgtt ccggtgatcg gctttggtct
    48541 gcaaccagcg attatcgcct tgatccttta cggtgtgctg cccatcctgc aggtgacact
    48601 tgccgggctg ggagcgattg atcccagcgt gacagaagtt gcgaaaggta tgggaatgag
    48661 tcgaggtcag cgactgcgta aggtcgagct accgctggcg gctccggtga ttctggcggg
    48721 cgtgcgaact tcggtgatta tcaacattgg tacggcgacg atcgcctcaa cggtaggggc
    48781 cagtacgctg ggtacgccca tcatcatcgg gcttagcgga tttaataccg cgtatgtgat
    48841 ccagggggcg ttactggtgg cactggcggc gatcatcgca gaccgcctgt ttgaaaggct
    48901 ggtgcaggcg cttagccagc acgcaaaata aaggtataac ctgcgagcat gacgccacca
    48961 attccgccta acgccataaa caggaacagg gcgatgaccc caattttagc tatgcgcata
    49021 atgcactcct tatgttaacg aaaggattgt acagtaaagc gcatttgtta acgaatcatt
    49081 aaataccgag tgggaaaata tcatggcctt gttcttgcca actggtgagt tgctgctgtt
    49141 gggcggaggt tcgattttca cc
//
LOCUS       DBKELA010000017        47451 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.017, whole genome shotgun sequence.
ACCESSION   DBKELA010000017 DBKELA010000000
VERSION     DBKELA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.017"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..79
                     /locus_tag="ACUQR0_000969"
     CDS             <1..79
                     /locus_tag="ACUQR0_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108116.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACUQR0_000970"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACUQR0_000970"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HHV9108117.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACUQR0_000971"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACUQR0_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HHV9108118.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACUQR0_000972"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACUQR0_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HHV9108119.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACUQR0_000973"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACUQR0_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HHV9108120.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACUQR0_000974"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACUQR0_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HHV9108121.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACUQR0_000975"
     CDS             5377..5835
                     /locus_tag="ACUQR0_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HHV9108122.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACUQR0_000976"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACUQR0_000976"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HHV9108123.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACUQR0_000977"
     tRNA            6887..6971
                     /locus_tag="ACUQR0_000977"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACUQR0_000978"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACUQR0_000978"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACUQR0_000979"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACUQR0_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HHV9108124.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACUQR0_000980"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACUQR0_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HHV9108125.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACUQR0_000981"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACUQR0_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HHV9108126.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACUQR0_000982"
     CDS             complement(10252..13995)
                     /locus_tag="ACUQR0_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HHV9108127.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACUQR0_000983"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACUQR0_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HHV9108128.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="ACUQR0_000984"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="ACUQR0_000984"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HHV9108129.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="ACUQR0_000985"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="ACUQR0_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HHV9108130.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACUQR0_000986"
                     /gene_synonym="iatA"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACUQR0_000986"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HHV9108131.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="ACUQR0_000987"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="ACUQR0_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HHV9108132.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACUQR0_000988"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACUQR0_000988"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HHV9108133.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACUQR0_000989"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACUQR0_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HHV9108134.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="ACUQR0_000990"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="ACUQR0_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HHV9108135.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="ACUQR0_000991"
     CDS             23392..23499
                     /locus_tag="ACUQR0_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HHV9108136.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACUQR0_000992"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACUQR0_000992"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HHV9108137.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACUQR0_000993"
     CDS             complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACUQR0_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HHV9108138.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACUQR0_000994"
     CDS             complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACUQR0_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HHV9108139.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACUQR0_000995"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACUQR0_000995"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHV9108140.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACUQR0_000996"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACUQR0_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HHV9108141.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACUQR0_000997"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACUQR0_000997"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HHV9108142.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="ACUQR0_000998"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="ACUQR0_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HHV9108143.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="ACUQR0_000999"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="ACUQR0_000999"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HHV9108144.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="ACUQR0_001000"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="ACUQR0_001000"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HHV9108145.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="ACUQR0_001001"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="ACUQR0_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HHV9108146.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACUQR0_001002"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACUQR0_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HHV9108147.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="ACUQR0_001003"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="ACUQR0_001003"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HHV9108148.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="ACUQR0_001004"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="ACUQR0_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HHV9108149.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36789..39656
                     /locus_tag="ACUQR0_001005"
     CDS             36789..39656
                     /locus_tag="ACUQR0_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHV9108150.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSPDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="ACUQR0_001006"
     CDS             complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="ACUQR0_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HHV9108151.1"
                     /translation="MSGAFNNDGRGISPLIATSRERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41903..42973
                     /gene="dhaK"
                     /locus_tag="ACUQR0_001007"
     CDS             41903..42973
                     /gene="dhaK"
                     /locus_tag="ACUQR0_001007"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HHV9108152.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42984..43616
                     /gene="dhaL"
                     /locus_tag="ACUQR0_001008"
     CDS             42984..43616
                     /gene="dhaL"
                     /locus_tag="ACUQR0_001008"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HHV9108153.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43627..45045
                     /gene="dhaM"
                     /locus_tag="ACUQR0_001009"
     CDS             43627..45045
                     /gene="dhaM"
                     /locus_tag="ACUQR0_001009"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HHV9108154.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45365..47062
                     /gene="treA"
                     /locus_tag="ACUQR0_001010"
     CDS             45365..47062
                     /gene="treA"
                     /locus_tag="ACUQR0_001010"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HHV9108155.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47141..47451)
                     /gene="ycgY"
                     /locus_tag="ACUQR0_001011"
                     /pseudo
     CDS             complement(47141..47451)
                     /gene="ycgY"
                     /locus_tag="ACUQR0_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
BASE COUNT        11897 a        12234 c        11637 g        11683 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcta
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccac ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aagcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaagc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ttggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta cgacaactta gggaataact cgatataaac tcacctttgt
    36601 aagggggggg ttaaatatat attaattcct gtgttcctga ttttgcccgg taatagagaa
    36661 ttattctcag agaagctcat catgattgca ccatacagat atcatgatga taagaggata
    36721 ttattcattg acttaaacaa aatgacaaaa tccatagacc catagcctgg gtactatata
    36781 ggagtggtat gggcatcaaa caacacaatg ggaataccaa agccgatcgt ctcgctgaat
    36841 taaaaatccg ttcgccctca attcaactga taaaatttgg cgctattggt ttgaatgcaa
    36901 ttatcttttc ccccctgctg atagctgctg atacaggaag tcaatatggc accaatatta
    36961 ctattaatga tggtgacaga attacaggag ataccgccga tccatcagga aacctctatg
    37021 gtgtaatgac cccagcagga aacacgcctg gcaatatcaa cctgggtaat gatgtcaccg
    37081 tcaatgtcaa cgacgcctct ggatatgcaa aaggaatcat tattcagggc aaaaacagct
    37141 ccctgacagc taaccgactc acagtagatg ttgttggtca aacctctgcc atcggcatta
    37201 acttaattgg tgactatacc catgctgact taggcacagg cagcaccatt aagagtaacg
    37261 atgacggcat cattattggg catagctcaa cactaacagc cactcaattc accattgaaa
    37321 actcgaacgg tataggccta accatcaatg actatggcac cagtgtcgat cttggaagcg
    37381 gaagtaaaat caagaccgat ggaagtacag gtgtttatat cggtggtctc aacggcaata
    37441 acgccaatgg tgctgcgcgt tttacggcga cagacctgac aatcgatgtt cagggctaca
    37501 gcgccatggg gataaacgta cagaaaaact ctgttgtcga tctcggaaca aacagtacca
    37561 ttaaaaccaa tggcgataat gctcacggcc tctggagctt tggccaggtt agcgcgaatg
    37621 cactcactgt tgatgtaact ggagccgcgg ccaatggcgt cgaagttcgt ggtggtacaa
    37681 ccactatcgg tgcagatagc catatttctt ccgcgcaggg cggtggcctc gtcaccagta
    37741 gtccagacgc gacaatcaat ttttctggca cggcagcgca acgaaacagc atcttttccg
    37801 gcggttctta tggtgcctcg gcccagacgg caacggctgt tatcaacatg caaaataccg
    37861 atattacggt tgatcgtaat ggcagtctgg cgctgggttt gtgggcgctc agcggcggta
    37921 gaataaccgg agacagtttg gctatcaccg gcgcggcagg agccagaggg atttatgcca
    37981 tgaccaacag ccagatcgac ctcacgagcg atctggtcat tgatatgagt acacccgacc
    38041 agatggccat cgcaacgcaa catgacgatg gttatgccgc cagccgcatc aacgcctcgg
    38101 gtcgtatgct tatcaacggt agcgttcttt ccaaaggtgg gctaatcaat ctggatatgc
    38161 accctgggtc ggtttggaca ggttcctccc tcagcgataa tgtcaatggc gggaaactgg
    38221 acgttgcaat gaataacagc gtctggaacg taacaagtaa ttctaatctc gacacgctgg
    38281 cgctgagcca ttcaactgtc gattttgcca gccacgggtc aactgccggc acatttacca
    38341 cattaaacgt agagaacctg agcggtaaca gtacctttat tatgcgtgct gatgttgttg
    38401 gcgagggtaa tggcgttaat aatagagggg atttattgaa tatcagcggg agtagtgctg
    38461 gtaatcacgt attggctatc cgcaaccagg gcagcgaggc cacaacggga aatgaagttc
    38521 tgacagtggt aaaaaccact gacggcgcgg cctcgttcag cgcgtcttct caggttgagt
    38581 tggggggata tctgtacgat gtgcgtaaaa atggcactaa ctgggagctt tacgcttccg
    38641 ggacagttcc ggaaccgact cctaatcctg aacccacacc agctcccgct cagcctccca
    38701 tagtcaaccc cgatcctacg cctgaacccg ctcccacgcc taaacccacc acgaccgcag
    38761 atgctggcgg caattatctc aatgtcggtt acttattgaa ctatgttgaa aaccgtacgc
    38821 tgatgcaacg gatgggtgac ttgcgaaatc agagtaaaga cggtaatatc tggttgcgca
    38881 gttatggggg aagcctggac tcctttgcca gtggcaaact gagcggcttt gacatgggtt
    38941 acagcggtat ccagtttggt ggggataaac gtctctctga tgtaatgccg ttgtatgtcg
    39001 gtctgtatat tgactcaaca catgcatcgc cggactatag cggaggcgac ggtaccgcac
    39061 gttcagacta catgggaatg tacgccagtt acatggcaca aaacggtttt tacagcgatc
    39121 tcgttataaa agcatcgcgc cagaaaaata gtttccacgt actggacagt cagaacaacg
    39181 gcgttaacgc caacggcact gcgaatggaa tgagcatctc cctggaagcc gggcagaggt
    39241 tcaacctgtc ccctactggt tatgggttct atatagagcc gcaaacccag cttacataca
    39301 gccaccagaa tgagatggct atgaaggcga gtaatggcct caatatacat ctgaatcact
    39361 acgaatcgct gctggggcgt gccagcatga tactggggta tgacatcacc gcaggcaaca
    39421 gccagctgaa tgtctatgtg aagactggcg ctatccgcga gttttcaggg gataccgaat
    39481 atctgttgaa cgactcccgg gagaagtaca gtttcaaagg taatggctgg aataacggcg
    39541 tgggagtcag tgcacagtat aacaaacagc acacattcta tctcgaagcg gattacacgc
    39601 agggtaacct ctttgatcag aagcaagtca acggaggata tcgcttcagc ttttaagtgc
    39661 gggatagaca agcccttaaa cgatgctgaa aattgttagc gtgcgccgga atgaaataca
    39721 gatcactctg atgtatcaga aatcgtttct gtctttcata cccggcgctt aaactgccct
    39781 gcatcaatgc catgttgctt cattttccgc cacagcgtag tgcggccgat cccaagtaaa
    39841 gccgacattt cctgaatgcg accgcctgtg acctgggctg cgttaataat tgcctctttt
    39901 tcaacttccg caaatgacag actggtggaa aggcgggtgg cgctgacatc atctgtcgcc
    39961 tgctcggtaa acagatgttc aggtaaatca ctgacgcgaa tgcgcccgtt atcactactc
    40021 agagcaagat tctcgatgac gctgtaaagt tcaaaatcgt tgcctggcca tgcacaagaa
    40081 actaggcgag cgagggcatc gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga
    40141 ctgcgtaatt tgttattcac cagcgccgga atgctgccac gccgcatacg cagaggcggg
    40201 atggtaattt caaatgcatg cagcgcgtaa tacagctggc gactaaaacg attttgttcc
    40261 accagcattg cgaggtccac ggtcgttgtt gcaatcactt tgacatcaat tggtattaaa
    40321 cgccgcgcat ccagtcgcgt gataaccccc tgcttgataa cctgcagcaa agcagactgt
    40381 aactccaccg ccagatattc aatcttttca agaaacagcg tgccgccgtg tgccagttcc
    40441 agccgactca gacggccatt ttcattgtcc gtgcgatcgc caccaataaa ttcttccgcc
    40501 agcgcagcat caccatataa ctcacaattg acggcgatat aaggacctgc agcacgctcg
    40561 ctttcattat gaattgcctg acttagcagt gccttgccca cgccctcttc tccacaaagc
    40621 aggacaggaa agctactgcg cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc
    40681 ggatcgtcct gtggcatatg agcgaaggta tggctgactt ttcctaattg actggtcatt
    40741 aactgccgca tctgttccac aggatggagc aacaaaataa agctggttcc ctgcgtttct
    40801 attatcggtt taagggttat caccgcatca ataaactggt gctggctttc aaaggttgct
    40861 tctacgtgtt tgagcggatg tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt
    40921 aagagttcag tgattgcccg tccctgactt gccgtcgcgt caaggcgcaa gacccgcgcc
    40981 gcctgggcat taataaattg caaattaccc tgctcgtccc agctaatcac gccatcatcc
    41041 atactttcta acagggcatt aagttgattt aaatgacggt tagtttcagc gagcaaactg
    41101 tccgtcagca gtaaattacc gacctcgcgg gcgattgcca acgtcaacgg caaatcagct
    41161 gcggtagttt gctcaaccgg acacgccagc gctattgttc ccgtcaatcg gcccttgctg
    41221 tcaaacaacg gcgttgcaca aaaggcccag ttccagagtg cctgtttgaa atgttgatcg
    41281 gccatcgttt tcacggcctg accagagata gccgctaacg atagcgcaca agtaccaata
    41341 attccctcgg cgcaatacgt gccgtcattg aatcccagtg cacttagctg ctgcaaggtt
    41401 tgcggatcgc cattacggct gagaatgcag gcggtttcat cgaggataaa cagcgcacac
    41461 tctcgcggtg ccatatattc ccaggcatct tccagcgcag cctgcccgag cgtcagcatc
    41521 gctttcttac gccgataaat agaagcaaat gtcacgccct gggcctgatg tggtacgttc
    41581 catgtctccc gtttcatcag cttattgcat cgctccctgg aggttgcaat taagggagat
    41641 atgccccgac catcgttgtt aaaagcgcca ctcatatccc gcatccctta tgacaaaagg
    41701 tgttccggct cgcaatttta agtattaata cacgtaaaag tttcaatatg gaacattcat
    41761 taaagcatat gtttcactat gaaacataat ttgggctaat tttcttttcc cttgcctgat
    41821 gcacaatgga ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta
    41881 ccgtaattgc tggagcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga
    41941 cgaacaactg gcaggactgg cgaaagcgca tccatcgctg acactgcatc aggatccggt
    42001 gtatgtcacc cgagctgatg cccctgttgc aggaaaagtc gccctgctgt cgggtggcgg
    42061 cagcggacac gagccgatgc actgtggtta tatcggtcag gggatgcttt cgggggcctg
    42121 tccgggcgaa attttcacct caccgacgcc cgataaaatc tttgaatgct ccatgcaaat
    42181 tgatggcggc gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt
    42241 tgaaacagcg accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga
    42301 tgacgacgtt gcggtaaaag acagtcttta taccgccggg cgacgcggcg ttgccaacac
    42361 agtattaatt gaaaaactcg taggcgcagc ggcggagcgt ggcgactcac tggaggcctg
    42421 tgcggaactg gggcgtaagt tgaataatca aggccactca ataggtatcg ctctcggtgc
    42481 ctgtaccgtt cctgccgcgg gcaaaccttc ttttaccctt gcggataatg agatggagtt
    42541 tggcgtcggc attcatggtg agccaggtat tgaccgccgc cctttctctt cccttgatca
    42601 aaccgtcgat gaaatgttcg acaccctgct ggaacatggc tcataccatc gcactttgcg
    42661 tttctgggat tatcaacaag gcagctggca ggaagaacca caaaccaaac aaccgctcca
    42721 gtctggcgat cgggtgattg cactggttaa caatcttggc gcaactccgc tttctgagct
    42781 gtacggcgtc tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgaacg
    42841 taatttaatt ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact
    42901 gaaagttgat gacgaaacgc tggctctctg ggacgccccg gtccacaccc cggcccttaa
    42961 ctggggtaaa taaggagaaa gcaatgtcac tgagcagaac tcaaattgtt aactggctca
    43021 ctcgttgtgg cgatattttc aacaccgaga gcgagtatct taccgggctg gatcgcgaga
    43081 ttggcgacgc tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac
    43141 tccctgctat cgcagataaa gatatcggtt tcattctcaa aaataccggt atgacgctgc
    43201 tttccagcgt cggtggtgcc agtggtccgc tgttcggtac cttctttatc cgcgccgcac
    43261 aggcgaccca ggcacggcaa agcctgacac tggaagagct ttatcagatg ttccgcgatg
    43321 gcgcggacgg cgtaatcagt cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg
    43381 tgtgggtgcc ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctgttccgg
    43441 tggcgctcga agctgccagt agcatcgccg aatccgctgc acaaagtacg attacgatgc
    43501 aagcccgcaa aggccgcgcc agttatctcg gtgaacgcag tattggtcac caggatcccg
    43561 gcgcgacctc ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa
    43621 ttggtgatgg taaacctggt catagtttca catagcagcc gactgggaga aggtgtcggt
    43681 gaattagccc gtcagatgtt aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt
    43741 gacgatccac aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct
    43801 gttgctgatg ccgaccatgt gctggtcatg atggatatgg gtagcgcatt attgagtgct
    43861 gaaactgcgc tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgcg
    43921 ccgttggtcg aaggtacact ggcagcaacg gtcagcgcgg cctcgggggc ggatatcgac
    43981 aaagttatct ttgacgctat gcatgcgctg gaagccaaac gtgaacaact gggtttaccg
    44041 tcctccgaca ctgaaatctc tgacacatgt cctccgtacg atgaagaagc ccgttctctg
    44101 gcggtggtca taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat
    44161 accttatcga catttaatgc cgatatgttg ctggaaaaaa acggcaaatg cgtcacacca
    44221 gagagtatta accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt
    44281 gcgaaagggc cagaagctga agaggcactg atcgctttcc gtcacctggc tgaagataac
    44341 tttggtgaaa cggaggaagt cgctccacct actctgcgtc ccgttctgcc tgtttcgggt
    44401 aaagcctttt attatcaacc agttttatgt acggtacagg caaaatcaac cctgaccgcg
    44461 gaagaagaac aagatcgatt acgccaggct attgacttca cgttattaga tctgatgacg
    44521 ttaacagcga aagcagaagc cagcgggctt gacgatattg ccgcaatctt ttctggtcac
    44581 catacactgt taggtgatcc ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa
    44641 cattgcacgg cagaatatgc ctggcagcaa gttcttaaag aacttagcca gcaataccag
    44701 caactggatg atgaatatct acaagctcgc tatattgatg tggacgatct tctgcatcgc
    44761 accctggtcc acctgaccca aacgaaagaa gaactcccgc agtttaactc gccaactatt
    44821 ctactggcgg agaacattta tccttccaca gtactgcaac tggatccggc ggttgtaaaa
    44881 ggtatctgcc ttagcgccgg aagtccggta tcccacagcg ccctaatcgc ccgtgaactg
    44941 gggattggct ggatttgcca gcagggtgag aaactgtatg cgatacaacc agaagaaacg
    45001 ctaacgctgg acgttaaaac gcaacgtttc aaccgtcagg gttaagccac cgttcggatg
    45061 gcatcgttct gatgtcatcc ggcaatttac ccagcactga tgttttttaa ctacctgtta
    45121 tattttttac caatttatga tgatttttct tcgctaatcg cgaaccaaat gtcgctggct
    45181 ggctaataaa cagacgttat ttgatcactc tatttcgccg ttatttatcc ctttaattcc
    45241 cttttctaaa atgcctgacc gttcgcagaa tgagatttcg atcatgcagc tagtgcgatc
    45301 ctgaactaag gttttctgat acttgaatac cgttttattc ggtttcgcca aaggagaatg
    45361 attgatgaaa tcccccgcac cttctcgccc gcaaaaaatg gcgttaattc cagcctgtat
    45421 ctttttgtgt ttcgctgcgc tatcggtgca ggcagaagaa acaccggtaa caccacagcc
    45481 gcctgatatt ttattagggc cgctgtttaa tgatgtgcaa aacgccaaac tttttccgga
    45541 ccaaaaaacc tttgccgatg ccgtgccgaa cagcgatccg ctgatgatcc ttgctgatta
    45601 tcggatgcag caaaaccaga gcggatttga tctgcgccat ttcgttaacg tcaatttcac
    45661 cctgccgaaa gaaggcgaga aatatgttcc gccagagggg cagtcactgc gcgaacatat
    45721 tgacggactt tggccggtat taacgcgttc taccgaaaac accgaaaaat gggattctct
    45781 gttaccgctg ccggaacctt atgtcgtgcc gggcggacgt tttcgcgagg tatattactg
    45841 ggacagttac ttcaccatgt taggacttgc cgaaagtggt cactgggata aagtcgcgga
    45901 tatggtggcc aattttgctc atgaaataaa cacttacggt catattccca acggcaaccg
    45961 cagttactat ttaagccgct cgcaaccgcc cttctttgcc ctgatggtag agttactggc
    46021 gcagcatgaa ggcgatgccg cgttgaagca atacctgccg caaatgcaaa aagaatatgc
    46081 ttactggatg gacggtgttg aaaacctgca agccggacaa caggaaaaac gcgttgtaaa
    46141 acttcaggat ggtacccttc tcaaccgcta ctgggacgat cgcgatacgc cacgaccaga
    46201 gtcatgggtg gaagatattg ccaccgccaa aagcaatccg aatcgacctg ccactgaaat
    46261 ttaccgcgac ctgcgctctg ccgctgcgtc tggctgggat ttcagctcgc gctggatgga
    46321 caacccgcag cagttaaata ccttacgcac caccagcatc gtaccggtcg atctgaacag
    46381 cctgatgttt aaaatggaaa aaatcctcgc ccgcgccagc aaagctgcag gagataacgc
    46441 gatggcaaac cagtacgaaa cgctggcgaa tgcccgtcaa aaagggatcg aaaaatacct
    46501 gtggaacgat caacaaggct ggtatgccga ttacgacctg aaaagtcata aagtgcgcaa
    46561 tcagttaacc gcggccgccc tgttcccgct gtacgtcaat gcggcagcga aagatcgcgc
    46621 caacaaaatg gcgacggcga cgaaaacaca tctgctgcaa cccggcggcc tgaacaccac
    46681 gtcggtgaaa agtgggcaac aatgggatgc gccaaatggc tgggcaccgt tacagtgggt
    46741 cgcgacagaa ggattacaaa actacgggca aaaagaggtg gcgatggaca ttagctggca
    46801 cttcctgacc aatgttcagc acacctatga ccgggagaaa aagctggtgg aaaaatatga
    46861 tgtcagcacc accggaacgg ggggcggcgg tggcgaatat ccattacagg atggctttgg
    46921 ctggaccaat ggcgtgacgc tgaaaatgct ggatttgatc tgcccgaaag agcaaccgtg
    46981 tgacaatgtt ccggcgacgc atccgaccgt taagtcagca acgacgcaac cctcaaccaa
    47041 agaggcacaa cccacacctt aaccagcgct tactccgaca acgtcattcg gcttctccgg
    47101 atggcgttgt cggcagataa caccatatct atggtttttt ctacacagac ggtttttcaa
    47161 gtcctggcgg gaagtattgc gcaaatgttt gccatacgtg gttgccgaag atagcgtcat
    47221 ttctacgggt aagtgtttca ctttcatcac ccggcgattt acgccgacgt tggctatata
    47281 aagcatcaat tgctaccgaa tattgttacg gtgttcactc atcaacgttt ttttgctttc
    47341 tggagttaaa ttaataatca cataatccag acactccaga gaatttagca accaaaaaaa
    47401 gaattcagac cattcctctt cgcgtaactg aagcattgcc gaacgggtat t
//
LOCUS       DBKELA010000018        47335 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.018, whole genome shotgun sequence.
ACCESSION   DBKELA010000018 DBKELA010000000
VERSION     DBKELA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.018"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="ACUQR0_001012"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="ACUQR0_001012"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate-cyanide
                     sulfurtransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HHV9108156.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            1144..1269
                     /gene="timP"
                     /locus_tag="ACUQR0_001013"
     CDS             1144..1269
                     /gene="timP"
                     /locus_tag="ACUQR0_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HHV9108157.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="ACUQR0_001014"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="ACUQR0_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HHV9108158.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="ACUQR0_001015"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="ACUQR0_001015"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HHV9108159.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="ACUQR0_001016"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="ACUQR0_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HHV9108160.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="ACUQR0_001017"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="ACUQR0_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HHV9108161.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="ACUQR0_001018"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="ACUQR0_001018"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HHV9108162.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="ACUQR0_001019"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="ACUQR0_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HHV9108163.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="ACUQR0_001020"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="ACUQR0_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HHV9108164.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="ACUQR0_001021"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="ACUQR0_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HHV9108165.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="ACUQR0_001022"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="ACUQR0_001022"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HHV9108166.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="ACUQR0_001023"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="ACUQR0_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HHV9108167.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="ACUQR0_001024"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="ACUQR0_001024"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HHV9108168.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="ACUQR0_001025"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="ACUQR0_001025"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HHV9108169.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="ACUQR0_001026"
     CDS             11233..12087
                     /locus_tag="ACUQR0_001026"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHV9108170.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="ACUQR0_001027"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="ACUQR0_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HHV9108171.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="ACUQR0_001028"
     CDS             complement(13550..14689)
                     /locus_tag="ACUQR0_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HHV9108172.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="ACUQR0_001029"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="ACUQR0_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HHV9108173.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /gene="hcaE"
                     /locus_tag="ACUQR0_001030"
     CDS             15875..17236
                     /gene="hcaE"
                     /locus_tag="ACUQR0_001030"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HHV9108174.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /gene="hcaF"
                     /locus_tag="ACUQR0_001031"
     CDS             17233..17751
                     /gene="hcaF"
                     /locus_tag="ACUQR0_001031"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HHV9108175.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="ACUQR0_001032"
     CDS             17751..18071
                     /locus_tag="ACUQR0_001032"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HHV9108176.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="ACUQR0_001033"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="ACUQR0_001033"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HHV9108177.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /gene="hcaD"
                     /locus_tag="ACUQR0_001034"
     CDS             18890..20092
                     /gene="hcaD"
                     /locus_tag="ACUQR0_001034"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HHV9108178.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="ACUQR0_001035"
     CDS             20189..20611
                     /locus_tag="ACUQR0_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HHV9108179.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="ACUQR0_001036"
     CDS             complement(20659..21531)
                     /locus_tag="ACUQR0_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HHV9108180.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTTLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="ACUQR0_001037"
     CDS             complement(21543..22637)
                     /locus_tag="ACUQR0_001037"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HHV9108181.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="ACUQR0_001038"
     CDS             complement(22670..23668)
                     /locus_tag="ACUQR0_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9108182.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="ACUQR0_001039"
     CDS             complement(23693..25204)
                     /locus_tag="ACUQR0_001039"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HHV9108183.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="ACUQR0_001040"
     CDS             complement(25227..26210)
                     /locus_tag="ACUQR0_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HHV9108184.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29588)
                     /locus_tag="ACUQR0_001041"
                     /pseudo
     CDS             complement(26307..29588)
                     /locus_tag="ACUQR0_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29706..30899
                     /locus_tag="ACUQR0_001042"
     CDS             29706..30899
                     /locus_tag="ACUQR0_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHV9108185.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="ACUQR0_001043"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="ACUQR0_001043"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HHV9108186.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="ACUQR0_001044"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="ACUQR0_001044"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HHV9108187.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="ACUQR0_001045"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="ACUQR0_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HHV9108188.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="ACUQR0_001046"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="ACUQR0_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HHV9108189.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="ACUQR0_001047"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="ACUQR0_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HHV9108190.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="ACUQR0_001048"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="ACUQR0_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HHV9108191.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="ACUQR0_001049"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="ACUQR0_001049"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HHV9108192.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="ACUQR0_001050"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="ACUQR0_001050"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HHV9108193.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44718)
                     /gene="tadA"
                     /locus_tag="ACUQR0_001051"
     CDS             complement(44215..44718)
                     /gene="tadA"
                     /locus_tag="ACUQR0_001051"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HHV9108194.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="ACUQR0_001052"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="ACUQR0_001052"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HHV9108195.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="ACUQR0_001053"
     CDS             45620..46468
                     /locus_tag="ACUQR0_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHV9108196.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47335)
                     /locus_tag="ACUQR0_001054"
                     /pseudo
     CDS             complement(46807..47335)
                     /locus_tag="ACUQR0_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11286 a        12962 c        11899 g        11188 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg tcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagttcaacg cattacgttg tagatcggta ttctggtcat ttgtt
//
LOCUS       DBKELA010000019        45795 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.019, whole genome shotgun sequence.
ACCESSION   DBKELA010000019 DBKELA010000000
VERSION     DBKELA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.019"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            114..2267
                     /locus_tag="ACUQR0_001055"
     CDS             114..2267
                     /locus_tag="ACUQR0_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HHV9108197.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="ACUQR0_001056"
     CDS             2320..4050
                     /locus_tag="ACUQR0_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHV9108198.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="ACUQR0_001057"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="ACUQR0_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HHV9108199.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="ACUQR0_001058"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="ACUQR0_001058"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HHV9108200.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="ACUQR0_001059"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="ACUQR0_001059"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HHV9108201.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="ACUQR0_001060"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="ACUQR0_001060"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HHV9108202.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="ACUQR0_001061"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="ACUQR0_001061"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HHV9108203.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="ACUQR0_001062"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="ACUQR0_001062"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HHV9108204.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="ACUQR0_001063"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="ACUQR0_001063"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HHV9108205.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="ACUQR0_001064"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="ACUQR0_001064"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HHV9108206.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="ACUQR0_001065"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="ACUQR0_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HHV9108207.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="ACUQR0_001066"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="ACUQR0_001066"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HHV9108208.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="ACUQR0_001067"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="ACUQR0_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HHV9108209.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribH"
                     /locus_tag="ACUQR0_001068"
     CDS             complement(14042..14512)
                     /gene="ribH"
                     /locus_tag="ACUQR0_001068"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HHV9108210.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="ACUQR0_001069"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="ACUQR0_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HHV9108211.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="ACUQR0_001070"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="ACUQR0_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HHV9108212.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="ACUQR0_001071"
     CDS             16308..16847
                     /locus_tag="ACUQR0_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HHV9108213.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="ACUQR0_001072"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="ACUQR0_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HHV9108214.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /gene="yajD"
                     /locus_tag="ACUQR0_001073"
     CDS             complement(18206..18553)
                     /gene="yajD"
                     /locus_tag="ACUQR0_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HHV9108215.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="ACUQR0_001074"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="ACUQR0_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HHV9108216.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="ACUQR0_001075"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="ACUQR0_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HHV9108217.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="ACUQR0_001076"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="ACUQR0_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HHV9108218.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="ACUQR0_001077"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="ACUQR0_001077"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HHV9108219.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="ACUQR0_001078"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="ACUQR0_001078"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HHV9108220.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="ACUQR0_001079"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="ACUQR0_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="ACUQR0_001080"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="ACUQR0_001080"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HHV9108221.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="ACUQR0_001081"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="ACUQR0_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HHV9108222.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="ACUQR0_001082"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="ACUQR0_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HHV9108223.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="ACUQR0_001083"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="ACUQR0_001083"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HHV9108224.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="ACUQR0_001084"
                     /pseudo
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="ACUQR0_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="ACUQR0_001085"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="ACUQR0_001085"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HHV9108225.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="ACUQR0_001086"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="ACUQR0_001086"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HHV9108226.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="ACUQR0_001087"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="ACUQR0_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HHV9108227.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="ACUQR0_001088"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="ACUQR0_001088"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HHV9108228.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="ACUQR0_001089"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="ACUQR0_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HHV9108229.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="ACUQR0_001090"
     CDS             complement(40011..40466)
                     /locus_tag="ACUQR0_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SF0329 family protein"
                     /protein_id="HHV9108230.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="ACUQR0_001091"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="ACUQR0_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="ACUQR0_001092"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="ACUQR0_001092"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HHV9108231.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="ACUQR0_001093"
     CDS             complement(41309..41986)
                     /locus_tag="ACUQR0_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HHV9108232.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="ACUQR0_001094"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="ACUQR0_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HHV9108233.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="ACUQR0_001095"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="ACUQR0_001095"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HHV9108234.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="ACUQR0_001096"
     CDS             complement(43192..43650)
                     /locus_tag="ACUQR0_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HHV9108235.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="ACUQR0_001097"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="ACUQR0_001097"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HHV9108236.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="ACUQR0_001098"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="ACUQR0_001098"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HHV9108237.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11907 a        11941 c        11203 g        10744 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 ggaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccaag gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacac cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc tagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat cagatggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atcgtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtggcg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat agtcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aagcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttatt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gagatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    45721 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac
    45781 tttcgtagct caccc
//
LOCUS       DBKELA010000020        43930 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.020, whole genome shotgun sequence.
ACCESSION   DBKELA010000020 DBKELA010000000
VERSION     DBKELA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.020"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(15..602)
                     /gene="mog"
                     /locus_tag="ACUQR0_001099"
     CDS             complement(15..602)
                     /gene="mog"
                     /locus_tag="ACUQR0_001099"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HHV9108238.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(717..1670)
                     /gene="tal"
                     /locus_tag="ACUQR0_001100"
     CDS             complement(717..1670)
                     /gene="tal"
                     /locus_tag="ACUQR0_001100"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HHV9108239.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1949..3378
                     /locus_tag="ACUQR0_001101"
                     /pseudo
     CDS             1949..3378
                     /locus_tag="ACUQR0_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            3448..4224
                     /gene="yaaA"
                     /locus_tag="ACUQR0_001102"
     CDS             3448..4224
                     /gene="yaaA"
                     /locus_tag="ACUQR0_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HHV9108240.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4377..4673)
                     /locus_tag="ACUQR0_001103"
     CDS             complement(4377..4673)
                     /locus_tag="ACUQR0_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HHV9108241.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="ACUQR0_001104"
     CDS             complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="ACUQR0_001104"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HHV9108242.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="ACUQR0_001105"
     CDS             complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="ACUQR0_001105"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HHV9108243.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="ACUQR0_001106"
     CDS             complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="ACUQR0_001106"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HHV9108244.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9596..9715)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="ACUQR0_001107"
     CDS             complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="ACUQR0_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HHV9108245.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9930..10616)
                     /locus_tag="ACUQR0_001108"
     CDS             complement(9930..10616)
                     /locus_tag="ACUQR0_001108"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HHV9108246.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11016..11156)
                     /gene="yjjY"
                     /locus_tag="ACUQR0_001109"
     CDS             complement(11016..11156)
                     /gene="yjjY"
                     /locus_tag="ACUQR0_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HHV9108247.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11252..11968
                     /gene="arcA"
                     /locus_tag="ACUQR0_001110"
     CDS             11252..11968
                     /gene="arcA"
                     /locus_tag="ACUQR0_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HHV9108248.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12027..13379)
                     /gene="creD"
                     /locus_tag="ACUQR0_001111"
     CDS             complement(12027..13379)
                     /gene="creD"
                     /locus_tag="ACUQR0_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HHV9108249.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13437..14861)
                     /gene="creC"
                     /locus_tag="ACUQR0_001112"
     CDS             complement(13437..14861)
                     /gene="creC"
                     /locus_tag="ACUQR0_001112"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HHV9108250.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14861..15550)
                     /gene="creB"
                     /locus_tag="ACUQR0_001113"
     CDS             complement(14861..15550)
                     /gene="creB"
                     /locus_tag="ACUQR0_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HHV9108251.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15563..16036)
                     /gene="creA"
                     /locus_tag="ACUQR0_001114"
     CDS             complement(15563..16036)
                     /gene="creA"
                     /locus_tag="ACUQR0_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HHV9108252.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16247..17116
                     /gene="robA"
                     /locus_tag="ACUQR0_001115"
     CDS             16247..17116
                     /gene="robA"
                     /locus_tag="ACUQR0_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HHV9108253.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="ACUQR0_001116"
     CDS             complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="ACUQR0_001116"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HHV9108254.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17812..18332
                     /gene="yjjX"
                     /locus_tag="ACUQR0_001117"
                     /pseudo
     CDS             17812..18332
                     /gene="yjjX"
                     /locus_tag="ACUQR0_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="ACUQR0_001118"
     CDS             complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="ACUQR0_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HHV9108255.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="ACUQR0_001119"
     CDS             complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="ACUQR0_001119"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HHV9108256.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20981..22648
                     /gene="ettA"
                     /locus_tag="ACUQR0_001120"
     CDS             20981..22648
                     /gene="ettA"
                     /locus_tag="ACUQR0_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HHV9108257.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22755..22922)
                     /gene="nadS"
                     /locus_tag="ACUQR0_001121"
     CDS             complement(22755..22922)
                     /gene="nadS"
                     /locus_tag="ACUQR0_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HHV9108258.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="ACUQR0_001122"
     CDS             complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="ACUQR0_001122"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HHV9108259.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24209..25591)
                     /gene="radA"
                     /locus_tag="ACUQR0_001123"
     CDS             complement(24209..25591)
                     /gene="radA"
                     /locus_tag="ACUQR0_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HHV9108260.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25640..26608)
                     /gene="serB"
                     /locus_tag="ACUQR0_001124"
     CDS             complement(25640..26608)
                     /gene="serB"
                     /locus_tag="ACUQR0_001124"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HHV9108261.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26714..27358
                     /locus_tag="ACUQR0_001125"
     CDS             26714..27358
                     /locus_tag="ACUQR0_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HHV9108262.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27386..28401
                     /gene="lplA"
                     /locus_tag="ACUQR0_001126"
                     /pseudo
     CDS             27386..28401
                     /gene="lplA"
                     /locus_tag="ACUQR0_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28433..28696
                     /locus_tag="ACUQR0_001127"
     CDS             28433..28696
                     /locus_tag="ACUQR0_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9108263.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28857..29576)
                     /gene="deoD"
                     /locus_tag="ACUQR0_001128"
     CDS             complement(28857..29576)
                     /gene="deoD"
                     /locus_tag="ACUQR0_001128"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HHV9108264.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29633..30856)
                     /gene="deoB"
                     /locus_tag="ACUQR0_001129"
     CDS             complement(29633..30856)
                     /gene="deoB"
                     /locus_tag="ACUQR0_001129"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHV9108265.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30908..32230)
                     /gene="deoA"
                     /locus_tag="ACUQR0_001130"
     CDS             complement(30908..32230)
                     /gene="deoA"
                     /locus_tag="ACUQR0_001130"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HHV9108266.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32357..33136)
                     /gene="deoC"
                     /locus_tag="ACUQR0_001131"
     CDS             complement(32357..33136)
                     /gene="deoC"
                     /locus_tag="ACUQR0_001131"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HHV9108267.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33394..34935
                     /locus_tag="ACUQR0_001132"
     CDS             33394..34935
                     /locus_tag="ACUQR0_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HHV9108268.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34907..35770
                     /locus_tag="ACUQR0_001133"
     CDS             34907..35770
                     /locus_tag="ACUQR0_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HHV9108269.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35984..36766)
                     /locus_tag="ACUQR0_001134"
     CDS             complement(35984..36766)
                     /locus_tag="ACUQR0_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9108270.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36763..37836)
                     /locus_tag="ACUQR0_001135"
     CDS             complement(36763..37836)
                     /locus_tag="ACUQR0_001135"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HHV9108271.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37958..38119)
                     /locus_tag="ACUQR0_001136"
     CDS             complement(37958..38119)
                     /locus_tag="ACUQR0_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HHV9108272.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38236..38841)
                     /gene="osmY"
                     /locus_tag="ACUQR0_001137"
     CDS             complement(38236..38841)
                     /gene="osmY"
                     /locus_tag="ACUQR0_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HHV9108273.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39234..40823)
                     /gene="prfC"
                     /locus_tag="ACUQR0_001138"
     CDS             complement(39234..40823)
                     /gene="prfC"
                     /locus_tag="ACUQR0_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HHV9108274.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40914..41591)
                     /gene="yjjG"
                     /locus_tag="ACUQR0_001139"
     CDS             complement(40914..41591)
                     /gene="yjjG"
                     /locus_tag="ACUQR0_001139"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HHV9108275.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41606..42052)
                     /gene="rimI"
                     /locus_tag="ACUQR0_001140"
     CDS             complement(41606..42052)
                     /gene="rimI"
                     /locus_tag="ACUQR0_001140"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HHV9108276.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42021..42434)
                     /gene="holD"
                     /locus_tag="ACUQR0_001141"
     CDS             complement(42021..42434)
                     /gene="holD"
                     /locus_tag="ACUQR0_001141"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HHV9108277.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42537..43568
                     /gene="rsmC"
                     /locus_tag="ACUQR0_001142"
     CDS             42537..43568
                     /gene="rsmC"
                     /locus_tag="ACUQR0_001142"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HHV9108278.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43845..43928
                     /locus_tag="ACUQR0_001143"
     tRNA            43845..43928
                     /locus_tag="ACUQR0_001143"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43879..43881,aa:Leu,seq:cag)
BASE COUNT        10338 a        12013 c        11120 g        10459 t
ORIGIN      
        1 tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca
       61 accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca gtacggtacg
      121 ctggcaaaaa taccatgcac cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc
      181 tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt gcgaatcacg
      241 cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt
      301 tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca
      361 cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc
      421 agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt
      481 tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgctggaat gcctttatcc
      541 tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc
      601 atcaagattt ttccgtttaa acaatctcgt taaacagaat gatacactgc gaagggagtg
      661 acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa gaatgattac
      721 agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt acggatacct
      781 tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa ctcggactca
      841 gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg ttcgatagcc
      901 ccttcgctct ccgccagctc tttcagcagt gccggtgcga tggtcagacg gtcacagcct
      961 gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac ggtttcataa
     1021 ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc
     1081 ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg gccaacaaac
     1141 ggcgagatca ggaacacgcc cgcctccgca caagcacgag cctgagcgaa ggagaacagc
     1201 agggtcaggt tacagttgat accttctttt tccagctgtt ctgcagcacg gataccctgc
     1261 caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc gttgtagagt
     1321 ttgatcaggc gttttgcttt cgcaattgac gcttcggtgt cataggaaag acgcgcatca
     1381 acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat atttactgcc
     1441 agtttgtcgg tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag
     1501 gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga
     1561 gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg
     1621 tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt
     1681 ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa
     1741 cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac
     1801 actgcgttga tatcgcccac catttttata caaaacctca tgtatgctac gcagaagtta
     1861 tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc
     1921 atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt
     1981 cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg
     2041 caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat
     2101 tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg
     2161 cgtgggtagc ggcaacctgg ccggcgttgc gctggcaatt accgccggtg gaccaggtgc
     2221 cgtcttttgg atgtgggttg ctgcgtttat cggcatggcg acctcgtttg ccgaatgttc
     2281 ccttgcacaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg gaccggcatg
     2341 gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct
     2401 catcgcctac ggcataattt tcagcggagt tcaggcgaac gccgttgccc gagccctgag
     2461 tttttctttt gattttcctc cgctggtgac gggcattatt ctcgctgtct ttgctctgct
     2521 ggcaatcact cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
     2581 ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg ggcaacttcc
     2641 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
     2701 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
     2761 tgaggtggga atgggatcga cgccaaacgc ggcagcggca gctgcatcct ggcctccgca
     2821 tccggcagca caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg
     2881 tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga
     2941 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctagg gtgctgaatt
     3001 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
     3061 cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
     3121 ctgcaccttc gcaacgatca tcggcggcac cttgctaagc cttccgctga tgtggcaact
     3181 ggcagatata taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
     3241 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
     3301 gtgtttgatc cgttgcgtta tccggatatc ggtcgccaac tttctccgga cgcgtgggat
     3361 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
     3421 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
     3481 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
     3541 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
     3601 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
     3661 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
     3721 caggccgaga ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
     3781 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
     3841 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
     3901 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
     3961 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
     4021 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
     4081 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
     4141 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
     4201 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
     4261 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
     4321 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
     4381 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
     4441 cgcggcggtg acggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
     4501 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
     4561 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
     4621 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
     4681 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
     4741 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
     4801 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
     4861 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
     4921 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
     4981 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
     5041 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
     5101 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
     5161 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
     5221 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
     5281 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
     5341 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
     5401 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
     5461 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
     5521 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
     5581 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
     5641 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
     5701 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
     5761 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
     5821 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
     5881 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
     5941 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
     6001 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
     6061 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
     6121 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
     6181 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
     6241 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
     6301 agagcgaaca aggtcgggcc ggagccggag ataccgctca ctaccgcgcc gatttccgcg
     6361 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
     6421 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
     6481 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
     6541 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
     6601 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
     6661 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
     6721 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
     6781 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
     6841 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
     6901 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
     6961 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
     7021 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
     7081 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
     7141 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
     7201 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
     7261 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
     7321 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
     7381 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
     7441 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
     7501 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
     7561 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
     7621 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
     7681 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
     7741 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
     7801 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtaacaa cgtggaaacc
     7861 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
     7921 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
     7981 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
     8041 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
     8101 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
     8161 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
     8221 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
     8281 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
     8341 acgcaaggtg cgcataccat cccctaccac cgagataatg gccagccgtt ccgtcaccgc
     8401 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
     8461 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
     8521 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
     8581 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
     8641 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
     8701 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
     8761 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
     8821 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
     8881 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
     8941 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
     9001 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
     9061 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
     9121 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
     9181 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
     9241 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
     9301 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
     9361 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
     9421 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
     9481 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
     9541 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
     9601 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
     9661 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
     9721 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
     9781 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
     9841 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
     9901 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
     9961 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
    10021 cagtcgacca gttttatgtc atctgccact gccagagccg tcagcaatgc catgactcgt
    10081 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
    10141 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    10201 taatccgcca ccatcggcac accagtaagc acgtcagcca acgctaactc ttcgttagtc
    10261 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    10321 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactacgc
    10381 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact
    10441 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
    10501 tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
    10561 gctgccccaa tattttctgc tctggcgggt gcgaccagga taatcgttat acgcatattg
    10621 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    10681 cgaatttaca aattcttaac gtaagccgca gaaaaagccc tttacttagc ttaaaaaagg
    10741 ctaaactatt ttctgactgc actaacggtt taattgttaa aaaatgctac atatccttct
    10801 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    10861 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    10921 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
    10981 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
    11041 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
    11101 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
    11161 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
    11221 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
    11281 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
    11341 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
    11401 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
    11461 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
    11521 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
    11581 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
    11641 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
    11701 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
    11761 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
    11821 cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
    11881 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accattcacg gtgaaggtta
    11941 tcgcttctgc ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aacggcgctt
    12001 tttagcgccg tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta
    12061 acttctttac tggctttcat tttcggcagt gaaaacgcat accagtctat attacgagtc
    12121 acaaacatca tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta
    12181 tcggcagagt tgagcagtcc ccacatcact ccatccagca acaacagcgc gaggataaac
    12241 aacatgctat tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc
    12301 ccaatcagac tggcgattat ccatgccacg gtaaaaccga tatgttcaga aagcgccagc
    12361 aagagcaaat aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa
    12421 cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa aagtcagtgc aatcagcaga
    12481 atggcgtact tagtcgcccg gtccgttaat tggtattgat cggctggcgt cgtcactgcg
    12541 acgctaaacg cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg
    12601 agattattag caaaccagct gctttgccat tgcgcctgaa aacctgactc gctagcttcc
    12661 cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc
    12721 atttcgctat tacgcccggc aggcaccaca gaaagatcgc cggtaccgct taaattcagt
    12781 gccatattca gctgcaggtt ctgcttacgc cagtcccctt cgggtaaagg gatatgcacg
    12841 ccctgcccgc cttgctctaa cccggtaccg ggttcaatgg tcagtgccgt tccgttaact
    12901 tcaggcgctt tcaccacacc aataccacgc gcatccccga cgctaatcac aataaagggc
    12961 ttgcctaaga tgatatttgg cgcgttgagc tcgctaagac gcgaaacatc gaaatcggct
    13021 tttagcgtta aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc
    13081 acgttctgat tgccatcaac catcaatgac tcaggtaaca aaaaatggat aaaacttcgt
    13141 ttccgctcca cggttttatc ctcttcctgc accgtataaa gctcggtcac aggaatagcg
    13201 ataagcggcc caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca
    13261 tcgctacggt aatcggcacg ttcgacaatc acttgccgaa tcagcattat cggaatcaac
    13321 aacagcaata ctgcaccaaa caaggtggtc attttccaga acagggggga tttcaacata
    13381 gtcttctcct ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat
    13441 gtgaagtgac ggtgaagtcg tagcgaggcc agcacgccac cttcctgcac gttgcgcagc
    13501 gtgacttcgc cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg
    13561 cttttttgcc catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg
    13621 tagtcaggaa taccactacc ggtatccagc accttaagcg tgacgtgttc ctgatccact
    13681 tcggcgctta gcgttatgca accgctctcg ggagtaaaat cgatggcgtt atccagtaaa
    13741 ttccccagcg cctgatccag taacgccggt tcagcagcaa cattaacctc ggtgggcata
    13801 acatgcaaag tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga
    13861 aataatgccg ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc
    13921 tggcgtagta acgtttccac cagcgcctgc attcgcgcat tttgtgtcag aatgttgtca
    13981 gtaaaacgag ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga
    14041 atcgccgcca gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag
    14101 tttttccctt ccagcttcac gcgcatactt tccagcgcct gcgcgagttt acgcagctcg
    14161 ctactaccga gatcgggaag aggaacgggc ttattgtcag tgacggaatc agcatagcga
    14221 gtgagccggg caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgca
    14281 atccccaaca aaatggcgct ggcccataaa attcgccgct cgctgcgttt aatgaccgga
    14341 gccatcgccg cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgcccata
    14401 atcggcgcgg caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg
    14461 cgcgcaccat actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgt
    14521 ccaacggctt tatttgccga atcgaacaat actttgccat gcgcatcggt catatagaca
    14581 tgatattcat tgcgcacttt gttaatgcca ccaatattgg cgcgaaacgg gcgatgttgt
    14641 agctgattaa acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga
    14701 cgcgccagct ccgcaagtaa cgttgccgtg tcgatcaacg tcccctccgt tgctcttcgc
    14761 acgcccggtt taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc
    14821 agtaaaaaat agcccagcag caaccgcatg ccgatacgca ttacaagccc ctcaggctat
    14881 atcccatgcc gcgatgagta ttaatcggtg aaagatcggg gttgatggcg cgcagcttgg
    14941 cacgcagcgt tttaatgtgg atatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc
    15001 acacgctatc cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct
    15061 tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg
    15121 ccgcgggttc attcaattca aaatgaccaa tacggatgac gggagacggc gtcgagaact
    15181 tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt
    15241 tagccacata gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc
    15301 gggccgtcag gaacagtaca ggtaacgccg gatggagcgc cagtaactgg cggcacaatt
    15361 caaagccact gatatcaggc aaaccaacgt cgagaatcat gacgtcggga acctgctggc
    15421 gagctttatc cagcaccggc aaacctcgct caaagacctc gacggcaaaa ccttcctgct
    15481 gcaacatgta gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt
    15541 cccgttgcat aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg
    15601 cgtttttcgg cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc
    15661 gtttggcgtc ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt
    15721 tgaatactac ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc
    15781 cgacttgctg acaagaaatg gccgcatcgg aggtatcttc tgccaggccc aatccccctt
    15841 taataccacc ggttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt
    15901 caaaggcttc cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg
    15961 aaccaatctc ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca
    16021 aatgcttgta tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac
    16081 ggtaaataac atattgatta agaacaaaat ctcaattctt ttatttccgt caagccctaa
    16141 aacatactct actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca
    16201 attacctgat gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg
    16261 cattattcgc gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga
    16321 caatgtagcg gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt
    16381 cactggccat gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc
    16441 actacgcctg actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca
    16501 acagacattt acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg
    16561 ttctcctgaa tggagcgcct ttggtattcg cccgccgcta cgtctgggtg aattcacaat
    16621 gccagagcac aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta
    16681 ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca
    16741 cgattttctc ggcaacgcgc cgaccattcc gccggtgctc tacggcctga atgaaacgcg
    16801 tccgagtcag gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga
    16861 tcaggcagat ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt
    16921 gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg
    16981 aacgtgcatg ccaatgctca atctgacgcg ccgtaaaggt caggatattg agcgatacta
    17041 cccggcagaa gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat
    17101 tcctatccgc cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc
    17161 ccctgcggtt tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg
    17221 cgaaatagaa cagttacgca gacgtaagcg gcgctctacc catgctggta atccgagaat
    17281 cgtactcacc agacatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg
    17341 cggtaagtcc cggcaagatt ccagtgccgc attgacgcga tcgctgagct cctgcattga
    17401 ctcgccttca ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc
    17461 ttcttcttcg gtcagagaat cgatatgtct tttttccagc acacccatgt ttaattcacg
    17521 caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg
    17581 ccgggtgcgt cctaaatcgc tactgataat atgcgtaatg ccaagctctt tggcacgggt
    17641 tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg
    17701 aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg actaggtata cctgtaacat
    17761 gctttttttc cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa
    17821 gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag
    17881 atcttcggcg aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa
    17941 caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc
    18001 cgtttacttc cagaggctga tttttgggtg gcgattgaag ctggcattga tggcgacagc
    18061 actttcagtt gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg ttcggcaact
    18121 ttgccgttac ctgcggtgat tctggaaaaa gttcgtgaag gcgaggcgct tggtcccgtg
    18181 agtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta
    18241 ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc
    18301 cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg
    18361 acattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa
    18421 tcgcttttca gcaacacctc ttccagccac tgacgcagct ccacgggcgc ggctttcagg
    18481 ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc
    18541 tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg
    18601 cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca
    18661 ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct
    18721 gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgac
    18781 tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac
    18841 gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt
    18901 aagcatcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct
    18961 cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc
    19021 caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa
    19081 catattgcag gtaactggtg ccaatgttga tgttcgtttc cggatccagc aattgcccag
    19141 ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa
    19201 tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct
    19261 gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga
    19321 aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga
    19381 tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag
    19441 cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtattat
    19501 cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc
    19561 tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcaccg atgcgttgtg
    19621 ctgcaaccat cgggtagaaa ccacgctgtt gtatgagttg atgcaaaatc tctttcgctt
    19681 cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat tcatctttct
    19741 cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc
    19801 caagcgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt
    19861 catcgcgcca tttcgcctgc tcatcggtga catcattgcc catcaaacgc caggcgacga
    19921 tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cgcctgggca agcgacggga
    19981 tcatcagccg tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc accgccgcca
    20041 tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt
    20101 tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg
    20161 ccagcactgt taccaggcct gtgttacccg ctttcatcgc cagacggata cgctctaaat
    20221 acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt
    20281 taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct
    20341 gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt
    20401 ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac
    20461 gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg
    20521 tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg gtattccagg tagggataaa
    20581 gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatctgt cgattatccc
    20641 aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca
    20701 cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg
    20761 ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa
    20821 tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt
    20881 actgcgaagg tgatcgacca actgggatta ggcggggaaa caggctacac ttcgcctttg
    20941 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    21001 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    21061 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac
    21121 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    21181 agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac acaccgtgcg
    21241 tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt
    21301 gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    21361 tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc
    21421 ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc gctaacctct ccggtggtga
    21481 gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    21541 cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga
    21601 cttcgaaggc accgtggtgg cgattactca cgaccgttac ttcctcgata acgttgcagg
    21661 ctggatcctc gaactggacc gcggtgaagg tattccgtgg gaaggtaact actcctcctg
    21721 gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    21781 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg
    21841 taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaataccaga aacgtaacga
    21901 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    21961 caacctgcgt aaatcctacg gcgatcgcct gctgattgat gacctgagct tctcgatccc
    22021 gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg
    22081 tatgatctcc ggtcaggaac agccggacag cggcaccatc actttgggtg aaacggtgaa
    22141 actggcatcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    22201 agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta
    22261 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgtgttggcg aactttccgg
    22321 tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    22381 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    22441 gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    22501 cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt
    22561 tactgagtac gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg
    22621 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct
    22681 tatccggcct acaaaaccag caatttcaat acgttgtaaa actgtaggcc tgataagacg
    22741 cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tgcggcgtga acaccttttc
    22801 cggtctacaa cagccttagc atcctcaatt tcgtggcggg tcacccgtgc aggttttttc
    22861 aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc
    22921 attttatacc tcgcctttga gcagtttcgt cacggttatc cttgctcccc catcatctcc
    22981 cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg
    23041 aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc
    23101 accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg
    23161 atcaccagat cgaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcacgccct
    23221 tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta
    23281 ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtggc ccaacgcgat tttatcgtag
    23341 tcggaatact gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata atcgcggcca
    23401 tattcccacg cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta
    23461 ccgctcgact cgccgccaag gatcgccacg gtacgcacaa aaaacggctt cacttcggta
    23521 ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata
    23581 aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca gatgttccat atactgtggc
    23641 gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc agccataaac
    23701 tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat gccctcttcg
    23761 ttgaaagcat gaatacggat atttttctga tatttaaagg tttgcaatag ccagcgcaga
    23821 cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg
    23881 gtatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata
    23941 aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc
    24001 ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg
    24061 gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca
    24121 tcagctacct gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat
    24181 gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg
    24241 agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg
    24301 ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac
    24361 tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg
    24421 gcagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcgccgctgg
    24481 tttcggttac cttcacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca
    24541 gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca
    24601 ctgccacgcg gcgcggattc gccattatcg agtgatcgac cagcgcctga atctccacca
    24661 gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc
    24721 cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg
    24781 cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgt aaggtgcgaa
    24841 aacgggagtc ggcatcgcca tccagaagca ccgaacagtc gatacagtgt tccagcactt
    24901 tcgggccagc cagcgaacca tctttggtta cgtgtccaac catgacaatc gccacaccgc
    24961 gcgttttggc gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag
    25021 gcgatgattg tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg
    25081 gttgctcttc ttcggcaatc aggcagatct gctcgatgct ggtttccgac aacatattga
    25141 gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt
    25201 cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagcaaca
    25261 gcgtggattt ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc
    25321 cgcctcctag tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa
    25381 ggctgatatc ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga
    25441 gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc
    25501 aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac
    25561 aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg
    25621 caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg cagaataccc
    25681 ccatcaggtc agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat
    25741 ggtaggcaat ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa
    25801 tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg
    25861 ctttgtactg cgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga
    25921 tctccagttc attggctacc acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa
    25981 agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca
    26041 ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg
    26101 cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc
    26161 cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc agcttggcaa
    26221 tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg
    26281 gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc cagctgcgct tcgtgggcca
    26341 ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc
    26401 accaggcggc aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct
    26461 gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg
    26521 gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag
    26581 gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga
    26641 aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt
    26701 caaagggtgg aagatggctc gcacaaaact gaaattccgg ctacatcggg cagtgattgt
    26761 cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa
    26821 ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt
    26881 gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc
    26941 gatcctcgat cagttaacag atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca
    27001 aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga
    27061 cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa
    27121 cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca
    27181 acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg
    27241 cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt
    27301 cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc
    27361 cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta
    27421 cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca
    27481 gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc
    27541 gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag
    27601 tggcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg
    27661 caagccggag tacgataaaa ctctctccac gtcgattgtg ctcaatgcgc tgaacgcgct
    27721 cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga
    27781 ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc accacggcac
    27841 cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa
    27901 actggcggcg aaaggcatta cttcagtgcg ttcccgcgtg accaacctca ccgagctgct
    27961 gccggggctc accatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
    28021 ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc
    28081 gccgaaacct ttgcccgtca gagtagctga gagtggaact tcggtcaggc tccggcattc
    28141 tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa
    28201 aaaggccata tcacccgcgc ccaggtattt accgacagcc tcaacctcgc gccgctggaa
    28261 gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc
    28321 gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg
    28381 atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa atatgatcga
    28441 tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta tttcctggct
    28501 agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat
    28561 ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa acatcaaaag
    28621 caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac
    28681 ggctgaacat aaatgaatag attcgtgaat agatacttga atgtttttta ttttctatcc
    28741 atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag
    28801 ccggagcagt cccccactcc ggcttaacac gacaatcgcc tttcagcgaa acacaattac
    28861 tctttatcgc ccagcagaac ggattccagt gcgattttga tcatgtcgtt gaaggtggtc
    28921 tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac ggtgcagatg
    28981 gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga taccagccgc ttccatttcc
    29041 acgccgagga tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac
    29101 aggtcagcgg agaacaggtt acccacgcga gcatcaacgc ccagtgcttt agctgcatct
    29161 actgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatggtc tttaaaacgg
    29221 atgcggttaa ctttggaatc ggtgcaggcc cccataccga taacgacgtc gcgcagtttt
    29281 acgtgcggca gaactgcgcc acaggaacca acgcggataa ttttcttcac gccgaaatcg
    29341 gtgatcagtt ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg
    29401 gaaattttgc ggcctttgta agtaccagtg aagcccagca taccgcgaac gttgttcact
    29461 tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc
    29521 atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt
    29581 ttatcctttt tgtgacataa caaaggcgga gtcaacgctc cgcccaaatc catcagaaca
    29641 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    29701 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggcca tataccagta
    29761 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcgcaa ccgtggtcag
    29821 cggtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    29881 cgaacagttc cagacccgca gcataaccgg cgacgtcgcg gcggtggccc caggaagagt
    29941 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    30001 tggtggcgtc aaacagcgcg tccaagccag tcgctttcac ttttttggtg ataccgcagt
    30061 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg gccgtgtttt tcatcaacca
    30121 gtttctgcag cacggtcggt gctggcggct caacagccaa gtcgtgacgg ttaccggtac
    30181 gctggaagtt accagctttg tcaccgataa acggacgagc gataacacga ccgatattgt
    30241 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccagac
    30301 cgaaagtttc ttcatggcag gcaatctgga acacggagtc agcggaggta tagaaaatcg
    30361 gcttgccggt tttcatgtgc tcttcgccca gttgatccag aatgaccgta ccggaagagt
    30421 ggcagttacc gaggtaaccc ggcagattag cgcgttcgac cagtttatcc agcagctctt
    30481 gcgggaagct gttttcgtga tcggagaaat atccccactc aaacagaacc gggacaccgg
    30541 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    30601 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    30661 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcggc cctttacgac
    30721 cattatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    30781 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    30841 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    30901 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gcgttatcgg
    30961 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    31021 cgaggataac cgccagcgga cgctgaccgt ctacctggtc gcccagacgc gccatatcag
    31081 taaagccgac gctgtaatcg atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    31141 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    31201 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    31261 tcgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta ccgttgtcca
    31321 gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagatca
    31381 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    31441 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    31501 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    31561 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    31621 tcacgtccat caccagcgcg tccagacctt ccgccagttt cttcgccagg atcgaggcag
    31681 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    31741 cagccggagc cagtgaactg gtctgaccga taatcgccac gccgacgtct ttaataattt
    31801 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    31861 gcgtaccgcc agtatgaccg aggccgcgac cggagatcat cggaatatag ccgccgcagg
    31921 ctgcgaccat cggccccaac atcagcgaag tgacatcgcc gacgccgccg gtcgagtgtt
    31981 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    32041 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    32101 tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc gcgaataccg ttgataaaga
    32161 aacgaatttc ttcatcgctc agcgcatgac catcacgttt tttacgaata atttcttgtg
    32221 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    32281 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    32341 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcgccgtgg cccagcgctt
    32401 tcagcaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tcagcccagt
    32461 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    32521 cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    32581 tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    32641 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    32701 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    32761 tacaggcttt caccaggtca aaaccaacct gctcgtcacc cgccatcagc gcgcggtacg
    32821 ggaacaccac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    32881 cgatttcgat gtcgtcgtta ccgtgtggga agttggttac cgtagcgata cggatttccg
    32941 gggtgccctg ctctttcaga gttttgcgag caatcgggat aaaacgagga tagatacaga
    33001 tagcggcggt attgccgacc ggagttttgg cctgatgaca cagagcaatt actttctcgt
    33061 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    33121 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    33181 tgttagtatt ctaacatcta ctccgcaaca cacttcgata cacatcacaa ttaaggaaat
    33241 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    33301 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    33361 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    33421 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    33481 tttctggcac tggaagcaga aaacaatctg ccttacccac agcttcctgc cgaagcccgc
    33541 cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    33601 cgctatgtct tacccgatta cgctcgtttt ctggcgaacg gttccgaatg gctggagctg
    33661 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    33721 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccgtat
    33781 gttagaattc taacacaaaa cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    33841 gacagaaccc tgccagatgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    33901 acccgcgcga tcttacgtgc agacgcgcag gtttcaccga acctgacctt tatctacgat
    33961 cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg tgaatgtagc
    34021 aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gggctacggg
    34081 attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt
    34141 aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta
    34201 ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa
    34261 caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt
    34321 tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtgagaaa
    34381 gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg ttatcgcatc
    34441 agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc
    34501 gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc gggcgcgcgt
    34561 ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat
    34621 gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaac
    34681 ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttacc
    34741 gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat
    34801 ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg cgaagaagcc
    34861 gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg
    34921 cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc
    34981 tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg
    35041 acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt
    35101 tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct gcctgaagat
    35161 gtgtccgcaa catgccacac caatggcgca atccatgagc gtggacgaag tgcttagcca
    35221 tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg aagccacgac
    35281 ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca
    35341 tctcacctgt ctggtggaca gtaacggtat gttgagcgaa accggctggg aaaaattact
    35401 cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat gtcatcaaca
    35461 attaaccgga cgcgataatc aacagattaa gcgcagcatc tatttgctgg cagagcgcgg
    35521 caagctggcg gaactgcgtt tgctgatgat cccaggccag gtggattatt tgcaacacat
    35581 cgaagaactg gcggcgttta tcaagggact tggtgatgtt ccggttcgcc tgaacgcgtt
    35641 tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt
    35701 tgagccgttg gctgatgcat taaaggtgcg cggggtgagc cggttgatat ttccggcgct
    35761 ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg cctgataaga
    35821 cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta
    35881 tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgttaag cgtcgcatca
    35941 ggcattatgc gccgactgcc ggatgctgcc tacgaagggc aaactaccca cgaacgttaa
    36001 acaccgcata cgtgttatta agcaacactt cggcaatctc atccgcgggt tccgggcgta
    36061 actcgcaaag cacggcaaac acacgggcag cctgttccgg gcggttaggc tgcccctgaa
    36121 aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa caacgatgcc agcggtaatt
    36181 ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg cctacgccga
    36241 ttttgtagcc cagctgtaca aaccgttcag cctgttgctg gctgccagaa aatccgtgga
    36301 caacaccagc acgcggcaaa tcgtggtgtt taagatgcat cgccagtttg tcgtgcgtgc
    36361 gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc cagtttcagt tgttcgtcga
    36421 gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga ccgatctccc
    36481 ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctgc tcaagagaca
    36541 catcgctatg tttttccaac ataccaggat gcaagcccaa tgcggcatac agcggttgat
    36601 aattttccgc taacgcctgc acgcgggcaa aattttccgc ctctgtcgcc ggaacaatga
    36661 tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg ccaataaaag
    36721 gcgggaaatc aaaatggcag tgagtgtcga taaaacggca aatcaagcca gatcctcatt
    36781 gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact tcggcaacca gcgtatcgtt
    36841 ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc accactggca ccagatggcg
    36901 ggtaagcggc gctttttcag ttaatagctt gccaaccgtg gcgaggaaat aacgaccgca
    36961 taaacgccca agtttatagt cttcacgcag cgccggaatc cgactgccaa gcgcgatact
    37021 atgtaatggc ttcggcggat aaatttcgga tatccgcagc ttgcccggtg gtttctcaat
    37081 aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga ccagcggctg
    37141 caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg tgtagtacat
    37201 ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg ccgcctcttt
    37261 aaccggaatc gcatcactga tcccgccatc caggtagtta atgccttcca gcgacactcc
    37321 gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc agttttgttt
    37381 ggttggtaaa aagtaattcg gctcgtagtc atcctgacga caggcgcaca tataaaacga
    37441 tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct ggcttgcggt
    37501 ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc gcaatggatc
    37561 gaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac cgggctgatt
    37621 gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa gatcgaaagg
    37681 attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc cacgctgtcc
    37741 gccgccttcg cacaccagag ctattcgtcc aggctggaac ggccttagcg acaacggcgc
    37801 aatattacca agcgttacag gtattcgctg ccccacttct gccttcctgt ttttgttgtg
    37861 aaagaaagac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc agtccgctgg
    37921 actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt cggcccatga
    37981 acaaactcac taggaacaga ataatcccga cgacaaagac aattttagct gcgcctgcag
    38041 cggtaccggc cagaccacca aacccaagtg cggcggcgat taacgcgata accagaaata
    38101 tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc gcttgatatg
    38161 agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc tcccgacaaa
    38221 gttcgggagg acgaattact tagttttcag atcattttta acgcttttca caccatctac
    38281 cgctttggcg atactttcag cacggtcact ttgtgcctga gaatctacgg taccggagag
    38341 ctgaaccacg ccatcggtgg tttcaacttt cactttacgt gaagggacga tatcgtccgc
    38401 cagcagtttg gctttgattt cactggtggt ggcggtgtca cccgcgtagc ccttcaccga
    38461 gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca gaggtcacgc cttcaacgcc
    38521 tttcgccact ttcactgcct cttcggcctg ggcctgactt tcaacgaaac cgctcagggt
    38581 cacgactttt tgatcggttt tgacagagat atcggtgctc ttgatgttgt catgatccac
    38641 cagcgccgcc ttcactttcg cggtgatggc gctgtcatcc atgaaattac cgactttatt
    38701 catagagcta tcgacttttt gccctgcgct ttcattggta gtctgcgcgt tgttttccgc
    38761 gtaggcagag ccggtcgcga cggcagaggt caacattaca gccagcagag ttttcgaaat
    38821 cttcagtctt gtcatagtca tcgatttatt cctgtatgtt tgctcgtaat ttgagcctag
    38881 caacacgagg ttgcattgct gaatagggag agacttcacc ctctacagaa gtcaatggtc
    38941 gccatcacaa aagcgatggg tgatgaataa cgaccattac agcctctgaa tcagttatta
    39001 atatcggtag aatgacaatc gacggcttta gatactgata tctacgcatt gaacggtatt
    39061 taacgccatc agaaatgtca tcactttgtt aaatatagat cacaattttg aaaccgctcg
    39121 ggatatcagc gaaaacataa gcaaaagtga atgttttaag aacattccgt aagcggctaa
    39181 taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc ctggaggcaa gaattaatgc
    39241 tcgcgggtct ggtggaactg aacgtccgga taacgttcct gtgccaggcg caggttgacc
    39301 atgctggtag cgatgtaagc gaggttatcg ccgccatcaa gcgccagttg gctttcgttc
    39361 ttacgcttga actcttcgaa tttcttcgcg tctgcacatt ctacccagcg ggcagtggca
    39421 acgttgactg actcatacac tgcttcaacg ttgtattcgc tcttcaggcg cgctaccacc
    39481 acatcaaact gcagcacacc aaccgcacca acgatcagat cgttgttgga gattggacgg
    39541 aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc ctttgagcag ctgtttttgc
    39601 ttcagcggat ctttcaggcg gatacgacgg aacagttctg gtgcgaagtt cggaataccg
    39661 gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga tctgaatggt gccgtggttg
    39721 tgcaggccga ggatatcgcc cggatacgct tcttcaacgt gcgaacggtc acccgccata
    39781 aaggtcagcg cgtcggagat caccacatct ttcgcagtgc gcacctggcg cagtttcatg
    39841 cctttttcat atttaccgga caccacacgc ataaacgcca cgcggtcgcg gtgtttcgga
    39901 tccatgttgg cctgaatttt aaatacgaag ccggtaaatt tatcttcgct cgcttctacg
    39961 gtacgggtat cggtctgacg cggcatcggc gcaggcgccc actccaccag gccattcagc
    40021 atatgatcaa cgccgaagtt acccagcgca gtaccgaaga ataccggagt gatttcgccc
    40081 gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt taaccagttc cagttcgtca
    40141 cgcagctgct gtgccagatc ttcaccaacc gcagcatcga gatccgggtt attcagccct
    40201 ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc tctgatagag ataggtttca
    40261 tctttataaa ggtggtaaac gcctttaaac agcttgccac agccaattgg ccaagtgatc
    40321 ggtgcgcagc caattttcag ctcgttctca acttcatcga gcagttccat cgggtcgcgg
    40381 atatcacggt caagtttgtt cataaaggtg aggatcggcg tgtcgcgcag acgggtaact
    40441 tccatcagct tacgggtacg atcttcaaca ccttttgcgg cgtcgataac catcaggcag
    40501 cagtccaccg ccgtcagggt acgataggta tcttccgaga agtcttcgtg ccccggggtg
    40561 tcgagcaggt taaccaggca atcgtgatac ggaaactgca tcacagacgt agtaatggag
    40621 atcccacgct gcttttccat ctccatccag tccgacttag cgtgctggtt ggaaccacgg
    40681 ccttttactg taccggcggt ctgaatggcc tgtccgaaca gcagcacctt ctcggtgatg
    40741 gtagttttac cggcgtccgg gtgagaaata atggcaaaag tgcggcgctt cgccacctct
    40801 ttcaaataag gagacaacgt cataattctt tcttcttgag taagcggcat cgcgccgcgc
    40861 ttgttgaaaa cgaaaaattg cggctatttt acccatcaac gggggggagg caatcagtgt
    40921 ttacacagga gctgctccag ttcgtgcaac gaagaaacgg tccaggtggg cgcgatgcct
    40981 tctggttgct cgcgattgtg cgcattcagc cagcaggtcg caagcccggc gttgatgcca
    41041 ccgagaatat cggactcggc agtgtcgcca accatcagca cgcgtgaacg gtcaggattg
    41101 cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg gtttggcaac gccaacttct
    41161 tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg tccgttccag acgcacttgc
    41221 tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag cgttgctacg aatggcgtta
    41281 agcagagaaa ccgcgcccgg caacggcgta cagatttccg ccatcgcatt aataaaggca
    41341 tcgttaagca agcctgcttc aactttcagc cgttcggccc agctctcgaa acgcccgtgc
    41401 tgaagctgta atgaagtgat cgcgccgttt tgataatcca cccacagtgg cttgttaacg
    41461 gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc tgtaatcaag aaacatccgc
    41521 tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat cggcatcaaa gaaaatccag
    41581 tcccacttca ttattccacc ttgtcttaca tactgattgg caacgccatg atgatggcgt
    41641 cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat cgtcgcctcg ttaaagccta
    41701 aactttcgta cagggcaatg gcggcagcat ttgaggcacg gacttccagc catagtgtcg
    41761 ccacgccgcg tttttccagt tcgtcgatca gatgttccag cagcgcccta cccagcccct
    41821 gacgctgata gtcgggatcg accgcaatat tgaacaatgt agcttcatcc agcaccactt
    41881 gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa ctgaaagttg agataacgct
    41941 cgccctggtt gctggcaaag gttttttcac tccacggaaa ggagtgggcg cgttgttcaa
    42001 tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga aatcgtgttc atatgtgcaa
    42061 atttgttgcc ataacgcggc gcgtgccgtt gggtttgccc gtaattcggt gagcgccggt
    42121 gatgccacct gagcgccttc caatgatagc ggttcgtcag tacccaaccg ccagctgttg
    42181 cagcgactgc cttgcggcag catcgcgatt ttttctggcg tcagttgcag cacttgatcg
    42241 gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag gatcagtcag ggcgggaaga
    42301 tcgtttgcca ccatcaccag acggacgtgt gccgggctcg caatggcaat ctccccctgc
    42361 aacgcgccag ggcgacgcag cgaccactgg gtaatgccca gttgctgtaa ctgccagtct
    42421 cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc gcaaatatag caaattcgtc
    42481 gataccgcgc caacatatgg ctataatcgc cgccagtatc aattgaggag cattccatgt
    42541 ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag tgatgatttc gaacaaagcc
    42601 gtattctgtt tgccggagac ttacaggatg acctgcccgc gcgtttagat accgcggcca
    42661 gccgtgctca tacccagcaa ttccaccatt ggcaggtgtt aagctgccag atgggggata
    42721 acgcccgttt tagtctggtc gccacggcga atgatgtcgc agattgcgat acgctgattt
    42781 actactggcc gaagaacaaa ccggaagccc agttccagtt gatgaattta ctttctctgc
    42841 tgccagtggg gacagatatt tttgtcgttg gcgagaaccg cagcggcgtg cgcagcgctg
    42901 agcagatgct ggcagattat gcgccgttga ataaagtcga cagcgctcgt cgctgtggcc
    42961 tctattttgg tcgtctggaa aaacagccag tatttgatgc ggataaattc tggggcgaat
    43021 acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt gtttagccgc gacggtctgg
    43081 atgtcggtag ccagttgctg ctctcgacgt taactccgca cacgaaaggt aaagtgctgg
    43141 atgtcggctg tggcgcgggt gtactttcag ttgcctttgc gcgccattcg ccgaaaattc
    43201 gtctcacctt gtgcgatgtc tctgccccgg cggtagaagc cagccgcgca acacttgcag
    43261 ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt ttccgaggtg aaaggttgtt
    43321 ttgatatgat catctccaac ccgccgttcc acgatgggat gcaaaccagc ctggatgcgg
    43381 cgcaaacgct gattcgtggc gcggtgcgtc atcttaatag cggcggcgag ctgcgaattg
    43441 tagcgaacgc cttcctgcct tacccggacg tgctggatga gacatttggc ttccatgaag
    43501 ttatcgcgca aaccgggcgc ttcaaggtat atcgcgccat tatgacccgc caggcgaaga
    43561 aaggttgagt atctgatatc tgtcgccgca taccgtgtcg gatgcggcgt gaacgcctta
    43621 tccgacctac aaaacctgca cgttagccct tcgtaggcca gataagacgc gccagcgtcg
    43681 catctggcat ctgcccccgc atgcctacca aacctgcacg ctagtttcct gatggacatt
    43741 tttccagcaa ttacacatca ggcgataatt aactattgac gaaaagctga aaaccactag
    43801 aatgcgcctc cgtggtagca attcttttta agaattgatg gtatgcgaag gtggcggaat
    43861 tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc
    43921 ccctcgcacc
//
LOCUS       DBKELA010000021        43481 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.021, whole genome shotgun sequence.
ACCESSION   DBKELA010000021 DBKELA010000000
VERSION     DBKELA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.021"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            238..1671
                     /gene="cscA"
                     /locus_tag="ACUQR0_001144"
     CDS             238..1671
                     /gene="cscA"
                     /locus_tag="ACUQR0_001144"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HHV9108279.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1679..2674)
                     /locus_tag="ACUQR0_001145"
     CDS             complement(1679..2674)
                     /locus_tag="ACUQR0_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHV9108280.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2905..3123
                     /locus_tag="ACUQR0_001146"
                     /pseudo
     CDS             2905..3123
                     /locus_tag="ACUQR0_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3141..4469
                     /gene="dsdA"
                     /locus_tag="ACUQR0_001147"
     CDS             3141..4469
                     /gene="dsdA"
                     /locus_tag="ACUQR0_001147"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HHV9108281.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4577..6115)
                     /gene="emrY"
                     /locus_tag="ACUQR0_001148"
     CDS             complement(4577..6115)
                     /gene="emrY"
                     /locus_tag="ACUQR0_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HHV9108282.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6115..7278)
                     /gene="emrK"
                     /locus_tag="ACUQR0_001149"
     CDS             complement(6115..7278)
                     /gene="emrK"
                     /locus_tag="ACUQR0_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HHV9108283.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7694..8308
                     /gene="evgA"
                     /locus_tag="ACUQR0_001150"
     CDS             7694..8308
                     /gene="evgA"
                     /locus_tag="ACUQR0_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HHV9108284.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8313..11906
                     /gene="evgS"
                     /locus_tag="ACUQR0_001151"
     CDS             8313..11906
                     /gene="evgS"
                     /locus_tag="ACUQR0_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HHV9108285.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11962..13107)
                     /gene="yfdE"
                     /locus_tag="ACUQR0_001152"
     CDS             complement(11962..13107)
                     /gene="yfdE"
                     /locus_tag="ACUQR0_001152"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HHV9108286.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13181..14125)
                     /gene="yfdV"
                     /locus_tag="ACUQR0_001153"
     CDS             complement(13181..14125)
                     /gene="yfdV"
                     /locus_tag="ACUQR0_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HHV9108287.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14195..15889)
                     /gene="oxc"
                     /locus_tag="ACUQR0_001154"
     CDS             complement(14195..15889)
                     /gene="oxc"
                     /locus_tag="ACUQR0_001154"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HHV9108288.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15943..17193)
                     /gene="frc"
                     /locus_tag="ACUQR0_001155"
     CDS             complement(15943..17193)
                     /gene="frc"
                     /locus_tag="ACUQR0_001155"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HHV9108289.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17706..18329)
                     /locus_tag="ACUQR0_001156"
     CDS             complement(17706..18329)
                     /locus_tag="ACUQR0_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HHV9108290.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18626..18901
                     /gene="ypdI"
                     /locus_tag="ACUQR0_001157"
     CDS             18626..18901
                     /gene="ypdI"
                     /locus_tag="ACUQR0_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HHV9108291.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18978..19220)
                     /locus_tag="ACUQR0_001158"
     CDS             complement(18978..19220)
                     /locus_tag="ACUQR0_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HHV9108292.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19573..20493
                     /gene="lpxP"
                     /locus_tag="ACUQR0_001159"
     CDS             19573..20493
                     /gene="lpxP"
                     /locus_tag="ACUQR0_001159"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HHV9108293.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20848..20919
                     /gene="ypdK"
                     /locus_tag="ACUQR0_001160"
     CDS             20848..20919
                     /gene="ypdK"
                     /locus_tag="ACUQR0_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HHV9108294.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20984..22222)
                     /gene="alaC"
                     /locus_tag="ACUQR0_001161"
     CDS             complement(20984..22222)
                     /gene="alaC"
                     /locus_tag="ACUQR0_001161"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HHV9108295.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22599..24085
                     /locus_tag="ACUQR0_001162"
                     /pseudo
     CDS             22599..24085
                     /locus_tag="ACUQR0_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            24100..24834
                     /gene="ypdB"
                     /locus_tag="ACUQR0_001163"
     CDS             24100..24834
                     /gene="ypdB"
                     /locus_tag="ACUQR0_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HHV9108296.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24847..25704
                     /locus_tag="ACUQR0_001164"
     CDS             24847..25704
                     /locus_tag="ACUQR0_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9108297.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25707..28202)
                     /gene="ptsP"
                     /locus_tag="ACUQR0_001165"
     CDS             complement(25707..28202)
                     /gene="ptsP"
                     /locus_tag="ACUQR0_001165"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHV9108298.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28227..29264)
                     /gene="ypdE"
                     /locus_tag="ACUQR0_001166"
     CDS             complement(28227..29264)
                     /gene="ypdE"
                     /locus_tag="ACUQR0_001166"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HHV9108299.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29264..30347)
                     /gene="ypdF"
                     /locus_tag="ACUQR0_001167"
                     /pseudo
     CDS             complement(29264..30347)
                     /gene="ypdF"
                     /locus_tag="ACUQR0_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30362..31609)
                     /locus_tag="ACUQR0_001168"
     CDS             complement(30362..31609)
                     /locus_tag="ACUQR0_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HHV9108300.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31631..31957)
                     /locus_tag="ACUQR0_001169"
     CDS             complement(31631..31957)
                     /locus_tag="ACUQR0_001169"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HHV9108301.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32176..33141)
                     /gene="glk"
                     /locus_tag="ACUQR0_001170"
     CDS             complement(32176..33141)
                     /gene="glk"
                     /locus_tag="ACUQR0_001170"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HHV9108302.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33345..34601
                     /locus_tag="ACUQR0_001171"
     CDS             33345..34601
                     /locus_tag="ACUQR0_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HHV9108303.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34716..35042
                     /gene="ypeC"
                     /locus_tag="ACUQR0_001172"
     CDS             34716..35042
                     /gene="ypeC"
                     /locus_tag="ACUQR0_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HHV9108304.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35183..36421)
                     /locus_tag="ACUQR0_001173"
     CDS             complement(35183..36421)
                     /locus_tag="ACUQR0_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HHV9108305.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(36402..36458)
                     /locus_tag="ACUQR0_001174"
     CDS             complement(36402..36458)
                     /locus_tag="ACUQR0_001174"
                     /inference="COORDINATES: protein motif:HMM:NF049535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108306.1"
                     /translation="MKHSKGYVLEAQDDELSR"
     gene            36757..37959
                     /gene="nupC"
                     /locus_tag="ACUQR0_001175"
     CDS             36757..37959
                     /gene="nupC"
                     /locus_tag="ACUQR0_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HHV9108307.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38009..40121)
                     /locus_tag="ACUQR0_001176"
                     /pseudo
     CDS             complement(38009..40121)
                     /locus_tag="ACUQR0_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
     gene            complement(40329..40404)
                     /locus_tag="ACUQR0_001177"
     tRNA            complement(40329..40404)
                     /locus_tag="ACUQR0_001177"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40369..40371),aa:Ala,seq:ggc)
     gene            complement(40444..40519)
                     /locus_tag="ACUQR0_001178"
     tRNA            complement(40444..40519)
                     /locus_tag="ACUQR0_001178"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40484..40486),aa:Ala,seq:ggc)
     gene            40740..41099
                     /locus_tag="ACUQR0_001179"
     CDS             40740..41099
                     /locus_tag="ACUQR0_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HHV9108308.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41101..41493
                     /locus_tag="ACUQR0_001180"
     CDS             41101..41493
                     /locus_tag="ACUQR0_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHV9108309.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41545..42960)
                     /gene="gltX"
                     /locus_tag="ACUQR0_001181"
     CDS             complement(41545..42960)
                     /gene="gltX"
                     /locus_tag="ACUQR0_001181"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HHV9108310.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43219..43294
                     /locus_tag="ACUQR0_001182"
     tRNA            43219..43294
                     /locus_tag="ACUQR0_001182"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43252..43254,aa:Val,seq:tac)
     gene            43339..43414
                     /locus_tag="ACUQR0_001183"
     tRNA            43339..43414
                     /locus_tag="ACUQR0_001183"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43372..43374,aa:Val,seq:tac)
BASE COUNT        11564 a        10825 c        10093 g        10999 t
ORIGIN      
        1 gcattaatac tttggctgac atttttttcc cttaaattca tctgagttac gcatagtgat
       61 aaacctcttt ctcgcaaaat cgtcatggat ttactaaaac atgcatattc gatcacaaaa
      121 cgccatagtt aacgttaaca cttgcgatat tcatcgcatt tatgaaagta agggactata
      181 tttttataaa agttaacgtt aataattcac caaatttgct taaccaggat gattaaaatg
      241 acgcaatctc gattgcatgc ggcgcaaaac gcactagcaa aacttcatga gcaccggggt
      301 aacactttct atccccattt tcacctcgcg cctcctgccg gatggatgaa cgatccaaac
      361 ggcctgatct ggtttaacga tcgttatcac gcgttttatc aacatcaccc gatgagcgaa
      421 cactggggac caatgcactg gggacatgcc accagtgacg atatgatcca ctggcagcat
      481 gagcctattg cgctagcgcc aggagacgat aatgacaaag acgggtgttt ttcaggtagt
      541 gctgtcgatg acaatggtgt cctctcactt atctacaccg gacacgtctg gctcgatggt
      601 gcaggtaatg acgatgcaat tcgcgaagta caatgtctgg ctaccagtcg ggatggtatt
      661 catttcgaga aacagggtgt gatcctcact ccaccagaag gaatcatgca cttccgcgat
      721 cctaaagtgt ggcgtgaagc cgacacatgg tggatggtag tcggggcgaa agatccaggc
      781 aacacggggc agatcctgct ttatcgcggc agttcattgc gtgaatgggc cttcgatcgc
      841 gtactggccc acgctgatgc gggtgaaagc tatatgtggg aatgtccgga ctttttcagc
      901 cttggcgatc agcattatct gatgttttcc ccgcagggaa tgaatgccga gggatacagt
      961 taccgaaatc gctttcaaag tggcgtaata cccggaatgt ggtcgccagg acgacttttt
     1021 gcacaatccg ggcattttac tgaacttgat aacgggcatg acttttatgc accacaaagc
     1081 tttttagcga aggatggtcg gcgtattgtt atcggctgga tggatatgtg ggaatcgcca
     1141 atgccctcaa aacgtgaagg atgggcaggc tgcatgacgc tggcgcgcga gctatcagag
     1201 agcaatggca aacttctaca acgcccggtt cacgaagctg agtcgttacg ccagcagcat
     1261 caatctgtct ctccccgcag aatcagcaat aaatatgttt tgcaggaaaa cgcgcaagca
     1321 gttgagattc agttgcagtg ggcgctgaag aacagtgatg ccgaacatta cggattacag
     1381 ctcggcactg gaatgcggct gtatattgat aaccaatctg agcgacttat tttgtggcgg
     1441 tattacccac acgagaattt agacggctac cgtagtattc ccctcccgca gcgtgacacg
     1501 ctcgccctaa ggatatttat cgatacatca tccgtggaag tatttattaa cgacggggaa
     1561 gcggtgatga gtagtcgaat ctatccgcag ccagaagaac gggaactgtc gctttatgcc
     1621 tcccacggag tggctgtgct gcaacatgga gcactctggc tactgggtta acataatatc
     1681 aggtggaaca acggatcaac agcgggcaag ggatccgcgt cactcttccc ccttcacgac
     1741 cttcaataat atgcaatgca gcttcccgcc cgataatgtc atgtggaagc tgaattgtgg
     1801 tcagcggcgg taaaaacaga tgcccgacgc caaccagatt atcaaagccc attacagcga
     1861 catcctgcgg gatacgtacc cccttcgcca aaagaacctg ataagccaca aaggctgcgc
     1921 gatcgttacc acatatcaga acatcaaaat ctggtttgcc cgatttgaag tgagcattga
     1981 gtaaacttgc gagatcggtg tagtgatcat cacctgttgc catgtgaaat tgtttcacct
     2041 cagccagatc tcgtccagca tcacgccaag cctgctcaaa tccctgccga cgataccctg
     2101 ttgccaacgc actttccggt agccagaagc ataatggttg acgatagccc gccgcgagca
     2161 aatgctgtgt tgattcatat tgtgcagtgt aatcatcagg gatataactg ggtaacgctg
     2221 ggtcatccgc cacacagttc gccaatacaa tattttcacc atacagagac tcaggcagcg
     2281 tgatatgtcg cagccccatt gtagtataga taatgccatc cggacggtgg gcaagcagct
     2341 gacgtgccgc gcgggcagcg tcatcttcag aaaaaatatt gattaaaaaa ctattccagc
     2401 cgaactcgct ggcggtttgc tcaatggcaa gcagaatatc aacagagaaa ggagtggtag
     2461 ccgtgtcctg cgccagcacg gcgagagtcg acggcttacg tccttgagcg cgcatcttac
     2521 gggcggaaag atcaggaaca taattcaggg tctggattgc ctgcaatacg cggtcacgcg
     2581 ttgcaggacg cacagattct gcattatgca tcacccggga gactgtcatc atcgacactc
     2641 ccgccaggcg tgcgacatcc tttaatgaag ccatacccaa gccgtttgcc gtaaaacggg
     2701 cactgtagca gaaacagacg tcactggcga gatccaacgc cctatcacct gacacagcaa
     2761 tacaataaaa aataacaata attcccggac aattgtcccc aattccgcct ctgttctcgc
     2821 caacgagtaa ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag
     2881 aagaaaatat aaatatggat cattgtgctg ccactgtatc ccgacatcag cccggaaatt
     2941 attgcgattg ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc
     3001 tggctggtga agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca
     3061 gcgacattta tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc
     3121 taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc
     3181 cgttggtaaa ggatctggtt gctcttaaag aaaccacctg gtttaatcct agcacgacct
     3241 cattggctga aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg
     3301 cgcgcttatc ccgttttgca ccctatctgg caaaagcatt tgctgaaaca gctgccactg
     3361 gggggattat tgaatcagaa ctggttgcca ttccggctat gcaaaaacgg ctggaaaagg
     3421 aatatcaaca accgatcagc gggcaactcc tactgaaaaa agatagccat ttgcccattt
     3481 ccggctctat aaaagcacgc ggcgggattt atgaagtcct ggcacatgca gaaaaactgg
     3541 ctctggaagc ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt
     3601 ttaaacagtt ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat
     3661 caatcggcat tatgagcgcc cgcattggct ttaaggtgac agttcatatg tctgctgatg
     3721 cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc
     3781 aagattatgg tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt
     3841 tctttattga tgacgaaaat tcccgcacgt tgttccttgg gtattccgtt gctggccagc
     3901 gtcttaaagc gcaatttgcc cagcaaggcc gtatcgtcga tgctgataac cctctgtttg
     3961 tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcggg cttaaactgg
     4021 cgtttggcga tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt
     4081 taggcgtcca tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc
     4141 ttaccgcagc ggatggcctt gcagttggtc gcgcatcatg ctttgtcggg cgggcaatgg
     4201 agcgtctgct ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct
     4261 ggctggcgca ggaagaaggt attcgtcttg aaccttcagc actggcgggt atggccggac
     4321 ctcagcgcgt gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaat
     4381 tgcgtaatgc cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga
     4441 tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa cgcaacatcg caatcctttc
     4501 cctgggtgag cgatgctgcc gatggcgcag acttaagatc cccggtctta cccgctataa
     4561 ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
     4621 ccaaaaccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
     4681 tagaaagtga ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac
     4741 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg
     4801 ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg
     4861 acgtaccaac tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa
     4921 atttattatc tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa
     4981 cggcgaatcc ctggaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
     5041 atgtcacaga acgccagtaa tagcaaaccg catacatcaa aaaactgaat gtcactaaca
     5101 cccgcatgtc tattttattg ccataacgtc ctatcaaagg tgaaatcaat agtggcatga
     5161 tgccgatggg cgcataagca agcccggccc atatcgcatt ataccccatc gttttctgga
     5221 gtaactgcgg cataaggacg atcgctccag agtaaaataa atacgcgcag gtgatactca
     5281 caataccaat ggtgaaatta cgggacttaa acaaactgag atcaagaatc gggttctctg
     5341 aggtcgattc ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta
     5401 ttattgtact cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc
     5461 caccaacacc gagcactaac agggtcagtc ttggtagatt catttttacc ggtgaagttt
     5521 cagtttctct tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga
     5581 cattgattaa aaatatccaa ccccagctaa agttatcgca aatatagccg cccaatatcg
     5641 gcccacatat cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt
     5701 tttctggtgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
     5761 ttaatccctg aacgactcta aaaaatatca gcacatcaag attggtcgat aaactacaca
     5821 ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa taaccttaat tcgcctattc
     5881 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga
     5941 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
     6001 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtaa
     6061 cgcaccataa cgtcccaccg gttaatggtg ccggagttga tttagtgatt gccattaaag
     6121 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
     6181 taaagcctta ctggtataag caggcatgga ggtcacggtt gaagccagat caggcatctc
     6241 agcaatgtct tcgtccttcg tatcaatagt tgcagtcatc gataaaccaa tgcgcaaggg
     6301 gtgttccatg agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt
     6361 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
     6421 attgatccct gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat
     6481 attgactgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
     6541 ccacatttga cgtgccggta cgacagccat taacgattgt ccggaactca ctgtttcgcc
     6601 gacctgaaca cttcgctggg caatatagcc agtaaccgga ctccttatat ccgtacgttt
     6661 aagcaccaac caggcttctt tggttgcatc cgccgcttca actacttgtg gctgacggtt
     6721 taatggtgtg ttcattacta aagctttatt cgctttataa gcctggatag cggcattcag
     6781 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac
     6841 cccctgcttc gctaaaggca cacgacggtt ataatcttct aaagattgtt gatactgaat
     6901 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg
     6961 ccgaacaata tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt
     7021 atccagtgaa actaaaatgt caccctgtcg aacgtagttc gtatctttat gattaactac
     7081 agtgacacta cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc
     7141 atctgtacta atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc
     7201 aataaataaa actatcacca ataaagaaaa gtattttttt ctgttagact gttttttatt
     7261 tgaattaatc tgttccacta ttatctctca tttcgcatag atgaaattta tgaattgaat
     7321 gaaaagagta ttcattcttc atatcaaaca cagtgcatgt gttgcacatt atcccaggaa
     7381 cgttggaggc ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa accgtggctt
     7441 ttgcaataca attcttacgc ctgtaggatt agtgagaaga cttatagtgc taacttgaaa
     7501 ctataaatca tcggtataat ccctgatttt attgttgaca tttcatttat gccgactatt
     7561 tatatggtat acttgtcgaa ttatcttaaa ggaaactcag attttcttat ttttattgag
     7621 aaaatgagat gacgccttat gtctgtatta ctacagggag aagggagatg cttcattgca
     7681 aagggaataa tctatgaacg caataattat tgatgaccat cctcttgcta tcgcagcaat
     7741 tcgtaattta ttgatcaaaa acgatattga aatcttagcg gagttaactg aaggcggaag
     7801 cgccgttcag cgggtggaaa cacttaagcc tgatatagtc atcattgatg tcgatatccc
     7861 cggagttaac ggtatccaag tgttagaaac gctgaggaag cgccaatata gcggaattat
     7921 tattatcgtc tccgctaaaa atgaccattt ttacgggaaa cattgtgctg atgctggcgc
     7981 taatggtttc gtgagtaaaa aagaaggcat gaacaatatc attgcggcta ttgaagctgc
     8041 aaaaaatggc tactgctatt tccccttctc tctcaaccgg tttgttggaa gtttaacgtc
     8101 cgaccagcaa aaactcgact ccttatcgaa acaagaaatt agtgtcatgc ggtatattct
     8161 tgatggcaag gataataatg acattgcgga aaaaatgttc atcagcaaca aaactgtcag
     8221 cacgtataaa agtcgcctga tggaaaaatt agaatgtaaa tcactgatgg atctttacac
     8281 attcgcgcaa cgtaacaaaa tcggctaatc acatgaagtt tttaccctat ttttttcttc
     8341 tctgttgtgg tctttggtcg accataagtt tcgcagacga agattacatc gaatatcgtg
     8401 gcatcagtag taacaaccgt gtcacacttg atccagtacg tctgagcaac aaggaattac
     8461 gttggttagc gagcaaaaaa aatcttgtga ttgcagtaca caagtcccaa acggctacct
     8521 tgttgcatac cgattcgcag caacaggttc gtggtattaa tgctgattat ttaaatcttt
     8581 taaaaagagc gttaaatatc aaattaacac tccgggaata cgcagatcat caaaaagcaa
     8641 tggacgcgct tgcagaaggt gaagtcgata tagtgttatc acatttagtt acttcgccgc
     8701 ctcttaataa tgacattgct gcaaccaaac cattgataat tacctttccg gcgctggtaa
     8761 ccacccttca cgactcaatg cgaccgctta cctcaccaaa accagtaaat attgctcggg
     8821 tagcaaatta ccccccagac gaggtaattc atcaatcatt tccaaaagca acaattatct
     8881 cttttacaaa tttatatcag gcattagcat ccgtctcagc tgggcacaat gattacttta
     8941 ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg
     9001 tagtgaaata ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatctg
     9061 tcattcttaa tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg
     9121 aagtatcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac
     9181 tcactgaaca tgaaaaacag tggattaagc agcatcccaa tttaaaggtg ctggaaaatc
     9241 cttactcgcc cccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg
     9301 acattcttaa tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac
     9361 acaatatcca tgctggaaca caacttagcc ccggaggatg ggatataata cctggcgcta
     9421 tttatagtga agatcgagaa aataatgttt tatttgctga agccttcata acaacgcctt
     9481 acgtttttgt catgcaaaaa gcgcctgaca gtgaacaaac attaaaaaaa ggaatgaaag
     9541 ttgccattcc atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg
     9601 aatggataca ggtcgataat gccagcgctg catttcacaa ggttaaggaa ggtgaacttg
     9661 atgctctggt cgcgacacag ctaaattcgc gttacatgat cgatcattac tatcctaatg
     9721 aactttatca ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc
     9781 gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg
     9841 aagttctgcg cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg
     9901 acctatatag cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta
     9961 gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg
    10021 atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt
    10081 atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca
    10141 ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgattca ccctttaaag
    10201 atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca
    10261 cacaggtatt tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat
    10321 tatgcaatct tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg
    10381 aaacgcgtga tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg
    10441 tagcaaaaag tcagtttctg gcaacgatga gtcacgaaat aagaacacca ataagctcta
    10501 ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg
    10561 cgatttcact tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg
    10621 atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta
    10681 ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat
    10741 taagttgcag cagtacattt cctgatcatt acctggttaa gatcgaccct caggcgttta
    10801 agcaggtctt atcaaattta ctgagtaatg ctctcaaatt taccaccgag ggggcagtaa
    10861 aaattacgac ctccctgggt cacattgatg acaaccacgc tgttatcaaa atgacgatta
    10921 tggattctgg aagtggatta tcgcaggaag aacaacaaca actgtttaag cgctacagcc
    10981 aaacaagtgc aggtcgtcag caaacaggtt ctggtttagg cttaatgatc tgcaaagaat
    11041 taattaaaaa tatgcagggc gatttatcat tagaaagtca tccaggcata ggaacaacat
    11101 ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag
    11161 aacaacccat cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca
    11221 acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg
    11281 atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg
    11341 ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt
    11401 ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa
    11461 gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt
    11521 taagtcagtt acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgaag
    11581 ccctgaaaaa taatacggcg aatgatctac aactgatgca ggagattctc atgactttcc
    11641 agcatgaaac gcataaagat ctacccgctg cgtttcatgc actagaagct ggcgataaca
    11701 gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa
    11761 agttgattaa tattagccat cagttagaaa taacacctgc ttcagatgac agtaagcctg
    11821 aaattcttca gctgctgaac tctgttaaag aacacattgc agagctggac caggagattg
    11881 ctgttttctg tcagaaaaat gactaaattg cggctcccac aatgttctaa tgtgggagct
    11941 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
    12001 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
    12061 attacccggc atcattattc ctccggcttc aatcaacata tttctcgcct gagtttgtgg
    12121 caaattaatg gcctcagcca cacttaataa cggcgcgacg ggcacaccaa cttcatgtat
    12181 tttggctaac caaacttcag ctgcctgcgt ttttaacgtt cgctcaatat attgtttaag
    12241 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
    12301 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
    12361 aatcggctta tcctgagtat cgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
    12421 caggcgttgt ggtgacttcc ctgtcgcaat atatgccatc agaccatgct ccagaaaact
    12481 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
    12541 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
    12601 ggtaccaaca cgcactggcg gagcatcagg gtaccctgtt tccatcatta tcccactcat
    12661 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
    12721 tgacgatgaa gcatatatga ggcgcgggtt gatttcttgt aacctttccc atgaaaaccc
    12781 cagtttttcc attgtaccta ggcgaaaatt ctcagctaat acatcagctt gtttgagcat
    12841 atttataaat atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc
    12901 atgattaata aaactgtaat agagtgactg tccatccaca tagggaccaa atgtgcgggt
    12961 atcatcacca tgacctggtg gctcaacttt aattaccctt gcgcccatat tataaagaag
    13021 ttgagttccg aaaggtccat taaggacatg tgtcatatcg ataactaata agccttcaaa
    13081 cggccctttg ctttcattat ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa
    13141 agacacttcg gcatttatgt aaaatcaata acatgattaa ttatgaaacc agtcgactga
    13201 cataaatcca caagggagcc gtgacgacaa agcccagtac gctcacagcc aatgacgctg
    13261 taccagtacg ggtgtagaca ttaaaccggc tggcaataat gattccggag aatgccggcg
    13321 gtaatgcgcc tgccagtacc atcatctgca aatgttcgct gttcaaatga catgccatac
    13381 caacgagaag cagcgccagt ggcatcagaa tcaacttcag gaaggtgtta taagcaattt
    13441 cggcactgaa ctcgaattta tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc
    13501 ctgagttagc cttcgcaatc agattaaagg ttgggtccca tgctgccgga atttttaccc
    13561 caaccagcac caggatcgtt gccagaacag gtgcccatac tactggctcc tttgccgcag
    13621 aaattaatgc gctcagatta ctattcttct taccatccgc tcctgaggaa ggattcagca
    13681 aatacagacc aataggaata gtaattgcgt taacaataat agaaataatt gctaccacta
    13741 aacctgttga tacggaatca ccataaatag gatcgagaac tgcaaacccc aggaatccaa
    13801 tggtaggtga acccgcaatt aatgcacata cagctgcttc tgcatgggta cgtttaaaaa
    13861 atttgtagca accaaaccag gagaaaaaga aacatccgac aataaccaca agtgatacaa
    13921 gggtcagacg agtgtccgca aaaatcattt ccctgtttgc ccgggtaata gaaacaaata
    13981 gagccgcagg aagagcgtag tttaatacca gtttattaaa tgcgcgggct tgatcttctg
    14041 aaaaagtttc tcgtctgccg ctaaaataac ccaacagcat gattacgata ataggcaata
    14101 aatcgccaat aaaaaatgtt agcataatag tatttccgat aagggtcccc tcaccatcgc
    14161 ataatgagtt ggcaagagga gacgatgtca gtatttaatt accagcgact tgttttgggt
    14221 taagtttggt aatatggcca ctttcagttc ctgctgcagg gtcgataacc acattaataa
    14281 tggtcggttt gcgcgactga ataccggtgg ttaaagcatg acgaagttca tctgtcgtgg
    14341 tgacgttata gccaacgcca cgaaacgcat ccattaattt gtcatacctt gcatggtgca
    14401 acagatccgt tggtgatggt gcaccagcgc cactgagatc aacaccgtct cctctgtaga
    14461 tgccgccatt attaaaaata acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa
    14521 tttccatccc actgaaacca aaagcactat caccttcaat ggcgacaacc ggagaaccag
    14581 aagtcacgct agcaccgatg gcatagccca taccgatgcc catgacaccc caggtaccac
    14641 aatccagacg acgacgtggt ttatacatat caataatatt tcgtgcatta tccagggtat
    14701 ttgcaccttc attaactaaa taaatatcct ggttctcgcg caatacgtcg cgcacagcac
    14761 ttaatgcatt aaagtaattt aatggttgag tatctgtact taatttttca tgcatttttt
    14821 gtgcattttg ctgcttgtgg atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg
    14881 tgttttgttt cagttctgcc agcatacctt gcatactgga tgcaatatca ccaacgactg
    14941 gcacagcaat ggggcggttg ctgtcaattt cctgcggttc aatatccagt tgaataaact
    15001 gtgtatctgc cgcccatcct tttttaccgt gtgccaataa ccaattcagt cgtgcaccaa
    15061 caagcatgac aacgtcagca tttgccaggg caaacgaacg cgcagctgcc gcagaaagtg
    15121 gatgcgtatc ttcaaggatc cctttcgcca tagacattgg caggaatgga atctgagcac
    15181 tttcaataaa ttcacgaagc tgttcatcag cttgtgaata cgccgcgcct ttgccaagga
    15241 taattaatgg ccgttcagct tttgctaaaa gcgaaattgc gctagtgact gacttcgggc
    15301 atggcaataa tgctggcgac ggattttcaa ctttaacaat cgtggttaac gcttcgtctt
    15361 tttccatcgt cgcggccagg acatttgctg gcaaatcaag ataaactccg ccagggcgac
    15421 ccgatacaga gacccggata gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa
    15481 cgcgaaatgc tgctttggca tacggttttg ccgcattcat ttggtccagc tcttcataat
    15541 caccttgctg taggtcgacg atcgcgcggt cgctggagcc gctaatcatg ataatcggaa
    15601 aaccatttac cgttgcgttg gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa
    15661 ctgtcaggca gatccccggt ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg
    15721 ccgactgctc atgacgaaaa ccaatataac gaatgccttc cgcctgggca tggcgtgcca
    15781 tatccgtcac aggaatacct acaacaccat aaatagtgtc aatattattc tgttttaatg
    15841 cttcaacgat gatatgcata ccatctgtca tttgaagttg atctgacatg attaataacc
    15901 tcgaaatttt tataaattgg gagccagaag cccccgttaa tatcagatgg cgtggttttg
    15961 cttcattgca gcaatttcat cgtcgctata acccagctcc tgcaatacag cagcggtatg
    16021 ttcacctaat agcggcgcag ctttaatatc cggcgtaaag gcagagaatt tcattggaca
    16081 accaacggtc agatattttc cacgcaacgg ttgttccact tcaacaacac tgccactttg
    16141 gcgcaaagag ggatcaagtg aaatttcttt catacttaaa accggtgcac aaggaatatc
    16201 aaactgagtc aaataggcca ccgcttcatg tttatcaata gtgacagtgt atttttcgat
    16261 ttcagcaaaa atatcgaaaa tatgtggctg tcgggcatgg gctgtactgt atgccggatc
    16321 ggtaatccat tctggtttgc cgatggcttt acaggtgttt tcccagtttt gctcctgaat
    16381 agtgaaataa atataggcgt taggatcggt ttcccagcct ttacatttca ggatccagcc
    16441 aggctgaccg ccaccacctg cattaccacc gcggggaact gcatcaccaa atgtaccatt
    16501 cggatactgc gggtattctt ccagataacc caatttatcg agacgctgct ggtcacgtaa
    16561 tttcacgcgg caaaggttca atacggcatc ctgcattgac atggtgactc gttgcccacg
    16621 ccccgttttt tcgcgatgca gcaaagcagc aagtaaaccg atcagcaaat gcattccggt
    16681 gttgctgtca cccaacgctg cagcgcttac cagcggcgga ccatcccaaa aacccgtagt
    16741 ggatgccgcg ccacccgctg cctgagcaac gttttcatag gcttttacat tcacataagg
    16801 cgaacactca tcaaaccctt tgatcgaacc aaaaatcaga cgtggattga tttcttgaat
    16861 atgctcccag gtgaagccca tgtgatcaat ggcccctgga tgaaagttct cgactaagat
    16921 atcagcttcg cggatcagct tttccattac ctctttgcct tccgctgttt tggtatttaa
    16981 ctcaatagaa cgtttgttac tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat
    17041 atctcgcagt tggtgacgcg ttacgtcacc aacgccggga cgttcaattt taataacgtc
    17101 agcgccaaac caggccagca tttgagtaca agatgggcca gattgcacac cggtgaaatc
    17161 gagaacttta attccttgaa gtggagttga catagcaata cctattagtt aattaaatat
    17221 gtgttaattt agcgtttatt taaggcataa cgccctctat aaataatctg caaaatatct
    17281 tcgtagcggc actcgatatt tttaaattcc tgtatcaagt actgaaaatt attttatcgc
    17341 cacttccaat tcctttctgc atggtaagta gtgaggacga aaagaagaat atgataaata
    17401 ttgattcgtg gtaatcagag agagtcgcga catttgtgat gttattctta caactgtaag
    17461 aagcgccggt gatttacaag taagagtatg attacgcttt ttaaagcgta ccataaagca
    17521 taacataata aatgatttgt gatgacacgg gattattgcg gtttactttg aggaaaataa
    17581 aatgcgtgtt tatagaaccg cgagcgccaa ataattttat gaccacaacc tgtcgtattg
    17641 tggtcataaa aaattgtcgg ctggagggag ttaaatcctt tacctcaata tactgcctgt
    17701 cagcattagt tcacaaacag tgcttcgcta tcgacgatga ttccatcttc cgcaccttta
    17761 agcgccaggt ttgcctcgta gtattgcttt ttatcaagca gtttctgcgc atcagcaagt
    17821 gcattacgag tctgtttaag cggcatcaga tattggtttt ccattacgcc aacaccggcc
    17881 aggcgaagct cttccattgc gcccttttta tcccctttcg ccattttttc attggcaatt
    17941 tttatcgcgg cttctttttc gggtgttgct acataactct cagaaattcc gacagatgca
    18001 ttaatgacaa tatattggtc atcattgaga ttggtctttt tacccggctt agcgaacttt
    18061 gcccattcgg tactgtcatc tgacagcaaa gcggaagctt cattggtcag ttcttttgcc
    18121 ttttccggat cgccatgaaa cagagccagg cgcgctacct gcacgtcacg catcgcatat
    18181 aaaccttgtt cagagatacg ctctgctgct gccgttttct gggtttgctg agtttgctgt
    18241 tgtgccgcta ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg
    18301 accatcgtag ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa
    18361 cgccgccatc atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa
    18421 agggttatct tacaattgta gttattatcg caaagcggca tttagtctta cagttgtagg
    18481 aatatgcctc ctgtcgcaga gaactattct tatataccac aaagagatgt catttattat
    18541 attgatgtaa atattgatta attatatgta aatacagcca taacatcgta tggttctcag
    18601 tgaaccattt tcaggataac tttctatgaa agtaaactta atacttttca gcttattttt
    18661 attggtctct attatggcat gcaatgtttt tgcattttcc atttctggtg gtgtaagtga
    18721 gaggagctat aaagagactg aaaaaacatc agcgatgacg accacacact ctacaaaact
    18781 tcagccatca caggcgattt tgtttaagat gagagaagat gtgccaccat taaacctcac
    18841 agaagaaatc acgcctactt atccgacaaa ggcgaattat cttattcatc ctgtgcgata
    18901 gcgtctgtca catggcaggt gaacaatcat gtctgatgct gatgtgaaca cctgcccaaa
    18961 tgagccatcc tgacccacta tcgcctgcca taccccacca taaagcgaat catcaagcca
    19021 caagccgggg caagaaacat aaatatcccc gtcaccagtt cattaccgcc agttaaccac
    19081 atggtgaagt aataaatgag tgcagcgagg atcaccatgc cgaagaaaga agagaccgcc
    19141 gataccacgt tcagtacctg accgatgtag ccgcttacgc agacaataca cagggcgaga
    19201 aacatccata aataaatcat agctcaacct cctgccgcgc taaaaaacct gtaacttcag
    19261 tcactttaat agtaagacaa tccttaacct ctggcaattt ttgctaagca tcaatgaaaa
    19321 cagcacgtta aatgcaacgc tattgcgtgc ggaaaatttt ttactttgca cgattaataa
    19381 tcagtagctg aaagcagtca gccagtgata aagtagagcc accgcattca tgctcctcgt
    19441 ggttatgtcc tgactagtct ttacactctt tacaggaacc attgtcgtac atgatggccc
    19501 aaccaattga gtgtaccgct gtcgacatgg aatcttctgc gatacaacaa ttcgtatcta
    19561 cagaaggtaa ctatgtttcc acaatgcaaa ttttcccgcg agtttctaca tcctcgctac
    19621 tggctcacat ggtttgggct tggtgtactc tggctttggg tacagcttcc ttatcctgtt
    19681 ctctgctttc tcggcacgcg tattggcgca atggcgcgac cattcctgaa acgtcgtgaa
    19741 tctatcgccc gtaaaaacct ggaactttgt ttcccgcagc attctgcgga agaacgcgag
    19801 aagatgattg ccgaaaactt tcgttcactc ggcatggcgc tggtagaaac cggcatggca
    19861 tggttctggc ccgacagtcg cgtacgtaaa tggtttgatg ttgaagggtt ggataacctt
    19921 aaacgcgcac aaatgcaaaa tcgcggcgta atggttgtcg gcgtccattt tatgtcgctg
    19981 gaactgggcg gccgcgtgat gggactgtgc caaccaatga tggctaccta tcgtccacat
    20041 aataatcagc tgatggaatg ggtgcagacc cgtgggcgca tgcgctctaa caaagcgatg
    20101 atcggcagaa ataatctgcg cggcattgtc ggtgcactga agaaaggtga agcggtatgg
    20161 tttgctcccg atcaggatta tggtcgtaaa ggcagctcct tcgcgccgtt ctttgcggtg
    20221 gaaaatgtcg ccacaaccaa tggcacctat gttctctccc gtctctctgg cgcagccatg
    20281 ttgaccgtaa cgatggtaag aaaagcggat tacagcggat atcgtttgtt catcacccca
    20341 gagatggaag gctacccgac agatgaaaat caagccgctg cttatatgaa caagattatc
    20401 gagaaagaga tcatgcgcgc accggagcag tacctctgga tccaccgtcg ctttaaaacg
    20461 cgcccggtgg gagaatcgtc gttgtacatt taacaaactg ctcttactct ttccctcctc
    20521 aaatcggcca tgataacctc atgattttta tagctttttt cttaagtttt ttccttcgct
    20581 gaatatctaa ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt
    20641 actaaaagta aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc
    20701 cgtctcgagc agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt
    20761 taaggtcgct ttgcccggtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt
    20821 aacagtaatt acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg
    20881 gtcatcgttt gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa
    20941 acaggagcca tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt
    21001 gaatatgttt gctgctgacg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc
    21061 tgatggcctg acgaatacgg tcgtggtttt caatcagtgc aaagcgaaca tgcgtatcac
    21121 catagtcgcc aaagccaatc cccggcgaga cacaaacctt cgcttcgtta aggagcttct
    21181 tggcaaattc cagcgatccc atggccgcat atggttccgg gattttcgcc cagacataca
    21241 tcgaagcctt cggcatttcg accatccagc ccgcttcatg cagcccttta accaatacat
    21301 cacgacggcg tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctcgagtg
    21361 ccgcaatcgc cgccacctgc aacggcgtaa aagtgccgta atcgtgatag cttttgattc
    21421 gcgccagggc actgaccagc gttttgttgc cgaccataaa gccgatacgc cagcccgcca
    21481 tgttgtagct tttcgacagc gtaaagaact cgaccgccac atcgcgtgca cccggcacct
    21541 gcataattga cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcatgga
    21601 ccaccagcac atcgtagcat ttcgccagcg ccacaacctt ttcgaagaac tccagttcca
    21661 cgcattgcgc ggttgggttc gacgggaagc cgaggatcat catcttcggt ttcggataac
    21721 tttcacgaat ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg
    21781 agcgtacctg cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg
    21841 gcaccagcac cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt
    21901 tcgaaccaat agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct
    21961 gataccagcg ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat
    22021 gcgtgtccgg gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac
    22081 cgtccgggtt acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt
    22141 tcagttcagc ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac
    22201 gttcagggcg agtgtcagcc ataattttct cagattaacg ttagcgcccg gaccgtccga
    22261 gcgacgctgc cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag
    22321 cgttttagaa catttttatc aatggattta aatgaggaac tccacttcaa aaacaggaaa
    22381 tttttctacg ctaacaagaa aatcaccacc aattttatta acattataat ttcaataaat
    22441 ttaccagcta gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat
    22501 ccccttttaa atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt
    22561 acctatcata gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc
    22621 tgctggcggt cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta
    22681 tccgcctgtt tcgcgaactg ttgcacaagt cggcgcactc cccaaaagaa ttgctcgccg
    22741 ttaccgccat tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag
    22801 gctcgctggt gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt
    22861 gggtaggcat cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg
    22921 gcgtgacggc gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga
    22981 taaacctgaa aatccccaaa gcacagcgct ggcgctggat atcgccaaca aaacgctacc
    23041 gctgtttcgc catgtcaata gcgagtcatt acgcaaggtc tgcgaaatta tccgcgatga
    23101 cattcacgcc gatgcggtgg cgattactaa taccgatcat gtgctggcct atgttggcgt
    23161 gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc gtcaggcgat
    23221 gaattacgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac cagagattca
    23281 ttccatgctg gtgatcccat tgtgggagaa aggggtcgtg accggaacgc tgaaaattta
    23341 ctactgccac gcgcatcaga tcacctcgtc attacaggaa atggcggtcg gtctgtcgca
    23401 aatcatctcc acgcaactgg aggtttcacg cgccgagcag ctacgtgaaa tggcaaataa
    23461 ggcagagctt cgcgccctgc aaagcaaaat taatccccat tttctgttta acgctctgaa
    23521 cgctatttca tcgtcaatcc atctgaatcc ggataccgct cgccagttga tctttaatct
    23581 gtcgcgttat ctgcgctata acattgaatt aaaagacgat gagcaaatcg atatcaaaaa
    23641 agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg gcgacaagct
    23701 gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc tgctgatcca
    23761 gccgttggtg gagaacgcca ttgtccacgg tattcagcct tgcaaaggta aaggcgttgt
    23821 caccatcagc gttgcagagt gcggaaatcg ggtacgcatt gcggtgcgag ataccgggca
    23881 cggcatcgtt ccaaaggtga ttgagcgggt cgaagcgaat gaaatgccgg gcaataaaat
    23941 tggcctgctg aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc tgcatatccg
    24001 ccgcctggag ccggggacgg aaattgcgtt ttacattcct aaccaacgca ccccagtcgc
    24061 ctcacaggct acgttattgc tttgagccgg agtgatattg tgaaagtcat cattgttgaa
    24121 gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag ccagatggag
    24181 attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca taaccgcgtc
    24241 gacgccattt ttctggatat caatattccg tcgctggatg gcgtgttgct ggcgcaaaac
    24301 atcagccagt tcgcccataa accgtttatt gtgttcatca ccgcgtggaa agaacatgcg
    24361 gtagaagcgt ttgaactgga ggcgtttgac tacattctca aaccgtatca ggagtcacgt
    24421 attaccggga tgctgcaaaa actggaagcg gcctggcaac aacagcagac cagcagtacg
    24481 cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga tgagcgaata
    24541 atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat gacctttgtc
    24601 tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgaattttg cagcaaactg
    24661 ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa caaaatacgc
    24721 gaaatcgaac cgtggtttaa taacacctac attctgcgac tgaaagatct ggattttgaa
    24781 gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt taatgcatct ttaatcggga
    24841 actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga tctgttcagc
    24901 ggcctagtgc ttaacccgca actactgggg cgcgtctggt ttgccagcca gcctgcctcg
    24961 ttaccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct gcgcggcgaa
    25021 tacggcaacc tgctggaagc aaagcagcag cgaatggtgg aaggagaaat gctgtttatt
    25081 ccggcacgcg cggctaattt accgatcaac aacaaaccgg tgatgctgtt aagcctggtg
    25141 ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc gttgttgcat
    25201 cctgctcgcc agacccagct tcccagcctg caacgcggtg aaggtgaagc gatgcttacc
    25261 gccctcaccc atcttagccg ttcgccgctg gagcagaata tcattcagcc actggtgtta
    25321 agtttgctgc atctttgccg caacgtggtg aatatgccgc cgggtaattc acaaccgcgc
    25381 ggcgattttc tctatcacag catttgtaac tgggttcagg ataattatgc ccagccgctc
    25441 acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc aaaactgttt
    25501 gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt gggtgcgaat ggcgaaggcg
    25561 aggatgattt tgcagaaata tcatctgtca attcatgaag tggcacagcg ttgcggtttt
    25621 ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttagtctgac gccgggagag
    25681 tacagcgccc gttttcaggg ctaacgtcag aaggttaatt ctgtttccag cagcgtcagg
    25741 atactttgtg catcttgcgc ggcaaacaac gactggcgga agtttttatt caccagttta
    25801 cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcacc cagcgtcagc
    25861 atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt cgccagccgg
    25921 gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat cgctacgcca
    25981 aaaccaacgc cggtggtaac aatctcttcc cgctgccaga catcttcttc cagctcaaac
    26041 ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca ggaactggat cgcctgctct
    26101 ttattgctaa aatcctgatc aacaaagata ttctccagcg ccagcagtgg gcgaacgtct
    26161 tcttccggcg taaaggcggt gagtaacgct tcaatttcct gcgcactgcg gcattcacat
    26221 gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg gcttttcacc
    26281 gccggaatac gcgggctact catactcagc tcatccaggc ccagcccaag cagtagcggc
    26341 agataacggc tttcaccgcc cagttcaccg caaatgccta cccatttgcc ccgctgatgc
    26401 gcagtggtaa ctatttgctg caacatgcgc aggaacgatg gcgtaatcgg gttatatagc
    26461 ggcgatacgc gcgggttatt acgatcgacc gcatacagat actgggtcat atcgttggag
    26521 ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca caccgacggc
    26581 acttccacca tgatcccaag cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc
    26641 tcaacgatcg ctttttggat ctcgcctttc acccataaga tctgatcgag gctgtgaacc
    26701 atcgggatca tcaactgggc gttgccgaaa ctggcggcgc gcaaaatggc ccgcagttga
    26761 gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg
    26821 ttctcttcct ggggaatatt cagataagga atgcttttat cgccgccgat gtccatcgtg
    26881 cggaagataa tcggcttgtc gcccgccgcc agtagcacct gctggtaggc ttcaaattgc
    26941 tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg gaacagcccg
    27001 acaccttccg cgccgttggc aaacgcgcct ggcgcttcca gagcggtgcc gatattcgcc
    27061 gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcagc ctgcgcctgt
    27121 tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct caccgcgtca
    27181 ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg cccggcataa
    27241 cgggcaatcg catccagcgg caagccactc agtaccggga tcgccgaggc acgggccaga
    27301 atcagtgtat gcgaggtgcg cccggttttc tccagaatca tacccgcaag atttttcaaa
    27361 tcaaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt cggtttttcc
    27421 agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa ctgttcgctg
    27481 atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc agatagtttg
    27541 gcgcaaacct gctccatatt gctgatgatc gccgccccca gtccctgatg ctgttctgtc
    27601 atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgagcgctg
    27661 aggatagttt tgctttcgcc gtcacgctca cgcaattgct gattcagttg ctcggcaagc
    27721 gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat tgcccgataa
    27781 ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc gcttgccagc
    27841 acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag cggatgcgcc
    27901 gtcagttctg cctgcgtcgg ctgaacgctg tcgctgtcga taaagcgcac caggatgtac
    27961 tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag gctgcaactg
    28021 tcattaaata aggtgccagt tccaatcaac gccagcgagc ttttagcatc agcctttgcg
    28081 ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg ttctttaagt
    28141 tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac
    28201 ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca
    28261 cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcgcggcaa
    28321 tcggcaatcg atgccgcgca atgtccgtgg cgggtggcag gccctattac caatgtgggt
    28381 acgccagtac cggttaagtg caccgctcca ccgtccgtgc cgccgttact gaacatatcc
    28441 gcctgcaacg gaacgccaat ttctgccgcc acggtttcaa tccacgcggt aagttttggc
    28501 ggcgcaatca gcgatttatc gcttaacacc agcatcggcc cgttaccaat ctgacgatgg
    28561 ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac aatggcgacg
    28621 tccggcgaca ccgcgcgggt agcagtttgc ccgccgcgta atcccacctc ttcgctggaa
    28681 cttgccacca gccacacttc cgcaggcagt tcggcgtcgt gcagctcacg tagtaacgtc
    28741 accagcagat agcaactgag gcgatcatca aaggctttgc ccatcacccg ctggtgaggg
    28801 aggacctgaa aagtggtatc gaaggtgacg cgatcgcctg gacgaattcc cgcctgcatc
    28861 acttcgtcat aggtgcgcgc gccaatgtcc acgcgcatgg cgctaacgtc attcccctgc
    28921 cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtggtgat gcgcaccggc
    28981 tgcagctggc gggcagccat gcgcacgttg ccaaccggca gcacgtcaat agcgccttcg
    29041 cgggagatgc tgcgcaccat aaatcccact tcgtccatat gggcgcagat catcaccttc
    29101 ggacctgtcg attcattgag gcggatcagc accgatccca gaccatcaaa tcgcacttct
    29161 ttttgcaggc gagccgcttc ttccagcagg atctgccgca cttcctgttc cgaggaggcg
    29221 atcgcatctg cctcgctcaa cgcttttaat agcgataaat ccattatgcc tctcccgtga
    29281 gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcggggta accagcacaa
    29341 catcttcgat gcgcacgccc ccttgcccta gcaaataaat ccccggctcc acggtcagca
    29401 acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggatct tcatggactt
    29461 caatgccgat agcgtgacca gtgttatgac cgaaatagtc gccaaatcca gcctcggtaa
    29521 tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac gccggggcga attgcagaga
    29581 ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc aaacagcggg tgagattcgg
    29641 cgctcacccc ttcgccattc accagcaagg tgcgcgtcat atcagcagta gccctgatac
    29701 agcgcaccaa aatcgagagt gacaaactcg cccgctgcaa caatcttgtc gctggctttg
    29761 ccgtgcggca gcgccccgcg ccagccgctg gcgacaatgg tgtcaaaaga ggctttttct
    29821 gcgccctgct ggcgcataaa ccactccagt tcagcggcta tctcgcgctc gctcatcccc
    29881 gcctgaataa agcggcgaat atgctccgct ccgcgatcgg caatcccaca ggcgaggcgg
    29941 attatctcca cctcctctgg cgttttgatt tgccgcagca catccggcgt ggcgctaacc
    30001 agtttcgcat tgagttcaga cttccagcgg tgcgcggttt cccaactcac ctgctggcct
    30061 tcaaaaccga gcgtttgcaa ctgctcatcg gcaatgattt gattgacgat agtggtaagc
    30121 gtgttcgtcg cgtcaagcaa atgcagctgg tagccttgcg cacgggcttc cacctcaacg
    30181 aaatagcgcg aatctaccag aatgtgcgca ctttcacggc taataaccac atagcctgat
    30241 ccggtggaga tccccagatg cggctgtttg ttctgccgtg aggagagaag caccgcatcc
    30301 agttgttgca ccttaagcca gttgcgcagc gaagcgagta atgtcatctg gattttcctt
    30361 cttacaggct atcgattaac aatttgcctt tgcggaacat catcagacgc aggaacacca
    30421 ccatcagcgc agtaatgatt gcccccagcg cgatccccgc catataaacg cccaggttag
    30481 tgaccagcgg ccatgcccag atagcggatt ccgggaacca ttgcactgcg cccagccaga
    30541 cagctgcggt tgagccgaca atcgcgccga ccatatatga cggaatagcg gtgatggggc
    30601 tttccagcgc aaacggaatc gcaccctcac taatccccat aaaggccagg aacatcgcgg
    30661 tcttaccctg cggataaagt tgagcgttga acaggcgttt gccggttaaa cgacggtcaa
    30721 taatggtcgc cagacccaga ccaatcggcg gaataacgat agcgatagaa cgagcggtga
    30781 ccggtaatac gtggtcagtg gtaaagctga aggcaacgaa acctgcggct ttgttgatcg
    30841 ggccaccaag gtcgattgct gtcgccgcgg cgatccccat tgcgtacatc agcgcgccct
    30901 tctcacctgc ggcagtcagc acagtacgga taccgccgtt gatccagcca ccaaagggag
    30961 tgatgacgta gtacatcgcc agcatgacaa aaatggcgga aagaatcggt agcaaaaatg
    31021 tggttttgaa cgctaacagg aaatccggta actggatttt ctggttcatc catttcacaa
    31081 ggtaaccggc aacgatagaa ataatcagcg caccaatgaa ggtggacggc accggcgaag
    31141 aggtcgccca ttgcatagtg ctgggatcga agttcagcag ttgcgtcggc tgggtagaca
    31201 tcaacccgcc gataaaacca gccgggaacg ccagtttgcc accgatagag ttcgccacaa
    31261 atgcagcaaa catcggaatg gcaaaaccaa acaacacgcc gccgaaggac tgcgacagcc
    31321 aggcaaattt aagtaaggag aggtcgaaac cggagaattt cccgctatta agagcatcca
    31381 tgatgccgat ctcagcagga attttaagcc agctataagc aatcagctgg ctgaaagcga
    31441 ggatcacacc gcccataatt aaggtcggca ccatgcgtga aatccctgac atcacatgct
    31501 gcgggagttc accccagaag ctgcttttag aggcctgcgg attcttaact gccgctgccg
    31561 caccggatgc gccaggcaca acggttgcac tgcgtttttt aatggccata acaattatcc
    31621 ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc gatttctttg atgatgcccg
    31681 cagcgttttt aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat tcgaaacgtt
    31741 cgttatcttc cggggtaact gccacggaat ggatgatgat ggtcgcttcg gcgatatcct
    31801 gcgccgtcag gcgattctgg atgccgtccg cgccctgggt ttcaattttc acttcataac
    31861 cggcttctac cgccgcttct tccagcacct gagcggccat aaaagtgtga gctaggccca
    31921 ttgggcaggc acataaggca atcagtttct tactcatcgt taattcctcg ttcagtgata
    31981 gttgacgagt gaaattatga acgtgcaaaa caaatcgccg ttaccagaca aataatgcat
    32041 ttactggaga attaatcccg ctgtacggaa gtgtgacgca ggtcggcctt gtgaggggac
    32101 acaaagaaaa attgaattaa ctgtatgatt taaaagatta tcgggagagt tacctcccga
    32161 tataacagga aggatttaca gaatgtgacc taaggtctgg cgtaaatgtg caccggaacc
    32221 gagaagaccc ggatagtcat ggacgatgag atacaccgga atatcatgga catattcttt
    32281 aaagcgccct ttatcttcaa atgcggcacg gaaaccggag gctttgaaga actcaaggaa
    32341 gcgcggcacg ataccgcccg caataaacac gccgccaaat gtcccgagat tgagcgccag
    32401 attgccgcca aaacggccca taatgacgca aaacagcgac aatgcgcggc ggcaatcggt
    32461 gcagctgtca gccagcgcgc gttcggtaat atcttttggc ttgagatttt ctggcaggcg
    32521 gttgtcagct ttcacaattg cgcgatacaa attcaccagc ccagggccag aaagcacgcg
    32581 ctccgccgaa acatgaccaa tttccgcacg caatatttcg aggataatgg cctcttcttc
    32641 actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc
    32701 gacatggacc agatgcgcaa ccccaagccc cgttccggca ccgtaaaccg caataggctt
    32761 accttcgacc ggttctgcgc caccaaactg aatcagatgc tcttttttca gcatcgggat
    32821 cgccatcgat acagcggtaa aatcgttaat aatttccaga tggctaaaac cgagattctt
    32881 tttcatttcg gcaattgaga acgcccaggt atggttggtc attgccaccc agtcaccggt
    32941 aattgggcaa gcgatggcaa tacagccgtc tttcacctcg accttatgtt cttcaagata
    33001 aacgcgaatg accgcttcga ggctggggta atcaagccct gaataggtct tagcctgcga
    33061 gatttcacca ctggcaatat cacacagagc aagacgtgcg ttggtgccgc ccacatcacc
    33121 gactaatgca tactttgtca ttcttcaact gctccgctaa agtcaaaata attctttctc
    33181 acactgtaaa tacctggggg cataacaaca acgccggaaa ggcaggctcc ctgtaaatat
    33241 cgatctgggt cacacaatta ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc
    33301 aagctgggca aactaagtat ctgaccccgc ataaggaata gaacatgctc catccgcgag
    33361 ccagaaccat gttgttatta tcgctccccg ccgtggcaat tgggattgcg tccagtctta
    33421 ttctgattgt ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg caacgactgc
    33481 cgggaactct ggggatagcc caggattcac ccctctggat catcggtgta ttaacgctaa
    33541 cgggtattgc ggtggggttg gttatccgtt tcagccaggg tcatgccgga ccagaccccg
    33601 cctgtgaacc gctgatcggc gcaccggttc cgccctctgc gctacctgga cttatcgtag
    33661 cattaattct cggtcttgct ggcggcgtca gcctggggcc ggaacatccg atcatgaccg
    33721 tcaatatcgc ccttgccgtc gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg
    33781 agtggactat tttagcctct gccggaacca tcggcgcgct gtttggtaca cctgttgcgg
    33841 cggcgttgat attttcgcaa accttaaatg gcagtagtga agttccgcta tgggatcgtc
    33901 tttttgcgcc gttaatggcg gcagcagctg gtgcacttac taccggatta tttttccatc
    33961 cccatttttc actgcccatt gcgcattacg gacagatgga aatgaccgat attctcagcg
    34021 gtgcaattgt cgcggcgatt gccatcgcag cagggatggt cgccgtatgg tgcttaccac
    34081 ggttgcacgc gatgatgaat caaatgaaaa atccggtgct cgtgctgggt attggcggat
    34141 ttattctcgg aattctgggg gttattggtg gaccagtttc gctgtttaaa gggctggatg
    34201 agatgcagca gatggtggca aatcaggctt tcagcaccag cgattacttt ttgctggcgg
    34261 taattaaact tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct
    34321 tcccggcagt gtttgtcggc gtggcattag ggttgatgct gcatgagcac gttcccgccg
    34381 ttccagcggc aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg
    34441 atggctggtt aagtcttttt atggcggcag tcgttgtacc caataccaca ttgctaccgc
    34501 tgctctgtat cgtcatgctt ccggcatggc tgttattagc aggtaagccg atgatgatgg
    34561 tcaatcgtcc gaagcaacag ccaccccacg ataatgttta gcaaaaaatg ctctccttta
    34621 tgatttaaga gttatggcga tgatacgggg ctttacctcc ccgtaatatt gcgttaacag
    34681 gccgctgacg gcttcgatcg tgaaggagaa taacaatgtt caggtcactg tttctggcgg
    34741 ccgccctgat ggcatttacc ccgcttgcag caaatgcagg tgaaatcacc ctactgccat
    34801 cgatcaaatt acaaattggc gatcgcgatc attacggtaa ttactgggac ggtggtcact
    34861 ggcgagatcg tgattactgg catcgcaatt atgaatggcg caaaaaccgc tggtggcgtc
    34921 atgataatgg ctatcatcgt ggctgggata agcgtaaagc atatgagcgt ggctatcgtg
    34981 aaggctggcg cgatcgtgac gatcatcgcg gaaaaggccg cggacatggg caccgccatt
    35041 aaacgcgtcc tccaatggag cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct
    35101 acaaaccggc tctttaacgc gtagggcgga taaggcgttt acgccgcatc cgccaatagt
    35161 gccagatgcg acgctcattc aactacaatc ccagcgccgt ccccaccaac aaccagatat
    35221 tcagcgccac gaccagcact acaatcaccc agcctgtctg ttttacgcgt ttgctgttca
    35281 ccagatcgcc catcaacttg ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg
    35341 cgataccaaa acttaacagc acctgactca taaccagaat ccgtgtcgga tctaatccca
    35401 tcagaatgac aataaatgac ggcaacatgg tgactgtacg acgcacccac agcgggatat
    35461 ggaagcgaat gaatccctgc atcaccacct gccccgccag tgtccccacc accgttgagg
    35521 acagtccggc agcaaccaga cttaacccaa agaccgttgc cgcagcatgg cttaacagcg
    35581 gttgcagcgt cagataagcc tcatcaagat cggcaacacc agtatgacca gaaaagtgga
    35641 acgccgccgc agctgtagcc atcatcacca gattgacaaa accggcaatc gtcatggcga
    35701 tagccacatc ccatttggtg gcggaataac gttgttgacg cgaaccgcca tgtaaatgct
    35761 gagtgagcga agagtgcaaa taaatcacat gcggcataat cgtcgcccct aacacgcctg
    35821 ctgccaggaa gaccgcctcc gaagtaggta aactcgggat caccattcct ttacccagct
    35881 gcgccaggtt aggctgggag aaaatcaact cgacaatgta agccgcggca acaaacaaca
    35941 gtaacccgcc aatcactttc tccagcggtt tttgcccgcg acgttgcagc attaaaatca
    36001 ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa cgaaacacca agaatgagtt
    36061 taaaaccgat cgccgcacca ataaattccg ccaggtcggt tgccatcgca ataatttctg
    36121 cctgaaccca atagaaccac actacgggac gcggatagtg atcacgaatc tgctccgcca
    36181 gatttttacc ggtggcaatc cctagtttgg cagagaggat ctgaatcagc atcgccatca
    36241 ggttggccca aacgacaacc cacagtagct gatagccgaa gctagcaccc gcctgaatat
    36301 tggtcgcaaa gttaccggga tcgatataac caatcgccgc aatgaacgca ggtcccatta
    36361 atgcgagcct catcttgcgc gccgcccgtc cgctgctact ctcaacgcga tagttcgtca
    36421 tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc tatgctaaac gagaatgatt
    36481 atcaaattca tttaaatgga ttgtggtgat ttctctgata gaccaagatt atgattacga
    36541 aaagattgat gacttgaatg ttatggttat gtttaatgtt agcacattta cataactttc
    36601 agcttccata cacaacatag cagaaatgta tgacagatca ctatttttga agcctgtcac
    36661 aggacgtcat tatagtgtgt gtcagatctc gttttcctta accatgttac atagaatgtg
    36721 cacggaaatt taacctgcct catatttgga gcaaatatgg accgcgtcct tcattttgta
    36781 ctggcacttg ccgttgttgt gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt
    36841 cgtatccgtt atgttattca actgcttgtt atcgaagtgt tactggcgtg gttcttcctg
    36901 aactccgacg ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc
    36961 ggatttgcca acgaagggac taacttcgtc tttggtagca tgaatgatca aggcctggca
    37021 ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct ctgcactgat cggtattctc
    37081 cagcacattc gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct ctccaaagtc
    37141 aacggtatgg gcaaactgga atcctttaac gccgtcagct ccctgattct gggtcagtca
    37201 gaaaacttta ttgcctataa agatatcctc ggcaaaatct cccgtaatcg tatgtacacc
    37261 atggctgcca cggcaatgtc caccgtgtcg atgtccatcg ttggtgcata catgaccatg
    37321 ctggaaccga aatacgtcgt tgctgcgctg gtactgaaca tgttcagcac ctttatcgtg
    37381 ctgtcgctga tcaatcctta ccgtgttgat gccagtgaag aaaacatcca gatgtccaac
    37441 ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat acattctggc aggtttcaaa
    37501 gttgccatta tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc cgcgctgaac
    37561 gcactgtttg ccaccgttac tggctggttt ggctacagca tctccttcca gggcatcctg
    37621 ggctacatct tctatccgat tgcatgggtg atgggtgttc cttccagtga agcactgcaa
    37681 gtgggcagta tcatggcgac caaactggtt tccaacgagt tcgttgcgat gatggatctg
    37741 cagaaaattg cttccacgct ctctccgcgt gctgaaggca tcatctctgt gttcctagtt
    37801 tccttcgcta acttctcttc aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa
    37861 gagcaaggta acgtggtttc tcgcttcggt ctgaaactgg tttacggctc taccctggtg
    37921 agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaaa accatacata aaaaagccgg
    37981 ggatcattcc ccggcttttt tacgcctgtt aatcagctaa tggctgcggg cgaccaatca
    38041 aatacccttg cagatattgc accccgagct tatgcaatag cgcctgctgc tgttgcgtct
    38101 cgacaaactc cgcgaccaca ctcaatgact tcgctttcgc cagatcggta attgatctca
    38161 caatcatcgc atccagcgta ttcgtgacaa tatctttcac aaagacgcca tcaattttga
    38221 tgatatcagc ctgcaaacgc tttaaccgtt cgtagttggc atatccggtg ccaaagtcat
    38281 caatcgcaat ccggaaacca aacttatgca gctgctcgat gttgtacatg ctgctttctg
    38341 cgttagaaaa cgcctgctcc tcggtgatct caagaatgac cgcctgcggg gagatgtgat
    38401 aacgcttaaa cagacgaata atccgcccgg caatattctt ttgcagcagc gtgagcggca
    38461 ttaaattgac tgaaaagcgc ggtccttttt tgtcgcaagg gtgtgttgcc agccacttca
    38521 acaaggattc cagcacttgc aaatcaaaac gcgcgctaag gttaaactga gcaataaggg
    38581 gcagaaactt atccggggtc ataatgccgc cgtcatattt cagtcgcgcg aggatctcat
    38641 cataaccttc accctctttg ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat
    38701 ctaacgccgt gcgaatggta ttcagcaata gcacctgttt agtggtctgc ccggaaacca
    38761 tctcctctct gctatccagc gccagcacat gatgatgtgc gcaggattgc tccgccagcc
    38821 agcttaactg ccccaacaag ggttgcaggg tttcctgatt tccatcaaaa cgcccccagg
    38881 cagcaccata gcccatatcc agcccggtat tgttccagtg aatttgccga ctattcagga
    38941 tgttaaccat atgctggagt cgcccttccg tttccggccc gctcagcact aacagcagtt
    39001 cactacccgg caattgatac aacttttcgt tttcctgcat caacggcagc agcgtacggc
    39061 aaattgagcg gatacagtga acgcgcatca ttaagccgta atgacgactc ataaactcaa
    39121 gattatcaat gcgcaggcag caaaaactct tgcccgcctc ttgctccggc gcttgttcca
    39181 acgcacgaaa gttgggtagg agcgttaacg gatctgttag cgcctgcaaa tgccagcggc
    39241 gattaagcca ttcactgcga tgataaatgc gcaccatata gagcaggcaa acgctaaagg
    39301 aaatcagcac cgcgagaata aatgccagcg aatattcggt ttccacccct tgcaaaaagt
    39361 tctggttgta attcagaagg caaagcgttg aaacagccca ggtgagattt aaaaacggat
    39421 agcgaagatt accgaccccg agggtaaaga tgatgaagaa aacgggtacc aggtaaccgg
    39481 caataaagtc gttttcataa ggtgtgcaca acagaagtag cagcacgctt aacgctgcca
    39541 gccagcttaa ggtaaatgcg agtgagatag tagaaaagca tgttgtaaat cagcactgcg
    39601 gtgaaaaggc ttagtaaatc aacgaccgtg aaaatggcat ccgcatcgcc gaaaaaggtg
    39661 gatatcttta gcgggaaatc aaagaagctc cccacaagat acatgctgca tttgatgccg
    39721 atcggcgtca ccaaaccaag ccagaacaga cgttgccaga catatcggct ggtcaatcca
    39781 taacgccaac gcgtccccag ctgccagcgt aatattgcgc aggcacacaa cacgacaaac
    39841 gtctggctgc cgagcatgac cgcagtctgt aaaaaagaga gatgaaagtt ccacagattg
    39901 gtgcaaaaca tcccgcataa aacgggaacg acaccgcgcc agccaaagat aaacaagatt
    39961 gacagcatta cgcacagtgg catccacgcc agaaagatat aactggaatg gataatggca
    40021 agtggcgaaa taaaacggga tagctgaata agtaccacgg tgagcgtaaa cgctagtgtg
    40081 aatatcttga tattttttat caggttatgc tccacaaaca tgagtacact actctcactg
    40141 atcggggttt tcaggcacac aaataataag tttattcgca tcgcgaagca aacaggggcc
    40201 agcctgttgc cgatttttca tccaatgatg ctttcaccag gcggcgctaa cccggacgta
    40261 gactgtatta caaaagcggc aaaaagcaga gacaaaaaac ccccgctttg cagcgagggt
    40321 tggaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg
    40381 ctctcccagc tgagctatag ccccacgatg cgtttacgta ccaaatttgc tgggtgcaaa
    40441 atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc
    40501 ccagctgagc tatagccccg tcacgtaaag cttgtcgagt tgacgggcgg catcatatga
    40561 attccgcccg aatgtgtcaa cggcaaattg caacgtgtag tttcaatcgc tgaaaaatca
    40621 ggcaaatgaa caattttgga aaaccgctcg cattcagtag ttattcatgt cacggtttcc
    40681 tgtaaagtgg tgttataaaa tgaactacta atagacccac atacattcag ggaattgtta
    40741 tgttcaagga gaggatgaca ccagatgaac ttgccagact gaccggctat agccgccaga
    40801 ccattaataa atgggtacgc aaggaaggct ggacaacgtc accaaaacct ggcgtccagg
    40861 gtggcaaagc ccgactggtt cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg
    40921 aacgtccaga aggtcaggga gaagcgcctg ccctttctgg tgatgcgccg cttgaagtat
    40981 tgctggtgac actggcgaaa gagatgacgc cagttgaaca aaaacagttt acatccttgc
    41041 ttctgcggga agggattatc ggattgttac aacgcttagg gattcgcgat agcaaataat
    41101 atgaaaagat tacgcaataa aatgaccacc gaagaactgg ctgaatgcct cggtgtggct
    41161 aaacaaactg ttaaccgttg gaccagagaa aaaggctgga aaacggaaaa atttcccggc
    41221 gtgaaaggcg gtcgtgccag acttattctg gtcgatacgc aagtttgcga gtttattcag
    41281 aacacgccag ccttccataa cacaccaatg ttgatggaag ctgaagagag aattgctgaa
    41341 tacgctcctg gagcacgcgc cccggcgtat cgacaaatca tcaacgcgat agataatatg
    41401 acagacattg agcaggaaaa agtcgcgcaa tttttgtcac gtgaaggaat tcgcaatttc
    41461 ctcgcccgtc tcgatattga cgaatcagca taaataaaaa acggcaggat attatctcct
    41521 gccgtttatc tttttacacg ctaattactg ctgattttcg cgttcagcaa taaaatccag
    41581 cgctttgttg atacgctcga tactgcgggt cttaccaatt gcgtgaacgg taacatccag
    41641 tgctggagac tgccccgcac cggttacggc gacacgcagc ggcataccaa ctttacccat
    41701 acccacttcc agctcatccg ccgtcgcctg aatagcgtga tgaacgtttt cagcggtcca
    41761 gtcagtaatc gcggccagtt tgtcacgaac cacttccagc ggctgacgcg ctaccggacg
    41821 cagatgtttt ttcgccgcat cggcatcgaa ctcagcaaaa tcttcgtaga aataacggca
    41881 gctctgtgcc atctctttca gcgtcttgca gcgttcgccc agcagtttca ccagatcagc
    41941 cagctgcggg ccgttacggg tatcgatatt ttcctgctca atgtgccact gtaagtgagt
    42001 agcaacatac tccggcggca gcgcgttaat gtagtgatgg ttcagccaca gcagcttgtc
    42061 ggtgttgaac gcactggcag atttgctgac ggcattcaaa gtgaagtatt tgatcatctc
    42121 ttcacgagtg aagatttcct gatcgccgtg ggaccagccc agacgcacca gatagttcag
    42181 cagtgcttct ggcaaataac cgtcatcacg atactgcatt acgctgactg ccccgtgacg
    42241 tttggacagt tttttaccgt catcgccatt gatcatagaa acgtgcgcgt aaaccggcac
    42301 cggcgctttc agggccttaa gaatgttgat ctggcgcggc gtgttgttga tatggtcttc
    42361 gccacggata acgtgggtga tttccatatc ccagtcatcg acaaccacac agaagttata
    42421 ggttggggaa ccatcggtac ggcggataat aagatcgtcc agttcctggt tgctgaactc
    42481 gatcggacca cggatctgat cgtcaaaaac aacagaacct tcctgcgggt tagcaaaacg
    42541 tacaacacac ggttcatcat cagcatgatg ctcatggctg tggcggcagc gaccgtcata
    42601 acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc gcttccaggc gctctttaga
    42661 gcaatagcat ttataagcag tgccctcttc cagcatctga tcgatcaccg cgttgtagcg
    42721 atcaaaacgt ttggtctggt agtacggacc ttcatcccac tccaggctca gccagttcat
    42781 gccatccata atggcttcga tagcttccgg cgtggaacgc tcaagatcgg tgtcttcaat
    42841 acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc caggagtaaa gagcagtacg
    42901 cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg aagcgagttt tgattttcat
    42961 gaaatggcct tacgtttaga aagatgccga caaccggcaa atcctggaaa aattgagtgg
    43021 gcgatattct atcactcccg cctgattcct caatgtagtt cgggtttcta ccttacgcat
    43081 tattgttttc gttgacaaat tgcgcaacac ggtctgtttt gcgatcatct tgcttaaatt
    43141 tacgacgaac gaacaatttc tttagaaaat gcgttgactc attttgaact ctccctataa
    43201 tgcgactcca cacagcgggg gtgattagct cagctgggag agcacctccc ttacaaggag
    43261 ggggtcggcg gttcgatccc gtcatcaccc accaactact ttatgtagtc tccgccgtgt
    43321 agcaagaaat tgagaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag
    43381 ggggtcggcg gttcgatacc gtcatcaccc accacttcgg gtcgttagct cagttggtag
    43441 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc t
//
LOCUS       DBKELA010000022        43057 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.022, whole genome shotgun sequence.
ACCESSION   DBKELA010000022 DBKELA010000000
VERSION     DBKELA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.022"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(33..374)
                     /gene="hypA"
                     /locus_tag="ACUQR0_001184"
     CDS             complement(33..374)
                     /gene="hypA"
                     /locus_tag="ACUQR0_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HHV9108311.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(367..855)
                     /gene="hybE"
                     /locus_tag="ACUQR0_001185"
     CDS             complement(367..855)
                     /gene="hybE"
                     /locus_tag="ACUQR0_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HHV9108312.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(848..1342)
                     /locus_tag="ACUQR0_001186"
     CDS             complement(848..1342)
                     /locus_tag="ACUQR0_001186"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HHV9108313.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1342..3045)
                     /gene="hybC"
                     /locus_tag="ACUQR0_001187"
     CDS             complement(1342..3045)
                     /gene="hybC"
                     /locus_tag="ACUQR0_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HHV9108314.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3042..4220)
                     /gene="hybB"
                     /locus_tag="ACUQR0_001188"
     CDS             complement(3042..4220)
                     /gene="hybB"
                     /locus_tag="ACUQR0_001188"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HHV9108315.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4210..5196)
                     /gene="hybA"
                     /locus_tag="ACUQR0_001189"
     CDS             complement(4210..5196)
                     /gene="hybA"
                     /locus_tag="ACUQR0_001189"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HHV9108316.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5199..6317)
                     /gene="hybO"
                     /locus_tag="ACUQR0_001190"
     CDS             complement(5199..6317)
                     /gene="hybO"
                     /locus_tag="ACUQR0_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HHV9108317.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6506..6793)
                     /gene="yghW"
                     /locus_tag="ACUQR0_001191"
     CDS             complement(6506..6793)
                     /gene="yghW"
                     /locus_tag="ACUQR0_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HHV9108318.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(6912..7799)
                     /gene="yghX"
                     /locus_tag="ACUQR0_001192"
     CDS             complement(6912..7799)
                     /gene="yghX"
                     /locus_tag="ACUQR0_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HHV9108319.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7956..8996
                     /gene="gpr"
                     /locus_tag="ACUQR0_001193"
     CDS             7956..8996
                     /gene="gpr"
                     /locus_tag="ACUQR0_001193"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HHV9108320.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9036..9530)
                     /locus_tag="ACUQR0_001194"
     CDS             complement(9036..9530)
                     /locus_tag="ACUQR0_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HHV9108321.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9721..10605
                     /gene="yghA"
                     /locus_tag="ACUQR0_001195"
     CDS             9721..10605
                     /gene="yghA"
                     /locus_tag="ACUQR0_001195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HHV9108322.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(10877..11302)
                     /gene="exbD"
                     /locus_tag="ACUQR0_001196"
     CDS             complement(10877..11302)
                     /gene="exbD"
                     /locus_tag="ACUQR0_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HHV9108323.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11309..12043)
                     /gene="exbB"
                     /locus_tag="ACUQR0_001197"
     CDS             complement(11309..12043)
                     /gene="exbB"
                     /locus_tag="ACUQR0_001197"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HHV9108324.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12295..13482
                     /gene="metC"
                     /locus_tag="ACUQR0_001198"
     CDS             12295..13482
                     /gene="metC"
                     /locus_tag="ACUQR0_001198"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HHV9108325.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13622..14281
                     /gene="yghB"
                     /locus_tag="ACUQR0_001199"
     CDS             13622..14281
                     /gene="yghB"
                     /locus_tag="ACUQR0_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HHV9108326.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14321..15220)
                     /gene="yqhC"
                     /locus_tag="ACUQR0_001200"
     CDS             complement(14321..15220)
                     /gene="yqhC"
                     /locus_tag="ACUQR0_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HHV9108327.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15414..16577
                     /gene="yqhD"
                     /locus_tag="ACUQR0_001201"
     CDS             15414..16577
                     /gene="yqhD"
                     /locus_tag="ACUQR0_001201"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HHV9108328.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16682..17509
                     /gene="dkgA"
                     /locus_tag="ACUQR0_001202"
     CDS             16682..17509
                     /gene="dkgA"
                     /locus_tag="ACUQR0_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HHV9108329.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            17709..18635
                     /locus_tag="ACUQR0_001203"
     CDS             17709..18635
                     /locus_tag="ACUQR0_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HHV9108330.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18686..18943
                     /gene="yqhH"
                     /locus_tag="ACUQR0_001204"
     CDS             18686..18943
                     /gene="yqhH"
                     /locus_tag="ACUQR0_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HHV9108331.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(18986..21205)
                     /locus_tag="ACUQR0_001205"
     CDS             complement(18986..21205)
                     /locus_tag="ACUQR0_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HHV9108332.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21316..22728)
                     /gene="ftsP"
                     /locus_tag="ACUQR0_001206"
     CDS             complement(21316..22728)
                     /gene="ftsP"
                     /locus_tag="ACUQR0_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HHV9108333.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(22803..23540)
                     /gene="plsC"
                     /locus_tag="ACUQR0_001207"
                     /gene_synonym="parF"
     CDS             complement(22803..23540)
                     /gene="plsC"
                     /locus_tag="ACUQR0_001207"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HHV9108334.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(23774..26032)
                     /gene="parC"
                     /locus_tag="ACUQR0_001208"
     CDS             complement(23774..26032)
                     /gene="parC"
                     /locus_tag="ACUQR0_001208"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HHV9108335.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26578..27060)
                     /locus_tag="ACUQR0_001209"
     CDS             complement(26578..27060)
                     /locus_tag="ACUQR0_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHV9108336.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27113..27505)
                     /gene="ygiW"
                     /locus_tag="ACUQR0_001210"
     CDS             complement(27113..27505)
                     /gene="ygiW"
                     /locus_tag="ACUQR0_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HHV9108337.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27657..28316
                     /gene="qseB"
                     /locus_tag="ACUQR0_001211"
     CDS             27657..28316
                     /gene="qseB"
                     /locus_tag="ACUQR0_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HHV9108338.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28313..29586
                     /gene="qseC"
                     /locus_tag="ACUQR0_001212"
                     /pseudo
     CDS             28313..29586
                     /gene="qseC"
                     /locus_tag="ACUQR0_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
     gene            complement(29632..29964)
                     /locus_tag="ACUQR0_001213"
     CDS             complement(29632..29964)
                     /locus_tag="ACUQR0_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HHV9108339.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLMPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            30283..30864
                     /gene="mdaB"
                     /locus_tag="ACUQR0_001214"
     CDS             30283..30864
                     /gene="mdaB"
                     /locus_tag="ACUQR0_001214"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HHV9108340.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            30895..31209
                     /locus_tag="ACUQR0_001215"
     CDS             30895..31209
                     /locus_tag="ACUQR0_001215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HHV9108341.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(31257..33149)
                     /gene="parE"
                     /locus_tag="ACUQR0_001216"
     CDS             complement(31257..33149)
                     /gene="parE"
                     /locus_tag="ACUQR0_001216"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HHV9108342.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(33178..33759)
                     /gene="yqiA"
                     /locus_tag="ACUQR0_001217"
     CDS             complement(33178..33759)
                     /gene="yqiA"
                     /locus_tag="ACUQR0_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HHV9108343.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(33759..34586)
                     /gene="cpdA"
                     /locus_tag="ACUQR0_001218"
     CDS             complement(33759..34586)
                     /gene="cpdA"
                     /locus_tag="ACUQR0_001218"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HHV9108344.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(34611..35033)
                     /locus_tag="ACUQR0_001219"
     CDS             complement(34611..35033)
                     /locus_tag="ACUQR0_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HHV9108345.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(35034..35663)
                     /gene="nudF"
                     /locus_tag="ACUQR0_001220"
     CDS             complement(35034..35663)
                     /gene="nudF"
                     /locus_tag="ACUQR0_001220"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HHV9108346.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            35868..37349
                     /gene="tolC"
                     /locus_tag="ACUQR0_001221"
     CDS             35868..37349
                     /gene="tolC"
                     /locus_tag="ACUQR0_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HHV9108347.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            37497..38168
                     /locus_tag="ACUQR0_001222"
     CDS             37497..38168
                     /locus_tag="ACUQR0_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HHV9108348.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            38174..39334
                     /locus_tag="ACUQR0_001223"
     CDS             38174..39334
                     /locus_tag="ACUQR0_001223"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HHV9108349.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(39372..40160)
                     /gene="ygiD"
                     /locus_tag="ACUQR0_001224"
     CDS             complement(39372..40160)
                     /gene="ygiD"
                     /locus_tag="ACUQR0_001224"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HHV9108350.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            40303..41076
                     /gene="zupT"
                     /locus_tag="ACUQR0_001225"
     CDS             40303..41076
                     /gene="zupT"
                     /locus_tag="ACUQR0_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HHV9108351.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(41134..41304)
                     /gene="yqiD"
                     /locus_tag="ACUQR0_001226"
     CDS             complement(41134..41304)
                     /gene="yqiD"
                     /locus_tag="ACUQR0_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HHV9108352.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(41565..42218)
                     /gene="ribB"
                     /locus_tag="ACUQR0_001227"
     CDS             complement(41565..42218)
                     /gene="ribB"
                     /locus_tag="ACUQR0_001227"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HHV9108353.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(42322..42470)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            42592..42882
                     /gene="ubiK"
                     /locus_tag="ACUQR0_001228"
                     /gene_synonym="yqiC"
     CDS             42592..42882
                     /gene="ubiK"
                     /locus_tag="ACUQR0_001228"
                     /gene_synonym="yqiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HHV9108354.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        10754 a        11475 c        10716 g        10112 t
ORIGIN      
        1 gtccgtgcgc tttttaggcg ttgtcctcag tcttattcaa cttcaatact tttgacgatc
       61 agcgaatcgc cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg
      121 tgctgatgaa tctccaccac ctggctgcaa tcccagcacc aggcctgggc gggtttatag
      181 acgatatgta aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa
      241 cggacggcgc tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg
      301 cgcttaacat cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaagc
      361 gacaactcat gcattttcgc tcctcctgcg gccaaacagc agggcgcgac gccctgcgtg
      421 tggtacatcc ggattggtga ctggcaacga aaggatcatt cgtgcgcagt catccgtcag
      481 gcgttggcct tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag
      541 gtattgcgaa acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg
      601 cagaccaatt ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat
      661 aactgcactc agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt
      721 aaaatcagaa acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg
      781 ccgggcaatt tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc
      841 tatctcttca gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg
      901 cagccagaac ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga
      961 tgtgtggctc cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac
     1021 cggtgaagcg cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt
     1081 tggtaaacaa cgccggaact tcttcatccc gcaggatcat catcgttccc ggagtgttct
     1141 ttttcgacac aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca
     1201 gctccattcc cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt
     1261 gctctaacgc ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa atattgccga
     1321 cccctaagac taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc
     1381 atccactacg tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac
     1441 ttccagcggt ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc
     1501 gacgtcatca ttgaagttac gcggaccaga gttccaggtt gatggaacaa ccgcctggta
     1561 gttgctgatg ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc
     1621 gaggaagcca acacctttga actcacccgt tgccggaatg ttcggcttca caaaggtggt
     1681 gtgatcgcct ttgccgatat tggtgatcag tgcactgtat tggttttgca ggatatcctg
     1741 caattcacag cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgtgc
     1801 cacttccagc gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt
     1861 ctgggtagat tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc
     1921 taccgttttg ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca
     1981 accatcataa gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt cgtctttata
     2041 ccaggagtgc ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt
     2101 gatcggacga tacgaggaca gatccgcatt ctcaatgtag cctcccggga acaggaagct
     2161 gccgtttttg ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc
     2221 gcgcgtcagc cattccgggt agaacgcggc gataaccgcg gtatcaacct tataaacctg
     2281 ctcaacaaag tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt
     2341 caacacgccc aacccgtcga ggttgattgg gttcgcgaca ccacccactg ccaggttctg
     2401 aatgtgcggc gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc
     2461 cagcgcctgc aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc
     2521 cgggtgaccc cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt
     2581 gatcttgttc tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc
     2641 tttcagcatt tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca
     2701 gtccagcgcc gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag
     2761 aatgatgtta cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc
     2821 cgcacgaacg gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat
     2881 catccatgca tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt
     2941 accggaagcc catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg
     3001 cccctcaata cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca
     3061 tgacgattat gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg
     3121 atgtaagcgc aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc
     3181 gggaagtagg cgtaaccgcc gcccgggttg aatgccacca acgaataggt cagacgccag
     3241 gttgcacaac ctaacagtgc gctcagtgct gacaggaaca gcatgcggga atcattacgc
     3301 agcttcgcca cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac
     3361 atcacggagt agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg
     3421 ccaaagcgca gcccgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg
     3481 aacaggctct tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca
     3541 aagatgacaa tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt
     3601 tcatagctct gccagagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt
     3661 gaagactggt gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg
     3721 ttcagacgtt gtagcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc
     3781 agagccatca cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc
     3841 acgttgaagt gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata
     3901 gtgatcgaca agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc
     3961 agcggatggt attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca
     4021 caggcaaagc cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg
     4081 ccgttcaggt cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata
     4141 cagatgacga ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc
     4201 ggttgtggat catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg
     4261 acgaaccagc acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag
     4321 ggtgtgttga atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc
     4381 gagattttca taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc
     4441 gccgtacaga tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag
     4501 cgtctgacgc ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc
     4561 cgccatcagc tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca
     4621 gccaggcaga ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt
     4681 atgcagcgca ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac
     4741 catgcagtaa cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg
     4801 atcttttttc agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat
     4861 acactgtttc ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc
     4921 gctggtccac acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca
     4981 ggtctgttcc ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca
     5041 agcctggcag cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg
     5101 cgggcggttt tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc
     5161 gcaggaggct gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg
     5221 ttatctttct tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc
     5281 agcccaacca caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta
     5341 gcgttaacat ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg
     5401 atgcctttat ggaagccgat acctttttcg ttacagccat agcacgggtg accaatcgcc
     5461 accggccaca caccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct
     5521 ggccctttac agccgaggtg gtacaggcac cagccttcgc ggtggccttc atcaccgaac
     5581 tctttggcaa aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga
     5641 cggccatagg cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg
     5701 atgatgtgcg caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg
     5761 ataacggttt tgcctggcag aacttcttgc aagctgactg cgccagttgg gttaactcca
     5821 gctgcggcaa caccgcccca cgcggagcag gaaccaatag cgataatggc tgctgagcct
     5881 tccgccgctt tgcggatgtg atccacaatc ggctcaccgg caaccatgca ataaataccg
     5941 ttatctttta atgggatgga accatccacc actaacacat actgcccttt gtacttctcg
     6001 agagcgttat gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga
     6061 tattccagag agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc
     6121 agagattccg tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc
     6181 gggttagtaa ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg
     6241 gctaatgctg cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg
     6301 gtgttatctc cagtcattta tagttattcc gttgcgagga cctggcatat attttgcctc
     6361 aatcgcaaaa tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg
     6421 atatcatgat actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt
     6481 aaagcaagat aatacgtatg tttgatcaat tttcgaggca ataaccggcc ataaaaaagc
     6541 gcaacgtaga acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc
     6601 catagcggcg cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt
     6661 cgtacatccg gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg
     6721 tcacattaac cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa
     6781 aatggttatt catcgttcac ctcattcgtt atatatcgcc atatatagcg atattttagg
     6841 caaagaacga cttttgcgca cgctattttt tcatacttgt ttaagacgta atcccgtaag
     6901 agataaacct atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga
     6961 tcggcggcag atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc
     7021 ggatagatat aagcctcata aaccttatta ttgtctttca acgccgcctc gtaaggaggc
     7081 cagccctcgt tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc
     7141 ttcgccacat cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc
     7201 ggatacgcga cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc
     7261 actttacctg tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc
     7321 atcagtttgg ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga
     7381 taacctccaa cggaacttaa tccgtcaggt gccagggcga tataccccgc cttcgccact
     7441 cgccgtgcca catcttcgat atacggattc agtccacgat tctcatgcac cacccccacg
     7501 gctggcgttt tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga
     7561 ccatttggcg aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc
     7621 tgagtcgcca gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg
     7681 cccaccgcat acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg
     7741 taatagtcgt agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc
     7801 gttgcattcc tcaactggtg tttttttaag caaagcataa gcgcgtgttt ttgcccagtt
     7861 tttcgtcact ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac
     7921 attgtttcga aattgaacgg aggaaaggag aggtcatggt ctggttagcg aatcccgaac
     7981 gttacgggca gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcattat
     8041 cgctcggttt atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc
     8101 tgcgtaaagc gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc
     8161 ctccaggaag cgcagaagag aactttggtc gcctgctgcg cgaggatttt gctgcttatc
     8221 gcgatgaact gattatctct accaaggctg gctacgatat gtggcccggc ccgtacggct
     8281 ctggcggttc acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc
     8341 ttgagtatgt cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa
     8401 ccgcctctgc gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct
     8461 cttactcgcc agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc
     8521 ttttaattca tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc
     8581 tggataccct gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat
     8641 tgctgaccgg aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga
     8701 ataaagttcg tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct
     8761 tattgaatga aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt
     8821 tgctgaaaga tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag
     8881 aggagaacgt gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg
     8941 atcagcatat cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg
     9001 ttaataacgg gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg
     9061 tcaagatagc ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac
     9121 atcagtttgt tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa
     9181 gaaattgcca caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc
     9241 agccacttca gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa
     9301 ccggaaaaca tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc
     9361 aacacgagga tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc
     9421 tcctggaaga acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg
     9481 gcaagcagcc agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt
     9541 cgtgtagtta aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat
     9601 caacatatac gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc
     9661 ttatcaacgc atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg
     9721 atgtctcatt taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa
     9781 caaccgacgc caggcatcca ggcgaagatg acaccgatac cggattgcgg cgagaaaacc
     9841 tatgttggta gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga
     9901 ataggtcgcg ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat
     9961 cttcccgtgg aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc
    10021 aaagccgttc tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac
    10081 gaagcgcaca aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt
    10141 gccattccgg atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac
    10201 gttttcgcgc tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt
    10261 gtcatcacca cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg
    10321 gctacgaagg cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa
    10381 ggtattcggg tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc
    10441 ggacaaacgc aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg
    10501 caaccggcgg aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc
    10561 accgcagaag tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg
    10621 tgaaaagcaa ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta
    10681 tccgcctaca aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc
    10741 gtagcgcatc aggcattgag cacctaatgc ctgatgcgtc gtgaacgcct tatccggcct
    10801 acaaacgcac gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc
    10861 atcaggcaat tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca
    10921 ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt
    10981 tgtccgctcg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg
    11041 taatcattgt ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca
    11101 ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt
    11161 tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca
    11221 acatcacgtc gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt
    11281 tttcgttaag atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa
    11341 ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc aacaatacct
    11401 gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat
    11461 agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg
    11521 cgatacccgg cgcaacgact gccaagttag tggtctgcgt ttgcgcgata ccaataaagc
    11581 tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg
    11641 ttgccagata gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca
    11701 gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca
    11761 gctcattctg cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg
    11821 cgatatcgtt ggcctggttt aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa
    11881 ggcgacgctt ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta
    11941 cagaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg tactgataca
    12001 taccccagac ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg
    12061 caagtcacaa aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca
    12121 ttactatttg catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa
    12181 gtcaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc
    12241 gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg
    12301 gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc
    12361 gcggtaaata gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa
    12421 aagcacgcga cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta
    12481 acccattttt ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta
    12541 tttccctgcg gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat
    12601 catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc
    12661 agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag
    12721 catctgcagc caaacactaa aatcgtgttt ctggaatcgc cgggttccat cactatggaa
    12781 gtccacgacg ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt
    12841 atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat
    12901 gtttctattc aggccgccac caaatatctg gttgggcatt cagatgcgat gattggcact
    12961 gccgtgtgca atgcccgttg ctgggagcag ctacgggaaa atgcctatct gatgggccag
    13021 atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt
    13081 ttgcgtcaac atcatgaaag cagtctgaaa gtggccgaat ggctggcaga acatccgcaa
    13141 gttgcgcgag ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt ctggaaacga
    13201 gactttacag gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa
    13261 gagctggcga actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg
    13321 tatgaatcgt tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc
    13381 gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat
    13441 ctgattgccg atctggacgc cggttttgca cggattgtat aacattgcca cttttggaca
    13501 attttgcaga cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc
    13561 cggacgattc aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc
    13621 catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc
    13681 ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa
    13741 cggcctgctg cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt
    13801 gattgcccaa ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag
    13861 tctgggctgc tggctaagtt atattcaggg gagctggtta gggaatacca aaacggtgaa
    13921 aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg
    13981 ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc
    14041 aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag
    14101 tggattgctg tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt
    14161 cgttaaacgc catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt
    14221 aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg
    14281 acgattttcc ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc
    14341 gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa
    14401 attgcgatgc gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat
    14461 ggatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg
    14521 tgctggtgac agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg
    14581 ccagttgctc gacgctcagg ttttcggtgt atttattctc aattcgtttc agcacgcggc
    14641 taatcagact gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc
    14701 cggtcagcac gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca
    14761 gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg
    14821 tggcggagtt aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt
    14881 ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca
    14941 cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt
    15001 cattggcatc ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga
    15061 gaattatgat gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca
    15121 acaaacgcac atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa
    15181 ctttatccgc cagcaagcga caaatctctt cacgtttcat atcgcgtaat ttcttaggaa
    15241 taatgcggca atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata
    15301 ggcaagacat tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa
    15361 catcaggcag atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca
    15421 actttaatct gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac
    15481 gcgaacaaat tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa
    15541 aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg
    15601 gcggtattga gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg
    15661 aacagaaagt gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat
    15721 ttatcgccgc agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg
    15781 gcggtaaaga gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg
    15841 gttcagaatc caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt
    15901 tccattctgc acatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc
    15961 tgccgccgcg tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt
    16021 atgttaccaa accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga
    16081 cgctaatcga agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca
    16141 acgtcatgtg ggcggcgact caggcgctga acggtttgat tggcactggc gtaccgcagg
    16201 actgggcaac gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc
    16261 aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta
    16321 agctgctgca atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta
    16381 ttgacgccgc gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc
    16441 tctccgacta cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc
    16501 acggcatgac ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat
    16561 acgaagccgc ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag
    16621 acttaagttc acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg
    16681 tatggctaat ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact
    16741 gggcgtctgg caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt
    16801 gggttatcgc tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc
    16861 cctgaaaaat gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga
    16921 cgaccacaag cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta
    16981 tatcgacctc tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg
    17041 gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt
    17101 ccagatccat cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca
    17161 gatcgaactt catccgctga tgcaacaacg ccagctacac gcctggaacg cgacacacaa
    17221 aatccagacc gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa
    17281 agtcattcgc gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg
    17341 gcatctggat agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga
    17401 aaactttgat gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact
    17461 cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc
    17521 tcccggtggc ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt
    17581 cccgcccgtt gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag
    17641 agccggaagc tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg
    17701 aaaaacagat gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg
    17761 cgcagcctcc ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat
    17821 cagatgccag tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta
    17881 ttcaacaggc gcttaccctg aacgacaatc ttacactacc gggcaatatt ggctggctgg
    17941 attatgatcc ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga
    18001 taacccaaac tgacgtcgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg
    18061 ataaagaaaa gaccacacag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg
    18121 atgatattgt cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa
    18181 cgctggccgc tttagcttca ttgcagaaag aacagccaga atcctttgtt gctgaactct
    18241 ttgaacatat tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc
    18301 aggcggttaa tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata
    18361 tgcaaataga acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat
    18421 tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg
    18481 ttcgttttac gctgaccaat ggcaacaatg aagagcaaga gctggtttta cagcggcgcg
    18541 aaggtaagtg ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga
    18601 ttgaggcaaa aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg
    18661 aacaataaat aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct
    18721 ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa
    18781 tgtacaaaca ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc
    18841 acaaatctat gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga
    18901 ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca
    18961 gcatgtcagt tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg
    19021 caccagcatt caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct
    19081 gacgctgagt cgccatcggc gtatgtttag tcaacgccgg acgggtattg cggttctggc
    19141 gacgagcttc acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc
    19201 caatcagatg ctttttgccc atcgcttcca gcgcctgacg gattaacggc cagtttgccg
    19261 gatcgtggta acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga
    19321 agacgccttc gctcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg
    19381 tggttgagtt cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat
    19441 gctttttcag ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcag
    19501 agatgaaata cgggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca
    19561 gctctttaaa gcggtcatag ctgcccatgc ccggcttcat catcttcgac aacggccctt
    19621 cttcggtatg ttccggagca atcttcagat aaccgccgac gtgatgggtc gccaactctt
    19681 tgatatagcg cggatcttcc acggcgatgt cataacgtac gcctgaggcg ataaggattt
    19741 ttttaatgcc tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag
    19801 tgtccatgtg cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag
    19861 cgcgtggcga tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa
    19921 tcacgcccgt aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat
    19981 cttccgaacg gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc
    20041 cgccaaagca gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac
    20101 gggcattgcc ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca
    20161 tctcttcggt agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt
    20221 tttgcatcaa tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat
    20281 gggcgtacag cactttatcg cccttcactt tctcgaaaga aggcagcaat acgtaggttt
    20341 tttcccacgg tttctggcgc ggtggctgca cgattacagc tttcgcttcc tgctttttcg
    20401 gcgcaacagg tttgttatcc gcacacggca aatcttcgcc atacggatgc gggattgggg
    20461 cgattttccc tggggtatca agacgggtgg aatccacacc gctccagcca ggcagcgcct
    20521 cttttacgat aattgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg
    20581 ccagacgatg cactacttcc accagcggac gttcaccgtt accaaacatc agcatgtcgg
    20641 ctttcgaatc taccagaacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg
    20701 tacggcgcag gctagcctca ataccgccga ggatcaccgg cacatctttc cacgcctctt
    20761 tacaacgctg ggtataaacc agcgtggcgc gatccggtcg cttacccgcg acattatctg
    20821 gcgtgtaggc atcatcatga cgtaaacggc gatcggcggt atagcggttg atcatcgaat
    20881 ccatgttgcc agcagtaaca ccaaaaaaca gattcggttt acccagacgc ataaagtcgt
    20941 ctttgctgct ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca
    21001 tacgaccgca aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca
    21061 aaatgatgtc gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa
    21121 acggtgccgg tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg
    21181 gttggatcag ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg
    21241 ccggggatta taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc
    21301 gggcgagtat gaagattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc
    21361 cgccatttcc agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc
    21421 gaaataaaca agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc
    21481 ttccgggaac ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg
    21541 gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt
    21601 gacatcaata cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact
    21661 gatatcgcga ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg
    21721 aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga
    21781 aaccagaata ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc
    21841 accacaggtg atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc
    21901 gcccggtgcc agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc
    21961 agaaatcaca tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga
    22021 cgcgttcagc agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac
    22081 accgttaacc agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg
    22141 cgtaccaaag ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata
    22201 atggttgggg ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag
    22261 gccgttatag acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc
    22321 tgcgttctgg cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc
    22381 cggaccgccc atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc
    22441 tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg
    22501 ccccaggtaa cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca
    22561 gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg
    22621 aacgggtagc ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca
    22681 aagtgcaatc cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg
    22741 taaaactggc taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg
    22801 acttaaactt ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt
    22861 tgttccatta tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga
    22921 ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga
    22981 ttaatcttat tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt
    23041 gccgcgtgaa atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct
    23101 tccgggaaca tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg
    23161 gtgccgtgag ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac
    23221 aactgcccga agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt
    23281 tgcacaatgt tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata
    23341 gcattgccgt agttttcagc atctgcaggt ttgcggcact caactttcag gccgaacagt
    23401 ggcgcaagac ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg
    23461 aaaaggcagt aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata
    23521 agacgaaaga tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca
    23581 cctgaaatca ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg
    23641 caacgcatga ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg
    23701 ctgccggatg acgacttaac gtttcatccg gcgttccttg ctagcgggag gaaacagcgc
    23761 cctccccggc atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct
    23821 caacacgatc gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag
    23881 tgactttctg tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca
    23941 gcgtgctttg cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt
    24001 ctgccgatgg aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcac
    24061 ttaccgggaa catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat
    24121 cttcaatcac caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac
    24181 ccgcacggtt acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg
    24241 aagccatcag cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag
    24301 gcggcaacgt taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa
    24361 tcgggtcgat ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttactct
    24421 tacctttcac cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga
    24481 tatcatggcc tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt
    24541 cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg
    24601 aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat
    24661 tcattttacg ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac
    24721 tctgctcacc gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga
    24781 ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt
    24841 catcttcgtt acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac
    24901 cttccaggat atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca
    24961 cggtatcgcg acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg
    25021 gacgaccatc cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg
    25081 tagcgaagag gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca
    25141 ccagacgggt cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct
    25201 ttttgttgcg catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg
    25261 gtaatgcgct gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg
    25321 aaccacgtcc gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt
    25381 cagtcggata atccggcccc tgcacgatat ccagcagctg atcgagcgta gttttcggct
    25441 ggtcgattaa tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg
    25501 tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag
    25561 gtagcatttt cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct
    25621 gccccagctc gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca
    25681 ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg
    25741 gataacggta agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgctatcgc
    25801 cgtgcggatg gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg
    25861 cgctggcatt caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca
    25921 gaccatcacc aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt
    25981 aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg
    26041 attcctcaac ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc
    26101 tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg
    26161 ataaaggcat caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag
    26221 ggcagcacaa ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt
    26281 cgagaagttg aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa
    26341 gtatacggtt cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa
    26401 gtgcggcttg ctcatggata tccccgagac ggcttacggc gcaccggata ataaatttcg
    26461 cgtatacatg gtattcctta ccggagaaat tataagccca acaacaatac gcctgtgaca
    26521 ttgttcgctc aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca
    26581 cgccaacggc acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt
    26641 ggtgtagtga aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg
    26701 gcgaataatg ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac
    26761 ggcataacgt ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg
    26821 ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc
    26881 gtcccaggcg acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg
    26941 ccacatgata aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa
    27001 catcgccaca gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat
    27061 tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat
    27121 ttactttgcg gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac
    27181 cctgaatctc aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga
    27241 tatcaacatt gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt
    27301 caacgatatt gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta
    27361 cagtcgttac gctgccgttc ggcccctgaa atcctccggc ctgactttgc gttaccgatg
    27421 ggccagaaaa accgccctgc tctgctgcca tcaccggtgc actgcacagg gccattactg
    27481 cgattactgc tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat
    27541 ttaacaagat agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag
    27601 agcgcgttac aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc
    27661 gaattttact gatagaagat gacatgctga ttggtgacgg catcaaaact ggccttagta
    27721 aaatgggttt tagtgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata
    27781 gcgcacctta tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata
    27841 ttttgcgcga atggcgagag aaaggtcagc gtgagccggt actgaacctg accgcgcgtg
    27901 atgcgttagc ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac
    27961 cttttgcgtt gatagaagtc gccgccaagc tggaggctct gatgcgccga accaacggcc
    28021 aggccagcaa cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca
    28081 cgctggctgg cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga
    28141 tgcgtaacgc tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg
    28201 acgaagaggt caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg
    28261 gtagtgattt tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt
    28321 tacccaacgt cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt
    28381 gacctggctg ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt
    28441 gttcgacacc caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat
    28501 caacgcggcg gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga
    28561 cgatgcgctg acctttgcca tctttaccca cgacggcaga atggtcctta atgatggcga
    28621 taacggagaa gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt
    28681 cggtgaagac gatccttggc gttttgtctg gatgacctca cctgatggca aatatcgcat
    28741 cgttgttggc caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca
    28801 attgatcccg tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg
    28861 tcgtgaactc gcgccgctga acaaactggc gctggcacta cgtatgcgtg accctgactc
    28921 ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg cggccacggt tgagtcgcta
    28981 aatcaactgt tcgcccgcac acatgcgatg atggttcgag aacgacgctt tacctccgat
    29041 gcagctcacg aactacgtag cccgttaaca gcgctgaaag tgcaaaccga agttgcgcag
    29101 ctctctgacg atgatccgca ggcgcggaaa aaagcactgc tccaattaca ttccgggatc
    29161 gatcgcgcta ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat
    29221 aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tacaatcgtc ggtgatggat
    29281 atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc
    29341 atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac
    29401 ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta tcgcccaccc
    29461 ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat cgccaagctg
    29521 catgacatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc gaaggtaagc
    29581 tggtaacttc tctgtctatg agagccgtta aaacgactct catagatttt actaatagca
    29641 aaatataaac cgtccccaaa aaagccacca accacaaacc agacaaataa cgagcgagaa
    29701 tacatatagc caaaactcga acatttcccg cataaagagt tttcttaaga taagaataat
    29761 aagtggcata agaagaaaaa atgctgcaat cattgcttgc aatatacgat cttcgcctga
    29821 aacggaaggt atactgcaaa tattgcctcc cgactcaagc atccagtcat acttattgaa
    29881 agcaaaaaaa gaatctatta accaataagt aagcattata tatagcaatg cttcaaatat
    29941 tgttttaatt ttctgcttta acatatatca gtacgctcat aaaaatccat ttataattat
    30001 caacatgaca cgttgatatt ttcatctttt acttattcaa agccatattt tcaatcccca
    30061 aaaaaaccga accaaacgtg taaattattc cggtgcgagt aagaattatg aggaatggct
    30121 atcagtattg tcattttcag aaaatattta tcctgcatcg gtgagtcaga gctagatcag
    30181 acttttgcta aattcgcaaa agactttgca cattttgcta atttcacagt accgctctgt
    30241 gacgtactat agtcgacaaa cgtctcacct tgagattaaa aaatgagcaa catcctgatt
    30301 atcaacggcg cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac cctgaccgaa
    30361 gtcgcggatg gcacactgcg cgacctcggg catgatgtcc gcatcgttcg cgccgacagc
    30421 gactacaatg tcaaagcgga agtacaaaac tttctctggg ctgatgtggt gatctggcag
    30481 atgccaggct ggtggatggg cgcgccgtgg acagtgaaaa aatatattga tgatgtattc
    30541 accgaaggtc acgggactct gtatgccagc gatggtcgta cccgcaaaga tccgtcgaaa
    30601 aaatacggtt ccggcagcct ggtacagggc aaaaaatata tgctttctct aacctggaac
    30661 gcaccaatgg aagccttcac cgaaaaagat cagttcttcc acggcgttgg cgttgacggt
    30721 gtgtatctgc cgttccataa agcaaaccaa ttcctcggta tggaaccgct gccgacattt
    30781 atcgctaatg acgtgataaa aatgcctgat gttccccgct atactgaaga atatcgcaag
    30841 catcttgtgg aaatttttgg ttaactagag ctcaggcttt agaaggagtt aaccatgctt
    30901 accgtaatcg cagaaatccg tactcgtcct ggtcaacatc accgtcaggc ggtattggat
    30961 cagtttgcta aaatcgttcc aaccgtactg aaagaagaag gttgccacgg ctatgcgcca
    31021 atggtggatt gcgctgctgg cgtgagtttc cagtctatgg caccggattc tatcgtgatg
    31081 attgagcagt gggaaagcat cgcgcacctt gaagcgcatc tgcaaacccc gcacatgaag
    31141 gcgtacagcg aagccgtaaa aggcgacgtg ctggagatga atatccgtat tctgcagcca
    31201 gggatttaat cctgccttgt ttgcccggcc atcctgaccg ggcaatgttc tttcctttaa
    31261 acctcaatct ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc ttccgagcgt
    31321 ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc atcatcgata
    31381 gtcaactgca ccagacggcg agtgttcgga tcaagcgtgg tttcacgcaa ttgcatcggg
    31441 ttcatttccc ccagaccttt aaaacgctgg acgttcggct tgcctttctt gcgttttaat
    31501 tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc tttcccgaga
    31561 tcaatacggt agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac caacgcgcgg
    31621 aagtgtttta cgaacaaagc gcagagcagc gtggcaatgt gcagaccatc agagtccgca
    31681 tccgcgagga tacagatttt gccataacga agctggctca gatcgtcgct gtcaggatcg
    31741 ataccgatcg ctaccgaaat atcatgcact tcctgcgagg ccagcacttc gtcggaagaa
    31801 acttcccagg tgttaaggat cttacctttc agcggcatga tcgcctgata ttcacgatcg
    31861 cgcgcctgct tggcagatcc gcctgcggag tcaccttcca ctaggaacag ttcggtacgg
    31921 ttaaggccct gcgcggtaca atcagccagt ttgccaggca gcgccgggcc gctggtcagc
    31981 tttttacgca ccactttttt agccgcacgc atacgacgct gggcgctgga aatcgccatc
    32041 tctgccagca actccgccgc ctgaacgttc tggttcagcc acaggatgaa ggcatctttc
    32101 accacgcccg aaacgaatgc cgcacactga cgcgaagaga gacgctcttt ggtctgcccg
    32161 gcaaactgcg gatcctgcat tttaaccgac agaacatagg cgcagcgatc ccagatatct
    32221 tccgccgaca gcttcacgcc gcgcggcaga atgttgcggt attcacagaa ctcacgcatc
    32281 gcgtccaaca ggccctgacg cagaccatta acatgggtac cgccctgcat cgttgggata
    32341 aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag tagcgcccag
    32401 tccacagctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg cagcgtcggt
    32461 aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca ccagcgctgt
    32521 tcggtgttgt tgatctcatc tttaaaggta atctcaacgc cggggcacag tactgctttg
    32581 gccttcagca catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa gaaggtttca
    32641 tccggccaga agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt
    32701 aaatcctgca ccttttcgcc attttcaaag gcgatgttat aaacctgacc atcgcggcgc
    32761 acgttaactt ctacgcgctt cgacaaggcg ttaactaccg aaatccccac gccatgcagg
    32821 ccgccagaga actggtaatt tttgttagag aatttaccgc ctgcatgcag acggcaaaga
    32881 atcagttcaa ccgccggtac tccttcttcc gggtgaatat ccaccggcat cccgcgccca
    32941 tcgtcaatca cttctaacga ctggtcagca tgtaagatta catccacgcg ttttgcgtga
    33001 cccgccagtg cttcatcgac actgttatca ataacttctt gccccaaatg gttagggcga
    33061 gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt gagtacctca
    33121 atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca
    33181 gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga agcccgtgaa
    33241 tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcgtagtacg ccaccgcctg
    33301 gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat ccggcgcttc
    33361 cagcgggtca atctgcatga ctttgagatc gtaaatatgg cgtgactcta gcacatattg
    33421 ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcaatt caaacgggcg
    33481 taccgccggg tttaccacca ctgcgggcag cataaaacat tgcgacaacc aggtggcgta
    33541 atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat
    33601 ggattccagc agttctgccg cgtcggaagg atacggcggc aactgcggaa tgatcatctc
    33661 aacgtcaggg tgatgttccg ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg
    33721 cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc tgaagcggta
    33781 tcaggttgga aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt gccatcagca
    33841 tgtaactcga gagtacgcca gccgggcgcg atagtatcca gcgtaaagtt ggaacagtgc
    33901 ggcttaaact gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt ccagtcgaga
    33961 tccagctcct gatgaatatg accgcacagc aagtatttga cgtgcggaaa cttcgccagc
    34021 acggtatcca gttcgcccgc gttacgcaga ctgtgttgat cgagccaact acaacccgca
    34081 ggtagcggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc ggccagttta
    34141 cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc aaacacctgg
    34201 ctatccagca acaggatttg ccattgctca ccaataaaca cccgcttcgc cggggagata
    34261 cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc cggcagccag
    34321 acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggc cgcagaggat
    34381 tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg gtgtggacga
    34441 atcgcctcca gcaccgcctg gtagctttcc caggtgttta cccctaacag ggcttcgtgc
    34501 ttttgtgcaa acaggtgagt atcggtaatt tgtaaaatcc ttcctctggc ctcaccagcc
    34561 agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg ctagtaaacc
    34621 ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa aaactggtta
    34681 atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata ccgcgctttg
    34741 aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg atacagacgc
    34801 accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc tattgtcact
    34861 agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt tgccacctga
    34921 tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag
    34981 ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt catttatgcc
    35041 cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat gaccgacgct
    35101 gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct taccacatga
    35161 acgcgaatat cttcgttttc atcagccaga ccatgaatac cgcttgcggt cgtggcgtcc
    35221 acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc caggaaactt
    35281 aacaccggtt tggtccgttt gactatcagt cccgcctctt caatcgcttc gcgacgggca
    35341 acatcttcca cactttcacc ctcttcaatc atcccggcaa ccatctccag tagccagggg
    35401 gtttcgctgg tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac ttcatcacgc
    35461 actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc
    35521 tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc taatgaaaaa
    35581 aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc aaatgtaacg
    35641 ggcaggttgt ctggcttaag cattgttaat gtcctagcac taatagtgaa ttaaatgtga
    35701 atttcagcga cgtttgactg ccgtttgagc agtcatatgt taaattgagg cacattaacg
    35761 ccctatggca cgtaacgcca accttttgcg gtagcggctt ctgctagaat ccgcaataat
    35821 tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg aagaaattgc
    35881 tccccattct tatcggcctg agcctttctg ggttcagttc gttgagccag gccgagaacc
    35941 tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag tctgccgccg
    36001 atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg ccacagctag
    36061 gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc atcaactcga
    36121 acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg aaatggcgtg
    36181 cgttaacgct gcaggaaaaa gcagcaggga ttcaggacgt cacatatcag accgatcagc
    36241 aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgct attgacgttc
    36301 tttcctatac acaggcacaa aaagaagcga tctaccgtca attagatcaa accacccaac
    36361 gttttaacgt gggcctggta gcgatcaccg acgtgcagaa cgcccgcgcg cagtacgata
    36421 ccgtgctggc gaacgaagtg accgcacgta ataaccttga taacgcggta gagcagctgc
    36481 gccagatcac cggtaactac tatccggaac tggcggcgct gaatgtcgaa aactttaaaa
    36541 ccgacaaacc acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc aacctgtcgc
    36601 tgttacaggc acgcttgagc caggacctgg agcgcgagca aattcgccag gcgcaggatg
    36661 gtcacttacc gactctggat ttaacggctt ctaccgggat ttctgacacc tcttatagcg
    36721 gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga tagcaatatg ggccagaaca
    36781 aagttggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac tcgcaggtga
    36841 aacaggcaca gtacaacttt gttggtgcca gtgagcaact ggaaagcgca catcgtagcg
    36901 tcgtgcaaac cgtacgttcc tccttcaaca acattaatgc ttctatcagt agtattaacg
    36961 cctacaaaca agccgtagtt tccgcgcaaa gctcattaga cgcgatggaa gcgggctact
    37021 cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgctg tacaacgcca
    37081 agcaagagct ggcgaatgcg cgttataact acctgattaa tcagctgaat attaagtcag
    37141 ccctgggtac gttgaacgag caggatttgc tggcactgaa caatgcgctg agcaaaccgg
    37201 tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct attgctgatg
    37261 gttatgcgcc tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc actaccacca
    37321 gtaacggtca taaccctttc cgtaactgat gacgacgacg gggcttcggc cccgtctgaa
    37381 cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct cgcttcaatt
    37441 tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag acaaaaatga
    37501 aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga
    37561 caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa aagagtgatg
    37621 aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg
    37681 ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat
    37741 acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgtcag caagcaccag
    37801 cccaggctgg catggcacca gaaaaccagg cgcaggctca gcaatccagc gggagtttct
    37861 ggatgccgct gatggccggt tacatgatgg ggcgtctgat gggcggcggc gcgggatttg
    37921 cacagcagcc gctgttctcc tcgaaaaacc cggccagtcc ggcttacggt aaatataccg
    37981 acgcgacggg taaaaactat ggcgcagccc agccaggccg caccatgacc gtaccgaaga
    38041 cggcaatggc accaaaaccg gcgaccacca ctaccgttac ccgtggcggt tttggtgaat
    38101 ctgttgccaa acaaagcact atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg
    38161 gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc gtgagaaagc
    38221 ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt gtgaagatgc
    38281 ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg ccgaactgca
    38341 ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga tgaccaaatt
    38401 ccgcatacca aaacacacct ggagttttgt gcgtcaatca tggctgactc accagccatc
    38461 gctttattcg cgtcttgatc tggcgtggga tggcactggc gaacctaaac ttctggaaaa
    38521 taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga tctggctgga
    38581 agatcagctt aacgccggta acttgtcgga gggcagcgac cagtttaaca gtctgcaaga
    38641 aaaactgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt tgctgcatct
    38701 cacctgctgt cgcgacacgg tggaagatcg cggaaccatt cagtatttgc aggactgcgc
    38761 aaccgaagct gaaatcgcca ccgagttcct ctacatcgac gagataggtt taggcgaaaa
    38821 aggccagttc acggatttac aggatcaggt gatttctaac ctgttcaagc tttatccgtg
    38881 ggaatttatg ctgcgcgaaa tgttctccac caagctggaa gatgctggcg tacgctggct
    38941 ggaaccggcg tggaagagca ttatctccaa caaggcactt ctaccgctac tgtgggagat
    39001 gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc atccgcaaat
    39061 ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga
    39121 gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga tgattgttca
    39181 gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta gctggctggt
    39241 gaacgatcaa cctgccggaa ttggcattcg tgaagaccgt gcgttgatca cccaggatat
    39301 gtctcggttt tatccacata tttttgttga ataagccacg ataccggatg gcactcgcca
    39361 tccggtaatt gttagcctat ctgcaccgac agcatactta aactgcccat ttctatacca
    39421 tcgaccggaa tggtaattgg ctcctgccca tcccacacac ctaacacata caacaacggc
    39481 agatagtgct ctggcgttgg gttcgataac gcgccacctt catggtcgag ataattcacc
    39541 agaggatgct gctctaccgg cccttgccac gtcagattcg ctttcacata ctcattaaac
    39601 gacgtcgccc acggatacgg tgaactatca ccgtgccact tcactgtgcg caggttatgc
    39661 accacgttac cgctggcgac caacattatt ccttcatcac gcagcgccgc gagtttgcgc
    39721 cccatttcga aatgccaggc gacaggtttg ctactgtcga tactcaactg caccataggg
    39781 atatcggcgt caggatacat cttaatcagc acgccccacg agccgtggtc aaagccccag
    39841 gcttctttat ccagcgtcac cgggatcggc gctaacagct caaccagacg ctgtgccagc
    39901 gcaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag
    39961 tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt aaaccagtga
    40021 gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt ctgccagctg
    40081 cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa aaacaatgct
    40141 ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt gatatcctca
    40201 caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc atcagttatt
    40261 ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt acctctcatt
    40321 ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt tctcggacaa
    40381 aaaccctcga accgcttact ggcgttttcg ctaggttttg cggcggggat catgttgctc
    40441 atctcattaa tggaaatgct tcctgccgca ctagcagctg aaggaatgtc gccggtgttg
    40501 ggttatggaa tgtttatctt cggtctgctt ggctactttg gcctggaccg catgttgccg
    40561 catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc tgttgccaaa atcgatcaag
    40621 cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga agggattgcc
    40681 acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcact ggccgtcgcg
    40741 ttgcacaata tccctgaagg tctggcagtg gcaggcccgg tttatgcggc aacggggtct
    40801 aaacgcaccg cgattctgtg ggcggggatt tctggactag cagaaattct tggtggtgtg
    40861 ctggcgtggt taatcctcgg tagcatgatt tccccggtgg tcatggcggc aatcatggcg
    40921 gcggttgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct cgccaaagag
    40981 attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt gatgggattc
    41041 agtttagtgc tgctacaaac ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc
    41101 acatggacaa cttctgccag cttcacgaaa gccttattct accttttcgg ctgtctcttc
    41161 tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa
    41221 cgcccgacaa taacgtttca aatgcaggaa ataacccacc acaaccagag caatcaatac
    41281 aatccagtac caggcgataa acatcctttt acccttaata tttaacaata ctaaacattt
    41341 agcgcataaa tttcacatat cctttttcgg atatattcat atggtcgcat aataacggaa
    41401 caattatatg attttgatca taatgaaaaa gaatgcctga tattaatgaa attttctcat
    41461 agaaagagaa gcggaattaa catcttgcat agcagggcaa aataaaaacc gaagccagct
    41521 acaacttcgg tttcttgatt aaggcagtaa ttaagcagcg gttttcagct ggctttacgc
    41581 tcatgtgcct gacggtatgc caccaggtct tcaatagtca cgagcgccat attgtgttta
    41641 ttggcaaact caatgcactc tggtgcacgc gccatcgtgc catcgtcatt agtcagctca
    41701 cacagcacac cagccggttt aaagcctgcc agcgtcatca aatcaatagt cgcttcagta
    41761 tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac gtggcccggg
    41821 cgattcagat cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacga
    41881 tcagcggcag aaacaccggt agtcacacct tcagccgctt caatggtcac ggtaaaaccg
    41941 gtgccatagg cgctggtgtt attttctacc atcattggca gatcaagttg tttacggcga
    42001 tcttcagtga tgcacaggca aacaataccg ctaccgtggc gaatggtcag cgccatctgc
    42061 tcaacggtca tggtttctgc cgggaagatc atatcgcctt cgttttcacg gtcttcatca
    42121 tcaagcacca ttacaccgcg tccttcacgc agcgcagcca gtgcattttc aacacgttcg
    42181 aaaggcgtac caaaagagga aagtagcgtc tgattcatgg taaaaaaacc tcactaaaat
    42241 tatggttacc agaatcaggg cagtcttagg agtggcggca tatagccaaa ataacgtgag
    42301 cgggtccatg cccgacagaa tcgttactct ctcccatccg gactctaacc gtcggccccg
    42361 gaattacacc ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg ggctttcaac
    42421 tgagttgatt taccgccggt ggggaatttc gccccgccct gagaataagc ggattcacta
    42481 taacgctaat gattagcggc agcaacgcat agcttcacat aattctggtt tatgacttac
    42541 ccttatcgca ctacaatggt actcaacacc tatcagtaca gggaaactac aatgattgac
    42601 ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat caatgcctaa aggaatcagg
    42661 gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc tacaagctca gctgacgcgc
    42721 ctcgaccttg taagccgtga agagttcgac gtccaaacgc aagtcctgtt acggactcgt
    42781 gagaaactgg cgctgcttga acaaagaatc agcgaactgg aaaatcgcag tactgagatc
    42841 aaaaagcagc cagatccaga gactctcccc ccaacgctgt aaacatttgt ctttaatgca
    42901 ctgtaaatta tgaattaatc ctctaattta cagtgttttc tattacatta tcgcttaatc
    42961 ccattcagag tatcatcaca aaaatgaatg attgcaattg aaaatataat tgttatgtat
    43021 tctttaagtt ttacttaatc gatatttaga ctggccc
//
LOCUS       DBKELA010000023        40704 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.023, whole genome shotgun sequence.
ACCESSION   DBKELA010000023 DBKELA010000000
VERSION     DBKELA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.023"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            145..486
                     /locus_tag="ACUQR0_001229"
     CDS             145..486
                     /locus_tag="ACUQR0_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HHV9108355.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(477..1403)
                     /locus_tag="ACUQR0_001230"
     CDS             complement(477..1403)
                     /locus_tag="ACUQR0_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9108356.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1493..2491
                     /locus_tag="ACUQR0_001231"
     CDS             1493..2491
                     /locus_tag="ACUQR0_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HHV9108357.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2488..2706)
                     /locus_tag="ACUQR0_001232"
     CDS             complement(2488..2706)
                     /locus_tag="ACUQR0_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HHV9108358.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2708..4723)
                     /gene="ligA"
                     /locus_tag="ACUQR0_001233"
     CDS             complement(2708..4723)
                     /gene="ligA"
                     /locus_tag="ACUQR0_001233"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HHV9108359.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4794..5780)
                     /gene="zipA"
                     /locus_tag="ACUQR0_001234"
     CDS             complement(4794..5780)
                     /gene="zipA"
                     /locus_tag="ACUQR0_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HHV9108360.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6010..6771
                     /gene="cysZ"
                     /locus_tag="ACUQR0_001235"
     CDS             6010..6771
                     /gene="cysZ"
                     /locus_tag="ACUQR0_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HHV9108361.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6956..7927
                     /gene="cysK"
                     /locus_tag="ACUQR0_001236"
     CDS             6956..7927
                     /gene="cysK"
                     /locus_tag="ACUQR0_001236"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HHV9108362.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8311..8568
                     /gene="ptsH"
                     /locus_tag="ACUQR0_001237"
     CDS             8311..8568
                     /gene="ptsH"
                     /locus_tag="ACUQR0_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HHV9108363.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8613..10340
                     /gene="ptsI"
                     /locus_tag="ACUQR0_001238"
     CDS             8613..10340
                     /gene="ptsI"
                     /locus_tag="ACUQR0_001238"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HHV9108364.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10381..10890
                     /gene="crr"
                     /locus_tag="ACUQR0_001239"
     CDS             10381..10890
                     /gene="crr"
                     /locus_tag="ACUQR0_001239"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HHV9108365.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10932..11783)
                     /gene="pdxK"
                     /locus_tag="ACUQR0_001240"
                     /gene_synonym="thiJ"
     CDS             complement(10932..11783)
                     /gene="pdxK"
                     /locus_tag="ACUQR0_001240"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HHV9108366.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11888..12262
                     /locus_tag="ACUQR0_001241"
     CDS             11888..12262
                     /locus_tag="ACUQR0_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HHV9108367.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12295..13018
                     /locus_tag="ACUQR0_001242"
                     /pseudo
     CDS             12295..13018
                     /locus_tag="ACUQR0_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13222..14133)
                     /gene="cysM"
                     /locus_tag="ACUQR0_001243"
     CDS             complement(13222..14133)
                     /gene="cysM"
                     /locus_tag="ACUQR0_001243"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HHV9108368.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14267..15364)
                     /gene="cysA"
                     /locus_tag="ACUQR0_001244"
     CDS             complement(14267..15364)
                     /gene="cysA"
                     /locus_tag="ACUQR0_001244"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HHV9108369.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15354..16229)
                     /gene="cysW"
                     /locus_tag="ACUQR0_001245"
     CDS             complement(15354..16229)
                     /gene="cysW"
                     /locus_tag="ACUQR0_001245"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HHV9108370.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16229..17062)
                     /gene="cysT"
                     /locus_tag="ACUQR0_001246"
     CDS             complement(16229..17062)
                     /gene="cysT"
                     /locus_tag="ACUQR0_001246"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HHV9108371.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDS
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17062..18078)
                     /gene="cysP"
                     /locus_tag="ACUQR0_001247"
     CDS             complement(17062..18078)
                     /gene="cysP"
                     /locus_tag="ACUQR0_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HHV9108372.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18382..19173)
                     /gene="ucpA"
                     /locus_tag="ACUQR0_001248"
     CDS             complement(18382..19173)
                     /gene="ucpA"
                     /locus_tag="ACUQR0_001248"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HHV9108373.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19302..20160)
                     /gene="murR"
                     /locus_tag="ACUQR0_001249"
                     /pseudo
     CDS             complement(19302..20160)
                     /gene="murR"
                     /locus_tag="ACUQR0_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            20324..21220
                     /gene="murQ"
                     /locus_tag="ACUQR0_001250"
     CDS             20324..21220
                     /gene="murQ"
                     /locus_tag="ACUQR0_001250"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HHV9108374.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21224..22648
                     /gene="murP"
                     /locus_tag="ACUQR0_001251"
     CDS             21224..22648
                     /gene="murP"
                     /locus_tag="ACUQR0_001251"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HHV9108375.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            22653..22739
                     /locus_tag="ACUQR0_001252"
                     /pseudo
     CDS             22653..22739
                     /locus_tag="ACUQR0_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22828..23727)
                     /gene="yfeX"
                     /locus_tag="ACUQR0_001253"
     CDS             complement(22828..23727)
                     /gene="yfeX"
                     /locus_tag="ACUQR0_001253"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HHV9108376.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23823..24398)
                     /locus_tag="ACUQR0_001254"
     CDS             complement(23823..24398)
                     /locus_tag="ACUQR0_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HHV9108377.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24459..24908)
                     /locus_tag="ACUQR0_001255"
     CDS             complement(24459..24908)
                     /locus_tag="ACUQR0_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HHV9108378.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(24895..25320)
                     /locus_tag="ACUQR0_001256"
     CDS             complement(24895..25320)
                     /locus_tag="ACUQR0_001256"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HHV9108379.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25534..26403
                     /gene="amiA"
                     /locus_tag="ACUQR0_001257"
     CDS             25534..26403
                     /gene="amiA"
                     /locus_tag="ACUQR0_001257"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HHV9108380.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26407..27306
                     /gene="hemF"
                     /locus_tag="ACUQR0_001258"
     CDS             26407..27306
                     /gene="hemF"
                     /locus_tag="ACUQR0_001258"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HHV9108381.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27312..28364)
                     /gene="eutR"
                     /locus_tag="ACUQR0_001259"
                     /pseudo
     CDS             complement(27312..28364)
                     /gene="eutR"
                     /locus_tag="ACUQR0_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28410..28910)
                     /gene="eutK"
                     /locus_tag="ACUQR0_001260"
     CDS             complement(28410..28910)
                     /gene="eutK"
                     /locus_tag="ACUQR0_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HHV9108382.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(28923..29582)
                     /gene="eutL"
                     /locus_tag="ACUQR0_001261"
     CDS             complement(28923..29582)
                     /gene="eutL"
                     /locus_tag="ACUQR0_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HHV9108383.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29592..29660)
                     /locus_tag="ACUQR0_001262"
                     /pseudo
     CDS             complement(29592..29660)
                     /locus_tag="ACUQR0_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30764..31555
                     /locus_tag="ACUQR0_001263"
     CDS             30764..31555
                     /locus_tag="ACUQR0_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108384.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32275..32670
                     /locus_tag="ACUQR0_001264"
     CDS             32275..32670
                     /locus_tag="ACUQR0_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHV9108385.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32688..32951
                     /locus_tag="ACUQR0_001265"
     CDS             32688..32951
                     /locus_tag="ACUQR0_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108386.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            32974..33516
                     /locus_tag="ACUQR0_001266"
     CDS             32974..33516
                     /locus_tag="ACUQR0_001266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108387.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33655..33942
                     /locus_tag="ACUQR0_001267"
     CDS             33655..33942
                     /locus_tag="ACUQR0_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108388.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            33981..34187
                     /locus_tag="ACUQR0_001268"
     CDS             33981..34187
                     /locus_tag="ACUQR0_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108389.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34499..35141
                     /locus_tag="ACUQR0_001269"
                     /pseudo
     CDS             34499..35141
                     /locus_tag="ACUQR0_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            35236..36696
                     /gene="gadC"
                     /locus_tag="ACUQR0_001270"
     CDS             35236..36696
                     /gene="gadC"
                     /locus_tag="ACUQR0_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HHV9108390.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36730..37539
                     /gene="murI"
                     /locus_tag="ACUQR0_001271"
     CDS             36730..37539
                     /gene="murI"
                     /locus_tag="ACUQR0_001271"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HHV9108391.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37658..38352)
                     /locus_tag="ACUQR0_001272"
     CDS             complement(join(37658..38106,38106..38352))
                     /locus_tag="ACUQR0_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HHV9108392.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(38871..40244)
                     /gene="eutA"
                     /locus_tag="ACUQR0_001273"
     CDS             complement(38871..40244)
                     /gene="eutA"
                     /locus_tag="ACUQR0_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HHV9108393.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40219..40698)
                     /gene="eutP"
                     /locus_tag="ACUQR0_001274"
     CDS             complement(40219..40698)
                     /gene="eutP"
                     /locus_tag="ACUQR0_001274"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HHV9108394.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        10297 a        10480 c         9871 g        10056 t
ORIGIN      
        1 gcgagtcatc ctgcacgacc caccaatgca aaaaagcgcc ctaaaggcgc ttttttgcta
       61 tctgcgattt gtaagattga tttttcttct caagttcctt gttcttctac cgatattacc
      121 tcgtacagtg acgaggactg aacaatggcc actatctctt caacttcaat tccttccata
      181 caaacacaga gtagtaacag ggcatcacaa ggtagcgatg ttgcttcaca aatcgcgcgg
      241 ataagtcagc aaatcatcaa actcactcaa caaattaaag agattgtaga cactagcggc
      301 agtgctgaag acaagcaaaa acaggccgaa ttgattcaac agcaaatcac cctactggaa
      361 acacaactgg cgcagttgca gaaacagcag gcagaaaagg cgcaggagaa agagcagcgt
      421 ttgtctctga atgtatccct tctaaatcct gttgaaaaaa cgactcatat cgatatctat
      481 atctgataca acggatcccc ttcccgacca gccattagcg cattgccctg gtctcgtacc
      541 acgtcccata acgcttcagc ggcggtggaa agtgaacggt tcttcctgcg caccagcatc
      601 aactggcgtt caaccaccgg cgtaatgcgc ttaaccacca gtgggctacc ttcaggcaat
      661 ggtagcgcca gtgcaggaag aatactgatg ccgatgcctg cggctaccat cgggaacagc
      721 gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa taccattgcg cgccagcgct
      781 gcgtcaatca gcggtcggct gcctgacgcg taatcctgca acaccaattt cgctccctgt
      841 aatgcctgcc agggtacata atcttccacg gccaaagcgc tatcgcgatg gcaaagcaga
      901 aagaaaggct cggaaagaat cgcttcacat tgcagatcgc ccactgggcc tggatcgatg
      961 acgatgccaa agtcgacatc tccctggcga atactttcca tcacccactg ctgcggacga
     1021 tcatgcagga caaactgaat atctggatag cggcgatggc tttcagcaat gcattgcgga
     1081 ataagatgcg cggaaatggt ctggctggca gcgacccgaa cttttccgct cagttgttgc
     1141 cccatacgcc cggtatcgcg caacgtgctg ttaagttcat ccagcagtcg ctcaagacgc
     1201 aaagccaact gctgccctgc atctgtaagc accacttcac gcgtggttct gtccagcagg
     1261 cgaacaccag tatgattttc cagctccttc acactgtgac ttactgccga ctggctcaag
     1321 ccgatacgct ctcctgcacg actaaaactt ttctcctgcg ctactgtgac gaaaaccttt
     1381 aattgtttta aagaataatt catctgtttt cttcatgaat ggatgcaata aatcaatttt
     1441 atttctcaaa ctggaagaag cacaatagaa ccatcgatca tctggagtct ttatgaaact
     1501 ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg gtggtgttgc tggcctcttt
     1561 ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat ctgaccaccg cagctattgc
     1621 cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag gcgattattg ctggcggtgg
     1681 tcactggcga ctgcatttgt gggtaatgtg cagcaccttc gtgctgtttc cgattctggg
     1741 tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg atgctctact ccggttttct
     1801 ctacttgtgc attctcccgg ctaccgtgca gtctgcaatc gccttcacgt caatggcggg
     1861 cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc agcctgctgg ggattttcct
     1921 ttcaccattg ctggttggtc tggtgatgaa tgttcacggt gcaaggggca gccttgagca
     1981 ggtcggtaaa attatgctgc aactgctgct gccgtttgtg ttggggcatc tttcccggcc
     2041 gtggattggt gactgggtgt cgcgcaataa aaaatggatt gcgaaaactg accagacgtc
     2101 cattctgttg gtggtttata cagcgttcag cgaagccgtc gttaatggta tctggcataa
     2161 agttggctgg ggatcattgc tgtttatcgt ggtggtcagc tgcgttcttc tggctatcgt
     2221 gattgtagtt aacgtcttta tggcacgccg actgagcttc aataaggcag atgaaattac
     2281 tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc atcccgatgg caaacattct
     2341 gttccccaca tcggtgatcg gtatgatggt gctgcccctg atgattttcc atcagatcca
     2401 attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc cagaccgaac agttacaggc
     2461 gcagcaggaa agcagcgccg ataaagctta aagcggacgc ttcaggggct ggaccagttg
     2521 cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt agctcaccga gcttccctgc
     2581 cgggaactca tctttgcggg caaaccacag cagatactcc tccggcaggt caattaagcg
     2641 acgccctttg tatttgccaa acggcattat cgtattggct atttcaatca gctgctcttt
     2701 ttccatctca gctacccagc aaacgcagca tttccgcttc gtcgatgact tcaatgccca
     2761 gttcctgcgc cttcgccagt ttagatcctg cagcttcacc cgctatcacc agatcggttt
     2821 tcttcgacac gctgcccgcg actttcgcgc ccagttcgac cagtcgagct ttagcgtcat
     2881 cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt tttaccagca aacgggctgt
     2941 caatctcttc cgcgttgata acgatcggcg caggccagtg aacaccttcc gccaacagct
     3001 cgctgatgac attgcggttg ctttcttcgg caaagaagtt gtgaacgtgg gatgcaacga
     3061 caatgccaac atcaggcacc ttttgcagct cttcaatcga agcggcttcc agcgcttcca
     3121 gcgtgccgaa atatgccgcc agacctgctg cggtggcctc gccgacttca cggatgccaa
     3181 gtgcatagag gaagcgagca aaggtggttt ctttcgcttt ttccagcgcg ttaaccacgt
     3241 tttgtgccaa ttttggcccc atacgctcca gtccggtcag tttgcctgcg gtgagtttga
     3301 acagatctgc cggagtatgg acatattctt tttcaaccag ctgatcgatg attttgtcgc
     3361 ccattccgtc aacatccatc gcacggcggg aaacaaagtg tttcagcgac tctttacgct
     3421 gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc ttcaccttcc acacgctcaa
     3481 cgtcagaacc acataccgga caatgcgtcg ggaatacaac ctcacgggta tcttccgggc
     3541 gttcagaaag cacgacgtta accacctgcg ggatcacgtc gccagcgcgg cgaatcacca
     3601 ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc cgcattgtgt aaggttgcgt
     3661 tactcaccag cacgccagca acatggacag gttccagacg cgcaacaggc gtaatcgcgc
     3721 cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt catctgctcc tgcgccggga
     3781 atttaaacgc aaccgcccag cgcggggcac gcgcgacaaa gccaagctgc tcctgctgtt
     3841 ccagtgagtt gaccttaatc accacgccgt cgatatcaaa gcccagcgtc gggcggtctt
     3901 cttccacttt gtgatagaac gccagcactt cttccgccga ttcacaaagc gttacccgat
     3961 cgctgaccgg caccccccac tttttaaatt gcagtaaacg gccaagatga gtatccggca
     4021 gctcgccacc ttccagaaca ccaacgccat agcagaaaaa agtgagcggt cgcttcgctg
     4081 taatacgcgg atcaagctga cgcagtgaac cagctgccgc attacgtggg ttagcaaaaa
     4141 ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc gaaccccgcc tgcggcagga
     4201 acacttcacc acgcacttcc agacgcgccg ggatattctc tccgtgcagc ttcagcggaa
     4261 tggcgcgaat agtacgcaca ttagacgtga tatcttcccc ggtggtgcca tcgccacggg
     4321 tcgcggcact gactaaaacg ccattttcat acagaatact gacggcaagg ccatccagct
     4381 tcagctcaca gcaccaggtg actttctcgt tgtttttcag acggtcctgc acacgtttgt
     4441 tgaaagcaag aaagctttct tcatcaaaaa cgttatccag tgacagcatt ggtacttcat
     4501 ggcgtatctg gctgaaagct gccagcggcg cagcacctac acgttgggta ggcgaatcag
     4561 gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag ttcgcgcatc agcctgtcgt
     4621 attcagcgtc gggaatttcc ggcgcatcca tcacatgata aagatattca tgatggcaaa
     4681 gcgtcgttcg cagttctgtc agttgttgtt cgattgattc catatcgcac catcaatgct
     4741 aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt tgccttaagt gtatcaggcg
     4801 ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc gcaatttctg cggagtcatc
     4861 atacggcgct gatcgtcaag cacgacaccg cccacttcat cggcaatatg ctgcgcagat
     4921 tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag acggtacctg cataaagata
     4981 gtgacacccg gagtagtgaa atccttcatt tcaggatcaa aggttcccgg tttcaccata
     5041 ttcgccaggc tgaataacgc cgggccgctg ccatccgggc taagatgacg atggtaaata
     5101 ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt taagaagcag ttcaccgttt
     5161 agctcgctac cgtgatgcgc cgcgacgttc atgataatca ccgcttcttt gcgcttcggt
     5221 ttatccataa ctggagccgg ttccgctaca ggctcaggct gtggtgccgc tacgggttct
     5281 gcaggctgga aagcctgttg tgccggttgc ggtgctgaat gcacaggctg cggcgctggc
     5341 gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag gctgcggctg ataggcaggc
     5401 tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc
     5461 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gtaggtactg gtgttgcggc
     5521 gacggacgag cagcctcatg ctcctgagcg ttagccgggg catgattcac gcggtgaaca
     5581 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
     5641 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
     5701 gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
     5761 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
     5821 catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca
     5881 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
     5941 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
     6001 atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg
     6061 caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg
     6121 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct cgatgtctgg
     6181 atcccgactt tcatgagtta cgttccggac tggctacaat ggctgagtta tctgttgtgg
     6241 cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac gattgctaac
     6301 tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg attgactggc
     6361 gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg aatcatgaaa
     6421 cgcgaatggc aaaaatttgt ctggtatctg ccgcgcgcaa ttgtattact aattctttac
     6481 ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg
     6541 atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag
     6601 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
     6661 ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt ttgtggcgcg
     6721 acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta acaatctacc
     6781 ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
     6841 ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt ccgcatattc
     6901 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
     6961 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
     7021 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
     7081 gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
     7141 cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct atgtagctgc
     7201 cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg aacgccgcaa
     7261 gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag gcatgaaagg
     7321 cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
     7381 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggaaatctg
     7441 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
     7501 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
     7561 cgttgagcca accgattctc cagttatcgc ccaggtgctg gcaggtgaag agattaaacc
     7621 tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc tcgatctcaa
     7681 gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cccgtcgtct
     7741 aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg ccgcagcgtt
     7801 gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttattc taccatcatc
     7861 gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga aagaattgca
     7921 acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg
     7981 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
     8041 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
     8101 attttatgat ttggttcaat tcttccttta gcggcataat gcttaatgac gtacgaaacg
     8161 tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt cgcgtctgtt
     8221 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
     8281 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
     8341 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
     8401 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg
     8461 cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag
     8521 cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
     8581 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
     8641 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
     8701 aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt cgtgccaagg
     8761 catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag
     8821 aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca
     8881 tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa gttatcgaag
     8941 gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg
     9001 tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga
     9061 gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc gaaaccgcac
     9121 agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca
     9181 cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct
     9241 ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca
     9301 atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa
     9361 aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag
     9421 aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg
     9481 aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg ctgcccactg
     9541 aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg caggcggtta
     9601 tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag
     9661 aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc
     9721 tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg cgcattatgt
     9781 tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc gaaatctaca
     9841 aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg
     9901 tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta
     9961 gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga
    10021 tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg
    10081 atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac
    10141 gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta
    10201 tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag
    10261 agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg
    10321 aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta ggagaagatc
    10381 atgggtttgt tcgataaact gaaatctctg gtttccgacg acaaaaagga taccggaact
    10441 attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc
    10501 gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg taacaaaatg
    10561 gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc gttctctatc
    10621 gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa
    10681 ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc
    10741 attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt
    10801 atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt
    10861 gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc
    10921 ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct ctcatgctgc
    10981 tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcaccgc atcgtttaac
    11041 gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc agtccctttc
    11101 aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact gaccaccacc
    11161 acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac ccattttaat
    11221 gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg gcaattttta
    11281 ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag cggcagtaaa
    11341 tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc aatatcgcca
    11401 atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc agtcagccac
    11461 tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac agcacgaagt
    11521 tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca ttcgtccgga
    11581 atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac cgtcggcacg
    11641 gcaaagacat tcaagccgtt ctgttttata gcaggcaccg caatgctgtt gcctacgctt
    11701 ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagcgccct actcttatcg
    11761 ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc tcatcgtatt
    11821 ccaaccgaaa ctttacctga ttctggcagt caaatcggct attacaaaac aaagataagg
    11881 taattcagtg aagaaaatca tttgtctggc cattacgctg ctaatgacac tccccgctta
    11941 cgcgaagtta actgttcatg aagaagcccg catcaacgcc atgctggagg gattagcaca
    12001 gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat acctgcgatg aagcggtttc
    12061 ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg agcagtttat
    12121 tgataaggta gcttcgtcgt catcgattac cgggaagccg tatatcgtga agatccccgg
    12181 taagagcgat gagaacgcac agcctttttt acatgcgtta attgcgcaga cggataaaac
    12241 ggtgcctgcg gaaggaaatt aatccgcttt ggtaaggcat ttacaggagg taacatgaaa
    12301 aaatgcttta tttatcacga tgaaaaatcg aataaatttt ggtggataga ttacgaaggg
    12361 gatagtttag ctgtcaacta tggcaaggta ggtagtattg gtaaattcca tacaaaagaa
    12421 ttcgataatg aagaacagtg tctgaaagaa gccagtaaat tgattgccgc aaaaatgaaa
    12481 aaaggatatc aagaaactcc agactttaac ttcatggatc tctactattt tgatgatgaa
    12541 gagattgggt tacatcttaa aacgtcacac ccaaacttcc agtgccattt tactgatcca
    12601 ctttatatgt gttgctggga tgaagaatct ccttttggta gcgatgaagg tgctgatgct
    12661 ctaaacgaac ttgaaaatag cctccgttct ggactgtgct gatttccctc aaatgttaat
    12721 tgaaactatg tggggtatga aatacatcgc tatggacagt attcttgaag aggatgtgcg
    12781 tgcgcaatta ctagccgatg aaatgagctc tatccagagc aatatgatta cctacgcgac
    12841 tgcattcggt cagatcaaag tcatgggtaa aatctcccat aaacttaaaa agatgggact
    12901 caatgcacta gcgcgtcatc agcttaccgc aaaaattctt caatggggtg acggtcagga
    12961 ctcaccaata cttcaaaaaa tgattgatga ccttactgct tttccacacg aaaattaaaa
    13021 actgcatttg tcggcagcaa caactgccga caaatcagat tattgacaaa gtgcactttg
    13081 tttatgccgg atgcggcgta aacgcctgat ccggcataaa aatattgcta attcaatata
    13141 ttgcagaaac attgtaggcc tgataagcgg agcgcatcag gcagttttgc gtttgtcatc
    13201 agtctccaat gctattaatc cttaaatccc tgccccctga ctaaaatgct cttccccaaa
    13261 cactccggtg gagaggtagc gatcgccacg atcgcagatg atcgctacca ccaccgcacc
    13321 agggttagct tttgccaccc gcagtgctcc agcaaccgcg ccgcctgagc tgacgccaca
    13381 gaatattcct tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat
    13441 atccagcacc tcatccacca gcgcagcgtt gaaaatcccc ggcagatatt ccgcaggcca
    13501 gcggcgaatg cccggaatac tgctgccttc ttccggttgc aggccaacaa tggtcaccgg
    13561 tttggattgt tcgcgcataa agcatgaaac gccggtgata gtgccggtcg tccccatgct
    13621 ggagacaaaa tgagtgatgc gcccgccggt ttgctgccag atttccggcc cggtggtggt
    13681 gtaatgcgca taagggttat cgggattatt gaactgatcg agcagctttc cttcgccacg
    13741 attcgccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt tggtgacaag
    13801 aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca tgttgtcggg
    13861 catcagcaat ttcatgcgat agcctttcag cgcggcaatc attgccagcg caatgccggt
    13921 gttaccactg gtggcttcga ttaagacatc acccggttta atttccccgc gcttttccgc
    13981 ctcgacgatc atcgaaagtg ccgcacgatc tttcaccgaa cctgccgggt tattgccttc
    14041 cagttttaac cacacttcac tgccgttatc cggccccatt cgctgcaact tcaccagagg
    14101 cgtattgcct attgtttgtt ctaatgtact cacaatctct atccatacgt ggtgttgcct
    14161 gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc
    14221 cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg
    14281 cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac agccgcgcat
    14341 gttgcagacc aacgaataaa cgctcgccat gctgcggggc atcgtcgcca tgcatcacga
    14401 ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg tagtgacctt
    14461 tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gagactggta cggcggctga
    14521 tatccacttc ccacgggcgc aggaagagat ccaccggccc ctgatacgca ggtgtgtagc
    14581 ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc
    14641 ggttcacttc gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc catacctgat
    14701 tcggcgcatc agcctgttca atattcccct gactcatcac cactacacga tcggctactt
    14761 cggtcgcctc ttcctggtcg tgggtcacaa acacgctggt gaatttcagc tcttcatgca
    14821 gttgacgcag ccagcgacgc agctctttac gcacctgcgc atccagcgcg ccgaacggtt
    14881 catcaagcag cagaatttgc ggttctacag ccagcgcgcg cgccagcgcc acacgctgtt
    14941 tctggccgcc ggaaagctgc gccggataac gatccgccag atgcgcaagc tggaccattt
    15001 ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg cgacgcggca
    15061 gcaccgtcag gccaaaagtg atattgtcga ataccgtcat atggcggaac agcgcgtaat
    15121 gctggaacac gaaaccgact ttacgatcgc gcgcgtgcag gcgactcacg tcggtgccgt
    15181 ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatccgc agcagcgtgg
    15241 ttttcccgga accggacggc cccagcaacg cgaccatctg accagaagga atatccagtg
    15301 agatatcgtt cagcacctgg gtgcgaccaa aagacttctt gatattggca atctcaatgc
    15361 tcatgatgct cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca ctgcaacata
    15421 ctctttaaaa acagggtgat aatcgccatc aacgtcaaca gcgcggcggc ggtaaaggag
    15481 ccgacggtgt tgtagtcctg ctccaacaat tcaatctgta acggcagcga aagtgtttcg
    15541 ccgcgaatcg agccggaaac caccgacact gcaccaaact cgccaatcgc gcgggcgttg
    15601 gtcagcacca cgccgtaaag cagcgcccag cgaatattcg gcaatgtgac gcgacggaac
    15661 atctgccagc cggacgcgcc aagcaaaatc gccgcttcgt cttcctggct gccctggctt
    15721 aacatcaccg gcaccagctc gcgcaccaca aacggacacg tcacaaagat ggtgaccagc
    15781 accattccgg gccaggagaa cataatttgc agattatgtt catccagcca gccgccgagc
    15841 gggccgttgg agccgtagaa cagcagatac accagaccgg caaccaccgg cgatacagca
    15901 aacggaatgt ccagtagcgt cagcagtaac tggcgtcccg ggaagttaaa gcgcgtcacc
    15961 agccaggcca gcagaatgcc gaacaccagg tttaccggta cggcaatcag cgcgatcatc
    16021 accgtcagcc agatggcgtg cagcatgtcc ggatcggcca gattctgtaa aaccggcatc
    16081 agccccttgc tgaatgcctg cacgaagatg taaatcatcg gcaccagcag gatgaacgcc
    16141 gaaaccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca
    16201 taacgcttca attgggtaac ttccgccatt aatgacctac cacacgccga ccaaagcgac
    16261 tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc accgaagcaa
    16321 tcgcgctcgc tgccgggtaa tcaaactcct gtaagcgcac aaaaatcatc agcgacgtca
    16381 cttccgtctt ccacgcgata tttccggcga taaaaatcac cgcgccaaat tcaccaagac
    16441 tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc ggcagcacca
    16501 ctttgcagaa actctgccag cgcgttgcac caagcgtttc cgccgcttct tcatattccg
    16561 ggcctaactc ttccagcacc ggctgcacgg tacgcaccac aaacggaatg ctggtaaagg
    16621 ccatcgccac cgcaatacca agccaggtat aggtgacttt gatatcaaac ttcgccagcc
    16681 attcaccgta aaaaccgttt acggaaaaga gcgaggccag cgttaaaccg gcgactgccg
    16741 ttggcagcgc aaagggtgaa tccatcagcg catcaagcag cgtgcggccc ggaaagcgat
    16801 aacgtgttaa aatccacgcc atcagcagac cgaaaacgcc gttaaaaatc gatgccacaa
    16861 acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg atcacctccc
    16921 agtactgcgc ccagctcatc tcagaaagct gcatcaccag cgcagagagc ggcagcagca
    16981 atatcaggca cacaaacagc agactggtgc caaggcttaa agtaaagccc ggcagcacgc
    17041 gtcttgaaga aactgcaaac attacttacg ccccgccgct aacagcttgt ctaactcgcc
    17101 gccgctggtg aagtgggttt tcatcacttc cggccatgag ccaaatttgt cttccacgcg
    17161 gaacagttcg gtctgcggga atttatcttt cagtttgtcc atgacttccg ggttattcac
    17221 acggtagtaa tagtcggtga tgatggtctg cgcctgtggg ctgtagagcc agttcaggta
    17281 ggcttttgcg gctttttccg taccgttggc ctgcacattt ttatcgaccc acgccaccgg
    17341 gaattccgcc agaatattgg ttttcgggat caccacttca aagccctgtg cttcatactg
    17401 tttacggatg ttgttcactt ccgattcaaa gctaatcagc acatcgccca agccacgctc
    17461 ggcaaaagtg gtggttgcgc cacgaccgcc ggtatcgaac acttcaacgt ttttcaggaa
    17521 ctgggtcata aactgttcgg ttttggcttt atcgccaccg tcagctttat ccgctgcgcc
    17581 ccatgccgcc agataggtat aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag
    17641 cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt tgcccttacg
    17701 caccaggaag cccatggtgg agtagaacgg cgagctatta ttcggcaggc gcgactgcca
    17761 gtcagccggg atcagcttgc ctttgtcgtg caggatttgt acgtcggtca cctggttata
    17821 agtgacaacg tctgctttta agccctgcaa aatcgccagc gcctgttttg atgacccggc
    17881 atgagattgt tttatcgtca gtttgtcgcc gccgttatct tttgcccatt gctgttcaaa
    17941 cggcggattc agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc
    18001 cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg agttcttttt
    18061 cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat
    18121 atttataacg gacgtgaaag gagtaacggt tttatatacc gtttggtgat ttggaagtta
    18181 aaaagggaat aagaacttgg ttcaacaggc gtaattgttg caggcagttt ggacacggac
    18241 agcgcggaga aaccggagcg tacacatagt acgtgaggat ttcgagcact gcccggggcc
    18301 aaaatgacaa atgaaatagc ctggtgaact tagttcaaga cgaaatcctc cccacacatg
    18361 cagggaggaa acagaggtga atcagatacc gacgctaacc gtctccggca gtgtgctgcc
    18421 gccatcaatc acattctgtg taccggttaa atagctggat tcatccgatg cgaggaaggc
    18481 cgccagttcg ccgacttcca gcggatcggc gaggcgacac agcgggattg cttttgccat
    18541 ttcagtcagc accgattctg gatcttccgg gttcgactgg cgggcaatgc tttccgccat
    18601 tggcgtgcgg acgtatcccg ggcagatggc gttaacgcga ataccggatt gcgcgtactc
    18661 caccgccagc gatttagtca ggccaacaat cgccgctttc gttaaggcat acgccgtttc
    18721 gccaggatcg gccaccatat caccagtgac tgaagacatc atcacaatgc gaccatcttt
    18781 gcgcgcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt taatattgat
    18841 atcaatatgg aaatcgcgat cttcatcgct catatcgagg aaactgccca gacgacaaac
    18901 gcctgcgtta ttcaccagga tatcaatgcg cccttctttt tccttcgcgc gtttgatagc
    18961 tgcggctacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc gatgaccacg
    19021 accacacagt tcgtccgcta gcttttcgat ctcaggggag atatccagca agattaggtt
    19081 cgcgccatga cgtgcaaaag ttctggcaat tccttcgcca attccctgca atgcgcccgt
    19141 aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt taatacagag
    19201 cgttacactc cattccctct gaaaatacag gataaacaaa atattgctgc ggatcagctc
    19261 actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag gcgttgtgtt
    19321 agctcactgc tgcgctgaat cattttcaat gacgccacat cattgatgtt gcacaagacc
    19381 aacaaacagt aaatcggtaa cagaattttg cgccgtgcgg gtagacatcg acgagctacg
    19441 ccattcggtt tcaccggaaa cggtatcgag cgtgaaatgc gccagccgcc gcagcggaga
    19501 gtcagccaga gaagtaatgg caatcaccgt tgccccctgc tttcgtgccg cttctgcgca
    19561 caagacaatc tcttttttac tgccgctgta agagatagcg atttgtacat cgcctttttt
    19621 caacgcctgg gaaacggtgg cttgtacgtg cgtgtcggct tcgcaggcca cgcgatagcc
    19681 aattttcatc agtttgaacg acagatcgcg ccccaccagc gccgatccgc ccaggcccgt
    19741 tatctggata aacggtgctt tactgatgac ctcaatgatt ttttgcagcc gcgcgtagtc
    19801 gaacaacgca caggtttgct ccagcgccag ctctttttcg cgattcagtt tgcgggcaat
    19861 cacttccagc gaatcatcac tggtaatcga actgtgcagg tgcagcgccg tggcattcgt
    19921 tttctcccgg ctggcactgt attcgccaat taacgccata cgcaattcag taaatccttg
    19981 cgcgccgagc ttctgggcaa atttcacaat gctcgactgg ctgataccga gctgtttcgc
    20041 catctgccgt gaagagacgg atttcagctc gctcactctg gcccgcagaa agtcagcaat
    20101 tttttgctcg ttcccggtaa attcactttc cgcattgcgt attttcgtca ggtacaacat
    20161 cttccactct cctgggctgg cagcaaaaat tccgcttaag ttcttatgac gctcttcaca
    20221 ctctgcgagt gtagactcaa tgattccttt aagtctgtca attcgggata tatgattcca
    20281 tacatctgca attatttgat cgtaaatagt aaggtcacca ccgatgcaac tagaaaagat
    20341 gattactgaa ggctcgaaca ccgcctcggc tgaaattgac cgcgtatcga cgctggaaat
    20401 gtgccggatt atcaacgatg aagataaaac cgtaccgcct gccgttgatc gcgtcctgcc
    20461 ggatatcgcc gcggcgatcg atgttatcca cgcccaggtc agcggcggcg gtcgtctgat
    20521 ttatctcggt gcgggaacat ccggtcgtct ggggattctg gatgccagcg aatgtccgcc
    20581 cacctacggc gtgaaaccgg gtctggtggt tggtttgatt gctggaggcg aatatgccat
    20641 tcagcacgcg gtggaaggag ctgaagatag ccgggaaggt ggtgttaacg atctgaaaaa
    20701 tattaattta acggcacagg atgtggtggt tggcattgct gccagcggtc gcacgccgta
    20761 tgtgattgcc ggactggaat acgcacgcca gctcggctgc cgcacagtgg gaatttcctg
    20821 taatccgggg agcgccgttt caaccaccgc tgagtttgcc attacaccga ttgtaggtgc
    20881 cgaagttgtt accggttctt cgcggatgaa agcaggtaca gcgcagaaac tggtgctcaa
    20941 tatgctttcc accgggctga tgattaaatc cggcaaagtg ttcggcaacc tgatggtcga
    21001 tgtggtcgcc accaacgaaa aactgcatgt gcgccaggtc aatatcgtta aaaacgccac
    21061 cggatgtaat gccgaacaag cggaagcggc gttaattgcc tgcgagcgca actgtaaaac
    21121 ggccattgtg atggtgctga aaaatctcga tgccgcagaa gctaaaaaac gcctggatca
    21181 acacggcggc tttattcgtc aggttttaga caaggaataa cccatggcca aagagatcag
    21241 cagtgaactt ctgaacacca ttcttacccg tgtcggcgga ccgggaaata tcgccagttg
    21301 tggtaactgt atgacgcgcc tgcgtctggg tgtacatgac agttcactgg ttgatcccaa
    21361 tatcaaaacg ctggaaggcg tgaagggcgt cattttgacc agcgaccagg tgcaggtcgt
    21421 ttttgggcct ggtaaagcgc atcgtgccgc aaaagcgatg agcgagctgc tgggcgatgc
    21481 accggtacag gatgccgcag aaatcgccgc ccagaacaaa cgtcagttaa aagccaaaca
    21541 aacctccggc gtgcaacaat ttctcgccaa attcgccacc atcttcacgc cgctgatccc
    21601 cggttttatt gccgccggtc tgctgctggg gatagcgacg ttaattgcca cggtgatgca
    21661 cgttccggca gatgctcagg gcacgctacc cgacgcgctg aattttatga aggtgttcag
    21721 caaaggtttg ttcaccttcc tagtgattct ggtgggctat aacgccgccc aggcatttgg
    21781 cggcacgggc gtcaatggcg caattattgc cgcactcttt ttgctcggtt ataaccccgc
    21841 tgcaaccacc ggttactacg ccggttttca cgatttcttt ggtctgccca tcgatccgcg
    21901 cggcaatatt atcggcgtgc tgattgccgc gtgggcctgc gcccgcattg aaggcatggt
    21961 gcgccgcttt atgccggacg atctcgacat gctgctgacc tcgttaatca ccctgctgat
    22021 caccgctacg cttgcgtacc tgatcattat gccgctgggt ggctggttat tcgaaggtat
    22081 gtcatggctg tttatgcacc tgaacagtaa tccgttcggt tgtgcggttt tagccgggct
    22141 gttcctgatc gccgtggtat ttggcgtgca tcagggcttt attcctgttt acctcgcgtt
    22201 aatggacagc cagggattca acagcttatt tcccatcctt tcaatggcag gcgcgggcca
    22261 ggtgggcgcg gcgctggcac tctactggcg ggcgcaaccg cacagtgcgc tacgcagtca
    22321 ggtacgcggg gcgattattc ccggcctgct gggcgttggt gaaccgctga tttacggtgt
    22381 caccctgccc cgcatgaagc cgtttgttac cgcctgttta ggcggcgcgg cgggcggttt
    22441 gtttatcgga ttgatagcct ggtggggtct gccgatgggc ttaaacagcg cctttgggcc
    22501 gtctggtctg gtggcgctgc cgctgatgac ttccgcacaa ggcatccttc cggcaatggc
    22561 ggtttatgct ggcgggattc tggtggcatg ggtttgcggg tttattttca ccacgctctt
    22621 tggctgccgt aacgtcaatc tggactgata ttatgaaacg gacaatgctc tatctttctc
    22681 tgctggctgt tagttgtagc gtcagcgccg caaaataccc tgttctgaca gaaggtagtt
    22741 gatcaagtat atgcggcgtt aaagcagtaa taaaaaggcc agtcagtaat gttactgcct
    22801 ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca gcgacggtgc
    22861 gaaataatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac gtttgccatc
    22921 ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg cgcagtaggc
    22981 gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct ggcgaacaat
    23041 cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg tttccggacg
    23101 ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca tctcctgatc
    23161 gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca caaacacata
    23221 gctgccgccg gaatccacgc cgtctttgat aaccgccact tcgcggcgcg tctcttcacc
    23281 cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca cccaacggaa
    23341 gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg ccgcctgagc
    23401 gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat cgaactgggt
    23461 cgtcggcgca aggcctttac cgtagcccgg aaaatctttc agctcttctg ccccaacgcc
    23521 gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa gatgcgcgtc
    23581 cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg ccgcacgcag
    23641 ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc aatgttctgg
    23701 caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataagaatg ccacgcaatc
    23761 cgtggcattg ttgagcgcta ttttacgtgt tttttcgcga cggcggtttg ttcagacgca
    23821 aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc gtccgaaggc
    23881 attaaccctt caggaccacg ccattccccg ctgaactggt agctaatatg ttgactgcct
    23941 tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca attgccaaat
    24001 gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc agcaggaata
    24061 tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat caccatcgcc
    24121 acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc ggttttcatt
    24181 ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc ttgcagtggt
    24241 gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggacga tccaaaccag
    24301 ttccacgggt tagcggcaga ccagttaact gacgacatcg tggaacagcc ggtcagcatc
    24361 aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt ggttattcaa
    24421 taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc ttcattaaaa
    24481 caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc cgccactacc
    24541 agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg ttgccagcaa
    24601 agtaaaacca cctgcgccaa cagcaacaga aaatagagca catgccaggt gcgaggaaac
    24661 gaagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaataccggg aatcagcccc
    24721 agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc ctgctcacgc
    24781 gacgcaccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag cacaagccag
    24841 aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg gaactcagta
    24901 ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt catatcccag
    24961 gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct gaattttcgg
    25021 gcagccgcga gcaatcagct ttttctccag ccggttaagc aacgcattgg caattccacg
    25081 cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc cgtcataacc
    25141 gcccatcacc gttccgacca cttcaccgtt tacctcagcg accaaaaaca aactgacgtc
    25201 atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca gcaagtcgca
    25261 acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc gtatctccat
    25321 ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg tcgaattagc
    25381 caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca actattgaaa
    25441 tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga tacgatataa
    25501 catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc actaaaaaca
    25561 ctcacttcgc gccgccaggt gctgaaagcc ggtttagctg ccctgacgtt gtcaggaatg
    25621 tcgcaagcca ttgccaaaga cgaaccttta aaaaccagca acggacacag caagccgaaa
    25681 gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg cggaattgat
    25741 accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc gattgctaaa
    25801 aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg ttctggcgat
    25861 acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc agatctgttt
    25921 atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc ggtatttgcc
    25981 ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg cgaaaaccgc
    26041 gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca gcaagtactg
    26101 tttgatctgg tgcaaaccga tactattaaa aacagcctga cgcttggctc gcatattctg
    26161 aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc cgcgtttgtg
    26221 gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat caccaacccg
    26281 gaagaagagc ggctgttagg cacggcggcg tttcgtcaga aaatcgccac agcgattgct
    26341 gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc gaaaaagcga
    26401 taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct tcaggatacg
    26461 atttgtcagc agctgaccgc cgtcgatggc gcagaatttg tcgaagatag ttggcagcgc
    26521 gaagctggcg gcggcgggcg cagtcgggtg ttgcgtaatg gtggtgtttt cgaacaggcg
    26581 ggcgtcaact tttctcacgt ccacggtgag gcgatgcctg cttccgccac cgctcatcgc
    26641 ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt gcatccgcat
    26701 aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt ttattgccga aaaaccgggt
    26761 gcagagcccg tttggtggtt tggcggcggc ttcgatttaa cccctttcta tggttttgaa
    26821 gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gccagccatt tggtgaagac
    26881 gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca tcgcaacgaa
    26941 cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt cgaccactgt
    27001 tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc aattgtagag
    27061 cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta ccgtcgcggt
    27121 cgttatgtcg agttcaatct ggtctgggat cgcggcacgc tgtttggcct gcaaactggc
    27181 gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga gtatgattat
    27241 cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt cagggattgg
    27301 gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca acgtcaacga ctgcttctcg
    27361 gcaaacagct gctggtaatc cgtggcaaat tgccccagat gccagaatcc ccactgcatg
    27421 gcggcgtctt ttaccgttgt gctttgcgac cacgggctta tcagttcgcg gcgtacggcg
    27481 ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc gtgaaacgcg
    27541 ttttgtagcg tgcggcgact gacatgcagt tgattacaca aatccagcac cgtcaccggt
    27601 tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg gtaactctga
    27661 tgactgatgc tttccgccgt caccattggt tgcgcttctt ccagcatcgc ccccatcgcc
    27721 attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt ttccgggttc
    27781 tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt atgctgctct
    27841 ttcacttcca gcgccgactg gctacgcagc atatgtaata cccgatccgg attgtgcaaa
    27901 aagttagcct gacaggtgat gacatcttca gacagcacta cgcccaggat cgtataatcg
    27961 tccggcgtac ttaattcaaa ctcggttcca ccggggcgcg tcgctatttc tgcgcttccc
    28021 agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc aaaccagaac
    28081 gagttcggcc agaccaggca cgactgacgc agcgccagac cggtgtattc acgaaaaacc
    28141 tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg atgcagctga
    28201 tcgtaaatct gctgctaggc ggtaatcgtc aaagcatgtt catagacatc cgttgtctgt
    28261 cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc gggtaagggt
    28321 tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt aatgccgggt
    28381 gttgtaggac acccggcacc tccgacaggt taatggggct tgagacgata acgactactg
    28441 cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc tttttccagc
    28501 ggccagccaa agtgggcggc cacttctcct gccgtcattc cctgacatac tgatgttaac
    28561 agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg ttcctttacg
    28621 ggttgcttcg gctggcggtt aaagccagta accagccact gggtgtcatc gtctggccga
    28681 ccgatctcct tacggctgat gacacgcccg gtacgcatcg cggcagcaca acctgcgtcc
    28741 agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaagcg accagggtca
    28801 agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc cgctgcatct
    28861 atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat cggcaacctc
    28921 cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt aaaggcgtta
    28981 caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta gttggtttca
    29041 gacggtggcg ggacgtaggt caccagctgt acgtcggcag atttcaacgc ggcatcaatg
    29101 ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc aagcgtgatc
    29161 ccggcggttg atgaaagata agaaccggta cgcgaaacca catgcgccag gaaggcggtg
    29221 ttttctgcgt cattggccca ctggaaagct gcgccatttt caatatgtgc aaccatcgca
    29281 tccagaccgg cacgcacttc cgccgggttt ggcccaccaa gcataatcag cacttcaccg
    29341 gcagtcggtg acgggccgtg tgccgctccc gcatacagcg agcgaccata taccacttcg
    29401 accatcgctt gtttggtcgc ttcgtcagcc gcaatgtagg ttacgtcgtc agaatcggca
    29461 gaaatcagcc cgaggctacg aatatgcggc ggcaatttta gttcacgcgc aaattcagcg
    29521 ttaacggagg caatcacccg catggcggtt accgacggtc gaatcaaatc taaagctggc
    29581 atgatgcctc cttatcgggt catgttgatg ccggatgctt tctgctccag catacgtttc
    29641 gccaaatcca caatgacggc acagcagcac gaaagagaag caaaagtcag ctacctggta
    29701 cactaggcag cgggtcagca aaggggggca aatgcccctt ttttcatgtc agataatcag
    29761 atcattacct caataaacta ataatcacat cgcggttaca accagtttga tagtgatagc
    29821 tcaaacccag acgtggcgct ggctgcaagc aaaataagcc gcaaaaatgc gaccccatgt
    29881 ttactagccg caaaaatgcg gtcctatttg ccagaacctt ttattattca attggttagt
    29941 gcaagaaaaa gcgtgattac tgttcaagaa gtatccagaa aggtcatgat tttgacgctg
    30001 aaaaaaaata ggtgggcatt tttgcccact cactagccgc aaatttgccc actcactagc
    30061 cgcatttttg cggcccaaag taataaatac agagaaataa taaatacaga tcagcgccaa
    30121 gatttttcgt cttttcggcg gaatcatgag actagtgctt acgtcctgat aacctccaga
    30181 gcagacctta acttctgtgc agcaggacta cgctcattgc cgctcggcta ggtcaaatcc
    30241 tcacgataaa gctgttcgga ttgtgaccat aatgttaaag gactctttaa atcattcctt
    30301 gacgaattaa ctagctggct taagcggaat agcatgcagg ataaaaacaa aaccagctta
    30361 gcagtgggga gagcttcgct tcgctcgtct cctggggcca atgctcaatg aaacgacgta
    30421 aggcacggag cgctcaccat aggtagacac ataaacatct gagagcgcac cttctatcgc
    30481 gctacgcgct caataaaaac caacgacacc gccacactgc aagaccaatg gtttttatcg
    30541 cgtatacaga ccagacagga aacagcagca aagcagcgta tagccaggtg cagacagcac
    30601 actcacacga cgcacagcag gcttaagtga tcactcagga aactttatcc ccgccgccga
    30661 tcaccgcgaa ctatcatgac cgcgccaaat tcgaaaggaa tggcccatag aacgcgatga
    30721 acacacataa gcgctacact tacttaacgt cgccacagag cgattgagag cgcttaaaat
    30781 cgatgcgatt tcattaaatt tttctcactc gcctgaatgg tggcgatcag gactgatcgc
    30841 aacattgccc cgcctcgata ttgagcgcat aatagataca tacattaagc ggaaaagaga
    30901 taagtttatt gtgaaaatca gggggttaca tatcatgcgc atatcagaac aacaacggca
    30961 gatcctcgac tatctggcaa aaaacgaatc aatcaccgca cgacaagccg cagatctcgt
    31021 ttatggtggc aatgttaccc gcgtccaggt tgacgccgcc cgccgctcac tcatgacgat
    31081 ggttagtaat gggctaatca ggaaacaagg ccgcgctttc gtagctgaaa gacatatcaa
    31141 tcacgcttat agccaggacg ttgaagaatt gcgccgccag atcctttggg tattcagcaa
    31201 acagggtcag gagtatttcc tttctgaaca cccaaatttc gccgccaaag agttaacagt
    31261 aagcggattg tcgtgcgcac tggattatct tgtcggtgaa ggttgcgtta cttacaagca
    31321 gctatggcac gccctgaata cgcttgtaga agatggttta ctcattgcca cgcgtgagaa
    31381 catcgaatac tggataatgc cgcgttcagg tcgccgcaat catgcacggt atatttcagg
    31441 caacagatac agaacgccgg actttaatcc gacacaggaa gatcgggacc gtgccgaaat
    31501 gatgtgcagg gaacaggaga aaagaattat ggataagtta ttttcgatgc agtgaacaca
    31561 agcgggcgca atgcccgttt ttattgcctt attccacgcc tgacgaatca atttgatcac
    31621 acctgagaaa atgcatagcc agaagttaag gccgttatat tgcgattggg agcgttttaa
    31681 acggtatcaa tttaattaat tctgatgtgg tgatttgcag gcaaaaaaaa cccgccggag
    31741 aagcgggaaa tagagacata tcgttgtagt tattaacaga tcactttgag gtcgagggcg
    31801 gctaaccctc gctaatatta tcccgctatt accttgcggg tgcaaataat cttttattgc
    31861 tttgatgatt taataaatag cactttttgc taacaaaaac tgctgtcaat atatggccaa
    31921 aaatgtcaac agaaaaaata aggtttataa tattaagtat cacatgggta atgggttcag
    31981 ggaatcggaa tccaccgcct cacggaaatt caaaatttgc ttcttctttc ctccgtgggc
    32041 ggcttctttt tcgtatcgag gtatttatga aagcaataac attagaacag ttcgcagcaa
    32101 tggcgaatgt atcgaccaga acgctgaggc gctggctaaa aaaaccatca accggagtcc
    32161 ctttgccatt taccccacgc ggtactcgcc aattattctg ggagcacgaa tgcaaagaat
    32221 ggttgtacag aacagaacga taataatcac gcatcacatt ttgaggtaaa catcatgacc
    32281 aatatcgaaa gattagaacg ctgtgccgat attatgcggc gcagatggat ttatgaccct
    32341 gaagcgggtt tattgtttag ccgtgaaaca aaagacgtta tcaaaggcag catgaccaat
    32401 aaagggcatt tggttgtaac ggtacatgaa ggtaaattca aagtgacact cacctatcag
    32461 aaagcctgct atgtatacac atacggcgct tatgacgaga cattatatga agtgcaccac
    32521 gttaatttga acaaacagga taaccgcatc aagaatattc gcctgatgcc gaaagaaaag
    32581 caccgccgga tacataaaaa cgtccgcaaa cttattcggc tgggccacac acaatggctt
    32641 caaccgcaat taattgaaca atcactataa acacaggagt aaataccatg cattcattag
    32701 acagttattt tcagcgcacg acagccccta aatcagccgc ccaggaacgc cgtgaggaat
    32761 tccatgagaa agtgatgcgt tctgctgatt atattgcgga caagttcgtg gaaacggttc
    32821 gtccacttgt tgatgaagta gctgataaac ttcagtctga aatgccggaa gatatggaag
    32881 gcactgcaaa gcggcgactc atttgcgaat taagtcgtcg ttttggcgtg tcaataagcg
    32941 cttttaaata atcgaaaaaa ggggggaaag ataatggacg catttgaggt actggcaaat
    33001 aaaggcgctg aactggtagc acagcgtgat aaaaccgcca acgaagggga acgcgcactg
    33061 ctaaataaac agattaaagc gatccgtatg gcccagttta aattaatcag taatgaagtt
    33121 atcgaaacaa ttaaaccgca gatcccacaa atcgttgctg acgctattaa agccgccgga
    33181 ctggagaaac gcattgccgg gctaaaaacg aacgcagaga aaatggagat gcacaaacag
    33241 agcggtgctc cgaatcccga tgagtacttc tcaccggatg ttgagtttat ggcgcaggtt
    33301 gaggaacgtc tgggtagctg tctcaccgag gaacagcgtc gctattttga tggagtggat
    33361 agttcggcag gtattgacct gaactcgtat ttcggtcgtg aaattgagca ttttgatgcc
    33421 gccccgtcta tgccggagcc agaaccagaa atggccaccg atcctgaagc tgagcaacgg
    33481 aaacgcgtat tcaatgcgat agggattcgg tactgatcgg ataaaagaaa gccgggaatt
    33541 accccggctg acttaacaat gtgaccaatg aacaaatcgg acaagacaaa caggataagc
    33601 agtctcgaaa caatgttcgg gaactatatt agcacaagtc cggagaaaat catcatgttg
    33661 gcactacgca tcatgcaggg catcgctaag accctcgcag aacatgtctt agacctgaaa
    33721 cattcgccac tgagcaaaca ggccatgaag cgccaaacgc ttcgtctgtg ggctgaatat
    33781 tcgcttggaa ctatcaataa aatcatcgac atgaaatcgg ggccgagcaa ccagagcgcg
    33841 gaggaaatgg agttcatcag gcgtttaatt ctcattcgca gagacattca tagccagtta
    33901 cacagcgtgg gaatcgatat taatgatggg acgggcgatt aattttcgga caagtactat
    33961 gcgcgcgcga ggagctgaac atgaagcaag atattgcaga caggctggag atactcgaag
    34021 gccagcgggc agaagcgaaa caactacgta agcaggccag aagagcgcac aggaacaacg
    34081 aagcagagtt actaaccaaa tacatcagtt tcaccaatta ctgcatctac gagtgttaca
    34141 aggaagatgc agaggactgg ctggattcat tgccagaaca gtactgagca gaataacacc
    34201 acgtcatgtt gttctgaggt gagcatatca cctctgttca ggtggccaaa tttagtgtgc
    34261 cacttcagca tcaccgttga aatcacaata acgatttttt tgtaaatatg ctctcacata
    34321 tgtaggaata gaccaggttg gcattgaaac acatctacca tgaccataat aaagtaattt
    34381 tttgatatgt ttatggtttt atattaatta aaatatatct aaaattgata gttttaaaat
    34441 tgcaaaatcc agattgtgtt tgtaatgttt aatctgattg tattgagtgg cgtttattat
    34501 gaaaaataaa tcaggtaatt cttgttatag agaagcagtc gatattcata gctcacttga
    34561 gctttcattt atattgcata aatatcattt gcgtgtagat gagaagaaat ctcgaaatgt
    34621 attcagcgcc atacgtgatg agctcatcct tgatggggat tcacgacaaa acttagctac
    34681 tttctgtcaa acatggcttg atgatgaaat ccgtgagttg cttaatttag ctattgataa
    34741 aaacatgatt tataaagatg aatatccaca aatggcggaa cctgaaggtc gctgtgtgcg
    34801 tatgctttcc gattcgtgga gtttccccga ttcgcgacac accttaggtt gctcaaccat
    34861 tggttcatct gaagctgcta tgctcggccg cttggcgtta aaatggcagt ggtgtaaaaa
    34921 acgcgaagta caaggtaaat caaccgaacc tgatttgcga tcctgtgcaa atatgctggc
    34981 ataaattctg ccgttactgt gatgtgaaat tgtgtgaaat tccacttgat gatcatcacc
    35041 tgataatgaa tgctaaagag gttttgaagc atgtcgatga aaacactatt ggtgtcgtta
    35101 cgactttagg tattacttat ttttgtaaag cagattgcgt tagtgttttt aagataagtg
    35161 ttaaagttaa tattagtttt tatatttaca tcgtaattat atgtttctat tttaaaaatg
    35221 ttcgagagta taaaaatgtc taatgctaat atcgcaccca aaagtaagaa aatgacagtg
    35281 gggacactcg ctatcatgaa tataactgct gtagtaagtc tacgatttat accttctgaa
    35341 gcagaatacg gcttaggtgc cattttttac tatacctttg ctgcaattat gtttttagta
    35401 cctgttgcct ttgttgcggc tgagctagct acaacatatc cacaaaaagg gggggcattt
    35461 cgatggatag gagaagcatt tggggctcgc tggggttttg taggaatgct tatgacctgg
    35521 ctacaagtca tcccctactt tccaacagta ctgacatttg gtgcagtctc ggttgcattt
    35581 attgatgttg atatgggggt ggcagaatca attgccagta ataaatggta tattttattt
    35641 ttcgtactat ttgtttactg gggagctatt tgtgttgcat tacgtggcgt tggtattttc
    35701 tcgcaggtat ctaagtggtg cggtattata gggacaatta ttccagctat tatccttgtt
    35761 attcttggtt tctcctatct gtggttcagc ggtaaaccag cggtgatccc attaggttgg
    35821 ggtgatcttt tccctaatat agctaacttc cagaatattg ttcttgccgc cagcatattt
    35881 cttgcttatg cagggatgga aatgaatgcg gtccatgtta acgatttaga taatccaact
    35941 aaaaaatacc caattgcaat taccatcgct tctataggta ctattgcaat attcctgctc
    36001 agtactttag gggtcgcctt tattattcct acagataaaa ttagtctaac tcaaagtttg
    36061 ttggttgcct acgatatgct gttcgaatgg gctggagtgc catggcttgg ctctgtgatg
    36121 gcatttatgc tagcaattgg cgtactgggt ggggtggtta cttggattgc agggcctaat
    36181 acaggtgtat tagcgatcgc gaaagctggt tatttaccta agtttttcca aaaaaccaat
    36241 cgtcatggaa tggggcacca tctaatgttt gtacaaggta taattgtctc agttctttct
    36301 gttacttttg tgataatgcc ttcagttcaa gcagcttttc agattttatc acagttaaca
    36361 gttatgctct atttagttat gtatatgctc atgttcgcta gtgctatcta tctacgacat
    36421 tctcaacctg atacaaaacg cccatatcga gcccctgctt tatttttctg ggcaacactt
    36481 ggattcttag ggtcttttct ggcttttgta ttaagtttta ttccgcccga gcaaatccca
    36541 actggtagtc caaccagcta tgttctttat ttgttagtat tagttgccct atttttatta
    36601 attccaatat taatttacgc cttccgaaaa tctgactgga aagatccaaa ctctgatttc
    36661 gaaccatttt cttgggaaaa aagaactagt aattaaattt tccataagct acaaaagatg
    36721 aggttaaata tgtttagtgc tgaatctcca attggtgtaa tagactcagg tgtaggtgga
    36781 ttgacatcgg taaaggaaat aattaatttg cttcctggtg aagatattat ctattgtgga
    36841 gataatatga atgcacctta tggcaaccgt tctgccgatg atatcatcag tttgactaaa
    36901 aaaatgctga cttttttaca gtcaaggaat gtcaaactag ttgctgtcgc atgtaatact
    36961 atatcctcaa ctttggaatc cgaagaatac agcggatatg ctaagagctt tccgtttcct
    37021 atcttatcca tcattgaacc tgcagtagaa gatgtgattc gccagcaata taaaaatgtt
    37081 ggtattatcg ctactgagtt tactattaaa acggggtgtc acaaagagct aataaaaaaa
    37141 ttgaattcaa caataaatgt atttggagaa ccaagcaaaa atcttgcaat gttaattgag
    37201 gaaggtaatc ttaatgctcc tgcaatcttg aatgacatca aaaaacatgt taaccattta
    37261 atttccctcc atccagtaaa tgaaattatt ctcggctgta ctcactatcc aattgttcag
    37321 aatttttttg aagcagttgc accggatata aaatttatta atcctgcaca tgatcaggct
    37381 atctctatta aaaatcactt gggacagcta aatttattaa actcctcaaa tataggaact
    37441 ttgcaaataa atactagtgg aagtatggag atctacaaaa cggtactagg ggaattgagc
    37501 attacgaaac ctcatacatt cagtatcagg cagttttaaa tttatccggt gcaccgtaaa
    37561 atcccgtcct tctgaactgc accccaaaag ttggacacca actgagtaag gtgcatttta
    37621 tggacaaacc cagttacata ctgggtagtg catccaatta gtagaacatg tgtttttcga
    37681 taaacgctcc gatcactttt tcgtggatct caactgagcg tgagaagcag attgttttac
    37741 gacccaaccg cttaatgcgg gtgcgtagcg tcagattatt acgctcaatg cgttgggtga
    37801 atattttgcc ggtcagatgc ttattcttcg gcacctcccg gccatagctg ccccagtcat
    37861 cgctggtcag catgccgatg ttgaagggtg taagcagtgc cagtagctcc cggcacgttt
    37921 gatcggttcg gggaccaaaa gtgtaggcca gtacaccgcc tgttttggtg ttgtacgcgt
    37981 acccgagcca atgttgccgg gctttactgc caacgtagct ccattgctca tcaagctcgc
    38041 agataaacgc cacatcagca tgggcaacag gcgaagaagt tattcgcttt ggcgcgagtt
    38101 ttttaaagtc cggatgacgg tgttactacc aattttcagt gtcctggcgg tatcgcgaac
    38161 cccggcacca ttaaaggcca tttcagttat cagctctttc atacccggct tacgtgcttg
    38221 ataagtataa gtgaactgaa acacacggtg gcagtcacgg cagcgaagtc tgtcacggcc
    38281 tttagggttc tgaccatggc ggtaaacctg agctgactga caacggggac aatgaatatt
    38341 aacgctggtc atgagataac ctcaaaagcc cgtattatac atcagattca actaattaga
    38401 ggcatcaccc atccagcatt ataaaacata ctaacaataa ctaaattata ataatcacaa
    38461 cttacaatca aataataaat gcataagatt aacaatgatt ttttcgggtt atatttatct
    38521 aaaaaagcag aatattatgt aacttattat tttctaagcg aaaatttatt gtgcggtcag
    38581 actaacaaac ttgcgtacca cccgaagaac atgcgaagat ttaagtattc tttctttttt
    38641 ggtttttatt tttcattttc tcttttcttc tctttccggt tctgttctgg ttttggtttt
    38701 ttccttttta tttttattct ttcttcaggg ttatgctaaa acaccaaaac atttttcacc
    38761 acaaaagcat ccaatcatct tgccagacca atcaccttaa ccggatacac tgccggatat
    38821 aatcccacta acacccagac accaagcacc atacctgata actaccgtat tcaggaagga
    38881 aatgcgagtg atttcaccgt caccggcaca accgatccgc caaaaagagg cgtaccaatg
    38941 tcgatatagt cccccgcgcg gacaatcact tcgtcaatga ctgccaacgg gagttgttgt
    39001 agctgtgggc gcaacaacat gcccagagct ttaccaaagt cctgcccggc caccaccagc
    39061 aggggatgcg gattcggaaa acgcgcgacg aaatcgacca gcgcgttgat gaccgtcaga
    39121 accgcggcgt aacgcacagg cagcgaggcg ggaagcgcca gcacgtacac gtcagttttg
    39181 ggatcaagat ccagctgaag cagcgcctgt tgccaggcac tcaccagatc cgtttcatca
    39241 atcgggatcg ccaccggcaa attgcgcagc ggcagttgta cgccctccag ccagattgtg
    39301 ctgccagaga gcgaaagggt atgtgcaccc gcgccaatca ccgtggagcg tacggtttgc
    39361 gccggaaact gcacattcat ctcacgcagg cgcggatggt catgcagcgc cgttgccaga
    39421 agtgggccaa tatcggcaaa acagaacggg ttggcgggct ggtggcgata acattcgccc
    39481 acgcctccag aaagcgtaat gatttcgggc gtaacacctg cgggcagcaa accggtttgc
    39541 atcaatgcct gcgcgagcgg cgaaagcgtt ccgtcaatca cttcgacaat cagcgccgcc
    39601 atccgccggg taacctgcac cagctgcgcg ccggtcagcg aacgggcgtc agtgcctgca
    39661 ccgaagcact catccacaat catctgcccc ggtttatgag cgtaaaccac gcgcccctgg
    39721 ctgtcggttt ccagcaggcg accaccgacg ttgagacagg cagtgccgct gatttttccg
    39781 gcatcgaaca gggcgtagtt cgcggtgcca ccgccgatat cgatattcag tacccgacac
    39841 agccgttgtt cagaaagggt ttgcgccccg gctccgtgac cggcgatcac ggattcgagg
    39901 tgcggcccgg cgctggcgac gacaaaatcg cccagcgact gtttttcctt catcactttt
    39961 tccatcagct gtgcgaccag gctttcggta aactggcctt ccggcagctg ggcaatgatg
    40021 gtttcgcgga tgcgctggct ttcggttttg tcggctggca cgctggcggg cacggacgcc
    40081 gttaccggga tcgattcatc acattcggtg atggtaaaac ccagtagatc cgccacttca
    40141 cgggcttccg gggtaataat gctggcgcgc agaaccacgg acattgcctg ttcgccgcgc
    40201 gcgtgtgctt cacgaatatc attcgctgtg atgagttttt tcacctgctt cctcctgttt
    40261 ggtgagagat gccagataat ccaccagttg ctgcacgctt tgcgggtcgt ggctgtttag
    40321 ctcgaaaatc ggctcttcga aacccgtctc aagcaataac tttcgcgttg cggcgacgtc
    40381 agcatctggc atgtccgttt tactgatgac ggcgatttgc cgtttgctga cgccaatatc
    40441 cagcaacccg gcaggcaacc gactttccgg gtcatttgcg ccatggacat agatcagcat
    40501 gtctacatcc tgtagcgtgg taattaaggc gtgataccag cggggatggc taaaatattc
    40561 acccggcgta tcaatatcgc ccttatcatt aaattccacg gccagtgttt ttctggcgag
    40621 ggtataattc ccctgcaacg cattaaataa cgttgttttt cctgccccga cgctacccac
    40681 aaacgcaatt cgtttcatga atgc
//
LOCUS       DBKELA010000024        39819 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.024, whole genome shotgun sequence.
ACCESSION   DBKELA010000024 DBKELA010000000
VERSION     DBKELA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.024"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..155
                     /locus_tag="ACUQR0_001275"
     CDS             <1..155
                     /locus_tag="ACUQR0_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9108395.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACUQR0_001276"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACUQR0_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HHV9108396.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3035
                     /gene="uidA"
                     /locus_tag="ACUQR0_001277"
     CDS             1362..3035
                     /gene="uidA"
                     /locus_tag="ACUQR0_001277"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HHV9108397.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACUQR0_001278"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACUQR0_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACUQR0_001279"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACUQR0_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HHV9108398.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACUQR0_001280"
     CDS             complement(5626..7134)
                     /locus_tag="ACUQR0_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HHV9108399.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACUQR0_001281"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACUQR0_001281"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HHV9108400.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACUQR0_001282"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACUQR0_001282"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HHV9108401.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACUQR0_001283"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACUQR0_001283"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HHV9108402.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACUQR0_001284"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACUQR0_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HHV9108403.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACUQR0_001285"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACUQR0_001285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HHV9108404.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACUQR0_001286"
     CDS             complement(14283..15602)
                     /locus_tag="ACUQR0_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HHV9108405.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACUQR0_001287"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACUQR0_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HHV9108406.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACUQR0_001288"
     CDS             16699..17034
                     /locus_tag="ACUQR0_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HHV9108407.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACUQR0_001289"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACUQR0_001289"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HHV9108408.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACUQR0_001290"
     CDS             complement(17790..19172)
                     /locus_tag="ACUQR0_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9108409.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACUQR0_001291"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACUQR0_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HHV9108410.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACUQR0_001292"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACUQR0_001292"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HHV9108411.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACUQR0_001293"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACUQR0_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HHV9108412.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACUQR0_001294"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACUQR0_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HHV9108413.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            24950..25120
                     /locus_tag="ACUQR0_001295"
     CDS             24950..25120
                     /locus_tag="ACUQR0_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108414.1"
                     /translation="MFNYRGQLSSAMTRSNTADNCNIMDNLFRTLFSTFTHLRTSSTI
                     LLVGEQHWRNAL"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACUQR0_001296"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACUQR0_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HHV9108415.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACUQR0_001297"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACUQR0_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HHV9108416.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACUQR0_001298"
     CDS             complement(25947..26768)
                     /locus_tag="ACUQR0_001298"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine peptidase"
                     /protein_id="HHV9108417.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACUQR0_001299"
     CDS             complement(26868..26951)
                     /locus_tag="ACUQR0_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108418.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACUQR0_001300"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACUQR0_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HHV9108419.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACUQR0_001301"
     CDS             27623..27727
                     /locus_tag="ACUQR0_001301"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HHV9108420.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACUQR0_001302"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACUQR0_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30038
                     /locus_tag="ACUQR0_001303"
     CDS             29136..30038
                     /locus_tag="ACUQR0_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9108421.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="ACUQR0_001304"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="ACUQR0_001304"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HHV9108422.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="ACUQR0_001305"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="ACUQR0_001305"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HHV9108423.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACUQR0_001306"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACUQR0_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HHV9108424.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACUQR0_001307"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACUQR0_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HHV9108425.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACUQR0_001308"
     CDS             complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACUQR0_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HHV9108426.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACUQR0_001309"
     CDS             complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACUQR0_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HHV9108427.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="ACUQR0_001310"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="ACUQR0_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37857..38891)
                     /locus_tag="ACUQR0_001311"
                     /pseudo
     CDS             complement(37857..38891)
                     /locus_tag="ACUQR0_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            39192..39749
                     /locus_tag="ACUQR0_001312"
     CDS             39192..39749
                     /locus_tag="ACUQR0_001312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108428.1"
                     /translation="MRQRELAATAKAFLLDNPEVTLQELTAHIRSMAEQFLTDASDDY
                     WNGVEVATLVDEKSNLRELAATRALSVDQQRGIKLALEVLTAAQQRVDTGDTSGLIKL
                     IDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRPAVFTQERQLLTPESRESSVLFQE
                     LVTSLLAEKVQTLKQSSFKDLSASL"
BASE COUNT        10133 a         9651 c        10194 g         9841 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaat actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaatatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtgg
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgccag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattaag taaattcaat tttacaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccacgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgtggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctag cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgactagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtga tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct gccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcta ctgtcagacg gatggacgga cttgaagtag ttgactcaac gctacttaag
    38941 gtcctgatat gactgacatt aaccatgacg atactgcata tccatccagt tctttctctg
    39001 ggtctcccta tctgtatcag caagtctaca cctcaagtcc tgacacaagg ctgacacaaa
    39061 tcaaggacct tgctacgcca cagctagtcc ggagaaccaa cgggcgttac actgtccgct
    39121 tcagacttaa aggagctacc agcccgtttc tatcagtctc aacccgcagc acagaccgga
    39181 ggaccgctac aatgcgtcag agagagctcg cagccacagc taaggcattc ctactggaca
    39241 atcctgaagt cacactacag gagcttacag cgcacatacg gtccatggct gagcagttcc
    39301 tgactgatgc cagtgatgac tactggaatg gcgtagaggt ggcgacactg gttgacgaga
    39361 agtccaacct cagggaactt gcagcgacaa gagcgttgag tgtggaccag cagaggggca
    39421 ttaagttagc gctggaagtc ctgaccgcag cacagcagag agtcgacact ggtgatacct
    39481 ctggtctgat taagctgatt gatggtggcg taagccagct tactgatgat aataaccata
    39541 ctgattactc aactataggc gactctacgt cgattctgaa gaatgagcag ggggatagac
    39601 cagcagtttt cacacaggaa cgtcagttac tcactccaga gtcacgagaa tcatctgtac
    39661 tttttcagga attggtgaca tcactgctgg ctgagaaggt ccagacactg aaacaatcca
    39721 gctttaagga cttgtcagcg tccctgtgaa ccgccccggg tttcctggag agtgttttat
    39781 ctgtgaactc aggctgccag atcatcgttt ccgatggaa
//
LOCUS       DBKELA010000025        39232 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.025, whole genome shotgun sequence.
ACCESSION   DBKELA010000025 DBKELA010000000
VERSION     DBKELA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.025"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(20..907)
                     /gene="rpnA"
                     /locus_tag="ACUQR0_001313"
     CDS             complement(20..907)
                     /gene="rpnA"
                     /locus_tag="ACUQR0_001313"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HHV9108429.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
     gene            complement(1110..1346)
                     /gene="feoC"
                     /locus_tag="ACUQR0_001314"
     CDS             complement(1110..1346)
                     /gene="feoC"
                     /locus_tag="ACUQR0_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HHV9108430.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1346..3667)
                     /gene="feoB"
                     /locus_tag="ACUQR0_001315"
     CDS             complement(1346..3667)
                     /gene="feoB"
                     /locus_tag="ACUQR0_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HHV9108431.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3684..3911)
                     /gene="feoA"
                     /locus_tag="ACUQR0_001316"
     CDS             complement(3684..3911)
                     /gene="feoA"
                     /locus_tag="ACUQR0_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HHV9108432.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4349..6670)
                     /locus_tag="ACUQR0_001317"
     CDS             complement(4349..6670)
                     /locus_tag="ACUQR0_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HHV9108433.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6767..7243)
                     /gene="greB"
                     /locus_tag="ACUQR0_001318"
     CDS             complement(6767..7243)
                     /gene="greB"
                     /locus_tag="ACUQR0_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HHV9108434.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7471..8190
                     /gene="ompR"
                     /locus_tag="ACUQR0_001319"
     CDS             7471..8190
                     /gene="ompR"
                     /locus_tag="ACUQR0_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HHV9108435.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8187..9539
                     /gene="envZ"
                     /locus_tag="ACUQR0_001320"
     CDS             8187..9539
                     /gene="envZ"
                     /locus_tag="ACUQR0_001320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HHV9108436.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9615..11237)
                     /gene="pckA"
                     /locus_tag="ACUQR0_001321"
     CDS             complement(9615..11237)
                     /gene="pckA"
                     /locus_tag="ACUQR0_001321"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HHV9108437.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11616..13340
                     /locus_tag="ACUQR0_001322"
     CDS             11616..13340
                     /locus_tag="ACUQR0_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HHV9108438.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(13484..14353)
                     /gene="hslO"
                     /locus_tag="ACUQR0_001323"
     CDS             complement(13484..14353)
                     /gene="hslO"
                     /locus_tag="ACUQR0_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HHV9108439.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14378..14779)
                     /gene="hslR"
                     /locus_tag="ACUQR0_001324"
     CDS             complement(14378..14779)
                     /gene="hslR"
                     /locus_tag="ACUQR0_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HHV9108440.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14790..15458)
                     /gene="yrfG"
                     /locus_tag="ACUQR0_001325"
     CDS             complement(14790..15458)
                     /gene="yrfG"
                     /locus_tag="ACUQR0_001325"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HHV9108441.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15523..17658)
                     /gene="igaA"
                     /locus_tag="ACUQR0_001326"
     CDS             complement(15523..17658)
                     /gene="igaA"
                     /locus_tag="ACUQR0_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HHV9108442.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17978..18538
                     /gene="nudE"
                     /locus_tag="ACUQR0_001327"
     CDS             17978..18538
                     /gene="nudE"
                     /locus_tag="ACUQR0_001327"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HHV9108443.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18703..21255)
                     /gene="mrcA"
                     /locus_tag="ACUQR0_001328"
     CDS             complement(18703..21255)
                     /gene="mrcA"
                     /locus_tag="ACUQR0_001328"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HHV9108444.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21375..22154
                     /gene="hofM"
                     /locus_tag="ACUQR0_001329"
     CDS             21375..22154
                     /gene="hofM"
                     /locus_tag="ACUQR0_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HHV9108445.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22154..22693
                     /gene="hofN"
                     /locus_tag="ACUQR0_001330"
     CDS             22154..22693
                     /gene="hofN"
                     /locus_tag="ACUQR0_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HHV9108446.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22677..23117
                     /gene="hofO"
                     /locus_tag="ACUQR0_001331"
     CDS             22677..23117
                     /gene="hofO"
                     /locus_tag="ACUQR0_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HHV9108447.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23107..23511
                     /gene="hofP"
                     /locus_tag="ACUQR0_001332"
     CDS             23107..23511
                     /gene="hofP"
                     /locus_tag="ACUQR0_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HHV9108448.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23423..24661
                     /gene="hofQ"
                     /locus_tag="ACUQR0_001333"
     CDS             23423..24661
                     /gene="hofQ"
                     /locus_tag="ACUQR0_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HHV9108449.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25062..25583
                     /gene="aroK"
                     /locus_tag="ACUQR0_001334"
     CDS             25062..25583
                     /gene="aroK"
                     /locus_tag="ACUQR0_001334"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HHV9108450.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25640..26728
                     /gene="aroB"
                     /locus_tag="ACUQR0_001335"
     CDS             25640..26728
                     /gene="aroB"
                     /locus_tag="ACUQR0_001335"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HHV9108451.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26820..28106
                     /gene="damX"
                     /locus_tag="ACUQR0_001336"
     CDS             26820..28106
                     /gene="damX"
                     /locus_tag="ACUQR0_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HHV9108452.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28213..29049
                     /gene="dam"
                     /locus_tag="ACUQR0_001337"
     CDS             28213..29049
                     /gene="dam"
                     /locus_tag="ACUQR0_001337"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HHV9108453.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29067..29744
                     /gene="rpe"
                     /locus_tag="ACUQR0_001338"
     CDS             29067..29744
                     /gene="rpe"
                     /locus_tag="ACUQR0_001338"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HHV9108454.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29737..30495
                     /gene="gph"
                     /locus_tag="ACUQR0_001339"
     CDS             29737..30495
                     /gene="gph"
                     /locus_tag="ACUQR0_001339"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HHV9108455.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30488..31492
                     /gene="trpS"
                     /locus_tag="ACUQR0_001340"
     CDS             30488..31492
                     /gene="trpS"
                     /locus_tag="ACUQR0_001340"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HHV9108456.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31762..32667
                     /gene="yhfZ"
                     /locus_tag="ACUQR0_001341"
     CDS             31762..32667
                     /gene="yhfZ"
                     /locus_tag="ACUQR0_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HHV9108457.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32684..33046
                     /locus_tag="ACUQR0_001342"
     CDS             32684..33046
                     /locus_tag="ACUQR0_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHV9108458.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33130..34293
                     /locus_tag="ACUQR0_001343"
     CDS             33130..34293
                     /locus_tag="ACUQR0_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HHV9108459.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34294..35520
                     /locus_tag="ACUQR0_001344"
     CDS             34294..35520
                     /locus_tag="ACUQR0_001344"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHV9108460.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35517..36395
                     /locus_tag="ACUQR0_001345"
     CDS             35517..36395
                     /locus_tag="ACUQR0_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HHV9108461.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36406..36759
                     /locus_tag="ACUQR0_001346"
     CDS             36406..36759
                     /locus_tag="ACUQR0_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HHV9108462.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36771..38075
                     /locus_tag="ACUQR0_001347"
     CDS             36771..38075
                     /locus_tag="ACUQR0_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HHV9108463.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38087..39172
                     /locus_tag="ACUQR0_001348"
     CDS             38087..39172
                     /locus_tag="ACUQR0_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HHV9108464.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
BASE COUNT         9100 a         9902 c        10760 g         9470 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctactcgc cgcagccaac tcttcttctg
       61 acagcccggt aaatcgcatg atgtctgcaa gagggactcc ggattcaagc attattttgg
      121 ctatatgcag ggctttggat tgttccccct cctgccgcaa tctttccgca atagtcatta
      181 aactctcctt gtgtttcggt gaacgttcgg caacgccgtc gataaaatcg ttaaaacgta
      241 cagcgtcgcc agtttgcagt atgtaattaa acagcccttt gatttgtctg tcattagcgt
      301 atccactact taataagcag gccatttgct ctaccagccc catcagatcg cgttgacgaa
      361 tatgtttttg aattaactcc agcagcgcca tacgtcggtg ctgcatgatt tcatcatcag
      421 gcatgacggt gacatcaatc agcggaaatg cggaggcata aagctgcctt gccagtttgg
      481 gatcggcgaa acaatccagc caacacagcg aatagggata ggggctttca ataccgtggt
      541 aaaacaacaa tggcaccacc atcggcaacg ttttgtatcc agcatcaagg tgattttgca
      601 ttgcggcaat agcgtaacgc atcatgcgaa atgcgatcag tttgtttgag gtgctttgat
      661 gttcaatcag acaatagatg tatcctggtc cttgttccgt tttcaccgac cacagcacat
      721 cggaatagct ttcacgcaga tcgtcatcaa taaagctcct cgactccagc ttgagggttt
      781 tcatatcaca gagcgcgtga atgggtgccg gtaaatgaaa cgcaagaaaa tcgcgtgccg
      841 tctccggttg gcgtaaaaag agtttgaaca gcgcatcatg tggggtggaa ctctgctttt
      901 tactcatggc gttccttcac ctgttgtttg aatgccgcga ctctacgcgc cactccacat
      961 ctgtgcatta gcgagttttc atccctgcga gacgctttca gaggaactta cgttgcgctg
     1021 caagaattgc atttttatgg aaagccgcac gcaaaaaagc ccggatgcaa acgcataccg
     1081 ggcttttcaa aacaggcgat ggagtatggt taacgcagcg cccaccactc gcgcagacag
     1141 gctttgcctt ccgggcagct tttacaactg ccagagaggc agccgtcagg ttcttcctga
     1201 atccgcacgg ctttgcccat gctttccagc tgttgcagca tggcgttaat cattggctgc
     1261 ggagtgttca atgtctggct tatctgggtc gcttccatac ggccccgtaa cgccagcaaa
     1321 tcacgtacct gaataagtga agccattaat ggcaatcacc ggtggtgctg gctgcgcagc
     1381 aactgcttac cgacttgcgg gttgccagca gttcgatatc cacccggcta cgcgcgcggc
     1441 gcagcagacc gatagctacg atgttaaaca ggattaccgc cagaatgcac accaggctgt
     1501 aagttgggtg ctgactgtag ctggcgacct gatagaacaa tgttgccagt gagtaagcga
     1561 tattcagccc ccacaggatg gagaagccca tccagccacg gcttgattca cgggcgatgg
     1621 ctcccatcac cgagatacat ggtacataca gcaggacgaa aatcaggtag ctgtatgcgg
     1681 cagctgcgct accgaatttc tgatccatca cgcccatcgc cccggtaccc atttcaccgt
     1741 tgcctttgct agcttcaatg gggttcatca gtacgctaag gctgaaggtg tctttcaggc
     1801 tctgccaggt ttcatctacc gcactgaaca gctcttcacc gaggttgaat tccgccggat
     1861 tgaactcttc gtcctgaata ttttctgcgg tgtagagggt gttgagcgta cccaccacca
     1921 cttcttttgc cattgcgccc gtaaacaggc caaccgttgc ctgccagtta tcttcatgta
     1981 cgccaatcgg cttgaagaca ggggttatca cccggctgac ggaagccagc gccgagtcgt
     2041 tgatgttatc gacgattttc ccgctcaacg agaagctgtt gaaagcgctc aggaaaatgc
     2101 tgacgatgat aatcacttta ccagcacgca gaacgaagcc cttcagacgc tgccaggtct
     2161 ggataatcag gcttttaacg tgtggtacat ggtagaccgg cagctccatg acaaacggcg
     2221 tcgcttcacc gcgcatgatg gtgtacttga gcatcaggcc agtcagcacc gccatcacga
     2281 taccgagcat atacagcgag aaaaccgcca gggcaccgtt ctgcccgaag aaggcagccg
     2341 cgaatactgc gaagatagcc agacgcgcgc cgcaggacat aaacggtgcc atcatgatgg
     2401 tcatcagacg ttcacgcggt gcatcaagcg tacgtgcgcc cattaccgac ggtacgttac
     2461 aaccgaaacc gacgatcagc ggcacaaagg atttccccgg caagcccagc gcctgcatca
     2521 ggcggtccat cacaaacgct gcacgcgcca tatagccgga gtcctcaagg aaggagagga
     2581 acaggtacat catgccgatc tgcggcacca gtggcagcac ggtgttaata ccgccaccca
     2641 ggccctgggc gaggaagata gtcagccagt ccgggaagtg gagcgtgtag ccaatccatt
     2701 gaataccatg cacaaatagc gccacggagc cgacgttaaa cagcggctgt aacgccccgc
     2761 cgatgttgat agccagcagg aacatcaggt acatcacaaa gaggaaaatc ggcagaccga
     2821 ggaaacggtt gagcacgatt ttatctaccg cagtggtgaa acggctgggt tctgccgtca
     2881 gggtgttgct taccacatca cagatggcag caatgcactg gtaacgcgca tcggcaatgt
     2941 gcagcgccgg atcatccatt tcattacgca gacgggcgag ggtggcatcc agatgctgcg
     3001 acgcttcacc ggcgtaggcg cggctgtaga tatcgccttc cagcatttgc aggcccagcc
     3061 agcgacgttg tttcagcggg atgtcggaag gcatcacttt tgccagtgaa tctgcttcgt
     3121 tgagtagcgg ctgtgcgtaa tgcaccagtt ccacattctc gttagcttta tagcgatcaa
     3181 tcgccagctt gagcgcttca ataccgcgac cacgggttga aaccagcggg atcaccgggc
     3241 agccaagacg tgccgataga gcatcaattt caatacgaat attttgcttc tcggcaatgt
     3301 cgagcatgtt cagcgccacg atgcagggaa tgccgagttc cagcagttgc agcgtcaggt
     3361 acaggttacg ctcaaggtta gacgcatcca ccacgttaat cagcaggtcg gcgtcgccac
     3421 tcaaaatgta gtgacaggcg atttgctcat cgagcgaggt ctgcgatgag atggtggtca
     3481 gagaataggt gccgggcagg tccaccagcg tgacctgatg atcggtggtg gagaattgcc
     3541 cttctttacg ttcgacggta acgccagccc agttacctac acgctgacgt gcgccagtga
     3601 gctggttaaa taaggttgtc ttgccagaat ttggattacc aattaagcca atggttaatt
     3661 ttttcattgt tgttatcacc gtattaacag gaaaccgctt ccacttctaa taaggccaga
     3721 tcttttttgc gtaataccag gctcacacga cgggtttcga tatgaatggg gtcgccgagt
     3781 ggagcgacgc gcaccacatc aaaagaggag ccaggtaaca tgcctagaga aagcagtttt
     3841 tggcgatatg ccgggctgat ttcacgggaa aagccagtga ttttccacgc agtatctgga
     3901 gtgtattgca taggtgccta cttgtttctc attaactgga taactacctc atggggcgtt
     3961 tgaccgcgac atgagaccag atgatttgcc aacaaaggag agattaaata atcaacaaca
     4021 caatgataat gagaatggtt tctatcagca atacttaaaa tgtgtgcgaa tgttggtttg
     4081 aataataatc tgactcaatg ttgatgtggc tcaaggttat atcgcagcga aaatataatt
     4141 cgaatagtga gttacccgga ctggctaatg tttaattaag gtttcagttg ttaatgaagt
     4201 gataaatatt aacggagtat attgtgagtt tggctgtgga ataaaagtgc cggagatatt
     4261 ctccggcact tttatgtcag gcaaagcgtt cgttgccaga tgcggcgtga acgccttatc
     4321 gcgcaggttt aggggccagt ccgggcgttt aacgtttttt acccatcgct gccgccagcg
     4381 catccatcat cgcgctatta ccggcaggct gcgcttcacg accgcgtggt ttggctgccg
     4441 ggcggttgtt ttgcgggcgt tcattaccgc cgccgcgacg agagttggtt tcgccaggct
     4501 gctcgtccag acgcatagtc agggcgatac gtttacgctg aagatccact tccagcactt
     4561 tcaccttcac aatgtcgccc gctttcacca cggtatgcgg atcttccaca aacttgttcg
     4621 ataatgaaga gatgtgaacc aggccgtcct gatgcacgcc aatatcgaca aacgcgccaa
     4681 agttggtgac gttggtcact gcgccttcga ggatcatacc cggttgcagg tcgttcattg
     4741 tctcgacgcc atcggcaaac tgagcggttt taaattccgg acgcggatcg cgacccggtt
     4801 tttccagctc tttgatgatg tcagttactg tcggcacacc gaatttttca tcagtaaagt
     4861 cagacgcttt caggttacgc agttcgctgc tgttacccat cagatctttc agtgcctgct
     4921 gtgttgctgc cagaatgcgt tccaccaccg gataggcttc cggatgaacg gtagaggcgt
     4981 ccagcgggtt atcaccgtgg ttaatgcgca ggaagcccgc gcactgctcg aaggctttcg
     5041 gccccagacg gctgactttc agcagttgct gacggttctg gaactggccg ttctcatcgc
     5101 gccaggccac gatgttttgc gccatcatgc gcgtcaggcc cgccacgcgg gttaatagcg
     5161 gaacagaagc ggtgttgaga tcgacgccaa cggcgtttac gcagtcttct actactgcat
     5221 ccagtttgcg ggccagttgc gtctggctga cgtcatgctg atactgacct acgccgatag
     5281 atttcggatc gattttcacc agctccgcca gcggatcctg caaacggcgg gcgatagata
     5341 ccgcaccacg cagcgaaacg tcgagatccg ggaactcctg cgctgccagc tcggaagctg
     5401 agtaaaccga cgcgccagct tcgctgacga tcactttctg tgcggtcact ttcgggaact
     5461 gcttctgcac gtcgagatag aaacgttcag tttcgcggga agctgtaccg ttgccgatcg
     5521 ccaccagttc aacgttatgt ttttcacaca aggcagcaac ggtcatcgct gcttttgcgg
     5581 cttgtccggt gtgcgggtaa atggtatcgg tcgccaccag tttgccagtg gcatcgacca
     5641 ccgccacttt taccccggta cgcagacccg gatcgaggcc catcgttgca cgcagtccgg
     5701 caggggccgc catcagcaga tcgtgcaggt tacgggcaaa gacgttgatt gcttcatctt
     5761 ccgcgcgttc gcgcacggta cccatcagtt cggtttccag atgcatcagc accttgatgc
     5821 gccaggtcca gctcactacg cttttgcgcc agctatccgc cggggcattg ttcaggcgca
     5881 ggccaaggtg atccatgatg atttgctcgc aatagctctc tttgggcggc tcatcgaact
     5941 gcggatcggc attcagcgaa agctggagta cgccttcgtt acgcccacgg aacatcgcca
     6001 gcgcgcggtg agaaggcacc gtggacaacg gttcgtgatg atcgaaatag tcgcggaatt
     6061 tcgccccttc ctcttcttta ccgctcacca ccgtagaaac caaatgcgcg ttcttccaca
     6121 gataatcacg cactttcgcc agcagcgcgg catcttcggc aaaccgttcc atcaggatat
     6181 agcgcgcgcc atccagcgcg gctttggtat ctgccacgcc tttatcggca tcaacatatt
     6241 gtgcagcggc gacttctggc gtgtgtgacg gatcgctcca cagcaggtca gccaacggct
     6301 caagccctgc ttcaatggcg atttgcccgc gggtgcggcg tttaggtttg tagggcaggt
     6361 agaggtcttc gagttcggtt ttgcttaggg tggcgttgat ggccttcgcc agatcatcgg
     6421 tgagtttgcc ttgctcggaa atggatttga ggatcgcctg acgtctctct tccagctcgc
     6481 gcagatagct cagacgcgtt tccagattac gcagctgcgt gtcatccaga ccgccggtga
     6541 tttccttacg ataacgtgcg ataaacggca cggtattccc ttcgtcaagc aggcgaacgg
     6601 cagcgtcaac ctgttccggg cgcgcctgaa tttcacccgc aataatgcgg cagaacgaat
     6661 cattcatcat ggtttgagtt catcttttcg gatcaaaaat caggggatag ttatacggac
     6721 tggctggcaa aaatgccagc catcggcagg aggttaagac tcttccttac ggtttcacgt
     6781 actcgatagc attaacatac cagttcgctt ccccggcagg ggtattcacc accgccagat
     6841 cgccgacttc ttttttcagc aatgcgcggg ccatcgggga atcgatagag atgtaatctt
     6901 tacggccaaa aatttcatcg tagccgacaa tacggaaacg gtgagtcacg ccatcgtcgt
     6961 tttcaatctc cacccacgcg ccaaaaaata ctttgccttc ctgctgaggg gagtaatcga
     7021 cgattttgag attttccagg catttagtga gatagcgcac gcgacggtcg atttcacgca
     7081 gacgcttttt attatactga tagtcagcat tttcgctgcg gtcgcccaga cttgcggccc
     7141 aggtcacctt ttttgtgacc tccgggcgtt cttcacgcca gagataatta agctcttgtt
     7201 tgagtttttc atacccttcc cgggtaacca ggggcgtttt catctcgttg attccctttg
     7261 tctgtttgat aatgcgcaca ttgggtataa cgtgatcata tcaacagaat caataatgtt
     7321 tcgccgaata aattgtatac ttaagctgct gtttaatatg ctttgtaaca atttaggctg
     7381 aaatttatac cagatttagc tggtgacgaa cgtgagcttt tttaagaata cacgcttaca
     7441 aattgttgcg aacctttggg agtacaaaca atgcaagaga actacaagat tctggtggtc
     7501 gatgacgaca tgcgcctgcg tgcgctgctg gaacgttatc tcaccgaaca aggcttccag
     7561 gttcgaagcg tcgctaatgc agaacagatg gatcgcctgc tgactcgtga atctttccat
     7621 cttatggtac tggatttaat gttacctggt gaagatggct tgtcgatttg ccgacgtctt
     7681 cgtagtcaga gcaacccgat gccgatcatt atggtgacgg cgaaagggga agaagtggac
     7741 cgtatcgtag gcctggagat tggcgctgac gactacattc caaaaccgtt taacccgcgt
     7801 gaactgctgg cccgtattcg tgcggtgctg cgtcgtcagg cgaacgaact gccaggcgca
     7861 ccgtcacagg aagaggcggt aattgctttc ggtaagttca aacttaacct cggtacgcgc
     7921 gaaatgttcc gcgaagatga gccgatgccg ctcaccagcg gtgagtttgc ggtactgaag
     7981 gcactggtca gccatccgcg tgagccgctc tcccgcgata agctgatgaa ccttgcccgt
     8041 ggtcgtgaat attccgcaat ggaacgctcc atcgacgtgc agatttcgcg tctgcgccgc
     8101 atggtagaag aagatccagc gcatccgcgt tacattcaga ccgtttgggg cctgggctac
     8161 gtctttgtac cggacggctc taaagcatga ggcgattgcg cttctcgcca cgaagttcat
     8221 ttgcccgtac gttattgctc atcgtcacct tgctgttcgc cagcctggtg acgacttatc
     8281 tggtggtgct gaacttcgcg attttgccga gcctccagca gtttaataaa gtcctcgcgt
     8341 acgaagtgcg tatgttgatg accgacaaac tgcaactgga ggacggcacg cagttggttg
     8401 tgcctcccgc tttccgtcgg gagatctacc gtgagctggg gatctctctc tactccaacg
     8461 aggctgccga agaggcaggt ctgcgttggg cgcaacacta tgaattctta agccatcaga
     8521 tggcacagca actgggcggc ccgacggaag tgcgcgttga ggtcaacaaa agttcgcctg
     8581 tcgtctggct gaaaacctgg ctgtcgccca atatctgggt acgcgtgccg ctgaccgaaa
     8641 ttcatcaggg cgatttctct ccgctgttcc gctatacgct ggcgattatg ctattggcga
     8701 taggcggggc gtggctgttt attcgtatcc agaaccgacc gttggtcgat ctcgaacacg
     8761 cagccttgca ggttggtaaa gggattattc cgccgccgct gcgtgaatat ggcgcttcgg
     8821 aggtgcgttc cgttacccgt gcctttaacc atatggcggc tggtgttaag caactggcgg
     8881 atgaccgcac gctgctgatg gcgggggtaa gtcacgactt gcgcacgccg ctgacgcgta
     8941 ttcgcctggc gactgagatg atgagcgagc aggatggcta tctggcagaa tcgatcaata
     9001 aagatatcga agagtgcaac gccatcattg agcagtttat cgactacctg cgcaccgggc
     9061 aggagatgcc gatggaaatg gcggatctta atgcagtgct cggtgaggtg attgctgccg
     9121 aaagtggcta tgagcgggaa attgaaaccg cgctttaccc cggcagcatt gaagtgaaaa
     9181 tgcacccgct gtcgatcaaa cgcgcggtgg cgaatatggt ggtcaacgcc gcccgttatg
     9241 gcaatggctg gatcaaagtc agcagcggaa cggagccgaa tcgcgcctgg ttccaggtgg
     9301 aagatgacgg tccgggaatt gcgccggaac aacgtaagca cctgttccag ccgtttgtcc
     9361 gcggcgacag tgcgcgcacc attagcggca cgggattagg gctggcaatt gtgcagcgta
     9421 tcgtggataa ccataacggg atgctggagc ttggcaccag cgagcggggc gggctttcca
     9481 ttcgcgcctg gctgccagtg ccggtaacgc gggcgcaggg cacgacaaaa gaagggtaaa
     9541 taaacgggag gcgaaggtgc ctcccgtttt gctttctata agatactgga tagatattct
     9601 ccagcttcaa atcattacag tttcggacca gccgctacca gcgcggcacc cgcaggggtg
     9661 tcggtgtatt tatcgaagtt gtcgataaac agtttcgcca gggtttcggc tttttcctgc
     9721 cactgttccg gagaagcgta ggtgttacgc ggatcgagaa tcttcgtgtc tacgcccggc
     9781 agttcggttg ggatcgccag gttaaacatc ggcagagtga aggtttctgc attatccagc
     9841 gaaccgttga ggatggcgtc gataatggcg cgggtatctt taatcgagat acgtttgcca
     9901 gtgccgttcc agccagtgtt aaccagataa gcctgcgcgc ccgccgcctg catacgtttc
     9961 accagcactt ctgcgtactg agtcgggtgc agcgacagga acgccgcgcc gaagcaagcg
    10021 gagaaggttg gcgtcggttc ggtgatgcca cgctcagtac cggccagttt ggcggtgaag
    10081 ccagagagga agtgatactg ggtttgatcg gcagtcaggc gagaaaccgg cggcaacacg
    10141 ccgaaagcat cagcagtcag gaagataacc ttagtcgcgt ggcccgcttt ggaaaccggc
    10201 ttaacgatgt tatcgatgtg atagattgga taagaaacgc gggtgttctc ggtttttgaa
    10261 ccatcatcaa agtcgatagt gccatcttca cgcacggtga cgttttccag cagcgcatca
    10321 cgacggatag cgttgtagat ttcaggttcc gcttctttcg acagcttgat agtttttgcg
    10381 tagcagccgc cttcgaaatt aaacacgccg tcatcgtccc agccgtgttc gtcatcgcca
    10441 atcaggcggc gtttcgggtc ggtggaaagg gtggttttac cggtgccgga aaggccgaag
    10501 aacaccgcaa catcgccttt ctcgccaacg ttggcggagc agtgcataga agcgatacct
    10561 ttcagcggca gcaggtagtt catcatcgag aacatccctt tcttcatttc gccgccgtac
    10621 caggtgccgc caatcagctg catgcgctcg gtcaggttaa acgccacgaa gttttcggag
    10681 ttcagaccct gttctttcca ctgcgggtta gtgcacttcg cgccgttcat aacgataaag
    10741 tctggtttga aacctgccag ctcttcatcg ctcgggcgaa taaacatgtt tttgacaaaa
    10801 tgcgcctgcc aggccacttc ggtgatgaaa cggacggaaa gacgagtatc cgggttcgca
    10861 ccacagaaag cgtcgacaac gaacagacgt ttgccggaaa gctgcctggt caccaggcct
    10921 ttcagatgct gccaggtttc cggagagaga ggtttgttgt cgttcttacc tttgcctttg
    10981 tctgcccacc agaaagtatc gcgagtggta tcgtcacgga cgatatactt atcttttggt
    11041 gaacgaccgg tgaagatccc ggtatcgacg gcaacggcac ccagattagt taacaccccg
    11101 cgctcataac ctgtcaggct cggatcgagc tcttcctgat acagcaggtc gtagcttggg
    11161 ttgtaaacga tatcatgtac gtcactgata ccataagcct cgagttcttg cgggggcaaa
    11221 ccattgttaa cgcgcatttc actgctcctt agccaatatg tattgcctga atagtaaagt
    11281 ctttttgggg gtgttaaccg cgacaaggct catagattta cgtatctgga gaaattcata
    11341 tttgacaaaa aactccgtga ttcctgtcac gaaacggttg ctattatcgc agaaatagca
    11401 ttttaggtgt ggaaaatgtt ctcaaaaggt taaatcttgg tttatatggc gggaatattg
    11461 tgagtggaat cgcattcctg aatgaaattg atgcaaattc aaccggtaaa gtatcgattc
    11521 accacgctaa ctttttttga ttattttctg aaaaaagcaa aatactactg cccggatgcg
    11581 ataattaacc caccttttga ttctggataa ccaacatgga taacgttgaa ctttcaccgg
    11641 caacacgctg ggggatgatt gctaccggat tacttcaggg gctggtttgc tacctgctta
    11701 tcgcctggct tgccggaaaa aatcacagtt ggattgttta tggcgtgcct gcgaccgtcg
    11761 cgttttcatc cgttttactc ttctccgtga tctcttttaa acagaagcgc ctttggggat
    11821 ggctggcgct ggtgtttatt gccacgacag ggatgagcgg ttggctgaag tggcaaactg
    11881 atggcatgaa cccctggaga gctgaaaagg cgatttggga ttttggctgc tatctgctgt
    11941 tgatggcaat gttgttgcta ccgtggatac aacaaagcct tcgtatccgc aatggcagta
    12001 gccgctatag ctacttttac cagtcagtat ggcataacgt acttatatta ctggtgattt
    12061 ttctctccaa tggcctgacg tggctggtgc ttttactgtg gggtgagttg tttaaacttg
    12121 ttggcatcac gtttttcaat acgctctttt ttgcaaccga ctggtttatg tatcttacat
    12181 ttggtctggt taccgcactg gcggtgatcc tcgcgcgtac acagtcacgt ttaatcgact
    12241 ctattcaaaa gttgttcacg ctaatcgcca cggggttgct gccgttagta tcattgctaa
    12301 ccccgatgtt tatcatcacc ctgccgttta cgggcctgag cgcgatttct cgccacatct
    12361 ccgccgccgg gttgctgtta acgctggcct ttttgcaatt gatcttaatg gctattgtcc
    12421 gcgatccgca aaaagcgtca cttccctgga cggggccgtt gcgttgcctg attaaaaccg
    12481 ctttgctggt tgctccgctg tatgtgttcg tcgccacctg ggggttatgg ctgcgggtcg
    12541 ctcagtacgg ctggactgtc gaccgcttgc agggcgcgct ggcggagctg gtgttactgg
    12601 tgtggtcgct ggggtatttt gtcagtatcg tctggcgtaa tgggcaaaat ccccttgttc
    12661 ttcagggcaa agtgaacctt gcggtttcgt tattggtgtt ggtgatactg gtgcttctta
    12721 attcgccggt gctggacagt atgcgtatta gcgtgaacag tcatatggcg cgttatcaga
    12781 gcggcaaaaa cacgccagac caggtaacta tctacatgct cgaacagagc ggtcgctatg
    12841 gacgtgcggc gcttgagtcg ctgaaaagcg atgccgggtt tatgaaagac ccgaaacgcg
    12901 cgcgggatct gctgatggcg ttagatggag agcaacatct tcaggaacag gtatcggaaa
    12961 aagtattcgc cgaaaatgtg ttaattgccc ctggttctgt taaaccggat gcgacattct
    13021 ggtcggcctt aatccaggat cgctataacg tgatgacctg tattgaaaaa gacgcctgcg
    13081 tactggtcga gcaagatctg aatagtgatg gtcaggcgga gcggatcctg tttgctttta
    13141 atgatgacag agtcattgtc tatggctttg actcagacag aaaagaatgg gacgcgcttg
    13201 atatgagttt acttccgaac gaaataacga aagaaaaatt actcacagct gcgaaggatg
    13261 gaaaactggg gacgaggccg aaagcgtggc gcgatctgac ggtagatggt gaaacgctgg
    13321 aaataaactt aagtaagtga caacgctttt atcggatacg acgctgtatc tgaccaaaat
    13381 aatttgcgat ttatttcata ggcgctggta agcgcagcac atcaggccat taagtcgtaa
    13441 aatccggcag agccctctct gccggacata ctcattaatg aacttacgga tctgccggag
    13501 acgcgttgtt gcggatttca gcaatatcca tcgcattgaa cagatagtgg ttaccgcagt
    13561 aatcacaatg catgtcaatt tcgccatctt ccgccaggat gctatcaact tcttcatcag
    13621 gcagcgtttt cagcgcatcg gcgcaacgtt cacgcgagca ggtgcatttg aactccacat
    13681 cctgcggatc gtaaaccgtc acctcttctt cgtgatacaa acgccacaac acttcgtttg
    13741 ccggtaaggt cagcagttct tcggttttga tggtttcggt tagcgtcgcc aggtggtcaa
    13801 agtcgtcctg ttgggtattt tgcgcaggca ttacctgcaa caacataccg cctgcagccg
    13861 gtttgccgtc tacgtcgccg gtgcgaataa acaggcgcgt cggcagctgt tcagaacgca
    13921 taaagtaatc ttctaggcag gccgccaggg tatcaccttc cagaccaact acgccctgat
    13981 agcgttcgcc ttcgctcggg gtaatggtga tcaccacgta accattgccg accagcgttt
    14041 tcaggtcggc attttctgga atttcgccct gcacgcgcgc cacaccgcgc atttgctggt
    14101 tattgttacc gttaataacc gccagattca tcggaccgtc gccctgcagc tgtacggtga
    14161 tatcaccatc aaacttcagc gtagcggtta acaggctggt cgcaaccagc agttctgcca
    14221 gcacgttttt aacgggctgc ggataatcgt ggttctcaag gatctgttgc agggtttccg
    14281 aaacggttac cagttcgccg cgaacggcaa aattttcaaa cagatagcga tgtaattggt
    14341 catgttgcgg cataatcatc tctcttgcag gtgacagtta ttcactgtcg ccgtgtttaa
    14401 atcgtaacag gtcgcggcgc tcttttttgt ccgggcgtcg gtccgggtgc ggcatggtta
    14461 aggcattaag tttacgtgcc atcgccattt tttcgcgttt ctctacactt tccgcagtct
    14521 cttcatacag caaggctgcc tcgctggcgg ggcgacgctg ttcagtaatc gcctttacaa
    14581 tcaccgtgcg ttcgtcattt ccctggcgca gagtgagcgt ggcattcagc tcgacgattt
    14641 tgctcggctt gctgcgctgc ccgttgtaat gcaccttacc gccttcaatc atttcacggg
    14701 ccagcgcgcg ggttttataa aaacgggcag cccatagcca tttatccagt cgaacctcaa
    14761 cagcaggttt ctctttcatg gcgtctcctt cacattagcg aggggatcag gcggcggtag
    14821 tcattcagtg acggatggcg ttgatactgt ttctcggcaa tcccggaatc aggattagtc
    14881 acgccgaggc agtaacgaat accaaattgc gcggcagcat cgagaatcgc ttcgctgtca
    14941 tcaataaaca gcgttctttc agctttcaga cccgtagctt cggccaccgc atgccataac
    15001 cgctgatcct ctttcggata accaaatgtg tgggtggaaa gtaataaatc aaggtgtgcg
    15061 tccagacccg tatgctcaag ttttaccgcc aggttgtgcg gatgagcatt ggtgagcaaa
    15121 attcgctgct taccgctggc tttcagtgcc tcaagaaacg gaatggtatc ttcacgcagt
    15181 acggcgcgcg gtcccatctc ggtggtcatc gcacagatat ccagacccag ttgctcactc
    15241 cagtaatcaa gacagtacca gtttagcgta tgctgtacgt cgtgatattg ctggcgcata
    15301 tattccatcg cttcctgtgg cgtaaccccg tttttcgcgc cccatgtttc aggcaccagc
    15361 ttttgccaga aatagttatc gaaggcgagg tcgagcaacg tgccgtccat atccagcaga
    15421 acggtatcta cgtcctgcca ggcaatgttg atatgcatga gggaaatctc cagagtgaag
    15481 caatttgcgc gacagggtag cataacctgc cgcgcaaacg tgttattcga taaggctttc
    15541 tgaaggggtg atcagttgcg ggttcaggca gctttcataa taggcctgaa tctccatcat
    15601 gcgagtgcga tgacgctggt aacgacgcca ggcctgtacg ccattgtaaa tggcgctacc
    15661 cagcatcgtc agtagcagca atgtggtgct gagatagcgc cacaggccgg aacgatccgg
    15721 gatcggatga aggccaatat gctgcgtacc attggcgtca gtgaagattt ttgtgacgat
    15781 accttcggcg ttaaacggcg tatgcatcag catttgtgcc agtttctgga aagcgttcca
    15841 ctgttcttgc ggcgggtagt cgtaaagtga tgccgaaggc cagggctgat caacaaaatc
    15901 gctgccttcg tcgctgacaa tcaggaatcc gccgggggcc ggactgttta gtgattgtgc
    15961 cgctcgcgcc gtttcatgcg tgataaacgg cgcggtggag gttgccacca ggttatccag
    16021 cgattccgca ctcaccgggc gtaataacac attcacgcca tcgagcttcc cggcgttggc
    16081 gcgttttacc agcgcgtccc agtctttact gttgccgaga ttgaccagcg catttttcag
    16141 tcgcacacag tcatctttgg cagaacataa atccgctgtc ttcagtacaa tgtcgccaaa
    16201 atcatcaagc aataccatgc cggatttttg aatcgctgaa cgcaatgagg cactgacgcg
    16261 agattcatct tccggtttag ggtgcagctg gcgattaact gcttcagtca atgccgtcgc
    16321 tttgttgacc agttcagatt ctggtaatgg taatgagcgg gcgtcattcc agatgatctg
    16381 cgagcagtca aacggtaaaa aaggtgaatt ggttttcgcg ctccaggttc cggaagtgcg
    16441 aatattacac attcccgtac cgctaatacg caatgtatcg cctacccgca cgccagcgtc
    16501 agccagttgt tttacgctgg tggcttcaat ggtctgcgcg cctttcatcc atgagagagt
    16561 gaatttcagc ggcatatcca gcgggatcca gaataacagc ataaacagca ccagcagcga
    16621 gcccgcagcg ataatcgtac tgcgcagcca gtgctgtaac ggaaagtttt ttacttcatc
    16681 atgcagcgaa agatatcgcc cctgacgcac tacgtggcgg tcgagataga tatcaatatc
    16741 ggtttgttga ccgagatcct gagcaatgta tggctgccag tgtgcgggat agaccaggtc
    16801 gataataccg agtgaaatat tgttgatctg ttcctgatcg ttttcgccaa acaatcccca
    16861 acgacggggt gtaccgcgca gacaatgaat ttctcgcagg gaggattttg ccgggggcgc
    16921 gaatagcccc cacagacctg cgcccagcag cagtaatgcg ccgcctgcca gccacggaac
    16981 aaatacatcc ggggtaatca ggcagaaaaa gaacatcagg aaggaggcga cgatcagcaa
    17041 cgcttcacgc agcccgcgcg gacgactcag cgcatattct tcatgcgttt ctttgcgaat
    17101 attgagtagc tcgatttgct cactctcttc cccgcgaatc gacgcctgcg tggacggaac
    17161 aggttgcaag gcatagccgc gcgtttcctg catgtactcc tgcagcgtat gaccgttgag
    17221 tgaaataacc aacggcagcg aatcggtatg aatcagttca acggtattct catcgttgat
    17281 gtactgttcc cagaaggggg gcaggtggac ttctaccgaa tcgaggtagt aacgccattt
    17341 attcggatcg tcggtagaga tgccgtaacg tgtgatagcg tgtgtcagca tcatgacgtt
    17401 gttgctttcg gcattcagcg ccagagagat cggtgccgcg ctagctcccg ttgggccagg
    17461 cacctgtaat acctgcgtca ggctctcaag ataattttca acggcgctac gttcttcagg
    17521 tgtgagttta cgcgtttgcg catccgcgaa ggcgttggtc cagggcagct gacgccgtct
    17581 ggatcgcact tttatcagcc atcccgcaag tagtgagcag gccagcaaag cagctaaaaa
    17641 aatcacaatg gtgctcatgc tttccccatc ttacttagtc tgtcaggcgt ggtcagtcgc
    17701 accggacaca atacatgatt ctgcggttgg ctatcggcaa gcgtggagct taacttgagc
    17761 atcattaagc acatcacagt aaccgctgat catagcaaac cctctgaagg cggaatatca
    17821 gcaaattcct ggagttattt caacctattg acttttaatt tgatttgagg acgattattt
    17881 attaagttac ataaatgtaa ataacatgca attcacattg ccgaaagcgt gtccgatatc
    17941 gcacaataac cgctgatttc acagcataga tcccacgatg aacaaatcat tacaaaaacc
    18001 caccattctg aatgttgaaa ctgtagcccg ttcccgactg tttaccgtcg agagcgtgga
    18061 tctggagttc agcaatggcg tgcggcgtgt ttatgaacga atgcgtccaa ccaaccggga
    18121 agcagtgatg attgtgccga ttgtggatga tcacctgatc ctgatccgcg aatacgcagt
    18181 gggaactgaa tcctacgaat taggtttttc gaaaggatta attgatccgg gtgaaagcgt
    18241 ctacgaagcc gctaaccgcg agctaaaaga agaggttgga tttggtgcga acgatctgac
    18301 ttttttgaag aagctcagca tggcaccgtc ttacttttcc agcaaaatga atatcgtggt
    18361 cgcgcaagat ctctacccgg aatcgctgga aggcgatgag ccagagccgc taccacaggt
    18421 gcgctggccg ctggcgcata tgatggattt gctggaagac cctgacttca acgaagcgcg
    18481 taatgtcagc gcgctgttcc tcgtgcgcga atggttgaaa gggcaggggc gagtgtaaat
    18541 gcgtgaagtt tgcctgatgc acaacgctta tcaggcttat ggggttcctg caatatattg
    18601 atttttcacg attttgttag ccggataacg cgttcacccg catccggcat aaacaaatgc
    18661 actttgtcag ccaactgaaa aggcgccgaa gcgccttttt aatcagaaca attcctgtgc
    18721 ctcaccatta tcgataatgg tcgttcccac ctcgtgcacc gcctgttgtg tcggctgcgt
    18781 accttcgatg aaatactctt cgcggctgtt gccaccatta gctaactgcc cggtgctgcg
    18841 atcgatattc accgtcacaa tacccggagg cggcgtcagc ggctgctccg gcacaccttc
    18901 aagaacggct ttcatataag cgtcccatgc aggctgggca ctcttggcac cgccttcgta
    18961 acctgagatc tgatctttaa tcgctccgga agccgttgta tgaccgagat tacgacggtg
    19021 atcatcaaag ccaatccaga ccgaggtcac aacgcccgga ccgtaacccg agaaccacgc
    19081 atctttcgaa ctgttagtgg taccggtttt cccgccgata tcgcgacgct gcaaatcacg
    19141 acctgcacgc cagccggtac cctgccagcc tggttcacca aagatattgg tgttcaaagc
    19201 actcttaatc aggaatgcca gcggagtgtt gatgacgtgc ggtgcgtact cctgcgcgcc
    19261 agtcttcgcc actaacgcct gatttgcctg ctccagctgc ggcattggta cagaaacatt
    19321 ctgctgctcg cgggagatag cgacatcttc aacatcgtta ttttccagca cgttcgattt
    19381 ctgcgtatca ccgtaaatca ctggaatatc gcattccggg caggctactt tcggtttcgc
    19441 ttcgaaaatc acgccgccct gatcgttttc aattttgctg ataaaccacg ggtccactag
    19501 gaagccgccg ttcgccatga ccgcgtagcc gcgcgccacc tgcattgggg tgaaggacgc
    19561 tgaacccagc gccagcgatt cggtgtggac aatgttttgc gccgggaagc cgaagcgttg
    19621 cagatattct gcagcgtagt cgacgcccat cgcccgcatt gcacgtacca tcaccacgtt
    19681 tttcgactga cccagcccct gacgtaagcg aattggacca gcatactgcg gtggtgagtt
    19741 cttcggctgc cagtcagaac cggcacctgc atcccagcga gaaattggca catcgttcaa
    19801 catacttgcc agcgtcagac ctttatccat cgccgcggtg tagaggaacg gtttgatgtt
    19861 ggaacccacc tgacgcagtg cctgggtggc gcggttaaac ttgctctgat tgaaatcaaa
    19921 gccaccgacc agcgccataa cggcaccatt ttgcggattg atcgacacca gcgccgagtt
    19981 cacttccggc acttgtgcca gccaccatgc atcgccaacc tgacgaaccc agatttgctg
    20041 acccgtttgc agaacatcgg tcactttacg cggcgtcggt ccctgctgag tatccgaacg
    20101 gtaaggacgc gcccagcgaa cgccttccat actcaatgcg acggtcgacc cgtccgccag
    20161 catcgccgtc gctccctgag gattggcgct ggtgactgcg gcaggcagca gcggaccata
    20221 ggtcggcagc gccttcagcg tatcggtaat cttgttgtta tcccacgccg actcgcccac
    20281 tttccacagc acatttgccg ggccgcgata gccgtggcgc atgtcgtagt ccagcacgtt
    20341 attacgtacc gcctgctgcg cggcctgctg cactttgcgg gtgatggtgg tgtaaatgcg
    20401 ataaccgtct tcataggcac tttcgccata acggttatac atctcctggc gcaccatttc
    20461 gctcaggtac ggcgcagaga aagcaatctc cggcgcgtga taattagcgt taatcgcctc
    20521 agtgcgtgtc tgatcgaact gttgctgggt gatataccct tcatccagca tccgcgacag
    20581 tacgacgtta cgccgcgcga cggcacgatc cattgagtag agcggattaa aggtggaagg
    20641 cgctttcggc aacccggcta tcaccgccat ttcgttcagc gtcagttggt cgaccgtttt
    20701 accgaaatag acttgtgccg cagcaccgac accataggcg cggtaaccaa ggtaaatctt
    20761 gttcagataa agctcgagga tttcgtcttt cgtcagcaac tgttcaatgc gaatcgcgag
    20821 gaagacttcc ttaatcttac gcatcatcgt gcgttctgga ctgaggaaga aatttctcgc
    20881 cagctgctgg gtaatggtac tcgccccttg tgacgcgtgg ccggagaaca gcgccacact
    20941 tgccgcacgg aagatcccca ccgggtcaac tccgtgatgc tcgtagaagc ggctgtcttc
    21001 cgtcgcgata aaggctttca ccatctccgg tgggatttga tccaacgtaa ccggaatacg
    21061 acgtttctca ccgtattgag caatcagctc gccatcggcg ctgtaaatct gcatcggaat
    21121 ttgcaggcga acatctttta atgtcaccac atccggcagt tgtggctcga tgtagcggta
    21181 taggccataa atcgagcctg ctcccagcag aatgcaacag actgcaagga tcaaaaaata
    21241 ctttacgaac ttcactggaa atttcccatt tagtttcatt tgggcagttt ataaacaaac
    21301 gcgcggtagt ataaaggcaa gccagacgca ttgatatacc cgtcagagtg acgggtgata
    21361 aggagatcat cacaatgaca tttaagatct ggcaaattgg tttgcattta caacagcaag
    21421 aagcggcagc ggttgcgatc gtgcggggcg caaaagaatg ctttttgcaa cgctggtggc
    21481 ggttgccgct ggcgcacgac attatcaaag atgggcggat tgttgatgcg cagcagctgg
    21541 ctaaaacgtt gttaccgtgg agtcgcgaac tgccgcagcg tcatcacatt atgttggcgt
    21601 ttcccgccag tcgcacatta cagcggtcat ttccgcgccc gtcgatgtcc cttggtgagc
    21661 gggagcaaac ggcctggctg tcagggacga tggcccgcga gctggatatg gatccggact
    21721 ccctgcgctt cgattacagc gaagactcac tcagccccgc ttataacgtg actgccgcgc
    21781 aaagcaaaga gctggcaacg ctgcttacgc tggcagaaag gttgcgtgtt catgttagtg
    21841 cgatcacccc ggatgccagt gcattacagc gattcctgcc ttttttacct tctcatcagc
    21901 aatgtctggc ctggcgtgat aacgaacagt ggctgtgggc gacgcgctat agctgggggc
    21961 gcaaactggc ggtggggatg actagcgcga aggagctggc ggcagcgtta tccgttgatc
    22021 ccgacagcgt cgcgatatgt ggcgaaggcg gatttgaccc ctgggaggcc gtttctgttc
    22081 gtcagccgcc gctaccgccg cctggtggag actttgccat cgcgctgggg ctggcgcttg
    22141 ggaaggcgta ctgatgaacc cgccaattaa ttttttgccc tggcgacagc aacgccggac
    22201 cgcttttctg cgtttctggt tgctgatgtt cgttgcgcct ctgctgctgg ccgtcgggat
    22261 aacgctaata ctgcgtctga caggcagcgc cgaagcgcgc atagacgcag ttttgcttca
    22321 ggcggaacaa caactcgccc gcagcttaca gataacgaag ccacgtttgc tggagcaaca
    22381 gcagttacgc gaacagcgtt cgcaacggca gcgccagcga caatttaccc gcgactggca
    22441 atctgcgctg gaagcactgg cggcgctttt acccgagcac gcctggctga caacgataag
    22501 ctggcagcag ggaacgctgg agatcaaggg gctgacaaca agcattaccg cgttaaacgc
    22561 actagaaacg tcactccgcc aggatgcgtc ttttcatctc aatcagcggg gagccacgca
    22621 gcaggatgcg cagggacgct ggcaatttga gtatcagtta acaaggaagg ttagcgatga
    22681 acatgttctt tgactggtgg ttcgccacat caccccgcct ccgccagttt tgctgggcat
    22741 tctggttgct gatgttagtt acgctcattt ttctatcatc gacacaccat gaagagcgcg
    22801 acgcattaat tcgactacgg gcaagtcatc accagcagtg ggccgcactg tatcgcctgg
    22861 tagacaccac tcccttcagc gaggaaaaaa cgctgccctt ttcgccactg gattttcagt
    22921 tatccggcgc gcaactggtt tcctggcatc catccgcgca gggaggcgag ttggcgttga
    22981 aaacgctgtg ggaagcagtg ccgtcggcat ttacacggct ggcagagcgc aacgtcagcg
    23041 tgagccgttt ttcgttaagc gtggaaggtg atgatctttt gttcacgcta caactggaga
    23101 tgccgcatga gggttaaacg ctggttgttg gcaggtattg cattgtgcct tttaaccggt
    23161 atgcgtgacc cttttaaatc gccggaagat ctatgccgga ttagcgaact tagccagtgg
    23221 cgctatcagg ggatggtagg gcgaggcgag cgcatcatcg gtgtaataaa agacgggcaa
    23281 aagaaatggc gacgggtgca gcaaaacgat gtgctggaaa acggctggac aattttacag
    23341 ctgacgccag acgtactaac gctgggtacc gggacaaact gcgaaccgcc acaatggttg
    23401 tggcaacggc aaggagatac aaatgaagca atggatagcc gcactactgt tgatgctgat
    23461 acccggcgta caggcggcaa agccgcaaaa agtgacgctg atggtggatg acgttccggt
    23521 agctcaggtg ttgcaggcgc tggctgaaca ggagaagttg aacctggtcg tgtcgccaga
    23581 cgtcagcggt acggtgtcgt tacatctaac agatgttccc tggaagcagg cactacaaac
    23641 tgtagtgaaa agcgccggac tgataacgcg gcaggaaggc aacattctct cagtgcattc
    23701 cattgcctgg cagaatgaca atatcgcccg ccaggaggcg gagcaggcgc gggcgcaggc
    23761 aaatctgccg ctggaaaatc gcagtataac cctgcaatac gccgacgcgg gagaactggc
    23821 gaaagcgggg gagaagctac tgagtgccaa agggagtatg accgtcgata aacgcaccaa
    23881 tcgccttttg ctacgagata acaaaacggc gttaagcgcg cttgaacagt gggtagcgca
    23941 aatggatctg ccggtcgggc aggttgagct gtcggcgcat attgtcacca ttaatgaaaa
    24001 aagtttgcgt gagttaggcg tgaaatggac gctggccgat gcgcaacacg ctggtggcgt
    24061 tgggcaagtc accacgcttg gtagcgacct ctccgtagcg acggcgacaa cgcatgtcgg
    24121 ttttaacatt gggcgcatca acggacgctt gctggatctt gagctttccg cgctcgaaca
    24181 aaaacagcag ctggatatta tcgccagtcc gcgtctgctg gcctcacatc ttcagcctgc
    24241 cagcattaaa caggagagcg aaattccata tcaggtttcc agcggggaaa gtggcgcgac
    24301 gtcggtggaa tttaaagagg ccgtcctggg gatggaggtc acgcccacgg tgttacaaaa
    24361 aggtcgcatc cggctgaaat tacacatcag ccagaacgtt ccggggcagg tgctacagca
    24421 ggccgatggc gaagtgctgg cgattgataa gcaggagatc gaaacgcagg tcgaggtaaa
    24481 aagcggagaa acgttggcgc tgggcggcat ttttacccgt aaaaataaat cgggtcagga
    24541 tagcgtaccg ttgcttggcg acattccctg gttcgggcaa ttatttcgtc atgacggaaa
    24601 agaagatgaa cgacgcgagt tagtggtgtt tatcacgcca cgactggttt ccagtgagta
    24661 aacagccgta aaagcggtaa tgtttttatg ctgaacgtgt ttcatctatt tgacgcgcgc
    24721 aggtatttag catacaagga gtaccgattt gagagttggt gctcttcgct gcctgcgttc
    24781 catgatgatg atttatcatt caggcggcat tttgctgtct tttttacgct aatcttaccc
    24841 ggtgatttat cgccagagcg gtggtagcaa ggcagcgcgc ttgcagcgac cagatatgca
    24901 gagggatggg tgatttattc agttgccaaa cccgctggag tattgagata attttcagtc
    24961 tgactctcgc aatatcttat gaggtttcag ttcatgtcct gcggcgctct ctgagcgaag
    25021 cgggtttatc attaacgaat agtcttagta gtaccgaaaa aatggcagag aaacgcaata
    25081 tctttctggt tgggcctatg ggtgccggaa aaagcactat tgggcgccag ttagctcaac
    25141 agctcaatat ggaattttac gattccgatc aagagattga gaaacgaacc ggagctgatg
    25201 tgggctgggt tttcgattta gaaggcgaag aaggcttccg cgatcgcgaa gaaaaggtca
    25261 tcaatgagtt gaccgagaaa cagggtattg tgctggctac tggcggcggc tctgtgaaat
    25321 cccgtgaaac gcgtaaccgt ctttccgctc gtggcgttgt cgtttatctt gaaacgacca
    25381 tcgaaaagca acttgcacgc acgcagcgtg ataaaaaacg cccgttgctg cacgttgaaa
    25441 caccgccgcg tgaagttctg gaagcgttgg ccaatgaacg caatccgctg tatgaagaga
    25501 ttgccgacgt gaccattcgt actgatgatc aaagcgctaa agtggttgca aaccagatta
    25561 ttcacatgct ggaaagcaac taattctggc tttatataca ctcgtctgcg ggtacagtaa
    25621 ttaaggtgga tgtcgcgtta tggagaggat tgtcgttact ctcggggaac gtagttaccc
    25681 aattaccatc gcatctggtt tgtttaatga accagcttca ttcttaccgc tgaaatcggg
    25741 cgagcaggtc atgttggtca ccagcgaaac cctggctcct ctgtatctcg ataaggtccg
    25801 cggcgtactt gaacaggcgg gtgttaacgt cgatagcgtt atcctccctg acggcgagca
    25861 gtataaaagc ctagctgtac tcgataccgt ctttacggcg ttgttacaaa agccgcatgg
    25921 tcgcgatact acgctggtgg cgcttggcgg cggcgtagtg ggcgatctga ccggcttcgc
    25981 ggcggcgagt tatcagcgcg gtgttcgttt cattcaagtc ccgacgacgt tactgtcgca
    26041 ggtcgattcc tccgttggcg gcaaaactgc ggtcaaccat cccctcggta aaaacatgat
    26101 tggcgcgttc taccagcctg cttcagtggt ggtggatctc gactgtctga aaacgcttcc
    26161 cccgcgtgag ttagcgtcgg ggctggcaga agtcatcaaa tacggcatta ttcttgacgg
    26221 tgcgtttttt aactggctgg aagagaatct ggatgcgttg ttgcgtctgg acggtccggc
    26281 aatggcgtac tgtattcgcc gttgttgtga actgaaggca gaagttgtcg ccgccgacga
    26341 gcgcgaaacc gggttacgtg ctttactgaa tctgggacac acctttggtc atgccattga
    26401 agctgaaatg gggtatggca attggttaca tggtgaagcg gtcgctgcgg gtatggtgat
    26461 ggcggcgcgg acgtcggaac gtctcgggca gtttagttct gccgaaacgc agcgtattat
    26521 aaccctgctc acgcgggctg ggttaccggt caatgggccg cgcgaaatgt ccgcgcaggc
    26581 gtatttaccg catatgctgc gtgacaagaa agtccttgcg ggagagatgc gcttaattct
    26641 tccgttggca attggtaaga gtgaagttcg cagcggcgtt tcgcacgagc ttgttcttaa
    26701 cgccattgcc gattgtcaat cagcgtaaca acaagaaagg tcaggccgct tatcaagcgg
    26761 tctattagct tcaggttaat tgcaacgtgg taagcattaa ccttttagtg gggtgttaaa
    26821 tggatgaatt caaaccagaa gacgagctga aacccgatcc cagcgatcgt cgtactggtc
    26881 gttctcgtca atcttctgaa cgttctgagc gtactgaacg tggcgaaccg cagatcaatt
    26941 ttgatgatat tgaacttgat gacactgacg atcgccgtcc gactcgtgcg caaaaagagc
    27001 gcaatgagga accggaaatc gaagaagaaa ttgacgaatc cgaagatgaa accgtggatg
    27061 aagagcgcgt agagcgtcgt ccgcgtaagc gcaaaaaagc agccagtaaa cccgcttctc
    27121 gtcagtatat gatgatgggc gtcggcattc tggttctact gctgttgatc atcggtatcg
    27181 gttctgcgct aaaagccccc tcgaccactt ccagcgatca aaccgcgtct ggcgagaaga
    27241 gtattgatct tgctggcaat gcgaccgatc aggcgaatgg cgtgcagcca gcgccgggaa
    27301 ccacgtctgc ggaaaatact cagcaggatg tttcgctgcc accgatctct tctacgccga
    27361 ctcaagggca aaccccggtg gcaacggatg gtcaacaacg tgttgaagtg cagggtgacc
    27421 tgaacaatgc gctgacccag ccacaaaatc agcaacagct gaacaatgtg gcggtcaatt
    27481 ccacgttgcc gactgaaccc gcaacggttg cgcctgttcg caatggcaat gcatcgcgtg
    27541 actcggcgaa aacgcaaacc gctgaacgtc cgtccactac gcgcccagtt cgtcagcagg
    27601 cggtgattga accgaaaaaa ccgcaagcaa ccgtgaaaac ggagccgaag ccggtagcac
    27661 agacgccgaa gcgtactgaa ccagctgccc ctgtggcgag cacgaaggca ccggctgcga
    27721 cttctacgcc agcaccaaaa gagacggcga ctacggctcc agtacagacg gcatccccgg
    27781 cgcaaaccac ggcaacacca gccgctggag ggaagaccgc aggtaatgtt ggttcgttga
    27841 aatcggcacc gtccagccat tacactctgc agctgagcag ttcctctaac tacgacaacc
    27901 tgaacggttg ggcgaagaaa gagaatctga aaaactacgt tgtctatgaa acgacgcgta
    27961 atggtcagcc gtggtatgtc ctggtttctg gcgtgtacgc ttcgaaagaa gaggcgaaaa
    28021 aagcggtatc tacattgcca gcagatgttc aggccaaaaa cccgtgggcg aaaccgctgc
    28081 gtcaggtaca ggccgatctg aagtaatcaa ggttatctcc cgcaatggtt tatcgttgcg
    28141 ggagctgcct gaagcgctgg atgctgtcgg agctttctcc acagccggag aaggtgtaat
    28201 tagttaatcg gcatgaagaa aaatcgcgct tttttgaagt gggcaggggg caagtatccc
    28261 ctgcttgatg atattaaacg gcatttgccc aagggcgaat gtctggttga gccttttgta
    28321 ggtgccgggt cggtgtttct caacaccgac ttttctcgtt atatccttgc cgatatcaat
    28381 agcgacctga tcagtctcta taacattgtg aagatgcgta ctgatgagta cgtacaggcc
    28441 gcacgcgagc tgtttgttcc cgaaacaaat tgcgccgagg tttactatca gttccgcgaa
    28501 gagttcaaca aaagccagga tccgttccgt cgggcggtac tgtttttata tttgaaccgc
    28561 tacggttaca acggcctgtg tcgttacaat ctgcgcggtg agtttaacgt gccgtttggc
    28621 cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag
    28681 aatgcctttt tctattgtga gtcttacgcc gatagcatgg agcgcgcaga tgatgcatcc
    28741 gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt tacggcgtat
    28801 cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc cgaaggtctg
    28861 gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg
    28921 tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc
    28981 acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg
    29041 aaaaaataat tctcaaggag aagcggatga aacagtattt gattgccccc tcaattctgt
    29101 cggctgattt tgcccgcctg ggtgaagata ccgcaaaagc cctggcagct ggcgctgatg
    29161 tcgtgcattt tgacgtcatg gataaccact atgttcccaa tctgacgatt gggccaatgg
    29221 tgctgaaatc cttgcgtaac tacggcatta ccgcccctat cgacgtacat ctgatggtga
    29281 aacccgtcga tcgcattgtg cctgatttcg ctgccgctgg tgccagcatc attacctttc
    29341 atccagaagc ctccgagcat gttgaccgca cgctgcaact gattaaagaa aatggctgta
    29401 aagcgggtct ggtatttaac ccggcgacac ctctgagcta tctggattac gtgatggata
    29461 agctggatgt gatcctgctg atgtccgtca accctggttt cggcggtcag tctttcattc
    29521 ctcaaacact ggataaactg cgcgaagtac gtcgccgtat cgacgagtct ggctttgaca
    29581 ttcgtctgga agtggacggt ggcgtgaagg tgaacaacat tggcgaaatc gctgcggcgg
    29641 gcgcggatat gttcgtcgcc ggttcggcaa tcttcgacca gccagactat aaaaaagtca
    29701 ttgatgaaat gcgcagtgaa ctggcaaagg taagtcatga ataagtttga agatattcgc
    29761 ggcgtcgctt ttgatcttga tggtacgctg gtcgacagtg ctcctggtct tgctgctgcg
    29821 gtagatatgg cgctgtatgc gctggagttg cccgtcgcag gtgaagaacg cgttattacc
    29881 tggattggta acggcgcaga tgttctgatg gagcgcgcat tgacctgggc gcgtcaggaa
    29941 cgtgcgactc tgcgtaaaac aatggggaaa ccgcccgttg atgacgacat tccggcagaa
    30001 gaacaggtac gtattctgcg taaactgttc gatcgctact atggcgaggt tgccgaagag
    30061 gggacgtttt tgttcccgca cgttgccgat acgttgggcg cgttgcaggc taaaggcctg
    30121 ccgctaggct tggtcaccaa caaaccgacg ccgttcgtcg cgccgctgct cgaagcctta
    30181 gatattgcca aatactttag cgtggttatc ggcggcgatg atgtgcaaaa caaaaaaccg
    30241 catccggacc cgctgttact ggtggctgag cggatgggaa ttgccccaca acagatgctg
    30301 tttgtcggcg actcacggaa tgatattcag gcggcaaaag cggcaggttg cccatcagtt
    30361 ggcttaacct acggatacaa ctacggcgag gctatcgatc tcagccagcc tgatgtaatt
    30421 tatcagtcta taaatgacct tctgcccgca ttagggcttc cgcatagcga aaatcaggaa
    30481 tcgaaaaatg actaagccca tcgtttttag tggcgcacag ccctcaggtg aattgaccat
    30541 tggtaactac atgggtgcgc tgcgtcagtg ggtaaacatg caggatgact accattgcgt
    30601 ttactgtatc gttgaccaac acgcgatcac cgtgcgccag gatgcacaga agctgcgtaa
    30661 agcgacgttg gatacgctgg ccttgtatct ggcttgtggt atcgatcctg agaaaagcac
    30721 catttttgtt cagtcccacg taccagaaca tgcgcagtta ggctgggcac tgaactgcta
    30781 tacctacttc ggcgaactga gtcgcatgac gcagtttaaa gataaatctg cgcgttatgc
    30841 cgagaacatc aacgctggtc tgtttggcta tccggtgctg atggctgcgg acatcctgct
    30901 gtatcaaact aatctggtac cggtgggtga agaccagaaa cagcacctgg aactgagtcg
    30961 cgatatcgcc cagcgtttca acgcgctgta tggcgagatc tttaaggtgc cggagccgtt
    31021 tattccgaaa tctggcgcgc gcgtaatgtc gctgctggag ccgaccaaga agatgtccaa
    31081 gtctgacgat aaccgcaata acgttatcgg cctgctggaa gatccgaaat cggtagtgaa
    31141 gaaaatcaaa cgcgcggtca ctgactccga cgagccgccg gtagttcgct acgatgtgca
    31201 gaacaaagcg ggcgtttcca acctgctgga tatcctttct gcggtaacgg gccagagcat
    31261 cccagaactg gaaaaacagt tcgaaggcaa gatgtatggt catctgaaag gtgaagtggc
    31321 tgatgccgtt tccggtatgc tgactgaatt gcaggaacgc tatcaccgtt tccgcaacga
    31381 tgaagccttc ctgcaacagg tgatgaaaga cggcgcagaa aaagccagtg tacacgcttc
    31441 ccgtacgctg aaagcggtgt acgaagcgat tggttttgtg gcgaagccgt aatatcaaaa
    31501 cgctcggacg gtcccctcgc ccctttgggg agagggttag ggtgagggga gcaggcaagc
    31561 gccagtggga attacccttt gctcgcattg tcgaataaat cacttcgtca tcactctgaa
    31621 accggggaaa cccggttttt ttatcctctt ttcgcaacaa cccgaaaaaa tgtgaagcgc
    31681 ctcgccgttt ccacatctcg tcgtcgccac ttatcttcat tttaatgaag atataaatat
    31741 tcattttatt gaaaatatat tatgcgtcga acgtttatca aaaaagaagg cgtcgtcatc
    31801 acgacactgg cccgttattt gttgggtgaa aaatgcggta atcgactgaa aaccatagat
    31861 gagctggcaa ctgaatgccg ttcatcggtt ggcctgacgc aggccgcgtt gaaaacgctg
    31921 gaatcaagcg gagcgatacg gattgaacgc cgtgggcgca atggcagtta tcttgtcgag
    31981 atggataaca aagcattgct gagtcatgtg gatatcaaca acgtggtatg tgcgatgcct
    32041 ttgccctata cccgcttgta cgaaggttta gcgagcggat tgaaagccca gtttgatggc
    32101 attccttttt actatgcgca tatgcgcggc gcggatattc gcgtggagtg tttacttaac
    32161 ggtgtctatg acatggcagt agtttcccgc ctggcggcgg aaagttatct cagccaaaac
    32221 aatttgtgca tcgccctcga gcttggcccg catacgtatg ttggtgaaca tcaactgata
    32281 tgccgcaaag gtgagtctgg aaatgttaag cgagtaggtc tcgatagtcg ttcggcggat
    32341 cagaaaatca tgaccgatgt cttttttggc gatagtgatg tggaacgagt cgatctctct
    32401 tatcacgaga gtttacaacg cattgttaaa ggcgatgtcg atgcggtgat ctggaacgtg
    32461 gtggcggaaa acgaactgac catgctggga ttagaggcga cgccgctaac agacgatccg
    32521 cgatttttac aggccaccga agccgtggtc ctgacgcgag tcgatgatta cccaatgcaa
    32581 caattgctgc gtgctgttgt agataaacac gccctgcttg cccatcaaca acgggtggtg
    32641 agtggggaac aggaacccag ttattaaacg aaaggcatct gatatggaaa ccagactcaa
    32701 cctgctttgc gaggcaggcg ttattgataa ggacgtctgc aaaggcatga tgcaggtcgt
    32761 caacgtactg gaaaaagagt gccatctgcc ggtgcgcagt gagcaaggaa cgatggcgat
    32821 gacacatatg gcgagtgcac tgatgcgcag tcgccgtggt gaagaaatag agccgctgga
    32881 taacgagttg ctggcggaac tggcgcaatc cagccactgg caagccgttg tgcaattgca
    32941 tcaggagttg ttgaaggaat tcgctctgga agttaacccg tgtgaagaag gctattttct
    33001 ggccaatctt tatggattat ggatggctgc taacgaagag gtttgaagct cccgacaaat
    33061 ccacgtcgca caacctaaat attcaaaaaa atgaatattt aattcaagga acagaaacga
    33121 ggcaacaaga tgtttgtaga agcattgaaa cgccagaacc cggcgctgat ttccgccgca
    33181 ctaagtttgt ggcagcaggg caaaatcgcc ccggacagct gggtgatcga cgtggatcag
    33241 atactggaaa acggtaagcg gctcattgag acggcgcggc tttacggcat tgaactgtat
    33301 ctgatgacca agcaatttgg tcgtaatccg tggctggcgg aaaaattgct ggcattaggc
    33361 tacagcggca ttgtggcggt ggattacaaa gaggcgcgag tcatgcgccg cgctggtttg
    33421 ccagtggcgc atcaggggca tctggtacaa atcccttgtc atcaggttgc tgacgccgtt
    33481 gaacagggca ccgacatcat caccgtgttt actctcgaca aagcgcggga agtttctgcg
    33541 gcagcggtta aggccggacg ggtccagcct gtgctgctca aagtttatag cgacgatgat
    33601 tttctttatc cggggcagga gagcggtttt gtacagcatt cgctgcacga ggtgattgct
    33661 gaaattaaga aactgccggg gctgcattta gccggactaa cccatttccc ttgcctgctt
    33721 tgggatgagg ctgctgggaa agttttgcca acaccgaatc ttcacacgct ggtacaagca
    33781 cgggatcaac tggcgaaatc tggtattgcg attgagcaac tgaacgtacc ttcagcgacc
    33841 agctgcactt cgctgccatt gctggcggaa tacggtgcga ctcatgccga acccggtcat
    33901 gcgctgaccg gtactattcc ggcaaaccag cagggcgatc agcctgaacg tatcgcgatg
    33961 ctctggttaa gtgaaatctc ccatcatttc cgtggcgaca gctactgcta cggcggcggt
    34021 tactaccgtc gtggtcatgc gcaacatgcg ctggtgttta cgccagaaaa tcaaaagatt
    34081 actgaaacca atctcaaaac tgtggatgac agcagtatcg actacaccct gccgctggca
    34141 ggcgagtatc cggtgagcag tgcagtggtg ctctgttttc gcacgcagat ttttgtcacc
    34201 cgtagcgatg tggtgctggt gtccggtatt cagcgcggcg aaccagaaat agttggtcgt
    34261 tatgacagcc tcggcaaccc tctggaggca taaatggcgc gatttgtggt gttagtgatt
    34321 gatagctttg gcgtaggggc aatgaaagat gtcacgctgg tgcgcccgca agatgccggg
    34381 gcgaatacct gtggtcacat cctgagccag ttgccgcatt tgcagctacc aacgctggag
    34441 aagctggggc taatcaacac attgggttat gcgccaggcg atatgcagcc gtcagattcc
    34501 gcaacctggg gcgtggcaga gctgcaacat gaaggtggcg atacctttat ggggcatcag
    34561 gaaattttag gcacgcgccc gttaccgccg ctgcggatgc cttttcgcga tgtgattgac
    34621 cgtgttgagc aggcattagt ttccgctggc tggcaggtgg agcgccgtgg cgatgatctg
    34681 caatttctgt cggtcaatca ggcggttgcg attggcgata atctcgaggc ggatttaggc
    34741 caagtctata acattaccgc caatctctct gtgatctctt ttgacgacgc aatcaaaatt
    34801 ggtcgtatcg tgcgtgacca ggtacaggtc ggtcgggtca ttacatttgg cggcctgtta
    34861 accaacagtc agcgtattct cgatgccgca gaaagcaaag aagggcgctt tattggtatt
    34921 aatgcgccgc gttctggcgc ttatgacaac ggcttccagg tcgtgcatat gggctacggt
    34981 gttgatgaaa aagtgcaggt gccgcaaaaa ctgtatgaag cgggcgtgcc aaccgtgctg
    35041 gtgggtaagg tggcagatat cgtcagcaat ccttatggtg tgagctggca aaacctggtg
    35101 gatagccagc ggattatgga tatcaccctc gacgaattta acacccatcc gacggcgttt
    35161 atttgcatca atatccagga aaccgacctc gctggtcatg cagaagacgt cgcacgttat
    35221 gccgaacgtt tgcaggtcgt tgaccgtaac cttgcccggc ttgttgaggc gatgcagcca
    35281 gatgattgcc tggtcgtgat ggcggatcac ggtaacgatc cgaccattgg tcacagccac
    35341 catacccgtg aagtggtgcc agtgctggtt tatcagcaag ggctggtcgc cacgcagctc
    35401 ggtgtgcgca ccacgctttc tgatgtggga gctaccgtgt gtgaattttt ccgtgcgcca
    35461 ccgccacaaa atggtcgctc ttttctttcc tccctccggt ttgcaggaga caccctatga
    35521 gttttgatcc gacgggttac accctggccc atgagcatct gcatattgat ctctccggct
    35581 ttaaaaacaa cgtggactgc cgccttgatc agtatgcgtt catttgccag gagatgaacg
    35641 acctgatgac ccggggtgta cgtaatgtga ttgagatgac caaccgttac atggggcgca
    35701 atgcgcaatt tatgctcgat gtaatgcgcg aaaccgggat caacgtggtg gcctgtactg
    35761 gttattacca ggatgcgttt ttcccggaac atgtggtgac ccgcagcgtg caggaactgg
    35821 cgcaggagat ggtcgatgaa attgaacagg gtatcgatgg cacggagctg aaagccggga
    35881 tcatcgcgga gatcggctcc agcgaaggaa agattacgcc gctggaagag aaggtattta
    35941 ttgccgctgc gctggcgcat aaccagaccg gacgcccgat ctccacgcat acgtcgttca
    36001 gcaccatggg gctggaacaa ctggctctgt tgcaagccca cggagttgat ctttcgcgcg
    36061 tcaccgttgg tcactgcgat ctgaaagaca acctcgacaa cattttgaag atgatcgatc
    36121 tcggcgcgta cgtgcagttc gacaccattg gtaagaacag ttactacccg gacgaaaagc
    36181 gcattgcgat gctccacgcg ctacgtgacc gtgggttgct gaaccgcgtc atgctgtcga
    36241 tggatattac gcgccgctcc catttaaaag ccaatggtgg ttatggctat gactatttat
    36301 tgaccacctt tattccgcaa ttgcgccagt caggattcag tcaggccgat gtggatgtga
    36361 tgttacgtga aaatccctct caatttttcc aataaggaca gactcatgaa aaagattggc
    36421 gttgcaggct tacagcgtga gcagattaaa aaaaccattg aagcgacggc tcctggctgt
    36481 tttgaagttt tcattcacaa cgacatggaa gcggcaatga aggtgaaatc cgggcaacta
    36541 gattattaca ttggcgcgtg taataccggt gcgggcgcgg cattgtcgat tgccatcgcg
    36601 gtgattggct ataacaaaag ttgcaccatt gccaaaccag gcattaaagc gaaagacgag
    36661 catatcgcca aaatgatcgc tgaagggaaa gtggcgtttg gcctttccgt tgagcacgtc
    36721 gaacacgcga ttccgatgtt gattaaccat ctgaaataaa aggcacgact atggatctgt
    36781 atattcagat tatcgtggtg gcgtgcctga cgggtatgac atcgcttctg gcgcatcgct
    36841 cggcggctgt ttttcatgac ggcatccacc cgatcctgcc gcaactgatt gaaggctata
    36901 tgaaccgtcg cgaggcgggg agtatcgctt ttggtctgag cattggtttt gtggcctcgg
    36961 tggggatctc ttttaccctg aaaaccgggc tgctcaacgc atggttactc tttcttccta
    37021 ccgatatcct cggcgtactg gcgataaaca gcctgatggc gtttggtctt ggcgctatct
    37081 ggggcgtgtt gatccttact tgcctgttgc cagttaacca gctgctgacc gcgctgccgg
    37141 tggatgtatt aggtagcctc ggggaattaa gctcgccggt ggtttctgct tttgcactgt
    37201 tcccgctggt ggcgattttc taccagtttg gctggaagca aagtctggtc gccgccgtgg
    37261 ttgttctgat gacccgtgtg gtagtcgtgc gctatttccc acatcttaac cctgaatcca
    37321 tcgaaatctt tattggcatg gtgatgctgc tggggatcgc gataactcac gacctgcgtc
    37381 atcgtgatga aaatgacatc gatgccagcg ggctttcggt gtttgaagag cgcacgtcgc
    37441 ggattatcaa aaacttacct tatatcgcca tcgtgggagc attgattgcc gccgttgcca
    37501 gtatgaagat ttttgctggt agcgaagttt cgatcttcac actggagaaa gcgtattccg
    37561 caggcgtgac gccggaacaa tcgcaaacgc tgattaatca ggcggctctg gcagaattta
    37621 tgcgcggact ggggtttgtg ccgttgattg ccaccaccgc cttagcaacg ggtgtgtatg
    37681 cagttgcggg ctttaccttt gtttatgcgg tgggctatct ctcgccgaat ccgatggttg
    37741 cagcggtatt aggcgcagtg gttatttcgg cggaagtctt gctgcttcgt tcgatcggca
    37801 aatggctggg acgctacccg tcggtgcgta atgcgtcgga taacatccgt aacgccatga
    37861 atatgctgat ggaagtggcg ctgctggtcg gttcgatttt cgcagcaatt aagatggcgg
    37921 gttataccgg attctctatc gcggttgcca tttacttcct caacgaatcc ctgggccgtc
    37981 cggtacagaa aatggcggca ccggtcgtgg cagtaatgat caccggtatt ctgctgaatg
    38041 ttctttactg gcttggcctg ttcgttccgg cttaaggaag gcacctatga agacgtttcc
    38101 tttgcaaagc ctgactcttg ctgaagcgca gcaaaagcag tttgcgctgg tggacactat
    38161 ttgccgccat tttccaggca gcgagtttct tgctggcggt gatttagggt taactccaag
    38221 tctgaatcaa ccgcgtatta cccaacgggt ggagcaggtg ctggctgatg catttcacgc
    38281 acaggccgcg gcgctggtgc agggcgcggg gactggcgcg attcgcgccg ggctggcggc
    38341 tttgctcaaa ccggggcagc gtcttctggt gcatgacgcg cctgtttacc cgacgacacg
    38401 ggttattatt gagcagatgg ggctgacgct tattactgct gatttcaatg acctgtcggc
    38461 actgaagcag gtcgtcgacg agcaacaacc tgatgccgcg ctggtgcagc atacgcgcca
    38521 gcagccgcag gacagctatg tgctggcaga tgtgctggca acgttgcgcg cggcaggtgt
    38581 tccagcgtta accgatgata actatgcggt gatgaaggta gcgcgaatcg gctgtgaatg
    38641 cggcgcgaat gtctcgacat tctcctgctt caagctgttt gggccggggg gcgttggtgc
    38701 agtggttggc gatgccgatg ttattagtcg gattcgcgcc acgctttact ccggcggtag
    38761 ccagatccag ggctcacagg cgctggaagt cttgcgtggt ctggtgttcg cgccagtgat
    38821 gcatgcggtg caggcgggag tatccgaacg gttgctggct ttgcttaacg gcggcgcggt
    38881 ggcggaagtg aaaagcgccg tcattgccaa tgcgcagtcg aaggtgttga ttgtcgagtt
    38941 tcatcagctg attgccgcca gggtgctgga agaagcgcaa aagcgtggcg cgttgcctta
    39001 cccggtcggt gcagagtcga aatatgaaat cccgccgctc ttctatcgac tttcaggaac
    39061 gtttcgccag gcgaatccac aactcgaaca ttgtgcgatt cgcattaacc cgaatcgcag
    39121 cggtgaagag acggtgctgc ggattttgcg tgagagtatt gcgagtattt aatgctggat
    39181 agcaccgttc ggcaatcaag ctaaattccg cactgaacgg tcccctcgcc cc
//
LOCUS       DBKELA010000026        38253 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.026, whole genome shotgun sequence.
ACCESSION   DBKELA010000026 DBKELA010000000
VERSION     DBKELA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.026"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1222
                     /locus_tag="ACUQR0_001349"
     CDS             <1..1222
                     /locus_tag="ACUQR0_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HHV9108465.1"
                     /translation="ELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHP
                     VITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMII
                     PEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIA
                     LLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYV
                     NTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNF
                     KVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETV
                     LNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKY
                     LLAQFGVDIGEAAQ"
     gene            1219..2184
                     /gene="glpX"
                     /locus_tag="ACUQR0_001350"
     CDS             1219..2184
                     /gene="glpX"
                     /locus_tag="ACUQR0_001350"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HHV9108466.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2206..2715
                     /gene="fumE"
                     /locus_tag="ACUQR0_001351"
     CDS             2206..2715
                     /gene="fumE"
                     /locus_tag="ACUQR0_001351"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HHV9108467.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2712..3425
                     /locus_tag="ACUQR0_001352"
     CDS             2712..3425
                     /locus_tag="ACUQR0_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HHV9108468.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3397..4079)
                     /locus_tag="ACUQR0_001353"
                     /pseudo
     CDS             complement(3397..4079)
                     /locus_tag="ACUQR0_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(4073..4750)
                     /locus_tag="ACUQR0_001354"
     CDS             complement(4073..4750)
                     /locus_tag="ACUQR0_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9108469.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4738..5445)
                     /locus_tag="ACUQR0_001355"
                     /pseudo
     CDS             complement(4738..5445)
                     /locus_tag="ACUQR0_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5446..6024)
                     /locus_tag="ACUQR0_001356"
     CDS             complement(5446..6024)
                     /locus_tag="ACUQR0_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHV9108470.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6047..6478)
                     /locus_tag="ACUQR0_001357"
                     /pseudo
     CDS             complement(6047..6478)
                     /locus_tag="ACUQR0_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6850..7869
                     /gene="epd"
                     /locus_tag="ACUQR0_001358"
     CDS             6850..7869
                     /gene="epd"
                     /locus_tag="ACUQR0_001358"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HHV9108471.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7919..9082
                     /gene="pgk"
                     /locus_tag="ACUQR0_001359"
     CDS             7919..9082
                     /gene="pgk"
                     /locus_tag="ACUQR0_001359"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HHV9108472.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9297..10376
                     /gene="fbaA"
                     /locus_tag="ACUQR0_001360"
     CDS             9297..10376
                     /gene="fbaA"
                     /locus_tag="ACUQR0_001360"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HHV9108473.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10567..11427
                     /gene="mscS"
                     /locus_tag="ACUQR0_001361"
     CDS             10567..11427
                     /gene="mscS"
                     /locus_tag="ACUQR0_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HHV9108474.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11566..12201
                     /gene="argO"
                     /locus_tag="ACUQR0_001362"
     CDS             11566..12201
                     /gene="argO"
                     /locus_tag="ACUQR0_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HHV9108475.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12294..13034
                     /locus_tag="ACUQR0_001363"
     CDS             12294..13034
                     /locus_tag="ACUQR0_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HHV9108476.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13201..14151
                     /gene="srsR"
                     /locus_tag="ACUQR0_001364"
                     /gene_synonym="ygfI"
     CDS             13201..14151
                     /gene="srsR"
                     /locus_tag="ACUQR0_001364"
                     /gene_synonym="ygfI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator SrsR"
                     /protein_id="HHV9108477.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14093..15571)
                     /gene="scpC"
                     /locus_tag="ACUQR0_001365"
     CDS             complement(14093..15571)
                     /gene="scpC"
                     /locus_tag="ACUQR0_001365"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HHV9108478.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15595..16371)
                     /gene="scpB"
                     /locus_tag="ACUQR0_001366"
     CDS             complement(15595..16371)
                     /gene="scpB"
                     /locus_tag="ACUQR0_001366"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HHV9108479.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16382..17377)
                     /gene="meaB"
                     /locus_tag="ACUQR0_001367"
     CDS             complement(16382..17377)
                     /gene="meaB"
                     /locus_tag="ACUQR0_001367"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HHV9108480.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17370..19514)
                     /gene="scpA"
                     /locus_tag="ACUQR0_001368"
     CDS             complement(17370..19514)
                     /gene="scpA"
                     /locus_tag="ACUQR0_001368"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HHV9108481.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19718..20611)
                     /gene="argP"
                     /locus_tag="ACUQR0_001369"
     CDS             complement(19718..20611)
                     /gene="argP"
                     /locus_tag="ACUQR0_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HHV9108482.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20994..21653
                     /gene="rpiA"
                     /locus_tag="ACUQR0_001370"
     CDS             20994..21653
                     /gene="rpiA"
                     /locus_tag="ACUQR0_001370"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HHV9108483.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21909..23141
                     /gene="serA"
                     /locus_tag="ACUQR0_001371"
     CDS             21909..23141
                     /gene="serA"
                     /locus_tag="ACUQR0_001371"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HHV9108484.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23370..23429
                     /gene="ibsC"
                     /locus_tag="ACUQR0_001372"
     CDS             23370..23429
                     /gene="ibsC"
                     /locus_tag="ACUQR0_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HHV9108485.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23762..23821
                     /locus_tag="ACUQR0_001373"
     CDS             23762..23821
                     /locus_tag="ACUQR0_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HHV9108486.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23922..24524)
                     /locus_tag="ACUQR0_001374"
     CDS             complement(23922..24524)
                     /locus_tag="ACUQR0_001374"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HHV9108487.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24545..24728)
                     /gene="ssrS"
                     /locus_tag="ACUQR0_001375"
     ncRNA           complement(24545..24728)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACUQR0_001375"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24770..25099)
                     /gene="zapA"
                     /locus_tag="ACUQR0_001376"
     CDS             complement(24770..25099)
                     /gene="zapA"
                     /locus_tag="ACUQR0_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HHV9108488.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25267..25845
                     /gene="ygfB"
                     /locus_tag="ACUQR0_001377"
     CDS             25267..25845
                     /gene="ygfB"
                     /locus_tag="ACUQR0_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HHV9108489.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25871..27196
                     /gene="pepP"
                     /locus_tag="ACUQR0_001378"
     CDS             25871..27196
                     /gene="pepP"
                     /locus_tag="ACUQR0_001378"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HHV9108490.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27193..28371
                     /gene="ubiH"
                     /locus_tag="ACUQR0_001379"
                     /gene_synonym="visB"
     CDS             27193..28371
                     /gene="ubiH"
                     /locus_tag="ACUQR0_001379"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HHV9108491.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28394..29596
                     /gene="ubiI"
                     /locus_tag="ACUQR0_001380"
                     /gene_synonym="visC"
     CDS             28394..29596
                     /gene="ubiI"
                     /locus_tag="ACUQR0_001380"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HHV9108492.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29670..29753)
                     /locus_tag="ACUQR0_001381"
     CDS             complement(29670..29753)
                     /locus_tag="ACUQR0_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108493.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30044..31138
                     /gene="gcvT"
                     /locus_tag="ACUQR0_001382"
     CDS             30044..31138
                     /gene="gcvT"
                     /locus_tag="ACUQR0_001382"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HHV9108494.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31162..31551
                     /gene="gcvH"
                     /locus_tag="ACUQR0_001383"
     CDS             31162..31551
                     /gene="gcvH"
                     /locus_tag="ACUQR0_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HHV9108495.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31670..34543
                     /gene="gcvP"
                     /locus_tag="ACUQR0_001384"
     CDS             31670..34543
                     /gene="gcvP"
                     /locus_tag="ACUQR0_001384"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HHV9108496.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34810..35552
                     /locus_tag="ACUQR0_001385"
                     /pseudo
     CDS             34810..35552
                     /locus_tag="ACUQR0_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35609..37042)
                     /gene="bglA"
                     /locus_tag="ACUQR0_001386"
     CDS             complement(35609..37042)
                     /gene="bglA"
                     /locus_tag="ACUQR0_001386"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HHV9108497.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37087..37397
                     /gene="yqfB"
                     /locus_tag="ACUQR0_001387"
                     /pseudo
     CDS             37087..37397
                     /gene="yqfB"
                     /locus_tag="ACUQR0_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37561..38220
                     /gene="trhA"
                     /locus_tag="ACUQR0_001388"
     CDS             37561..38220
                     /gene="trhA"
                     /locus_tag="ACUQR0_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HHV9108498.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
BASE COUNT         9107 a         9585 c        10332 g         9229 t
ORIGIN      
        1 tgaactgcca gaaattaccg ataatgaact gttagtgagt gtaatttctg acagcgtctg
       61 tttatcgacc tggaaagcgg cgttactcgg tagtgaacat aaacgcgtac ccgacgattt
      121 agaaaatcat ccggtcatta ccgggcatga atgtgccggg gttattgtcg aagtgggtaa
      181 aaatctcact ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt
      241 accaagcgga tattcagcgg gctacagcta cgaatatttt ggcggcaatg ccacttatat
      301 gattattccc gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg gctcttattt
      361 tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta
      421 tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggcg gcaatattgc
      481 actgctggcg tgtgcgggtc cgatgggcat tggcgctatc gattacgcca ttaacggtgg
      541 catacaaccg tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca
      601 gaagctgctg ccggtggatc tggcggccag taaaggcatt gagctagtgt atgtgaatac
      661 caaagggatg agcgatccta tccagatgct gcgggcgctg acaggagatg tcgggttcga
      721 tgacattttt gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg aattactggc
      781 ggaagatggc tgtctgaact tctttgccgg gctgacggat aaaaacttca aagtgccgtt
      841 taatttctac aacgtccatt acaacagcac gcacgtagtc ggtacatccg gcggttcaac
      901 ggacgacatg aaagaggcga ttgcccttag cgccacagga cagttacagc cgtcgtttat
      961 ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc tgccggatat
     1021 ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctcactg ccattgccga
     1081 ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc tggttgagga
     1141 aacgcacggc atctggaatg aacaggccga gaaatatctg ctggcacaat ttggcgttga
     1201 tatcggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt tacggaacag
     1261 gcagcgttgg ctgcctggcc gcacaccgga tgtggcgaca aaaataaaat tgatggcctg
     1321 gcggtcaccg cgatgcgcca ggcattaaac gacgtcgctt ttcgtggacg agtggttatc
     1381 ggcgaaggag aaattgacca tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc
     1441 gatgggccag aagtcgatat cgcggttgat cccattgaag gcacgcggat ggtggcgatg
     1501 gggcagagca atgcgctggc ggtcatggct ttcgctcctc gagatagcct gttacatgcg
     1561 cccgatatgt atatgaaaaa gctggttgtt aatcgactgg ctgccggggc gattgatctg
     1621 tctttgcctc tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa accgctggac
     1681 aagctgcgca tggttacgct ggataaaccg cgtctgagtg ccgcgattga agaagcaaca
     1741 cagctgggcg tgaaggtttt tgccctgccg gatggcgatg tcgccgccag cgtgctgacc
     1801 tgctggcagg ataatcctta cgatgtgatg tacaccatcg gcggtgcgcc agaaggcgtg
     1861 atttctgcct gtgccgttaa agcgttaggc ggggatatgc aggccgagct gattgacttt
     1921 tgtcaggcta aaggggatta cacggaaaat cggcagattg cagagcagga gcgcaagcgt
     1981 tgtaaggcaa tgggcgttga cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg
     2041 aacgatatcc tctttagcgc tacgggcgtg acgggcggcg agttggtgaa cggtatccaa
     2101 cagacggcga atggggtgcg gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt
     2161 aatataatag actccctgca ttgatggtga tctatcggaa gaccaatggc gacgctgaca
     2221 gaagatgatg tgcttgagca actggatgca caggacaatt tattttcatt tatgaaaact
     2281 gcgcattcta ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac
     2341 gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac
     2401 gatattgatg ttgcgctgcg tttgatttat gcgctgggga aaatggataa atggctgtat
     2461 gccgatatca cgcacttttc ccagtactgg cattacctga acgaacagga tgaaacgccc
     2521 ggatttgccg atgatataac ctgggatttt atcagcaatg tcaacagtat aactcgcaat
     2581 gcgacgctct acgatgcgtt aaaagcgatg aagttcgccg atttcgccgt ctggtcagag
     2641 gcgcgtttta gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag
     2701 gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc
     2761 atgatgaaga gatagaacgc gtgcataaac tgttgctgcg tatgctggcg gccttacaga
     2821 cggtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat
     2881 ctaccctaac caccttctgg gaatatctcg cgcaacaaga cccggagctg cccgctattc
     2941 aggcgctccc gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc
     3001 gccccttcaa aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc
     3061 aggtggtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg
     3121 aagatgcgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg
     3181 acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc
     3241 cagctgctgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg
     3301 cagatgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag
     3361 agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg
     3421 attaacgttc ctgattacac catcttccag ccgcaccaca cgagagaaat gacgccgggt
     3481 aaatgcggcg tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag
     3541 ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag
     3601 taatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcact
     3661 atgcagatcc aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc
     3721 atgggtacgt tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat
     3781 ctcgtcagcg acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gaacagtacg
     3841 ccaacctttg ccgcgcgctg gcggtttttc aggtttttca tcgcctgttg ttgcagcata
     3901 accgtaccgg ctgtgggcgt aagaagacct gccattactc gtaacagcgt cgatttccct
     3961 gcgccattgt caccggtcag cgccaaccat tcaccttgtt tgagttgcag agaaatgtcg
     4021 caaagacaat ccgttgccgc gccaggccag cgataagata tcttatttaa cgttaacata
     4081 gtggctggag cgctccgttt tgcaattgcc acgcttgctg gcagtagttc aggaaaggtg
     4141 tgcgatgacg ttcaaaaaga ataatcagcg aaccacgttc aaacgcccag tgttgtaagc
     4201 gctgcaacag catttcgctg gcctgcggcg tcaggcggct aaaggcttca tcaagaatta
     4261 agagctttgg ctgcatcgcg atggcgcagg cgatgatcac gcgctgtgtc tctccaccgg
     4321 agagtgttgc cgggtgacga tggcgcaacg gctggcactc ggtcagagtc agggcggcat
     4381 caatccgcgc cataatctct gcttcagcaa gacacagatt ttctggccca aaggcaactt
     4441 cttcttccac gctaaaggta cagccggaga gctgcaaata gggcgattgc tggacgagct
     4501 ggatggtggc ggattgttca ttaagcggga gatgcccaat gggcgtaccg aggagcgtcc
     4561 ccgtgcctgt tatctctctt ggcagaaagt ccggatacca gcccgccatc agctgtgcca
     4621 gcgtactttt gccactgccg ttgtcaccaa agatggcgac catccccggt ttgacatagt
     4681 gaaagtcata acagaacgga ggatgcgttg agtgtgtcgg gcaataacgg aactgttcta
     4741 acgtaaccat atccacgctc cgaattctgt cagcatcagc aagatcatgg tgtaacgcgc
     4801 tacgcgttgt agggtgctat cggctggtgc ccacaacgtt gttcgtcggt tgtggatccg
     4861 aaatgcccgc atatccagcg ccgcaccacg gacagccaga tcgttaagcg cattgtgtgt
     4921 tagcggaata attaacgcgg gcatagcacg taaacgctga taccagccct catccagcgg
     4981 cacgccgcgg gcacgctgcg cttcatgaat aatcgctaat tgccgtttta actgctcaac
     5041 gaccaataac ggcccggcaa acagataagc gacgcctggc ggcaggcgag aagcaaacag
     5101 agcgcgaata aaacattgca cggggacgta ctgcattcac aactgtgagg ttgaaacaat
     5161 cgccagaatc agcaaccaga gcgtaatagc atgcgaccac cgttctggcg agcgtggcgt
     5221 gccgcttagc cattctggta gccagccacc gtgtaccagc cacagcccgg ctcccagcga
     5281 gaacatcagc cagacgacat attttgcccg ccgccgcgtg gcctttagcg caataaggca
     5341 gaaaaaggtc gccgcgctat agatcggcaa aatggtctgc gcgggcagga taagcgtggt
     5401 gcaggccgcc aatgcccaca gtgttaatga ggtaaatgga tgcatttagc gcacggcagc
     5461 cattgccgga aaatggcgcg tggtgcgaat cggcagctgg cgcagtaaaa gccagacaat
     5521 caccgctgta agaattttat cgaccaggtt cgcgcctatc accgtaatcg ccaccgactc
     5581 caccaggttt tgccccatcg agtgcatcca ggcaacgaac aaatcggctc cgctaccggt
     5641 gacaccgccg aacagcgcag tacgcagcgg tacagcgacg accgttaccg caagcgtaat
     5701 aatcacgccg ctgacaacga cttttggcag ggtgcgaaac caacccgctc gcgccagcca
     5761 accggagacc aggccgatga ccatcgcgac gggtgcaaat gcggcggcga tcgggtcggt
     5821 cagcaatccc cagagtaaat tggtcagtaa gccggtcagc attccgatca ccggaccaag
     5881 cagtaccgcg ctaataagtg tgccgatgga gtcgagaaat ataggtaatt tgaccatgct
     5941 ggccagttgc ccgccgatca tattgatggc gatagatata acgatcaaca ccagtgcctg
     6001 actggaaaag tggctacgcg ccataagaaa acctctttga aagtagttat ttaacggggg
     6061 aaatatgcaa ctcgtcgtaa ccagaaagcg cgcaaggttg gcggctaaac gccagttcct
     6121 caaggctacc gatcaccagt tcaagcgtgg tccgcacggt acagccgggc atcatatgac
     6181 cgccaagcat cgtgccgtgt ggatcggaaa cgcagagatg aagatgctca ccactctgtt
     6241 ccagcgtgcc gtttagtgag atcacttcga atgtaccgtt caggagcgtg gtgccttctt
     6301 gcccggcata gcgcaaggcg acgtcagtca ggctaccggt acagcctgca atctaggcgg
     6361 catgaagttg atgttgctgt gcaaaagcgt gtagctggga aaaaacttcc tgcccgggga
     6421 ggaggcgcag tgcgtaaaag cgtgcggagg aagaatatga gtagggcaat gtcatcattg
     6481 gagtactccg aaactcggac gtattgcgga taatatcgac gctgcatcag tcatattctg
     6541 atataagtca gggtttgtga cggcacaacg aaaagatttt gctacgctta ttcgtgattc
     6601 tgcaaagtgg aataaattcc acaggggcgg ggctgaaaac gtgacgtaag tcagataatt
     6661 gctttctttg actggacaat cattcctttt attccacgtt tcgcttatcc tagctgaagc
     6721 gtttcagtcg attaaatgtt cgacaattaa ccaatcagtc gcagtttgcg acaggtaagg
     6781 tttccccgga cgatttgctg gattactctg tctggcaaat ttgctcaggg aaaaccttgc
     6841 aggagatcta tgaccgtacg cgtagcgata aatggcttcg gtcgcatcgg gcgtaatgtg
     6901 gttcgtgctt tgtatgaatc cggacgccgg gcggaaatta ccgtggtggc aatcaacgaa
     6961 ctggcggatg ctgcgggcat ggcgcatttg ttgaaatatg acaccagcca tggccgtttt
     7021 gcatgggaag tacgacagga acgcgatcaa ctttttgttg gtgatgacgc catccgcgta
     7081 ttgcatgaac gttcactgca atcgctcccc tggcgtgaac ttggcgttga tgtagtcctc
     7141 gactgcaccg gcgtatatgg ctcccgcgag catggcgaag cgcatattgc cgccggggcc
     7201 aaaaaagtgc tcttttcaca tcctggcagt aacgatctcg acgcgaccgt tgtttacggc
     7261 gtcaatcagg atcaacttcg tgcggaacac cgcatcgttt ctaacgcttc ctgtaccacg
     7321 aattgcataa ttcccgtcat caaattgtta gatgatgcgt acggtattga gtccggcact
     7381 gtgaccacaa ttcactccgc catgcacgat caacaggtta ttgatgcata ccatcctgac
     7441 ctgcgtcgca cccgggcagc cagccagtcg atcattccgg tcgatactaa actggccgct
     7501 ggtatcacac gattttttcc gcaatttaac gatcgctttg aagcgattgc ggtacgtgtg
     7561 ccaaccataa atgtgacggc aatcgattta agcgtgacgg tgaagaaacc tgtaaaagcc
     7621 aatgaagtca acctgttgct gcaaaaagca gcacaaggtg catttcatgg tatagttgac
     7681 tatacggaat tgccgttggt ctctgtagat tttaaccacg atccgcacag tgccattgtc
     7741 gatggcaccc aaacccgggt cagtggcgca cacctgatca aaacgttggt ctggtgcgat
     7801 aacgaatggg gctttgctaa ccgaatgctc gacacgacgt tagctatggc tactgttgct
     7861 ttcaggtaag acgcaagcag cgtctgcaaa acttttagaa tcaacgagag gattcaccat
     7921 gtctgtaatt aagatgaccg atctggatct tgctgggaaa cgtgtattta tccgtgcgga
     7981 tctgaacgta ccagtaaaag acgggaaagt aaccagcgac gcgcgtatcc gtgcttctct
     8041 gccgaccatc gaactggccc tgaaacaagg cgcaaaagtg atggtaactt cccacctggg
     8101 tcgtcctacc gaaggcgagt acaacgaaga attctctctg ctgccggttg ttaactacct
     8161 gaaagacaaa ctgtctaacc cggttcgtct ggttaaagat tacctcgacg gcgttgacgt
     8221 tgctgaaggt gaactggttg ttctggaaaa cgttcgcttc aacaaaggcg agaagaaaga
     8281 cgacgaaacc ctgtccaaaa aatacgctgc actgtgtgac gtgttcgtaa tggacgcatt
     8341 cggtactgct caccgcgcgc aggcttctac tcacggtatc ggtaaattcg ctgacgttgc
     8401 gtgcgcaggc ccgctgctgg cagctgaact ggacgcgctg ggtaaagcac tgaaagaacc
     8461 tgctcgcccg atggtggcta tcgttggtgg ttctaaagta tctaccaaac tgaccgttct
     8521 ggactccctg tctaaaatcg ctgaccagct gattgttggt ggtggtatcg ctaacacctt
     8581 tatcgcggca caaggccacg atgtgggtaa atccctgtac gaagctgacc tggttgacga
     8641 agctaaacgt ctgctgacca cctgcaacat cccggttccg tctgatgttc gcgtagcaac
     8701 cgagttctct gaaactgcac cggctaccct gaaatctgtt aacgatgtga aagctgacga
     8761 gcagatcctg gatatcggtg atgcttccgc tcaggaactg gctgaaatcc tgaagaatgc
     8821 gaaaaccatt ctgtggaacg gtccggttgg cgtgttcgaa ttcccgaact tccgcaaagg
     8881 tactgaaatc gtggctaacg ctatcgcaga cagcgaagcg ttctccatcg ctggcggcgg
     8941 cgacactctg gcagcaatcg acctgttcgg cattgctgac aaaatctcct acatctccac
     9001 tggcggcggc gcattcctcg aattcgtgga aggtaaagta ctgcctgcag tagcgatgct
     9061 cgaagagcgc gctaagaagt aaaaaatcac agggcaggga aacctgcact tgtttcagcg
     9121 cgcttttaga gcacgcattt tttcatactc taaataattc gagttgcagg aaggcgacaa
     9181 gagagtgaat ccccaggagc ttacataagt aagtgactgg ggggagcgaa cgcagacgca
     9241 gcacatgcaa cttgaagtat tatgagtata aggcccgacg atacaggaca agagacatgt
     9301 ctaagatttt tgatttcgta aaacctggcg taatcactgg tgatgacgta cagaaagttt
     9361 tccaggtagc aaaagaaaac aacttcgcac tgccagcagt aaactgcgtc ggtactgact
     9421 ccatcaacgc cgtactggaa actgctgcta aagttaaagc gccggttatc gttcagttct
     9481 ccaacggtgg tgcttccttt atcgctggta aaggcgtgaa atctgacgtt ccgcagggtg
     9541 ctgctatcct gggcgcgatc tctggtgcgc atcacgttca ccagatggct gaacattatg
     9601 gtgttccggt tatcctgcac actgaccact gcgcgaagaa actgctgccg tggatcgacg
     9661 gtctgctgga cgcgggtgaa aaacacttcg cagctaccgg taagccgctg ttctcttctc
     9721 acatgatcga cctgtctgaa gaatctctgc aagagaacat cgaaatctgc tctaaatacc
     9781 tggagcgtat gtccaaaatc ggcatgactc tggaaatcga actgggttgc accggtggtg
     9841 aagaagacgg cgtggacaac agccacatgg acgcttctgc actgtacacc cagccggaag
     9901 acgttgatta cgcatacacc gagctgagca aaatcagccc gcgtttcacc atcgctgcgt
     9961 ccttcggtaa cgtacacggt gtttacaagc cgggtaacgt ggttctgact ccgaccatcc
    10021 tgcgtgattc tcaggaatat gtttctaaga aacacaacct gccgcacaac agcctgaact
    10081 tcgtattcca cggtggttcc ggttctactg ctcaggaaat caaagactcc gtaagctacg
    10141 gcgtagtgaa aatgaacatc gataccgata cccaatgggc aacctgggaa ggcgttctga
    10201 actactacaa agcgaacgaa gcttatctgc agggtcagct gggtaacccg aaaggcgaag
    10261 atcagccgaa caagaaatac tacgatccgc gcgtatggct gcgtgccggt cagacttcga
    10321 tgatcgctcg tctggaaaaa gcattccagg aactgaacgc gatcgacgtt ctgtaagtca
    10381 ttcctttctg cttatctcaa ggcccgctct gcgggtcttt ttttcgccaa aagcaaggtg
    10441 attcagatga gaaatctgtg atctatttgg caaaattatg ctttattgtt tacccttgtc
    10501 agactgcccg tcataaggcg gcggagtgta tttctccatt ttgagtcagt tgaaaaggaa
    10561 tattgaatgg aagatttgaa tgttgtcgat agcataaacg gcgcgggaag ctggctggta
    10621 gctaaccagg cgctgctgct aagttatgca gtaaacatcg tggcggcact cgcgatcatc
    10681 atcgttggtt tgattatcgc gcggatgatt tccaacgcgg tgaatcgcct gatgatctcc
    10741 cgtaaaatcg atgccactgt tgctgatttt ctttctgcat tagtccgtta cggtattatc
    10801 gcctttacgc taatcgctgc cctgggacgc gtgggtgtac aaacagcgtc agtcattgct
    10861 gtactcggtg ccgcaggctt agctgttggt ctggctttgc aggggtcact ttctaacctg
    10921 gccgctggcg tgttacttgt catgttccgc ccgttccgtg ccggagaata tgttgacctc
    10981 ggcggcgtag ccggtactgt gctgagtgtg cagattttct ccaccaccat gcgtactgca
    11041 gacggtaaaa ttatcgttat tccgaacggt aaaattattg ccggaaatat tattaacttc
    11101 tcccgcgagc cagttcgccg taacgaattt attattggcg tggcgtatga ttccgatatc
    11161 gatcaggtta agcagatcct gaccaatatt atccagtctg aagatcgcat tttgaaagat
    11221 cgcgaaatga ctgtgcgcct gaacgaactt ggtgcatcgt cgattaattt cgtggtccgc
    11281 gtctggagca acagcggcga tctgcaaaac gtgtactggg atgtgctgga gcgtattaaa
    11341 cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc aaatggatgt gaactttaag
    11401 cgggtgaaag aagacaaagc tgcgtaatca acgctgaggc cagataatac tctctggcct
    11461 ctctcttatt agttattctg attgccaatt aatattatca atttccgcta ataacaatcc
    11521 cgcgatatag tctctgcatc agatacttaa ttcggaatat ccaacgtgtt ttcttattac
    11581 tttcaaggtc ttgctcttgg ggcagctatg attctgccgc tcggcccaca aaatgctttt
    11641 gtgatgaatc agggcattcg tcgtcagtac cacattatga ttgccttact ttgcgctatc
    11701 agcgatttgg tcctgatttg cgcggggatt tttggtggta gcgcgttatt gatgcagtcg
    11761 ccgtggttgc tagcgctggt cacctggggc ggcgtagtct tcttgctctg gtatggtttt
    11821 ggcgctttta aaacagcgat gagtagtaat attgagttag ctagtgccga agtcctgaag
    11881 caaggcagat ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt
    11941 tacctggata cttttgttgt actgggcagc cttggcgggc agcttgacgt cgaaccaaaa
    12001 cgttggtttg cgctcgggac aattagcgcc tctttcctgt ggttctttgg tctggctatt
    12061 ctcgcagcct ggctggcacc gcgcctgcgc acggcaaaat cacagcgcat tatcaatctg
    12121 gtagtgggat gtgtcatgtg gtttatagcc ttgcagctgg cgagagacgg tattgctcat
    12181 gcacaagcct tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct
    12241 tgcctccaga ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt
    12301 tcaaagttat cgccctggcg gcattaatgg gtattagcgg gatggcagcg caggctaacg
    12361 aattgccgga tggaccgcat attgtcacct ccggtacggc aagtgtggat gcggtgccag
    12421 acattgccac tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct acagccaaga
    12481 aacaggcaga tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga
    12541 aaaaagatat cagctcagcg aatttacgca cccagccaga ttatgactat caggatggta
    12601 aaagtatcct gaaaggctac cgagctgtga gaacggtgga agtcacgctc cgtcagttag
    12661 acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttctg
    12721 tgtcgctggg cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg
    12781 ataacgcgat tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg
    12841 tatatagcgt gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga
    12901 aagccgatgc cgcgccggtg tctgcccagg aaacttacga gcaggccgct attcagtttg
    12961 atgatcaggt cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac
    13021 ctgcagcaca ataatacttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata
    13081 ttgcctcctt tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc
    13141 ggtttcatca aggataggct gactgaaacg gttaagcgga caaggatgcg gggagggggg
    13201 atggacattt ttatttcgaa aaagatgcgc aatttcattt tattagcgca aaccaataat
    13261 attgcccggg cggcagaaaa aatccatatg acggcttcgc catttggtaa aagcattgcc
    13321 gcgctggaag aacaaattgg ctatacgcta tttacccgca aagataataa tatcagcctc
    13381 aataaagccg gtcaggaact gtatcaaaaa ctgttcccgg tttatcaacg actttctgcg
    13441 attgataatg aaatccataa ctcagggcgt cgttcacgag aaattgtgat tggcatagat
    13501 aatacttatc cgacaattat tttcgatcag ttgattagtc tcggagataa gtatgaaggc
    13561 gtgactgccc agccagttga gttcagcgaa aacggcgtta tcgataatct ttttgaccgt
    13621 cagttagatt ttattatctc cccacagcat gtatctgcac gagttcagga actggaaaac
    13681 ctgactatca gtgaattacc accgctgcga ttgggctttc tcgtttctcg ccgttatgaa
    13741 gagcgtcaag agcaagagct gcttcaggag ctgccctggc tacaaatgcg ctttcaaaac
    13801 cgggctaact tcgaagcaat gattgatgcg aatatgcgac cttgtgggat caatcccacc
    13861 attatttatc gtccctacag ttttatggcc aaaatcagcg ccgtggagcg cggacatttt
    13921 ctgacggtga tcccgcattt tgcctggcga ctggtgaatc cggcaacgct gaaatatttt
    13981 gatgcgccgc acaggccgat gtatatgcag gaatatcttt attcaatcag aaatcatcgt
    14041 tataccgcca cgatcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
    14101 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
    14161 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
    14221 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
    14281 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
    14341 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
    14401 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
    14461 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
    14521 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
    14581 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
    14641 ttgtcaggct ggaggcgctg gccccgctga ttttgccggt ttccagtaaa tgcaccaccg
    14701 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
    14761 gtgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
    14821 gaatacgccc atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct
    14881 ggcacatggg attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga
    14941 cggcgacaat ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat
    15001 ggaagatcga cacgctattg cgccgcggtg gcgcgccagg aatcacaata tctgccagtt
    15061 ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat cactttcttc gctcgcagca
    15121 gccaggtcgg cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg
    15181 ccgatgcttc aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg
    15241 ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta
    15301 aactggacga tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat
    15361 cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg aatttgatac ggctttttgg
    15421 cctcatgctg ttcgttagct ctgcgggcaa tcgcggcggg tagggctttc ggcgaacccg
    15481 ctggggtaaa gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat
    15541 cggcggtcat ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg
    15601 accaacgaaa ttaggtttac gtttttcgag gaaagcgttc atcccttcct ggtaatcttc
    15661 gctgtcataa accgcgcggc gcatcccctg aatacgttca aattcatcgg agttcatggt
    15721 gtgtgcttcg cccagtacac gcagctcttc tttgataacg gcaatggcta acggcgcttt
    15781 ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc acttccacaa catggttgag
    15841 gatgccgaca gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt
    15901 gacaatgtgg aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac
    15961 gccgaggttt acaggcgtca ttgagaaggt tgaggtactg gcggcgatga tcagatcgga
    16021 actcatgatc atttcaaatg cgccacccca aacactacct tccaccatcg aaatgatcgg
    16081 tttcgggaat ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg
    16141 atcgcgaccg ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc
    16201 acttggcgcc cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg
    16261 cataagatca tcaataaaga ctttacttaa ggcattaagt tttcgtccat agttaaactc
    16321 aatgaccgcc actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc
    16381 tttaatcaaa atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg
    16441 agagcgtatt gtttttgacc gctaaaagcg tctggtggta atagcgatcg aaatcttcat
    16501 tcgcgaacag gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt
    16561 gttgttgccg cacttgttgt aaacgaccac tggcagttag cgcggttttg aagtcgatga
    16621 tggcgtgcca gatctcatcg attccacgtt tttccagtgc gctacaagtc agaccccgtg
    16681 gctgccattc gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg
    16741 caatggcgac attggtatgg ttatcgccat cgtctttgtt gataacgagc agatcagcca
    16801 cttccatcag ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg
    16861 agataaaaca gtccaccatg cgggcgactt ccgtttccga ctgcgcgacg ccaaccgttt
    16921 cgacaatcac tacgtcataa cccgccgctt cgcataacag cattaattcc cgtgctcgct
    16981 gactggcacc gcccagatga ccggaggagg gtaccgggcg aataaacgcc gcctcggcac
    17041 gcgccaggtc attcatgcgg gttttatccc cgagaatgct accgccagtg accgggctgc
    17101 tgggatcgac cgcaataaca gcaaccttta atccctcacg aatcaacaac atgccaaagg
    17161 cttcaagaaa ggtacttttc cccgcgccgg gtgtgccggt aacgcccagt cgcagggcgt
    17221 taccacagaa cggcataata gcgtcaagca gctgtgtact tagcgcctga tgacgtgggt
    17281 gacggctttc caccagcgtc atggcttggg cgagcgtggc acgctcaccc tgacgtaagc
    17341 ggcgaatact ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt
    17401 acgtcgcgca cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc
    17461 tcttgcagga aggcgtaatc ctgcggcgga atgacgccac ccgcgaccac gcagatatct
    17521 tcgcgtcccc attttttcag cgcttcgacc agttccggga tcagcgtttt atgaccggca
    17581 gccagtgagg atgcgcccac tacgtgaacg tcgttttcta cggccaggcg ggcgatctct
    17641 tcaggtgtag agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg
    17701 gcgatcactt tcgcgccgcg atcgtgtcca tcctggccca tcttagcgat cagaatgcgc
    17761 gggcgacgac cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg
    17821 gaggccgatt tctcagactg atgatagctt tgcgcaatca cgccggtaac acactggctt
    17881 ggcaccagat aacggtcgaa agcgacttcc agcgcatcgg aaatttcacc cagggtggcg
    17941 cgaacgcgag cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga
    18001 gtcagggcgt tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc
    18061 agcgaagcaa tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg
    18121 tcttcgtgat ccagtttgta cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc
    18181 agcgactgtt cgcgcgctga ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc
    18241 gctttcgcca tgccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg
    18301 atttgatcgg tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgtggcag
    18361 agttctgatt cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg
    18421 gtaggcaaac caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc
    18481 gtcgcagcca gtgcttcaat ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc
    18541 aggctccagc ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg atcctgtgcg
    18601 ccaaatccac tgaccgcttc gctccataaa taacgtgccg cacgcaacat ggcgacgttc
    18661 ataaacagat ccatgccgat accgaagaat aacgacaggc gaggagcgaa gtcatcaatt
    18721 ttcagtccgg cagagattgc tgctttgatg tactcaatcc catcggcgat cgtaaatgct
    18781 acctgctgca cgcagttggc acccgcttcc cccatgtggt aaccgctgat actgatggta
    18841 ttaaatcgcg gcatgttgcc ggaacaccag gcgatgatgt cggcgataat gcgcattgac
    18901 ggttttggtg ggtaaatata ggtgttgcgg cagaggtact ctttgagaat atcgttttga
    18961 atggtgccgg tcagtttatc aggtgtaaca ccttgctctt ctgcggcgac gatataaaac
    19021 gccagtactg gtagcactgc gccattcatg gtcatcgaaa ccgacatttt atccagcggg
    19081 atctggtcga acaggacttt catatcttcc acggtgtcga tagcgacgcc cgctttgccg
    19141 acgtcacccg ccacgcgtgg gttatcggag tcgtagccac ggtgggtggc aaggtcaaac
    19201 gcaacggaaa gacctttttg cccggcggcc aggttacggc gataaaaagc gttggactct
    19261 tttgctgttg aaaaaccagc atactgacgg atggtccacg gttgggcggt atacatagtg
    19321 gcacgcgggc cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac ctccagatta
    19381 tcgagatcgg cttcggtata cagcggcttg atggcgatcc cttccgcggt ttgctgaacc
    19441 agcgagtcga cagttttctc ccgacggctc aattccttgt tggcaagctg ttgccactcc
    19501 tgctcgttag acattatttg ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcttac
    19561 ggtgagcttt ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt
    19621 gccgcctgag atttgtctat tttgtgatgt gattcaagga atacaaaaag aagtgaggac
    19681 aacgcctgat atgtgctatc aggcattatt tgatggatta atcctgacga aggactttgt
    19741 gaccataatc gagtaacgca tcagtgactt tacgcatcat gcggctttca ggagcaaagc
    19801 ggtgccagta gagcatccgt cgttgaaata gcccaggcgt taagtcaatc agttcaccgc
    19861 tggccagctc tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag
    19921 caagttgtac gaacgcttct gaagaattaa cgatatggca gggtacgctg ccaggaggaa
    19981 gatcgaagtt ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca aacgcgacca
    20041 ctggcgcttt caataatgcc gaacgcgtta cgccgttagg aaaatatttt tcggcaaacg
    20101 gttttgagct gacgaacaga tagtcgagcg caccgagttt atcgacaaga caactcggca
    20161 gcgcctgatg ttgaatactc accgcgccga ccacttcgcc gcggcgcaga cgttcctgag
    20221 tacgggtttc atcttctacc tgcaagttga ggcggatagg cgaatcagcc aacacaggag
    20281 ccagtgccgg aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca
    20341 gcggagtcga accggtttgt tcatcgccca gccactcttc ttccagcaac tccacctggc
    20401 gcagcagtgc cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca
    20461 acagcggctg cccgaacata ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg
    20521 taatgcacag cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg
    20581 cctgtaatgt tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt
    20641 ttcctgcact atgacacaat tttgtgtcag gttgcatata ccgaagccgt cggcgggtaa
    20701 catttgtatc aatggttaaa cgcgcagctt aaaagatcag ggatttgcgc tattgcattt
    20761 cgcttagcga cttcgcgccc tggttatgtt gaatggcgtg gcgttattgc ctctgttcgc
    20821 accgtggagc ggcataccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc
    20881 cgccattgcg cgatatttta tggatgagtt aaccacccag cttgccaacg gggtctgaat
    20941 cgcttttttt gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc
    21001 aggatgaatt gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggtacca
    21061 ttgttggtgt aggtacaggt tccaccgccg cacactttat tgacgcgctc ggcacgatga
    21121 aaggccagat tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc
    21181 tcggcattca cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg
    21241 cagatgaaat caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg
    21301 aaaaaatcat tgcttcggtt gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg
    21361 ttgatattct gggtaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag
    21421 tggcgcgcca gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg
    21481 ataatggcaa cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg
    21541 aaaacgccat aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg
    21601 cggacgttgc gctgattggc acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg
    21661 ggggaacctc ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgctt
    21721 ttaccaggca attgtcgatt gctctaaata aatcctctaa accagcatat tcatccaaga
    21781 attacctttg cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat
    21841 tattttttga tatgttgaaa ggcggatgca aatccgcaca caacatttca aaagacagga
    21901 ttgggtaaat ggcaaaggta tcgctggaga aagacaagat taagtttctg ctggtagaag
    21961 gcgtgcacca aaaggcgctg gaaagccttc gtgcagctgg ttacaccaac atcgaatttc
    22021 acaaaggcgc gctggatgat gaacaattaa aagaatccat ccgcgatgcc cacttcatcg
    22081 gcctgcgatc ccgtacccat ctgactgaag acgtgatcaa cgccgcagaa aaactggtcg
    22141 ctattggctg tttctgtatc ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg
    22201 ggatcccggt atttaacgca ccgttctcaa atacgcgctc tgttgcggag ctggtgattg
    22261 gcgaactgct gctgctattg cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg
    22321 tgtggaacaa actggcggcg ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg
    22381 gctacggtca tattggtacg caattgggca ttctggctga atcgctggga atgtatgttt
    22441 acttttatga tattgaaaac aaactgccgc tgggcaacgc cactcaggta cagcatcttt
    22501 ctgacctgct gaatatgagc gatgtggtga gtctgcatgt accagagaat ccgtccacca
    22561 aaaatatgat gggcgcgaaa gagatttcgc taatgaagcc cggctcgctg ctgattaatg
    22621 cctcgcgcgg tactgtggtg gatattccgg cgctgtgtga tgcgctggcg agcaaacatc
    22681 tggcgggggc ggcaatcgac gtattcccga cggaaccggc gaccaatagc gatccattta
    22741 cctctccact gtgtgaattc gacaacgtcc ttctgacgcc acacattggc ggttcgactc
    22801 aggaagcgca ggagaatatc ggcttggaag ttgcgggtaa attgatcaag tattctgaca
    22861 atggctcaac gctctctgcg gtgaacttcc cggaagtctc gctgccactg catggtggtc
    22921 gtcgtctgat gcacatccac gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct
    22981 tcgccgagca gggcgtcaac atcgccgcgc aatatctaca aacttccgcc cagatgggtt
    23041 atgtagttat tgatattgaa gctgacgaag acgttgccga aaaagcgctg caggcaatga
    23101 aagctattcc gggtaccatt cgcgcccgtc tgctgtacta attccccttc tctgaaaatt
    23161 aacgggcagg tcactgactt gcccgttttt ttatcccttc tccacaccgt tgccgcgttg
    23221 cgcattctac tttgcgagtc tgctccagaa aataaatcaa agtcagatca atgcgttgtg
    23281 tttatttaat ggggatttac gatggcggga cagcatgggg ctgtaacggt aaagccctca
    23341 ccgaagcgag ggcttaaagg agaagggtta tgatgcgact tgtcatcata ctgattgtac
    23401 tgttactgat aagtttcgga gcttattaac agtcagtctc aagggaggag tcatcctccc
    23461 ttacccttac tcactaaatt aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg
    23521 tgcatcgccc tgaaccgaac tttttgatag ccacttcatc gcccacgttt actgccgtaa
    23581 tagatccttt ttcccaaaaa ttatctctgt tttgcatggg gtttttcgag cacgctcgaa
    23641 gaaaagattt aaaggtagat caatgcgttg tggttattta aagagagctt acgatagctg
    23701 ggcagcatgg ggctgtaacg gtaaagccct caccgaagcg agggcttgaa ggagaagggt
    23761 tatgatgcga cttgccatca tactgattgt actgttactc ataagtttcc cagcttatta
    23821 acagtcagtc acaggggagg agcaatcctc ccttaccctt actcactaaa ttaggttaaa
    23881 gaatcaacga tgtcaatcag ggcgatgcag gtgtatcgcc cttaccactc ccagactttc
    23941 gacggtgtaa ccaccgcagg aagagggata tcccactctt caacggggag tttttccacc
    24001 aactgacaat catgcgcata acccaccggt tgcgttttat agtgctgcca gttttgtaag
    24061 gtccgatcat aaaaaccacc gcccattccc aggcgctgac cgtactcatc aaaggcgacc
    24121 agcggtgtga tcagcacgtc taatcgggaa aggggtagca cgtcacgcac atccagtttt
    24181 ggctcatgga tcttcaacct gttcatcacc agttcgcttt gcggatggta attcaggaac
    24241 agcaaattac cggcactaaa gggatgcaaa actggaagat atacgcgctt accggcgcgc
    24301 cagagttgtt ctattagcgg ctgggtgtcg agttcaccat caaaagagag gaatacagcg
    24361 accgtatgcg ccatcaccac cgggggataa gtcatcatcc gggtagcggc ttgttgaccc
    24421 atttccagct gttgttccgg cgttaacgca cgacgacgtt gccgaatcat tttgcggatt
    24481 tcttgtcggg ataatgtcag tgggagctct ggtagttgcg tcatggctgg tgccagataa
    24541 gaagggaatc tccgagatgc cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga
    24601 atgtgtcgtc gcagttttaa ggcttctcgg acgaaccgag catgctcacc aaccgcggag
    24661 cgccacattc ttgtggtatg aaatatcggc tcaggggact ggcccgcttg cgaacatctc
    24721 agagaaattt tgtcttcacg gttactctac cacagtaaac cgaaaagtgt cattcaaagt
    24781 tttggttagt tttttcggtg atgcgacctt gttcaagtaa cgcttgttct atggtctgct
    24841 gcagcatccg aatacgctgt tccatacttg ccgcgtagtc acgagtcttt gctttttctt
    24901 gcgctaactc atagctgata ttcaatgcgg caatgaagac caactgttca gtatttgtga
    24961 ctctagtgcg ttctttcaga tcttgcaacc gttggttcag atcgtccgct gcctgattca
    25021 acgcatccct ttggtcaggc gggcagttca cacgcagtga acggccaaaa atttggatat
    25081 cgacgggttg tgcagacatg ccaccttcct gctgattgac tgcgctgcct tcgtctttag
    25141 acccatggtc tgcgaagggg cgacactata gctaccctga tgagaagaga caagcccttt
    25201 tctggtccac cagggcccaa agtggtagca tatcatgaat attcctccct ttgacgacga
    25261 atgcttatgt ctatacagaa cgaaatgcct ggttacaacg aaatgaacca gtatctgaac
    25321 caacaaggga cgggtctgac ccctgccgag atgcatggtt taatcagtgg gatgatatgt
    25381 ggcggtaacg atgacagctc atggctgccg ctacttcacg acctgacgaa cgaaggcatg
    25441 gctttcggtc atgagctggc acaggcactg cgtaaaatgc actctgccac cagcgatgcc
    25501 ctgcaggatg acggcttcct ttttcagctt tatctgcctg atggcgatga tgtcagcgtt
    25561 ttcgatcggg ctgatgcact ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt
    25621 acgcaaccga agctggacaa agtgaccggc gaaaccggtg aagccatcga cgatctgcgt
    25681 aacatcgcgc agctgggcta cgacgaagac gaagatcagg aagagcttga aatgtcgctt
    25741 gaagagatca tcgaatacgt tcgtgttgcc gcgctgttat gccacgacac ctttactcat
    25801 ccgcaaccga ccgcgccaga agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa
    25861 ggagagtgtt atgagtgaga tatcccggca agagtttcag cgtcgccgtc aggccctggt
    25921 ggagcaaatg caacccggca gcgccgcgct gatttttgct gcaccagaag taacacgtag
    25981 cgccgacagc gaatacccct atcgtcagaa cagtgacttc tggtacttca ccggctttaa
    26041 cgaaccggaa gcggtgctgg tgctgattaa aagcgatgac actcataacc acagcgttct
    26101 gtttaaccgc gttcgcgacc tgacggcgga gatctggttt ggccgtcgct taggccagga
    26161 tgccgcgcca gagaaactgg gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca
    26221 actttatcaa ctacttaacg gcctggatgt ggtttaccat gcccagggcg aatatgcata
    26281 tgctgatgaa atcgtgaaca gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct
    26341 caccgcaccg gcaacgatga tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa
    26401 atcgccagaa gagattgccg tactccgccg cgcgggagaa atcaccgcca tggcacatac
    26461 acgggcgatg gaaaaatgcc gtccgggaat gttcgagtac catctggaag gcgaaattca
    26521 ccacgaattt aaccgccacg gtgcgcgcta tccttcctat aacaccattg taggcagcgg
    26581 tgaaaacggc tgcattctgc actacaccga aaacgagagt gaactgcgcg acggtgacct
    26641 ggtgttgatt gacgcgggtt gtgaatacaa aggttacgcg ggcgatatta cccgcacctt
    26701 cccggtcaac ggcaaattca cccaggccca gcgtgaaatc tacgacattg tgctggagtc
    26761 tctcgaaacc agcctgcgcc tgtatcgtcc gggaacttcc attctggaag tcactggtga
    26821 agtggtgcgc atcatggtta gcggcctggt aaaactcggc atcctgaaag gtaatgttga
    26881 tgaactgatc gctcagaacg cccatcgtcc tttctttatg catggcctta gccactggtt
    26941 aggactggat gtccatgacg ttggcgttta tggtcaggat cgctcgcgca ttctggaacc
    27001 ggggatggta ctgaccgtag agcctggtct gtatatcgcg ccggatgcgg atgtgccaga
    27061 acaatatcgc ggtatcggca ttcgcattga agacgacatt gtgattaccg aaaccggcaa
    27121 cgaaaacctc accgccagcg tggtgaaaaa gccggaagaa atcgaagcgt tgatggctgc
    27181 tgcgagaaag caatgagcgt aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg
    27241 ctggctattt cccggttaag tcacggggcg ctgccggtac atttgattga agcgactgcg
    27301 ccagagtcac atgctcatcc gggctttgat ggacgagcga tagcgctggc ggcgggtacc
    27361 tgtcagcaac tggcgcgcat cggcgtctgg caatctctgg ctgattgcgc aaccgccatc
    27421 actaccgtgc atgtcagcga tcgtggtcat gctggattcg tcaccctcgc cgcagaagat
    27481 taccaactgg cggcgctggg gcaggttgtc gaattgcata atgtcgggca acggctgttt
    27541 gcattgctgc gaaaagcacc tggcgtaatg ctgcattgcc ccgatcgcgt agctaacgtt
    27601 gcccgtactc agagtcatgt tgaagtgacg ctggagagcg gcgagatgct gacgggccgc
    27661 gtgctgatag ccgctgatgg cacccattca gcgttagcca ccgcgtgcgg cgttgattgg
    27721 cagcaggagc cttacgaaca actggcagtg attgccaacg ttgctacttc tgttgcgcac
    27781 gaaggccgcg cttttgaacg cttcacgcaa catggcccgc tggcgatgtt gccgatgtct
    27841 gacggacgct gttcgctggt ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg
    27901 tggagcgacg agaagttttg ccgtgaactc cagtctgcct ttggctggcg actggggcaa
    27961 attacccacg ccggtaaacg cagtgcttat ccgctggcat taacccgcgc cgccagaccg
    28021 attacccatc gcactgtgct ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg
    28081 caagggttta acctcggtat gcgagatgtc atgagccttg cggaaaccct gactcaggcg
    28141 catgagcgcg gagaagacat cggtgattac ggcatattgt gccgctatca gcagcgtcga
    28201 cagagcgatc gcgaagcaac catcggcgtc acggacagcc tggtacatct ttttgccaac
    28261 cgttgggcac cgctggttgt cgggcgcaac atcggactga tgacgatgga attattcacc
    28321 ccggcacgcg atgtgctggc gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt
    28381 taaggaataa acaatgcaaa gtgttgatgt agccattgtt ggcggcggca tggtggggct
    28441 ggcggttgcc tgcggcttac aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt
    28501 accggaacct ctggcggtag atgcactacc acaactgcgc gtttcggcta tcaatgccgc
    28561 cagcgaaaaa ttactcaccc gtcttggcgt ctggcaggac attctcgctc gtagggccag
    28621 ttgttatcac ggcatggaag tgtgggacaa agacagtttt ggtcacatct cgtttgacga
    28681 tcaaagcatg ggctatagcc atcttgggca catcgttgaa aactcagtga ttcactacgc
    28741 gctgtggaac aaagcgcagc agtcgtcaga tattactctg ttggccccca cagaattaca
    28801 gcaggtcgcc tggggagaaa atgaaacctt cctgacgctg aaagatggca gcatgttaac
    28861 ggcgcgtctg gtgattggcg cggacggtgc taattcctgg ttgcgcaaca aagcagatat
    28921 tccgctgact ttctgggatt atcagcatca cgcgctggta gcgaccattc gcacggaaga
    28981 accgcatgat gcggtggcgc ggcaggtttt ccatggcgaa ggcattctgg cctttttacc
    29041 gcttagcgat ccgcatcttt gctcgattgt ctggtcactg tcgccagagg aagcgcagcg
    29101 gatgcagcag gcaagtgaag acgaatttaa tcgcgcgtta aatatcgctt ttgataatcg
    29161 ccttggctta tgcaaggttg agagcgcgcg tcaggtgttc ccgctgacgg ggcgttatgc
    29221 gcgccagttt gccgcacacc gtctggcgct ggtgggcgac gccgcgcata ccattcaccc
    29281 actggcgggg cagggcgtta atctcggatt tatggacgcc gcagagctga ttgctgaact
    29341 gaaacggttg catcgtcagg gtaaagacat cgggcagtac atttacctgc gccgctatga
    29401 acgtagccgc aagcacagcg cggcgctgat gctggctggt atgcagggtt ttcgcgatct
    29461 cttttccggc gctaatccgg cgaaaaaact gctgcgtgat attggtttga aactggccga
    29521 cacgcttcct ggcgttaagc cgcaacttat ccgccaggca atgggattaa acgatttgcc
    29581 tgaatggctg cgttaaaaat ttctcctctg ttgtttattt gatacccatc acactttcat
    29641 ctcccggttt tttcgccggg agattttcct catttgaaat aaactaattt cacctccgtt
    29701 ttcgcattat attttctaat accatgattt tttgatttag tgttttttga cattttttta
    29761 gctcttaata ttgtcttatt caaattgact ttctcatcac atcatctttg tatagaaatt
    29821 ggtgtatttt ttggtttttt attctgtcgc gatttttgca ttttttaacc ataagctaat
    29881 gtgatgatca attttacctt atggttaaca gtctgtttcg gtggtaagtt caggcaaaag
    29941 agaacgattg cgttggggac cgggagtggc tccgatgctg ggttttgtgg tgataatttc
    30001 accatgaaaa agttgtcagc cccgcttatt caatgaggac aagatggcac aacagactcc
    30061 tttgtacgaa caacacacgc tttgcggcgc tcgcatggtg gatttccacg gctggatgat
    30121 gccgctgcat tacggttcgc aaatcgacga acatcatgcg gtacgtaccg atgccggaat
    30181 gtttgatgtg tcgcatatga ccatcgtcga tctccgcggc agccgcaccc gggagtttct
    30241 gcgttatctg ctggcgaacg atgtggcgaa gctcaccaaa agaggcaaag ccctttactc
    30301 ggggatgttg aatgcctctg gcggtgtgat agatgatctc atcgtctact actttactga
    30361 agatttcttc cgcctcgttg ttaactccgc cacccgcgaa aaagacctct cctggattac
    30421 ccaacacgct gaacctttcg gcatcgaaat taccgttcgt gatgaccttt ccatgattgc
    30481 cgtgcaaggg ccgaatgctc aggcaaaagc tgccacactg tttaatgatg ctcagcgtca
    30541 ggcggtggaa gggatgaaac cgttctttgg cgtgcaggtg ggcgatctgt ttattgccac
    30601 cactggttat accggtgaag cgggctatga aattgcgctg cccaatgaaa aagcggccga
    30661 tttctggcgt gcgctggtgg aagcgggcgt taagccctgc ggtctggggg cgcgtgacac
    30721 gctgcgtctg gaagcgggca tgaatcttta tggtcaggag atggacgaaa ctatttctcc
    30781 tttagccgcc aacatgggct ggactatcgc ctgggaaccg acagatcgtg actttatcgg
    30841 tcgtgaagcc ctggaagtgc agcgtgagca tggtacagaa aaactggttg gtctggtgat
    30901 gaccgaaaaa ggcgtgctgc gtaatgaact gccggtacgc tttaccgatg cgcagggcaa
    30961 ccagcatgaa ggcattatca ccagcggtac tttctccccg acgctgggtt acagcattgc
    31021 gctggcgcgc gtgccggaag gtattggcga aacggcgatt gtgcaaattc gcaaccgtga
    31081 aatgccggtt aaagtgacaa aacctgtttt tgtgcgtaac ggcaaagccg tcgcgtgatt
    31141 tacttttttg gagattgatt gatgagcaac gtaccagcag aactgaaata cagcaaagaa
    31201 cacgaatggc tgcgtaaaga agccgacggc acttacaccg ttggtattac cgaacatgct
    31261 caggagctgt taggcgatat ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc
    31321 gcgggcgatg actgcgcggt tgccgaatcg gtaaaagcgg cgtcagacat ttatgcgcca
    31381 gtaagcggtg aaatcgtggc ggtaaacgac gcactgagcg attccccgga actggtgaac
    31441 agcgaaccgt atgcaggcgg ctggatcttt aaaatcaaag ccagcgatga aagcgaactg
    31501 gaatcactgc tggatgcgac cgcatacgaa gcattgttag aagatgagta acggctttat
    31561 tcctcttctg cgggagagga tcagggtgag gaaaatttat gcctcaccct cactctcttc
    31621 gtaaggagag aggttcacaa ttcactgcac gtttcaggaa ccatcgctca tgacacagac
    31681 gttaagccag cttgaaaaca gcggcgcttt tattgaacgc catatcggac cggacgccgc
    31741 gcaacagcaa gaaatgctga atgccgttgg tgcacaatcg ttaaacgcgc tgaccggcca
    31801 gattgtgccg aaagatattc agcttgcgac accaccgcag gttggcgcac cggcgaccga
    31861 atacgccgca ctggcagaac tcaaggctat tgccagtcgc aataaacgct tcacgtctta
    31921 catcggcatg ggttacaccg ccgtgcagct accgccggtt atcctgcgta acatgctgga
    31981 aaatccgggc tggtataccg cgtacactcc gtatcagcct gaagtctccc agggccgcct
    32041 tgaagcactg ctcaacttcc agcaggtaac gctggatttg actggactgg atatggcctc
    32101 tgcttctctt ctggacgagg ccaccgctgc cgccgaagca atggcgatgg cgaaacgcgt
    32161 cagcaaactg aaaaatgcca accgcttctt cgtggcttcc aatgtgcatc cgcaaacgct
    32221 ggatgtggtc cgtactcgtg ctgaaacctt tggttttgaa gtgattgtcg atgacgcgca
    32281 aaaagtgctc gaccatcagg acgtcttcgg cgtgctgtta cagcaggtag gcactaccgg
    32341 tgaaattcac gactacactg cgcttattag cgaactgaaa tcacgcaaaa ttgtggtcag
    32401 cgttgccgcc gatattatgg cgctggtgct gttaactgcg ccgggtaaac agggcgcgga
    32461 tattgttttt ggttcggcgc aacgcttcgg cgtgccgatg ggctacggtg gcccacacgc
    32521 ggcattcttt gcggcgaaag atgaatacaa acgctcaatg ccgggccgta ttatcggtgt
    32581 atcgaaagat gcagctggca ataccgcgct gcgcatggcg atgcagactc gcgagcaaca
    32641 tattcgtcgt gagaaagcga actccaacat ttgtacttcc caggtactgc tggcaaacat
    32701 cgccagcctg tatgccgttt atcacggccc ggttggcctg aaacgtatcg ctaaccgcat
    32761 tcaccgtctg accgatattc tggcggcggg cctgcaacaa aaaggtctga aactgcgcca
    32821 tgcgcactat ttcgacacct tgtgtgtgga agtggccgac aaagcgggcg tactgacgcg
    32881 tgccgaagcg gctgaaatca acctgcgtag cgatatcctg aacgccgtgg ggatcaccct
    32941 tgatgaaacc accacgcgtg aaaacgtaat gcagcttttc agtgtgctgc tgggcgataa
    33001 ccacgggctg gagatcgaca cgctggacaa agacgtggct cacgacagcc gctctatcca
    33061 gcctgcgatg ctgcgcgacg acgaaatcct cacccatccg gtgtttaatc gctatcacag
    33121 cgaaaccgaa atgatgcgct atatgcactc gctggagcgt aaagatctgg cgctgaatca
    33181 ggcgatgatc ccgctgggtt cctgcaccat gaaactgaac gccgccgccg agatgatccc
    33241 aatcacttgg acggaatttg ccgaactgca cccgttttgc ccgccggaac aggctgaagg
    33301 ttatcagcag atgattgcgc agctggctga ctggctggtg aaactgaccg gttacgacgc
    33361 cgtttgtatg cagccgaact ccggcgcaca gggcgaatac gcgggcctgc tggcgattcg
    33421 tcattatcat gaaagccgca acgaagggca tcgcgatatc tgcctgatcc cggcttctgc
    33481 gcacggaact aaccccgctt ctgcacatat ggcaggaatg caggtggtgg ttgtggcgtg
    33541 tgataaaaac ggcaacatcg atctgactga tctgcgcgcg aaagcggaac aggcgggcga
    33601 taacctctcc tgtatcatgg tgacttatcc ttctacccac ggcgtgtatg aagaaacgat
    33661 ccgtgaagtg tgtgaagtcg tgcatcagtt cggcggtcag gtttaccttg atggcgcgaa
    33721 catgaacgcc caggttggca tcacctcgcc gggctttatt ggcgcggacg tttcgcacct
    33781 caacctgcat aaaactttct gcattccgca cggcggtggt ggtccgggta tgggaccgat
    33841 cggtgtgaaa gcgcatctgg caccgtttgt accgggtcat agcgtggtgc aaatcgaagg
    33901 catgttaacc cgtcagggcg cagtttctgc ggcaccgttc ggtagcgcct ctatcctgcc
    33961 aatcagctgg atgtacatcc gcatgatggg cgcagaaggg ctgaaaaaag caagccaggt
    34021 ggcaatcctc aacgccaact atattgccag ccgcctgcag gatgccttcc cggtgctgta
    34081 taccggtcgc gacggtcgcg tggcgcacga atgtattctc gatattcgcc cgctgaaaga
    34141 agaaaccggc atcagcgagc tggatattgc caagcgcctg atcgactacg gtttccacgc
    34201 gccgacgatg tcgttcccgg tggcgggtac gctgatggtt gaaccgactg aatctgaaag
    34261 caaagtggaa ctggatcgct ttatcgacgc gatgctggct atccgcgcag aaattgacca
    34321 ggtgaaagcc ggtgtctggc cgctggaaga taacccgctg gtgaacgcgc cgcacattca
    34381 gagcgaactg gtcgccgagt gggcgcatcc gtacagccgt gaagttgcgg tattcccggc
    34441 aggcgtggca gacaaatact ggccgacggt gaaacgtctg gatgatgttt acggcgaccg
    34501 taacctgttc tgctcctgcg taccgattag cgaataccag taattcactg attcgagtat
    34561 tttctaaagg cgcttcggcg cctttttagt cagatgacaa agtacaaaag tgctcagaca
    34621 gtcccctcgc ccctttgggg agagggttag ggtgagggga acaggccggc actggcgcga
    34681 atatttaccc tcaccccgac cctctccctg aaagggcgag ggggaaaagc gtgccaacat
    34741 tgaagattga gccagtttgt tagcaatctc aaagatacgt caacgaatta atttttctcg
    34801 gaaaaacaaa tggctatagc acttgtgacc ggtggcagtc gcggcatcgg gcgggcaact
    34861 gtattactgt tggcgcaaga agggtatacg gtggcggtta attatcagca aaacctccac
    34921 gcggcgcagg aagtgatgaa cttaataatg caagccggtg gcaaagcatt cgtgctccag
    34981 gcggatatca gcgacgaaaa ccaggtcgtt gcgatgttta cagcaatcga tcagcacgat
    35041 gaaccgctag cagcgctggt caataacgcc gggatcttgt ttacccagtg caccgttgaa
    35101 aaccttaccg cagagcgaat caaccgagta ctttccacca acgtgacggg atattttctc
    35161 tgctgccgcg aggcggtaaa acgcatggcg cttaaaaatg gtggcagtgg cggcgctatc
    35221 gtcaatgtct cttcggtggc ctcacggttg ggttcgccag gggaatatgt tgattacgcg
    35281 gcatcgaaag gggcgattga ttcgttaacc accggactat cgctggaagt cgccgcgcag
    35341 gggatccgcg ttaactgcgt gcggccaggg tttatttata ccgaaatgca cgccagcgcg
    35401 gcgagcctgg acgcgtcgat cgcgttaagt cgaacatccc catgcagcgt ggtggacagg
    35461 cagaagaggt cgcgcaggcc attgtctggc tactaagtga taaagcctct tacgtcacgg
    35521 gaagttttat cgatttggcg ggcgggaaat aaaacaggga agttgtctga ccggatgcaa
    35581 caagtattgc atccggtact tcatcgactt aaagcttctc gccgttgctg gcaatcacct
    35641 ctttgtacca gttaaagctc ttcttacgtg aacgcgacat atcgccagta ccgtcgtcat
    35701 gtttattcac atagataaag ccgtagcgtt tgctgtactg cccggtagtg aatgacacgc
    35761 agtcgatgca gccccacggt gtgtagccca tcagatccac accatcgtaa gtcactgctt
    35821 ttttcatctc ttcaatatgg gcgcgcaggt agtcaatgcg gtagtcgtcg ttgatgctgc
    35881 catcttcttc cactttgtcg taagcgccaa aaccgttttc gacaataaac agcggcctct
    35941 gataacgctc atacagttcg caaagggcat agcgcagacc tactggatca atctgccagc
    36001 cccagtcaga cgctttaaca tacgggttcg gtacgctgcc ttcaaaacca gagatcgcat
    36061 cgccggtgcc gccttcggcc ttcactgcgt tggtcatgta atagctgaaa ccaagataat
    36121 cgcaggtgcc ttcgcgcagt acatcgagat cgccgtcttc cattttgata ttaaatccgc
    36181 gacgttccca ctcgttcaat acataggacg ggtaatagcc gcatagctgc acatcggtaa
    36241 agacgtagcg ttcgcgcatc gactcctgag cgaacatcac atcgtccggg ttacatgagt
    36301 agggatagag cggcaccatc gccagcatac agccgacttt catctccggg ttaatgcgac
    36361 gcgcggcttt cactgccagg gcgctggcga caaactggtg atgcagcacc tggtacatcg
    36421 tctcttccgg gttgtcatgc tcggtataca ccacgccgga gcagcagtaa ccaaacagcg
    36481 gcgcacgcca gttacgctgg ttgtttattt cgttgaaggt catccagtat ttgactttat
    36541 gcttgtagcg ttcgaatacc acttccgcga aacggacaaa gaaatcaacg aatttacggt
    36601 tggtccagct accgtattgc tgcaccagat gcagcggcat ttcaaagtgg gagagggtta
    36661 tcaccggttc gatgttgtat ttcagcagtt catcgaacag agagtcgtaa aacttcagcc
    36721 cttcttcgtt tggctgagct tcatcacctt ttggaaaaat acgcgtccag gcgatggaag
    36781 tccgaaaaca tttgaagccc atttcggcaa atagcttgat gtcttccttg tagtgaccat
    36841 aaaaatcaac ggcttcatgg tttggatagt attttcctgg caagacttct ttggtgattt
    36901 cgcgcggcac gccgtgtgcg ccaccggtca gaacgtcaca aatgctcggt cctttgccgc
    36961 ctttgttcca gccgccttcg acctgatgag cggcaacggc gccaccccat aagaaatctt
    37021 tcggtaaggt gagttttttc actatcatag gctccagatc agtcaattct tacaggaaat
    37081 agtaccatgc agccaaacga catcactttt ttcaacgttt ccaggatgac attctggctg
    37141 ggcgtaaaac catcaccatc cgcgacgagt ctgaatcgca cttcaaaacg ggtgatgtgc
    37201 ttcgtgtcgg acgttttgaa gatgacggtt atttttgcac gattgaagtc accgcaacct
    37261 caaccgtaac gctggataca ctgacggaaa aacatgcaga gcaggaaaat atgaccctgc
    37321 ctgaactgaa aaaggtcatt gctgacatct atcccggtca gacacaattt tatatgattg
    37381 aatttaaatg tctttaaatt gaactcacgc ctgttagttg aaatttcgca tttaatttca
    37441 tgatttttag gagattagtc tcaaatcaga atttatttta gctaacaggt gttcactgga
    37501 actattctca gttacgctgg agaggtatac atgtgcgtgt acgtttctct ggagtaagtt
    37561 atggttcaga agcccctcat taagcaggga tattcgctgg cagaggaaat tgccaacagc
    37621 gtcagtcacg gcattgggct ggtgtttggt atcgttgggc tggtgttgct actggttcag
    37681 gcggtggatc tcaatgccag cgccacagcg ataaccagtt acagcctcta tggcggcagt
    37741 atgatcctgc tgttcctcgc ttcgacgctc tatcacgcca ttcctcatca acgggcaaaa
    37801 atgtggctga agaaatttga ccattgcgct atttacctgt tgattgccgg aacctacacg
    37861 ccgtttttgc tggtggggct ggattctccg ttagcgcgcg ggttgatgat tgttatctgg
    37921 agcctagcat tgctggggat tctgttcaaa ctgaccatcg cgcaccgatt caaaatttta
    37981 tctctggtga cttatctggc gatgggctgg ctgtcgctgg tggtaattta tgaaatggca
    38041 gttaagctcg cggcgggcag cgttacctta ctggcggtag gcggcgtggt ttattcgctc
    38101 ggggtgattt tctacgtctg caaacgcatt ccatacaacc atgccatctg gcacggcttc
    38161 gtgctcggcg gtagtgtgtg ccactttctg gcgatctatt tgtatattgg gcaggcgtaa
    38221 tgggtatgcc tttgtaggcc tgataagacg cgt
//
LOCUS       DBKELA010000027        38156 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.027, whole genome shotgun sequence.
ACCESSION   DBKELA010000027 DBKELA010000000
VERSION     DBKELA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.027"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            97..996
                     /gene="hisG"
                     /locus_tag="ACUQR0_001389"
     CDS             97..996
                     /gene="hisG"
                     /locus_tag="ACUQR0_001389"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HHV9108499.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1002..2306
                     /gene="hisD"
                     /locus_tag="ACUQR0_001390"
     CDS             1002..2306
                     /gene="hisD"
                     /locus_tag="ACUQR0_001390"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HHV9108500.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2303..3373
                     /gene="hisC"
                     /locus_tag="ACUQR0_001391"
     CDS             2303..3373
                     /gene="hisC"
                     /locus_tag="ACUQR0_001391"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HHV9108501.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3373..4440
                     /gene="hisB"
                     /locus_tag="ACUQR0_001392"
     CDS             3373..4440
                     /gene="hisB"
                     /locus_tag="ACUQR0_001392"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HHV9108502.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAESMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4440..5030
                     /gene="hisH"
                     /locus_tag="ACUQR0_001393"
     CDS             4440..5030
                     /gene="hisH"
                     /locus_tag="ACUQR0_001393"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HHV9108503.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5030..5767
                     /gene="hisA"
                     /locus_tag="ACUQR0_001394"
     CDS             5030..5767
                     /gene="hisA"
                     /locus_tag="ACUQR0_001394"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HHV9108504.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5749..6525
                     /gene="hisF"
                     /locus_tag="ACUQR0_001395"
     CDS             5749..6525
                     /gene="hisF"
                     /locus_tag="ACUQR0_001395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HHV9108505.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6519..7130
                     /gene="hisIE"
                     /locus_tag="ACUQR0_001396"
     CDS             6519..7130
                     /gene="hisIE"
                     /locus_tag="ACUQR0_001396"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HHV9108506.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7316..8293)
                     /gene="wzzB"
                     /locus_tag="ACUQR0_001397"
     CDS             complement(7316..8293)
                     /gene="wzzB"
                     /locus_tag="ACUQR0_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HHV9108507.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8439..9559)
                     /gene="ugd"
                     /locus_tag="ACUQR0_001398"
                     /pseudo
     CDS             complement(8439..9559)
                     /gene="ugd"
                     /locus_tag="ACUQR0_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(9809..11215)
                     /gene="gndA"
                     /locus_tag="ACUQR0_001399"
     CDS             complement(9809..11215)
                     /gene="gndA"
                     /locus_tag="ACUQR0_001399"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HHV9108508.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11324..11638)
                     /locus_tag="ACUQR0_001400"
     CDS             complement(11324..11638)
                     /locus_tag="ACUQR0_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108509.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
     gene            complement(11680..12186)
                     /locus_tag="ACUQR0_001401"
     CDS             complement(11680..12186)
                     /locus_tag="ACUQR0_001401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108510.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12332..12931)
                     /locus_tag="ACUQR0_001402"
     CDS             complement(12332..12931)
                     /locus_tag="ACUQR0_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108511.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13102..14040)
                     /locus_tag="ACUQR0_001403"
                     /pseudo
     CDS             complement(13102..14040)
                     /locus_tag="ACUQR0_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(14103..14477)
                     /locus_tag="ACUQR0_001404"
     CDS             complement(14103..14477)
                     /locus_tag="ACUQR0_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HHV9108512.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14481..15629)
                     /gene="wzy"
                     /locus_tag="ACUQR0_001405"
     CDS             complement(14481..15629)
                     /gene="wzy"
                     /locus_tag="ACUQR0_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HHV9108513.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(15649..16554)
                     /gene="rfbG"
                     /locus_tag="ACUQR0_001406"
     CDS             complement(15649..16554)
                     /gene="rfbG"
                     /locus_tag="ACUQR0_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HHV9108514.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16547..17437)
                     /locus_tag="ACUQR0_001407"
     CDS             complement(16547..17437)
                     /locus_tag="ACUQR0_001407"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HHV9108515.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17467..18723)
                     /gene="wzx"
                     /locus_tag="ACUQR0_001408"
     CDS             complement(17467..18723)
                     /gene="wzx"
                     /locus_tag="ACUQR0_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HHV9108516.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(18739..19284)
                     /gene="rfbC"
                     /locus_tag="ACUQR0_001409"
     CDS             complement(18739..19284)
                     /gene="rfbC"
                     /locus_tag="ACUQR0_001409"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HHV9108517.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19289..20167)
                     /gene="rfbA"
                     /locus_tag="ACUQR0_001410"
     CDS             complement(19289..20167)
                     /gene="rfbA"
                     /locus_tag="ACUQR0_001410"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HHV9108518.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20226..21125)
                     /gene="rfbD"
                     /locus_tag="ACUQR0_001411"
     CDS             complement(20226..21125)
                     /gene="rfbD"
                     /locus_tag="ACUQR0_001411"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HHV9108519.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21125..22210)
                     /gene="rfbB"
                     /locus_tag="ACUQR0_001412"
     CDS             complement(21125..22210)
                     /gene="rfbB"
                     /locus_tag="ACUQR0_001412"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HHV9108520.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(22583..23476)
                     /gene="galF"
                     /locus_tag="ACUQR0_001413"
     CDS             complement(22583..23476)
                     /gene="galF"
                     /locus_tag="ACUQR0_001413"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HHV9108521.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(23651..25045)
                     /gene="wcaM"
                     /locus_tag="ACUQR0_001414"
     CDS             complement(23651..25045)
                     /gene="wcaM"
                     /locus_tag="ACUQR0_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HHV9108522.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25056..26276)
                     /gene="wcaL"
                     /locus_tag="ACUQR0_001415"
     CDS             complement(25056..26276)
                     /gene="wcaL"
                     /locus_tag="ACUQR0_001415"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HHV9108523.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26273..27553)
                     /gene="wcaK"
                     /locus_tag="ACUQR0_001416"
     CDS             complement(26273..27553)
                     /gene="wcaK"
                     /locus_tag="ACUQR0_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HHV9108524.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(27733..29211)
                     /gene="wzxC"
                     /locus_tag="ACUQR0_001417"
     CDS             complement(27733..29211)
                     /gene="wzxC"
                     /locus_tag="ACUQR0_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HHV9108525.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29213..30607)
                     /gene="wcaJ"
                     /locus_tag="ACUQR0_001418"
     CDS             complement(29213..30607)
                     /gene="wcaJ"
                     /locus_tag="ACUQR0_001418"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HHV9108526.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(30662..32032)
                     /gene="cpsG"
                     /locus_tag="ACUQR0_001419"
                     /gene_synonym="manB"
     CDS             complement(30662..32032)
                     /gene="cpsG"
                     /locus_tag="ACUQR0_001419"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HHV9108527.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32225..33661)
                     /gene="cpsB"
                     /locus_tag="ACUQR0_001420"
     CDS             complement(32225..33661)
                     /gene="cpsB"
                     /locus_tag="ACUQR0_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HHV9108528.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(33664..34887)
                     /gene="wcaI"
                     /locus_tag="ACUQR0_001421"
     CDS             complement(33664..34887)
                     /gene="wcaI"
                     /locus_tag="ACUQR0_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HHV9108529.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(34884..35363)
                     /gene="gmm"
                     /locus_tag="ACUQR0_001422"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(34884..35363)
                     /gene="gmm"
                     /locus_tag="ACUQR0_001422"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HHV9108530.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35363..36321)
                     /gene="fcl"
                     /locus_tag="ACUQR0_001423"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35363..36321)
                     /gene="fcl"
                     /locus_tag="ACUQR0_001423"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0050577 - GDP-L-fucose synthase activity
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36324..37445)
                     /gene="gmd"
                     /locus_tag="ACUQR0_001424"
     CDS             complement(36324..37445)
                     /gene="gmd"
                     /locus_tag="ACUQR0_001424"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HHV9108531.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37471..38019)
                     /gene="wcaF"
                     /locus_tag="ACUQR0_001425"
     CDS             complement(37471..38019)
                     /gene="wcaF"
                     /locus_tag="ACUQR0_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HHV9108532.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38035..>38156)
                     /locus_tag="ACUQR0_001426"
     CDS             complement(38035..>38156)
                     /locus_tag="ACUQR0_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HHV9108533.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10187 a         9570 c         8604 g         9795 t
ORIGIN      
        1 tgaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcggttg
       61 ttaacggttc agacaggttt aaagaggaat aagaaaatga cagacaacac tcgtttacgc
      121 atagctatgc agaaatccgg ccgtttaagt gatgactcac gcgaattact ggcgcgctgt
      181 ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa catgccgatt
      241 gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg taatggacgg cgtggtagac
      301 cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg cgcccagggt
      361 gaagatccgc gctactttac cctgcgtcgt ctggatttcg gcggctgccg cctttcgctg
      421 gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct taaacggtaa acgtatcgcc
      481 acctcttatc ctcacctgct caagcgttat ctcgaccaga aaggtatctc ttttaaatcc
      541 tgcttactga acggttctgt cgaagttgcg ccgcgtgccg ggctggcgga tgcgatttgc
      601 gatctggttt ccaccggtgc cacgctggaa gctaacggcc tgcgcgaagt cgaagttatc
      661 taccgctcga aagcctgcct gatccagcgc gatggcgaaa tggaagaatc taaacagcaa
      721 ctgatcgaca agctactgac ccgtattcag ggtgtgatcc aggcgcgcga atcaaaatac
      781 atcatgatgc acgcaccgac cgaacgcctg gatgaagtca tcgccctgct gccaggtgcc
      841 gaacgcccaa ccattctgcc gctggcgggt gatcaacagc gcgtagcgat gcacatggtg
      901 agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga aagcactggg tgccagttca
      961 atcctggtcc tgccgattga gaagatgatg gagtgatcgc catgagcttt aacacaatca
     1021 ttgactggaa tagctgtact gcggagcaac aacgccagct gttaatgcgc ccggcgatct
     1081 ccgcttctga aagcattacc cgcaccgtta acgatattct cgataacgtg aaaacgcgtg
     1141 gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa aaccacggtt accgcgctga
     1201 aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct gagcgacgag ctaaaacagg
     1261 cgatggcggt ggcagtaaag agtattgaaa ccttccacac tgcgcaaaaa ctgccgccgg
     1321 tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt cacgcgcccg gtggcttcag
     1381 tgggtttgta tattccgggc ggctccgccc cgctcttctc aacggtatta atgctggcaa
     1441 ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg ctcaccgccg ccgattgccg
     1501 atgaaattct ttatgcggcg cagctgtgcg atgtgcagga cgtgtttaac gtcggcggcg
     1561 cacaggccat tgccgcgctg gcgtttggta cggaatctgt gccgaaagtg gacaaaatct
     1621 tcgggccggg taacgccttt gtcaccgaag caaaacgcca ggtaagccag cgtctggacg
     1681 gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct ggtgattgct gacagcggcg
     1741 ctacgccgga ttttgtggct tctgatttgc tttctcaggc tgaacacggc cctgactcac
     1801 aggtaatttt actgaccccc gatgctgata tggcgcgtcg cgttgccgag gccgtcgaac
     1861 gccaactggc agaactgccg cgtgccgaaa ccacccgcca ggcactgaac gccagccgcc
     1921 tgatcgtgac taaagattta gcgcagtgcg tggagatctc caaccagtac ggcccggagc
     1981 acctgatcat tcagacccgc aacgcccgcg aactggtcga tggcatcacc agcgccggtt
     2041 cggtatttct tggtgactgg tcaccggaat cggcaggcga ctacgcctcc ggcaccaacc
     2101 acgtgctgcc gacttacggt tacaccgcca cctgttccag cctcgggctg gcagatttcc
     2161 agaaacgcat gaccgtacag gaactgtcga aagaaggttt ctccgcgctg gcttcgacca
     2221 ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa aaatgccgtt actttgcgtg
     2281 ttaacgtcct taaggagcaa gcatgagcac cgtgactatt actgatttag cgcgtgaaaa
     2341 cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg ggcggtaacg gcgacgtctg
     2401 gctgaacgcc aacgaatatc ccacagccgt ggagtttaag cttactcagc aaacgctcaa
     2461 ccgctacccg gaatgccagc caaaagcggt gattgaaaat tacgcgcaat atgcaggcgt
     2521 aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac tgctgatccg
     2581 cgctttttgc gaaccgggta aagacgccat cctctactgc ccgccaacgt acggcatgta
     2641 cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca gtgccgacgc tggaaaactg
     2701 gcaactggac ttacagggca tttccgacaa gctggacggc gtaaaagtgg tctatgtttg
     2761 cagccccaac aaccccaccg ggcaactgat caatccgcag gattttcgca ccctgctgga
     2821 gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct tatatcgagt tttgcccgca
     2881 ggcatcgtta gctggctggc tggcggaata tccgcacctg gcgattttgc gtaccctttc
     2941 gaaagccttc gcgttggcgg ggcttcgttg cggatttacg ctggcaaacg aagaagtcat
     3001 caacctgctg atgaaagtga tcccccccta cccgctctcg acgccggttg ccgacattgc
     3061 cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc gaacgggtgg cgcaaattat
     3121 tacagaacgc gaatacctga ttgccgcact gaaagagatc ccctgcgtgg agcaggtttt
     3181 cgactctgaa accaactaca ttctggcgcg ctttaaagcc tccagtgcag tgtttaaatc
     3241 tttgtgggat cagggcatta tcttacgtga tcagaataaa caaccctctt taagcggctg
     3301 cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc
     3361 ggagcaagtt tgatgagtca gaagtatctt tttatcgatc gcgatggaac cctgattagc
     3421 gaaccgccga gtgattttca ggtggaccgt tttgacaaac tcgcctttga accgggcgtg
     3481 atcccggaat tgctgaagct gcaaaaagcg ggctacaagc tggtgatgat cactaatcag
     3541 gatggtctgg gaacacaaag tttcccgcaa gcggattttg atggcccgca caacctgatg
     3601 atgcagatct tcacctcgca aggcgtgcag tttgatgaag tgctgatttg tccgcacctg
     3661 cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac tggtggaggg ttatctggct
     3721 gagcaagcga tggatcgtgc caacagttat gtaattggcg atcgcgcgac cgacattcaa
     3781 ctggcggaaa gcatgggcat taatggttta cgctacgacc gcgaaaccct gaactggcca
     3841 atgattggcg agcaactcac taaacgagac cgttacgccc atgtagtgcg caacaccaaa
     3901 gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag gtggcagcaa gattaacacc
     3961 ggcgttggct tctttgatca catgctggat cagatcgcca cccacggcgg tttccgcatg
     4021 gaaatcaatg tcaaaggcga cctctatatc gacgatcacc acaccgtcga agataccggc
     4081 ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca aacgcggtat ttgccgcttt
     4141 ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg cactggatat ctctggtcgc
     4201 ccgcacctgg aatataaagc cgagtttacc taccagcgtg tgggcgatct cagcactgaa
     4261 atgatcgaac acttcttccg ttcgctctct tacaccatgg gcgtgacact acacctgaaa
     4321 accaaaggta aaaacgatca tcaccgtgta gaaagcctgt tcaaagcctt tggtcgtacc
     4381 ctgcgccagg ccatccgcgt cgaaggcgac accctgccct cgtcgaaagg agtgctgtaa
     4441 tgaacgtggt gatccttgat actggctgcg ccaacctgaa ctctgtgaag tctgccattg
     4501 cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga cattgtgttg ctggccgata
     4561 aactgttttt acccggcgtc ggcaccgcac aagcagcgat ggatcaggtg cgtgagcgcg
     4621 agctgtttga tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc ttagggatgc
     4681 aactcctggg acgacgcagc gaagagagca atggcgtcga cttgctgggc atcatcgacg
     4741 aagacgtgcc gaaaatgacc gactttggtc tgccactgcc acatatgggc tggaaccgcg
     4801 tttatccgca ggcaggcaac cgcctgtttc aggggattga agacggcgcg tacttttact
     4861 ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat cgcccagtgt aattacggcg
     4921 aaccgttcac cgcggcggta caaaaagata acttctacgg cgtgcagttc cacccggagc
     4981 gttctggtgc cgctggcgct aagttgctga aaaacttcct ggagatgtga tgattattcc
     5041 ggcattagat ttaatcgacg gcaccgtggt gcgtctccat cagggcgatt acggcaaaca
     5101 acgcgattac ggtaacgacc cgctgccgcg cttacaggat tacgctgcgc aaggtgccga
     5161 agtgttgcac ctggtggatc tgaccggggc aaaagatccg gctaagcgtc aaatcccgct
     5221 gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag gttggcggcg gtgttcgtag
     5281 tgaagaagac gtggcggcgt tactggaagc tggcgttgcg cgcgtggtgg tcggttccac
     5341 cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa cgtttcggtg ccgatgcgct
     5401 ggtgctggca ctggatgtcc gtattgacga gcaaggcaac aagcaagtgg cagtcagcgg
     5461 ctggcaagag aactcgggcg tttcactgga acaactggtg gaaacctatc tgcccgtcgg
     5521 cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc acgctggcgg gatctaacgt
     5581 ctctttatat gaagaagtgt gtgccagata tccgcaagtg gcgtttcagt cctccggcgg
     5641 cattggcgac attaacgatg ttgctgccct acgtggcact ggcgtgcgcg gcgtaatagt
     5701 tggtcgtgca ttactggaag gtaaattcac cgtgaaggag gccatcgcat gctggcaaaa
     5761 cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt acagtttcgc
     5821 aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac gctacgccga agaaggtgca
     5881 gatgaactgg tgttctacga tatcaccgct tccagcgatg gccgtgtggt agataaaagc
     5941 tgggtatctc gcgtggcgga agtgatcgac attccgtttt gtgtggcggg tgggattaag
     6001 tctctggaag atgccgcgaa aattctttcc tttggcgcgg ataaaatttc catcaactcc
     6061 cctgcgctgg cagacccaac attaattact cgcctagccg atcgctttgg cgtgcagtgt
     6121 attgtggttg gtattgatac ctggtacgac gccgaaaccg gtaaatatca tgtaaatcaa
     6181 tataccggcg atgaaagccg cacccgcgtc actcaatggg aaacgctcga ctgggtagag
     6241 gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca tgatgaatca ggacggcgtg
     6301 tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg aagtttgcca cgtcccgctg
     6361 attgcctccg gtggcgcggg caccatggaa cacttcctgg aagccttccg cgatgccgac
     6421 gttgacggtg cgctggcagc ttccgtattc cacaaacaaa taatcaatat tggcgaatta
     6481 aaagcgtacc tggcaacaca gggcgtggag atcaggatat gttaacagaa caacaacgtc
     6541 gcgaactgga ctgggaaaaa accgacggtc tgatgccggt gattgtgcaa cacgcggtat
     6601 ctggcgaagt gttaatgctg ggctacatga acccggaagc cttagacaaa accatcgaaa
     6661 gcggtaaagt caccttcttc tcgcgcacta aacagcgact gtggaccaaa ggcgaaacct
     6721 cgggcaattt tctcaatgtg gtaagtattg ccccggactg cgacaacgac acattactgg
     6781 tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac cagtagctgc ttcggcaaca
     6841 ccgctcacca gtggctgttc ctgtatcaac tggaacaact gctcgccgaa cgtaaatatg
     6901 ccgacccgga aacctcctac accgccaaac tgtatgccag cggcactaaa cgcattgcgc
     6961 agaaagtggg cgaagaaggc gtggaaaccg cactggcagc aacggtacat gatcgctttg
     7021 agctgaccaa cgaggcatct gacttgatgt atcacctgct ggtgctgttg caggatcagg
     7081 atctggattt aacgacagta attgagaacc tgcataaacg gcatcagtga gttacgggct
     7141 gagcaggtgt gatgttgttg tcacacccgg agccattgcc cgatgcgcaa acttatcgtg
     7201 cctacacatt atctcaacac acgtaggccg gataagatgc gccagcatcg catccggcaa
     7261 aaaaaacggg catggtgtca ccaccctgcc cgtttctctt aaatgcacaa taatattact
     7321 tcgcgttgta attacgcaga gcgttacgcc ccaacacaat ccccgcgcca accatgccgc
     7381 ccagaagcac tgccagaatc aaggtgattg cctttttcgg actatcgcga cgaataggta
     7441 acgtcggttt catcacatag cggtaagcat gaatatcaag atcatcaaac tttaattttt
     7501 caatatccaa caggttttga cgtgtctgat agtagtttgg tgagaacacc aacggacgag
     7561 tcgcttcatg cttaatcatc gactccagcg cttcgctccc tagaaggaac aacgtatctt
     7621 gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga ttcgcatact
     7681 gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgcg accacttcct
     7741 gggttcttaa agagtcctgc aagtttttcc gtcccagagc aatgttgtcc ttgagatcct
     7801 tttctagctc ttgattcact ttatcatcaa cttgctgaat gtattgggcc aacttcattt
     7861 gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa tggtaattgc tggttcttaa
     7921 cagaaggttc gatggtaagt ttttctggct cttcctgatt atccagcgtt tctgctaatg
     7981 cagagaaggc agaactgaag cgaccaatta acgtctcctg caaatccgat actttcggtg
     8041 cagcctgacc atagataaca ttcatggcat tgttatagcc agcaatttgc cccacgtcgg
     8101 gctgagtgat aattgctgtt gacgtccatt tctccttcgc tactgccaaa taaccaatag
     8161 ccagggcaat agccacaatg acggaaatga taattgtcat cttgccacgc cacaactgca
     8221 ctagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa acattattat
     8281 tttctactct catcattacc ctaactgacg gaaatttcca agtccgcata gtttagcgat
     8341 aatccccgtg aatgctatag gataagtgat aaaaataatg aattattgcg gaggtaacaa
     8401 aagacaatca gggcgtaaat agccctgata acaagatgtt agtcgctgcc aaagagatcg
     8461 cgggtgtaga ccttatctgc cacatcctta agctcttctg ccatacggtt ggagataatg
     8521 acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca ggcgagagtt gaagaatgag
     8581 tcttctttca tcaccggctc gtagatgatc acttcaacgc ctttcgcctt gatacgcttc
     8641 ataatcccct gaatagaaga cgcacggaag ttatctgacc cgctcttcat aatcagacga
     8701 taaataccca ccacttgcgg tttgcatcga caattgccga gatcaggtta ttcggcacag
     8761 actggtagtt cgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa
     8821 acgacggatt gttgtaatgg ttgccaatac gcggatcgag acaaacgcct tcgataatct
     8881 ggcgagtatt cagacctaaa ctttctgcat aactatccag ttcattaaag tatgctacgc
     8941 gcatcgccag ataggtatta gcgaaaagtt taatcgcttc tgcttcagtg gagtcggtaa
     9001 acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa caacgcagca aaacgttctg
     9061 cgcgttctga acgctcaccg atgacaatac gtgacggatg gagattatcg taaagggctt
     9121 taccttcgcg gaggaattcc ggagagaaaa taatattttc agtacgatat ttcttatgca
     9181 tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac cataaccgca taaggattta
     9241 tctcaactac gtctttaatt actgattcta cactggatgt attgaaataa ttagttttag
     9301 gatcatagtc ggttggagtg gcgatgatga cataatcagc atcccggtag gcttcatttt
     9361 tatctaatgt ggcattaaag tgtattttat ctgattgcaa aaactgctga atttccttat
     9421 caacaatagg agatatccga tcattcagca tagcaacgcg tgacggtaaa atatctaatg
     9481 ccacaacctc atgattttgt gcgattagaa gcccgtttga caagcctaca tagccagtac
     9541 cggaaatggt gattttcatt tcatgctctc agaattaact taactgtgaa tcatgatgtt
     9601 tttagcatcg tgataagatg ctaaaagttt taacgctacg ttgtcagatg gtcaacgcaa
     9661 cgaaaaacaa ttcagatttt ctttataaga atagctgatg tttattaagt taatattaag
     9721 cagtgaaatt tagtctataa gatatttggc aaaaaaaagc ccggtgcaat atacgccggg
     9781 cctcaatttt attgttggtt aaatcagatt aatccagcca ttcagtatgg aacacacctt
     9841 ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc acgctgtgcc tggatcaggt
     9901 tcgcaggcag aaccgcggca cggtagctat cgtaataggc aaccgcagcg gcgaaggtcg
     9961 gaaccgggat accgttctgt actgcataag cgacgacatc gcgcagcgcc tgctggtagt
    10021 catcggcaat ttgcttgaag tacggggcca gcagcaggtt agcgatctgc ggattttcgg
    10081 cataagcatc ggtgattttc tgcaggaact gcgcacggat gatgcagcca gcacggaaaa
    10141 tcttcgcgat ttcgccgtag ttcagatccc aattgtattc ttcagacgca gcacgcagct
    10201 gagagaagcc ctgagcgtaa gaaacgattt tgcccagata cagcgcactg cgaacttttt
    10261 cgatgaactc agccttgtcg cctgctgact gagcttgcgg accagagaga actttagatg
    10321 cggcaacgcg ctgatctttc agagaagaga tataacgtgc aaacacagac tcggtaatca
    10381 gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt ccatttaccg gtgcctttgt
    10441 ttgctgcttc atccagaatc acatcaacca ggtagttacc gtcttcatct tttttggtga
    10501 agatatcttt agtgatgtcg atcaggtagc tgctaagttc accgttattc cactcggtga
    10561 aagtctgggc cagttcttca ttagatagat tcaggccacc tttgagcaga gaatacgctt
    10621 cagcaatgag ctgcatatct ccgtattcaa taccattgtg aaccatcttc acataatggc
    10681 ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc gtcttctgcc acggcagcga
    10741 ttttagtcag gataggcgca accagctcat aagcctcttt ctgcccgcca ggcataatag
    10801 aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc ggtaccgatg aagttaaagc
    10861 cctctgctga aagctcacga ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat
    10921 caatgatgat gtcaccttta tcgaggtatg gtttcaggga atcaatcgca gcatccgtgc
    10981 ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt ttccagagat tcaacaaact
    11041 ctttcaccgt atagtaagga gccagtttct tgcctggatt ttcagcaatc acttcttccg
    11101 tcttttcgcg ggagcggttg aaaatagaga cggtataacc acgactttcg atgttgagcg
    11161 caaggttgcg ccccatcact gccataccga ctacgccgat ctgttgcttt gacattgttt
    11221 actcctgtct tctatattat taaccgtcta ttaaccgtta tattattaac cgtcgtgatt
    11281 ttcatcttct aatcaaggaa tacatatggt tacagcatgc ttcttacttt atagcgataa
    11341 gtatgccatt gggtgtatcc ataagaactt tgaattttgg atacttttct ggattaaaat
    11401 tagcatagct tcgtttagat ataacaataa acgaactatt aaaagatttc atcttctcta
    11461 ttaaatcatc ctgtgggatt acctcacact cgggattact aatagtccaa tcactatatc
    11521 ttgctaatcc aaagacgtca ccaattgcca tccctttata gaagtaaata ccatcctcca
    11581 agcctaattg gataagatta tctccatata actcactata ttcacttgct ttcttcatca
    11641 gttcatagct atagcgactt tctaagcgtg aattaaaatt caagtattca gaataaaaat
    11701 atttgaattc aaaatacaac gatcgccccg aaaaaaaata atacacacaa ggataaaata
    11761 tcgtcccaac ttgtaatttg taatgttgat atcttttatt aaacttgcaa ttgagatcgc
    11821 aacctgcaat actgacaatg ggtaaatggg tgataaatac ctgtcacctt gcgtaacaga
    11881 aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat acccaaagta ttttattatt
    11941 cctaagtgta aagacagaca gtatagcata aaaaacaaca tcactgccaa ctttattaat
    12001 tgcttgttgc aaatctaatg tggcaggact tataccatgt cgagcttgtt ctgctgtttg
    12061 aacttgtaga tcaatttcat tccataggta gtgaccaaat atatttcctc cagcaggact
    12121 aaaaggatca ccagagatcc aaaaactacg gacataccaa taaatgccac caagaattgt
    12181 tgccaagcta aaaataagaa atgtctgtat gtttctcttt ttcgctatca atataacaaa
    12241 tatgatggct ccatatatgg caccaaatag ttttacgcca caagcaattc caataaaaaa
    12301 accacataaa aaaattaatt cattttgtgt tttattgttt tctgagcgaa tatatataag
    12361 aatactggct gcagcaaaac aaagatagcg ttaaatcaat atatgcataa ccaagatgat
    12421 ctttaagtgg attgatagta aatagtagga ttgtagcaat aatgcctgga taataagttt
    12481 tcgtaatttc ccatgaacag ccaacaagtc cacaggctat tacaaaaaaa cagatatttg
    12541 ctaaaaactg agctaacctt acatctccta actgtaaacc taaagccatt aaaagatctg
    12601 cattttgcgg gaacagagga aaacgcaaat attgttcaac aacaattgca tggtgctcaa
    12661 ctaatgaacg agccaaaggt aagtgataca tagtatcatc ccaatcaccg ggagctcgca
    12721 gggacgataa caatgtaacc acaaaaatca aaacaagaaa taaaataaca aacagcgtac
    12781 tgaactcaat attatttatt gccagccatc taaattgctg acgtttaata atgcttaaaa
    12841 cccaacacac cgtaaaaatt aaaaagatag caatcttatt aaattgcccg accatgccca
    12901 gaaagtaaat tatcaaaata tttaaagaca tccctatgca taggcaataa aaaataaaga
    12961 aaatttgtta agtcctgcca ccccctcctt aaaaaaagat gacaatacaa tattaccaat
    13021 gacaaaaaat gtcattccaa aagcaaaaca atacacaaca tacaaataca acggcaattc
    13081 aatcatagta aaatactcta tttacattat cagtttatag tttttttcag aaagataaaa
    13141 taatgccttc atttcacgtc tagttgttgt atttgaatgc aaaataatgc cacatacaaa
    13201 tgccattatt gacgataaca tgatggaggc agttaaaaag gcagtcggaa atcttctcac
    13261 caaacctgta tcaatataat caacaatgat gggcaatcct aaaattatag acaaagcagc
    13321 aagtaaaacg ccgataatac taaaaaagta tagtggccgt tcaagcgagt aaagtttaat
    13381 tattgttttc agaatcttga acccatcact ccaagttgat agtttgctca cactcccttc
    13441 agatcgctcg ccatattttg tattaacctc accatacttc atacgtaatt ctaaagcatg
    13501 aattgtaagc tcagtttcga tttcaaatcc ccttgataga catggaaaag atttaacata
    13561 gcgtctggaa aatacacgat accccgacaa catatcagta aaattccctt taaagatagt
    13621 ctgaacagta ccagtcaaca gtttatttcc tcagcgatgc ccttttctgt atgtattttg
    13681 atctccattc tcttgacgac atcctacaac catatccaat tgatttttta tgagatggtt
    13741 aatcatttct ggagcagaac ttgcatcata agtgtcatct ccatcaacca ttagataaat
    13801 atccgcatct acatctgaga acatacgtct tacgacattc cccttgcctt tcagaggaac
    13861 agagtgaact gttgctccag catcctctgc cacttgtgct gtactgtcag tcgaattatt
    13921 atcaaataca tatatagaag cagttggaat attcgttttg aaatcattta tgacttttgc
    13981 tattgccttc tgctcattat aacaaggaat aattacagcg atattaaaag agtgatacat
    14041 aagcccctca tataaactat ttagctaatg tgcaagtaat tgtgttttta acaaatcgtt
    14101 tactaacgaa aaatgaaata ttttgatgcg caatatccta aaaccaaact aaaaagagaa
    14161 aaggtgaaca atgtaaatat tggagataaa gctaagagat caaataaaac gccgaataaa
    14221 taactcatcg cccccataaa aaatataaat ataaaatatc tagccttggt tgcagatact
    14281 ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg ctgcacatat aaaaccaaca
    14341 atgttacttc ggccttgacc aaaagctaaa ttattataac acgcataaaa taccccccaa
    14401 tgtaaagcag tattgacaat tccaattaaa aaataactaa aaaaagaact ggtcaataac
    14461 ttccctattt ttaacatcct ttattttgct ccagaagtga ggttattact aatttggata
    14521 ttttctatag aaaataccca taaaactaca acgcctataa taaaaattcc catgggtgta
    14581 aaaagaaatg gattagttat agaaacggta atactgaaaa aaagtattaa cgacatcatt
    14641 aatgatgttt ttgtactatt taaaaaatag cctttcaaaa agataagcaa cataggaact
    14701 aatgatataa atacaccaat aacgcctgtt ttctgaagta tctcaagagg tactatttca
    14761 atccttaatc gatctaatat aaatgaacca aatcctcttc caaacaagaa cgtcgctaaa
    14821 tcaacatttt tataataaaa atataaatca ttaaatctaa cagagtcaga atcttcactg
    14881 cgtgatccga ccatgtaaag cataattata aatagaacgc tgcccaatat tattataata
    14941 gtatttaaat atagcttatg taatttaaaa ctggctaata acagtgataa tgttgtaaaa
    15001 agtaatacac ctcttgattc tgtcaataat atcgcaagca ctattatgag acattttaaa
    15061 tattttttgt cgacaaaact gataatcgca ccgacaccaa aaaagtagaa ggatttggaa
    15121 aaaaaagccc catcgggacg aaaaaagaac tcgctagtca atgataagtg ttcataaatt
    15181 aaattaaaat ttaacaaccc gaaattcaac aaaattatgt atgaaatata taacagtgat
    15241 ccatataatg caaatgcatt aactatttta ataaaaatct cacatgaaaa tacttttaaa
    15301 gtatatctta gtgtaaatag atatataagt gacagcataa aaaggtatgg ttgcacatca
    15361 aacagtatat catttatttt attaccagct aataagccga caaagaagcc ataaaaagga
    15421 aataaaagca accatataaa aagactgaca catttttggg tgattgaaaa attgaatgta
    15481 actaaattaa aaattaaaag tataaaacta aatacaaata ataaatatcg caaagggaat
    15541 attcccaatg gctccagtaa tcttccacca ccaccaataa tcagttcaat acataaaaat
    15601 gttataaaaa ttttatttat attattcata ttcgtaaggt gatgtttttt aaaatatgtt
    15661 taaaatcaac attaataaaa tataaatttt atcagccttt ttccgcctta acttccaaaa
    15721 acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat tcacaaagct
    15781 atcatcagct aaggcattag ctattttagt tacttctttt cgataccact tgtttctcac
    15841 caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt ggttgttttc
    15901 atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat ttctcgtccg
    15961 cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat ttttattatt
    16021 tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg tacaccccgg
    16081 acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat caactaaatt
    16141 ttttcgccct gatggataat atgcaattac attgctggaa taacaattcg cattattaaa
    16201 taccaagcag tcaattgcat tttttatttt atctttatac caaatatcgt cctgatcact
    16261 aaaacagata taatcatagt tttcaagatc gacatcttta attaatctat aaaaattttt
    16321 acctgccccc ccaaacttat tgacgcttgg aagtatattt ataattgagg gatatgataa
    16381 ttgaaattct tcacaaatat ttaatgtatt atcagttgat aaatcatctg atataaaaat
    16441 tgagatgtct acatcttttt gttcaataat actttttaat tgttcctcaa tccagcattc
    16501 gccattatac gtagccataa gtacagctac tcttttttga tcagaactat gcattataac
    16561 gaccgccccc agtaattcct cttattccat ccagcgtagc caaaatatgt tttttcatat
    16621 attcaatttt atttttttga gttgcgataa ttaaagtcgt atgaattaaa gaaaaaacaa
    16681 cttccctaat agagagtaaa agaggcacat gattttttct aagcaaaaga aatgcatttc
    16741 ttactcgata ataacgacgt actggcgagt gaattggtat gttaatccca cagattttta
    16801 ctgacttatc cccaatagca tgaaccattt ctatatcagg aatgatatga actaatatgc
    16861 catagtttaa gcatctaaga caccattctg tatcgacata atcaataaaa agttcctcat
    16921 ccatcattcc aacaatctca aaaacttctt ttgaaaccat ggttccggat gagattgtta
    16981 ctgaactttt aaacggtgtc tgtccttcag tgacatgaat tttctctcga agaccatttt
    17041 ttttgatatt acaaatagga taataatgtg acttatctct atcaataaaa actgggccag
    17101 ctatttttgc attttcatta tccatatagc aggaaagttt tttgataaac tcattgcgaa
    17161 tacggctatc ttgatcaaaa aaaattatct tctctgcccc tttttcaaga gaatatcgaa
    17221 tcccaatatt ttgtgctttt gctatacctt cattcctacc aagacaaatt aaatgaactt
    17281 tttcaatgtc tgctaattta tctttcaagg tgaaattagt cctattatcg actactacaa
    17341 cgtaacaatt ttgctccttt agttcagtga tcagtgcatt cagattttta agttcgggat
    17401 tatatgtcac aatgacagcg taaatattac tattcattat ccaagtgact cagtaattgg
    17461 ttaattttag ttttcatata cagaacacat tctagtataa agccattgta ataacagagc
    17521 atctaaaatt acacgtatgc tccaggatat tgcggcaccc agtaacccat atttttcgac
    17581 cataaaatac aaaagggcta aataaggaac caattcagca caatgaatca aagcagtaat
    17641 tttagatttt cctaatgcct ggattgcaga gaatggtatc attgccagcg cattgaagaa
    17701 aaaaccgatc aataacacat ttaatatatt aaaggaatta attttataat tctcaccaaa
    17761 ccatattttc aatacaagcc ctgaatataa caagcctatg attatcaccg gcaaacagat
    17821 tagaaacatt aaaagcaatg atttatttac attacgttta aattctttaa agtctttgat
    17881 gttacttaac cttggaaaca ctgccctccc gattgctgca ggtattattc ctaattttag
    17941 aataacctcc gctggtgcag aataaaatgc aactttatct gcccccataa tatttgacac
    18001 tataaatcga tcaaaataca ccatgaccgg acttatgata ttacttactg tcatccaacc
    18061 gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa tgaacccccg aaattttaat
    18121 atcattcctg acataatagg cagagactaa aatcgaaata actcttgcaa atattaaacc
    18181 agcaaccgct gccgacaatg tagcactata aaaaacaaat atggccggaa ttcccgcgat
    18241 gcaagagctt gatatagatt tttgaatatt tacaatgcca aatttttcat ccccctcaag
    18301 aatggctgac cataattgat ttagaataaa taatggaatg caaatagcca acagtttaaa
    18361 tgctagttgt atatcactat gttcaacacc agaaattttt agataattaa caattccatc
    18421 agagaaaatc aataataaaa aagcaccaaa gcatgaaaat agcaccaaaa aagatgtact
    18481 tgttgaaatt accttggttc tttcatgatg attatcacga tgaattgcaa tttctctaat
    18541 aacagagcga gtcaggccta catcaaaaat tccagcatac ccaacaagtg caattgcaat
    18601 tgtataaata ccgaaacctt caggccccaa acctcgagcg agaaatccta gcgaggggat
    18661 agcaattaaa gttggtattg catagccaaa aaggttccag acactatttt ttattatgct
    18721 cattattaca tttctttatc atataaaata atcagcatct acaaattttt gaccattaat
    18781 atctttatca gaaagcaatg gattttgaac cggccattct atatttatac ttttgtcgct
    18841 ccataaaatc ccacgctctt gcatttttga atagaaatta tttgttttgt agacaaattc
    18901 tgcttcatca ctaagaacgc aaaacccatg agcgaatcct tccggtatcc ataattgacg
    18961 cttattctcc gccgacaaat ttacaccaac ccacttgcca aatgttggtg atgatttccg
    19021 aatatcaaca gctacgtcaa aaacttcacc gacaacacag cgtaccagtt ttccttgagc
    19081 ataaggttcc agctgatagt gcagtccgcg taacacaccc ttagttgatt tagaatggtt
    19141 atcctgaaca aactcaacct ttcgtcctac agcctcttcg aaaactttct gattgaagct
    19201 ttccataaaa aagccccgct catcaccaaa tacttttggt tcaaaaatta gcacatcagg
    19261 aatttcagtt ttaattacgt tcattttatt aataaccttt aatcattttc agcagatact
    19321 gaccataagc atttttcttc agcggctcgg ctaatacctt cacttgctca gcatcaataa
    19381 agcctttacg gtaagcaatt tcttccgggc aggaaacttt cagcccctga cgctcttcaa
    19441 ttgttgcaat gaagttgctt gcttcaataa gactttgatg tgtccccgtg tccagccatg
    19501 cataaccacg ccccatcata gcgacagaca aacgtccttg gtccatataa atacggttaa
    19561 tatcggtaat ttccagttcg ccacgtgcag atggtttaag gtttttcgcc atttctacaa
    19621 cgtcattgtc atagaaataa agcccagtaa ccgcatagtt actttttggt tccagcggtt
    19681 tttcttccag actaattgcc gtaccgttat catcaaattc caccacaccg tagcgctcag
    19741 gatcatttac gtgataggca aaaaccgttg cgccactctc tctgttaaca gcggtatcca
    19801 ttaatttcgg caggtcgtga ccatagaaga tattatcacc aagtaccagc gcacaatcat
    19861 caccacctat aaattcttca ccaatgataa atgcttgcgc cagaccatcc ggactcggtt
    19921 gtactttata ttgcagatta agcccccact ggcttccatc accaagcaat tgttgaaaac
    19981 gtggtgtatc ctgcggcgta ctgataatca gaatatctcg aatacccgcc aacataagcg
    20041 ttgaaagcgg ataataaatc atcggtttat cataaatcgg tagcagttgt ttgctcacag
    20101 ccattgtcac aggataaaga cgagtgccgg aaccaccagc cagaataata cctttacgcg
    20161 ttttcatttc accattcctt ttaattcatc tcgctctagc atcatgagcg agatgcaata
    20221 atttgttaaa ttgccgtggt cgtaaataat tcgttgagca tacgtttcac gcccacctgc
    20281 cagtcaggca agacaagcgc aaagttctgc tgaaattttt cggtattgag gcgagaatta
    20341 tggggacgac gtgctggtgt gggataggcc gttgtcggca cggcattaag tttgttaaga
    20401 gcaaggttga tccccgctct gcgcgcttct tcaaatacca gagcggcata atcgtgccag
    20461 gttgttgtac caccagcaac cagatggtac aaaccagcaa cttctggttt atttgcggcc
    20521 acgcgaatgg catgagcggt gcaatcagcc agtaattcag cccccgttgg tgcgccgaat
    20581 tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca ggcgcaacat cgttttggca
    20641 aagttatttc ctttaccagc gtatacccag ctggtacgga aaataagatg ctttgcgcaa
    20701 tgttcttgta acgctttttc cccagctagc ttggtttcac cgtagacatt tagcggtgct
    20761 gttgcatccg tttccagcca tggcgtgtca ccgttgcctg ggaaaacata atcagtggag
    20821 tagtgtataa cccaggctcc gacttcatta gctgcttttg caatcgcttc gacgcatgtc
    20881 gcgttaagta attgcgcgaa attcggttct gattccgctt tgtctactgc agtgtgagca
    20941 gcagcattaa caataacatc ggggcgaatt tttttgacgg tttcagccac accttcgggg
    21001 ttgctgaaat ctccacaata atcagtggag tgaacatcga gcgcaatcaa gttacccaga
    21061 ggagccagag cacgctgcag ttcccaaccc acctgccctg ttttgccgaa aagcaggata
    21121 ttcattactg acggccctca tagttttgtt caatccacga ctgataagca ccgcttttca
    21181 cattctcaac ccaatttgta ttagccagat accattccac cgttttacga atcccgctct
    21241 caaacgtttc ttgcggtttc cagcccaatt cgcggctaat tttatcggca tcaatggcgt
    21301 aacggcggtc atgccccggg cgatcggcaa cataagtaat ttgctcacga taagatttct
    21361 ctttcggtac aatctcatcc aacaaatcac aaatagtgag cactacgtcg atgtttttct
    21421 tttcgttatg tccaccaatg ttataagttt cacccgcttt accttcggtt acgacggtat
    21481 ataacgcacg agcatgatcc tctacataca accagtcgcg aatttgattc cctttaccat
    21541 aaataggtaa tgccttacct tccagagcat tcagaataac caatggaata agtttttccg
    21601 ggaagtgata aggaccataa ttgttcgagc aattagtcac aatggttggt aaaccataag
    21661 tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt agacgcagaa tacgggctac
    21721 ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc ttcgttatta tttgcttcat
    21781 ccggatgggg taagtcacca taaacttcat cagtagaaat atgatgaaag cggaaacttt
    21841 ttttcttttc atcattcaga gcagaccagt aattgcgcgc cgcttctaaa agaacataag
    21901 tccccacaat attggtttca ataaatgccg cagggcccgt tattgaacgg tcaacgtggc
    21961 tctctgccgc caggtgcatc accgcgtctg gctggtgctg tgcgaaaata cgcgacatgg
    22021 ctacagcatc gcagatatct gcatgctcaa aagcataacg ttcagaatca gaaacatcag
    22081 cgagtgattc caggttcccg gcgtatgtta atttatcgac attaacaaca ctatcttgcg
    22141 tattatttat tatgtgacga acaacagcag aaccaataaa ccctgcgcca ccagtaacaa
    22201 gtatcttcac ttttttattc catatagcta tagagcatgc tgtgaaatag actgctccag
    22261 atttgattaa tagaggtatt aatacacgct accgcccctg gcttaacagc taccagtgca
    22321 ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg cgccaaatcc gaaaaattca
    22381 aacgttaatt gtcttaccaa acagccgtag aaacaaggaa aatcctggaa aaatttgaat
    22441 aatatgagct atgtaacttc ttgtttttat aggctttata aaaacggcaa cggcaggaaa
    22501 attcaatgag caaaacttat gccacgaatc ttcactgccg ttataatttt cttatcaacc
    22561 gttacatccg gtcagatttt cattattcgc ttaacagctt ctcaatacct ttacggaact
    22621 tcgccccttc tttcaggttg cgtaggccat acttcacaaa cgcctgcata tagcccattt
    22681 ttttgccgca gtcgtaactg tcgccggtca tcagcattgc atcaacggat tgttttttcg
    22741 ccagctcggc aatagcatca gtcagctgaa tacgtcccca tgcaccaggc tgagtacgtt
    22801 ccagttccgg ccaaatatcg gcagaaagca catagcgacc tacggccatg atgtctgagt
    22861 ccagcgtctg cggctgatcc ggtttttcga taaattcaac aatgcggctg actttaccct
    22921 cacggtccag cggctcttta gtctggatga cggagtattc agagaggtca cccggcatac
    22981 gttttgccag cacctggctg cggcccgttt cgttgaaacg tgcaatcatg gcagcaaggt
    23041 tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac gtctggcagt accacgacaa
    23101 atgggttgtc accaatggca ggtcgcgcac acaaaatgga gtggcctaaa cctaaaggtt
    23161 cgccctgacg cacgttcata atgatcacgc ccggcggaca gatggactgt acttccgcca
    23221 gcagttgacg cttcacgcgc tgctcaagga gtgattctaa ctcataagag gtgtcgaagt
    23281 ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag gatttctttg atccctgcag
    23341 ccacaatctc gtcaacaatg tactgaatca ttggcttgtc gacgattggt agcatctctt
    23401 tgggtatcgc cttagtggca ggcaacatat gcatcccgag acccgctaca ggaataactg
    23461 cttttaaatt cgtcattatt tcatccacct gtaaaatggt tgctgaatta tagcttgttc
    23521 gatttttttc gccagcatca attaccctga attgattact gaattacttg tgatgttacg
    23581 ccgcttcgtt gtggattgca gtagcattgt tcctaagtat ggctccattt ttccaggaat
    23641 ggtcgcaaat ctactccctc cgttccggca atctaaagtt aatcttttcc acattaacaa
    23701 tatggtgatt aatcctgtcg atatcgacgg agctttgccc tctttcattc accgcatgaa
    23761 catttgcaag agacagcagt gtttcttttt tcgccataaa gacgccgcga acgtctttgc
    23821 gcatgtcgaa gttcatgctc aatgctggtc caactgagga ttcctgcatc acattgatat
    23881 ttcgcataaa aagatgttgc ggcttgttgt gtaactccag cgaggcacgc ttcatctcaa
    23941 tgttagttag cgacacaaag gagacggcat tcccggcgga gatttggatg ccgcgcaatt
    24001 tataagcaag atgggtatta tccagttgaa tattattcac tcggaaattt tgcggtatcg
    24061 agagatattt gcctttaatt accccatagc cgattaacat cccggcgcta ttaatcattt
    24121 caatattatc aatcacgaaa ttgtcacaac cgtaaatagc gactgtcgcg ttatcaatgc
    24181 ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt gatattacga ataacaaaat
    24241 gtttaccatt ttcaacatgg atcaactgcc gacaatccga tcccgtgata ttcgccacga
    24301 caaagttttt cactgcctgg tcttccgggt agttgttatc ataagtgctt cccgcaaggc
    24361 ctatgccgat gccccagttg attttgccgt tggtacagtt gatacgctcg atgacatggt
    24421 cagatatcaa aatatcactg tcgttaattg ccacgttcca ttcaatggcg tcgccctgta
    24481 agtcgctgaa cttacagttg gtaatgttgg caccgataat ctggttatga aatccctggc
    24541 gtaagatggc gtaattagcg tggctgacag tgaggttatc gatggtcagg ttgcgcatga
    24601 cccgtttgtt tttgccgcca atataaatct gcattaccgg gccaaagccg ctcatcgcca
    24661 gccctttgat ggtgcagtca gaacaacgca catccagggt aatgttatgc ataccgccgc
    24721 cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa tcgccctctg ccgttgccgc
    24781 gcaggcttcc aaggatgtgt aacgttttgc cgggagggat gaagatgccg gtgttgatat
    24841 tgtcacaaac gaatccggca ggcacgacga ccgtttgccc ttcgctgaag gcttgtttaa
    24901 atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt aacgctttgt cgggcgggaa
    24961 gtgcgcgagc gaaaggggta tggaggaagg caagcgccga gcttgccgtc aggaaggtgc
    25021 gtcgggagag tgttttaaat ggcatacgtt ctcctctata aagcctgcag caagctggcg
    25081 agttctcgat taatcacctg ctggttaaaa tcgtgttcga ctttttcgcg cgcacgtttg
    25141 acgaccggag ccagttcgtc ggtatccagt tggctaaacg ccgccagacg ttgtgccagt
    25201 gcgcgagcat cgttctcagg cagcagccag ccggatttat cggcctccac cagttcagga
    25261 attccgctat gcagagtaga aaccaccgga ataccgaccg ccatcgcttc cattagcgcc
    25321 accggaatgc cttccatatc gccatccgcg cccgttaccg atggcaacag gaagacatcc
    25381 gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa agcccggcat ctctaccaca
    25441 tcttccagtt gatattgttc gatgagggtg cgcaggcgtc gttcccacag gccaatgccg
    25501 aggatgcgat agcgaaatgc cacgccctgc tctttcaact gacggcaggc ttcaatcgcc
    25561 acatgcaggc cttttttctc ggttaagcgt gcgacagata tgatttccag cggcgttgcg
    25621 ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca cgcccatacg cgatacggcg
    25681 attttttctc tggggcagcc cattttttgc agccttccgg cccacagatc gcttatcggt
    25741 aacatcaggt cgccacggcg aaacagttgt tgatattcgg gagtgtagtg gttgagcact
    25801 tcccgactgg agatatcaat accgtggaag atggtggcaa ttttgccgcg aatgataccc
    25861 agttcgcgta gttttgctgc ggttaccccc gctgggccaa aatgagcgat gaacacatcg
    25921 gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca aaatcaggtt ccgcgactcg
    25981 gcaccatagc gtttgaggtt gagcgcatgc caggtatttt tacgatgaat gccacgtaag
    26041 gtctggctgg cgcggtggcg cagtttcgct actttgcccg caggttcgtc ctgtaaccag
    26101 cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt gggtgttctc tgtatcgcct
    26161 ttttgcaaag cgacaatctc cacttcaaag cccatatcaa taaacgcggt aatttgattg
    26221 aggacgaagg tttccgacga cagcgggaat ttcaataaga agaagccgac cttcatttca
    26281 cctccccgat gcgctcaagc acagactgca ccatctgcat tcccgtctga cgttcgcgac
    26341 tgacggcttc gttaaatcga gcgttcagca ccggaagctg gcctaaggta tccgcaacca
    26401 tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat cgccatctct ggcagtccca
    26461 gctgctgcat aatcccggcg gatttatgtt catagttgat ggcaatcgcc ggagtagcaa
    26521 agttcattga gataatggcg gagtgcaggc gcgtaccgac ggtgagttca caggctccga
    26581 gaattttgcc catttccaga tcgttgagtt catccatcac tacgtggtaa cgggcaggat
    26641 cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc gtctttgtta tagctgtcga
    26701 tacccgtaca ggtggagagc gcaatcacct gatagccttc atcgagaatg cgattgacca
    26761 ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc gagacgtttg tcgaacggtg
    26821 ccagttcgcg cagggtaatt gccactgttt tctgttgtgc ggcaacgtcc agccagtgtt
    26881 gaacggcata gctggcggtg aagtcttcgg tgtggtgatc gaccagccac gcggtatcga
    26941 cgccatgttc cacttttgcg gtggtgatat ttctgcgttt catcaaatca aggctgaccg
    27001 attcgcgcag gatcagcgcg tcgcagtgac caaagacata gttcgccagt tggttaaatt
    27061 gctcatcctg gaaggggccg acgctgtgac caatcataaa cagcggcttt ttcgccataa
    27121 atgtgcaaag cgcatgttca aactgcggca cgccgtagag atcgacaaaa aacgatccgc
    27181 cgacctgaat aatggcgtcg taacctgaca gcaggcgcac gaagtcggtg aatccctggg
    27241 cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg tgagagcaat acctgatgct
    27301 ggtagcggcg acggaggact tttttaatgc gcccgacaac gcccgccgcg ctattgtgtt
    27361 gtgtcatctg caggaacagc ggatcgccca ttaccgggcg gttgagcagc caggaagaac
    27421 tgaccggata gcggctcatc acgtccactt cggtatgtgg attgaggatg ttgatggcat
    27481 caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc gcaagtgtgg ttgcccagaa
    27541 taagtaattt cattttttcc tcataaattt gatgccaggt gaggctgtgt ttgttgtgtt
    27601 cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta tcaggcctac aggtcccgag
    27661 cacagaaccg taggacggat aaggcgtttt acgccgcatc cggcaaccgt tgtcggaacc
    27721 gaaaacagca attcaccccg cccgtaaaag cattttcatt ttttcgctgc gacaaaactg
    27781 acgcttcact tccaccacca gcggatggcg tgacagcaca atcatcacca caaacgccag
    27841 cacgcccgcg gctatttgca ccgccagcag catccccagc gccagttgcc ctttcagcac
    27901 aatgcccagc gcataactga ccaccagcgt cggcaacgag agataaaacg gcagccacaa
    27961 actcaggatg tactggcgat aactggaacc aagaaccggt ttaatcatca cgaagtaact
    28021 cagaatggtg ttgataatct gcaccagcag gaaaccgagc gtgacgccga ttgcgcccgc
    28081 catctgccca ccaataacaa tcgccggaat aaacagaaat gttttgaata cgttgaattt
    28141 aaagctgata tcgacccgcg ctttcgccat cagcagcgaa ccaatcgggt tacccacgga
    28201 gcgcagcaga cccacaatac acagcaattg cagcaccgga ataatgctgt tccacttttc
    28261 gccgaatacc agcggtacga agttattcga caccaccatc agccccagca gcgccggaaa
    28321 gttgataatc cccactatcg acagcagctt gtagaagtta acacgcagct tttcggtgtc
    28381 gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg atgattgggt tcagcttcat
    28441 cggcggtacg acggccacgt tgtacgccag gttgtatccc cctgccacgc ccgcgccaag
    28501 aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga tagttgatga tgctgtccgc
    28561 cgtcagccag gcaccaaaac gtaagttcgg tgccacagac gccagcgaga agtgcagacc
    28621 ggggcaataa attttgcgtc caaagtagcc aaacagcagt gttctcaccg cactattgac
    28681 cagataaccg aggatcgcgg tcatcgccag cggccagaaa tgggcgctaa ctaccgtaaa
    28741 agtgaagccc gccagcaccg cgctggtttc gatcatgccg attttgttga actccagctc
    28801 cttctgcatc aacgcgcgga actgttgccc gtgggggatc accacaaacg ccagcgacaa
    28861 tgttttaatc aacggtgcca agtccgggtt attcagcacg tcgccgatga catcactcaa
    28921 caaaaacacc gccacacata ccacaagccc cagcccgacg ttcagccagt acagcgtggt
    28981 gagttcaagg tgactgattt cttttcgctg aataatcgag ttagcgatac cgaagtcaga
    29041 aagcgtatct gccagcgcga taatcaccag cgacacagtc agcaggccga actggtggtt
    29101 gtcgataatc cgcgccagca cggtcatctg caccagcccg aggccgatga tgatcaccgt
    29161 ggcaatagcc gaccacttcg cgccgctgat ggttttttca cgtaagctca tatcaatatg
    29221 ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac gattttgata tcgaaccaga
    29281 cgctccattc gcggatgtac tcaaggtcga actcgacgcg tttttccatt ttctccagcg
    29341 tgtcggtttc gccgcgccag ccattaatct gcgcccagcc ggtaatgccc ggtttcactt
    29401 tatggcgcag catgtagcct tcaatgagct ggcgatactg ttcgttatgc gctactgcgt
    29461 gcggacgtgg accgacaatc gacatccccc cggtcagcac attgataaac tgcggcaatt
    29521 cgtccagcga ggtgcggcgc agaaagttcc ccactttagt gacgcgcgga tcgttctgcg
    29581 tcgcctgggt caccactttg tcgttctcca tcactttcat ggaacggaac ttccacactt
    29641 tgatcggctt gccatccata ccatagcgag tctggcggaa aataaccggc cctggtgaac
    29701 tgagtttcac cgccagcgca atacagcaca gcaccgggga gatcagcagc agaataagcg
    29761 tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt aacccccgaa agcggcgtgt
    29821 catacagcgg caccaccgga acgccgttca tctcttcgag acgtgaatgg agaatgttga
    29881 aggtaaagac gtcggggatc agcagcaccg aacaggtggt atccgccagt tgatggacca
    29941 gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat atagacgtta tgaatcttgc
    30001 ccgcttttgc atcctcgacc agctgttgca ggttacccgc ccagtcgtta gaaacgccgc
    30061 ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc taaccacggc tgattacgga
    30121 aactctccat cagcatttgt ccggcggcta aatcccccac tacggcgacc atccgcttgt
    30181 tgtagccatg attacgcagc cagcccgccc caatgcgaat acatgaacgg caaaccacca
    30241 gtccgatgct ggtcagccca taccacgcca gccagatttt cagttgcgtg tcgaaatcat
    30301 tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa ggtccagttt tgtagcaaca
    30361 gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg ataaaaatcg gtgatgccgc
    30421 ccagcatctg gaacaccacc agcgtaatca gcgccaccaa caggtgcatg tagaggaatg
    30481 acagtccgct gacttcgcaa accagccata gcccggcaaa catgatggtg atatctgaaa
    30541 agcgttgcac catagagatt aacgatgcat tggttttcgc tcgctcgcgc ttttttagat
    30601 ttgtcatcgt tgttcctgtt attagcccct tacccgcagt ggggtaaggg aagatccgac
    30661 attactcgtt cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc ggcacatcac
    30721 cgcgcgattc cacattcagg cgcaccaccg gttcggtatt ggaggtgcgc aggttaaagc
    30781 accagtcggc aaaggtcatg ctgatgccat cgctgcgatc caccgccagc gcctcacggc
    30841 taaaatgctg ttccacgcgg ttaatcgcct caacgggttg cgccagtttg ctgttgatct
    30901 caccgcttgc cggaaacgcc gccatccggt cgcgcaccag ttcgcccagc gtttttcctt
    30961 tcaggcacac cagttcggcg accagcagcc acgggatcat gccgctgtcg cagtaagcga
    31021 aatcacggaa gtaatggtgg gcgctcattt cgccaccgta gatggcgtct tccttgcgca
    31081 tccgttcttt aataaaggcg tgtccggttt tcgacattac cggcgtgccg cctgcagcag
    31141 tcaccacatc aatagtgttc caagagagac gcggatcatg gatgatcttc gcgccgggat
    31201 ttttttcgag gaacgcttct gccagcaggc cgacaatgta gtagccctcg ataaactgcc
    31261 ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc aaaggcaatg cccatatccg
    31321 cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca ttccggcagc agcgggttag
    31381 gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac tttgattaat tccacgggcg
    31441 cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac cggacccgct gcgccattcc
    31501 cggagttgat caccagcttg agcggcgtga gatttttgac attgatataa ccgaacaggt
    31561 gatcaacgta agcgtcacgc aggttgattt gctgatagcg accgcgtttg gtttcatcga
    31621 cgggaggaaa gtcgttggct tcagccagac gctggacgtc gcgcagtccg gtatcaccgc
    31681 tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt gtaatccatc ggattatggc
    31741 tggcagtaac ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa tagatctctt
    31801 cggtgccgga cataccgata tccagcacat cgacgcccgc atcctgtaaa ccttttgcca
    31861 gcgccagttt taaggtttcg ctggtgagac ggacatcacc gcctaacaca atggttttcg
    31921 gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca ttcagttctt
    31981 cgcctaattt cccgcgaata tcataggctt taaagcaggt taattttttc atgtcgttac
    32041 cctttttcag gcaatagttg gccctgaccg aaacggccaa ttttattttt gtcgttattt
    32101 acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc gcgtcttatc aggcctacaa
    32161 aaccgaaccg taggtcggat aaggcgttca cgccgcatcc gacaaacagc gcctgatgcg
    32221 acgtttacac ccgtccgtag cgatccgcga agcgcaccac atcatcctct tcgagataag
    32281 agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcagtgcg
    32341 tcgcccccag cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa
    32401 tggtgacttt tgccgttccc gcgaccacta cccagtgttc cgcgcggtga tggtgcatct
    32461 gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg
    32521 cgtcgataga gtcatatttg ccccacggac gatacacttc gcgatgcacc cgatgctcat
    32581 ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgtacc gcgttacggt
    32641 cggcaatcag caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg
    32701 tgaccaggcc tgattcggca tacacatagc tgttttcagt tttgtgatta atcacatcgc
    32761 cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc ccataatgaa gaccaggaac
    32821 caacatcgct ccagcccgca tccatcggca ccacaacagc atctgccgta ggttccatga
    32881 ccgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa
    32941 aatcgagatc cggatcgacg gcgctcatcg ctttttcgca ggcatcgagg atatccgggc
    33001 gatatttttt cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc
    33061 agtaatattc gccgcttgct acataggcct gggcggtttc cagattcggt ttttcgacaa
    33121 actgcgccac ttcaaaggcc accgtatcct gctcacccac agacacttca ccgcgacgaa
    33181 tatagccata accggtttct ggtagatccg gcacaatgcc aaaggtcacc agcttgcccg
    33241 cttcggcata aggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca
    33301 catgatccgc cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg
    33361 ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc tgccggttcg agaataatgt
    33421 tctcggtgag tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat
    33481 tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca
    33541 gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg
    33601 gccataaacg gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca
    33661 taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat cgagcgtgcg
    33721 ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc aggagcgcct gacgaatccc
    33781 cgccaccagc gcctcgaccg attccggttc tacgcaaacc gcaatacccg gaaaggtttc
    33841 gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc
    33901 cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac
    33961 cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg tcatacgatt gcagcgggaa
    34021 aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc
    34081 gctctgcccg acaatggcaa aaatcagcgg ttcatcgcgc aggagatcgg cagcttcaat
    34141 aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt
    34201 atccggcagg ccaagctggt tacgaagggc atcaacatcg gcgtctgcaa catgctgaaa
    34261 acgggcaatt tccgaccagt tggggaagaa gatgacgtta tccgccgcca cgcctttttc
    34321 gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg ttatcgacgt tatgcagtcc
    34381 gctacgttcg aacgccgttg ccagctgtgc cactttgccg cctttgcctt ttccggcaag
    34441 gcccagcccc agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc
    34501 agagagcttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ctacgccaat
    34561 aatgcgatcc ggcttccagc gacgttgcgc catcagtgga aagaaactgc tgacggcaaa
    34621 actgccgaga tgcaacaagc gtttcagggt gctcggctgt tttggcacgt acagcgggca
    34681 gcgccacacc gtggcagccc cttcttctcg tttgtagcgc caggcggaat agttctcacc
    34741 cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc cgcacctcat gaccttgtgc
    34801 cgccaaccat tccaccatct cgccggtgta tttgccgatg ccggttaact ccggcgagta
    34861 gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga
    34921 aaataggctc ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc
    34981 cagcggtaat cgtcatgctg ctcatccggc agtaacagct cttcttccgc tactctgaag
    35041 cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa
    35101 aaatgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc
    35161 gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc
    35221 ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcgccg
    35281 cgactgttct cgacaataaa gtcgagagag acaagcggtg tggagcgcac tacagtggca
    35341 aagtcttcct gacgtaaaaa catcattccc cccgaaagcg gtcttgattc tcaaggaacc
    35401 actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat
    35461 gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa
    35521 ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc
    35581 agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ttgggtattc tccagccaga
    35641 cttcatgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga
    35701 attcacgcat cggtgtaccg ctgccccata ccaccacgtc cgtgccgtcg cctcgtggaa
    35761 gcgacgcagc aatgctggta tcacatggga gttactcggg tggaagttgt catgcggccc
    35821 gtacaggttg gtcggcatga cagtgcggta atcgcgtcca tactggcggt tgtaagattc
    35881 gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg gctccagcgt
    35941 gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt agatgcagga
    36001 cgatccgaga aacatcagtt tgttcacgtc gttctgatgc gcggcgtgaa tgatgttgct
    36061 ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg caacaatgcc
    36121 gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa agaaatcatg
    36181 cacggcgcgg ctgtccagca ggttcagttc atcgcgggta cgtaatacca gctccacatc
    36241 accgcgctgt tcgagctgcc gcttgatggc agaaccgacc atcccacaat ggccagcgat
    36301 aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc gtagccgtga
    36361 gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat ttcagacacc
    36421 atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt ggtcgggtcg
    36481 ccgagcagcg tttcaacttc tgccggacgg aagtaacgcg gatcaacggc gataatcaca
    36541 tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat gcccttctct
    36601 tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgctgccgc catttccaca
    36661 aactgacgta cggagtactg aacgccggtc gcgataacga aatcttccgg ctgttcctgt
    36721 tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc acgcagggaa
    36781 tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc gattgcgcgg
    36841 gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt gaagagaatt
    36901 ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca gtaggcgtac
    36961 agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt ctgcggaatt
    37021 tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt tttcttttcc
    37081 agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc gacgtcagcg
    37141 gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcctag gttgtacact
    37201 tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact caggtcgcca
    37261 taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg atccacgcgc
    37321 tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc tttttccagc
    37381 agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag agcgactttt
    37441 gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt cgcgtatcac
    37501 cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa cactgcttcg
    37561 tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga catcggttgc
    37621 cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat gttgacttgc
    37681 atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat gtgcgccaat
    37741 ggttatttca ccgagggtat ataaattgac gtcatcgcca acccacgcgt aatcacctaa
    37801 ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt tttttcctat
    37861 ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt gtggcgacca
    37921 ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt tgccgccccg
    37981 gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt atattcagac
    38041 tttgttatat aaggctttag tcttacctgt cgtacgttgg cgtaaatgcc aggataattc
    38101 tgcccagaag ccaggtacat gtaatatctg ccgttggact tttttggcgt cagcac
//
LOCUS       DBKELA010000028        37631 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.028, whole genome shotgun sequence.
ACCESSION   DBKELA010000028 DBKELA010000000
VERSION     DBKELA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.028"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..176)
                     /locus_tag="ACUQR0_001427"
     CDS             complement(<1..176)
                     /locus_tag="ACUQR0_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HHV9108534.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACUQR0_001428"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACUQR0_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HHV9108535.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACUQR0_001429"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACUQR0_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HHV9108536.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACUQR0_001430"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACUQR0_001430"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HHV9108537.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACUQR0_001431"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACUQR0_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HHV9108538.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACUQR0_001432"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACUQR0_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HHV9108539.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="ACUQR0_001433"
     CDS             complement(5180..5422)
                     /locus_tag="ACUQR0_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HHV9108540.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACUQR0_001434"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACUQR0_001434"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HHV9108541.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACUQR0_001435"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACUQR0_001435"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HHV9108542.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="ACUQR0_001436"
     CDS             complement(8339..9313)
                     /locus_tag="ACUQR0_001436"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HHV9108543.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="ACUQR0_001437"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="ACUQR0_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HHV9108544.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="ACUQR0_001438"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="ACUQR0_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HHV9108545.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="ACUQR0_001439"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="ACUQR0_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HHV9108546.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="ACUQR0_001440"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="ACUQR0_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HHV9108547.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="ACUQR0_001441"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="ACUQR0_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HHV9108548.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="ACUQR0_001442"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="ACUQR0_001442"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HHV9108549.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="ACUQR0_001443"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="ACUQR0_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HHV9108550.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="ACUQR0_001444"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="ACUQR0_001444"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HHV9108551.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACUQR0_001445"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACUQR0_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HHV9108552.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /gene="aaeX"
                     /locus_tag="ACUQR0_001446"
     CDS             23180..23383
                     /gene="aaeX"
                     /locus_tag="ACUQR0_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HHV9108553.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="ACUQR0_001447"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="ACUQR0_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HHV9108554.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="ACUQR0_001448"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="ACUQR0_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HHV9108555.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="ACUQR0_001449"
     CDS             26388..26660
                     /locus_tag="ACUQR0_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HHV9108556.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACUQR0_001450"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACUQR0_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HHV9108557.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACUQR0_001451"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACUQR0_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HHV9108558.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="ACUQR0_001452"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="ACUQR0_001452"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HHV9108559.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACUQR0_001453"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACUQR0_001453"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HHV9108560.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="ACUQR0_001454"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="ACUQR0_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /gene="zapG"
                     /locus_tag="ACUQR0_001455"
     CDS             complement(31907..32305)
                     /gene="zapG"
                     /locus_tag="ACUQR0_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HHV9108561.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="ACUQR0_001456"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="ACUQR0_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HHV9108562.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33846..34274
                     /gene="rplM"
                     /locus_tag="ACUQR0_001457"
     CDS             33846..34274
                     /gene="rplM"
                     /locus_tag="ACUQR0_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HHV9108563.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34290..34682
                     /gene="rpsI"
                     /locus_tag="ACUQR0_001458"
     CDS             34290..34682
                     /gene="rpsI"
                     /locus_tag="ACUQR0_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HHV9108564.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35077..35715
                     /gene="sspA"
                     /locus_tag="ACUQR0_001459"
     CDS             35077..35715
                     /gene="sspA"
                     /locus_tag="ACUQR0_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HHV9108565.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35721..36218
                     /gene="sspB"
                     /locus_tag="ACUQR0_001460"
     CDS             35721..36218
                     /gene="sspB"
                     /locus_tag="ACUQR0_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HHV9108566.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVCFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="ACUQR0_001461"
     CDS             complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="ACUQR0_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HHV9108567.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9416 c         9899 g         9201 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatacc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgtttg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggggg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gtctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 caggcaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctaggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacagcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttccccaaa
    33721 actttttgtg tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg
    33781 aacctcaaca attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc
    33841 ttttaatgaa aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg
    33901 acgcgaccgg taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta
    33961 agcacaaagc ggaatatact ccgcacgtag ataccggtga ttacatcatc gttctgaacg
    34021 ctgacaaagt tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg
    34081 gccacatcgg tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc
    34141 gtgtgattga aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt
    34201 tccgtaaact gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag
    34261 ttcttgacat ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc
    34321 gccgcaaaag ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca
    34381 accaacgttc tctggaacag tacttcggtc gtgaaactgc ccgcatggta gttcgtcagc
    34441 cgctggaact ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg
    34501 gtatctctgg tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg
    34561 acgagtccct gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg
    34621 ttgaacgtaa gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt
    34681 aattggcttc tgctacggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt
    34741 tatggctaaa ttctgaatca gcgtaaaaaa tgggaagttg ttttttgctg ccgtctgaaa
    34801 gacaggtaaa acaaaaacca catcgccaat aagggtctaa gtcaactatt tcagactaaa
    34861 gcgcatctct ttttccccat ttccggcatc cactcaccac aaaggtcgca aaatctggta
    34921 aactatcatc caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg
    34981 aacaaagaga gtcgttcctt attgggtaac acaacttctg actggccacc tggtggctgg
    35041 tagcagtaaa aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt
    35101 tcggtaatga cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg
    35161 ctggctgaga aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag
    35221 gatctgattg acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc
    35281 ctgtgggaat ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg
    35341 atgcctgttt acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa
    35401 gactggtaca cgctgatgaa cactatcatc aacggttcag cttctgaagc agatgccgca
    35461 cgtaagcaac tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac
    35521 ttcctgagcg atgagttcag cctggtcgat tgctaccttg ctccgctgct gtggcgtctg
    35581 ccgcaactgg gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc
    35641 cgcgtctttg agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt
    35701 ctgggccgga gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc
    35761 tgcgtgcatt ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg
    35821 tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca
    35881 acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg tgctttaacg
    35941 cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct
    36001 acgcccgtga aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata
    36061 ccagcatcat gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg
    36121 atggcgacaa gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac
    36181 cacgcggtgg tcgaccggca ttacgcgttg tgaagtaata caaaacaggc ccaggcggcc
    36241 tgttttgtct ttttaatgac ttaataaaat agcgtgattg tggcaatcat attcaccacg
    36301 aaaccgactg ccatggggat cgctgtccgc ttcaccaccg caaaaggcga aacgcctgca
    36361 attcctgacg taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg
    36421 gcaaaatgca tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc
    36481 ggaataagac tggcaaatga cataaacggc gcattgccag agcccatcac aatggcacaa
    36541 atggcaatga ctagcgccat aataatcatc acgccaatac cgcccagacc agaatgctcc
    36601 gcacccctga taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca
    36661 cccgcgacca ccagcgttac cacgttgaca aactgcgtac ccatgccgtc aaaaaacgcc
    36721 tgcacatcgt ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc
    36781 accgtcaaac ttagtaacat caccaccacc agatgcagtt ctgacttcat cagcccgacg
    36841 tgggcgagga acagcgagcc gagcatcagg attaacggca tcacaggtaa aatggcgtaa
    36901 tagagcggcg ggacattatc gagagttttt tgctctgcct gttcgtgatt gatattttta
    36961 tcttttttgt caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg
    37021 caagaggcga ccggaagctg gtagtggaag aagtatgtgg caattttcat tcccgcgacc
    37081 tgggctgcaa aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg
    37141 ataaccgcca ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg
    37201 accatcagca acatacccag accggaggcg ctggtaataa actgcgccat gatttggccg
    37261 atgacgtaag ttgccgacag aataatatac ggcgagcgaa tgagttttaa cgggcggctt
    37321 agcaagctca ccatcgcgca actggctcct atgcgctcca tgtagcgggc ataaccgccc
    37381 accgccataa tcgacagccc caatccggca atgcggttgg acagcatgcg caggatttca
    37441 ttataaatgt cgacgatcaa gtagccgctg cttttggcgg gatcgagtac accaccgaac
    37501 cctaaccaga ctccgcacat catcaggaag acacctgcgg cagccagcac cacctgaggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    37621 ccgaacattg t
//
LOCUS       DBKELA010000029        36821 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.029, whole genome shotgun sequence.
ACCESSION   DBKELA010000029 DBKELA010000000
VERSION     DBKELA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.029"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..245
                     /locus_tag="ACUQR0_001462"
     CDS             <1..245
                     /locus_tag="ACUQR0_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HHV9108568.1"
                     /translation="TIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIE
                     QRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(292..1050)
                     /gene="deoR"
                     /locus_tag="ACUQR0_001463"
     CDS             complement(292..1050)
                     /gene="deoR"
                     /locus_tag="ACUQR0_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HHV9108569.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="ACUQR0_001464"
     CDS             complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="ACUQR0_001464"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HHV9108570.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1989..3215
                     /gene="mdfA"
                     /locus_tag="ACUQR0_001465"
     CDS             1989..3215
                     /gene="mdfA"
                     /locus_tag="ACUQR0_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HHV9108571.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3256..3540)
                     /gene="ybjH"
                     /locus_tag="ACUQR0_001466"
     CDS             complement(3256..3540)
                     /gene="ybjH"
                     /locus_tag="ACUQR0_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HHV9108572.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3626..4441)
                     /gene="ybjI"
                     /locus_tag="ACUQR0_001467"
     CDS             complement(3626..4441)
                     /gene="ybjI"
                     /locus_tag="ACUQR0_001467"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HHV9108573.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4441..5649)
                     /locus_tag="ACUQR0_001468"
     CDS             complement(4441..5649)
                     /locus_tag="ACUQR0_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9108574.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5733..6269
                     /gene="rcdA"
                     /locus_tag="ACUQR0_001469"
     CDS             5733..6269
                     /gene="rcdA"
                     /locus_tag="ACUQR0_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HHV9108575.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6444..8129)
                     /locus_tag="ACUQR0_001470"
     CDS             complement(6444..8129)
                     /locus_tag="ACUQR0_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HHV9108576.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8399..8776
                     /locus_tag="ACUQR0_001471"
     CDS             8399..8776
                     /locus_tag="ACUQR0_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HHV9108577.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8806..9063)
                     /gene="grxA"
                     /locus_tag="ACUQR0_001472"
     CDS             complement(8806..9063)
                     /gene="grxA"
                     /locus_tag="ACUQR0_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HHV9108578.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9223..9510
                     /locus_tag="ACUQR0_001473"
     CDS             9223..9510
                     /locus_tag="ACUQR0_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HHV9108579.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9494..10216
                     /gene="nfsA"
                     /locus_tag="ACUQR0_001474"
                     /pseudo
     CDS             9494..10216
                     /gene="nfsA"
                     /locus_tag="ACUQR0_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10277..11179
                     /gene="rimK"
                     /locus_tag="ACUQR0_001475"
     CDS             10277..11179
                     /gene="rimK"
                     /locus_tag="ACUQR0_001475"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HHV9108580.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11267..11743
                     /locus_tag="ACUQR0_001476"
     CDS             11267..11743
                     /locus_tag="ACUQR0_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HHV9108581.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12095..13207
                     /gene="potF"
                     /locus_tag="ACUQR0_001477"
     CDS             12095..13207
                     /gene="potF"
                     /locus_tag="ACUQR0_001477"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HHV9108582.1"
                     /translation="MTALNKKWLSGLVAGALMAVFVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13302..14435
                     /gene="potG"
                     /locus_tag="ACUQR0_001478"
     CDS             13302..14435
                     /gene="potG"
                     /locus_tag="ACUQR0_001478"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HHV9108583.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14445..15398
                     /gene="potH"
                     /locus_tag="ACUQR0_001479"
     CDS             14445..15398
                     /gene="potH"
                     /locus_tag="ACUQR0_001479"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HHV9108584.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15395..16240
                     /gene="potI"
                     /locus_tag="ACUQR0_001480"
     CDS             15395..16240
                     /gene="potI"
                     /locus_tag="ACUQR0_001480"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HHV9108585.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16300..16788
                     /locus_tag="ACUQR0_001481"
     CDS             16300..16788
                     /locus_tag="ACUQR0_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HHV9108586.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16829..17956
                     /gene="rlmC"
                     /locus_tag="ACUQR0_001482"
     CDS             16829..17956
                     /gene="rlmC"
                     /locus_tag="ACUQR0_001482"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HHV9108587.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18131..18862)
                     /gene="artJ"
                     /locus_tag="ACUQR0_001483"
     CDS             complement(18131..18862)
                     /gene="artJ"
                     /locus_tag="ACUQR0_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HHV9108588.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19154..19822)
                     /gene="artM"
                     /locus_tag="ACUQR0_001484"
     CDS             complement(19154..19822)
                     /gene="artM"
                     /locus_tag="ACUQR0_001484"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HHV9108589.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19822..20538)
                     /gene="artQ"
                     /locus_tag="ACUQR0_001485"
     CDS             complement(19822..20538)
                     /gene="artQ"
                     /locus_tag="ACUQR0_001485"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HHV9108590.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20545..21276)
                     /gene="artI"
                     /locus_tag="ACUQR0_001486"
     CDS             complement(20545..21276)
                     /gene="artI"
                     /locus_tag="ACUQR0_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HHV9108591.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21294..22022)
                     /gene="artP"
                     /locus_tag="ACUQR0_001487"
     CDS             complement(21294..22022)
                     /gene="artP"
                     /locus_tag="ACUQR0_001487"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HHV9108592.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22240..22755)
                     /locus_tag="ACUQR0_001488"
     CDS             complement(22240..22755)
                     /locus_tag="ACUQR0_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HHV9108593.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23404..25173
                     /locus_tag="ACUQR0_001489"
     CDS             23404..25173
                     /locus_tag="ACUQR0_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HHV9108594.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25384..25707
                     /locus_tag="ACUQR0_001490"
     CDS             25384..25707
                     /locus_tag="ACUQR0_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HHV9108595.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25704..26534
                     /gene="amiD"
                     /locus_tag="ACUQR0_001491"
     CDS             25704..26534
                     /gene="amiD"
                     /locus_tag="ACUQR0_001491"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HHV9108596.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26531..27544)
                     /locus_tag="ACUQR0_001492"
     CDS             complement(26531..27544)
                     /locus_tag="ACUQR0_001492"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HHV9108597.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27643..29073)
                     /locus_tag="ACUQR0_001493"
     CDS             complement(27643..29073)
                     /locus_tag="ACUQR0_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HHV9108598.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29084..30085)
                     /gene="ltaE"
                     /locus_tag="ACUQR0_001494"
     CDS             complement(29084..30085)
                     /gene="ltaE"
                     /locus_tag="ACUQR0_001494"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HHV9108599.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30122..31840)
                     /gene="poxB"
                     /locus_tag="ACUQR0_001495"
     CDS             complement(30122..31840)
                     /gene="poxB"
                     /locus_tag="ACUQR0_001495"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HHV9108600.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31973..32941)
                     /gene="hcr"
                     /locus_tag="ACUQR0_001496"
     CDS             complement(31973..32941)
                     /gene="hcr"
                     /locus_tag="ACUQR0_001496"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HHV9108601.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32953..34605)
                     /gene="hcp"
                     /locus_tag="ACUQR0_001497"
                     /gene_synonym="priS"
     CDS             complement(32953..34605)
                     /gene="hcp"
                     /locus_tag="ACUQR0_001497"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HHV9108602.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34749..35648)
                     /gene="lysO"
                     /locus_tag="ACUQR0_001498"
     CDS             complement(34749..35648)
                     /gene="lysO"
                     /locus_tag="ACUQR0_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HHV9108603.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36105..36800)
                     /gene="aqpZ"
                     /locus_tag="ACUQR0_001499"
     CDS             complement(36105..36800)
                     /gene="aqpZ"
                     /locus_tag="ACUQR0_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HHV9108604.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9212 a         9153 c         9437 g         9019 t
ORIGIN      
        1 tgaccatacc gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc
       61 agcttaccgc accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg
      121 gtaaatccat tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct
      181 ttggtaggat gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct
      241 cttaatcttc tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat
      301 caacttaata cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact
      361 caccacaata tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt
      421 actgtggtcg acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg
      481 caactcttca agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata
      541 aaaagcgata tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat
      601 ggcgttgctg gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt
      661 cagcgccaga aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat
      721 cgcttcaata atccacggcg tggtggtgcc acagtcaaaa aagagggtct gatcgggttc
      781 taccagtgtc gcagccagtt ttgccgcccg gcgtttttct tccaccaggc gggatttttg
      841 atcgcttaac aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa
      901 aacgacgggc gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag
      961 cagggcggcg gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg
     1021 cccgatacgc tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc
     1081 gctcaatact acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa
     1141 tgcaaaacag acgcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg
     1201 ccagataatc tgggcactaa ggcagccaat catacctgcc agcaatccac cgagcatatc
     1261 cagcggccag tggacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat
     1321 taaaagtgag ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat
     1381 caccgtaccg tgatcgctcg ggaatgagtc atccgccgca tgatgcagga agttatagcc
     1441 gatattttcg acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa
     1501 caggctgacc gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc
     1561 ccacaaccaa agtaccgcgg ccagcaacgg caccacggta atcaaatctt tagcaataaa
     1621 aatcgccaac gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag
     1681 agagagattc aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat
     1741 acaggcaagt cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt
     1801 tccctcaagc ggcctgttta cggtgggtga ttgtaacggg cataggttaa ataaaactta
     1861 aagaaagcgt agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac
     1921 aaaattcgct ttctcgttag ctgcgctttt attaaactct gcgcgattat tattggcgaa
     1981 gaaattgcat gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt
     2041 tccctctctg tctggtgctt tacgaatttt caacctatat cggcaacgat atgatccaac
     2101 ccggtatgtt ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcga
     2161 tgaccgcgta tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc
     2221 gtattggtcg ccgtccggtg atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg
     2281 caatattact ggcgcaaaac attgaacaat tcaccctgtt gcgcttcttg cagggcataa
     2341 gcttctgttt cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg
     2401 tttgtatcaa gatcaccgcg ctgatggcga acgtggcgct gattgctccg ctacttggtc
     2461 cgctggtggg cgcggcgtgg atccatgtgc tgccttggga ggggatgttt gttttgtttg
     2521 ccgcattggc agcgatctcc tttttcggtc tgcaacgagc tatgcctgaa accgccatgc
     2581 gtataggcga gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga
     2641 acggccgctt tgtggcgggg gcgctgggat tcgttagcct gccattgctg gcgtggatcg
     2701 cccagtcgcc gattatcatc attaccgggg agcagttgag cagctatgaa tatggcttgc
     2761 tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct
     2821 cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt
     2881 tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt
     2941 taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga ttaaccctgt
     3001 ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga
     3061 tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt
     3121 ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa
     3181 aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga
     3241 tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc
     3301 acctacgctg gcaagatggc gaacataagg atgcgggcgg taagccggag ctggcgcagg
     3361 agccacatag acggtttgcg gcacagtcgc gacgctgacc gcttgtggaa cgctgactga
     3421 atcaggaacc acaaccactt tgtatccact cggcacatcg acggtgacac tttgcgccat
     3481 cgccacacca gtaaagccca ttaaaagcgc gcccaacagt agacaatttt tcataatcat
     3541 ttcgcctgaa ataatgcagc gggttaattt ccgcgaatta tgcagccttc attcaggcat
     3601 tgtgttgccg ggcgtgctgc cgtttttatt ggtcaaatgg cgcttcgtgt ttaagaactt
     3661 tatcgatcac atccagtacg ccttcacggt tattgtagcc tgcccggtat tttgccgctg
     3721 cgacgactgc gctgccggca ttttccattg caaaactaaa gcctgcctga cgcagcatct
     3781 caatatcgtt accgccatcg ccaaagacca ccacttcgct gtcgtctatt ccccataatt
     3841 tctgcagttg gcgaaggcca ttggctttat gtacgccggg gataatcaga tcgatgctgc
     3901 cgttgccggt gtggactggc accataatat cgccgatggc ctcatgtaat gctttttgta
     3961 cttgtggaat cagttcatcg gaaagattca ggccaaactt aaagaaaata tcttctaagt
     4021 tgtcaaagtt atcgacgtat tccagacggt gataatacat ttccgccacc gttttcatgg
     4081 catcgtcata ttttttgagt gtataggcgc tattttttcc gcaggcaata atttccactt
     4141 ccgggcgcgt cagcaaatgt tccacgacag tagcaaacgc atccttcgac agctcgccat
     4201 taaaaacatc tttgccttca ctcattaccc agccgccgtt ttcagccaca aaggcaattt
     4261 cattcgcaat ttcagggaag aaagagatca actgataata ttgattcccg ctggcgacca
     4321 caaagcgaat gccttgtgct ttcatttgct gatactgagc cataaaccgc tcacggttat
     4381 aggttttttg atcgcttaag aaagtaccat ccatgtctac cgcaattaat ttaatgctca
     4441 tcaactattc tccatcgccg tctgcgtttt ggtatcgggt ttggcgacgg ctttcgcgac
     4501 aatagccgcg agaataacca gcgccagtac aaccagcatt gcactacgta atccataatg
     4561 ttcgccgaga tagcccagca gcggcggccc gacgaggaaa gccagataac cggtcgttgc
     4621 taccacactg acgcgggttg gtgcatcggg gccggtatcg ctggcggcag aaatggtcag
     4681 cgggaagccc agcgaggcac ccagtcccca aagtacaaca gacactcctg cgacccaggc
     4741 gctatcgaca aaaataatca gcccaatacc caacgccccc attagtgcac tggcccgaac
     4801 cacggcaacg cgactgtaac tgtcgatgaa ccaaccgccg gtgaagcgtc caacggtcat
     4861 ccccagggta aaaccggcat aaatcagtga gccggaagtg gggctaaaac cgtgaccatc
     4921 aaccattaat aagggtaacc agtcgttggc agaaccttcg gcaaaggcca tcgccagcac
     4981 tacaacacca atcaacagca actggatatc gcgataaaaa ggaatgcctt tttcgccatg
     5041 ctgggtgcca tcggcagcat ttttgcccgt accgtcaggg attgcctgaa tggcgatata
     5101 aataggcgcg atacctacca gcgccgccaa taaaatgtgc accgttgccg gaacgccaaa
     5161 ggccgtcagt gccatcccga caccagcgcc tgccagcgtg cccaggctat aaaaaccgtg
     5221 catcatcggc aaaaccgttt tattcatttc tcgctcaacg gcggcacctt caacgtttat
     5281 cgccacttcc gcagaaccaa aacttgcccc aaagacgccg agaccaacgg caaagagcag
     5341 gggcgatgtc agccagagtg ccagacttaa tatcatcatc ccgatcaatg cgcaggacat
     5401 cgtgaccagg atgacattac gtgtcccaaa gcgtttcact aaccacgccg agcagagaat
     5461 accgctcatc gaaccgatcg acagaccaaa gagaacaccg cccatttcag cgatcgagac
     5521 agagagaata tcgcggatag caggcgtacg ggttgcccag gacgccatta acaggcctgg
     5581 caaaaagaag aacataaaca gcgcccaggt tcggcgtttc aatgcattac gtgaagaatt
     5641 tacggtcata gatcacgtca aaataagaag agggaagaca acattagcaa ggttgtgtac
     5701 atttgtacac aattgcagac agaggaaatg acatgcgtcg cgctaacgat ccgcaacggc
     5761 gagaaaaaat tatccaggcc acgctggagg cggtgaaact ttacggaata catgctgtta
     5821 cgcaccgcaa aattgctacc cttgccgggg taccgttggg atcgatgacc tactattttt
     5881 caggaattga tgagttgttg ctggaggcgt tcagcagttt tactgagatt atgtcccggc
     5941 aatatcaggc attttttagc gatgttagtg atgctcaagg cgcatgccag gctatcaccg
     6001 atatgatcta cagctcacag gttgcaacgc cggataatat ggagctgatg taccagctct
     6061 acgcgctggc tagccgaaaa ccgctattaa aaacggtaat gcaaaactgg atgcagcgca
     6121 gtcagcaaac gctcgaacaa tggtttgaac ccggaaccgc ccgcgcgctt gatgcgttta
     6181 ttgaggggat gacgctgcat tttgtcaccg accgtaagcc gctatcgcgc gaggagattt
     6241 tgaggatggt tgagagggtt gcagggtagt agataaattt tagataacaa aaaacccatc
     6301 aaccttgaac cgaaatggcg gggttgatgg gctccacaaa atggggacat caaagaaaag
     6361 cagtggcact aattaagact gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt
     6421 tcattttcag ggcaacttca gttttatcct aatcctggcc ataccatgac gatgattgtc
     6481 cctgccagcg tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc cagcgcaggg
     6541 atgttactgc gagctgtatc actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc
     6601 atcattgcgc cgaacaacag tgcgcggttc attcgcaata cataagcacc gaacaagaaa
     6661 cagataacca cgggcacaag actgacaatt aatccggcaa tcaacatctg accgccaatc
     6721 gcgcccaggc cgttattaat accgctaccg gcgctcagac caacgcctgc cataaacacc
     6781 atcaagccga actctttcac catgcttaat gcaccttgcg gaatgtaacc gaaggtcggg
     6841 tggttagcac gcataaagcc cagcataatt ccggcgaata acaacccggc agcgttcccc
     6901 atgccgaaac tgaatgtgct gaactggaag gtgatcatcc cgatcatcag cccaataaca
     6961 aagaaggcgc aaaatgccag caggtcagtg acctggctgt gaatcgagat aaagccgatg
     7021 cgatcggcga tggtttttac gcgacgggca tcaccgctga cttgtaaaac gtcacctttg
     7081 ttaagcacga cgttgtcatc tatcggcatc tcaatctggc tacgaatgac gcggttaagg
     7141 aagcaaccgt gatcggtcaa cttcagttgt gcgagacgtt tacctacagc gttatggttt
     7201 ttaacgacca cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct
     7261 ttaccgttac ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc taccaacgct
     7321 atttcatcgc ccatttgtag cacggcatca ccgtctggat ttgccagaat cccgttacgt
     7381 cgaatacgtt caatgtagca gccggtttgt cgataaatac ccagttcacg cagatttttg
     7441 ccgtcggtcc aggccaccag ctccgggccg acgcgatagg cgcggatcac cggtaaataa
     7501 accttacggt tggcatcagt gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc
     7561 tgtaagtcct gatgctgcaa tttcggcaag taacgcgcac caacaatcaa actcaccaga
     7621 ccgattaaat aggttaaggc atacccgagg ctcagattat ccagtgccag tgagagctgc
     7681 ctgctttcca tgccggaatg atgcagtgta tcgccagcac cgaccagaac cggtgtcgac
     7741 gtcatagagc ctgctaacat accggccgtc aggccaatat cccagccaaa cagcttacct
     7801 aaccctaagg cgatcaccag cgcactgcca accatcacca gtgctaacat taggtaattt
     7861 ttcccatcac gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa
     7921 ataaacagca taaagccaag attaagcgca tcggtgttaa tgctgaaatg ttgttggcct
     7981 aataacagcg atacgactaa aacgccaatg gaattaccca gttggatcga accaagtcgt
     8041 aactttccga gacatagccc aagcgcgagg accacaaata ataacagaat gtaattccca
     8101 tttaacaatt cggcgacgtt tatattcacg gaggctaact tcttgtttac tagtaagctg
     8161 ttgaaagaaa tggtaattta cgataatgtg ttttaccaga attcagggcg cagattcatt
     8221 cagcgcacct aaacgatagt aaagtaacaa tatattttac tagtgtaatc acattaggta
     8281 tcaacggcta tatgaattgc gttggcctat attagcatgg aatgcgaagc ggctttatct
     8341 tactgaacgc cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt
     8401 gaaacataaa caacgttggg cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg
     8461 cctttttctg gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg
     8521 cttgctgttt ttcattcttc ctggagcagt tgccagtttc ttttcacagc gtagagaagt
     8581 cctgaaacct ctatttggcg caatgctggc ggcaccctgt tcaatgctca ttatgcggct
     8641 gtttttttca ccgacgcgct cattctggca agagctagca tggttactaa gcgcggtgtt
     8701 ctggtgtgcg ctgggggcac tgtgtttctt atttatcagt agtttgttta aaccacagca
     8761 cagaaaaaat cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg cgtccagatt
     8821 ttctttcacc catgcagcaa aatcggtata gccgccgata tgttgctgat cgacaaaaat
     8881 ctgcggcacg gtttctacgg gtttacctgc cttttgttgt agatcttctt tagtgatccc
     8941 ttccgcacga atatctacat actgatactg aaaatcatcg cgttcattgc tcaatttctc
     9001 agccagatct tttgcacgca cacagtaagg gcaacccgaa cgaccaaaaa taacggtttg
     9061 cattatttct ctcctcatag atttatgcct gtaatgatca cgctaaaatg tattcgctga
     9121 aagtaggttt aacctgttgc attaattgct aaaagctata actgttaaac acaatacagt
     9181 gaaaagtttt agactgaagg ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa
     9241 ttgcctaaag gcgtgttgat actggaattt atcggaatga tgctactggc ggtggcgctg
     9301 ctgtcggtaa gcgactccct gtcgctgcct gagccatttt ctcggccaga agtgcagatt
     9361 ctgatgattt ttctcggtgt tttgctcatg cttcccgctg cggtggtggt tattcttcag
     9421 gtggcaaaac gtcttgcccc acagctgatg aaccgtccac cgcaatattc acgttcagaa
     9481 agagaaaaag ataatgacgc caaccattga acttatttgt ggccatcgct ccattcgcca
     9541 tttcactgat gaacccattt ccgaagcgca gtgtgaggcg attattaaca gcgcccgtgc
     9601 gacgtccagt tccagttttt tgcagtgtag tagcattatt cgcattaccg acaaagcatt
     9661 acgtgaagaa ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt
     9721 ctgggtgttc tgtgccgact ttaaccgcca tttacagatc tgtccggatg ctcagctcgg
     9781 cctggcggaa caactgttgc tcggtgtcgt tgatacggca atgatggcgc agaatgcatt
     9841 aaccgcagcg gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc gcaataatat
     9901 tgaagcggtg acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg
     9961 ccttggctgg cctgcggata atccggatct caagccgcgt ttaccggcct ccattttggt
    10021 gcatgaaaac agctatcaac cgctggataa agacgcactg gcgcagtatg acgagcaact
    10081 ggcggaatat tacctcaccc gtggcagcaa taatcgctga gatacctgga gcgatcatat
    10141 ccgccgaaca atcattaaag aaagccgccc atttattctg gattatttgc acaaacaggg
    10201 ttgggcgacg cgctaaaacc gctacgtcga tgtatgatac gcgggctttt gaccaggtct
    10261 gacagagagg tgcagggtga aaattgccat attgtcccgg gatggaacgc tctattcgtg
    10321 taagcggctg cgtgaagccg ctatacagcg cggtcacctg gttgaaattc ttgatccgct
    10381 ttcttgctac atgaacataa atcctgcggc gtcttctatt cactacaaag gccgcaagtt
    10441 accccatttt gacgcagtga tcccgcgtat tggcaccgcc attacctttt atgggacggc
    10501 ggcactgcgc cagttcgaga tgctggggag ctatccgctc aatgagtcgg tcgccattgc
    10561 ccgggcgcgt gacaaattgc gttccatgca actgctggcg cgtcagggca tcgacctgcc
    10621 tgtcacgggc attgcgcatt cgccggatga taccagcgat ttaatcgaca tggtcggtgg
    10681 tgcgccgctg gtggtcaagt tggttgaagg cacgcaggga attggcgtcg tgctggcgga
    10741 gactcgtcag gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg cgcatattct
    10801 ggtgcaggaa tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg ttgttggtga
    10861 tgaagtggtc gctgcgattg aacggcgggc gaaagagggc tattttcgtt ccaatttgca
    10921 tcgtggcggc gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa
    10981 agccgcgcga acgatggcgc tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg
    11041 gccgttggtg atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa aaaccaccgg
    11101 tatcgatatc gcgggtaaaa tgatccgctg gatcgaacgc cacgctacga cagaatattg
    11161 cctgaaaacg ggtggttagt cgcaatcaca ttactgatca tggttttgcc tgcgcttttt
    11221 gcgtaagctg tgccggtctt tttatcgaaa gaggttgtac aaaattatga catcgctggt
    11281 cgttcctggt ctggatacgc tgcgtcaatg gctcgatgac ctggggatga gtttttttga
    11341 atgtgataac tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg gtgtctttga
    11401 tgccaaaatc gatctgatcg ataacacgat cctgttttct gccatggcgg aagtccgacc
    11461 ttcagccgta ttgccgctgg cggcggattt atctgccatc aatgccagtt cgctgaccgt
    11521 gaaagcattt cttgatatgc aggatgataa tctgccaaag ctggtggttt gccagtcttt
    11581 atccgttatg cagggcgtaa cctatgagca gtttgcatgg ttcgtgcgtc agagcgaaga
    11641 gcagatttcg atggtcattc ttgaagctaa tgcccatcaa ctgctgttac cgactgacga
    11701 tgaagggcaa aacaacgtta ccgaaaacta ttttctccac tgataattcc tttcgagcac
    11761 gcagtcgctg ctgcagtgac tgcgcgctgc aaaattatct gctgttttta accttttctt
    11821 aaagattatt tcacttctct tgtgtcgatt tggctttatc acatagagca aatacgcata
    11881 aaaatttgtt aaataccgtt ttttaatccg agctatagtc tcaaaccctg gctaaagtta
    11941 ttcttgcgat gcttctatat agcgagcagt gctggccggg agagagttct cttttcttac
    12001 accgcgccga taaaaaaata tgcacgttta ttgcatatct ttcagtgtga caacttttgt
    12061 tcgtttgtta acgaactttc agaaggaaag agatgtgacc gccttaaata aaaaatggct
    12121 atcgggtctg gttgcgggtg ctctgatggc cgtctttgtc ggcacgctcg cggctgaaca
    12181 aaaaacactc cacatttata actggtctga ttatatcgcc cctgacacgg tggccaattt
    12241 tgaaaaagaa accggtatta aagtcgtcta cgatgttttc gactctaacg aagtactgga
    12301 aggcaaatta atggcgggta gcaccggctt tgacttggtg gttccatctg ccagctttct
    12361 agaacgccag ttgactgcgg gagttttcca gccgctggac aaaagcaaat tgccggagtg
    12421 gaagaatctc gacccggagc tgctgaagct ggtcgccaaa cacgatcccg acaataaatt
    12481 tgctatgccc tatatgtggg cgacgaccgg gattggctat aacgttgata aagttaaagc
    12541 tgtgctgggc gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct
    12601 ggaaaaactg aaaagctgcg gtgtctcttt cctggatgcg ccagaagaag tttttgctac
    12661 cgtgttgaat tatctcggca aagatcccaa cagcactaaa gcggatgatt acaccggacc
    12721 ggcaaccgat ctgctgttaa agctgcgccc gaacattcgt tatttccatt catctcaata
    12781 cattaacgac ctggcaaacg gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg
    12841 gcaggcgtca aaccgcgcga aggaagcgaa gaatggagtg aatgtctcgt tctcgattcc
    12901 aaaagaaggg gcgatggcgt tctttgatgt attcgccatg cctgcggatg ccaaaaacaa
    12961 agacgaagcc tatcagttcc tgaattacct gctgcgcccg gatgtagtag cgcatatttc
    13021 cgaccatgtg ttctatgtca acgccaataa agcagccacg ccgctggtga gtgcggaagt
    13081 ccgtgagaac ccgggtattt atccgcctgc ggatgttcgt gcgaagctgt tcactctgaa
    13141 agtgcaggac ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg
    13201 aaaataatcc gcagccttag atgccgggcg ggcgcaccac acccgccggc aattcgcacc
    13261 atcatggtgc gcttgcacac attcaatgcc ggagagcagc cgtgaatgac gctatccctc
    13321 gcccgcaggc gaaaacccgt aaggcgctga cgccgctatt agaaatccgc aacctcacca
    13381 aatcctacga tggtcaacat gcggtggatg atgtcagcct gaccatctac aaaggtgaaa
    13441 tcttcgcgct gctgggcgca tccggctgtg gcaagtccac gctgctgcgt atgctggcag
    13501 gtttcgaaca accttctgcc ggacagataa tgcttgatgg cgtcgatttg tcacaggttc
    13561 cgccttacct gcgccccatc aatatgatgt ttcagtctta cgcgctgttt ccgcatatga
    13621 ccgtggaaca gaacatcgct tttggcctga aacaggacaa actaccgaaa gcggaaattt
    13681 ccagtcgggt caatgagatg ctcggcctgg tgcacatgca ggagttcgcc aaacgtaaac
    13741 cgcatcagct ttctggtggt cagcgacagc gtgtggccct ggcccgaagc cttgccaagc
    13801 gcccgaaact attactgctc gacgagccga tgggcgcgct ggataaaaag ctgcgtgacc
    13861 ggatgcagct tgaagtggtg gatattctgg agcgcgtcgg tgtgacttgt gtgatggtca
    13921 cccacgatca ggaagaggcg atgaccatgg cggggcgcat cgccattatg aatcgtggga
    13981 aatttgtcca gattggcgaa ccggaagaga tctacgagca tccgactacc cgctatagcg
    14041 ccgaatttat tggctcggta aacgtctttg aaggcgtact caaagagcgt caggaagatg
    14101 gcctggtgct tgattcgccg gggctggtgc atccactgaa agtcgacgcg gatgcctcgg
    14161 tggtcgataa cgtgccggta catgtggcgc tgcgcccgga aaaaatcatg ctttgcgaag
    14221 agccgcccgc caatggttgt aacttcgcgg tgggggaggt gatacacatt gcctatctcg
    14281 gcgatctttc ggtgtatcac gttcgtctga aaagtgggca gatgattagt gcccagctac
    14341 aaaacgccca tcgtcatcgt aaagggttac cgacctgggg cgacgaagtg cgtttgtgct
    14401 gggaagtgga cagctgtgtg gtgctgacgg tttaaggagc aaagatgaat acacttgaac
    14461 ctgctgccca gtcgaaaccg ccgggcggat ttaagctgtg gttgtcgcag ctgcaaatga
    14521 agcatgggcg caaactggtc attgcgttgc catatatctg gttgatcttg ctgtttctgc
    14581 tgccatttct gattgtcttt aaaataagcc tggcggagat ggcgcgcgct attccacctt
    14641 ataccgaact gatggagtgg gctgacgggc aactgtccat cactcttaat ctcggtaatt
    14701 ttctgcaact gaccgacgat tcgctctatt tcgatgctta tctccagtcg ctacaggtgg
    14761 ctgcgatttc gacattttgc tgtttactga tcggctatcc gctggcgtgg gcggtggcgc
    14821 acagtaagcc ttcgacccgt aatattttat tactactggt gatcctgccg tcgtggacct
    14881 cgtttctgat ccgcgtttat gcttggatgg gaatattaaa aaacaacggt gtgctgaata
    14941 attttctgct gtggctgggg gttatcgatc aaccgctgac cattcttcat accaatctgg
    15001 cagtttatat tggcattgtt tacgcctacg tgccgtttat ggtgctgccg atttataccg
    15061 cgttgattcg tattgattat tcgctggtgg aagcagcgct ggatctcggt gcacgaccgc
    15121 tgaaaacgtt ctttactgtg atcgtgccgc tgactaaagg tgggattatt gccggatcga
    15181 tgctggtgtt tatcccggct gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg
    15241 acagcatcat gatcgggcgc gtgctatggc aagagttttt caataaccgc gactggccgg
    15301 tggcctcggc ggtagcgatc atcatgttgc tgctgctaat tgtgccgata atgtggttcc
    15361 acaaacacca gcaaaaaagc gtgggagaac acggatgaat aatttaccgg tagttcgttc
    15421 gccctggcgg attgtgattt tgctgctggg ctttaccttt ctctacgcgc caatgctgat
    15481 gctggttatc tattcgttta acagctcgaa gctggtgacg gtgtgggccg gctggtcaac
    15541 gcgctggtat ggtgagttat tgcgcgatga cgcgatgatg agtgcggttg gttcaagcct
    15601 gacaattgct gcctgtgcgg caacggcggc ggcgatcctc gggactattg cggcggtggt
    15661 gctggtgcgc tttggcaggt ttcgcggatc aaatggcttt gcctttatga tcaccgcgcc
    15721 gctggtaatg ccagatgtca tcacgggctt gtcgctgttg ttattattcg tcgcgcttgc
    15781 tcatgccatt ggctggcctg cggaccgcgg tatgcttacc atctggctgg cgcatgtcac
    15841 tttctgtacg gcttatgtgg cggtcgttat ttcgtcgcgt ctgcgggaac tggatcgctc
    15901 gatagaagaa gccgcgatgg atctcggtgc gacgccgctg aaagtgtttt tcgtcattac
    15961 gctaccgatg atcatgcccg cgatcatttc tggctggtta ctggctttta ctttgtcgct
    16021 tgatgatctg gtgatcgcca gctttgtttc cgggccggga gccaccacgt taccgatgct
    16081 ggtcttttcc agcgtgcgga tgggggtgaa tccggaaatc aacgccctgg caacattgat
    16141 cctcggcgcg gtcggaattg tcggatttat cgcctggtat ctgatggcgc gcgcagaaaa
    16201 acagcggata cgcgacatcc aacgtgcaag acgtggctga agacactaaa atttgccaac
    16261 ctggctacat aatgccgcgc atgtcgcggc attgttttca tggaagacga aacgttggga
    16321 ttttttaaga aaacatcttc atctcatgct cgcctgaatg tgcctgcgct ggtgcaggtg
    16381 gcggcgctcg ccattattat gatccgtggc ctcgacgtgc tgatgatttt caatacgctg
    16441 ggcgtgcgcg gtattggcga gttcattcat cgcagcgtac aaacctggag tttaacgctg
    16501 gtctttttaa gcagtctggt gctggttttc attgagatct ggtgtgcgtt ttcactggtg
    16561 aaagggcgtc gctgggcgcg ctggctatat ctgctgacac aaatcaccgc cgcaagttac
    16621 ttgtgggcgg cttcgctggg gtacggttat ccggagctgt tcagcattcc cggtgaatca
    16681 aaacgtgaaa tcttccatag cctgatgctg cagaagctgc cggatatgct catcctgatg
    16741 ctgctgttcg ttccctcgac cagtcggcgg ttcttccagt tgcaataatg tgtataatcg
    16801 tcgcccctga tgatgtgaag gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg
    16861 tcgttcctgt cagtggataa cgcagccgat tccagagcaa ctctccgcta aaaccgccga
    16921 tcttaaaaat ctgctcgccg attttccggt tgaggaatgg tgcgcgccgg tgtcaggccc
    16981 ggaacaaggg tttcgtaata aagccaaaat ggtggtgagt ggtagcgttg aaaaaacact
    17041 gctcggtatg ctgcatcgcg atggcacgcc ggaagacctt tgtgactgcc cgctttatcc
    17101 agcctcattt gcgcccgttt ttgcggcgct aaaaccgttt atcgcccgag cggggttaac
    17161 gccctacaac gtggcgcgta agcgtggcga actgaaatac attctgctga ctgaaagcca
    17221 gagtgatggc ggcatgatgc tgcgctttgt actgcgttct gataccaagc tggcgcaact
    17281 gcgtaaggcg ctgccgtggt tacacgaaca actaccgcag ctgaaagtta ttaccgtcaa
    17341 tattcagccg gtacatatgg cgattatgga aggggagacg gagatctacc tgaccgaaca
    17401 acaggcactg gcggagcgtt ttaatgacgt accgctgtgg atccgtccgc aaagtttctt
    17461 ccagactaat ccggcggtcg ccagccagtt gtacgccacc gcgcgcgact gggtacgaca
    17521 gctgccggtt aaacatatgt gggatctgtt ctgcggtgtg gggggctttg gtttacactg
    17581 cgcgacgcct gacatgcagt taaccgggat cgaaattgcg ccagaggcca ttgcctgtgc
    17641 aaagcagtca gccgctgaac tgggcttaac tcgtttacaa tttcaggcgc tggactccac
    17701 tcagtttgcc actgctcagg gggaggtgcc ggagctggtg ctggttaacc cgccgcgccg
    17761 cggcattggt aaaccgctgt gtgattatct ttcaacgatg gcaccgcgtt ttattatcta
    17821 ctccagctgt aacgcccaaa ccatggcgaa agatatccgc gaactgccag gttaccgcat
    17881 cgaacgggta cagctttttg atatgttccc gcacaccgcg cactatgaag tgttgacgct
    17941 gctggtgaag caataaaaaa gccacaggtg cggcttcaga ttgctgatca ggtgcgcttt
    18001 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaatcatgc taaatcaata
    18061 cattgcagag ttgatgtggg ccggataagc gcagcgcatc aggcaatctt acttttgtca
    18121 tcagtctcaa ttactgcgga aaccactggt cattgatttt ctgatatgta ccgtcagctt
    18181 taattgctgc cagcgcgtta ttcagttttt ccagcagagc tttgttatcc ggacgtacag
    18241 cgatacccag gccggtgccg aagtattgcg gatcggtcac tttttcagtc gcaacgccca
    18301 gttgcggatt ggttttcagc cattcgttta ccaccgcggt gtcaccaaat accccatcaa
    18361 tacgaccatt tttcagatcg ataaaggcat tctgataact gtcataggag acagttttca
    18421 cttccgggtg ctgatcctga atatatttct ggtgcgtcgt accgttttcc atcccaatgc
    18481 gtttgccttt caggtcggca aacgttttgt aggtatcttt tttggaaatc acgacggctg
    18541 agttttcata gtagggtgtg gtaaacgaaa cctgcttgct acgctccggg gtgatatcca
    18601 taccggagat tacggcgtca tattttctga atttcagtga cgggatcagg ctgtcgaacg
    18661 cgtgattagt aaaagtacat tctgcctgca tttgtttgca caaggctttt gccagatcga
    18721 tatcaaagcc gacaatctca ttattagcac ctatagattc aaagggtgga taggtggctg
    18781 aaacgccaaa attgattttc tctgcggcag aagcaccgaa agtaaaggaa gcaagtaaag
    18841 cggcaagaac taactttttc atgatggaac tcccgtctgt caatcttatg atttttggcc
    18901 gtgtctgcgg catgggataa caatgccatc aagtgaattt atatgcaata aacatgatca
    18961 aataatttaa atgaaataaa aaaagacgga taacttagtg agttgtccgt cttcattata
    19021 agaatttatg cactatgtag gccggataag gcgtccacgc cgcatccggc acaggcaccg
    19081 tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc tacaaacccc cccggcgaat
    19141 gtacgcagct acattaattc cgccgttcga atgccagtgc tttgcgctcg atcagacgca
    19201 tcatcagcgt cagcaggccg ttgacgacca ggtaaataat ccctgccgca ccgaacacca
    19261 ttacatcgta ggtgcgtccg tacaacaact ggctgtatcc catcacttcc atcagcgtaa
    19321 tggtgtaagc cagagaggta cttttgaaca ccagcaccac ttcgttggaa taagaagaga
    19381 gcgagcgttt aaaggcatac ggcagcagga tcgccagcgt atcttttttg ctcattccca
    19441 gggcgctaca ggactgccac tgaccttctg ggatcgcacg aattgcaccg taaaacagct
    19501 gcgtggtata cgccgcactg ttcagcgata acgcaatcag cgcacataac cacggttctg
    19561 acaacaaatg ccacagtgcc ggatactcct gcaacgtcgg aaattggccc ggcccgtaat
    19621 aaatcaggaa gatctgcacc agcagcggcg taccggtaaa cagcgtgata taaccccgca
    19681 ccagccacac cagcaccggc gttttcagcg tcaggatgat ggtaaaaatc aatgccagaa
    19741 tcagtgccac aatcagcgag gcaacggtta gcgtcaggct ggtgtgcagc cctttcatca
    19801 gctcgggtaa atactcaaac attagctggg cctccgctca aaacgtgtcg cgcgcaggtc
    19861 aatgcgtttg agaatgtact gactgagcag ggtgatcacc aggtaaatcg ccgccgccac
    19921 aatgtaccag gtaaatggtt cctgggtacg agtagcaatg cttttggttt gtaacattaa
    19981 atcattcaca ctaatcaaac tgaccagcgc ggtatctttc agcagcacca gccactggtt
    20041 accgaggcca ggcagcgcat gacgccacat ctgcggcatc accagacgga aaaagatagc
    20101 cgatttcgac agccccagcg cctgaccaga ttcccactgg cccaccggta cagctttcaa
    20161 cgcgccgcgc agcgtttgcg aggcataggc ggcatacagc agtgacagag cgatgacacc
    20221 acaaaggaac ggactcacgt cgaagttctc aatgtccatc tgcaccggga tctgcacgaa
    20281 cccaagattg atagtgaagc catccgaaag cgtcagcagt aactgcgagg agccaaaata
    20341 gataaacagc actaccagaa tttctggcag gccacgcaga atggttacca gcgctgaacc
    20401 tgcccacgcg acaggacgcc atttcgctga ctcccatacc gcaaagaaca tcgccagcgc
    20461 cagcccgaca atcaatgcac aaacggcaag gccgacggtc atcccggcgg cgcttgctaa
    20521 aggaaaaaat tcattcatca ggaattactt ctggaaccat ttgttgtaga tggtttcgta
    20581 agtgccatct ttcttcactt tttccagcgc agtgttgagt ttctgctgca gttcagtgtt
    20641 gccctgacgt accgcgatgc cgaggccagt accgaaataa tctttatcgg tcactttatc
    20701 gcccactgcc gccagttttg ggttatcttt cagccactca gtgaccactg cggtgtcacc
    20761 aaatacgctg tcgatacgcc cgttttgcag atccagtttt gcgttctggt agctgtcata
    20821 cggaacggtg gtgatttccg ggtgcttatc cataatgaac ttctggtgtg tcgtcccgtt
    20881 ctgaacgccg acttttttgc ctttcagctg atcaacactg gtgtatttgc cttgctgacc
    20941 cacaaacagg gcagagttgt catagtacgg ggtagtaaac agtacctgct tttcacgctc
    21001 cggagtgata tccatgcccg ccatcactgc ttctacgcga cggaatttca ggcttgggat
    21061 caggctgtca aacgcctggt tagagaaagt acaggttgca tcaatctctt tacacagtgc
    21121 ctgtgccagg tcgacgtcaa aaccaacgat ctggttgttt gcatcaatcg attcaaacgg
    21181 aggatagggg gcttcggtag caaaacgaat ggtttcggca gctgtggcgg aaagactaaa
    21241 acctgcaatt aacgcggcaa tcagaacttt tttcattgtt gttatcccga atcttagtga
    21301 gagagatagt ttttaaatgc ttcggtttgc ggctcggtaa agcagctcgc gtcgccttgt
    21361 tctacgatat gaccattttc catatacacc actcggctgg cggttttacg cgccacttca
    21421 acttcgtggg tgacgatcac ctgggtaata ttcgtttctg ccagctcacg aatgatgctg
    21481 acgatttgtg ccgtaatttc cgggtccagt gcggcggtcg gttcatcgaa cagcagtacc
    21541 tgcggttcca tcatcaacgc acgggcaata gcaacacgct gctgctgacc accagaaaga
    21601 tgcagcgggt aacgatcgct ataaggtttg agacgcagac gttccagcag tttttctgca
    21661 cgggccagcg cctgatcttt actcaacccc agtacacggc agggcgcttc aatcaggttt
    21721 tgctgcacgg tcagatgcgg ccacaggttg tattgctgaa acaccatgcc aacgttacga
    21781 cgcaaatcgc gaatcgcttt gtcagagggt gttttggtga aatcgaaatg gttgcctgca
    21841 atgttgagcg taccggagcg cggcatctca agcagattga gtacacgcag cagcgagctt
    21901 ttacccgcgc cgctggggcc aagtaacacc agcgtttcgc cctgtgggca atccagcgtg
    21961 atatcgaaca gcgcctgatg cgcgccgtag aagcaattaa tgccgtttaa ttgaatactc
    22021 attgacactc gtatactggc agtctgatag ctattgaggt cgaagatagt acctttgaca
    22081 gaataattat gcaatattta tgctttaaaa gttaaaagca aagcgcatta ttcaataaac
    22141 atagcacaaa ataacggggg cggtggtcgg tgagcataaa tgtcggcatt cctcacgaaa
    22201 tgccggataa tttacggggt ttattggttg atcaaggcgt tagcgattct cgatggactg
    22261 acggagcgta cccgccgtgg catgaacgct accgcctaag tagcgcacat cgtcgatgac
    22321 ccaacactgg ccttcctgaa tcattaacac ttcatcctgc cagccctggt caccctgttt
    22381 gagatccacc cgcaatggaa tgttacgggc atcacgatta gggatagtcg atgcactggc
    22441 aacgtgggcg ctgtctggca aggtggttcg actggagaac ggatcgctgc ttaacaattc
    22501 acgatgacta ttatcgcggc tggcatcgct aagttgggtt gccagtttgt cactcaaata
    22561 ggggcgcagg gcggtaatgt cattgctgcg atgttgaatg cggtagtcgt agaattgttg
    22621 agccacactg tcaggccctc cctggacaca ggaaccggtg cgggggccgt tatctttata
    22681 agctggcgtg acggtggtac aggcactcag cagcagtgca catggaataa gtagagatag
    22741 tgaacggtag cgcataatca tttccttatt agcttgctgt ctgcgtcaca ctacgaccaa
    22801 acgctatcag attatttaat acattgtttt taatacaatt ctgaaacaat ctgaacacct
    22861 gattgggttt gagtccattg ctggctaccc actcaaggtt gctcgtagct gacttactta
    22921 aattacccaa acaatagaca gtgtaaacca tttgtgaggt tgagcgtgtt atatggctga
    22981 aatgatcatt taaggtaaat atgatagttc tgaaatggtg gtataaatcc tcctgtagag
    23041 gttaagagca tctgcaagaa gccagtttca catttataac gcgtgataaa tgaatgcggt
    23101 tattgtctaa tcattacaaa atttttcaca tgcgataaga gatcctgcga ttaagatgat
    23161 tgccaaaatt acgttgtatt tttgaaaatg agcgaaattt gatagtccca gctttgactc
    23221 tcgataatat tatttatgct acaattgtgg gtaaagaatt taagctaatg aatttgagtg
    23281 aagaataaat taaattgctt cagtatttcg ttaattaata taaatacaaa aggcgttgtg
    23341 cttttataat tataaagcgc gaggagttcg gtggattatc gtttttcttg ggtgattttt
    23401 aatatgataa acaatatatc tgatcaagca tcatcatttc caggcacaca actaaatcaa
    23461 agtgataatt tcctggattc actgagggaa tttttcgcga tattaaattc cagtcgtaag
    23521 ggtgagttgt ctacatggga taccatctac ttgcatctga tattagcaat aaatgctgac
    23581 agtgatctca taaaaaatga tgtgttgctc gcggaaaata ttccatcagc gaattaccaa
    23641 tttaatacat tttttagcaa tactttcgag attgatgtta aaaaatatct gggtaaatca
    23701 gaagataacg aagttgaaat aaaagcgggt aatgaaagaa tctctattgg aatacgtaat
    23761 gtatcgaatg gaaaacttga aagacaacag tttcttttcc cactggacta tgagaataaa
    23821 ttacaagaac aattagataa gtattttacg attgaatcgc acccattatt atacaggtat
    23881 accattggta gtaagattgc gaatgtgatt tttgaaaagt tatacagtcg tattgacttt
    23941 aacaaagaac aatatatatc ctttataaag gacgcattta ttcattttta tgattatagc
    24001 aggcgatatg ctattagtga gaatatcgat aaagacgctg taacaaacaa tatcgcattg
    24061 atgagcactt tttatgacag tgataatacg tcaggtgagg ttttaaataa tgattttaca
    24121 gaagaagagt catttgaaac tgcgttagat gtagagcatg caatagtgct tggatttgct
    24181 gatgataatt ttgaaacaaa acctgttcat tatcaggatt tattaacacg atttagtgcc
    24241 tttcaagata ccgtgtttaa tctgtttcct gaaatgcact cttcgcatta ccacgatatt
    24301 tgtagcgtaa gtgtggatat gacaaaggga acgcaatgca tgattcactt gatggtgaat
    24361 gaagaagttt tcatgtctct gcctgttcca gttgccacga tggtacgtga agatgcaagt
    24421 aatttagtca atcttaaaac actactcaat gatggttgtt tcattaaata tagtcatttt
    24481 aatgacgtgg cattaataaa gcaaaacata agtaatctct atttatcaca cactgttgtg
    24541 aatgaaagca tattgaaaaa atgttgtttt gaaaacggca gtttaggtga tgttaaaata
    24601 acaaatagca atgtaataaa cagtgcattc aaaaatattt cgtttagatc tgttaaaatt
    24661 aataacgtaa atactcattc tttaaaattt attaactgca attttttcaa tgttgatatg
    24721 ataagagtta atctcagcaa atgtcttttt catgaatgta gtatgcatgg tgtcaagata
    24781 aaaccctggt tgcctgtaaa atggacaaaa gaattaataa gtgattattt gtatggctgt
    24841 ttactatctc tgtactctat ctgtgcaagg gatatatata atatgaacgc cgggaataac
    24901 gtgaaggttg ctgcggatgc tttccttgaa attattttct ctcttaaaaa taaatattgt
    24961 attaaactat taagcgctca ggatcgggcg tttatatacg agtttgcaag aatgatcttc
    25021 gcttatatta atgacaaaag cattgagatc ttattacttt cttgttttgc ggcggctgac
    25081 caaaaagcaa tccagcggta tagacctcag tcacaagatg gggaagactt taggagtcat
    25141 ttacaatata agttgcctct ttccgctcat taatgttgtt ggtgcaacat aataagacag
    25201 gtaagcggta ccctataatg aaaccaatat cgggttatgc aacggataat tgtaaagact
    25261 taagtgggtg tttttgacac aatgagatga tttcctaaac tgatcaaaac cttcttccca
    25321 acatggcata tcagtccgat aatgctttag agatcgaagg cttaacaaac aaggagatcg
    25381 atcatgcaat tttctacaac cccaactctg gaaggcctga ccatcgttga atattgcggt
    25441 gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc gtgatttctt tgccggtatc
    25501 cgcgatatcg ttggcggacg ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag
    25561 atcgcctttg aggaattagg ctcccaggcg cgggcgctgg gggccgatgc cgtagttggt
    25621 attgatatcg actacgaaac ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc
    25681 ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg gctggtcgct gccgctctgt
    25741 tactggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa agagggatat cagcttgata
    25801 cccgacacca ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc cactacaccg
    25861 cagatgattt tgatagctcg ctggcgacac tgactgataa gcaggtcagc tcgcattatc
    25921 tgatccctgc ggtgccaccg cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg
    25981 aacaagaact ggcctggcat gcggggatta gcgcctggcg cggggcaacg cgccttaacg
    26041 acacctctat tggcattgag ctggaaaacc gtggctggca aaaatcatcc ggagtgaaat
    26101 attttgcccc gtttgaacag gcacagattc aggcgcttat tccgctggcg aaagatatta
    26161 ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc
    26221 gcaaagacga tccggggcca ttatttccct ggcagcaact ggcgcagcag gggattggtg
    26281 cctggccgga tgcgcagcgg gtgaactttt accttgccgg gcgcgcgccg cacacaccgg
    26341 tagataccgc gtcattgctg gagcttttgg cgcgctacgg ttatgacgta aaacctgata
    26401 tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca gatgcatttc cgcccgacgt
    26461 tatataacgg cgaagcggat gcagaaactc aggcgattgc cgaagcattg ctggagaaat
    26521 acgggcagga ttagcgcggc agttttccgt ggtcgcgtag ccaggcggca gttttctcga
    26581 tgccttcatc cagggtgatg accggctgat aacctaactc ttcctgcgca cgcgtaatat
    26641 ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg ggtcagcggc ggctcttttg
    26701 ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat atccagcatc gggtaaggga
    26761 cggaacgaat acgacagtca atattcaact cgtcgatcag cttctgcacg atgctgcgca
    26821 gtgtgcgatg ctcgccgttg gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg
    26881 cttcctggct tgccagccac attgcgtgca cggcattttc atagtaggtc atatcgacca
    26941 gcgcactgcc gccatgcggt aacagaatac tgccgtagtg gtgcatcata tgcgccagac
    27001 ggggaataaa gactttatcg tgcggtccga acagactttg tgggcgcaga atagtaaagc
    27061 gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc ttcgctggct gctttgctgc
    27121 gggcaaactc gttggcgaag cggtgagggc gaaaatcttc tttaatattg cgatggtggt
    27181 gataatcgaa gtacagggag ggggaagaga tatgaataaa gttacgcaca ccccaggcga
    27241 cagcccattc acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg
    27301 tcccccaggg tgaggtaaag ctggagcagt gccacagcgt atcaatgccc gcgagcatca
    27361 ctttagcttg tgatgaaacc agctcggtca gatccgtcgg aacaaactct gcgcccattt
    27421 tttccagcaa tttacccatt gcttcgttgc gaccgctcgc acgcacgctg atgcctttct
    27481 ggcataaaaa ctctaccgcg tttcgaccta agccgctggt ggcgccggta accagtacct
    27541 tcatatcaat ccactgttgt tgagaaaatg acgtgcgcat tcttccgtga tttcccccat
    27601 gatgcaatgg gaaacatgaa agaataacgc aggttttgtc gattaatctg tgctttgttc
    27661 tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata aacagatgcg cggggatcat
    27721 caataaccag taaaacagcc ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat
    27781 agtacgatag tcacctttat cttccagggt aaaacacagt cgtcccagcc ccggcgcttt
    27841 catgccaaat aacaacgtaa gttgcttttt cggttcaacg acaatcactt tccagctatc
    27901 caccgcatcg ccggtctgta aatattcgcg ctccgggcgg cctttcgcca gattatgacc
    27961 gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata ttgccaaaga aataacgctc
    28021 tttaccgccg atttggttca ccaccagcca taaagccgca aggctggcgg acgttttaac
    28081 ggtaaacccc gcctgtttgg cgaaataacc gtactccggt cgccagcggg caaaggcctg
    28141 agcgtcgtag ccccagtcgc tggagttgac cagtttttct tcctctttca acgtgctacg
    28201 taccgcgtca tcgaaagcga tcagccgttg tgggatgagt gcacgtagcg cggtatcatc
    28261 tgccagcaga tcgtgtttca gcccctgaat caacgccctg gcggtggtgg gcggtacgga
    28321 agtaatcaca ttgagaaacc acaccgaaat ccagcgggtg gggaggggga tggggatcaa
    28381 ccagcggcgc ttaccgctca ccgccataaa atgttcaaac tgttgctgat aactgagcac
    28441 ctctggtccg gcggcttcga agatgcggtg ttcgttggct ggatggtcta acagcgccac
    28501 cagatagtgc agcaagtttt ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg
    28561 cggcgttaac actggcaggt tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc
    28621 tgcgccaacg ataattccgg cccgaagttc ggtcactggt acattcgctt cacgaagaat
    28681 gtccgccgta gcctgacgag ctcgcagatg atccgactgc tcatgtggcg gggcctgcaa
    28741 cgaactgaga aagattaatt gcttaactgg tacttcacgt agcgcatcgc ggacgttgag
    28801 agccagctgg cgctcctgag cgataaaatc gccgccttcg cccatgctgt gcaccagaaa
    28861 atagacggta tcgatatcct gcaacagggc cggaaggtta tccggccagc tgagatcgac
    28921 tttatggcaa ctgacgtttg ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc
    28981 cgctaggatc tgatgccctt gctggctgag tgtgcgcacc agatgctgac caatgtagcc
    29041 actggcaccg agaactaaaa tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg
    29101 cgcgccagtg ggcggcgact tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca
    29161 ggcggacaat cggcgaggcg ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg
    29221 cggcagcatt ttcttccccg acgcgaacaa acagcatatt agtgtcctga cgcatcacat
    29281 ccgcgcctgc ttcacgcagt tgctccgcca tccaggcggc gttgtcgtga tcttcctgca
    29341 accgtgcgac gttatttttc agggcataca tcccggcggc agccagaatc ccggactgac
    29401 gcatcccgcc accggccatt ttccgccagc ggatggcacg tttaatgtaa tcacgattac
    29461 cgacgagtaa tgaaccgact ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat
    29521 cacaatattg cgtgatctct ttcagttcgc agccgtaagc caccacggca ttaaagatgc
    29581 gcgcaccgtc aacatgcagc gccagattgc gctcgcgggt aaattcccat gcttctttca
    29641 ggtattcacg cggcagcact ttgccgttgt gggtgttttc cagactgagt aatttggtgc
    29701 gggcgaaatg gatatcgtcg ggtttgattt tcatcgccac tttatccagc ggtagcgtgc
    29761 cgtcggcagc cgcgtcgatg ggttgcggct gaatactgcc cagcaccgct gcgccaccgg
    29821 cttcaaacag atagttatgc gcggcctgac cgacaatata ctcttcgcca cgttcgcagt
    29881 gactgagcag agcgaccagg ttggcctgag tgccggtagg cagaaaaatg gcggcttctt
    29941 taccggaaag ctctgctgca tagtcctgca gagcattaac ggtagggtcg tctccgtaaa
    30001 cgtcgtcccc aaccggggcg gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa
    30061 cggtatcact gcgtaaatca atcatggcat gtccttatta tgacgggaaa tgccaccctt
    30121 tttaccttag ccagttcgtt ttcgccagtt cgatcacttc atcgccgcgt ccgctgataa
    30181 ttgcgcgcaa catatacagg ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa
    30241 tggctaattc ttctttggcg accaccacat ccaccaacac cggaccgtcg atagagaagg
    30301 cgcgttgcag ggcttcatca acttcagagg ctttttctac acggataccc gtaatgccgc
    30361 acgcttcggc aatgcgggca aagttggtgt cgtgcagctc agtaccgtct gtcaggtaac
    30421 ctccggcctt catctccatc gccacaaagc ccagcacgct gttattaaag acgataattt
    30481 tcaccggcag tttcatctgc actactgaga ggaaatcgcc catcaacatg ctaaaaccgc
    30541 catcgccgca catggcgacc acctgacgtt ccggctcggt tgcctgcgca cccaacgcct
    30601 gcggcatggc gttagccatc gaaccgtggt taaacgaacc taacaggcga cgcttgccgt
    30661 tcattttcag ataacgtgcc gcccataccg ttggcgtgcc gacgtcacag gtaaagatgg
    30721 cgtcatcggc ggcaaaatga ctaatttgct gcgccagata ttgcgggtga atggctttct
    30781 cgctcggttt agctaaatcg tccagccctt tgcgggcatc gcggtaatct tccagcgctt
    30841 tatccagaaa cttgcgatcg gttttttctt ccaccagtgg cagcaatgcg cgcagggttg
    30901 atttgatatc gccgaccagc gccatatcta ccttgctatg cgcgccgatg ctggctggat
    30961 taatatcaat ctgaataatt ttggcatcgg tcgggtagaa cgcacggtag gggaactgag
    31021 taccgagtag cactaacgtg tcggcgttca tcatggtatg gaaacctgac gagaatccga
    31081 ttaacccggt cattccaaca tcatacggat tatcgtattc gacatgctct ttaccgcgta
    31141 gggcatgaac aataggtgct ttaattttcc cggcaaactc aactaactct ttatgcgccc
    31201 ccgcgcagcc gctgccacac atcagggcga tattgctgga ataacgcagc agttgcgcca
    31261 gtttgcgtaa ctcttcttct tccggcatca cgactggctg tggcgcatga taccagtgcg
    31321 tggttgcccc ttctggcgca ggttttaacg ccacgtcgcc cggtaataca acaacggaaa
    31381 cgccacggtt aagcaccgct ttgcgcatag caattgccag cacttgtggg atctgctccg
    31441 ggctggaaac cagctcgcaa tagtgactac attcgcggaa tagctcttgt gggtgggttt
    31501 cctggaaata gccgctgcca atttcgctgg agggaatatg agcggcaatc gccagtaccg
    31561 gaacgttatt gcggtggcaa tcgaataggc cgttgatcaa gtgcaggttg cctgggccgc
    31621 acgatccggc acagaccgcc agttctcctg aaagttgtgc ttcagcgcca gcggcaaacg
    31681 ccgccacttc ttcatggcgg gtggacatcc actcgatggt gcccatgcga ttaagactgt
    31741 cgctaagacc gttcagagag tcgcccgtga ctccccagat gcgtttcacg cctgccgatt
    31801 cgagtgtttt ggcgatataa gctgcaaccg tttgtttcat ggttctccat ctcctgaatg
    31861 tgataacggt aacaagttta gttcatctga cggaggggga agggatggga gagaaaggag
    31921 gcactaacgg ttaaatagcc cgatgaaagg aatatcatcg ggcataaggc gattatgcga
    31981 gaaccaaatc cccctgcgga tggcaggagc aggccagtac gtaaccttca gcgatttcgg
    32041 cgtcggtcag cgtcattgta ctgctcaccg tatattcacc ggaaaccact tttgtcttac
    32101 agcagccgca aacacccgca cggcaggcag cgacaaccgg aacgttattg ctttccagcg
    32161 cctccagtag cgtggtgcca accggggcgt aaaattctcg tgccggttgc agtttggtga
    32221 atttcagacc gctggtcgcc gcttctgcta ctggggtgaa gaatttctct ttaaagaaac
    32281 gcgtcacgcc gagcgctttc acttcctgct ctacccaatc catatacgga gccgggccgc
    32341 aggtcatcac ggtacgtgaa gctaagtcag gtacatctgc cagcagttcg cgagtgagac
    32401 gaccagcgat aaagccttcg gtaacgttat tttctgccac cagcgttacc ggatagttac
    32461 gccactcatc ggcgaaaata acatcctgcg gcgtacgcac gttgtagatc acccgcacat
    32521 cggcctgtgg acggttcttc gcaagccagc gacgcatcga cataatcggc gtgacgccgc
    32581 agcctgccgc cagcaacagg aatttatctt ctgctttatc gtcgcaggta aattccccca
    32641 tcgcgtccga aagccagaga taatcaccgc gttttacatc gcgcgtcagc cactgggagc
    32701 cgacaccgtc atcaatccgc cgcacggtca gggtgatata ttcactcacg cctggcgtgg
    32761 aggaaatggt gtaagcacgc agcgtttccg ctgagtttcg cacgctgacc agtgcatatt
    32821 gcccggcgcg atatgggtag tgatcgtggc aaatcaggga aatcgtccac acatccggcg
    32881 tttcttgcgt aatgtgatga acctgtatcc gccacgggca ttgattcgtt ggcatcgtca
    32941 tcgacaaact ccttacgcgt tcaacagttg cttcatgtct tcttcaacag tggtgataga
    33001 acgcaggccg aatttctcgt tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc
    33061 agtcgggccg gtgacgatat ttttcacgcc cagagaaagc agcgtcagca gaatgacaat
    33121 cgctttctgt tcaaaccagg agagcaccag cgacagcggc agatcgttca caccacagcc
    33181 cagtttctct gccagagtga cagccagaat aattgctgag taagcatcgt tacattgacc
    33241 tgcatctacc agacgcggca gaccttcgat atcgccaaac tcaagtttgt taaagcgata
    33301 tttaccacag gcgagggtca ggatcaggca gtcatccggc acgctggtgg cgaaatcggt
    33361 gaagtagtgg cgctcgccgc gtgcgccgtc acagccacca agcaggaaga tatgacgcag
    33421 tttttcacgg ctaaccagat caatcagcgt atcggctgca ccaagcagcg tctggcgacc
    33481 aaagcccacg gtgatcaggt gaggaatttc gctgtacggg aagcccgcca tttgttgcgc
    33541 ctgggcgatg accgcagaga aatcttcgcc atccagatga cgcacgccag gccagccaac
    33601 gatgctgcgg gtccagatac gatcgtcata agcgcctacg gttgggtcga tgatgcagtt
    33661 cgaggtcatc acgatggggc cagggaaacg agcgaactcc acttgctgat tctgccagcc
    33721 gctgccgtag ttaccgacca gatgcttgaa tttacgcagc tccgggtagc catgcgcagg
    33781 cagcatttcg ccgtgggtgt agacattaac gcccgtgcct tcggtctgtt ccagcaggtt
    33841 gtagagatct ttgagatcgt gaccggaaat cagaatgcat ttacccgccg tcgctttgac
    33901 gttgacctgg gttggcgtcg ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct
    33961 catcactttg aagttcatct ggccgatttc cattgaacac tcaagaagcg cgttcatatc
    34021 ggcaggccag gtccccagcc acgccatgat tttatggtac tgggcataaa tatcgttgtc
    34081 gtattgaccg agaacatgcg cgtgttccat ataggccgcc gcacctttca ggccatacag
    34141 gcacagcaga cgcaggccga gaatgttttc gccaatcgcc gctttatctt tgttaggggt
    34201 aaattctgct gcctgacgtt gcagctcgcc gagatcatcg ctcaccagtt gcaggtcagc
    34261 catcgggtta tcgacgcgcg cgttggcatc tacagccagg cattgtgctt tcagcgcctc
    34321 gcgcaaggca atcgcttcac gagcgtagcc gacaatacgc ggagaatcga agttaacgtt
    34381 ggtcagggtt gagaaaaagg cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc
    34441 gtattcacgc gcttttaccg cccaggcaga aagcccttgc agcgccgcga tgagtaaatc
    34501 ctgaaggtca gaagtttccg ccgttttacc acacatcccc tgcgcgtatg agcagccgtt
    34561 ttctgccgga gtacggatag tttgttcaca ttgcacacaa aacatgatca caccttttaa
    34621 agttatattt aatatacatg tttaagggta agacgcttaa cgcggggata aaagggattt
    34681 ttcatgcaac tttaagggag attgatttag cgcaattttg gcggcaggga actaccgcca
    34741 gagaggtatt acgcagagaa aaaggcgatg aggatcggca ctaacaggct aagaataaaa
    34801 ccgtgaacaa ttgccgccgg gaccatatcc agcccgccag tacgttgaag aacgggcagg
    34861 gtgaaatcca ttgatgtggc accgcataag cccagtgcag tagagcggct gcggcgaatc
    34921 agcccaggga tcaacataat agcaatcagt tcacgggcca gatcattaaa aaacgccgcg
    34981 ctcccgatta ccggaccaaa agattcggtc aataaaatac cggaaagaga ataccagccg
    35041 aaaccggagg ccattgccag cgcggtattg atggggagat caagaataaa ggcgttaatt
    35101 aaaccaccaa ttaatgaact gacaaccacc accacggcga caatcattcc ccggcgatta
    35161 aggacaatct gctttaaggt catgccatta ttgcgcaact gaataccaac gaggaaaagt
    35221 agcaaaatta acgtgtattc actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc
    35281 agaccaatgg caaaaccaat cactactacg ccgcacagtt ttagcgactc cagcgccatc
    35341 gcaatacgcg acgggagttt ttcttgctga tggtggttgc gccacggcag gcctcgctcc
    35401 agccacatca gggcggcaat attacacagt aaaataacgg taatactgac ggcagaataa
    35461 tgcagaatcg ccaacaggtt actggcgagg ttatcgagaa acgccagact gatacccata
    35521 aaaaagagaa taaggtaaac catccagctt aatagctgat taatagcttt taacgcagct
    35581 tgttggcgaa gcggaatgag gtaacccaca atcaggggaa ccagaatgat taacagccca
    35641 gaaaacatga aaatccagtc cttgcaaaga agaagtcgaa atgcgcgatg acacactact
    35701 gaaagcggaa gggcgagtaa agttgcaatt aaaaggaaat gttatgcatc agagggggat
    35761 ttttaatcct atctgagtgt ttagcattgt tgcgctcctt tcatgtgcaa tgccatataa
    35821 agccactggc ggagggagcg cgcagggggc agccagtcgc cgccgccccc tgcacccccg
    35881 ggctctggcg aacaaaatcg ccgctgcgcg gtaccttcag cttatcccct tcggcaaccg
    35941 ggtcgggcgc gaggtaacgt cctgtaagac gcgccctcag cccacatcca tgtgggctgc
    36001 cccggccttc ggggaacgct tcagcgattt tgacgccacc aatacccgtg cggtttgtaa
    36061 tgtcgaagat aaaacaacca gggcagataa aatgccaatg ctaattaatc acgcttttcc
    36121 agcagagtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
    36181 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
    36241 gggttaacag aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga
    36301 ccaatagcga tcggcgcaaa acctgccgga gcgaatttgt cggttgcacc gtggatcact
    36361 aacaggaagc ccgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg
    36421 cctggtgaat gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc
    36481 gttttaccac tggcaattaa atacagcagc gccgctgcaa caataccgcc gacaacctgg
    36541 gcaattacgt agccaacgac ttcttttgcc ggaaaacgtc cgccagccca taaaccaata
    36601 gtgaccgccg ggttaaaatg accaccagaa atatgaccaa cagcgaaggc catcgtcaga
    36661 acggtcaaac caaatgccag cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct
    36721 gcggccagta cagcactacc acagccacca aaaacaagcc agaaagtacc aaaacattca
    36781 gctgctaatt ttctgaacat atccacctca attaaaaatt g
//
LOCUS       DBKELA010000030        36520 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.030, whole genome shotgun sequence.
ACCESSION   DBKELA010000030 DBKELA010000000
VERSION     DBKELA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.030"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..190
                     /locus_tag="ACUQR0_001500"
     CDS             <1..190
                     /locus_tag="ACUQR0_001500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108605.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACUQR0_001501"
     CDS             complement(156..1391)
                     /locus_tag="ACUQR0_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HHV9108606.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACUQR0_001502"
     tRNA            complement(1538..1614)
                     /locus_tag="ACUQR0_001502"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACUQR0_001503"
     tRNA            complement(1657..1743)
                     /locus_tag="ACUQR0_001503"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACUQR0_001504"
     tRNA            complement(1764..1839)
                     /locus_tag="ACUQR0_001504"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACUQR0_001505"
     tRNA            complement(1898..1974)
                     /locus_tag="ACUQR0_001505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /gene="thrP"
                     /locus_tag="ACUQR0_001506"
                     /gene_synonym="yifK"
     CDS             complement(2077..3462)
                     /gene="thrP"
                     /locus_tag="ACUQR0_001506"
                     /gene_synonym="yifK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015194 - L-serine transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015195 - L-threonine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine/serine APC transporter
                     ThrP"
                     /protein_id="HHV9108607.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACUQR0_001507"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACUQR0_001507"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HHV9108608.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACUQR0_001508"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACUQR0_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HHV9108609.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACUQR0_001509"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACUQR0_001509"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HHV9108610.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACUQR0_001510"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACUQR0_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HHV9108611.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACUQR0_001511"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACUQR0_001511"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HHV9108612.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACUQR0_001512"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACUQR0_001512"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HHV9108613.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACUQR0_001513"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACUQR0_001513"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HHV9108614.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACUQR0_001514"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACUQR0_001514"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HHV9108615.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACUQR0_001515"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACUQR0_001515"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HHV9108616.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACUQR0_001516"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACUQR0_001516"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HHV9108617.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACUQR0_001517"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACUQR0_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HHV9108618.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACUQR0_001518"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACUQR0_001518"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HHV9108619.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACUQR0_001519"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACUQR0_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HHV9108620.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACUQR0_001520"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACUQR0_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HHV9108621.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACUQR0_001521"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACUQR0_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HHV9108622.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACUQR0_001522"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACUQR0_001522"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HHV9108623.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACUQR0_001523"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACUQR0_001523"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HHV9108624.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACUQR0_001524"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACUQR0_001524"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HHV9108625.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACUQR0_001525"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACUQR0_001525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HHV9108626.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACUQR0_001526"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACUQR0_001526"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HHV9108627.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACUQR0_001527"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACUQR0_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HHV9108628.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACUQR0_001528"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACUQR0_001528"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HHV9108629.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACUQR0_001529"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACUQR0_001529"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HHV9108630.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACUQR0_001530"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACUQR0_001530"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HHV9108631.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACUQR0_001531"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACUQR0_001531"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HHV9108632.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACUQR0_001532"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACUQR0_001532"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HHV9108633.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACUQR0_001533"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACUQR0_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HHV9108634.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACUQR0_001534"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACUQR0_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HHV9108635.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="ACUQR0_001535"
     CDS             33502..35022
                     /locus_tag="ACUQR0_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HHV9108636.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="ACUQR0_001536"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="ACUQR0_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HHV9108637.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="ACUQR0_001537"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="ACUQR0_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HHV9108638.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="ACUQR0_001538"
     tRNA            complement(36439..36514)
                     /locus_tag="ACUQR0_001538"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8918 a         9996 c         9200 g         8406 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg tactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcatat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgtacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acattaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DBKELA010000031        34999 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.031, whole genome shotgun sequence.
ACCESSION   DBKELA010000031 DBKELA010000000
VERSION     DBKELA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.031"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..815)
                     /locus_tag="ACUQR0_001539"
     CDS             complement(<1..815)
                     /locus_tag="ACUQR0_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9108639.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACUQR0_001540"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACUQR0_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1000..1083)
                     /locus_tag="ACUQR0_001541"
                     /pseudo
     CDS             complement(1000..1083)
                     /locus_tag="ACUQR0_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACUQR0_001542"
     CDS             complement(1398..1874)
                     /locus_tag="ACUQR0_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HHV9108640.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACUQR0_001543"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACUQR0_001543"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HHV9108641.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACUQR0_001544"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACUQR0_001544"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HHV9108642.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACUQR0_001545"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACUQR0_001545"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HHV9108643.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACUQR0_001546"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACUQR0_001546"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HHV9108644.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACUQR0_001547"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACUQR0_001547"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HHV9108645.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="ACUQR0_001548"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="ACUQR0_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HHV9108646.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACUQR0_001549"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACUQR0_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HHV9108647.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="ACUQR0_001550"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="ACUQR0_001550"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HHV9108648.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="ACUQR0_001551"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="ACUQR0_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HHV9108649.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="ACUQR0_001552"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="ACUQR0_001552"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HHV9108650.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="ACUQR0_001553"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="ACUQR0_001553"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HHV9108651.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="ACUQR0_001554"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="ACUQR0_001554"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HHV9108652.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="ACUQR0_001555"
     CDS             13849..14553
                     /locus_tag="ACUQR0_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HHV9108653.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="ACUQR0_001556"
     CDS             14758..15471
                     /locus_tag="ACUQR0_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HHV9108654.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="ACUQR0_001557"
     CDS             complement(15507..16463)
                     /locus_tag="ACUQR0_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HHV9108655.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACUQR0_001558"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACUQR0_001558"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HHV9108656.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="ACUQR0_001559"
     CDS             complement(17701..18462)
                     /locus_tag="ACUQR0_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HHV9108657.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="ACUQR0_001560"
     CDS             18595..19005
                     /locus_tag="ACUQR0_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HHV9108658.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="ACUQR0_001561"
     CDS             complement(18967..20073)
                     /locus_tag="ACUQR0_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9108659.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="ACUQR0_001562"
     CDS             complement(20084..21217)
                     /locus_tag="ACUQR0_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHV9108660.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="ACUQR0_001563"
     CDS             complement(21210..22946)
                     /locus_tag="ACUQR0_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHV9108661.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACUQR0_001564"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACUQR0_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HHV9108662.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACUQR0_001565"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACUQR0_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HHV9108663.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26057
                     /gene="rhlE"
                     /locus_tag="ACUQR0_001566"
     CDS             24837..26057
                     /gene="rhlE"
                     /locus_tag="ACUQR0_001566"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HHV9108664.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNHELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACUQR0_001567"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACUQR0_001567"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HHV9108665.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="ACUQR0_001568"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="ACUQR0_001568"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HHV9108666.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="ACUQR0_001569"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="ACUQR0_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HHV9108667.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="ACUQR0_001570"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="ACUQR0_001570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HHV9108668.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31266..31325
                     /gene="ybiE"
                     /locus_tag="ACUQR0_001571"
     CDS             31266..31325
                     /gene="ybiE"
                     /locus_tag="ACUQR0_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HHV9108669.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACUQR0_001572"
     CDS             complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACUQR0_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HHV9108670.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACUQR0_001573"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACUQR0_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HHV9108671.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACUQR0_001574"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACUQR0_001574"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HHV9108672.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34999)
                     /locus_tag="ACUQR0_001575"
                     /pseudo
     CDS             complement(33012..34999)
                     /locus_tag="ACUQR0_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8295 a         8974 c         9458 g         8272 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctagcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttgccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gttatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aacaggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgaccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaat gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg tgaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 attatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aaccatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgcgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgccgg
//
LOCUS       DBKELA010000032        34444 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.032, whole genome shotgun sequence.
ACCESSION   DBKELA010000032 DBKELA010000000
VERSION     DBKELA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.032"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..332)
                     /locus_tag="ACUQR0_001576"
     CDS             complement(<1..332)
                     /locus_tag="ACUQR0_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HHV9108673.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            494..550
                     /gene="ysgD"
                     /locus_tag="ACUQR0_001577"
     CDS             494..550
                     /gene="ysgD"
                     /locus_tag="ACUQR0_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HHV9108674.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="ACUQR0_001578"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="ACUQR0_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HHV9108675.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="ACUQR0_001579"
     CDS             complement(1693..2073)
                     /locus_tag="ACUQR0_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HHV9108676.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2467)
                     /gene="yigG"
                     /locus_tag="ACUQR0_001580"
     CDS             complement(2087..2467)
                     /gene="yigG"
                     /locus_tag="ACUQR0_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HHV9108677.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="ACUQR0_001581"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="ACUQR0_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HHV9108678.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /gene="yigI"
                     /locus_tag="ACUQR0_001582"
     CDS             complement(3504..3971)
                     /gene="yigI"
                     /locus_tag="ACUQR0_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HHV9108679.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="ACUQR0_001583"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="ACUQR0_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HHV9108680.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="ACUQR0_001584"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="ACUQR0_001584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HHV9108681.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="ACUQR0_001585"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="ACUQR0_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HHV9108682.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="ACUQR0_001586"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="ACUQR0_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HHV9108683.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="ACUQR0_001587"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="ACUQR0_001587"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HHV9108684.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="ACUQR0_001588"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="ACUQR0_001588"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HHV9108685.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="ACUQR0_001589"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="ACUQR0_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HHV9108686.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="ACUQR0_001590"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="ACUQR0_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HHV9108687.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="ACUQR0_001591"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="ACUQR0_001591"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HHV9108688.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="ACUQR0_001592"
     CDS             complement(14626..15441)
                     /locus_tag="ACUQR0_001592"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HHV9108689.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="ACUQR0_001593"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="ACUQR0_001593"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HHV9108690.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="ACUQR0_001594"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="ACUQR0_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HHV9108691.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="ACUQR0_001595"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="ACUQR0_001595"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HHV9108692.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="ACUQR0_001596"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="ACUQR0_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HHV9108693.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="ACUQR0_001597"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="ACUQR0_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HHV9108694.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="ACUQR0_001598"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="ACUQR0_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HHV9108695.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="ACUQR0_001599"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="ACUQR0_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HHV9108696.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="ACUQR0_001600"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="ACUQR0_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HHV9108697.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="ACUQR0_001601"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="ACUQR0_001601"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HHV9108698.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="ACUQR0_001602"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="ACUQR0_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HHV9108699.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="ACUQR0_001603"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="ACUQR0_001603"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HHV9108700.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="ACUQR0_001604"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="ACUQR0_001604"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HHV9108701.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="ACUQR0_001605"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="ACUQR0_001605"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HHV9108702.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="ACUQR0_001606"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="ACUQR0_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HHV9108703.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="ACUQR0_001607"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="ACUQR0_001607"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HHV9108704.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="ACUQR0_001608"
     CDS             31574..32188
                     /locus_tag="ACUQR0_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HHV9108705.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="ACUQR0_001609"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="ACUQR0_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HHV9108706.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="ACUQR0_001610"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="ACUQR0_001610"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HHV9108707.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8104 a         8702 c         9305 g         8333 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa aatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgcatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg tgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    34321 actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaattccg
    34381 acagagaaag caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgaacaccct
    34441 gcgc
//
LOCUS       DBKELA010000033        34086 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.033, whole genome shotgun sequence.
ACCESSION   DBKELA010000033 DBKELA010000000
VERSION     DBKELA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.033"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..578
                     /locus_tag="ACUQR0_001611"
     CDS             <1..578
                     /locus_tag="ACUQR0_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHV9108708.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="ACUQR0_001612"
     CDS             complement(575..1711)
                     /locus_tag="ACUQR0_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9108709.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="ACUQR0_001613"
     CDS             complement(1717..2040)
                     /locus_tag="ACUQR0_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HHV9108710.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="ACUQR0_001614"
     CDS             2223..2366
                     /locus_tag="ACUQR0_001614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108711.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="ACUQR0_001615"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="ACUQR0_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HHV9108712.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="ACUQR0_001616"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="ACUQR0_001616"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HHV9108713.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="ACUQR0_001617"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="ACUQR0_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HHV9108714.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="ACUQR0_001618"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="ACUQR0_001618"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HHV9108715.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="ACUQR0_001619"
     CDS             complement(8959..9567)
                     /locus_tag="ACUQR0_001619"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HHV9108716.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="ACUQR0_001620"
     CDS             complement(9638..10723)
                     /locus_tag="ACUQR0_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9108717.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="ACUQR0_001621"
     CDS             complement(10726..11088)
                     /locus_tag="ACUQR0_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HHV9108718.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /gene="mtlA"
                     /locus_tag="ACUQR0_001622"
     CDS             11625..13538
                     /gene="mtlA"
                     /locus_tag="ACUQR0_001622"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HHV9108719.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="ACUQR0_001623"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="ACUQR0_001623"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HHV9108720.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="ACUQR0_001624"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="ACUQR0_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HHV9108721.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /gene="yibT"
                     /locus_tag="ACUQR0_001625"
     CDS             complement(15380..15589)
                     /gene="yibT"
                     /locus_tag="ACUQR0_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HHV9108722.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="ACUQR0_001626"
     CDS             15873..16235
                     /locus_tag="ACUQR0_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HHV9108723.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16779..17462
                     /locus_tag="ACUQR0_001627"
     CDS             16779..17462
                     /locus_tag="ACUQR0_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HHV9108724.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17506..21871
                     /locus_tag="ACUQR0_001628"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="ACUQR0_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="ACUQR0_001629"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="ACUQR0_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HHV9108725.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="ACUQR0_001630"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="ACUQR0_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HHV9108726.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="ACUQR0_001631"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="ACUQR0_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HHV9108727.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="ACUQR0_001632"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="ACUQR0_001632"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HHV9108728.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="ACUQR0_001633"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="ACUQR0_001633"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HHV9108729.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="ACUQR0_001634"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="ACUQR0_001634"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HHV9108730.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="ACUQR0_001635"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="ACUQR0_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HHV9108731.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="ACUQR0_001636"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="ACUQR0_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HHV9108732.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="ACUQR0_001637"
     CDS             complement(29422..29853)
                     /locus_tag="ACUQR0_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHV9108733.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="ACUQR0_001638"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="ACUQR0_001638"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HHV9108734.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31676..32935
                     /gene="envC"
                     /locus_tag="ACUQR0_001639"
     CDS             31676..32935
                     /gene="envC"
                     /locus_tag="ACUQR0_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HHV9108735.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="ACUQR0_001640"
     CDS             32939..33898
                     /locus_tag="ACUQR0_001640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HHV9108736.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34086)
                     /locus_tag="ACUQR0_001641"
                     /pseudo
     CDS             complement(33885..34086)
                     /locus_tag="ACUQR0_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8470 a         8929 c         8690 g         7997 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccgga taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata atcatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcg
//
LOCUS       DBKELA010000034        33652 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.034, whole genome shotgun sequence.
ACCESSION   DBKELA010000034 DBKELA010000000
VERSION     DBKELA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.034"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..209)
                     /locus_tag="ACUQR0_001642"
     CDS             complement(<1..209)
                     /locus_tag="ACUQR0_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HHV9108737.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACUQR0_001643"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACUQR0_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HHV9108738.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACUQR0_001644"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACUQR0_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HHV9108739.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACUQR0_001645"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACUQR0_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HHV9108740.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACUQR0_001646"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACUQR0_001646"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HHV9108741.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACUQR0_001647"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACUQR0_001647"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HHV9108742.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACUQR0_001648"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACUQR0_001648"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HHV9108743.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACUQR0_001649"
     CDS             complement(4598..5320)
                     /locus_tag="ACUQR0_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9108744.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACUQR0_001650"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACUQR0_001650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHV9108745.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACUQR0_001651"
     CDS             6520..6738
                     /locus_tag="ACUQR0_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HHV9108746.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACUQR0_001652"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACUQR0_001652"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHV9108747.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACUQR0_001653"
     CDS             complement(7472..7672)
                     /locus_tag="ACUQR0_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HHV9108748.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACUQR0_001654"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACUQR0_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACUQR0_001655"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACUQR0_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HHV9108749.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACUQR0_001656"
     CDS             10101..12014
                     /locus_tag="ACUQR0_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9108750.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACUQR0_001657"
     CDS             12014..13036
                     /locus_tag="ACUQR0_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HHV9108751.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACUQR0_001658"
     CDS             13030..13248
                     /locus_tag="ACUQR0_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HHV9108752.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACUQR0_001659"
     CDS             13302..14171
                     /locus_tag="ACUQR0_001659"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HHV9108753.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACUQR0_001660"
     CDS             complement(14226..14630)
                     /locus_tag="ACUQR0_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HHV9108754.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACUQR0_001661"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACUQR0_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HHV9108755.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACUQR0_001662"
     CDS             15615..17705
                     /locus_tag="ACUQR0_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HHV9108756.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACUQR0_001663"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACUQR0_001663"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HHV9108757.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACUQR0_001664"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACUQR0_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HHV9108758.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACUQR0_001665"
     CDS             complement(19673..20275)
                     /locus_tag="ACUQR0_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HHV9108759.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACUQR0_001666"
     CDS             complement(20265..20432)
                     /locus_tag="ACUQR0_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HHV9108760.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACUQR0_001667"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACUQR0_001667"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HHV9108761.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="ACUQR0_001668"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="ACUQR0_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HHV9108762.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="ACUQR0_001669"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="ACUQR0_001669"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HHV9108763.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="ACUQR0_001670"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="ACUQR0_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HHV9108764.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="ACUQR0_001671"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="ACUQR0_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HHV9108765.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cysG"
                     /locus_tag="ACUQR0_001672"
     CDS             26649..28022
                     /gene="cysG"
                     /locus_tag="ACUQR0_001672"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HHV9108766.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="ACUQR0_001673"
     CDS             28269..28436
                     /locus_tag="ACUQR0_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HHV9108767.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="ACUQR0_001674"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="ACUQR0_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="ACUQR0_001675"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="ACUQR0_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HHV9108768.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="ACUQR0_001676"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="ACUQR0_001676"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HHV9108769.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="ACUQR0_001677"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="ACUQR0_001677"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HHV9108770.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="frlR"
                     /locus_tag="ACUQR0_001678"
                     /gene_synonym="yhfR"
     CDS             32873..33604
                     /gene="frlR"
                     /locus_tag="ACUQR0_001678"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HHV9108771.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         8095 a         8980 c         8545 g         8032 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggtaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct ctcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agagcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct agcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acaagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacattacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aagaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cg
//
LOCUS       DBKELA010000035        33129 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.035, whole genome shotgun sequence.
ACCESSION   DBKELA010000035 DBKELA010000000
VERSION     DBKELA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.035"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(2..94)
                     /locus_tag="ACUQR0_001679"
     tRNA            complement(2..94)
                     /locus_tag="ACUQR0_001679"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACUQR0_001680"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACUQR0_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HHV9108772.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACUQR0_001681"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACUQR0_001681"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HHV9108773.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACUQR0_001682"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACUQR0_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HHV9108774.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACUQR0_001683"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACUQR0_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HHV9108775.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACUQR0_001684"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACUQR0_001684"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HHV9108776.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACUQR0_001685"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACUQR0_001685"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HHV9108777.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="ACUQR0_001686"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="ACUQR0_001686"
                     /inference="COORDINATES: protein motif:HMM:NF015565.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="ACUQR0_001687"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="ACUQR0_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HHV9108778.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="ACUQR0_001688"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="ACUQR0_001688"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HHV9108779.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="ACUQR0_001689"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="ACUQR0_001689"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HHV9108780.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10291..10602
                     /gene="gutM"
                     /locus_tag="ACUQR0_001690"
     CDS             10291..10602
                     /gene="gutM"
                     /locus_tag="ACUQR0_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HHV9108781.1"
                     /translation="MALGGWQISRFNRAFDTLCQQGRVGVGRSSGRFKPRVVVAIALD
                     DQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPHDPLSQNALSLALKLKR
                     G"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="ACUQR0_001691"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="ACUQR0_001691"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HHV9108782.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="ACUQR0_001692"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="ACUQR0_001692"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HHV9108783.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACUQR0_001693"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACUQR0_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HHV9108784.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="ACUQR0_001694"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="ACUQR0_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HHV9108785.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="ACUQR0_001695"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="ACUQR0_001695"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HHV9108786.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACUQR0_001696"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACUQR0_001696"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HHV9108787.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACUQR0_001697"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACUQR0_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HHV9108788.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACUQR0_001698"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACUQR0_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HHV9108789.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20948..21019)
                     /locus_tag="ACUQR0_001699"
                     /pseudo
     CDS             complement(20948..21019)
                     /locus_tag="ACUQR0_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACUQR0_001700"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACUQR0_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HHV9108790.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACUQR0_001701"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACUQR0_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HHV9108791.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25521)
                     /gene="hycC"
                     /locus_tag="ACUQR0_001702"
                     /pseudo
     CDS             complement(23682..25521)
                     /gene="hycC"
                     /locus_tag="ACUQR0_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="ACUQR0_001703"
     CDS             complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="ACUQR0_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HHV9108792.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="ACUQR0_001704"
     CDS             complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="ACUQR0_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HHV9108793.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26927..27277
                     /gene="hypA"
                     /locus_tag="ACUQR0_001705"
     CDS             26927..27277
                     /gene="hypA"
                     /locus_tag="ACUQR0_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HHV9108794.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27281..28153
                     /gene="hypB"
                     /locus_tag="ACUQR0_001706"
     CDS             27281..28153
                     /gene="hypB"
                     /locus_tag="ACUQR0_001706"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HHV9108795.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28144..28416
                     /gene="hypC"
                     /locus_tag="ACUQR0_001707"
     CDS             28144..28416
                     /gene="hypC"
                     /locus_tag="ACUQR0_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HHV9108796.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28416..29537
                     /gene="hypD"
                     /locus_tag="ACUQR0_001708"
     CDS             28416..29537
                     /gene="hypD"
                     /locus_tag="ACUQR0_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HHV9108797.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29534..30544
                     /gene="hypE"
                     /locus_tag="ACUQR0_001709"
     CDS             29534..30544
                     /gene="hypE"
                     /locus_tag="ACUQR0_001709"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HHV9108798.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30618..32696
                     /gene="flhA"
                     /locus_tag="ACUQR0_001710"
     CDS             30618..32696
                     /gene="flhA"
                     /locus_tag="ACUQR0_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HHV9108799.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32733..33086)
                     /locus_tag="ACUQR0_001711"
     CDS             complement(32733..33086)
                     /locus_tag="ACUQR0_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HHV9108800.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7807 a         9232 c         8622 g         7468 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gggaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctagcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccagttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtacattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat cccgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcagg ataatgccga tattttccag ggtgtggtaa
    24661 gccagcaggc gctggatgtt gtgttccatc aacgcataca gaccaccgac gaacgcggtg
    24721 atcatgccga gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt
    24781 gacagggtta acatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca
    24841 gctggcgcag aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatccca
    24901 gccagcaggc caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt
    24961 tgcatacgca tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag
    25021 caagcaatca ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct
    25081 ttgctgttgc tggtgaggaa caccgcacac aaggccatga cttcggccat taccacgaac
    25141 atgccgaggt tgctggcgat gacggcgcag acggcggcag ccatcaacat attgatctgc
    25201 aagccgttgc acttcacctg cgcgtggcga tgccagtcaa tgttatagag gctgacaaac
    25261 agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa gcggggagat ttgcacatcg
    25321 tagcttacca gcgacagcgc accgctcacg ccaaccgcgc cagtcagtac agtgaagccc
    25381 gcggctgccg tatacagact accaaccgcg ccgccaattc cggctatcca gccacttagc
    25441 gctttttgaa aagagaaatg ccagaacagc ggcggcgaca aaccatgcca cgccgctatt
    25501 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    25561 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    25621 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacacaggc cggaccttgt
    25681 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    25741 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    25801 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    25861 atgccgcaca gtttgcagct tacgcacaga ctttcattca actgcacggc cccatcaacg
    25921 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    25981 agctgcggcg cagattcttt ttcattgagc atcactctca ggcgcggcat tgattgcagg
    26041 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg tgtggcagcc gatacagagc
    26101 gtggagtcgg caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    26161 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    26221 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    26281 cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac
    26341 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    26401 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    26461 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    26521 gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    26581 ggcgtgatac aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    26641 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    26701 ttcccaaata gtcattgtca ggttactcgt ttaacagaga tgccagcttt aagcattttt
    26761 tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat gaatttcgtc
    26821 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
    26881 tggcgcaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    26941 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    27001 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    27061 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    27121 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    27181 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    27241 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    27301 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    27361 cgcgccattt gccccggcgg cacgcccgca gatgaaaatc accggcatta aagcgcctga
    27421 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    27481 tcacacaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    27541 aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    27601 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    27661 cctgaaagac agcgttccgt gcgcggttat tgaaggcgac cagcaaaccg tgaacgatgc
    27721 cgcacgcatt cgcgctaccg gtacaccagc gattcaggtg aacaccggta aaggctgcca
    27781 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
    27841 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    27901 acacaaagta gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    27961 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatctcaa
    28021 ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    28081 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    28141 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    28201 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    28261 ggtcagccgc gcgtgggcca gtgggtactg gttcacgttg gctttgccat gagcgtaatt
    28321 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    28381 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    28441 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    28501 acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc cacgccattt
    28561 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    28621 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
    28681 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa cagggatcgc
    28741 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    28801 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    28861 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    28921 tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg ctggaacagc
    28981 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    29041 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    29101 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    29161 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    29221 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    29281 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    29341 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggtgag gtattaacgg
    29401 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
    29461 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    29521 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    29581 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    29641 agatcaggca cgtcttgatc tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc
    29701 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
    29761 gatttgcggc accgcgaatg acgttgcggg cagtggcgct attccgcgct atctctcctg
    29821 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    29881 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    29941 gcgcggcgca gcagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
    30001 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta gcggtacact
    30061 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    30121 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    30181 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    30241 agcctgcggt tgtggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    30301 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    30361 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    30421 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    30481 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    30541 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    30601 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    30661 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    30721 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    30781 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
    30841 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    30901 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    30961 ccaaatttgg tcactattgc ctgatgccac tggcggcggg agggcatatt tttggtggct
    31021 gtgaatttat tcgttatgac gagcgtccct ggagcgaaaa agagttcaat cgtctgcaaa
    31081 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gtcaacaatg
    31141 tcgactatga gttgttatgc cgggagcgcg ataacttccg catcctggtt gccattacca
    31201 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    31261 attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac
    31321 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
    31381 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    31441 atctctacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    31501 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    31561 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc
    31621 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    31681 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    31741 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    31801 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggctcgggta
    31861 aagagctgat tgcccgtgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
    31921 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
    31981 gtggggcttt taccggtgcc agcgcccagc gcattggtcg ttttgaactg gcggataaaa
    32041 gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    32101 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
    32161 tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
    32221 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
    32281 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    32341 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
    32401 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc agtattgcta acacgcggca
    32461 acgtgctgca gctgtcattg ccagatattg ctttaccgga gcctgaaacg ccgcctgccg
    32521 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag
    32581 aaaccaacag cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    32641 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    32701 aaattgccgg acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat
    32761 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    32821 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    32881 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    32941 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    33001 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    33061 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgc atttatatta
    33121 catcttaat
//
LOCUS       DBKELA010000036        32634 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.036, whole genome shotgun sequence.
ACCESSION   DBKELA010000036 DBKELA010000000
VERSION     DBKELA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.036"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..956)
                     /locus_tag="ACUQR0_001712"
     CDS             complement(<1..956)
                     /locus_tag="ACUQR0_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HHV9108801.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACUQR0_001713"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACUQR0_001713"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HHV9108802.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACUQR0_001714"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACUQR0_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACUQR0_001715"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACUQR0_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HHV9108803.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACUQR0_001716"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACUQR0_001716"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HHV9108804.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACUQR0_001717"
     CDS             complement(5018..6346)
                     /locus_tag="ACUQR0_001717"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HHV9108805.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="ACUQR0_001718"
     CDS             complement(6354..8702)
                     /locus_tag="ACUQR0_001718"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HHV9108806.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="ACUQR0_001719"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="ACUQR0_001719"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HHV9108807.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="ACUQR0_001720"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="ACUQR0_001720"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HHV9108808.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="ACUQR0_001721"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="ACUQR0_001721"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HHV9108809.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="ACUQR0_001722"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="ACUQR0_001722"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HHV9108810.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="ACUQR0_001723"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="ACUQR0_001723"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HHV9108811.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="ACUQR0_001724"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="ACUQR0_001724"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HHV9108812.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="ACUQR0_001725"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="ACUQR0_001725"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HHV9108813.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="ACUQR0_001726"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="ACUQR0_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HHV9108814.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="ACUQR0_001727"
     CDS             18273..19172
                     /locus_tag="ACUQR0_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HHV9108815.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="ACUQR0_001728"
     CDS             19178..21610
                     /locus_tag="ACUQR0_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HHV9108816.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="ACUQR0_001729"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="ACUQR0_001729"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HHV9108817.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="ACUQR0_001730"
     CDS             22723..23988
                     /locus_tag="ACUQR0_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HHV9108818.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="ACUQR0_001731"
     CDS             complement(24229..25821)
                     /locus_tag="ACUQR0_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HHV9108819.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="ACUQR0_001732"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="ACUQR0_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHV9108820.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="ACUQR0_001733"
     CDS             complement(27018..28136)
                     /locus_tag="ACUQR0_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HHV9108821.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="ACUQR0_001734"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="ACUQR0_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HHV9108822.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="ACUQR0_001735"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="ACUQR0_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HHV9108823.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="ACUQR0_001736"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="ACUQR0_001736"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HHV9108824.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="ACUQR0_001737"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="ACUQR0_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HHV9108825.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="ACUQR0_001738"
     CDS             complement(31385..31450)
                     /locus_tag="ACUQR0_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9108826.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="ACUQR0_001739"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="ACUQR0_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HHV9108827.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8167 a         8699 c         7884 g         7884 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgtggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatacagaa tttctcgcta cttttcctct acac
//
LOCUS       DBKELA010000037        32127 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.037, whole genome shotgun sequence.
ACCESSION   DBKELA010000037 DBKELA010000000
VERSION     DBKELA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.037"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(91..627)
                     /gene="ssb1"
                     /locus_tag="ACUQR0_001740"
     CDS             complement(91..627)
                     /gene="ssb1"
                     /locus_tag="ACUQR0_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HHV9108828.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            881..3703
                     /gene="uvrA"
                     /locus_tag="ACUQR0_001741"
     CDS             881..3703
                     /gene="uvrA"
                     /locus_tag="ACUQR0_001741"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HHV9108829.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3738..4094)
                     /locus_tag="ACUQR0_001742"
     CDS             complement(3738..4094)
                     /locus_tag="ACUQR0_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HHV9108830.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4098..4514)
                     /locus_tag="ACUQR0_001743"
     CDS             complement(4098..4514)
                     /locus_tag="ACUQR0_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HHV9108831.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="ACUQR0_001744"
     CDS             complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="ACUQR0_001744"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HHV9108832.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5740..5944
                     /locus_tag="ACUQR0_001745"
                     /pseudo
     CDS             5740..5944
                     /locus_tag="ACUQR0_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6447..7640)
                     /gene="tyrB"
                     /locus_tag="ACUQR0_001746"
     CDS             complement(6447..7640)
                     /gene="tyrB"
                     /locus_tag="ACUQR0_001746"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HHV9108833.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7893..8972)
                     /gene="alr"
                     /locus_tag="ACUQR0_001747"
     CDS             complement(7893..8972)
                     /gene="alr"
                     /locus_tag="ACUQR0_001747"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HHV9108834.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9025..10440)
                     /gene="dnaB"
                     /locus_tag="ACUQR0_001748"
     CDS             complement(9025..10440)
                     /gene="dnaB"
                     /locus_tag="ACUQR0_001748"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HHV9108835.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10523..11506
                     /locus_tag="ACUQR0_001749"
     CDS             10523..11506
                     /locus_tag="ACUQR0_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HHV9108836.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11672..11914)
                     /gene="pspG"
                     /locus_tag="ACUQR0_001750"
     CDS             complement(11672..11914)
                     /gene="pspG"
                     /locus_tag="ACUQR0_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HHV9108837.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12048..13085)
                     /gene="dusA"
                     /locus_tag="ACUQR0_001751"
     CDS             complement(12048..13085)
                     /gene="dusA"
                     /locus_tag="ACUQR0_001751"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HHV9108838.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13448..14433)
                     /locus_tag="ACUQR0_001752"
                     /pseudo
     CDS             complement(13448..14433)
                     /locus_tag="ACUQR0_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105478490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14751..15266
                     /gene="zur"
                     /locus_tag="ACUQR0_001753"
     CDS             14751..15266
                     /gene="zur"
                     /locus_tag="ACUQR0_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HHV9108839.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15308..15517)
                     /locus_tag="ACUQR0_001754"
     CDS             complement(15308..15517)
                     /locus_tag="ACUQR0_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HHV9108840.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15633..16958)
                     /gene="dinF"
                     /locus_tag="ACUQR0_001755"
     CDS             complement(15633..16958)
                     /gene="dinF"
                     /locus_tag="ACUQR0_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HHV9108841.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17031..17639)
                     /gene="lexA"
                     /locus_tag="ACUQR0_001756"
     CDS             complement(17031..17639)
                     /gene="lexA"
                     /locus_tag="ACUQR0_001756"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HHV9108842.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17749..18117)
                     /gene="dgkA"
                     /locus_tag="ACUQR0_001757"
     CDS             complement(17749..18117)
                     /gene="dgkA"
                     /locus_tag="ACUQR0_001757"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HHV9108843.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18288..20711
                     /gene="plsB"
                     /locus_tag="ACUQR0_001758"
     CDS             18288..20711
                     /gene="plsB"
                     /locus_tag="ACUQR0_001758"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HHV9108844.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20866..21738)
                     /gene="ubiA"
                     /locus_tag="ACUQR0_001759"
     CDS             complement(20866..21738)
                     /gene="ubiA"
                     /locus_tag="ACUQR0_001759"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HHV9108845.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21751..22248)
                     /gene="ubiC"
                     /locus_tag="ACUQR0_001760"
     CDS             complement(21751..22248)
                     /gene="ubiC"
                     /locus_tag="ACUQR0_001760"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HHV9108846.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22471..24051)
                     /locus_tag="ACUQR0_001761"
                     /pseudo
     CDS             complement(22471..24051)
                     /locus_tag="ACUQR0_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24280..25200)
                     /gene="malM"
                     /locus_tag="ACUQR0_001762"
     CDS             complement(24280..25200)
                     /gene="malM"
                     /locus_tag="ACUQR0_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HHV9108847.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25352..26692)
                     /gene="lamB"
                     /locus_tag="ACUQR0_001763"
     CDS             complement(25352..26692)
                     /gene="lamB"
                     /locus_tag="ACUQR0_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HHV9108848.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26764..27879)
                     /gene="malK"
                     /locus_tag="ACUQR0_001764"
     CDS             complement(26764..27879)
                     /gene="malK"
                     /locus_tag="ACUQR0_001764"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HHV9108849.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28244..29434
                     /gene="malE"
                     /locus_tag="ACUQR0_001765"
     CDS             28244..29434
                     /gene="malE"
                     /locus_tag="ACUQR0_001765"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HHV9108850.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29588..31132
                     /gene="malF"
                     /locus_tag="ACUQR0_001766"
     CDS             29588..31132
                     /gene="malF"
                     /locus_tag="ACUQR0_001766"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HHV9108851.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31147..32037
                     /gene="malG"
                     /locus_tag="ACUQR0_001767"
     CDS             31147..32037
                     /gene="malG"
                     /locus_tag="ACUQR0_001767"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HHV9108852.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8173 a         8247 c         7783 g         7924 t
ORIGIN      
        1 agcctccata acggaggctt cttttttatg gctggcagat gctttaatca tccaccttaa
       61 aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat caaagtccat
      121 cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact gcgcgccgcc
      181 gctgaactga ttgccaccct gcggctgctg aggttgaccc caaccgccct gcggctgacc
      241 accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca gcatctgcat
      301 ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg attgatcggt
      361 ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac gcagatattc
      421 gctcgccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag tctgttcttt
      481 catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa tgttggcaac
      541 tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac caacgagaat
      601 aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc gttctgaata
      661 agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt gacctcggtt
      721 ccgggaaaca aacctggcca gacattgtta cacaacactc cggataatgc attccaatac
      781 tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc
      841 atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga tcgaagttcg
      901 gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg acaagctcat
      961 tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca ccttatatgc
     1021 cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt ttctgtcact
     1081 gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct caattgagca
     1141 gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa tccacgacta
     1201 tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg acgtcccgct
     1261 ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg aaggcaagcg
     1321 tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca aaacgctgga
     1381 gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct gcgatctttc
     1441 cgatccgccg aaactggaac tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt
     1501 caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg cgctggaact
     1561 ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag agctgctgtt
     1621 ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg agccgcgact
     1681 gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg gcgtacagca
     1741 atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg gcggtgcgat
     1801 ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc tggcagatca
     1861 ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc ataaagtggt
     1921 gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc gtggcgatac
     1981 ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc gttataaaga
     2041 gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc gcccatgcgc
     2101 cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc
     2161 gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca acaatctcaa
     2221 actcgctggt cagcgggcga agattgcgga aaaaattctt aaagagatcg gcgatcgcct
     2281 gaaattcctc gtaaacgtcg gcctgaatta cctgacactt tcccgctcgg cagaaacact
     2341 ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg gcctggttgg
     2401 cgttatgtac gtgctggacg agccgtctat cggcctgcac cagcgcgata acgagcgcct
     2461 gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg tggagcatga
     2521 cgaagacgca attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg cgggtgttca
     2581 cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc ctgaatcgtt
     2641 gaccgggcag tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac gcgttccggc
     2701 gaatccagaa aaagtgctga agctgacagg cgcacgcggc aacaacctga aggacgtgac
     2761 gctcacgctg ccagtcggtc tgtttacctg catcacaggg gtttcaggtt ccggtaaatc
     2821 gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat
     2881 cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgata aagtgatcga
     2941 tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct ataccggcgt
     3001 gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc gtggttatac
     3061 gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc agggcgacgg
     3121 tgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg atcagtgcaa
     3181 aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa ccatccacga
     3241 agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc cagctctggc
     3301 gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg ggcagtccgc
     3361 aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg
     3421 cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc acttcgccga
     3481 tattcagcaa ctcctcgacg tgctgcataa actgcgcgat cagggcaata ccattgtggt
     3541 gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc tgggaccaga
     3601 aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg tcgcggagtg
     3661 cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa ggccgctttc
     3721 tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc cggtagtaaa
     3781 ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc cggcagcgaa
     3841 ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggctagg acgcacatcg
     3901 ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga aacagctggg
     3961 cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat ctgcgtcgct
     4021 ttccagtcgt tatgcacgct ctgttctgcg cttggttttg ccatgcaata ttgtagcaac
     4081 tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg
     4141 tggatgcgat gttcccccag ccagatgcct tgccaagtgc cggtctgaat acgcccttta
     4201 tgtaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga aggcatatcg
     4261 tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg gaggaaaaaa
     4321 cactccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag agaggcggag
     4381 gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc agccagctga
     4441 ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc
     4501 ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg cagcaagaca
     4561 gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcatcac tctctcccgc
     4621 tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct tgtggcaagg
     4681 gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg
     4741 cggcggtaat atcgttatcg gaatcgccat aaaaaattcg gatattttta tcctgcagcc
     4801 attgcgattt tgtattttgc cctggtttat cgcccgcaaa gattaccgga ttcatgttgg
     4861 tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt ttcgtcgggc
     4921 tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca atcagctggc
     4981 gagcgacctc ttttggaatg ctgaattcat cccagccatt attcattttt tcccagaaca
     5041 caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg cgccagaatc
     5101 ccggactgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc
     5161 ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc tgttcagcaa
     5221 gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg
     5281 aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat
     5341 ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg caaaaaccat
     5401 ctgaccataa cgccagctgc cggggaaagg aaggattatc tgcggttttt gtgatgaaaa
     5461 gcaggatgat tatatttaac ccagtaaata cgttcctgtc tttttttgta taaaaaatgt
     5521 ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc ctcggcaaat
     5581 gagcaaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa tgatacagca
     5641 ttacaaaaac aatcgaagtt tataaagatg atttctgatt gaccagcccc ctaccctaca
     5701 atggtgatta attttattac tcgcgatgga gatgatgata tgaatatatc atatgtgaat
     5761 agcaacaaaa cgacatcttt acctgttgag ctagatgcac taaataataa agatatctct
     5821 tatgccaaag atttcttttt atacatagaa acacagttaa aaattgcaaa agatttttgt
     5881 agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc atttattgat
     5941 ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct ttgctgtttt
     6001 gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga tattggtaat
     6061 caatataagg ttaaacttac acaaagcgga aaaaagagtt ctctttaaca ttagaattta
     6121 acgatactat aattgaatct cagaaagtca caggcaataa ggcaaagcat attcttgagg
     6181 atatagaaat attctatcgt aacaaacccg atacttatta ttaaatttaa taatggttag
     6241 atgtgcagaa attattactc cacacgaaaa aatagggaaa ggaattccag ataattggga
     6301 taaacttaac aataaaagaa ctgacaacgt tttattatct ctcacgtaga acaacggcat
     6361 caaaaaaatc atctacaaag aaatagtgaa agcgacgaca gtttaatttc actgcaggct
     6421 gggtaactcc agcctgcttt cctgcattac atcaccgcag caaacgcctt tgccacacgt
     6481 tgtacatttg ccgtatttaa cccggcgaca cacatgcgac cgctggcgat gagatagaca
     6541 ccaaattctt cacgtagtcg gtcaacctga gcggcactta aaccggtata actgaacatg
     6601 ccgcgctgat taagcagata atcgaaattg cgttctggca tctctgtgct taataccttc
     6661 accaattcct gacgcattgc cagaatgcga gtacgcatct cttctacttc cgccagccag
     6721 ctggctttca atgcctcgtc attcagcact gcagccacca cctgcgcacc aaaattcggc
     6781 gggctggagt agttgcggcg aactgttgct ttcaattgcc ccagtacgcg gccagcggct
     6841 tcggcatctt cacacagaac agaaagtccg ccgacgcgct cgccgtaaag ggagaaaatt
     6901 ttcgagaacg aattgctcac cagagcgggt aatccagcgc tggcaatggc gcgaatagcg
     6961 taggcatcct cttccatacc ggcactaaat ccttgatagg caatatcgag gaatgggata
     7021 agctcgcggg ctttgagaat ttcaatcacc gcatcccact gatcattagt gagatcggca
     7081 cccgttgggt tgtggcaaca tggatgcagc aacacaatac tgcgggcagg taatgttttc
     7141 agcgtcgcca acaggtcatt aaagcgcacg ctgttagtca cttcgtcata ccaggggtaa
     7201 gtactcactt cgaatccagc cccggcgaat attgctacgt ggttttccca agtaggatcg
     7261 ctgacccaga cgcctgattc cgggaagtag cgtttcagga aatccgcgcc cactttcaat
     7321 gcccctgagc cgccaagggt ttgaatggtt gctacgcgct gttgttgcag taccggatgg
     7381 tccgcaccaa acagcagcgg cgcaatggca tggcgatagc tgttaagccc ttccatcggt
     7441 aaataaagcg aagcgccatg gggctgcgca ttcaggcgcg cttccgcctc cgccacggct
     7501 ttcaattgtg gaataattcc gtcttcgttg tagtacagac cgatacttaa attcactttg
     7561 tcgctgcgag ggtcttcttt aaaacgctcc ataagcgtaa gaatcgggtc gccagcgtag
     7621 gcgtcaactt tttgaaacac gcgatggttc tccaggttta cgggcaggtg gttaaaacac
     7681 aataaaccgg aagaaggcga agatcgagtg gatgttcagg agcgaacggc aattagcaac
     7741 agagtgagac taatgataaa cgtacatccg ccagaggcac gacctttata agagcatgaa
     7801 agaacatcaa cttattgaat ttttaggatt ttattggccg gataaggcat tcacgccgca
     7861 tccggcacag acaatcaaat attacagaac gattaatcca cgtatttcat cgcgaccctt
     7921 gaagtcaggc gcgtaataag ttcgtaagcg cttactttcg tcatttcagc gatacgttct
     7981 acgggcaaac cttcgcccca taaaatgacc ggatccccgg ctttgtcctg cgcctgtgga
     8041 cctaagtcta cgcagatcat atccatcgcc acgcgcccga caatcggtac ttcgcgaccg
     8101 ttcaccagca ctggcgtacc ggacggcgcg gcgcgcggat aaccatcgcc atagcccatc
     8161 gcgactacgc caagacgggt atcacgttcg cttacccagg ttccaccata accaacaggc
     8221 tctccggctt tatgctcacg cacggcaatc aggctggagg ttagtgacat cactggctga
     8281 cagccaaaat cggcaccggt ggagcgatct tccagcggcg agacgccata aagaatgatg
     8341 cccgggcgca cccagtcaaa atgcgactgt ggccacagca gaatgccacc cgacgcggca
     8401 atggaacgtt gaccaggttt gccttcgcaa aaggtattaa agatagcgag ttgtttctcg
     8461 gttgcgccac attttggttc atccgcgcgc gcaaaatggc tgacgatatt caccggctga
     8521 cgaacgtttt tgcactgggt caggcgatga taaaacgcct cagcctgttc cggccttacg
     8581 cccagacggt gcataccggt atcgagtttc atccagacgg taaccggctc gtccaagcta
     8641 gcctcttcca gcgcagccag ctgttcttcg ttatgcacgg cggtatgaaa atgttgcgca
     8701 gaaatcgtcg gcagatctct ggcatcaaaa aagccttcga gtaacagtac aggtttggtg
     8761 attccccccg cacgcagtcg cagagcttct tcgagacggg ctacgccaaa ggcgtcagca
     8821 tcggggagcg ttcgcgcggt ctcaagaaga ccgtgaccat aagcgttcgc tttcaccacc
     8881 gcaaccattt tactggcagg tgccagttca cgaagacgtt gcaggttgtg tcgcagagcg
     8941 cggcggttaa tcacaacagt tgccgcttgc atttgtgttc cttgataatt gtttgcttta
     9001 attacctaat tcataaaata attattattc gtcgtcgtac tgcggccccg catagttgtc
     9061 gaagcgcgac cattgaccgt taaaggtcag gcgtaccgtc ccgattgggc cgttacgttg
     9121 tttaccgata ataatttccg cgatgccttt taaatcactg ttttcgtgat acacctcatc
     9181 acgatagata aacatgatca agtccgcatc ctgctcgata gagccagatt cacgcaggtc
     9241 ggagttgacc ggacgtttgt cggcacgttg ttccagagaa cggttcaact gggacagcgc
     9301 caccaccggc acgttcagtt ctttcgccag tgctttcagc gagcgggaga tttctgcaat
     9361 ttccagcgta cggttatcgg aaagcgccgg tacgcgcatc agttgcaggt agtcgatcat
     9421 gataagcccg atgccgccgt gttcacgggc aatacggcgt gcgcgggaac gcacttccgt
     9481 tggcgtcagg ccggaagagt catcgatata gatattgcgt ttttcgagca aaatacccat
     9541 ggtgccggaa atgcgcgccc agtcttcatc atcgagctgc ccggtacgga ttttagtctg
     9601 gtcaacgcgc gacagcgacg ccagagaacg catcatgatc tgttctgatg gcatctccag
     9661 cgagaagata agtactggtt tatcctgcaa cattgccgcg ttttcgacga ggttcatcgc
     9721 aaatgttgtt ttacccatcg acggacgcgc ggcgacgatg atcaaatccg acggctgcaa
     9781 gccagcggtt tttttgttga gatcgtcata accggtgttt accccggtaa cgccatcgtg
     9841 tggctgctga aacaactgct caatacgcgc cacggttgcg tcgagcacat cggcgatgtt
     9901 cttcggccct tcgtctttgt ttgcacgact ttcggcaatt ttaaagacgc gggattcagc
     9961 aaggtccagc agatcttcgc tggtacgccc ctgcggatca aaaccggctt cagcaatctc
    10021 attcgcaacc gagatcatct cacggacaac ggcacgttca cgcacgatgt ccgcataagc
    10081 actgatgttc gccgcacttg gcgtattttt tgacagctct gccagataag caaaaccacc
    10141 gacgctatcg agttgcccct ggcgttccaa cgattccgca agggtaatca gatcgatagg
    10201 actaccgctt tcctgcaaac gcgccatttc agtaaagata tgacggtgtg ggcgggtgta
    10261 aaaatcgtct gctaccacac gctcggctac atcatcccag cgttcgttat ccagcattaa
    10321 accgcccaac accgactgct ccgcttcgat cgagtgcgga ggcactttca gcccggcaac
    10381 ttgtggatcg cgttcgcggg gttcagcctg ctgtttgttg aagggtttat ttcctgccat
    10441 agtgaatgga gttaccgaaa taaagaatgg gtcgaaactt taccatatga agcagaccct
    10501 gacgatacgt tctggaggac acatggcaac acgaattgaa tttcacaagc acggtggccc
    10561 ggaagtactt caagccgtag agttcactcc tgccgatccg gcggagaatg aaatccaggt
    10621 cgaaaataaa gccatcggca tcaattttat cgacacgtat atccgcagcg gcctttaccc
    10681 gccgccatcg ctacccagcg gattaggtac cgaagcggca ggcatcgtga gtaaagtcgg
    10741 cagtggtgta aagcatatta aggctggcga tcatgtagtc tatgcgcagt cggcgttagg
    10801 cgcttacagc tctgtgcata acattaatgc ggataaagcg gcgattctgc ctgcggcaat
    10861 ttcttttgag caagctgcgg catccttcct gaaaggctta acggtttatt atctgctgcg
    10921 caaaacctat gaaattaaac ccgatgaaca gttcctgttc cacgcagcgg ctggcggcat
    10981 tggcttaatt gcctgccagt gggcaaaagc cctgggcgcg aaactcatcg gcaccgtagg
    11041 aaccgcgcaa aaagcgcaga gcgcgctaaa agccggcgcg tggcaggtta ttaactatcg
    11101 tgaagagaat ctggtcgagc gattgaaaga gatcaccggc ggtaagaaag tgcgcgtagt
    11161 gtacgattcc gtgggcagag acacctggga acggtcgctg gattgcctgc aacgccgcgg
    11221 cttaatggtc agttttggca actcatcagg tgcggttacc ggtgtgaact taggcattct
    11281 caatcaaaaa ggctcgttgt atgtgacacg cccttccctg caaggctata tcaccacgcg
    11341 ggaggaatta accgaggcca gtaatgaact gttctctttg attgccagcg gtgtgattaa
    11401 ggtcgatgtc gccgagcagc agaaatatcc gctgaaggat gcgcagcgtg cgcatgagat
    11461 tctggaaagc cgggcgacgc aaggttccag cctgctgatt ccataaaaga aatagggctt
    11521 ccacctggga agccctttct ttttatagtt cggctgtatg tagggtacag cacgatgaat
    11581 ctgttagagg cgcaatagtg acagatttga ttatcaattc ctattttgtt ctaaggataa
    11641 aaccttaggt tgtgatcatc cgcacaatcc cttagtaacg ccagcggtca taacgctgat
    11701 atttcggcat ttttggtgct ttaatcgcct taataaccca caccaccgca atcgccagta
    11761 gtaaccacgg cagcaactta atcatcaatg ccagcatacc gccgaggaac ataatggccg
    11821 tcgccacaac cagcgcggcg ataatgccca gcaacgaaac gccggtgacc atcagcatga
    11881 caaaaaagcc aatcacaaaa agtagttcca gcatgatgct ctcccaaata tgaaatctct
    11941 tgctggcatt acaagaatca tgccaaaaat aatctattga tttaacagca aaacgccccg
    12001 cgacggtgcg cagggcgtgg tgaatttgac tactttttgg tgaaaagtta acgcttatcc
    12061 gccaccagtt tgagcgcgtg ttccagcacg ttaatgtcag cacccgcttt atgggcattt
    12121 tcacttaaat aacgccgcca ctgccgcgcg ccaggaatac cctggaacaa acccagcata
    12181 tgccggataa tatggccgag atacgtcccc tggctgagtt catgctcaat gtacggatac
    12241 atggcacgca ctactgccac cggatcggca tcggtatccg aggaaccaaa gatctcccgg
    12301 tctaccgccg ccagaatacc cggattctga tacgcctcgc gcccgaccat cacgccatcc
    12361 atatgttgca aatgcgcttt agcttcttcc agcgacttga taccaccgtt aatcgacatt
    12421 gtcagatgcg ggaagtcacg cttcagttga tacacacgcg gataatcgag cggcgggatt
    12481 tcacggtttt ctttcgggct taaccccgaa agccaggctt tacgtgcgtg gatgatgaac
    12541 atctcacact cgcctttgcc ggaaacggta ttgatgaaat cgcagagaaa ttcatagctg
    12601 tcctggtcgt cgatgccaat acgcgttttc accgtcaccg gaatcgacac cacatcgcgc
    12661 atcgctttca cgcagtcggc aaccagctgc gcattaccca tcagacacgc accaaacatg
    12721 ccgttctgca cccggtcaga cgggcagccg acattcaggt tgatctcatc atatccgcgc
    12781 gcttctgcca gctttgcaca ctgcgccagc gccgccggat cgctaccgcc cagttgcaac
    12841 gctaccggat gttcttcttc actgtacgcc agataatcac ctttaccgtg aataatcgcc
    12901 cctgtggtca ccatttcggt ataaagcaac gtattgcggg aaagcagacg caagaaatag
    12961 cggcaatgtc tgtccgtcca gtcgagcatt ggtgcaacac taaaccgacc actccagtga
    13021 acgtcagttt tttcaggcat tgcgctggtt tggttgattt tttgcatttc agaattaccg
    13081 tgcattttca aatgtagaga ttattttatc gatatatcat ggggttatgt tattcagctt
    13141 cactgttcag gaggctcaat agcggggtac tataccataa caacaggaag cgcctgtctc
    13201 attgcaaaag aaaattgaga tcctctcaag gcatgaagct ctcacgaagt gatggaaata
    13261 atcttattag ccgttaactt tgttaaggcc aatgataaac aatccaggtt cgacgataaa
    13321 taaaaaatca cacattaaac tctggtgata tatctccctg ctaatagcat tgatagagaa
    13381 aaaagaaccc aataaatatt gggtcctttt atataatgcc ttccatacta tcgaagaact
    13441 tcacatatta tttctccgat ttaaccccga acaagtcata aattaattta gaagtgtctg
    13501 taagtatttt aatctcttcc tttgaggttg ggtcaaacga cttcgcgaag ctaattaatt
    13561 gtggtgcagc atctcgcatt ttgcttaaaa tatcaggatc gagcgttcct tcattcacca
    13621 gatgtgacat cttatccaga tagccatcaa aattcatttc tcccccatct gccagacgct
    13681 tcatctctcc caggtacttc ttcatatcac gttgatataa tttttcaaac tgagctttca
    13741 ggtagctctc attattttca ttaatataca ctgtttctga taaatctgcg taggcactag
    13801 aatatgaaag tgaaattaac agcgaagcaa ataattttaa gctgttgtca tcatgttcct
    13861 ggcaggcatt aacaaacgtt aaaaaaccag aacctattgc ttgaaaatgt atattcgcta
    13921 aaggttcatt atccttagat tcctcataaa atagctgaac cgtggaggga aggccattct
    13981 tagtttctac atcaaaagta cattgttcaa tgggcattgc atctttatta tccccaggat
    14041 tacatatact attaatcgcg aaatgaaaaa tagcccggtc aacaactgat gcaataaagt
    14101 cacgatcgtt taaatcctca tcagtgcaat cgtcagtata cttattgacc cacattttga
    14161 gatcatttat gtgctcaatt aaaaacacat ttttttacgt gcatgccata aattatgcgt
    14221 gattcgttga ataaacaaat ctatttttgc aataattata tcccgatgac gcagcgctgc
    14281 ataatcaata gatttaccat atatattatt taatttacct ttgattgaac ctctattatc
    14341 caatttatct ttggttgcat ctttattttc gaattgattt gaattgagtg ttttattaaa
    14401 gtatccagtt gcaccgggta ttatcttcaa catgattcat ctaccttttt tgtcgtaccg
    14461 tgtcctcctt ataattcttt tgccatctgg tcactatcaa aaatcgctca taatttctga
    14521 actacaggta tgcgtttaaa cccagttaaa taaatagcga gttaacttga agcaatttat
    14581 ttaaaaatga attattaatt attatcgtgt tgataatggt acgacaaggc aacacaacac
    14641 cttacctaca aaacagaaaa tcaacattac ggcaacaata agggttctcg gtgtttttcc
    14701 tggaacatgg taaagtaagg acattcttaa cccccacttt gaggtgcccg atggaaaaga
    14761 ccacaacgca ggagttatta gcgcaggctg aaaaaatctg cgcgcagcgt aatgtgcgcc
    14821 tgaccccaca gcgcctggaa gtgttgcgcc tgatgagtct gcaggatggc gctatcagcg
    14881 cttatgatct gcttgattta ctgcgcgaag ctgaaccgca agccaagccg ccaacggttt
    14941 atcgcgcgct ggattttctg cttgagcaag gttttgtgca taaggtggaa tccaccaaca
    15001 gttatgtgct ctgtcatctg ttcgaccagc ccacgcatac gtcagccatg tttatttgcg
    15061 atcgctgcgg cgcggtgaaa gaagagtgtg cagaaggcgt ggaagacatt atgcatacgc
    15121 tggcggcaaa aatggggttt gccctgcggc ataatgtgat tgaagcacat gggctctgtg
    15181 cggcatgcgt ggaagtggaa gcgtgtcgtc atcctgaaca gtgccagcat gatcactctg
    15241 tgcaggtgaa aaagaaaccg cgttaagagg gcgtacatcc ttgtacacgt cgggcaggag
    15301 ggattaatta ccagcgatat tcattgcggg tttcccaatc cacgacctct ttttctgcct
    15361 gatctttctg ataaccataa cgttcctgga ttttaccgac cagttgatca cgtttacctt
    15421 caatgatcgt catatcatca tcggtcagtt tgccccattg ctctttcact ttacctttaa
    15481 actgtttcca gttaccgccg gcttcatctt tattcataat caagacctca tcgttaggtt
    15541 gtgaatgaga gtacgttcac ttttcttctg aacgtgagat taagtgtagt cagcaatttg
    15601 gcttaggata atttattcag aatttttaac cgtcacgttg cggcaaacca ggtaccgttg
    15661 cgccagtgac gccgccagat agccgccaga gaaagcccgc gcaacgccag aaagacggtt
    15721 aatgccagcc acaaaccatg attacccagc cacggcagcg taaggagcgt cagcgcaaaa
    15781 cctgcggcgg ccaccgccat actgttacgc atttcggcgg cacgcgttgc gcctataaac
    15841 atgccgtcca gcagataaca ccagacgcca accaacggca aaatcacctg ccagataaga
    15901 tagcggtcag ccagctgctg aatctgggtt aacgacgtca gcaacgcaat gatgtgttcc
    15961 cctgccagca aataaaccac cgaaaacagt aacgctacaa tccccgactg gcggcacgct
    16021 gcccgccata catccagcaa ctggctaccg tcgcgcgcac cgtatgcctg accggagtgc
    16081 gcttcaaccg cgtaggcaaa accatcaagc gcataggcgg taaaggtgag tagcgtcatc
    16141 agaaccgcgt taacagcgat aatgtcactc cccagtcgcg caccaagtac ggtgatcgcg
    16201 ccgaaacaga gttgcaacaa cagcgaacgc agcatgatat cgcggttaag cgccagcaag
    16261 cgacggaagt ttcctcgcca ggcagttttc agcatttcgc cggaaattcc gcgtagtttg
    16321 aggattttac gcaccattag cagaccaatc agcaatgttg catattccgc aataaccgtc
    16381 gccagcgccg cgccctgcac gttcatatgc agccccatca ccagccagac atccagcaca
    16441 atgttgagga tattgccgac cactaacaaa attactggcg cacgggcata ttgcacgccg
    16501 agtaaccaac cgagtaatac cagattcgcc agcgacgccg gagcgcttaa ccagcggatt
    16561 tcaagaaagc gccgcgcctg ttccaggacc gcttcactac cgccaacaat atgcagcgcc
    16621 agatcgataa tcggcgtacg caacagcgca attaacgccc cggcccccaa cgccaacagc
    16681 aacggttgca ccagcgcacg ggctaatgcc tgaggatttt tggcaccata agcctgcgca
    16741 gtcagcccgg tggtgctcat gcgtaaaaac agcaacagca taaagagaaa gctggtcgcc
    16801 gttgcaccaa ccgccacgcc gcccaaataa accggactat caagatgacc aattaccgcc
    16861 gtatcgacca gtcccagcaa cggaacggtg atattggaga aaatcatggg taaggcgaga
    16921 tgccagagtg ctttatcaga tgaagtgagg aatgccatgc agacaagcct gatgaagaga
    16981 gatgaaaaac aaaccgcgac gccaagcggc atcgcggtct cagagatatg ttacagccag
    17041 tcgccgttgc gaataacccc aaccgccagc ccttcaatgg tgaagctctg ctgacgaagg
    17101 tcaacgacaa ttggtttaaa ctcgctattt tctggcaaca gttcgacttt attgccctgt
    17161 tttttcaggc gcttaacggt aacttcgtca tcaatacgtg cgacaacgac ctgaccgtta
    17221 cgtacatcct gagttttatg cactgccagc aagtcgccat ccataatgcc gatatctttc
    17281 atcgacatcc cgctgacgcg cagcaggaaa tcagcattcg gcttgaacaa ggaaggatcg
    17341 acctgataat gaccttcaat atgctgttgc gccagaagcg gttcaccggc agccacacga
    17401 cctaccagcg gcaacccttc ttcctcttcc tgcaacagac gaatcccgcg tgatgcgccg
    17461 gaaacaattt caataacgcc tttgcgtgcc agcgccttca gatgttcttc agccgcgttt
    17521 ggggaacgga accccaaacg ctgcgcgatt tccgcacgcg tcggcggcat acctgtctgg
    17581 ctgatgtgat cacggatgag atcaaacacc tcttgttgcc tggccgttaa cgctttcatt
    17641 ccgccccctg ggtgtatata cagttatact gtgagtatat acagcaaaag gcgattttgg
    17701 aaccataaac tgcacaataa accagagatt tatcgaaatc tggaagggtt atccaaaatg
    17761 cgaccataac aggatgcacc aggtaatcac ggcgacgata atggcaatca gcaccgcggc
    17821 ggatcccata tcttttgcgc gtccggaaag ctcatggtat tcagagccaa ttcggtcaac
    17881 cactgcttcg atggcgctat tgaggatttc cacaatcatc accagcatca cggagctgat
    17941 aagcagcacg cgggtaattg cgtccacatc cagccagcag gcgatgacca ccgccaacaa
    18001 taccgctacg ccttcctgac ggaatgccgc ttcgttgatc catgcagcgc gtaaaccttt
    18061 ccaggaatag ccagcagctt tgataattcg ggtgaatcca gttgtattat tggccattga
    18121 aagaaccttt ttacattatg agcgtcaata tcagtgtacc gttaaatccg atgattcggg
    18181 tttgcgtaac gccgcgcgaa acatgagcgg ataccacaga atttcccatg actttctgct
    18241 atccttgccg cgcatttgca ttattaacca gaggctttac atcgtttatg tccggctggc
    18301 cacgaattta ctacaaatta ctgaatttac cattaagcat cctggtaaaa agcaagtcta
    18361 ttccggcaga tcctgccccg gaactggggc tggatacctc tcgtccaatt atgtacgttt
    18421 taccgtacaa ctcgaaagca gatttgctga cgttgcgcgc ccagtgtctg gcacatgatt
    18481 tgcctgaccc gttagagccg ctggaaatcg acggcacgct actgccgcgc tatgtgttca
    18541 ttcacggcgg gccgcgtgta ttcacctatt acacgccgaa agaagagtct attaagcttt
    18601 tccacgacta tctcgatttg caccgtagca acccaaatct ggatgtgcag atggtgccag
    18661 tatcggtgat gtttggtcgc gcgccggggc gtgaaaaagg cgaagtgaac ccgccgctgc
    18721 gtatgcttaa cggcgtacag aaatttttcg ctgtactgtg gctcggtcgc gacagttttg
    18781 tgcgtttctc gccgtcagtg tcgctgcgcc gtatggcgga tgaacacggc acggataaaa
    18841 ctatcgctca gaaactggcg cgcgtggcgc gtatgcactt tgcccgtcaa cgtctggctg
    18901 ccgtaggccc acgtctgcct gctcgtcagg atctgtttaa taagctgctc gcctcccgcg
    18961 ccattgccaa agcggtagaa gatgaagcgc gcagcaaaaa aatctcccat gaaaaagcgc
    19021 agcagaacgc gattgcgctg atggaagaga ttgcggcgaa tttctcttac gagatgatcc
    19081 gcctgactga ccgcattctg ggcttcacct ggaaccgatt ttatcagggc atcaacgtcc
    19141 ataacgccga gcgcgttcgc cagctggccc acgacggtca tgagctggtg tatgtgcctt
    19201 gccaccgcag tcacatggac tacctgctac tttcttacgt gctgtaccac caggggctgg
    19261 tgccgccgca tatcgccgcc gggatcaacc tgaacttctg gccggccggg ccgattttcc
    19321 gccgtctggg ggcgttcttt attcgccgta cttttaaagg caataaactt tattccaccg
    19381 ttttccgtga gtatctcggc gaactgttca gccgtggtta ttccgtcgag tacttcgtgg
    19441 aaggcggtcg ttcccgcacg gggcgtttgc tggatccgaa aaccggtacg ctgtcgatga
    19501 ccattcaggc gatgctgcgt ggcggcacgc gtccgattac gctgattccg atctatatcg
    19561 gttatgagca cgtcatggaa gtgggtactt acgccaaaga actgcgcggc gcgacgaaag
    19621 agaaagagag cctgccgcag atgctgcgcg gtttaagcaa gctgcgtaat ctcggtcagg
    19681 gttacgtcaa cttcggtgaa ccaatgccgt tgatgaccta ccttaaccag cacgtaccag
    19741 actggcgtga atctatcgat cccatcgaag cggtgcgtcc ggcctggtta acgccgacgg
    19801 tcaataatat tgctgccgat ctgatggtac gcattaacaa cgcaggcgcg gcaaacgcca
    19861 tgaacctgtg ctgtactgcg ctactggcat cacgtcagcg ctcactcacc cgcgagcagt
    19921 taaccgagca actcaactgc tacctggatc tgatgcgcaa cgtaccttac tccacggact
    19981 ctaccgttcc ttcagccagc gccagcgagc ttatcgatca cgcgctgcaa atgaacaagt
    20041 ttgaagtcga gaaagacact atcggcgaca tcatcattct gccacgcgag caagcggtgc
    20101 tgatgaccta ctatcgcaac aacattgcgc atatgttggt gctgccttcg ctgatggcgg
    20161 caattgtcac ccagcatcgc cacatctccc gcgacgtatt gatggagcac gtcaatgtgc
    20221 tttacccaat gctgaaagcg gagctgttcc tgcgctggga tcgcgacgag ttgccggacg
    20281 ttattgatgc gctggcaaat gagatgcaac gtcaggggct gattaccctg caagatgatg
    20341 agttgcatat caacccggcg cattctcgca ccctacagct gctggccgca ggcgcgcgcg
    20401 aaacgctgca acgttatgcc atcaccttct ggttgttgag tgccaacccg tcgatcaacc
    20461 gcggtacgct ggagaaagag agccgcaccg ttgcgcaacg tctctccgtg ctgcacggca
    20521 tcaacgcgcc ggagttcttc gacaaggcgg tgttcagttc tctggtgctg acgctgcgtg
    20581 atgaagggta tatcagcgat agcggcgatg ccgaaccagc agaaacgatg aaggtttatc
    20641 agttgctggc ggagttgatt acatcagacg tgcgtttgac gattgagagt gcgacgcagg
    20701 gcgaagggta atcagagaga attgccggat gcggcgaaaa cgccttatcc ggcctaccat
    20761 gacctgcaaa ttcaataaat tgcgattcac cgggtaggcc tgagaagcgc aacgcatcag
    20821 gcaacccaga agaaagccgg atgatcatcc ggctttttta catcatcaga aatgccagta
    20881 actcattgcc agtcctaaaa acagtaccag accaacatag ttattattca taaatgcttt
    20941 aaagcaggct tcacgctcgc ggttggcaat cagtttttgt tgataaacaa acagcgcgcc
    21001 agccaccaca attgaccagt aatatcccca gcctaagcca tttaactcac cgatgatcgc
    21061 catcagtgcc agtacgccaa tctgcaaaat accaataatc aatttatcgt attggccgaa
    21121 caggattgcc gtggatttaa tgccaatctt cacatcatca tcgcggtcaa ccatcgcata
    21181 ctgcgtgtcg taagccaccg cccagagaat attggcgagg aacattaacc agcaactcaa
    21241 tggcaccgac tcactcacag cggcaaaagc cattggaatc gaccagccaa acgccgcgcc
    21301 cagcaccact tgcgggagat gggtataccg cttcataaac gggtacaccc acgccaatgc
    21361 cagagcggca atcgacaaca gaatggtcat cgtattcagc gtcagaacca gtaaaaacga
    21421 aatcagtacc agcactacaa acagcgcgcg cgcctctttc tctgttaccg cgccgctggg
    21481 aagtggtcgg ttcgccgtgc gcttaacatg accatcaaac ttgcgatcag cataatcatt
    21541 caccacgcaa ccagcagcgc gcatcagcca gacacccgcg acaaacaccg ccatgatcca
    21601 gagctgagga acgcccggtg tcgccaccca caacgcccat aatgttggcc agagcagcag
    21661 taacgcgcca attggcttat ccgtacgcat taagcgatga aacgccagca gcttattctg
    21721 cgtcagactc cactccatat ttttttcctc ttagtacaac ggtgacgccg gtaaaaacag
    21781 ttctgttagc aacagcggtt taccgcttaa tcgcaggcgg gaacgtcgcc cccacagtcc
    21841 ggcatcacgg cctatctcga taaaatcccg ggttagtgtc gatgatgtga acagatagcg
    21901 tcctaacggc gttttaccca atttttgtaa cgctagttcc ggcccgctta atgttgatac
    21961 aggaacgacg gtacgaccgg caagccacgg ttcaccatcg gcacataaca aaatttcacg
    22021 taaccagtaa cgagactctt tcggcagcag cggcagttct tcggggattt cattctgctc
    22081 gacaaacccc tcgcggatca tcgtcacact caccgttttt ccctgctgtt caaaacgttt
    22141 tgtcatggaa tcctccagca acagccagtc gagcagttgc ggatccaggg cagggatctc
    22201 tttaaaatag cgcagcgcac gcagttgcgt taacgcgggg tgtgacatgc cgaactctcc
    22261 gttacataac gtaaaggcat tgtatcgcag aaaagtgttt acgggagatg taaagcaaat
    22321 taagtgatct aatgcgcaac aaaaacgcaa caatcgcatg aagtggtaaa acgctgttga
    22381 accttacgct ttgccgatgc ccgtgctggc gagggcaacc ataggggcat caccgcccat
    22441 gatgctagga acggtgatgt tttcctttac ttaaatacct ttattcttta tacgagtagc
    22501 tcgccaactc gcagtaaaaa ttgggaaaag cgcatattta ccatgagatt tggagctgaa
    22561 aagtgtttcc tgagtaagat taataggcaa catcttatgt ctggatttat agatacacgt
    22621 ggaattccaa ttggtatctt ttgtaggatt caacatatca tgtaagcgct gggagcttac
    22681 caccataaca ggcccatcat cgacagagga gaacaggata aatatattat cgtttcgtgt
    22741 taatttatta gtgttaaagg ttcttaactt ttcttcagtt tctataaata taggtgaaac
    22801 atcatcacta cgaatccact ctttataaag ataccaggca tttgctttga tcatatcatc
    22861 cgttgatcgt aaggcctgat cgataagttg gataaaaaaa ttgttatttt tatcaaccag
    22921 ccctttattc atattaaatc tgttaataaa atggatcatc tgttgggtat cgaaattcac
    22981 cttactgaat cgatgttgac gcataaaatt atcgaacttg tcatcttccc cagaatgtat
    23041 ataactacgt ataattggat cttttatata aggacaatgg ctaaatgttt tcttcatatt
    23101 cttaataaag tttttattaa caatattgag aatttttaat tgcatcacca gttttactgc
    23161 cagaacggat ttcaatatca ttgcattatc tggcaatgta tcaatcattt ttataatgtt
    23221 attgatataa ccatcgagat cttcattccc aaatatatct gaataatcaa tagttaattc
    23281 caattgttct gtaatgcaat cttaaaaatt ccctgtcgac attcgcacat gatcaaacat
    23341 aacagctgtc aaattcgatt taataaatga tatttctttg gcagacagaa actctgctct
    23401 ggtgaagtgc gaagcgtaaa gataacaatt atcgaagttt gaatttctaa tctgacattc
    23461 gcgaaaatta ctatgaccaa gttgactctg cgagaagtca actgtatcaa gaatattttg
    23521 gctaaaatca acacattcgc cactcgtttt gagaaaaatc acattgtgta aacgtcgaca
    23581 tttaaatatg acattatcaa ctgttgcctt agtaaaattg caattctcaa tttctttaca
    23641 ttcgaaagtt acatgttgta gatttgatga taaaaaagac accttagaaa taacgtcacc
    23701 tttaaaatga caatttgtta aatcaaaatc agacaggtcg atatacccct gcgcatcttt
    23761 atccatcaga aaattacaag cgcattctat tttttcattt ctgtagaatt ttacacttat
    23821 agtatcatta ctaattgaga cttcacgtaa catatttatt gcatgatttt tttcgttccg
    23881 ttgttttata tttaaaaata tatcactgat ataaataaag taatctctgt ttaaacctgt
    23941 caccccattg aaattacgta gtaaatcaag atctactata ttatttcctt gcaccacatc
    24001 atgtttcatc aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct
    24061 gtaatacaga aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa
    24121 aggaaaagaa tctctgataa tgcattgaaa taatggacat tcttagtgaa gaagaagtga
    24181 agtactataa aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag
    24241 acgcaccagt tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact
    24301 gctgataaag gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt
    24361 taacgcctta tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact
    24421 ttccgtgtcg ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac
    24481 agctggtgcc gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg
    24541 tcctaccaac acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct
    24601 ggtatgacga gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata
    24661 ggctttagcc gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac
    24721 cagaacataa agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg
    24781 ctctgcactc atcacgccta gttcctggta ggtgaaataa ctgctgggga agaaggctga
    24841 tggggtcatg ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt
    24901 cacttcgctg gtcagcgtca ggggcagttc gccaatgttt gccgggacgc tgtacgcagc
    24961 aaccggacca ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt
    25021 ctgggtttta gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat
    25081 ggctggcgcg tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg
    25141 cgcgcttgcc agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat
    25201 tgttttcacc tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat
    25261 aatcagaggg gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc
    25321 gtaaacgcct tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga
    25381 aggtccactc gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg
    25441 ctttgccgta gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat
    25501 cccacttggc gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag
    25561 cctgccattg ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg
    25621 attcgacgtt gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca
    25681 tcgggcgaat accaacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct
    25741 ggtacatacc cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga
    25801 ggatacgcag catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg
    25861 cgacgccaga accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact
    25921 gaacaacaaa cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc
    25981 agccgtcttt cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt
    26041 agtcgacacc cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat
    26101 cgaaaacgtc gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg
    26161 aagaaccacc agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat
    26221 cgatgttttc cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat
    26281 gaacgtcatg acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt
    26341 cgatcaggtt ttttccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt
    26401 cattctgttg cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct
    26461 ctttccacac ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac
    26521 ggtatttact ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc
    26581 agccaatacc ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta
    26641 cgcccgctgc gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc
    26701 ctgagtcatt gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg
    26761 tgcttaaacg cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag
    26821 atggcaacgc tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac
    26881 gtcgttctgg cggtacacca ggttttgacg aatggaaggg atctggatat agatttgggt
    26941 ttcgttgccg agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc
    27001 actcggcagt agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc
    27061 acggctttca acaggcagcc agacttgttg acgatttggc atcggcagct ccacctgcac
    27121 ttgatcgatt gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat
    27181 aaatccggcg acaaaacggt ctgccggata gtggtacagt tccagcggtt tcccaacctg
    27241 tgccacgcga ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg
    27301 atcgtgggtg acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat
    27361 acgcatttgc acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac
    27421 gcttggctcg gccaccagcg tacggccaat cgccacgcgc tgacgctgac caccggagag
    27481 cgctttcggt ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac
    27541 gcgttggtta atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc
    27601 tgctactgac aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc
    27661 tgctggcgga gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt
    27721 ctcaagcccg gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa
    27781 caccacgaat tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc
    27841 ccaggctttc gttacatttt gcagctgtac gctcgccatg cccttctctc tttgtaacaa
    27901 cctgtcatcg acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa
    27961 atcctccacc ccctggcttt tttatggggg aggaggcggg aggatgagaa cgcggcttct
    28021 gtgaactaaa ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga
    28081 ttttatgtgc gcatccccac attaccgcca attctgtaac agagatcaca caaagcaacg
    28141 gtggggcgta ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt
    28201 ttaggtgttt tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg
    28261 tgcacgcatc ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc
    28321 caaaatcgaa gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct
    28381 cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc
    28441 ggataaactg gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat
    28501 cttctgggcg cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac
    28561 cccggacaaa gcgttccagg acaagctgta tccgtttaca tgggatgccg tacgttacaa
    28621 cggcaagctg attgcttacc caatcgctgt tgaagcgtta tcgctgattt ataacaaaga
    28681 cctgctgccg aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa
    28741 agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct
    28801 gattgctgct gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga
    28861 cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa
    28921 aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg
    28981 cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt
    29041 gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg
    29101 cgtgctgagc accggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct
    29161 cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg
    29221 tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac
    29281 catggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg
    29341 gtatgccgta cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc
    29401 cctgaaagac gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg
    29461 aacgccttgt ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt
    29521 cgcatcaggc agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa
    29581 gaaccccatg gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc
    29641 agtgctaggt ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg
    29701 ggaatacctg ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc
    29761 caatcgtaaa gcctacgtct ggcgctatgt ttacccggga atggctggaa tgggattatt
    29821 cgtcctcttc cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa
    29881 ccagctgact tttgaacgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa
    29941 aacctataac tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagcgacgg
    30001 cgaaaccggc aaaaattacc tctccgacgc gtttaaattt ggcggcgagc aaaaactgca
    30061 actgaaagaa acgaccgccc agcccgaagg cgaacgcgcg aatctgcgcg tgattaccca
    30121 gaatcgtcag gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat
    30181 gagctccctg cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac
    30241 gttgacgaat aatcagagtg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca
    30301 gtccattacc gccgacggca actgggggga tgaaaagcta agccccggtt acaccgtgac
    30361 caccggctgg aaaaacttta cccgcgtctt taccgacgaa ggcattcaga aaccgttcct
    30421 cgccattttc gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt
    30481 cggcatggtt ctggcatgtc tggtgcagtg ggaagcgttg cgcggcaaag cggtctatcg
    30541 cgtcctgctg attctgccct acgcggtgcc atcgttcatt tcaatcttga ttttcaaagg
    30601 attgtttaac cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa
    30661 gcccgcctgg ttcagcgatc cgaccaccgc tcgcacgatg ctgattatcg tcaatacctg
    30721 gctgggttat ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga
    30781 tttgtatgaa gcctcagcaa tggatggcgc aggtccgttc cagaacttct ttaagattac
    30841 gctgccgctg ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt
    30901 taacaacttc gtgctgattc agctgttaac caacggcggc ccggatcgtc ttggcacgac
    30961 cacgccagcc ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg
    31021 cggcgggggt caggacttcg gtctggcggc agcaattgcc acgctgatct tcctgctggt
    31081 gggtgcgctg gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata
    31141 acaaaaatgg caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg
    31201 ctactgctac tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctcg
    31261 ctgcgtcagg gaaacttcgc gaccggtagc ctgatcccgg agcaaatctc ctgggatcac
    31321 tggaaactgg cgttaggttt tagcgttgaa caggctgatg gtcgcattac gccaccgcca
    31381 ttcccggtac tgctgtggct gtggaactcg gtaaaggtcg ctgggatttc cgcgattggc
    31441 attgtggcgc tctccaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa
    31501 gcgacgctgc tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc
    31561 gcgttgtatg cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact
    31621 cacggcggcg taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa
    31681 ggctatttcg aaaccatcga cagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca
    31741 ccgtggcagg ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta
    31801 ttcatcctgt cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt
    31861 gacgtaaaca gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac
    31921 ctgtggggtg actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc
    31981 ttgctggctc aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga
    32041 tgttgttctg ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact
    32101 attattcggc gctccacgga gcgcctt
//
LOCUS       DBKELA010000038        32122 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.038, whole genome shotgun sequence.
ACCESSION   DBKELA010000038 DBKELA010000000
VERSION     DBKELA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.038"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..945
                     /gene="espL1"
                     /locus_tag="ACUQR0_001768"
                     /pseudo
     CDS             1..945
                     /gene="espL1"
                     /locus_tag="ACUQR0_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1117..1224
                     /gene="yniD"
                     /locus_tag="ACUQR0_001769"
     CDS             1117..1224
                     /gene="yniD"
                     /locus_tag="ACUQR0_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HHV9108853.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1277..2035)
                     /locus_tag="ACUQR0_001770"
     CDS             complement(1277..2035)
                     /locus_tag="ACUQR0_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HHV9108854.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2322..3251
                     /gene="pfkB"
                     /locus_tag="ACUQR0_001771"
     CDS             2322..3251
                     /gene="pfkB"
                     /locus_tag="ACUQR0_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HHV9108855.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3352..3642
                     /gene="ghoS"
                     /locus_tag="ACUQR0_001772"
     CDS             3352..3642
                     /gene="ghoS"
                     /locus_tag="ACUQR0_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HHV9108856.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3748..4608
                     /locus_tag="ACUQR0_001773"
     CDS             3748..4608
                     /locus_tag="ACUQR0_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HHV9108857.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4649..5185)
                     /locus_tag="ACUQR0_001774"
     CDS             complement(4649..5185)
                     /locus_tag="ACUQR0_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HHV9108858.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5332..6000
                     /gene="hxpB"
                     /locus_tag="ACUQR0_001775"
     CDS             5332..6000
                     /gene="hxpB"
                     /locus_tag="ACUQR0_001775"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HHV9108859.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLCDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6163..6753
                     /locus_tag="ACUQR0_001776"
     CDS             6163..6753
                     /locus_tag="ACUQR0_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9108860.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6886..8276
                     /gene="tcyP"
                     /locus_tag="ACUQR0_001777"
                     /pseudo
     CDS             6886..8276
                     /gene="tcyP"
                     /locus_tag="ACUQR0_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8280..9084)
                     /locus_tag="ACUQR0_001778"
                     /pseudo
     CDS             complement(8280..9084)
                     /locus_tag="ACUQR0_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(9373..9636)
                     /gene="cedA"
                     /locus_tag="ACUQR0_001779"
     CDS             complement(9373..9636)
                     /gene="cedA"
                     /locus_tag="ACUQR0_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HHV9108861.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9819..12080
                     /gene="katE"
                     /locus_tag="ACUQR0_001780"
     CDS             9819..12080
                     /gene="katE"
                     /locus_tag="ACUQR0_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HHV9108862.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12127..12885)
                     /gene="chbG"
                     /locus_tag="ACUQR0_001781"
     CDS             complement(12127..12885)
                     /gene="chbG"
                     /locus_tag="ACUQR0_001781"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HHV9108863.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12898..14250)
                     /gene="celF"
                     /locus_tag="ACUQR0_001782"
                     /gene_synonym="chbF"
     CDS             complement(12898..14250)
                     /gene="celF"
                     /locus_tag="ACUQR0_001782"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HHV9108864.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14355..15197)
                     /gene="chbR"
                     /locus_tag="ACUQR0_001783"
                     /pseudo
     CDS             complement(14355..15197)
                     /gene="chbR"
                     /locus_tag="ACUQR0_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15205..15555)
                     /gene="chbA"
                     /locus_tag="ACUQR0_001784"
     CDS             complement(15205..15555)
                     /gene="chbA"
                     /locus_tag="ACUQR0_001784"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HHV9108865.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15614..16963)
                     /gene="chbC"
                     /locus_tag="ACUQR0_001785"
                     /pseudo
     CDS             complement(15614..16963)
                     /gene="chbC"
                     /locus_tag="ACUQR0_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(17048..17368)
                     /gene="chbB"
                     /locus_tag="ACUQR0_001786"
     CDS             complement(17048..17368)
                     /gene="chbB"
                     /locus_tag="ACUQR0_001786"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HHV9108866.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17667..18005)
                     /gene="osmE"
                     /locus_tag="ACUQR0_001787"
     CDS             complement(17667..18005)
                     /gene="osmE"
                     /locus_tag="ACUQR0_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HHV9108867.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18207..19034
                     /gene="nadE"
                     /locus_tag="ACUQR0_001788"
     CDS             18207..19034
                     /gene="nadE"
                     /locus_tag="ACUQR0_001788"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HHV9108868.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19264..20151
                     /gene="cho"
                     /locus_tag="ACUQR0_001789"
     CDS             19264..20151
                     /gene="cho"
                     /locus_tag="ACUQR0_001789"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HHV9108869.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20111..20686)
                     /gene="ves"
                     /locus_tag="ACUQR0_001790"
     CDS             complement(20111..20686)
                     /gene="ves"
                     /locus_tag="ACUQR0_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HHV9108870.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20889..21374)
                     /gene="spy"
                     /locus_tag="ACUQR0_001791"
     CDS             complement(20889..21374)
                     /gene="spy"
                     /locus_tag="ACUQR0_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HHV9108871.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21704..22672)
                     /gene="astE"
                     /locus_tag="ACUQR0_001792"
     CDS             complement(21704..22672)
                     /gene="astE"
                     /locus_tag="ACUQR0_001792"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HHV9108872.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22665..23998)
                     /gene="astB"
                     /locus_tag="ACUQR0_001793"
                     /pseudo
     CDS             complement(22665..23998)
                     /gene="astB"
                     /locus_tag="ACUQR0_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(23995..25473)
                     /gene="astD"
                     /locus_tag="ACUQR0_001794"
     CDS             complement(23995..25473)
                     /gene="astD"
                     /locus_tag="ACUQR0_001794"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HHV9108873.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25470..26504)
                     /gene="astA"
                     /locus_tag="ACUQR0_001795"
     CDS             complement(25470..26504)
                     /gene="astA"
                     /locus_tag="ACUQR0_001795"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HHV9108874.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26501..27721)
                     /gene="astC"
                     /locus_tag="ACUQR0_001796"
                     /pseudo
     CDS             complement(26501..27721)
                     /gene="astC"
                     /locus_tag="ACUQR0_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28167..28973
                     /gene="xthA"
                     /locus_tag="ACUQR0_001797"
     CDS             28167..28973
                     /gene="xthA"
                     /locus_tag="ACUQR0_001797"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HHV9108875.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29140..29848
                     /locus_tag="ACUQR0_001798"
                     /pseudo
     CDS             29140..29848
                     /locus_tag="ACUQR0_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29853..30530
                     /gene="ydjY"
                     /locus_tag="ACUQR0_001799"
     CDS             29853..30530
                     /gene="ydjY"
                     /locus_tag="ACUQR0_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HHV9108876.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30545..31252
                     /locus_tag="ACUQR0_001800"
     CDS             30545..31252
                     /locus_tag="ACUQR0_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HHV9108877.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31252..31800
                     /locus_tag="ACUQR0_001801"
     CDS             31252..31800
                     /locus_tag="ACUQR0_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HHV9108878.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31810..>32122
                     /locus_tag="ACUQR0_001802"
     CDS             31810..>32122
                     /locus_tag="ACUQR0_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHV9108879.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NF"
BASE COUNT         8240 a         8234 c         7609 g         8039 t
ORIGIN      
        1 gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta cctgtgggag
       61 ttccaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct atacatccat
      121 cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca ttattcaaac
      181 agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag aatagcgttg
      241 gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg aaaatattta
      301 tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat ctggttaaat
      361 tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg ttgtatggat
      421 tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca ctaagagctt
      481 ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg tgagccagga
      541 ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct ctctaaaatt
      601 aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt attaaaagga
      661 gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa tgaagacgtc
      721 gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt tagtcaacat
      781 cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt tcatatagcc
      841 attcatcata agcattataa aactgtagaa acatattatg ctgctataaa tgtcatcagc
      901 caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc tatttacgat
      961 atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac catgtgcaca
     1021 acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt ttgttacact
     1081 ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa
     1141 aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt
     1201 tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac cgcataacac
     1261 ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga cggtcggtat
     1321 gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc accttcagtc
     1381 caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt gtgtcttccg
     1441 cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag
     1501 ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat ttgtcgtaac
     1561 gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa aactgacgac
     1621 cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc
     1681 ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc ccgcccgccg
     1741 catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg
     1801 accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt
     1861 tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag
     1921 cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg
     1981 caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc
     2041 aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa
     2101 agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact tagcaatgta
     2161 ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt atatatattt
     2221 atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt cactttccgc
     2281 tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt
     2341 tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa
     2401 aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg
     2461 ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg
     2521 aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag
     2581 actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag tatcgttttg
     2641 ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg
     2701 aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt gtgaagctgg
     2761 aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc atcatcgaca
     2821 gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg gttaagccta
     2881 accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac gatgtccgca
     2941 aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc
     3001 cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca ccaccggtga
     3061 aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg
     3121 cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca
     3181 cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc
     3241 tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac ctgtcggcaa
     3301 cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac tatggccagt
     3361 ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt
     3421 aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag
     3481 ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca aagtgaagaa
     3541 gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatc
     3601 accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa
     3661 tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct aaccttatga
     3721 tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag
     3781 cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc
     3841 gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga aagggaactg
     3901 cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc
     3961 accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat
     4021 tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg
     4081 cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa ctcgctctcc
     4141 acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc tgaacaacgg
     4201 attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc
     4261 atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc tctgttgcac
     4321 ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct
     4381 gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca tactgaacag
     4441 ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa
     4501 cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt tggcggtcag
     4561 catttggtta ttgctcagca gtcattggat agattattag cagcatgata tgggttgagg
     4621 ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat
     4681 aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc aggaaaatag
     4741 tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt
     4801 tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac
     4861 tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc
     4921 agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac cagaacagat
     4981 tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag tcgagcatga
     5041 ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca
     5101 atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa
     5161 tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta
     5221 gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat
     5281 cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc
     5341 ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct
     5401 ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat ctcccgtcgt
     5461 aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc
     5521 cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc
     5581 atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc tgttgcgtta
     5641 tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa
     5701 aaagtgttga ccatgtttga cttatgcgac agtttcgatg ccctcgcctc ggctgaaaaa
     5761 ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt
     5821 gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc ttctaaagca
     5881 gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta
     5941 ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa
     6001 tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca ttcaactgac
     6061 ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa
     6121 aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac
     6181 cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg
     6241 gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac
     6301 atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa accgatcgcc
     6361 cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca
     6421 accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct acaaggaatg
     6481 gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg tgttcccctg
     6541 ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa gggcaaccaa
     6601 ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc ccattcatta
     6661 cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca gatccagttt
     6721 catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct ttctggaata
     6781 agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta
     6841 tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta
     6901 attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccataaa
     6961 cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt
     7021 gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt
     7081 aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc
     7141 gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt
     7201 tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt cggtgtgctg
     7261 gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca
     7321 cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag cgttccgcag
     7381 ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg
     7441 tcaatcatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg
     7501 aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct acaaagctgg
     7561 gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg
     7621 accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc
     7681 gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct gggcattaat
     7741 ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc
     7801 agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc
     7861 gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc
     7921 tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt ggcattaacc
     7981 cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt tccgcaggcg
     8041 ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct gcgatgggcc
     8101 tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac atgggccgta
     8161 cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag tggctgaagc
     8221 aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac cgttaattct
     8281 tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa atgttccatc
     8341 gcggcgatgc atactgactc atatttaaac agatcgtcag cgaaaaaaat gtccaactta
     8401 tcaatacatt cctggctcaa tgaatgtgcc tgtagtgaga agcttaaaac ctcatacccg
     8461 agacgttttc taagtgaact atctggaata ctcataatat cttcaagcac accacttccc
     8521 attcccggaa gatttagtgg attaaaaaga agattgatat taagcattga tattatcgaa
     8581 ggaaacttta ggagactctc attttttttg aatcgcagca aaaatgctcc cggcaggatg
     8641 tatataattt ttttcatagt atatagtcct taaataatga ataaccaacc tattttttat
     8701 attgatcaaa ttcgtcattt tttcgggtcg gaattcacag gtaaaatcca tcccaacaaa
     8761 attctcatta atatctctta tccgaaactc actgccataa agattattaa ttatcactct
     8821 atatttaaca ttgtcctgca gatacgtatc aaaactaagt gctggcgctc ctagctttga
     8881 ggcatcatag ttaatgttag acatataggc aatcatacga ttgaagttcg actctgctat
     8941 ttcatcgcag tcacattgac ccgttgatat aaagaaagtg agtgccgcca tggtagactc
     9001 aagcctttct ttaaaagagt cgaaagccag gcttttaata tttaaatcac cataattact
     9061 ctgtattaag tttgtagaaa acatctcccg cctcatattg ttaacaaaat tattatctta
     9121 tttaaatcta agtcatttac aatataagtt taagagcgac gccacaggat gaactatcaa
     9181 aaataactca tcatgattag caaaacttaa ccattttaaa ataaataaat aattaaagaa
     9241 aaaagatcac ttatttatag caatagctcg tcaaaggcag ctttttgtta caggtggttt
     9301 gaatgaatgt agcaacgaaa tgcagaattt caggtcctgt aactcccggc aaaaccggga
     9361 ggtatgtaat ccttactcag tcacttcccc ttcctggcgg atctgatttg cccaacgttg
     9421 ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca tcagtacaaa
     9481 cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct ttatcgcatg
     9541 ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt tttgctggcg
     9601 gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa aaactgtagt
     9661 ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac cgtttccaga
     9721 atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt tgaccaaaac
     9781 agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacat aacgaaaaga
     9841 acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg gggatggact
     9901 cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg ccaggtgcac
     9961 aacctaccgc ccctgggagc ctgaaagccc ctgatacacg taacgaaaaa cttaattctc
    10021 tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca
    10081 tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg ctggaagatt
    10141 ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa cgtattgttc
    10201 atgcacgcgg ctcagcagct cacggttatt tccagccata taaaagctta agcgatatca
    10261 ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt aggttctcta
    10321 ccgttcaggg tggagctggc tctgccgata ccgtgcgtga tatccgtggt tttgccacca
    10381 agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca atcttcttta
    10441 tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa ccgcactggg
    10501 caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct ctgcaacctg
    10561 aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc agttaccgca
    10621 ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg aaggcaacgt
    10681 ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg gatgaagcac
    10741 aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa gccattgaag
    10801 caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa gatgaattca
    10861 agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg gtgcccgttc
    10921 agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc gaaaacgaac
    10981 aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac gatcccctgt
    11041 tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt gggccgaatt
    11101 tccatgagat tccgattaac cgcccgacct gcccttacca taatttccag cgtgacggca
    11161 tgcatcgtat ggggatcgac actaacccgg cgaattatga accgaactcg atcaacgata
    11221 actggccgcg cgaaacacca ccggggccga aacgcggcgg ttttgaatca taccaagagc
    11281 gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt tggcgaatat tattcccatc
    11341 cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc gatggtttca
    11401 gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg
    11461 cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc gaactgactg
    11521 acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag gatccatcct
    11581 taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg attttactta
    11641 atgatgaagt gagatcggca gaccttctgg ccattctcaa ggccatgaag gccaaaggcg
    11701 ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt
    11761 tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg gtcattgtcc
    11821 cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactat ctgatggaag
    11881 cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt aaagcaacaa
    11941 tcaaggtagc tgaccagggt gaagaaggga ttgtggaagc tgacagcacc gacggtagtt
    12001 ttatggatga actgttaacg ctgatggcag aacaccgcgt gtggtcacgc attcctaaga
    12061 ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc cctggggagt gaccttacca
    12121 ccttacttac acatcaagat aactacccaa ccgataacca cgctcagcaa ttgcatattt
    12181 caacgacgct gatgtcagca cttccagctc cgttaaccgt gggaaacagt aagcactctg
    12241 acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaag gatcaccccg
    12301 atggcttgcg tcatccagca cttgcaggaa cagtgcctcg ctaatctctt caccataaaa
    12361 cgcgctactg aatccctgcg aactgcgcag attaaccggt aaatcaccag cgttagatag
    12421 cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg caacaatcgg
    12481 gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag gtttgcgtcc
    12541 aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt cttccagcgg
    12601 taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat cgcgggttaa
    12661 ccccggcata gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc ctatggccag
    12721 actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat tcaccagcgc
    12781 cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt tctgaccttt
    12841 gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca gcctcggtta
    12901 atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt tctcgtgcgc
    12961 cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca acggactaag
    13021 gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc tggcagcaat
    13081 ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat gcgtaatgcg
    13141 cggatgtggc gtcgcgccat cgtgccccag cttacaggtc atttctaccg cccagtctgc
    13201 cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag cttgcttgtc
    13261 gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc caccgcgctg
    13321 ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa aaagttgttt
    13381 ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc ccatttcaat
    13441 agccagcatc tctttttgct tgaagtaata aagcaaataa gaacacggca acagattcag
    13501 agaacgaatt aagccttcgc taaatggcag atcgaaaata tttttaacgc cagatacttt
    13561 taactgcccg gatgccacac catcaagcaa ttcggcaaag cgcgacttgc catttaccag
    13621 cacatcctta atgaacacca tatggttgag gccaaacaaa tcgatagata aatcatcgct
    13681 gtctttcagc atcagaacat cgtgaataaa catcttcatg ccgatcggaa tattacacac
    13741 gccgataaaa cgtttaaatc cggtatgacg ataaacggct tcagtaacca ttcccgccgg
    13801 gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt ttacgatgtc
    13861 aaaaatcacc ggaatggtac gcagaccttt aaacaaaccg cccgcgccgt tggtttcctg
    13921 accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg gtaattggcc
    13981 aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc aatccagcgt
    14041 tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga gatcgaaaat
    14101 aatatccagt ttagctttgc caccctcgac atccaccagc cataattcgc tgaccggcaa
    14161 ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc tcccgccacc
    14221 aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag aatactgata
    14281 tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat atgtgaattg
    14341 tcaggtataa cgacttactg attaaattca gtcaatttct tacgatatct cttaggcgta
    14401 aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata accggcctca
    14461 aacgcaatat ccgtcacgga atagttggtc atttccagtt gtttttttgc aaaattaata
    14521 cggatctcat taataatctg cattggtgtt ttgccataat atcgttgagt cgctcgcgtc
    14581 aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga ttcactaaac
    14641 tgctctttat catgcatctt ttctaccgta cttttcagcc actacggtac atcatcaatc
    14701 acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac catctcaaca
    14761 aactcttcca gccctgtttc gcggaaattt aatgaagaaa tcactgtttc cacataggtg
    14821 agaaaagcgt tattggtccg gtatacctgc gaagcaacaa agcaataagg caacaacggc
    14881 aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatgcg cgtggcacca
    14941 aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc gccccgttcc
    15001 agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac tagagtaaat
    15061 tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttata gataaacaca
    15121 tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc cggcgcgtta
    15181 atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct caattaattc
    15241 agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt ggaccagcac
    15301 cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg tctgtaccag
    15361 atgcgcttca ttcaatgcca tccgtgactg atccatcatg gctttcgctg cggcaaaatc
    15421 gccctgcttc gcctgtttca atgcggcata cgccaggcta cgcgcttgtc cagagttgat
    15481 gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat caggaatatt
    15541 atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac ccgtaccggc
    15601 atcgattaaa atttcagggg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat
    15661 tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg atgccaaggt
    15721 tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac
    15781 cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg
    15841 ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg
    15901 ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg
    15961 gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca
    16021 ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct
    16081 tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct
    16141 gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca
    16201 gcgccagcgc gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc
    16261 agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat
    16321 ggaagttggt gccctaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa
    16381 taaagccggg aattaatgcc gagaaagaac gcgatactga agcaggtacg ctgtcgggca
    16441 gtttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa
    16501 taataatccc ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct
    16561 caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac ccagccgcca
    16621 gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt gccattaaag
    16681 acattattcc taatgttccg ttatatacgt tgccgccaat acctttcaga ccattaagtg
    16741 tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgac ccctccccaa
    16801 agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggtt aacggcatta
    16861 agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt ttaactgcaa
    16921 aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa tacccttaaa
    16981 aaccgcaatt taaatattac ggtattgatt tatgaaataa ctctttgacg ggaaaattta
    17041 aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta agcacgccta
    17101 aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg ttgggtaaca
    17161 aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg acatcggcat
    17221 tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc ggaacttcat
    17281 atttttctgc ctgtgcgcgc atttttgata ccagtaaaga gatagacatg cccgcagaac
    17341 aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt atctgtcagc
    17401 cagacactcc gcaagccttg acctgcttcc atgctctggg taacttgcga aaccaacatg
    17461 actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta tttctttgac
    17521 cttataaatt gtttctcctt gacctgtggt tatgaccctc ttcacatttc gggcaaataa
    17581 ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc ggtttccgtt
    17641 gttactcaag ctttcagacg aattgattac ttcgcagcct gtggatcagt gtcgtattca
    17701 gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc gacaaagtag
    17761 gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc gcgagcatgg
    17821 atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc ccggctcatg
    17881 ccttttttga cgtctttcac cacaggctgt acaaactggt ctttggtacg atcataagct
    17941 gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc catattcttg
    18001 ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac attgggcgct
    18061 ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg acgttttaac
    18121 caccattcag cccgctgtcg cttgtcgttt cagtagcaac gggttagctt taaggaagtt
    18181 ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata aaggcgctgg
    18241 gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtgtcgat tttctgaaaa
    18301 gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc ggcggtcagg
    18361 actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc caggaaactc
    18421 gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag gctgacgaac
    18481 aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc gttaatatca
    18541 agggcgcggt attggctagc gagcaggcat tgcgggaagc aggcattgaa ctgagcgatt
    18601 ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc attgcgggta
    18661 tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc ggattcttca
    18721 ctaaatatgg tgacggcggt acggatatta atccgctgta tcgtctcaac aaacgccagg
    18781 gtaaacagtt actgacggca ttaggttgcc cggaacacct ttataagaaa gccccaactg
    18841 ccgatcttga agatgatcgc ccttctctgc cagatgaagt ggcactcggc gtgacctatg
    18901 acaatatcga cgactatctg gaagggaaaa acctacctga acaggtcgcc agaacaatag
    18961 agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaacgtt ttcgatgatt
    19021 tctggaaaaa gtaataattt gcacctattg gattgtgcga aaaagagtaa tttgttcacg
    19081 ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca atacattgca
    19141 ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt gtaacctttg tcatcaaccc
    19201 gcctcttttt taatcgcctc ccgcctgtta cactggatag ataaccagca ttcggagtca
    19261 acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa
    19321 tatcccgaac atttacattc attccttaat gacttaccca cccgaccagg ggtgtatctg
    19381 tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcgttaa tatccgcagt
    19441 cgggtgcttt ctcatttacg caccccggat gaagccgcca tgctacggca atcccggcga
    19501 attagctgga tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc gcgattaatc
    19561 aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct ctgtgccctg
    19621 caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt ttcacgagcc
    19681 cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt gcagagcatc
    19741 gctgatgaac aaaaactttg ttatggcctg ttgggactgg aaccgttaag tcgcggtcgt
    19801 gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa agagagccat
    19861 gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgagtggt gtgttggcct
    19921 tggcaaggtg cggtggcact gaaagaacag cacccggaaa tgactcaata tcatattatt
    19981 caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac gttaattcgg
    20041 acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct gctttccggt
    20101 aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg atttcactga
    20161 acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg aaccagcacg
    20221 cgccctggtc ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta atcacaaaca
    20281 ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg actttcgcct
    20341 tacagacatc caggcgcgtc ataatattga aatccatcga catctgccct gccgtcagtt
    20401 tcgcttttac cacctggtcc gccgtaaagg caaaaggctg taacggtttt aaggtatggt
    20461 taaagcggtc tgcgctttca aggagcatct cgccgccttc cagcaacgtc actatccttt
    20521 ccatgccggg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg ctggcacgcc
    20581 agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca ccggcagcgt
    20641 ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc gctcccggcc
    20701 cgttcttttc attgttatcg ttcaggtaac cgtggatgat tatcggcaac tcgggtgatt
    20761 tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaag aaaaccgccg
    20821 atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg
    20881 ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga
    20941 cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaggat gttgtaaatt
    21001 ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc catttttgcg
    21061 atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc atgcattgcg
    21121 cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat gatttcgtgg
    21181 atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc ctgatgcgga
    21241 ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc ggtagtggtg
    21301 tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac aaacagtgca
    21361 gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct ttcttaagta
    21421 gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg
    21481 taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt
    21541 gatggagagt gatgagagaa tattacaaca cgatgatttt gcagagatta tgaagaacta
    21601 taccggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa
    21661 gaaagcaact taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga
    21721 gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt agggaataac acatactcta
    21781 catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa
    21841 acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc gagtggcgag
    21901 taatttgcga aaccacccga taacggagcg gcggtgttct cacgataccg acactctcac
    21961 cagatagcag cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc
    22021 caaagggcaa cgctttgcca agttccaacg tacagaccag cgcgccaaaa tgtctgacgc
    22081 tgaaatgggt aaacgtacca ccaggctcct gatggaacac cagcgcctcc agccccgccg
    22141 cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt aatacaccga
    22201 actgcggatg caaggagcca cgaattgcgg tatgtagatc aaggtgccaa cgcacagatt
    22261 ctttgccctg gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc cgacaggttt
    22321 ctccgctttc agcaaatagc tgccaacgac cgccaaacat tcggttcata tcgctatggc
    22381 aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagc aaccgccaac
    22441 gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc gtccagcatt tccacaggtg
    22501 ccgtctcatt accgtggatc cccgcagaaa tcaccagcgc gccttgcggt ggcgttaatg
    22561 gcgtcagttc cagcacacca tcgcccagcc agcgccagcg aacgccgttg atttctcgct
    22621 cggtgataac cggtttttta cccgttaagg tcagagcaag aaaattatcc attgccccct
    22681 ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa tacatccagt
    22741 gcttcccgcc cttcgcgcag caattgcggg tcggccagat cggcagcagt aagacgatcg
    22801 cggtagtaac gatccaccca gtcattgagc acattaaaca aggtatcgtt catcatcacc
    22861 gccggattca ccgcccggcg ttcttcttct gtcaataccc cccgcaaccg caggcacgcc
    22921 gggccgccgc cattcgccat gctttcacgt aagtcaaaga cttttagttc actaatcggg
    22981 ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgctc ccgacactcc
    23041 tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct gttaaacaga
    23101 taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat cgccataaaa
    23161 ccgttcaccc gcgtgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca
    23221 gcacctggcg gttactcacg gcaatcacgt cattatgaaa aacgctctgg tcgataacgt
    23281 ccgggttttg ctgggcgaaa atcacctgtt ggggattcac ctgattaagc cttgccaccg
    23341 cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt
    23401 cttctcgccc gtagacaaaa agtagcatac ccggttcacc gtaatgaccg ccgagacgat
    23461 tgtggtttgc cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatggacgc
    23521 taaatttctc ttcgtcctta aaaatcgctt ttaacagcga ttcagtgacg ggcgcttcca
    23581 gcgaacggtg aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg
    23641 tatcggcaga tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggaactga
    23701 cgctggaaag ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac
    23761 cactgaatcc caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg
    23821 cctgggggaa tcccgcatcg gcaagggctt tcattttcag taagccctgt tttgccgcca
    23881 gtcgcgggtt agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc
    23941 ccgcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg
    24001 caccacctca tcggaaaaat ccagcccggg gttaagcgtg gcgggcaatg ttaacgagtc
    24061 cgactccagg ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg
    24121 atggttaccg gaagcaccaa tgccgccgaa tggggcggta ctggcagcac cagtaagcgg
    24181 tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg
    24241 ctcgggggaa accagaccgc aaaagagtcc gaagcgagtg ttgttcgcca tccgaatcgc
    24301 ctcatcaaaa gtatcataac gccagacgcg caaaagcggc ccgaacacct cttcatccgg
    24361 cacaccgcca acgcccgtca tttcaatgat ccctggtgtc agcaacgatg tccctgcttg
    24421 taataagcgc ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt
    24481 aaccacctgc tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc
    24541 atcccagttg cccggcgtta atcgctggct gacggcaacc agactagcaa gaaacgcatc
    24601 gccctgcgct ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccgactgt
    24661 gacaaacgcc gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat
    24721 aattagcgga ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag
    24781 ctggcgatgc agctggtagc cggtattggc gctaccggta aacagcaaac cgtcgagatc
    24841 ctccagcgca ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc
    24901 cggcggcaag ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag
    24961 ttcgctgggt ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt
    25021 cggcaaatga ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg
    25081 tcgcaggctc gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata
    25141 cgccttaatt gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca
    25201 gcgcggctta cccgtttctc tggcaataat cgcggttaat tcggctttat tgctttccag
    25261 caatccggca aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg
    25321 cggaaacgcc gcacgggctg cccgacaagc ctgttcgacc tgagcggcat cggcatcatt
    25381 gccttgccat aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc
    25441 cgttatccag tcaccgttaa tccataaagt catgctgttt tctcctctgc gcacaggcgc
    25501 accagacgaa cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa
    25561 atcaaacgct cggttgccgg atcggtacgc gccagcacca cgcggaaatg gtgataattt
    25621 tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc
    25681 accagccgac ttttacggat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg
    25741 ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg
    25801 gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag
    25861 tgggtataga tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg
    25921 cagagaaaat cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag
    25981 aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta
    26041 aacttgtcgc gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg
    26101 ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga
    26161 tcgttactaa gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga
    26221 accaacgtgc cgacgcgata gttgtaccag ggatcgttca gcccaaccgc cacctcaata
    26281 gcgcaaatcc ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc
    26341 tcgcttttgg gcagttcacc ttgccaggtt ttgattgccc tttcgatacg cgccgaaagc
    26401 gtggcttcat tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc
    26461 agcgccgaga catctgagcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa
    26521 caaagtgttc gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga
    26581 cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc atcacgcctg
    26641 ctttcgccgc ttccagagag atctgtttcg cttgcccggc gtaatcggca ttcagtacac
    26701 agccaatcaa caaacctaag ccgcgaactt cactgaacaa accatagcgg tgattaatag
    26761 tattaagacg ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg
    26821 tgttgatgag ctccagcact ttgcctgcta ccgccgaggc cagcggatta ccgccatagg
    26881 tggtgccatg agtgccaacg gtcatcacgc gggcgcactc ttcggttgcc aacaacgcac
    26941 cgaccgggaa accgccgccc agcgctttgg cggtggttaa cagatcgggc gtcacgccgt
    27001 agtgcatata ggcatacagc tcaccagtgc gcccgacgcc ggtttgtacc tcatcaaaaa
    27061 tcagcaaggc attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg
    27121 ctggcaccac accgccttcg ccctagatgg gctcgacaat caccgcacag gtggcgtcgt
    27181 caatcagcgc gctggcagag ttaatatcgt tatatgcagc atgacgaata tccgccggca
    27241 gtggcgcaaa atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc
    27301 gaccatgaaa cgcatttttg aacgccacga tgccactctt atggctgccg tagcggtcgt
    27361 gagcgaattt acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa
    27421 agaagacgcg atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct
    27481 cgttggtgta accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac
    27541 gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat
    27601 actctttccc ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg
    27661 ccggagcgta aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca
    27721 tagcgacctc tacagtaaat aattcgttat ttgcatgtta acaataagta atgtttgcgc
    27781 tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg
    27841 ctaactggaa atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga
    27901 atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa
    27961 atataattct agaattgtga tcattgacga aatttactgg aaattactgc gccattctga
    28021 cgcagcgcgc accaaaagcg ggcatttttt gcgccatcat tgacatcatt aacaaccatc
    28081 gatcaaatca cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact
    28141 ctttatctga ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc
    28201 gcgccagacc tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc
    28261 tgcaggagac aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct
    28321 acaacgtgtt ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga
    28381 cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta
    28441 ttatggcgga aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc
    28501 agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc
    28561 tgcaaaacta cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata
    28621 tgaatatcag ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc
    28681 tgcgtaccgg taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga
    28741 gctggggggt ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat
    28801 ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc
    28861 tcgccagcca accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca
    28921 gcatggaaaa accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca
    28981 gctctcctgg ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca
    29041 gtaatgattt gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac
    29101 cacttctcta ttatcaagtt tgatatagga aactccacga tgaacgctga gcgtaacttt
    29161 ctgtttgcct gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc
    29221 gatctgctca ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggt
    29281 tatagcctct atattctgtt attcatcaat gccccctctt tctgttacca ggaagcatac
    29341 tggtgatcgc tggaggaata gtttttggcc cgctcttagg acacaactct cattaattgc
    29401 cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac gtgatttact
    29461 gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca ttgcgcgtaa
    29521 cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca atattcaaaa
    29581 ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt cggcactcac
    29641 gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca atgaaggcat
    29701 tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta ttctcatcca
    29761 gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc gccgcagccc
    29821 acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca gtgtcatgga
    29881 aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc tgcgatcaaa
    29941 aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa ccgctgcgcg
    30001 tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt ttcctcaccg
    30061 acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa tcgctgttta
    30121 tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt ggtacgccgg
    30181 gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc aaactggata
    30241 tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg attgttgaca
    30301 gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca gaagagaaga
    30361 aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc aatgcaacat
    30421 acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat gcctcagttc
    30481 tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa agccaagata
    30541 aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc tcatcatcct
    30601 gttattcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt ttatcaatcg
    30661 cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta ttcagtctta
    30721 cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca ttgctgcacc
    30781 gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt tctggggcgg
    30841 cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta tcgccagagt
    30901 gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca gtatggacgg
    30961 ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc cttttgtccc
    31021 tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct cgttttttat
    31081 cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag gcagcatgtt
    31141 aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc tgaccgtggt
    31201 gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact gatgggttta
    31261 ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca tcgtcgccat
    31321 tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat cttttatttg
    31381 gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc ggtagtgcga
    31441 tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt ggatatcacc
    31501 agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt ggctgactgg
    31561 cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc cgaagccgca
    31621 agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc gcattttgac
    31681 gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc ggctcgtttt
    31741 aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa acgttcttaa
    31801 ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt ttctttggca
    31861 acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac cgtctggttt
    31921 aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag cggcgagatg
    31981 aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc agacgcggtg
    32041 aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt ggatctgctc
    32101 tgggtgaacg gcgaaaactt cc
//
LOCUS       DBKELA010000039        31765 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.039, whole genome shotgun sequence.
ACCESSION   DBKELA010000039 DBKELA010000000
VERSION     DBKELA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.039"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="ACUQR0_001803"
     CDS             complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="ACUQR0_001803"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HHV9108880.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1793..2539
                     /gene="mipA"
                     /locus_tag="ACUQR0_001804"
     CDS             1793..2539
                     /gene="mipA"
                     /locus_tag="ACUQR0_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HHV9108881.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2629..3390
                     /locus_tag="ACUQR0_001805"
     CDS             2629..3390
                     /locus_tag="ACUQR0_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHV9108882.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3441..4325)
                     /locus_tag="ACUQR0_001806"
     CDS             complement(3441..4325)
                     /locus_tag="ACUQR0_001806"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HHV9108883.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="ACUQR0_001807"
     CDS             complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="ACUQR0_001807"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHV9108884.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5746..6159
                     /gene="msrB"
                     /locus_tag="ACUQR0_001808"
     CDS             5746..6159
                     /gene="msrB"
                     /locus_tag="ACUQR0_001808"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HHV9108885.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6201..6473
                     /locus_tag="ACUQR0_001809"
     CDS             6201..6473
                     /locus_tag="ACUQR0_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HHV9108886.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6843..7919
                     /locus_tag="ACUQR0_001810"
     CDS             6843..7919
                     /locus_tag="ACUQR0_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HHV9108887.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7946..9325
                     /locus_tag="ACUQR0_001811"
     CDS             7946..9325
                     /locus_tag="ACUQR0_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9108888.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9342..10387
                     /locus_tag="ACUQR0_001812"
                     /pseudo
     CDS             9342..10387
                     /locus_tag="ACUQR0_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10408..11244
                     /locus_tag="ACUQR0_001813"
     CDS             10408..11244
                     /locus_tag="ACUQR0_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HHV9108889.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11249..12196
                     /locus_tag="ACUQR0_001814"
     CDS             11249..12196
                     /locus_tag="ACUQR0_001814"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HHV9108890.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNEALDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12206..13186
                     /gene="ydjG"
                     /locus_tag="ACUQR0_001815"
     CDS             12206..13186
                     /gene="ydjG"
                     /locus_tag="ACUQR0_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019170 - methylglyoxal reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HHV9108891.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13323..14081
                     /locus_tag="ACUQR0_001816"
     CDS             13323..14081
                     /locus_tag="ACUQR0_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHV9108892.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14199..15557
                     /locus_tag="ACUQR0_001817"
     CDS             14199..15557
                     /locus_tag="ACUQR0_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9108893.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="ACUQR0_001818"
     CDS             complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="ACUQR0_001818"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HHV9108894.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="ACUQR0_001819"
     CDS             complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="ACUQR0_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HHV9108895.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="ACUQR0_001820"
     CDS             complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="ACUQR0_001820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HHV9108896.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19502..20053
                     /locus_tag="ACUQR0_001821"
     CDS             19502..20053
                     /locus_tag="ACUQR0_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HHV9108897.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20170..21213
                     /gene="selD"
                     /locus_tag="ACUQR0_001822"
     CDS             20170..21213
                     /gene="selD"
                     /locus_tag="ACUQR0_001822"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HHV9108898.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVHARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21218..23179
                     /gene="topB"
                     /locus_tag="ACUQR0_001823"
     CDS             21218..23179
                     /gene="topB"
                     /locus_tag="ACUQR0_001823"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HHV9108899.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23306..24346
                     /locus_tag="ACUQR0_001824"
     CDS             23306..24346
                     /locus_tag="ACUQR0_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HHV9108900.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24463..25806)
                     /gene="gdhA"
                     /locus_tag="ACUQR0_001825"
     CDS             complement(24463..25806)
                     /gene="gdhA"
                     /locus_tag="ACUQR0_001825"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HHV9108901.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26042..26314
                     /locus_tag="ACUQR0_001826"
     CDS             26042..26314
                     /locus_tag="ACUQR0_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HHV9108902.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26280..26687)
                     /gene="nudG"
                     /locus_tag="ACUQR0_001827"
     CDS             complement(26280..26687)
                     /gene="nudG"
                     /locus_tag="ACUQR0_001827"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HHV9108903.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26774..27394
                     /locus_tag="ACUQR0_001828"
     CDS             26774..27394
                     /locus_tag="ACUQR0_001828"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HHV9108904.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27403..28710)
                     /gene="ynjE"
                     /locus_tag="ACUQR0_001829"
                     /pseudo
     CDS             complement(27403..28710)
                     /gene="ynjE"
                     /locus_tag="ACUQR0_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28777..29430)
                     /locus_tag="ACUQR0_001830"
     CDS             complement(28777..29430)
                     /locus_tag="ACUQR0_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHV9108905.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29430..30947)
                     /locus_tag="ACUQR0_001831"
                     /pseudo
     CDS             complement(29430..30947)
                     /locus_tag="ACUQR0_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(30920..31765)
                     /locus_tag="ACUQR0_001832"
                     /pseudo
     CDS             complement(30920..31765)
                     /locus_tag="ACUQR0_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7957 a         7605 c         8240 g         7963 t
ORIGIN      
        1 gctgttccag gacgtagaac agatggaccg ggtttgccgc cacttctaca tgatcgaagt
       61 tgaacacgcg ggagaggatc ttaaacgcaa aacgcgtcga cagaccgttc atcccttcat
      121 cgacaccggc gtagtcacga tattcctgat acgacttggc tttcggatcg gtgtctttca
      181 gactttcgcc atcataaacc cgcatctttg aataaatgct ggagttttct ggctctttca
      241 ggcgcgaaag aatggaaaaa cgtgacagtg tttcgagcgt gccaggggcg catggggcgt
      301 gagtcaattc actgtgatta agcaatttct cgtagatttt gatctcttcg gaaatgcgca
      361 agcaatacgg caccttcacg atgtaaacac ggtcgaggaa ggcttcgttg tttttgttat
      421 tacggaaagt gacccattcg gacttgttcg agtgggcgag aataatcccg ttgaacggca
      481 gggcggagat accttccgtc ccgttgtagt taccttcctg agtggcggtt aacaagggat
      541 gcagcacttt tatcggtgct ttaaacatct caacgaattc catgatcccc tgattggcgc
      601 ggcacagcgc accggaatag ccgtaggcgt ccgggtcatt ctgcgcgtag tgttcgagtt
      661 tacgaatatc gactttccca accagcgcgg agatgtcctg gttgttctca tcaccgggtt
      721 ccgttttggc gatagcaatt tgttgcagaa ttgacggcca gaccttcact acccggaact
      781 tagtgatatc gccaccaaat tcatgcaggc gttttgccgc ccatggcgac atgatggtgc
      841 cgagataacg gcgaggaatg ccatactctt tttccagaat ctgagcatct tcctgcggat
      901 tgaaaagaca gaacggatga tcgttgaccg ggctacgctc accgttggcg ctcaatacat
      961 aaatcggtac gagctgcatt aatgatttca gtcgctcagc aagcgatgat ttacccccac
     1021 ccacaggccc cagcagatac aggatttgtt tcttctcttc cagcccctga gccgcgtgtt
     1081 tcagataaga gacaatctgt tcaatcgcgt cttccatgcc gtaaaactct tcaaatgccg
     1141 gataacgtgc aatgacccgg ttagaaaaga gtcgagaaag tctggggtcc tgggctgtat
     1201 cgaccatgac aggctcaccg atagccatca atagccgctc agccgcgttg gcataagcac
     1261 tgcgatcttg ccgacaagtg gtaagaaact cctgcagtgt gaactcttcg tccttggcag
     1321 cttcatatcg ctggcgatag tgatcaaata tattcatggt atgccgtcct ttcgtttttt
     1381 agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg taaactgaac
     1441 gacgtctctt gctaaaacac agcaactctc atgccatttt atcgacgatg agaacaacgt
     1501 gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg
     1561 ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa
     1621 agaccaggct cgccagtaac gctaaatcca tttggctgta agcgcggtgt catccgcggc
     1681 aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat
     1741 tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa
     1801 actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga
     1861 aggtaaattt tccctgggcg caggcgtagg tgtcgttgag cacccatata aagattacga
     1921 taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg
     1981 cttaggtggt ggttactacc tgtggaatga cgcaacggat aaactttcaa ttaccgctta
     2041 ctggtcgccg ctttacttca aagcgaaaga cagtggcgat caccaaatgc gtcacctgga
     2101 tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta
     2161 cctgcgtacc accctggctg gcgataccct ggataacagc aacggcattg tctgggatat
     2221 ggcctggttg tatcgttaca ccaacggtgg cctgaccgtg actccgggta ttggtgtgca
     2281 gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg
     2341 cagcggtctg cgtggctata acccgaatga cagctggagc ccttaccttg agctgagcgc
     2401 cagctacaac ttcctcggcg actggagtgt ttacggtacc gcgcgctaca cccgtctgtc
     2461 tgatgaagtt actgacagcc cgatggtgga taaatcctgg actggcctga tttctaccgg
     2521 gatcacctac aaattctgat aatgcatctt tccagggcat tttgtgcata aaaacagggc
     2581 gtcacgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa
     2641 atgattcaat ttagtggcga tgtctcactg ccagccatag ggcagggaac atggtatatg
     2701 ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac tacgcgcagg cattgaactc
     2761 ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt
     2821 ggggaagcat taaccggtct gcgagagaag gtctttctcg tctctaaagt ctatccgtgg
     2881 aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact
     2941 gactatctcg atctctactt attacactgg tctggcagtt tcgcttttga agagactgtt
     3001 gccgcgatgg aaaaattgat cgcccaggga aaaatccgtc gctggggcgt ttctaacctt
     3061 ggttatgccg atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat
     3121 caggtgcttt accatctcgg ttcacgaggg attgagtacg atctactccc ctggtgccag
     3181 caacagcaga tgccggtgat ggcttacagt ccattagccc aggccgggcg gttgcgcaat
     3241 ggactgttaa aaaacgcggt agtcaacgaa attgcacatg tccaacaaca tgcagctgcg
     3301 cttgaggtcg aactttcgtc agcggaatta gctatgctgg ataaggcata tccggcacca
     3361 aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg tgccggatgc atcgcacccg
     3421 gcacacaaca ttaacgcaaa ttaacgtttg gcaacgcgaa tggattgcgc cagatgtgca
     3481 ggtttctctt tggtgacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaag
     3541 gtcttgtagc catcgtccgg catgtcgccc atgctaactg aaagcgccgg tcccgggttc
     3601 cagccgacaa cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttca
     3661 tcattaatca cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag
     3721 gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat tcactttatc aatgaagcga
     3781 tcgcccagcc cactgacgct taccttagcg atatcaccca cgttaaagta ggtatgcagg
     3841 gcagaggtgg tttcaaattc gccatgtgat tcaagatcga tttcacaggt tttacccacg
     3901 cggaaatgcg ctaacagcgt aaagtcgtgc ggccagaatt ttttcgtctc ttcgctttgt
     3961 gtcaattcaa aagtcagcgc tacgccatca gcatcttcat gatgtgattt cagcgtccac
     4021 ggcaggttgc gggcaaaacc gtgcgcaggc agaccttgtt gtgccgccgg accaaaccac
     4081 ggccagcaaa ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttgttg
     4141 ctcaaccaca gaacttcttc ttcacccgca ggtttccacg agagaaggtg tgcgccctgt
     4201 aatgcaaaag aggcttttac ctggggatga tcgaccacaa tgaggtccag ttcatccagt
     4261 ttacgacggg agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta
     4321 atcatgacgc agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc
     4381 tctttttaga tcacagtgtc atctcaactt atttggagat gtgagcgatc aggtccagaa
     4441 ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt
     4501 tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg
     4561 tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcag
     4621 ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt
     4681 caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca
     4741 gttctggcag tactttacct acagctttag cagcaccggt agaggacggg atgatgttct
     4801 gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag
     4861 tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga
     4921 taactttagc cagcggagct aggcagttgg tggtgcagga agcgttggaa acgatgtcct
     4981 ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg
     5041 gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag
     5101 tcaggaacag accagttgct tcagcgacaa cgtcaacacc cacttcgtcc catttcaggt
     5161 tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt
     5221 ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg
     5281 ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct
     5341 gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag
     5401 tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac
     5461 cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag
     5521 cattacgcga ctgactcgcc tcacttttcc tttgggcttg agaccacatg gggtcggcgc
     5581 cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc
     5641 catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag
     5701 aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agtaaatggc gaataaacct
     5761 tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac gcagaatcat
     5821 gggacagaac cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac
     5881 tgtttgatct gcgatgcccc gctgtttcat tccgaaacca agtatgattc cggctgtggc
     5941 tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa agacttgtca
     6001 catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct ggggcatgtc
     6061 tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg ttaactctgc ttctttacgc
     6121 ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat
     6181 tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg
     6241 tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc gttgcgttga
     6301 ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct gtggcaagcc cgccataata
     6361 ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc
     6421 agttaaaaga agattttggt atctcagcaa agcctattgc catgtttaag taagtggttc
     6481 atcccctttt tgccgcggct atgaagggga tagccaacaa tgaccccacg actcgtctca
     6541 attatctcga cccgacctgg ctaagtccca gtccgatctc ttgcagtcgc gttcacgcct
     6601 gaatgacttc tcctgaactt aagtctcaga cctatttggc cggtaatccc tctcgaattt
     6661 gctctttttg cgtcccgcct tccaccaacc tgtaacagac ccagccattt tctgcgtaat
     6721 tgtgatctga gttgtaaaat gcaggtcatt atctgtgttg tgcagattta atattgacct
     6781 caaaaatcat gtgggtaagt atgatggcca tctaagcaaa acaaaacaca gaggatgacg
     6841 caatgaaagc actggctcgg tttggcaagg cctttggcgg ctacaagatg attcatgtcc
     6901 cacaacccat ttgtggcccg gaagacgtag tgattgaaat taaagccgcg gcaatctgcg
     6961 gcgcagacat gaagcactac aatgtcgata gcggttctga tgagtttaac tctatccgcg
     7021 gccatgagtt cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac tggaaagtgg
     7081 ggcaacgcgt cgtgtcggat aacagcggtc acgtttgcgg cgtttgtccg gcctgtgaac
     7141 agggtgattt tctgtgttgt acagaaaaag taaaccttgg tctggataac aatacctggg
     7201 gcggtggttt ttccaaatat tgtctggttc ctggtgaaat tctcaaaatt catcgtcatg
     7261 cgttgtggga aatccctgat ggtgttgatt atgaggacgc agccgtactt gaccctatct
     7321 gtaatgccta taaatccatc gcgcagcaat cgaaattcct tcctggtcag gatgttgtcg
     7381 tcattggcac tggcccactc gggctgtttt ccgtacaaat ggcgcggatt atgggggcgg
     7441 taaatatcgt cgtcgttggt ctgcaagaag atgtggcggt ccgcttcccg gttgcaaaag
     7501 aactgggggc gacggcagta gtaaatggtt ctaccgaaga tatggtggcg cgctgccagc
     7561 aaatttgtgg caaagataat ctgggactgg tgattgaatg ctccggtgcc aatatcgcac
     7621 tgaaacaagc catcgaaatg cttcgcccga atggggaagt ggtacgcgtt ggaatgggct
     7681 tcaaacctct tgatttctcg attaatgaca ttaccgcctg gaataaaagc atcattgggc
     7741 atatggccta tgactccacc tcatggcgta acgctatccg gctattagcc agcggcgcta
     7801 tcaaagtcaa accgatgatc acgcatcgta tcggcctgtc gcaatggcgc gaagggtttg
     7861 atgcgatggt cgataaaacc gcaatcaaag tgatcatgac ttacgacttt gatgaataac
     7921 tactctcctg aaggaacgtt atcgcatgga acagataaca aaaccgcatt gtggtgcacg
     7981 gctggatcgc ttaccggatt gccgctggca ttcgtcaatg tttgccatcg tcgcgtttgg
     8041 cttgcttgtc tgctggagta atgccgttgg tggcttgatc ctcgcgcagc tgaaagcgtt
     8101 gggctggaca gataattcca ctacagccac attctcagca atcacgaccg ccggaatgtt
     8161 tctcggtgcg ctggtgggcg gcatcattgg cgacaaaacc ggtcgcagaa atgcgttcat
     8221 cctctatgag gccattcata ttgcctcgat ggttgtcggt gctttctcac cgaatatgga
     8281 ttttcttatt gcctgccgtt ttgtgatgga cgttgggctg ggagctttac tggttacact
     8341 gtttgctggt ttcaccgaat atatgcccgg tagaaatcgc ggaacgtggt ccagtcgggt
     8401 ttcctttatt ggcaactggt catatccgct ctgctcactg attgcgatgg gactcacgcc
     8461 gctgattagt gcagagtgga actggcgggt acaactgctt attcctgcaa tattgtcgct
     8521 tatcgctacg gcgctggcct ggcgctactt tcctgaatcc ccgcgctggc tggaatcgcg
     8581 cggacggtat caggaagccg agaaagtcat gcggagtata gaagaaggcg tcatacgcca
     8641 gacgggtaaa cctttgccgc ctgtggttat tgctgatgac ggtaaagcgc cacaagcggt
     8701 gccgtattca gccttactga caggagtatt actgaaacgc gtgatattag gctcttgtgt
     8761 gctgattacc atgaacgttg tgcagtacac actaattaac tggttgccaa caatattcat
     8821 gacccagggg attaatttaa aagactcgat tgttttaaat accatgagta tgtttggcgc
     8881 gccatttggt atttttattg ccatgctggt gatggataaa attccgcgta aaacaatggg
     8941 tgtggggcta ttaatcctga ttgcggtgct cggatatatc tattcactgc aaaccagtat
     9001 gttgctcata acgctgattg gtttcttcct gattactttc gtctatatgt acgtttgcta
     9061 tgcctcggca gtgtatgtcc ctgaaatctg gccgacagag gccaaactcc gtggctccgg
     9121 tctggcgaat gcggtagggc gaatcagtgg tattgccgca ccttatgccg ttgcagtgct
     9181 gctcagtagt tatggtgtaa cgggagtctt tattcttctg ggggctgttt caaatattgt
     9241 cgcaattgct atcgccacca ttggaattga aaccaaaggt gtctccgttg aaagtttaag
     9301 tattgatgca gtagccaata aataatacgg gtggattcaa aatgaaaaat tcaaaagcaa
     9361 tattgcaggt gccgggcaca atgaaaatta tttcagcaga aataccagtg cctaaagaag
     9421 atgaagtttt gattaaagta gaatatgtcg gtatttgtgg ttcagatgta catggttttg
     9481 aatcaggccc gtttattccg cctaaagacc caaatcaaga aattggcctg ggtcatgaat
     9541 gcgccgggac ggttgtggct gtgggaagcc gcgtgcgcaa atttaaaccg ggggatcggg
     9601 taaatatcga acctggcgtt ccttgcggtc actgtcgtta ctgtctggaa ggcaaatata
     9661 acatctgccc ggacgttgat tttatggcga cacaacccaa ctaccgcggc gcattaacgc
     9721 actatctgtg tcatccggag agctttactt acaaactgcc cgacaatatg gacacgatgg
     9781 aaggggcgct ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg gcagatgtta
     9841 aaccgggtaa gaagataatt attctgggag caggttgtat tggtttgatg acgttgcaag
     9901 cgtgcaaatg cctgggcgca acggaaattg ccgtcgttga tgtactggaa aaacgtctgg
     9961 caatggggga acagcctggt gcgacagtgg ttattaatgg cgcaaaagag gacactattg
    10021 cacgctgtca gcaatttacc gaagacatgg gcgcagatat tgttttcgaa acagcgggtt
    10081 ctgcggtcac cgttaaacag gcacctttaa tctggtaatg cgcggcggta aaattatgat
    10141 tgttggtact gtacccggcg attcggcaat caatttcctc aaaatcaatc gcgaagtcac
    10201 tatccagacg gtattccgct atgccaatcg ttatccggtc acgattgaag ctatttcttc
    10261 agggcgattc gatgtgaaat cgatggtgac gcatatttac gattatcggg atgtacaaca
    10321 ggcatttgaa gagtcagtta acaacaaacg cgacattatt aaaggcgtta ttaaaattag
    10381 cgattaatta atacgggaga acagaacatg ctcgcagata tcagatattg ggaaaacgat
    10441 gccacaaata aatattatgc aattgcccat tttaacgtat ggaatgcaga aatgttgatg
    10501 ggcgttaaag acgcagccga agaagcgaaa tccccggtta ttatttcttt tagtacaggt
    10561 tttgtcggta acacctcatt tgaagatttc tctcacatga tggtatcaat ggcacaaaaa
    10621 gcaacggtgc cggtaataac tcattgggat catggtcgga gtattgagat tattcataac
    10681 gcctggactc atggtatgaa ttcattaatg cgtgatgctt ctgcatttga tttcgaagaa
    10741 aatattcgtt taaccaaaga ggctgttgat ttctttcatc cgctgggtat tccggtagag
    10801 gcggaattag ggcatgtcgg taatgaaacc gtttatgagg aagcgctggc gggctatcac
    10861 tataccgatc ctgaccaggc ggctgaattt gttgaacgta cgggctgtga ctcattggct
    10921 gtcgccatcg gcaaccagca tggggtttat acgtcagagc cgcaattgaa ctttgaggtc
    10981 gtcaaacgcg tacgcgatgc tgtttctgtt ccgctggttt tgcatggcgc atcggggatc
    11041 agtgatgccg acattaaaac tgcaatttcg ttgggtattg cgaaaatcaa cattcatacg
    11101 gagctctgtc aggccgcaat ggtagcagtg aaagaaaacc aggatcagcc tttcctgcat
    11161 ttagaacgtg aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat tgaactgttc
    11221 ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt gtataggtgc
    11281 cgctattgtt gatattccat tacaaccggt cagtaaaaat atctttgatg tagattctta
    11341 tcctcttgaa agaatcgcaa tgagcaccgg gggcgatgca ataaatgaag caacaattat
    11401 ttctcgtctg gggcatcgta ccgcattaat gagccgcatt ggtaaagatg cctcagggca
    11461 atttattctc gaccattgtc gcaaagagaa tattgatatt caaagcctga aacaggacgt
    11521 cagcatagat acatctatta acgttggact ggtgacggag gatggcgagc ggacgtttgt
    11581 cacgaaccgt aacggcagtt tgtggaaatt aaatattgac gatgttgatt ttgcgcggtt
    11641 tcctcaggcg aaattattat cactggccag tattttcaac agtcctctat tggatggtaa
    11701 agcgctaaca gaaattttta cgcaagcgaa agcccggcag atgatcattt gtgccgatat
    11761 gatcaaaccg cggttgaatg aagcgctgga tgatatttgc gaagcattga gttatgtcga
    11821 ttatctgttt ccaaattttg ccgaggaaaa attactcaca gggaaagaga cactggatga
    11881 aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa cgggtaaaga
    11941 cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg caggaataac
    12001 cgccatcgac accattggcg cgggcgataa ctttgcttca ggttttattg cggcactgtt
    12061 agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg ctatctcggt
    12121 tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac aattgctgga
    12181 agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacga cggatataac
    12241 gctttcgcga atggggttgg ggacatgggc cattggcggc ggtcctgcat ggaatggcga
    12301 tctcgatcgg caaatatgta ttgatacgat tcttgaagcc catcgttgtg gcattaatct
    12361 gattgatact gcgctaggat ataactttgg caatagtgaa gttatcgtcg gtcaggcgtt
    12421 aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg tctgggaacg
    12481 aaaaggaagt ttattcaaca aagttggcga tcggcagttg tataaaaacc tttccccgga
    12541 atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt acatcgatat
    12601 ctatatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg aaactgtcgc
    12661 agtgcttaat gagttaaagg ctgaagggaa aattcgcgct ataggcgctg ctaacgtcga
    12721 tgctgaccat atccgcgagt atctgcaata tggtgaactg gatattattc aggcgaaata
    12781 cagtattctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg ataatggcat
    12841 tgtggttcag gtttattccc cgctagagca gggattgttg accggcacca tcactcgtga
    12901 ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa acatgctgaa
    12961 agtgattgat atgcttgaac agtggcagcc actttgtgct cgttatcagt gcacaattcc
    13021 cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc ttagtggggc
    13081 tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg aatatcaact tatcggatgc
    13141 agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac tcaacacgat
    13201 tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaatagg ttattttgaa
    13261 agtaaacatc ggttatgcga taatcgcgct aatgtgatgt aaaaagatac aggggtgtaa
    13321 tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac gataaaaagg
    13381 tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt cgccgcgatc
    13441 ttgagaagct ggaagatgaa ggctttttga ccagaaccta tggtggtgct gttttaaata
    13501 cagcggtgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt tatgaagaga
    13561 agctgcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc atggcagccg
    13621 attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt gacctgacgt
    13681 tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt aaagtcgttt
    13741 caacgggtgg ggaactaaac aaaaatacac tttccctgca aggaagaatt actaaagaga
    13801 tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt gatattaaca
    13861 gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg atccgtcagg
    13921 cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgtaaagct tttgtccagt
    13981 tagctgactt tagtcatatt aattatataa taactgataa atcaccgggt gcagagtgga
    14041 tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac acgttgtcaa
    14101 tattatttgt tcctgcgact ggaaataaaa tgatgataaa gatgcggcaa gtgaatttgc
    14161 cgcaattgct accttttata gcaaatccga ggcacccaat ggaacaatat gatcaaattg
    14221 gcgcaagact ggaccgcttg cctttggccc ggtttcatta tcgtatattt ggtataataa
    14281 gctttagtct gttattaacg gggtttttga gttactcagg taatgtcgtc ttagcaaagc
    14341 tggtaagcaa tggatggtca aataatttcc tctatgccgc ctttacctcg gcattaatgt
    14401 ttggttattt catcggctca cttactggtg ggtttattgg tgactacttt gggcggcgca
    14461 gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc aacaggggcc gcttttgtcc
    14521 ctgatatgta ctggctcatt ttctttcgct tcctgatggg aacaggaatg ggggcgctga
    14581 ttatggttgg ctatgcctca tttacggagt ttatccccgc gacggtgcgt ggaaaatggt
    14641 ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct gtctgcggcg ataggcgtgg
    14701 tggttatcgc tttttttagt tggcgaataa tgtttcttct gggtggtatt ggcatactgt
    14761 tagcctggtt tctctcaggt aaatacttta tcgagtcgcc acgatggctg gaagggaaag
    14821 ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga gcagcaaatt gaaagggaga
    14881 agagtattcg tttaccccag cttactttga accagagcaa cagcaaggtt aaggtaatca
    14941 agggtacctt caggctcctg tttaaagggg aaatgttacg acgtacatta gttgcgataa
    15001 ctgttttaat tgcaatgaac atttcgcttt ataccattac cgtatggata ccgaccatat
    15061 ttgttaactc cggcattgat gtcgataaat caatattaat gaccgctgtt attatgattg
    15121 gtgctccggt aggaatattt attgcagcat taattattga tcattttcct cgtcgattat
    15181 ttggctccgc cttacttatt attattgccg tgttaggcta tatctattca agtcagacta
    15241 cagagtgggc gattttaatc tatggtctgg tgatgatctt ctttttatac atgtatgttt
    15301 gcttcgcgtc ggcggtttat atcccggagc tttggccaac gcatttacgc ctgcgcggtt
    15361 cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt cacgccctat ggcgttgcgg
    15421 cattattaac acattatggg tcgatcacgg tgtttatggt gcttggtgtc atgttagtgc
    15481 tctgtgcgct ggttctctcc atttttggca tcgaaacgcg gaaggtgtcg ctggaagaga
    15541 tttctgaggt gaattaaatc tttcgttgat ggcatcaatt gccagatgta ccactgacgt
    15601 gttttatttg gcctacaatg ccagtacaga attgtaggcc agcgtaaaat tacccctgcg
    15661 tctcttccca gtctgccagc gtatatagcg ttgccccagc tgctgacatc tccataaacg
    15721 cgtgtgcact gtcctggggc tggatattca cgccacgaca gccatcggta atcacgttta
    15781 ccttataacc taactgtaac gcgtccagca cggtaaactt cacgcaatag tcagtagcca
    15841 ggcccataac gatcaattca tcgatttcat gatcgcgcaa ccagtcatca agcgcggttt
    15901 tctgccgacg tccgttatca aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt
    15961 tatggaacac cgctgcgatc tctttttgat tcagtaacgg atgtaattgt gcgccttcac
    16021 tgttctgcac acagtgttca ggccagaaag tttgccgcag gccgccgagt tggcctggcg
    16081 tataaggctc tacgccgtgc tggctggcaa agctgccgtg attcgccgga tgccagtcct
    16141 ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc aataaggcgg ttagcaacct
    16201 ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc agcacagaaa tcattttgta
    16261 aatcgaccag taacagggcg cgatggggca ttgcagtctc cttaatcatc cggcgtcagt
    16321 tcgccgcgca ggttttggct catggccttg cgaatggttt cagtatccag ttcctggctc
    16381 agcaggtaat gcagtttggt tagcgtggct tctacagtca tatctgcacc gtcaataacg
    16441 ccggcatggg cgagggcgtt accggtggca taaccactca tattcacttt accggacata
    16501 cattgtgtca ggttgaccac cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg
    16561 aaggctttgt tttgtggcgc attacccacg ccataggagc gcagaatcaa tgctttcacc
    16621 ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc ctggataaat cgtcaccacg
    16681 ccaattggtt gtggcgtgat tggatgaacg attaattccc cttcaccgtg cggggcgggt
    16741 ggcgtattca aacgacgaat atggatacca gcttccagta acggaggaag gtttggagag
    16801 gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg
    16861 ttattgaaaa agagcgttac ttcgttgatc ggataattcg ccgcaacgta caacgcattc
    16921 agcagattaa tttgtccgtc agagcgtaac tcagccagcg ggatttgtga ccctgtcaca
    16981 atgaccggtt taccgagatt ctcgagcatg aacgacagcg cagaggcggt atacgccatc
    17041 gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc tttaatatct
    17101 tcagcaatat gctgccagtc ttccggcgtc atatcagaag aatccatcaa cggcgtatat
    17161 tcatgaatgg tgaaatctgg catctccggg cgatggaatt ccggcatcag cgccagttgg
    17221 cgttgtagat gacctgacac cggaatataa ccctgctcgg aacgctgcat cccgatggtc
    17281 ccgcccgtgt aggcgacgta aattgatttc ttttgcatga tattgatatg aatgatatcg
    17341 aaagagcaaa agtatatacg tgaggcagga ggagcgcacc tgatactatg cggataagga
    17401 aatatggggc gagacagcgg gaagaggatt cgtcgaggta aaaaataatg tggccgacga
    17461 tctgtcggcc actcagtaca agacttaacg cacgttggcg caggtcaggc aaaacgcata
    17521 acggttttgt gggtcgttaa acgcggccag cttgtcgctt tcacttttaa cggtagaggc
    17581 taccgaggca agcggtgcag gtaacatggc ctggaacgca tctggcaaca ttgcccggac
    17641 agaaccagac atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc
    17701 cagatgccac tgtttcactt ttgcgagctc tgctgctttg gcaaccgcat catcgaaatc
    17761 cccgagacta tcgaccagcc cgttagcttt tgcatcctga ccggtccaga cgtggccctg
    17821 ggcgatttta tcaatttgct ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa
    17881 gcgtttatag ccattctcaa tgcttaattg catcatctgc tgcgcttccg gcggcagtgc
    17941 cctggtgata gaaacatccg ccagcggtga agttgagaca ccatcagtat gaacaccaat
    18001 cgaatccaga ctattttcta cggtggtgat cacgccgaag ataccgatag aaccggtcag
    18061 ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa atccagtaac caccagatgc
    18121 cgccatgccg cccatcgata caaccacaga cttacccgct gcccgggctg ctgccagttc
    18181 agcgcgaatc acttcagacg cggtaacgct gccgcctggg ctattaacac gcaggacaat
    18241 cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc
    18301 aacattcccc tgagtttcct cgccatccat aattgcgcca ttagcgaaga cgacaccgat
    18361 gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg taataactga tggcgcgata
    18421 atttttatca gtcttactcc agccgaactc tttggtcaga gctttttcga tttcggcact
    18481 cgatgccagt gcatcgacca gcttgttttc cagtgcatat ttcgcggtat cgccaccggt
    18541 tttggttaaa ccctcaagca acccttgcgc gccagggaat acctgctgag cagggatctg
    18601 ccggttagcg gcaacagtat tcagatagtt ttgccacagc tcaccaatcc agcggctgtc
    18661 agcttcgcgg gctgccggtg acatatcatc acgaataaac ggttcaacgg cagatttata
    18721 cgtacccacg cggaacacat gggtggaaac tttcagctta tccagcaacg atttgtagta
    18781 cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca gccaaatttt
    18841 attggcgaaa ctggcgagat aatattgccc ctggctgtag ttctcgccaa cggcataaac
    18901 cggtttcccg ctgtcacgaa actctttcag ggctttgccg atgtactgca tagacggctg
    18961 gtcgccgcct gcgaagtttt tcagatccat cacgataccg gtgatattgc ggtcgtcctt
    19021 cgcctggcga atggtgttga cgatatcaaa cagtgagttt tcctgcagac gatcggaact
    19081 ggcaccaagc agctggcggc ttaatttact aaaccgctga gaactgtctg gtttatcgac
    19141 gatcacacca gaaatgtcca gcagcagtgc gccacgactg gccgtttctt tcgaatcacc
    19201 accactgacc tgcatccaga tccccacacc aaccagtacg aggaaaataa agaacaggtt
    19261 aagtaccatt tcacggacga aattcagcag acgccacgtc catttaaaaa atccggcaat
    19321 aaatcgccaa agggttcgca tgtattctcc caacttaagg tcacacctgt cacaggcgca
    19381 atagatgtgg ttatcgtaat gacccaaccg gcacttgtca gcaggaattg cggactgtgc
    19441 tgtaacaaaa tccactctcg tgttaatttt gtgaataaat atcacgacag gagttaatca
    19501 aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc
    19561 gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa
    19621 gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa gggtgcgagc gtttcagcgc
    19681 cgtactggaa cagggggcga ttgctgccgg tagtgatgat aaagctatcg acaaagcccg
    19741 taatgcgccg ttccgcgcac cgctcatcat cacggtggtg gcgaaatgca aagaaaatca
    19801 taaagtcccg cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat
    19861 ggcagccgtt gcccagggat ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc
    19921 ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa attgtcggtt ttctctacct
    19981 cggcacgccg cagttgaaag catctacgtc gattaacgtc ccggacccga cgccgtttgt
    20041 aacttatttc tgataattgt cgcgaaactg tctggattgt gagcaaaggc gcaggaattc
    20101 agacactctc acttatcact tcacggaatg agggttacca tacccggatt gcattgacag
    20161 gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat acagccacgg agctggttgc
    20221 ggctgtaaaa tttccccaaa agtgttggaa accatcctgc acagtgagca ggcgaagttt
    20281 gttgatctga atttgcttgt gggtaatgaa acccgcgacg atgcggcggt gtacgatctg
    20341 ggcaatggca ccagcgttat cagtaccacc gacttcttta tgccgatcgt tgataatcct
    20401 ttcgattttg gccgcattgc ggcgactaac gccatcagcg atatcttcgc gatggggggc
    20461 aaaccgatta tggcgattgc gatcctcggc tggccgatta acaaactttc cccggaaatt
    20521 gcccgcgaag taaccgaagg tggacgctat gcatgccgtc aggcgggtat tgcgctggct
    20581 ggcggtcact ccatcgatgc gccggagccg atttttggtc tggcggtaac ggggatcgta
    20641 ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg gatgcaaact gttcctgacg
    20701 aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt gaaaccagaa
    20761 catcagggac tggcgacgga agtgatgtgc cggatgaaca tcgcaggcgc gtcctttgcc
    20821 aacatcgaag gcgtaaaagc gatgaccgac gttacgggct ttggtctgct gggccacttg
    20881 agcgaaatgt gtcagggggc tggtgtgcat gcacgcgtcg actatgaagc gatcccgaaa
    20941 ctccccggtg ttgaagagta cattaagttg ggcgcagtac ctggcggcac tgaacgtaac
    21001 tttgccagct acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga tctgctgtgc
    21061 gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc cggaagcaga aaatgaggtt
    21121 aaaactacag ccgccgagtt tggcattgaa ctgacggcga ttggcgaact ggtgccagcg
    21181 cgcggcggtc gtgccatggt tgagatccgt taatttaatg cggttgttta ttgccgaaaa
    21241 accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa ccgcaccgga aaggcgatgg
    21301 ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt atcggtcacc tgcttgagca
    21361 ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg atttgccgat
    21421 tgtcccggaa aagtggcaat tacagccacg accctccgtg accaaacaac ttaacgtcat
    21481 caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc ggggacccgg atcgtgaagg
    21541 gcaattgctg gtggatgaag tgctggacta tctgcaactg gcaccggaaa agcgccagca
    21601 ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg gttgagcggg cgatcgaccg
    21661 tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct gcgctggcgc gagcgcgtgc
    21721 cgactggctg tacggcatca atatgacccg tgcgtatacc attctcggtc gcaatgccgg
    21781 ttatcagggc gtactttccg tgggacgcgt gcagacaccc gtgctcgggc tggtggtgcg
    21841 ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc tttgaagtca aagcacatat
    21901 cgtgacacct gccgatgagc ggtttaccgc tatctggcaa ccgagcgaag cgtgtgaacc
    21961 gtaccaggat gaagaagggc gcttgttaca tcgtccactg gcggagcatg tggttaaccg
    22021 cattagtggt caaccggcta ttgtcaccag ctataacgat aaacgggaat cagaatccgc
    22081 gccgctgccg ttttcgcttt cggcgttgca gattgaagcg gcaaaacgct tcggtctgag
    22141 cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa acgcacaagc taatcactta
    22201 tccgcgttcc gattgtcgct atttgccaga agaacatttt gccggacgcc acgcggtgat
    22261 gaatgccatc agcgttcatg caccggatct gttgccgcag ccagtggtag atccagatat
    22321 acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac cacgccatca tcccgaccgc
    22381 acggagttct gcgatcaacc tgacggagaa cgaagcgaag gtctataacc tgattgcccg
    22441 tcaatacctg atgcagttct gcccggatgc ggtgttccgc aagtgtgtta tcgaactgga
    22501 cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag gctggcgcac
    22561 gctgttaggc agcaaagagc gcgatgaaga aaacgacggc acgccattgc ctgtggtggc
    22621 gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta gagcggcaaa cccagccgcc
    22681 gcgccatttt accgatgcaa cactgctttc ggcgatgacc gggatcgcgc gctttgtgca
    22741 ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt ctggggacag aagcaacccg
    22801 tgccgggatt attgaactgc tgttcaagcg tggttttctg accaaaaaag ggcgctatat
    22861 ccactccacc gacgccggaa aagcgttatt ccattcgctg ccagaaatgg cgacgcgacc
    22921 ggacatgacc gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc agtgtcgcta
    22981 tcaggacttt atgcagccgc tggtggggac gctatatcag cttattgatc aagccaaacg
    23041 tacgccggtg cggcagtttc gcggcattgt ggctccgggc agtggtggca gtgctgataa
    23101 gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt ccgccagcag atgaagccgg
    23161 aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta ttcgagtcat attctgaaat
    23221 atccagcgga tcaagaaaat tcgttggata tttttttgca tggataaaat tatcgcctct
    23281 aaagtatgta ataacaggga atgtgatgaa aaaagtttta ttgcaaaacc atcctgggag
    23341 cgagaagtat tcttttaatg gctggggaat atttaatagt aattttgaac gggtgattaa
    23401 agaaaataag gccatgctgc tttgtaagtg ggggttttat ttaacatgtg ttgtcgctgt
    23461 aatgtttgta ttcgcagcga taacatccaa cggtttgaat gaaagaggcc tgattaccgc
    23521 gggatgctct tttctttatc tattaattat gatggggctt attgttcggg ccggttttaa
    23581 agcaaaaaaa gaacaactgc attattatca ggctaaaggt attgagccgc tcagtatcga
    23641 gaagttacag gcgctacaat tgatcgcacc ttatcgattc tatcataagc aatggtctga
    23701 aacgctagag ttctggccgc gaaagcctga acctggcaaa gataccttcc aatatcatgt
    23761 gcttcctttt gactcgatcg atatcatcag taaaagacgc gagtctttag agaatcaatg
    23821 gggtatcgaa gatagcgaaa gttattgtgc cttaatggag cattttcttt ctggcgacca
    23881 tggagccaat acctttaaag caaacatgga ggaagcccca gagcaggtta tcgccttgtt
    23941 gaataaattt gctgtttttc cctcagacta tatctctgat tgcgctaata atcgctccgg
    24001 taaatcctcg gcgaagctaa tatgggcggc ggaattatca tggatgatct cgatatcaag
    24061 cacagctttt caaaacggga caattgaaga agaactggcc tggcattata taatgcttgc
    24121 ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa atagtctaat
    24181 gggatttctt tactggcata tctgctgcta tcgcagaaag ttaacggatg ctgaacttga
    24241 agcgtgttat cgttacgaca agcagttttg ggagcactac agtaagaaat gccgttggcc
    24301 cataagaaat gttccctggg gagcatcatc cgttaaatac tcataaacgc ctgaaatttt
    24361 gccgggggcg ctgcgcttga ccggcctaca ttagagcgta actgcaatga attgtgccca
    24421 tctgtaggcc tgataagcgt agcgccatca ggcatttaca acttaaatca caccctgcgc
    24481 cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt
    24541 ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca tgatgtgatg
    24601 caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt gtgccatttc
    24661 caggcccgac gttgctacac cgccagcatt agccgcttta cccggtgcaa acagtacgcc
    24721 tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatttgccc cttcagcgac
    24781 ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt cgttctgggt
    24841 ggcgcaaggc agggcgatat caaccggtac agaccacggc tgttggcctt cgagatagac
    24901 cagaccaaat tctttggcgt aatctgccac tcgaccatcg cggctggctt tgatttcgat
    24961 aagacgtgcc agtttctctt tcgtgaatcc gctttcatca actacagtgc cgctggagtc
    25021 tgacgcagtg atcacacgag caccaaattc catcgctttt tcgatagcgt actgggcgac
    25081 gttgccggag ccagaaacgg aaacgctcat cccttcaaaa cccataccgt ggcgttttaa
    25141 cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa taagactacc
    25201 gccaaatgaa aggcccttac cggtgaagac gcaggcggta ttgttggaga gctttttcat
    25261 catccccgcc ataaagccga cttcacgacc accaacgccg atatcacctg ccggaacgtc
    25321 ggtatccgcg cccaggtggc gatacagttc agtcatcagc gtctggcaaa aacgcatcac
    25381 tttaccttcg ctttttcctt tcggatcgaa atcgctgccg cctttaccac cgcccatcgg
    25441 cagggtagtc agggcatttt tgaaggtttg ttcaaagccg aggaatttga gaatggaaag
    25501 gttaactgac ggatggaagc gcataccgcc tttgtacggg ccgatggcag agctgaactg
    25561 cacacgccat gcacggttga cctgtatctg gttgcgatca tcaacccata ccacgcgaaa
    25621 ctggatcacg cgctccggtt caaccagacg ctccagtaat gacatctggc gatattttgg
    25681 attttgttca agaaaaggcc agagtgtggt cattacttca cgaacggctt gcgcgaactc
    25741 ggtttgattc gggtcgcgct tttggacatg gttgaggaat gactccagag aatatgtctg
    25801 atccatagat ataaaaacct tatatattaa tacgattgtg cttatgttgt ttatgtcatg
    25861 tgcttttgca gttttcgact ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac
    25921 ccaggaaagc aaattaataa cgagagtaat ctcataacca aatgcgataa ctcagacgct
    25981 aaagttttgc cacccggcta ccgttatcag tagcagagac aataacgtta aggagttccc
    26041 tgtgagtcga gcattgttcg ccgtagtatt agcatttcca ttaattgcac tggctaaccc
    26101 gcattatcgt ccggacgtgg aggtaaacgt accgccagaa gtgttcagtt caggcgggca
    26161 gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat caaaattatt ctgaaggggc
    26221 ggtaattaaa gccgaaggaa tactgctgca atgccagcgt gatgacaaaa cccttagcac
    26281 taatccgctg ctctggcggc gcgtaaagcc ataaacgcct ctaataatgg aatgtcagca
    26341 ggggccagcg gatattgcag cgcctcttca ggtgagcacc agaccagcgc ctgatgttca
    26401 tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg catgaagatg gataatccgg
    26461 cccgaaactt ctcgctgatg gctggcaaca tactcaccca cagttgcttc gatgtccaat
    26521 tcttcgttta actcacgcac cagcacctgc tgctggcttt catccagctc gactttacct
    26581 ccggcaaact cccataatcc cgcctgatcg ctctgggcgg ggcgttgcgc gagtaaaatt
    26641 ttgccatcac gttcaatgat ggcggcaaca acttcaatca ttttcataaa ttacacacgg
    26701 gcagataagc gaaaaatgcc ataatatccg cactgttgct caatcagaaa ggctttatat
    26761 ttaaggagtg cctgtgctag accgccatct tcatccccgg attaaaccgt tgttgcatca
    26821 gtgcgtgcgg gttcttgata aaccgggcat tacgccagat ggtttaacat tagtgggatt
    26881 tgccatcggc gtgctggctc tgccgtttct ggcgctgggc tggtatctgg cggcgctagt
    26941 cgtcattttg ttgaacaggc tgcttgatgg tctggacggt gcgctggcgc ggcgcagagg
    27001 gcttaccgac gcgggcggct ttcttgatat ttctctcgat tttctctttt acgcgctggt
    27061 gccgtttggc tttattcttg ctgcaccgga gcaaaatgcg ctggcaggcg gctggttgtt
    27121 gtttgcgttt atcggcacgg gcagcagttt tctcgccttt gctgcactgg cagcaaaaca
    27181 tcagattgat aaccccggtt atgcacataa gtcgttttac tatctggggg gattaaccga
    27241 aggcacagaa acgatcttac tgtttgtgct gggatgttta ttccctgcat ggtttgcgtg
    27301 gtttgcgtgg atcttcggtg cgttgtgctg gatgacgacc tttacgcggg tgtggagtgg
    27361 gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa tgttatttgc tactgcccgg
    27421 gccgcgttca ccggttgcaa ccggattttt tggatcgctg ctccattcgt accagccgcc
    27481 gtcatacacg gaaacattct tccaacccat ggcgcgtgcg tacataaagg tttcggacgc
    27541 gcgccagccg gtgccgcagt agaatgaaac ttgctgatct ggtttgatat tccatgcttt
    27601 ccacatagcg gtaatatcat cggcgctacg catggtgcca tccgggttat agaaatcttc
    27661 catatgcgtc gagtcgctac cagcgtgtcc ccaacgtgct ccggctattt caccttttgg
    27721 tttaatatag ctgtaaccgc tggtcgtacc gataaattct ggccacgaac gaatgctcac
    27781 cagcgatgca tcctggcgat gcagcagtcc acgggcctgt tccatatcga gcatcaactg
    27841 cggttgtgcc gggatcttca cgccgaaatc cggttccgct ttcactttcg gtggcgttcc
    27901 tcgctcaaca ggcagtcccg cgtcggacca ggtttgccag ccgccatcca gcagacgcac
    27961 atctttcacg cctgcgtaga gcataatttg cgccacacgc gctccagcgt atacgtcacg
    28021 cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt tttgccagca tcgcttttag
    28081 ttgttcatca gaaactttgt tccacagcgg ttcgctttcc acttcgttgg tatcgatgta
    28141 gtcagcgcca ggaatatggc tgataaggta aagcttagga gcgccctagg ccgcttcaat
    28201 gactttccag tcaccgacag gtttcgccgt aacctcttta ccttgttgca ggtcatgcag
    28261 ccattgcgga taaaccagct gctcaaagtg cggcagtttt tgtagacggg aaggttcgct
    28321 tagcgcgtca ctcagaatgg agatatgcgt aaaacctgct ttttgcagtc gcgttttgac
    28381 ggcgtcgaca tctttgccat taccgtacag cgccaccggg gcatcggctt tcaggttatg
    28441 ttgcttgatc cacgcgttga gctgttcggt gctcattttg tcgagccagc tagcagagag
    28501 gtttaaggcg gcaggttcat gaccagaagg gccatttaag gtttgtggcc agccgttata
    28561 aaacgcgctg gggcgagtat ctatcgcttt gccattttgt tgctgaagtt ggtcaagtgt
    28621 aagaggcttc gccagttcag cggcccacga agaagcgcaa gccagcccta aagccattgc
    28681 cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc gacattgtag ttttgagtgc
    28741 gccattctga actgacgcat tgagaaaaac tttgcgttat cgcagtttgt tgtaattttc
    28801 tgaccactgc gccatatcca gaacagaact atcagcagga acatcctgga gatcgtgcgt
    28861 tacctgaacg acggggatcg ccagggcgcg aacttcgctg aacacccact ggcgaaaatt
    28921 atcgcgcaga gccacatcaa gacggctgaa tggctcatcc aggagtaacg cttttggctg
    28981 ggcgagaagg gcgcgtagta gagcaacgcg cgctcgctga ccgccagaca aagtggcagg
    29041 atcctgatgg aaagctccgt caaggcctga acgctcaagt gcatcattca cggcattacg
    29101 tcgggcattc cccttaagtg tcgccggtag cgccagcagt aaattttgcc cgacgctgaa
    29161 ctggtcaaat aacagtgcat cctgaaaaag aatgccaatc tgacgctgtg cggtgggtag
    29221 catgtcaatc cgttgctcat tgagccatag ctcacctgta caagaaaact gtccggccag
    29281 tgcaccaatc atccatgaaa acagagtgga ttttccacag ccagacggcc ccattaacgt
    29341 gacgatgtca cctttatcaa ccgtaaagtt aacgtttgtc agcaagcggc tttctggtaa
    29401 acgtagcgaa acatttttca cgcagagcat tagcggattc cttgtctgac ataacctacc
    29461 cattttgcga ctaacgcggt cagggcaaaa ataataagcg gtaacaacag ttgccataac
    29521 gcctgggcgg cgagaatacc gttgctgccg ccgctgctta atgccaccgc ctcagtggtg
    29581 agcgtcggaa aacgccccgc gcccagccac agcgtcggca tatactgcgc aatactgact
    29641 gaaaatccca ccgcgaaggc agtcagcaca gggcgcaaca tgagtgggca tttcacgtaa
    29701 aagaagattt tggcccgcga ccagcccagt gtttgcgcaa tcaaaattaa ccgtgaatca
    29761 atgcgctgcc aggcgggttg caggataaac agcatccacg gcattaccca cagcagatgc
    29821 ccccagacca ccgccgtcca gcttccatcc aggttcagcc atagcgccag cgtgtactgg
    29881 cccgccacca gtggcagagc gggcaataga atgggcaacc atagccacaa ctggcgacgc
    29941 tgtgcccccc attccagcca cagcagtaac aggagcaaag cgatgaatgt tgctaccagc
    30001 cccattgtca gactgttgat gagtgcttca ctattgatcg tcgactgatc cgcgaggatc
    30061 gccagcagaa ccacacacag cacaccggtt aagggtaaaa aactcgccag cgtagtgcct
    30121 ggtaataaag gtgtggcagg cttgcgaatg ccatctacgc ggggaatagt gcgccgccag
    30181 ctgcgccaaa acgtaggcgg cgagtaacag catcagcagc agactggcga gtgcgccttt
    30241 cgtttgttga tccgcgtcgc cctgagttaa ccactgccag ctaattactg ccagcgtcgg
    30301 tggattacct ggcccaagaa taatcgccac atccacgacc gaaagcgacc aggcaacaat
    30361 cgccagcatc gccattgcca gcgaaggcgc gacggagggc aacagcagcc agttcaggca
    30421 ttgccagcgg ctgtagccca gcgaatccag cacaatgacc tgctgcaaca gccgtttttc
    30481 gctcaatact gccactaaga tccacagcag aaatgcgctt tctttcactg caagggtgag
    30541 gcccagaccg atccaaatcg gtccattggc ggagtgaaat acgggaaata gtcatatagc
    30601 agccctccgt cagcaaagag cagcagggcg ctggtggcaa aagccacatg gggaatggcg
    30661 agcagccacg gcagacgggc gcacatacgc tgccatttcg gcccaggcca tagcgccaca
    30721 atcaccagca gggcaatcaa caatgccccg accgccgcga tggttgtcga caccagcgtt
    30781 gccagtaatg cctgcggtaa ctgtggatcg gcaaataacg cctgccagcg tgtcaacgac
    30841 aacgcaggcg atatcagcgt gagagctgcc gggatcagcg gtgcataaat caccgccacc
    30901 atcgcccaca gcaaaaagat taatgcgtac cgtaacggcg taaccattct tgttccagtg
    30961 cattcaccca tcctgcgtgc ggttcagcca gtaccggcgg cagatcctgc ggcattcttg
    31021 attgcaatat ttcgcgctgc ccgtcaggca gtttttgcgg atcgagaaca gaaggatcgc
    31081 cccagacaac gggatctgct ttacgcagtt gcgcatcggg tgagagcagg aaattggcaa
    31141 ctaccttcgc cgcagcactg gcattcgcgt tggctggagt ggtgacgaaa tgcacgttgc
    31201 caatcatccc ctcgcgaaag ccaaaactgt aactgcttgc cggcaaatcg ccgctggcga
    31261 ttttttgctg cgcatgtgcg gggttaaagg tcagcgacag gcgcaatgtg ccggctttca
    31321 gcagagcatc catccgcgcg ggcgaaggcg ggaaatcctt tccttcgcgc cacaaatacg
    31381 gatgcagtgc atcaagatat tgctacaagg gcgcagtgac acgggcgaaa gtcgcatcgt
    31441 ccggcgcttc ttttaatgcg gcgggatctg gcgtcagcat aatcagcaac tgttcaagaa
    31501 acgccgtgcc ggtaaagtcc ggtgggcgcg gataagtaac cgtgccggga ttggctttag
    31561 caaactccag taaggcttgc ggcgtttgtg gtggctgtgg cgtaacatcg cggcgggcga
    31621 taaacgtcag ttgtgcgccg ccccagggcg attccgcacc ttgtgtcggc actgaaaaat
    31681 ctccccgcac cggcagctgt gtgtcgacat agcgccagtt gggcagagtc tccgcccagc
    31741 ccgtttgtaa taaattggcc tcttt
//
LOCUS       DBKELA010000040        31470 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.040, whole genome shotgun sequence.
ACCESSION   DBKELA010000040 DBKELA010000000
VERSION     DBKELA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.040"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1265)
                     /gene="exuT"
                     /locus_tag="ACUQR0_001833"
     CDS             complement(<1..1265)
                     /gene="exuT"
                     /locus_tag="ACUQR0_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HHV9108906.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVA"
     gene            1749..3161
                     /gene="uxaC"
                     /locus_tag="ACUQR0_001834"
     CDS             1749..3161
                     /gene="uxaC"
                     /locus_tag="ACUQR0_001834"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HHV9108907.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3176..4663
                     /gene="uxaA"
                     /locus_tag="ACUQR0_001835"
     CDS             3176..4663
                     /gene="uxaA"
                     /locus_tag="ACUQR0_001835"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HHV9108908.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            4746..5297
                     /locus_tag="ACUQR0_001836"
     CDS             4746..5297
                     /locus_tag="ACUQR0_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HHV9108909.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(5302..6546)
                     /gene="sstT"
                     /locus_tag="ACUQR0_001837"
     CDS             complement(5302..6546)
                     /gene="sstT"
                     /locus_tag="ACUQR0_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HHV9108910.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7142..8107)
                     /gene="alx"
                     /locus_tag="ACUQR0_001838"
     CDS             complement(7142..8107)
                     /gene="alx"
                     /locus_tag="ACUQR0_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HHV9108911.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(8378..9376)
                     /locus_tag="ACUQR0_001839"
     CDS             complement(8378..9376)
                     /locus_tag="ACUQR0_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHV9108912.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9455..10147)
                     /locus_tag="ACUQR0_001840"
     CDS             complement(9455..10147)
                     /locus_tag="ACUQR0_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SanA/YdcF family protein"
                     /protein_id="HHV9108913.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(10220..10723)
                     /locus_tag="ACUQR0_001841"
     CDS             complement(10220..10723)
                     /locus_tag="ACUQR0_001841"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HHV9108914.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            10808..11944
                     /gene="rlmG"
                     /locus_tag="ACUQR0_001842"
     CDS             10808..11944
                     /gene="rlmG"
                     /locus_tag="ACUQR0_001842"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HHV9108915.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            12229..12543
                     /gene="higB"
                     /locus_tag="ACUQR0_001843"
     CDS             12229..12543
                     /gene="higB"
                     /locus_tag="ACUQR0_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HHV9108916.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            12540..12956
                     /gene="higA"
                     /locus_tag="ACUQR0_001844"
     CDS             12540..12956
                     /gene="higA"
                     /locus_tag="ACUQR0_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HHV9108917.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13001..15019)
                     /gene="fadH"
                     /locus_tag="ACUQR0_001845"
     CDS             complement(13001..15019)
                     /gene="fadH"
                     /locus_tag="ACUQR0_001845"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HHV9108918.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(15245..17596)
                     /gene="ygjK"
                     /locus_tag="ACUQR0_001846"
     CDS             complement(15245..17596)
                     /gene="ygjK"
                     /locus_tag="ACUQR0_001846"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HHV9108919.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(17613..18684)
                     /gene="ygjJ"
                     /locus_tag="ACUQR0_001847"
                     /pseudo
     CDS             complement(17613..18684)
                     /gene="ygjJ"
                     /locus_tag="ACUQR0_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(18818..20251)
                     /locus_tag="ACUQR0_001848"
     CDS             complement(18818..20251)
                     /locus_tag="ACUQR0_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9108920.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(20314..20763)
                     /locus_tag="ACUQR0_001849"
     CDS             complement(20314..20763)
                     /locus_tag="ACUQR0_001849"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HHV9108921.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(20760..23837)
                     /gene="ebgA"
                     /locus_tag="ACUQR0_001850"
     CDS             complement(20760..23837)
                     /gene="ebgA"
                     /locus_tag="ACUQR0_001850"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HHV9108922.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24020..25003)
                     /gene="ebgR"
                     /locus_tag="ACUQR0_001851"
     CDS             complement(24020..25003)
                     /gene="ebgR"
                     /locus_tag="ACUQR0_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HHV9108923.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            25221..25552
                     /locus_tag="ACUQR0_001852"
                     /pseudo
     CDS             25221..25552
                     /locus_tag="ACUQR0_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(25594..26973)
                     /gene="ygjG"
                     /locus_tag="ACUQR0_001853"
     CDS             complement(25594..26973)
                     /gene="ygjG"
                     /locus_tag="ACUQR0_001853"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HHV9108924.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            27391..28911
                     /gene="aer"
                     /locus_tag="ACUQR0_001854"
                     /pseudo
     CDS             27391..28911
                     /gene="aer"
                     /locus_tag="ACUQR0_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(29065..29688)
                     /gene="nfeR"
                     /locus_tag="ACUQR0_001855"
                     /gene_synonym="yqjI"
     CDS             complement(29065..29688)
                     /gene="nfeR"
                     /locus_tag="ACUQR0_001855"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HHV9108925.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            29965..30710
                     /gene="nfeF"
                     /locus_tag="ACUQR0_001856"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             29965..30710
                     /gene="nfeF"
                     /locus_tag="ACUQR0_001856"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(30764..30839)
                     /locus_tag="ACUQR0_001857"
     tRNA            complement(30764..30839)
                     /locus_tag="ACUQR0_001857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30804..30806),aa:Ile,seq:cat)
     gene            30964..31470
                     /gene="mug"
                     /locus_tag="ACUQR0_001858"
                     /pseudo
     CDS             30964..31470
                     /gene="mug"
                     /locus_tag="ACUQR0_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         7588 a         8226 c         8295 g         7361 t
ORIGIN      
        1 tgcgccacct caatcgccgg tttgttctgc aatacggtcc agataaccag cgcacccagc
       61 aggtcaaata ctgccagaac tgcgaacagc gggctgaagc cgatggtgtc agccagtgca
      121 ccgacaacca gcgcaaacag cgtacttgcc agccatgcgg acatcccggt taaaccgttc
      181 gccgtcgcca cttcgttacg accgaacacg tcagaagaga gcgtaatcag cgcgccagac
      241 agtgcctggt gggcaaaacc accgatacac agcagcataa ttgcgacata cgggttggtg
      301 aacagaccga tcatacccgg gccaatcatc agcacagcac ccagcgttac gaccatctta
      361 cgggaaacga tcaggttcac accaaaccaa cgctggaaca gcggcggcag gtaaccaccg
      421 aggatacaac cgaggtcagc aaacagcatc ggcatccagg cgaacatcgc gatctctttc
      481 aggttaaagc cgtaaacttt aaacatgaac agcgggatcc acgcgttaaa agtaccccag
      541 gccggttctg caaggaaacg cggcagtgcg ataccccaga actgacggtt acgcaggatc
      601 tgaccaacgg acattttctt cgccgtgctc acctggtgct gggcttcctg accattaata
      661 atatagtcgc gttcttcatc ggtcagatgc ttctggtcgc gcggatgttt atagaaaatc
      721 agccatgcca tcgcccagat aaagctcaat gcaccggaga tgataaatgc catctgccag
      781 ctgtgcatca cgattgccca taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa
      841 cctacgttaa agtagcctac tgcgatggaa cgctctttcg ccgggaacca ttcggagctg
      901 gctttcagac ccgccgggat catcgctgct tccgcggcac cgaccgcacc acgagcaaca
      961 gctaggccac cccagctacc tgccagcgcg gttgcaccac agaacacggc ccacagcaca
     1021 gcaaacattg cataaccgat tttcgtaccc agcacatcca gtacataacc tgctaccggt
     1081 tgcatgaccg tataagcagc agaataggct gcgatgatat aggaatactg ttgggtggag
     1141 atgtttaact cttccatcag agttggcgca gctgccgcca cagtgttacg cgtcaggtaa
     1201 ccaagcacgg tgccgagcgt caccagtgcg atcatatacc aacgtaaccc tttaatttta
     1261 cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag cgcggccatc atcccgccag
     1321 aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa aaccggcacg ccccgaacgt
     1381 tgccatgaaa gtttcgtgct aattcggctt ccgtaccggt taacccgatt catctaacaa
     1441 atgaatgaat cggcaatttg cattccgtcg acttatgatt tgcgacggca gaaagataac
     1501 ttgtcataca accttaaaag gtgagagcca tcacaaatgt gcgaatattt gtagggacat
     1561 tacctgacga cagcgaggcc agtactggcg cggcctgcag cgagatttac cactttgaga
     1621 gtaatttttt taactacgtt tattgatcta actcaagaaa atatcttcgg actctggaaa
     1681 ttggtgtgat aactttgtca gcatcgcacc ataagcaagc tagctcactc gttgagagga
     1741 agacgaaaat gactccgttt atgactgaag atttcctgtt agataccgaa tttgcccgcc
     1801 gtctgtatca cgactacgca aaagaccagc cgattttcga ttaccattgc catttgccgc
     1861 cgcagcagat tgcggaagac tatcgtttta aaaacctgta tgacatctgg ttgaagggcg
     1921 atcactacaa atggcgcgct atgcgtacca acggtgtggc tgagcgtctg tgtactggtg
     1981 atgcgtctga ccgtgaaaaa tttgacgcct gggcggcgac tgttccgcac actatcggca
     2041 acccgttata ccactggacg cacctcgaac tgcgtcgtcc gttcggtatc actggcaaat
     2101 tgctttctcc gtcaactgcc gatgaaatct ggaacgaatg taacgaactg ctggcgcagg
     2161 ataacttctc cgcgcgcggc atcatgcagc agatgaacgt gaaaatggtc ggcaccaccg
     2221 atgacccgat cgattctctg gagcatcacg cagagatcgc caaagacggc tctttcacca
     2281 tcaaagtgct gccgagctgg cgtccggaca aagccttcaa tatcgaacag gcgaccttta
     2341 acgactacat ggcgaagctg ggcgaagttt ccgataccga cattcgccgc tttgctgacc
     2401 tgcaaactgc cttgaccaaa cgtctggatc acttcgccgc tcacggctgt aaagtgtctg
     2461 accatgcgct ggacgtggtg atgtttgctg aagcgaacga agcggaactg gacagcattc
     2521 tggcgcgccg tctggctggc gaaaccctga gcgagcacga agtggcacag ttcaaaactg
     2581 cggtactggt gttccttggt gccgaatatg cacgtcgcgg ctgggtacag cagtaccaca
     2641 ttggcgcgct gcgtaataac aacctgcgtc agttcaaact gctggggccg gatgtaggct
     2701 ttgactccat caacgaccgt ccgatggcgg aagagttgtc taagctgctg agcaagcaga
     2761 acgaagaaaa cctgctgccg aaaaccattc tctactgcct gaacccgcgc gataacgaag
     2821 tgctgggcac catgatcggt aacttccagg gcgaaggtat gccgggcaaa atgcagttcg
     2881 gttccggctg gtggtttaac gatcagaaag acggtatgga acgtcagatg acccaactgg
     2941 cgcagctcgg tctgctgagc cgctttgtcg gtatgctgac tgacagccgt agcttcctgt
     3001 catacacccg tcacgaatac ttccgccgca ttctgtgcca gatgatcggt cgctgggtgg
     3061 aagcgggcga agcaccggcg gacatcaacc tgctgggcga gatggtgaaa aatatttgct
     3121 ttaacaatgc gcgtgactac ttcgccattg aactgaacta aggtctgggg ttgatatgca
     3181 atacatcaag atccatgcgc tggataacgt cgcggtcgct ttagcagatt tggctgaagg
     3241 cacagaagtc agtgtcgata accagactgt tacgctgcgc caggatgttg ctcgtggaca
     3301 taaatttgcg ttaacggata tcgcaaaagg ggccaacgtc atcaaatatg gcctgccgat
     3361 tggttatgca ttggcggata ttgcggcggg ggaacacgtc cacgcccaca atacgcgcac
     3421 gaatctgagc gatctggatc agtatcgcta tcaacctgat tttcaggatc tgcctgcgca
     3481 agcggcagat cctgaagtgc agatctatcg tcgcgctaac ggcgatgtcg gggtgcgtaa
     3541 cgagctgtgg atcctgccaa ccgtgggctg tgtcaacggc atcgcgcggc aaatccagaa
     3601 ccgtttcctg aaagagacca acaacgccga aggtaccgac ggcgtgttcc tcttcagcca
     3661 cacctatggc tgctcacaac tgggcgacga tcacatcaac acccgcacca tgctgcaaaa
     3721 catggtgcgc cacccgaacg cgggcgcagt gctggtgatt ggtctgggct gtgaaaacaa
     3781 ccaggttgcc gcattccgtg aaacgttggg cgatatcgat cctgaacgcg ttcatttcat
     3841 gatctgccaa cagcaggatg atgagatcga agccgggatc gagcatttgc atcagctgta
     3901 taacgtgatg cgcaacgata aacgcgagcc aggcaaactc agcgaactga agtttggtct
     3961 ggagtgcggt ggttctgacg gtctttctgg tattactgct aacccgatgc tggggcgttt
     4021 ctctgactac gtgattgcta acggcggtac taccgtactg accgaagtgc cggagatgtt
     4081 tggcgcagag cagttgctga tggaccattg ccgcgacgaa gcaacgtttg aaaaactggt
     4141 caccatggtc aacgacttca aacagtactt tattgcccat gatcagccga tctacgaaaa
     4201 cccatcaccg gggaacaaag cgggcggtat caccacgctg gaagacaaat cacttggctg
     4261 tacccagaaa gcgggttcca gtgtcgtggt tgacgtgctg cgttacggcg agcgtctgaa
     4321 aacgccaggg ctgaacttgt taagtgcgcc gggtaacgat gccgtagcga ccagcgccct
     4381 ggcgggtgcg ggctgccata tggtgctgtt cagtactggt cgcggcacgc catacggtgg
     4441 atttgtgccg acggtgaaaa tcgccaccaa cagtgaactg gcggcgaaga aaaaacactg
     4501 gatcgacttt gacgcgggtc agctgatcca cggtaaagcg atgccgcagt tgctggaaga
     4561 atttatcgac accatcgttg agtttgccaa cggtaagcaa acctgcaacg agcgtaacga
     4621 cttccgcgaa ctggcgatct tcaaaagcgg cgtaacgcta taaagtcgaa atgagttgtt
     4681 aataaaaaac ggcgtttcat aatgtgaagc gccgtttgct tttccatacc tgctaaggat
     4741 ttatcatgac cgcgtattgg ctggcccagg gcgtgggtgt catcgccttt ctgattggta
     4801 tcacaacatt tttcaatcgt gacgaacgtc gcttcaaaaa gcagctttcg gtctatagcg
     4861 ccgttattgg cgtacatttt tttcttctgg gcacctatcc cgctggtgcc agtgccatcc
     4921 ttaatgccat tcgtacattg attaccttac gcacgcgcag cttatgggta atggcgattt
     4981 ttattgtgct gactggcgga attggcctcg cgaagttcca tcatcctgtc gaactattgc
     5041 cggttatcgg tacgattgtc agtacctggg cgctgttccg ctgtaaaggg ctcaccatgc
     5101 gctgcgtgat gtggttttca acgtgttgct gggtgattca caacttctgg gcggggtcga
     5161 taggcggcac gatgattgag ggtagttttc tgcttatgaa tggcctgaat atcattcgtt
     5221 tctggcggat gcagaaaagg ggaattgatc cgtttaaagt agagaaaacc cctcccgcca
     5281 tagacgaaag gggttaaaca attaattacg cagggcgcta tttgccagac gatcgtcttc
     5341 cgcctggcaa gctgccgcag tgaacagcac gtcagttgaa gagttcagcg cggtttcgca
     5401 agagtcctgc aatacgccga taataaagcc gacggcaacc acctgcatgg cgatatcgtt
     5461 cgaaataccg aacatattac aggccagtgg gatcagcagc aaagaacccc ccgccacgcc
     5521 ggatgcgcca caggcacaca gagaagccac tacgctcaac agcagcgctg tgggcagatc
     5581 gaccggaata cccagcgtat taaccgcagc cagcgtcaac acggtaatgg tgattgctgc
     5641 gcccgccata ttgatggtgg ctcccagcgg aatagaaact gaataggtat cgcgatccag
     5701 attcagcttt tcacacagcg ccatattcac cggaatgttc gctgcagagc tgcgggtgaa
     5761 gaaggcatac acaccgcttt cgcgcaggca cagcagcacc agcgggaacg ggttacgacg
     5821 aattttccac cacaccagca atgggttaac caccagcgcc accagtaaca tacagccaac
     5881 cagcacgacc agcagttgcg cgtagcccca cagtgtggag aaaccggtgg ttgccagggt
     5941 agaagaaacc agcccaaaaa taccgaacgg tgcgaagcga atgaccagtt tcaccataaa
     6001 ggtaacggca ttcgacatat cattgaccag gtttttggtg gtctcgttac cgtgacgcag
     6061 tgcgaagccg aggccgatcg cccacaccag aatcccgatg tagttacctt tcagcagggc
     6121 gtcgatgggg ttggaaacca tgctcattac cagtccgcgc atcacttcga caatgcctga
     6181 cggcggcgaa atatcacccg cgctactgga caagtgcagg gtagaaggga aggcaaagct
     6241 gaagactact gcggccagag ctgcagagaa ggtgcccagc agatagagga acaaaatagg
     6301 acggatattg gttttctgcc cgtgctggtg gttagcaata gatgccatta ccagcatcaa
     6361 caccaggatg ggggcaacag ctttcagtgc gccgacgaac aaagtaccta acagaccaac
     6421 agcttccgcc gcgggttttg agatccatgc cagaagaatc cccagaacaa ggccgaccag
     6481 gatttgtttt accaggctgc catgagccag acgccggaat agccccggtg aacgttgcgt
     6541 agtcattttt cgatcctttc attgtgttgt cgcacatccc tggtgcgttc tgtcgacgca
     6601 tctttcagga tgtaacaaag tgtttgcaag gtcgagtata aggaaagtta cagcttcggg
     6661 aagcgaaaaa tgctggattt taaagagagg tcatgttttg caacttaatg gttgcattta
     6721 tttaacataa acagagggaa ggactggcta ctaaggctga ttagataaca tatgggcaaa
     6781 actatttgga atatgagaac ggttgcgctc ctttaacgtg ttatgccata taaagtcact
     6841 ggcgcaggga gcgcgcaggg ggcggccaat cgccgccgcc ccctgcaccc ccaggctctg
     6901 gcgaacaaaa tcgccgctgc gcggttccct cagcctatcc cttgcggcta ccgggtcggg
     6961 cgcaaggtaa cgtccctgta agatgcgccc tcgccccggc ctccagggaa cgcttcggcg
     7021 attttgacgc caccaacacc cgtgcggtct ttttgtaaag agctataagc cgattacata
     7081 attaatagct cacctttgtc tggaatgagt tatctctagg aatggcttaa tatacagcga
     7141 atcattctcc ccgctgctta tcatgccgat aattcaccca ggcgttgata ataaacgtca
     7201 tcaccagaat gccaaacacc acgcccagcg agacggcgat tggaatatgg tagaagtcga
     7261 caatcagcat cttgataccg ataaacacca gaatcaccgc caggccatat ttgagcatcg
     7321 agaaacgctc tgctacgccc gccagcagga aatacatcgc acgcaggccg aggattgcaa
     7381 acaggtttga ggtcagcaca atgaacgggt cagtggtcac ggcgaagata gccggaatgc
     7441 tatccacggc aaaaatcacg tcgctcaatt ccaccagaat cagtaccagc atcagcggtg
     7501 tggcgtacag caatccattc ttacgcacaa agaaatgctc gttgtcgatg gtgtcggtca
     7561 tgcgcaaatg accgcgtagc cagcgcacca gcggcttgtc gccaatgcct gattcatctt
     7621 catgggcgag cgccatcttc acgccggtaa acagcaggaa ggcaccgaag atatacagga
     7681 tccagtcgaa ctgcgaaatc agccagctgc ctgtgaagat catgatggta cgcagaacaa
     7741 tcgccccgag cacgccatac accagcacgc gacgttgtaa tgccgccgga acggagaaat
     7801 agctgaacaa catcagccag acaaagacgt tatcgaccgc cagcgatttc tcaatcagat
     7861 aacccgtgag aaaggccagg gcctgtggat cggcaacggc gcgaccttcg gtttgcacca
     7921 gataccacca gaaagcggcg ttaaacagta acgacagcgt cacccagacc agcgaccagg
     7981 ccgcagcctg tttcatggtc atggcatgtg ccccacgacg cccctgcaac aacaggtcga
     8041 tagccagcat aatggcgaca acaacagcga atccgcccca tagcaacggc gtgccgacag
     8101 tattcataga agttccttac acataaaaaa acggccaacg tcggaagacg tcagccgctg
     8161 ctttttatgc atagacctcg ccttccggca aggtctcact tacaacaaaa aaggaatacg
     8221 tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg tttttcacac atcgtaatga
     8281 cgatcgaccg gcaatgaagt tactcccctt tgcaggtaac aaagtatgac agaccattca
     8341 gtcagtcaat ggtctgttac atacctttta cactgtttta ctttggcaag tatagtttta
     8401 cgctatctgc cggaaaaatc accccagttt ggcggcggat ctcggtcagc agtttggctg
     8461 tgatgcgact gaccgccagc cctggatgat ccaccagatg ctcatccacc agggtagcga
     8521 acagctctgc ttcgtagagc atggtattaa tatgctgcgg ctgggtgaga tcctgcatct
     8581 ggctgccacg cggcacaaag cagactttct ggcattcaga cagtttttca atcaccagcg
     8641 atcccgcttc gccctgaatc tcgcttccca ggacagaatc actgaccttt gagtgctgca
     8701 aggtgacgct gaaatcaccg taatccatca ccaccacgcc gtgggcgtca acgccgcttg
     8761 ccagcaaact cgccgttgcc tgcacgcttt tcggctcacc aaataacgcc accgccgacg
     8821 ccaggcagta aaagccgata tccataattg aaccgttaga gaatgccgga ttaaaggtgt
     8881 tgggattctc accgtccagg taacgttgat accgcgagga atactggcaa tagttgaaaa
     8941 agactttacg cagttttcct gctttcggca gcgcctggcg caacaaatga aagttcggca
     9001 ggcaggcggt tttaaatgcc tcaaacagca ccacctgatt ttcgcgcgca caggcaatgg
     9061 cggcatccac ttccgccaga ttcgacgcca gcggtttttc gcaaatcaca tgaattttat
     9121 ggctaaggaa aagttgtgtc tgggaaaagt gcagggaatt cgggctggca atatacaccg
     9181 cgtcaatggc atcgctttcc gccatcgctt ccagcgacgt aaacagatgc tcgacagaaa
     9241 aatcattggc gaagtgctga gcctgctcaa ggctgcggga atatacggcg gttaacttgt
     9301 atttaccgct ctcatgggcg gcctcgacga actggcgagt gatccagttc gtaccaatca
     9361 cagcgaaacg tatcataacg cgtggatact ccataaaggg attctgtcgt caatgtagca
     9421 cgcctttttc atcttcgacg tgcgcagata cgcattattc tttcgccgga cagccatgct
     9481 cactaaacgg cccaatcatg accgatggcc cgagatattt cggctggcga tggaggattt
     9541 ttgcgtcaat caccgcgctg acacgagcga gtttctcccg cagttgagta taaaaaccgt
     9601 gcttcatatt gaggtctggc gcgttaaacc cgatagcgtc gatgccgtac tgttttgcca
     9661 gccagatggc gcgttggtta tgaaattctt gcgagataat ggtgatatgg ttttccccaa
     9721 acaccttttt ggcacgcact accgaatcca gcgttgagaa tccggcatag tcgcaaaaga
     9781 tcactttcgc cggtacgcct ttagcgatca aagcctgctg catacctgat gcttcatcgt
     9841 aatttttacg tccgttatcg ccactcacca gtagccattt cacttttccg gcgtggtaca
     9901 acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt acccggcctc gcccccagca
     9961 ataaacccac gttacgcgcc gggacggcgt tgacatcgcc ccaggtcagc tgcttgctgg
    10021 cgttgaccat aacgcgatcg gcgataaaaa tggtcgctcc tgccacaagt aacagtaagc
    10081 aggcaatctt tagagtccgg cgagaaaaac aaatacggag aagaaggcgg gcaaatgcgc
    10141 gcagcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaag
    10201 ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc gttagccaca
    10261 gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac aattgataga
    10321 acgctttgtt atgctctttc tccttcaggt gcgccagctc gtgcaccacg atcatgcgca
    10381 aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct tttgctttca
    10441 gcttgccgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt tttagcacat
    10501 ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga aactgatttt
    10561 tcagatcctg agtatattgc cagagagctt tatcggtggc gtagtcgtgt gttcccggat
    10621 agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc acctgcgaaa
    10681 gcagctgctc gggataaccc tgaagataag taagattgct cataaccgcc cacgaaaaag
    10741 agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt atcattcagg
    10801 agggccgatg agccacttag acaacggttt ccgttcactg acactacaac gttttccggc
    10861 gacggatgac gttaacccgc tacaggcgtg ggaagcggca gatgaatatt tgctgcaaca
    10921 gttggacgac acagaaatcc ccggcccggt gttgatcctg aatgatgcct ttggtgcgtt
    10981 aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca tcagtgaact
    11041 ggcgacgcgc gagaatttac gcctaaacgg gattgatgag tcgagcgtga agtttctcga
    11101 cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc cgaaaacact
    11161 ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg atacacgtat
    11221 tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt tcgaaaaagt
    11281 gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta attgcacgtt
    11341 caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg aaggtactga
    11401 ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata tcggcgcgcg
    11461 cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc tcggttgcgg
    11521 taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag tggtgtttgt
    11581 cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca acatgccaga
    11641 ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg agcctttccg
    11701 ctttaatgct gtgctctgca accctccgtt tcaccaacaa catgcgctga ccgataacgt
    11761 cgcctgggag atgttccact acgcccgccg ctgcctgaaa atcaacggcg agctgtatat
    11821 cgttgccaac cgccatctgg attacttcca taaactgaag aaaattttcg gcaactgcac
    11881 cactattgcg acgaataata aatttgtggt gctgaaagcg gtgaagctgg gacgtcgtcg
    11941 gtaagattgg gagttacctg ccggatgcgg cgtaaacgcc ttatccggcc tacgttcagc
    12001 acgttacccc taggccggat aagacgcggc aagcgtcgca tccggcaatg tctgctcagt
    12061 tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac attctcttgt
    12121 ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt tgccaatctg
    12181 tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat gcacctgata
    12241 actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac ggagttggtg
    12301 gctctgggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt aaaagcagta
    12361 ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa tgttgggggc
    12421 aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta catacgtgaa
    12481 gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa ggggaaaaaa
    12541 tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgctgtg gcaccttttc
    12601 tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta gatcatctgc
    12661 tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata accgcgtggg
    12721 aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg cctggcggta
    12781 tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat ctgccggaaa
    12841 ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta acgctggaac
    12901 acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt gattaacgcg
    12961 acgtgccgga tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc agcgccagcc
    13021 gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg cagccgccaa
    13081 ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg tttggctctt
    13141 gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg ttgatcacca
    13201 catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt ttcacacccc
    13261 gcgagagcag gatggtgcga tggatccagc cggtggtttt gcctaacccc tgtcccggtt
    13321 tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc tgcattccct
    13381 gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca ttacagaacc
    13441 tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc gtatcaaaac
    13501 caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg tcgcgcagta
    13561 catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga acacgcggca
    13621 cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc gtcacggtgt
    13681 gattgagttt cagcgtcacg cccgtcactt cgatcatccg gcggtagtag cgcagcgttt
    13741 cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg ccgatctcgc
    13801 tgtgcgcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg gcaaacgcca
    13861 gcccagcagg tccagcgccg accaccgcca gatttttttt ctgcacggcg ggaaggattg
    13921 gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact ttgccaacga
    13981 agatctgatc gaggcagccc tggttgcagc caatacaagt gttgatctca tcggctcgcc
    14041 ctgattgcgc ttttgacagc agttccgcat ccgcgagaaa cggtcgcgcc atcgatacta
    14101 tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta atccggttgg
    14161 tggttaccag cggcagcgag acgtggcctt tcagtttgcg cgtgacccag ctaaatgcgc
    14221 cgcgcggcac gggcgtggcg atggtcggaa tccgcgcttc gtgccagcct attccggtgt
    14281 tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ttctaccgtt tcggcaaaag
    14341 tgccgccgcc ttcgaccagg tcgagcatcg acaaccggta aataataatg aagtcgttgc
    14401 cgacgcgttc gcgcaccgta cgcactactt ctacggcaaa tcgcatccgg ttgcggtaat
    14461 cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg ttgatcaaat
    14521 acccttcaga gcccatcact tcgacgccat cgtatccggc ctcccgcgcc agttgcgcgc
    14581 agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg tgaggaacga
    14641 aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg tagctgtagc
    14701 gcccggtatg caaaatttgc agggcgattt tgccgccttc ctgatgtacg gcttcggtaa
    14761 tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct tccatgccaa
    14821 cgcctgttaa atctggtgca atgccgccgc tgacaatcag cgccacgccg tgacgggcgc
    14881 gttcggcata aaacgcagcc agccgttcag caccgtccgg gtattcctcc agcccggtgt
    14941 gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc agcggggcga
    15001 acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat gtagtcggat
    15061 gagttctaat ttagccgtcg atggagaaaa gggaaaaggg gagtgcgggg tttgtgatgg
    15121 gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt gttttatccg
    15181 gcttggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg ccagccgttg
    15241 caaattactg cttacggaaa aaatcgttat acaacatata caaatgagcg gcactccagg
    15301 agaaatttgg tgcgccttgc tgcgcgcctg tcagcgggtt gtaattctcc tgaattgggc
    15361 catcggcggt taatcctttg gcgtgcctga agaacgtatc cgccagcttc agggcatcat
    15421 cacgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc acccatacgc
    15481 gcccgcgcca gtagatatca gcgccaaaag ccggattggt taacgccgcc gttcccagcg
    15541 gaacaaaggt gttgaactct ttaggatcga gcatcacctt caccaccgcg tcggcattgg
    15601 cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta ccgcgctcaa
    15661 caatcggttt gcccgcgcag ccgttcgcca gtggtttatc ttcaatacgt acgtcatagt
    15721 agaactgggt agtcgggtcg aacatacagg tgttgatgta gtccgcgagc tgctgtgcca
    15781 actggcgata gcgtttggcc tcttctggct taccgaggat ggttgccatc tccgccagat
    15841 aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat agcgagtagc
    15901 ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt caccgtccag tcgctacgtt
    15961 tgccgccatt agcgacatat ttatccaact gttctttgtc gataaaacca aagacggcgg
    16021 catcatcacg gcccgattcc cacgacgcag cgacctgtgc cggaatttcc agactgtcgt
    16081 actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc tcttctttat
    16141 cgcctttttt caccgtaaac agcatctcgc cgctctcggt gttgtgggct ttgtcgcggg
    16201 tcgcgccata ttccggcacc ccgttgccgt tatgatcgcg gttacgtaac caccagtcgt
    16261 gataggccac cagtttcggg tacatctctg ccaaccaggc tttatcctgg gtgacgttgt
    16321 atacttccat caccgaccag gcggcaaggc tgggtttggt attacgttcg ttccagttgc
    16381 cgccatcgcc accacgctcg gggctaaggt tccacgctat caggtcgggg acaaagccca
    16441 catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc gcgcggatat
    16501 tctctttggc gatatccgga ttgaaatgcg ccatcgcaaa cgcctgcttc caggtatccc
    16561 acggccaggt ctgattgccg gagaaccagc gtccggtcac cgacggcgtg acggtgttat
    16621 aattcaccgc accgcccggt gagcgccagt taccgttgag cgtttcgatc gctttcaccg
    16681 cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc aggtattctt
    16741 cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata tcgcggatct
    16801 gcatttgctc tttgctaact tcctgagcgg tcagcagatg ggaataggtg gtgtagagcg
    16861 tggtcgaacc gttgatatgc gccttactgg taaagcgatt accgttgatt tcagtctgca
    16921 ccggcaggga tttatgcacc tgatattccg attcgccgga ggtcagcaga tcccaggtgg
    16981 cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt ttgcgctgat
    17041 agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct ttcgcttcca
    17101 gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg atgattttag
    17161 tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc tgcacatctt
    17221 ttgctgtcag tttttgcacc agcgcgccgg gaatactgta tgcctccagc gtgaagttga
    17281 ctttcttgcc gtcttgccag acagtcaggc ggtcgaaatt gctggccata aagttgatgt
    17341 attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg ccgtctggca
    17401 acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg tgatcgtcgt
    17461 aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt ttataattgt
    17521 cggcgttagc ggcatgagcg gaaaaactta tcagcagagc gcaggttact ggcgttaaaa
    17581 tagttttgat tttcatgaga ttacagtcct tattaccagt agaaatattg catcaggaag
    17641 atttggtcgc tgggatcgtt agaggcattt ttcatgtaga agcggctatc aaaagccgtg
    17701 gcgaaacgtc cgccgccata ttcgtaccag ataccaaact gcatgaaaga ggtggtttca
    17761 tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag cggtggagag ataaacgcct
    17821 tttaacgctt catacatggc gctcaccatc atcccgcttt cacttcccgg tacgtccagc
    17881 ccgtcggcac gtccggtatg gtgccaggcg cgagcggcaa agttaggggt aaacaggccc
    17941 gtgagataga gttgtcctgt gccgtcggta atttccagac cattcatcca ggtgatgcct
    18001 tcggcaaggt catactggat gtaaccgttc atccattgcc ggataggtgt atttgtcgtc
    18061 gtagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat gtccggcagg
    18121 tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca gcgcggtcat
    18181 aatgtcgcgg gtattgggaa tgacgtagcc caaatcgggt gtgaagtcgc cccaccattg
    18241 caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc catcattggt
    18301 acgataaccg ccatagaaac ggttaatccc accttcgaaa ccaccccagc tatggtcagg
    18361 cacctaggca ttaccgtcgt ggtcggcctg cacggtccag ccttcgccgt aaatcaggcc
    18421 gaaccaggag ccgtacttca tctccaggcc accttcaata taggtgccgt cgctgtattt
    18481 atgtttattc tcaccctcta aatccatata tccaccaagc cctaattcgc cgtaaaaacg
    18541 aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat gttctgccgg
    18601 acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa gtaatgctct
    18661 gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa agtacagcct
    18721 tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc ctcatccagg
    18781 aagagggaaa atgcctgctc attttttatt agtctgctta atcaacattg ctggctggcg
    18841 tcgcttcaat atggcgctct tcagccgtta atccctttat atatttgctg tatttccacc
    18901 acgcaaagcc gaggaagata acaataccgc cgacgttata aaaaatgatg gtcagaatat
    18961 tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca atcagcattg
    19021 aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc aggtgatcta
    19081 atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc atggacgctg
    19141 cggcggtcat attaataatg gtattcatca gatcctgcac ggtattggaa ccgagcatcg
    19201 ggataatcat cagcgggatg acgatcagaa actggatcca tgctgcgcgc gccggaacgc
    19261 cgttttcgtt aagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
    19321 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacattgcg gtaaaggaca
    19381 ccagaccgac aaagcgattc atcaacgctt ccggcagacc aaaatacgcc gccatgccgt
    19441 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
    19501 tcagcaccga ggagacggaa tacagtacgc cgataaaaat cccggcgagg atgatcactt
    19561 taacgaacga tttcgaaccg cctttgacgt cgttaacgta caccgcgacg gactccgcgc
    19621 cacctgcggc cataaagatc caggtggtaa cgccgaggaa cgcccagttg aagttcggga
    19681 tcatcgcgtc aacggtgatc ggatcggcag gctgtacgcc gccaaccagt gccgtacctg
    19741 ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg gaggtaattg
    19801 gccccagcat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac agcaccatac
    19861 tgataacggt ggtggcaacc ggcgtcataa tatattcgta gccgaggaag gcatacgaag
    19921 cataagcaat aacgcgcggc aacagcgagg tgaaaaagaa cagatttacg aaccagtaag
    19981 tgtaggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccac gcgtagacac
    20041 cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag aagggaataa
    20101 aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaagc tcaatattat
    20161 tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc aagccgaatt
    20221 tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatgt gtagggtagc
    20281 ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat aaccatcttc
    20341 gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc gatacgcttc
    20401 atggatatcg caaataacga tttgcccttc gtggacctct acggtttctc cgcagccttt
    20461 taaatattca cggtcagttt catcacgata atattccact acctgtaatg tctctttcgg
    20521 cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat agcgtcgatg
    20581 cccggtaaat agcgcatcgg tcgcggagtc attttccaca cggtaggtca atgaatcacc
    20641 gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt caacgcagcg
    20701 ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat cgatgatcct
    20761 cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa ctcatacgac
    20821 gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa gccgtagctg
    20881 aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga gttggagccg
    20941 aggccaagta gctggtggtc gaggttcagg gtgatgtcat cactgcgctg cagctcgtta
    21001 cagtgctggg cagcgtggat gttttcctgg gtatagtgcc aggcgctgaa gttaattggg
    21061 cgctgcggaa ccaccagcag accgttgccg tggcggttag tcagtgccgt ccagcggaca
    21121 tgctgacggt tgccgttgtt ctgcgggaag ggatagttct cgaacatggc atcgacggtg
    21181 ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc gcccggtcca
    21241 cgaccgtaat acgccacctg atcgtattcg ccgttaatcc ccatggtgaa accgatgcac
    21301 ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt aacctggcca
    21361 tcggcagcga tgcgccagat gtaggtacag cgcatcccga agtcaaacac cggcggtgca
    21421 ataactgtgc ggctgatgat caacacttca ccatcgctct gttctacggc aaagtcgcgc
    21481 agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta ctcctgcttg
    21541 tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag cagcgactcg
    21601 ccatttacct ggcaggatgt cggtttgcca ctcatttttg agaaggtgat cgcgaagttg
    21661 tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg cgcattattt
    21721 ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata agaggcgata
    21781 gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat gttgaggaac
    21841 gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct gttcggcgca
    21901 acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg cacctctgcg
    21961 tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt caactcgccg
    22021 cgagtcagcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt caggcccgat
    22081 cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt gttgggatag
    22141 tcgccgccga atttatacca gacattgccg tggtcgtcct gtgcctggat cccgtggtcg
    22201 caccactccc agacataatg accctgaatg caatcgtgct tatagaagac gttctggtac
    22261 tccgtcagcc cgcccggtcc gttccccatc gcatgagcat attcacagat gatgcgcggc
    22321 ttcggatgcg ggtattcacc aaactcattc atcagcggca cgcgggtgta catggtggaa
    22381 ataatatcga ccacttcagc atcgcgatct tcttcgtaat gaaccagtcg cgtgtcatcc
    22441 agcgctttcg ccgcatggta catcgcgcgg atgttacagc catagccgga ttcattgccc
    22501 agcgaccaga tgatgatcga cggatggttt ttctgtgcgt ggatatggcg aacaatgcgc
    22561 tcgacgtaga ctttttccca ctgcggatcg tcggtaatac ggctgatatc gccgacatta
    22621 gcaaagccgt gcgattcgac gtcggtttcc gccatcacaa acaggccgta gatatcacac
    22681 agttcgtaaa aacgcggatc gttcgggtag tgagcggtac gcacggagtt gatattgtgc
    22741 tgcttcatca actggagatc tttctcgacg cgatccattc caacggcgcg gcctttgcga
    22801 tgatcgttgt cgtgacggtt gacgccgtgc agcatcacat aacggttatt gatccagaac
    22861 agaccgtcgc gcactttgat atcacggaag ccaacgcgtt gtggcaccac ttccagaacg
    22921 ttgccgttgg cgtctttcag cgtcatgacc agatggtaaa gataagggga ttctgctgac
    22981 cattgctgcg gctgttcgac agtaaaagca aagctggcgc tggtcagttt ttcaattgcc
    23041 aaatgatcaa tggcgctgct gtgcaccacg cgttcgccat caaacagggt atattccagc
    23101 gtcgtgacga caggggaggc ggcgagattt tccagcacca cttcgcagga aagcgtggca
    23161 tcgcaatagg cttcgtcaaa gtcggtacgc acagtgaaat cgttaatatg cgttaggtgt
    23221 tttccgacca gataaacatc gcggaagatc cccgccgacc accacatatc ctggtcttcc
    23281 acgtaggtag agtccgccca ctgcatcacg cgcacacaca acaggttgtc gccggtttta
    23341 accatcgcgc tgatgtcaaa ctctgcggtc aggcgactgc ccttgctgaa acccacatac
    23401 tgaccgttaa cgtagacttc aaaataggtt tcgacgccgt caaatttaat cagcgtctgt
    23461 ttaccctgcc agccgtcgct gagggtgaaa atacgttgat aggcaccggt tgggttatcg
    23521 ctggggacaa acggcacatc gatggggaac ggaaaacctt cgtcggtata ttgcagtttg
    23581 ccgtgacctt ccatttgcca catggcgggg acggtaatat gcccccagtc agccattaac
    23641 tcagaggtga aggcttctgg tacttgcagc ggatggtcaa aaaagtggaa attccactga
    23701 ctgcttaagg gcagaaacag gctgctggtt tcgcgggcaa aggtacgcgc ttgcgcaaca
    23761 gaatcatatg aaaaaaagta cgcacgcggc gcaagtcggt tttcgtgggt gagctgaatg
    23821 ttttcccagc gattcatagg gcctcccggt agagagaaat cggaaccatt catggtgatg
    23881 gtagtttgtg taattgctgg ggggttagta aaatttttac taaaaagcag caggtaaatg
    23941 catttacttt aactggatca cgaaaataaa cggcagcact gtccggcaaa aatgattgcc
    24001 ggacagtgtg taaggggatt taacgggtcg tgccgcgcag ttttaatttg ctgggaacga
    24061 agactagcag cggcagcgcg cgaccatcgc gggctttttc atacaccagg ttaacgccct
    24121 gacttcccat catctcggaa tggatgcgca cggtggagag cggcggaaag gtaaatcgcg
    24181 cggtggggat atcgttaacg ctgataagcg aaatatcttg tgggatgttc aggccacgtt
    24241 catgaattgc ccgcagtacg ccgatagcaa tggaatcgga agcaacaaac agtgccttcg
    24301 gatagtcttc ccgcgccagc atttgttttg ccagttcata acccgacgaa ctggaaaaac
    24361 cgccgcgcca gatatcctct tcgcgtacca cttgtttcag tcggccatat tccgcaaagg
    24421 cgacctcacg aatatccgcc ttgccaggct catcttcacc gccaataaaa ccaatacgat
    24481 taacgccctg gttgatatag aagtcgatga tttctttact gatgcgtgcc agatcgatat
    24541 ccaccgcatc gtaaccgctg ccgggttcgt gaaagtcgat aaaacagata ttgtcggtca
    24601 acgcgctggc agcggcgcgc agggcgggcg tgggtttgcc gacaattaaa ataccggtga
    24661 cgtttttaat gtctggtaat ccgttgtgtt cataacagtt ggtcagttcg atcgccagct
    24721 tttcgcactg agtttcaatg ccgtggcgga tcgccagata gtaaggatcg ttgatctcca
    24781 gctcctgctg gtagctgtag atagccagaa tatggtgttg gttgactgcg cctgtctgta
    24841 gtttacgggc gctactggtc ttgtactcca gcttttcggc gatctcgaga atgcgatgtt
    24901 tcgtctcttc tttcacattc aatgtcggat cgtcatttaa gaccctggat actgtcgcca
    24961 gggatacgcc agcttcgatt gcgatgtctt ttaatgttgc catgtttacc ttttttactt
    25021 tccctgatgt gcgcccccat tgtagaggaa gtgcttatcg ggtcacctgt tttcagtaaa
    25081 taaagcgcgt ttctttgacg ctgtaacggt aacacagcgg cttaggaatg tgatcacgcc
    25141 tgcattctcc atttttagcc tacaggattg agaaaaactt ttctatagtt tagtaaaatt
    25201 ttatccgaag ggaggaagca atggaaaccg tggcttacgc tgattttgca cgtctggaaa
    25261 tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag ctgtacatcg
    25321 tacaggttga tgtggggaaa aaacactgca aaccgtgacc agcctggtgc cgtactacag
    25381 cgaagaggag ctgatgggga aaacggtggt ggtcttatgc aatctgcaaa aagcgaaaat
    25441 gcgtggtgaa acgtcggaat gcatgctgtt gtgtgcggaa accgatgacg gcagtgaaag
    25501 cgtgttgtta acgccggagc ggatgatgcc tgcgggcgta cgcgtcgtat aaaaagatcg
    25561 gatggcgacg tcgtatcgcc atccgatttg atattacgct tcttcgacac ttactcgcat
    25621 ggccgccagc gccttacgcg ccgctttgat caccagttca cactgttcaa tggtcagtgt
    25681 cagtggcggt tcaatgcgga tcgttttggc gttattgagc gttccggcca ccagtacgcg
    25741 ctggcggaac atctcgctgg caaagttata gccgatttcg ttatcaacaa actcaatcgc
    25801 catcaacatc cctttaccac gcgcttcctg taccagatcg ggatattccc gcgccagttg
    25861 acggaaaccg tccagcaaca tatcgccttt ttgctcagcc tgagccggta agttctgctc
    25921 cagcaacaca ttgatggtcg ccagcgccgc cgcacaggcc agcgggttgc cgccaaaggt
    25981 ggtggtatgc aggaatgggt tgtcgaacag aactgaaaac acctcttcag tggcgatggt
    26041 cgcgccaatc ggcatcacgc cgccgccgag ggcttttgcc agacacagaa tgtccggctg
    26101 aacgttctca tgttcgcagg caaacatctt gcccgtgcgc cccatgcccg tttgtacttc
    26161 atcgaggatc atcagtgcgc cgaactcatc gcacagctta cgcacggcgg tgagatagcc
    26221 cggcggcggc agaattacgc caccttcgcc ctgaatcggt tcgaggatca ccgcagccac
    26281 atcatcaccg gtttttttgc actcattaag agccgtgcgc atggcttcga tattgccaaa
    26341 cggcacatga cggaaccctg gcagcagtgg cataaacggt ttgcggaagg tcgatttcgc
    26401 cgtggccgac agcgcgccaa gtgatttacc gtggaacgcg ccgctggtgg caataaaagt
    26461 aaatttgccg cgcggcgact gataagcctt cgccagcttc agcgccgctt caacggactc
    26521 ggtgccgcta ttacagaaga agctgtattt cagtttaccg ggcgttagcg cagcaagggt
    26581 tttcgccagc atagcccgta acggatcgag tagctcctgg ctgtgcagtg gttgtttcgc
    26641 aagttgattc tgtacggcgg aaaccacaac tggattacgg tgccccacgt tgaaaattcc
    26701 aaaacctccc aggcagtcga taaactcctg tccctgggtg tcgacaagcg tatttaaacc
    26761 tcccgcttgc cactctacgg ctccgtaatc cccgccggcg gtaacagatt tgcgatactc
    26821 taaaaacccc ggattgacat gctctttgaa gtattcaatc acctctcggt taagtgcttt
    26881 catctcctca tgatccagcg ttcgcttctc aatgagattc agggcgtggg cgctgcacgc
    26941 taaagccgat gcgctcgaag gtaacctgtt caaaatatgc tccggaggct cgcgtatcac
    27001 atgataccga tttaagtatt gcagggattg cgccactccg gctgcgatcg cgaaaatcgg
    27061 gataaacaca aatgcggtta tcatttgtgc aatatttaat gctatccgtt aacatttgtt
    27121 gttattttgc acggtttttg tcgggtatgg atttgatgat tgctgcgcaa atgcacgttt
    27181 tcagggcaat aattgcctga tttttgggca agataattaa cgttttcgta tagtaacaga
    27241 gtgaaagtat tttaaatcaa agaattagat ttgtgcttta atcgcaaatt gcgatctaaa
    27301 tcaaattaat cggttaaaga taaccgcagc ggggccgaca taaactctga caagaagtta
    27361 acaaccatat aacctgcaca ggacgcgaac atgtcttctc atccgtatgt cacccagcaa
    27421 aataccccgc tggcggacga taccactctg atgtccacta ccgatctgca aagctatatc
    27481 actcatgcta atgacacttt tgtgcaggtg agcggcttta ccttgcaaga gttacaaggg
    27541 cagccgcaca acatggtgcg tcacccggat atgccaaaag cggcgtttgc ggatatgtgg
    27601 ttcaccctga aaaaagggga gccctggagc ggcatcgtga aaaatcgccg caaaaatggc
    27661 gaccattatt gggtgcgggc caatgcggta ccgatggtgc gcgagggaaa aatcagtggc
    27721 tatatgtcga ttcgtacccg ggcgacggat gaagagattg cggcggtgga gccgctgtac
    27781 aaagcgttga actccggacg taccagtaag cgtattcata aaggcctggt ggtgcgtaaa
    27841 ggctggctgg gtaaactgcc ttcattaccg cttcgctggc ggacgcgtgg agtgatgacc
    27901 ctgatgttta tcttgctggc ggccatgctt tggtttgttg ctgccccggt ggtgacgtat
    27961 ttcctctgtg tgttagtggt attgttggca agcgcttgtt ttgaatggca gattgtgcgc
    28021 ccgatagaaa atgtcgcccg tcaggcactg aaggtggcga ccggagagcg taatagtgtt
    28081 gagcacctga atcgcagcga tgagctgggg ctgacattac gcgcggtagg gcagcttggc
    28141 ctgatgtgcc gttggctaat taacgatgtc tcaagccagg tgtccagtgt cagaaatggc
    28201 agtgagacgc tggcgaaagg caccgatgaa ctgaacgaac atacccagca gacagttgat
    28261 aacgttcagc aaacggtggc gaccatgaac caaatggcgg cgtcggtgaa acagaactct
    28321 gccacggcgt cggctgccga taaactttct atcaccgcca gtaatgcggc agtgcagggt
    28381 ggggaggcga tgaccacggt gatcaagaca atggacgata tcgccgacag tacctagcgc
    28441 attggcacca ttacttcgct gattaacgat attgcgtttc agaccaatat tctggccctg
    28501 aatgcggcgg tggaagcggc gcgtgccggc gaacagggca aaggttttgc agtggtggca
    28561 ggggaagtgc gtcatttagc cagccgcagc gctaatgctg ccaacgatat tcgcaagctg
    28621 attgatgcca gtgctgataa ggtgcaatcc ggttcgcagc aggtacacgc cgccggacgg
    28681 acgatggaag atattgtggc acaggtgaaa aacgtcaccc agttgatcgc ccagattagc
    28741 cattcaacgc tggaacaggc cgatgggctt tccagcctga cccgtgcagt ggatgagctt
    28801 aacctgatca cccagaaaaa tgccgagctg gtggaagaga gtgcgcaggt gtcggcgatg
    28861 gtgaaacacc gcgccagccg actggaagac gcggtgacgg tgctgcatta atcggtatgc
    28921 cggatccggc ggttggagca caatgcctga tgcgatgctg gcgcatctta tcaggcctgc
    28981 ggggtgtgta gcgggtgtag gccggataag gcgcgtcaag cgtcgcatcc ggcaattgca
    29041 ccgcgccact ggcggatgcg gcgattaatc cagttgcgta atatcaaaag cggcgcggtc
    29101 gatcaccgca atgatctttt ttatttgtgc atcactgata tcgctctggt tgacgcgtaa
    29161 atccagcacc gctttaaaat tatccagcgc ccgcttcatt tgcgggttct ggcgcagcgc
    29221 cgcgccaacg caacgcgctt tgatgcgttc ttcaatcatc tccacttgtt cgcggttttc
    29281 ttccagccac tgcgcacctt gttcggtcag cgcaatctgc ttcttaccac cttcctcttc
    29341 gcggatggta atcagcgact gctcctgcag aaaatccagc gtcgggtaga tgacgcccgg
    29401 gcttggggtg taattcccct gggttagatt ctcaatcgct ttaatcaatt cgtaaccgtg
    29461 gctgtcatcg cgcgagagaa tatccagaat caccagacgc aattcaccgt gaccaaagaa
    29521 acgttgccga cgaccgccgc cgcgaccgtg gcgaccaccg caacattgac cgtgttcatg
    29581 ctggtgaccg tgtccgcagt gctcgtgttc tgacttctca cctttgcagc agccctcatg
    29641 ccgtggctgg ccttcatgtt tacaacaccc ttcgtgatga tggctcatat cagcctcctt
    29701 ttgcttgatt agatatatct aatttatatc tgatgtgagc attactgcaa gtacaaaaat
    29761 gatttttaaa cgtggaagca cctcacatct aggccggata agacgttcac gccgcatccg
    29821 gcagcattgc tatggcactg aatgccttca catacttcag acatatcagt tctacaaatc
    29881 gcttgcattt atcatgatta acaatcatta tcatttgcga attttattta gatatatctg
    29941 attaccatca cgaaggcgat aacaatgaat aacacccccc gctacccgca gcgcgttcgc
    30001 aatgatctgc gcttccgtga actgactgtg ctacgcgttg agcgcatcag cgccggtttt
    30061 cagcgcattg tccttggcgg cgaggcgttg gacggtttta catcgcgcgg ctttgacgat
    30121 cacagcaaac tcttctttcc tcaacctgac gctcactttg tgccgccaac ggtaacggaa
    30181 gagagcatcg tctggccgga aggaccacgc ccaccgtcgc gtgactatac gccactgtat
    30241 gacgaactac gccatgaact ggcgatcgat ttctttattc acgacggtgg ggtcgccagc
    30301 ggctgggcga taaacttacg gtggcaggtc cgcgcggttc gctggtggtg ccggaagatt
    30361 acgcgtatca gctgtatgtc tgcgatgaat ccggaatgcc cgcactgcgc cgccgcctgg
    30421 aaacgttgag caaacttgcc gttaaaccgc aagttagtgc gctggttagc gtgcgggata
    30481 acgcctgtca ggattatctc gcgcaccttg atggttttaa tatcgaatgg ctggcacatg
    30541 atgagcaggc ggtagatgcg cgtctggcgc agatgcaaat ccctgccgat gattacttca
    30601 tctggataac cggcgaaggt aaagtcgtta agaatttaag ccgccgcttt gaagcggaac
    30661 agtatgaccc acagcgggta cgtgcagcgg cttactggca cgcaaagtaa ctgatatata
    30721 aaaagaaaaa ggctgacgat ttctcgtcag cctttgctat atctggtggc ccctgctgga
    30781 cttgaaccag cgaccaagcg attatgagtc gcctgctcta accactgagc taaggggcca
    30841 tggtagcgga ttataaagta actccgtgtc gcaatccagc cattaccgcg cgcctgctgt
    30901 ttttataaac aatgtatttt caatccgtta tacttttcct ggtgatgtta gaaaggagta
    30961 aatatggttg aggatatttt ggctccaggg ttacgggtcg tgttttgcgg tatcaaccct
    31021 gggctttcat ccgccgggac tggttttccc tttgctcatc cggcaaatcg cttctggaag
    31081 gtgatatatc aggccgggtt taccgaccgt cagttgaagc cgcaggaggc acagcatctg
    31141 ctggattatc gttgtggcat caccaaactg gtagaccgtc caacggtgca agccaatgaa
    31201 gtttcaaagc aggagctgca cgcaggtggg cgtaagctga ttgaaaaaat tgaagattat
    31261 cagccgcagg cgttggcgat tctgggcaaa caagcatatg aacagggatt cagctagcgc
    31321 ggtgcacagt gggggaaaca aacgctcacc attggttcga cgcagatttg ggtgctgcca
    31381 aatcccagcg gtttaagtcg cgtttcactg gagaaactgg ttgaagcgta tcgcgagctg
    31441 gaccaggcgc tggtagtgcg tgggcgataa
//
LOCUS       DBKELA010000041        31328 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.041, whole genome shotgun sequence.
ACCESSION   DBKELA010000041 DBKELA010000000
VERSION     DBKELA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.041"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            698..1126
                     /gene="uspC"
                     /locus_tag="ACUQR0_001859"
     CDS             698..1126
                     /gene="uspC"
                     /locus_tag="ACUQR0_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HHV9108926.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="ACUQR0_001860"
                     /pseudo
     CDS             complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="ACUQR0_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="ACUQR0_001861"
     CDS             complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="ACUQR0_001861"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HHV9108927.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3489..4475)
                     /gene="araH"
                     /locus_tag="ACUQR0_001862"
     CDS             complement(3489..4475)
                     /gene="araH"
                     /locus_tag="ACUQR0_001862"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HHV9108928.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4490..6004)
                     /gene="araG"
                     /locus_tag="ACUQR0_001863"
     CDS             complement(4490..6004)
                     /gene="araG"
                     /locus_tag="ACUQR0_001863"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HHV9108929.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6074..7063)
                     /gene="araF"
                     /locus_tag="ACUQR0_001864"
                     /pseudo
     CDS             complement(6074..7063)
                     /gene="araF"
                     /locus_tag="ACUQR0_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7860..8363
                     /locus_tag="ACUQR0_001865"
     CDS             7860..8363
                     /locus_tag="ACUQR0_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HHV9108930.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8443..8694)
                     /gene="yecJ"
                     /locus_tag="ACUQR0_001866"
     CDS             complement(8443..8694)
                     /gene="yecJ"
                     /locus_tag="ACUQR0_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HHV9108931.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="ACUQR0_001867"
     CDS             complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="ACUQR0_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HHV9108932.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9158..9481
                     /locus_tag="ACUQR0_001868"
     CDS             9158..9481
                     /locus_tag="ACUQR0_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HHV9108933.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9652..10149
                     /gene="ftnA"
                     /locus_tag="ACUQR0_001869"
     CDS             9652..10149
                     /gene="ftnA"
                     /locus_tag="ACUQR0_001869"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HHV9108934.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10186..10425)
                     /locus_tag="ACUQR0_001870"
     CDS             complement(10186..10425)
                     /locus_tag="ACUQR0_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HHV9108935.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10615..11826
                     /gene="tyrP"
                     /locus_tag="ACUQR0_001871"
     CDS             10615..11826
                     /gene="tyrP"
                     /locus_tag="ACUQR0_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HHV9108936.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="ACUQR0_001872"
     CDS             complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="ACUQR0_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HHV9108937.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12749..12835)
                     /locus_tag="ACUQR0_001873"
     tRNA            complement(12749..12835)
                     /locus_tag="ACUQR0_001873"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Leu,seq:taa)
     gene            complement(12848..12921)
                     /locus_tag="ACUQR0_001874"
     tRNA            complement(12848..12921)
                     /locus_tag="ACUQR0_001874"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12887..12889),aa:Cys,seq:gca)
     gene            complement(12976..13051)
                     /locus_tag="ACUQR0_001875"
     tRNA            complement(12976..13051)
                     /locus_tag="ACUQR0_001875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Gly,seq:gcc)
     gene            complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="ACUQR0_001876"
     CDS             complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="ACUQR0_001876"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HHV9108938.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="ACUQR0_001877"
     CDS             complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="ACUQR0_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HHV9108939.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="ACUQR0_001878"
     CDS             complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="ACUQR0_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HHV9108940.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16435..16506
                     /locus_tag="ACUQR0_001879"
                     /pseudo
     CDS             16435..16506
                     /locus_tag="ACUQR0_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            16589..16765
                     /gene="yecU"
                     /locus_tag="ACUQR0_001880"
     CDS             16589..16765
                     /gene="yecU"
                     /locus_tag="ACUQR0_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HHV9108941.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16752..16976
                     /gene="yecF"
                     /locus_tag="ACUQR0_001881"
     CDS             16752..16976
                     /gene="yecF"
                     /locus_tag="ACUQR0_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HHV9108942.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="ACUQR0_001882"
                     /pseudo
     CDS             complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="ACUQR0_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17859..18611)
                     /gene="tcyN"
                     /locus_tag="ACUQR0_001883"
     CDS             complement(17859..18611)
                     /gene="tcyN"
                     /locus_tag="ACUQR0_001883"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HHV9108943.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="ACUQR0_001884"
     CDS             complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="ACUQR0_001884"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HHV9108944.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="ACUQR0_001885"
     CDS             complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="ACUQR0_001885"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HHV9108945.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="ACUQR0_001886"
     CDS             complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="ACUQR0_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9108946.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="ACUQR0_001887"
     CDS             complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="ACUQR0_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HHV9108947.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="ACUQR0_001888"
     CDS             complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="ACUQR0_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HHV9108948.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="ACUQR0_001889"
     CDS             complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="ACUQR0_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HHV9108949.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24669..26075
                     /gene="fliD"
                     /locus_tag="ACUQR0_001890"
     CDS             24669..26075
                     /gene="fliD"
                     /locus_tag="ACUQR0_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HHV9108950.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26100..26510
                     /gene="fliS"
                     /locus_tag="ACUQR0_001891"
     CDS             26100..26510
                     /gene="fliS"
                     /locus_tag="ACUQR0_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HHV9108951.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26510..26875
                     /gene="fliT"
                     /locus_tag="ACUQR0_001892"
     CDS             26510..26875
                     /gene="fliT"
                     /locus_tag="ACUQR0_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HHV9108952.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26953..28440
                     /gene="amyA"
                     /locus_tag="ACUQR0_001893"
     CDS             26953..28440
                     /gene="amyA"
                     /locus_tag="ACUQR0_001893"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HHV9108953.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28474..28887)
                     /gene="yedD"
                     /locus_tag="ACUQR0_001894"
     CDS             complement(28474..28887)
                     /gene="yedD"
                     /locus_tag="ACUQR0_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HHV9108954.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29074..30279
                     /gene="yedE"
                     /locus_tag="ACUQR0_001895"
     CDS             29074..30279
                     /gene="yedE"
                     /locus_tag="ACUQR0_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HHV9108955.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30276..30509
                     /gene="yedF"
                     /locus_tag="ACUQR0_001896"
     CDS             30276..30509
                     /gene="yedF"
                     /locus_tag="ACUQR0_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HHV9108956.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30616..31286
                     /locus_tag="ACUQR0_001897"
                     /pseudo
     CDS             30616..31286
                     /locus_tag="ACUQR0_001897"
                     /inference="COORDINATES: protein motif:HMM:NF014626.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         7892 a         7991 c         7401 g         8044 t
ORIGIN      
        1 ttagcattag aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa
       61 gtccttacaa atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
      121 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
      181 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
      241 tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
      301 gaataatcga aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc
      361 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
      421 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt
      481 tcatatatta accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag
      541 ttgaaatcat atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa
      601 tatttacctg tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
      661 tgtcacctga agttcacagc agaataagga acaggttatg agctatagca atattcttgt
      721 cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag
      781 gccagtaaag ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca
      841 attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt
      901 tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg
      961 tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg
     1021 cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc
     1081 ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa
     1141 gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct
     1201 gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt
     1261 ccagcatttc tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca
     1321 gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt
     1381 ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag
     1441 caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt
     1501 cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa
     1561 gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct
     1621 gatgacgaat atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat
     1681 aacgaatttt accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac
     1741 gctccggcaa acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt
     1801 tcaattccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta
     1861 tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg
     1921 tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg
     1981 ccagacgatc gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca
     2041 aggtgtcata tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga
     2101 aaaaaccaat gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt
     2161 gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca
     2221 gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga
     2281 gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa
     2341 ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc
     2401 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2461 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2521 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2581 gtaatgcggc ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2641 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2701 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2761 aaggagcttc ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga
     2821 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2881 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2941 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca
     3001 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     3061 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     3121 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     3181 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3241 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3301 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca
     3361 gaccgcgctt tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga
     3421 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3481 aaaaagcatc agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3541 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3601 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3661 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3721 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3781 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc
     3841 agacgcgcag cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta
     3901 ttcagcagca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3961 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     4021 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc
     4081 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc
     4141 agcaacaaac ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     4201 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4261 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4321 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4381 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4441 ggtgcgccag accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4501 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4561 acaattcacc ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4621 cttcaggtaa gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa
     4681 ttacgttata tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca
     4741 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4801 ctgagagatt catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt
     4861 gctgatcggc attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt
     4921 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4981 ctttgcgagc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     5041 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     5101 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     5161 cactgcgaac cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac
     5221 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5281 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5341 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5401 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5461 gattgccgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5521 cgttacgcgc cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt
     5581 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5641 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5701 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5761 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5821 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5881 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5941 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     6001 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     6061 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     6121 cacgcgtgat cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg
     6181 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6241 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt
     6301 taataccaat accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc
     6361 cgccaagtac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6421 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6481 cctgataaat ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg
     6541 tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga
     6601 catcccagcc acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt
     6661 tagtcgccgc catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa
     6721 actggtcatc cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6781 agccgagttt cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc
     6841 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6901 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6961 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     7021 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     7081 gtgtcgtttt gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     7141 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     7201 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7261 ggaaaagaca taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa
     7321 tccataagat tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt
     7381 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac
     7441 aactcaaaga tgtggaaaat gcgcgtcatt catttcgcca ttaattatca ctgtgctcat
     7501 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7561 atcatcactt catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac
     7621 tatcattaaa attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa
     7681 cttgatgatt taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg
     7741 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7801 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7861 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7921 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7981 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     8041 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga
     8101 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc
     8161 tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     8221 gcgatctgga aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag
     8281 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8341 tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt
     8401 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     8461 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     8521 gtctgcggca gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc
     8581 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     8641 gcaagctacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     8701 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     8761 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     8821 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     8881 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     8941 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     9001 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     9061 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     9121 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     9181 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     9241 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     9301 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     9361 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     9421 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     9481 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     9541 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     9601 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     9661 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     9721 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     9781 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     9841 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     9901 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     9961 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
    10021 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
    10081 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
    10141 caaaactaat gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat
    10201 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    10261 aaacgctacc agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg
    10321 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10381 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10441 aaagcctagc gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10501 tttattgtac atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg
    10561 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    10621 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    10681 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    10741 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    10801 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    10861 acgggcttca gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc
    10921 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    10981 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
    11041 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    11101 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    11161 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    11221 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt
    11281 gccatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    11341 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
    11401 cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc
    11461 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
    11521 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
    11581 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    11641 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
    11701 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    11761 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg
    11821 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg
    11881 tgacctctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
    11941 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    12001 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
    12061 caaacgcttc tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    12121 caatcgcctc cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca
    12181 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    12241 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    12301 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac
    12361 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt
    12421 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    12481 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    12541 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    12601 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
    12661 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    12721 tactttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    12781 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
    12841 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
    12901 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
    12961 cgatgtcttt taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa
    13021 ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
    13081 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
    13141 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
    13201 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
    13261 gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga
    13321 cgccacagca gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
    13381 gagacggcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
    13441 ataatttcac gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc
    13501 tcggttacca gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa
    13561 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
    13621 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
    13681 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
    13741 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
    13801 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
    13861 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
    13921 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
    13981 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
    14041 gaatatgctg gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt
    14101 ccggctcaaa gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa
    14161 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
    14221 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
    14281 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
    14341 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
    14401 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
    14461 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
    14521 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
    14581 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
    14641 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
    14701 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct
    14761 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
    14821 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
    14881 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
    14941 gaatttggtc gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca
    15001 tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
    15061 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
    15121 agcgccctat ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
    15181 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
    15241 tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac
    15301 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
    15361 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
    15421 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
    15481 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
    15541 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
    15601 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
    15661 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
    15721 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
    15781 tgagatctca ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc
    15841 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
    15901 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
    15961 gacttcctgc ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt
    16021 cgctggtaaa gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga
    16081 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
    16141 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
    16201 tccggcgact tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag
    16261 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
    16321 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
    16381 aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc
    16441 acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac
    16501 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
    16561 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
    16621 cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa
    16681 gcttatggta tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac
    16741 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
    16801 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
    16861 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
    16921 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
    16981 aaaccggctg agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg
    17041 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
    17101 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
    17161 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
    17221 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
    17281 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
    17341 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
    17401 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
    17461 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
    17521 gctgctgagc ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa
    17581 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
    17641 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
    17701 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
    17761 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
    17821 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg
    17881 aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc
    17941 tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc
    18001 atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc
    18061 aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc
    18121 tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
    18181 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
    18241 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
    18301 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
    18361 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
    18421 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat
    18481 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
    18541 aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca
    18601 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
    18661 acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
    18721 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
    18781 ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca
    18841 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac
    18901 caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg
    18961 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
    19021 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
    19081 gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
    19141 acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac
    19201 tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
    19261 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
    19321 aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
    19381 gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
    19441 ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    19501 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    19561 cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac
    19621 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    19681 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    19741 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    19801 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc
    19861 cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc
    19921 caacaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    19981 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    20041 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    20101 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc
    20161 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    20221 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    20281 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    20341 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    20401 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    20461 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    20521 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    20581 gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg
    20641 gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct
    20701 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    20761 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    20821 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    20881 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    20941 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    21001 ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg
    21061 ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    21121 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    21181 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    21241 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    21301 gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg
    21361 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    21421 ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc
    21481 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    21541 ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    21601 aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc
    21661 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    21721 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    21781 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    21841 aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    21901 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    21961 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    22021 aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc
    22081 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc
    22141 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg
    22201 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    22261 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    22321 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    22381 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg
    22441 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    22501 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    22561 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    22621 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    22681 ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc
    22741 attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg
    22801 ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg
    22861 tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt
    22921 tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt
    22981 tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt
    23041 ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc
    23101 cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg
    23161 cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt
    23221 tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat
    23281 cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag
    23341 tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat
    23401 tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg
    23461 ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat
    23521 cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat
    23581 caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa
    23641 aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga
    23701 agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag
    23761 acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat
    23821 tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca
    23881 cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag
    23941 tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca
    24001 cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt
    24061 cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca
    24121 gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg
    24181 caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag
    24241 taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc
    24301 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    24361 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    24421 tgcaagtcgt tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt
    24481 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    24541 cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    24601 aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag
    24661 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    24721 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    24781 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    24841 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca
    24901 gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca
    24961 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    25021 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc
    25081 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca
    25141 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    25201 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    25261 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    25321 tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca
    25381 gcaacactat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    25441 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    25501 gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg
    25561 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    25621 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    25681 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    25741 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    25801 ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc
    25861 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt
    25921 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag
    25981 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    26041 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    26101 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga
    26161 gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    26221 gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    26281 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    26341 taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    26401 tacgcaacga tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg
    26461 tctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc
    26521 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    26581 ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt
    26641 gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct
    26701 ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    26761 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    26821 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt
    26881 aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa
    26941 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    27001 ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc
    27061 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac
    27121 gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa
    27181 tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg
    27241 gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    27301 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    27361 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    27421 ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa
    27481 attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat
    27541 ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt
    27601 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg
    27661 gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    27721 ccaaagccgc tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg
    27781 tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc
    27841 catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg
    27901 gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg
    27961 caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    28021 ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa
    28081 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    28141 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat
    28201 ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc
    28261 atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa
    28321 acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa
    28381 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    28441 atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    28501 tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc
    28561 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    28621 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca
    28681 ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    28741 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    28801 gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    28861 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    28921 ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    28981 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt
    29041 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    29101 tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact
    29161 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    29221 ggtcagctcc tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc
    29281 catctggaag gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc
    29341 ggctgctttg ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt
    29401 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    29461 atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg
    29521 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    29581 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    29641 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    29701 tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc
    29761 ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg
    29821 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc
    29881 atcgggatct tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca
    29941 aatgcggtaa ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa
    30001 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    30061 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    30121 accgactggg acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg
    30181 acatatttgc tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    30241 ccccgtgagg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    30301 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc
    30361 cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata
    30421 atattccact ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc
    30481 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    30541 acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    30601 tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt
    30661 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    30721 tacaatgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    30781 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    30841 gcccgcgtag aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg
    30901 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt
    30961 attttatcta tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt
    31021 ttgagcgtgg tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc
    31081 tggtagatat tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga
    31141 tgcggcaaga cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag
    31201 caaaccagtt cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg
    31261 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc
    31321 tacgttca
//
LOCUS       DBKELA010000042        30584 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.042, whole genome shotgun sequence.
ACCESSION   DBKELA010000042 DBKELA010000000
VERSION     DBKELA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.042"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..588
                     /locus_tag="ACUQR0_001898"
     CDS             <1..588
                     /locus_tag="ACUQR0_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HHV9108957.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACUQR0_001899"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACUQR0_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HHV9108958.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACUQR0_001900"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACUQR0_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HHV9108959.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACUQR0_001901"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACUQR0_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HHV9108960.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACUQR0_001902"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACUQR0_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HHV9108961.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACUQR0_001903"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACUQR0_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HHV9108962.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACUQR0_001904"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACUQR0_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HHV9108963.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACUQR0_001905"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACUQR0_001905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HHV9108964.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACUQR0_001906"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACUQR0_001906"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HHV9108965.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACUQR0_001907"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACUQR0_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HHV9108966.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACUQR0_001908"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACUQR0_001908"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HHV9108967.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACUQR0_001909"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACUQR0_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HHV9108968.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACUQR0_001910"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACUQR0_001910"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HHV9108969.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACUQR0_001911"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACUQR0_001911"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HHV9108970.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACUQR0_001912"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACUQR0_001912"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HHV9108971.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACUQR0_001913"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACUQR0_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HHV9108972.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="ACUQR0_001914"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="ACUQR0_001914"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HHV9108973.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="ACUQR0_001915"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="ACUQR0_001915"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HHV9108974.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="ACUQR0_001916"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="ACUQR0_001916"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HHV9108975.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="ACUQR0_001917"
     CDS             22192..22782
                     /locus_tag="ACUQR0_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HHV9108976.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="ACUQR0_001918"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="ACUQR0_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HHV9108977.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="ACUQR0_001919"
     CDS             23254..23949
                     /locus_tag="ACUQR0_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HHV9108978.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACUQR0_001920"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACUQR0_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HHV9108979.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="ACUQR0_001921"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="ACUQR0_001921"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HHV9108980.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATLLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="ACUQR0_001922"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="ACUQR0_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HHV9108981.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWSYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACUQR0_001923"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACUQR0_001923"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HHV9108982.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACUQR0_001924"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACUQR0_001924"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HHV9108983.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7166 a         7965 c         8417 g         7036 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggcat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgattcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacttg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatgtcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggcc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cgctcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg agctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gaga
//
LOCUS       DBKELA010000043        29799 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.043, whole genome shotgun sequence.
ACCESSION   DBKELA010000043 DBKELA010000000
VERSION     DBKELA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.043"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..852
                     /locus_tag="ACUQR0_001925"
     CDS             <1..852
                     /locus_tag="ACUQR0_001925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HHV9108984.1"
                     /translation="IRHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGF
                     NILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAE
                     IVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHE
                     IYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHN
                     PLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTG
                     TIYPIEK"
     gene            874..2283
                     /locus_tag="ACUQR0_001926"
     CDS             874..2283
                     /locus_tag="ACUQR0_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9108985.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="ACUQR0_001927"
     CDS             2261..3040
                     /locus_tag="ACUQR0_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HHV9108986.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="ACUQR0_001928"
     CDS             3037..3897
                     /locus_tag="ACUQR0_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HHV9108987.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="ACUQR0_001929"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="ACUQR0_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="ACUQR0_001930"
     CDS             complement(4637..4897)
                     /locus_tag="ACUQR0_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HHV9108988.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="ACUQR0_001931"
     CDS             complement(4888..6159)
                     /locus_tag="ACUQR0_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHV9108989.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="ACUQR0_001932"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="ACUQR0_001932"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HHV9108990.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="ACUQR0_001933"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="ACUQR0_001933"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HHV9108991.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="ACUQR0_001934"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="ACUQR0_001934"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HHV9108992.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="ACUQR0_001935"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="ACUQR0_001935"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HHV9108993.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="ACUQR0_001936"
     CDS             11501..11653
                     /locus_tag="ACUQR0_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HHV9108994.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11847..12950
                     /locus_tag="ACUQR0_001937"
                     /pseudo
     CDS             11847..12950
                     /locus_tag="ACUQR0_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13084..14121)
                     /gene="iap"
                     /locus_tag="ACUQR0_001938"
     CDS             complement(13084..14121)
                     /gene="iap"
                     /locus_tag="ACUQR0_001938"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HHV9108995.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14373..15281
                     /gene="cysD"
                     /locus_tag="ACUQR0_001939"
     CDS             14373..15281
                     /gene="cysD"
                     /locus_tag="ACUQR0_001939"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HHV9108996.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15283..16710
                     /gene="cysN"
                     /locus_tag="ACUQR0_001940"
     CDS             15283..16710
                     /gene="cysN"
                     /locus_tag="ACUQR0_001940"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HHV9108997.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16710..17315
                     /gene="cysC"
                     /locus_tag="ACUQR0_001941"
     CDS             16710..17315
                     /gene="cysC"
                     /locus_tag="ACUQR0_001941"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HHV9108998.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17365..17688
                     /locus_tag="ACUQR0_001942"
     CDS             17365..17688
                     /locus_tag="ACUQR0_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HHV9108999.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17882..18193
                     /gene="ftsB"
                     /locus_tag="ACUQR0_001943"
     CDS             17882..18193
                     /gene="ftsB"
                     /locus_tag="ACUQR0_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HHV9109000.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18212..18922
                     /gene="ispD"
                     /locus_tag="ACUQR0_001944"
     CDS             18212..18922
                     /gene="ispD"
                     /locus_tag="ACUQR0_001944"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HHV9109001.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18922..19401
                     /gene="ispF"
                     /locus_tag="ACUQR0_001945"
     CDS             18922..19401
                     /gene="ispF"
                     /locus_tag="ACUQR0_001945"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HHV9109002.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19398..20447
                     /gene="truD"
                     /locus_tag="ACUQR0_001946"
     CDS             19398..20447
                     /gene="truD"
                     /locus_tag="ACUQR0_001946"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HHV9109003.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20428..21189
                     /gene="surE"
                     /locus_tag="ACUQR0_001947"
     CDS             20428..21189
                     /gene="surE"
                     /locus_tag="ACUQR0_001947"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HHV9109004.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21183..21809
                     /gene="pcm"
                     /locus_tag="ACUQR0_001948"
     CDS             21183..21809
                     /gene="pcm"
                     /locus_tag="ACUQR0_001948"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HHV9109005.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21994..23088
                     /gene="nlpD"
                     /locus_tag="ACUQR0_001949"
     CDS             21994..23088
                     /gene="nlpD"
                     /locus_tag="ACUQR0_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HHV9109006.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23151..24143
                     /gene="rpoS"
                     /locus_tag="ACUQR0_001950"
     CDS             23151..24143
                     /gene="rpoS"
                     /locus_tag="ACUQR0_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HHV9109007.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24263..24670)
                     /locus_tag="ACUQR0_001951"
     CDS             complement(24263..24670)
                     /locus_tag="ACUQR0_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHV9109008.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24817..25410
                     /locus_tag="ACUQR0_001952"
     CDS             24817..25410
                     /locus_tag="ACUQR0_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HHV9109009.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25410..26357
                     /locus_tag="ACUQR0_001953"
                     /pseudo
     CDS             25410..26357
                     /locus_tag="ACUQR0_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26630..27193)
                     /locus_tag="ACUQR0_001954"
     CDS             complement(26630..27193)
                     /locus_tag="ACUQR0_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109010.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27358..27609
                     /locus_tag="ACUQR0_001955"
     CDS             27358..27609
                     /locus_tag="ACUQR0_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9109011.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27611..29707
                     /locus_tag="ACUQR0_001956"
     CDS             27611..29707
                     /locus_tag="ACUQR0_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HHV9109012.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7301 a         7382 c         8058 g         7058 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggcgt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggct gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac cagccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcttggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt
    11761 attatgaatt tcaaatgaaa atcattaatt tattcataat gtaaatgttt tattggctga
    11821 tatatatcat agatagtttt taaaatatgc gtaaatatcc tttaagttta ctgaaggata
    11881 aaaatattgt gactttcttt gatttctggg gaaaaaaccg acgtggcgag aaagagggta
    11941 tcgacgacta tcaccttctt tgctggcatt tgctggatgt ggccgcaatg ggctatttaa
    12001 tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca
    12061 aggaacaggc agctcaattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg
    12121 cccgctcttt tcaacaactt tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa
    12181 aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa
    12241 gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga
    12301 tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg
    12361 aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca
    12421 agccactgtt tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac
    12481 ttataaaaca actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt
    12541 cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa
    12601 aggctttagt tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc
    12661 ctttcaccgg aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa
    12721 aggtgctgga tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga
    12781 ccggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta
    12841 acgctcaggg gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc
    12901 gactggtcaa aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga
    12961 taaaccggcg caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg
    13021 atgggtatca cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac
    13081 ccatcaagac gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg
    13141 gcagcgacgt tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct
    13201 tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc
    13261 atccttattt ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt
    13321 gtcgaatatt tctgcgtcat tacaacaccc agtgcctttc ggataatttt tattcaaacc
    13381 cggattggtc gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt
    13441 taatttcctt actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac
    13501 aattaagtta tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg
    13561 cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg
    13621 aataccatac tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa
    13681 acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc
    13741 agcatcgctc agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc
    13801 tttgccttca tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt
    13861 atcgcgggcg gtataaatat accgactatt aaatgtccga atatcactgc gataacccat
    13921 ttgctgaaac tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat
    13981 gcgtcccgga aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt
    14041 aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg
    14101 gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca
    14161 tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg
    14221 gtgccttaag cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact
    14281 cattctaatt ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg
    14341 caagaaaata gcggtattgc aaaggaacgg ttatggatca aaaacgactt actcacctgc
    14401 ggcaactgga ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc
    14461 cggtgatgct ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg
    14521 cgttttatcc aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc
    14581 gcgagatgta tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc
    14641 ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac
    14701 ataccgatat tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg
    14761 ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact
    14821 ctttccgtga ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca
    14881 actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga
    14941 ccgagcagga tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc
    15001 tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc
    15061 gtatcgacct gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg
    15121 gctgctggcc gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg
    15181 aagagatgct ggtttccacc accagtgaac gtcaggggcg cgtgattgac cgcgaccagg
    15241 cgggatccat ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca
    15301 caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc
    15361 ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt
    15421 ctgctgcacg atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt
    15481 aagcgtcacg gcacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa
    15541 gctgagcgcg aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag
    15601 cgtaaattta ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact
    15661 ggcgcatcga catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat
    15721 caaacccgtc gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg
    15781 atcaacaaaa tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat
    15841 tatctgacct ttgccgggca gctgccggat aatctggata tccgctttgt gccgctctcc
    15901 gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg
    15961 acactgctcg aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg
    16021 cgcttcccgg tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg
    16081 ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa
    16141 tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa
    16201 gcgatcaccc tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg
    16261 gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa
    16321 cagccgcttt ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct
    16381 cgtgttgatg gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa
    16441 aacctgccgc tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta
    16501 gatcgctatc agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat
    16561 gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct
    16621 gaattcagtg cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg
    16681 ggcgcgcgcg atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc
    16741 atagtcatcc ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc
    16801 tgtggtttac cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg
    16861 cgttacataa actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat
    16921 tatgcagcga tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg
    16981 aagtggcgaa tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac
    17041 accgcgccga acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt
    17101 ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag
    17161 cgcgtgccgg tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat
    17221 cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag
    17281 atctgttgag acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg
    17341 ttcgtcaaag taacaggatc aaatatgcgt aatagccata acattacact aacaaataac
    17401 gacagcctta ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt
    17461 atctcctggt tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc
    17521 ttctttctct acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg
    17581 attgcgctgc attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg
    17641 acagtaggca ttatgtttgg ctcactgttt atgtggctac tgggctaatt tgtactttct
    17701 tgtctcctct gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct
    17761 gggtatacgt aacaatggac aaatgtggta catttgcccg cgttgtcgcg gtatccccaa
    17821 cagaggatgt agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag
    17881 gatgggtaaa ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg
    17941 gttcggtaag aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca
    18001 agctacaaac gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct
    18061 caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc
    18121 gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca
    18181 aaacaatcga taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc
    18241 cgtggttccg gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct
    18301 ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg
    18361 ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct
    18421 ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct
    18481 ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc
    18541 ttgtttgcat caggatgacc tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg
    18601 agggatcctc gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc
    18661 cattgctcat accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc
    18721 tcgtgagctg ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga
    18781 cgaagcctcg gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga
    18841 taacattaaa gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac
    18901 catccatcag gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc
    18961 ggtgaaggcc caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg
    19021 cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg
    19081 ctgggggata tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc
    19141 cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac
    19201 gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc
    19261 gtgtttattg ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact
    19321 acggaaaaac ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta
    19381 ctcattaagg caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc
    19441 aaggcaccgg gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct
    19501 ttgagcctga tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata
    19561 cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct
    19621 tcgctgggca aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg
    19681 gcaaggaaat gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg
    19741 cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc
    19801 tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag
    19861 gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg
    19921 cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt
    19981 tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag
    20041 acgttaatca agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg
    20101 tagcaaccac cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga
    20161 taaccgccgc attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg
    20221 aacaagcagc tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg
    20281 aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg
    20341 acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca
    20401 gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatga tgacggggta
    20461 catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg
    20521 gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg
    20581 cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt
    20641 gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt
    20701 aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg
    20761 gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac
    20821 gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc
    20881 accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt
    20941 cgcgtgacgc gctgcggtac acgacatccg gcagatcagg tgatcccgca gcaagatccg
    21001 cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg
    21061 accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta
    21121 actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg
    21181 caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg
    21241 atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt
    21301 ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc
    21361 agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc
    21421 tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg
    21481 tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc
    21541 ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg
    21601 cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga
    21661 cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga
    21721 aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg
    21781 tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt
    21841 tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg
    21901 cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga
    21961 agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca
    22021 ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct
    22081 gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac gatgtctaca
    22141 gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg
    22201 caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc
    22261 gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc
    22321 gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac
    22381 cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag
    22441 gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca
    22501 gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa
    22561 ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac
    22621 aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc
    22681 gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact
    22741 gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc
    22801 gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt
    22861 aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt
    22921 gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa
    22981 aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt
    23041 tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc
    23101 aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga
    23161 atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg
    23221 tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg
    23281 aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg
    23341 agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac
    23401 tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa
    23461 aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca
    23521 acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa
    23581 catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta
    23641 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg
    23701 agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata
    23761 agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca
    23821 ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga
    23881 acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt
    23941 tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg
    24001 aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc
    24061 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata
    24121 tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc
    24181 tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc
    24241 gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca
    24301 gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag
    24361 gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct
    24421 tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt
    24481 ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag
    24541 gtttatcagc aatggcacgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt
    24601 gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc
    24661 gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac
    24721 gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata
    24781 ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca
    24841 ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat
    24901 gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct
    24961 tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg
    25021 gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg
    25081 aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac
    25141 gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc
    25201 atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg
    25261 cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc
    25321 gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg attaccgcag
    25381 gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac
    25441 aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg
    25501 atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt
    25561 ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc
    25621 agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg
    25681 agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct
    25741 gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc
    25801 gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc
    25861 cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg
    25921 gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata
    25981 aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca
    26041 tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg
    26101 cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt
    26161 ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc
    26221 cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg
    26281 ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga
    26341 ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa
    26401 agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt
    26461 ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac
    26521 aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc
    26581 aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg
    26641 ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc
    26701 taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta
    26761 gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc
    26821 tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta
    26881 gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt
    26941 caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct
    27001 tttttaactg atgtgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta
    27061 aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta
    27121 ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg
    27181 gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa
    27241 aataaaccat aaaaacacca aagaaccctt tacagggaat ctttggtatc ttaatcttta
    27301 tccatacgga tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg
    27361 agacaaagag aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa
    27421 gataacgggt ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca
    27481 ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata
    27541 ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag
    27601 gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc
    27661 agccaccaca aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt
    27721 tagtcgccgt cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat
    27781 aacacgaaag gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt
    27841 ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga
    27901 agcatatcgc ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca
    27961 gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc
    28021 cggtaacccc gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga
    28081 gctgccggag cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg
    28141 tactatcagc cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc
    28201 cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat
    28261 cagctggctg cctgaatttc tggcgcagta tcgccaggtg aacggcagac ccatgtcaga
    28321 agcctacgag gattttgtcg cagaatggca gcgtcgccat gcggacgaac cgtacatgct
    28381 ggaggtgatg ccttcctatg atgttgtccg gtatgccatg aaaaaactgc cggaagtggt
    28441 gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag cttgaaggat tcacccgccg
    28501 cgactggaca gctatgccgg tgaattatgt ctggattggt gacggtcacg gtatgaagct
    28561 gaaatgtgcg catccgatcc acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga
    28621 tggtggaaca cggtttgttg ttggctggag tcttgacctt gccgaaaacg tgttcgccgt
    28681 tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt attactcgga
    28741 taacggttcc ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta ttctgccacg
    28801 cctggggatt aaacacccta ccggtattgc cggtaatcca cagggacgcg gcattattga
    28861 acgcctgaac cggacactgc cgatgcgtat agccaggagg tatcgcacct attttggcaa
    28921 gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc
    28981 cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc
    29041 gtgggcggag cttattgagg ctattcgtga aggcgtggaa tggtacaaca accgcccgca
    29101 ttctgagctg ccgatgaaac cgaacggacg gcattacagc ccgacagagt tcagaaaaaa
    29161 acgccaggca gaagaagaca ccgaaattga gtggctgagt gatcttgagc tgcgcgacat
    29221 gttccgccca atggtggaac gaccggtaag acgctgcgaa attcagtggc tgaacaatat
    29281 ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat
    29341 tcatgacgcc gagcgaatta cggtacgtcg caaggacggc agctttatct gtgaggcgat
    29401 atggaacggc aataaacgcg cagcctttgc tgtcagtgct gaatatcaca aacaacagca
    29461 gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg
    29521 tattcagatt ctggaacaca aacaggctga accctggctg agtaatgttt atcgcccggt
    29581 tggtaatgtg gtggcagttc agcaaccgga atatgaagaa gagcatgacg aagaatttga
    29641 acgcgatttc cggctgggga tgcaaaaact ttttgccatg caggaagaag atgatccgct
    29701 ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa ataaaggaaa caaagctgat
    29761 ttgaaataac cgaggaaata ctatgactga tattgatga
//
LOCUS       DBKELA010000044        29613 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.044, whole genome shotgun sequence.
ACCESSION   DBKELA010000044 DBKELA010000000
VERSION     DBKELA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.044"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..165)
                     /locus_tag="ACUQR0_001957"
     CDS             complement(<1..165)
                     /locus_tag="ACUQR0_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HHV9109013.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKL"
     gene            complement(306..2339)
                     /gene="metG"
                     /locus_tag="ACUQR0_001958"
     CDS             complement(306..2339)
                     /gene="metG"
                     /locus_tag="ACUQR0_001958"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HHV9109014.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2471..3580
                     /gene="apbC"
                     /locus_tag="ACUQR0_001959"
     CDS             2471..3580
                     /gene="apbC"
                     /locus_tag="ACUQR0_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HHV9109015.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3842..4123
                     /locus_tag="ACUQR0_001960"
     CDS             3842..4123
                     /locus_tag="ACUQR0_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HHV9109016.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4416..4940
                     /locus_tag="ACUQR0_001961"
     CDS             4416..4940
                     /locus_tag="ACUQR0_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HHV9109017.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5021..5695
                     /locus_tag="ACUQR0_001962"
     CDS             5021..5695
                     /locus_tag="ACUQR0_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HHV9109018.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5711..8191
                     /locus_tag="ACUQR0_001963"
                     /pseudo
     CDS             5711..8191
                     /locus_tag="ACUQR0_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8207..9240
                     /locus_tag="ACUQR0_001964"
                     /pseudo
     CDS             8207..9240
                     /locus_tag="ACUQR0_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9322..9660)
                     /gene="rcnB"
                     /locus_tag="ACUQR0_001965"
     CDS             complement(9322..9660)
                     /gene="rcnB"
                     /locus_tag="ACUQR0_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HHV9109019.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9879..10727)
                     /gene="rcnA"
                     /locus_tag="ACUQR0_001966"
                     /pseudo
     CDS             complement(9879..10727)
                     /gene="rcnA"
                     /locus_tag="ACUQR0_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10849..11121
                     /gene="rcnR"
                     /locus_tag="ACUQR0_001967"
     CDS             10849..11121
                     /gene="rcnR"
                     /locus_tag="ACUQR0_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HHV9109020.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11213..11309
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11344..12132
                     /gene="thiM"
                     /locus_tag="ACUQR0_001968"
     CDS             11344..12132
                     /gene="thiM"
                     /locus_tag="ACUQR0_001968"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HHV9109021.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12129..12929
                     /gene="thiD"
                     /locus_tag="ACUQR0_001969"
     CDS             12129..12929
                     /gene="thiD"
                     /locus_tag="ACUQR0_001969"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HHV9109022.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            12994..13811
                     /locus_tag="ACUQR0_001970"
                     /pseudo
     CDS             12994..13811
                     /locus_tag="ACUQR0_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            13863..14609
                     /locus_tag="ACUQR0_001971"
     CDS             13863..14609
                     /locus_tag="ACUQR0_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHV9109023.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14583..15548)
                     /locus_tag="ACUQR0_001972"
     CDS             complement(14583..15548)
                     /locus_tag="ACUQR0_001972"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HHV9109024.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15545..16548)
                     /locus_tag="ACUQR0_001973"
                     /pseudo
     CDS             complement(15545..16548)
                     /locus_tag="ACUQR0_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16545..17822)
                     /locus_tag="ACUQR0_001974"
     CDS             complement(16545..17822)
                     /locus_tag="ACUQR0_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HHV9109025.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18079..19131
                     /gene="fbaB"
                     /locus_tag="ACUQR0_001975"
     CDS             18079..19131
                     /gene="fbaB"
                     /locus_tag="ACUQR0_001975"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HHV9109026.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19439..20293
                     /gene="gatY"
                     /locus_tag="ACUQR0_001976"
     CDS             19439..20293
                     /gene="gatY"
                     /locus_tag="ACUQR0_001976"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HHV9109027.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20322..21584
                     /gene="gatZ"
                     /locus_tag="ACUQR0_001977"
     CDS             20322..21584
                     /gene="gatZ"
                     /locus_tag="ACUQR0_001977"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HHV9109028.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21594..22046
                     /gene="gatA"
                     /locus_tag="ACUQR0_001978"
     CDS             21594..22046
                     /gene="gatA"
                     /locus_tag="ACUQR0_001978"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HHV9109029.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22077..22361
                     /gene="gatB"
                     /locus_tag="ACUQR0_001979"
     CDS             22077..22361
                     /gene="gatB"
                     /locus_tag="ACUQR0_001979"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HHV9109030.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22365..23720
                     /locus_tag="ACUQR0_001980"
     CDS             22365..23720
                     /locus_tag="ACUQR0_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HHV9109031.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23768..24808
                     /gene="gatD"
                     /locus_tag="ACUQR0_001981"
     CDS             23768..24808
                     /gene="gatD"
                     /locus_tag="ACUQR0_001981"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HHV9109032.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24914..25687
                     /locus_tag="ACUQR0_001982"
     CDS             24914..25687
                     /locus_tag="ACUQR0_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHV9109033.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25769..26668)
                     /gene="yegS"
                     /locus_tag="ACUQR0_001983"
     CDS             complement(25769..26668)
                     /gene="yegS"
                     /locus_tag="ACUQR0_001983"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HHV9109034.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27074..27391
                     /gene="yegR"
                     /locus_tag="ACUQR0_001984"
     CDS             27074..27391
                     /gene="yegR"
                     /locus_tag="ACUQR0_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HHV9109035.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27733..29094)
                     /gene="yegQ"
                     /locus_tag="ACUQR0_001985"
     CDS             complement(27733..29094)
                     /gene="yegQ"
                     /locus_tag="ACUQR0_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HHV9109036.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29241..29572)
                     /locus_tag="ACUQR0_001986"
                     /pseudo
     CDS             complement(29241..29572)
                     /locus_tag="ACUQR0_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7697 a         6869 c         7477 g         7570 t
ORIGIN      
        1 cagcttcagt tccgcttttt ccgccgccgc agcatctgaa aaacttttta tctgactttg
       61 ccctttggtg ccaacttttc cccaactgat atgcaactcg tttccctgtt gctccaccgt
      121 ccagaattta tgtgattttt cgtcctgata gataaagtgt ctcattctgc atccctgtaa
      181 ttttatccag ttgaatgcag atgctaccag tatttatacg ggttagagag agacaaaatc
      241 gcaagaaaac aactttagga atttataaag aaaaggcgct gtcgatgcag cgccttgaag
      301 ggggattatt tcacctgatg acccggttta gcaccggcat ccgggcttag caggaaaata
      361 tctttcccgc caggaccggc agccatcacc atgccttcag agataccgaa gcgcatttta
      421 cgtggtgcca ggttagccac cataatggtg tgacgaccaa tcagtgcctg cggatccggg
      481 taagcagaac gaataccgga gaagacattg cgtttttcac cgccgagatc cagcgtcagg
      541 cgcagcagtt tgtcagaacc ttcaacaaac tctgcgtttt cgatcagcgc cacgcgcagg
      601 tcaactttgg cgaagtcgtc aaaggtgatg gtttcctgaa tcggatcatc tgccagtggg
      661 ccagtaactg gcgcggtagt ggctttcact tcttctttag aggcttccac cagagcttca
      721 acctgtttca tatcgatgcg gttatacagc gccttgaacg gattcacttt gtggcccagc
      781 agcggttgct ggataccatc ccaggtcagt tccgtattga ggaaggcttc tgcacgctcg
      841 gtcagtttcg gcagtaccgg cttcaggtaa gtcatcagca cgcggaacag gttgatgccc
      901 attgagcaaa tcgcctgcag gtcagcatcg cgaccttcct gttttgcgac tacccacgga
      961 gcctgctcgt cgacatagcg gttagccagg tcagccagtg ccatgatttc gcgcactgct
     1021 ttaccaaatt cacggctttc ccacgcttca ccaatcactt cagcggcatc ggtaaaggtt
     1081 ttgtacagct cagggtcagc cagctcgctt gccagcacac cgtcaaaacg cttgttgata
     1141 aagcccgcat tacgggaggc caggttaacc actttgttca cgatatcggc attcacacgc
     1201 tgaacgaaat cttccaggtt gagatcgata tcatcaatgc gcgaagagag tttcgcagtg
     1261 tagtagtaac gcaggctgtc agcgtcaaaa tgattcagcc aggtgctggc tttaataaag
     1321 gtgccgcgag acttggacat cttcgcgccg ttcaccgtca catagccatg aacaaacagg
     1381 ttggtcggct tgcggaagtt gctgccttcc agcatggcag gccagaacag gctgtggaag
     1441 taaacgatat ctttaccgat gaagtggtac agctcggcgg tggagtcttt cttccagtat
     1501 tcatcgaagc ttacgctgtc gccgcgcttg tcgcacagat tcttgaaaga acccatgtag
     1561 ccaatcggtg cgtccagcca gacgtagaaa tatttgcccg gtgcgttcgg aatttcaaaa
     1621 ccaaagtatg gcgcgtcgcg ggagatatcc cactgttgca ggccggattc aaaccactcc
     1681 tgcattttgt ttgccacctg ctcctgcaac gcaccgctgc gggtccatgc ctgcagcatt
     1741 tcgctgaaag agggcagatc aaagaagaag tgttcagaat cacgcatcac tggtgtagca
     1801 ccagaaacca ccgatttcgg ctcgatcaat tcagtcgggc tgtaggtcgc gccgcagact
     1861 tcgcagttat cgccgtattg atccggggat ttacatttcg ggcaggtgcc tttcacaaaa
     1921 cggtccggca ggaacatgcc tttttccgga tcgtacagct gagagatggt gcggttttta
     1981 ataaaaccgt tttctttcag gcgagagtag ataagttctg acaactggcg gttctcttcg
     2041 ctgtgcgtcg agtgatagtt gtcatagctg atgttaaagc ctgcgaaatc agtctgatgc
     2101 tcctgactca tttcgccaat catctgctcc ggggtgatac cgagctgctg agctttcagc
     2161 atgatcggtg taccgtgggc atcgtcggcg cagatgaagt tgacctcgtg gccgcgcatt
     2221 cgctggtaac ggacccagac atcagcctgg atgtgctcca gcatatggcc gaggtggatt
     2281 gagccgttag cgtacggcag tgcgcacgtc accagaattt tcttcgcgac ttgagtcata
     2341 gtaggcatta cttcttaata gtgaaaaggg gttagatagt accaaatggg aaaatgttaa
     2401 gtaagtactt ctctatattc ttcaggtaaa ctatcagctg atggtttatc tatacaaaaa
     2461 aggagtcggg atgaacgaac aatcccaggc caaatcacca gaagccttgc gcgcaatggt
     2521 cgccgggaca ctggccaatt ttcagcaccc aaccctgaag cataacctca ccacgctgaa
     2581 agcgttgcac catgtcgcct ggatggacga cacgttgcat gtggaactgg ttatgccgtt
     2641 tgtctggcat agcgcgttcg aagagttaaa agagcaatgt agcgccgaac tgctgcgcat
     2701 caccggggcg aaagctatcg actggaaact gtcgcataac atagccactc tgaaacgcgt
     2761 gaagaatcag ccgggaatta acggtgtgaa gaacattatc gcggtgagtt ccggtaaagg
     2821 cggcgtgggt aaatcgtcca cggcggtaaa cctggcgctg gcactggcgg cggaaggggc
     2881 gaaagtcggc attctggacg ccgatatcta cggcccatca attcccacca tgctgggcgc
     2941 agaaaatcag cgtccaactt cgccggacgg tacccacatg gcaccgatta tgtctcatgg
     3001 cctggcaacc aattcaattg gttatctggt cactgacgac aatgcgatgg tgtggcgtgg
     3061 accgatggcg agcaaggcgt taatgcagat gttgcaggaa accttgtggc cggatctcga
     3121 ctatctggtg cttgatatgc cgccaggcac cggtgacatt caattgacgc tggcgcaaaa
     3181 cattccagta actggtgcag tcgtggtcac caccccgcag gacatcgcgt tgatcgatgc
     3241 gaaaaaaggc attgtgatgt tcgagaaagt cgaggttccg gtgctcggta ttgtcgagaa
     3301 catgagtgtg catatttgca gtaactgtgg tcaccatgaa ccgattttcg gtaccggtgg
     3361 tgcagagaaa ctggctgaaa aataccatac acagctgctg gggcagatgc cgttacacat
     3421 ctccttacgt gaggatctcg ataaaggcac gccaacggtt atcagccgtc cggagagcga
     3481 atttaccgct atctaccgcc agctggctga ccgcgttgca gctcagctct actggcaggg
     3541 tgaagtcatt cctggcgaga tttccttccg cgcggtctaa cgcctattaa ccattctggt
     3601 tattcttcat acaccgcaga gatgttatat tgatgcggtg tatttgtttt tgccgcatta
     3661 atgaacactt atttaatact gcgaatattc tgcggacctt cgtccgaaac tctacaaagt
     3721 cgagtgatcg tacaggcaat gcttcattat catccttcgt gctgtgtagt ggatgtacag
     3781 ctatagtttg ttatgcactc attctatcca ggcataacct ttactacagg gataacgttc
     3841 aatgaataag tattggctgt caggaattat ttttcttgcc tatggattgg cttctcccgc
     3901 agtctcttca gagactgcca cattaacgat taatggcagg atcagtcccc caacatgcag
     3961 tatggctatg gttaatagtc aaccacaaca gcattgtggt cagttaacgt ataatgttga
     4021 cacccgtcat caggtttctt cgcccgtcaa aggtgttacg acagaagtgg ttgttgcagg
     4081 tagcgatagc aaacgtcgaa tcgtgttaaa tcgctacgat taatttggca acctgatttt
     4141 aattgcattt agaaagacga aatcatttat ataaatgatt tcgtcttttt tattgatatc
     4201 aacaatacca tttacataaa tctacattaa ttaatagatg tgataaatat ttaatattct
     4261 taagtatttg ttgtatttat attgcattgg ttaatttgtt ttaaatcaat aaaacaatat
     4321 gtttacattg cggcagcttt tttgatgtga gaaatttgtt tccaagagat acagactctt
     4381 aacaaccaaa aataaatttt attaaggaac atattatgaa acgttcaatt attgctgccg
     4441 ctgtcttttc ttcttttttt atgagcgctg gagtatttgc tgcagacgtt gataccggaa
     4501 cattaaccat taaaggaaat attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt
     4561 cagtaagtat taatatgccg actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt
     4621 ctacctatga tgatgcagtc ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag
     4681 aagttgctgg tgtaaaactc tcgttgatca caaacgacaa aataaccggt aacgataagg
     4741 cgatagccag tagcaacgat accgtaggtg ataacagcga tgtcctagat gtttctgcac
     4801 cttttaacat tgagagttat aaaacagcgg aaggtcaata tgctattccg tttaaagcaa
     4861 aatacctgaa actgacagat aactcagtgc aatcaggtga tgtgttatct tctctggtta
     4921 tgcgtgtggc gcaggattaa taaataaaat tgcaagtttc tccaggtaat aaacatggct
     4981 gctatcccca tggcggcttt ttaatttaag aggcattaag atgaaaggat tattatcttt
     5041 actcattttt tctatggtcc ttcctgcaca tgccggaatt gttatctacg ggacgcgcat
     5101 tatttacccg gcagaaaata aagaagttat ggtgcagttg atgaaccagg gaaatcgttc
     5161 ttcgctgctg caggcgtgga ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca
     5221 ggttcctttc atgttaacgc caccagtggc aaaaataggg gcaaattccg ggcagcaagt
     5281 aaaaatcaaa attatgccga ataaactgcc cactaataaa gaaagcattt tttatctgaa
     5341 tgttctgtat attccaccaa atagtccaga gcaagaaggc aagaatgcac tgaagtttgc
     5401 gatgcaaaac agaattaagt tgttttaccg gccagcgggt attgctccgg taaataaagc
     5461 gacctttaaa aagttactgg taaatcgcag tggcaatgga ttggtgataa aaaatgactc
     5521 agctaattgg gtgacgattt cggatgtcaa agctaataat gttaaagtca attatgaaac
     5581 tattatgatt gcccccttag aaagtcagag tgttaatatc aaaagtaata atgcaaataa
     5641 ctggtatctg accattatca atgaccacgg caactatatt agtgacaaaa tttaattgca
     5701 ggagctgcct atgttgagaa tgaccccact tgcatcagca atcgtagcgt tattgctcgg
     5761 cattgaagct tatgcagctg aagaaacctt tgatacccat tttatgatag gtggaatgaa
     5821 agaccagcag gttgcaaata ttcgtcttga tgataatcaa cccttaccgg ggcagtatga
     5881 catcgatatt tatgtcaata agcaatggcg cgggaaatat gagattattg ttaaagacaa
     5941 cccgcaagaa acatgtttat caagagaagt tatcaagcgg ttaggcatta atagcgataa
     6001 cttcgccagc ggtaagcaat gtttaacatt tgagcaactt gttcagggtg ggagctatag
     6061 ctgggatatc ggggtttttc gtctcgattt cagtgtcccg caggcttggg tggaagaact
     6121 ggaaagtggc tatgttccac cggaaaactg ggagcggggt attaatgcgt tttatacttc
     6181 ttattatgtg agtcagtatt acagcgacta taaagcgtcg ggtaacaaca agagtacata
     6241 tgtacgtttt aacagcgggt taaatttact ggagtggcaa ctgcattctg atgccagttt
     6301 cagtaaaaca aataacaatc caggggtgtg gaaaagcaat accctgtatc tggaacgtgg
     6361 atttgcccaa tttctcggca cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt
     6421 tgattctgtt cgcttcagcg gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc
     6481 gaaacaaaat tttacgccac gggtgcaggg gattgctcag agtaacgcgc tggtaactat
     6541 tgaacagaat ggttttgtgg tttatcagaa agaggttcct cctggcccgt ttgcgattac
     6601 agatttgcag ttggccggtg gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg
     6661 ctcggtaacc acctatctgg tgccttatgc agcggtgcca aatatgctgc aacccggcgt
     6721 gtcgaaatat gattttgcgg cgggccgtag ccatattgaa ggggcgagca agcaaagtga
     6781 ttttgtccag gcaggttatc agtatggttt taataactta ttgacgctgt atggcggcac
     6841 gatggttgct aataattact atgcgtttac cctctgaacg ggttggaaca cgcgcattgg
     6901 cgcaatttcc gtcgatgcca cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga
     6961 cgggcaaagt tatcaaattg cctacaacaa atttgtgagc caaacgtcga cgcgttttgg
     7021 tctggcggcc tggcgttatt cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc
     7081 aaacaataaa gataattatc gccgtgatga aaacgatatc tatgacattg ccgattatta
     7141 ccagaacgat tttggccgta aaaatagctt ttctgccaat atgagccagt cattgccaga
     7201 aggctggggt tctgtgtcct taagtacgtt atggcgagat tactgggggc gtagcggcag
     7261 cagtaaggat tatcagttga gttattccaa taactggcgg cggataagct ataccctcgc
     7321 ggcaagccag gcgtatggcg agaatcatca tgaagagaaa cgttttaata tttttatatc
     7381 aattccctgt gattggggtg atgacgttac gacgcctcgt cggcaaatat atatgtctaa
     7441 ctcaacgacg tttgatgatc agggttttgc ctcaaataat acgggattat caggaacagt
     7501 agggagtcgg gatcagttca attatggtgt caacctgagt catcaacatc agggaaatga
     7561 aacgacagct ggggcgaatt tgacctggaa cgcgccggtt gcgacagtga atggcagtta
     7621 tagtcagtcg agtacttatc gacagactgg agccagtgtt tcagggggca ttgtcgcctg
     7681 gtcgggtggc gttaatctgg cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc
     7741 aggaattaaa gatgcttatg tcaatgggca aaaatatcgc acaacaaacc gtaatggagt
     7801 ggtggtatac gacggaatga caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca
     7861 aagcgatagc gaagcagaat tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc
     7921 ggttgtactg gttaattttg ataccgatca gcgcaagccc tggtttataa aagcgttaag
     7981 agcggatggt caaccattaa cgtttggtta tgaagtcaat gatatccatg gtcataatat
     8041 tggcgttgtc ggccagggaa gccagttatt tattcgcacc aatgaaatac cgccatcggt
     8101 taatgtagca attgataagc aacaaggact ttcatgcaca atcaccttcg gtaaagagat
     8161 tgatgaaagt agaaattata tttgccagta atagtcaggt ggttctatgg aaattcgcat
     8221 aatgctattt atattaatga tgatggttat gcctgtgagc tatgcggcat gttatagtga
     8281 gttatctgtt cagcacaatt tggttgttca ggggaatttt gcacttactc aaacacaaat
     8341 agcgacatat gagcataatt ttaatgattc gtcatgcgtg agtacaaata ctatcacccc
     8401 tatgagccca tcggatatta ttgttggact ttataacgat accattaaat taaatttaca
     8461 ttttgaatgg accaataaaa acaacatcac gttgtcaaat aatcagagca gtttcaccag
     8521 tggttattcg gtaacggtga cacctgcggc cagtaatgca aaagtgaata tttctgcggg
     8581 gggcggtggt tcagtgatga ttaatggtgt tgcgacatta tccagtgctt catcatcgac
     8641 acgcgggagt gccgcagtac aatttctact gtgtttatta ggtggcaagt catggggtgc
     8701 atgtgtaaat agctacagaa atgcattggc acaaaatgca ggtgtctatt ccttcaatct
     8761 gacattgtca tacaacccga taaccacaac ctgcaaacag gacgatttat taattacttt
     8821 agacagtatt cccgtttcac aattaccagc cacaggtaac aaagcaacaa taaatagtaa
     8881 aaaaagggat attattctgc gttgtaaaaa tttattaggt caacaaaatc aaacatcacg
     8941 gaaaatgcag gtgtatttat caagttctga cttgttaacc aacagcaaca caatactgaa
     9001 aggtgcggaa gataatgcgt aggatttatt cttgaaagta atggttcgcc agtcacactt
     9061 ttaaatatca ctaacagcag taaaggatac acaaatttaa aagaaattgc ggcaaagtca
     9121 aaacttacag atacaacggt ttcaattccg ataacagcca gttactacgt ctacgataca
     9181 aacaaagtta aatctggcgc actggaggca accgcattaa tcaacgtgaa atacgactaa
     9241 taacaagcaa aacgagcaag tggctaataa taaaaaataa caaggtgtct gcatttcccc
     9301 ctcatgatga gggggctttt tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat
     9361 gattttaccg tcggtgtcgc tgatcaacac gtacgcgcca cccatatagg tccagtgcgt
     9421 cccggcatca ggcgcgggca ggttacgtaa ctgccactgc ttaatgttgt actcatcggt
     9481 gcggtacatc tctgggacgg tatcacctgg tttgaaatgc ttaaagtcag caatgaaact
     9541 acttaactca tacttagaaa ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc
     9601 ccaggcggca ctggtaacca gcaaaagcac acccagcaac atcttatttt taatggtcat
     9661 aaactctcca ctttggggat ctgtaatatc agtcgcatat ctttcctgtc atacaatata
     9721 actgacaagc gatattttgt tagtgatccc atgatttcgt aaaaagtgta aatgaaagga
     9781 tgccctaaaa gcatccttag cgagcagaga gtcagcggaa atagctgatt taagaatcat
     9841 cctgggctga agataacatg caagcctaaa ggattgagtt atcgcattat gcccatgaag
     9901 ccatgtacgc ccatatacac accgactaag ccaatcaaca gactggaaaa atagggggcg
     9961 cgtttagcga gagtgttaaa tccgctccag cgttttgcga cctgctgaac gctgattgct
    10021 gcgccaacgc ctacggtgac aagcgttaac gccaggccaa tgctgaaact gacgaccagt
    10081 gttgcgccca gtgtcagggc tttcaactga atgcatatca acagcacggt aattgctgcc
    10141 gggcagggga taaagccacc ggttaagcca aataacaaaa tttgccagtt gatgacctct
    10201 ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc gtgcatgggc atcctgatac
    10261 tcgccatatt cgtgatggtg atgatgtcct tggtcgtggt gatgttcgtg gtcttgatga
    10321 tgttcgtgat cgtgatgatg gtgatgatgc tcataatcat gcccgtgcat attctccagc
    10381 cagttgcgtt cgccgcgcta ggtacgccag aacatccaga acgcggtgcc aataatgatc
    10441 actgcggaaa tcagctggag ccacggttct gctgattgag cagtaaagcg cttactgatc
    10501 accatcccgc caaaggcaat caaccagacc accgcggtat gcgaaatggt tgctgccagt
    10561 ccgagcatca ccgcctgttt aatggtgcct ttgatggcga tgataaacgc cgccatcatc
    10621 gtttttgagt gccctggttc caggccatga agcgcaccaa gtaagatggc gctggggatg
    10681 aagaaccagg cgtttccttg ctgaagaaga gttgtaaatt cggtcatgat aataattctt
    10741 agtattaatt cggcaatctg attctactcc cccccagtac ctgatactac ccccccagta
    10801 gattaatagt gctatgattt ttcatattct tgttaaccag gtgttgccat gtctcataca
    10861 atccgtgata aacagaaact gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg
    10921 ctcaagaaaa tgctcgacga gccgcacgaa tgcgctgcag ttttacaaca gattgctgct
    10981 atccgtggag cggtaaacgg tctgatgcgg gaagtgatta aaggtcatct gacggaacac
    11041 atcgttcacc agggggatga gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg
    11101 ctggattcat atatcaaata atttattaac gcgattgtaa aactgccgtt tttcctcgtt
    11161 tacaacgggg gcgctggaca ttaccatcct cctctgcgat ttatcatcgc aaccaaacga
    11221 ctcggggtgc ccttctgcgt gaaggctgag aaatacccgt atcacctgat ctggataatg
    11281 ccagcgtagg gaagtcacgg accaccaggt cattgcttct tcacgttatg gcaggagcaa
    11341 actatgcaag tcgacctgct gagttcagcg caatctgcgc acgcgttaca cctttttcac
    11401 caacattccc ctcttgtgca ctgcatgacc aatgatgtgg tgcaaacctt taccgccaat
    11461 accttgctgg cgctcggtgc atcgccagcg atggttatcg aaaccgaaga ggccagtcag
    11521 tttgcggcta tcgccagtgc cttgttgatt aacgttggca cactgacgca gccacgcgca
    11581 caggcgatgc gtgctgccgt tgagcaagca aaaagctctc aaacacgctg gacgcttgat
    11641 ccagtagcgg ttggtgcgct cgattatcgc cgccattttt gtcatgaact tttatctttt
    11701 aaaccggcag cgatacgtgg taatgcttcg gaaatcatgg cattagctgg cattgctaat
    11761 ggcggacggg gagtggatac cactgacgcc gcagctaacg cgatacccgc tgcacaaaca
    11821 ctggcacggg aaactggcgc aatcgtcgtg gtcactggcg aggtggatta tgttaccgat
    11881 ggacatcgta tcattggtat tcacggtggc gatccgttaa tgaccaaagt ggtaggaact
    11941 ggctgtgcat tatcggcggt tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa
    12001 aatgtcgcat ctgcctgtca ctggatgaaa caagccggag aacgcgcagt cgccagaagc
    12061 gaggggccag gcagttttgt tccacatttc cttgatgcgc tctggcaatt gacgcaggag
    12121 gtgcaggcat gaaacgaatt aacgctctga cgattgccgg tactgatccg agtggtggtg
    12181 cggggattca ggccgatctt aaaaccttct cggcacttgg cgcttatggt tgctcagtta
    12241 ttactgcact ggtggcgcaa aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg
    12301 attttgtcgc cgcccagctc gattcggtgt tcagcgatgt gcgaatcgat accactaaaa
    12361 tcggtatgtt ggcggaaacc gatattgttg aagcggtggc agaacggttg caacgttatc
    12421 agatccaaaa cgtggtactc gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt
    12481 caccttcggc ggttgctacg ctgcgcagtc gattattgcc acaggtttca ttaataacgc
    12541 caaacttgcc cgaagctgcc gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa
    12601 tgctggaaca agggcgatcg ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg
    12661 gtcatctgga tgatgagcaa agcccggact ggctgtttac ccgcgagggt gaacaacggt
    12721 ttaccgcacc gcgcattatg accaaaaaca cccacggcac tggttgtaca ctctctgcgg
    12781 cgttggctgc actacgcccg cgccatacaa actgggctga caccgtacag gaggcaaaaa
    12841 tctggctttc atcggcgtta gcccaggccg acacgctgga agttggtcac ggtattggtc
    12901 cggttcacca cttccacgcc tggtggtgag caccgtacta tagctgcttt ggttattgtc
    12961 agcctttggc actgacattc agggatgaaa ccaatgcagc taagaattac cagtcgaaaa
    13021 aaactcaccg ctctattatg cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt
    13081 cagacggtga attttttcta ttcgacagca gttcagatta cagactacat ccacttctac
    13141 ggttatcgcc cggttaaatc tttcgctatt cgcattcctg ccagttacac cattcacgga
    13201 ttagatgttt cgcgctggca ggagcgtatc gactggcagc gggtggcaaa aatgcgcgac
    13261 aacgatatcc gcttacagtt tgcttttatt aaggctacgg aaggcgaaaa gctggtggac
    13321 ccctattttt cgcgtaactg gcaattaagc cgcgaaaatg gcctgctgcg cggggcgtat
    13381 cattatttct ccccgtcggt atccgcttca gttcaggcgc gattatttct gcaaacggtg
    13441 gatttctcac aaggcgattt ccctgccgtg ctggacgtag acgaacgggg aaaattatcg
    13501 gcaaaagaat tacgcaagcg ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga
    13561 aaaaagccga ttatttactc aggagccgtt ttttatcaca ccaatctggc gggctatttc
    13621 aatgaatatc cgtggtgggt ggctcactat tatcaacgtc gtccggacaa tgacggcatg
    13681 gcctggcgct tctggcagca ttccgaccgt ggacaggtag atggcatcaa tggtccggtg
    13741 gattttaatg tgtttaatgg cacggggatg agttgcaggc attcgttgat gggattaaag
    13801 aaacgcctta aataagaagc agtgatatac tcccaccaga ctccctggca ggaaaaagac
    13861 aaatggaaca agcgcatacc cagctgattg cccaactgaa cgagcgtatt ttagcggcgg
    13921 ataacacgcc gctttatatt aagtttgccg aaacggtaaa aaatgccgtg cgcagcgggg
    13981 tgctggagca tggcaatatt ttgcccggtg agcgtgattt aagtcagtta accggcgttt
    14041 cgcgcattac ggtgcgcaag gcgatgcagg cgctggaaga agagggcgtg gtgacgcgtt
    14101 cgcgcggtta cggcacgcag atcaacaaca tcttcgaata ctcgttgaaa gaagcgagag
    14161 gtttttctca gcaggtggta ttgtgcggga aaaagcccga tacgctatgg gttaataagc
    14221 gtgtcgtgaa atgtcccgaa gaagttgcgc agcagctggc ggtcgaagcg ggaagtgatg
    14281 tctttttgct taagcgtatt cgctatgtcg atgaagaagc ggtatcgatt gaggaatcgt
    14341 gggttccggc gcatttaatt catgatgttg atgccatcgg aatttctctt tatgactatt
    14401 tccgcagcca gcacatttac ccacaacgta cgcgttcccg cgttagcgcc cggatgccgg
    14461 atgccgagtt tcagtcacat attcagttag atagcaaaat accggtgctg gtgatcaagc
    14521 aagtggcgct tgaccaacag caacggccta ttgagtacag cattagccac tgtcgcagcg
    14581 atctatacgt ttttgtgtgc gaggagtagt tcctcgcgcg ttggcgcaca atcaccgccc
    14641 cgatgcccga caacccacga cgccactgca ttgcccagta atacggcatc cgccagtggc
    14701 agaccagagg ccagcccggc aagtacgcca cctgcatgac tgtcgcccgc cccaatggtg
    14761 tctacaactt gcgttggaaa tgccggaatg cagccagaag cgtcgttgct gaaataccat
    14821 gcgccttctt tatcgagacg aacgatcaac ggcgcggcaa atttctcctg ccattgcttg
    14881 ccaagtgttg ttatctctgc ggataaagca aaacgttcgg cggcaatctc agcctcttga
    14941 cgattgagtg acactaaagg tcgacaggcc atgatccgcg ccagtaatgc gtccgggata
    15001 tcgccaatac gtgggcaaaa atcgataaac ggcgtcacgt cttgcagctt ttccagccat
    15061 tccactaaca attcgccgca gggcgaggcc agttgataac cggaaaaata gagcaggttg
    15121 ccaggcgcaa cggttaatcg tgccaaccat tggcgattcc attgattttc cacaccgctg
    15181 aatgacataa aagtgcgttc gccatccggc tcaaccagtg ccagacacca gccgttatcg
    15241 ctttcggcgt tatcgatcag gctgattaac ccctctttcg ccatccggtt gcgaataatc
    15301 tccgcccaca cgccctgacc gagcggcaag gcattacccg cttcgatgcc gaggcgcttt
    15361 aacgccacgg caatattcag tgcgcagccg ccaacgttaa cgctctgctg tttcagttcg
    15421 atatcgcacc cacgccaggg gagggcataa gcgtcggcga tcacgtcaat gaccgccgcg
    15481 ccgacgacca tcaccggctg acgcgtggtt aattcaggca gcagtgtgtg taatcgagcg
    15541 ccgctcatac cgcctcccgt tgttgacgat atttcgccag cgctgtggca tagcggttga
    15601 aatcaagctg atttaccgca tccagctccg cctttaacgc aggatcgaca gcgttaatgc
    15661 catgcaacgc gccgcaaatc gccgtcgcca tagcacctat ggtgtctgtg tcgccgccaa
    15721 gattggcgca caggacggcg cagcggtttg ggtcggtttg tgccagttca actagcgcaa
    15781 tggcgcacgg aacggactca atagtgctgg tgcctgcgcc aacgacctgg taaagctgtt
    15841 cgctggcgga ttcggtgccg tcggcattac gcacaatttt cagcgcaatc tccagacgtg
    15901 ccgccagtga ggcgctgaac gtggtggtgc gtttttgctg tgcatgtcgc gcaattgaag
    15961 gcaatgaatc aacaatcgct gaccagcgtt ctccgtcaat ggcacgagaa atcgcccatg
    16021 cgatgactac cgcgcctgca accgccagat cggatttatg ggtcgggctg gacgccagcg
    16081 ccacatcatc aataaaggaa tcaacatcac gcgctggaag caaacaacct aatggcgaga
    16141 cgcgcatcgc tgcgccgttg gttacgccgt tgttttccag ttcagcaacg ggtttaccgt
    16201 cgcgaatggc gttaagcgca atcttcgagg tcggacctag tacgtttttg ttaaaggcgt
    16261 cgaaacgcaa cgcccagtcg agaatattac gcccaatcag attcggatcg atcttgcctt
    16321 cacattccag taacgcatcc gccagacaca ggccatcgag gtatcgtcgg tgaattcggc
    16381 gcggttaaaa taacaggccg cgttattctc ctttggtcca ggcagaaaac ggtcaatcca
    16441 gccaaagtgt gctttaacgc ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc
    16501 ccctaacgcc tgcccataaa gagcaccgag aatacgttct gttttcattt aacttcccct
    16561 tgtgtcaacg caatatctcg atcatcgacc ttgatagccg taatttcgtt gtcggattcg
    16621 cggaaaaaaa tcatgaacag cacggcgata atggcaatca tcaccgcgcc gaaagtccac
    16681 atcccggacc agttgaaagt cagtccgttt accggttcct gataagcgaa catcttttcc
    16741 atcatcacac cgccaagacg atagccgagc aaactgccga agccctggca gcagagcgtg
    16801 atcagcccct gcgcagcggt acgcatatgc acgggggctt ttttatcgac atagatgtaa
    16861 gcggtaacgt agtaaaaatc gtaacttacg ccgtgaagca aaataccgag gaacagtaac
    16921 gcgtaggtga aatattcatc cgcactaccg taaataaaga agccatagca gatcgcagcg
    16981 gtgaccagac caagcaataa tacctttttg ataccaaagc gtttagtgaa aaacggcaat
    17041 gccagcataa agaagatttc agagaactgg ccgagcgtca tccagccggt ggcgtttttc
    17101 atgccaactt cggtcagata accgttggca aagatgtaat agaacgccag tggcatcgca
    17161 aacaggaatg aacagaagaa aaagacgagg aagtttttat cgcgcagcag gatcagcgca
    17221 tccaggccga gcatgacttt aatgtccatt ttgccggtgc tttttggcgg cgtgtcgggc
    17281 aggaaaaacg caaacacacc gagcagagca gaacttccgg cggtaatcag cagcgggatg
    17341 ttagtcggtg agatatcggc ataccccagc atttgcggca agaaaccaca tgccagaccg
    17401 gaggcaatcc agccgatagt gcccatcaca cgaatgcgtg ggaaatcacg ctccacatcc
    17461 ggcacgttgg caaaagcgat gctgttagtc agcgcaatgg tcggcatata ggttagcgag
    17521 taggccagca gtaacggaaa gaacccggca aaagtggtct gttgcgcagc gaaatacatc
    17581 agcacagcac cagcgaacat caataccgcc agcactttct gcgccgagaa aaagcggtca
    17641 gtgatggagc caaccagaat tggcgacagg atcgccgcaa tggctgtgca ggcatacgac
    17701 cagccaattt ctccggcact aaaaccgctt ttacttaacc acaaccacaa tggcacaaac
    17761 cacgcgcccc agataaacca ttcaacaaac atcatgaacg acagctttgc tgttgttttc
    17821 atcttttaat tccttcatga caggtaatgt aaggtacgcg atgacaatac cacataataa
    17881 tgccttttga atacctttgc ggtgaaaact tgaccgcgct aacatttttt ctgatgaatc
    17941 gagccaacag aaaacgctga aaaaacatcc aaaagatgga aaaactcgcc cgtaacgact
    18001 aggattatga ttgcccgcaa aaggcgggcc aggacaatcc atagccgata tccaatcgga
    18061 atttacggga gcatagtaat gacagatatt gcgcagttgc ttggcaaaga cgccgacaac
    18121 cttttacagc accgttgtat gactattcct tctgaccagc tttatctccc cggacatgac
    18181 tacgtagacc gcgtgatgat tgacaataat cgcccgccag cggtgttacg taatatgcag
    18241 acgttgtaca acaccgggcg tctggctggc acaggatatc tttctattct gccggttgac
    18301 cagggcgttg agcactctgc cggagcttca tttgctgcta acccgctcta ctttgacccg
    18361 aaaaacattg ttgaactggc gatcgaagcg ggctgtaact gtgtggcatc aacttacggc
    18421 gtgttggtgt cggtatcgcg gcgctatgcg catcgcattc catttctcgt caaacttaat
    18481 cacaacgaga cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc cagcgtggag
    18541 caggccttca acatgggcgc ggtggcggtt ggtgcgacta tctattttgg ctcggaagag
    18601 tcacgtcgcc agattgaaga aatttctgcg gcctttgaac gcgcgcacga gctgggcatg
    18661 gtgacagtgc tgtgggccta tttgcgaaac tccgccttta agaaagatgg cgttgattac
    18721 catgtttccg ccgacctgac tggtcaggcg aaccatctgg cggcaaccat aggtgcagat
    18781 atcgtcaaac aaaaaatggc ggaaaataac ggcggctata aagcgattaa ttacggttat
    18841 accgacgatc gcgtgtacag caagttaacc agcgaaaacc cgattgatct ggtgcgttat
    18901 cagttagcta actgctatat gggccgggct gggttgataa actccggcgg tgctgcaggc
    18961 ggtgaaactg acctcagcga tgcagtgcgt actgcggtta tcaacaaacg cgcaggcgga
    19021 atggggctga ttcttggacg taaggcgttc aagaaatcga tggctgacgg cgtgaaactg
    19081 attaatgccg tgcaggacgt ttatctcgat agcaaaatta ctattgcctg atacgcctca
    19141 tacgaacagc accaccccgt aggacggata agacgtttac actgcatccg gcaacctatg
    19201 cctgatgcga cgcggaagcg tcttatcatg cctacatagc actgccacgt atgtttacac
    19261 cgcatccggc ataaaaacac gcgcactttg ctacggcttc cctatcggga ggccgttttt
    19321 ttgcctttca ctcctcgaac aattttcata ttgttgtttt tgtgatcatt atctcgacat
    19381 ttaaaagcaa ataatttcat tatattttga aatcgaaagc aaacgacaag atatgaaaat
    19441 gtacgtggta tcgacaaagc agatgctgaa caacgcacag cgcggcggtt atgcggttcc
    19501 ggcattcaat attcacaatc tcgaaacgat gcaagtggtg gtggaaaccg ctgccaacct
    19561 gcatgcgccg gtcatcatcg ccggaacgcc tggcacattt actcatgctg gtacagaaaa
    19621 tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac catccactgg caattcatct
    19681 cgaccatcac acgaaatttg acgatatcgc tcagaaggtt cgttctggcg tgcgctcagt
    19741 catgattgac gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca aagaggtggt
    19801 ggatttttgc catcgctttg atgtcagcgt cgaggcggag ctggggcaac ttggcggcca
    19861 ggaagatgat gtgcaagtca atgaagtcga tgcgttgtac accaaccccg cacaggcgcg
    19921 tgaatttgcc gaggcaaccg ggattgattc cctggcggtc gccatcggca cggctcacgg
    19981 tatgtatgcc agcgcaccgg tgcttgattt ttctcgactg gagaacattc gccagtgggt
    20041 gaacttaccg ctggtgctgc atggcgcgtc aggattatcg actaaggata ttcagcaaac
    20101 catcaaactg gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg ccttcttgca
    20161 gtcgttaaaa aattacctga ccgagcaccc cgaagcgacc gatccccggg attatttgca
    20221 gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg attgccgatt gtggctgcga
    20281 gggcagggca taacgcacct gccatttaac aaggaaaaaa catgaaaacg ttaattgccc
    20341 ggcataaagc tggtgaacat atcggcatat gttcagtctg ttctgcccat ccgttggtta
    20401 tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa agtgctgatt gaagcaacgt
    20461 caaaccaggt caatcaattt ggcggttata ccggaatgac accggcagac tttcgcgaat
    20521 ttgtttttac gattgccgat aaagttgggt ttgcacgcga acgcattatt ctcggcggcg
    20581 atcatctggg accaaactgc tggcagcaag aaaatgcgga tgcggcgatg gaaaaatccg
    20641 tcgagctggt aaaagcatat gttcgtgctg gcttcagtaa aattcatctt gatgcgtcaa
    20701 tgtcctgcgc gggggatacc ataccgttag caccagaaac ggttgcggaa cgagctgctg
    20761 tgctttgctt tgctgcggaa agtgtggcga cagattgcca gcgtgagcaa ctgagctatg
    20821 tcattggcac tgaagttccg gttccgggcg gtgaggccag cgccattcag tcagtacaca
    20881 tcacccatgt tgaagatgcc gccaatactt tacgtacgca tcaaaaggcc tttattgccc
    20941 gtgggctgac agaggcgtta acacgtgtga ttgccatcgt ggtgcagccg ggtgtggaat
    21001 ttgatcacag caatattatc cattatcagc cgcaggaagc gcaggcgctg gcgcaatgga
    21061 tagaaaacac ccgaatggtt tatgaagcac attctaccga ttaccagacc cggacggctt
    21121 attgggaatt agtccgcgat cactttgcaa tattgaaagt cggtcccgca ttaacctttg
    21181 ctttacgcga ggcgatattt gcactggcac aaattgagca ggaacttatc gcccccgaaa
    21241 atcgcagcgg ttgcctggcg gtaattgaag aagtgatgct cgacgaaccg caatactgga
    21301 aaaaatatta tcgcacgggt tttaacgatt cattactgga tattcgttac agcctgtcgg
    21361 atcgtattcg ttattactgg ccgcatagtc ggattaaaaa tagcgttgaa acgatgatgg
    21421 tgaatctgca aggcgtggac atcccactgg gcatgattag tcagtatctt cccaaacaat
    21481 ttgaacgcat tcagtccggg gaattatcag caatgccgca tcagcttatt atgaataaaa
    21541 tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg tatatgacta
    21601 acctgtttgt tcgtagcgga atttcttttg tcgatcgtag cgaagtttta acctatatcg
    21661 gtaatgagat gctcgcgaaa ggtgtggttc atgatacctg gccacaggca ttaattgcca
    21721 gagaagcaga attccctacc gggataatgc ttgagcagca cgccattgca ataccgcatt
    21781 gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt aaggccaaca aataaagttc
    21841 attttcagca agcggatgat gataacgacg tggcggtatc gttggttatt gcgttgattg
    21901 tggaaaatcc gcagcagcaa ttgaaacttt tacgctgttt atttggcaag ttacaacagc
    21961 ccgatatcgt cgagacacta attactcttc ctgaaactca gttaaaggaa tacttcacaa
    22021 agtatgtttt agattcagac gaataaatcc ctctgtaaca ataaaaagta ctatttatga
    22081 aacgcaagat tattgtcgct tgcggaggcg cggttgcgac ctctacgatg gcggcggaag
    22141 aaattaaaga gttgtgtcag agtcataata ttcctgttga attaatccag tgtcgggtta
    22201 atgaaataga aacctatatg gatggtgtgc atttgatatg taccactgcc agggtggatc
    22261 gtagttttgg cgatattccg ttagttcacg gcatgccttt tgtttctggt gtcggtatcg
    22321 aagcattaca aaataaaatt ctgactatct tacaggggtg acctatgttt tcagaagtca
    22381 tgcgttatat tctcgacctc ggccctacag tgatgctgcc ggttgtcatc attatttttt
    22441 ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc gggtctgcat atcgggattg
    22501 gctttgttgg cattggcctt gtgattggct taatgctgga ttccattggt ccggcggcga
    22561 aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc tggccgggct
    22621 cttcaccaat gacctgggcg tcgcaaattg cgctggtggc gattccgatt gcgatattgg
    22681 ttaacgtggc gatgttactg acccgtatga cgcgggtggt aaatgttgat atctggaata
    22741 tctggcatat gaccttcacc ggcgcgttgc tgcacctggc aaccggttca tggatgatag
    22801 ggatggcggg tgtggtaatt cacgcggcgt ttgtttataa gctctgcgac tggtttgccc
    22861 gcgatacccg aaatttcttt gagctggaag gtattgctat tccgcacggt acgtcggcgt
    22921 atatggggcc gattgcggtg ctggtcgatg ctatcatcga gaaaatccca ggcgttaacc
    22981 gaattaaatt tagcgccgac gatattcagc gcaaatttgg cccttttggc gaacctgtca
    23041 ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc gggttacgat gtcaaaggtg
    23101 tattgcagct ggcggtaaaa acggcggcag ttatgctgct gatgccacgg atgattaaac
    23161 ccatcatgga tggtttaacg cccatcgcta agcaggcccg tagtcgttta caggcgaagt
    23221 tcggcggtca ggagttcctg attggcctgg atcccgcatt actgctgggg catacggcgg
    23281 tggtatcggc aagcctgatt tttattccgc tcaccatttt aattgctgtt tgtgttccgg
    23341 gtaatcaggt actgccgttt ggcgatcttg ccactatcgg cttcttcgtg gcgatggcgg
    23401 tcgcggtgca tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc attatgagca
    23461 tcaccctgtg gatcgcgacg caaactattg gtttgcacac ccaaccggcg gctaatgctg
    23521 gggcgttaaa agccgggggt atggtggctt caatggatca gggcggttct cccattacct
    23581 ggttactgat tcaggttttc tccccgcaaa atattcccgg tttcattatt atcggcgcaa
    23641 tttatctgac cggtattttc atgacctggc gtagagcgcg tggttttact aaacaagaga
    23701 aagccgttct cgcagaataa tttttacctg agggggaggt tatccccctt aacaatcagg
    23761 agtttttatg aaatcagtag tgaatgatac tgatggtatc gtgcgcgttg caaaaagcgt
    23821 cattcctgaa attaaacatc aggatgaggt gcgggtaaaa attgccagct cgggcttatg
    23881 tggttccgat ttacccagga tatttaaaaa tggtgcacat tattatccaa taacgttagg
    23941 ccatgaattt agcggctata ttgatgcggt gggatccggt gttgatgatt tacaccctgg
    24001 cgatgcggtt gcctgtgtgc cgttattacc ctgttttact tgtccagagt gtttgaaagg
    24061 gttttattcc cagtgcgcaa aatatgattt tattggctcg cggcgtgatg gtggatttgc
    24121 tgaatatatt gtcgttaagc gaaaaaatgt ctttgctcta cccacggata tgcctattga
    24181 ggatggggct tttattgagc cgattaccgt tggcctgcat gcttttcatt tagcgcaagg
    24241 ttgtgagaat aaaaacgtta ttattattgg tgccggaacc attggcctgc tggctattca
    24301 gtgcgctgtc gcgctgggag caaagagtgt gacggcgatc gacattagct cagaaaaact
    24361 ggcactggca aaatctttcg gtgcgatgca aacatttaac agtagcgaaa tgagcgcgcc
    24421 gcaaatgcag agcgttttac gcgaactgcg ctttaatcag cttatcctcg agacggctgg
    24481 cgtaccgcaa actgtcgaac tggcggtaga gattgccggt cctcatgccc aactggcgct
    24541 ggtgggcacg ttgcatcagg atctgcattt aacatcggca acgtttggca aaatattgcg
    24601 taaagagctg acggttatcg gcagttggat gaactattcc agcccttggc cggggcagga
    24661 gtgggaaacg gcgagccggt tgctgacaga acgtaagtta agcctggagc cattaatcgc
    24721 tcaccgtgga agctttgaaa gcttcaccca ggcggtgcgt gacatcgctc gtaatgccat
    24781 gccgggcaaa gtgttgctca ttccatgaaa ccgcgggcca gcgtgatgct ggcccggtat
    24841 tgtgcaaaac agatcattca ccaatggtcc cccttcgttt acactagcca caattgaata
    24901 tgggtaaatg acgatgaatt cattcgagcg aaggaataag atcatccaat tagtgaatga
    24961 acagggaacc gtgcttgttc aggatctggc gggagtattt gctgcctcgg aagcgacaat
    25021 ccgtgccgat ttgcgctttc tcgaacaaaa aggcgtggtt acgcgctttc atggcggtgc
    25081 ggcgaaaata atgtccggta atagtgaaac cgagacccag gaagtcgggt ttaaagagcg
    25141 atttcagctc gccagcgcgc caaaaaacag aatagcgcag gcggcagtca aaatgatcca
    25201 tgaagggatg accgttattc tcgacagcgg aagtaccaca atgcttatcg ccgaaggatt
    25261 aatgaccgct aaaaatatca cggtgattac caacagtctc ccggcagcgt ttgccctttc
    25321 cgaaaataaa gacattactc tggtcgtctg tggtggcacg gtccgccata aaacgcgctc
    25381 gatgcatggt tccattgccg agcgttcatt gcaggatatt aatgccgatt taatgtttgt
    25441 cggtgctgat ggtattgatg cggttaatgg tattacgacc tttaatgaag gttattcgat
    25501 tagcggggcg atggtcaccg ccgcaaataa agtgattgct gttctcgatt catcgaaatt
    25561 taatcgtcgc ggttttaatc aggtactgcc aatcgaaaaa attgacatta ttgttaccga
    25621 tgatgctgtg tcagaggtgg ataaattggc attgcagaag acacgggtaa aattaattac
    25681 ggtgtagtat tgttctgtat gccggataag gtgtttacac cgcatccggc attacggatt
    25741 ctccctggtt caagagagaa tagtttgatt aacgcaataa tggacaatct ggtggtaatc
    25801 gacaacgcaa cgccgccgga agtatttcaa tatggaagtt ttgcccactc aacggttcgc
    25861 catcaagatt aaaagtgatt tcgtgtgggg cttgtatatc aaaccacgac gaagcgcctt
    25921 cgataatatt cgggttatct tcgtcagatt ttaaggttga tacgagagcc ggaagaattt
    25981 catcgccggt aaaaatgcgc agttgcagca agccatcgtt aattaacgcg ttcgggcaca
    26041 attgttgacc gccaccggcc tgacgcccgt taccaatacc aatgaccagg gcgtcacctt
    26101 gccagtgaaa gttttcaccg cggatttcac aacggtccgg ttgcagagta tccatgcgca
    26161 ttaagccatg aatgatgtaa gagacgccac ccagcgcggc ttttaatttt tccggcgttt
    26221 ctgtggtaat acgcgtccca aatccgcctg tcgccatatt aataaaacag gtttgtttgt
    26281 tgacctgcgc catatctatc gcaatggcat taccggcaat tgccagtttc agcgccttat
    26341 ccagtgcctc aggaatccct acactggtgg caaaatcatt ggcggttcct aatggcaaaa
    26401 ttcccagcgc gggtatgtca tccccctcac actgaatcaa cgccgtagaa acttcattaa
    26461 tggtgccatc gccaccaccg gcaatcaccg ttgcgacgcc caacttccgg gcctcctcta
    26521 caaatcgtgc ggcatcacct ttctcccagg tgacccgcac atggatcgtc attccttcct
    26581 cacgcaacag cataattgct tcgcgcaagg gtagattgtc agtactttta ccattaagaa
    26641 tcagtaagct ggcgggaaat tctgccatga tcgtatgtgc ctttatgatt ggtctgtaga
    26701 tagtgtagag cagaaaacaa aaggtggagt cagaacagga tgaaagtcgg aggataaaaa
    26761 gtcagttcaa gtatggagga ttcagtggtt accaccagga gcttgcaatg gggacagcga
    26821 atactaacgg ctgccaccga taaatttcaa aaaagagcat atacctaata ttcaactaaa
    26881 cagtggcatc ttcaatataa tatattaaag cccccatgga gttaccctga agggcctcaa
    26941 tgtccgtaat tcctacttat gtaggaaatg ttgtacagag catttattat aatcctattc
    27001 aattataata atcatgccat tattatattt aaacactaga gagtgtcgtt ggtatttaat
    27061 gggggaaggt gagatgaaaa agatagctgc tatatcatta attagtattt ttcttatatc
    27121 tggttgtgct gtgcataatg atgaaacaag tatcggtaaa tttggtcttg catataaaag
    27181 taatattcag cgtaaactcg ataaccaata ctacaccgaa gccgaagctt ctttagccag
    27241 gggcagaata tctggtgcag aaaatatagt aaaaaatgat gcagcccatt tctgtgttac
    27301 tcagggcaaa aaaatgcaga tagttgacct gaagacagaa ggtgcaggat tacatggcgt
    27361 cgctcgtctg acattcaaat gtggagagtg agaatatttt tttgtaagcg tcaaacatgc
    27421 gcgttctggc tgtgcgtagc cggaacctgg gcgagcacga taccgttacg tgaaaggcat
    27481 tgtgctatga agggggattc tatcgatgtg gtcaatggaa gacggttatc agggatcaat
    27541 ccttctttac agcttatgta caaaaaataa gcccgtgtaa gggagattac acaggctaag
    27601 gaggtggttc ctggtacagc tagcatttta tggattatgt ttttcagcga aacggatgat
    27661 aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc cgggaaaaat
    27721 aatcgaaatt aatcacttac cgtgggggtt acgcgtggtt tccccggaga aattacgcat
    27781 tagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag tataaccatc
    27841 gcctggtgcg accggcatag cttcgccttt ggcgttttcc atgtgctcaa gggtaaagtt
    27901 aatgttgcct tgcggcgtca tcagctcaag actgtcgcca acggagaatt tatttttcac
    27961 cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa actgctggcg
    28021 gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat gacgacgcag
    28081 gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca ggctggtatc
    28141 gaacggtttt cccgcagcgg cgtcatcgat agctttgcgg taaacctgtg cggtgcgtgc
    28201 gcaatagtag aaagatttgg tacgaccttc gattttcagc gaatgcacgc ccattttggt
    28261 caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt aagtgccgtg
    28321 ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga tcataaacac
    28381 tttgtcggtt ggcgcgccga tacccagcgt cggctcaaca ttttgcaccg gaatcggctc
    28441 gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt tgtactccca
    28501 gcggcaggcg ttggtgcagg tgccctggtt cgggtcgcgc ttgttgatat agccagagag
    28561 caggcagcga ccggagtagg ccatgcacag tgcgccgtga acgaagatct cgatctccat
    28621 atccggcacc tgattatgga tctcttcaat ctcttccagc gacagctcgc gagagaggat
    28681 cacgcgggtc agtcccattt gctgccagaa tttcaccgtc gcccagttca cggcgttagc
    28741 ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca taatcagccc
    28801 tggatcggac ataatcagcg catccggccc catttccacc accggtttca ggtcacggat
    28861 aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa actttttccc
    28921 cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt cgttgttgcg
    28981 cacacgtagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat aagcgaaagc
    29041 gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa acataattct
    29101 ctcgttctga tgacaggtca gatccgcttc acctgatgaa gcggttaggg gaggccccca
    29161 ctttaagggc gggcattgta gcgctgtagg ggggatctgg taaagcgccg gacgccaaca
    29221 acccacgccc ggcatcgtaa ttacgtattg tctttcaccg tctggcttgt gccattggct
    29281 ttaacggagg caattccggt ttcacgagat tgcgcggtgg cgtacatctg gctggagccg
    29341 ataatttgat gattagcggc tttcagattg aaatagaatt tgccgttgct tgccgttttt
    29401 tctcatagcg ctcttcttgc gggctgttgc tacgcaccga cgcgatgccc ttttccgcag
    29461 aggcttttga ggtatacagc tcgctggtga ggatagtctc accattgccc gcttttagca
    29521 caaaccggaa ctgattatca ctgctcttac tgagttcaaa ccaaccagcc ataatgactc
    29581 cttgattgaa attgcttatc gaaaacagca tct
//
LOCUS       DBKELA010000045        29234 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.045, whole genome shotgun sequence.
ACCESSION   DBKELA010000045 DBKELA010000000
VERSION     DBKELA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.045"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..216
                     /locus_tag="ACUQR0_001987"
     CDS             <1..216
                     /locus_tag="ACUQR0_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9109037.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(258..1334)
                     /gene="glpQ"
                     /locus_tag="ACUQR0_001988"
     CDS             complement(258..1334)
                     /gene="glpQ"
                     /locus_tag="ACUQR0_001988"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HHV9109038.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1339..2697)
                     /gene="glpT"
                     /locus_tag="ACUQR0_001989"
     CDS             complement(1339..2697)
                     /gene="glpT"
                     /locus_tag="ACUQR0_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HHV9109039.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2970..4598
                     /gene="glpA"
                     /locus_tag="ACUQR0_001990"
     CDS             2970..4598
                     /gene="glpA"
                     /locus_tag="ACUQR0_001990"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HHV9109040.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4588..5847
                     /gene="glpB"
                     /locus_tag="ACUQR0_001991"
     CDS             4588..5847
                     /gene="glpB"
                     /locus_tag="ACUQR0_001991"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HHV9109041.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5844..7034
                     /gene="glpC"
                     /locus_tag="ACUQR0_001992"
     CDS             5844..7034
                     /gene="glpC"
                     /locus_tag="ACUQR0_001992"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HHV9109042.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7227..8177
                     /locus_tag="ACUQR0_001993"
     CDS             7227..8177
                     /locus_tag="ACUQR0_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HHV9109043.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8218..8679)
                     /locus_tag="ACUQR0_001994"
                     /pseudo
     CDS             complement(8218..8679)
                     /locus_tag="ACUQR0_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(8743..9945)
                     /locus_tag="ACUQR0_001995"
     CDS             complement(8743..9945)
                     /locus_tag="ACUQR0_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HHV9109044.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10032..10574)
                     /locus_tag="ACUQR0_001996"
     CDS             complement(10032..10574)
                     /locus_tag="ACUQR0_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HHV9109045.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10853..11278
                     /gene="nudI"
                     /locus_tag="ACUQR0_001997"
     CDS             10853..11278
                     /gene="nudI"
                     /locus_tag="ACUQR0_001997"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HHV9109046.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11317..11919)
                     /gene="ais"
                     /locus_tag="ACUQR0_001998"
     CDS             complement(11317..11919)
                     /gene="ais"
                     /locus_tag="ACUQR0_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HHV9109047.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12227..13366
                     /gene="arnB"
                     /locus_tag="ACUQR0_001999"
     CDS             12227..13366
                     /gene="arnB"
                     /locus_tag="ACUQR0_001999"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HHV9109048.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13370..14338
                     /gene="arnC"
                     /locus_tag="ACUQR0_002000"
     CDS             13370..14338
                     /gene="arnC"
                     /locus_tag="ACUQR0_002000"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HHV9109049.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14338..16320
                     /gene="arnA"
                     /locus_tag="ACUQR0_002001"
     CDS             14338..16320
                     /gene="arnA"
                     /locus_tag="ACUQR0_002001"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HHV9109050.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16317..17207
                     /gene="arnD"
                     /locus_tag="ACUQR0_002002"
     CDS             16317..17207
                     /gene="arnD"
                     /locus_tag="ACUQR0_002002"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HHV9109051.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17207..18859
                     /gene="arnT"
                     /locus_tag="ACUQR0_002003"
     CDS             17207..18859
                     /gene="arnT"
                     /locus_tag="ACUQR0_002003"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HHV9109052.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18856..19191
                     /gene="arnE"
                     /locus_tag="ACUQR0_002004"
     CDS             18856..19191
                     /gene="arnE"
                     /locus_tag="ACUQR0_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HHV9109053.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19191..19577
                     /gene="arnF"
                     /locus_tag="ACUQR0_002005"
     CDS             19191..19577
                     /gene="arnF"
                     /locus_tag="ACUQR0_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HHV9109054.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19571..19838)
                     /gene="pmrD"
                     /locus_tag="ACUQR0_002006"
                     /pseudo
     CDS             complement(19571..19838)
                     /gene="pmrD"
                     /locus_tag="ACUQR0_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
     gene            complement(19948..21303)
                     /gene="menE"
                     /locus_tag="ACUQR0_002007"
     CDS             complement(19948..21303)
                     /gene="menE"
                     /locus_tag="ACUQR0_002007"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HHV9109055.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21300..22262)
                     /gene="menC"
                     /locus_tag="ACUQR0_002008"
     CDS             complement(21300..22262)
                     /gene="menC"
                     /locus_tag="ACUQR0_002008"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HHV9109056.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22262..23119)
                     /gene="menB"
                     /locus_tag="ACUQR0_002009"
     CDS             complement(22262..23119)
                     /gene="menB"
                     /locus_tag="ACUQR0_002009"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HHV9109057.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23134..23892)
                     /gene="menH"
                     /locus_tag="ACUQR0_002010"
     CDS             complement(23134..23892)
                     /gene="menH"
                     /locus_tag="ACUQR0_002010"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HHV9109058.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23889..25559)
                     /gene="menD"
                     /locus_tag="ACUQR0_002011"
     CDS             complement(23889..25559)
                     /gene="menD"
                     /locus_tag="ACUQR0_002011"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HHV9109059.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25648..26943)
                     /gene="menF"
                     /locus_tag="ACUQR0_002012"
     CDS             complement(25648..26943)
                     /gene="menF"
                     /locus_tag="ACUQR0_002012"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HHV9109060.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27022..27327)
                     /gene="elaB"
                     /locus_tag="ACUQR0_002013"
     CDS             complement(27022..27327)
                     /gene="elaB"
                     /locus_tag="ACUQR0_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HHV9109061.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27382..27843)
                     /locus_tag="ACUQR0_002014"
     CDS             complement(27382..27843)
                     /locus_tag="ACUQR0_002014"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9109062.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27908..28825
                     /gene="rbn"
                     /locus_tag="ACUQR0_002015"
     CDS             27908..28825
                     /gene="rbn"
                     /locus_tag="ACUQR0_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HHV9109063.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29016..29204
                     /locus_tag="ACUQR0_002016"
                     /pseudo
     CDS             29016..29204
                     /locus_tag="ACUQR0_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7086 a         7856 c         7299 g         6993 t
ORIGIN      
        1 gatacagcga tgaattttat tcgacaggga ttaggcatcg ccttacaacc agagttaacg
       61 ctgaaaagca ttgcaggtga attgtgttcc gttcctcacg aaccaacctt ctatcgacag
      121 atttcgttgc tgactaaaga aaagccggta gaaggcagtc cactgttttt actacaaatg
      181 tgcatggaac aattagtggc gattggaaaa atttgatatg agacaggatg gcgcatgaac
      241 gccatcctgc agtaacatta ctctttatta agaaatttta ctgccttatc agggaaatca
      301 gtaaacagcc catttacacc cgctttgtta tacagagcat catataactg attcacatca
      361 ggagtgtatt caggcagttt atctgaccgc acggtataag gatgcactac cagtttattc
      421 tgctgggcat cttgcaccat gccagtgagt ttgatattac ccggctgcga tgtctcctca
      481 atcaacatat ggtaatccgg accaatacca tctgcatatt ccgccacctg tttcatggct
      541 cccggcttaa acatccagtc gtagttgtaa ttaacccagc ttccatccgg ctgtttctgc
      601 tgcgtttcat tccagtcggt ataggcaatc agctgtacca gattgagctc catgcccatt
      661 ttgggttcca gctcattctt aatacgcttc agctcatcag catcaaaaca ttgcaaataa
      721 actttatcgt ctttaccggt gtaaccatat ttcttcagca cttccagcgt ttttgccgca
      781 atatccttcc cttcctgatg atggaaccac ggcgctttga tttctggata gataccgata
      841 tttttcccgg tagagtgatt taacccctga acaaattcaa tctcttcttc aaaggtgtgc
      901 acccggaagt cggacttacc cattgggaaa cgccccggat aagtctgtac ttttttaccg
      961 ttttcaatat cgaaaccttc ggtaaatttc agcgacttaa tttcatccag cgtgaaatct
     1021 atcgcgtagt aacgaccgtc tttgcgcgcc cgatccggga aacgatcggc aacatcagta
     1081 acacgatcga gataatggtc atgcagaaca accagatggt cgtctttggt catcaccaaa
     1141 tcctgttcca gataatccgc tccctgcgca tacgccatcg cttttgctgg cagcgtatgc
     1201 tccggcaaat atccactggc accgcgatgg gcgatgacta ttttttcgtt gctgtccgcc
     1261 gccattgcac tgcttcccat gactatagtg ctcatcatga tcgccatgct aaggtttttc
     1321 agcttcaatt tcatgccatt agcctccgtt gcgtttttgc agtaattgtt catggcgacg
     1381 tttttcgcca atcatcacaa caatcaacaa gataaccgcc agaatgctgc cgccaatcat
     1441 taccataaag ccgccatccc agccgaagaa gtccacggtg tagccaacaa tcgcgctcgc
     1501 cgccaccgaa ccgcccaggt aaccaaacag cccggtaaag cccgctgccg tacctgccgc
     1561 ttttttcggt gccagttcca gcgcatgcag accgatcagc atcacaggac cgtagatcag
     1621 gaagccgata acaatcatac aaatcatatc gacggttggg ttacctgccg ggttcatcca
     1681 gtaaacgata gtcgcgatgg tcaccagtgt cataaagaag acgccggttg ccccacggtt
     1741 accacggaag actttgtccg acatccagcc gcacagcagg gtgcccggaa tacccgcata
     1801 ctcatagaag aagtaggccc aggaggattt atccagcgcg aaatgcttaa cctctttcag
     1861 ataagtcggt gaccagtcga ggatgccgta acgcagcaga taaacgaaca cgttggcgat
     1921 ggcgatgtac cacagcagtt tgttcggcag cacgtactgc atgaagattt gcttcgcggt
     1981 cagctcctgt tccgcttttt cgttatagtc gtccggataa tcatttttgt actcttcgat
     2041 cggcggcaag ccacaggatt gcggggtatc gcgcatcatc gcaaaggcga ataatgccac
     2101 cagaatggcg cagaaagcag gcatatagag cgccgcatgc cagtcattga accaggccat
     2161 ccccagcagg aacagcagcg gcggaatacc accaccgacg ttgtgcgcac agttccacac
     2221 tgacacaatg ccgccacgtt ctttctgcga ccaccagtgc accatagtac gaccacacgg
     2281 cggccacccc atcccctgga accaaccgca gaggaacaac agtacaaaca tcaccgcaat
     2341 gctcgacgtc gcccatggca caaagcccat aaacaacatc actgccgccg ccagaatcaa
     2401 acctgcgggc aggaaaacgc gcggattcga gcgatccgat accgaaccca tgatgaattt
     2461 cgaaaatcca taagcaatcg agatccccga aagggcaaaa cctaaatcac cgcgtgagaa
     2521 tccctgctca accagataag gcatagcaag cgcaaagttc ttacgaacca aatagtaagc
     2581 cgcatagcca aagaatatcc ccaggaaaat ttgccagcgc aatcgacgat aagtcggatc
     2641 gatctccgcg gcaggtaagc gcgctttgtg tggcgctggt ttaaaaatac tcaacattga
     2701 tagcctccgt ggcccgtggt cttatttatg attaacagcc tgattcagtg agagaacctg
     2761 ccgtttcttg agttgccgcg atgttaagaa aacattcata aattaaatgt gaattgccgc
     2821 acacattatt aaataagatt tacaaaatgt tcaaaatgac gcatgaaatc acgttttact
     2881 ttcgaatcat gagcgaatat gcgcgaaatc aaacaattca tgtttttact atggctaaat
     2941 ggtaaaaaac gaacttcaga gggataacaa tgaaaactcg cgactcgcaa tcaagtgacg
     3001 tgattatcat tggcggcggc gcaacgggag ccgggattgc ccgcgactgt gccctgcgcg
     3061 ggctgcgcgt gattttggtt gagcgccacg acatcgcaac cggtgccacc gggcgtaacc
     3121 acggcctgct gcacagcggt gcgcgctatg cagtaaccga tgcggaatcg gcccgcgaat
     3181 gcattagtga aaaccagatc ctgaaacgca ttgcacgtca ctgcgttgaa ccaaccaacg
     3241 gcctgtttat caccctgccg gaagatgacc tctcattcca ggccactttt attcgcgcct
     3301 gcgaagaagc agggatcagc gcagaagcta tagacccgca gcaagcgcgc attatcgaac
     3361 ctgccgttaa cccggcactg attggcgcgg tgaaagttcc ggatggcacc gttgatccat
     3421 ttcgtctgac cgcagcaaac atgctggatg ccaaagaaca cggtgccgtt atccttaccg
     3481 ctcatgaagt cacggggctg attcgtgaag gcgcgacggt gtgcggtgtt cgtgtacgta
     3541 accatctcac cggcgaaact caggcccttc atgcacctgt cgtggttaat gccgctggga
     3601 tctgggggca acacattgcc gaatatgccg atctgcgcat tcgcatgttc ccggcgaaag
     3661 gatcgctgct gatcatggat caccgcatta accagcatgt gatcaaccgc tgccgtaaac
     3721 cttccgacgc cgatattctg gtgcctggcg ataccatttc gctgattggt accacctctt
     3781 tacgtattga ttacaacgag attgacgata atcgagtgac ggcagaagag gttgatattc
     3841 tgctgcgtga aggggaaaaa ctggcccccg tgatggcgaa aacgcgcatt ttgcgggcct
     3901 attctggcgt gcgcccgctg gttgccagcg atgacgaccc gagcggacgt aacgtcagcc
     3961 gtggcatcgt gctgctcgac catgctgaac gcgatggtct ggacggattt atcaccatca
     4021 ccggtggcaa actgatgacc tatcggctga tggctgaatg ggctaccgac gcggtatgcc
     4081 gcaaactggg caacacgcgc ccctgtacga ctgccgatct ggcactgcct ggttcacaag
     4141 aacccgctga agttaccttg cgtaaagtca tctccctgcc tgccccgctg cgcggttctg
     4201 cggtttatcg tcatggcgat cgcacgcctg cctggctgag cgaaggccgt ctacaccgta
     4261 gcctggtatg tgagtgcgaa gcggtaactg cgggtgaagt gcagtacgcg gtagaaaatt
     4321 taaacgttaa tagcctgctg gatttacgcc gtcgtacccg tgtggggatg ggcacctgcc
     4381 agggcgaact ctgcgcttgc cgcgctgccg gactgctgca acggtttaac gtcacgacgt
     4441 ccgcgcaatc tatcgagcaa ctttccacct tccttaacga acgctggaaa ggcgtgcaac
     4501 ccatcgcctg gggagacgca ctgcgcgaaa gcgaatttac ccgctgggtt tatcagggac
     4561 tgtgtggtct ggagaaggag cagaaagatg cgctttgata ctgtcattat gggcggtggc
     4621 ctcgccggat tactctgtgg cctgcaactg caaaaacacg gcctgcgctg tgccattgtc
     4681 acgcgtggtc aaagcgcgtt gcatttctca tccggttcgc tggatttgct gagccatctg
     4741 ccagatggtc aacccgtggc agacattcac agtggactgg aatctttgcg tcagcaggca
     4801 cctgcacatc cttactctct tctcgggcca caacgcgtgc tcgatctcgc ttgccaggcg
     4861 caggcattaa tcgctgaaag cggtgcgcaa ttgcagggca gcgtagaact ggctcaccag
     4921 cggattacgc cgctcggcac tttgcgtgca acctggttaa gttcgccaga agttcccgtc
     4981 tggccgctac ccgcgaagaa gatatgtgta gtggggataa gcgggctgat ggatttccag
     5041 gcgcatcttg cggcggcttc gttgcgcgaa ctggatcttt cggttgaaac cgcagaaata
     5101 gagctgccgg aactggatgt cctgcgcaat aacgccaccg aatttcgcgc ggtgaatatc
     5161 gcccgtttcc ttgataatga agaaaactgg ccgctgttac ttgatgcgct tattcctgtc
     5221 gccaatacct gcgaaatgat cctgatgccc gcctgcttcg gcctggccga tgacaaactg
     5281 tggcgttggt tgaatgaaaa actaccttgt tcactgatgc ttttgccaac gctgccgcct
     5341 tccgtgctgg gcattcgtct gcaaaaccag ttacagcgcc agtttgtgcg ccaaggtgga
     5401 gtgtggatgc cgggcgatga agtgaaaaaa gtgacctgta aaaatggcgt agtgaatgaa
     5461 atctggaccc gcaatcacgc cgatattccg ctacgtccac gtttcgcggt tctcgccagc
     5521 ggcagtttct ttagtggcgg actggtagcg gaacgtaacg gcattcgcga gccgattctc
     5581 ggccttgatg tgctacaaac cgccacgcgg ggtgaatggt ataagggcga tttttttgcg
     5641 ccgcaaccgt ggcagcagtt cggtgtaacc actgatgaga cgctacgccc gtcgcaggca
     5701 gggcaaacca ttgagaacct gtttgccatc ggttcggtgc tgggcggatt tgatcccatc
     5761 gcccagggat gcggcggcgg tgtttgtgcc gtcagtgctt tacatgccgc tcaacagatt
     5821 gcccaacgcg caggaggcca acaatgaacg acaccagctt cgaaaactgc attaagtgca
     5881 ccgtctgcac caccgcctgc ccggtgagcc gggtgaatcc cggttatcca gggccaaaac
     5941 aagccgggcc ggatggcgag cgtctgcgtt tgaaagatgg cgcactgtat gacgaggcgc
     6001 tgaaatattg catcaactgc aaacgttgtg aagtcgcctg cccgtccgat gtgaagattg
     6061 gcgatattat ccagcgcgcg cgggcgaaat atgacaccac gcgcccgtcg ctgcgtaatt
     6121 ttgtgttgag tcataccgac ctgatgggta gcgtttccac gccgttcgca ccaatcgtca
     6181 acaccgctac ctcgctgaaa ccggtgcggc agctgcttga tgcggcgtta aaaatcgatc
     6241 atcgtcgcac gctaccgaaa tactccttcg gcacgttccg tcgctggtat cgcagcatag
     6301 cggctcagca agcacaatat aaagaccagg tcgctttctt tcacggctgc ttcgttaact
     6361 acaaccatcc gcagttaggt aaagatttaa ttaaagtgct caacgcaatg ggtaccggtg
     6421 tacaactgct cagcaaagaa aaatgctgcg gcgtaccgct aatcgccaac ggctttaccg
     6481 ataaagcacg caaacaggca attacgaatg tagagtcgat ccgcgaagct gtgggagtaa
     6541 aaggcattcc ggtgattgcc acctcctcaa cctgtacatt tgccctgcgc gacgaatacc
     6601 cggaagtgct gaatgtcgac aacaaaggct tgcgcgatca tatcgaactg gcaacccgct
     6661 ggctgtggcg caagctggac gaaggcaaaa cgttaccgct gaaaccgctg ccgctgaaag
     6721 tggtttatca cactccgtgc catatggaaa aaatgggctg gacgctctac accctggagc
     6781 tgttgcgtaa catcccgggg cttgagttaa cggtgctgga ttcccagtgc tgcggtattg
     6841 cgggtactta cggtttcaaa aaagagaact accccacctc acaagccatc ggcgcaccac
     6901 tgttccgcca gatagaagaa agcggcgcag atctggtggt caccgactgc gaaacctgta
     6961 aatggcagat tgagatgtcc acaagtcttc gctgcgaaca tccgattacg ctactggccc
     7021 aggcgctggc ttaaactccc ttctgatgcc cggtaagcat gtggttaccg ggcatttttg
     7081 cgtacacgat tccgtgccca atgtatgcgt tgcaacgcag tgaaaattcc tctgaaaacg
     7141 tctcgcaaag gctgaaactg gcagatgtca aaggcctggg ataaccgtaa tgtcgcgtca
     7201 tcataaatat caggtgacgg acaaccatga ccgaatcaac aacctcctcc ccgcatgatg
     7261 cggtatttaa aacctttata ttcacacccg aaaccgcacg ggattttctc gaaatacatt
     7321 taccagaacc actgcgcaag ctttgcaacc tgcaaacctt acgcctggaa cccactagtt
     7381 ttattgaaaa aagtttacgc gcttactact cggatgtttt gtggtccgtg gaaaccagcg
     7441 acggtgacgg ctatatctac tgcgtgattg aacatcaaag ctctgcagaa aagaatatgg
     7501 cttttcggcc aatgcgctat gccactgccg ccatgcagag tcacctagat aaaggctatg
     7561 acagagttcc gctggtggtg ccattgctgt tttatcatgg cgaaacatcg ccctacccgt
     7621 actcacttaa ctggctggat gagtttgacg atccgcaact tgcccggcag ttgtacaccg
     7681 aagcttttcc gttggtggat atcaccatcg tacctgacga tgagatcatg caacatcggc
     7741 gtatagctct gctggaactg attcaaaagc atattcgcga ccacgattta atcggcatgg
     7801 tcgacaggat caccacgctt ttggttagag gcttcactaa tgacagccag ctacaaacac
     7861 tgtttaatta tctgctgcaa tgcggcgata cctcccgttt cacccgtttt attcaggaga
     7921 ttgccgaacg ttcaccacta caaaaggaga gattaatgac tattgctgaa cggctacggc
     7981 aggaaggaca tcaaattggc tggcaagaag gtaaattagt aggtttgcag caaggtaaat
     8041 tagaaggttt gcaggaaggt atgcatgaac aagccattaa aattgctttg cgcatgctgg
     8101 aacagggcat tgatcgtgac caagtgctcg cggccaccca gctaagcgaa gccgatctgg
     8161 cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc tttgatatca
     8221 ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca gtcgttgatc
     8281 cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg ctccccacgc
     8341 caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt tacccgcagc
     8401 acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc cggcgttatc
     8461 cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat caatcccttc
     8521 gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct gcactaacag
     8581 acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc gcgcagcccg
     8641 tgccacactg gccccaacac cacgctcacc gtagggaggg ttggacaaat tttgccggag
     8701 ggggaatgtg ccggtgcagt aaaccggcac agagtggcga aatcagacgg ataaagtcat
     8761 ggactcaaca acctcaatcc agccatgctc actagcgatg tcctggccgt ttaaccaacg
     8821 gcgtagcata ttcagtgcca tcattgcgca cacttcctga cggatagcca ggctgtagcg
     8881 tgtagtgctg aaacgcacac gcagagcgaa agtgccgtct ggcgtggcta gcgcaaagtt
     8941 gagatgctcg ttctcgaaac ccgaaacagc cagtgccagc ccggcaaaat ggttggcccg
     9001 ccgttctgta atccagtgcg cagtttgcgc cagggtttct tcctgtgaag gaatcacttc
     9061 acacgccagc aatggagcac ctgcgcgaga aagttgcaaa gccaataaac caccggtgaa
     9121 ttgctcgctc aacgtcaggc tgaactggcg gttttgcaat tcgcgactga tctgcgcggg
     9181 cagtccttcg gtgccttcga aaatcacgct ctgtccggca acacgtttaa cgtccagcca
     9241 cagtttttcc atcgcctgtt gctcgcttgc cggtccggtg agtttcagtt cgatgatcgg
     9301 cattgaggag cgatagccca ttgttacgcc cggcggcagt tgtagagtgt ccaggctttg
     9361 tgccagatcg ctttccgaac gaccaaaggt agttaaacgc agacaaaccg gcggctgtgg
     9421 taaagaaaaa cgctcgcgca ggcgcggcag gatttcgtgc tcgaccatca ccttaaattc
     9481 tgacggtacg ccgggagtaa agaacatcag gcaacgatta agctgcacgg caaaaccaca
     9541 ggcggtgcct accgggttat tgataaattc agcactggca ggcagctccg cttgtttacg
     9601 gttgctcggt gccattactc gtccacgttc gtggaaatag cgttccatct ctttgagcca
     9661 ggcttcatgc agcaccaggc cttcaccttt tgctgtcgca gcggcgagtg cgcttaaatc
     9721 atcgctggtc ggtcccagcc cgccgttaac gatcagcaca tcggcgtgct gactacgttc
     9781 gcgaagaatg gtgactaagt catcaaggtt atcccccacc gtattgcggc gagataatgg
     9841 cagcccctga tgaaagaaaa aatcggccag ccaggcagcg ttagtgtcaa cgatttgccc
     9901 gtataacact tcatccccgg tggatagcat ttccactttt aacatggtta tctcctgctt
     9961 ttgttatcga atcactataa cgcaaaggga gataaggcgt agtaagaaac tgaatgccgc
    10021 gccggaaggg atcagaaact ggcgttaacc ccaacataca ggccgtcagc cacagcgttg
    10081 tcgcggttac cgtctttacc cgacagattc aggtagcgat aacccgcctc aatactgact
    10141 ggacgcataa tggtgtaacg cgcgccagca ttcgcttcct cataactttg aataccgctg
    10201 gagagcgaat ccggagagta gtaatactcg ccaaacaaac ggaagctgtt gccaattttc
    10261 cactgcaaac cacctcctac cgctgcggca taaccttcat cgccgtaatt cgggttggtg
    10321 tacacgcctt ttccgccaac ggtcgccatt aacggcccga gaggcagatt caaccccagc
    10381 cccacgcccg cgacgtcacc gtcgtcgtcg ttatgtgtcc agttaccgct taaagccagg
    10441 cccgtcgatt cagtcccaaa accgacacca atattggtgt attctttacc cgcctggccg
    10501 ctgatgctca ttgcattgac cgatgcagaa accaacagca ttccggccag acctgctagc
    10561 gcaatttttt tcattatccc ctttcccaca agcaattaat caaaattccc aaagctgcgg
    10621 gattgtactg cctggcacag aggattcaat gagcgaaaaa agggatctag tcacttattg
    10681 taactaaata cttcttaaat aattaaacgc cgaaaaaata aacacacttt accgatacac
    10741 acaaaaataa taataagtgc agttatcatt gcaaaagaga ttactgtcac tttcacatat
    10801 ttctgagtta ccgtattctc atggtttctt tttatttata aggaataccc ctgtgcgaca
    10861 acggactatt gtatgccctt tgattcaaaa tgatggtgct tatttgctgt gcaaaatggc
    10921 cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg ggtggcggcg tggagtctgg
    10981 cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa gaactgggag aacagctgct
    11041 tttgacagaa atcacgccgt ggaccttcag cgatgatatt cgcaccaaga catatgcaga
    11101 tggtcgcaag gaagagattt atatgattta cctgattttt gactgcgttt ctgccaaccg
    11161 agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg gtaaaacctg aagatctggt
    11221 gcattatgat ttaaatgttg ccacccgaaa aacgttacgt ttgaaaggtc ttctttaaca
    11281 atgatgacag ccatcactgg ctgtcagatt gaaaatttag tggttaacga attccccatc
    11341 cagataaact ttgccttttt ccacatgcat gactaaacca tccagataat caggtttaaa
    11401 tatcgcatca cgcttatttt tagcaatata tgtcaggcaa tgattatggg tgaaaataac
    11461 gatattttta tcaggcgctt tgctttgtaa gtccttaatt gcactataaa tctcattacc
    11521 gcactgaaga agtcgtttat ctaccgtcag ctttttaccc gcggaaaacc aggtcgccga
    11581 ctgaatggtc cggacggtat tactggaata aagatcgaaa tcagggatgt cagcgctaaa
    11641 agcgttgccc agttcacggg catcctgggt acctttaacc gtaatacctg ttttatctga
    11701 caagcattga ttggttgaac gatcgcaacg ttcagcatgg cgaaacaaaa cgacaaccgg
    11761 gtgctgctgt gccagtctgg ccaatgtttt attgtcgata cgtggcaaac cattgctact
    11821 ccaggcggca tgagtaccca gtccggcaat tgcagcgagc gccagtaaaa tgatgaaata
    11881 tttttttgac ttcaatgatg agcggcaaaa agctaacacg taaactccac ctatagacaa
    11941 gcgcaaccag acaattaccg tgaaattgag cgacatttct ggcgataatt agcagttggt
    12001 gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa atttttgcca aatgcaaagc
    12061 ctaaataaga aaaaatataa aaatttcaat atttacgtct aatattagtt tcttaaggtt
    12121 aagttaatat tctatcctta aaatttcgct ccaaatggca aaatatacac aacactcttt
    12181 atagcaaata taagtggaca ggtattcaat ggcggaagga aaagcaatgt cagaattttt
    12241 gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag aggttctcga
    12301 atccggttgg atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt
    12361 gacgggaaat cagcatgcca tcgcggtcag ttcagccacc gccggaatgc atatcacgct
    12421 aatggcgttg gaaattggca agggcgatga agtgattacg ccttccctga cctgggtttc
    12481 aaccctcaat atgatttcct tgctgggtgc aacgccggta atggtggatg tcgaccgcga
    12541 tacgctgatg gtcacgcctg aagcgatcga atcagccatt acgccacgca ctaaagccat
    12601 cattccggtg cattatgccg gtgcgccagc agatattgac gccattcgcg ccattggcga
    12661 acgttacggc atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg
    12721 gcgacatatt ggcgcaaaag gtaccgctat tttttcattt catgccatta aaaatattac
    12781 ctgtgctgaa agtggcctga ttgtaactga taatgaaaac cttgcccgcc agctacggat
    12841 gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc
    12901 gcaggctgaa gtcttaacac cgggctataa gtacaatctg accgatatta acgccgcgat
    12961 tgccctgaca cagttagtca aattagagca cctcaacacc cgtcggcgcg aaattgccca
    13021 gcaatatcag caagcactgg cagctctccc ctttcagcca ttaagccttc ccgcctggcc
    13081 gcacgttcac gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag
    13141 tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt tacatttccg
    13201 caccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt taccgaatac
    13261 cgaatggaat agcgaacgca tctgttcgtt gccgctgttc ccggatatga ctaccgccga
    13321 tgccgaccgc gtcatcacag cccttcagca actcgcagga caataagcca tgtttgaaat
    13381 ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg aaagcttacc
    13441 ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt atgagatcct
    13501 gctgattgat gacggcagta gcgataattc cgcgcatatg ctggtcgaag cctcacaagc
    13561 ggagaacagc catattgtgt ctattttgat taaccgcaat tacgggcaac attcagcgat
    13621 tatggcggga ttcagtcacg ttactggcga cttaattatt acccttgatg ccgatctcca
    13681 gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt
    13741 agggactgta cgccagaacc gccaggacag ctggtttcgt aaaacagctt cgaagatgat
    13801 taaccggctt attcagcgca ccactggcaa agcgatgggt gactacggtt gtatgctgcg
    13861 cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgca gcacctttat
    13921 cccgattctg gcgaatatct tcgcccgccg tgcaattgaa attccagtac atcatgccga
    13981 gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga tgtacgacct
    14041 ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcggca gcattattgc
    14101 gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct tcggaccaca
    14161 atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta ttggcgctca
    14221 gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg atgtccgcgc
    14281 ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa atgaataatg
    14341 aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc tctgctggct
    14401 gccggttacg aaattagcgc catttttacc cataccgata atcccggtga aaaagccttt
    14461 tatggttcgg tggctcatct agcggcggaa agagacattc cggtttatgc gccggataac
    14521 gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt caccagaggt gattttctct
    14581 ttttattatc gccatcttat ttgcgacgag atttttcagc tcgctcccgc aggtgcattt
    14641 aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg
    14701 gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa tggtaaaacg tgccgatgct
    14761 ggggccattg tggcgcaact gcgcgttgcc attgcgccag acgatatcgc tattacgctg
    14821 catcataaat tgtgccatgc cgcgcgccag ctactggaac agacattacc cgccattaaa
    14881 cacggtaata ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga
    14941 acgccggatg acagtttcct tgaatggcat aaaccggcat ccgtactgca caacatggta
    15001 cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca gaaattcacc
    15061 gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac agccggggag cgtgatttct
    15121 gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg
    15181 ggcgacggca ttactatgca gggctcgcaa ttagcgcaga tgctgggcct ggtgcaaggt
    15241 tcacgcttga atagccagcc tgcctgcacc gcccgacgcc gtacccgggt actcatcctc
    15301 ggggtgaatg gctttattgg caaccatctg acagaacgcc tgctgcgcga agatcattat
    15361 gaagtttacg gtctggatat tggcagcgat gcgataagcc gttttctgaa tcatccacat
    15421 tttcactttg tcgaaggcga tatcagtatt cattccgaat ggattgagta tcacgtcaaa
    15481 aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc cgattgaata tacccgcaac
    15541 ccgctgcgcg tatttgaact cgattttgaa gagaatctgc gcattatccg ctactgcgtt
    15601 aagtaccgta agcgaatcat cttcccgtca acttcagaag tttatgggat gtgtagcgat
    15661 aaatacttcg atgaggacca ttctaattta atcgtcggcc cggtgaataa accacgctgg
    15721 atttattcgg tgtcaaaaca attacttgat cgggtgatct gggcctatgg cgaaaaagag
    15781 ggtttacagt tcaccctctt cctcccgttt aactggatgg gaccacgact ggataacctt
    15841 aatgcagcgc gaattggcag ctcccgcgct attacgcaac tcattctcaa tctggtagaa
    15901 ggttcaccaa ttaagctaat tgatggcgga aaacaaaaac gctgctttac tgatatccgg
    15961 gacggtatcg aggcgttgta ccgcattatc gaaaatgcgg gaaatcgctg cgacggcgaa
    16021 attatcaaca ttggcaatcc tgagaacgaa gcgagcattg aggaactggg cgagatgctg
    16081 ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc gggctttcgc
    16141 gttgtcgaaa gtagctgcta ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg
    16201 agcatccgca atgcccaccg ctgcctggac tgggagccga aaattgatat gcaggaaacc
    16261 atcgacgaaa cgctggattt cttcctgcgc accgttgatc ttacggataa accatcatga
    16321 ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc
    16381 gtctgctgga aatcttgagt aagcataata ttcaggccag catttttttc agcgtcggcc
    16441 cggacaatat gggccgccat ctctggcgac tggtgaagcc acagtttttg tggaagatgc
    16501 tgcgctcaaa cgcggcatcg ctttatggct gggatatttt actggcaggt acggcctggc
    16561 caggtaaaga gattggtcat gccaatgccg atatcattcg tgaagcggct aaacatcacg
    16621 aagtcggtct gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt aactgggatc
    16681 ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac tctggaagag attatcggtc
    16741 aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa
    16801 aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg ggccataccg ttccgtccac
    16861 tgcttgaatc aggaaaccct ggcactgcgc aaattccggt gaccttaccc acctgggatg
    16921 aagtgattgg tcgggatgtg aaagcagaag attttaacgg ttggttactc aaccgcatcc
    16981 tgcgagataa aggcacgccg gtttatacca ttcatgcaga agttgaaggc tgcgcttatc
    17041 agcataattt tgtggatctc ctcaaacgcg cagctcagga aggcgtgaca ttttgccctt
    17101 taagcgaact gttatcagag acgttgccgc tcggacaagt tgttcgcgga aatattgccg
    17161 gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg
    17221 ttaccttatc ggcatcttcg cgtttattgc ctgctattac ctgttaccga tcagcacgcg
    17281 tctgctctgg caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa tgctggcatc
    17341 cggcgactgg attgtgcccc atctgttagg gctacgttat ttcgaaaaac ccattgccgg
    17401 atactggatt aacagcattg ggcaatggct atttggcgcg aataactttg gtgtgcgggc
    17461 aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg acctggttta ctctgcgctt
    17521 atggcgcgat aaacgtctgg ctctactcgc cacagtaatt tatctctcat tgtttattgt
    17581 ctatgccatc ggcacttatg ccgtgctcga tccgtttatt gcattctggc tggtggcggg
    17641 aatgtgcagc ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt
    17701 tttactgctg ggaatcacct gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc
    17761 cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga aagatctctt
    17821 tatttacggc tggctggcgg ttatcagttg cgtactgacg gttctcccct ggggactggc
    17881 gatagcgcag cgggagcctg acttctggca ctattttttc tgggttgagc atattcaacg
    17941 ctttgcacta gatgatgccc aacatagagc tccgttctgg tactacgtgc cggtcatcat
    18001 tgccggtagc ctgccgtggc tgggattact ccccggtgca ctgtacacag gctggaaaaa
    18061 ccgcaagcat tccgcaaccg tctatttgtt gagctggacg ataatgccgc tgctgttttt
    18121 ctccgtcgct aaaggcaaat tgcccaccta tattctttct tgctttgcac ctctggcaat
    18181 gctgttggcg cattacgcgt tgctggcagc aaaaaataat cctctggcgc tgcggattaa
    18241 tggctggatt aacatcgctt ttggcgtcac tggcattatt gccacatttg tgatctcccc
    18301 ctggggacca atgaacacgc cggtgtggca aaccttcgag agctataaag tcttttgtgc
    18361 ctggtcgatt ttttcgctat gggcattttt cggctggtac accttaacaa acgtcgaaaa
    18421 gacctggtct ttcgccgcgc tttgcccgct ggggctggcg ttgctggtag gattttcaat
    18481 tcctgacaga gtcatggaag gaaaacatcc gcaatttttt gtcgagatga cacaagaatc
    18541 acttcagcca agccgctata ttcttactga tagcgtcggt gttgccgcag gtctggcatg
    18601 gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca ggtgagttga aatacggcct
    18661 taattatccg gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa actggcttaa
    18721 tcaacatcgt caggagggga ttattacact cgtgctttcg gttgaccgcg atgaagatat
    18781 caacagtctc gccattccgc ccgcagatgc catcgttcgt caggagcgtc tggtgctgat
    18841 tcagtatcgt cccaaatgat ctggctaaca ttagtctttg ccagcttgct tagcgttgcc
    18901 gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat
    18961 atcgtgctgt ggctgggact ggcgctggct tgtcttggtc ttgccatgat gctctggctg
    19021 ctggtctcgc agaacgtacc ggtaggcatt gcctacccga tgttaagtct gaattttgtc
    19081 tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt
    19141 ggggtggcgt tcattattgg tggcattgtg atcctcggga gtacggtgta atgggcctga
    19201 tatggggatt attcagcgtc attattgcct cagttgcgca attaagcctg ggttttgcgg
    19261 caagtcatct gccgccgatg acgcacttat gggattttat cgcggctctg ttagcttttg
    19321 gcctggatgc ccggatactg ttactcgggc tgctgggtta tctgctttcc gttttttgtt
    19381 ggtataaaac gctacataaa cttgcgctaa gcaaagccta tgccttgctc agtatgagtt
    19441 atgtgctggt atggattgct tcaatggtac ttcccggctg ggaagggact ttttcgctga
    19501 aagcactact gggagtagcc tgtattatga gcgggttgat gctgattttc ctgcccacga
    19561 caaaacaacg ttactgagtt ttccctgccg ctgtacactg ccgttcccac tcatcggggc
    19621 tataactgct tgccgagagg actttcaccg tatgcagctt ttcacgatta atacaataaa
    19681 gcgcattctg caaaggcgag agtaaatccc ccactttcac ggcgaagtca ctcttcactt
    19741 cggcgatcat ttttatcgcg ccgccagcat cgcagagcat aagtacatgt ctgttatctt
    19801 gtttattgca gcacgatttt ttgtgccagc cattccattg cattatcctg tttgctaaga
    19861 gttttcacat caatttacag attaacagat aaactcttta tcaacggcaa tggaatggca
    19921 gaaatgagaa gtgaaacgga gggttaatta ttgctgacgt tgcacccact cttttagcgc
    19981 ctgacgtgaa attttaatac cgccgttttt cagctccggc ggtaaagtca gccagcgcac
    20041 cggttgttga aaacgagcca gcttatcttt cacccattcg ccaagatcaa cggtttgctg
    20101 gtcatactcc accaccgcta ccggccgatg accaaactcc ttgtcggcaa cggaaacgat
    20161 aaacacctgc aaaaccgcag gatgtgcagc aataacacgc tcgacttctt ccggctgaat
    20221 accctcaccg ccactgaaga acagattgtc taaacgtccg acaatggtca gcttgccatt
    20281 atgcatctca ccgcgatcgc gcgtagcgta ccagccttcg tcattaacca gtgaaaccag
    20341 ttgcccgtta cgccagtaac cttctgccat actggcagcc cgcagccaca cttcattatt
    20401 aacgattttc acttcccgac ccggcagcgg caaaccaacg tctgccaggc cgtcggcttc
    20461 tttcgcacat accgtggagg caaactcggt cagaccatag ccgcaaaagc aacgaatccc
    20521 ctgctcgcgc gcctgttccg tcaactcgac cgggatagcc gcgccgccaa gtaacaccgc
    20581 tttcagggaa acggaactgc ggttaaccag caaacgccag agttgtgttg gcaccagtga
    20641 agcgtgagta cagcctgcca gcatttgctc caatggctgt ttatcacgca ccgtcatccg
    20701 cgcaccagcg tataaccagc gccacataat tccctgaccg gagacgtgaa acagcggtaa
    20761 agagagcaac cagtcatcgt gatcgccaaa cggaatcagc gataacacac cttgcgcact
    20821 ggcaagatgg gcctgataag tatggacagc ggctttcggc aaaccggtag aacccgaggt
    20881 caacgtcatt gagcacagac gcgtcggctg ccacgcagcg gcatgtacgc cttcaaccag
    20941 ctgaatgtgc agcgacgtta atgccggaaa cgtgttttcc ccatccggca ccagcgcaaa
    21001 ttgcagcgtc agattgggca gcaattcttc aagcaacggt tgcggcagct gagggttcac
    21061 gggcaacacc cgcgccccgc attgcagtaa cgccagccag gcgagcagcg tttgcggcgt
    21121 attccacgcc cgcaacatca cgccgctgcc ctcaaccacc ccctgcgccg caaagcccgt
    21181 agctaactca tcgacgcgag cacaaagctc gcgccagttg agttgctcgt cattaagacg
    21241 taaggcgatg gcttctcccc gcacttgccg ccagtgacgc cacggccagt cagagaagat
    21301 cataacaact gctcctgtac atcagcgtcc actaacggca gtgggctccc cggccagcga
    21361 cgaacctgct gcgcctgcat cagatccagc gtgtccagtc ccggaatggt gtctggcgtt
    21421 aaccaggcag caatccgcgc cagttgcgtt agacctaagc tcgactcaat ggaagaactg
    21481 atcaccgccg tcagccccag cgcatgcgcc gcctgtacct gctcgcgtac tttttccaga
    21541 ctgcccgtga gcgtgggttt gataactacc gcgcgcacgc cctcttcagc cacaaaggca
    21601 aaatccggct cgcgcaggct ttcatcccag gcaatggcaa tgccggtttc acgggcaaac
    21661 gctcgcgaat catcgcgggt tttgcacggc tcttcgagaa acgcgatgcg gtggcgataa
    21721 tccgggttaa catatttggc aaactgctga cctttcagcg gtgtccaggc gcgatttgcg
    21781 tcaagacgca aatgcagatc cggaattgcc tccagcaaca gattcaccac catgccgtcg
    21841 cgcaccgctt cgtacaatcc cactttgacc ttcgccactt tctcgcccgg catatcggca
    21901 agtttgagga tcagatcgtc cggatcgcca ttacacagcg gtgccgcacg gtagttggct
    21961 gcttgcggca acgtatctgt cagttctgcc aatgcacagc ttacgccaaa ggccacggaa
    22021 ggcatctgcg gtagctcgca atcgcctgcc agccagttat ttacccaggc aagcagcacg
    22081 ctttgcgcct cttcccagtt ttcctggctg aagcccggca gtggggagat ctccccccac
    22141 ccttcgcgct cgccctcacg caggcaaacg tacagcccgt cgcgggtttt taaccgcctg
    22201 tcgcgcagaa ccacccccgc gtccatgggg atctgccagc ggtatacctg cgcgctacgc
    22261 attacggatt ccgtttaaat ttgctgaaat caggctgacg tttctggttg aaagcgttgc
    22321 gaccttcctg accttcttcc gtcatgtaga acagcatggt ggcgttgccc gccagctcct
    22381 gcagccccgc ctgcccgtca cagtcggcgt tcagcgcagc tttcaggcag cgcagcgcca
    22441 tcgggctgtt ttgcagcatt tcgcggcacc aacggacggt ttctttttcc agatccgcca
    22501 gcggtaccac ggtgttgaca aggcccatat ccagcgcctg ttttgcgtcg tactgacggc
    22561 acaggaacca gatttctcgc gcttttttct gcccgacgat acgagccatg taggaagcgc
    22621 cccagccgcc gtcgaaggaa ccgactttcg ggccagtctg accgaagatg gcattatctg
    22681 ctgcgatagt caggtcgcac atcatgtgca gaacgtgacc accgccgatg gagtagccag
    22741 ccaccatcgc gacaaccggt ttcggacagg tacggatctg acgctggaag tccagcacgt
    22801 tcaggtgatg tacgccggaa tcatctttat agccgccgta atcaccacgc actttctggt
    22861 caccaccgga gcagaacgct ttatcgcctg cgccagtcag aatgatcacg ccgatattgt
    22921 cgtcataacg cgcatctgcc agtgcctgga tcatctcttt taccgtcaga ggacggaagg
    22981 cattgcgcac ctgcggacga ttaatggtga tttttgcgat accgtcggtg gatttttcat
    23041 aacgaatgtc ctcgaaacct tcggagcagt cgtgccattc aaccggtgcg taaggcattg
    23101 cttcatcagg ataaatcata ttgtgtcctt tagtcagaaa cgcaagatct gcgccagact
    23161 tgcgattacg ccagcgggat tttcccgatg cgcgttatgt ccggcgcgag gaatgacatg
    23221 gcagtcggca gccagttccg ccgccagggc gcggaatttg ctgtcacgtt caccacataa
    23281 ataataaaac gcaaatgtgc gggcgctaag gttagcacgt aaatcaggct ggacggcgag
    23341 agaagtcgcc tccagcatgg cggcaagggt tgcgccatta ttgttgctgc gcagcgccac
    23401 cagctcccgg cgttgatcgt cattgagcga ggcaaaaaca ggctgttgat accagtcggc
    23461 aaataccgcc gttaacggtt ctgtgcgaaa acgctgcgcc cattggcgat cggaacgctg
    23521 acgttccgca cgttgttcag cattttgcag ccccggatgc ccgccttcga caataacccc
    23581 acaaagcccc gccagcccct ggcaagccgc catcatcgcc acccgtccgc caagcgagta
    23641 ccccaccagc cagaagtcaa ggatgttgta actaaccaag gttttacgca gtaagtcggt
    23701 gacatcatca aatccatcga cgctaatcgc cgccgaacca ccatgaccta ggagatcaac
    23761 atacaaccgt gagtagtcgg caaacgcctc gcccacttct tgccattcgt ggcaatcgcc
    23821 ggaaaaaccg tgcaaaaaca ccagccaggg taaacctagt tttccgtgtt ttgcctgcgc
    23881 gtgcaggatc ataaatggct tacctgcgcc agaagttgct ggagcgtttg cgcaccatcg
    23941 gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg tgcgccaggc gtcggcaaat
    24001 gccgtttcaa gttcctgcca gttttgcgga tgatgatatt tcagctcgaa catcgcggcg
    24061 gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac gctcacgctc gctttgcggc
    24121 gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca ccacaattaa taccagcggc
    24181 gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt aaagcgcgga gagatcgccc
    24241 acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc ctgcggcggt cgaaagcagt
    24301 ccgtcgatac cgctggcccc tcggttgctg tacaccgggt aacctgccgg aagttgcgaa
    24361 agcgcatcaa tcagacgtac caccaggctg ttaccaacaa acaattgccc ctgttcaggc
    24421 agatagtcgc tgatgcgatg cgccagttgc gcttcgccaa acgcatcacg gcgggcaata
    24481 accgcctgca ttgcctgttc cgccaggcgc gggatttcaa cgcaccaggg ctggcgtttt
    24541 tctgccggat gcagctccag ccagtcggca atattggcaa ttaagcgacg tccgcgatgg
    24601 tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc agtactcttc tggttcacag
    24661 cttgcctgcc attgcaggag ccgtttcccc gtcaggctac ttcccagttg caccacaatt
    24721 tgcgcctgct gcagctcgct ggtcgctttg gcattgccta accaaagatc ggcacacggc
    24781 agcggctgcc cggtttgtga cagcacatcg ccaatcagcg gccagccaag agtttgcgcc
    24841 cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc cggcaaccac cacgccgcgc
    24901 ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt ccagacgagg cgcttcacgc
    24961 agccacggtt tgtcgtcctg ccaccagtca cccagacgct gttgccagct aagcccggta
    25021 tcgtccattt cgccatacag cggttcagca aacgggcagt tgatatggac tccccccgca
    25081 tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc aacgtgcggg gatatcctgg
    25141 gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagg cgaacattcc cggctggcga
    25201 attgcctgat tcgcgccgca gtcaattagc tccggcgggc gatcggcggt taagagaatc
    25261 agtttttctc cggttaaccc ggcttcaatc agtgccggat agagatttgc caccgccgtg
    25321 ccggaggtca caatcaccgc caccggctgc ttgctgactt tcgccagccc cagcgccaga
    25381 tgccccaacc cacgctcatc gaaatgggtg tggtgaatga atgcggaatt ctccgccgcc
    25441 gctaacgtta acggtgtaga acgcgagcct ggggcgatac agatgtgtct gacgccgtga
    25501 cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt taaatgcgct tactgacatg
    25561 agtttgtccg gtatcaatat tgcggctaag tataaggagc tacaaaaata gattattgat
    25621 atgaatcggt aatgatgcga ctcattacta ttccatttgt aataaagtac gcagccctgc
    25681 cgctttgttg tcgatttcct gccactcttg ctcggggtcg gaaccacgga caatgcccgc
    25741 gccagcatat aatcgcacga cattgccgct aatttttgct gagcgcaggg aaacgcagaa
    25801 ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg taccattctc gggtgaacgg
    25861 ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt aagccagcaa ctgctgccgt
    25921 tggctgcaac tgatgtaaac agatcacatc atccgctttg ttgagtgaag tccagataca
    25981 gcggcgaaga tgctgcactt tacgcagacg cagtacctgc ggcggtaaaa catccagcgt
    26041 ctgggtatcg gcctgtaatc gttggcagat atcttccacc accagcatgt tctcgcgctg
    26101 gtttttatca tccgccatca gccactctcc taactgctgc gcctgcttat catcaggatg
    26161 atttgctact gttcccgcca gcgcttcagt acgcagcgct ttgtcacgcc gccgccataa
    26221 ccgttccggt gaagagccaa gaaaagcatt ttcgccatca aaggccatgt aaaaatggta
    26281 gcaattcaga ttcagtcgac gactggcagc catcatcgcc gccgcgttga ccggacttgc
    26341 gaaatgcagg tcagttgccc gagcgagcac cactttgtcg agctcacctt cggcgatggt
    26401 tttcgttgcc agttcgatta attgcgtcca gcccgttttg tccggccagt gttgttctcg
    26461 cgtggtggtt aaatgtaacc caggcaaggg cttgatactc accagtgtgg cgataaattc
    26521 ttttgcctga atcgcatcgt gctgaaggga gctttcgctg aatagcgtca gccgcagcgt
    26581 ggctttaccg ccacagcgtc gccattccag gcggggtaaa agtaaattgc cctgcgacgg
    26641 gtcaaaagca ttcagccccc aaatgcgtaa gtcagcgtgt tccgggtgct ggcgaagaaa
    26701 acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg cccaggacgg cagcttcttc
    26761 atcaccatta cgttgttgcc agtagaattg cgggtacgtt tgctgactgg ccagccagct
    26821 caaggcatca aaagcgtctt tgagagggaa aggaatatcg ataacccgaa tgccgggtgt
    26881 cgccggaatc tcttgcgaca aatggcgcag tagattttcc agcgccgtag taagtgattg
    26941 cacgcggacc tctccctgtt aaaagcctca cattatacgg ggtactacaa aaaaaatgca
    27001 gtaccccggt gtagggaggt tttaacggcg tgccagcaac agtcctagta ccagcccgac
    27061 ggccgcgccc acaccaattc cttgccaggg tttttcgtgg acgtagtcat cagcacgata
    27121 aacagcctgc ttcgcccgat agtaataact gtctgaagcc tggctaaccc gttttttcac
    27181 atcatccagc gctttttctg catgcgcttt cagctcaaca tatttctgat cggcgggatc
    27241 gccagaggag cggagcacct cttccagtgt ttcactaagc agcgtcaggt cgtcatcgat
    27301 acgtgtatca ccaaactgat tagacattct cgttctccat ttgcgtaaaa cctgtcacta
    27361 actatagaca agagttgttg attacgcctg aattacctcg cgtgccatcc caatgtgtgg
    27421 gataccatct tcttcataga cctccgtcac agggataaaa ccgaaactct ggtagaagtt
    27481 ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca ggccagtgat gcgtacatgt
    27541 ttcgagtgtt ttactcatca gctgctgccc cactttttca ccacgcaacg cttcactgac
    27601 aatcacccga cctataacga ccggctcaag atcatcatca cttttcagaa tcctcgcata
    27661 cgccaccagt tcatcatttt tccaaccgag aatatggcga ttatcccccg tcaaatcatc
    27721 accatcgata tcctgataag ggcaattctg ctcgacaaca aacaccgcgc aacgcagttg
    27781 taataaggca taaagctggg aaaccgataa ttcagagtga tgcagatctt gccattcaat
    27841 cataatgctc tccttttaga tgtatgcctt atactagccg gaatattttt tgaaacgtga
    27901 tgaactcatg gaattaattt ttttaggtac ttcagccggt gtgccaaccc gcacgcgcaa
    27961 tgtcacggca atattgctta acctgcaaca tccgacccag agcggacttt ggttgtttga
    28021 ctgcggtgaa ggcacccagc atcagctact gcataccgcc tttaaccctg gaaaactgga
    28081 caagattttt atcagtcacc ttcatggcga tcatcttttt ggtttacccg gcttgctgtg
    28141 cagtcgttct atgtcaggca ttatccaacc cttaacgatt tatggtccgc aaggtatccg
    28201 tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg accgattatc cgctggaaat
    28261 tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg cgcaaagtaa ccgcttatcc
    28321 gctggaacac ccactggaat gttatggcta tcgtattgaa gaacatgata aaccgggtgc
    28381 attaaatgcc caggcattaa aagctgctgg cgtgccgcct ggcccactgt ttcaggaatt
    28441 aaaagcgggc aaaacaatca cgctggaaga tggaaggcag attaacggcg cagattactt
    28501 agctgctcca gtgccaggta aagcgctcgc tattttcggc gataccggcc cctgcgatgc
    28561 cgcacttgac ctggctaaag gtgtcgatgt catggtgcac gaagcgacgc tggatataac
    28621 catggaagcc aaagccaata gtcgcggcca tagctctaca cgccaggctg cgacactagc
    28681 ccgtgaggct ggagtcggca agctaatcat tacccacgtc agctcgcgct atgatgacaa
    28741 aggttgtcaa cacctgttac gtgaatgcag gtcaattttc ccggcgactg aactggcgaa
    28801 tgatttcacc gtgtttaacg tttaactctg cttattatta aaaacagggt gaaacttgcc
    28861 ctgttatagc aacccgcgct acatatatcc acccacacag gcatattgag cgatttttga
    28921 tatttattca tctccttacg cactatattc agagcaataa gttgcctctt ttcatcttga
    28981 ttcagcacaa cctttgcaac taaaaggagc ccatgatggt tacagttgtc agcaattatt
    29041 gtcaattatc tcaaacccaa ctcagtcaga catttgcaga aaaatttact gtgaccgagg
    29101 aattactgca gtctttaaaa aaaacagcgt tatccggaga tgaagaaagt attgagttac
    29161 tgcataatat tgcgttaggt tatgatgaat ttggaaaaaa agctgggtaa tgctgccaac
    29221 ttactgattt agtg
//
LOCUS       DBKELA010000046        29194 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.046, whole genome shotgun sequence.
ACCESSION   DBKELA010000046 DBKELA010000000
VERSION     DBKELA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.046"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="ACUQR0_002017"
     CDS             complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="ACUQR0_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HHV9109064.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTVEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1174..1875
                     /locus_tag="ACUQR0_002018"
     CDS             1174..1875
                     /locus_tag="ACUQR0_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HHV9109065.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1898..2062
                     /gene="yhaL"
                     /locus_tag="ACUQR0_002019"
     CDS             1898..2062
                     /gene="yhaL"
                     /locus_tag="ACUQR0_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HHV9109066.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2274..3584)
                     /gene="cyuA"
                     /locus_tag="ACUQR0_002020"
     CDS             complement(2274..3584)
                     /gene="cyuA"
                     /locus_tag="ACUQR0_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HHV9109067.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3612..4943)
                     /locus_tag="ACUQR0_002021"
     CDS             complement(3612..4943)
                     /locus_tag="ACUQR0_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9109068.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="ACUQR0_002022"
     CDS             complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="ACUQR0_002022"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HHV9109069.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6654..7043)
                     /locus_tag="ACUQR0_002023"
     CDS             complement(6654..7043)
                     /locus_tag="ACUQR0_002023"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HHV9109070.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="ACUQR0_002024"
     CDS             complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="ACUQR0_002024"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HHV9109071.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="ACUQR0_002025"
     CDS             complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="ACUQR0_002025"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HHV9109072.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="ACUQR0_002026"
     CDS             complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="ACUQR0_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HHV9109073.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="ACUQR0_002027"
     CDS             complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="ACUQR0_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HHV9109074.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="ACUQR0_002028"
     CDS             complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="ACUQR0_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HHV9109075.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14314..14532
                     /gene="tdcR"
                     /locus_tag="ACUQR0_002029"
     CDS             14314..14532
                     /gene="tdcR"
                     /locus_tag="ACUQR0_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HHV9109076.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            14788..15327
                     /gene="yhaB"
                     /locus_tag="ACUQR0_002030"
     CDS             14788..15327
                     /gene="yhaB"
                     /locus_tag="ACUQR0_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HHV9109077.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15349..16526
                     /locus_tag="ACUQR0_002031"
                     /pseudo
     CDS             15349..16526
                     /locus_tag="ACUQR0_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(17136..17512)
                     /gene="rnpB"
                     /locus_tag="ACUQR0_002032"
     ncRNA           complement(17136..17512)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACUQR0_002032"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17545..18690)
                     /gene="garK"
                     /locus_tag="ACUQR0_002033"
                     /pseudo
     CDS             complement(17545..18690)
                     /gene="garK"
                     /locus_tag="ACUQR0_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(18787..19677)
                     /gene="garR"
                     /locus_tag="ACUQR0_002034"
     CDS             complement(18787..19677)
                     /gene="garR"
                     /locus_tag="ACUQR0_002034"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HHV9109078.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(19707..20477)
                     /gene="garL"
                     /locus_tag="ACUQR0_002035"
                     /pseudo
     CDS             complement(19707..20477)
                     /gene="garL"
                     /locus_tag="ACUQR0_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(20493..21827)
                     /gene="garP"
                     /locus_tag="ACUQR0_002036"
     CDS             complement(20493..21827)
                     /gene="garP"
                     /locus_tag="ACUQR0_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HHV9109079.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            22202..23773
                     /gene="garD"
                     /locus_tag="ACUQR0_002037"
     CDS             22202..23773
                     /gene="garD"
                     /locus_tag="ACUQR0_002037"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HHV9109080.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(23825..24649)
                     /gene="agaR"
                     /locus_tag="ACUQR0_002038"
     CDS             complement(23825..24649)
                     /gene="agaR"
                     /locus_tag="ACUQR0_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HHV9109081.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            24898..26178
                     /gene="kbaZ"
                     /locus_tag="ACUQR0_002039"
     CDS             24898..26178
                     /gene="kbaZ"
                     /locus_tag="ACUQR0_002039"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HHV9109082.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            26201..26674
                     /gene="agaV"
                     /locus_tag="ACUQR0_002040"
     CDS             26201..26674
                     /gene="agaV"
                     /locus_tag="ACUQR0_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HHV9109083.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            26685..27464
                     /gene="agaW"
                     /locus_tag="ACUQR0_002041"
     CDS             26685..27464
                     /gene="agaW"
                     /locus_tag="ACUQR0_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HHV9109084.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            27454..28263
                     /gene="agaE"
                     /locus_tag="ACUQR0_002042"
     CDS             27454..28263
                     /gene="agaE"
                     /locus_tag="ACUQR0_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HHV9109085.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            28350..28784
                     /gene="agaF"
                     /locus_tag="ACUQR0_002043"
     CDS             28350..28784
                     /gene="agaF"
                     /locus_tag="ACUQR0_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HHV9109086.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            28781..>29194
                     /locus_tag="ACUQR0_002044"
     CDS             28781..>29194
                     /locus_tag="ACUQR0_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HHV9109087.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         7593 a         7623 c         6890 g         7088 t
ORIGIN      
        1 ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc agcctacaaa
       61 ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag cgcatcaggc
      121 agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata gattattttc
      181 cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc atactgtcac
      241 gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc
      301 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      361 ccagtaatgc ctgacgttta tcttcaaccg tgctcaccgt taagcgcggc tgtttgtcca
      421 gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca attccacgat
      481 atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      541 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      601 gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc cgttcccacg
      661 cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc gccagtttgt
      721 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg
      781 tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg gcttccagca
      841 aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg
      901 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
      961 cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg cgatcgatcg
     1021 catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat
     1081 tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt
     1141 accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag
     1201 tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac
     1261 ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc
     1321 ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg
     1381 gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag cgccggagag
     1441 gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct cagcaaagac
     1501 aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga gaatccgctg
     1561 attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc agagggtgcg
     1621 atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct cgacaaaggc
     1681 gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa
     1741 tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga tggggcgttt
     1801 attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata
     1861 gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat cgaccaaaaa
     1921 gcgccagcca gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca aaaattttgg
     1981 ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa cgcgtttagc
     2041 cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg ccttatccgg
     2101 cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata agatgcgcca
     2161 gcatcgtatc cggcatcagg tacaaaacac aacaacatca acaagttgag caccccgtag
     2221 gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg cgtttatctg
     2281 gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga atggcttgct
     2341 aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc ttcattgccg
     2401 gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga agcactggtc
     2461 gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg
     2521 atcatactgc tgatcgccat cgagatagtt tcataacgcc catccaccag ccatgccatc
     2581 ccggcggcgg cccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac
     2641 tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca
     2701 tcatccgctc cgaagtgttc tgctacaacc accacaggca ttattgcagt gatcccctgg
     2761 ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg cgcatcggat
     2821 gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa accgcgctcg
     2881 cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa accttcctgc
     2941 gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc aaatgggact
     3001 tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt cagcggcgac
     3061 tcttgctcgc cctctgccac acacgcctgg tgggtaaaca ccacaccatt gtgcgtctca
     3121 atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt ctcaccgttc
     3181 cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt aacagagact
     3241 ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc gtctttcagc
     3301 acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc aatcggcagc
     3361 cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag atttggcgaa
     3421 acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac cgccgccgcc
     3481 agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc ctgaacggcg
     3541 aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa aaccttaaaa
     3601 tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa accagtgacg
     3661 ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt gtaaaccagc
     3721 cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga ggtgaagctc
     3781 aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa aatcatgatg
     3841 ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg caggatgttc
     3901 attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa tgcagtcatg
     3961 acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc gtcaccgcta
     4021 ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt taccgcttcg
     4081 tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac aaacaaaatg
     4141 ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat tgatttttcc
     4201 cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacagaat cgacgtcagg
     4261 gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg tagcgaaccg
     4321 acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac taccagcagc
     4381 ttggtcagca ccatgtcggt cgaaattttg aataacaatt tctcgccgcg tgaagagatc
     4441 gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag caacccttcc
     4501 gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt ggaatagacg
     4561 aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc ccagttttta
     4621 cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc tgccagcgta
     4681 ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga tgagagcaaa
     4741 aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc cgcgccaatc
     4801 gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt gatcgcttct
     4861 cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc tgcaatgacg
     4921 cctttattcg atgcaatttc cataatttct cgctcaatat tttgtagggc ttattatttt
     4981 tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt tattagaata
     5041 aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat caagacttta aaataacgga
     5101 gatggataag aattttctac taaattaatc gcaagagaat attcattctc tatttgcggc
     5161 agatcacaaa aaaaaggtgc acatttgtgc acccaaggat gaaagctgac agcaatgtca
     5221 gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt tcgcggtatt tatcgttcat
     5281 atctttgccg gtttcataca tcgtctcgat cactttatcg agtgaaacac gcggtgccga
     5341 ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc acggcattaa tggcattacg
     5401 ttcaatgcac gggatttgta cctgtccggc aaccggatcg caggtcagcc caaggttatg
     5461 ctccatcgcg atttccgccg cattgcatac ctgcgccgga ctgccgccca gtagttcagt
     5521 taaccctgcc gctgccattg aacaggccac gccaatctcc ccctgacagc cgacctccgc
     5581 gccagagatg gaggcgttca ttttatacag cgcgccaata gcccccgctg ccagaaaata
     5641 gcgggcaatt gaccgctcgt ttaccggacg acggaactta tcgtaataag ccagcactgc
     5701 cggaataatg ccgcacgcac cgttagtcgg tgccgttacc acgcgcccgc cagctgcgtt
     5761 ttcttcacta accgccagcg cgtacatgtt gatccagtcg atgacattca tcggatcgtt
     5821 agagatgtta tcgctagaaa ccagctgacg acgcagcgct acggcacgac acggcacatt
     5881 gagcggacca ggcagcacgc cttcagtgtt catcccgcgt tcaataccgt catgcatcac
     5941 ttgccagata cgggcaaacc ctgcgtcaat ttccgctttg ctgcgcagcg ccagctcgtt
     6001 gtgcatcatc aggccagata tagacaggcc gttgtaatca cacattttca gcagttcacc
     6061 tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg tgcgacaggc cgaagtgttc
     6121 ttcttcgaca ataaacccgc cgccgacaga gtaataggtt ttacttaata gcgcttcctg
     6181 tcccttccag gcagtgatcc gcattccgtt ctcatggcga ggcaacattt cgggatggaa
     6241 gataatgttc tttgctacag gaaaatcaac aatatgcgca ccagatgcca ctggcagccg
     6301 cccgctgcgc gttactaact ctataaatgc agggatctca tcaatgacaa catcctgcgg
     6361 actgtttcct gccagtccca tgataatggc gacatccgtg gcatggcctt tgcccgtcag
     6421 tgacaacgac ccgtacagat cgaccacaat atggctcgtc gcggttaata agccgctact
     6481 ttccagccga tcaataaaac tttttccggc attcattggc cccacggtat gcgaactgga
     6541 gggaccaatc ccaattttga aaatatcgaa tgcactaatc atatccacac cctcggattg
     6601 ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc cgctcacgag gctttacgca
     6661 ctacgtactg cgatagcttc aatttccagc ttcacatctt tcggcaaacg cgcaacctgc
     6721 acacagctcc gggtcgggta ggtcgcctga tgctcatcga agaactgctt atagacttca
     6781 ttgatggtgg caaaatcatt cagatcggtg atgaacacgg tcatcttgat gatatcgccc
     6841 acgctcagcc cggcagcaac cacgatcgct ttgacgtttt cgaggcttaa acgtgcctga
     6901 tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa ccggaatttg cccggaggtg
     6961 aagaccatgc tgcctaaatc aacgccctga acataagggc cgattgcgcc tggggcacgt
     7021 tgcgtttcga taatcttttt catacatcct ccggcgtcag agcgcctggg taaaagtacg
     7081 tgaaataaca tcctgttgct gttcacgggt cagtgcgttg aagcgcacgg cgtagccaga
     7141 gacacggatt gtcaggttag gatatttttc cgggtgctcg atggcatcca gcagcatttc
     7201 ccgattcatt acgttgacgt tgaggtgttg accgccttcg acatccgctt cgtggtggaa
     7261 atacccatcc agcaggccaa caaggttggt tttacgtact ggatcttctt tgcccagcgc
     7321 cgcaggaacg attgagaagg tgtacgagat cccatctttg gcgtaggtga acggcagttt
     7381 cgccaccgac gtcaatgagg ccacggcacc tttgcggtca cgaccatgca tcgggttagc
     7441 gcccggcgcg aacggtgttc cggcgcgacg accgtccggc gtattaccgg ttttctggcc
     7501 gtacaccacg ttagaagtga tagtcagaat cgactgggta gggacggcgt tgcgataggt
     7561 tggcagcgct ttaattttct tcataaagcg ttcaaccagg tcgcaggcaa tgctgtctac
     7621 gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg atttcaaagt ccaccgccag
     7681 gccgttttcg tcacggattg gtttcacgcg ggcatatttg atggcagaca gggagtccgt
     7741 cgccaccgac aggcccgcga taccgcatgc catagtgcga tagacatcac gatcgtgcag
     7801 cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg tagtgaatga tattcagcgc
     7861 gctgatgtac tgcaccgcca gccagtccat gaagtgatcg aggctgtcca tcactttgtc
     7921 gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc ccgacctgaa tcttcagctt
     7981 ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc gccaggttag cgcgtgcacc
     8041 aaagaactgc atttgcttac caatcaccat tgggctgacg cagcaggcaa tcgcgtaatc
     8101 gtcgctgttg aagtcagtac gcatcagatc gtcattttca tactgcaagg aagaggtgac
     8161 gatcgacacc tgcgcggcat attttttgaa ggcaatcggt aattcttccg accaaagaat
     8221 ggtcaggtta ggttccggtg ccggccccat agtgtgcagg gtgtgcaaat agcggaagga
     8281 gtttttggtc accagcgtac gaccgtccag ccccatcccg ccgatcactt ccgtcgccca
     8341 gattgggtcg ccggagaaca gcgaatcaaa ttccggtgta cgcaggaagc gtaccatacg
     8401 gatcttcatg atgaagtgat cgatcagttc ctgtgcctgc tgctcattga ggacgccagc
     8461 tttaaagtcg cgctcaatgt agatatccag gaacgatgcc gtgcgcccca gcgacatcgc
     8521 gccgccattt tgcgatttca ctgccgccag ataagcgaag tagagccact gcaccgcttc
     8581 ctgtgcattc tgcgccgggc gagagatatc aaagccatat ttcgccgcca tttcctgaat
     8641 ctgcaacagc gcatgacgat gctctgccag ctcctcacgc agacggatgg tggcttccag
     8701 atcctcgcct ttttccagac gagactggag atcggcaaat tgcagttcgc gttcacgcac
     8761 cagataactg atgccataca gcgctacgcg gcgatagtca ccgataatgc gcccacgacc
     8821 atagccatct ggtaaaccgg tcagcacgcc agatttacgg cagcgcagca tatccggtga
     8881 gtaaacatca aatacgccct ggttatgggt tttacgcaga tcggtaaaca gatattcaaa
     8941 ttcactgtcc atttcacggc cataggcgtg gaatgaactt ttaatcatat taatgccacc
     9001 gaacgggtgt agcgcacgtt tcaacggcgc atccgtttgc aggccaacaa ttttttccag
     9061 cggctggtta atatatcccg catcatgagc ggtaattgtg gtggcaatat tggtatcgaa
     9121 atcaaccggc gcgtgggttg cgttttcgat acggatgcct tccattacct tttcccacaa
     9181 ttccgtggtg gcaggcgtcg cttcggcgag aaaagattca tcgccttcat acggtgtata
     9241 gttatgttga ataaaatcgc ggacattaat ttcgcttttc cagtccgtac ctttaaagcc
     9301 aagccatgcg tcagcgtaca gcttatcgct ggtatcaata tctaccttca tgaaaaataa
     9361 tctctctaca atacttcaac taaattatgc aaattctgcg ggcgcgttaa ctttacctaa
     9421 atgaatggca tccaaagcaa tcattttttc ttcgttagtc ggaataacga cgcaaatgac
     9481 atgcgcattt tcactggaaa caattcgctc gccaaaagag ttagatcgat tattcatttc
     9541 tgtatcaatc tctacgccta atacagccaa atgttccatg accagacgac gaattaaact
     9601 tgaattctct cctattccgc cggtgaatat aattccatcc agccgacgta atgaagctgc
     9661 atgtccggca atatgacggg caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg
     9721 ttcgtgacct tcatgccagg ctttttccag aacacgtaaa tccgaagaaa gaccggaaat
     9781 acctaataat cccgactctt tattcactac gcgttccagg tcacccaggc tctggttggt
     9841 ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg ccactgcggg tacccatcat
     9901 caagccttcc agcggcgtca ttcccattga ggtgtcaaca ctctgaccgt tgcgaactgc
     9961 gcagattgac gcgccattgc caagatgcgc cacaaccagg ccggagtcat cttccgccag
    10021 attcagcagc gaatgtgcgc gctgggaaac atagcggtgc gacgtgccgt ggaaaccata
    10081 acggcgtaca cctaactctt cataatattt ccacggcagg ccgtataaat aagcttccgg
    10141 agccatcgtc tggtggaaac tggtatcaaa taccgccacc tgagttacgc ccggaaataa
    10201 ttgctgcgcc gattcaatac cacttaaatt tgcgtaatta tgcaggggtg ccagtggaga
    10261 aacgcgacgg atattatcaa tgacttcatc ggtaataatg gcggactcgg taaaaatact
    10321 gccgccgtga gcgatgcggt ggccaattaa ggccacgctg tcatttaaat tccgtttttc
    10381 cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg tggtgagcca gcggtgctgg
    10441 ctctccccca tttacggata agaatgcatt ttccgagtta ataccgtcgg caatacctga
    10501 cattaatact tcacaatcgc tggcatcgag tacggaaaac ttaatcgaag acgaaccaca
    10561 gttaataacc aaaacaaccg gaaattcatt catctctttt ctcatcctga gttacggatt
    10621 aaaacagttt gtatacgatg ttcaggatgg tcagcagacc aatcacggta acaaacacgt
    10681 tatccagacg accacggtat ttcgccagag acggcgcttt acggatagca tacatcggca
    10741 acaggcacag cagggatgcg ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt
    10801 tcgggttggc gtaggcaaca acccaggtgg agcccatgat gaagatcatg ctgagagtat
    10861 tcagtttacc cagcgacact ttggttttgt cacctttata accgaacttc agaatcagac
    10921 cattcaagcc ttccagcgtc cccagatagt gaccgaagaa agatttgaag atagccacga
    10981 gtgcgatgat ggaagccgca tattccagtg taatcgcgaa cgttgttttc gtaccggtca
    11041 tggacgcaaa gtggttagcc agataagaaa gcactggaat attctgcgct ttggcttccg
    11101 ccatgttggc cggagacaga gtaaacaggc agctaaaggc aaagaacatc accactgcaa
    11161 ccatcagcat gctggcacga gaaatgattt gggaacattt acgttcggtg aagtcgcgac
    11221 cgaagtcttt ctcatactct tcacgtttag aaaccacgaa ggaagagacg attggcgaga
    11281 agttaaagga gaaaaccatg atggaaatcc ccagccagac agtgatcagg ataccgtcat
    11341 gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat aactgcagag ttccagtaag
    11401 ggatcagcga cagagaaatc agcaccaggc tggcgataaa cggccatacc aggtagctca
    11461 tcactttaac catcagatcc ttaccaaacc agatgacgaa agccatcagc agcaacagga
    11521 acagcgccac aaagccgcga ttcagcggtg caaatccgag ctggttttcc cagaacgtca
    11581 taaaggtatt ggtaatagta acgccataaa tccacagcag tgggcaaatc gcgaagaagt
    11641 acaggaacgt gataaccacg ccgccagttt taccaaaatg ctcttccacc gtttccgtaa
    11701 tgttgccgga agggttagag ccagaaagac acagacgcgc cagcgcccgg tggcaataaa
    11761 atgcgatggg gtatgccaat accaacatca gaagaatcgg gatcagtccg ccaaaacctg
    11821 cgcggatagg gaagaacagc accccggcgc cgattgccgt accaaacaag cctaacgtcc
    11881 atgtggtatc tgatttacgc caggacgatt gttttgtctg gctggataca atgctatctg
    11941 aagtactcat atcctatcct caacgaatta attaagcgtc aacgaaaccg gtgatttgag
    12001 agacgcgaga aagatcgata ttgccgccgg aaataatact gacggttttt ctgttttgaa
    12061 tatattggtc taatttaccg cttaataatg cagcacatgc cagagcgcct gcgccttcgg
    12121 tgacgacttt attgcgctga attaaggcaa tcatactgtt tctgatttcg tcttcgctga
    12181 ccagcacgat gtcatcgact aattcacgaa cgatttcgta agttaaatta cccgggcggg
    12241 agacatcaca accatccgcc agggtgccgg tagttcggtg cgtggttatt tctccggagt
    12301 ggaaagaagc cgccatgccg tgaacgtttt ctgactgtac gccaataaca cgaatggtcg
    12361 ggttaataga tttaattgcc accgcaatac cagcaattaa accaccacca ccaattggca
    12421 caatcacgtt atcgacatca tagagatctt ccataatttc cagaccaatc gttccctggc
    12481 cagcaatcac tttcggatca tcgtaaggtg ggataaaaat acgaccttcc atttcgacaa
    12541 tttcgctcac tttagcgata gtgtcgttga agttatcacc atgcagaacg acttctgcgg
    12601 agtagtcgca cgttgccgct actttggatt ttggcgcacc ttttggcatc accactttac
    12661 cgtcgatacc cagcatcgcg caggagagcg aaaccccttg cgcatggttg cccgcagaac
    12721 aggccaccac gcctttgcgt ttttccgcat cggtcagtga acttaattta ttaaatgcgc
    12781 cacgaatttt aaatgaaccc gtacgctgca tattttcaaa cttcaggaat atttcacctt
    12841 tgcaacgttc actaaaatag ttggagcgag gcatgcctgt tttataaatt cgcccagcca
    12901 gtcgttgttt cgcttcaata atatcatcaa tagcaaccgg cagatcgtat gtaatatgca
    12961 ttataaaacc tcttcgcctg aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac
    13021 agacaggtgg attatttatg tttaaataaa aacaaacaac taaccaactt ctattaattg
    13081 ccttcgtcta cacccattat aagatgaata ctctttggct aattccacca aaaccgatgc
    13141 tgctttttta atacgataat ttttcgacca taccgcggca tattgtgcca caggtaatgt
    13201 ttcttcaacc ggaatagtaa taaattgatt agaaccaaaa ggtgacgtca tatcacaagg
    13261 aattacagtt aagaaatcag cattgagaac aagattataa attgtcacga ctgagtcggt
    13321 tttaacgatg ttttcaatac tgatgccatt tctttgtaac gtagtaagca gttcgctgta
    13381 gtaccccata ttagtttgtg gcaacaccca ctgttcgttc ttcaacgact ccagcgtggt
    13441 ggtgccggtg catgttcggg acttactggc taccagcaca aactcggact cgaacagcgg
    13501 ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac gtaccaatcg caaaatccag
    13561 tcgaccgtcg cggattgccg gtaagaacga agacagttgc gcttcataca tagaaacctg
    13621 cgctttcggg aacacctctt tgaacttgtt gatcatccct gacataaaag taaaaccaat
    13681 caatgaagga aaaccaaatg agacttccac caccgcttca gaagacatac cgcttatctc
    13741 attaaccata tttttcattt cacgggtaat ggattcggaa cgggagagta acaattgacc
    13801 ggcaggtgtt aatgttacac cggtattttt ccgcaccact aattccacac caaaataatc
    13861 ttcaatatcg ttaatgattt tactgacggc cggttgagtt aaccctaatt cttttgcagc
    13921 cgagccgata gagccacttc taatgacttc ctgaaagact accaggtgct gcgttttcgg
    13981 aagaagaata gtgctcataa cgacctacgt taattacctc attgacggca tgaagtgtat
    14041 caaaatgaaa taaacaggat atgtgcgacc actcacaaat taactttcat tactttccaa
    14101 ggtatcgtta ttacaattaa atatctttaa atatcaacaa gttaaagcat aaaaatcagc
    14161 ataaaccctg atttttatca aaaaaaaatg acggggataa ccatatttta tggcgataac
    14221 atcattcgtt atgtggttaa tacaaaaaag gggctgagac catatttcaa gcaattaccg
    14281 gataattacc aggcaaaatt tgagttaaat ttaatgagca agttctcaaa ttttattata
    14341 aataaaccat tttcagcgat aaatactgcg gcacgtcaca ttttttcacg ctatttgttg
    14401 gagaacaaac atttatttta tcaatatttt aaaatttcga atacaggtat tgatcatctc
    14461 gaacaattga ttaacgtcaa ctttttctct tctgacagga cgtcattttg tgaatgcaat
    14521 cgttttccat aaattcttct cccctcatag gcgacgaata gcattttgtg ttgaggatca
    14581 caaaacgaat aattgctggt cgccgcgata aggtcagacg aagtcaacaa gggggatttt
    14641 cactgtgctt tacatcagcg taggacacag aatgttgctt cgtttactgt aacgccgggt
    14701 aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg aaaataagca tctattattg
    14761 tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc caattgcaca tatttttcac
    14821 ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca atagaaatat tgattattgg
    14881 acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg acgttaataa aatttacagt
    14941 ataagtaatg aactccggag agtattatct gcaattactg cattgaattt ctatcaaggc
    15001 gatgttcctt ctgtcatgat ccgaatccaa ccggaaaata tgagtccatt catcatagat
    15061 atttctacag gagaacatga tgattatatc atacaaacat tagatgtagg cacttttgca
    15121 ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa aagagctgga atgtattaag
    15181 gagacgattt ctaagtattg cgcaaagttc acccaaaaag aagccatctt aacaccccct
    15241 aggcacttta ataaaacctg cattacttca gattgttggc aaattctctt tttttcaccc
    15301 gatcatttta ataatgattt ttattgatta tttataaagg ataaaataat gttccctgtt
    15361 tcatcaattg gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa tgaccttcct
    15421 gaaagcccca taggaaataa cctcgaagca ctagcaccag gtatagaaaa actaaaacag
    15481 acctctattc aaatggtcac tttacttaac gcgttacaac caagtggaaa atgcattatc
    15541 actggtgatt ttcaaaaaga attagcgtac ttacaaaatg taattcttta taatgactcg
    15601 tctcttcgtc tggatttttt aggttataac acccaaatta ttcaacgatc ggacaatacc
    15661 tgcgaactta ccattaatga accgttaaaa aactaggaaa tatctacagg caatatcaat
    15721 gttaattgcc cattaaaaga tatttacaat gaaatcagga ggttgaacgt aatctttagt
    15781 tgtgggactg gagatatagt tgatctatcc tctctggact tacgtaatgt cgatttagat
    15841 tattatgatt tcacagataa acatatggct aatactattc taaatcattt taaattggat
    15901 tcaacaaatt ttactaatgc caacatgttt caggttaatt ttgttagttc aacacaaaac
    15961 gccacaatct cctgggatta tttactaaaa ataacgcctg ttttaataag cattagcgat
    16021 atgtattctg aagaaaaaat caagtttgtc gaaagttgtt taaatgagct tggagacatt
    16081 accgaagaac aattaaaaat tatgagattt gcaattataa aatctatacc aagggcaact
    16141 cttacagata aattagaaaa tgaattaaca aaagaaatat ataaaagctc atcgaaaatc
    16201 aacaattgct tgaacagaat taaattacca gagatgaaag aattctcatc aaaaaaaata
    16261 tatgattaca tcgatacaat cattgaagat tatgaaaata tcaaagaaaa tgcttatctg
    16321 gtcgtccccc aaattaatta tactatggat ttaaacatag aagactctag ctcagaagag
    16381 ttaataccct cgagaaagac gaaaattctc cggacaatgg ctttgaggtc ggggaatata
    16441 acacatatga agcatataac tcagagaatc aatattttac cagagaggac tatacgtata
    16501 attacgacct tttaaatgca atatagactt aataacttct tacaaagtct attattaata
    16561 ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa cttatcgacg cgggtttgaa
    16621 actgatggca accgcaaaaa tgcctgatgc gctacgctta tcaggcctac gccatctctg
    16681 caatatattg aatttgcgtg cttttgtagg caggataagg cgttcacgca gcatccggca
    16741 tgaataaagc gcactcaaca atctgaaacc cgccggaacg gtttattacg tacatcaggt
    16801 gaaactgacc gataagccgc tttcttttgg gtatagtgtc gtggacagtc attcatcttt
    16861 ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt acgtatatca ggtgaaactg
    16921 accgataagc cgctttcttt tgggtatagc gtcgtggaca gtcattcatc tttctgcccc
    16981 tccaaaagca aaaacccgcc gaagcgggtt tttacgtaca tcaggtgaaa ctgaccgata
    17041 agccgctttc ttttgggtat agcgtcgtgg acagtcattc atctttctgc ccctccaaaa
    17101 gcaaaaaccc gccgaagcgg gtttttacgt aaaacaggtg aaactgaccg ataagccggg
    17161 ttctgtcgtg gacagtcatt catctaggcc agcaatcgct cactggctca agcagcctac
    17221 ccgggttcag tacgggccgt accttatgaa cccctatttg gccttgctcc gggtggagtt
    17281 taccgtgcca cggactgtta ccagccgcgc ggtgcgctct taccgcaccc tttcaccctt
    17341 acctgatccc gcttgcacgg gccatcggcg gtttgctctc tgttgcactg gtcgtgggtt
    17401 tcccccccag gcgttacctg gcaccctgcc ctatggagcc cggactttcc tcccctccgc
    17461 ccgtctcccc cgaagaggac gacgacgaag cggcgactgt ctggtcagct tcggcgcgca
    17521 gtatagaggg tttgcgcgcc cttgtcaccc cgtgttgcgc attccaatcg ccagtgtcgc
    17581 ggcaatatta cgtgaagcac ggcagatatt gtcataagcc ccgcggaatg cttcgtccaa
    17641 cgtacctatg ctggtcaata cgctgaagac cgcatcaatg ccatgctgat gtacaacgcc
    17701 aacatcatcg gtcaggctac ccgcaatgcc aatcaccggt ttatggtact tcttcgccac
    17761 gtttgcgacg ccaatcggta ccttgccgtg aatactctgg ctgtcaatac gcccttcacc
    17821 ggtgatcacc agcgtacaat cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat
    17881 ttcaatacca cttttcagtt ccgcaccaag aaacgccatc agcaccgcac ccataccgcc
    17941 cgcagctcct gcgccgggga catcttttac atcaacatgc agcgcttttt taatgacatc
    18001 ggcatagtga gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg
    18061 tgggccaaag atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc
    18121 gacgcgaatg acgcaatctt ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag
    18181 agtattaaga ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc
    18241 ccccagcgcc tgtaccatgc ctgcgccgcc atcatttgta gcgctaccgc caatgccgat
    18301 aataatgttt gtcgcgccgc tctccagcgc ctacaggatt aactcgcctg tgccgcgtga
    18361 agtggtcacg agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc
    18421 catttcaata aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc
    18481 gcccagcggc cctgtaaccc aggcgtgacg ttcagaaccc tgggtggctg caatcatcgc
    18541 ttccaccgtt ccttcgccac cgtcggcaac cggaatagaa acgtactgtg catcaggaaa
    18601 aatttcccga aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt
    18661 ataagagtct ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag
    18721 ggcgtttctc ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg
    18781 acgtcattaa cgagtaactt cgactttcgc cagtttttcg tagtagcacg ccagggcgct
    18841 atgatccgcc gttcctaaac catctgctcg cagtgcctgc atcatctcca taaccgcagc
    18901 tgtgagcggc agttgcgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt
    18961 aatatgcaga tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt
    19021 ggcatccagc acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg
    19081 gttaacgccc gctttagttg ccagcgttaa cgcttctgac atcgcggcaa tattcagcgc
    19141 cacaatgacc tgattcgcca gtttggtgac gttacctgca ccaattgccc cggtatgcac
    19201 cacggaaccc gccattgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc
    19261 gcccaccatc actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc
    19321 atccagcata tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg
    19381 tgcgatagaa ctcatatcga tcaataccgt acctggcttc gcgccttcaa taatgccatt
    19441 ctcacccagc gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc
    19501 gcactgttca gcgatcgctt tagccgtaga ggctgtttct gcacctgcag caatcacgtc
    19561 agcaatagct tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt
    19621 tttactcatt ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc
    19681 aatcatctct cttgttgcgg tggtggttat tttttaaagg tatcagccag tttatgagtg
    19741 gcagagcgga agacgccgag atcgctgccg acagccacaa acgtcgcgcc ccattccaga
    19801 taacgacgcg catcggcttc gaccggcgcg aggataccgc tgggtttgcc gtgcgcgctg
    19861 gcacggttaa aaatgtgctg aattgctttt tgtacatccg ggtgtgatgc attgccgaga
    19921 tggcctaatg ccgcggccag atcgctgggg ccgacgaaga tgccgtctac gccttcggta
    19981 gcggcaatgg catcgacgtt atctacgccc tgctgacttt ctatctggac cagaatagtg
    20041 atgttcttgt tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga
    20101 gaaacggaga cgccgcgaat gccttccggt gggtaacggg ttgatgccac cgcctgctct
    20161 gcttcctctt ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga
    20221 cgcttaataa tttccggctc gttggtcggc actcacacta ctggcgcgct ggcgctgcct
    20281 ttcaaggcca ttaactgcgg aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc
    20341 agcaccagcc agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt
    20401 gctgaccagc aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg
    20461 aaaacatcgt tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg
    20521 tcgccaacta cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc
    20581 agtgctgcat tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca
    20641 atggaggcaa cattgccaaa gacgttaaag acgccgccac agaggccaac aatctctttc
    20701 ggcgcggtgt cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc
    20761 agcgccatca gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg
    20821 gaagccagca acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg
    20881 cgtttgatca gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt
    20941 gccggaatcg aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga
    21001 taaatcggga accaggtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa
    21061 aatacgccca acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgagaccg
    21121 cttgctgccg cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag
    21181 ataaacttca gctcttcctc agacatacgt gggtgatctg tcgggttatg aatcaacttg
    21241 atccacagcg ccgtcagcac aaaacaaatc acccccataa cggtaaagac gtgctcccat
    21301 ccccaggcga aagtcagcca gccaagcaga ggcgaaaaga gcgccagcga gaaatattgt
    21361 gccgagttaa agatggcgga ggcagtacca cgttctttcg tcgggaacca ggcggcgaca
    21421 atacgggcgt tcgccgggaa tgatggagct tccgagaatc ccagcataaa gcgcataaag
    21481 aacatggaga tccctgccca ggccagcggg aacatatcaa caaagccttg caggaaggtg
    21541 aacagcgacc agaaaaagag gctgtaggtg taaacttttt tcgagccaaa cttatcaagc
    21601 aaccagccgc cggggatttg catcagcaag taggcccagc caaaagcgga gaagatgtaa
    21661 cccatcgaaa ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc
    21721 gttgcacgat cggcgtagtt aacggcggta acaataaaaa taatcagtaa tattaaatag
    21781 cgggtatgca cacctttcct tttttcgtca acggtgtcca gaatcatttt atttacctcg
    21841 ggtacttatg ctgattttta ttattatggg gaaggtgtta tttataagtt tcatttatgc
    21901 cgtaacgaca atgaactcgg gaattagtat aagcagcgcg agaataataa tcattgtgca
    21961 aatgctaatt taattaatac tttttaaata ttattttgag catatgcaca taaggttgcg
    22021 cgctaaagca cagatttgcg ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt
    22081 gacattgatc acatttctgc gtttaaactc ctgacattct tatttcaccc aatgaaatca
    22141 tttattttta aatgagacca ggtcctcatt ttaataaccc ctggctggag aatattgcac
    22201 aatggccaac atcgaaatca gacaagaaac gccaactgcg ttttatataa aagttcacga
    22261 cacagataat gtggcaatta ttgttaatga taatggcctg aaagcaggaa cgcgttttcc
    22321 ggatgggctg gaattaattg aacatattcc ccaggggcat aaagtcgcat tgctggacat
    22381 tccggctaat ggtgaaatta ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat
    22441 cccacgcgga agctggatcg acgaatcaat ggttgtacta ccggaagcgc cgccgttaca
    22501 cacgctgcca ctggcaacca aagtcccgga acccttaccg ccgctggaag gatacacctt
    22561 tgagggctat cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg gcatcaccac
    22621 cagcgtccac tgtgtggcag gcgtggtgga ctatgtagta aaaatcattg aacgcgatct
    22681 gctaccgaaa tacccgaacg tcgatggcgt ggtggggctg aatcatttgt acggttgtgg
    22741 cgtggcgatt aacgcaccgg cagcagttgt acctattcgt accattcaca atatttcgct
    22801 gaaccctaac tttggcggcg aagtaatggt gattggcctg ggttgtgaaa agttgcagcc
    22861 tgagcgcctg ctgactggaa cggatgatgt gcaagctatt ccagtagaaa tcgccagcat
    22921 tgtcagtttg caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg atattttgca
    22981 ggtcgccgaa cgccatctac aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc
    23041 agaactggtt gtcggcatgc attgcggtgg cagcgatgcg ttttctggcg taacggcaaa
    23101 cccggcagtt ggctatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc
    23161 agaagtcacg gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgaaga
    23221 ggtcggcaaa cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa
    23281 aaccgaccgc agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaacgtggt
    23341 agagaaagcg ctcggctcca ttgctaaatc gggcaaaagt gcaattgttg aagtgctgtc
    23401 gcccggtcaa cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt
    23461 tgtctgtggc acgcaacagg tggcttcggg tatcaccgtg caagtgttta cgaccggtcg
    23521 tggtacgccg tacggcctga tggcggtacc cgtcattaaa atggcaaccc gcaccgagct
    23581 ggcgaaccgc tggtttgatt taatggatat taatgcgggc accatcgcta ccggcgaaga
    23641 aactattgaa gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa
    23701 gaagaaaacc ttctcggatc aatggggact gcataaccag ctggcggtgt ttaacccagc
    23761 accggtgacc tgataattca attcgtatta tgcccgatag cacacgttta tcgggcatat
    23821 tgagtcacag cgaagacgcg tgatccccga ccagaatcac ttcaacccca gcctttcgca
    23881 gtccttccag actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag
    23941 tatcaatgat cttatgtaaa ctggaacgat tgaatttact ggaatcggtg accacgatga
    24001 tccgttccgc aacttcgcac atccgacggt ttaaacgggc ttcatcttca ttatgtgtgc
    24061 tgacgccgcg ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat
    24121 tttgcagcga ttgctcagcc tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc
    24181 cgccggtcat cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac
    24241 cgttggtcat cgcaattacg tcagtgtact tgcgcatcag acgagcaatc tcaaaagtgg
    24301 tggtcccgga atcgaggatc acccgatgac ctggctgaat caactcaacg gcagctttcg
    24361 caacgctgcg tttcatcgcg gtgttcagtg cgcttttatc ttccactgat ggctcgactg
    24421 acggcgtcgt gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct
    24481 tttccagaaa cgccagatca ttgcggatcg tcacagtaga tacgccatac aatgccgaca
    24541 gatcgttaac ctgcacactc ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct
    24601 cgctggtgcc tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt
    24661 tcgtaaaact ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca
    24721 agcccacctt gcccattggc gcaagctact ctcgtttcag tgactttcat tacgtttctt
    24781 ttgtgaatca gatcagaaaa ctattatctt tcgttttatt tttatctcac catgacgcag
    24841 tatcaattga aacaaaacga aagatcaata tcgcagtaat ctgaactgga gaggaaagtg
    24901 aaacatctga cagaaatggt gagacagcac aaagcgggca aaacaaatgc aatttatgcc
    24961 gtttgttccg cacatccgct ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa
    25021 acgccgttac tgattgaagc aacctccaat caggtagacc agttcggcgg ttataccgga
    25081 atgacgcccg ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg
    25141 caggatgcgc tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgcca
    25201 gccgctcagg caatggccaa tgccgatgat ttgattaaaa gctacgttgc ggcaggattc
    25261 aaaaaaatcc accttgattg cagcatgtcc tgtcaggacg atccaattcc cttaactgat
    25321 gacatcgtgg ctgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgcgaa
    25381 cactttggcg aagccgatct ggagtatgtc attggtaccg aagtgccggt acctggcggc
    25441 gcgcatgaaa ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg
    25501 gaagcccatc gtcacgcctt tgaaaagcaa ggtttgaatg ccatctggcc acgcattatt
    25561 gccctggtgg ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc
    25621 gccaaagcga gcgccttaag ccagatggtc gaaaactacg aaacgctgat tttcgaagcg
    25681 cactctaccg attatcaaac gccgcaatct ctgcgccagc tggtgattga ccactttgcc
    25741 attctgaaag ttggcccggc gctgaccttc gccctgcgtg aagccctttt ttctctggcg
    25801 gcgattgaag aagaactggt accagcgaaa gcctgttccg gtctgcgtca ggtgctggaa
    25861 aacgtgatgc tcgaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg
    25921 cgtcgtctgg cgcgtggtta cagctactca gatcgcgtgc gctattactg gccggacagc
    25981 cagattgatg acgctttcgc tcatctggtt cgtaatctgg cggattcacc aattccgcta
    26041 ccgctcatca gccagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcaa
    26101 ccgacgccac gggaactcat tataaaccat attcaggaca tcctggcgca gtaccacaca
    26161 gcctgtgaag gccaataagc aaaacaaaga ggaacatgct atgccaaata ttgttttaag
    26221 ccggattgat gaacgcttga ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg
    26281 ggcaaatctg gtgctggtag ccaacgatga ggttgccgaa gatccggtac aacaaaacct
    26341 gatggaaatg gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagttat
    26401 cgacaacatt catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc
    26461 cgatttcctg acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat
    26521 gcactacgcc aatggcaaac aacaaatcgc caaaacggtt tctgtggatg cgggcgatat
    26581 cgcagcattt aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac
    26641 agagcctgct gtggacctct ttaaattact ttgagggatt catcatggaa atcagcctgt
    26701 tgcaggcatt tgcgttgggc attatcgcct ttatcgctgg cctagatatg tttaacggct
    26761 taacccatat gcaccgcccg gtggtcctcg gcccgttggt cgggctggta cttggcgatc
    26821 tgcataccgg aattttaacc ggcgatacgc tggaactagt gtggatgggg ctggccccgc
    26881 tggcgggcgc acagccgcct aacgtgatta tcggtactat cgtcggcacg gcgtttgcca
    26941 ttactactgg cgtgaaaccc gatgtcgcgg tcggtgtcgc cgtacctttc gctgtcgcag
    27001 tacagatggg gattaccttc ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc
    27061 ggatggcgga gaacgccgat actcgcggca ttgaacgcgt gaactatctg gcactgctgg
    27121 cactcggcac tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg
    27181 caaaaaccat cattgatgtc ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg
    27241 gcatcatgcc agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca
    27301 taccctactt catcctgggc tttgttgccg caacctggct caagttaccg gtgctggcta
    27361 ttgctgccgc cgcgctggcg atggcgctga tcgatctgct gcgtaaatct cctgaaccga
    27421 ctcaacctgc ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg
    27481 aacgtcagcg aaaatgaaga aacactgctg actggcgtca atgaaaacgt gtatgaagat
    27541 cggagcattg ccgcggagct gacgaaaaaa gatatcaacc gcgtcgcctg gcgttccatg
    27601 ctgttacaag cgtcttttaa ctacgaacgt atgcaggctt ctggctggtt gtacggtctg
    27661 ctgcctgctc tgaaaaagat ccacactaat aaacgcgacc tggcgcgcgc catgaagggg
    27721 catatgggtt tcttcaatac ccatccgttt ctggtgacgt ttgttatcgg cattatcctt
    27781 gcgatggagc gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt
    27841 gcgccgcttg gcgggattgg cgatgcgatg ttctggctaa cgctactgcc gatttgtggc
    27901 gggataggcg caagcctcgc gctacaaggc tctattcttg gcgcagtcgt ctttattgtg
    27961 ctgttcaacg tggtacactt ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg
    28021 ggcgtggcgg cgattcccct tattaaagcg aataccaaaa aagtcggcca tgctgcgtca
    28081 atcgttggaa tgacggtaat cggcgcactg gtggcaacct atgttcgttt aagcaccacg
    28141 ctggaaatca cggcgggcga cgcagtagtt aagttacagg ctgatgttat cgacaaactg
    28201 atgcctgcct tcttaccgct ggtctacacc ctgaccatgt tctggctggt acgccgcggc
    28261 tagagtccgc tgcgcctgat tgcggttacc gtggttctag ggatcgtcgg taaattctgc
    28321 catttccttt aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg
    28381 ggtttgccag tggcatggaa aaagcaatga agcaaatcct cggcgatcaa tcgcagttta
    28441 ttgccatcga ttttcccgaa acctcaagcg ccgcgctgct tacatcgcag cttgaagagg
    28501 ccatcgccca actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca
    28561 cgccgtttcg cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgattaccg
    28621 gcaccaattt gcaactgcta ctggagatgg tgctggagcg cgaagggtta agcggtgaag
    28681 aattccgtgt acaggcgctg gagtgtggac accgagggct gaccagtctg gtggacgagt
    28741 taggccgctg ccatgaagaa tgtccggtcg aggaaggaat atgacacacg ttctgcgcgc
    28801 cagaaggctg ctgactgaag agggatggtt cgatgaccat cagttgcgtg ttgctgacgg
    28861 tgtcatcgca gcaatcgaac cgattccagt gggcgtgact gaacgcgatg cggaactgct
    28921 ctgccccgct tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga
    28981 cgcgccggat gtactcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg
    29041 gttaccgacc accgtaaccg cgccgcttaa taccattcat gcggcgctga aacgtattgc
    29101 tcaacgttgc caacgcggcg gacctggtgc gcaagtgctg gggagttatc tcgaaggacc
    29161 gtacttcacg ccgcagaata aaggcgcgca tccg
//
LOCUS       DBKELA010000047        29049 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.047, whole genome shotgun sequence.
ACCESSION   DBKELA010000047 DBKELA010000000
VERSION     DBKELA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.047"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..89
                     /locus_tag="ACUQR0_002045"
     CDS             <1..89
                     /locus_tag="ACUQR0_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HHV9109088.1"
                     /translation="ANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            108..1139
                     /gene="mdtN"
                     /locus_tag="ACUQR0_002046"
     CDS             108..1139
                     /gene="mdtN"
                     /locus_tag="ACUQR0_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HHV9109089.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1139..3187
                     /gene="mdtO"
                     /locus_tag="ACUQR0_002047"
     CDS             1139..3187
                     /gene="mdtO"
                     /locus_tag="ACUQR0_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HHV9109090.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3184..4650
                     /gene="mdtP"
                     /locus_tag="ACUQR0_002048"
     CDS             3184..4650
                     /gene="mdtP"
                     /locus_tag="ACUQR0_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HHV9109091.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4848..6995
                     /gene="fdhF"
                     /locus_tag="ACUQR0_002049"
                     /pseudo
     CDS             4848..6995
                     /gene="fdhF"
                     /locus_tag="ACUQR0_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            7089..7778
                     /gene="yjcO"
                     /locus_tag="ACUQR0_002050"
     CDS             7089..7778
                     /gene="yjcO"
                     /locus_tag="ACUQR0_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HHV9109092.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="ACUQR0_002051"
     CDS             complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="ACUQR0_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HHV9109093.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="ACUQR0_002052"
     CDS             complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="ACUQR0_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HHV9109094.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="ACUQR0_002053"
     CDS             complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="ACUQR0_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HHV9109095.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10480..12198)
                     /locus_tag="ACUQR0_002054"
     CDS             complement(10480..12198)
                     /locus_tag="ACUQR0_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HHV9109096.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="ACUQR0_002055"
     CDS             complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="ACUQR0_002055"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HHV9109097.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="ACUQR0_002056"
     CDS             complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="ACUQR0_002056"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HHV9109098.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="ACUQR0_002057"
     CDS             complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="ACUQR0_002057"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HHV9109099.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="ACUQR0_002058"
     CDS             complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="ACUQR0_002058"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HHV9109100.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16279..18237
                     /gene="acs"
                     /locus_tag="ACUQR0_002059"
     CDS             16279..18237
                     /gene="acs"
                     /locus_tag="ACUQR0_002059"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HHV9109101.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18437..18751
                     /locus_tag="ACUQR0_002060"
     CDS             18437..18751
                     /locus_tag="ACUQR0_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HHV9109102.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18748..20397
                     /gene="actP"
                     /locus_tag="ACUQR0_002061"
     CDS             18748..20397
                     /gene="actP"
                     /locus_tag="ACUQR0_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HHV9109103.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20575..21867
                     /locus_tag="ACUQR0_002062"
     CDS             20575..21867
                     /locus_tag="ACUQR0_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HHV9109104.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22021..23670)
                     /locus_tag="ACUQR0_002063"
     CDS             complement(22021..23670)
                     /locus_tag="ACUQR0_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HHV9109105.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="ACUQR0_002064"
                     /gene_synonym="yjcD"
     CDS             complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="ACUQR0_002064"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HHV9109106.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="ACUQR0_002065"
     CDS             complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="ACUQR0_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HHV9109107.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26426..26749
                     /gene="soxS"
                     /locus_tag="ACUQR0_002066"
     CDS             26426..26749
                     /gene="soxS"
                     /locus_tag="ACUQR0_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HHV9109108.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="ACUQR0_002067"
                     /pseudo
     CDS             complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="ACUQR0_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28758..29039
                     /locus_tag="ACUQR0_002068"
     CDS             28758..29039
                     /locus_tag="ACUQR0_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HHV9109109.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7777 c         7350 g         6634 t
ORIGIN      
        1 gggcaaatat caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg
       61 ccatgctgtt ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc
      121 cgaaaaaagc acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg
      181 cccttgtttt cgttatctgg ctcgtagaca gtgcgccatc aactaatgac gcttacgcgt
      241 cagcagatac cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca
      301 ccgacaacca ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg
      361 aagccaacct ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca
      421 cccagcgtag cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa
      481 aagcccgtgc cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga
      541 aagagggttt tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag
      601 aagcggatct taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg
      661 tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac
      721 atctggaaat gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct
      781 ccgtcgggca atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact
      841 ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg
      901 caacgattcg cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg
      961 gctacggcgt gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc
     1021 gttctattaa ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac
     1081 ctgacccgga aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat
     1141 gagcgcgctc aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga
     1201 gttaagcgag cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct
     1261 gctggtgatt ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt
     1321 gctgttttac ggtatcggta ttcagtcgaa cgcgttttac accaaatttg tggcgatctt
     1381 gtttgtggtt gccacggtgc tggagatcgg cagcctgttt ttgatctaca aatggtcata
     1441 cggcgaaccg ttgatccgat tgatcatcgc cgggccgatc ctgatgggct gcatgttttt
     1501 gatgcgcacc catcggctgg gactggtctt tttcgctgtc gccatttacg ggcaaacctt
     1561 ccccgccatg ctcgactatc cggaagtagt cgtgcgctta acgctgtggt gtatcgttgt
     1621 tggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccaatcgtgc
     1681 cattacgcaa atgcatcaag cgcttaatga tcggcttgat gatgccatta gccacctgac
     1741 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
     1801 actgaatgtc ttttgtctcg cggacgatgc caactggcga actcaaagcg catggtggca
     1861 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
     1921 ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcttcag aaatcaacaa
     1981 gctgcagcat gccgttgctg aaggtcagtg ctggcaaagc gactggcgga tcagtgaaag
     2041 tgaagcggtg gcggcacggg aatgtaacct ggagaatatc tgccagacgt tgttacaact
     2101 gggccagatg aacccgaata cgccgccaac gcccgccgcc aaaccgccat caatggtcgc
     2161 cgatgctttt accaatccag actatatacg ctacgcggta aaaacattgc tcgcctgttt
     2221 gatctgttac accttttaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
     2281 ctgcgtgatc gtcgctaatc caaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
     2341 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgctactgg tcatgccctg
     2401 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tggccgcatg
     2461 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
     2521 gctcgccacg ctcgaaaacg tttttggccc agtgtacgac ctggtggaaa ttcgcgatcg
     2581 cgccctgggt atcatcattg gtaccgtggt gtccgcgatg atttacacct ttgtctggcc
     2641 tgaaagtgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttaagtaa
     2701 agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc tgcaaattcg
     2761 tatcggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga
     2821 gcgtcaactg gacagcgaag aacgcgcctt actgattgaa cgttcgcaaa cggttattcg
     2881 tcagggccgc gatatccttc acgcctggga tgccacctgg aattcggcgc aggcgctgga
     2941 taacgcacta cagccggaca gagcaggtca gtttgccgac gccctggaga aatacgctgc
     3001 cggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaga cacccacctc
     3061 gcaggccatc ctgcccacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
     3121 cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggcac aaggagccac
     3181 gcaatgatca atcgtcaact ttcacgtctg ctgttgtgca gcattctcgg cagcacgacg
     3241 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
     3301 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
     3361 tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt
     3421 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcaatcgca ggccgatttg
     3481 ttagatgccg gttcacagtt gcaggtggca gcgttaggga tgcttaaccg ccaacgtgtc
     3541 tcggcgaacg gctttttaag cccttatgcg atggatgcgc ccgcactggg gatggacggg
     3601 ccgtactata cggaagccac agtaggtttg tttgccggac tggatcttga tttgtggggt
     3661 gtgcatcgct cagcggttgc cgccgccatt ggcgcgcata atgccgcgct ggcagaaacc
     3721 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
     3781 agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta cgcggtgaaa
     3841 gcgcaccaaa gtaaagtggc gcacggtctg gaagcacaag tgcctttcca cggcgcgcgg
     3901 gcacagattc tggcggtcga taaacaaatt gctgccgtca aagggcaaat caccgaaacg
     3961 agagaatctc tgcgcgcatt gattggcgcg ggagccagcg atatgccgga gatcaaacct
     4021 gtggcattac cacgagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc
     4081 agacgcccgg atctgcaagc catgcgctgg tatgttcagg cgtcattaga tcaggtggat
     4141 tccgcgcggg cgttgttcta tccgagcttt gatatcaaag cgtttttcgg ccttgatgcc
     4201 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaacttcat cccgggtctg
     4261 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac gcgcgccgcc
     4321 agcaacatga tgattgaacg ttacaaccag tcagtactga acgcggtgcg tgacgttgcc
     4381 gtcaacggca cgcgtctgca aacgctcaac gacgagcgag aaatgcaggc tgaacacgtg
     4441 gaagcaacgc gcttcaccca gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc
     4501 cgcttacagg ccactgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg
     4561 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
     4621 caggcggctc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg
     4681 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
     4741 atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa gatgcatact
     4801 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
     4861 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
     4921 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
     4981 gttattatgg ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
     5041 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgagat gaggcactga
     5101 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
     5161 cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
     5221 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
     5281 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
     5341 ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg
     5401 cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
     5461 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
     5521 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac aaagcgttcg
     5581 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
     5641 agtcggttga agatatcacc ggtgtcagcg ccagtgagat tcgtcaggcg gcacggatgt
     5701 atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg
     5761 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gaccagtaac ctcggtaagc
     5821 cgcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
     5881 gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaaa
     5941 aattcgccaa agcctggggc gtggaaagcc tgcctgctca taccggctat cgcatcagcg
     6001 agctgccgca ccgcgcagcg catggcgaag tgcgtgccgc gtacattatg ggcgaagatc
     6061 cgctgcaaac tgatgcggag ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
     6121 tcatcgttca ggacatcttt ataaccaaaa ccgcgtcggc ggcggatgtc attttgccgt
     6181 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
     6241 tcttcaaagc agttgaaccg aaatgggatc tgaaaacgga ctggcaaatc atcagtgaaa
     6301 tcgccacccg tatgggttat ccgatgcact acaacaacac ccaggagatc tgggatgagt
     6361 tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc gaactgggct
     6421 tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct tatctgttta
     6481 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
     6541 caatcgacaa actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc
     6601 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
     6661 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
     6721 tggtttgagt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
     6781 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
     6841 ttaccgaaag cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
     6901 tcgagccgat ctccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
     6961 tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt
     7021 cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta
     7081 ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga
     7141 acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggggac cgacgtgcac
     7201 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
     7261 cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttggcg cagatcaaaa
     7321 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc
     7381 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat actcaagcgg
     7441 gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcctcggaa gatctggaga
     7501 atgactccgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
     7561 ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgca atttcccgaa
     7621 ccggttattc cgagtactgg gcgggaatga tgttcttaaa cggtgaggaa ggttttatcg
     7681 agaagaacaa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa gggtttgata
     7741 ccgggtgtga agagtttgaa aaattaacca acggttaagg taggcctgat aagacgcggc
     7801 aagcgtcgca tcaggcatcc acacattgcc gggggatatc cccggcaatc ttcaattatt
     7861 gatccgcagt tttatcaaat ttgcccagca cttcacgctc ataagccagc gcctttttgc
     7921 ggtcaaattt gtgttcccac ttggcaatca ccagcaccgc cagcgcatta cccaccacgt
     7981 tcagcgcagt acgcgccatg tcgaggatac ggtcaacacc agcaataaac gccagacctt
     8041 ccagcggaat acctacgcta cccagcgttg ccagcaacac cacaaacgac acgccagcca
     8101 cgccagcaat ccctttcgag gtcaccatca gcgtcagcac cagaatgatt tcctgccaga
     8161 tggacaggtc aatgccatac agctgagcga tgaaaatagc ggcaatactt tgatacagcg
     8221 tcgaaccatc aaggttaaaa gagtaaccgg tcggcaccac gaagctggtg atcgacgccg
     8281 gtgctccgta ggcttccatc ttctcaataa ttcgcggcag tacgctttca gagctggcag
     8341 tggagtacgc cagaatcagc tcatctttca gaatacgaat caggatccag acgcttaacc
     8401 cgcacagacg tgccacaatt cccagcacta ccagcgcgaa gaacagaatg gcgaaatgca
     8461 ccagcagcac cagtttcgcc agtggccaca gagacgagaa accaaagtta gccaccgtca
     8521 ccgcaatcag cgcaaacaca cccaccggtg cataacgcat caccatgtga gtcactttaa
     8581 acatggtttc agagatggag cggaacacgg tcaccagcgg ttcacgatgc gtcgcgggca
     8641 gggaagaaag ccccagacca aacagcaccg agaaaaagat gatcggcagc atttcgcctt
     8701 tcgccatcga cgccacaatg ttcgtcggca ccagcgacaa aatcgtgccc ataatgccgt
     8761 gggaactgct ttgtaccgct tccgtagtgc tctgatattt cgagatatcg acggtcgcca
     8821 gctgcgacat atccaccccg gcaccgggct ggaagacgtt cgccagagtg atccccaaaa
     8881 tgatggcgac ggtggtgatc acctcgaagt aaataatggt tttcgcgcca atacgcccga
     8941 gctgtttggc atcaccaaca cccgcgatac ccaccaccag cgtggagatc acaatcggca
     9001 caacaatcat tttgatcaga tggatgaaga tatcacccgc cggagagagc aaattgacga
     9061 ccagccagtc gcggctgtcg ctatggtagt gcaggtagct tcccaggaga atgcccagca
     9121 ccatagcaaa cagaatctgc caggccaggc tgaattttat atttttcatc agaataactt
     9181 cctcaatgga gcgccgttcc ccgttcagaa acgcgagaac gtgacatact gccagggcat
     9241 gaattgtgtt gcgttggttt gtagattttt ttaacgctta tgatcagtat catttgcgcg
     9301 gtatgttgcg caacccttta acaacagagc ttttcgctgc attaatgcat aataacgcga
     9361 gtttatggca ggattaatga ataaccattt attatggaaa gatttttatt tgtttaatct
     9421 gcaataatac tgattggtga ttatttttgg catatgttca ttcgattatt ttttatgcag
     9481 ctcaatgaca taatcttcat caccagatga ttaatccgat ctccgctgca acagttttgc
     9541 catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga gatccatcac
     9601 tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat cggaagccag
     9661 cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg
     9721 agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca gcgccgcata
     9781 cagttcagcg ttctcaccac gtagttgcaa cgcctgacga tacgccagca gcgaattgct
     9841 gtaatcgttt tgccacagat agtactcgcc cagtaacgcc cattgttcgc tgttttgtgg
     9901 attagcgcgg attttatcct gcaatgcctg aagctgcgct tccggggttt gctggctggc
     9961 aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct gccattttgg
    10021 gcttaacaga taactgccga cacagaggaa taccatcaat atcgtgagtg ttgtcagcat
    10081 tttcaccggt atttgaggct gtttcatcgc ttagccctca ctcgccagag gatcagtgcc
    10141 atcagcaata acaacaccac tggcagcgcc cacaacacca gcgtctgacc cgttaacggt
    10201 gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg
    10261 ttcttcccct ccgacaccat gctataaacc tgatggcgca tactgaccgc caccggcgca
    10321 ttggattcga gtaagttttg attctggcac tgcggacaac gtaactggct ggcaatattt
    10381 aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacaac ctgagcgcgc
    10441 gcaaaacagg taaataacag cagtagcgtg agaagccctt tattcatcgc gcttcttccc
    10501 ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc agatccagcg
    10561 cacaccgctt tgtacataca aacgaaaagc gtaacgatcc gctctggttt tttcacccat
    10621 gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca tttgctgacg
    10681 gcgtgcttca taaaaacgcc gctccggcgt taattcacca atgcgttgct gatggtcaaa
    10741 cagcgccact atcgcttttt cgctggtgta attgcctctg gcttgcagat cgagtcgctc
    10801 aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat
    10861 ctcctgacga ctgacactgg aaaccacgat ccccgcggca aataacagca cgccagcatg
    10921 agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca gcacaatcac
    10981 caccagcatc aacagaccaa acggtaacgt cgcgcggtta aaatacggcg cgccaacgga
    11041 gaggcgtccc cagcccagca ggccgtaaat catcggataa agcgttccca ccagcacgat
    11101 cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc gcgataaccc
    11161 cgaaaaacgc accgccgggc caccgtcccg cgctcgccag ccatacagag ccaaagacgc
    11221 aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa
    11281 cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtgcccagca gcgacagcat
    11341 cagagtaact atcgccagca acagcgacca gtggcggaaa atcccccgct ggcgtgtcag
    11401 ggacaaactg tgcagcagcg cagtggcaga aagccagggt aataaagagg cgttttccac
    11461 cggatcccag aaccaccagc cgccccagcc gagttcgcag taggcccacc aggaaccgag
    11521 gatgatcccc gccgttaatg cactccagcc aggtaacgcc cagcgccagc aaattcgggc
    11581 gctggccgca tcaaactcgc cgcgcagtag actcgccagc gccacgctcg ccgctaccat
    11641 caaaccgcca tagccaaggc aaagcagcgg tggatgaaag ataagaccgg gatgttgcag
    11701 catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga agggatcgga
    11761 ccacagcacc acaaacagta gcagtgcggc gagcatgaga gataaaacgg ctagcgtcag
    11821 cggaaatagc ggatcgcttt gctgccgata atgccaggca aacagcgcgc tccaggcgga
    11881 aagcagcagc acccaaagca gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt
    11941 gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg agaaatcgct
    12001 gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga actgcgccag
    12061 aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc
    12121 cgtcaacggg gtcaacacgt tgaccccgag acttaacaac aacgccagaa aaccgacttc
    12181 aggaaggaat acgtccagaa accacctctt ttctggctta cgccaccgtc aactgtccgg
    12241 cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag actggcccca cacgccgcca
    12301 gcacggcagg aatgccggaa ctgcgattga cccacggttt gagcaacatt ggcacaatca
    12361 gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca cctaatgccg
    12421 ccaccagcgc acgaacttta ccgtcatcgc ccagcgccag accgacaaaa aacgccacca
    12481 gcaggaagat ttcaccccat accacgggga tttccatccg gtgaacaaag tgcgcttccg
    12541 tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg
    12601 acgagatgcc ggagaagagg agcagcaccg gcaggctcgg gttattgagg aacggatacg
    12661 atttcagcgc cgacagcaga aagccggtat aagcccccag cagcaccgcc aacaccagca
    12721 tcagcgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc
    12781 ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag attttcgcca
    12841 gccacagcac cagcaccacc atgtagagct gaaacagcat cacccccatc gacatcaccg
    12901 aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc aggtggaaga
    12961 cgaggatcaa cagaccgagg atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca
    13021 acgtactgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcacg gccagcgtca
    13081 ccagaccggc agaaatacca atcaaaaaca ggtagatggc aatcggccag tcccacacca
    13141 gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt atttaaacgg
    13201 tacgcggtac agcttcggtt tggttcccag cgccagcttg tagcggtaag taggcttctg
    13261 gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg tcagcgcctt
    13321 cgtcgggcaa gcttcaacgc acgcgggaag cttaccggcc tgcaaattgg tcttacgaca
    13381 gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc ggtacggaca
    13441 ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga caatcccgct
    13501 agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggca catgatcgca
    13561 gtgctggcaa gacttacgga agaagcgata tttcacatca ggaaattcgc cctgcggctc
    13621 gctacgaata atcgtcaggc gcgagacgcc ttccggcacc ttgttcactt cccgacaagc
    13681 atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac
    13741 cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga cggctgccag
    13801 cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc cttaagcaac
    13861 ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca atcgacgcaa
    13921 atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa
    13981 tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa cgctttttgc
    14041 aactgttccg gcagatgaca ggacatacag acgctgttct gctctccgac cttatacatc
    14101 ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga tgcttggccg
    14161 tgacagttgg tgcaggtgac cggcaatttg ttattcgggt tgataacgga agcatgtttg
    14221 ccatgcatac cttcggtatc tggtttatga cagtccagac aggcggcatc cgggttacgc
    14281 tgctgggtaa cttcgtagcg atcaccagat gcctgcaccg caggggtagc ggtaattccg
    14341 ttcaggctcc acaacaggcc cgacgccagc accccagcag ttaataacga acgtaatacg
    14401 ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt tattggctta
    14461 acagaccgtt tttacgtgcc tgctcttccc actgcgggat caccgttttg atgaagtcct
    14521 gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct ttctctttgg
    14581 tcgagatatc cgggatctgg atttcatggg tgataccttt ggtcgccagc aggcgcgcca
    14641 gtttggtgcg cgcatccgcc gctttatcca tcgccgtacc gagcatccgt agaccttctt
    14701 ccggtgcgtg catatgaata ccgtgggaag cgatcgccag atcccaacgc cactgggcat
    14761 gacggatatc gtcctgaatc ggcttcattt cggcttccgt cgcgcctgca tccagcgccg
    14821 ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc aggtcgttaa
    14881 tcgattgctt acgttccgcg accacttttt gcaaggcagc tttatcctgg gtatggcagt
    14941 tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg gtgtagagtt
    15001 tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg ttgtttttac
    15061 cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc attggcgttt
    15121 tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc tccatatttt
    15181 cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg tcgaagtaat
    15241 actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg gctttctcaa
    15301 aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg gttaactccg
    15361 gtttgccttt ggcgaactct ggtgaggcgg tgttatggca gtcggcacaa cctaagttgt
    15421 tgacgatttc cgggccgccg cgcgcccatt taccgtggaa gtagccatct tcgccgtctt
    15481 tctggatcag acgcgccaca tccgggcttt tacaactcca gcacgccatc ggtagcggac
    15541 catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg gtcacagcaa
    15601 aggcatgtcc acgaggcttg ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca
    15661 ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag gtggctttcc
    15721 aggagagata ttgatcgtga tgctgcgggg caaaggtttc attcttcgct tccacagtta
    15781 caggttttgc gggagctgcc gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa
    15841 tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg gcttcatccg
    15901 aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa ccgcacctac
    15961 actgtcatga cattgctcac ccctatgtgt aacaaataac cacactgtga atgttgtctt
    16021 taatcaattg taagtgcatg tgaaatacca ctttagagtt agtcagtatc ttcctctttt
    16081 tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt tggtgacctt
    16141 caaaaattac cctgccgttt atttgcacca ttctactttt gcgtgatctg tcgcccaaat
    16201 actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt attaacatcc
    16261 tacaaggaga acaaaagcat gagccaaatt cacaaacaca ccattcctgc caacatcgca
    16321 gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc tattaacgta
    16381 cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc gtaccagaag
    16441 gtgaaaaata cctcctttgc ccccggtaat gtgtccatta aatggtacga ggacggcacg
    16501 ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga tcgcaccgcc
    16561 atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa agagctgcac
    16621 cgcgacgtct gccgcttcgc caataccctg ctcgagctgg gcattaaaaa aggtgatgtg
    16681 gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc ctgcgcccgc
    16741 attggcgcga tgcattcggt gattttcggc ggcttctcgc cggaagccgt tgccgggcgc
    16801 attattgatt ccaactcacg actggtgatc acttccgacg aaggcgtgcg cgccgggcgt
    16861 agtattccgc tgaagaaaaa cgttgatgac gcactaaaaa acccgaacgt caccagcgta
    16921 gagcatgtgg tggtactgaa gcgtactggc ggaaacatcg actggcagga agggcgcgac
    16981 ctgtggtggc acgacctggt tgagcaagcc agcgatcagc accaggcgga agagatgaac
    17041 gccgaagatc cgctgtttat tctctatacc tccggttcta ccggaaaacc aaaaggcgta
    17101 ctgcacacta ccggcggtta tctggtgtac gcggcgctga cctttaaata tgtctttgat
    17161 tatcatccgg gtgatatcta ctggtgcacc gccgatgtgg gctgggttac cggacacagt
    17221 tatttgctgt acggcccgct ggcctgcggc gcgaccacgc tgatgtttga aggcgtaccg
    17281 aactggccga cgcctgcccg tatggcgcag gtggtggaca agcatcaggt caatattctc
    17341 tataccgcac ccacggcgat ccgcgcgctg atggcggaag gcgataaagc gattgaaggc
    17401 accgaccgtt cgtcgctacg cattctcggt tccgtgggcg agccaattaa cccggaagcg
    17461 tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga tacctggtgg
    17521 cagaccgaaa ccggcggttt catgatcacc ccgctgcctg gcgctaccga gctgaaagcc
    17581 ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac
    17641 ccgctggaag gggctaccga aggtagcctg gtgatcaccg actcctggcc tggtcaggcg
    17701 cgtacgctgt ttggcgatca cgaacgtttt gagcagacct atttttccac cttcaaaaat
    17761 atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg gatcaccggg
    17821 cgtgtggacg acgtgctgaa cgtctccggt caccgtctgg ggacggcaga gatagagtcg
    17881 gcgctggtgg cgcatccgaa gattgccgaa gccgccgtag taggtattcc gcacaatatt
    17941 aaaggtcagg cgatctacgc ctatgtcacg cttaatcacg gggaggaacc gtcaccagaa
    18001 ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc gacgccagac
    18061 gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat gcgccgtatt
    18121 ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac gcttgccgat
    18181 cctggcgtgg tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc
    18241 acaattgccg gatgcgaatc ggtaacggtt tgtaggcctg ataagacgcg acagcgtcgc
    18301 atcaggcatt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaaggg
    18361 ttacccgagc gttaaccttc tcccataagg gagcgggaat taaaacaatc cctacattac
    18421 ctctggagaa tctgtgatga atggtacgat ttatcagcgg atagaagaca atgcgcattt
    18481 cagggagtta gtcgaaaaac ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc
    18541 agtttatatc ggctttattt tactgatcgc cttcgcgccc ggctggctgg gcacgccgct
    18601 gaatccgaac accagcgtca cacgcggtat tccaattggt gttggagtga ttgtgatctc
    18661 ctttgttctc accggtatct acatctggcg ggcgaacggc gaattcgacc gtcttaataa
    18721 tgaagtcctg catgaggtac aagcatcatg aaaagagttc tgacggcgct tgccgccaca
    18781 ctccctttcg cagctaacgc cgcggatgct attagcgggg ccgtagagcg ccagccaacg
    18841 aactggcagg cgattattat gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac
    18901 tgggcatcaa aacgcgtacg ttctcgtagc gactactaca ccgcaggcgg caatatcact
    18961 ggcttccaga acgggctggc gattgccggg gactatatgt ccgccgcctc attcttgggg
    19021 atctccgcgc tggtgtttac ctccggctat gacgggctga tctactcgtt gggcttcctg
    19081 gtgggctggc caatcattct gtttctgatt gccgaacgtc tgcgtaacct gggacgctac
    19141 acctctgccg atgtggcctc ttatcgtctg aaacaagggc cgattcgtat tctttcggcc
    19201 tgtggttctc tggtagtggt ggcgctttac cttatcgctc agatggtagg cgcaggtaaa
    19261 ctgatcgagc tgctgtttgg ccttaactat cacattgcgg tggtgctggt cggcgtgctg
    19321 atgatgatgt acgtcctgtt cggcggcatg ctggcgacca cctgggtaca aattatcaaa
    19381 gctgtgctgt tgctgttcgg tgccagcttt atggccttta tggtgatgaa acacgtcggc
    19441 tttagcttca acaatctgtt cagcgaagcg atggcggtac acccgaaagg tgtcgacatc
    19501 atgaaaccgg gcggactggt gaaagatccg atctccgcgc tctctctggg gctgggactg
    19561 atgtttggta cggcgggctt gccgcacatt ctgatgcgct tctttacagt cagcgatgcc
    19621 cgcgaagcac gtaagagcgt gttctacgcc accggattta tgggctactt ctatattctg
    19681 acctttatta tcggcttcgg cgcgatcatg cttgttggtg cgaatccgga atataaagac
    19741 gcggcgggcc atctgattgg tggtaacaac atggcggccg ttcacctggc gaatgcagtg
    19801 ggcggcaacc tattcctcgg ttttatttca gcggttgctt tcgccactat cctcgcggtg
    19861 gttgcggatc tgacactggc gggcgcatcg gcggtttcgc atgacttgta cgctaacgtc
    19921 ttcaaaaaag gcgcgaccga acgtgaagag ctgcgggtat caaaaatcac cgtactgatc
    19981 ctcggcgtga ttgcgattat cctcggcgtg ctgtttgaga atcagaacat cgcctttatg
    20041 gtaggccttg cgtttgccat cgcggcgagc tgtaacttcc cgatcattct gctttccatg
    20101 tactggtcga aactgactac ccgtggcgcg atgctgggcg gctggctggg gctgattacc
    20161 gcagtggtgc tgatgatcct cggcccgact atttgggtac agatcctcgg tcacgaaaaa
    20221 gccatcttcc cgtatgaata cccggcgctg ttctctatca gcgtggcatt cctcggcatc
    20281 tggttcttct cggcaaccga taactcagcg gaaggggcgc gcgagcgtga actgttccgc
    20341 gcgcagttta tccgctccca gaccggcttt ggcgttgagc aaggccgcgc acattaatct
    20401 ctccccttcc ccggtcgcct gaccggggaa tacgcttcct ctccaacatg catcaccttt
    20461 tcccataata ttaaacaaat aaactcatta aaaaatgagc gatttttgac agtcgtagaa
    20521 aatgataatg cagagaatat gccttttctt tcttgttaat tataaggata ttttatgcgc
    20581 tataatggtt taaataatat gtttttccct ctttgccaga ttaacgataa ccactctatc
    20641 acaagttcat cacatacaaa gaaaacaaaa tcagataatt acagcaaaca tcataaaaac
    20701 acgttaattg acaataaagc cctctctctt ttcaaaatag atgatcatga aaaagtgata
    20761 ggcttgattc agaaaatgaa aagaatttat gatagtttac catcaggaaa aatcacgaaa
    20821 gaaacggaca ggaaaataca taaatatttt atagatatag cgttatatgc aaataataaa
    20881 tgtgacgata gaattacgag aagagtttac cttagtaaag aaaaggatgt atccattaag
    20941 gtggtatatt ttataaataa tgtcgccgtc cataataata ctatcgaaat tccacagata
    21001 gtaaatggtg gttacgattt ttcacacctt agcctgaagg gtatcgtgat taaagatgaa
    21061 gatttatcca attcgaattt tgcaggttgc agactacaaa acgctatttt ccaggactgt
    21121 aatatgtata aaacgaattt ttattacgcc ataatggaaa aaatactttt tgataattgt
    21181 attctcgatg actcaaattt cgctcagata aaaatggccg acggaactct aaatgcatgc
    21241 tccgctatgc atgttcaatt ctacaatgca gcaatgaata gagccaatat taaaaatacc
    21301 ttccttgact attcaaattt ttatatggcg tacatggctg aggtaaatct ttataaagta
    21361 atagcgccat atgttaattt atttaaagcc gaccttagtt tctctaaact cgatttaatt
    21421 aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaatcct ccagaatata
    21481 aacttaattg atagcaaact cttttgtacg tggctgacaa atacattcct cgaaatggtt
    21541 atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt aagcaactgc
    21601 catttcaact gttctatttt aacaaaagcc tgtatgttta atacccgtct ctatcgggtt
    21661 aatttcgatg aggctagcgt ccagggaatg ggcatttcca ttctccgtgg ggaggaaaat
    21721 atccccattg atagtgatac cctggtaaca ctacagaaat tctttgaaga agattgtacc
    21781 tctcatactg gcatgtcaca aactgaggat aatattaatg cagtcgctat gaagattact
    21841 gcagatatta tgcaacacgc agattgaggt taagtcgaag aacagccaat gaattgactt
    21901 tacaagagag ataacttatt tgttttagaa tcatgaatgt tcgcccgagc taacctgtta
    21961 aaggtgcaaa agattgcacc tttttcagat tacctcgggc ctggttacgt cgctttcggc
    22021 ttactgattt tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg
    22081 cagcgtttcg ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga
    22141 acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac
    22201 gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt
    22261 aagcaactgg ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg
    22321 gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttctt ctttcaattg
    22381 ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac
    22441 aaacagcgag aagagaatga cgccagccgc caggaacacc agctcatagc gcgccgggaa
    22501 gacgttacca tccggcaaga gcagcgggat tgagagcaca ccggccagag tgatcgcccc
    22561 acgcacccca gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat
    22621 cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg
    22681 gaccagcatc agcgccgcat atatcagaat aatatcggta aacagcatcc agatttcgac
    22741 gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca gttgcagacc
    22801 taacagcagg aacaccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc
    22861 gcgcaggcgc attgccagtg gcgcacggcg catcacaccg gagcgggtga tggtcatccc
    22921 ggcggcaacc gcagcgagga tgcccgaaac gccaatatgt tcggcaatca gataagaagc
    22981 gaatggcagc aagaacagca gaacgatctg cgtcgcgggt tcatcaccgc cccagcggct
    23041 gaggaatcgc agcgagcgac catacagcca gctcaccaca aaaccggcaa gaataccgcc
    23101 aatggctact ttcataaatt cgaccgtcgc accgccgaca gtgaagatca tcgtccccat
    23161 cgccactgcc acggcaaact tcaacgacac caggccggaa gcgtcattca tcaacgcttc
    23221 gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa tcccggagag
    23281 cgccacagca tcggtcggag aaagtaccgc cgccagcgca aaggcgggga tcagcggaat
    23341 gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc
    23401 cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca
    23461 gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc
    23521 cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg
    23581 aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt
    23641 cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat
    23701 ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
    23761 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
    23821 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
    23881 gatgttcaac ttgcgccatt caccggaaac cagacgacca atcaccagag tcgcgaagcc
    23941 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
    24001 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
    24061 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag aaagcggaga
    24121 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc cggttttacc
    24181 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
    24241 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccgccgt cgatgatctg
    24301 cccatcttta tccagcaggt tcgcctggcc ggcgacagcg cggatagttc cggtggcatc
    24361 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
    24421 cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
    24481 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
    24541 aatggtcagc agaatgccac cagggacttt cagtttttcc aagccgatga tcaccgccag
    24601 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
    24661 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag
    24721 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
    24781 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
    24841 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa acgccgtcag
    24901 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
    24961 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
    25021 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
    25081 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
    25141 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
    25201 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
    25261 gcggcaattt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
    25321 gtaaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
    25381 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
    25441 cgtcgatgtg caagattgca gcgttatcga gccgtatacc gttctggatt gcgataaaaa
    25501 atcgtctgag atgacaaaaa tattgcggct aaaaagtata cttttacact ggcgaagaaa
    25561 cgtcggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
    25621 gaggtgtgtt gacgtcgggg gaaaccctcc tgtgttccag cgggatagag agaaagacaa
    25681 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgtgttt gcggataatt
    25741 ccaaaagagc tcgatcagtc ccctcgcccc ttcggggaga gggttagggt taggggaaca
    25801 ggcagcacca gtgcaaatat ttaccctcac cccggcccca tagttaacga tgagtcagct
    25861 tgtccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg taccttcttc
    25921 tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc
    25981 aatacatccg tccagttcgt cacgcagcgc cactaaggta tgaatgcgcc gatccaactc
    26041 ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat gcccttctgg
    26101 caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct gagcgatttt
    26161 gataattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc cgctgttacg
    26221 gatactagta atcaaccctt tgctttcata gaaatgcagc gccgataccg ccacaccgct
    26281 gcgtttcgcc acttcgccag gggttagcag cgctttaatg cggggtaatt tcttttccat
    26341 aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
    26401 ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg atcttatcgc
    26461 atggattgac gtgcatattg accagccgct taacattgat gtcgtcgcaa aaaaatcagg
    26521 ctattcaaag tggtacttgc aacggatgtt ccgcacggtg acgcatcaga cgcttggcga
    26581 ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc
    26641 gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct cccgcgtttt
    26701 ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt tttattgccc
    26761 gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct gcggcggcat
    26821 cgccttcgca aaaaaccatc cctggcaata gcgcacgccg cgtttgcgca gccagttaac
    26881 ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt gcgccagctc
    26941 gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt caacaaacga
    27001 tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt tagagtagcc
    27061 aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca gaattaatcg
    27121 gcgtcatttt ttcgacatca agaaatgcat gctcagtcac ttcaaattta atttgctgcg
    27181 gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa
    27241 aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc aggtacgcgc
    27301 ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc atcccctcct
    27361 tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca ccaggccaac
    27421 gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt ggctggtaat
    27481 aaagacaaag ttgatgtttt tcgagggcgc gttgcaattt acgtttggga gataaatagt
    27541 tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata ccggcgggca
    27601 acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa gtcgcaacaa
    27661 tggctgcaat tgggcgtttt gtcgaataaa ttgtcgcata taaatagcca tttctttgta
    27721 cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc tctttgctta
    27781 acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc gggtcatcag
    27841 acatcacttc actccagaag agagggttga taaccgccac ataatttccc cgctgcatat
    27901 aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata gaaacgttag
    27961 gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct atcagcgatg
    28021 agcataaaaa atggttatca cgaattcatt aatataaaga tagccacgaa taatattcaa
    28081 cattcgcttt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag ctgcatcgct
    28141 cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt gagcaaaggt
    28201 tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata gcgaaagaat
    28261 gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt gtcgtgcacg
    28321 atgactcatt gctatcaatt tcctgcctgt ttatacccag ccattgcaag gtgaaaccct
    28381 ggctaccgac gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa
    28441 ttgatacctt tttaatcgtc acctggatga aaataattca ttgatattca aaattaattt
    28501 tctacaggaa tacagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata
    28561 atttgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata
    28621 gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga
    28681 tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt
    28741 ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac
    28801 ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg
    28861 attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg
    28921 caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt
    28981 cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat
    29041 acgtagaag
//
LOCUS       DBKELA010000048        28993 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.048, whole genome shotgun sequence.
ACCESSION   DBKELA010000048 DBKELA010000000
VERSION     DBKELA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.048"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            64..312
                     /gene="hybG"
                     /locus_tag="ACUQR0_002069"
     CDS             64..312
                     /gene="hybG"
                     /locus_tag="ACUQR0_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HHV9109110.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(435..1301)
                     /gene="yghU"
                     /locus_tag="ACUQR0_002070"
     CDS             complement(435..1301)
                     /gene="yghU"
                     /locus_tag="ACUQR0_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HHV9109111.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHELHDAS
                     DFETNTEDKRQG"
     gene            1506..3365
                     /gene="gss"
                     /locus_tag="ACUQR0_002071"
     CDS             1506..3365
                     /gene="gss"
                     /locus_tag="ACUQR0_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HHV9109112.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            3657..5156
                     /gene="pitB"
                     /locus_tag="ACUQR0_002072"
     CDS             3657..5156
                     /gene="pitB"
                     /locus_tag="ACUQR0_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HHV9109113.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(5205..5897)
                     /locus_tag="ACUQR0_002073"
     CDS             complement(5205..5897)
                     /locus_tag="ACUQR0_002073"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHV9109114.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            6071..6784
                     /locus_tag="ACUQR0_002074"
     CDS             6071..6784
                     /locus_tag="ACUQR0_002074"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHV9109115.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            6816..7574
                     /locus_tag="ACUQR0_002075"
     CDS             6816..7574
                     /locus_tag="ACUQR0_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109116.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            7620..8969
                     /locus_tag="ACUQR0_002076"
     CDS             7620..8969
                     /locus_tag="ACUQR0_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HHV9109117.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            8969..9793
                     /locus_tag="ACUQR0_002077"
     CDS             8969..9793
                     /locus_tag="ACUQR0_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109118.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            9805..10365
                     /locus_tag="ACUQR0_002078"
     CDS             9805..10365
                     /locus_tag="ACUQR0_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HHV9109119.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            10396..11466
                     /gene="lptF"
                     /locus_tag="ACUQR0_002079"
     CDS             10396..11466
                     /gene="lptF"
                     /locus_tag="ACUQR0_002079"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HHV9109120.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            11463..12542
                     /gene="lptG"
                     /locus_tag="ACUQR0_002080"
     CDS             11463..12542
                     /gene="lptG"
                     /locus_tag="ACUQR0_002080"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HHV9109121.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(12578..13749)
                     /gene="spt"
                     /locus_tag="ACUQR0_002081"
                     /pseudo
     CDS             complement(12578..13749)
                     /gene="spt"
                     /locus_tag="ACUQR0_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004758 - serine C-palmitoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046513 - ceramide biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine palmitoyltransferase"
     gene            complement(13749..13997)
                     /locus_tag="ACUQR0_002082"
     CDS             complement(13749..13997)
                     /locus_tag="ACUQR0_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHV9109122.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(14030..14944)
                     /locus_tag="ACUQR0_002083"
     CDS             complement(14030..14944)
                     /locus_tag="ACUQR0_002083"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HHV9109123.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(14941..16629)
                     /locus_tag="ACUQR0_002084"
     CDS             complement(14941..16629)
                     /locus_tag="ACUQR0_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HHV9109124.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            17033..18174
                     /gene="yghO"
                     /locus_tag="ACUQR0_002085"
                     /pseudo
     CDS             17033..18174
                     /gene="yghO"
                     /locus_tag="ACUQR0_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(18298..19068)
                     /gene="glcC"
                     /locus_tag="ACUQR0_002086"
     CDS             complement(18298..19068)
                     /gene="glcC"
                     /locus_tag="ACUQR0_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HHV9109125.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            19319..20818
                     /gene="glcD"
                     /locus_tag="ACUQR0_002087"
                     /pseudo
     CDS             19319..20818
                     /gene="glcD"
                     /locus_tag="ACUQR0_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            20818..21870
                     /gene="glcE"
                     /locus_tag="ACUQR0_002088"
     CDS             20818..21870
                     /gene="glcE"
                     /locus_tag="ACUQR0_002088"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HHV9109126.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            21881..23100
                     /gene="glcF"
                     /locus_tag="ACUQR0_002089"
                     /pseudo
     CDS             21881..23100
                     /gene="glcF"
                     /locus_tag="ACUQR0_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            23105..23509
                     /locus_tag="ACUQR0_002090"
     CDS             23105..23509
                     /locus_tag="ACUQR0_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HHV9109127.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            23531..25702
                     /gene="glcB"
                     /locus_tag="ACUQR0_002091"
     CDS             23531..25702
                     /gene="glcB"
                     /locus_tag="ACUQR0_002091"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HHV9109128.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            26057..27652
                     /locus_tag="ACUQR0_002092"
     CDS             26057..27652
                     /locus_tag="ACUQR0_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HHV9109129.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         7033 a         7014 c         7671 g         7275 t
ORIGIN      
        1 gggaacaccc gctaaaggtt ctttttcgcg tttgtccggc aatcgaaata accggagtta
       61 acaatgtgta ttggcgttcc aggccaggtg ctggctgtcg gtgaagatat tcaccagctt
      121 gcgcaggttg aagtatgtgg tatcaagcgc gacgtgaata tcgccctgat ttgcgaaggt
      181 aaccctgccg atctgctggg ccagtgggtg ctggtacacg tcggatttgc catgagcatc
      241 atcgacgaag atgaagccaa agccacatta gacgcactgc gccaaatgga ttacgacatt
      301 accagcgcgt gatgattagc ttttgtaggc ctgataagac gcggcagcgt cgcatcaggc
      361 attgtgcacg attgccggat gcggcgtgaa cgccttatcc ggcctggatg gcttgctgcg
      421 acgaacacca acccttaccc ctgacgctta tcttctgtat ttgtctcaaa atcactggcg
      481 tcatggagct catgcaactg ctcgttcagc ggcccgttgg tgcggttaac aatacgccca
      541 cgtttcactg ccggacgttc gcccacttct ttcgcccagc gttgcacatg tttataactt
      601 cccgcatcca gaaactctgc ggcatcatac acgccgccga gcaccacgtt gccaaaccac
      661 ggccaaatcg ccatatccgc aatggtgtac tcatcgcccg caacaaactt atgctgcgcc
      721 agttgcttat ccagcacgtc gagcaggcgc ttggcttcca tggtaaagcg gttgatggcg
      781 tactcaattt tcaccggcgc gtaatggtaa aagtgaccaa aaccaccgcc gaggaacggc
      841 gcggcaccct gtaaccagaa cagccagttc aacgtttcag tccgttttgc cagatcctgc
      901 ggcaggaagt agccaaattt ctccgccaga taaagcagga tcgaaccaga ttcaaacacg
      961 cggatcggcg gattatgcgt atgatcgcgc agcgccggga tcttcgagtt tgggttcact
     1021 tcgacaaagc cgctggagaa ttgatcgcca tcgccaatac gaatcagcca ggcgtcgtac
     1081 tctgcaccag taacgcccag cgccagtagc tcctcaagca taatcgttac tttctgaccg
     1141 ttcggcgttc ccagcgaata aagttgcaat ggatgtttgc caacgggcag cgttttttca
     1201 tgcgtcggac cagaaaacgg gcgattgata ttggcgaacg cgccgccagc ggatttatcc
     1261 cacgtccaga ctttcgcggg ctgataagta ttgtctgtca tagtgagttg ccttctgagt
     1321 ggtagtgttg aagcagtgta gcaggtcact tgctgtacag aatgtgaatt ttttaaccct
     1381 ttgcgccagc cgctgcgatg aggtgtatga ttagtctgac ctctgtccgg ttaatgtcgc
     1441 tgagcaggta atcagagcac aacaattacg ccaactgctt aggttgtatt gttaaaggta
     1501 aagtgatgag caaaggaacg accagccagg atgccccgtt cgggacatta ttgggctacg
     1561 ccccaggtgg ggtagcaatc tactcttcag attacagttc tctcgatccg caggaatacg
     1621 aagatgacgc cgtattccgt agctatatcg acgacgaata tatgggccac aagtggcaat
     1681 gcgttgaatt tgctcgccgt tttctctttc tgaattacgg tgtggtcttt actgacgtgg
     1741 gtatggcgtg ggagattttc tcgctgcgct tcctgcgaga agtggttaat gacaacatcc
     1801 tgccattgca ggcatttcct aacggctcgc cgcgtgcgcc ggtcgcgggt gcgcttctta
     1861 tctgggataa aggcggtgaa tttaaagaca ctggccatgt cgccatcatt acccaattgc
     1921 atggcaacaa agtccgtatt gcggaacaga acgtgattca ttccccgttg ccgcaagggc
     1981 aacagtggac gcgcgagctg gagatggtgg tcgaaaacgg ctgctatacc cttaaagaca
     2041 cttttgatga caccaccatt ctgggctgga tgatccagac ggaagatact gaatacagct
     2101 taccgcagcc ggaaattgca ggcgagctgc tgaaaatcag tggagcgcgt ctggaaaaca
     2161 aaggccagtt tgacggtaaa tggctggatg aaaaagatcc gctacaaaac gcctatgtgc
     2221 aggccaacgg tcaggtgatc aatcaggatc cgtatcatta ctacaccatt accgagagtg
     2281 ccgagcagga gctaattaaa gccaccaacg agctgcatct gatgtatctt cacgcaaccg
     2341 acaaggtgct gaaagatgac aacctgctgg cgctgttcga cattccgaaa atcctctggc
     2401 cacgtttgcg tctctcctgg cagcgtcgcc gtcaccatat gatcactggt cgtatggatt
     2461 tctgcatgga tgagcgtggc ctgaaggttt acgaatacaa cgccgactcc gcctcctgtc
     2521 ataccgaagc gggcttgatc ctcgaacgtt gggcggagca gggctataaa ggcaacggct
     2581 tcaatccggc ggaagggctg attaacgaac tggctggtgc ctggaaacac agtcgtgcac
     2641 gtccgtttgt ccatatcatg caggacaaag atatcgagga aaactatcac gcgcagttta
     2701 tggagcaggc gctgcaccag gcgggctttg aaacgcgtat cttgcgcggg ctggatgaac
     2761 tgggctggga tgctgccggg caactgattg atggggaagg gcgactggtt aactgcgtgt
     2821 ggaaaacctg ggcgtgggaa accgcgtttg atcagattcg tgaagttagc gaccgtgagt
     2881 ttgctgcggt gccaatccgt accggtcatc cgcaaaacga agtgcgtctt atcgacgtat
     2941 tgctgcgccc ggaagtgctg gtctttgagc cgctgtggac ggtgatcccc ggcaacaaag
     3001 cgattctgcc gatcctctgg tcgctgttcc cgcaccatcg ttacctgctg gataccgatt
     3061 tcactgttaa tgatgaattg gtgaaaacag gttacgcagt gaaaccgatc gccggtcgct
     3121 gtggtagcaa tatcgacctg gtcagccatc atgaagaggt gctggacaaa accagcggta
     3181 aatttgccga gcagaaaaac atctatcagc aactgtggtg tttgccgaaa gtggacggta
     3241 aatacattca ggtatgtacc ttcaccgttg gcggcaacta cggtgggacg tgtttgcgcg
     3301 gtgatgaatc actggtcatc aaaaaagaga gtgatattga accgttaatt gtggtgaaaa
     3361 agtaatacat gttgtagcaa ccccgtactg attatgaaga ataattagta cggggattaa
     3421 tcaaaatatt caatatttat atattccaat atttattcat ttcatatgtg aatatattaa
     3481 ccagtggaat acctgtgttt tgattatttc taaaggtttt gaatgaatgc ttattgtctg
     3541 atacacaaga aataacactc tttttatcgt taaaaaatga tatttcactt tgcccatgcc
     3601 gttaaagtca ctgataatgc gtccgttcgt aaattcaaaa tggcgtaatc taatatatgc
     3661 taaatttatt tgttggcctt gatatatata cggggctttt gttattactt gctctggcat
     3721 ttgtgttgtt ctacgaagca atcaatggtt ttcatgacac ggcgaatgcg gtggcaaccg
     3781 ttatttatac tcgtgccatg cagccacaac ttgctgtggt gatggcggca ttttttaact
     3841 tttttggcgt gttattgggc ggacttagcg ttgcctatgc cattgtccat atgttaccaa
     3901 tcgatttgtt gctgaatatg gggtcaactc acggtttggc gatggtcttt tccatgctgc
     3961 tggcggcgat tatctggaac ctgggaacgt ggttctttgg tttaccggcc tccagttcgc
     4021 acaccttgat tggtgcgatt atcggcatcg gtttaaccaa cgcgctgtta accggctcat
     4081 cggtgatgga tgcgttaaac ctgcgtgaag taaccaaaat tttctcctcg ctgattgttt
     4141 cccctatcgt cggcctggtc attgcgggtg gtctgatttt cctgctgcgc cgtttctgga
     4201 gtgggacgaa aaagcgtgac cgtattcacc gcattccgga agatcgcaaa aagaaaaagg
     4261 gcaaacgtaa accgccattc tggacgcgta ttgcgctgat tgtttccgct gcgggcgtgg
     4321 cgttttcgca cggcgcgaac gacgggcaga aagggatcgg cctggtaatg ctggtactgg
     4381 tggggattgc ccctgctggc ttcgtcgtca atatgaatgc gtccggctat gaaattaccc
     4441 gtacccgcga tgccgttacc aacttcgaac actacctgca acagcatcct gaactgccgc
     4501 agaagttgat tgcgatggaa cctccattgc ctgcagcatc gactgatggc acgcaagtaa
     4561 cagagtttca ctgtcatccg gcaaatacct ttgatgcgat tgcgcgcgtt aaaacgatgc
     4621 tgccaggcaa tatggaaagt tacgagccgt taagcgtgag tcagcgcagc cagctgcgcc
     4681 gcattatgct gtgcatctcc gatacctccg cgaagctggc gaaactgcca ggcgtcagta
     4741 aagaagacca gaacctgctg aaaaaactgc gcagcgatat gttaagcacc attgagtacg
     4801 ctccggtgtg gatcatcatg gcggtagcac tggcgctcgg cattggcacc atgattggct
     4861 ggcgtcgtgt agcgatgacc atcggtgaga agattggtaa gcgcggcatg acgtatgcgc
     4921 aaggcatggc ggcacaaatg acggcggcag tgtctatcgg ccttgccagt tatattggga
     4981 tgcctgtctc cacaacacac gtcctctcgt ctgcggtagc agggacgatg gtggtggacg
     5041 gcggtgggtt acagcgtaaa acggtaacca gcatcctgat ggcgtgggtg tttactttac
     5101 cggcggcaat ttttctttct ggtgggctgt actggatagc attgcagttg atttaacctt
     5161 cctgaaaatg cccggtcctg gcgatcgggc atttccattt ttgactagtg ataaccacgc
     5221 gcggtcataa aatccgtaat cgctttttct gcatcaacca acacctgttc cagtggctga
     5281 ttggcatcga tatcaaccag ttgtgcacca ccaaaggtta actgtggcgt tatggcaatc
     5341 ttcctcgcca gcgattcccg tttatggtcg ggtttacgtg cacaggcaac ttcaaggtca
     5401 acattgagtt tgatgaccag atcaggctta tggctcgcca tccagtgaaa cgctttacgt
     5461 tcctggcttg ccagccatga gacaaaacga ccaccttcaa cgttaggtgg gaacaccgta
     5521 ccatcgtaag cgccaggaat ttggtcctga ggataacggt cggttagaac aattaacccg
     5581 cgacgacgac aggcaagcat atgacgaaag cgcagtaagc gacgggcgac aaacgctgta
     5641 attaccagcg ccggaactgg tccaggcaat ttttttgctg ttttcacctg atttcgttca
     5701 attgttttat gtaaggattt tcccatcaaa ggtaattttg tcactgcacg accgacattt
     5761 ccggcctgtt ttcctaaatg aactctttcg gcagcaccat atttttcgac aacggtaata
     5821 agatgttcac acaccgttga cttgcctgaa ccatcgctac caataacggc aattaatgga
     5881 ggtgttattg actgcatgaa tatatccttt aatttaaata tccattaaaa atatttattt
     5941 ggttaatatg tttttatgaa agcgtaattc aggtcaatgt cacaattagc cattgtcaca
     6001 ataaggttga acggatattc tgagcacgga ccacgtaata ttcaatacat tatttactgc
     6061 cgggtttttc gtgaatcaac gtaatatgtc aataattaat tccacgccag tgcgtgttat
     6121 tgccattgta ggatgtgatg gttcaggtaa atcgaccctc acggcaagcc tggtaaatga
     6181 actggcagca agaatgccaa cagaacacat ttatctcggg caatcgtccg ggcgaattgg
     6241 cgaatggatt tcacagctcc ctgttattgg cgcaccttgt gggtgttatc tgcgaagtaa
     6301 agcggcacat gtacacgaaa agcccttaac tccgcctggc aatattactg cgctggttat
     6361 ctatctgctt tcctgctggc gggcgtacaa gtttcgcaaa atgttgtgta aaagccagca
     6421 aggctatctg ctcatcaccg accgctaccc gcaggttgaa gtgccgggtt ttcgctttga
     6481 tggcccgcaa ttggcaaaaa ccacgggcgg taacggttgg ataaaaatgt taaggcagcg
     6541 cgagttgaag ctgtaccaat ggatggcgtc ttatttaccc gtattgttga ttcgtcttgg
     6601 cattgatgaa caaaccgcgt ttgcgcgtaa acctgaccac caactggcag cgttacagga
     6661 aaaaatcgcc gttacgccgc aactgacatt caatggcgcc aagatccttg aactggatgg
     6721 tcggcaacca gccgatgaaa ttatgcaagc gtcactacgc gcaattcacg ccgcgctttc
     6781 ctgatcacca aaatggatag gggatgattt aacgaatgga tgcactacaa acacaaactg
     6841 ttaatagtac aaccgcgccg cagcccaact acattccggg gctgattgcg gtggtcgggt
     6901 gtgatggcac cggtaaatcc acgctgacca ccgacctggt gaaatcgctg caacaacact
     6961 ggcaaaccga gcgccgctat ctggggctgc tctccggcga agacggcgac aaaatcaaac
     7021 gattgccgtt ggttggcgtc tggctggaac ggcgattggc ggccaaatcc tcgaaaaccc
     7081 aaagcatgaa aaccaaatct ccggcgctat gggcggcggt gattatgtac tgcttctcgc
     7141 tgcgaagaat ggcgaatcta cgcaaggttc agcgactggc gcaaagtggc gttctggtgg
     7201 tcagcgatcg cttcccgcag gctgaaattt cgggctttta ttatgacgga ccggggatcg
     7261 gcgtcgaacg tgcgaccggg aaaatcagca tgtttctggc gcagcgcgaa cggagtttat
     7321 accaaaaaat ggcgcaatac cgtcctgaat taatcattcg tttggggatt gatattgata
     7381 ctgccatctc ccgcaagcct gaccatgagt atgccgagct gcaggacaaa atcggcgtca
     7441 tgtcgacgat tggctataac ggcacaaaaa ttcttgagat tgattccaga gcgccctaca
     7501 gcgaagtgct ggaacaggta cagaaggcgg tttctctggt tgccatcgtt tctgaccgcc
     7561 gaagtttaac ttaaggctgg cgggatattt tttactatca acgataaggt caggctgaat
     7621 tggcgggttt taacatcaaa cattggtttg cagatggcgc gtttcgcacc attattcgca
     7681 atagcgcctg gttaggctcc agtaatgtcg agagcgcctt gctggggctg ttggcgctct
     7741 cgtgtgccgg taaagggatg acgcccgcca tgtttggtgt actggtgatt gtgcaatcgt
     7801 acgccaagtc gatcagcgat tttattaagt ttcagacatg gcagctggtg gttcagtacg
     7861 gaacgccagc cttgaccaac aataatccgc agcaatttcg caatgtcgtc tcattttcct
     7921 tctcgctgga tatcgtcagc ggcgcggtgg cgattgtcgg cggcattgcc ttactgccat
     7981 tcctttccca ttcattaggt ctggatgacc aaagcttttg gctggcggcg ctgtattgca
     8041 cgctcattcc ttcaatggct tcctccacgc cgaccggcat tctgcgtgcg gtagatcgct
     8101 ttgatttgat tgctgtacag caggcgacga aaccttttct gcgcgcagcg gggagcgtcg
     8161 cagcctggta ttttgacttt ggttttgcgg gttttgttat tgcctggtac gtgtcgaatc
     8221 tggttggcgg caccatgtac tggtggtttg ccgcgcgcga attacgccgt agaaatatcc
     8281 ataacgcctt caaattgaat ctgtttgagt ctgcccgaca cattaaaggc gcgtggagtt
     8341 ttgtctggtc aaccaacatt gcccactcca tctggtcggc gcgtaactcg tgcagcacag
     8401 tgttggtggg gatcgtgtta ggacccgctg ccgccgggtt atttaaaatc gccatgacat
     8461 tcttcgacgc cgccggaacg ccagcgggtt tgctgggtaa aagtttttac ccagaggtga
     8521 tgcgtttaga tccgcgcacc accagaccgt ggttgttagg tgtgaagtcc ggtttactgg
     8581 cgggcggaat cggtattctg gtggctctcg cggtgttgat cgtcggcaag ccgctcattt
     8641 cgctggtgtt tggcgttaag tatctcgaag cgtatgacct gattcaggtg atgctgggcg
     8701 caattgtgat ttcgatgctg ggcttcccgc aggagtcatt gctattgatg gcgggaaaac
     8761 agcgcgcttt tctcgtggcg caaaccatcg cctcaatcgg ctacatcgta ttgctgttca
     8821 tgttctgtca tctgtttggt gtgctgggag cagcgtttgc ttacttcggt gggcaatgtc
     8881 tggacgtggc gctctcgtta attccaacct taaaggcatt ttttcagcgc cattccttgc
     8941 tttataacgc agccggagag aaatcctgat gatgagaaaa tatttcccgc ttgaggcgag
     9001 cgagcgtcta tttgttgcga ttgaagaaga tgatgtcgtt gatgcacagg ttagtttgcc
     9061 gccgactatt gcactaagtt gtaccaccga aatcattcat gataattacg cgttatgcct
     9121 gcaattctgg ctcaatggcg ttgatcgtca ggaattactg cgtttagttc gcaaacaagc
     9181 caaaggcgat gagctgactg ctgatgagcg caaacaattt aaatacatgc gagcgcgcta
     9241 caagcacctg cgttttgcgc aacggctgta tttaaagaag catcaggccg gatttttatt
     9301 cggcaaaacg accgtttttc tggggcgttt tcaggacggc tttcgtaacg gtaagaaaaa
     9361 tattgtgtcg tattacggca acctgttacg gatttattta agctctccgg tctggtcgtt
     9421 ggtgaactat tcattacgcc atagccagtt agaaagcgta agcagtttta ttgcctatcg
     9481 gcaaaagcaa atgcataccc tgaaagagat tattgccaaa ccgcgactaa ccggcaggga
     9541 atttcatgat gtgcgaaaaa tcatcagcca gcaggtttct tattacgaca ccttaagatc
     9601 gcttgatcct gaaaataaag aggcgttaca gatttcccgt ttcctcgccg ccattaatgg
     9661 cctgatggga gataaacacg acgacatggt ggcggatgat atggaaaacc gccagtcgta
     9721 ttatgcaccg ttggcgctgg atagcgatat ttgtcagcgc ttagagctgc tgatttcgcg
     9781 ttttccgcta tagccggagc aatgatgtca tctcgtttga ttattgcgtt gattatcatg
     9841 ctcttagccc cgggtgtgca ggcacacaat ttcgtcactg gcaaaacggt tacccctgtt
     9901 tatatccagg aaggcggcga gctgcttctg aatagcgacg acgaaattca ctaccaaaag
     9961 tggaacagca cacaattagc gggaaaagtc cgcattattc agtatatcgc cgggcgtaag
    10021 tcggcgaaga aaaagaactc actgttaatt aaagcggttg aagcggcaaa ctttccgcag
    10081 gatcgttttc agcccaccac cattgtgaac accgatgatg ccatctttgg tactggctat
    10141 tttgttgttg gcaaaatcga aaaaaataaa cgccgctatc cgtgggcgca attcgttatt
    10201 gacggcaatg gacagggacg cgttgcctgg cgcttgccgg agcagagttc aaccattctg
    10261 gtgctgaata aagccgggca aattcagtgg gcaaaggacg gcagcctgac gccggaagag
    10321 gtagatcatg tcattgcgtt ggcgcagaaa ctgatcaatg aataaaaaac tcaaaggatt
    10381 gagggcgtta gtgagatgaa gctggttgaa cactacatca tgcgtggtac gcgccgtctg
    10441 gtgctgatta tcgtcggttt tctgatcttt attttcgcca gctactccgc acagcgttat
    10501 ctgaccgaag cggcaaatgg gacattagct cttgatgttg tgctggatat cgttttctac
    10561 aaagtactga ttgcactgga gatgttgtta cctgttggtc tgtatgtgtc agttggcgtg
    10621 acgctagggc agatgtatac cgactcggaa attaccgcta tctctgcggc ggggggcagt
    10681 ccgggacgct tgtacaaagc cgttctttat ctggcgatac cgctaagtat ttttgtcacc
    10741 cttctgtcga tgtatggtcg tccgtgggct tatgcgcaga tttatcaact ggagcagcag
    10801 tcgcagtcgg agctggatgt tcgccagttg cgggcaaaga aatttaacac taacgataac
    10861 ggacgaatga tcctttcgca gacggttgat caggataata atcgcctgac tgacgcgctg
    10921 atttatactt ctactgccaa tcgaacccgt attttccgcg ccggttcggt tgatgtggtt
    10981 gacccatcac ccgagaaacc gaccgttatg ttgcacaacg gcactgccta tcttctcgat
    11041 catcaggggc gtgacgacaa cgaacagatc taccgtaatc tgcaattaca tctgaatccg
    11101 ctggatcaaa gcccgaacgt caaacgcaaa gcgaaatcgg tcacggagct ggcgcgctcc
    11161 gcctttcctg ccgatcatgc cgaactgcaa tggcgacaaa gccgtggcct gaccgcattg
    11221 ttgatggcgc tgctggccat ttcattaagt cgggtaaaac cgcggcaagg gcgattttca
    11281 acgttattgc cactgacgtt gctgtttatt gccatttttt atggcggcga cgtctgccgt
    11341 acactggtgg ctaacggcgc gattcccctc attcctggtt tgtggttagt gcccggactc
    11401 atgctaatgg gcctgctgat actggtcgca cgtgacttct ctttgctgca gaaattttcc
    11461 cgatgaatgt tttcagtcgc tatttaatcc gtcatctctt tctcggtttt gccgccgctg
    11521 cagggctatt gctgccgctt tttaccacct tcaacctgat taacgaactg gatggtgtca
    11581 gcccgggcgg ttatcgctgg actcaggcgg tgctggtggt gctaatgacc ttaccgcgca
    11641 cactggtcga actttcgccg tttatcgcgt tattgggtgg tattgttgga ctggggcagt
    11701 tatcgaaaaa cagtgagctt accgccattc gcagcacggg gttttctatc ttccgtattg
    11761 cactggtggc gctggttgcc gggatattgt ggactgtttc gttaggcgcg atagatgagt
    11821 gggtggcgtc gccattgcag caacaggcgt tgcaaatcaa atcgaccgcc accgcgttgg
    11881 gggaggacga tgacattacc ggcaatatgc tgtgggccag gcgtggtaat gaatttgtga
    11941 cggtgaaatc gctgaacgag cagggccagc cggtgggcgt ggagatattt cattatcgcg
    12001 acgatctctc gcttgaatcc tacatttatg cacgcagtgc caccattgaa gacgacaaaa
    12061 cgtgggtcct gcatggtgtg aatcataaaa aatggctgaa tggcaaagaa acgctggaaa
    12121 cattagataa tcttgcctgg caatcggcct tcaccagtat gaatcttgaa gagttatcaa
    12181 tgccggggaa tactttttct gtccgtcagc ttaatcatta catccattat ttgcaggaaa
    12241 ccgggcaacc cagcagcgaa taccgccttg cactgtggga aaaactgggg caacccatcc
    12301 tgaccctggc gatgattttg ctggctgtgc cgtttacctt tagtgccccg cgttcgcctg
    12361 ggatgggtag ccgtctcgct gtaggcgtca tcgtgggctt actcacctgg atcagctatc
    12421 aaatcatggt taatctggga ttgctatttg cgttaagcgc acctgttacc gcgctcggtt
    12481 taccggtagc gtttgtgttg gtggcgttga gcctggtgta ttggtatgac agacaacatt
    12541 aaacccgccg tggcgcaaac accacggcgg gctcacatta ctgcttcaga gtagcaaatg
    12601 cctgaatgat ggcatcgatt tgctcacgac tgtgggcagc atttacgcta cagcgcagca
    12661 gcgtgatgcc tgcaggcgcg gcaggcggca ggataagatt cacatacacg cccggcgaga
    12721 taagctcgcg ccagatgcgt aaaccttcct ctttcgaacc gattattact ggcactaccg
    12781 ggctaatatg tgaacttaac tcataaccca gtttttgcag accgtcatac agatggttgg
    12841 cgttatccat cagcttttgc cgcagctctg gttgtgtggc aatagttctt aacgacgagc
    12901 gcacggtcgc aatgcaggac ggagaaggag aggcggtaaa aatatacggg cggctgccgt
    12961 agcgcagcac ttccatcgct tccgaaccga cagcaaaacc gccaatcgag gccagacttt
    13021 tactgaaagt accaacgata atatccacat catcttcaac gccgacagct tcagccagtc
    13081 cgcgccccgt tgcgcctaac acgccaaacg aatgcgcttc atcgacaatc aaataaccac
    13141 cgagacgacg tttgatatca acaatttccg ccagcggcgc aacgtcaccg agcatgctgt
    13201 agatgccctc aacgatgata atcgcctctt tcgcccgttc accgaggcga accatgcgcc
    13261 gttccagatc tttagcatcg ttatggcgga aacgaataat ttccgcgcca ccgagagaac
    13321 aggcgtcata aatactggcg tggctgtcgg catccagtaa caccacggcg ttatggtcgg
    13381 caagtgcgct gatgacgcct aaattagcgg tataaccggt tgaaaaacga tagcggtagg
    13441 gagattgaaa aactcggcaa tctctttctc taacgccagg tgcggcgcat aactgccgtt
    13501 tgccatacga gaaccggtgg tgccggtgcc ttgggccgct aacgcggcct gaccttcgct
    13561 gatggcatca tgattaaagg ttaaaccgag ataattattg gtcccggcca gaatcacttt
    13621 tttattgccg atccggcctt cggtggcgga atacacttca tcaatacagg tgccaaacgg
    13681 tgttaacccg ttgtcactaa attgcagacg ctcgccagca aggcgcgcat atttatcgta
    13741 tagccccatt atttattctc cagccatgga agtagagttt ccattaattg cgcaggggtt
    13801 ttgacatcca gcagaatatt aataggaatg gaaatatcga atctgtcttc cagcatcatt
    13861 aagagatcca tcactttgat cgactccagg ccaagatcgt tgacgagatc gctgtcgggc
    13921 ttaatttcca caccgttttc gaccaaatcc tgtaaacagg aaaggatgta atccattact
    13981 atttcacgat ttaccataga aacgttaaca cacctttaaa acaagacaat caaaatagcc
    14041 cgttgcgtaa tgcgtgttcc aggctaatcc cgggaaacca attgatatct tcagaaatac
    14101 ggttattact tgccgaccag tcggcgtggg ttaattcacg aattttcgag cgggtcagca
    14161 taggttcttt acctgccagg cgactcaacg cggtactgat atccgcaagg cctgtgagta
    14221 ccggcagagg aatgtcaacc attcgcacgg aaccacaacg ggctttggcg gcaagctgtc
    14281 gtacgcgttg ccagtcatag ccaccggcga cgccatcgca taattcatag gtttgcgtct
    14341 gtacagtttc ggcgcttaac cactgaccca ctgcttgcgc aaaatcggtg acgtgaagaa
    14401 aagagagttg tgtatctggc gcaccaagtc gtggcagcag gccgcgtagc atccagtcaa
    14461 acagcggttt taactcttta tcgccgggac catacacggc tgtcgggcga aaaacgccca
    14521 gcgtaatttc gtccgccatt gctgccagtc gttgctcggc gacgtatttg gattttgcat
    14581 accaggagag ctcgggatgg cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa
    14641 agccgctctc ttttgctgct tgcatcaggc gcaggctgcc gtcaacgtta cagcgggtga
    14701 aaatctcttc tttgtgcccg cgcacttgcc cggcgcaatg gaccactacg ctggctccgg
    14761 caaccagctc gctaagtgaa tgagtatctt ccagcgaacc gcgcacccag gtgagattat
    14821 cattgacgtg agcgcgggca gtacgcgtca atgcgcgaac gtgaaagccg cgggcgagca
    14881 ggttatcgat aatatattta ccgataaacc cggtagcgcc cgtcaccgcg acagtttgat
    14941 ttatgccagg gattcctgca cattaagact ggcagcataa gccttctgat aacgtttttt
    15001 cgcttccgca cgggcaggct tgccggagga cgttcgggga atactgtggg gcggcaacag
    15061 atcgatagcc gcggtcacgc caaattcgct ttggatccgt gccgccagcg cgtggataag
    15121 ctgcccgcga cgttcttcgt cgctgatccg acactggatc tgcaaaatga ttttttcctg
    15181 ggcggtaaca aaagcaatcg catcgccaga atgaatttcc ggttcctgtt ccgctatata
    15241 ttcaatatcc tgcggccaga tattacggcc acgaataata atcagatctt taatgcgtcc
    15301 ggtgacatac agataaccgt ccagcagata accgaggtcg ccggtgtcta accagcccgt
    15361 cgcggcaatc tcgtcttgcg aaacctggtc gccaaagtaa ccgctcatca gactgggacc
    15421 ggagatgcaa atatggccta ccacacgttc cgcgaccggc atacctgctt cattgcggat
    15481 ttcaatacca tgttccggca acgctttgcc gcagttgacg aaagtcgata cggcgcgtgt
    15541 ctctgcaccc ggcgcgacgg ctttaccctg atattcgagg atgtcgcgat ccacttcgtt
    15601 aaccacaacc ccggaggctt catcagagaa gctgacagcc agcgcatttt ctgccagtcc
    15661 gtagcacggc atgaaagttt tattgtcaaa gttaacctga cggaaacatt cagcgaattg
    15721 atggagttgt tctgcggaga tcggctcagc accaatacca gcgacgcgcc agcaggaaag
    15781 atccagttca gcgagatctt tttcattcac gcggcgttgg cacaattcat agccaaacgg
    15841 cggcgcaacg gaaacggtgc cgcgattttt actgatcaat ttaagccatt gcaaaggacg
    15901 catggcaaaa tcctgagtgc gcaaataatc tactgaaagt tgcgtggcga cggggggcag
    15961 gagaaagccg accagtccca tatcatggta gaaaggctgc caggagacgc agcggtcgct
    16021 agggcgtaat ttaatgccat cgtggcttat agcacgtaga ttagccatca cttcgcgatg
    16081 ggtgataatg acgccacggg gaaaacgggt gctgccggag gtgtactgga ggtaggcgat
    16141 atcgttcgga actggacgct ggagcgcaac atcggcttcc ggtaatgcct taaaccaagc
    16201 gtggcttaaa acatgtaatt cggggttgtt atgcgtcgcg gcattgacca gtggcaacca
    16261 ctcatcacca gtgataatgg ctgcgggctg gcaacttgcc agtaaaccct ggagtttggc
    16321 gctccaggaa tcccgctgac cgacgcccat tggaatcgcc aacgggacgg cgactaagcc
    16381 agcatactga caggcaaaaa aagcctctac aaacccgcta ctcgtttcgg caatcagcgc
    16441 cacgcgttcg ccttttttca ggttcagcga taacaaccgc ttcgcaccag cttcggcacg
    16501 cgtttttaat gtctgatatt ccagttgatc ttcaagttgg caacgtctgt cataaaaatt
    16561 cattccggct ctactcagag cggcgtagtc caaagcatca accagcgttg gaaaatcggc
    16621 atagcgcata ggtagggaat gcgtaaagat tttattagac atatacacca cgaaaaaatt
    16681 ggatatcaaa aataaaatga caataatgcg gtggattata taggcataaa agataaatga
    16741 gatagtattt tcatcggtgt gaaaatgaac attgatttaa ataatgagtt taagtttttt
    16801 gtttttatta tgaatgatat ttaatttatg aatatattaa gaagaagcct tgtccggtcg
    16861 ccttaaattg acaaaaggca ttgaggcgtt tctgtgtagc ctctgacctt atcataatca
    16921 ttaattaata ctatttattc gtcgtttgta ttcattgagt tgattattaa gatgccgctc
    16981 gttgctgttg caacctgttt ttcaccatta attatttgtt atccacagag atatggaatg
    17041 cgatctgctg atgattaaaa ttgaaaaagt aattaataaa aatgacctta aggcatttat
    17101 cgcttttccg tcatcacttt atcccgacga tccaaactgg ataccccctt tattcattga
    17161 acgcaacgag catttgtctg cgaaaaatcc ggggacggac catatcatct ggcaggcgtg
    17221 ggtggcaaaa aaagcggggc agatagttgg gcgtattacc gcgcaaatag ataccttgca
    17281 ccgtgagcgt tacggtgaag ataccggtca tttcggcatg attgacgcca ttgatgatcc
    17341 gcaggatttt gccgcgttgt ttggcgcagc ggaagcgtgg ttgaagtcac aaggtgcaag
    17401 taagatcagc gggcctttca gcctgaatat caatcaggaa agcggattac tgattgaagg
    17461 ttttgacaca ccaccctgtg cgatgatgcc acacggcaaa ccgtggtatg ccgcgcatat
    17521 tgaacaactg ggttatcaca aaggcattga tttactggcg tggtggatgc agcgaaccga
    17581 tctcactttc tctccggcgc taaaaaaact gatggatcag gtgcgcaaaa aggtgaccat
    17641 tcgctgcatc aatcgtcagc ggtttgccga agagatgcag atcctgcgtg agattttcaa
    17701 ctctggctgg cagcacaact ggggatttgt gccgtttacc gaacatgaat tcgcgaccat
    17761 gggcgaccaa cttaaatatc tggtgccgga cgatatgatc tacatcgctg agattgattc
    17821 tgcaccctgc gcgtttattg tggcttgcct aacatcaacg aggcgattgc cgatctgaac
    17881 ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg
    17941 cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc
    18001 ccggtgattg cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc
    18061 gatgcgctgg agatgtcatg gatcctcgaa accaatacag gcatgaataa catgctggaa
    18121 cgcattgggg cggagccgta caagcgttac cgattgtatg aaaagcaaat ttaataccag
    18181 atcctgttta gcgcaggggc ggtcattcgg gagagggtat agatgaacag atttaacacc
    18241 agatcctgtt aagtgcaggg gcggtcccct cgccttgggg atagggtaaa actctgctca
    18301 cattcccacc ttgttcatct ccagcggcac cgccgagcga atcaaatggt ggccttccag
    18361 ctcgatatcg tggagattct ttttcacggt ccgtacatga tcgcgcgctg cgcgctgagc
    18421 gacgtgcggc agccgctgca acaccgcgtt gtagatccgc gcatgctggc gatcgatctg
    18481 ctttttttgc tgtggtcgat ggtagagatt atttacgctg gcaaacactg aattaaacat
    18541 cagatcggtc aatgattgca gcgtaaacac cagcacctga ttgtgagaag cctgacaaat
    18601 ggcgagatgg aaagcgtgat ccaactgcgc atgttcgatc agcgaaatct ctttgtggtt
    18661 ctcactggcg gcgagcattt tttcataaca gcgggttatc acaacaaaat cagcctgcgt
    18721 tcccagcgtt gccgccagcc ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag
    18781 atcgtacagc gttcgcggct gcgtactgaa cagatggatc agcgggctgg tgtcctgctc
    18841 ccgattaagc cgtgcgacat gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc
    18901 gcgcagcacg gtcagccctt cacgcagtgc ggagcgtgag aagccgagct tttcacacag
    18961 tcgacgttcc gagggaagcg gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc
    19021 gatactctct gcaaccactt cgcaaatagg gcgacgttca tctttcattc ccggacctcg
    19081 tgcacaggta ggaccaattt ttctgttctt aactcgcaaa acacgcacat cacgcaagtg
    19141 attatgttac atcaatttaa cattaagtta accaagacaa ggtcacagag ctggaaaaaa
    19201 aatggtctga ccggtagcta aagagataga cgaaaacgaa aagcccgctt aataactgtt
    19261 cacagaagca gcgcgcaaaa atcagctgcc acacaacaca acaaagcgaa gcctactcat
    19321 gagcatcttg tacgaagagc gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt
    19381 actgatggca ctgcgtgagc atgtccctgg acttgagatc ctgcataccg atgaggagat
    19441 cattccttac gagtgtgacg ggttgagcgc gtatcgcacg cgtccattac tggttgttct
    19501 gcctaagcaa atggaacagg tgacagcgat tctggctgtc tgccatcgcc tgcgtgtacc
    19561 ggtggtgacc cgtggtgcag gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg
    19621 tgtgttgttg gtgatggcgc gctttaaaga gagcctcgac attaaccccg ttggtcgccg
    19681 cgcgcgcgtg cagccgggcg tatgtaacct ggcgatctcc taggccgttg cgccgcataa
    19741 tctctattac gcgccggatc cttcctcaca aatcgcctgt tcaattggcg gcaatgtggc
    19801 ggaaaatgcc ggtggcgtcc actgcctgaa atatggtctg accgtacata acctgctgaa
    19861 aattgaagtg caaacgctgg acggcgaggc gctgacgctg ggatcggacg cgctggattc
    19921 acctggtttt gacctgctgg cgctgttcac cggatcggaa ggtatgctcg gcgtgaccac
    19981 cgaagtgacg gtaaaactgc tgccgaagcc gcccgtggcg cgggttctgt tagccagctt
    20041 tgactcggta gaaaaagccg gacttgcggt tggtgacatc atcgccaatg gcattatccc
    20101 cggcgggctg gagatgatgg ataacctgtc gatccgcgcg gcggaagatt ttattcatgc
    20161 cggttatccc gtcgacgccg aagcgatttt gttatgcgaa ctggacggcg tggagtctga
    20221 cgtacaggaa gactgcgagc gggttaacta catcttgttg aaagcgggcg cgactgacgt
    20281 ccgtttggca caggacgaag cagagcgcgt acgtttctgg gccggtcgca aaaatgcgtt
    20341 cccggcggta ggacgtatct ccccggatta ctactgcatg gatggcacca tcccgcgtcg
    20401 cgccctgcct ggcgtactgg aaggcattgc ccgtttatcg cagcaatatg atttacgtgt
    20461 tgccaacgtc tttcatgccg gagatggcaa catgcacccg ttaatccttt tcgatgccaa
    20521 cgaacctggt gaatttgccc gcgcggaaga gctgggcggg aagatcctcg aactctgcgt
    20581 tgaagttggc ggcagcatca gtggggaaca tggcatcggg cgagaaaaaa tcaatcaaat
    20641 gtgcgcccag ttcaacagcg atgaaatcac gaccttccat gcggtcaagg cggcgtttga
    20701 ccccgatggt ttgctgaacc ctgggaaaaa cattcccacg ctacaccgct gtgctgaatt
    20761 tggtgccatg catgtgcatc acggtcattt accttttcct gaactggagc gcttttgatg
    20821 ctacgcgagt gtgattacag tcaggcgctg ctggagcagg tgaatcaggc aattagcgat
    20881 aaaacgccgc tggtgattca gggcagcaat agcaaagcct ttttaggtcg cccagtcacc
    20941 gggcaaacgc tggatgtgcg ttgtcatcgc ggcattgtta attacgaccc gaccgagctg
    21001 gtgataaccg ctcgtgccgg aacgccgctg gtggcgattg aagcggcgct ggagagcgcc
    21061 gggcaaatgc ttccctgcga gccgccgcat tatggtgaag aagccacctg gggcgggatg
    21121 gtcgcctgcg ggctggcggg gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc
    21181 ctcggcacgc gcatcattac cggcactgga aaacatctgc gttttggtgg cgaagtgatg
    21241 aaaaacgttg ccggatacga tctctcacgg ttaatggccg gaagctatgg ttgtcttggc
    21301 gtgctcactg aaatctcaat gaaagtgtta ccgcgaccgc gtgcctctct gagcctgcgt
    21361 cgggaaatca gcctgcaaga agccatgagt gaaatcgccg agtggcaact ccagccatta
    21421 cccattagtg gcttatgtta cttcgacaat gcgttgtgga tccgccttga gggcggcgaa
    21481 ggatcggtaa aagcagcgcg tgaactgctg ggtggcgaag aggttgcggg tcagttctgg
    21541 cagcaattgc gtgaacaaca actgccgttc ttctcgttac caggtacctt atggcgcatt
    21601 tcattaccca gcgatgcgcc gatgatggat ttacccggcg agcaactgat cgactggggc
    21661 ggggcgttac gctggctgaa atcgacagcc gatgacaatc aaatccatcg catcgcccgc
    21721 aacgctggcg gtcatgcgac ccgctttagt gccggagatg gtggctttgc cccgctatcg
    21781 gctcctttat tccgctatca ccagcagctt aaacagcagc tcgacccttg cggcgtgttt
    21841 aaccccggtc gcatgtacgc ggaactttga ggagcaggct atgcaaaccc gattaaccga
    21901 agagatgcgg cagaacgcgc gctggaagcc gacagcatcc tgtgcgcctg tgttcactgc
    21961 ggattttgta ccgcaacctg cccaacctat cagcttctgg gcgatgaact ggacgggccg
    22021 cgcgggcgca tctatctgat taaacaggtg ctggaaggca acgaagtcac gcttaaaaca
    22081 caggagcatc tcgatcgctg cctcacttgc cgcaattgtg aaaccacctg tccttctggt
    22141 gtgcgctatc acaatttgct ggatatcggg cgtgatattg tcgagcagaa agtgaaacgc
    22201 ccactgccgg agcgaatgct gcgcgaagga ttgcgccagg tagtgccgcg tccggcggtc
    22261 ttccgtgcgc tgacgcaggt agggctggtg ctgcgaccgt ttttaccgga acaggtcaga
    22321 gcaaaactgc ctgctgaaac ggtgaaagct aaaccgcgtc cgccgctgcg ccataagcgt
    22381 cgggttttaa tgctggaagg ctgcgcccag cctatgcttt cgcccaacac caacgcggca
    22441 actgcgcgag tgctggatcg tctggggatc agcgtcatgt cagctaacga agcaggctgt
    22501 tgtggcgcgg tggactatca tcttaatgcg caggagaaag ggttggcacg ggcgcgcaat
    22561 aatattgatg cctggtggcc cgcgattgaa gcaggtgccg aggcaatttt gcaaaccgcc
    22621 agcggttgcg gcgcgttcgt caaagagtat gggcagatgc tgaaaaacga tgcgttatat
    22681 gccgataaag cgcgtcaggt cagtgaactg gcggtcgatt tagtcgaact tctgcgcgag
    22741 gaaccgctgg aaaaactggc aattcgcggc gataaaaaac tggccttcca ctgcccgtgt
    22801 accctacaac atgcgcaaaa gctgaacggc gaagtggaaa aagtgttact tcgtcttgga
    22861 tttaccttaa cggacgttcc cgacagccat ctgtgctgcg gttcagcggg aacatatgcg
    22921 ttaacgcatc ccgatctggc acgccagctg cgggataaca aaatgaatgc gctggaaagc
    22981 ggcaaaccgg aaatgatcgt caccgccaac attggttgcc agacgcatct ggcgagcgcc
    23041 ggtcgtacct ctgtgcgtca ctggattgaa attgtagaac aagcccttga aaaggaataa
    23101 aaaaatgaaa actaaagtca ttcttagcca gcaaatggcg agtgcaatta ttgccgcagg
    23161 tcaggaagag gcgcagaaaa ataactggtc tgtttccatt gctgttgccg atgacggcgg
    23221 tcatctgctg gcgttaagtc gcatggacga ttgcgcgccg attgcggctt acatctccca
    23281 ggagaaagcg cgtaccgccg cgctggggcg tcgtgaaact aagggctatg aagagatggt
    23341 gaacaacgga cgtaccgcgt tcgtgactgc gccgttactg acgtcgctgg aaggcggcgt
    23401 accggttgtt gttgatgggc aaattattgg tgccgtgggc gtttctggtt taaccggagc
    23461 acaggatgcg caggtcgcga aagtggcagc agcggtgttg gcgaaataag cgaaaacgag
    23521 gagagaaaca atgagtcaaa ccataaccca gggccgttta cgcattgacg ccaattttaa
    23581 acgttttgtg gatggagaag ttttgccggg cgtggaactg gacgctgctg cattctggca
    23641 caatgttgat gagatcgttc acgatctggc accagaaaat cgtcagttgc tggcagaacg
    23701 cgatcgcatt caggcggcgc ttgatgagtg gcatcgcagc aatccggggc cggtaaaaga
    23761 taaagcggcc tataaatctt tccttcgtga actgggctac ctggtgccgc aaccggagcg
    23821 cgtgacggtg gaaaccacgg gcattgacag cgaaatcacc agtcaggcgg ggccacagct
    23881 ggtggttccg gcaatgaacg cccgctacgc gctgaacgcg gcgaacgctc gctggggctc
    23941 actgtacgat gcgttatacg gcagcgacat catcccgcag gaaggggcga tggtcagtgg
    24001 ctacgatccg cagcgcggtg agcaggttat cgcctgggtt cgacgtttcc tcgatgaatc
    24061 actaccgctg gaaaacggca gctatcagga tgtggtggcg tttaaggtgg tcgataaaca
    24121 attacgcatc cagttgaaaa atggtaaaga aaccacgtta cgtactccag cacagtttgt
    24181 cggttaccgt ggcgatgccg ctgcgccgac ctgcattttg ctgaaaaata acggcctgca
    24241 tattgaactg caaattgatg ccaacgggcg gattggcaaa gacgatccgg cgcacatcaa
    24301 cgatgttatc gtcgaagctg ctatcagtac cattctcgac tgcgaagatt cggtcgcggc
    24361 ggttgatgcg gaagataaaa tcctgctgta ccgcaatctg ctgggcctga tgcaggggac
    24421 tctgcaagag aaaatggaga aaaacggtcg gcaaatcgta cgtaaactga atgacgatcg
    24481 tcagtacacc gccgccgatg gctctgaaat ttctctgcac ggacgctcgt tgctgtttat
    24541 ccgcaacgtg ggtcatttga tgaccattcc tgtgatttgg gacagcgaag gcaatgaaat
    24601 cccggaaggt attcttgatg gcgtcatgac tggcgcgatt gccctctatg atttaaaagt
    24661 gcagaaaaac tcgcgcactg gcagcgtcta tattgtgaaa ccgaaaatgc acggtccgca
    24721 ggaagtggcg ttcgccaaca aactgtttac ccgcattgag acaatgctcg gtatggcacc
    24781 gaataccctg aaaatgggca ttatggatga agaacgccgg acctcgctga acttgcgtag
    24841 ctgtatcgct caggcgcgca accgcgtggc gttcatcaat accggtttcc tcgaccgtac
    24901 cggcgatgaa atgcattcgg tgatggaagc tggcccgatg ctgcgtaaaa accagatgaa
    24961 atcgacgccg tggatcaaag cctacgaacg taataacgtg ctttccggtc tgttctgtgg
    25021 gctgcgcggt aaagcgcaaa tcggtaaagg catgtgggca atgccggacc tgatggcaga
    25081 catgtacagc cagaagggcg accaactgcg tgccggggca aacacagcct gggttccgtc
    25141 accaaccgct gctacgctcc atgcgctgca ctaccaccat accaacgtac agagcgtgca
    25201 agccaacatt gcccagaccg agttcagtgc tgaatttgaa ccgctgctgg acgatctgct
    25261 gactattccg gtggctgaaa acgctaactg gtcggcgcaa gagatccaac aagagctgga
    25321 taacaacgtg caggggattc tggggtacgt ggtgcgttgg gtggagcagg ggattggttg
    25381 ttcaaaagtg ccggatattc acaacgtggc gctgatggaa gaccgcgcaa cgctgcgtat
    25441 ctccagccag catatcgcca actggttacg tcacggtatt ctgaccaaag aacaggtgca
    25501 ggcgtcgctg gagaatatgg cgaaagtggt tgatcagcaa aatgctggcg atccggctta
    25561 tcgtccgatg gcggggaatt tcgctaactc gtgtgctttt aaagctgcca gcgatttaat
    25621 cttcctcggc gtgaaacagc caaacggtta taccgaaccg ttattacacg cctggcgttt
    25681 acgcgaaaaa gaaagtcatt aatagcgtca aaaaagcccc ggcaagtttt attgtcgggg
    25741 ctatgatagt aactgcggta gtacatttat cccggaaata accggagcga ttatcagggt
    25801 ttaacaatat taaatacccg tagcgcatta ctagcgggcc taaaacaaca atatggacat
    25861 cttaacgata ctctgcgcct gcgtgtgcgg aacagaaacc cttacccgat atacttgtta
    25921 tgccagaggt agcaattgcg ttagcggcgt actgttgctg taaagtataa cgcctatatt
    25981 ttacagttca gtcctctttt gcttattaca accttaagta tatcaagcat ataaagataa
    26041 taagagactg aacaatatgg ttacctggac ccaaatgtat atgccgatgg gaggactggg
    26101 gctatccgct ctggtcgccc tgatcccgat aatattcttc ttcgttgcac tcgcggtatt
    26161 acgtctgaaa ggacatgtcg ctggagcaat aacccttata ttatctatcc tgattgcgat
    26221 attcgccttt aaaatgccga ttgatatggc atttgctgct gcgggctatg gctttattta
    26281 tggattatgg ccaattgcgt ggattattgt cgcggcggtg ttcctgtata aattaaccgt
    26341 tgccagcggg cagttcgata ttatccgcag cttggttatc tccatcaccg acgatcagcg
    26401 tttgcaggtg ttactgattg gtttctcctt tggtgcgttg ctggaaggag ctgctggctt
    26461 tggtgcgacg gtggcgtttg gtgcgttggg cgtgccgatt ctggtcgccg gtcaggtaac
    26521 gggaatcgat ccgttccaca ttggcgcaat ggcgggacgt cagttaccgt tcctgtcggt
    26581 tcttgtgccg ttctggctgg tagcaatgat ggacggctgg aaaggggtga aagagacgtg
    26641 gccagcggcg ctggttgctg ggggaagctt cgctgtcact cagttcttta cctctaacta
    26701 tattggtccg gaactgccgg atattacttc ggcgctggtg agtatcgtct cactcgcttt
    26761 atttcttaaa gtctggcggc cgaaaaatac cgaaacggca atcagcatgg ggcaatccgc
    26821 aggtgcgatg gtggtaaata agccatcttc tggcggtccc gtgccttcag aatatagtct
    26881 ggggcaaatc attcgtgcgt ggtcaccgtt tttaatctta acggtgctgg tcaccatctg
    26941 gaccatgaag ccgtttaaag cgttatttgc tccgggcggc gcgttttatt cactggtgat
    27001 taatttccag atccctcatt tgcatcaaca agtgttgaaa gcggcaccca ttgtcgccca
    27061 accaacgcca atggatgcgg tgtttaaatt cgaccctctc tcggctggcg gcaccgctat
    27121 ttttattgcg gcgattatct ctatcttcat cctcggcgtg ggcatcaaga aagggattgg
    27181 cgtctttgcc gaaatgctaa ttagtctgaa gtggccgata ttgtcgattg gcatggtgct
    27241 ggcattcgcc ttcgtcacca actattccgg catgtccacc acgctggcgc tggtactggc
    27301 aggtacaggc gtgatgttcc cgtttttctc accgtttctc ggctggctgg gcgtattcct
    27361 taccgggtcg gacacttctt caaacgccct gtttggttca ctgcaatcga ccacggcgca
    27421 gcaaatcaac gtctctgaca ctctgctggt ggcagcaaat accagcggcg gcgtaactgg
    27481 caagatgatc tccccgcaat ctatcgccgt ggcctgcgcc gcgacgggca tggtgggccg
    27541 agaatctgaa ctgttccgct acaccgtgaa gcacagtctg atttttgcca gcgttatcgg
    27601 cgttatcacc ctgctgcagg cgtatgtgtt taccgggatg ttagtctcgt aaatcaataa
    27661 agccggatga aggtaaatat cacccggctt tccatttgtc tgtcgtttat ctcgccaggg
    27721 aaccgttttt acgattggct tctttatacg tagtctccgt tttacgatgc gaattgttta
    27781 atatttatac aacttggctt ctgtgctggt tataaagtac ccactttcat gagatcaatt
    27841 ttagtatgtt attacattgc ttcatctgtt gctttcatga cgaatggcgt tttttctctt
    27901 atttatattt ttcactagaa caatgagtag atcaaaattc ccaaaactcc caaaaacata
    27961 agattattcg caaagctgat tttgcagaat aatgagaact tttaagattt gtcgaggatc
    28021 aattttaagc aatttatctc cacttgttac ataaaagatg ccgttaggtt aataagttaa
    28081 tcttgctata acagtgttcg ttactgccta ttaataaaga tatttgggat agggtaacgt
    28141 ggatgaggat tcgaaatcat ccgtcttgcg gaaagtgaaa tattggcaga tgttaatatt
    28201 atgtatgtat tttaactcca acaacacacc aatcattttg ttagaaataa gaaaatttat
    28261 ggttaaataa tttggtgatt atgaagcatt aaatcgtaat gccaaatatt gttcgctttg
    28321 gagagtatta ttgcctgttt gtgtctgttg agcagaaaaa tcatctgaat ttgaatactt
    28381 ttattaggtg tgaataaatg aaaaaatcat taaaatcatt aaaatcatta aaatcattaa
    28441 aatcattaaa atcattaaaa tcattaactt atatgaatat ttcagagttg gagttcgaat
    28501 gttgacacct gagtatttcc tgctaaaaat agccctgctt tacggatggt tgtattgcaa
    28561 taacacgaaa gtagtaagag attaaaggta tctgtcagcg ataactctgc aagtgatgac
    28621 attttgtaaa ccaaataatg taaatcagca atgtatcttt tcacttaatt ataacaaatg
    28681 tggtattcac taagaatctg atacgagctt acatcacgac tattttagtc tcatcgcggg
    28741 atacagggat gcaccgatgg atctcagcac accgtaatgc aatacctgaa caaatagaac
    28801 agtaaagtgg ttcagtatta atgaaagctc ttattttccg ttgggaaaac ttaaaataga
    28861 tgagaaggct ttttgatttc ttatgtgatc tcatttcgag tattgagaaa tttgtgattt
    28921 ttaaattttt caataagccc tgtaagggat tggggtttta tgagaaataa agagacgtat
    28981 tgtctttgtt taa
//
LOCUS       DBKELA010000049        28686 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.049, whole genome shotgun sequence.
ACCESSION   DBKELA010000049 DBKELA010000000
VERSION     DBKELA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.049"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..629
                     /gene="coaBC"
                     /locus_tag="ACUQR0_002093"
     CDS             <1..629
                     /gene="coaBC"
                     /locus_tag="ACUQR0_002093"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HHV9109130.1"
                     /translation="REPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSLPT
                     PPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDEL
                     TIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQP
                     TQGFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRYDEKNRR"
     gene            607..1065
                     /gene="dut"
                     /locus_tag="ACUQR0_002094"
     CDS             607..1065
                     /gene="dut"
                     /locus_tag="ACUQR0_002094"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HHV9109131.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            1172..1768
                     /gene="slmA"
                     /locus_tag="ACUQR0_002095"
     CDS             1172..1768
                     /gene="slmA"
                     /locus_tag="ACUQR0_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HHV9109132.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(1805..2446)
                     /gene="pyrE"
                     /locus_tag="ACUQR0_002096"
     CDS             complement(1805..2446)
                     /gene="pyrE"
                     /locus_tag="ACUQR0_002096"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HHV9109133.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(2512..3228)
                     /gene="rph"
                     /locus_tag="ACUQR0_002097"
     CDS             complement(2512..3228)
                     /gene="rph"
                     /locus_tag="ACUQR0_002097"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HHV9109134.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            3355..4218
                     /locus_tag="ACUQR0_002098"
     CDS             3355..4218
                     /locus_tag="ACUQR0_002098"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HHV9109135.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4439..5263
                     /gene="dinD"
                     /locus_tag="ACUQR0_002099"
     CDS             4439..5263
                     /gene="dinD"
                     /locus_tag="ACUQR0_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HHV9109136.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            5554..6171
                     /locus_tag="ACUQR0_002100"
     CDS             5554..6171
                     /locus_tag="ACUQR0_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HHV9109137.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(6168..7850)
                     /gene="ligB"
                     /locus_tag="ACUQR0_002101"
     CDS             complement(6168..7850)
                     /gene="ligB"
                     /locus_tag="ACUQR0_002101"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HHV9109138.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            8108..8731
                     /gene="gmk"
                     /locus_tag="ACUQR0_002102"
     CDS             8108..8731
                     /gene="gmk"
                     /locus_tag="ACUQR0_002102"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HHV9109139.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8786..9061
                     /gene="rpoZ"
                     /locus_tag="ACUQR0_002103"
     CDS             8786..9061
                     /gene="rpoZ"
                     /locus_tag="ACUQR0_002103"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HHV9109140.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            9080..11188
                     /gene="spoT"
                     /locus_tag="ACUQR0_002104"
     CDS             9080..11188
                     /gene="spoT"
                     /locus_tag="ACUQR0_002104"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HHV9109141.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            11195..11884
                     /gene="trmH"
                     /locus_tag="ACUQR0_002105"
     CDS             11195..11884
                     /gene="trmH"
                     /locus_tag="ACUQR0_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HHV9109142.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            11890..13971
                     /gene="recG"
                     /locus_tag="ACUQR0_002106"
     CDS             11890..13971
                     /gene="recG"
                     /locus_tag="ACUQR0_002106"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HHV9109143.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(14005..15210)
                     /gene="gltS"
                     /locus_tag="ACUQR0_002107"
     CDS             complement(14005..15210)
                     /gene="gltS"
                     /locus_tag="ACUQR0_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HHV9109144.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            15490..16881
                     /gene="xanP"
                     /locus_tag="ACUQR0_002108"
     CDS             15490..16881
                     /gene="xanP"
                     /locus_tag="ACUQR0_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HHV9109145.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17002..18711
                     /locus_tag="ACUQR0_002109"
     CDS             17002..18711
                     /locus_tag="ACUQR0_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HHV9109146.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(18764..21082)
                     /gene="yicI"
                     /locus_tag="ACUQR0_002110"
     CDS             complement(18764..21082)
                     /gene="yicI"
                     /locus_tag="ACUQR0_002110"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HHV9109147.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(21092..22475)
                     /locus_tag="ACUQR0_002111"
                     /pseudo
     CDS             complement(21092..22475)
                     /locus_tag="ACUQR0_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            22768..22862
                     /locus_tag="ACUQR0_002112"
     tRNA            22768..22862
                     /locus_tag="ACUQR0_002112"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22802..22804,aa:Sec,seq:tca)
     gene            23134..24347
                     /locus_tag="ACUQR0_002113"
                     /pseudo
     CDS             23134..24347
                     /locus_tag="ACUQR0_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            24759..25802
                     /locus_tag="ACUQR0_002114"
     CDS             24759..25802
                     /locus_tag="ACUQR0_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHV9109148.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(25946..26116)
                     /locus_tag="ACUQR0_002115"
                     /pseudo
     CDS             complement(25946..26116)
                     /locus_tag="ACUQR0_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            26317..26745
                     /locus_tag="ACUQR0_002116"
                     /pseudo
     CDS             26317..26745
                     /locus_tag="ACUQR0_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(26775..27926)
                     /locus_tag="ACUQR0_002117"
                     /pseudo
     CDS             complement(26775..27926)
                     /locus_tag="ACUQR0_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            28045..28314
                     /locus_tag="ACUQR0_002118"
                     /pseudo
     CDS             28045..28314
                     /locus_tag="ACUQR0_002118"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            28388..28645
                     /locus_tag="ACUQR0_002119"
     CDS             28388..28645
                     /locus_tag="ACUQR0_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109149.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT         7268 a         7498 c         7066 g         6854 t
ORIGIN      
        1 cgcgtgaacc gctcgatccg gtgcgttata tctctaatca cagctccggc aagatgggtt
       61 ttgctatcgc cgccgctgct gcccgtcgtg gcgcgaacgt cacgctggta tcaggtccag
      121 tttcactacc gacgccaccg tttgttaaac gtgttgatgt gatgaccgcg ctggaaatgg
      181 aagccgccgt gaatgcttct gtacagcagc aaaatatttt tatcggttgc gccgccgtgg
      241 cggattatcg cgcagctacc gtggccccag aaaaaatcaa aaagcaggcc acgcagggtg
      301 atgaattaac aataaaaatg gttaaaaacc ccgatatcgt cgcaggcgtt gccgcactaa
      361 aagaccatcg accctacgtc gttggatttg ccgccgaaac aaataatgtg gaagaatacg
      421 cccggcaaaa acgtatccgt aaaaaccttg atctgatctg cgcgaacgat gtttcccagc
      481 caactcaagg atttaacagc gacaacaacg cattacacct tttctggcag gacggagata
      541 aagtcttacc gcttgagcgc aaagagctcc ttggccaatt attactcgac gagatcgtga
      601 cccgttatga tgaaaaaaat cgacgttaag attctggacc cgcgcgttag gaaggaattt
      661 ccgctcccga cttatgccac ctctggctct gccggacttg acctgcgtgc ctgtctcgac
      721 gacgccgtag aactggctcc gggtgacact acgctggttc cgaccgggct ggcgattcat
      781 attgccgatc cttcactggc ggcaatgatg ctgccgcgct ccggattggg acataagcac
      841 ggtatcgtgc ttggtaacct ggtaggattg atcgattccg actatcaggg ccagttgatg
      901 atttccgtgt ggaaccgtgg tcaggacagc ttcaccattc aacctggcga acgcatcgcc
      961 cagatgattt ttgttccggt agtacaggct gaatttaatc tggtggaaga tttcgacgcc
     1021 accgaccgcg gtgaaggcgg ctttggtcac tctggtcgtc agtaacacat acgcatccga
     1081 ataacgtcat aacatagccg caaacatttc gtttgcggtc atagcgtggg tgccgcctgg
     1141 caagtgctta ttttcagggg tattttgtaa catggcagaa aaacaaactg cgaaaaggaa
     1201 ccgtcgcgag gaaatacttc agtctctggc gctgatgctg gaatccagcg atggaagcca
     1261 acgtatcacg acggcaaaac tggccgcctc tgtcggcgtt tccgaagcgg cactgtatcg
     1321 ccacttcccc agtaagaccc gcatgttcga tagcctgatt gagtttatcg aagatagcct
     1381 gattactcgc atcaacctga ttctgaaaga tgagaaagac accacagcgc gcctgcgtct
     1441 gattgtgttg ctgcttctcg gttttggtga gcgtaatcct ggcctgaccc gcatcctcac
     1501 tggtcatgcg ctaatgtttg aacaggatcg cctgcaaggg cgcatcaacc agctgttcga
     1561 gcgtattgaa gcgcagctgc gccaggtatt gcgtgaaaag agaatgcgtg agggtgaagg
     1621 ttacaccacc gatgaaaccc tgctggcaag ccagatcctg gccttctgtg aaggtatgct
     1681 gtcacgtttt gtccgcagcg aatttaaata ccgcccgacg gatgattttg acgcccgctg
     1741 gccgctaatt gccgcgcagt tgcagtaata tgacgccgga tgacttttca tccggcgagt
     1801 ttctttaaac gccaaactct tcgcgatagg ccttaaccgc cgccagatgt tccgccattt
     1861 ccggcttctc ttccagatag gcaatcagat ctttcagggt gatgatagag atcactttgc
     1921 agttgtaatc acgctcaact tcctgaatcg ccgaaatctc gccgcgcccg cgttcctgac
     1981 gatcgagcga aatcaacacg ccagcaagcg tcgcgccatt ggcctgaata atctccatcg
     2041 actcgcgaat cgccgttccg gcggtgatca catcatctac cagcattacg cgtccttgta
     2101 acgcgctacc aaccagattg ccgccttcac cgtggtcttt tgcttctttg cggttaaagc
     2161 agtacggcag gtccaggtcg tgatgctccg ccagtgccac agcggttgtg gtggcaatcg
     2221 ggatcccttt gtaagcaggg ccaaacagca gatcgaactc aatgccggaa tccaccaacg
     2281 cttcagcgta aaaacggcct aacagtgcca gatcgcgccc ggtattaaac agcccggcgt
     2341 tgaagaaata ggggcttttg cgcccggatt tcagcgtaaa ctcgccaaac tttaacacct
     2401 gcttgctaag cgcaaattca ataaactggc gctgatatgg tttcatgcct tcgctcctca
     2461 tcttactttt ctacagacaa aaaaaaggcg actcatcagt cgccttaaaa atcagtttgc
     2521 cagcgccgcc ttctgcgtcg ctacaatgga ttcgattccc cctcgggcca gcgccaacaa
     2581 ggtgagtagc tcttcatggg tgaacggctc gccttctgcc gtcccctgca cttcaatgat
     2641 gcgcccgtct tcggtcatca ctacgttcat gtcggtctct gcggctgagt cttcaacgta
     2701 ttccagatcg caaattgctt caccgtttac aataccgaca gaaactgcgg ctaccatccc
     2761 tttcatcggg ttggttttca gcttgccgtt ttccaccagc ttctgtagcg catctgccag
     2821 cgccacgcag gcacccgtaa tcgacgcggt acgagtgcca ccatcagcct gaagcacgtc
     2881 gcagtccagc gtaatggtga actcacccag cgctttcaaa tctactgccg cgcgaagagc
     2941 acgggcgatc agacgctgga tttccattgt gcgtccaccc tgcttacctt tcgccgcttc
     3001 acgagcgtta cgggtgtggg tagaacgtgg cagcatgccg tactctgcgg tgatccagcc
     3061 ctggccctga cctttcagga agcgcggcac gccttcttca atagaggcgg tacacaatac
     3121 tttggtatcg ccaaattcga ccagcaccga gccttctgca tgttttgtat agttacgagt
     3181 cagggtaacg ggacgcacct gattattgct acggcctgct ggacgcatat tgaaatctcc
     3241 ggcttgaaac aaatgtggct gcgcattata cggacttccg gcggttattc ctatcctgac
     3301 aaggcatcga tggctataat ccttccacct ctccttttat aaacaggaac gtctatgatc
     3361 cgcagtatga ccgcctacgc ccggcgtgaa atcaagggtg aatgggggag cgcaacctgg
     3421 gaaatgcgct cggtaaacca gcgttatctg gaaacttact ttcgtctgcc ggagcagttc
     3481 cgtagccttg aacctgtcgt tcgcgagcgt attcgttctc gcctgacgcg cggtaaagtg
     3541 gaatgtaccc tgcgctatga gccagatgtt agcgcacaag gtgagctgat cctcaatgaa
     3601 aaactggcta aacagctggt aactgccgcg aactgggtaa aaatgaagag tgacgaaggg
     3661 gaaatcaacc cggttgatat tctacgctgg ccgggcgtga tggcagccca ggagcaggat
     3721 ctcgacgcga ttgccgctga aattctcgcg gcgttggatg gtacgctgga cgactttatc
     3781 gtcgcgcgcg aaaccgaagg tcaggcactg aaagcattga tcgagcagcg tctggaaggc
     3841 gtcaccgccg aagtggtcaa agtccgcgcc catatgccgg aaattctgca atggcagcgt
     3901 gagcgtctgg tcgcgaagct ggaagatgct caggttcagt tggaaaacaa ccgtctggag
     3961 caggagttgg ttctgctggc acaacgaatt gacgttgccg aagagctgga ccgcctcgaa
     4021 gcgcatgtta aagagacgta caacattctg aagaaaaaag aagcggttgg tcgtcgtctg
     4081 gactttatga tgcaggagtt caaccgtgag tcgaacactc ttgcgtcgaa atctatcaat
     4141 gccgaagtga caaactccgc catcgagctg aaagtgttga ttgagcagat gcgcgagcag
     4201 attcagaata ttgagtaact cgtaacacca attctgactt ttcagccttc acaaatgccg
     4261 cccactcaaa cagagcggca tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg
     4321 ctccagattt tgcgatgttt ttttgcccag cacactgaga acgtgagata ctcacaactg
     4381 tatataaata cagttacaga tttactttct ttgcaatcga tatcacatgg agtgggcaat
     4441 gaacgaacat catcaacctt ttgaagagat aaaactgatt aatgcaaacg gagcagaaca
     4501 atggtcagca agacaacttg ggaaactact gggttattca gagtatcgtc actttatacc
     4561 tgtattaacg cgcgccaaag aggcctgtga gaacagtggt cacacaattg atgaccattt
     4621 cgaggagatc ctcgatatgg tcaaaattgg ctcaaatgcc aaaagagcat taaaagacat
     4681 cgtactctcc cgctatgcct gttacctggt agtacaaaac ggcgaccccg cgaaaccggt
     4741 cattgcggca gggcagactt attttactat ccagacccga cggcaggagc ttgctgacga
     4801 tgaagcattt aagcaacttc gtgaagacga aaagcgtctg tttctgagaa atgagttgaa
     4861 agaacataac aaacaattgg ttgaggcagc acagcaagct ggggtagcga cagctactga
     4921 tttcgccata tttcagaatc atggttacca ggggctgtat ggtggattag atcagaaagc
     4981 catccaccaa ctgaaggggc tgaaaaagag tcagaagatc ctggatcata tgggttcaac
     5041 agaactggcg gctaatctct ttcgagctac ccaaacagaa gaaaaactca agcgggatag
     5101 cgtgaattca aaacagcaag ctaataccac acactttgac gtgggtagca aggtgaggca
     5161 aaccattcag gaacttggcg gaaccatacc tgaggagttg ccgaccccgc aggtcagcat
     5221 caagcagttg gaaaacagtg taaaaattac agagaagaaa tagcagaaac gaaaaactct
     5281 ttttgttgat tgagacaccc gatgcgtaag ccaaggtcca ggtgcagtga acacaatggc
     5341 taaacattgc acctttcttt tcccccctca gttttaacct attttttctt atgcattttc
     5401 tcagacaaga agtcagaaga atgcatctac gctacagaaa atagcgattt cacataacta
     5461 aaagttatca atttcctctc accttaaaaa atatcaatcg tgacaatgcg cacaaatcgc
     5521 taccctgcca gacagatttt tagggagaga accatgctgt tacacatttt gtatttggtt
     5581 ggcattactg ccgaagccat gacgggggcg ctggcggccg ggcgacggcg catggataca
     5641 tttggcgtaa ttattattgc aaccgccacc gcaattggcg gagggtcagt gcgcgatatt
     5701 ctgctgggcc actatccgct cggctgggtc aaacacccgg aatatgtgat tatcgtcgct
     5761 accgccgcag tactcaccac tatcgttgct cctgtaatgc cttacctgcg caaagtgttt
     5821 ctggtgctcg atgcgctcgg actggtggtc ttttctatca tcggcgcaca ggttgcactg
     5881 gatatggggc acggcccaat tattgccgtt gtcgcggcgg taaccaccgg tgtatttggc
     5941 ggcgttttgc gcgatatgtt ctgtaaacgc attccgctgg tctttcaaaa agagttgtat
     6001 gccggtgtct catttgcctc cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac
     6061 catgatgtgg tgattatctc caccctggta ttcggctttt ttgctcgttt actggcgcta
     6121 cgtcttaagc tgggattacc ggtcttttac tacagccacg aaggtcacta aggttcaaaa
     6181 cctgtgatct gctgggcagc cagccaactg cccagcttct tgatttgcgc attttccttc
     6241 cattcaataa cctgtctggc gcgcccagat ccagtccccg gcagctgctg ccagaactgc
     6301 tccgtgctaa ataaaagttg cgaccaggac cgttcatcac tggcattaag cgccgcccgg
     6361 gttagcggta ttcccattgc catcacccag cgagtaaaag gctggttacg agccagatta
     6421 aactgatgcc ataactgcgc acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc
     6481 tctggcgtta ataaaagcca ggaaaagata tgctcaaagc gatgagtctg atgcagcgcg
     6541 cgccaaccgg cctcaccaat gccatccagc cccagaacct gttttgaccc cagccagact
     6601 aagcgtgaaa tgaactgttc ctgacaaaca tcagaagcaa agtagcaggt caacgggtta
     6661 aagcggtttt ctggtggtgt cggttttgta cgttctgcac cgcgccagac cacatcatca
     6721 atgcgaggaa taccctgacc ggcaaggctg acgaggatct gatcaccagg cgcaatatcc
     6781 cactcctgcc agcgcctgac ggaaccaata ttcacccgct ggactttttt atcatccagc
     6841 atgacaggtg cgagtgacgc aaccaccgat attttaccgc tcttacccac cgcaaactga
     6901 atcgccttca cttcggcaac ctgagctaca ggttgatatt tccaggccac cagccactcc
     6961 gcctggcccg gtagccaatg acgggattcg ggttccttag cccctcgcac aaccacgcca
     7021 tcggtgacga agggtaattt cgctttccac cactcattgc gtacgcgcgc aacctcatca
     7081 gcatttttaa ccgcacgggt atacctctgc gttagagtaa aacctgcggt agccagctcc
     7141 tttaaacgat cggtcattaa ctgcggtcca tccggccatg cccagacaaa aacagccagt
     7201 gagtttagcg tgtcgctatt cccctggcgc atcatcaggc cagcaacttt tgcgcgggca
     7261 tttattcccc ccatttgttg ttggatatgc ccctcgcgct ggagaaatat ttccccctga
     7321 agcgtactgt tggctaaagg cccgctaacg gtttgcggca cagcggaaat taagctaact
     7381 ttctgcgtcc agtcctcgcc tttcaggcca ttaccgcgac tgattgcttt gttcagtttc
     7441 ccgtcccgat aaaccagggt taccgcaacg ccatcaactt ttggctgcac ccaaagatcg
     7501 ctacgttctc gcatccacag acttaatgcg tttttatccg ccattttgcg tacacccgta
     7561 tgggcaaccg gatgcataac cgcaccattt aaaggcggca tcatcacgtc gcggggctcg
     7621 ctcacaaagc agcgttgcca ctgcgtaaga cgggcgctta actgatcgta aacaccgtct
     7681 tccacctcac tttttccttc cttccagtag tcatcgtccc actgttttat ttgctgttgc
     7741 aggcgggaaa tttcttcctg tgctctggct ggcgaccagg ccggacagac cgcccacaca
     7801 gatgattgcc agcacaagat acttattaat atcgccatcc atactttcat cttcacctcc
     7861 gctgtagtca ggctggcaga tatacaacgt ggtgaaggca aagccgagtg gcaaaaacgg
     7921 agtctgcgag gacgcttcct gagaatcgtc tttattgcag taaatcacag gcaaatgcgg
     7981 aaacagctac gcaaaacgca acaactttgc gcaaaaagtg tgagcaaggg ctacgtcaca
     8041 tggccgcgcc gtgtataata agctcgtatg taggctttat ttcgctaatc acatacgaaa
     8101 gatactcatg gctcaaggca cgctttatat tgtttctgcc cccagtggcg cgggtaaatc
     8161 cagcctgatt caggctttat taaaaaccca accgttgtat gacacccagg tttctgtttc
     8221 acacaccaca cgccaaccgc gtcctggtga agtccacggt gaacattatt tctttgttaa
     8281 tcatgatgaa tttaaagaaa tgattagcag agatgcgttc ctcgaacacg cagaagtttt
     8341 tggtaattac tatggcactt cgcgtgaggc cattgagcaa gtactggcga ccggtgtcga
     8401 tgtttttctc gatatcgact ggcagggcgc gcagcaaatt cgccagaaga tgccgcacgc
     8461 gcggagtatc tttattttac cgccgtccaa aattgaactg gaccgccgtc tacgcggtcg
     8521 cggtcaggac agcgaagagg tcattgcaaa gcgtatggcg caagctgttg cagaaatgag
     8581 ccattacgcc gaatatgatt atctgattgt gaatgatgac ttcgataccg cgttgaccga
     8641 tttgaagacc attattcgcg ccgaacgtct gcgcatgagc cgccaaaagc agcgtcatga
     8701 cgctttaatc agcaaattgt tggcagactg aacctgattt cagtatcatg cccagtcatt
     8761 tcttcacctg tggagctttt taagtatggc acgcgtaact gttcaggacg ctgtagagaa
     8821 aattggtaac cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt
     8881 aggcggaaag gatccgctgg taccggaaga aaacgataaa accactgtaa tcgcgctgcg
     8941 cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac gttcgcgaac gccaggaaca
     9001 gcaagagctg gaagccgctg aattacaagc cgttaccgct attgctgaag gtcgtcgtta
     9061 atcacaaagc gggtcgccct tgtatctgtt tgaaagcctg aatcaactga ttcaaaccta
     9121 cctgccggaa gaccaaatca agcgtctgcg gcaggcgtat ctcgttgcac gtgatgctca
     9181 cgaggggcaa acacgttcaa gcggtgaacc ctatatcacg cacccggtag cggttgcctg
     9241 cattctggcc gagatgaaac tcgactatga aacgctgatg gcggcgctgc tgcatgacgt
     9301 gattgaagat actcccgcca cctaccagga tatggaacag ctttttggta aaagcgtcgc
     9361 cgagctggta gagggggtgt cgaaacttga taaactcaag ttccgcgata agaaagaggc
     9421 gcaggccgaa aactttcgca agatgattat ggcgatggtg caggatatcc gcgtcatcct
     9481 catcaaactt gccgaccgta cccacaacat gcgcacgctg ggctcacttc gcccggacaa
     9541 acgtcgccgc atcgcccgtg aaactctcga aatttacagc ccgctggcgc accgtttagg
     9601 tatccaccac attaaaaccg aactcgaaga gctgggtttt gaggcgctgt atcccaaccg
     9661 ttatcgcgta atcaaagaag tggtgaaagc cgcgcgcggc aaccgtaaag agatgatcca
     9721 aaaaatcctc tcggaaatcg aagggcgttt gcaggaagcg ggaattccct gccgcgtcag
     9781 tggtcgcgag aagcatcttt attcgattta ctgcaaaatg gtgctcaaag agcagcgttt
     9841 tcactcaatc atggacatct acgctttccg cgtgatcgtc aatgattccg acacctgtta
     9901 tcgcgtgctc gggcaaatgc atagcctgta caagccgcgt ccgggtcgcg tgaaagacta
     9961 catcgccatt ccaaaagcga acggctatca gtctttgcac acctcgatga tcggcccgca
    10021 cggtgtgccg gttgaggtcc agatccgtac cgaagatatg gatcagatgg cggagatggg
    10081 tgttgccgcg cactgggctt ataaagagca cggcgaaacc agcaccaccg cgcaaatccg
    10141 cgcccagcgc tggatgcaaa gcctgctgga gctgcaacag agcgccggta gttcgtttga
    10201 atttatcgag agcgttaaat ccgatctctt cccggatgag atttacgttt tcacaccgga
    10261 agggcgaatt gtcgagctgc ctgccggtgc aacgcccgtc gacttcgctt atgcagtgca
    10321 taccgatatc ggtcatgcct gcgtgggcgc acgcgttgac cgccagcctt acccgctgtc
    10381 gcagccgctt accagcggtc aaaccgttga aatcattacc gcaccgggcg ctcgcccgaa
    10441 tgccgcgtgg ctgaactttg tcgttagctc aaaagcgcgc gccaaaattc gtcagttgct
    10501 gaaaaacctc aagcgtgatg attctgtaag cctgggccgt cgtctgctca accatgcttt
    10561 gggtggtagc cgtaagctga atgaaatccc gcaggaaaat attcagcgcg agctggatcg
    10621 catgaagctg gcaacgcttg acgatctgct ggcagaaatc ggacttggta acgcaatgag
    10681 cgtggtggtc gcgaaaaatc tgcaacatgg ggacgcctcc attccaccgg caacccaaag
    10741 ccacggacat ctgcccatta aaggtgccga tggcgtgctg atcacctttg cgaaatgctg
    10801 ccgccctatt cctggcgacc cgattatcgc ccacgtcagc cccggtaaag gtctggtgat
    10861 ccaccatgaa tcctgccgta atatccgtgg ctaccagaaa gagccagaga agtttatggc
    10921 tgtggaatgg gataaagaga cggcgcagga gttcatcacc gaaatcaagg tggagatgtt
    10981 caatcatcag ggcgcgctgg caaacctgac ggcggcaatt aacaccacga cttcgaatat
    11041 tcaaagtttg aatacggaag agaaagatgg tcgcgtctac agcgccttta ttcgtctgac
    11101 cgctcgtgac cgtgtgcatc tggcgaatat catgcgcaaa atccgcgtga tgccagacgt
    11161 gattaaagtc acccgaaacc gaaattaatg ttttatgaac ccaacacgtt atgcacgcat
    11221 ctgcgaaatg ctcgccaggc ggcagcctga tctgaccgtc tgcatggagc aggtccacaa
    11281 acctcataac gtttctgcga ttattcgtac cgcagatgcc gttggcgtac atgaagttca
    11341 cgccgtctgg cctggtagcc gcatgcgcac catggcttcg gcagcggcgg gtagtaacag
    11401 ctgggtacag gtgaaaacac accgcaccat tggcgatgcc gtcgctcatc tcaaaggcca
    11461 gggcatgcag attctggcaa cccatctttc tgataatgct gtcgatttcc gcgaaattga
    11521 ttacactcgc ccgacctgca ttttgatggg acaggagaaa acgggcatca cgcaggaagc
    11581 attggccctg gcggatcagg acatcatcat tccgatgatc ggcatggtgc agtcgctgaa
    11641 tgtttccgtt gcctcagccc tcattcttta cgaagcccag cgtcagcggc aaaatgcagg
    11701 catgtacctg cgtgaaaaca gcatgttgcc ggaagcagag caacaacgcc tgttgtttga
    11761 aggcggctat ccggtgctgg cgaaagtcgc aaaacgcaaa ggcctgcctt atccccacgt
    11821 caatcagcaa ggcgagatcg aagctgatgc cgactggtgg gctactatgc aggctgcagg
    11881 gtaagtgcca tgaaaggtcg cctgttagat gctgtcccac tcagttccct aacgggcgtt
    11941 ggcgcagcac ttagtaacaa actggcgaaa atcaacctgc ataccgtgca ggatctactc
    12001 ttacaccttc ccctgcgcta cgaagatcgc acccatctct accccatcgg agaactactg
    12061 ccgggcgttt atgccacggt ggaaggcgaa gtgctgaact gcaatatctc cttcggcggt
    12121 cggcggatga tgacctgcca gatcagcgac ggttccggca tcctcaccat gcgctttttc
    12181 aatttcagcg cggcaatgaa aaatagcctg gcgacgggcc gccgtgtact ggcttatggc
    12241 gaagcaaagc gcggtaaata tggtgcggag atgatccacc cggaataccg cgtgcagggc
    12301 gatctcagca cgccagaatt acaggaaacg ctcacgccgg tttatccaac aacggaaggc
    12361 gtaaagcagg ccacgctgcg taaattaacc gaccaggcgc tggatctgct cgacacctgc
    12421 gccattgaag aactcctgcc gccggaactg tcacaaggaa tgatgacgct accggaagcg
    12481 ttgcgcactt tgcaccgccc gccaccgacg ctacagctta gcgatctgga aaccgggcag
    12541 catccggcgc aacgtcgtct gattctggaa gaactgctgg cgcacaacct cagcatgtta
    12601 gccttacgtg ccggagcaca gcgttttcat gcccagccgc tgagcgccaa tgacacgctg
    12661 aaaaataaac tcctcgccgc cttaccgttc aagccaacgg gcgcacaggc acgcgtagtg
    12721 gcggagatcg agcatgatat ggcgctggat gtgccgatga tgcgcctggt acagggcgat
    12781 gtaggttccg gtaaaacgct ggtcgccgcc ctcgccgcgc tgcgtgcgat tgcccacggc
    12841 aaacaggtgg cactgatggc accaaccgaa ttactcgccg agcagcacgc caataacttc
    12901 cgcaactggt ttgcaccgct cggtatcgaa gtgggctggc tcgccggtaa gcagaaaggc
    12961 aaagcgcgac tgtctcagca ggaagccatc gccagcggtc aggtgcagat gattgtcggt
    13021 acacacgcca tcttccagga acaggtgcag tttaacggcc tggcgctggt gattatcgac
    13081 gaacagcatc gttttggcgt gcatcagcgt ctggcattgt gggagaaagg ccagcagcag
    13141 ggcttccatc cgcatcagtt gatcatgacc gccacgccga tcccccgcac gctggcaatg
    13201 acggcgtatg ccgatctcga tacctcggtg atagatgagc tgccgccagg ccgcacgcca
    13261 gtgactacgg tcgctattcc tgatacccgc cgtaccgaca tcattgaccg cgtgcgccac
    13321 gcctgcataa ctgaaggtcg tcaggcgtac tgggtttgta cgttgattga agagtcagag
    13381 ttactggaag cgcaggcggc ggaagctacc tgggaagagt tgaaactggc gctaccagaa
    13441 ttgaatgttg gcctggtaca cgggcggatg aaaccagccg agaaacaggc ggtgatggcg
    13501 tcgtttaaac aaggtgagct acatctgctg gttgccacta ccgttattga agtcggtgtt
    13561 gatgtgccta acgccagcct gatgattatc gaaaaccctg agcgtctggg tctggcgcag
    13621 ttacaccagc tgcgcgggcg cgtaggtcgt ggcgcggtgg cttctcactg cgtgctgctc
    13681 tacaaaacgc cgctttctaa aacggcgcaa attcgcctgc aagtgctgcg cgacagtaac
    13741 gacggttttg tgattgcgca aaaagatctg gagattcgcg gccctggcga attgttaggc
    13801 acgcgtcaga cgggtaatgc tgaatttaaa gtggcggatt tactgcgcga tcaggcgatg
    13861 atcccggaag ttcagcgcct ggcgcgacat attcacgaac gttacccaca acaggcaaaa
    13921 gccttgatag aacgctggat gccggagaca gaacgttact cgaatgcgta aaaggcggcg
    13981 gtgagaagac cgccgcttca tcggttaacc ggcaaaaatc ggcaacatta aatacagctt
    14041 aatcaccagc gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa
    14101 cgccatgtgc gacgggccaa agcgttcagt gatcgcctgc atgttggcga ttgccgttgg
    14161 cgttgcaccg aggccaaaac cacagtgacc cgcagccagc actgccgcat cgtagttttt
    14221 gcccatcatg cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac
    14281 cagaatcgcc agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa
    14341 cgccatcgcc aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa
    14401 gacgcggtaa aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac
    14461 acagacgaag gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc
    14521 caccgtcagg cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat
    14581 gcgtcccaca tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt
    14641 cggcgtggtg gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag
    14701 caccagaccg aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg
    14761 ttcaatgaac aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga
    14821 accggccaac agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat
    14881 ggcattttgc atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc
    14941 accggcacgc aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat
    15001 taacggatcg cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac
    15061 tagtagcgcc agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt
    15121 caaaaaggag acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac
    15181 aagcgttgct aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt
    15241 catactcgtc atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt
    15301 atgctttcct gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga
    15361 aaaataccaa aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat
    15421 gtggcaaacg tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga
    15481 ttgttttcga tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgctcagact
    15541 caaaacagcg aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt
    15601 gccgcctgtc agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc
    15661 tgccaggcgc tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt
    15721 gcctccggtg tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg
    15781 ttgtctattc agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg
    15841 ctgaaaaccg gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg
    15901 ctggcaagtt gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt
    15961 acgccgctgg tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg
    16021 ctgacctcca ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa
    16081 aatctgctgc tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac
    16141 ccttacttac gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg
    16201 tttatgggca tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca
    16261 acgccgctct attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc
    16321 tttatgatca cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa
    16381 cagccagtgt ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc
    16441 ctgaactcgt ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac
    16501 aacggagtga tccagttgac tggtgttgcc agccgctatg tcggttttgt cgtcgcgctg
    16561 atgttgatcg tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa
    16621 ccggttctgg gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt
    16681 atcgtttctc gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg
    16741 gttggtctgg gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa
    16801 aacctgctct cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt
    16861 ttcccaccag aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca
    16921 cctctttcac cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg
    16981 catttcacgg gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc
    17041 gctctgttag tggtgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa
    17101 catatcagcg catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggat
    17161 caccgttttt ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggt
    17221 cagcccgcgc ccctggtggc aaaaagtgtc gacattgcgc taagcagtcg ccaactgacc
    17281 gaaccacgcc atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag
    17341 accgcgccgc taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc
    17401 ccgaatagcg aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca
    17461 ccagaagccg gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt
    17521 tcgctcaatg atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc
    17581 gttacgctga ctaacctggg tgccgacatc gcccgcggga cattaaccgg aaacgcgcag
    17641 cgtaacgccg acggcagctg gcaagtggaa aatctgcgca tggcggatat ccgtctacaa
    17701 agcgaaaaat cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt
    17761 ggtcgcttgg aagtcatcga tgctcgtctg caaggtccgg actgggcggt gaccgacctc
    17821 gatctcagct tgcgcaacat gaccttcagt aaagatgact ggcagacaca ggaaggcaaa
    17881 ctgtcgatga acgccagcga gttcatttat ggttcgctgc atttatttga cccgattata
    17941 aacgcggaat tttccccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg
    18001 ggtatggtca gaacgtcagg gaactggctg cgtgacggta aaacgttgat ccttgatgat
    18061 gcggcaattg ccgggctgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa
    18121 acgacacccg gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg
    18181 atcattgata tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc
    18241 aacctgacgc tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat
    18301 gccgccgccg cgacattcaa tcgtgttgat gttcgtcgcc cgtcgctggc gctgaccgcc
    18361 aacagcagca cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa
    18421 gccaccgcaa gtgtttcaca aacgccacaa cgtcagaccc atatcagcct gaatggacgc
    18481 ggtgtgccgg tgaatatttt gcaacaatgg ggatggcctg aattaccgtt gactggcgac
    18541 ggcaatattc agcttaccgc cagtggagat attcaggcca atgccccgct gaaacctacg
    18601 gttagcgggc aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat
    18661 gcgggcgtcg tttccagcgg tgaagttaca tcgacggagc cggtgcggta aacatcaatc
    18721 tcgccgggta cgactcagac gtacccggca ttccgtcaat agattacaac gtaattgtca
    18781 gcgcattccc ttgaggcgtc accaccagcc cctgctcact ttcagcctgc gaaccgcctt
    18841 gcagaccatt tactttcaca acattgcgca agcacagcgt ccagttcttc gcctcgcccg
    18901 caccagtcac agtaatcgtg ttgccagtac gtgctgcttt taaagtaaag atcaccgatc
    18961 cgtcagcagc aggcacttca cagacggctt catgcccgtc ttgcagatta aagaggtgga
    19021 atgccgtgcc ttcgtgccac acgtaatcgg gacgttgatc gttgttgccc agcgccagta
    19081 gagtgttatc acgcacataa acgggcagac tcaggaagcc gtgctgctgt ttatgccagc
    19141 gactaccgtc gagttcatcg ttgtgccaca ggtgtgtcca gcgaccttcc ggcaggtaga
    19201 actgcacatc gcccgcttca gtgaacaccg gcgcaaccat cacgttgtcg cctaacatgt
    19261 attgacggtc aaggtaatca caagccggat cgtccgggaa ctccatcatc atggcccgca
    19321 tcatcggcgt accccgcgcg ttcgcacgcg cagcttcacg atacagatac ggcatcatgc
    19381 ggcatttcag ttgcgtgaag aagcgcacca catcacagga ctcatcatcg taggcccacg
    19441 gcacacgata agatttgcta ccgtgtaaac ggctatggct ggagagcaaa ccaaacgcgc
    19501 accagcgttt gtaaacgtgc gccggagcgg tattttcaaa gccgccgata tcgtggctcc
    19561 agaagccaaa acctgaaagg ccaatagaca aaccaccgcg caggctttcc gccattgatt
    19621 cgtagttagc gtaacaatcg ccaccccagt gtaccgggaa tttctgcgca ccgacggagg
    19681 ccgagcgggc aaacaagaca gcttcttcct caccaacggt gtccttgagc acgttccaca
    19741 ccagttcgtt gtagatgtac gcataatggt tatgcatttt ctgcggatcg gaaccgtcaa
    19801 accactgaac atcaattggg atacgttcgc caaagtcggt cttaaagcaa tcaacgccca
    19861 tcgcgaccag acctttcagt ttgtcggcgt accatttgca ggcatccgga ttggtaaagt
    19921 cataaatcgc cagacctggc tgccatttat cccactgcca tagcgaaccg tccgggcgtt
    19981 tgagtaaata gcctttctct tgtaactctt taaagacggg ggatttttga ccgatatagg
    20041 ggttaatcca gacgcagatt ttcagccctt tcgctttcag gcggcggatc atcccttccg
    20101 ggtcagggaa agtcagcggg tcccactcaa aatcgcacca ctggaaggct ttcatccaga
    20161 aacagtcaaa gtggaaaaca tgcagcggca gattgcgttc cgccatacca tcgataaagc
    20221 tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt ggttagccac aggccgaagg
    20281 accacggggg cggcagcgcc ggacgaccag taaagcgggt ataacgatca agtaccgctt
    20341 tcggcgtcgg gccgtcgata acaaagtatt cgagatattc actctcaacg ctgaactgca
    20401 ctttggagac tttctccgat cccacttcaa aagagacaca ctggggatga ttgaccagta
    20461 ccccataacc acggttagtc atgtagaacg ggatattttt atacgcctgt tcagtacttg
    20521 tgccgccgtc ccggttccag gtctctaccg tctggccatt gcgcaccagg gcagtaaagc
    20581 gctctcccag accgtaaact gtttcgccaa cgccaagatc aagccgctca aacatataat
    20641 tgcgttgatt attcgtgtcc tgcacgtagc cattattttt cacctgacta ccggtaatac
    20701 gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc tttgctgaca cgcgcgctta
    20761 agttgccact tttaaactca gcataacgtt ctgtgttttc gattgtgacc ttcacgtcct
    20821 gcaaaatatt gagcggataa tgaggaccgt tattcagcgc cccctgaaaa tgctcaatcc
    20881 gcacaccgac aataccttcc tgtggggaga aaaagcgcag cgtaaataaa ggcgtatcaa
    20941 gctgccaggt acgttcacgc acatcacggg gggcagcata gaccaccatt tcattatcct
    21001 gctgttcaac ctcgaatacc tgaagcgggt gaatcaaatt gaggccaggt tgaatcaacc
    21061 agtttccatc gctaattttc attgccgttc cttagttctg cacttcttga gaggtgaatt
    21121 gttgctggca acggcgttta ccctgagcca gctgttccat aacggttttc agattgtgcg
    21181 tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag caaataacag atcgccggaa
    21241 caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct gttctgcgct ttttctgccg
    21301 catcatatcc gccataagcc agcatccagc cgataagagc gccgccgaag gccaacccca
    21361 gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt caggcgttta ccattgcacc
    21421 actcgccgta gtcgacggta tcgaacatca ttacccactg gataggtgtc accagttgat
    21481 gcaacacacc aatcacgaag atgaagacaa acatagtgat gctggcctgc atgggaacaa
    21541 agaacatcgc gaggctaatc actgccagca gggcgttcgt ccaccagaag atagtgactt
    21601 tacatttcca gtcggtcaga ggttttgcca gtgcggaacc aatcaggtta cccacgcaat
    21661 aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa aatccatgtg acgtaataca
    21721 tcatcgcccc accgcgtacg cacaccgcca ggatattgaa aatggttagt aaaccgacaa
    21781 tccgccactg gtcgttttgc cagatatcac gtaaatcttc ccgcatagac gttgttgtag
    21841 atggtgcttc aacgcgttct ttagtggtga agaaacaaaa tgccagcatc atgaatgcca
    21901 ccacggaaag gaccacgata ccgccctgga aaccgagtgg tttattatca ccgccaatta
    21961 aattaaccag tggcatcatc agaacagtag aaagcatgcc tcccgcggtc gccagcacaa
    22021 aacgccagga ttgcagcgag atacgctgag tcgggtcatt ggtgattaca ccacccaatg
    22081 cgcagtaagg gatattgacg acggtataaa gtaaggtaag ttagggtgta agtaattgct
    22141 gcatagatca ttttgccgtt catactgaga tctggcgtgc tataggccag tacacagacg
    22201 atcccgaatg gcagtgcgcc aaacagtacc cacggacgaa atttacccca gcgagagcgc
    22261 gttcgatcgg ccaacaaccc catgcaagga tcggaaatcg catccagtgc acgagcgacc
    22321 aaaaacatgg ttccgacaaa tccggcagga atgccaaaaa tatcggtata aaagaacatc
    22381 atatataaca ttacgttatc gaaaataatg tggctggcgg cgtctcccat gccataacca
    22441 attttctctt taacggacaa cacttcactc ctcatctgct tttccttgat gctatagggg
    22501 tgctgagacc gataacgcat tttgtaaacc atccctgtga gagcgaatat tccgatatct
    22561 ggttattaat ttatgattct tgttttatgt gatcgtggta gcgttaattc cgctcatata
    22621 tcattgtaaa atatgggttt tatataaatt ataatgcttt cgtgataata cgctgcgttt
    22681 attaggcgga aaaaactgat ctgggggatg tagaaactca aggaagtagc tataatgcgc
    22741 cccgcctcca tgtagcaatc gaggcgcgga agatcgtcgt ctccggtgag gcggctggac
    22801 ttcaaatcca gttggggccg ccagcggtcc cgggcaggtt cgactcctgt gatcttccgc
    22861 cagtttacct cttctgaact ctcccaatgt caataaatct cttctaatcc cttgtatatc
    22921 agtatttatt tgtatcctga ggtcaaccta cttcaccccg tatcaagttt cttttggggg
    22981 tactttaggg ggtgttattt cctattgaaa tacaagcacc cccataatag gaaaaatgtg
    23041 atacataaca acggctttta acaatatctt tgttatacaa ggggtgtatt gggggtatca
    23101 ggttagttac tggcagaaat ccccctcagg aacatgaatt catcaaaaag ccggaggatg
    23161 tcatggcatt aaccgatatc aaagtgaaaa ccgcaaaacc caaagacaaa ccctataagc
    23221 ttgctgatgg cggcggtatg tatctactga ttaacactaa tggttctaaa tactggcgca
    23281 tgaagtaccg ctttgctggt aaagaaaaga tgctttctat tggcgtatac cccgatgtaa
    23341 cgctagccga tgctcgcgag aaacgcagtg aagccaggaa actcctcgct gctggaggcg
    23401 atccaaggga ggcaaaaaag gaagaaaaaa tcgcgcagca aatgagtctg aaaaatacgt
    23461 tcgaggctat tgctcgcgaa tggcaccagt taaaagctga tcggtggtca ttacgctatc
    23521 gtgatgaaat cattgatacc tttgaaaaag acatttttcc ctacattggc aagcgcccta
    23581 ttgccgaaat caaaccaatg aagttactgg aagcactgcg taaaatggaa aaacgtggag
    23641 ctctggagaa aatgcgcaag gtccgccagc gctgtggtga ggtgttccgc tatgcaattg
    23701 ttactggccg ggcagactat aacccagccc ccgatcttgc cagtgcttta gctacaccga
    23761 aaaaagtaca ttttcccttt cttactgcta atgaacttcc ccacttcctc aacgatctgg
    23821 cgggttatac cggaagtata atcactaaga cagctactca gatcattatg ttaaccggcg
    23881 tacgaacaca agaactgcgt tttgcacgct gggaagatat cgattttgag acaaaattgt
    23941 gggagatccc ggcagaagta atgaagatga agcgacctca tattgtgccg ctctctgagc
    24001 aggtcattat gctatttaag cagcttgaac caatctcaaa acatcatccc ctagtcttta
    24061 tcggtagaaa tgatccccgc aaaccaatta gtaaggaaag cattaaccaa gtaattgaat
    24121 tacttggata taagggaaga ctcacagggc acggtttcag acacaccatg agtacaattc
    24181 tgcatgaaca aggctttaat tccgcctgga ttgaaatgca gttagctcat gtggataaaa
    24241 attccatcag gggtacctat aatcatgccc tgtatctcga tggccgccgt gaaatgatgc
    24301 aatggtacgc tgattacatt gattcactct ccagccggga aagttaagtc agaaatactt
    24361 ttccctgatg ttttcctcaa ttaagtattt tatcctctgg cctacaaggc tggaggattt
    24421 ttgttattat attatttttt tgcgtcttca aacttattac aaaaacataa ttaaaaacaa
    24481 attgacattt ttaaattggc aattacaaaa tacagccttg cagttatttc gataccttta
    24541 gcaaaggaat agttgattca gatataattt agcatttcaa tcatctgaca tcattataaa
    24601 aaatgagatt cacataaaga taaaattatt gttctcaagg aaagcatgaa ggggcttcgt
    24661 aagcaatatt cattcggtgc caagacagga cttgatacac agaatctcaa cttttatgct
    24721 ctcagactat atcaccacat gttttacagg ctcagaaaat gaacgataga ttatgctttg
    24781 aagtgcacga taacaagggt tatttcgtat ttccggatac ctggtttggc ccattgctgg
    24841 gggagtttga agaagtgctt gatgcatacg acgctgatga aatcagcgag acgagttaca
    24901 tcaacaaact ccgacgcctt gctcaacggg agccggactt tattgatata cacgctcatc
    24961 tggcatatgc atttctggaa cagaatgccc cgcgtaaggc actgaacgcc gctcttaaag
    25021 gactggcggc aggtaatcgt atcatcccgg aaagtttctg tggtgaaata atctggatgc
    25081 atcccgaaaa ccggccttat ctgcgcgcgc tttatgcagc aattctcgca aatgtgcact
    25141 tacagcgcca tcaggatgcg gttatgctca ctgataaaat actggcatac aatcctgaaa
    25201 ataatcaggg ggctcgctgg ttgttgggtt cagagctgtt acgcaccggc gatcatgagc
    25261 gagccttcag tgtactgaaa gagcatgctg atgaattttc accttactgg tatgaactcg
    25321 gtctcctgca ttttctgaac ggggaacatg ttaaggccgc cacagctttt cgccatggct
    25381 ttgccacaaa cacttacatc gcagaaatgc tgtgcggaaa tctgcatcca tttccacttg
    25441 ctgtgtggca tgacttctcc ggctctctgg atacggcaga agattactat gcaacatact
    25501 caccattatg gggacaatat ccggaagcat tgctgtttgt aaactggctc tataaccatt
    25561 cctccgtgct gcatgaacga tcagaaatca ttaaatgcgc cgaaatgctg attcaggaag
    25621 atgatttcga aatatgcgaa agtattttaa gacagcagga gcacctgtgg aaaagaattg
    25681 acaagacact ttctgaggaa attgtacaga aatgcagaaa tatgaacggt gaatatatct
    25741 ggccctggat attgcccttt tccgcagcag ggatgaaaca ttcaagcatc cagtatcagt
    25801 agatattgca ttagtgtaac tttaaaacaa gtaataattt attatccaat aataacactg
    25861 tgttccttcc ccaggtgatt accctggggt tattttctga aagtttcctt tgcaaaagga
    25921 tagcgttgta aattacttta acgaattact ccatgaactc attaaagttc tttcgtaata
    25981 ctttatcgtc aaacacgtta tagctggcat caaaaaccac agatttaccc tgccagcggg
    26041 taatcccctc ctctggcgtt ttcggttgaa ctggtatccc cgtccaacac aggcagccat
    26101 tataaacagt aaaaaattgt attcgctgtt atgaaatcaa tagtcctgac gtactgttga
    26161 atgtgcttgt gttctgaacc tcgtacaacc agtacatttg atatcgaatt ataaacaaaa
    26221 cattgcatcc aactttttta atatggacaa tagttgtcca tacggtggtt ttatattcga
    26281 taaatcaatg gatttcattg agtcacagga ctggtaatgg atgaaaatgc tttaaggttt
    26341 gcctcatact ggcgcaactc gcttgcagat gctgagtcag gaaagggctg ttttgaacgg
    26401 aaagacgccc aaaatttcac tcactggcat gggatagcgg cgggacgtct tgacgaagcg
    26461 attgtcaata aattttttaa gggagaaaaa gacgatgtcg aaactgtcaa tgtcatctta
    26521 cgcccaaaag tttatttccg gttactgcag catggtaagg accgttccgc aggcacgcct
    26581 gatattgtta caccgatagt gacgccagcc ttgctaagcc gtgagggttt tttatatccg
    26641 acgccagcga cctccattcc cagagacctg cttgaacctt tgccaaaagg agcattttcg
    26701 attggtgaga ttgggcagta tgacaaatac aagacgaccc atactgatcc tacccacgta
    26761 atgtggacac ggccctaagc gagattatgg ttttcaaatt gttccgggct gagaccgcca
    26821 caggcactgt gacgacgcca gcgattgtaa tcacactcga tataattaaa caccgttgcc
    26881 cgcattattt cccggctgct aaagcgttcc ccgtggatac attccacctt cagcgaatga
    26941 aagaagcttt ccacacaggc attgtcataa cagtagcctt tcgcactcat actgccacgc
    27001 aggttgtgtc gcttcagcat tgcctgataa tcccctgaac agtattgacc accacggtcc
    27061 gtatgaacaa tgacgctttc cgggcgtctt ctccgccaca acgccatttg cagtgcatca
    27121 caggccagtt gtgctgtcat tcgcggtgac atcgaccagc caatgacgga gcgtgaccac
    27181 aggtcgatga ctactgcgag atacaaccag ccctcatcgg tacgcaagta cgtgatgtca
    27241 cccgcccact tctggtttcg ggccactggc gctgaagtcc tgctccagca gattctccaa
    27301 tacgggcagg ccatgtgcac ggtagctgac cgggctgaac ttccggctgg ctttcgcccg
    27361 cagcccctga cgacgcaggc tggcggcaat ggttttaata ttgaactccg gcagttcgtc
    27421 agcaaggcgg ggagcaccgt atcgctgctt tgcctcaaag aatgccttat ggacagcggc
    27481 atcgcaggtg agccgaaact gttggcgcag gctcatctgg tgacgacgcc tgagccagac
    27541 ataccagccg ctgcgggcaa ctcgaagtac acgacacatc gctttgatgc tgaactctgc
    27601 ccgatgattt tcgataaaga catacttcat ttcaggcgct tcgcgaagta tgtcgcggcc
    27661 ttttggagga tggacagttc ctcagcctgc tccgccagtt gtcgtttaag gcggacattt
    27721 tcagcggcca gttcgctttc gcgctctgac gaactcattt gttgctgctg tttactgcgc
    27781 caggcataaa gctgagattc atacaggctg agttcacggg ctgcggcggc cacaccgatg
    27841 cgttcagcga gtttcagggc ttcgttacga aattcaggcg tatgttgttt acggggcttc
    27901 ttgctgattg atactggttt tgtcatgagt cacctctggt tgagagttta ctcacttagt
    27961 cctgtgtcca ctattggtgg gtaagatcac cctccattaa aactaaaacg ggcgctttct
    28021 gatatatcat ctgtaagcgc agtaatacca ttagtagcac ctctcggcgg cgcattgatt
    28081 attgaatatt ataactggaa tgttatattt cttgttatga tgctgttcag tattataata
    28141 atgttattat caccaaagat aactcatgca aatgagaaag taatatgtca aaacagtgca
    28201 tcctttgtga ccaaagagtt tatctgcggc actatacttg tctcattaat actcagttct
    28261 ctattttgct acatatcaat atcaccatca attttcatgc atacttatgg cctgagtact
    28321 tttagttact caattatttt ctccttctca gttttatttt tcataatcgg aaatcaatta
    28381 tcaaagattg aaaaattatc caacctatgg tttttattac ccaatagcca attttatcgc
    28441 attacaaata aatggttcaa aaacaggatt tgccgcatct attacaggtt ttactcaaac
    28501 aataagcgct ggtacaatca gtttcctgtc agtaaccgta agcgacaacg gtgttggtcc
    28561 cggaactact ttaggtcttg gttcactaat aacttcatta ctgtcaatag ccgtaatcgc
    28621 cattgcagga aataataaaa agtaagtgta ctctgactgc gtatgtatct acttgaaccg
    28681 ccccgg
//
LOCUS       DBKELA010000050        28419 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.050, whole genome shotgun sequence.
ACCESSION   DBKELA010000050 DBKELA010000000
VERSION     DBKELA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.050"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            564..1334
                     /locus_tag="ACUQR0_002120"
     CDS             564..1334
                     /locus_tag="ACUQR0_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine ThrE exporter family protein"
                     /protein_id="HHV9109150.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="ACUQR0_002121"
     CDS             1325..1798
                     /locus_tag="ACUQR0_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HHV9109151.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="ACUQR0_002122"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="ACUQR0_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HHV9109152.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="ACUQR0_002123"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="ACUQR0_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HHV9109153.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /gene="yjjA"
                     /locus_tag="ACUQR0_002124"
     CDS             3233..3727
                     /gene="yjjA"
                     /locus_tag="ACUQR0_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HHV9109154.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="ACUQR0_002125"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="ACUQR0_002125"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HHV9109155.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="ACUQR0_002126"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="ACUQR0_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="ACUQR0_002127"
     CDS             7571..8485
                     /locus_tag="ACUQR0_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HHV9109156.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="ACUQR0_002128"
     CDS             8700..10061
                     /locus_tag="ACUQR0_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109157.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="ACUQR0_002129"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="ACUQR0_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HHV9109158.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="ACUQR0_002130"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="ACUQR0_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HHV9109159.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /gene="hpaG"
                     /locus_tag="ACUQR0_002131"
     CDS             12611..13900
                     /gene="hpaG"
                     /locus_tag="ACUQR0_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HHV9109160.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="ACUQR0_002132"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="ACUQR0_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HHV9109161.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16204
                     /gene="hpaD"
                     /locus_tag="ACUQR0_002133"
     CDS             15365..16204
                     /gene="hpaD"
                     /locus_tag="ACUQR0_002133"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HHV9109162.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRTI
                     LKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWREGQFK
                     EFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVNAVFP
                     LPA"
     gene            16214..16594
                     /locus_tag="ACUQR0_002134"
     CDS             16214..16594
                     /locus_tag="ACUQR0_002134"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HHV9109163.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16662..17465
                     /gene="hpaH"
                     /locus_tag="ACUQR0_002135"
     CDS             16662..17465
                     /gene="hpaH"
                     /locus_tag="ACUQR0_002135"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HHV9109164.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17476..18279
                     /gene="hpaI"
                     /locus_tag="ACUQR0_002136"
     CDS             17476..18279
                     /gene="hpaI"
                     /locus_tag="ACUQR0_002136"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HHV9109165.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18301..19656
                     /gene="hpaX"
                     /locus_tag="ACUQR0_002137"
     CDS             18301..19656
                     /gene="hpaX"
                     /locus_tag="ACUQR0_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HHV9109166.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19666..20556
                     /gene="hpaA"
                     /locus_tag="ACUQR0_002138"
     CDS             19666..20556
                     /gene="hpaA"
                     /locus_tag="ACUQR0_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HHV9109167.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20664..21520
                     /locus_tag="ACUQR0_002139"
                     /pseudo
     CDS             20664..21520
                     /locus_tag="ACUQR0_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21747..23305
                     /gene="hpaB"
                     /locus_tag="ACUQR0_002140"
                     /pseudo
     CDS             21747..23305
                     /gene="hpaB"
                     /locus_tag="ACUQR0_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23323..23835
                     /locus_tag="ACUQR0_002141"
     CDS             23323..23835
                     /locus_tag="ACUQR0_002141"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HHV9109168.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24228..26378
                     /gene="btsT"
                     /locus_tag="ACUQR0_002142"
     CDS             24228..26378
                     /gene="btsT"
                     /locus_tag="ACUQR0_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HHV9109169.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26428..26631
                     /locus_tag="ACUQR0_002143"
     CDS             26428..26631
                     /locus_tag="ACUQR0_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HHV9109170.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26642..27598
                     /gene="yjiA"
                     /locus_tag="ACUQR0_002144"
     CDS             26642..27598
                     /gene="yjiA"
                     /locus_tag="ACUQR0_002144"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HHV9109171.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27667..>28419)
                     /locus_tag="ACUQR0_002145"
     CDS             complement(27667..>28419)
                     /locus_tag="ACUQR0_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HHV9109172.1"
                     /translation="TKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTED
                     FNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHA
                     DNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQAL
                     VNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYL
                     TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6957 a         7431 c         7120 g         6911 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tagcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcac
    15841 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    15901 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    15961 gttcgaccgc cagatggacg agcgtgtggt gaagctgtgg cgcgaaggcc agttcaaaga
    16021 gttttgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16081 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16141 taccgagctg ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgctgcccgc
    16201 gtaaggacgt tttatgccgc actttatcgt ggaatgtact gaaaatattc gtgaagaagc
    16261 ccgcttacct gagctgtttg ccagtgttaa cactgcactg gcggcaacgg ggatttttcc
    16321 gcttggggga atacgtagcc gggcgcactg gatcgatacc tggcaaatgg ctgatggcaa
    16381 gcacgattac gcctttgtgc atatgacgct aaaaatcggc agcggacgca gtctggaaag
    16441 ccgtcaggaa gtgggcgaaa tgctgtttga tctgattaaa acgcacttcg cctcactgat
    16501 ggagagccgt tacctagcgc tttcatttga gattgcagag ctgcacccga cgttgaattt
    16561 caaacagaac aacgtacacg cgttgtttaa ataaatttgc accagcttcc aggccggatg
    16621 ataaggcgct gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacacaccc
    16681 acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga
    16741 tctcgctgga ttacccggag atcaccatcg aagacgccta cgccgtgcag cgcgaatggg
    16801 ttcgcctgaa aatcgccgaa agtcgcacgc tgaaaggcca caaaatcggt ctgacctcga
    16861 aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggtgcactg atggacgaca
    16921 tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag
    16981 tggagctggc ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg
    17041 tttacaacgc cacggactat gtgatcccgg cgctggagct gatcgacgct cgctgccaca
    17101 acatcgatcc ggaaacccag cgcccgcgca aagtattcga caccatttcc gataacgccg
    17161 ccaacgccgg agtgatcctc ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt
    17221 ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg
    17281 tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg
    17341 tacaactgga agccgggcaa atcattctcg gtggttcgtt cacccgcccg gttccggcgc
    17401 gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg
    17461 tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgtccgcag
    17521 attggattat ggctggggct gagcagcagc tacagcgcgg agttactggc cggaccagga
    17581 ttcgactggt tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc
    17641 caactacagg cgattgcgcc ctaccccagc cagccagtgg tacgcccatc gtggaacgat
    17701 ccggtgcaaa tcaaacaact gctggacgta ggcacacaaa ccttactggt gccgatggta
    17761 caaaacgccg acgaagcccg taaagcggta cgcgccaccc gttatccccc cgccagtatt
    17821 cgcggtgtgg gcagtgcgct ggctcgcgcc tcgcgctgga atcgcattcc tgattacctg
    17881 caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag
    17941 aacttaccgc agattctgga cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat
    18001 ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt
    18061 gagcaggcga tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat
    18121 gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac
    18181 accaccctgc tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtaa tagttcttct
    18241 atttcgatgg cgaagcaaaa caacaactct gtctactaaa cgtacgatgt ggagcgcatc
    18301 atgacgacct ctaccctgca atataaagat aataaaactg ttgaaattga acatcgtgtc
    18361 attaataaat tatttcgtcg cctaatcatt tttcttttta ttctgtttgt tttttcgttt
    18421 cttgatcgca tcaatatagg atttgccgga ctgacaatgg gcaaggatct tggcctgacg
    18481 tccacaatgt ttggcctcgc cgcaacactc ttttatgtca cgtacgtcat gtgcgggatc
    18541 cccagtaata tgatgttagc gaaaattggc gctcgtcgat ggatcgctgg gatcatggtg
    18601 gtctggggaa ttgcttccac ctgcacaatg tttgcaacaa gccccgaaac actctatcta
    18661 ctgcgtatgc tggttggtat tgctgaggcg ggttttttac ctggtatttt ggtttatctg
    18721 acctggtggt tcccggcata tcatcgggcc agagccaatg ccttatttat gatagcgatg
    18781 ccagtcacta tgatgctggg attgatcctt tcaggctaca ttctggcctt ggatggactg
    18841 tggaatctaa aaggctggca atggctcttt ttgctggaag gtctgccatc ggtcattctt
    18901 ggtgtcgtca cctggtttta tctcaacgac acaccagacc aggcgacctg gcttgatgaa
    18961 aacgaaaaac aggctttaaa aacgatgatt ctccgtgaac aagagagtgc atttgcccat
    19021 gcatctgttc cacgctcaac cttacgtgaa gtcctgaccc cagccatcat gctatataca
    19081 cttgcttatt tttgcctgac taatactctg agtgcgatta atatctggac gccgcaaatc
    19141 ctacagagtt tcaatactgg cagtagcaac atcatgattg gtttgctggc ggccatcccc
    19201 caattctgca caatttttgg catgatctgg tggagtcgtc gttccgaccg tcttaaagaa
    19261 cgcaaaaagc atacgattct tccctacctg tttgccgctg ccggatggtt actcgcttct
    19321 gcgacagatc acagcctcat tcaactgagc agcatcatta tggcctcaat gggatcattt
    19381 accgcgatga ctatcttttg gacaacaccg gatcaagtta taagcttgca atcgcgtgcc
    19441 gttgcgatgg ctgtcatcaa tgctgtcggc aatgtaggtt cagcagtcag tccattactt
    19501 atcggtattt tgaaagatgc aaccggcagt tttagctcag gtttgtggtt tgtagcagga
    19561 ttgctggtac tgggcgcgtt agtcatgacc cgaatcccta tggggagtcg ggaacactct
    19621 ctttcatctg aacttatcgc accaaaaccg caataaggag tcatgatgtc cagtgaagct
    19681 atctttgaac atggttatga aaatattgat ttggtccggg aatatgatgc ccgctacgct
    19741 ggtgaagatg tgcattatga aacatttgcc cggttagcga atttctttgg acgcgatatg
    19801 cgccctcact ggcacgatag atattttcaa ctccattatc tggtcactgg acgcattact
    19861 ttacaactgg atgagcactt ctattctgtg cgcgcaccgc tttttgtact aacaccacca
    19921 tcggtgccac atactttctt tactgatcct gatagcgatg ggcatgttct gaccgtacgt
    19981 caggagttaa tttggcccct ggtagaaaag ctctggcctg gtagtggaga tgccgcaatg
    20041 ctacaaggta tctgtctgtc actggaggga atggaacata cacaggcggc attaaatcat
    20101 tattggccga taatatccct ggagtttcaa caaaataagc aaggacggga aattctattg
    20161 caatcactgg cgcaaagtat ttttacaatc ctactgcgtg aagcaccacc aaatgatatt
    20221 tcaacatgca gcgtgcgcgg cgagatgtgt ttattccaga agtttaatcg gctgattgac
    20281 gaaaaatatc gccaacattt aatggtccct gaatatgcaa caatgctcgg tatgagtgaa
    20341 tcacggctta ccgagctgtg ccgacgtttt gccaatcaat cgccgaagag attgattttc
    20401 gaacgagtga gccgggaagc cagacggctt ctgctctata gcgagcaaag catcaaccag
    20461 atagcccttg atcttggcta caaagatccc gcctatttcg cccgtttttt caaccgaatg
    20521 atgggttgcc caccaacaca attccgtggt cgctgaccat ttatcaacct gagagactcg
    20581 acatgaaaat atctttacgc tcactatttg tggcgtgcct gattgcgcca gtggcattca
    20641 cctacgcctc cgaaccgcaa taaatgaaaa ttgtactact tggaacgaaa ggtggccctt
    20701 ctttattaaa agctgaccgt ttaccgatgg caacggcact tattattggc aataaaacct
    20761 agttaattga tgctggttac ggggccagct tacaactggt aaaaaataat attccactgc
    20821 gccagattga taatatttta ctcacccatt tacacagtga tcatattctg gactatccat
    20881 cagtcttaat gaatgcatgg gctagcggtc tgcaggacaa aactattcat gtattcggtc
    20941 cgccaggcac ccgagccatg accgctgcca gttggcaggt cttcgatcgg gacatcacac
    21001 tgcgtatgga agaagaagga aaacccgatc ctcgcaaact cgttacagct caagatgttg
    21061 gtgaaggcgt agtttataaa gatgataccg tgaccgtgac tgcattaaag gtccctcatt
    21121 ctcctttccc tgacggtgag gcttttgctt atcgcttcga cacacaaggc aagcgaattg
    21181 tcttctcagg agatacctcg tactttcccc cactggcaac tttctcagaa ggcgctgata
    21241 tacttgtgca tgaggccgta catgagccct ccgtcgctaa gctggcagac agtattggca
    21301 atggtaaaac actggcaaaa gccattgctt ctcaccatac gactattgag gatgtaggca
    21361 aaattgccaa tgaagcaaaa gtgaaaaaac tcatccttag ccacctggta ccaatcaccg
    21421 ttccggatga aacatggaag caagaagcac agaaaactat tcaggcccta tcgtcgttgg
    21481 tcatgaaaat atgacgatta ccattccatt cgccccataa aaaattccgt ccccgttagc
    21541 ccttgttagc ggggaatatt atgatttgcc ttactgtaag ttgatcacga tcgcagttta
    21601 ctatttaact ccaccttgct gtcgaaaagt accaacgttc gcctgaaacg tctcttcccg
    21661 acaaccgcgt tcaacgcttc aattaaataa caaaaacaac acacaaaatt aacaatcgta
    21721 ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac
    21781 gtcctttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct
    21841 atggcgagcg agtgaaagac gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg
    21901 ttgcccaact gtacgacgcg ctgcacaaac cggagatgca ggactctctg tgttggaaca
    21961 ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg
    22021 acctgcgcca gcaacgcgac gccatcgctg aatggtcacg cctgagctat ggctggatgg
    22081 gccgtacccc agactacaaa gccgctttcg gttgcgcact gggcgcgaat ccgggctttt
    22141 acggtcagtt cgagcagaac gcccgtaact ggtatacccg tattcaggaa actggcctct
    22201 actttaacca cgcgattgtt aacccaccga tcgtcatttg ccgaccgata aagtgaaaga
    22261 cgtttacatc aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt
    22321 ggttgccacc aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt
    22381 gatgggcgaa aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt
    22441 gaaattaatc tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga
    22501 ctacccgctc tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtact
    22561 gatcccatgg gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc gctggacgat
    22621 ggaaggcggt ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact
    22681 cgacttcatt acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg
    22741 tggtgtgcag gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag
    22801 tgactcgatg tgttcagaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc
    22861 cgcactgcaa acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat
    22921 agaacgcaac gttaccagtg gcctgatcta cctcccttcc agtgcccgtg acctgaacaa
    22981 tccgcagatc gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt
    23041 ccagcgcatc aagatcctca aactgatgtg ggacgccatt ggcagcgaat ttggtggtcg
    23101 tcacgagctg tatgaaatca actactccgg tagccaggat gagattcgcc tgcaatgtct
    23161 gcgccaggca caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct
    23221 gtcggaatac gaccagaacg gctggactgt gccgcacctg cacaacaacg acgatatcaa
    23281 catgctggat aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa
    23341 cgcctgcgct ttcatgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc
    23401 gagggcgacg ccggacaatg cgggattacg gcaacggctg tctgctcggt cacggataca
    23461 ccaccgtcgc tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc
    23521 aacggcaagt tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc gcgccacttc
    23581 gcgggcatga caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt
    23641 ccgctggcgc agccggtgct aaaaggttcg ctggccagtc ttgaaggaga gatccgcgat
    23701 gtgcaggcga ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt
    23761 gcagaaggtc acggacttat ttactttaaa cgccgtttcc atccggtgat gctggaaatg
    23821 gaagctgcga tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg
    23881 atctcaatca catatctacc cgatgaataa ttcttgccca accgctcacc gccattcctg
    23941 accgcttaca tccctaaaat aaccactcag ttatttacct tactttacgc gcacgtatct
    24001 ctggcaacat cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag
    24061 aacggcttcg gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc
    24121 aagtgagtga atccccggga gcttacaaaa gtaagtgtca gaggtgaacg aacgcagctg
    24181 ccgcacctgt aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa
    24241 aactattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
    24301 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt
    24361 cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac
    24421 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
    24481 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg
    24541 tgggaccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg
    24601 gggtagtgct ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta
    24661 acggcgcatc tctcggtgag atgatcaaag aagagatggg gccagtaccg gggactatcg
    24721 cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg
    24781 ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg
    24841 cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg
    24901 ttattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
    24961 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
    25021 atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg
    25081 caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc
    25141 cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag
    25201 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
    25261 cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca
    25321 ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt
    25381 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgccggc cttggcatca
    25441 ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga
    25501 aagacgttac cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag
    25561 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
    25621 gtgcgccgac gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg
    25681 acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc
    25741 tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
    25801 cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg
    25861 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
    25921 tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca
    25981 ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg
    26041 tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc
    26101 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
    26161 acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
    26221 caggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
    26281 cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa
    26341 tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc
    26401 ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat
    26461 ctcggccagg cggcgaagat gttgattggt attccagact acgacaacta cgtcgagcat
    26521 atgaagacca accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc
    26581 cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag
    26641 catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca aaactaccct
    26701 gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt
    26761 cggcgaagtc tccgttgatg atcaactgat tggcgaccgc gccacgcaga tcaaaacgct
    26821 gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaggacgcac tactcgacct
    26881 gctggataat ctcgacaagg gcaatattca gttcgatcgt ctggtgattg aatgcaccgg
    26941 catggccgat cccggcccga ttattcagac ctttttctcc catgagattt tatgccagct
    27001 ctacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa
    27061 ccagttcacc atcgcccagt cccaggtggg ctacgccgac cgtattctgc tgactaaaac
    27121 cgacgtcgca ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc
    27181 accggtctac accgtcaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg
    27241 ttttatgctg gaagagaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa
    27301 acaaaacgat atttcgtcaa ttgtggtgga actggattac ccggtagata tcagcgaagt
    27361 ttcccgcgtg atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg
    27421 gatgctgtgg attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta
    27481 cagcgccgac tgggacaggc cgtggggcga tgaaaaaccg catagcacaa tggtgtttat
    27541 cgggattcag ttgccggagg aagagattcg ggcggcgttt gcgggattga ggaagtaagt
    27601 tcgggaaaga tcaacttttt gagagttcca ccgcaacatt attacgagtg gaactcttta
    27661 gatatgtcag accaaccaaa gcttcttcaa ttgcgttttc attttttcat gtctcatttt
    27721 cagttcagcc aatccccatt ctgaagaatt acaaacatct tttgttaaat aaaaagagct
    27781 tttactatta agtttctcat aaatagattt ctttttgata aaatcatagt gttgtgtttc
    27841 agaatttttg gaaccactta tgagtgttaa attacctatt ttatgcagcc agtagacatg
    27901 ctcttgaggt tggaatctat taatccagta ttcattaact aatgcttgag gaagaatatg
    27961 ctcaattgtg attctccccg tataggtttt aatcacgctc tcatcttgtt gctccatatc
    28021 taagcgaagc aaaattgctt ttattaagtt cacttgattc ggacgaggct catataaatc
    28081 ttcatccaga gcagctataa aacctgaatt atctgcatgt tggtttatct gatttatcac
    28141 agaatcaaaa ggcatatcat tatttattgc taccagagca gaataacaaa ccatttctct
    28201 ttggctttta atttgttttt ttaaccaccc atgcatatat accttctcaa aggcggtaat
    28261 aaattgagaa aaatcatcta aattaaaatc ttctgtacga gccattcgat tcatgaatgc
    28321 cataaccgga ggaatccact catctacccc taaattactt agcgatgcta tttttcgtcg
    28381 tattgaaggg tgttcaaaat cattctctaa aatcttagt
//
LOCUS       DBKELA010000051        28419 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.051, whole genome shotgun sequence.
ACCESSION   DBKELA010000051 DBKELA010000000
VERSION     DBKELA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.051"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..156)
                     /locus_tag="ACUQR0_002146"
     CDS             complement(<1..156)
                     /locus_tag="ACUQR0_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HHV9109173.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKSCRLAP"
     gene            337..1968
                     /locus_tag="ACUQR0_002147"
     CDS             337..1968
                     /locus_tag="ACUQR0_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHV9109174.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1965..2684
                     /gene="dcuR"
                     /locus_tag="ACUQR0_002148"
     CDS             1965..2684
                     /gene="dcuR"
                     /locus_tag="ACUQR0_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HHV9109175.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3255..4595
                     /gene="dcuB"
                     /locus_tag="ACUQR0_002149"
     CDS             3255..4595
                     /gene="dcuB"
                     /locus_tag="ACUQR0_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HHV9109176.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4673..6319
                     /gene="fumB"
                     /locus_tag="ACUQR0_002150"
     CDS             4673..6319
                     /gene="fumB"
                     /locus_tag="ACUQR0_002150"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HHV9109177.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQIAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6442..7071
                     /locus_tag="ACUQR0_002151"
     CDS             6442..7071
                     /locus_tag="ACUQR0_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HHV9109178.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7210..8631)
                     /gene="melB"
                     /locus_tag="ACUQR0_002152"
     CDS             complement(7210..8631)
                     /gene="melB"
                     /locus_tag="ACUQR0_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HHV9109179.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8746..10089)
                     /gene="melA"
                     /locus_tag="ACUQR0_002153"
     CDS             complement(8746..10089)
                     /gene="melA"
                     /locus_tag="ACUQR0_002153"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HHV9109180.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10372..11280
                     /gene="melR"
                     /locus_tag="ACUQR0_002154"
     CDS             10372..11280
                     /gene="melR"
                     /locus_tag="ACUQR0_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HHV9109181.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11479..13746
                     /gene="adiA"
                     /locus_tag="ACUQR0_002155"
     CDS             11479..13746
                     /gene="adiA"
                     /locus_tag="ACUQR0_002155"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HHV9109182.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14071..14832
                     /gene="adiY"
                     /locus_tag="ACUQR0_002156"
     CDS             14071..14832
                     /gene="adiY"
                     /locus_tag="ACUQR0_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HHV9109183.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14969..16306
                     /gene="adiC"
                     /locus_tag="ACUQR0_002157"
     CDS             14969..16306
                     /gene="adiC"
                     /locus_tag="ACUQR0_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HHV9109184.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16410..18053
                     /gene="eptA"
                     /locus_tag="ACUQR0_002158"
     CDS             16410..18053
                     /gene="eptA"
                     /locus_tag="ACUQR0_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HHV9109185.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18050..18718
                     /gene="basR"
                     /locus_tag="ACUQR0_002159"
     CDS             18050..18718
                     /gene="basR"
                     /locus_tag="ACUQR0_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HHV9109186.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18719..19819
                     /gene="pmrB"
                     /locus_tag="ACUQR0_002160"
     CDS             18719..19819
                     /gene="pmrB"
                     /locus_tag="ACUQR0_002160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HHV9109187.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19795..19884)
                     /gene="pmrR"
                     /locus_tag="ACUQR0_002161"
     CDS             complement(19795..19884)
                     /gene="pmrR"
                     /locus_tag="ACUQR0_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HHV9109188.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19996..21498)
                     /gene="proP"
                     /locus_tag="ACUQR0_002162"
     CDS             complement(19996..21498)
                     /gene="proP"
                     /locus_tag="ACUQR0_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HHV9109189.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21762..22640)
                     /locus_tag="ACUQR0_002163"
     CDS             complement(21762..22640)
                     /locus_tag="ACUQR0_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HHV9109190.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22637..24865)
                     /gene="crfC"
                     /locus_tag="ACUQR0_002164"
     CDS             complement(22637..24865)
                     /gene="crfC"
                     /locus_tag="ACUQR0_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HHV9109191.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25267..25602
                     /locus_tag="ACUQR0_002165"
     CDS             25267..25602
                     /locus_tag="ACUQR0_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HHV9109192.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25760..26203
                     /gene="yjdN"
                     /locus_tag="ACUQR0_002166"
     CDS             25760..26203
                     /gene="yjdN"
                     /locus_tag="ACUQR0_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HHV9109193.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26336..27124
                     /gene="phnC"
                     /locus_tag="ACUQR0_002167"
     CDS             26336..27124
                     /gene="phnC"
                     /locus_tag="ACUQR0_002167"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HHV9109194.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27149..28165
                     /gene="phnD"
                     /locus_tag="ACUQR0_002168"
     CDS             27149..28165
                     /gene="phnD"
                     /locus_tag="ACUQR0_002168"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9109195.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28271..>28419
                     /locus_tag="ACUQR0_002169"
     CDS             28271..>28419
                     /locus_tag="ACUQR0_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HHV9109196.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7299 a         7426 c         6940 g         6754 t
ORIGIN      
        1 gggggccagt ctacacgact ttcgtttgag attaaataac ttgccgttgc cacgtacgca
       61 attgccagaa tgctgacata tcgcagtgtt gaaatagaca tcgatctttt cgccggtata
      121 acagcgataa cccccgtcca gtagcgcctg atccatgacc ttccctctct catcagtatg
      181 attatcgcta taagcatagc cccggaatct tcatacagca ctggcagttt ccgttgccag
      241 agtctcttcc cctatatagc caatcatttt tactcccttt gaattacccg cctcatcaga
      301 gataatgctt aagaaatttg tcacacaagg aagctgatga gacattcatt gccctaccac
      361 atattacgca aacgtccgat gaaattgagt accactgtga tcttaatggt cagcgcggta
      421 ctgttctcgg tgctattggt ggtgcatctg atttacttct cgcaaatcag tgatatgacg
      481 cgagacgggc tggccaacaa ggcactggca gtggcgcgta ccctcgccga ctcgccggaa
      541 atccgtcagg gcttgcagaa aaaaccgcag gagagtggca tccaggccat cgcggaagcc
      601 gtgcgcaaac gcaacgatct gctgtttatt gtcgttaccg atatgcaaag tcttcgctac
      661 tcacatcctg aagcccagcg tattggtcag ccatttaaag gtgatgacat ccttaatgcg
      721 ctgaatggcg aagaaaatgt cgctatcaat cgcggttttc tggcgcaggc tttacgcgta
      781 tttaccccca tctacgatga aaatcataaa caaattggcg tggtggcgat cggccttgag
      841 ttaagccgcg tgacccaaca gatcaatgac agtcgctgga gcattatctg gtcggtatta
      901 tttggcatgc tggtcggact gattggcacc tgcattctgg ttaaggtact gaaaaaaatc
      961 cttttcggcc tggaacccta cgaaatctcc acgctgtttg agcaacgcca ggccatgttg
     1021 cagtctatca aagaaggcgt cgttgccgtg gacgatcgcg gcgaggtcac gctgatcaac
     1081 gatgccgcac aagaattgct gaattaccgt aagtcgcagg acgatgagaa actgtcgacg
     1141 ctcagccact catggtcaca ggtggtagat gtctcggaag tgttacgcga cggtacgccg
     1201 cgccgcgacg aagagattac gattaaagac cggctattac tgatcaacac cgttccggtg
     1261 cgcagtaatg gcgttatcat cggtgccatt tcaaccttca gggacaaaac tgaagttcgt
     1321 aaactgatgc agcgactcga cggtctggtc aactatgctg acgcacttcg tgaacgatcc
     1381 cacgaattta tgaataaatt gcatgtgatt ctcggattat tgcatctgaa gagttataag
     1441 cagttggaag attacattct caaaacagcc aataactatc aggaagagat tggctctctg
     1501 ctgggtaaga tcaaatctcc ggttatcgct ggttttttaa tcagcaagat taaccgcgcg
     1561 accgatttag gccatacgct gattttaaac agtgaaagcc agctgccaga cagcggcagt
     1621 gaggaccagg tcgcgacgct gattaccaca ttgggaaatc tgatagaaaa cgcgctggaa
     1681 gcattagggc cggaacccgg tggcgaaatt agcgtaacat tgcactaccg tcacggctgg
     1741 ctgcactgcg aagttaacga tgatggaccg gggatcgcac ccgacaaaat cgatcacatt
     1801 tttgacaaag gtgtctcgac aaaaggaagc gagcgaggcg tcggtttagc acttgtcaaa
     1861 caacaggtag aaaatctcgg cggcagcatc gccgtggaat cggaaccggg gattttcaca
     1921 caattttttg tccagatacc ctgggacggg gagaggtcga acagatgatc aatgtattaa
     1981 ttatcgatga cgacgcaatg gtcgcggagc tgaatcgccg atacgtagca caaatcccag
     2041 gctttcaatg ctgtggaaca gcctcgacgc tggagaaagc caaagagatt atcttcaata
     2101 gcgatgcgcc tatcgacctg atattgctcg atatctatat gcaaaaagag aacgggctcg
     2161 atttactgcc tgtcctgcat aacgcgcgtt gcaaaagtga tgtgattgtc atctcctccg
     2221 cagccgatgc ggcaaccatt aaagattcgc tgcattacgg tgtcgtggat tacctgatca
     2281 aacccttcca ggcttcccgc tttgaagagg cgctcaccgg ctggcggcaa aagaaaatgg
     2341 cgctggaaaa acatcagtat tatgaccagg ccgagcttga tcagctaatc cacggcagca
     2401 gctccaacga acaggatcct cgtcgcttgc caaaaggctt aacgccgcag acgttacgca
     2461 cgctgtgcca gtggattgac gcgcatcagg actatgagtt ttcaaccgac gagctggcta
     2521 acgaggttaa catctcgcgt gtttcctgcc gaaaatacct catctggctg gtcaactgcc
     2581 acatcttgtt caccagtatc cattatggcg tcacggggcg tccggtttac cgttaccgta
     2641 ttcaggcaga acactactca ctgctgaaac aatattgcca ataacggatc gctggttatc
     2701 tgtaagtaat aattattacc gtaatgcaaa atacccctgg cacaagttac atcgttaatc
     2761 agtaaaactt gtgccagatc aaatataatt atccctccat catcgttaaa aattaatatc
     2821 tcttcagatg aacggtgttt ttaatttcaa aacgataaca aaagttaatt aactattatg
     2881 tcacccgcat tatgtgtatt tttacccaca aatgggtaga tcagattaat ctataaacct
     2941 aatgatatct gccctgagaa caaaaaatag accgataaat atcaataaga taacagcaaa
     3001 caaaacatta acatctgcgc agtacaaact ataaacccat cgccagagag tttttctctc
     3061 tgaaaaagcc gcttatcaca gtgcataaat ttgccgctgc tttaatcagc caatattcac
     3121 tgtgaggtat ttgctaaagc cggtaacgac caaacggata tttagtcagg ctctgaaaac
     3181 agttcataca aaacagaacg tgactgtgat ctattcagca aaaatttaaa taggattatc
     3241 gcgagggttc acacatgtta tttactatcc aacttatcat aatactaata tgtctgtttt
     3301 atggtgccag aaagggtggt atcgcgctgg gtttattagg cggtatcggt ctggtcattc
     3361 tggtcttcgt cttccacctt cagccaggta aaccaccagt tgatgtcatg ctggttatca
     3421 ttgcggtggt ggcggcatcg gcgaccttgc aagcttcggg cggtcttgat gtcatgctgc
     3481 aaattgccga gaagctgctg cgccgcaacc cgaaatatgt ctcaattgtc gcgccgtttg
     3541 tgacctgtac actgaccatt ctttgcggta cgggtcatgt ggtttacacc attctgccga
     3601 tcatctacga cgtcgccatt aagaacaaca tccgtccgga acgtccgatg gcggcaagtt
     3661 ctatcggtgc acagatgggg attatcgcca gtccggtgtc ggttgcggtc gtgtctctgg
     3721 ttgcgatgct gggtaatgtc acctttgatg gtcgccatct tgagttcctc gatctgctgg
     3781 caatcaccat tccatcgacg ttaatcggta tcctggcgat cggtatcttc agctggttcc
     3841 gcggtaaaga tctggataaa gacgaagagt tccagaaatt catctccgta ccggaaaacc
     3901 gtgagtatgt ttacggtgat accgcgacgc tgctggataa aaaactgccg aaaagcaact
     3961 ggctggcaat gtggattttc ctcggggcaa tcgctgtagt cgcccttctt ggtgctgatt
     4021 cggacctgcg tccatccttc ggcggcaaac cgctgtcgat ggtactggtt attcagatgt
     4081 ttatgctgct gaccggggcg ctgattatta tcctgaccaa aaccaatccc gcgtctatct
     4141 caaaaaacga agtcttccgt tccggtatga tcgccatcgt ggcggtgtac ggtatcgcat
     4201 ggatggcaga aaccatgttc ggtgcgcata tgtctgaaat tcagggcgta ctgggtgaaa
     4261 tggtgaaaga gtatccgtgg gcgtatgcca ttgttctgct gctggtttcc aagtttgtaa
     4321 actctcaggc tgcggcgctg gcggcgattg ttccggtcgc gctggcgatc ggcgttgatc
     4381 cggcatacat cgtggcttca gcaccggctt gctacggtta ttacatcctg ccgacttatc
     4441 cgagcgatct ggcagcgatt cagtttgacc gttccggcac cacccacatc ggtcgcttcg
     4501 tcatcaacca cagttttatt ctgccggggt tgattggtgt gagcgtatcg tgcgtcttcg
     4561 gctggatctt cgccgcgatg tacgggttct tataaatgca ctttgcgtgc cgcccgtgac
     4621 tacgcggcac gccattttcg aataacaaat acagagttac aggctggaag ctatgtcaaa
     4681 caaacccttt atctaccagg cacctttccc gatggggaaa gacaataccg aatactatct
     4741 actcacttcc gattacgtta gcgttgccga cttcgacggc gaaaccatcc tgaaagtgga
     4801 accagaagca ctgaccctgc tggcgcagca agcctttcac gacgcttctt tcatgctacg
     4861 tccggcacac cagaaacaga ttgcggctat tcttcacgat ccagaagcca gcgaaaacga
     4921 caagtacgtg gcactgcaat tcttaagaaa ctccgaaatc gccgccaaag gcgtgctgcc
     4981 gacctgccag gataccggca ccgcgatcat cgtcggtaaa aaaggccagc gcgtgtggac
     5041 cggtggcggt gatgaagaag cgctgtcgaa aggcgtctat aacacctata tcgaagataa
     5101 cctgcgctac tcgcaaaacg cggcgctgga catgtacaaa gaggtcaaca ccggcactaa
     5161 cctgcctgcg caaatcgacc tgtacgcggt agatggcgat gagtacaaat tcctttgcgt
     5221 tgcgaaaggc ggcggctctg ccaacaaaac gtatctctac caggaaacca aagccctgct
     5281 gactcccggc aaactgaaaa acttcctcgt cgagaaaatg cgtaccctcg gtactgcagc
     5341 ctgcccgccg taccatatcg cgtttgtgat tggcggtacg tctgcggaaa ccaacctgaa
     5401 aaccgtcaaa ttagcaagcg ctcactatta cgatgaactg ccgacggaag ggaacgaaca
     5461 tggtcaggcg ttccgcgatg tccagctgga acaggaactg ctggaagagg cccagaaact
     5521 cggtcttggc gcgcagtttg gcggtaaata cttcgcgcac gacattcgcg ttatccgtct
     5581 gccacgtcac ggcgcatcct gcccggtagg catgggcgtc tcctgctccg ctgaccgtaa
     5641 cattaaagcg aaaatcaacc gcgaaggtat ctggatcgaa aaactggaac acaatctagg
     5701 tcagtacatt ccacaagaac tgcgtcaggc cggtgaaggc gaagcggtga aggtcgacct
     5761 taaccgcccg atgaaagaga tcctcgccca gctttcgcaa tatccggttt ccactcgttt
     5821 gtcgctcact ggcaccatta ttgtgggtcg agatattgca cacgccaagc tgaaagagct
     5881 gattgacgcc ggtaaagaac ttccgcagta catcaaagat cacccgatct actacgcggg
     5941 tccggcgaaa acccctgccg gttatccatc aggttcactt ggcccaacca ccgcaggtcg
     6001 tatggactcc tatgtggatc tgctgcaatc ccacggcggc agcatgatca tgctggcgaa
     6061 aggcaaccgc agccagcagg ttaccgacgc gtgtcataaa cacggcggct tctacctcgg
     6121 tagcatcggc ggtccggcgg cggtactggc gcagcagagc atcaagcatc tggaatgcgt
     6181 cgcttatccg gagctgggta tggaagctat ctggaaaatc gaagtagaag atttcccggc
     6241 gtttatcctg gtcgatgaca aaggtaacga cttcttccag caaatcgtca acaaacagtg
     6301 cgcgaactgc actaagtaac ctcttcggcc cagcgcctga cagcatgctg ccaggtgatc
     6361 cccctgggcc acctcttttg cgattgtaat ttcatgcttg ctggtgaata gtcagtattt
     6421 tccctgactt gcgaactcac catgacccgc acactcaagc cgttaattct taacaccggc
     6481 gcactggcgc taacgttaat cctgatttat accggcattt cggcccatga caaactcacc
     6541 tggctgctgg aagtgacacc ggtgattatc gtcgtgccgc tactacttgc caccgccaga
     6601 cgttacccgt taacgctgct gctctatacg ctcatcttct ttcacgccat catcctgatg
     6661 gtaggcgggc agtacaccta cgcgaaagtc cccgttggtt ttgaggtgca ggaatggctg
     6721 gggttgagtc gtaatccgta tgacaagctg gggcactttt tccaggggct ggtgcctgca
     6781 ctggtggcac gagaaattct cgttcgcggg atgtacgtgc gcggacgtaa aatggtggcg
     6841 tttctggtct gttgcgtggc gctggcgata agcgcgatgt atgaattaat cgagtggtgg
     6901 gcggcactgg cgatgggcca gggagcggat gattttctcg gtacccaggg cgaccagtgg
     6961 gacacgcaat ccgatatgtt ctgcgcgctg cttggcgcat taacgacagt gatattgctc
     7021 gctcgctttc attgccgcca gctacggcgc tatggcttga taactggata acaacgtgca
     7081 ctttgttgat gccggatgcg gcgtgaattc cttatccggt ctacaaaatt tgcaaatcca
     7141 aagcattaca ttaagacgtg gcctgataaa catatcgcat caggccattt tcgttagcta
     7201 ttgtccacgt caggctttca catcactcac tgcaccgacc ggaatatcag catgaacagg
     7261 ctcgggcggt actttgcgat atttatccag cagatgaatc tggatcctgc gcagcgtgtc
     7321 gccattgagg cgatagaaac ggaagtagag aatcagcgtt accatgaaaa acagagtcgg
     7381 tagagcaatc ataataaact gcatacctaa tagggcttgc gtagactgtt caacattcgg
     7441 tacatagcca atcatcccta acacaaccgc aatgaaaaaa gccgcaaagg ctgaaccgcc
     7501 cttcaccacc atagtctgca cggagtaagc gatgctttca cagcgtacgt gcagtttata
     7561 ttcaccgtaa tcaacggtat ctgccaccat gatgacctgt aatacccaga aaagcgccgt
     7621 tcccacattc agcaaaatac ccgcaatcac aatgaggacg acgttgtgat agctcattaa
     7681 tgccattaac aggagaacac cacagcttaa caccggaaga atagatgctc cggcccataa
     7741 aatgcgtcgg gataatgatt taactaagcg tgggaagaat actaacgtca ccaggttagc
     7801 agctcccgca tacgacagat aataggggaa caaatccgca tcaccgataa catatgagaa
     7861 ataatagata gcaaatccgg taataatgtt gctggctaca ttataagcaa gagccatacc
     7921 caagaggcat gaaagctgat cgtttttata aataagcgca acgatggctt taagtgtcag
     7981 atggcttcct tcagcagacg attgattgtc tgacgaaaag acttcatgca cattgcgcag
     8041 agtgatgatg gttgaaacaa taaaaaaggc gatcaatacc agagtgaaca tctgaaagcc
     8101 aaatccccga tcgccaccgc cgacataatt aacaaatggt agcgtcacac ctgccgtaac
     8161 aaagcctgcc agactggcaa aaaaacgcgg ataaggaacc agttgttcgc gttcacgttt
     8221 atcgagcgtg atggttggaa ccagcgacca gaagggaata tccataatgg tgtaagtcat
     8281 gccccagagg atgtaggtca cgcaaacaaa gacaatctga gtagtacctt caaacagatg
     8341 cgcactaaag aggagaaata agattacaga gtttgccaac gtaccgatca ggatccaggg
     8401 tttgaactta ccccatcgcg atcgcgtagc atttacaatc catcccataa tcggatcgtt
     8461 aatagcatcc cagatcctcg ccaccagaaa caaagtaccg accaaaccca cagacagccc
     8521 gacgacatcg gtgtagtaat acatgaggta catatacaca atgccgatcg caaaatcctt
     8581 cccgaacgct ccaaatccat aactgagttt tgtagtcatt gaaatgctca tagggtatcg
     8641 ggtcgctgtt accaacgccc gccttctgtt attggtatcg tcagaggtgt gcgccggaat
     8701 aaatcccccg gcgcagtagc gtttagtcgc gttttaacgg tgcaactagc ttggcagcca
     8761 gtcgccgtgg gcggcaatca ggtcgtcaac aagagcatat atttcgtcaa tgcccagcac
     8821 ggcggcagta tgcgggtcca tcatcgcggc gtggtaaaca cggtcgcgat tttccgtaag
     8881 aatagcttcg gtcagcagcg tctgtacgtt gatgttggtt tgcatcaggg cggccagatg
     8941 cgaaggtagc gtaccgactt tggtcggctg aatgccatta gcatcaacca gacaggctac
     9001 ttccacgcaa catccttgtg gcaggttatc aatcaaacca tcgttacgga cgttgccgta
     9061 aatcacactc ggctcgccag tccagatagc gttcatgatt gtgctggcat attcccgtga
     9121 cggtttaata tcaatccggg aggcgttttt atactcctcc agctctttat gccagttcgc
     9181 cagctgctcg acgcagcgtt tcgggtactc atccagcggt actttataac gctcaatcaa
     9241 atcctcacga cctagcttaa taaaccacgg cgtatattcg gcaaaatgtt ctgacgattc
     9301 cgtgacgaag tagcccagct ttttgaacat ttcgtagcgc acaatattct ggcagcgagt
     9361 attaccatga atatttggct tcggtgcctg ccctgcttca taagccgcca gcagttccgg
     9421 gtagagattc acgtaactgc cgtcggcggt tttgcgctcc agctccaggt aaaacgccat
     9481 atggttgata cctgcgcaac ggtaacgcag cgtagccggg tcgatattga ggtcacgcgc
     9541 cagctcttcc gccgttccct gcaccgaatg gcacagcccg acctgtttga tatacggata
     9601 gcgggcatac atcgcccagg tattcatcgc cattgggtta acatagttga gcatggtggc
     9661 atcggggcag acttccgtca tgttctcgca aatttgccac agatgcggaa tggtacgtag
     9721 cgcgcgcata ataccgcccg gccccaacgt atcggcaatg gtttgttcca gaccatgccg
     9781 cttacagacc tcgaaatcag tcaccgtgca aggttcataa ccgccaatct gaaatgccac
     9841 cacgacaaaa tcggcatcct ctaaggcttc tttctgttgg gtgtggcagg tgattttgcc
     9901 gctggcccct gctgaatcca tcagcttacg caccacaata tgcgactctt ccaggcgggt
     9961 ggggtcaatg tccatcaggg caatatgcgc cgttttcagc gcctcgcgat ggaacacatc
    10021 accaagaata tttttaacga aaatcgtcga accagcgccg ataaatgtaa ttttgggtgc
    10081 agacatcatg cagatctcct ggcttgcttg aataacttca tcatggcagg cgaatctgca
    10141 gaaaacctcc ggcttccagg ttgagcattc ccataaactc agatcctgcg tgaagcagca
    10201 gtaaatctga gtttatggga ataaattacc tgaaaagtag agttttcggc ctcatgatgc
    10261 tcggacagtc ccctcgcccc tctgggaaga gggtgaaaac cgtgctccca ctcgcagtca
    10321 tcctccctca ctcctgccat aattctgata ttctaggaaa gagagccatc catgaataca
    10381 gatacgttta tgtgcagcag cgacgaaaaa cagacccgca gcccgctgtc gctgtattcg
    10441 gaataccagc ggatggagat tgagtttcgc gcaccgcata tcatgcccac cagccactgg
    10501 catggtcagg tcgaagtgaa tgtgcctttc gatggcgatg tggaatacct gatcaacaat
    10561 gaaaaagtga atatcaatca gggtcatatc acgctgttct gggcctgtac accgcaccaa
    10621 ctaacagata ccggaacctg tcagagcatg gcgattttta atctgccgat gcatctgttt
    10681 ctctcctggc cgctggataa agacctgatt aaccacgtca ctcacggcat ggtgatcaaa
    10741 tcacttgcca cacagcaact tagcccgttt gaagtacgcc gctggcagca ggaattaaac
    10801 agtccgaatg agcaaattcg ccagctcgcc attgatgaaa ttggcctgat gctcaagcga
    10861 tttagcctct ctagctggga accgattctg gtcaataaaa cctcgcgcac acacaaaaac
    10921 agcgtctcgc gccatgcgca attttatgtt agccagatgc tgggctttat tgccgaaaac
    10981 tatgatcagg cgctgaccat caacgatgtg gctgagcacg tcaaacttaa cgccaactat
    11041 gcaatgggga tattccagcg ggtcatgcaa ttgacgatga aacagtacat taccgcgatg
    11101 cgcatcaacc acgttcgcgc gttactgagc gataccgata aaagtattct cgatattgcc
    11161 ctgacggcag gctttcgttc aagtagtcgt ttttacagca cgttcggcaa atatgtcggc
    11221 atgtcgccgc aacaataccg caaacttagc caacagcgcc gccagacgtt tcccggctaa
    11281 agacgatatc agtatcagcc aaaaaaatag tttagccgtc gataataagc agtgaaaata
    11341 atttaaaata atcacataac gtattgttta taaaactaaa tatttcaaaa atgtttgttt
    11401 ttcacgcgct ttacagcccg aaaaggccgg aagatacttg cccgcaacga agattccttc
    11461 ataaccgggt aagcaatgat gaaagtatta attgttgaaa gcgagtttct ccatcaagac
    11521 acctgggtcg gtaacgccgt tgagcgtctg gcagatgctt taagccagca aaatgttacc
    11581 gtgattaaat ccacctcctt tgatgatggt tttgccattc tctcttcaaa cgaagccatt
    11641 gactgcctga tgttcagcta tcaaatggag catccggacg aacatcaaaa cgtcagacaa
    11701 ttgatcggta agcttcatga gcgccaacaa aacgtgccgg tcttcctgtt gggcgatcgg
    11761 gaaaaagccc tcgccgcaat ggaccgggat ctgctggagc ttgtcgatga attcgcctgg
    11821 attctggaag ataccgccga ctttatcgcc ggacgcgccg ttgccgcgat gacccgctac
    11881 cgccagcagc tgttgccgcc actgttcagc gcgctgatga aatatagtga catccatgaa
    11941 tattcctggg cagcgccagg ccaccagggc ggcgttggtt ttaccaaaac acccgccgga
    12001 cgtttctacc atgactacta tggtgaaaat ctgttccgca ccgacatggg catcgaacga
    12061 acttccctcg gttctttgct tgaccatact ggcgcatttg gcgaaagcga aaaatatgcc
    12121 gcacgcgtat ttggtgccga tcgctcctgg tcggtagtcg tcggtacttc cggctctaac
    12181 cgcaccatca tgcaggcttg catgaccgat aacgatgtcg tggtcgttga ccgtaactgc
    12241 cataaatcca tcgaacaagg tttgatgctg acaggcgcga aaccggtcta tatggtgcca
    12301 agccgcaacc gctacggcat tatcgggcca atctatccgc aggaaatgca acctgaaacc
    12361 ttgcaaaaga aaatcagtga aagcccgctg accaaagaca aagccgggca aaaaccgtct
    12421 tactgcgtgg taaccaactg cacctatgac ggcgtgtgtt ataacgctaa agaagcgcag
    12481 gatctgctgg aaaaaacctc cgatcgtctg cactttgacg aagcctggta cggctatgca
    12541 cgtttcaacc cgatctatgc cgatcactat gccatgcgcg gcgaacctgg cgatcacaac
    12601 ggtcctaccg ttttcgccac ccactccatc cacaaactgc tgaatgcgct gtcacaggct
    12661 tcttatattc atgtacgtga aggtcgtggg gcgattaact tctcccgctt caaccaggcc
    12721 tacatgatgc atgccaccac ctccccgctg tatgccatct gcgcatccaa cgacgtggcg
    12781 gtgtcgatga tggacggcaa cagcggcctg tcactgacac aggaagtgat tgacgaagcg
    12841 gttgatttcc gtcaggcgat ggcgcggcta tataaagagt tcaccgctga cggtagctgg
    12901 ttcttcaaac cgtggaacaa agaagtcgtc accgacccac aaaccggcaa aacctatgac
    12961 tttgctgacg caccaaccaa actgctgacc accgttcagg actgctgggt aatgcatccg
    13021 ggcgaaagct ggcacggctt caaagatatt ccggataact ggagtatgct cgacccaatt
    13081 aaagtcagca tccttgctcc gggaatgggt gaagatggtg aactggaaga aaccggtgtt
    13141 ccggcggcgc tggtcactgc ctggcttggt cgccacggca ttgtgcctac ccgcaccact
    13201 gacttccaaa ttatgttcct gttctctatg ggcgtaaccc gtgggaaatg ggggactctg
    13261 gttaacaccc tttgctcctt caaacaccac tatgacgcca acacaccgct ggcgcaggtg
    13321 atgccggaac ttgttgaaca atatcctgac acttacgcga acatggggat tcacgatctg
    13381 ggtgacacca tgtttgcctg gctgaaagaa aacaaccctg gcgcacggtt gaacgaagcc
    13441 tattccggcc tgccggtggc ggaaatcacc ccgcgtgaag cgtacaacgc gattgtcgac
    13501 aacaatgtcg aactggtatc cattgaaaat ctgccaggac gcatcgcggc aaactcagtt
    13561 atcccgtatc cgccaggaat cccgatgctg ctgtctggtg aaaacttcgg cgataaaaac
    13621 agtccgcaag taagttattt acgctcgctg caatcctggg accaccattt ccctggattt
    13681 gaacacgaaa ctgaagggac tgaaattatt gacggtattt accacgttat gtgcgtgaaa
    13741 gcgtaaccac tattccgctg aaggcgtaat tgtttaaata acattacgcc gcctggcctt
    13801 aggccttttg agtatggcaa cgttttcata aaaattgctg caaacaaaaa tgtcatactt
    13861 tttgcgcggc cccaccccgc gctttttttt gcctgttatt tatcctgtaa aaaaatatgt
    13921 acatgagaaa ttactataaa aatttgtaat attagtaaaa ctcgttattt ttatgtatgt
    13981 ttatattcat catgcaatta tataaccatt tcccggtatc gctttgcttt agcgagaact
    14041 ggtcttttat tcgcactcag gagtacatgt atgaggattt gcagcgacca accttgtatt
    14101 gttttattga ctgaaaaaga tgtctggata agggtgaatg ggaaagaacc tattagcctt
    14161 aaagctaacc atatggcgtt attaaattgt gaaaataata ttatcgacgt ctcctctctt
    14221 aacaacactt tggttgctca tattagtcac gacatcatca aagattacct ccggtttctg
    14281 aataaagatc tctcgcaaat accagtatgg caacgtagcg ctacgcccat actcaccctg
    14341 ccatgcctga cgccagacgt ctttcgcgtt gccgcgcaac acagcatgat gcccgcagaa
    14401 actgagtcag aaaaggaacg aacacgcgca ttattattca cggtgctatc ccgttttctc
    14461 gacagtaaaa aattcctttc attaatgatg tatatgttac gtaattgtgt aagtgacagc
    14521 gtttatcaaa ttattgaaag cgatattcac aaagactgga atcttagtat ggtagccagt
    14581 tgtttatgtc ttagcccaag tctgttaaag aaaaagttga aaagcgaaaa caccagttat
    14641 agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa atgaattaat gatggacggt
    14701 aaaaatatct ctcaggtatc acagtcctgc ggctacaaca gtacgtcgta ctttatttct
    14761 gtctttaaag acttctacgg tatgacgccg ctgcattatg ttagtcagca cagagaacgc
    14821 actgtcgcct gatttttaac cttaacgaag agctatatta ataacggcat cagcgataac
    14881 ccggtcgata ataattcaac tatcgaatgc aggcgtatga tatgacgtaa tttattgtca
    14941 cgaagctcgc cttcgcagga gtttaattat gtcttcggat gctgatgctc acaaagtggg
    15001 cttaatcccc gtcaccctga tggtgtcggg gaatattatg gggtcaggtg tttttctgtt
    15061 acctgcaaac ctggcctcca ctggcgggat tgccatttat ggatggttgg tgacgattat
    15121 cggtgcgctg gggctctcga tggtatacgc caaaatgtcg ttcctcgacc caagtcctgg
    15181 tggttcttac gcttacgccc gccgctgctt tggcccgttt ctcggttatc aaaccaacgt
    15241 cctctactgg ctggcctgct ggatcggcaa tatcgccatg gtggtcattg gcgtaggata
    15301 tttaagttac ttcttcccga ttctgaaaga tccattggta ttaaccatca cctgcgtcgt
    15361 ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg aaaatgatca cccgcgtgca
    15421 ggccgttgcc actgtgctgg cgctgatccc catcgtcggg attgccgtat ttggctggtt
    15481 ctggttccgt ggtgaaacct atatggcggc atggaacgtc agcggcctgg gcaccttcgg
    15541 tgcgattcaa agtaccctta acgttacgct gtggtcgttc atcggtgtgg aaagtgcctc
    15601 cgttgccgca ggtgtggtga aaaacccgaa acgcaatgtc cctatcgcca ccattggtgg
    15661 ggtattgatt gccgccgttt gctatgtgct ctccaccacc gcgattatgg ggatgatccc
    15721 taatgccgca ctgcgcgttt ctgcctcgcc attcggtgat gccgcacgga tggcgttggg
    15781 tgacaccgct ggggccattg tttccttctg cgcagctgcg ggttgcttag gttcactggg
    15841 cggctggacg ttgctggcgg gtcaaacggc gaaagccgct gccgatgacg gactgttccc
    15901 accgattttt gcccgtgtaa ataaagcggg tacgccagtg gcggggctga ttatcgtcgg
    15961 tattttgatg accatcttcc agctcagcag catttcacca aacgcgacca aagagttcgg
    16021 tctggtttct tccgtctcgg tcatctttac actggtgcca tatctttaca cctgtgcggc
    16081 gttactgctg ctcggccacg gtcactttgg taaagcacgc ccggcatatc tggctgttac
    16141 taccattgcc ttcctctact gcatttgggc cgtggtgggg tccggggcga aagaggttat
    16201 gtggtcattt gtcaccctga tggtcatcac cgccatgtat gcgctgaatt acaaccggct
    16261 acataaaaac ccgtatccct tagatgcacc aataagcaaa gattaattcc ccttaatcca
    16321 gcaaacataa aagccaacct taagaactta aggttggctt aattttgctt tgcgagcata
    16381 tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg cctactaaaa agaccctctt
    16441 tgaatttact cgcctggcta ttgttggccg ctttttatat ctctatctgc ctgaatattg
    16501 ccttttttaa acaggtgttg caggcgctgc cgctggattc gctgcataac gtactggttt
    16561 tcttgtcgat gccggtcgtc gctttcagcg tgattaatat tgtcctgaca ctaagctctt
    16621 tcttatggct taatcgacca ctggcctgcc tgtttattct gattggcgcg gctgcacaat
    16681 atttcataat gacttacggc atcgtcatcg accgctcgat gattgccaat attattgata
    16741 ccactccagc agaaagttat gcgctgatga caccgcaaat gttattaacg ctgggattca
    16801 gcggcgtgct tgctgcgctg attgcctgct ggctaaaaat caaacctgcc acctcgcgtc
    16861 tgcgcagtgt tcttttccgt ggagccaata ttctggtttc tgtactgctg attttgctgg
    16921 tcgccgcact gttttataaa gactacgcct cgttgttccg caataacaaa gagctggtga
    16981 aatccttaag cccctctaac agcattgttg ccagctggtc atggtactcc catcagcgac
    17041 tggcaaatct gccgctggtg cgaattggtg aagacgcgca ccgcaacccg ttaatgcaga
    17101 acgaaaaacg taaaaatttg accatcctga ttgtcggcga aacctcgcgg gcggagaact
    17161 tctccctcaa cggctacccg cgtgcaacta acccgcggct ggcgaaagat aacgtggtct
    17221 atttccctaa taccgcatct tgcggcacgg caacggcagt ttcagtaccg tgcatgttct
    17281 cggatatgcc gcgtgagcac tacaaagaag agctggcaca gcaccaggaa ggcgtgctgg
    17341 atatcattca gcgagcgggc atcaacgtgc tgtggaatga caacgatggc ggctgtaaag
    17401 gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct gaatctgcct gatcagtgca
    17461 tcaacggcga atgctatgac gaagtgctgt tccacgggct ggaagagtac atcaataacc
    17521 tgcagggtga tggcgtgatt gtcttacaca ccatcggcag ccacggtccg acctattaca
    17581 accgctatcc gccgcagttc aggaaattta ccccaacctg cgacaccaat gagatccaga
    17641 cctgtaccaa agagcaactg gtgaacactt acgacaacac gctggtttac gtcgactata
    17701 ttgttgataa agcgattaat ctgcttaaag aacatcagga taaatttacc accagcctgg
    17761 tttatctttc tgaccacggt gaatcgttag gtgaaaatgg catctatctg cacggtctgc
    17821 cttatgccat cgccccggat agccaaaaac aggtgccaat gctgctgtgg ctgtcggagg
    17881 attatcaaaa gcggtatcag gttgaccaga actgcctgca aaaacaggcg caaacgcaac
    17941 actattcaca agacaattta ttctccacgc tattgggatt aactggcgtt gagacgaagt
    18001 attaccaggc cgcggatgat attctgcaaa cttgcaggag agtgagtaaa tgaaaattct
    18061 gattgttgaa gacgatacgc tgttattgca gggactgatt ctggcggcgc aaaccgaagg
    18121 ctacgcgtgc gatggcgtga caaccgcgcg gatggcggaa caaagccttg aggacggtca
    18181 ttacagcctg gtggtactgg atttagggtt acccgacgaa gatggactgc attttctcgc
    18241 ccgtatccgg cagaaaaaat acaccctgcc ggtactgatc ctcaccgctc gcgatacgct
    18301 gaccgacaaa atcgccgggc tggatgtcgg tgccgatgac tatctggtga agccttttgc
    18361 gctggaagag ttacacgccc gtatccgcgc cctgctacga cgccataata atcagggcga
    18421 aagtgagctg attgttggca atctgacgct gaatatgggt cgccgtcagg tatggatggg
    18481 cggtgaagag ttgattctga cgcccaaaga atatgctctg ctgtcacggt taatgctcaa
    18541 agcaggcagt ccggtacatc gggaaattct ctacaacgac atctataact gggacaatga
    18601 accctcgacc aacaccctgg aagtgcatat ccacaatctg cgcgacaaag tgggcaaagc
    18661 ccgtatccgc accgtgcgcg gctttggcta tatgctggtc gcgaatgagg aaaactaatt
    18721 gaatctgatg cgttttctgc gccgaccaat atcgctgcgc caacggctga tattgaccat
    18781 cggggccatt ttgttggtgt ttgagctgat cagcgtcttc tggctatggc atgaaagtac
    18841 cgagcagatt cagctgtttg agcaggcgct acgcgacaat cgcaacaacg atcgccacat
    18901 catgcgtgag atccgcgagg cggtcgccag cctgattgtc cccggcgtct ttatggtcag
    18961 cctgacgcta tttatctgct atcaggcggt acgccgcatc acccgcccgc tggcggagct
    19021 gcaaaaagag ctggaagcgc gcaccgccga caacttaacg cccattgcca ttcacagcgc
    19081 caccctcgaa atcgaagcgg tggtttcggc gttgaacgat ctggtcagtc gcctgaccag
    19141 cacgctggat aacgaaaggt tgtttaccgc tgacgtcgcg cacgaactgc gaacgccact
    19201 ggcgggggtg cgtttgcatc tggaactgct ggcgaaaacg catcacattg atgtagcacc
    19261 gttagtggca cggcttgatc agatgatgga gagcgtctcc cagctgctgc aactggcgcg
    19321 tgccggacag tcattttctt ccggtaatta tcaacatgta aaactgctgg aagatgtcat
    19381 tctcccctcg tatgacgaac tcagtaccat gctcgaccag cgacagcaaa ccctgctatt
    19441 gccagagagc gccgccgaca tcaccgttca gggtgatgca accctgctgc ggatgttatt
    19501 gcgaaacctg gtagaaaacg cccatcgtta cagcccgcaa ggcagcaaca ttatgattaa
    19561 gctgcaagaa gatggcggag cggtcatggc agttgaagat gaaggaccag gtattgatga
    19621 gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg gacagccgtt atggcgggat
    19681 tggtctgggg ttaagtattg tcagccgcat tacacagttg catcacgggc agtttttcct
    19741 gcaaaaccgg caagagactt ccggcacgcg ggcctgggta cggctgaaga aagatcagaa
    19801 cgtggcaaac cagatataaa gaaagctgct gaccaccagc acgctgaaca cggtagttaa
    19861 actttcataa acacggtttt tcattgcact ctcctccgtt aacctggagg agagtatgcg
    19921 cgtcagagat taagtgaacc ttaagagttc aattggcctg ataagacagc gtcacatcag
    19981 gccatccgtt tcagcttatt catcaattcg cggatgttgc tgcaccaggc gggtacgttt
    20041 cgcctgcaaa tcggcaatct cgtggtcaat attatcgatt ttctgctcga tattatcgta
    20101 atgctcgacg agaatttcct tcgcttcctg tatatctgac gccgccggtg tcgcaccttt
    20161 caacggacga tttgccgtct ctttcatggt tacgccggtg attaaaccaa tcaccgccac
    20221 taccatcagg taataggcag gcatcatcag attctgcgag ctttcgacca gccaggccgc
    20281 cagtgttggc gtcagaccgg caaccagcac cgaaatatta aatgccgccg ccagcgcgct
    20341 gtaacggata tgcgtcggga acatcgctgg caaggtagaa gccataacgc ccataaagca
    20401 gttaaggatc accgccagca tcagtaaccc ggcaaaaatc aggccgatga cgttactgtt
    20461 aatcagaata aacgccggga tcgccaacac aaacagtgca acactaccaa gtagcacaaa
    20521 cggacgacgg ccaaaacggt cactcagcaa gcccatcacc ggctggacaa acagcatacc
    20581 gatcataatg gcgataataa tcagcacccc gtggtcttcg gagtaatgca ggttatgcga
    20641 caaataactc ggcatatagg tcagcaacat gtagtaagtc acgttggtgg caattaccag
    20701 accaatacat gtcaacaggc tgcgccagta tttagtggca atctctttaa acgagacttt
    20761 cgggccatcc tgcaaacctt cgcggtcgcc ctgttccagt ttatcaacat gctgctggaa
    20821 cgccggagtt tcttccagcg catggcgcag gtaaagcccg ataatcccta acggcagagc
    20881 aataaagaac ggaatacgcc agccccagtc gaggaagttc gcttcgccga caatggtcga
    20941 aattaacacc accacgcccg cacccagcac aaacccggca atagaaccga agtccagcca
    21001 gctgcccata aagccacgtt tacggtcagg ggagtattcc gcaacaaata tcgacgcccc
    21061 ggtatattca ccaccgaccg agaaaccttg tgccatctta cagatcaaca gcagaatcgg
    21121 tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca atacagaacg tactgatcga
    21181 cataatcaca atagtgatag cgaggatctt ctggcgacca tatttatcgc ccaacatacc
    21241 aaagaagagt ccgccaagcg gtcgaatcag aaagggaacg gagaaagtgg caagtgcagc
    21301 aaccatctgc acgctggggt cagcccccgg gaaaaaaact ttacctaatg cgtaagcaac
    21361 aaaaccataa acaccaaaat caaaccattc cattgcatta cccagtgatg ctgcggtaat
    21421 ggctttacgc agtttaccgt catcaataat ggtgacatca cgaagggtaa tcggttttac
    21481 ttttttcctt ttcagcatag ctttcctcgc agagttggcc catttccgca aacgccagaa
    21541 aaatcagcgc gacggccgca aaaggggagc gggaattgca ggatgcaatc tctgtaacaa
    21601 gcgtagcggg tttgcttaca ccctccggtt aaggaatgta caatcaaacc agttgacgcc
    21661 tgaattgggc agttaatgac ctttttaccc taccagtgtt taaatttgtg atcaacttca
    21721 cacatggatt tcgtttagga ctcattgatg taactgatta tttacgccaa atttacagag
    21781 aagatttcct cgaccattgc ccccaccaaa cgctgcgctg aacagagtcg tggcatcccc
    21841 agcgcgacag tttgccagcg ttgggtcact gaccagcaaa cgggttgttt atctgccgtg
    21901 gtccactttc ccagccaact taaaaaatca gcatgatccc cgcccgtcgg aacggtcgaa
    21961 gaggcaaacc actgatggta atagtgacgc gtagtcggtg ccgcattgat actctctgcc
    22021 agatgttgtg tcgctttctc gcgcaaatca cccagcaaca acaccaccgc ttgcgggctt
    22081 cccagacggc aggcatcgcc aaatgcaccc cagcgcaact cctccagaac gataaaacag
    22141 cgcccaggca acgaccaggc gttatacgcc ccagcacgcc agcgggtaaa aatttgctcg
    22201 ctctgatcac ctgcctgcac cagtaggcct cgttgcgtta acgccttttc cttccagcga
    22261 caacgggctg aaaaccactc atacaaggcg gcaatttgct gggggagctc cgggaaagca
    22321 gagtgctggc ggtgatccat tttttgcagc agcgccagat gctgcgcgat ctgcgtcagc
    22381 gccagatgac cgggatccag catcgacgcc agtttttcca ccattgtcag tcgcaggcta
    22441 agcggcgcat ttaacatgcc gccgttcgtc catgtctgaa gctgcacacg cgaaagaagt
    22501 tcctgcatta gtcgttcacg aaactgctgc tgatgggctt gaggatggcg ggcatcatcg
    22561 ccctgcgcca gatccactaa aaagcgcgga tgaaccgact ccagcacgcg accggggccg
    22621 tccagtaacg tcttggtcaa tatcgttctg ccgtgaaaag tgtttgaata tcatcgcgta
    22681 acagctgagc gtcggtgtaa atccagctgt gagtcatcac agtctgctgc aattgctgct
    22741 gcatcagcct gaccaccgat tcattttgtt gacgcagagc cagactttcg cgtaaacgcg
    22801 tctgtaattc tgtcaaacat gaagcgaact cagcgaaaaa agtattcatg cctgccgtaa
    22861 cagattcatc gacctgctct gccagaactt tagccatttg ttggcaatag agatcgactt
    22921 ctgcgcttaa tgctcgttgc aacacactgt aatcaaccgt ttctgtcggg gatttctcat
    22981 ttccccgtcc ccagtcgggc tgattcaacc agcgcgaaaa agtctcacgc acaacgccta
    23041 aacgcgtgct ctgctcgtcc gttgcttcct ggcgcgaaat gacttcactg aacagctggc
    23101 gagtgttgaa gtggggaact acgccgtgaa aaacaggaaa atgaaaccca ggacgaaacc
    23161 ctgactcgct caattccatt ttgacttgtt gctcaatggg gcgaataaca tcggttaaca
    23221 cccggcaaag ggtggattcc atctcggcaa aacgcagcgt aaagtcgcga ctgatggtgt
    23281 tctgcgccgt ctgtaacagt gtctcacagc gggtacgaat ctcgcttaac ggctccgaat
    23341 catcctgaaa caaggcggct aactgcgcat tcaccgcatc ttgctgttga cgcagaaagt
    23401 ggttggcgga ggtcagggcc agctcgattt catgtttaat ctcgccgctc acctgcgcct
    23461 gattgagttg caatagctgc aaactttctt cgacctgatg gatattttgc cgcaattgtt
    23521 cacaagcgac gtttaacccg tgcgcacgaa aatccaggta ttcccgcgcc tgctgcgcgt
    23581 aattcaacag tttatgcgca gcagatcgca gagcatacaa tgaggcgtta gcgtaagcgg
    23641 catgaagcaa cgcctgaatt ggctgggcga acagcgaatc ttcccacaac tgatcggcag
    23701 catgacgaat atgttcgagg tccgccagat cggcatgacg ccagcgcctg ccgagcgcgg
    23761 catgggcaaa atcttccacc cagcgttgtt gctctggcgc tggtaactta ccgttgttgg
    23821 ctaactcata gcgcgcccga ttcgccaggt agccccacat cgacgacacc gggaatatct
    23881 gctgtggcgt aatacagcct ttcatcagcg tcccggaaat cagtgcccgc acctggtcgg
    23941 cgtcgtcact gttacgatcc tgttgatcga acttattgac cagcacatac agcggcaccg
    24001 attgccccac cgccaaaatc gcctcacgga cctcttcatc ggagatcgat ttcagttgcg
    24061 tataatccag caccgccagt accgccgagg cgcgtgccag ctgctggtta agcatttttt
    24121 gcagatgcgg ttgcccggct tcatttggcc cgggggtatc cagtaacgtc aactgaccgg
    24181 gataactttc cagccccgcc agatggacaa actccacttc aatcacggga atatgctcaa
    24241 tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc cttcgccagt cgcactaaat
    24301 cattcaaact tttcagacaa tgaaaaatag gctgggcacc cagataatat ttttcgaaag
    24361 cgacgccatt ttcgatccgc tgcataagcg cacgcatatc tttatctatt tccagcacat
    24421 cggtcagatg cttaatatcg caatcacgca ggcgctgttg taattgttga attaaacaat
    24481 cgattggcgc gacatgtgaa aaatgcagta ccggttcctt ttgcccgggc gtatggcgaa
    24541 taagcgtcgg cagcgcagtc attgggcgat tacgattagg cagaacctcc gtaccaacaa
    24601 tggcattaat ggtggttgat ttccctgctt tcatggtacc gacaattgcc agcaccattt
    24661 ccagtcggga aattttacac aactcattat tcagcatcgc gtgacgttcg gcgatattag
    24721 gctgactcca gggtaaagcc agttgtggcg cgtcgtctcc gggtacagag agaggcattt
    24781 tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag gcgttcagcc tcctgactta
    24841 actcatacag ggtctgtgtg tacatagaaa attcttcctt aaagcaaatt ttgttacttt
    24901 tatttagcca gattgttttt gagttctgtt ttcggctttt ataattactg caagaaataa
    24961 ttttatattt agtgtgttgt tttttatcag aataaataac gtcttctgat gcgtttaaaa
    25021 cgtcagaaag ataaaaatat catgtgaata aaaaaaagaa caagtagagc attaacatta
    25081 tcttaaataa taaatagagg caaaaagatt attttctttt tacgtttcct ttcaaatgaa
    25141 aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact cagggatttt gtgtataatt
    25201 gcggcctttt tcggcaatct gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa
    25261 atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacactta cgaagataac
    25321 ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acgcagaacc tgcacaggaa
    25381 agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg cgacagcgtt
    25441 accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa
    25501 gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat cgacggtttt
    25561 ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg tgatcggtaa
    25621 gccggataag gcgctcgcgc cgcatccggc aacgatgccg actgcctgat gcgacgcttg
    25681 cgcgtcttat caggcagcct ctcatctgta taaatttcga actacactta actggcttct
    25741 cttaactgag gtcaccatca tgccgttaag tccctacctc tcttttgccg gtaactgttc
    25801 cgacgcgatt gcctattatc aacgtacgtt gggcgcggaa ctgctctata aaatcagctt
    25861 cggcgaaatg ccgaaatcag cgcaggacag cgccgagaac tgcccttccg gaatgcaatt
    25921 tcccgatacc gccatcgctc atgccaacgt gcgcattgcc ggaagcgaca tcatgatgag
    25981 cgatgctata ccgtcaggaa aagccagcta ctccggcttt acgctggtgc tcgattcgca
    26041 acaggtcgaa gaaggaaaac gctggtttga taaccttgcc gctaacggaa aaatcgaaat
    26101 ggcctggcag gaaactttct gggcgcatgg ctttggcaaa gtcaccgata aatttggcgt
    26161 accgtggatg attaatgtcg tcaaacaaca accaacgcaa taacccgccg ggaggcccgc
    26221 cctcccgcac tgtcatcgaa ttcccgttaa ctcttcatct gttagtcact tttaattaac
    26281 caaatcgtca caataatccg ccacgatgga gccacttttt tagggaggct gcatcatgca
    26341 aacgattatc cgtgtcgaga agctcgccaa aaccttcaat cagcatcaag cgctgcatgc
    26401 ggttgatctg aacattcatc acagtgaaat ggtggctctg cttgggccgt cgggttccgg
    26461 caaatccacc cttttacgtc atttaagcgg tttgattacc ggcgataaat ccgccggtag
    26521 ccatatcgag ctactgggcc gcactgtcca gcgcgaaggc cgcctggccc gcgatatccg
    26581 caaaagccgc gccaacaccg gctacatctt tcaacaattc aacctggtga accgcctgag
    26641 cgtactggag aacgtgctga ttggcgcgct cggcagcacg ccgttctggc gcacctgttt
    26701 tagctggttc accggcgagc agaaacagcg cgcgttacag gcgctgaccc gcgttggcat
    26761 ggtgcatttt gcccatcagc gcgtttccac cctctccggc ggccagcagc aacgtgtggc
    26821 gattgcccgt gcgctgatgc agcaggcgaa agtgattctg gccgatgaac ccatcgcctc
    26881 gctggacccg gaatcagcgc gcatcgtgat ggacaccctg cgcgacatca accagaacga
    26941 cggcatcacc gtggtcgtca cgctgcatca ggtggattac gccctgcgct actgcgaacg
    27001 catcgtcgcc ctgcgtcagg ggcacgtctt ctacgacggc agcagccaac tgtttgataa
    27061 cgaacgtttt gaccatctct accgcagcat taaccgcatc gaagagaacg cgaaagctgc
    27121 ctgatatccc catcattgag gaaaacgaat gaacgctaag ataattgcct cgctggcctt
    27181 caccagcatg ttcagcctca gcaccctgtt aaccccggca cacgccgaag agcaggaaaa
    27241 ggcgctgaat ttcggcatta tttcaacgga atcacagcaa aacctgaaac cgcaatggac
    27301 gccgttttta caggatatgg agaagaaact gggcgtgaag gtcaacgcct tctttgcccc
    27361 ggactacgcg ggcattatcc aggggatgcg cttcaataaa gtggatattg cctggtacgg
    27421 caacctgtcg gcgatggaag cggtggatcg cgccaatggc caggtcttcg cccagacggt
    27481 cgcggcggat ggatcgccgg gttactggag cgtgttgatc gtcaacaaag acagcccgat
    27541 caacaacctg aacgatctgc tggcgaagcg gaaagatctc accttcggca atggcgatcc
    27601 taactccacc tctggcttcc tcgtccccgg ttactacgtc ttcgctaaaa acaatatctc
    27661 cgccagcgac ttcaagcgca ccgtcaacgc cgggcatgaa accaacgcgc tggccgtcgc
    27721 caacaagcaa gtggatgttg ccaccaacaa caccgaaaac ctcgacaagc tgaaaacctc
    27781 cgcgccggag aagcttaaag agctgaaggt gatctggaaa tcgccgctga tcctaggcga
    27841 tccgatcgtc tggcgcaaga atctgtccga aactaccaaa gacaagatct acgacttctt
    27901 tatgaactac ggaaaaacgc cggaagagaa agcggtgctg gaacgcctgg gctgggcgcc
    27961 gttccgcgct tccagcgacc tgcaactggt gccgattcgc cagctcgcac tgtttaaaga
    28021 gatacaaagc gtgaaaggca ataaagggct gaatgagcag gacaagctgg cgaaaaccac
    28081 cgagattcag gcgcagctgg atgacctgga ccgcctgaac aacgcgttaa gcgcgatgag
    28141 ttcggtgagt aaagcggtgc agtaaatcgt aggtcggata agacgccccg gcgtcgcatc
    28201 cgacaatgtg taggcgttga tgccggatgc ggtgcaagca ccttatccgg cctacagacc
    28261 ggagccaaac atgcaaacca tcaccatcgc cccacccaag cgtagctggt tctcgcttct
    28321 gagctgggcc gttgttctcg acgtgctggt cgtctcgtgg cagggcgcgg aaatggcccc
    28381 gcttacgctg attaaagacg gcggcaacat ggcaacctt
//
LOCUS       DBKELA010000052        28368 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.052, whole genome shotgun sequence.
ACCESSION   DBKELA010000052 DBKELA010000000
VERSION     DBKELA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.052"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            233..340
                     /locus_tag="ACUQR0_002170"
     CDS             233..340
                     /locus_tag="ACUQR0_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HHV9109197.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            716..823
                     /locus_tag="ACUQR0_002171"
     CDS             716..823
                     /locus_tag="ACUQR0_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HHV9109198.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(910..2589)
                     /gene="bcsG"
                     /locus_tag="ACUQR0_002172"
     CDS             complement(910..2589)
                     /gene="bcsG"
                     /locus_tag="ACUQR0_002172"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HHV9109199.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2586..2777)
                     /gene="bcsF"
                     /locus_tag="ACUQR0_002173"
     CDS             complement(2586..2777)
                     /gene="bcsF"
                     /locus_tag="ACUQR0_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HHV9109200.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2774..4345)
                     /gene="bcsE"
                     /locus_tag="ACUQR0_002174"
     CDS             complement(2774..4345)
                     /gene="bcsE"
                     /locus_tag="ACUQR0_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HHV9109201.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4618..4806
                     /gene="bcsR"
                     /locus_tag="ACUQR0_002175"
     CDS             4618..4806
                     /gene="bcsR"
                     /locus_tag="ACUQR0_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HHV9109202.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4818..5570
                     /gene="bcsQ"
                     /locus_tag="ACUQR0_002176"
     CDS             4818..5570
                     /gene="bcsQ"
                     /locus_tag="ACUQR0_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HHV9109203.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWWQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5567..8184
                     /gene="bcsA"
                     /locus_tag="ACUQR0_002177"
                     /pseudo
     CDS             5567..8184
                     /gene="bcsA"
                     /locus_tag="ACUQR0_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8195..10534
                     /gene="bcsB"
                     /locus_tag="ACUQR0_002178"
     CDS             8195..10534
                     /gene="bcsB"
                     /locus_tag="ACUQR0_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HHV9109204.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            10541..11647
                     /gene="bcsZ"
                     /locus_tag="ACUQR0_002179"
     CDS             10541..11647
                     /gene="bcsZ"
                     /locus_tag="ACUQR0_002179"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HHV9109205.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            12267..13958
                     /gene="bcsC"
                     /locus_tag="ACUQR0_002180"
                     /pseudo
     CDS             12267..13958
                     /gene="bcsC"
                     /locus_tag="ACUQR0_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            14040..16028
                     /gene="hmsP"
                     /locus_tag="ACUQR0_002181"
     CDS             14040..16028
                     /gene="hmsP"
                     /locus_tag="ACUQR0_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HHV9109206.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16214..17500
                     /gene="dctA"
                     /locus_tag="ACUQR0_002182"
     CDS             16214..17500
                     /gene="dctA"
                     /locus_tag="ACUQR0_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HHV9109207.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17721..19217
                     /locus_tag="ACUQR0_002183"
     CDS             17721..19217
                     /locus_tag="ACUQR0_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HHV9109208.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(19313..20242)
                     /gene="kdgK"
                     /locus_tag="ACUQR0_002184"
     CDS             complement(19313..20242)
                     /gene="kdgK"
                     /locus_tag="ACUQR0_002184"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HHV9109209.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20474..21241
                     /gene="pdeH"
                     /locus_tag="ACUQR0_002185"
     CDS             20474..21241
                     /gene="pdeH"
                     /locus_tag="ACUQR0_002185"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HHV9109210.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            21311..23371
                     /locus_tag="ACUQR0_002186"
     CDS             21311..23371
                     /locus_tag="ACUQR0_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HHV9109211.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23553..24875)
                     /locus_tag="ACUQR0_002187"
     CDS             complement(23553..24875)
                     /locus_tag="ACUQR0_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109212.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25286..26299)
                     /gene="yhjD"
                     /locus_tag="ACUQR0_002188"
     CDS             complement(25286..26299)
                     /gene="yhjD"
                     /locus_tag="ACUQR0_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HHV9109213.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGATITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26348..27247)
                     /locus_tag="ACUQR0_002189"
     CDS             complement(26348..27247)
                     /locus_tag="ACUQR0_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHV9109214.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            27767..>28368
                     /locus_tag="ACUQR0_002190"
     CDS             27767..>28368
                     /locus_tag="ACUQR0_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHV9109215.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6772 a         7145 c         7794 g         6657 t
ORIGIN      
        1 agacgtgacg gattccggaa cagaaaaaca gtcgtagaat cagcgtcaag ccggaagggt
       61 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggagagag
      121 aaaacccccg cacgttgcag gtatgcacct gacaacatca cgggggccat tcttgaacct
      181 gaacaactca agaatagccg cgaaacgttg tcattacaac acaggcggct atatgacgct
      241 cgcagaactg ggcatggcct tctggcatga tttagcggct ccggtcattg ctggcattct
      301 tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa cgtgtcatgc gggcgtcagg
      361 ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg gtgaaactct gcggcctttt
      421 tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct gcggaatgcg cagcacagag
      481 tgaagacgtg acggattccg gaacagaaaa acagtcgtag aatcagcggc aagccggaaa
      541 ggttccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg ttgctggaga
      601 gagaaaaccc ccgcacgttg caggtatgca cctgacaaca tcacgggggc taatcttgaa
      661 cccaaacaac tcaagaatag ccgcgaaacg ttggcattac aacacaggcg gctatatgac
      721 gctcgcagaa ctgggcatgg ccttctggca tgatttagcg gctccggtca ttgctggcat
      781 tcttgccagt atgatcgtga actggctgaa taagcggaag taacgtgtca tgcgggcgtc
      841 aggctgccgt aatggcaatt tgcgcccgga tcaggccgca gaggttaaac tctgcggcct
      901 ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg ttcaggcgaa cgtacggttt
      961 atcttggtat tgaataacta ctgcatttga gttctcggag accggtgctg tttgtggcaa
     1021 cccactggtg agttttttcc agtcaacatt gtcttcggtg aaaatcttgc catcaagaac
     1081 gcgaaccacc agatcggaga tagccaggaa gctgctcggt tggtcaatga caatcggtgc
     1141 cccctgatgt ggtgccttca tgccgaagaa tttcacccca acggggacgt ctgtgataga
     1201 cgggctaggg atatcacgta ggccagatac ctgcattctg tcgcccttca gcgcgccgcc
     1261 gtgttccggc accacgacca ccatcacttt acgacccgat ttctccagtt cagtaaagaa
     1321 ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg taatccgctg ttttgctgac
     1381 ccccggataa tggttgccgt catgcagtgg aagcgtgttg tagaacgtgg cactacggct
     1441 gtttttatct ttttcggtaa cgtccagcca gcggttaagc acggcggtat cgtcataaac
     1501 cggcgaacca tcaaagccca gcaaaataac cggcagattt gtttgatcca tcaattcagt
     1561 ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg ccgaactggc cgttatgtcc
     1621 catcatcagg tgctgggtaa agcccagttt cgaaaggtta tcaaacagat agcagtcgtt
     1681 atttgccggt tgatacagat tagtgtgcga agtctgcccg cagctggcgc gcagtaaacg
     1741 gatcgccgcc gggccactgt aggaggtggc ggagttaaag ttcttgaaat caatatcgaa
     1801 atgcgaccac agtggatgcg acatcaaccc ggcggcttct atatccgacc aggaaagcga
     1861 acagatgtta atcaccagta gttcaaatgg ctgagcatca gcgggcagcg aagacgggaa
     1921 ggtcgattta cgtttcgcct ccgcgttata gaaattattc agccaggcgt taaggttcgc
     1981 cgttgttggc ggtgcagttt gtgcgggcat atcacccact accggtgcgc cacccgtcgc
     2041 cgcaacggtt gccgctgcgt taccacccgt cgttgttaca gtggtcgtcg gttgtccggc
     2101 tggccacaag gagaaacttg gtcccgccaa ggtaagtacg ttcagccata gcagtatggc
     2161 aaccacaaaa acggtaatgc gaatccattg tgacaggaat aaccaggcca ctaataaaac
     2221 aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg acaaggtcga ttaaataatc
     2281 ggtactgaac cccgccacct gcgaaccctg gctcattatg ctttccgggc caggcaacca
     2341 gatgtcatgc cagaacaaag caaagccgat cggcagggca atccagtggc gcaagcgatg
     2401 caggctgtaa cgcggaatgg gcatcagcag aaacgcggca aacaccaaat tgaggagcgg
     2461 gtggaagtta agatatcccg cccacaacag gccaaactta accagaaaat agaagttcca
     2521 gccggaaagg ccgcgccagt attgccagag ggaagaaggc atggcggtat tttgcgtaaa
     2581 ttgagtcatt ttttggttgc cctggctttt tccgtgcggc gtaacgtccc ggccggttta
     2641 acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc gaatgcgtcg caaagagtgc
     2701 cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa cgataataat ttcaatgata
     2761 tcgctgatgg tcatcatgat gagcgctcca cagcatcatc taacagtcgc atgggttctg
     2821 gtattcgtcg ccagtggcga ccatcgtgct cggcattgat gaccggttta gaactttgcg
     2881 ttaaaggcag cggcattccc cattgttctg gggcaagcag gcgcatctgc accagctcgg
     2941 cactgatttg atcatcttca aaccagacca tacggtttga gaaaatgtcg ccagtaggca
     3001 atgggaaaat atgattcaac gcggtatcca gatcgttaat ccgacagaat gagagaaaca
     3061 gcaccagccg attaccgcca atggtcatga tatcgccggt acggttaggg cgacatagcg
     3121 tcagggcttg ctcaacgcgg atacccggta ccggacgtag ggcaaccaga acgcctttac
     3181 cgtgggcagg caagagaggg ttattcatca tgttgttgac ggcattacag aacacatccc
     3241 acttctggaa accacgcagt ttgagcggct gggtcattga cagcaaggta gtgatatctt
     3301 ccggtacata gcgactaaac ttctgccctt gcacgctttc gatcatcgtc agacaacggg
     3361 agagtggcgc attccacgga ataaccatat ttgcaccgca ggccaataac aaacgttcat
     3421 cggtggcgcg caggctagcg gtattttccc gcacgaggat tttcatcgca ctaccgcgct
     3481 ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat ttgcgcattt tgctgtaaag
     3541 aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt gaacaggact tcgttattgt
     3601 tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc cagtacagca acattactca
     3661 gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc ttcgctttga actaatgtcc
     3721 agataccatt ttgttgctga acgctaagct gctgacgggc gctgacccct ttttcgttgc
     3781 accagaaggc aatatccagc aaatgttggt caccctgaaa acgcaaactg gcaagaccaa
     3841 aaagtgaacg gtattcctca agcaataatg aaaattgttt atcgttatta tttccgggat
     3901 taattaccaa aagcgaacaa tggtttaacc tgctccattt attcatttta tccaaccatg
     3961 agcgaagccg ctcggcagga atattttgcc atgcgttatt tgcgcaaaca agaataaaaa
     4021 ggtaattatg gggatcaata gaacactgca aatcacgggc caaatagtat agaccttttt
     4081 catgatttag cattgaaaat aattttattt tttccggccc ttgagaatca tctaattgaa
     4141 agattttcgc cggatcgctg tccatgctaa tgaccgcgac gtgtgcggtt tcagcctggg
     4201 atgcaattgt ttgattcgcc agactgatag catcttcatg gcgatcgacg ttaaaccacc
     4261 agacgccgcc tgctggcata tgtcgcagct catcccataa tgatgagata ccgatagaga
     4321 atacagggtc cacaatgtcc ctcatgtcac cattttgtct gattatcagt ttactagcga
     4381 aagctaagaa ataaacctac cattgaaatt aaaacttatc agatttagca tgtgaatcaa
     4441 tttagaaggt cacaagacgt gtgattttag tcttttcgtt tttaactttt tctgttgcgt
     4501 aacggaaagt caaaaagtga gcaaattccc gtctcgccgt aaacaataaa ccggcgaaat
     4561 gctcacggtt agaattgtct catgaatggt acggttattt catagggatc aagcaaaatg
     4621 aataacaatg aaccagatac tctgcctgat cccgcgatag gctatatctt ccagaatgat
     4681 attgtggcgt taaagcaggc attttcactg cctgatattg attatgccga tatttcccaa
     4741 cgcgaacagt tggccgcggc attaaaacgc tggccgttgc tggcagagtt tgcgcaacaa
     4801 aagtagggga ttggtgaatg gccgtactgg gattgcaggg ggtgcgggga ggcgtgggga
     4861 caacaaccat caccgccgca ttagcctggt cattacaaat gttgggagaa aatgtcctgg
     4921 tggtcgatgc ctgcccggac aacttgttgc gcctgtcatt taatgttgat tttacccacc
     4981 gtcagggctg ggccagagcg atgctggatg gccaggactg gcgtgacgct gggttgtgct
     5041 acacctcgca gctcgatttg ctgccttttg gtcagttatc cattgaagaa caagaaaatc
     5101 cacagcactg gcaaacccgg ctgagcgata tttgctccgg cttacagcaa ctaaaagcca
     5161 gcgggcgtta ccagtggatt ttaatcgact taccgcgtga tgcctcgcag ataacccacc
     5221 agctgctgag tttgtgcgag cactcgctgg caatcgtcaa tgtggatgcc aactgccata
     5281 tccgactgca tcagcaagcg ctgccggatg gcgcacatat tttgattaat gacttccgta
     5341 ttggcagtca ggttcaggac gatatttacc agctttggtg gcaaagccag cgccgattac
     5401 tgccgatgct cattcatcgt gatgaagcga tggctgaatg cctggcggct aagcaaccag
     5461 taggtgaata tcgcagtgat gcgctggcgg ctgaagagat actgacgctg gcgaactggt
     5521 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     5581 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     5641 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     5701 gatttttatt ccgctggagc acccgcgctg gcagcgtatt cgcgcagaac ataaaaacct
     5761 gtatacgcat atcaacgcct cgcgtccgcg tccgctggac ccggtccgtt atctcattca
     5821 aacatgctgg ttattgatcg gtgcatcgcg caaagaaacg ccgaaaccgc gcagcgggca
     5881 ttttcaggtc tgcaaaatat tcgtggacgt taccatcaat ggatgaacga gctgcctgag
     5941 cgcgttagcc ataaaacaca gcatctggat gaaaaaaaag agctcggtca tttgagtgcc
     6001 ggggcgcggc ggttgatcct cggtatcatc gtcaccttct cgctgattct ggcgttaatc
     6061 tgcgttactc agccgtttaa cccgctggcg cagtttatct tcctgatgct gctgtggggg
     6121 gtagcgctga tcgtacggcg gatgccgggg cgcttctcgg cgctaatgtt gattgtgctg
     6181 tcgctgaccg tttcttgccg ttatatctgg tggcgttaca cctctacgct gaactgggac
     6241 gatccggtca gcctggtgtg cgggcttatt ctgctcttcg ctgaaacgta cgcgtggatt
     6301 gtgctggtgc tcggctactt ccaggtagta tggccgctga atcgtcagcc ggtgccattg
     6361 ccgaaggata tgtcgctgtg gccgtcagtg gatatctttg tcccgactta caacgaagat
     6421 ctcaatgtgg tgaaaaatac catttacgcc tcgctgggta tcgactggcc gaaagacaaa
     6481 ctgaacatct ggatcctcga tgacggcggc agggaagagt ttcgccagtt tgcgcaaaac
     6541 gtgggggtga aatatatcgc ccgcaccact catgaacatg cgaaagcggg caacatcaac
     6601 aatgcgctga aatatgccaa aggcgagttc gtgtcgattt tcgactgcga ccacgtacca
     6661 acgcgatcgt tcctgcaaat gaccatgggc tggttcctga aagagaaaca gctggcgatg
     6721 atgcagactc cacaccactt cttctcgccg gacccgtttg aacgcaacct gggacgtttt
     6781 cgtaaaacgc cgaacgaagg cacgctgttc tatggtctgg tgcaggatgg caacgatatg
     6841 tgggacgcca ctttcttctg cggttcctgt gcggtgattc gtcgtaagcc gctggatgaa
     6901 attggcggca ttgctgtcga aaccgtgact gaagatgcgc atacttctct gcgtttgcac
     6961 cgtcgtggct atacctccgc gtatatgcgt attccgcagg cggcggggct ggcgaccgaa
     7021 agtctgtcgg cgcatatcgg tcagcgtatt cgctgggcgc gcgggatggt ataaatcttc
     7081 cgtctcgata acccgctcac cggtaaagga ctgaagtttg ctcagcggct gtgctacgtc
     7141 aacgccatgt tccacttctt gtcgggcatt ccacggctga tcttcctgac tgcgccgctg
     7201 gcgttcctgc tgcttcatgc ctacatcatc tatgcgccag cgttgatgat cgccctattc
     7261 gtgctgccgc atatgatcca tgccagcctg accaactcga agatccaggg caaatatcgc
     7321 cactctttct ggagtgaaat ctacgaaacg gtgctggcgt ggtatatcgc accaccgacg
     7381 ctggtggcgc tgattaaccc gcacaaaggc aaatttaacg tcaccgccaa aggtggactg
     7441 gtggaagaag agtacgtcga ctgggtgatc tcgcggccct acatcttcct tgttctactc
     7501 aacctggtgg gcgttgcggt aggcatctgg cgctacttct atggcccgcc aaccgagatg
     7561 ctcaccgtgg tcgtcagtat ggtgtgggtg ttctacaacc tgattgttct tggcggcgca
     7621 gttgcggtat cggtagaaag caaacaggta cgccgatcga accgcgtgga gatgacgatg
     7681 cccgcggcaa ttgcccgcga agatggtcac ctcttctcgt gtaccgttca ggatttctcc
     7741 gacggtggtt tggggatcaa gatcaacggt caggcgcaga ttctggaagg gcagaaagtg
     7801 aatctgttgc ttaaacgcgg tcagcaggaa tacgtcttcc cgacccaggt ggcgcgcgtg
     7861 atgggtaatg aagttgggct gaaattaatg ccgctcacca cccagcaaca tatcgatttt
     7921 gtgcagtgta cgtttgcccg tgcggataca tgggcgctct ggcaggacag ctacccggaa
     7981 gataagccgc tggaaagtct gctggatatt ctgaagctcg gcttccgtgg ctaccgccat
     8041 ctggcggagt ttgcgccttc ttcggtgaag ggcatattcc gtgtgctgac ttctctggtt
     8101 tcctgggttg tatcgtttat tccgcgccgc ccggagcgga gcgaaacggc acaaccatcg
     8161 gatcaggctt tggctcaaca atgatgataa cgcgatgaaa agaaaactat tctggatttg
     8221 tgcagtggct atggggatga gtgcgttccc ctctttcatg acgcaggcga cgccagcaac
     8281 gcaaccactg atcaatgctg agccagctgt agccgcccag acggaacaaa atccgcaggt
     8341 ggggcaagtg atgtcaggcg agcagggagc tgatgccccc atcgtggcgc agaacggtcc
     8401 ttcgcgtgat gtgaagctga cctttgcgca aatcgcgccg ccgccgggca gcatggtgct
     8461 acgtggtatt aacccgaacg gcagcattga gtttggtatg cgcagcgatg aagtggtgac
     8521 gaaggcgatg ctcaacttcg aatacacgcc atcgccatcg ttactgcctg tccagtcgca
     8581 gttaaaggtt tatctcaatg atgagctgat gggcgtgctg ccagtgacca aagaacagtt
     8641 gggtaaaaaa acgctggcgc aaatgcccat taatccactg tttattaccg acttcaaccg
     8701 tgtgcggctg gagtttgtcg gccattatca ggacgtgtgc gaaaacccgg ccagcaccac
     8761 gctttggctg gatgttgggc ggagcagtgg actggatctg acctatcaga ccctgaatgt
     8821 gaagaatgac ctgtcacact tcccggtgcc attctttgac ccgcgcgata accgtactaa
     8881 caccttgccg atggtctttg cgggtgcgcc ggatgttggg ctgcaacaag catctgccat
     8941 tgtcgcctcg tggtttggtt cgcgttctgg ctggcgtggg cagaacttcc cggtgctcta
     9001 taaccaattg ccggatcgca atgccattgt ctttgcaacc aacgacaaac ggccggactt
     9061 cctgcgcgat catccggcgg taaaagcccc ggtgattgag atgattaacc atccgcagaa
     9121 tccttacgtc aaactgctgg tggtgtttgg tcgtgacgac aaagacctgt tgcaggcagc
     9181 gaaaggtatc gctcagggta acattctgtt ccgtggtgaa agcgtggtag tgaatgaagt
     9241 gaaaccgctg ctaccgcgta agccgtacga tgcgccgaac tgggtacgta ccgatcgtcc
     9301 ggtcacattt ggcgaactga aaacctatga agaacagtta caatccagcg gccttgagcc
     9361 agcagcgatt aacgtttcgc taaacctgcc gccggatctc tacctgatgc gcagtaccgg
     9421 cattgatatg gatattaatt accgctacac catgccgccg gtgaaagaca gttcgcggat
     9481 ggatatcagc ctgaataacc agttcctgca atccttcaac ctgagcagca aacaggaggc
     9541 gaaccgtctg ctgctgcgta ttccggtatt acaaggtttg ctggatggca aaacagatgt
     9601 ctctattccg gcgctgaaac tgggcgcgac caaccagctg cgcttcgact ttgagtatat
     9661 gaacccgatg ccaggcggtt cggtagataa ctgtattacc ttccagccgg tgcagaatca
     9721 tgtggtgatt ggtgacgatt ccaccatcga cttctcgaag tattaccact tcatcccgat
     9781 gccggatcta cgcgcctttg ctaacgcggg cttcccattc agccggatgg cggatctgtc
     9841 gcaaaccatc accgtgatgc cgaaagcgcc taacgaagca cagatggaaa cgttgctgaa
     9901 tactgttggt tttattggcg cacagacggg cttcccggcg attaatctga cggtgaccga
     9961 tgatggcagc accattcagg gcaaagatgc cgacatcatg atcatcggtg gtatcccgga
    10021 caaactgaaa gacgataagc agatcgacct attggtgcag gcgaccgaaa gctgggtgaa
    10081 aacaccgatg cgccagaccc cgttccccgg cattgtgccg gacgagagcg atcgcgcggc
    10141 agaaacccag tcaactctga cctcttccgg tgcgatggcg gcggtgattg gcttccagtc
    10201 gccgtataac gaccagcgca gcgtgattgc gctgttggca gatagcccac gcggttatga
    10261 aatgcttaac gatgcggtga acgatagcgg caaacgcgcc accatgttcg gttcggtcgc
    10321 ggtgatccgc gagtccggta tcaacagcct acgtgttggc gacgtttatt acgtaggtca
    10381 tctgccgtgg ttcgagcgct tgtggtatgc gctggcaaac catccgattc tgctggcggt
    10441 gctggcgact atcagtgtga tattgctggc atgggtactg tggcgtctgc tgcgaattat
    10501 tagtagtcgt cgtcttaacc cggataacga gtaattgaag atgaatgtgt tgcgtagtgg
    10561 aatcgtgacg atgctgttgt tggctgcctt tagtgttcag gcagcctgta cctggcctgc
    10621 ctgggagcag tttaaaaagg attacatcag tcaggaaggg cgcgtcatcg accccagcga
    10681 cgcgcgcaaa atcaccacct ccgaagggca aagttacggt atgttctttg ccctggcggc
    10741 taacgaccgt gcagctttcg ataatattct cgactggacg cagaacaatc tcgctcaggg
    10801 ttctttaaaa gaacgtttgc ccgcctggct gtggggcaag aaagagaaca gtaagtggga
    10861 agtgctggac agcaattcgg cctccgatgg tgatgtctgg atggcctggt cgttgctgga
    10921 ggcggggcgt ttgtggaaag agcagcgtta taccgacatc ggcagcgcgt tgctaaaacg
    10981 tatcgcgcgg gaggaagtgg tgacggtgcc tgggctgggt tccatgttgt taccgggcaa
    11041 agtgggtttt gctgaggata acagctggcg ttttaacccc agctacatgc cgccgacgct
    11101 ggcgcagtat ttcacccgct ttggcgcgcc gtggaccacg ctgcgcgaaa ccaatcaaca
    11161 tttattgctg gaaaccgccc cgaaaggctt ttcgccagac tgggtgcgct atgagaaaga
    11221 caaaggctgg cagctaaaag ccgaaaaaac attgatcagc agctacgacg ctatccgcgt
    11281 ttacatgtgg gtaggcatga tgcctgacag cgatccgcaa aaagcgcgga tgctcaaccg
    11341 gtttaaaccg atggcgacat tcactgagaa aaacggttat ccgccggaaa aagtggatgt
    11401 ggctacgggg aaagcgcagg gtaaaggacc ggtcggtttt tctgccgcca tgctgccttt
    11461 tttacaaaac cgcgatgcgc aggcagttca gcgccagcgc gtggccgata actttcccgg
    11521 cagcgatacc tattacaact atgtgctgac cctgtttgga caaggctggg atcaacaccg
    11581 tttccgcttc tcgacaaaag gtgagttatt acctgactgg ggccaggaat gcgcaaattc
    11641 acactaaaca tattcacgct ttccctcggt ctggccgtca tgccgatggt cgaggcagca
    11701 ccaaccatca gcagaccctg cggatggaca gcggcaacac taatgccgtg cgcgggctgg
    11761 cgaatattta ccgccagcag tcgccagaaa aagctgaagc gtttatcgcc tcgctctctg
    11821 ccagccagcg gcgcagcatt gatgatatcg aacgcagcct gcaaaacgac cgtctggcac
    11881 agcaggcaga ggtactggaa aaccagggca aatgggcgca ggcggcagca cttcagcggc
    11941 aacgactggc gctgggcccc ggcagcgtat ggattactta ccgactttcg caggatctct
    12001 ggcaggccgg acaacgcagc caggccgata cgttaatgcg caatctggcg cagcagaagc
    12061 cgaacaaccc ggagcaggtt tacgcttacg ggctgtacct ctcaggtcac aaccaggaca
    12121 gagcggcgct ggcgcatatc aacagcctgc cgcgcgcgca gtggaacagc aatattcagg
    12181 agctggttaa tcgactgcaa agcgatcagg tgctggaaac cgctaatcgc ctgcgagaaa
    12241 gcggcaaaga ggctgaagcg gaagcgatgc tgcgtcagca accaccttcc acgcgtattg
    12301 acctcacgct ggctgactgg gcgtaacaac gacgtgatta caccgccgcc cgcactgcat
    12361 atcagaatgt cctgacgcgg gagccaacta acgccgatgc cattcttggt ctgacggaag
    12421 tggatattgc tgccggtgac aaagcggcgg cacgtagcca gctggcgaaa ctgcctgcca
    12481 ccgataacac ctcgctgaac actcagcggc gcgtggcgct ggcacaggcg cagcttggag
    12541 ataccgcagc ggcgcagcag acgtttaata agttgatccc gcaggcaaaa tctcagccac
    12601 cgtcgatgga aagcgcgatg gtactgcgcg acggtgcgaa gtttgaagcg caggcgggcg
    12661 atccaaagca ggcgctggaa acctacaaag acgccatggt cgcatccggt gtgactacga
    12721 cgcgtccgca ggataacgac acctttaccc gactgacccg taacgacgag aaagatgact
    12781 ggctgaaacg cggcgtgcgc agcgatgcgg cggacctcta tcgccagcag gatcttaacg
    12841 tcacccttga gcacgattac tggggttcga gcggcaccgg tggttactcc gatctgaaag
    12901 cgcacactac catgttgcag gtggatgcgc cgtattctga cgggcggatg ttctttcgca
    12961 gtgatttcgt caatatgaac gtcggcagtt tctccactaa tgccgatggc aaatgggatg
    13021 acaactgggg cacctgtaca ttacaggact gtagcggcaa ccgcagccag tcggattccg
    13081 gtgccagcgt ggcggtcggc tggcgaaatg acgtctggag ctgggatatc ggtaccacgc
    13141 cgatgggctt caacgtggtg gatgtggtcg gcggcatcag ttacagcgat gatatcgggc
    13201 cgctgggtta caccgttaac gcccaccgtc ggcccatctc cagttctttg ctggcctttg
    13261 gtgggcaaaa agactccccg agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg
    13321 gtgtggggct aagtctgagc tacgataaag gtgaagtaaa cggcgtctgg gcatcgctta
    13381 gtggcgacca gttaaccggt aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg
    13441 gctattacta taaggtcatt aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca
    13501 tgatctggca ttacgacaaa gatctgagtg gctactcact cggtcagggc ggttactaca
    13561 gtccgcagga atacctgtcg tttgccatac cggtgatgtg gcgggagcgc acggaaaact
    13621 ggtcgtggga gctgggggcg tctggctcgt ggtcgcattc acgcaccaaa accatgccgc
    13681 gttatccgct gatgaatctg atcccgaccg actggcagga agaagctgcg cggcaatcca
    13741 acgatggcgg cagcagtcag ggcttcggct acacggcgcg ggcattactt gaacgacgtg
    13801 ttacttccaa ctggtttgtt ggtacggcaa ttgatatcca gcaggcgaaa gattacgcac
    13861 ccagccattt cctgctctac gtacgttatt ccgccgccgg atggcagggt gacatggatt
    13921 taccgccgca gccgctgata ccttacgccg actggtaagt tttcagatag cgcctctctt
    13981 aatgccgctg cgatcgggta tactcgggcg gcaatctggg atttccgggg ggagacaatt
    14041 tgcgcgtaag tcgctcgtta acaatcaagc agatggcaat ggtggcagcc gttgtcctgg
    14101 tgttcgtttt tattttttgc accgttttgc tgttccatct ggtccagcag aatcgctata
    14161 acacggctac gcaactggaa agcattgctc gctctgtccg cgaaccctta tcttcagcta
    14221 ttttgaaagg cgatattccc gaagcggaag ctattcttgc cagcattaaa ccggcaggcg
    14281 tggtcagccg tgccgatgta gtgctgccta accagttcca ggcgctgcgt aaaagtttta
    14341 ttccagagcg tccggtgccg gtaatggtta ctcgcctgtt tgagctaccg gttcaaatct
    14401 cgctgggcgt ctactcgctc gaacgtccgg caaatccgca gccaatagcc tatctggtgc
    14461 tacaggcgga ttccttccgt atgtataagt tcgtgatgag cactctctca acgttagtga
    14521 ccatttactt acttttgtcg cttatcctga ccgtcgccat cagctggtgc attaaccgcc
    14581 tgattttgca tccgttacgc aatattgctc gcgaacttaa cgccatccca gcccaggagc
    14641 ttgttggtca ccaactggca ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg
    14701 tgcgcagtta caacctcaac cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa
    14761 atgcgatgcg cttcccggtg tcggatttgc cgaacaaagc cttgctgatg gagatgctgg
    14821 agcaggttgt cgcgcgtaaa caaaccaccg cgctgatgat catcacctgt gaaaccctgc
    14881 gtgatactgc gggcgtgctg aaagaggcgc aacgagaaat tctgctgctg acgctggtgg
    14941 aaaaactcaa atcggtactg tcgccacgta tgatcctcgc gcagattagc ggttatgact
    15001 ttgctgtcat tgccaacggt gtacaggaac cgtggcacgc aatcactttg ggtcagcaag
    15061 tactcactat catgagcgag cgcctgccga ttgaacgtat tcaactccgt ccgcactgta
    15121 gcattggcgt ggcgatgttc tacggcgatc tcaccgccga acagctttac agtcgcgcta
    15181 tttctgcggc atttaccgct cgccataaag gcaagaatca gattcagttc tttgatccgc
    15241 agcagatgga agcagcccag aagcggttga cggaagagag cgatatcctt aatgcactgg
    15301 aaaatcatca gtttgcaatt tggttacagc cacaggtcga gatgaccagc ggtaaactgg
    15361 tcagtgcgga agtgttactg cgtatccagc aaccggatgg cagttgggac ctgccggatg
    15421 gcttaatcga tcgcattgag tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg
    15481 aagagtcctg tcgattgctt gcagcctggc aagagcgcgg cattatgctg cccttgtcgg
    15541 taaacttctc tgcgctgcaa ctgatgcacc cgaatatggt ggcggatatg ctggaactgt
    15601 taacccgcta tcgcattcag ccgggaacac tgattctgga agtgacagaa agccgacgta
    15661 ttgacgaccc tcatgctgcg gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg
    15721 tggcgctgga tgatttcggc atgggctacg cagggctgcg tcagctgcag catatgaaat
    15781 cgttgccaat cgacgtactg aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata
    15841 gcagcatgat tgctgcaatt atcatgctgg cgcagagcct gaacttacaa atgattgccg
    15901 aaggcgtgga gactgaagca caacgagact ggctggcaaa agcgggcgtt ggtattgccc
    15961 agggcttcct ttttgctcgc ccactcccta ttgaaatctt cgaagagagt tacctggaag
    16021 aaaagtagct accccaaact gattacaaaa ctttaaaaag tgctggtttg tgcgagccag
    16081 ctcaaacttt ttaacctttt tgtttcaatt atgatccagg tacatttctg tgatgttgtc
    16141 tgggtgttat tttaaggccg caggtacccc cccataacct tacaagacct gtggttttac
    16201 taaaggacac cctatgaaaa cctctctgtt taaaagcctt tactttcagg tcctgacagc
    16261 gatagccatt ggtattctcc ttggccattt ctatcctgaa ataggcgagc aaatgaaacc
    16321 gcttggcgac ggcttcgtta agctcattaa gatgatcatc gctcctgtca tcttttgtac
    16381 cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt
    16441 cgcactgctt tactttgaaa ttgtcagtac catcgcgctg attattggtc ttatcatcgt
    16501 taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc
    16561 ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt gtcgccttca ttatggatgt
    16621 catcccggcg agcgtcattg gcgcatttgc cagcggtaac attctgcagg tgctgctgtt
    16681 tgccgtactg tttggttttg cgctccaccg tctgggcagc aaaggccaac tgatttttaa
    16741 cgtgatcgaa agtttctcgc aggtcatctt cggcatcatc aatatgatca tgcgtctggc
    16801 acctattggt gcgttcgggg caatggcgtt taccatcggt aaatacggcg tcggcacact
    16861 ggtgcaactg gggcagctga ttatctgttt ctacattacc tgtatcctgt ttgtggtgct
    16921 ggtattgggt tcaatcgcta aagcgactgg tttcagtatc ttcaaattta tccgctacat
    16981 ccgtgaagaa ctgctgattg tactggggac ttcatcttcc gagtcggcgc tgccgcgtat
    17041 gctcgacaag atggagaaac tcggctgccg taaatcggtg gtggggctgg ttatcccgac
    17101 aggctactcg tttaaccttg atggcacatc gatatacctg acaatggcgg cggtgtttat
    17161 cgcccaggcc actaacagtc agatggatat cgtccaccaa atcacgctgt taatcgtgtt
    17221 gctgctttct tctaaagggg cggcaggggt aacgggtagt ggctttatcg tgctggcggc
    17281 gacgctctct gcggtgggcc atttgccggt agcgggtctg gcgctgatcc tcggtatcga
    17341 ccgctttatg tcagaagctc gtgcgctaac taacctggtc ggtaacggcg tagcgaccat
    17401 tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa ctggacgatg tgctgaataa
    17461 tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa tctcaccact tatgcccgca
    17521 ctcccttccc cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga acttaccacc
    17581 tgggtgagtt caggtttatt tcacttcttc cgtgacattt tcatgttctt gcggtctaac
    17641 acgaagtgtt tttaacgtca tttagactga ccgacaacgg gcagtttttt tattaccagg
    17701 atagttgatc aggggttcac atgcagggca taaaaattcg acttttagcg ggcggtttgc
    17761 tgatgatggc cactgctggc tatgtgcagg cagatgcgct ccagcctgat ccagcatggc
    17821 aacaggggac gctttccaac ggtttacagt ggcaagtgct gaccaccccc cagcgtccca
    17881 gcgatcgtgt tgaaattcgc ctgctggtta ataccggttc gctcgccgaa agtacacaac
    17941 agagcggtta cagtcacgcc atccctcgta ttgcgctaac gcaaagcggt ggccttgacg
    18001 cagcacaggc gcgttcattg tggcagcagg ggatcgaccc taaacgcccg atgccgccgg
    18061 taattgtctc ttatgacacc acgctgttta atctgagttt gcccaataac cgtaacgatt
    18121 tgctgaaaga agcgctctct tatctggcaa atgccactgg caaattgacc atcacaccag
    18181 aaaccatcaa ccacgcgctg caaagtcagg acatggtggc aacctggcct gccgatacta
    18241 aagagggctg gtggcgctat cgtctgaaag ggtcaacctt gttaggtcac gatcctgccg
    18301 atccgctgaa acaacccgtt gaagcggaaa aaattaaaga tttctatcag aaatggtaca
    18361 ccccggatgc aatgacgcta ctggtggtgg gaaacgtgga tgcgcgctcg gttgtcgacc
    18421 aaatcaacaa aacgtttggc gaactgaaag gcaaacgtga aacgccagct ccggtgccga
    18481 cgctttctcc gctgcgtgcg gaagcggtga gtattatgac tgacgcggtg cgtcaggacc
    18541 ggttatctat catgtgggat acgccgtggc agccgattcg tgaatcagcc gcactgctgc
    18601 gctactggcg tgcggacctg gcccgcgagg cgctgttctg gcatgttcag caagcgttaa
    18661 gcgccagtga cagcaaagac atcggtcttg gatttgactg ccgtgtgctg tatctgcgtg
    18721 cgcagtgtgc catcaacatc gaatcaccaa acgacaagct gaacagcaac cttaatctgg
    18781 tggcgcgtga actggcgaag gttcgcgata aaggtctgcc ggaagaagag ttcaatgcgt
    18841 tagtggcgca aaagaaactg gagctgcaga aactgtttgc cgcctatgca cgggctgata
    18901 ccgatattct gatgggccag cggatgcgtt cgttgcaaaa tcaggtcgtc gatatcgcgc
    18961 cggagcagta tcagaaactg cggcaggatt tccttaacag cctgactgtg gagatgttaa
    19021 atcaggatct gcgtcagcag ttgtcgaatg atatggcgtt aatactgctg cagccgaaag
    19081 gcgagccgga atttaacatg aaagcgttgc aggcggcctg ggatcaaatc atggccccat
    19141 ctaccgccgc tgcgaccacc tctgtcgcca cggatgacgt acatcctgaa gtgacggata
    19201 ttccacctgc acagtaatgc ctgatggtga tgagttagca caaccatcag gtatttatgg
    19261 atgagctgga tagaggggta acactttatc cagccttttg catatgctgc gtttacgctg
    19321 gcatcgcctc acgcgggata atcgcgccgc gatactgaat aacggtactt gcggtcaggt
    19381 gcccgcgttt cgccgcgttt tccgcactgc cgcccgtcaa acgtaccgcc agataaccgg
    19441 cgctgaaaga gtcgccagct gcggtggtat cgatcacttt ttctttcggc agtttcaccg
    19501 ccggaacatc aactaacgct tcaccagcaa tggacaccag gcaagaatcc gccccgcgtt
    19561 tcaccaccac ttctttcacg cccgcgttat gggtgcgcgc aatgacgtct tccaccggct
    19621 gttgacccca cagcgcgtct tcgtcgtcca gcgtcaggaa ggcgatatcc gtgcattcca
    19681 gcatttgctg gtacacctgc tgtgtctctt ctttgctggc ccacaggcgc ggacgatagt
    19741 tattgtcgaa aatcactttt ccgcctttgg cgcggcattc gcgcagcagg gaaagcagct
    19801 tttcgcggct ggtcgggctt aagatcgcca ggctaatccc gctcaggtag agataatcga
    19861 aattcgccag ctcttcgcaa atcgccgcag actgctcgct cgccagccag aatttggcgg
    19921 cggcttcgtt acgccagtag tagaacgtac gttcgccggt gctgtcggtt tcaatgtagt
    19981 aaaggcccgg aagacggttt tccatccgtt gggtcaggga agtatcaacg ttctcgccgt
    20041 gccaggcgtc cagcatctgc tggctaaaac tgtccgttcc cagcgccgtt acgtaatgaa
    20101 cggttaatgc cgcaggatcg acctgacggg cgatatagac ggaagtgttc agggtatcgc
    20161 cgccgaaacc gcgcttaacg tccgcgcctt tctcggaaag ctcaatcatg cattcgccaa
    20221 tcacggcaat ctttttggac atagtcgtga acctgatctg taaaaaatta gcgttagttt
    20281 gcgctgtggt ggtttgctgg tcaaccatat taaaacagtg ttccattatt tttttgagcc
    20341 agaacgtatt cctgaaagat tccgttgtgg agtgaggaaa tttaccccgg ccaggcggta
    20401 aagttctgcc cttacgcgcc gataatcttt gtcgagtccg ggcagcatca cttttaaaca
    20461 caggacatct ttgatgataa ggcaggttat ccagcgaata agcaaccctg aagcaagcat
    20521 cgagagcttg caggaacggc gtttttggtt gcagtgtgag cgtgcttaca cctggcagcc
    20581 gatctatcaa acatgcgggc ggttaatggc cgtggagcta ttaacggtgg tcacgcatcc
    20641 cttgaaccct tcgcaacgcc tgccgccgga tcgctatttt actgaaatca ccgtcagcca
    20701 tcggatggag gttgtgaaag agcagattga tttgctggcg caaaaagccg acttctttgt
    20761 agagcacggc ctgctggcat cggtcaatat tgatggccct acgctcatcg ccctgcgtca
    20821 gcaaccaaaa atcctgcgcc agattgagcg tcttccctgg ctgcgtttcg aactggtgga
    20881 gcatatccgt ctgccgaaag attcaacctt tgcctcgatg tgtgaatttg gcccgctgtg
    20941 gctggatgat tttggtaccg ggatggcaaa tttctctgca ctaagtgaag tgcgttatga
    21001 ctacatcaaa atcgcgcgag aactgtttgt gatgctgcgt cagtcgccgg aaggccgcac
    21061 actcttttct cagcttttac atctaatgaa tcgctattgt cgcggggtga ttgtcgaggg
    21121 cgtagaaaca ccggaagagt ggcgtgatgt tcagaactcg cccgcattcg ccgcacaagg
    21181 ctggtttctt tcacgcccgg caccgatgga aacgctgaat acggcggttc tggcgctata
    21241 agctgcctca ttttccgccg ggctggacta tctttaggac tggcacagga agaatatgag
    21301 gaagcgaacg atgagcaagg caggcaaaat aaccgctgcg atttcagggg ctttcttgtt
    21361 gttgattgtc gtggcgatca ttttgattgc aacttttgac tggaatcgac tcaaaccgac
    21421 catcaaccag aaagtctctg cggagttgaa tcgtccgttc gctatccgtg gcaatctggg
    21481 cgtggtgtgg gagcggcaaa aacaagaaac tggctggcgc agctgggtgc cgtggcctca
    21541 tgtacatgca gaagacatca ttcttggtaa tccaccggat attcccgaag tcacgatggt
    21601 gcatttgccg cgagtagagg caacgctggc accgctggcg ctgctgacca aaacggtctg
    21661 gctgccgtgg atcaagctcg aaaagcccga cgcgcgcctg attcgcctct ctgaaaagaa
    21721 caataactgg acgtttaatc ttgccaacga tgacaacaaa gacgcgaatg caaagccgtc
    21781 ggcatggtcg tttcggctgg ataatattct tttcgatcaa gggcggatcg ccattgatga
    21841 caaagtaagc aaagcggatc tggaaatttt tgttgatccg ttaggcaagc cgctgccatt
    21901 cagcgaagtt actggatcga aaggtaaagc ggataaagaa aaggtgggcg attacatttt
    21961 tggcctgaag gcgcagggac gttataacgg tgaaccgctc acgggtacgg gaaaaatagg
    22021 cggtatgctg gcgctgcgtg gcgaagggac gccgtttccg gtacaggctg atttccgttc
    22081 aggtaatacc cgtgttgctt ttgatggcgt cgtgaatgac ccaatgaaga tgggcggtgt
    22141 cgatttacgg cttaaatttt ctggcgattc actgggtgat ctctatgaac tgacgggcgt
    22201 tctgctgccc gataccccgc cgtttgaaac ggatggtcgg ctggtagcga aaatcgacac
    22261 tgaaaaatcg tcggtctttg attatcgcgg ttttaatggg cgaattggcg atagcgatat
    22321 ccacggttct ctggtctaca ccaccggaaa gccacgacca aaactggaag gtgatgtcga
    22381 gtcgcggcaa ttgcggctgg cggacctggg accgttgatt ggcgttgatt ccgggaaagg
    22441 ggcagaaaag tcgaaacggt ctgaacagaa gaagggcgaa aaaagcgttc agcctgcggg
    22501 caaagtgctg ccttatgacc gcttcgaaac cgataaatgg gacgttatgg atgccgatgt
    22561 tcgcttcaaa gggcggcgca ttgagcatgg cagtagcctg ccgattagcg atctttctac
    22621 tcatatcatc ctcaaaaatg ctgacctgcg cctgcaaccg ctgaaatttg gcatggcggg
    22681 cggcagcatt gcggcgaata ttcatctgga aggcgataaa aagccgatgc aggggcgggc
    22741 agatattcag gctcgtcgac tgaaactgaa agaactgatg cccgatgtgg aactgatgca
    22801 gaagacgctg ggggaaatga acggtgacgc ggaactacgc ggtagcggta actcggtggc
    22861 ggcgctttta ggcaacagta acggcaacct gaaactgttg atgaatgacg ggctggtgag
    22921 ccgcaacctg atggagattg ttgggctgaa tgtcggcaac tacattgtcg gtgcgatatt
    22981 tggtgacgat gaggtgcggg tgaactgcgc ggcggcgaat ctgaatattg ccaacggcgt
    23041 ggcgcgcccg cagatttttg ctttcgatac tgagaacgcg ttgattaatg ttaccggcac
    23101 ggcaagtttt gcttcggaac agctggattt gactattgat ccggagagta aagggattcg
    23161 gattatcaca ctgcgttcgc cgctgtatgt gcgtgggacg tttaaaaatc cgcaggctgg
    23221 ggtgaaagcc ggaccgctga ttgcccgtgg tgctgttgct gcggcactgg caacgctggt
    23281 aacaccggcg gcggcgttac tggcactgat ctcaccttcc gaaggggagg ctaatcagtg
    23341 tcggacgata ttgtcgcaga tgaagaagtg atgataattg ccggatgtct gacatccggc
    23401 aattaaacag atttagattt ttggcaaagt gtgctttgtt catgccggat gcggcgtgaa
    23461 cgccttatcc ggcctacaaa tccttgtgaa ttcaatatat tgtatggaaa ttcagtcctg
    23521 ataagcgtag cgcatcaggc ttttcacact tattacaacg actgatgtcg cgtctcatgg
    23581 gtcagcagca gggcgattaa cgtcaagcca gccatcgccg ccagatataa ccccaccgca
    23641 cctaacccgt agttagcctg caaccaggct gcgatatacg gcgcaacgga agccccgaga
    23701 atcgacgata cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt cggaaacagc
    23761 tctggtaaca gcgcacccat cggcccgaag gtcagcccca tcagacttaa ccccagcagc
    23821 aggaaggcaa aaaccagaat cgggttgcca gaaccgagca gcgggttaaa ggcgaacagc
    23881 gcgaacagga tgatcagcgt ggtgatgatc accatgcttt tacggcgacc aaaggcatca
    23941 gccagtaatc cggcgactgg caccatcacg ccaaaaccaa taactgccat catcagcatc
    24001 cacaacactt cgttacgcgg caggccaagc ccaactggcg cggcggcggt actgaaggtc
    24061 atagagtaga ccgtcatgat gtaaaacagc gtataggttg ccagcataat gaacgtaccc
    24121 agtacggtta cgcgaacatg tttggtcagc agcgtaccca gcgggatctt cacctgtttt
    24181 ttcgctttag cgactttctc aaacaccggc gactcatgca gcgacacgcg aacatacagg
    24241 ccgataatga ccagcaccgc cgagaagata aatggcacac gccagcccca gctcataaac
    24301 tgctcatcgg tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca
    24361 atcggtgcgc ctagctgtgg gaaggagcca tacagtgcac gtttgcgcgg tggggcgttt
    24421 tcagtcgcca gcagcgccgc accgccccat tcaccgccta agcccagacc ctgaccaaat
    24481 cgagccagcg ccagcagcag tggggcgaaa ataccaatcg tggcatagcc cggcagcaga
    24541 ccaatcacca cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc
    24601 ccaacgcgat cgccaaaatg accaaaaacg gcagagccaa tggggcgcgc gacgaaggcg
    24661 atggcgaagg tggcgagcga ctgtagcgtt gctgccgttg gatcgccctg cgggaagaag
    24721 atatgcggaa acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg
    24781 gccgtgccaa tgagagaggc gacaaggact ttattacgcg agttgatcgg cgtgtattct
    24841 tgttcgtggt cgagtgttgt ggctgttgct tgcataatct tttcttattt tttggctaac
    24901 gaatagccat attacgcaca gcaaaagtga tatttcaatg tgtaacaaat cgcggaaatg
    24961 gtcaaaaacc aggcaaaaag gggataatgt ttaggccagt gatttttctt ctatgaaatg
    25021 cttcacataa ttttataaat agtggataaa actggtttca ggttaaataa aagttaccaa
    25081 ctggatttat atgctggggt tatgagttgg agtgaaattg tttgctccca aatagatggc
    25141 tgaagaggct ctggggaaga gtttcaagag gttatgtgcg cattatagcc ggatgcgatg
    25201 ctggcgcatc ttatccggcc tacggggagc caggccgaat aaaatcgtat gccggataag
    25261 gcgtttacgc cgcatccggc ctattttaag gctgcgtttt ccctggcatt cgcgggtcgt
    25321 ctttatattc ggcggtggca atccacgccg cgcaaaacag cgtcaaacgg gcgaagaagt
    25381 agaaaaatgc catcagcccc agcacggaac caaatgctgc gccagagggg gatttcatca
    25441 gcgatggcag ggtgtaggtc atcacgattt taatcacttc aaaaccaata gcagccagaa
    25501 atgttccgcg aatcagggct tttttgcgcg gacggtggcg cggcaaccgc cagaagatcc
    25561 agaagaaaag cagatagttg gcgaagatgg aaatcgccag gccaatcaat cgccacgtcg
    25621 gtttcagcca ctcaatgctg ttcaggtgca gggcgctaat aatcatttgc tgcgccgaac
    25681 cggcaaccga ggtgatcgaa agcgtcacaa tcagcgcaat caacaaacca atcagtgaaa
    25741 taaaatcacg cagatatttt acccagaact tctcctgatc ttgcggcgag cgttcccaga
    25801 catcgcgcga ctgggcacga atcgcttcac gcaggttacc catccagttg atgccggaat
    25861 aaagtgccac cgccaggccg acaagcccta cagtcgtacg ctgctgaacg gcggtgttga
    25921 tggtgttttt caacgtggcg gctagtgtcg gatcgctgat gttttgcaga attttgtcga
    25981 agatatcctg tagcaacatc ggatgggagg ccagcacaaa gccccctgcg gcaaacgaca
    26041 ccatcaaaat cgggatcatc gacaaaaatg agaaataggt gatagtcgca ccaaactggt
    26101 tacccagccg atcgttaaag cgttctgtcg cgcgaataag gtgcgcaata accggttggc
    26161 gttggacttt ttcggcagtg gtggtgacgg tttccagcgc ctggctaact ttgccgcctt
    26221 tctcgctatt atccaactgc ttaatcggct cgtgctccag atcctgggtg ggacgtttga
    26281 tctcgttttc ctgcgtcatc cgctcttttg tccttctgtc gtggtcattc ttaaaaagta
    26341 tagtcggtca gtccaggtgc tctttcatta cgccagccag ccactgcata aacaggttta
    26401 cacgccggga aagctcccga cgctgcgggt aaaccagcga aagggagagc ggctcggcac
    26461 ggtagcctgg caacacttca ataagccgcc cggcacgcag ggcttcgcgc acggcaatgc
    26521 gcgggatctg aataatcccc agaccggcaa gaccggcggc gagatagttt tcgctactgt
    26581 ttaccgtcaa catgccgcca gacttaaacc actggacgcc attaacgctg gcaacctcaa
    26641 aacctaacgg atgtacaccc aggtgcggtg tataacgcac tattgcgtgt gaagtcagat
    26701 catcggggct ttgcggataa ccaaagcgcg tcagatagtg cggactggca cagttgacca
    26761 tggttagttt gccgagggga cgggcgataa cgccatcttc cggtaacgcg ccagtgcgta
    26821 tcacgcaatc aaaaccatca tgaagaatat ctaccggacg gttatggcta ctcagttcca
    26881 gctcaattcc cggatgctga tagagaaatt ccgacagacg cggcagtaac aggtttttcg
    26941 cgattcctga cgggatgtcg atgcgtaatt taccgctgat actggtggca tcctgttgaa
    27001 acagaccgtc cagttcgctg agattactca acacatcttt tgcccgttga taataggtca
    27061 tgccttctgg cgtgagtttg acccgtcgcg tagtgcgctg gagaagctgg gtgccaagtt
    27121 gatgttccag tgcctgtatc tggcgcgaaa cacttccctt tggcaaagca aagaaatccg
    27181 ccgcgcggga aaaactttcc agctccgcga ctttgatgaa caactgcatt gcgtgaattt
    27241 tatccatttc tttgaccgat tgttgttcac agcgaaacag taacgcgcat atcgcattgt
    27301 ttattgtttt ttctaacatc taatgccggg tttcacaatg ttgtcatgac gaatgtggcg
    27361 atttctcagc aggaaaaggg cggaatagct ttatgagaaa ggtgaatccc cttaaacaga
    27421 gcgtattttt cagtgattcc ctttaaagtg aatagaggac gtattggcta tttgtgctta
    27481 tttttgtctc attgccattt gttattttgg cttgtagtcg cggaatttca tttttaattg
    27541 catgcgtact aaccaccaat ataaggaaat cctcaaaatt gttttaaata gtgccctcga
    27601 catgtaattt caggcgttta gctgtaacta tttatatcgg taactatttt ctacgcgtca
    27661 tctctgtaga atggccaccg ccaaatggcg aattcttatt ttttctcgcg ggtaataccc
    27721 ttccactact taagtctttt taacgtattc aggtggagat tcagttatgc aaatagtcat
    27781 gtttgacagg cagtcaatat ttattcatgg aataaaaatc agtttacagc agcgtattcc
    27841 aggagtgagt attcaggggg tcagtcaggc agacgagtta tggcaaaagc tggaaagtta
    27901 ccctgaagcc ttagttatgc tcgatggcga tcaggatggt gagttttgct actggttgct
    27961 gcaaaaaacc gtggtgcagt ttcctgaggt taaggtgtta atcacggcga cggattgcaa
    28021 taaacggtgg ttacaggaag ttatccattt taatgtgctg gccattgtgc ctcgcgattc
    28081 aaccgtcgag acatttgcgt tggcggtaaa tagtgcggcg atggggatga tgtttctgcc
    28141 tggagactgg cggacgacgc cggaaaagga cattaaagat ctcaaatcgt taagcgcccg
    28201 ccagcgggag attttaacca tgttagcggc gggcgaatca aataaggaga tcggcagagc
    28261 gctgaatatc agtaccggaa cggtaaaagc gcatctggaa tccctgtatc gccgtctgga
    28321 agtgaaaaat cgcacccagg cggcaatgat gttaaatatc tcctcctg
//
LOCUS       DBKELA010000053        28328 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.053, whole genome shotgun sequence.
ACCESSION   DBKELA010000053 DBKELA010000000
VERSION     DBKELA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.053"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="ACUQR0_002191"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="ACUQR0_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(998..2020)
                     /gene="ytfT"
                     /locus_tag="ACUQR0_002192"
     CDS             complement(998..2020)
                     /gene="ytfT"
                     /locus_tag="ACUQR0_002192"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HHV9109216.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /gene="ytfR"
                     /locus_tag="ACUQR0_002193"
     CDS             complement(2034..3536)
                     /gene="ytfR"
                     /locus_tag="ACUQR0_002193"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HHV9109217.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="ACUQR0_002194"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="ACUQR0_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="ACUQR0_002195"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="ACUQR0_002195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HHV9109218.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="ACUQR0_002196"
     CDS             complement(5851..6192)
                     /locus_tag="ACUQR0_002196"
                     /EC_number="4.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061929 -
                     gamma-glutamylaminecyclotransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HHV9109219.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="ACUQR0_002197"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="ACUQR0_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0089705 - protein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HHV9109220.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="ACUQR0_002198"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="ACUQR0_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0089705 - protein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HHV9109221.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="ACUQR0_002199"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="ACUQR0_002199"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HHV9109222.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="ACUQR0_002200"
     CDS             12871..14214
                     /locus_tag="ACUQR0_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HHV9109223.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="ACUQR0_002201"
     CDS             complement(14276..14482)
                     /locus_tag="ACUQR0_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HHV9109224.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="ACUQR0_002202"
     CDS             14807..15361
                     /locus_tag="ACUQR0_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HHV9109225.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="ACUQR0_002203"
     CDS             complement(15424..16362)
                     /locus_tag="ACUQR0_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109226.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="ACUQR0_002204"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="ACUQR0_002204"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HHV9109227.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="ACUQR0_002205"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="ACUQR0_002205"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HHV9109228.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="ACUQR0_002206"
     CDS             complement(19573..19953)
                     /locus_tag="ACUQR0_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HHV9109229.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="ACUQR0_002207"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="ACUQR0_002207"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HHV9109230.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="ACUQR0_002208"
     CDS             21011..21976
                     /locus_tag="ACUQR0_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHV9109231.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="ACUQR0_002209"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="ACUQR0_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HHV9109232.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="ACUQR0_002210"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="ACUQR0_002210"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HHV9109233.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="ACUQR0_002211"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="ACUQR0_002211"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHV9109234.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="ACUQR0_002212"
     CDS             25350..25895
                     /locus_tag="ACUQR0_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HHV9109235.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="ACUQR0_002213"
     CDS             complement(25879..26526)
                     /locus_tag="ACUQR0_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHV9109236.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="ACUQR0_002214"
     CDS             26738..27529
                     /locus_tag="ACUQR0_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HHV9109237.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28328
                     /locus_tag="ACUQR0_002215"
     CDS             27539..>28328
                     /locus_tag="ACUQR0_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHV9109238.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQK"
BASE COUNT         6948 a         7541 c         6988 g         6851 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaag
//
LOCUS       DBKELA010000054        27534 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.054, whole genome shotgun sequence.
ACCESSION   DBKELA010000054 DBKELA010000000
VERSION     DBKELA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.054"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACUQR0_002216"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACUQR0_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HHV9109239.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYTAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACUQR0_002217"
     CDS             complement(1614..1937)
                     /locus_tag="ACUQR0_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HHV9109240.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACUQR0_002218"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACUQR0_002218"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HHV9109241.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="patZ"
                     /locus_tag="ACUQR0_002219"
     CDS             complement(3452..6112)
                     /gene="patZ"
                     /locus_tag="ACUQR0_002219"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-lysine N-acetyltransferase PatZ"
                     /protein_id="HHV9109242.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSYARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACUQR0_002220"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACUQR0_002220"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HHV9109243.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACUQR0_002221"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACUQR0_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HHV9109244.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACUQR0_002222"
     CDS             7537..8574
                     /locus_tag="ACUQR0_002222"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HHV9109245.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACUQR0_002223"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACUQR0_002223"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HHV9109246.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACUQR0_002224"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACUQR0_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HHV9109247.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACUQR0_002225"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACUQR0_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HHV9109248.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11617
                     /locus_tag="ACUQR0_002226"
                     /pseudo
     CDS             10745..11617
                     /locus_tag="ACUQR0_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACUQR0_002227"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACUQR0_002227"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HHV9109249.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACUQR0_002228"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACUQR0_002228"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HHV9109250.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACUQR0_002229"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACUQR0_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACUQR0_002230"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACUQR0_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HHV9109251.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACUQR0_002231"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACUQR0_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HHV9109252.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACUQR0_002232"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACUQR0_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HHV9109253.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACUQR0_002233"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACUQR0_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HHV9109254.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACUQR0_002234"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACUQR0_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HHV9109255.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACUQR0_002235"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACUQR0_002235"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HHV9109256.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACUQR0_002236"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACUQR0_002236"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHV9109257.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACUQR0_002237"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACUQR0_002237"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HHV9109258.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACUQR0_002238"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACUQR0_002238"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HHV9109259.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACUQR0_002239"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACUQR0_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HHV9109260.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACUQR0_002240"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACUQR0_002240"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HHV9109261.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACUQR0_002241"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACUQR0_002241"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HHV9109262.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACUQR0_002242"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACUQR0_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HHV9109263.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACUQR0_002243"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACUQR0_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HHV9109264.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27534
                     /locus_tag="ACUQR0_002244"
                     /pseudo
     CDS             26442..27534
                     /locus_tag="ACUQR0_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         6795 a         6854 c         7218 g         6667 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttata ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtagctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgt
//
LOCUS       DBKELA010000055        26589 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.055, whole genome shotgun sequence.
ACCESSION   DBKELA010000055 DBKELA010000000
VERSION     DBKELA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.055"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..133)
                     /locus_tag="ACUQR0_002245"
     CDS             complement(<1..133)
                     /locus_tag="ACUQR0_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HHV9109265.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     "
     gene            375..668
                     /locus_tag="ACUQR0_002246"
     CDS             375..668
                     /locus_tag="ACUQR0_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HHV9109266.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            740..1399
                     /locus_tag="ACUQR0_002247"
     CDS             740..1399
                     /locus_tag="ACUQR0_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HHV9109267.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1491..1937
                     /locus_tag="ACUQR0_002248"
     CDS             1491..1937
                     /locus_tag="ACUQR0_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HHV9109268.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2144..3044)
                     /gene="hlyE"
                     /locus_tag="ACUQR0_002249"
                     /pseudo
     CDS             complement(2144..3044)
                     /gene="hlyE"
                     /locus_tag="ACUQR0_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0090729 - toxin activity [Evidence IEA];
                     GO_process: GO:0044179 - hemolysis in another organism
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3417..3836
                     /locus_tag="ACUQR0_002250"
     CDS             3417..3836
                     /locus_tag="ACUQR0_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HHV9109269.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3836..5104
                     /gene="umuC"
                     /locus_tag="ACUQR0_002251"
     CDS             3836..5104
                     /gene="umuC"
                     /locus_tag="ACUQR0_002251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HHV9109270.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5150..5680)
                     /gene="dsbB"
                     /locus_tag="ACUQR0_002252"
     CDS             complement(5150..5680)
                     /gene="dsbB"
                     /locus_tag="ACUQR0_002252"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HHV9109271.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5826..7367)
                     /gene="nhaB"
                     /locus_tag="ACUQR0_002253"
     CDS             complement(5826..7367)
                     /gene="nhaB"
                     /locus_tag="ACUQR0_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HHV9109272.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7588..8307
                     /gene="fadR"
                     /locus_tag="ACUQR0_002254"
     CDS             7588..8307
                     /gene="fadR"
                     /locus_tag="ACUQR0_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HHV9109273.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8359..9891)
                     /locus_tag="ACUQR0_002255"
     CDS             complement(8359..9891)
                     /locus_tag="ACUQR0_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HHV9109274.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10221..11519
                     /gene="dadA"
                     /locus_tag="ACUQR0_002256"
     CDS             10221..11519
                     /gene="dadA"
                     /locus_tag="ACUQR0_002256"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HHV9109275.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11529..12599
                     /gene="dadX"
                     /locus_tag="ACUQR0_002257"
     CDS             11529..12599
                     /gene="dadX"
                     /locus_tag="ACUQR0_002257"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HHV9109276.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12767..14181)
                     /locus_tag="ACUQR0_002258"
                     /pseudo
     CDS             complement(12767..14181)
                     /locus_tag="ACUQR0_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14720..14857
                     /locus_tag="ACUQR0_002259"
                     /pseudo
     CDS             14720..14857
                     /locus_tag="ACUQR0_002259"
                     /inference="COORDINATES: protein motif:HMM:NF049075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14792..16528)
                     /locus_tag="ACUQR0_002260"
     CDS             complement(14792..16528)
                     /locus_tag="ACUQR0_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HHV9109277.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16623..17537)
                     /gene="ldcA"
                     /locus_tag="ACUQR0_002261"
     CDS             complement(16623..17537)
                     /gene="ldcA"
                     /locus_tag="ACUQR0_002261"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HHV9109278.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17637..18248
                     /gene="emtA"
                     /locus_tag="ACUQR0_002262"
     CDS             17637..18248
                     /gene="emtA"
                     /locus_tag="ACUQR0_002262"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HHV9109279.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18250..18984)
                     /gene="ycgR"
                     /locus_tag="ACUQR0_002263"
     CDS             complement(18250..18984)
                     /gene="ycgR"
                     /locus_tag="ACUQR0_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0071945 - regulation of bacterial-type
                     flagellum-dependent cell motility by regulation of motor
                     speed [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein YcgR"
                     /protein_id="HHV9109280.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19185..19439
                     /locus_tag="ACUQR0_002264"
     CDS             19185..19439
                     /locus_tag="ACUQR0_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HHV9109281.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19489..21443)
                     /locus_tag="ACUQR0_002265"
                     /pseudo
     CDS             complement(19489..21443)
                     /locus_tag="ACUQR0_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(21469..22323)
                     /gene="modD"
                     /locus_tag="ACUQR0_002266"
     CDS             complement(21469..22323)
                     /gene="modD"
                     /locus_tag="ACUQR0_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HHV9109282.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22323..23132)
                     /locus_tag="ACUQR0_002267"
     CDS             complement(22323..23132)
                     /locus_tag="ACUQR0_002267"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHV9109283.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23142..23900)
                     /locus_tag="ACUQR0_002268"
     CDS             complement(23142..23900)
                     /locus_tag="ACUQR0_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9109284.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23897..24877)
                     /locus_tag="ACUQR0_002269"
     CDS             complement(23897..24877)
                     /locus_tag="ACUQR0_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HHV9109285.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24877..25899)
                     /locus_tag="ACUQR0_002270"
     CDS             complement(24877..25899)
                     /locus_tag="ACUQR0_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HHV9109286.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26248..>26589
                     /locus_tag="ACUQR0_002271"
     CDS             26248..>26589
                     /locus_tag="ACUQR0_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109287.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6689 a         6477 c         6859 g         6564 t
ORIGIN      
        1 gtgtccatat tacgtgggta ggatcactat ggcctttgct gaactcaaca ttgacattct
       61 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
      121 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
      181 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
      241 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
      301 agctggctgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
      361 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
      421 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
      481 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
      541 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
      601 cacccgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
      661 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
      721 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
      781 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
      841 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
      901 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
      961 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     1021 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     1081 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     1141 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     1201 cgaaaaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     1261 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     1321 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     1381 gacaactcgc gttttgtaat actttttgcc gccagaaagc ggcggcaaaa cttgcatcgc
     1441 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     1501 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     1561 gtgatggttg cggtcagtgt tgcttgcata aactgatgga tgaagacacc gacgaaatct
     1621 acttcactaa cgtcgcctgt cgccagctca atattaaaac ctgtcaatgt cggaactacg
     1681 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     1741 tcgaatggct gccaatgacc tgcgcttatc ggttgctggc ggaaggtaaa gatttacctg
     1801 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     1861 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     1921 ctgactgggc gcagtgataa atttaataga tgctgatggg ggctttctta atatacgtct
     1981 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     2041 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     2101 gataaacctt ataccttgag tacatggaga gaataatcgc ttatcagact tcaggtactt
     2161 caaagagtgt ctttttaccg tgtcttttct gatactcatt acaggtgtta atcatttttt
     2221 tggccgctgc ttttagcaaa gaaagcatta aatcatcata atcaacatag aatctggttg
     2281 tttcagtttc cgtttttatc tccccgatgg cggctatttc ggtggttaat ttcaatttgg
     2341 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     2401 gcacagattt taacttgttc ttcaattctg gaatcagttt cccttcaact acgcccgcag
     2461 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacatcggct gcggcaccgg
     2521 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     2581 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     2641 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     2701 ccttaatgag gggcggatgc tttcttctca ttgtactcat caaatagcaa aatatacgct
     2761 gcgagcaatt gcgtcgcaac accacaccat tcatacactg tttgggttgc ttcaaaatac
     2821 ttatcctggc tatccataag taaggtttta atatcgccga ctaaaacgga ggctgcctgt
     2881 gaatactcct gtttaaagcg acttaactct tttatggttt catcaaaggt ctgccagggg
     2941 atgacctgat cgagatattt attataaaga tctaatgctc catctgcggt ttcgattgcg
     3001 tttttaacta cttctaccgt tttatctgca acgatttcag tcataatcat tcgcctcttt
     3061 aaatatataa attgtaatga aactcctgtt ttacaactat taataaattt tacttcatct
     3121 aattcatagt tagccgggcg ggatgcgtca atgtctttat ttctattaat atgataaata
     3181 tcaaacaatg tttaatgtca ttatggcgaa tgcttctatt ctattttttt gccgggtgat
     3241 atttttcatt tctgctggat gagcgtcgtc gccagaaggc cacgtgagca caggataaga
     3301 gaacgaaaaa tcagcagtct atgcagcgac aaatattgat agcctgaatc agtattgatc
     3361 tgctggcaag aacagaccac tgtatataaa aacagtataa cttcaggcag attattatgt
     3421 tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt tccgctattt agcgatcttg
     3481 ttcagtgtgg ctttccttca ccggcagcag attacgttga acagcgcatc gatctgaatc
     3541 aactgttgat ccagcatccc agcgcgactt acttcgtcaa agcaagtggt gattctatga
     3601 ttgatggtgg aattagtgac ggtgatttac tgattgtcga tagcgctatt accgccagcc
     3661 atggtgatat tgtcatcgct gctgttgacg gcgagtttac ggtgaaaaaa ttgcaactac
     3721 gcccgacggt acagcttatt cccatgaaca gtgcgtactc gcccattacc atcagtagcg
     3781 aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt taaggcgatg cgctgatgtt
     3841 tgccctctgt gatgtaaacg cgttttatgc cagctgtgag acggtgtttc gccctgattt
     3901 atggggtaaa ccggtggttg tgctatcgaa taatgacggt tgcgttatcg cccgaaacgc
     3961 tgaggcaaag gcgctgggcg ttaaaatggg cgatccctgg ttcaaacaaa aagatctgtt
     4021 tcgtcgctgt ggtgtggttt gctttagcag caattatgag ctttacgcag acatgagcaa
     4081 tcgggtgatg tcgacgctgg aagaactatc gccccgcgtc gagatttaca gtattgatga
     4141 agcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat ctgactgatt ttggcagaga
     4201 aattcgcgca acggtgctac aacgtaccca tcttactgtt ggtgtgggga tcgcccagac
     4261 caaaacgttg gctaagcttg ccaatcatgc ggcaaaaaaa tggcagcggc agacgggtgg
     4321 ggtggtggat ttatcaaatc tggaacgcca gcgtaaatta atgtctgctc tccccgtgga
     4381 tgacgtctgg gggattggac ggcggatcag caaaaaactg gacgcgatgg ggatcaaaac
     4441 cgttctcgat ttggcggata cagatatccg gtttatccgt aaacatttta atgtcatgct
     4501 cgaaagaacg gtgcgtgaac tgcgcggcga accctgtttg caactggaag agtttgcacc
     4561 gacgaagcag gaaattatct gttcccgctc gtttggtgaa cgcatcacgg attatacgtc
     4621 gatgcggcag gccatttgta gttacgctgc ccgggcggcg gaaaaacttc gcagtgagca
     4681 tcaatattgt cggtttatct ccacatttat taagacgtca ccatttgcgc tcaatgaacc
     4741 ttattacggc aatagcgcgt cggtaaaact gctgacgccc actcaggaca gcagggatat
     4801 cattaacgct gctacgcgat ctctggatgc catctggcaa gcgggccatc gctaccaaaa
     4861 agcgggcgtg atgctggggg atttcttcag tcagggagtc gcgcagctca atttattcga
     4921 tgacaacgca ccgcgccccg ggagtgagca attgatggcg gtaatggata ctctgaatgc
     4981 taaagagggc agaggaacac tctattttgc cgggcagggg atccaacaac aatggcagat
     5041 gaagcgagct atgctttcac cacgttatac aacgcgaagt tctgatttac tgcgggtcaa
     5101 ataaatatag cggcaggaaa aaagcgctcc cgcaggagcg ccgaatggat tagcgaccga
     5161 acagatcacg ttttttcgct ttaaacggct gggaaatcac caccagcacc gcgacaatca
     5221 ggtaagcgat aaaaataccg agcagccact gcggcatttc catacctaaa aaatcccact
     5281 gacgctcggc gcaatcgcca gaggcgacaa acacttgcgg cacccattta tccagcggca
     5341 gccattccgg gaaacgaacc ataaaatcac aggtggcaaa tggcgaagga tagagctgaa
     5401 gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc actatacaac cagataacca
     5461 tcgctacata acgcagcgga gttttcgggg cgatcgcgcc aatcagcgca gcacccagaa
     5521 cgccgaataa cgcgcagcgt tcataaatac agagcacgca aggtttcagt aacatcacat
     5581 gctggaacca cagcgccgtc agttccagtg ccagagcagt aaacgccatc aacagccacg
     5641 cgccccggcc ttgtgaacat tggttcaaaa atcgcaacat aatcatttcc ctgcaatatg
     5701 catagagtgc gcagtttaaa ccaattcatt cgctgcgcca ccaggaggca gcaaaaatag
     5761 gacgcaatag gacaattatc gggcgaaaag gcaaaccggg catttatatg cccggtaagt
     5821 tgttatcaaa gcgttgctat ccagcccatt tgcataaacc attcggttac aggggcaagc
     5881 gtaaactcga cgcagagcaa gccgacgagt gtcagtacga gggtgtaagg cagggccatc
     5941 cacaccatgc ggccataaga gaggcgaatc aatggcgcga gtgcagaggt cagcaggaac
     6001 aggaacgcag cctgaccgtt cggcgtagcg acagagggca gattggtacc ggtattaatg
     6061 gcgaccgcca gcagctcgta ttgcttcaac gtgatagcgc cactttccat tgccgctttc
     6121 gcttcgttga tataaatcgt ccccacgaag acgttatccg aaatggatga cagcagaccg
     6181 ttgaaaatat agaacagcga cagctgagca tgttcagatg cctgcaacac aaactgaata
     6241 atcggcgaaa acagttgttg gtcgataatc accgcgacta ccgagaaaaa caccgtcaac
     6301 agtgcggtga atggcagaga ttcggtgaag gctttaccga tagaatgttc atcggtgaca
     6361 ccggtcaatg atgttgccag aataatgact gacaaaccaa tcaagccaac ttccgccaga
     6421 tgcaacgcca gcgcagtcac cagccagacg ccaataatcg cctggacaat cagacggatt
     6481 ttatcctgac gggtacgctg gtggcggctt tgatcgtcaa actgttgcaa cacttcgcgg
     6541 actttctccg gcagcgtttc accgtagcca aaccaacgca gcttctctac cagcaggcag
     6601 gttaacaggc cacaaatcag aaccggaacg gtcaccggcg acatgcgcag gaagaaatcg
     6661 ccaaaatgcc agccagccgc tttagcgatg atcaggttct gtggttcgcc caccatggtc
     6721 attacgccgc ctaatgcggt gccgacaccg gcatgcatca tcaggctacg cagaaagcca
     6781 cggaactgtt ccagaaccac gttgtaatgc ttgtcgatat gactatcgtc ttgcaggtcg
     6841 gtgtcttcgg tacgggaaga ggctacgcga tgataaatac cataaaaacc gactgcaacg
     6901 ctgatcacca ccgccacgac ggttaaggca tcgaggaacg cggagaggaa cgctgccgcc
     6961 acgcaaaaag agagcgacag cagcattttg gagcgaatgc ttaacagcaa acgggtaaat
     7021 atgaacagca acagctgttt cataaaatag atacccgcca ccataaacat caataacagc
     7081 aagacttcaa gatttgccgc cacctcttca cggacgtgtt ccgcgctggt catgccgatg
     7141 aataccgctt cgatagccaa cagaccaccg gggagtagcg ggtagcattt cagggccatc
     7201 gccagagtga aaataaattc cgctaccagc aaccagcccg cgacgaaagg gctgatgagg
     7261 aaaattaacg ggtttacgat taagaaaatg atgagggcga gtttgtacca gtcgggggac
     7321 tggcccaaaa aattgcgcca tagcgcgcgg ccccaggaga tctccatgat ggtttccctt
     7381 accttacaaa taatcaatga tgtttttatg tttaaacgca aagcttaacg gtcaggcagg
     7441 agtgaggcaa gtcttgatag tcaaggggaa agagatgcgg aaaatgaagc cttgatccct
     7501 ttttcttctt tttgtctgct atcagcgtag ttagccctct ggtatgatga gtccaacttt
     7561 gttttgctgt gttatggaaa tctcactatg gtcattaagg cgcaaagccc ggcgggtttc
     7621 gcggaagagt acattattga aagtatctgg aataaccgct tccctcccgg gactattttg
     7681 cccgcagaac gtgaactttc agaattaatt ggcgtaacgc gtactacgtt acgtgaagtg
     7741 ttacagcgtc tggcacgaga tggctggttg accattcaac atggcaagcc gacgaaggtg
     7801 aataatttct gggaaacttc cggtttaaat atccttgaaa cactggcgcg actggatcac
     7861 gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc gtaccaatat ttccactatt
     7921 tttattcgca ccgcgtttcg tcagcatccc gataaagcgc aggaagtgct ggctaccgct
     7981 aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg attacaacat attccgcggc
     8041 ctggcgtttg cttccggcaa cccgatttac ggtctgatcc ttaacgggat gaaagggctg
     8101 tatacgcgca ttggtcgtca ctatttcgcc aatccggaag cgcgcagtct ggcgctgggc
     8161 ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc acgatcaggt gtacgaaaca
     8221 gtgcgtcgct atgggcatga gagtggcgag atttggcacc ggatgcagaa aaatctgccg
     8281 ggtgatttag ccattcaggg gcgataatcc cttccgttta aagagcaacc ccctcaaacg
     8341 aggggttttt tgttgttttt acagatttcc cattcttggc gggcaacgtt ccagcaactc
     8401 gatgctgccg tcttcgtttt gctgttcgag catcacatca aatccccaca ggcgatgcac
     8461 atgcttcagg acttctttgc gcccccgatc cagcggtgcg cgattatgtg gaatataacg
     8521 cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag atctgaatat tcggctccag
     8581 attacttaag ttatattgcg acgataaccg gttacggatc tcccgataac cttcttcatt
     8641 atgaatagcg gaaatctcca gataattatg ccgatcatca tccagcacgg tgaagaagcg
     8701 gaaatcacgc atcactttcg gtgacagaaa ctggctgata aaactctcat ctttgaaatc
     8761 acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg gcgatatccg ggaaccagta
     8821 tttgtcttct tccgttggcg actgacaaat ccgtttaata tcctggaaca tggcgaaccc
     8881 gagggcatac gggttgatgc cgctgtacca cgggctgtta taggggggct ggaagaccac
     8941 attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc gttactttcc cttcatcata
     9001 cagatggtta aggatggtat agtgccagaa ggtcgcacag ccttcgttca tcacctgagt
     9061 ctgtttttgc ggataaaaat actggctcac cttacgcaca atacgcagga tttcacgctg
     9121 ccatgattcc agcagtggcg catttttctc cataaaatag agcaggtttt cttgtggttc
     9181 ggacggatag cggcgcgctt cagcaaccgt tttctcttcc tcgcgcttcg gcagggtacg
     9241 ccagagcata ttgacctgac tttgtagata ctcttcgcga cttttctgcc gggctttctc
     9301 ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc acgccgtagt tcatcagcgc
     9361 gtggcacgag tccagaagcc gttctacttc atcaacgcca taacgttctt cgcactcggt
     9421 aatatattta cgggcaaaaa tcagataatc gacaatcgaa ctggcgtcgg tccaactgcg
     9481 gaataagcaa ttgtttttaa agaaagagtt atgtccatag caggcgtgtg ccatcaccag
     9541 cgcctgcata gtaatggtgt tctcttccat caagtaagcg atacacgggt tagagttaat
     9601 gacgatttca taggccagcc cttgctgacc gtgcttatac agccgttcag tctcgataaa
     9661 ctttttaccg aatgaccagt gcggatagtt aattggcatg ccgacgctgg aataggcatc
     9721 catcatctgt tctgaggtta tcacttcaat ctggtgcggg taggtatcca gccggtagag
     9781 tttcgccacc cggtctatct ctgccagata aacatccagc aggtcgaacg tccagtcggg
     9841 tccatcgctc aaacgtgtgg tgtccttatt catagaatcg atcgtcgcca tacacgcacc
     9901 tcattgttgt cggcgctctc tgtgtggagc acctcatttc aagcatagaa cacctgttaa
     9961 aaaccgcgtc gccggagaat ttttttcttt gcgatttctt attatcagag tgccactaat
    10021 ccgcttctga acggaatttt atgctggata aaaagggcgt tcagcaggag atactaaaga
    10081 cgccatattg ccgcagagtc agggagatgt gagtcagctc accataaaaa agccgtatgt
    10141 tgaataatat tttcaactga gttatcaaga tgtaattaga ttattattct tttactgtat
    10201 ctaccgttat cggagtggct atgcgagttg tcatactggg aagtggtgtg gtaggcgttg
    10261 ccagcgcctg gtacttaaat caggcaggac atgaggtcac cgtcattgat cgggagccgg
    10321 gggccgccct ggaaaccagt gccgcgaatg ccgggcaaat ctcccccgga tatgctgcac
    10381 cgtgggcagc accaggtgtg cctttaaaag cgattaaatg gatgttccag cgccatgcgc
    10441 cgctggcggt tcgtctcgac ggtacgcagt ttcagttgaa atggatgtgg caaatgttac
    10501 gtaactgcga caccagccac tacatggaaa acaaagggcg gatggtgcgt ctggcggaat
    10561 acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa tattcagtat gaagggcgtc
    10621 agggtgggac gctgcaactg ttccgtaccg aacaacagta tgaaaatgcg acccgcgata
    10681 tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct ggaatccagc cgcctggcgg
    10741 aagtggatcc cgcgctggca gaagtagcgc acaaactgac gggcggcctg cagttaccca
    10801 atgatgaaac cggagactgc cagctgttta cccagaatct ggcgcggatg gcagagcagg
    10861 cgggggttaa attccgcttt aatacgcccg ttgaccaact gctttgcgac ggcgagcaaa
    10921 tctacggcgt gaagtttggc gatgaagtga ttaaggccga tgcgtatgtg atggcgtttg
    10981 gttcttactc gacggcgatg ctcaaaggca ttgttgatat tccggtgtat ccgctgaaag
    11041 gctactcgct gactattcca attgcgcagg aagatggtgc gccagtatcc accattcttg
    11101 atgaaaccta caaaatcgcc attacccgtt tcgataaccg cattcgtgtt ggcggaatgg
    11161 cggaaattgt tggttttaac accgagctgt tgcaaccgcg tcgtgaaacg ctggaaatgg
    11221 tggttcgcga tctctatcca cgcggcggtc atgtcgagca ggcgactttc tggacgggtt
    11281 tgcgcccgat gacgccagac ggtacgccgg ttgtcgggag tacacgcttt aaaaatctgt
    11341 ggctgaatac cggtcacggc acgctcggct ggacgatggc ttgcggttcc ggtcagttgt
    11401 taagcgatct gctctctggt cgcacgccag cgatcccata tgaggatcta agcgtagcgc
    11461 gctacagccg tggatttacg ccatcacgtc cgggccattt acatggcgca cacagctaag
    11521 gaaacgagat gacccgtccg atacaggcca gcctcgatct gcaggcatta aaacagaatc
    11581 tgtccattgt ccgccaggcc gcgccgcacg cgcgcgtctg gtcggtggta aaagcgaacg
    11641 cttacgggca tggtattgag cgtatctgga gcgcactcgg ggccaccgat ggctttgcat
    11701 tacttaacct ggaagaggca ataacgttac gtgagcgcgg ctggaagggg ccgatcctga
    11761 tgctggaagg atttttccat gctcaggatc tggagattta tgaccagcac cgcctgacca
    11821 cctgcgtaca cagcaactgg cagctcaaag cactgcaaaa tgcgcggcta aaagcaccgt
    11881 tggatattta tcttaaagtg aacagtggga tgaatcggtt aggcttccag tccgatcgcg
    11941 tgcttaccgt ctggcagcag ttgcgggcaa tggcgaatgt tggcgaaatg accctgatgt
    12001 cgcattttgc tgaagcggaa catcctgatg gaatttccgg cgcgatggcg cgtattgagc
    12061 aggcggcgga ggggctggag tgtcggcgtt cgttgtccaa ttcggcggcg actctgtggc
    12121 acccggaagc gcattttgac tgggttcggc ctggcattat tttgtatggc gcttcgccgt
    12181 ccggtcagtg gcgtgatatc gccaataccg gattacgtcc ggtgatgacg ctaagcagtg
    12241 agattattgg tgtccagacg ctaaaagcgg gcgagcgtgt gggctacggc ggtcgctata
    12301 ctgcgcgcga tgaacagcga atcggcattg tcgccgcagg atacgccgac ggttatccgc
    12361 gccatgcgcc taccggtact cctgttttag tggacggcgt gcgcaccatg acggtgggga
    12421 ccgtctcgat ggatatgctg gcggttgatt taacgccttg cccgcaggcg gggattggta
    12481 cgccggttga gctgtggggc aaggagatca aaattgatga tgtcgccgcc gctgccggaa
    12541 cggtgggcta tgagttgatg tgcgcgctgg cgttacgcgt cccggttgtg acagtgtaac
    12601 ttgttgtaag ccggatcgga ggcagcgtct tctggttgcg aaaaaatcat ccatccggct
    12661 ggtcagcaac tgtagttgtt aatgtgacag agccattgcc catgatagtg tccattaaaa
    12721 gaatggacac tatttccccg gaacctgaac tcgccgcaca ggcgttctac ataaaacgct
    12781 tacgcttcat tgttgactcg gagtcgactt cagataaatc acgcttcacc cttgatgcag
    12841 attcgggttc atgttcagat tgttgattaa caggctgcga gtcctgttgt ctttccagac
    12901 tcaacaggcc caactgttga tccaggagct ccaggtcctc ctgtaccttc acgcgctcca
    12961 ggagttccgg gtcatccagg aactccagat ccataaggtc ctccagctcc tcgagtcctt
    13021 ccaggtcaac ctagccttcc ttgtgcaaaa agtccaaaag gtccttcagg ccctgctgtt
    13081 ttttcaggcc ctcgacggct ttgagcagcg tcggtaaatt gtctttgtcc cacgggttac
    13141 cattacagtc aaacaccttc aggctctggg gctgcggcac ctgtaccgtt ttaaacttgt
    13201 tttctccaca gtagtaaatt attagtttgg gcggcagccg tggtgaagag atcaaatcgt
    13261 tactagaaca gtcaaatact ttcaggcgag tcaagttctc cagcatctca tcaggtattg
    13321 agttaatatt gttgccggca caggatagtt tatacaggta ctggggcatc gacggcggta
    13381 aaaattgcaa gaagttttca gaacagttaa tcactctcag gttagtcgac agctcaggca
    13441 gccagataat actgttttca tagcacttca gtaccatcag ttttgagggc tgcaccttcg
    13501 gtaacaagat caagacgtta tctgtacagt caagcaccag cagccacttt ggcatcacct
    13561 tcggtaacgc ggtcaacacg tttttactgc agtaaaattc aatcagatgt ggaggcaaat
    13621 tctcagggag tgaagtcaat cccaggttgc taagattgag catactccgt cttgtaatga
    13681 gacattcctt cattctgcgc aatgcttctg cccggttttc aatccggctt gctggcaggt
    13741 tttcttccag gatattttcc ttgatttctt ccagactttc tttcaggtct tcttggggaa
    13801 tttctgactg tttgttttgc tggttttctt ccaggatttc gtacaggttt ttttgcaggt
    13861 tttcttgcaa accatcctgc tgtatagccc attcgcacca tttggcgacc atcatgaact
    13921 cgttatgttg ctgattcaga cgagtcccca acgtgatgat ggtattgttg tccacctgta
    13981 tgttcacttt gcccgcgata ctgaggtcaa tgatctgcca gggggcatca ccccagctga
    14041 atttttgcgg gctgagatac ttcatgttct gcaaaatttg attcttgaga agagccgccc
    14101 tggcgggcgt cgtacgcctg ggttaacgga agatggggga atggcggggg cgaatgggca
    14161 gatgtcttta aagctagcca taatgctacg tttccttgaa ataaatatcc tctggcagcg
    14221 ctggagacgt gaatgtggtc accgcaaagg agccacagga cgtcatttgc gctgatatca
    14281 ctactcatcg ttgacttttt ccctggtcag aaatgccgtg tcgggtctgg tacatgctgt
    14341 attacggcct aggtagctct gacgtattaa agaaaatacg agagtgaacg acccggaaat
    14401 aatcgttatt aagcaaagga ataactataa gaaatcgctc atgcatccgg tgctacaggc
    14461 ggatgaaacg cctattgtgt gggctatgtc agtgcggcag gttgcgtggg ggaatatgtt
    14521 caggcgatgt caggcaggag cccctgatgg ttgaccggta atgcggggct ggtttttatt
    14581 acctgttaca gtcggaaaaa gcccttacgc tgactttctt gagccggtgt tttgagttca
    14641 ttagccagag cctgcaatgg tttgtcatcc ataatttaac cttcttctga tgttgggatg
    14701 agcatatcaa aatcaggaaa tgacaagttt attacaggct caggctgcgt tcagtaaagc
    14761 atcgcatccg gcgatagatg ctgcattaaa tttaagattc agcttcctct tcagccaccc
    14821 gaacgccaat cttcaacact tcattatctt ctttctcggc caccgtccag atcatcccgg
    14881 caaactctac ctggtcaccg acaaccggtg ccgcgcctag caactgctgg acaatttcac
    14941 ccagcgtttg ctgtttatca cgatattctc gcccgtcttc caggccatat atcagcgcaa
    15001 catcagcata tttggcgctg gcttcgagaa tgaagtcacc aaagaagcgt tgatccagcg
    15061 cgaccggtgg cgactggctg aacagtttac cgagcgccgg gagatcgcgt tcccgaccaa
    15121 ttacacacaa tacatcgcct tcgcgcagtc gggtgctgcc ggtaggatga agcaactggt
    15181 tatcacgaaa cagggctgca atacgcgtct cttttggcat atgcaaatcg cgcagtgccg
    15241 cacctacaca ccatttatca gcactcaatt gataaacaaa ctgctcccac ggattttccg
    15301 gatgaatatc caggccaacg cgtgacaccg gacgtccgac gggcggaacc accactttgg
    15361 cttttttcgc cgcccacgag agtgatgttc cctgcaagag cagtgaaacc agaaccacaa
    15421 agaaggcgac attaaagaac agtcgcgcat tctccagccc cgccatcatc gggaacacgg
    15481 ctaggatgat tggcaccgcg ccgcgtaatc ccacccagct gataaacacg cgctcacgca
    15541 gattgaaacc gcggaaaggg agcaatccag caaataccga aaggggacgg gcgaagaata
    15601 tcatccatgc cgacaaaatg agcgccggaa tggcaatagg cagcagatcg cttgggttaa
    15661 ccaatagccc cagcaccagg aacatggcga tttgcgccag ccaggcgagg ccgtcgaaat
    15721 tttgcaggat gccgtagcgg ttgcgaatcg gccgattacc cagcagaaaa ccgcacagat
    15781 aaacagccag aataccgctg ccttccagcg ccgtggttaa agcaaaaatt aaaataccgc
    15841 cgcttaatgc cagcaatgga tataatccgg caggcagggc gatgcgatta atcatttgca
    15901 gcagtaaata accaccgcca agaccgatga caatcccgag gccaaattgc tgcagaatat
    15961 cgaccacgaa catccaactg acactgcttt catgctgctg gatcattgca atcagggtaa
    16021 tcgtcagaaa gaccgccatt ggatcattac tgccggattc aatttccagc gtcgagccaa
    16081 cacgttcgtt aagcccctta ccacccagca gagaaaagac cgctgcagca tcggtggagc
    16141 cgacgatagc gccgattaat aagccttcaa tcaaatcaag attaaacagc cacgccgcca
    16201 tcatgccggt taaaccagag gtgataagta cgcccagcgt cgccagcgac agcgccggtc
    16261 ctaacgccac acgaaaggaa ctggcctgag tgcgcatccc gccgtcgagc aaaataatcg
    16321 ccagcgccag gttacttacc atgtaggcga aggggtaatt atcaaacggg ataccgccga
    16381 cgccatcgac tcccgccagc atgccgatcg ccagaaaaat aaccagaata ggaatgccaa
    16441 gacgggaaga aaatgaacta agtaatatac tgctggtgac aaggatggat cctaagatga
    16501 aaaggctaat tattgttgtg gcatccaacg gtcggttact cctgattacg ctgtctctta
    16561 tataaaccct accatattag cggcagagac agcgttttac ttagccctga agcgtgattt
    16621 ttttacattt taagaacagg atgaccgcta atcgtcaact gagcgccttc ccgggtgtta
    16681 ttcagaatgg catgtgcgcc caacggcagc gtaaccgtgc gttgttcatg accaaaatcg
    16741 aggccggtaa tgagtggaat cgacaggcgg gaacgcaaaa acgcgtacac tgactccagg
    16801 ttgtaacctg cgtcataatc attgggcgtg ctgccgctaa agctaccgag aataatcgcc
    16861 ttctgacgtg gcaaaattcc cgcatgatag agctgcaaca gcatacgttc gacgcggaaa
    16921 gggtgcacgt taatatcttc cagcaccaga ataccgttct caatttttgg catccacggt
    16981 gtaccaatca gtgaaatcag catcgcaaga ttgcctcccc acaacgtgcc ttcggtctga
    17041 catgtcgggc cttccccttg ccattcaatg gtgaaggttt cattgcgtaa cgccagccag
    17101 aaatggtgct cggtaaaggc gttcagttca tccgcaccaa agtttgctac cagcatgggg
    17161 ccgctaaagg tgatgacatt gccttgcgcc agaagaccgc actgaatggc ggtaaaatcg
    17221 ctatgtccgc aaatgagtaa cgggtcatgt tgttggcgag ccaccagtgc ctgccagtca
    17281 atatctgcca gtaaacgact ggcaccgtaa ccgccgcgta cagccagcac gatggtgttg
    17341 ggggtagtca gtctggcaag ggaattgaga tcttccagac gctccgtttc cgtacccgca
    17401 aaacgctcac aacgacgggc aataacctcg acgttattga cttgatgccc cgcgtcggta
    17461 aggcgctgga taccacgcag cgcggcgtgc tgtttaatgc tgtaacccga tggggcaatt
    17521 aagtgaaaca gagacatggc aattccttgc tgacaacaga aacgaaatgt atatcatgcc
    17581 gcttaggtgt gccgttgtca cctcaacggc gattccaggc tataaggata gaagaagtga
    17641 aattgagatg gtttgccttt ttgattgtgt tattagcggg ttgttcatca aagcatgact
    17701 atacgaaccc gccgtggaac gcgaaagttc cggtgcaacg tgcgatgcag tggatgccaa
    17761 taagccagaa agccggtgca gcctggggcg tcgatccaca attgatcacg gcgattatcg
    17821 ctatcgaatc gggtggtaat ccgaacgcgg tgagtaaatc gaatgccatt ggtttgatgc
    17881 agttaaaagc ttcaacctcc ggacgtgatg tttatcgccg tatgggctgg agtggtgagc
    17941 cgacgaccag cgagctgaaa aatccagagc gtaatatttc aatgggggcg gcttacctga
    18001 atattctgga aaccggcccg ctggcaggca ttgaagatcc gaaggtactg caatatgcgc
    18061 tggtggtgtc atacgctaac ggggcaggtg cgctgctacg gactttctcg tcagatcgga
    18121 aaaaggcgat cagcaaaatc aacgatttgg atgctgacga gttcctcgat cacgtagcgc
    18181 gaaatcatcc tgcgccgcag gctccgcgct atatctacaa acttgagcag gcactggacg
    18241 cgatgtaaat cagtcgcgca ctttgtccgc tttttcccgg gcttctcgct cgagagagaa
    18301 aataatccgc tgtaattgcc gctccaccgt cgggctgacg ttaagaaaac ggaagctcag
    18361 acggggagtg gtgatggttt cattcttgcc atcaatcact ttgcgctcgc tggtggagat
    18421 taactgggcg tcaaagtgaa aaacacccca ttgccccatg ttgacttcaa tttgagcgaa
    18481 gcgcatacct tcttgtaatt cggcaggctt tgctgtttcc agtaatgcgc ccatgccgcc
    18541 taacgacaaa tcatacaagc ggaaacgtaa cgtactgtta tccgccagtt tggtctggca
    18601 aaaataaggc ggatggagtg gggcggagat gcggaaatat cggcgtcgtt gtacaaacca
    18661 taaggtggga ggcggtacgg taataaatgc cggaagctgc aagtattcac tctgctgtag
    18721 ttgttcaaca gtaaactcga ctttcgcacc ctgagtttcg gcggtaatgg taatgtgctg
    18781 tgcctttagc acggcgatgt tgtcttcggc ttgactgccg aaatccagca ccagtttatc
    18841 cggggttatt gccagtattt tgctgatcag ctgcccgcca ttccaactga gacgcaaagg
    18901 aattgcggct ttgtgcaaat cgcgtaacac gcccaggacg gctaacggat tttgtttcag
    18961 gaactgctca tggtaatgac tcacgcggaa aaactcctga ctgacaaact gtctttggtt
    19021 tatcggtcat agttaacaaa acttaataca aatgcgtgaa tattttttac atgttgttct
    19081 taaatcagcc gacaaacgcc cagcatcgat cccgcccttg cctatactta gagcgttgac
    19141 gtaagcattt cttgcgaccg attcatcgaa acgagggcct gaacatggga attattgcct
    19201 ggattatttt tggcctgata gccggcatta tcggcaagct aatcatgccg gggcgtgatg
    19261 gtggtggatt tttcctgacc tgtattctcg ggatagtcgg tgcggtggtc ggcggctggc
    19321 tggcgaccat gtttggcatt gacggctcca tcagcggttt taatttgcac agcttcctgg
    19381 tggcggtggt gggcgctatc ctggtgctgg gcgtattccg cctcctgcga agagaataag
    19441 attttcataa ggcggatagc gatacagatg ccgctatccg ctttcacatc agaacgtata
    19501 ctcgacacct gcccagaaat tgcgaccttc ttcaataaac ccttcactgt aggcatattg
    19561 ggtatcaaac aggttattga ccgacgcatt gacgctgaag ccatgaccta aggtgtaatc
    19621 ggctcgaatg tgggtcaccg caaaaccggc ggctttttgt gaaccgtcac tgttgctgta
    19681 gctggaggaa cgcgcctctt ccgacagcgt tacgcttaac ggctcccatg gtttgagtgt
    19741 catccatgcg gtcattgtct gcgttggaag atcggttatc ttgccgatgt ctttgcgttt
    19801 ggcgtcagcg tggatcagag cgtagctcaa tcctacatcc agtatattgc tgattttccc
    19861 cttaataccg gcatccagac cgctgtaatc caccgtgccg ctgttctgat tttgaatggt
    19921 atcggcattg atattgtgcg agaggatggc atcactcacc cggttatagt aaacgctgac
    19981 ctcaaatccc cagtcgcgcg tgaaggcacc attccaggtt aaatccaccc cgcgagcgcg
    20041 ttccggcttg agctgcgggt taacaatcgc tatctggtta tacgcaggtt tggatgtggt
    20101 atagcgttct ttcagcgtcg gaaagcgtgt gcggtcatag tacgaaagcg ccagagtgtc
    20161 ttcattggca aagtggtatt tccccatcac ctgccagtta aaagctgact gattgttgtc
    20221 gtcataatgg gtgatgctgc catctttctc gtgttttttc gcttctacgc tatcgcgcca
    20281 gtcatagctg attcccgcca cgacatcgac attatcagca gcagcccatt gatattcact
    20341 ggcgagcgac caggtacgat cttcatagcg atcgtaagcg gcgtgcggcg cacctttttc
    20401 ccggtgtacg tcatctttcc agttaacggc aaacgacagc agatcgtttt cacgcacatc
    20461 ggctgccagt tgtaatccgg caccgtcgct gtaatcggaa taatggctgt agctgccttt
    20521 tttatttttc aaatcagcca gcgagttgta catcatcagc gtattttcaa aggtgtcgcg
    20581 atacagccga cttttcaggg taaaacggtc gtttagttgg gtcgctccct gataataaaa
    20641 actttctttg tcatactctg gccactgcca gtagcgtgat ttttgaccac aatttccgct
    20701 gtatggcggg ttatcttttt caccatcctg cttaatgtaa gtcagcgtgt attcatcgtt
    20761 ttcgcgcggt gtaaaaccga gcttcacaat gccgcgttta tcatcagccg atgaattaat
    20821 catcttgccg tgtttgcctg caatatcatt atttacacca tgcggcaggc cgagaaaatc
    20881 ctgctttagc tggctaccgc tgacttgcaa atacccaaga tcgctgctgg cggcaaatga
    20941 agcatgcata tcgtaggcat tgtcctgact acggctccat ccctggcgat atcccagact
    21001 tgcttcttca ggggggtgat attaatggct ccgcccatct gattaggccc atgaagcagc
    21061 gacgaatacc ctttggaaac ttcgactgcc cccagattgt tggtcagaat ccgcgccaga
    21121 tcgaggttgc cgtcataggg gacataaatg ggcacaccgt cgaaatagac cggcacctgc
    21181 cgactatcaa agccacgaac ttttacctgt tcttcgttgc ggctgcctga cttttgcagc
    21241 accacgccgg ggacgacact taatgcctga gcgacattct gtttatccaa ggccttcatg
    21301 gtgggttgat cgagaacggt ggtcgtcgtc gatgataccg gactggacca tacggtcagc
    21361 gtgtcgctct cctgagccgc tacggcctgc tgggtaaagg caagtgataa tgctgtgcag
    21421 agataacgtt ttttgagtct catagaataa attccgttta acaaagaatt aaatagaggc
    21481 tgtgggttgc agactgactt taatgtcagc tggtgccgcg taataaggcg cggaggtaat
    21541 aaaaaggcga atgccgcagt cgaggtaatt tttgagtgtc gtcagattaa ttccgccggt
    21601 cagagccagc gtgcagtgag gagccagcga cggcgctatt tgcgcaattt cagttgcctg
    21661 ttgcggacta aatttgtcga gctgaagtac gtctggttgt gcgcgtaacg cggcgatagc
    21721 ctctttcggc gtgtcggctt caacaacaat tttcttctcg ggtgcgtggc gacgtaactg
    21781 agcgattgcg cctgaccagc cctgattgtc atgaagaaaa tggcggtggt tggcaaacaa
    21841 taatatggtt tccgcacatc cggcacgatg aattagtcct ccggcagcca gaattgcctg
    21901 cgaagccagt aagcgggtgc ccggaattgc ttttcgggtg caggcgatat taccgtcagg
    21961 ataacgttca cgaagcaatg ccagcatttg agcgagataa tcagaaacac cgcaactcca
    22021 ctccagcaca ttctggactg ccttccatcc ctgatgaagt gctgcggcat taccctgcgc
    22081 acggattagc cgttgacccg cgttcgcttg tgaaccgtcg ctgaccgcgt catcaatagt
    22141 taatcccagc gtagttaaca tcttacacgc gacagaaacg ccgctgacgc agccgccctg
    22201 acggtgaaaa aacgctatat agccaggctg gtgtccaata tttaatgctc gcgtggtcag
    22261 gtcacctccc tggatatctt ccagcagtaa cgcatcgatt tgtgcctgag aaaggaaaat
    22321 catcacagtg cctcctgcgg tacgctatcc caccagatga gcgcccagtc gcggctggca
    22381 ggggcgaggg cacgagcatc ttgttgttga taccagtgat ataaccgctc acgctcatcg
    22441 tcgttaagcg cgccaagact ccagctgacg ttctgctcaa aacgttccca ggtgctgtta
    22501 tcctgttggc aattttgtcc acggataaaa tcaacgtggg cataaattcc catctgatac
    22561 aggacgttga gcgcaaagat atagttgggt aattcgatca cttcccgacc agccgcccgc
    22621 tgaatggctg gagagacgaa cgaggtgctg accagatgag ttgtatagac gcgaagccgt
    22681 gcctgattat tcagtttgct cattgcatgg cgcatatcag ccaccagagt ggagcgggaa
    22741 gccacggcaa tatcgcagcg tggcaggtca ctccagtcct cttcccaggc gcgctggatc
    22801 cagtggacat tgtcagcctt cagtgcggcg gcgcggcggg cggcaacgtt cagcatcccc
    22861 tgactgtaat caacgccata aaccgtagtg agtttgtcag ccagcgccag cgatacggtg
    22921 cctggtccgc agcccatatc gaataaggtc tgagcgcctt gcaggtcaat tttagcgatc
    22981 agttgttgca ggtagctgtc cgttggcgag gcgcaatttt ccgccatttt ctccgcccgt
    23041 tgatcccagt gatcgggcgt tttttctgtg cggtgagcca gtttgagttg ctgcagataa
    23101 aggtctgcaa aatcaatatc atcaatgagc atgattgttc cttagcggct aattgcagag
    23161 aggtgatgat gaatttggtc ggcagaaacc cgatacaggg ccgccagttt gttcgttgtt
    23221 aactgttcgg tcggtaagcc ttgtgttact cgtccatctg gctctacttg gataatgctg
    23281 tcggcaatgg cgttggcatg gagcggatgg tgagtcgaca ttaacattgt catgccgtta
    23341 tttttgagct gcgccagcgt gtccagaagt tggatctgat gaccgaaatc gaggctggag
    23401 gcgggctcat ccagtaacaa caagcgcggt tgttggacga gtgcacgggc gattaaagcc
    23461 agctgacgtt caccgccgct aagcgtattc cagcgtcgtg tcgccagatg caaaatgccc
    23521 agcttttcca gttgctccgt ggctttcagg cgttcttgct taccgggaac ggaaaaagcg
    23581 cctatggtgg gggccaggcc catcaacacc atatccagca cactgaaagc aaaaataccg
    23641 tcgtgcgcct gcggcaccca ggcaatagcc tgtgcgcgct ggcgatggct gagtgtctgc
    23701 acagagaggc tatccagtag gatctcgcct tttaataacg gtataacgcc aagaatggag
    23761 cgcatgagtg tggttttgcc gcaaccattc gcgccaagca gacaacaaat tgttccttgc
    23821 gacagggtga aggacacgtc gcgtagcact ggatggcggg agtaacccag agacgcatga
    23881 cgaacggcaa gcaaagtcac tggcgacctc cgcgtaacaa caaaagcagg aaaaacggtg
    23941 cgccaacaaa agcggtcaga atgccaagtg ggatttctgt tgttccaatg ctgcgcgcca
    24001 gcgtatcggt aagcaggagc agaatagcac cagtacacat tgccataggc aacagctgct
    24061 gatggttatg accggtcaac aagcgggcaa tgtgcggaac gaccagaccg atccaaccaa
    24121 ttatgcccgc tattgctacc gtgctggcgg tgattagtgt ggcgcagaca atcaatccaa
    24181 agcgcagacg cgtcacgttc agtcccagcg aacgggcttc gtcgtcggaa agcgtgagta
    24241 aattcatgcg ccagcggaga aaaaacaggg gcaaggagcc tatgagaatg atcggtgcag
    24301 catagcagag atcgcgtaag gtcacggtgg acagtccccc cagcaaccag aaggtaattg
    24361 acggtaactg ggtgtaagga tcggccagcg ttttaattag cgatatcccc gctccgcaca
    24421 acgttcctaa cgcaatgcct accagcacca gggttaagat cggatcgtgg cgggtcaccc
    24481 gacgcgtaat caaccagacg cccgcgacca ccatcagccc gccgcaaaat gcgtacagct
    24541 ggatgtaaac aatgggtaat ccccacagga ttgccgcgca ggcaccaagt ccggcaccgg
    24601 cggagacgcc taaaatatcc ggtgaaacta agggattacg caacattccc tgataggtgg
    24661 ttccggctcc tgccagcgct gcgcccagta acagcgcagc aagaatacgc ggcacgcgga
    24721 tttgccagaa gacaatttgt tcctgcacag actcattttc ttgacgaaga attagcgcca
    24781 gcagttgctg catatcgaga tgataagccc cacttacggc ggcaaaagtg atactgatag
    24841 ctgccagcag caaaatacat ccgtttacga ttctcattag ctcgccacca atttttgata
    24901 gtcggcatca ctgagtgaac aatgccagaa cagttgggcg taatgctgca tgtcactttt
    24961 aaactggcga ttgatggcgg gatccagcca ggcgtgaagt ctgcgtaatc ccagaagacg
    25021 gttgattccc ggcggggcat cgagccagcc aaagggcaag ccacttaaaa ataagatgcg
    25081 ttgttccgca acggctttca cgccctgcca gagaggatca cgacgaataa aatctgcagt
    25141 aacggcctcc tgaaccagaa taatatccgg ctgccagcgc aggagatttt ccatggaaac
    25201 ctgtgtcagg ccgtggcgat cggctatttg cgcgacgttg tgcaaaccta ataattccgc
    25261 cgcctcggta tgcaacgaac cctgtaagcc cgtttccagc cctcggggac cgcgcgcagc
    25321 ataaaagcgg aggttagcgg cgggtgaggt ggcgaatgcc tgagcttcac caacgaagtg
    25381 gctggcgaga ttggcttgtt ctgcggcgcg gtgctcttcg cctaacgttt tgccaagcgt
    25441 tgttaactgt tctgctgatt gcgccagttt tccgttaagc aataaccagg ggatttgtgt
    25501 ctgttcgcta acctgccgtg cctgggagat caaggtttca tccgtattgc cgcaatcaac
    25561 gaccaaatcg ggatgtaacg ccataagccc ttccagcgat agtgtgctgg cgcgcccggc
    25621 gagtcgtccc agcctgggta actggcgaat gtgctctggc aatggaatta atgcctgacg
    25681 ggcaaaatca aaagaggaaa aaccgaccat tttttctggc gcgacagcca gcaagagcaa
    25741 atcggcgggc gcgcctgcgc tgacgatgcg tgtaatgttt tgggcttcgg gaagagattg
    25801 cgaagcaaaa cgattaactg tttgtgaaaa ggacggtaga gactgcagga gcagggtaga
    25861 tgcaagtgcc tgcgcaaacg tccgtctggt tatgggcatg gagaacatcc tgaaaagtaa
    25921 aatcgatata tactttcata tatatcgata aaaatcatgt tcaatatcaa agtttagcta
    25981 ttgaaaacat gaagatgtga cgacttccgc aagcctgttg accctctcta tcattaaaaa
    26041 tgtatttatc aggtggctaa tattatttaa atcatttaag gctaaatcat ttaaatatga
    26101 atgacattat tgttttttat cgtccacggt tacgcgacgt tgaagttact tgttttataa
    26161 tgagcgattt ttgatatctc gccgagaaaa ttatcagcat tctctcgttt cagtgtcgtg
    26221 aatatactgt taacggaggt gaaaagaatg ctgggggtta tacaaaacct atgttgttgt
    26281 cgtccggatt caccgcttgc tgatagtaag cagcgtaacc agaagttacc gcaacaagac
    26341 tttgaggatt ttaagaacga cgttattaat gtcattaaca acaatgaaga tatttatttt
    26401 cttagagaga gtaatttact gaatgcgttt attcggacgt gcaaaaaaag agatgataac
    26461 agttacggaa atatgatatt tacgctttat tttgataacg atatctatgg gaacagaatt
    26521 atcaaagcat cgctgaacgg cggagagttg cagttattaa gcacagggtg tagcaatgat
    26581 aacattctc
//
LOCUS       DBKELA010000056        26286 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.056, whole genome shotgun sequence.
ACCESSION   DBKELA010000056 DBKELA010000000
VERSION     DBKELA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.056"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..1097)
                     /locus_tag="ACUQR0_002272"
                     /pseudo
     CDS             complement(1..1097)
                     /locus_tag="ACUQR0_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            1105..1534
                     /gene="tnpA"
                     /locus_tag="ACUQR0_002273"
                     /pseudo
     CDS             1105..1534
                     /gene="tnpA"
                     /locus_tag="ACUQR0_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            2108..3190
                     /gene="ompC"
                     /locus_tag="ACUQR0_002274"
     CDS             2108..3190
                     /gene="ompC"
                     /locus_tag="ACUQR0_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HHV9109288.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3679..4477)
                     /locus_tag="ACUQR0_002275"
                     /pseudo
     CDS             complement(3679..4477)
                     /locus_tag="ACUQR0_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4487..5038)
                     /gene="ybcL"
                     /locus_tag="ACUQR0_002276"
     CDS             complement(4487..5038)
                     /gene="ybcL"
                     /locus_tag="ACUQR0_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HHV9109289.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(5207..5539)
                     /gene="emrE"
                     /locus_tag="ACUQR0_002277"
     CDS             complement(5207..5539)
                     /gene="emrE"
                     /locus_tag="ACUQR0_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HHV9109290.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5884..6198)
                     /gene="fliE"
                     /locus_tag="ACUQR0_002278"
     CDS             complement(5884..6198)
                     /gene="fliE"
                     /locus_tag="ACUQR0_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HHV9109291.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            6412..8077
                     /gene="fliF"
                     /locus_tag="ACUQR0_002279"
                     /pseudo
     CDS             6412..8077
                     /gene="fliF"
                     /locus_tag="ACUQR0_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            8070..9065
                     /gene="fliG"
                     /locus_tag="ACUQR0_002280"
     CDS             8070..9065
                     /gene="fliG"
                     /locus_tag="ACUQR0_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HHV9109292.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            9058..9744
                     /gene="fliH"
                     /locus_tag="ACUQR0_002281"
     CDS             9058..9744
                     /gene="fliH"
                     /locus_tag="ACUQR0_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HHV9109293.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9744..11117
                     /gene="fliI"
                     /locus_tag="ACUQR0_002282"
     CDS             9744..11117
                     /gene="fliI"
                     /locus_tag="ACUQR0_002282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HHV9109294.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWKASLQGLE
                     RIFPTVS"
     gene            11133..11480
                     /gene="fliJ"
                     /locus_tag="ACUQR0_002283"
                     /pseudo
     CDS             11133..11480
                     /gene="fliJ"
                     /locus_tag="ACUQR0_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            11477..12604
                     /locus_tag="ACUQR0_002284"
     CDS             11477..12604
                     /locus_tag="ACUQR0_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HHV9109295.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12709..13173
                     /gene="fliL"
                     /locus_tag="ACUQR0_002285"
     CDS             12709..13173
                     /gene="fliL"
                     /locus_tag="ACUQR0_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HHV9109296.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            13178..14182
                     /gene="fliM"
                     /locus_tag="ACUQR0_002286"
     CDS             13178..14182
                     /gene="fliM"
                     /locus_tag="ACUQR0_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HHV9109297.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            14179..14592
                     /gene="fliN"
                     /locus_tag="ACUQR0_002287"
     CDS             14179..14592
                     /gene="fliN"
                     /locus_tag="ACUQR0_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HHV9109298.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14595..14960
                     /gene="fliO"
                     /locus_tag="ACUQR0_002288"
     CDS             14595..14960
                     /gene="fliO"
                     /locus_tag="ACUQR0_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HHV9109299.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14960..15697
                     /gene="fliP"
                     /locus_tag="ACUQR0_002289"
     CDS             14960..15697
                     /gene="fliP"
                     /locus_tag="ACUQR0_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HHV9109300.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15707..15976
                     /gene="fliQ"
                     /locus_tag="ACUQR0_002290"
     CDS             15707..15976
                     /gene="fliQ"
                     /locus_tag="ACUQR0_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HHV9109301.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15985..16770
                     /gene="fliR"
                     /locus_tag="ACUQR0_002291"
     CDS             15985..16770
                     /gene="fliR"
                     /locus_tag="ACUQR0_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HHV9109302.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            17055..17678
                     /gene="rcsA"
                     /locus_tag="ACUQR0_002292"
     CDS             17055..17678
                     /gene="rcsA"
                     /locus_tag="ACUQR0_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HHV9109303.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17722..17910)
                     /gene="dsrB"
                     /locus_tag="ACUQR0_002293"
     CDS             complement(17722..17910)
                     /gene="dsrB"
                     /locus_tag="ACUQR0_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HHV9109304.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            18073..18300
                     /gene="yodD"
                     /locus_tag="ACUQR0_002294"
     CDS             18073..18300
                     /gene="yodD"
                     /locus_tag="ACUQR0_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HHV9109305.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18598..19413
                     /locus_tag="ACUQR0_002295"
                     /pseudo
     CDS             18598..19413
                     /locus_tag="ACUQR0_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(19410..21104)
                     /gene="dgcQ"
                     /locus_tag="ACUQR0_002296"
                     /pseudo
     CDS             complement(19410..21104)
                     /gene="dgcQ"
                     /locus_tag="ACUQR0_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(21275..21457)
                     /locus_tag="ACUQR0_002297"
     CDS             complement(21275..21457)
                     /locus_tag="ACUQR0_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodC family protein"
                     /protein_id="HHV9109306.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21536..22453)
                     /locus_tag="ACUQR0_002298"
     CDS             complement(21536..22453)
                     /locus_tag="ACUQR0_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HHV9109307.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22626..23546
                     /gene="yedA"
                     /locus_tag="ACUQR0_002299"
     CDS             22626..23546
                     /gene="yedA"
                     /locus_tag="ACUQR0_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HHV9109308.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23535..24005)
                     /gene="vsr"
                     /locus_tag="ACUQR0_002300"
     CDS             complement(23535..24005)
                     /gene="vsr"
                     /locus_tag="ACUQR0_002300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HHV9109309.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23986..25404)
                     /gene="dcm"
                     /locus_tag="ACUQR0_002301"
     CDS             complement(23986..25404)
                     /gene="dcm"
                     /locus_tag="ACUQR0_002301"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HHV9109310.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25471..26011)
                     /locus_tag="ACUQR0_002302"
                     /pseudo
     CDS             complement(25471..26011)
                     /locus_tag="ACUQR0_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(26051..>26286)
                     /gene="drpB"
                     /locus_tag="ACUQR0_002303"
     CDS             complement(26051..>26286)
                     /gene="drpB"
                     /locus_tag="ACUQR0_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HHV9109311.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6603 a         6745 c         6467 g         6471 t
ORIGIN      
        1 ccatttacat cggcgttcgc tgtatatcca catacctggc atctgaattt actttgtgac
       61 aggcgatttt ctttcgctgt atgaccacag cacgcgcaac gctggcttgt gtacgctggc
      121 ggaacagcaa gcacctgacc gccacgccag agctgcttgt actcaagctg gcggcgcatt
      181 tcataccagc cctgatccag tatcgaacgg tttaaacctg attttgcccg gacattgcgc
      241 cccggctggc tgaccgtacc cgctgctgac tttgacatgt tgctgacctt caaatcctca
      301 atgactatca ttacgtggtt tttgctgacg gtcgttgtga ctttgtgaag gtagtccctg
      361 cggatatttg cgatacagga atgcagtcgc tgtattttgc gtttctgctt ctgccagttg
      421 ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc tcgccagctt cttctggttt
      481 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgaggc aagtttagcc
      541 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagttga tacttcactt
      601 tctgcctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      661 acacccgtga cctgacggct gttccggtag cgcatccagc caagttttgg cagaaaaata
      721 cggctgtttt cctgagcgag cttaacgccc tgcggatagc ggaatgcatc attctgtcac
      781 cgctttttga atcaggggaa cgccgagcgc ttctggaaaa agtttttgca ggcctgctca
      841 gggtctttca gtgactgttg caatggctgt gacggagcat ctttaatcca ttgcgtttca
      901 gtggcatttt tccactcaac caatcaggaa gccattttcg cgtaagggat gtctttgtta
      961 ccggccttat gattctcatt ctgacgtgcc agagtacgac tgaaaacgaa acgacatgcg
     1021 cctgcgaagc gcctcatttc acgcgcctgt tgaccaccgg gtcttaaccg gaatttgaat
     1081 acctgtagtc gtttcacagg gcaaattata ctttggtctg tgaaaaaaac tgatatccga
     1141 cgcggaaggc actgtgtttt cctgatgcat gctcaccggg tctttgttac caagtaccga
     1201 cgccagattt ttgaccatga cgcgacagaa aaactacgca cttacttttc aaatgtatgt
     1261 gcttattttg aagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatctgtta
     1321 attaactatc cccccaaact ggcgatatcc agtctggtaa acaaacagcc tcaaaggcgt
     1381 atcgggtagg ttactgcgac gggatcgacc agatattgct gccaggtatt accgcaaaga
     1441 tgttttgtgg cgtcccggct attttgccag tagctgcgga ggtgcgccaa tatccgtcat
     1501 ccgccaatac attgaacaac agcaaacacc tggtcagatg aaaaaacgag ccttatatcc
     1561 ccgccctgaa ggacgggctt ttacggtgca ctggataagc ctggagatta tctacacctg
     1621 catggacatc attgtactat gccccgtaca tgccccatca catcctcaac atcaaagtat
     1681 tagagccttg caaaccatta tgcgtctcag tttgtctcat ctatcttacg caaaaaaaat
     1741 agaactaata tcaagcggcg ctatattgcg tattaaataa aaccacctca attacattaa
     1801 taaatctaaa taaatatcat gcaaataatt tccatggtta tttttaggct atttaaatta
     1861 aaaaaataaa ttatgagtca atcataaata acaaccttta atataatttg actaccaaat
     1921 ttatattcgt tcacaaaata gtcatgcatt gtgtgcaata gaaacagttc agataaaaat
     1981 agggatctac ttcacaattt aaatgagaaa ctaaaactta catcttgaaa taatctcatt
     2041 gattggatga atatttgtag tgcagtgaca tcatttttaa ataatagttc aaataaaagg
     2101 gctcacgatg aaaaaattaa cagtggcaat ttctgctgta gctgcatcag tactgatggc
     2161 gatgtctgct caggcagcag agatttacaa taaagacagt aacaagctgg atctgtacgg
     2221 aaaagttaat gctaagcact acttctcttc taacgatgca gatgatggcg acacaactta
     2281 tgttcgtctg ggcttcaaag gcgaaactca aatcaacgat caactgactg gtttcggtca
     2341 gtgggaatat gaattcaaag gcaaccgtgc tgaatctcaa ggttcttcca aagataaaac
     2401 ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc tccatcgatt atggccgtaa
     2461 ctacggtgta gcatacgata tcggtgcatg gaccgacgtt ctgccagaat tcggtggcga
     2521 tacctggacc caaacagatg tgttcatgac tggtcgcacc acaggtgttg caacctatcg
     2581 taacaacgac ttctttggtc tggttgatgg tctgaacttt gctgctcagt atcagggtaa
     2641 aaatgaccgc actgacgtaa ctgaagctaa cggtgatggt ttcggtttct ccactactta
     2701 tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa tctgaccgca ctaatgatca
     2761 ggttatctac ggtaacaaca gcctgaatgc atctggtcaa aatgctgaag tatgggcagc
     2821 tggtctgaaa tatgatgcga acaacatcta tctggctacc acctattctg aaacccagaa
     2881 catgactgtt tttggtaata accatattgc caacaaagca caaaacttcg aagtagttgc
     2941 acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct tacctgcaat ctaaaggaaa
     3001 agacttgggt gcgtggggtg atcaggacct gattgaatat attgatgtag gtgcaaccta
     3061 ttacttcaac aaaaatatgt ccacttttgt tgattacaaa atcaacctga ttgataagag
     3121 cgatttcacg aaagcatctg gcgttgctac cgatgatatc gttgctgtag gattggttta
     3181 ccagttctaa tctgattact caaagatatg ttgtgggagg ctttgcctcc ccaacatata
     3241 agtggcgctc tcaagccact ttccttagaa gcactatctt gcttctgact atataaatta
     3301 ccctttattt tctgggacgt ttcaacgccc catttttaat aatactcagt gaacaattaa
     3361 cgtatcaatt agatttaata acaacgatat ccgtacctaa cctgatatct aatgtcataa
     3421 gcatcccttt aattatcccc tcaacctctt gtaaatttcc cactcttgag ctccacatag
     3481 cccaaacgcc ggatgcaaaa aaaatcacac gtgcatctct gaaattgtga tggaggaagt
     3541 gagtatcaat tatatgtgct tataaacgtt cccgattggc acagaagtca ccggattgtt
     3601 caggctccaa taggttgagt atgtcgtatt tattttccaa aataaaaatc gatgctgttg
     3661 ctccttaata aaatactttt actctctgga cgcttttctt agctctcttg gtgttatatt
     3721 aaaacgatta tggaaccttt cagtaaaacg agaaggacac ttgtaaccat tttccctggc
     3781 aatctcgctg atgggtttta ctgtcgtttg tatagcagga caacgcatta tttaacctca
     3841 cgtcgtccaa tatactttga aaacttattc cctcgcttgc cagacggcga tgcaatgtgg
     3901 aaacagaaat gtagagatat ctcgctacct tgtttgctgt ccattttgtt ctaggttcgg
     3961 atagcattag gttataacaa cgacttatta atgattgttt actatctgat aaaagtaaac
     4021 aattaacatg atgtgctcct aatgaaagta gaacgcccat tgctaagtgt tcctgaattt
     4081 gcgttgagaa gcctcgggaa atagatgttt ttagttgctc ccaacaatat attaactcgg
     4141 ggttctgagg taagcagaaa cctgtcgtgt tacgaatttg atcagttatc gcataaaatt
     4201 tttgaaactt ttcaattaga tcaatgggat aataaagcat ttctgcaaga taaagacctg
     4261 cttctggata attcacaata taaaattcat atccacaggg aaataatatt atctgattat
     4321 tatcaacagt tagactatgc gtctcccaat tgataacttt ttttccctga cggatacgac
     4381 ataaagctgg cataagaggc ttaaccctgt gaatctcatg atgtttatgc atccgtattt
     4441 cttcaatctt taggttagac ttatctcttg ccagcatact cttaccctat tttatctcat
     4501 aaactggtgt tatctcagcg gttgcaattt tattggcatt aagcatataa ccaactaacg
     4561 ctccgctgga attagaatcc acaggaatct tatcagtttt tagagcccat actttaaact
     4621 ggtaatgatg gggtttatct cctttgggag gacatgctcc accaaatcct gcataaccaa
     4681 aatcatttcg gccttgaaca gcaccagtcg gaagttttgt accatcacgt cttcctgcat
     4741 cagtgggcaa atatgttaca gttgctggaa tattagcaac agtccagtgc caccatccac
     4801 tgcctgtagg agcatctgga tcatacacag ttacggcaaa gcttttggtg ccttcaggag
     4861 caccagacca ggttaatgag ggcgatgtat taccaccttc acacccaaat ccagaaaata
     4921 catgagacgt tgtaagttgc tctcctgttt ttatttcatt actagtgacc tgaaatgctg
     4981 cggcctgcgc agaaaatgtt atgaatgcca atacacttga aacgataagt tttttcataa
     5041 aaacctcatt gttgtgacct atcgttattt tatttgatgt gccttgatct cattatgaat
     5101 aaaggcgcaa tgatcgcgca aatgcaatca taattataaa ggtgccccga acattagcca
     5161 taatatgcac aaatcagatt cctgttatct caaggagaat tattttttaa tgcggtgcac
     5221 ttcgtgacaa caagttaatg actaacacac cggcacaaat caacatcatg cctatgatag
     5281 ctggcaggtc cagtcgttga ccaaaaaatc cccaggaaag caaactaatc agaacgatac
     5341 cgactcctga ccagatagca taagcaatcc ctgtaggtat ataagccagc gtctgagcta
     5401 ataaccagaa tgatgcacaa tagcaaataa ttgtgccaac agagggccat aaccgtgtaa
     5461 acccctctga aaacttcatt aaggttgtac caatgacctc tgcaagtatt gcgccaccaa
     5521 gataaatata agggttcata gcatattctt tcctgttcat actggagaga attgtactac
     5581 agtttgaaca caactcaccg ttttcatcct ggtgtatgtg tatccaaggg gctctggcgt
     5641 ggcaagagat aaaggcagga acaataatga tcagaaggaa ccccaggcta acgaatgagt
     5701 tcactcttcc tgggagcaaa gcgaagacaa aacatgttat cgcgtttacg gatcgtggat
     5761 ggcagaaaac aaccaggatc aggtatccat tttaaaccag aaattgagtg agtttgcccc
     5821 atccatgccc cacgctgtgg gatcggatat aattaaacaa gcatagtcaa caggttacac
     5881 aacctacacc tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg
     5941 aatccccatt tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttagcgc
     6001 cacgccgggt tcaccgaggg tgaatttttc tgcctgcgtg cgggcgactg tttgtgtatc
     6061 gcttatgcga tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag
     6121 tgattcctgc gcacacgcac tcatcgccgt agcctgtaac tggctgataa ccccttcaat
     6181 cccctgtatc gctgacattt tcgtctcccg gataatttct ggtaacaaag cctaccagta
     6241 agtcaataag acaaaggcgc taaatagcaa caaaaaaact ggtttattgg cggatagaaa
     6301 aaacgaaagc acaaataatg ggagcgtcaa tttttcgagt ttgctgaccc gagagtgagt
     6361 cttgttccac tttgccaata acgccgtcca taatcagcca cgaggtgcgc gatgaatgcg
     6421 actgcagccc agacaaaatc tcttgagtgg cttaatcgcc tgcgtgcgaa tccgaaaatt
     6481 ccattgattg ttaccggttc cgcggcagtg gcggtcatgg tcgcactgat cctgtgggcg
     6541 aaagcccccg actaccgcac attattcagc aatctttccg atcaggatgg tggcgcaatt
     6601 gtcagccaac tgacgcaaat gaatattcct taccgcttca gcgaagccag cggcgctatt
     6661 gaggttccgg cagataaagt tcacgaactg cgcctgcgcc tggcgcaaca aggtttgccc
     6721 aaaggcggcg cggtcggttt cgaactgctc gatcaggaaa agtttggtat cagccagttc
     6781 agcgaacagg tgaattatca gcgggcgctg gaaggcgagc tttctcgtac catcgaaact
     6841 atcggcccgg taaaaggggc gcgcgtacat ctggcaatgc cgaaaccgtc tttattcgtc
     6901 cgtgaacaaa aatccccttc tgcatcggtg acggtaaatc tgttacccgg ccgcgcactc
     6961 gatgaagggc aaattagcgc tattgtacat ctggtttcca gcgcggttgc tggtctgccg
     7021 cctggaaacg tcacgctggt ggatcagggc gggcatctgt taacccagtc caataccagc
     7081 gggcgcgatc ttaatgacgc tcagttgaaa tatgccagcg atgtcgaagg ccgtattcag
     7141 cagcgtattg aagcgatcct gtcacctatt gttggtaacg gtaatattca cgcctaggtc
     7201 acggcgcaac tggacttcgc cagtaaagaa caaacggaag aacagtatcg ccctaacggt
     7261 gatgaatctc atgcggcgct tcgttcacgc cagcttaatg agagcgagca aagcggttcc
     7321 ggttatccgg gcggcgtacc gggggcgttg tcgaatcaac cggcacctgc gaataacgcg
     7381 ccaatcagca cgcctccgac aaatcaaaat aaccgccagc agcaggcgag caccaccagc
     7441 aatagtgggc cgcgtagcac acagcggaat gaaaccagta actacgaagt cgatcgcacc
     7501 attcgtcata ccaaaatgaa cgtgggcgat gtgcaacgtc tgtcagtcgc ggtagtggtg
     7561 aattacaaaa ccttgccaga cggtaaacca ttgcctctca gcaacgaaca gatgaagcaa
     7621 attgaagctc tgacccgcga ggcgatgggc ttttctgaaa aacgcggtga ttcgctcaat
     7681 gttgttaact cgccgttcaa tagcagtgac gaaagcggcg gagcactgcc gttttggcaa
     7741 cagcaagtgt ttatcgatca gttgctggct gccggtcgct ggttgctggt tgctggtact
     7801 gctggtggcg tggttgctgt ggcggaaagc ggtacgcccg cagctaacac gtcgcgccga
     7861 ggcggtgaag actgtgcagc aacaggcgca ggcccacgag gaagtggaag atgcggtgga
     7921 agtccgtctg agcaaagacg aacaactcca acaacggcgc gctaaccaac gactgggggc
     7981 agaagtcatg agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct
     8041 ggtcattcgc cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg
     8101 tcatcctgct gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc
     8161 agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc
     8221 agctaaccga tgtgctggcg gagtttgagc aagaagctga acagtttgct gcactgaata
     8281 tcaacgccaa cgattatctg cgttcggtat tggtcaaagc tctgggtgaa gaacgtgccg
     8341 ccagcctgct ggaagatatt ctcgaaaccc gcgataccgc cagcggtatt gaaacgctca
     8401 actttatgga gccgcagagc gccgccgatc tgattcgcga tgagcatccg caaattatcg
     8461 ccaccattct ggtgcatctg aagcgcgccc aggccgccga tattctggcg ttgttcgatg
     8521 aacgtctgcg ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg
     8581 cgctggccga actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca
     8641 gcaaaatggg cggcgtgaga acggcagccg aaattatcaa cctgatgaaa acgcagcagg
     8701 aagaagccgt tattaccgcc gtgcgtgaat tcgacggcga gctggcgcag aaaatcatcg
     8761 acgagatgtt cctgttcgag aatctggtgg atgtcgatga tcgcagcatt cagcgtctgt
     8821 tgcaggaagt ggattccgaa tcgctgctga tcgcgctgaa aggggccgag cagccactgc
     8881 gcgagaaatt cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg
     8941 ccaaccgtgg tccggtgcgt ctgtcgcagg tcgaaaacga acagaaagcg attctgctga
     9001 ttgtgcgccg tcttgccgaa actggcgaga tggtgattgg cagcggcgag gatacctatg
     9061 tctgataatc tgccgtggaa aacctggacg ccggacgatc tcgcgccacc accggcagag
     9121 tttgtgccaa tggtcgagtc ggaagaaacc attattgaag aggccgaacc cagccttgag
     9181 cagcaactgg cgcaactgca aatgcaggcc cacgagcagg gttatcaggc ggggattgcc
     9241 gaaggtcgcc agcaaggtca cgaacagggc tatcaggaag gactggccca ggggctggaa
     9301 caaggtctgg cagaggcgaa ggctcaacaa gcgccaattc atgcccggat gcagcaactg
     9361 gtcagcgaat ttcaaactac ccttgatgcg cttgatagtg tgatagcttc gcgcctgatg
     9421 cagatggcgc tggaggcagc acgtcaggtg atcggtcaga caccaacggt ggataactcg
     9481 gcactgatca aacagatcca acagttgttg cagcaagaac cgttattcag cgggaaacca
     9541 cagctgcgcg tacacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacttta
     9601 agtttgcatg gctggcgctt gcggggcgat cccaccctcc accctggcgg atgtaaagtc
     9661 tccgccgatg aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt
     9721 ctggcagcac caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg
     9781 ataactttga agccaaaatg gcgcagttgc ctgcggtacg tcgctacggg cgattaaccc
     9841 gcgctaccgg gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg
     9901 tcattgagcg ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta
     9961 acggtcaacg gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc
    10021 gtgtatatgc caaaaacatt tcggcagaag ggctgcaaag cggcaagcag ttgccgctcg
    10081 gtccggcgtt attaggtcgt gttctggacg gtagcggtaa accgctcgat ggcctgccct
    10141 tccccgatac gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta
    10201 caccgattga acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgtcg
    10261 gacgcgggca gcgtatgggg ctgtttgccg ggtcgggcgt tggtaaaagt gtgctgctgg
    10321 ggatgatggc acgctacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg
    10381 ggcgcgaagt aaaagatttt attgagaata tcctcggtgc cgaaggacgt gcacgctcag
    10441 tggtgattgc cgctccggcg gatgtttctc cgctcctgcg aatgcaaggt gccgcctatg
    10501 ccacgcgcat tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact
    10561 ccctcacccg ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacctg
    10621 ccactaaagg ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg
    10681 gtaatggcat tagcggcggc ggctcgatta ccgcgtttta taccgtgctc accgaaggcg
    10741 atgaccagca ggaccccatt gctgactccg cgcgggcaat cctcgacggt cacattgtgc
    10801 tgtctcgccg actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca
    10861 gtcgcgcaat gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac
    10921 agctgttgtc gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag
    10981 gcagcgatcc gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttgc
    11041 aacaaggcat ttttgaacgc gcggactgga aagcgtctct ccaggggctg gagcgtattt
    11101 tcccgacagt gtcataaccc aggagataac ggcagatggc ggaagaacag ctcaaaatgt
    11161 tgatcgatta tcagaatgaa tatcgcaata acctcaatag cgatatgagt gccgggatga
    11221 ccagcaaccg ctggatcaac tatcagcagt ttatccagac gctggaaaaa gccattactc
    11281 agcatcgcca gcaacttaat cagtggacgc agaaagttga cattgccctg aacagctggc
    11341 gagaaaagaa acaacgtttg caggcctggc agacactgca ggaacggcaa tccacggcgg
    11401 cactgcttgc agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc cagcgcgccg
    11461 ccatgaggaa acctgaatga ttcgcttagc gcccttgatt accgccgacg ttgacaccac
    11521 cacattgcct ggcggcaaag ccagcgatgc tgcacaagat tttctcacgt tgttgagcga
    11581 agcattagca ggcgagacaa caactgacaa agcggccccc cagttgctgg tagcaacaga
    11641 taagcccacg acaaaaggcg agccgctggt cagcgatatt cttgccgatg cgcaacaagc
    11701 ggatttactg atccctgtgg atgaaacact tcctgtcatc aacgacgaac aatccacatc
    11761 aacaccgtta accaccgctc agacgatgac gttggctgcg gtggctgaca aaaatacgac
    11821 aaaagacgaa aaagcggatg atcttaatga agacgtcacc gcaagtctga gcgccctttt
    11881 tgcgatgttg ccgggttttg acaatacgcc caaagtgact gatgcaccgt caaccgtgtt
    11941 accggcagag aaaccaacgc tcttcacaaa actgacttct gcgcaactca ctacagcaca
    12001 gcctgatgac gcccccggca caccagctca gccattaaca ccgctggtag cagaagccca
    12061 gagtaaagcg gaagtcatca gcacgccttc gccggtgacc gctgacgcca gcccgctaat
    12121 cactccacac cagacacagc cactgcccac cgtcgccgcg cctgttttga gtgcaccgct
    12181 gggttctcac gaatggcaac aatcattgag ccagcatatt tcgctgttca cccgccaggg
    12241 gcaacaaagt gcagagttgc gtctgcaccc acaggattta ggtgaagtgc aaatttccct
    12301 caaagtggat gataaccagg cgcagatcca gatgatttca ccgcatcagc acgtacgcgc
    12361 cgccctggaa gcagcgctgc cggtactgcg tacgcagctg gccgaaagtg gcattcagtt
    12421 agggcaaagc aacattagtg gcgaaagctt tagtggtcag cagcaggccg cttcccagca
    12481 acagcaaagc caacgcacag caaaccatga acctctggcg ggggaagacg acgatacgct
    12541 tccggttccc gtctctttac aagggcgtgt aacaggtaac agcggcgttg atattttcgc
    12601 ctaacgtcag aggtagcacc gtaatccgcg tcttttcccc gctttgttgc gctcaagacg
    12661 caggataatt agccgataag cagtagcgac acaggaagac cgcaacacat gactgattac
    12721 gcgataagca agaaaagcaa gcgatcgctt tggatcccga ttctggtatt cattaccctc
    12781 gcggcctgtg ccagcgcagg ttacagctac tggcattcgc atcaggttgc cgctgacgac
    12841 aaagcgcagc aacgcgtcgt gccctccccg gtcttctatg cgctggatac ctttacggtc
    12901 aatttgggcg atgcggatcg cgtgctttat atcggcataa ccctgcgact gaaagatgaa
    12961 gcaacccgct cgcggctgag tgagtatttg ccggaagtcc gtagtcgctt gctgttactg
    13021 ttttcacgtc aggatgctgc cgtactggcg acagaagaag gcaagaaaaa cctgattgcc
    13081 gagattaaaa ccacactttc caccccgctt gttgccgggc aaccgaaaca ggatgtcacc
    13141 gacgtgctgt ataccgcttt tattctgcga taacgacatg ggcgatagta ttctttctca
    13201 agctgaaatt gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac cgacagccag
    13261 tgttagcggc gaaagtgaca ttcgtccgta cgatccgaat acccaacgac gggttgtgcg
    13321 cgaacgtttg caggcgctgg aaatcattaa tgagcgcttt gcccgccatt ttcgtatggg
    13381 gctgttcaac ctgctgcgcc gtagcccgga cattaccgtc ggggccatcc gcattcagcc
    13441 gtaccatgaa tttgcccgca acctgccggt gccgaccaac ctgaacctaa tccacctgaa
    13501 accgctacgc ggcacagggc tggtagtgtt ctcgccgagt ctggtgttta tcgccgtgga
    13561 taacctgttt ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg agtttaccca
    13621 tactgaacag cgcgtcatca accgtatgtt gaagctggcg ctggaaggct atagcgacgc
    13681 ctggaaagcg attaatccgc tggaagttga gtacgtgcgt tcggaaatgc aggtggaatt
    13741 taccaatatc accacctcgc cgaacgacat tgtggttaac acgccgttcc atgtggagat
    13801 tggcaacctg accggcgaat ttaatatctg cctgccattc agcatgatcg agccgctacg
    13861 ggaattgttg gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga actggcgcga
    13921 taacctggtg cgccaggtgc agcattcaca gctggaactg gtggccaact ttgccgatat
    13981 ctcgctacgc ctgtcgcaga ttttaaaact gaaacccggc gacgtcctgc cgatagaaaa
    14041 acccgatcgc atcatcgccc atgttgacgg cgtcccggtt ctgaccagtc agtacggtac
    14101 tctcaacggt cagtatgcgt tacggataga acatttgatt aacccgattt taaattctct
    14161 gaacgaggaa cagcccaaat gagtgacatg aataatccgg ccgatgacaa caacggcgca
    14221 atggacgatc tgtgggctga agcgttgagc gagcaaaaat caaccagcga aaaaagcgct
    14281 gccgacgcgg tgttccagca atttggcggt ggtgatgtca gcggaacgtt gcaggatatc
    14341 gacctgatta tggatattcc ggtcaagctg accgtcgagc tgggccgtac gcggatgacc
    14401 atcaaagagc tgttgcgtct gacgcaaggg tcagtcgtgg cgctggacgg tctggcgggc
    14461 gaaccgctgg atattctgat caacggttat ttaatcgccc agggcgaagt ggtggttgtt
    14521 gccgataaat atggcgtgcg gatcaccgat atcattaccc cgtccgagcg gatgcgccgc
    14581 ctgagccgtt agtgatgaat aaccatgcta ctgtgcaatc ttccgcgccg gtttctgctg
    14641 agccactgct gcaggtgagc ggcgcactca tcgccattat tgccctgatc ctcgctgctg
    14701 cctggctggt aaaacggctg ggatttgccc ctaaacgcac tggcgttaac ggtctgaaaa
    14761 ttagcgccag tgcttcgctg ggcgcgcgtg aacgggtcgt ggtggtcgat gtggaagatg
    14821 cacggctggt gctcggcgtt accgcagggc aaatcaatct gctgcataaa cttccccctt
    14881 cagcaccaac ggaagagata ccgcagaccg attttcagtc ggtcatgaaa aatttgctta
    14941 agcgtggcgg gagatcctga tgcgtcgttt gttttctgtc gcaccggtcc ttctctggct
    15001 gattacgccc ctcgccttcg cgcaactgcc gggtatcacc agccagccgc tacctggcgg
    15061 tggacaaagc tggtcgctcc cggtgcagac gctggtgttc atcacctcgt tgacgttcat
    15121 tccagcgatt ttactgatga tgaccagttt cacccgcatc attattgttt ttggtttatt
    15181 gcgtaacgcg ctgggaacgc cctccgcgcc acctaaccag gtattgctgg ggctggcact
    15241 gtttttgacc ttttttatta tgtcgccagt gatcaacaaa atttatgtcg atgcgtacca
    15301 gccattcagc gaagagaaaa tctcaatgca ggaggcgctg gaaaaagggg cgcagccgct
    15361 gcgtgagttt atgctgcgtc agacccgcga ggcagattta gggttgtttg ccagactggc
    15421 gaataccggc ccgttgcagg ggcctgaagc tgtgccgatg cgcattttgc tcccggccta
    15481 cgtgaccagc gagttgaaaa ccgcatttca gataggcttc acgattttca tccctttttt
    15541 gattatcgac ctggtgattg ccagcgtgtt gatggcattg gggatgatga tggttccccc
    15601 cgccaccatt gctctgccct ttaaactgat gctgtttgta ctggtggatg gctggcaatt
    15661 gctggtcggt tcgctggcgc agagctttta cagctagaga ggcaaaatga cacctgaatc
    15721 ggtcatgatg atgggaactg aagcgatgaa agtcgcgctg gcactggcag ccccgctgtt
    15781 gctggtagcg ctggtcacgg gccttatcat cagtattttg caggcagcca cgcagattaa
    15841 cgaaatgacg ctgtcgttta ttccgaaaat catcgccgta tttatcgcca tcattattgc
    15901 cggaccgtgg atgctcaatc tgttgctgga ttacgtccgc accttgttca ctaacctgcc
    15961 gtatatcatc gggtagcccg tacaatgatg caggagacaa gcgatcaatg gctttcctgg
    16021 ttaagcctgt acttctggcc gttactgcgc gtgctggcgc tcatttctac cgcgccaatt
    16081 ctgagcgaac gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat gatcacgttc
    16141 gccattgccc catcattacc tgccaacgat gttcctgttt tttcgttctt tgctctgtgg
    16201 ctggccgtac agcagatcct gatcggcatt gcgcttggtt ttaccatgca atttgccttt
    16261 gccgctgtgc gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc atttgcgaca
    16321 tttgtcgatc ctggcagcca tctcaatatg cccgttttag cgcgcatcat ggatatgctg
    16381 gcattactgc tgttcctgac atttaacggt catttatggt tgatttcact gctggtcgat
    16441 acctttcaca ccctgccgat tggtagcgaa ccgttgaaca gcaatgcgtt tctggcaccc
    16501 accaaagcag ggagtttgat tttccttaac gggctgatgc tggcgttacc gctcattact
    16561 ctgttgctga ccctgaatct ggcattaggt ttactgaatc gtatggcccc gcaattatcc
    16621 atttttgtta ttggatttcc attaactctg actgtcggca tctctttaat ggcggcatta
    16681 atgccgttaa ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa tttgctggct
    16741 gatattatta gtgaattacc attaatataa ttccgtaacg tttatcatgc taccctaagg
    16801 attatccgaa aaataatatc tgcgaacatc ttccaggata ctcctgtagc gaaatatttg
    16861 ttttaagctc actcacatat cgcaacattt actttaagac aattccaggc aaattataca
    16921 acactttacg ggatagtaag tccgcctgaa aaattgcgag agtggcgcat taggtgaccc
    16981 atgttgttcc gtttagtcat gatgaaatat tcaggtaagg ggaattatcg ttacgcattg
    17041 agtgagggta tgccatgtca acgattatta tggatttatg tagttacacc cgactaggtt
    17101 taaccgggta tctgttgagt agaggggtta aaaaaagaga aatcaacgac attgaaaccg
    17161 ttgatgacct tgccatagct tgtgattcac agcgtccttc agtggtgttt attaatgagg
    17221 actgtttcat ccacgatgct tctaacagtc agcgcatcaa gcacatcatt aatcaacatc
    17281 ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt tcattttgat gaatatctat
    17341 tggtcagaaa aaatttattg atcagttcta aatcgattaa accggaatct ctcgacgata
    17401 tccttggcaa tattctgaaa aaagagacaa cgataacctc gtttttaaat atgccgacgt
    17461 tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca
    17521 ttcaaatctc cgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata
    17581 ttaaacgtaa gatcaaaacg cataataaac aggttatcta ccatgtcgtc cgactgacgg
    17641 ataatgtgac taatggtatt tttgtcaaca tgcgctaaca cattctgact ggtgatttcc
    17701 caccagtcag gctgaataag attactctgc tttctcaaca aagataccgt cctgatgccc
    17761 tgcttcatta aagaaccaga tgccgagcgg gtagtcttcc agcgcaacca ggtacattgt
    17821 gccttcacta aactcctcaa ctgccagtac cactccagga cgacgcggac cgccatccgt
    17881 tttgactgtt acccgatcat tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt
    17941 agaacaattt cgtttttctg gcagcgccaa gcgcgcgcga gtgctgattt tctcgacggt
    18001 ctatacttaa gagatgctag gcggacttaa cgactggcgg caacaacaga gtaacggttg
    18061 cgaggaaaga tgatgaaaac cgcaaaagtg tacagcgata ccgcaaaacg tgaggtcagc
    18121 gtcgatgtcg atgccctgct ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc
    18181 agccagaacg attctgaaca cgttagcgtc gatggacgtg aatatcatac atggcgtgaa
    18241 ttggcggatg ccttcgaact ggatattcat gacttcagcg tctctgaagt gaatcgttga
    18301 attcacaata aaaaaatccc gaccctgagg gtgtcgggat gaaacttgct taagcaagaa
    18361 gcacttaaaa aattcgttac accaggaaat ctgatgtgtt catcacctta tctgcaattt
    18421 ttttcgctga caagaaaata ttcgcgctat gaatgattaa cgctacttat gaatagaaat
    18481 gtgacgcgcg tcaccccttg gtggctctgg tgtcaacggg atcgctcccg aaaaaatcat
    18541 ctttgattat ttgtgctgtt ttgagaatca tcctggtaag gggagtactg cgcgaccatg
    18601 ttttcaattc aacaaccact actggttttt agcgatcttg atggcaccct gctggacagt
    18661 catagttatg actggcaacc ggcagccccc tggctcagcc gtttacacga agccaatatt
    18721 cccgtcattc tctgtagcag taaaacttta gcggaaatgc tgtacttgca aaaaatgttg
    18781 gggctacaag gcttaccgct gattgcagag aatggcgcag tgatccagct tgctgaacaa
    18841 tggcaggata tagacggttt tccacgcatc atctcaggta ttagccatgg cgaaatctgc
    18901 caggttttaa atacgctacg cgagaaagaa cattttaaat tcacgacttt tgatgatgtc
    18961 gacgatgcaa ccattgccga atggacggga ttaagccgta gctaggcggc gctgacgcag
    19021 ctgcatgagg cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat ggcacaattt
    19081 atcgctcgtc tgaacgaact gggcttgcag tttatgcagg gtgcgcgctt ctggcacgta
    19141 ctggatgcct ctgccggaaa agatcaggct gccaattgga ttatcgcgac ctatcaacaa
    19201 ttgtcaggca gacgcccaac cacacttggc ctgggcgatg ggccaaacga tgcgccttta
    19261 ctggaggtaa tggattacgc ggtgattgtg aaagggctaa accgtgaagg ggtacatctg
    19321 catgatgagg atccggcccg cgtctggcga acgcagcgtg aaggaccgga aggctggcgt
    19381 gaagggctgg accatttttt ctccgcccgt taagcgttat cgctcgcgaa tacccgattg
    19441 cgcccggcct gttttgcgag gtaaagccga cggtcggcca atgactggag ttgttcaaaa
    19501 tcataatcac cggtttcctc gctgctactt acccccagcg aggcactgat gcgtatcgtt
    19561 gtactcttgg cgatcagcat ctctttttca tttaacttca ggcgaatacg ttctgcgacc
    19621 tccgcagcct gcgtcagatt cgcgcctggc agaatcacac aaaactcctc accaccgacc
    19681 cgcccggcaa cgtcctgcgc acgcaaggaa ctgctaatta atccggcagc atgagaaaga
    19741 acacggtcgc ccgcctgatg accaaagcgg tcattaatcg ctttaaaatg gtcaagatcg
    19801 acctgaatga cagaaaaagg atgttggtgc gtctgacaca atttagcgag cggacgggct
    19861 ttttcgaaca gtgcgccacg gttatataaa cgcgttaagg tgtcgtgcca cgcctgccac
    19921 tgcaacgagc tttgcagaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag
    19981 aggagtaaca tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactgccg
    20041 aaatcgccgc gcacgccttc gcttagcgta tggacgcgcg ccagcacacc gtcgaaatgg
    20101 tccagacgtt cccagctaac atagcgactg tccatacgaa tgccgccccg cgtgtcatgt
    20161 tccattgcct gcgccagcaa ggccagttca cgaggatcaa aaatattccc tgttggatgg
    20221 tcaggattgg aagaggtcaa aaatctcagc ttactgtcat agagctgata ctcaccatcg
    20281 aggtttttat cgatggcgtt tcttaaaaat tgctgcatag tacgcacggg aatactcatc
    20341 cccagcacgc cataccagta gttattactg tctaccggaa cactgacggt aacctgcggt
    20401 tcagtattgc tggcgtgttc cggttgcgaa gtgaaccagc gtaccgcgcg gtgacgattt
    20461 tctcgttgcg aatggccgat aaaccagggt tgggtgacat agccgtaata acgcgttggt
    20521 acattgcgcg taaacaaggt cggctgcgtc gaaacgtaaa atccagcacg cgagacatac
    20581 atcgcctgtt caaccatcga cgaggagttg tgcgccagtc gcagcaagta accaacttcc
    20641 agtgcagcgg taatttcatt gtcgaggctt tcattttcgc gagacaggaa attcccctcg
    20701 ctgactaatg catccgatac gccattgact ggcagggtgc gccgtcggtt gagttcgatt
    20761 tgccaggcgt gctcgtcgcg atgctgttcg aactcggtta cggcattacg cagagaggtg
    20821 aaatccagtg gcgccacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg
    20881 acgttatact gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctag
    20941 ctggagatat aggcatcttc cagcaccacc acttcacgcc aggtgagcaa ggtggaaaaa
    21001 agcaatacca cgataaagca gagattaacg acatgaccgg gccccaggcg gcgtgcgagt
    21061 tttttcaacc agctctggtt ttccattttt gtctcgtgct gcaccctgac gactcccaac
    21121 ctgctttttt atgattctgg cataacccgg caatagcgta ccgcaaaaca gcgataattt
    21181 tataatggtg gcatgataag acgcgactat cattcattgc aggcattggg acaccaggcc
    21241 ggatgtgatc cggcctggtg ttaatgatac cctttcaaac ttcttccgca gaacgactcc
    21301 cctcccccgg aacaagatcg gtttcatgaa aagcttcccg cttgaccccg taaccgtcat
    21361 accaacgaca ctcaaccata ccgctggagt atccagtgac aatcatccgc gggccgccct
    21421 ctttaaccgt aacttcctca ctaaccataa agctcatact cgcctccttt tttcgagtga
    21481 aacttgttca ccttagttga agatggcgaa ttttgcataa tagccagtgc gagtattaat
    21541 gtgcctgacc gcgcattttc gctactgctt tcactcccag caccacgatg ccgccgatga
    21601 taaatccaag aatcagattt aaaacagtcg gtaatatcat cgccaccact gcactttgct
    21661 gcccggcgat atgttcaata gcgtgatgca gcggcgcaat accatgtacc acaatcccgc
    21721 cgccgacgag gaacatcgcc agcgtgccga caatcgataa tgctttcatc agccaaggcg
    21781 cgataatcaa taacccttta cctaatgcct gcatcagcgc gctagatttt tccgccagcc
    21841 aataccctag gtcatcaatc ttaacgataa cccctaccag accgtagacg cccacggtca
    21901 ctaccagcgc gatgcctgaa agcaccagca cctgattaag caacggcgcc tctgccacaa
    21961 tccccagcgt gatggcgacg atttccgcag acaaaataaa atcggtacga atcgcccctt
    22021 taattttgtc cttttcaaac ttcagcggat cctgcgccgc cagcttctcc agacgctgct
    22081 ggctctgcgc tggatcttct ttatgtttac gcgcctccag catatgcagc actttctcta
    22141 ccccttcaaa gcagagaaac gcgccaccaa tcatcaacag tggcgtaatc gcccacggga
    22201 taaacgcact gatgatcagc gccagcggca ccagaatcac tttattgatc agcgatcctt
    22261 tcgccacgcc ccagaccacg ggaagttccc ggttggcccg cacgcctgaa acttgttgcg
    22321 cattgagcga taaatcatcc cctaatacac cggcggtttt cttcgccgcc agtttgccca
    22381 tcacagagat atcgtccagc agtgtggcga tatcatcgag caacgtcagt aaactactcc
    22441 cggccaaaag cattccctct catttttatt gttgaagggt gtaagtatga agcaaaaacg
    22501 gcagctctga tacttgcttc acaggtcaac atttatttaa caatcaagtg caattatatc
    22561 tctcgccagc agaatgattt tcacgtttac tatgaaccac ctttttattt ccgccgtgag
    22621 ggattatgcg tttccgccag ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca
    22681 tttggggctc aacctatttt gtcattcgga ttggcgtggg aagctggcct ccgttaatga
    22741 tggcgggcgt tcgattccag gcagccggta ttttattgct ggcatttttg ctactgcgcg
    22801 gacacaaact tcccccgcta cgtccgctgc tcaatgccgc gctgattggc ctgttattgc
    22861 tggctgtcgg taatggcatg gtgacggttg ccgaacatca aaatgttcct tccggcatcg
    22921 ccgccgtagt gattgcaacc gtgcccctct ttaccctgtg cttcagtcgc ctgtttggca
    22981 ttaaaacgcg caaactggaa tgggtgggta ttgccattgg gcttgccgga atcatcatgc
    23041 tcaatagcgg tggaaattta agcggcaatc cgtggggcgc gattctgatt ttaatcggct
    23101 cgattagctg ggcgtttggc tcagtttatg gctcgcgcat taccttacct gtagggatga
    23161 tggcgggtgc gattgagatg ctggcggcag gcgtggtgtt aatgatcgca tcgatgattg
    23221 cgggtgaaaa actgacggcg ctcccttccc tgtcaggctt ccttgcggtc ggctatctgg
    23281 cgctgtttgg ttcgattatc gccattaacg cttatatgta tttaatccgt aatgtcagtc
    23341 cggctctcgc caccagctac gcctacgtta acccggtggt cgcggtcttg ctgggtacgg
    23401 gactgggtgg cgaaacgctg tcgaagattg aatggctggc gctcggcgta attgttttcg
    23461 cggtggtact ggtcacgttg ggaaaatatc tcttcccggc caaacccgtt gtcgcgccag
    23521 ttattcagga tgcatcaagc gagtaaatga atcccctgcg tgtcgatctg cgcgctggca
    23581 ccttcgccgc agatccactc ttccagacgc tcggtaagcg ccgcatccgt cagcttctcg
    23641 cgcctacgta acgcacactc ccagacaatc agtacgcgcc agccgagctc ctgcaagcga
    23701 ctgatatcgc ggcgatcgcg ttcaacattt ttacctatct tctccagcca gaactcagtt
    23761 cgagtcgcag gcactttaaa cagatagcag tgatgatgat gccagaagca gccgtgggta
    23821 aatatcacgc agcgatattc atcaacgaca aaatccgggc gtccgggcag actggcgtcc
    23881 tgaacgcgaa acgcaaggcc ctgcccggtt aacaaactgg cgaggcgctt ctcaatcgcc
    23941 gtatcacgcg tggcaatcgc gcgcatattt ttgctgcggg tggccttatc gtgaacgtcg
    24001 accatgttgt gcctcttgct gacgcaacgc caccgcctgt ttgatttttg gctcaagcag
    24061 ttttgccacc gcggcaaaga ccggcacgac caccgagttg ccgaactggc gataggcctg
    24121 agtgtccgaa accggaatgc ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg
    24181 cgcgcattca cgaggcgtta accgacgtgg acgatgttgc tgattcagcg gatcgtcaaa
    24241 gtctttctca cccgtggcca tatcccaacc gcgatcgatt aaaatttccg cgccatcttt
    24301 gtaataacga gcagacagcg tacgggtgac gctttgcgga ttgttcggat aaaccattcc
    24361 ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca tatcgataga ggtacttcca
    24421 cagcaccggc gtcaggatat atttcgcctc aaccatcggg tccagcagct gcgccagcgt
    24481 cactcgctgt gcagggaaac attcgctgat atcacgcaga gtaaaatcgg ctttcagatt
    24541 cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca ggaaatgttt
    24601 accatcgatg attttcggat cgtccggccc gttatcttct gcatcggcaa cgtcatagcc
    24661 cagttcgtcc agcgtctgca tgatgatgcg gaacgttttt cccttatcgt gactcttcag
    24721 gtttttgacg ttttcgagca caaacatcgc cggacgacgc gcgtcgataa tgcgcaccac
    24781 atcaaagaac agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt
    24841 ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg aaaccggcca gtaaaacatc
    24901 gtgttcagga atgtgttgac gaatatgttc cgccgccgcc tcatcactca cgccttcttg
    24961 atgactgagg gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg ggtcgcaata
    25021 atggttggct ttataagtgc gtaccgcatg tttgttccat tcgctggtaa acacgcactg
    25081 tccaccaatc gattcaaaac cacgacggat gccgccaatt ccggcaaata gatcgataaa
    25141 gcgaaacgca taatgcggat gatgttccgg tggtttgggt aacaacgttt gcagatgggc
    25201 aaactctttc tcacttaaac ggtgccatgc cgagtcattc gccagcgcac gttttaaaat
    25261 tgccgcgctc cagtgattct cacccacacc attaagctgc gccaccagca ttttaacatc
    25321 ataaatttgc agcagtttct ccagcattgc ctgtgcggca ttcccggtgc tgtatgaatc
    25381 ggttactgat atattttcct gcatagattt aaccggccat ctgagatggc cgacaggtta
    25441 acataattac tgccgtttag ggccacgcgc tcagccagcg ggtgaaaacg catctatcac
    25501 cttgtgatcg ataccttcat tctcccccgc cagttcggca ccgagcttcg ccatcaaaca
    25561 gcgccaagct caatgcccct gaaacggcaa atacgcgcgc caggccaatc gcccccaggg
    25621 cttccagccg atccgcatcc tggacgattt tagcttccgt tgttaagggg gcgatttgcg
    25681 cgctgaaact gtgagcggca atggcatgac aaacggcctc aattttctcc gccggaaatt
    25741 gcacgaactc ttcacggagc aggcgacgcg tctcttctgc cgccaggatt gaagaacgct
    25801 gccgttgcgg atgatttttc gccaggctca cgatatcgtg aaaataacag gcggttaaaa
    25861 tcaccagcat atcgacatcg tcatctgccg ccagtttctg tgccgtagcc cagacacggc
    25921 gaaaatgaca caggtcatgc gccgcgtcct gatgttgatg atggtttttc aaccagttct
    25981 cgaactgtgc ctgccagtgt tgtaactcca tacgcgctcc gttgccaaag agtcgcaacc
    26041 ttagcagttt ttattcatag cgtctgcaac gtgcgggacg aggtcgggtg taccatgcaa
    26101 tgccgcctaa caaggcacca accagggcca taaacagaaa tccgaccaat gctcctaacc
    26161 acttccccgc ggtagatcct aaatcacttt caaatcccga cacgggggca tcggggatcc
    26221 ggctcatcac ccagatgtat cgcgccattg cccagacgaa atagccacac catgtataga
    26281 aagccc
//
LOCUS       DBKELA010000057        26073 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.057, whole genome shotgun sequence.
ACCESSION   DBKELA010000057 DBKELA010000000
VERSION     DBKELA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.057"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..2127)
                     /gene="yccS"
                     /locus_tag="ACUQR0_002304"
     CDS             complement(<1..2127)
                     /gene="yccS"
                     /locus_tag="ACUQR0_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HHV9109312.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2137..2583)
                     /locus_tag="ACUQR0_002305"
     CDS             complement(2137..2583)
                     /locus_tag="ACUQR0_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HHV9109313.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2706..4760
                     /gene="helD"
                     /locus_tag="ACUQR0_002306"
     CDS             2706..4760
                     /gene="helD"
                     /locus_tag="ACUQR0_002306"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HHV9109314.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4792..5250)
                     /gene="mgsA"
                     /locus_tag="ACUQR0_002307"
     CDS             complement(4792..5250)
                     /gene="mgsA"
                     /locus_tag="ACUQR0_002307"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HHV9109315.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5346..6008)
                     /locus_tag="ACUQR0_002308"
     CDS             complement(5346..6008)
                     /locus_tag="ACUQR0_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HHV9109316.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6181..6594
                     /locus_tag="ACUQR0_002309"
     CDS             6181..6594
                     /locus_tag="ACUQR0_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HHV9109317.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6639..6956)
                     /gene="hspQ"
                     /locus_tag="ACUQR0_002310"
     CDS             complement(6639..6956)
                     /gene="hspQ"
                     /locus_tag="ACUQR0_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HHV9109318.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7014..8204)
                     /gene="rlmI"
                     /locus_tag="ACUQR0_002311"
     CDS             complement(7014..8204)
                     /gene="rlmI"
                     /locus_tag="ACUQR0_002311"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HHV9109319.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8299..8577
                     /gene="yccX"
                     /locus_tag="ACUQR0_002312"
     CDS             8299..8577
                     /gene="yccX"
                     /locus_tag="ACUQR0_002312"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HHV9109320.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8574..8903)
                     /gene="tusE"
                     /locus_tag="ACUQR0_002313"
     CDS             complement(8574..8903)
                     /gene="tusE"
                     /locus_tag="ACUQR0_002313"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HHV9109321.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8994..9653)
                     /gene="yccA"
                     /locus_tag="ACUQR0_002314"
     CDS             complement(8994..9653)
                     /gene="yccA"
                     /locus_tag="ACUQR0_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HHV9109322.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9860..9947)
                     /locus_tag="ACUQR0_002315"
     tRNA            complement(9860..9947)
                     /locus_tag="ACUQR0_002315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9911..9913),aa:Ser,seq:tga)
     gene            10373..11491
                     /gene="hyaA"
                     /locus_tag="ACUQR0_002316"
     CDS             10373..11491
                     /gene="hyaA"
                     /locus_tag="ACUQR0_002316"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HHV9109323.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11488..13281
                     /gene="hyaB"
                     /locus_tag="ACUQR0_002317"
     CDS             11488..13281
                     /gene="hyaB"
                     /locus_tag="ACUQR0_002317"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HHV9109324.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13300..14007
                     /gene="hyaC"
                     /locus_tag="ACUQR0_002318"
     CDS             13300..14007
                     /gene="hyaC"
                     /locus_tag="ACUQR0_002318"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HHV9109325.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFPMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14004..14591
                     /gene="hyaD"
                     /locus_tag="ACUQR0_002319"
     CDS             14004..14591
                     /gene="hyaD"
                     /locus_tag="ACUQR0_002319"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HHV9109326.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14588..14986
                     /gene="hyaE"
                     /locus_tag="ACUQR0_002320"
     CDS             14588..14986
                     /gene="hyaE"
                     /locus_tag="ACUQR0_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HHV9109327.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14983..15840
                     /locus_tag="ACUQR0_002321"
     CDS             14983..15840
                     /locus_tag="ACUQR0_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HHV9109328.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15974..17518
                     /gene="appC"
                     /locus_tag="ACUQR0_002322"
     CDS             15974..17518
                     /gene="appC"
                     /locus_tag="ACUQR0_002322"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HHV9109329.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17530..18666
                     /gene="appB"
                     /locus_tag="ACUQR0_002323"
     CDS             17530..18666
                     /gene="appB"
                     /locus_tag="ACUQR0_002323"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HHV9109330.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18678..18770
                     /gene="cbdX"
                     /locus_tag="ACUQR0_002324"
     CDS             18678..18770
                     /gene="cbdX"
                     /locus_tag="ACUQR0_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HHV9109331.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18850..20148
                     /gene="appA"
                     /locus_tag="ACUQR0_002325"
     CDS             18850..20148
                     /gene="appA"
                     /locus_tag="ACUQR0_002325"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HHV9109332.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20263..22443)
                     /gene="etk"
                     /locus_tag="ACUQR0_002326"
     CDS             complement(20263..22443)
                     /gene="etk"
                     /locus_tag="ACUQR0_002326"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HHV9109333.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22463..22909)
                     /gene="etp"
                     /locus_tag="ACUQR0_002327"
                     /gene_synonym="yccY"
     CDS             complement(22463..22909)
                     /gene="etp"
                     /locus_tag="ACUQR0_002327"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HHV9109334.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22897..24036)
                     /locus_tag="ACUQR0_002328"
     CDS             complement(22897..24036)
                     /locus_tag="ACUQR0_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HHV9109335.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24082..25254)
                     /locus_tag="ACUQR0_002329"
                     /pseudo
     CDS             complement(24082..25254)
                     /locus_tag="ACUQR0_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25321..>26073)
                     /locus_tag="ACUQR0_002330"
     CDS             complement(25321..>26073)
                     /locus_tag="ACUQR0_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9109336.1"
                     /translation="RRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVF
                     REAIAEGHVATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVT
                     GQRVGDLCRMKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASS
                     SETIIASKHHDPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDK
                     FAQRLLGHKSDSMAARYRDSRGREWDKIEIDK"
BASE COUNT         6216 a         6649 c         6905 g         6303 t
ORIGIN      
        1 aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc caacttgttg
       61 tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc gctgtttcag
      121 gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc
      181 aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta actgctcccg
      241 gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca gcaagcgaaa
      301 cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg ataccaccga
      361 cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt
      421 atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt tggcctctgt
      481 ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga tgtagctcac
      541 tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgctacttc
      601 aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga acatcgttgc
      661 atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac
      721 cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg cgatacctac
      781 cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca
      841 gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta tttgaatgat
      901 ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga gggcggattc
      961 cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg ggctgtcatc
     1021 agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg attcaattgt
     1081 tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa
     1141 atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat gcgtaaaagc
     1201 gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc gtgacagttg
     1261 ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg
     1321 gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac gctcgtgaat
     1381 atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc
     1441 acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gctgaccgtt
     1501 ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag gatcaaacat
     1561 gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg
     1621 cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc
     1681 accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg ttcccaacat
     1741 agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc
     1801 gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata gccagggcca
     1861 gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag
     1921 gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc
     1981 cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc
     2041 acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccagg tatagcgttt
     2101 gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt
     2161 ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa ccggccatag
     2221 tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga ttgaaataca
     2281 ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca
     2341 gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag ccgggttcag
     2401 ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca
     2461 gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag
     2521 ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg
     2581 cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg
     2641 gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca
     2701 gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc
     2761 gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattc
     2821 ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc gagctggaat
     2881 ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc gagacgcagc
     2941 gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg agcgaaattg
     3001 cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga gaaaataaat
     3061 ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct ttgtcggcgt
     3121 tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat
     3181 gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg
     3241 aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc
     3301 cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg gccggtgcag
     3361 gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag
     3421 cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg
     3481 agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt catgcgttgg
     3541 cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg
     3601 ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc agcgaaaaga
     3661 aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag
     3721 aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc ctcgatcgct
     3781 gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg
     3841 aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct
     3901 ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt catcaggcga
     3961 ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg gttgatgaat
     4021 ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag caaaacagtc
     4081 agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc
     4141 aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc tgtgatttag
     4201 acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt attcagcaga
     4261 acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagccgtca
     4321 cgttattgga tgagagtcaa cttgatgctt tgctggataa gctctctggt tatgccaaac
     4381 cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa
     4441 aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag
     4501 ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtggt ggttttccgg
     4561 ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag gatttcccgg
     4621 acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg
     4681 cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat ctggatgtgc
     4741 cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag
     4801 acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt cgttgaaatg
     4861 cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac
     4921 cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc
     4981 ccagaagaat atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc
     5041 accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc gggaaatcaa
     5101 gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc
     5161 cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg
     5221 cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac tttcctacag
     5281 attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa
     5341 aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg cgtttgtggg
     5401 tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga
     5461 atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat catcgtggca
     5521 aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa
     5581 tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg aatcggcgtc
     5641 gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc ttcccgctca
     5701 ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc
     5761 accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat cgtcttctca
     5821 acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc accgcgcagc
     5881 agagagctgg aaaccttctt gccgtcgagt accagaagat cgacatcggt tgaaagccgc
     5941 agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg
     6001 gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct
     6061 ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt
     6121 agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata
     6181 atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc gctggtgggg
     6241 gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact cgaccagggg
     6301 tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg
     6361 tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt ccgtaattca
     6421 gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac cctctggatg
     6481 caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg gttaaatgtg
     6541 gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa ataaaaaatc
     6601 cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag acgcggggcc
     6661 tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca
     6721 tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta
     6781 tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt caccgccagc
     6841 tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga
     6901 taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc
     6961 acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg
     7021 acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat caccggatga
     7081 tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc aattgcggca
     7141 tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg
     7201 agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt ataaccacga
     7261 cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg
     7321 tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca
     7381 aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagc
     7441 gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg
     7501 gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac gtagcggcgg
     7561 gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc gtgctgaata
     7621 tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg caactcgccg
     7681 gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac atcgctgcga
     7741 tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc cgcgcgctga
     7801 tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta
     7861 atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt
     7921 tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg
     7981 tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa
     8041 taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg
     8101 ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt
     8161 aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc
     8221 gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta
     8281 aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta cgggcgagtt
     8341 cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg cttaaccggg
     8401 tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga agaagggcag
     8461 gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg
     8521 gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat ccgttaaata
     8581 cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg gcctttcggg
     8641 aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt cgccatcgct
     8701 tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc acgtacaaaa
     8761 cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc aatcaccact
     8821 gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc
     8881 tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa
     8941 caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg
     9001 cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag cgaaacatac
     9061 aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt gctggtttca
     9121 aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag atgcagagca
     9181 ggcagttgca ggaagatatt agcaatcata ccaatcagta ccaccacaat acccgccatc
     9241 agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc agagcagcag
     9301 aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac
     9361 agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg
     9421 gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata
     9481 cccaccagag tcagaatcag acccggagat ggcagcatca gcaccgtact ggcagttgcg
     9541 gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta
     9601 tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca
     9661 ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga
     9721 agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtca
     9781 ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg gtagcgttta
     9841 gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa ccctttcggg
     9901 tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca
     9961 ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc
    10021 caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac caggaattcc
    10081 ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta aagaaaaata
    10141 ggtccatttt tatcgctaaa agataaatcc acacagtttg tattgttttg tgcaaaagtt
    10201 tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa ggatgagctt
    10261 tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg tcgcttgccg
    10321 accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg atatgaataa
    10381 cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca gctttctcaa
    10441 atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac caaagattgc
    10501 ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc tggaatgtac
    10561 ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg tcatcctttc
    10621 cctgatttcc ctcgattacg acgataccct gatggctgcc gccggaaccc aggcggaaga
    10681 agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag tagaaggtaa
    10741 tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt ttattgaaaa
    10801 actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct gcgcgtcctg
    10861 gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg acaaagtcat
    10921 caccgacaaa cccattatca aagtacctgg ctgcccgccg atcccggatg tgatgagcgc
    10981 catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa tgggtcgtcc
    11041 actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc acttcgacgc
    11101 cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt gcctgtacaa
    11161 aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct ggaatgatgg
    11221 cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa atggtttctg
    11281 ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta ctcattccac
    11341 cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg gtgtgcacgc
    11401 agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag aaaccgaaca
    11461 tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca gggatacacc
    11521 atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga aggccacatg
    11581 cgctgtgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc ctgcggcacc
    11641 atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc gtgggcgttc
    11701 gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt ttacgccatc
    11761 gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa cattatgctg
    11821 gcaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg gatggactgg
    11881 atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact ggcgcaaagt
    11941 ctctcctctt ggccgaaatc atcccctggc tatttcttcg acgtacaaaa tcgcctgaag
    12001 aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg gcacccgcag
    12061 tacaaactgc cgccagaagc caacctgatg ggctttgccc actatctcga agctctcgat
    12121 ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc gcatccaaac
    12181 tggattgtcg gtgggatgcc ttgcgccatt aacattgacg aaagcggcgc ggtcggggcg
    12241 gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc agacttcatt
    12301 aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc gtggagcgaa
    12361 atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc ggatattgcc
    12421 aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa cggcgacttc
    12481 aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt tgtcgatcac
    12541 gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg cattaccgac
    12601 ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca gctgaatgaa
    12661 caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat ggaagtgggg
    12721 ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt tgagtcggtc
    12781 gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac gttgggccgc
    12841 attttgtgcc gcgcgcacga agcgcagtgg gccgcaggta agttgcagta tttcttcgac
    12901 aagctgatga ccaacctgaa aaacggcaat ctcgctactg cttccactga aaaatgggaa
    12961 cctgcaacct ggccgacaga gtgccgtggc gtcggcttta ccgaagcgcc gcgcggagcg
    13021 ttaggccact gggccgccat tcgcgatggc aagattgatc tctaccagtg cgtggtgccg
    13081 accacctgga acgccagtcc gcgcgatccc aaagggcaga ttggcgctta tgaagcggcg
    13141 ctgatgaaca ccaaaatggc gatccccgag caaccgctgg agatactgcg tactctgcac
    13201 agctttgatc cgtgcctcgc ctgttcaact catgtgctgg gcgacgacgg tagcgagctg
    13261 atctccgtgc aggtgcgtta acagcgaagg agaatcatca tgcaacagaa aagcgacaac
    13321 gttgtcagcc actatgtctt tgaagcacca gttcgtatct ggcactggct gacggtgtta
    13381 tgcatggcgg tgttgatggt caccggttac tttatcggca agccgctacc ttccgtcagt
    13441 ggcgaggcga cgtatctgtt ctatatgggt tacatcaggt taattcactt cagcgccggg
    13501 atgattttta ccgtggtctt gctgatgcgg atctactggg cttttgttgg caatcgttac
    13561 tcccgcgagc tgtttatcgt gccggtatgg cgtaaaagct ggtggcaggg cgtgtggtat
    13621 gaaatccgct ggtatctgtt tctggcaaaa cgtccgagtg ccgatatagg ccataacccc
    13681 atcgcccagg cggcgatgtt cggctatttc ccgatgtcgg tctttatgat catcaccggt
    13741 tttgcgctgt acagcgaaca cagccagtac gctatttttg cgccgttccg ttatgtggtg
    13801 gaatttttct actggacggg tggcaactca atggacattc acagctggca tcggctgggg
    13861 atgtggctga ttggggcgtt tgtgatcggt catgtctaca tggcgctgcg tgaagacatc
    13921 atgtccgacg acacggtgat ctccaccatg gtcaacggct accgtagcca caaatttggc
    13981 aaaataagta acaaggagcg ttcatgagcg agcaacgcgt ggtggtcatg gggctgggca
    14041 acctgctgtg ggccgatgaa ggcttcggcg tgcgggtggc ggaacggctg tatgcccatt
    14101 accactggcc cgagtatgtg gagattgtcg atggcggtac tcagggactg aacttgctgg
    14161 ggtatgtcga aagcgccagc catctgttga ttctcgatgc cattgactac gggctggaac
    14221 ctggaacgct gcgaacctat gccggagaac gcattccggc ctatctcagc gcgaagaaaa
    14281 tgagcctgca tcagaacagt ttctccgaag tgctggcgct ggcagatatc cgcggacatc
    14341 tgccagcaca tattgccctc gtcgggctgc aacccgcgat gctcgacgac tacggcggca
    14401 gcctgagcga actggcacgg gagcaactgc ccgctgcgga acaatcggcg ctggcgcagt
    14461 tggctgcgtg gggaagtgtg ccgcaaccgg ctaatgaatc gcgttgtctc aattatgact
    14521 gtctgtcgat ggaaaattac gaaggcgttc gcttgcgcca gtaccggatg acacaggagg
    14581 agcagggatg agcaacgaca cgccatttga tgcgttgtgg caacgaatgc tggcgcgcgg
    14641 ctggacgccg gtcagtgaat cccgtcttga cgactggctt acgcaagcgc cagacggcgt
    14701 ggtgttatta agcagtgacc cgaaacgcac gccagaggtc agcgataatc cggtaatgat
    14761 tggcgaatta ctgcacgagt ttcccgacta tacatggcag gtggcgattg ctgaccttga
    14821 gcagagcgaa gccatcggcg atcgctttgg cgcctttcgc tttcctgcca ctttagtgtt
    14881 taccggcgga aactatcgcg gcgtgctgaa tggtattcac ccgtgggcgg aactgataaa
    14941 cctgatgcgc gggcttgtcg aaccgcagca ggagcgtgcc tcatgagcga aacttttttc
    15001 catctgctgg ggccaggaac gcaaccgaac gatgacagtt tcagcatgaa tccactgccg
    15061 atcacctgtc aggtgaatga tgaaccgagt atggcggcct tggagcaatg tgctcacagc
    15121 ccgcaggtga ttgcgctgtt aaacgagtta caacatcaac taagcgaacg ccagccgccg
    15181 ttgggcgagg tgctggcagt cgatctgtta aatctcaacg ccgacgatcg tcactttatc
    15241 aatacgcttc tcggggaagg ggaagtgtca gtgcgcattc agcaggctga cgacagtaaa
    15301 agtgaaatac aggaggcgat cttctgcgga ttatggcggg tgcgcagacg tcgcggcgaa
    15361 aagttgctgg aggacaaact ggaggctggc tgcgcgccgc tggcgttgtg gcaggcggca
    15421 acgcaaaatc tcttgccgac agattcgctg ttaccgccgc ccattgatgg cctgatgaat
    15481 ggcctaccgt tggcgcatga gttactggca catgtacgta accccgacgc gcagccgcac
    15541 agcattaatc tgacgcaatt acccatcagc gaggctgatc ggctttttct ctcgcgtctc
    15601 tgtgggccgg gaaatattca gattcgtacc attggctatg gcgagagcta tatcaacgcc
    15661 acggggttac gccatgtctg gcatttacgc tgtacggaca ccttaaaagg cccgttactg
    15721 gaaagttatg aaatctgccc aataccggaa gtggtgctgg cagcgccaga agatttggtc
    15781 gactctgcgc agcggcttag cgaggtatgt cagtggctgg cggaagctgc accgatgtaa
    15841 aaagacggta agtatcgctt tcagtcttat gaatatcgca atcggcgaat acctctggtc
    15901 gtagagtttc aggataaaga gggagatcta acattatcgg gttatttttc tctcttcgcc
    15961 tacaggagtg cgcatgtggg atgtcattga tttatcgcgc tggcagtttg ctctgaccgc
    16021 gctgtatcac tttttatttg taccccttac cctggggctg atttttttgc tggctatcat
    16081 ggaaaccatt tacgtggtca ccggcaaaac aatctaccgc gatatgacgc gcttctgggg
    16141 taagctcttc ggtatcaatt ttgctcttgg cgtggctacc ggcctgacca tggagtttca
    16201 gtttggtact aactggtcat tctattccaa ctatgtgggc gatatttttg gcgcaccact
    16261 ggcgatggaa gcattaatgg ccttcttcct cgaatccacc tttgtcgggc tgttcttctt
    16321 cggctggcaa cggctgaata aataccagca cctgctggtg acgtggctgg tggcgttcgg
    16381 ttcaaatctc tctgcattgt ggatattgaa tgccaacggt tggatgcaat acccgaccgg
    16441 tgcgcatttt gatatcgaca ccctgcgtat ggagatgacc agcttcagcg agctggtctt
    16501 taacccggtc agccaggtga aatttgtgca caccgtcatg gcgggctacg tgaccggggc
    16561 catgtttatt atggcgatca gcgcctggta tttactgcgc ggacgggagc gcgatgtcgc
    16621 attacgctcg tttgccatcg gttccgtctt cggtactctg gcgattatcg gtaccctgca
    16681 actcggagac agttctgcgt atgaagtcgc gcaagtacaa ccggtaaaac tggcggcgat
    16741 ggaaggggag tggcaaacgg aacctgctcc tgcaccgttc catgtggttg cctggccgga
    16801 acaggatcaa gagcgtaacg cctttgccat caaaattccc gcgctgctag ggatcctcgc
    16861 cactcactca ttagataaac ccgtgccggg tctgaagaat ttgatggctg aaacctaccc
    16921 acgcttgcaa cgcggacgta tggcctggct gttaatgcag gaaatatctc aaggcaatcg
    16981 tgagccgcat gtgttgcagg cattccggga gctggaaggc gaccttggtt acggcatgtt
    17041 gctctcccgc tatgcgccgg atatgaatca tgtcacagcc gcacagtacc aggcggcgat
    17101 gcgtggcgcg atacctcagg ttgcgccggt attctggagt ttccgcatca tggtgggctg
    17161 tggttccctg ctgctactgg tgatgctgat tgcgcttgtc cagacgctgc gtggcaaaat
    17221 cgaccagcat cgctgggtgc tgaaaatggc gctctggagt ttgccgttgc cgtggattgc
    17281 gattgaagcc gggtggttta tgaccgagtt tggtcgtcag ccgtgggcga tacaggacat
    17341 cttaccgaca tactccgcgc actccgcttt aaccacagga caactggctt tctcactgat
    17401 catgatcgta gggctttaca ccctgttctt aatcgccgaa gtctacctga tgcagaaata
    17461 tgcccgtctg gggccgagcg cgatgcagag tgaacaaccg acgcagcaac aggggtaaag
    17521 gagaaaatca tgtttgatta tgaaacattg cgcttcatct ggtggctgct gattggcgtg
    17581 atcctggtgg tctttatgat ctccgacgga tttgacatgg ggatcggctg tttgctgccg
    17641 ctggtggcgc gtaatgatga tgaacgccgg atagtgatta atagcgttgg tgcgcactgg
    17701 gaaggcaatc aggtctggtt gatcctcgca ggtggggcat tatttgccgc ctggcccaga
    17761 gtgtatgcag cggcgttttc cggcttttat gtggcgatga tcctggtgct gtgctcgctg
    17821 ttcttccgtc cgctggcctt tgattatcgc ggaaaaattg ccgatgcacg ctggcgtaaa
    17881 atgtgggatg ccggtctggt catcggcagt ctggtgccgc cggtagtctt cggtatcgcc
    17941 ttcggcaact tgttgctcgg cgtgccgttt gccttcacac cgcaattacg cgtggagtat
    18001 ctcggcagct tctggcaact gctgacgcca ttccctttat tgtgtggttt gctcagcctt
    18061 gggatggtga ttttgcaagg tggcgtctgg ttacaactga aaactgttgg tgtgattcat
    18121 ctgcgttcac agctggcgac taaacgcgct gcactgttgg tgatgctgtg ctttttgctg
    18181 gcaggttact ggctgtgggt cggtattgat ggctttgtac tgctcgccca ggatgctaac
    18241 ggtccttcca atccgttaat gaaactggtg gcagtgctac ctggtgcctg gatgaataat
    18301 ttcgtcgaat cgcccgtttt gtggatcttc ccgctgctgg ggttcttctg cccattgctg
    18361 acggtgatgg cgatttatcg tggtcgcccg ggttggggat ttttgatggc atcattgatg
    18421 caatttggcg tgattttcac ggcaggcatc acgctgttcc cctttgtcat gccgtcaagt
    18481 gtgagtccaa tctccagcct gacgttgtgg gacagcactt ccagtcagct gacgctgagc
    18541 attatgttgg taatcgtgct gatatttttg cccattgtgt tgctctacac tctctggagc
    18601 tactacaaaa tgtggggacg catgacaaca gaaactctcc gccgtaacga aaacgagttg
    18661 tactaaggag caggaacatg tggtatttac tttggttcgt cggcattttg ttgatgtgtt
    18721 cgctctccac ccttgtgttg gtatggctgg acccgcgtct gaaaagttaa cgaacgtagg
    18781 cctgatgcgg cgcattagca tcgcatcagg caatcaataa tgttagatat gaaaagcgga
    18841 aacatatcga tgaaagcgat cttaatccca tttttatctc ttctgattcc gttaaccccg
    18901 caatctgcat tcgctcagag tgagccggag ctgaagctgg aaagtatggt gattgtcagt
    18961 cgtcatggtg tgcgtgctcc aaccaaggcc acgcaactga tgcaggatgt caccccagac
    19021 gcatggccaa cctggccggt aaaactgggt tggctgacac cgcgcggtgg tgagctaatc
    19081 gcctatctcg gacattacca acgccagcgt ctggtagccg acggattgct ggcgaaaaag
    19141 ggctgcccgc agtctgctca ggtcgcgatt attgctgatg tcgacgagcg tacccgtaaa
    19201 acaggcgaag ccttcgccgc cgggctggca cctgactgtg caataaccgt acatacccag
    19261 gcagatacgt ccagtcccga tccgttattt aatcctctaa aaactggtgt ttgccaactg
    19321 gataacgcga acgtgactga cgcgatcctc tgcagggcag gagggtcaat tgctgacttt
    19381 accgggcatc ggcaaacggc gtttcgcgaa ctggaacggg tgcttaattt cccgcaatca
    19441 aacttgtgcc ttaaccgtga gaaacaggac gaaagctgtt cattaacgca ggcattacca
    19501 tcggaactca aggtgagcgc cgacaatgtc tcattaaccg gtgcggtaag cctcgcatca
    19561 atgctgacgg agatatttct cctgcaacag gcacaaggaa tgccggagcc ggggtgggga
    19621 aggatcaccg attcacacca gtggaacacc ttgctaagtt tgcataacgc gcaattttat
    19681 ttactacaac gcacgccgga ggttgcccgc agtcgcgcca ccccgttatt ggatttgatc
    19741 atggcagcgt tgacgcccca tccaccgcaa aaacaggcgt atggtgtgac attacccact
    19801 tcagtgctgt ttattgccgg acacgatact aatctggcaa atctcggcgg cgcactggag
    19861 ctcaactgga cgcttccagg tcagccggat aacacgccgc caggtggtga actggtgttt
    19921 gaacgctggc gtcggctaag cgataacagc cagtggattc aggtttcgct ggtcttccag
    19981 actttacagc agatgcgtga taaaacgccg ctgtcattaa atacgccgcc cggagaggtg
    20041 aaactgaccc tggcaggatg tgaagagcga aatgcgcagg gcatgtgttc gttggcaggt
    20101 tttacgcaaa tcgtgaatga agcacgcata ccggcgtgca gtttgtaatg cataaaaaag
    20161 agcattcagt tacctgaatg ctctgaggct gatgacaaac gaagaactgt ctaatgcgta
    20221 gaccggaaaa ggcgttcacg ccgcatccgg tcactttcag ttttactctt tctcggagta
    20281 actataaccg taatagttat agccgtaact gtaagcggtg ctggcgcgtt taatcacacc
    20341 attgaggatt gtgcctttaa tattaacgcc agcctgttcc agacgctgca ttgacaagct
    20401 cacctctttg gcggtgttca agccaaaacg cgcaaccagc aggctggtgc cgacagagcg
    20461 tcccacgacc gcagcattgc tcaccgccag catcggcggc gtatcgacaa tcaccagatc
    20521 gtaatggtcg ttcgcccatt ccagtaattg acgcatccga tcgcgcatca gcagttcaga
    20581 cgggttaggt ggcacctgac cgcgagtaat cacatcaaag cctcctttgc caaaatgctg
    20641 gatcactttg ttgagctcat ctttacctgc cagatattcc gacaagccat gttcattact
    20701 cacggtaaac aggttatgcg aataaccacg gcgtaagtcg gcatcaataa ataacacttt
    20761 ttgatcggac tgggcgatca ccgctgccag agttgaactg acaaacgttt taccactgtc
    20821 tggcgtcgca ccggtgatca tcagaatgtt attctcagtc tccatcatag cgaaatgcag
    20881 actggttcgt agcgcacgta cggcttccac agcagaatcc gccgggttat ccaccgccag
    20941 gaaggggata tttttagtac gatggcgctg ctgattagaa aataaatttt tcttacgcag
    21001 acgggtgcgt ttatccagcc actcggacat tgggatagtg gaataaacgc tgatgccgtg
    21061 ctcttccagt tgttccgggg cttctacacc acgacgcaac atcgcacgcg ccagcacggc
    21121 actcaccgaa ataaacaggc caagaataaa accaagcacc acattcaacg cttttttcgg
    21181 tttcactggc tgcggctgag tgactgccgg gtcgataatc cgcacgttac caatggcact
    21241 ggatttcgaa atactcaact cctgctggcg gttaagtaat tgcagatata ccgcacggcc
    21301 cgcttctacg tcacgactta aacgcaacac ttcctgttgg gtggaaggca ttgccgatac
    21361 ccgcttattc aggcgtttgc gttcttgctc cagcgtctgg cgtttttcca gcagcgcacg
    21421 ataagttggg tgatctttct tatacagctg ggagatctct gcctcgcgga aggtcagctc
    21481 attgagttga ttatcaacgt tcacaatctg ctcaagaacg gctttggctt ccaggttaag
    21541 gtcaaccgaa tcgcgctgct ggcgataaac gttgagtttt tcttccgcct ggtccagctc
    21601 gctgcgcact tcaggtaact ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc
    21661 ctggcgagcg atattctgtt gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat
    21721 caactgggga tcatcaccag tcatggtaag ttccagcatc ccgctttctt tactgcgttc
    21781 gctaacggta aaggtttcct gcaatgcgtt aatcgcttcc agttcggtac gctggctcag
    21841 gacaaactgt gttcctggtt tggccttaat gtccgcgata gtcagcgcaa cgccatcttt
    21901 ttccagacgc tggccgacca taccattgac ggtgaactct tcaccttcca gtgtatagtg
    21961 gccgttttcc ccaaccgtga gtgtcagttg ctgatcctga ccattcagtt gtggaatatg
    22021 catccagctg atcgccagct cacctagttt ttctttggtt aatctcgccc agccgcgacc
    22081 cacaatcgga aaatacttct gctcaactat gtcgcgcaga ttcagttcag caatggtttt
    22141 accgagaatc atgcgcgatt gcagcagttg gatctccggt gcagactcgg gcgatgagtt
    22201 agggatcata tcgctcaggc cgctgagaat ggcgttgccc tgtttttgct caacctggac
    22261 cagagtatct gcctgataaa ttggtgtgct taacagcgag taagcgacag cgatcagcgt
    22321 gaataacgcg gtcacgctga taataaactt acggtgatcc cataactcgc cgaccagacg
    22381 aagcagatcg atctcatttt cctgagtgct gcctggtggc gtattcatat ttttagttgt
    22441 catcgtattt tataaactca aattaccggc tgaggcgctt cgcccattcc tgactggcgc
    22501 gctccaacat accgtagaca tgttcaaatg cgtcctgact tttacgatag ggatccggga
    22561 tctctttctg ttccagccat tgcccaaaca gcattgtctt cccgcggacc tcgggagcaa
    22621 ttgcggtaac ctgagcaata tgttccgact ccattgccag aatcagatcg taatttcgtg
    22681 ccatctcagc agtgagctta cgtccggcat gtccttcaag agatacgcca tgattagcgg
    22741 caacatctgc cgctgtcgca tctgcagggt gttttaccag accatgaaca cctgccgatt
    22801 tgacttttac gcccggcaga cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa
    22861 tgttgccggt acaaaccact aggattgagt taaattttag ttgggccatg tttgaatcca
    22921 ccgtgaagtc tctgtcaggt catgcacccc ggaaatcgtc ggtaccagca gagaaatcac
    22981 acggttccaa cgagccagcg gcgcggtagt gacataaaca atgtcgtaag gttcaagctg
    23041 gaattccgtt ccgagtatca ttgccgatgc gtccttcgca ttcagctgat aaatattggc
    23101 gattttgcca ttctgcttgt tttgcgttgc acgaataaca aaaatccccg tcgcatcagc
    23161 gacatcctga ttcataccct cagcgttacc cagcgcttct gccagggtca taccgctgcg
    23221 gtccattttc agcgtgctct gtttacccac ttcacccatc acgaagactt tcagatcatc
    23281 gttgcgcgga ataaataaaa tatcgcccgg gtgcagcagt ttgttctggc gcaagtcgcc
    23341 acgctgcatc agcgcataga ggttcacttt ggtctttacg ccattctggg tgagcaccac
    23401 gttgcgccag tctgcatctg cggtcagacc gcccgcggcg ttaatcgcat ccatgatggt
    23461 cagaggaata ttggtaatcg gctgctgacc cgatttgctg acttcgccgg tgacgtacgc
    23521 tttttgcgat cggaacgccg cgacgctgac atcgacttgc gggctttcaa tgactgaatc
    23581 gagacgcgct gtaatctcgt tgcgtacctg ggtaagtgtt ttcccggcca ctttcaggcg
    23641 accaatatag gggtagaaaa tcgcaccatc ggcgttaacc cagttgccgg tatcactggc
    23701 gctacggtat tgccctgcgg gggtggttag ttccgggtga tcccagaccg taaccatcaa
    23761 cacatcacca atcccgatac gatattcata gtttgcgatt tgctgctcca gctccgggtt
    23821 agcctgcgac ataatcggtt tggcgcgcag ctgatcaatc aatcctggcg tgacgggata
    23881 gatattgacc attttgtcca ggtcatactg attgtcaggt agttcaatga catccttgtt
    23941 actggtcgat aaattctgac caggaacaag ggtacatgct gtcaaagaca gaacagccaa
    24001 tgtcagtacc gaaaacttaa aaatgttctt tttcattggt ttatatttat cagtcaatgt
    24061 aatgttatga attacgtcag gttattgtcc aaccggtact tctcgctctg ccgtcattgg
    24121 atcgagctgg tatttgcgtg acagcatttg tccaccatca cgcgtcaatg gtgtccacga
    24181 aaccaccgcg cggttgcggt taggtccaat ggtcatcaag tcgaacggaa tcgagatata
    24241 aaaaccttta ctaaagccgc cttcgccgta gtcatcttta gatacgttac tgattgccgc
    24301 ccatacccct accgccacgc cgctgtcaaa gcgtttggcg acgtcgatcg ttgcgccttt
    24361 atcttttgcc agatattgcc caacgctaag tttcatcagt acgccattga gcgtcggcgg
    24421 gttccagtaa gccgtcacga aacctgtggg cgtggaataa tcggtgaagc gcatcatgtt
    24481 gtcccagtca cgctgcttca cgtagttaac gtccacaccc agcgcccagc tggcatctag
    24541 cgggcgataa agaagctcgg aaccgacacc ggcatacatc gtttccagat aaccgccata
    24601 cacctggcca tagaaaccat tgcctaagtc ggcaaagtaa ttcgcttgca agttgttgag
    24661 ataaacgtca ttgcgaacgt aatcacggat atgcgtgcgc acgcggggca gggtagagtc
    24721 cgcgggtaac agtgaagact taaacttgtc gtagttgttg taaatattgg tgaaaatacc
    24781 gtcatcaagc agcaggtggt cggtaaacca gtagcgggcg ctggacatca gccccagctg
    24841 gaacatatag aaatcttccg ggccgcccag cgactgtgaa agtgttgggt tgaaagagta
    24901 gctaaagcga tcttcacgaa tacggtagcc ccgaccaaag gcagaaagat cttctgcttc
    24961 aacacgttgt tgttgcagtt gctctgattt accaggcgct gtgccggcca gctgtttgcg
    25021 caggctggct acgtcggttt cggtagtcac catcgccata tgctcgcgct tttgcgtcac
    25081 gctaatcttc tcaacgcctt gcggcaggtt attcaccaga atccggttgg cacgatcgac
    25141 cgcttcgcga gagtcacggt atttgtattg ctgaccagac atatacagcg tcttatcgcg
    25201 tagctgaatt tctggcgcgt caaagcccgc gttatacttc aggtgaataa gttgtatatt
    25261 taaaatctct ttgactatca atgaattagt ataagaaggt cattattagt caaaataaaa
    25321 tcatttgtcg atttcaattt tgtcccattc ccgtccacgg ctgtccctat accgcgccgc
    25381 cattgaatct gatttatgtc cgagaagacg ttgagcaaac ttatcgccaa tctgattacg
    25441 gtatagcctc gccgacaggc tacgcagttc atggaatgtt ggcgggtctc catcaaatga
    25501 gagtcctgat gcatttctcg cctttgtaaa atacttcgat actgttttcg gggaaagcgg
    25561 atcgtgatgc tttgatgcga ttatagtttc actgctgctg gcctccctgc atttctgtag
    25621 tgtatcagcc aatgagatat tgagcgcgtc aatcgttagc gtcagcggaa tggcgagctt
    25681 agcccctgtt ttgctctgtt caatgtgaag atggttgtcg tttatgtctg accatttcat
    25741 tctgcacaaa tcgccgactc tctgccctgt aacgacagcc aaatccattg ctagcctcag
    25801 ccagattggg agaggttcgg ctgcatggta aatcgcgaca tactcattag ctgtcagcct
    25861 tgagcgcctt acttctgact ttgctgtgcg ggttgctgtt accggattcg ttgctacatg
    25921 cccctcggct attgcctcac gaaaaacgtc aacaagggtt gacctgatta attttgcgga
    25981 agccgcttta ccttctgcta cgtaggtgtt tagcattgct gccacctctt tcgttgatat
    26041 gtcagcgagc ggtttgtccg gcaattttct tcg
//
LOCUS       DBKELA010000058        24600 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.058, whole genome shotgun sequence.
ACCESSION   DBKELA010000058 DBKELA010000000
VERSION     DBKELA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.058"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..374)
                     /locus_tag="ACUQR0_002331"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="ACUQR0_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="ACUQR0_002332"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="ACUQR0_002332"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HHV9109337.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="ACUQR0_002333"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="ACUQR0_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HHV9109338.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="ACUQR0_002334"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="ACUQR0_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HHV9109339.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="ACUQR0_002335"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="ACUQR0_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HHV9109340.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="ACUQR0_002336"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="ACUQR0_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HHV9109341.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="ACUQR0_002337"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="ACUQR0_002337"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HHV9109342.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="ACUQR0_002338"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="ACUQR0_002338"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HHV9109343.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="ACUQR0_002339"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="ACUQR0_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HHV9109344.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="ACUQR0_002340"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="ACUQR0_002340"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HHV9109345.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="ACUQR0_002341"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="ACUQR0_002341"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HHV9109346.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="ACUQR0_002342"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="ACUQR0_002342"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HHV9109347.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGITSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="ACUQR0_002343"
     CDS             10736..11161
                     /locus_tag="ACUQR0_002343"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HHV9109348.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="ACUQR0_002344"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="ACUQR0_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HHV9109349.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /gene="selO"
                     /locus_tag="ACUQR0_002345"
     CDS             12185..13621
                     /gene="selO"
                     /locus_tag="ACUQR0_002345"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HHV9109350.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="ACUQR0_002346"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="ACUQR0_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HHV9109351.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="ACUQR0_002347"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="ACUQR0_002347"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HHV9109352.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="ACUQR0_002348"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="ACUQR0_002348"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HHV9109353.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="ACUQR0_002349"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="ACUQR0_002349"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HHV9109354.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="ACUQR0_002350"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="ACUQR0_002350"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HHV9109355.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="ACUQR0_002351"
     CDS             complement(20454..20747)
                     /locus_tag="ACUQR0_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HHV9109356.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="ACUQR0_002352"
     CDS             complement(20744..22033)
                     /locus_tag="ACUQR0_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHV9109357.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="ACUQR0_002353"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="ACUQR0_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="ACUQR0_002354"
     CDS             complement(23047..23811)
                     /locus_tag="ACUQR0_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HHV9109358.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24600
                     /locus_tag="ACUQR0_002355"
     CDS             24127..>24600
                     /locus_tag="ACUQR0_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HHV9109359.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6095 a         5940 c         6463 g         6102 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaataaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
//
LOCUS       DBKELA010000059        24180 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.059, whole genome shotgun sequence.
ACCESSION   DBKELA010000059 DBKELA010000000
VERSION     DBKELA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.059"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(22..594)
                     /locus_tag="ACUQR0_002356"
     CDS             complement(22..594)
                     /locus_tag="ACUQR0_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HHV9109360.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="ACUQR0_002357"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="ACUQR0_002357"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HHV9109361.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACUQR0_002358"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACUQR0_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HHV9109362.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACUQR0_002359"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACUQR0_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HHV9109363.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="ACUQR0_002360"
     CDS             complement(3556..5337)
                     /locus_tag="ACUQR0_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HHV9109364.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="ACUQR0_002361"
     CDS             complement(5330..7102)
                     /locus_tag="ACUQR0_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HHV9109365.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACUQR0_002362"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACUQR0_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HHV9109366.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACUQR0_002363"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACUQR0_002363"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HHV9109367.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8661..10353
                     /locus_tag="ACUQR0_002364"
                     /pseudo
     CDS             8661..10353
                     /locus_tag="ACUQR0_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10418..11113
                     /gene="queC"
                     /locus_tag="ACUQR0_002365"
     CDS             10418..11113
                     /gene="queC"
                     /locus_tag="ACUQR0_002365"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HHV9109368.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="ACUQR0_002366"
     CDS             complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="ACUQR0_002366"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HHV9109369.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11657..12028)
                     /locus_tag="ACUQR0_002367"
     CDS             complement(11657..12028)
                     /locus_tag="ACUQR0_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HHV9109370.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="ACUQR0_002368"
     CDS             complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="ACUQR0_002368"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHV9109371.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14242..14532)
                     /gene="hupB"
                     /locus_tag="ACUQR0_002369"
     CDS             complement(14242..14532)
                     /gene="hupB"
                     /locus_tag="ACUQR0_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HHV9109372.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14723..17077)
                     /gene="lon"
                     /locus_tag="ACUQR0_002370"
     CDS             complement(14723..17077)
                     /gene="lon"
                     /locus_tag="ACUQR0_002370"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HHV9109373.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="ACUQR0_002371"
     CDS             complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="ACUQR0_002371"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HHV9109374.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="ACUQR0_002372"
     CDS             complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="ACUQR0_002372"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HHV9109375.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19535..20833)
                     /gene="tig"
                     /locus_tag="ACUQR0_002373"
     CDS             complement(19535..20833)
                     /gene="tig"
                     /locus_tag="ACUQR0_002373"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HHV9109376.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="ACUQR0_002374"
     CDS             complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="ACUQR0_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HHV9109377.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21799..22377
                     /locus_tag="ACUQR0_002375"
     CDS             21799..22377
                     /locus_tag="ACUQR0_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HHV9109378.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22421..23896
                     /gene="ampG"
                     /locus_tag="ACUQR0_002376"
     CDS             22421..23896
                     /gene="ampG"
                     /locus_tag="ACUQR0_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HHV9109379.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6664 c         5827 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcattcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 taatattgtt ccgggtttat atgcgattgc tcaaccgtct taaccagtat caacgtctgt
     8701 ggcaaccttc agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc
     8761 gaacgccatg ttcgtacgct gttgcgtcag gcacaggagg cgggatggct ggagtggcag
     8821 gcgcagtcag gacgcggaaa gcgcggacaa ttacgctttc tggtcacgcc ggaatcgcta
     8881 cgcaatgcga tgatggaaca ggcactggaa accggaaagc agcaagatgt gctggagctg
     8941 gcgcaactgg ccccaggtga gctgcgcact ctgttacagc catttatggg cggacaatgg
     9001 caaaacgata cacccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaacca
     9061 ggctttttgc ccggccgtgc cgagcagcat ctcgccgggc agatattttc cggcctgacc
     9121 cgcttcgata ataatactca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct
     9181 actgacaggt tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat
     9241 gcagtaaaag cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg
     9301 gatcaattat ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc
     9361 tttttacatc gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg
     9421 gcgcatccgc aattcccact gatcggcact ggtccttttc gcttaacaca attcacagcg
     9481 gagctggtgc gcctggagag ccatgattat taccatttgc gtcatccgct gcttaaagcg
     9541 gttgagtact ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat
     9601 cccgtgcaaa tcaccatcgg caaaccggag gagctgcaac gggtcagcca agtcagtagt
     9661 ggcatcagtt taggtttttg ctatttaacg ttgcgcaaaa gtccccgact ctccctctgg
     9721 caggcgcgaa aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc
     9781 ggagaaaacc tgatcaccgc cagtcatgca ttactgctag gctggactat tccgcactgg
     9841 caagtaccgg atgaagtcaa actaccgaaa accttgacgc tggtttatca cctaccgata
     9901 gaacttcata ccatggcaga acgcctacag gcgacactgg cagcggaagg ctgtgaactc
     9961 acaattattt ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac
    10021 ctcatgatgg gcgacagatt aattggcgaa gcaccggaat atactctgga gcaatggcta
    10081 cgttgcgatc cactatggcc acatgttttc gacgctccag catatgcaca tctgcaatcg
    10141 acgctggacg cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctgaaagcg
    10201 gtttttagcc agttaatggc agacgcgacg ctgacgccgc tgttcaacta tcactatcgc
    10261 attagtgccc ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt
    10321 accgaagcct ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg
    10381 ttaccataac gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt
    10441 cagtggaggc caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt
    10501 ccattgcgtg acgttcgatt acggtcagcg acatcgcgca gaaatcgacg tggcacgcga
    10561 actggcgctg aaactgggag cacgcgcgca taaggttctt gatgtaacgc tgctcaacga
    10621 gctggcggtc agcagcctga cacgtgacag cattccggtg cctgattatg aacctgaagc
    10681 cgatggcatc ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga cgctggcggc
    10741 aatatatgcg tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg aaacggattt
    10801 ctccggctac ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt
    10861 gggcatggcg aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac
    10921 ctgggcgctg gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg
    10981 ctataacggc attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa
    11041 cggtttgaat cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac
    11101 cgggttgagg taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa
    11161 acgtttactt aaccatctgc tccagctttt cgcgcaattc cccttccaga gctaatgctt
    11221 tctgcgtttt aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt
    11281 ccggctccag tgtaatgacc tggtttaaga tgccgctttt accgtttaat tgctgcaact
    11341 gactggtaat agttaacagg tcgcttaata ccgctgggcg acgatagtta atattgatat
    11401 tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca
    11461 ccccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg acgtgctggt
    11521 aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg
    11581 ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg caaaaaattg
    11641 ccactatgca aattaattac agggttaata ccgccagatt acgttccacc agcgaattgc
    11701 ccatccccgg cacctgcttt agatcctcca ccgttttaaa cggaccgtac tcttcgcgat
    11761 aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg gctagctctt
    11821 ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct ttcgccggta
    11881 ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc gtcggtttcg
    11941 ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg gacagggtaa
    12001 tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta acagtggaat
    12061 caccttaacg ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg aaaaggccgc
    12121 gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg cttcaggatt
    12181 attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg ttactcatca
    12241 gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc gcttttttct
    12301 gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc acattacctt
    12361 gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc agtgcaaacg
    12421 ccgcctggct aatcgggtca cgaccggaac ggcttaaggt tttcggctcg ccaaatttca
    12481 gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc agcagtttct
    12541 cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc ttaacttgtt
    12601 cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg cgcagcacga
    12661 atgcgcggtc gccgtctacg gtgatgatgt cagagttgat gcccgacgcg ccgttttcac
    12721 ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac tcttccggca
    12781 ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcagct tgctctgcac
    12841 cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc agcgcatagt
    12901 acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt acttcgtcta
    12961 acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa ccgaccgaag
    13021 atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc agttcgtcgg
    13081 ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag ataatatcgg
    13141 cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca tcaagtaccg
    13201 ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc tgcggctggg
    13261 tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta accggttgct
    13321 gcatcgttgc ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc gtcatgaaat
    13381 tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc tgcttcgccg
    13441 ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg accagtgccg
    13501 ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacgccg ttaatcagct
    13561 gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc atccccatct
    13621 ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg aaggctgggg
    13681 tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc tcacgagcgt
    13741 actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc agggttttca
    13801 tatagccttc gttcgctgcc agctcggagt attgatcgcc cagctgttgc tgcatacgat
    13861 tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg tcattcactt
    13921 ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc aatatgaacg
    13981 acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc gtgcgtaagc
    14041 tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac gcaacgtatc
    14101 gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc acaagacaca
    14161 gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg aacttcgtca
    14221 catccccact ggggacaacg cttagtttac cgcgtctttc agtgctttac ctgcacggaa
    14281 gctcggtact ttagcggcag cgatggtgat ctctttaccg gtctgcgggt tgcggccagt
    14341 acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta catcatcccc
    14401 ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc cagccgcagc
    14461 tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat tcactcttct
    14521 cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc gcagcagtta
    14581 tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga tccgctatct
    14641 aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc ctgtttttat
    14701 tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca gacggttcat
    14761 tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg tccagatcgg
    14821 caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga attagcactg
    14881 ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg atcggcagta
    14941 cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc gggttaccgg
    15001 tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca tctttcggcg
    15061 tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca gggttgatcc
    15121 ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac tcctgcatca
    15181 cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat gcggtttcaa
    15241 tggtcagcaa gtcaccgccc acttccgtcc acgccagacc gattacctga ccgacacggt
    15301 tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca tgcaggttat
    15361 cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta accgctttgc
    15421 ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg gtgtagtaac
    15481 gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc agtgcattac
    15541 gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca tcttcggtat
    15601 aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg ttcatggagt
    15661 tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg tagtggtcgc
    15721 tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc ggatcgcctc
    15781 gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcggattt ttcacgccca
    15841 ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta cggcggtgac
    15901 cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat ttacgcccgg
    15961 tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc cctaccaggc
    16021 agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac tcaaggatgc
    16081 gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc gcctgacgca
    16141 ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag tcgatataac
    16201 cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc tgcaactctg
    16261 cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg cgcttcaggg
    16321 cttcgttttc gtccggcacg tcgtccattt caccgagttc tttctgaata gctttcattt
    16381 gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg cggttgcgaa
    16441 tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc agatattcca
    16501 gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt ttcagcggca
    16561 tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc agcgacgtca
    16621 gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg ctgattgcag
    16681 tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga tactccgcct
    16741 tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc tgtaacccct
    16801 cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata gaggccacgg
    16861 tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct ttctgcgcga
    16921 ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata gatttttccc
    16981 gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc agcggcaata
    17041 cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt agtttaattt
    17101 ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat gtacgtcagt
    17161 atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta aaggggagat
    17221 aaaatccccc ctttttggtt aactaattgt atgagaatgg ttaattattc accagatgcc
    17281 tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatc aattaccgac
    17341 tcgtcgataa ccactttttc gacgtcttcc atggacggca gatcgtacat ggtatcgagc
    17401 agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc catcgctttc
    17461 ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc cagattaaac
    17521 agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg aatcagagct
    17581 tcttcgctca gttcattcaa cgttgctaca accggcagac gaccaatgaa ctcagggata
    17641 agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc ttcgcttgct
    17701 ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt ttctacacgg
    17761 tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag gatcttagag
    17821 gtatcaacct gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg tggaacagca
    17881 gctaccgtac cttcgatcag tttcaacagt gcctgctgta ctccttcacc ggaaacgtct
    17941 cgggtaatgg acgggttgtc tgacttacga gaaatcttgt cgatttcatc gatgtagaca
    18001 atcccgcgct gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt ctgaatgatg
    18061 ttttcaacgt cttcgcccac ataaccggct tcggtcagcg tggttgcgtc ggccatggtg
    18121 aacgggacgt ccagcaggcg cgccagcgtt tcagccagca gcgttttacc ggaaccggtc
    18181 ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt atcgccgttg
    18241 cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc ctgttcctgg
    18301 ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag cgcactgcgt
    18361 tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca taaatcaaca
    18421 cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc atgctggctt
    18481 ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt atctgtcatg
    18541 ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc cattctcagg
    18601 cgcaagccgc ttacagcgtt gtgccgccct agataagtat agcggcacag ttgcgcctct
    18661 ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc gcttcagggg
    18721 cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat tgacccgtat
    18781 gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg gcatgaattt
    18841 cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc acgcgcgaat
    18901 tcggcaggca aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg cccatcgagg
    18961 ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg gtgtcataga
    19021 tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag atatcttttt
    19081 ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc atgtggtctt
    19141 caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag atatcaaaag
    19201 agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata tggggtgcaa
    19261 agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg aaaaaaacct
    19321 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
    19381 catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt
    19441 tcgcacggct ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
    19501 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
    19561 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
    19621 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
    19681 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
    19741 cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
    19801 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
    19861 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
    19921 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
    19981 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
    20041 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
    20101 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt
    20161 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
    20221 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
    20281 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
    20341 ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac
    20401 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
    20461 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
    20521 aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
    20581 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
    20641 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
    20701 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
    20761 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
    20821 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
    20881 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
    20941 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
    21001 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
    21061 gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt
    21121 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
    21181 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
    21241 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
    21301 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
    21361 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
    21421 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
    21481 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
    21541 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
    21601 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
    21661 agcgtttctt ttaccagggg tgattatttc gtcaacatga tgaatttaca tgccttaccc
    21721 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
    21781 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
    21841 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
    21901 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    21961 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc
    22021 gctctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    22081 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    22141 tcccagggca acgtgcgcta caacatcgct accaaagcgg atatcgccat catcgctacc
    22201 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    22261 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    22321 gctgatatgt ctcaggacac cagcattcac gaattcatca agcagaacgc gcgttaattt
    22381 ctgccctctg gcccggtgca agccgggcct gtagacgccc atgtccagtc aatatttacg
    22441 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    22501 gctcgccctg acatccggca ccttacaggc ctggatgacg gtcgaaaata tcgatctcaa
    22561 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    22621 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    22681 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaaccag gcacccaact
    22741 ccgctggatg gcggcgctgg cggtggtgat cgctttttgc tctgcctccc aggatattgt
    22801 cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat
    22861 cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcctagccc tgtggctggc
    22921 agataaatgg ttgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccctg
    22981 tattatcgcg acgttgcttg caccagaacc aaccgacacc attcctgtgc ccaaaacgct
    23041 ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat
    23101 tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac
    23161 gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtaa acaaaacgct
    23221 tggcttactg gcgaccattg ttggcgcatt gtacggtggg attttgatgc agcgcctgtc
    23281 gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg
    23341 gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa
    23401 cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc tgtgtaataa
    23461 gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag ggcgagttta
    23521 tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt
    23581 ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga
    23641 atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccggcag gttatgcctt
    23701 tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac ttctgttgac
    23761 gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt
    23821 tttagtcgcc ctttctggcg tcatgcttgg tggtttgctg gattatctgg cgctacgaaa
    23881 aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat
    23941 tacggctaaa taaacatcag catcgcttat taatatttgt tcttttgtgc ggcttagcgt
    24001 ttggaaatta ttgacgccat ttataaattc tatttttctt acacgattca gctaatgagt
    24061 ctttattttc tcaccaccca gttgtcactt taatgataat tatttgttaa ataattgttt
    24121 tatttcgcat tggttatacc aattgcccgc caagactcct tctgcactcc ccttttgtta
//
LOCUS       DBKELA010000060        23532 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.060, whole genome shotgun sequence.
ACCESSION   DBKELA010000060 DBKELA010000000
VERSION     DBKELA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.060"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..366)
                     /locus_tag="ACUQR0_002377"
                     /pseudo
     CDS             complement(1..366)
                     /locus_tag="ACUQR0_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gp53-like domain-containing protein"
     gene            complement(727..1116)
                     /locus_tag="ACUQR0_002378"
                     /pseudo
     CDS             complement(727..1116)
                     /locus_tag="ACUQR0_002378"
                     /inference="COORDINATES: protein motif:HMM:NF023985.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(1113..1724)
                     /locus_tag="ACUQR0_002379"
     CDS             complement(1113..1724)
                     /locus_tag="ACUQR0_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HHV9109380.1"
                     /translation="MSDSRLLPTGSSLLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(1717..2625)
                     /locus_tag="ACUQR0_002380"
     CDS             complement(1717..2625)
                     /locus_tag="ACUQR0_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HHV9109381.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYSVVTGGSDE"
     gene            complement(2630..2977)
                     /locus_tag="ACUQR0_002381"
     CDS             complement(2630..2977)
                     /locus_tag="ACUQR0_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HHV9109382.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(2974..3609)
                     /locus_tag="ACUQR0_002382"
     CDS             complement(2974..3609)
                     /locus_tag="ACUQR0_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098025 - virus tail, baseplate [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HHV9109383.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSEGEQVLILAVGGELDTAFVLPGIYSGDNPPPSASADALHI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3676..4128)
                     /locus_tag="ACUQR0_002383"
     CDS             complement(3676..4128)
                     /locus_tag="ACUQR0_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HHV9109384.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(4121..4588)
                     /locus_tag="ACUQR0_002384"
     CDS             complement(4121..4588)
                     /locus_tag="ACUQR0_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9109385.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4551..4796)
                     /gene="lysC"
                     /locus_tag="ACUQR0_002385"
     CDS             complement(4551..4796)
                     /gene="lysC"
                     /locus_tag="ACUQR0_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640305.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HHV9109386.1"
                     /translation="MLCAGCTSARPAPTPVIVANACPKVSLCPMPGSDPETNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(4696..5121)
                     /gene="lysB"
                     /locus_tag="ACUQR0_002386"
     CDS             complement(4696..5121)
                     /gene="lysB"
                     /locus_tag="ACUQR0_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HHV9109387.1"
                     /translation="MSKLMIVMVVLLSLAVAGLFLAKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGAGLPDAVRRLHQRPACTDASDCRQRLPESEPLPDAGQ"
     gene            complement(5109..5534)
                     /locus_tag="ACUQR0_002387"
     CDS             complement(5109..5534)
                     /locus_tag="ACUQR0_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HHV9109388.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQS"
     gene            complement(5549..6046)
                     /locus_tag="ACUQR0_002388"
     CDS             complement(5549..6046)
                     /locus_tag="ACUQR0_002388"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 24 protein"
                     /protein_id="HHV9109389.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(6046..6327)
                     /locus_tag="ACUQR0_002389"
     CDS             complement(6046..6327)
                     /locus_tag="ACUQR0_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HHV9109390.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGDEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(6331..6534)
                     /locus_tag="ACUQR0_002390"
     CDS             complement(6331..6534)
                     /locus_tag="ACUQR0_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HHV9109391.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6534..7043)
                     /locus_tag="ACUQR0_002391"
     CDS             complement(6534..7043)
                     /locus_tag="ACUQR0_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HHV9109392.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(7143..7886)
                     /locus_tag="ACUQR0_002392"
     CDS             complement(7143..7886)
                     /locus_tag="ACUQR0_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HHV9109393.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(7890..8963)
                     /locus_tag="ACUQR0_002393"
     CDS             complement(7890..8963)
                     /locus_tag="ACUQR0_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HHV9109394.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVRRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(9022..9876)
                     /locus_tag="ACUQR0_002394"
     CDS             complement(9022..9876)
                     /locus_tag="ACUQR0_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HHV9109395.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRIAEMETAFSALKQDMTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            10050..11822
                     /locus_tag="ACUQR0_002395"
     CDS             10050..11822
                     /locus_tag="ACUQR0_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HHV9109396.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11822..12856
                     /locus_tag="ACUQR0_002396"
     CDS             11822..12856
                     /locus_tag="ACUQR0_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHV9109397.1"
                     /translation="MSKKKGKTPQPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFTPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(13939..16215)
                     /locus_tag="ACUQR0_002397"
     CDS             complement(13939..16215)
                     /locus_tag="ACUQR0_002397"
                     /EC_number="3.1.21.-"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HHV9109398.1"
                     /translation="MAVKASGRFVPPSAFTAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCHIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNQGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNEPEVVMALRGALKHGLRTPNRQ
                     QRNGIPLKPHEIAPSARLTRSERLQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSLPI"
     gene            complement(16205..16480)
                     /locus_tag="ACUQR0_002398"
     CDS             complement(16205..16480)
                     /locus_tag="ACUQR0_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HHV9109399.1"
                     /translation="MSIRIEIGKRYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLGQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(16477..16701)
                     /locus_tag="ACUQR0_002399"
     CDS             complement(16477..16701)
                     /locus_tag="ACUQR0_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HHV9109400.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(16701..17003)
                     /locus_tag="ACUQR0_002400"
     CDS             complement(16701..17003)
                     /locus_tag="ACUQR0_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HHV9109401.1"
                     /translation="MSCSRSVVLLNNALKIAVMENGDLSLIQLGLDKEKRDITESVIA
                     IYQSELNLLSDVINLLVKRAVFHRQISSVDELTKLTTELASYCADEFKKLNDKRNW"
     gene            complement(17003..17227)
                     /locus_tag="ACUQR0_002401"
     CDS             complement(17003..17227)
                     /locus_tag="ACUQR0_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HHV9109402.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(17291..17791)
                     /locus_tag="ACUQR0_002402"
     CDS             complement(17291..17791)
                     /locus_tag="ACUQR0_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HHV9109403.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(17961..18233)
                     /locus_tag="ACUQR0_002403"
     CDS             complement(17961..18233)
                     /locus_tag="ACUQR0_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HHV9109404.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            18370..18663
                     /locus_tag="ACUQR0_002404"
     CDS             18370..18663
                     /locus_tag="ACUQR0_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9109405.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            18733..19713
                     /locus_tag="ACUQR0_002405"
     CDS             18733..19713
                     /locus_tag="ACUQR0_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase"
                     /protein_id="HHV9109406.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEATP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRIVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(19900..20400)
                     /gene="cpxP"
                     /locus_tag="ACUQR0_002406"
     CDS             complement(19900..20400)
                     /gene="cpxP"
                     /locus_tag="ACUQR0_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HHV9109407.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            20550..21248
                     /gene="cpxR"
                     /locus_tag="ACUQR0_002407"
     CDS             20550..21248
                     /gene="cpxR"
                     /locus_tag="ACUQR0_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HHV9109408.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            21245..22618
                     /gene="cpxA"
                     /locus_tag="ACUQR0_002408"
     CDS             21245..22618
                     /gene="cpxA"
                     /locus_tag="ACUQR0_002408"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HHV9109409.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(22724..23398)
                     /gene="yiiM"
                     /locus_tag="ACUQR0_002409"
     CDS             complement(22724..23398)
                     /gene="yiiM"
                     /locus_tag="ACUQR0_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HHV9109410.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT         5460 a         6480 c         6049 g         5543 t
ORIGIN      
        1 aaatccagtg ttatggacat ttgtcgccga tacagtcggc tgccctgacg cgccagtaac
       61 gttcaatgta actgtgacag caaggactct tgagaatgtc atcgggaaca tcatcccgta
      121 tgttgaagat acattcgggg agccattcaa ctgaccgcac tgtaaaatta atccagacgg
      181 aaatttttgc gcagcagccg ttgcggtatt acttgacgcg aacaggctca tatcaggtat
      241 ctgattcgcc cctgtaccta cattcctttt agctgcttct cccaaaccaa ggtatgcaag
      301 aagaccggca acatccttcc cactcaaatg agtcagcgtc tcatccagtg gctgcttacc
      361 tgacagcgca ttgttaatgg tggtgctgaa tttcgggtca ttgttaatgg ctgcggcaat
      421 ttctttcagt gtgtccagcg tggctggcgc accgttaatc agagcggtaa tagcggcctg
      481 aacaaacgca gtagtcgcaa tccgcgtggt gttatttcct gcggcaggcg tcggcgcttt
      541 tggttctccg gtaaatgtcg gattatgttt ctgtgcatac tgggtatgag gatcctgtgc
      601 ggcaatgtgg tttctcatct ggtcatccac atacagcctt aattccagga cttcatcatc
      661 cacgtattta cgggtcgcca gtaccaccga cgggtcgatt ttcagcgtga tggcttcggt
      721 attcgagaca accagaatca tgcggatagt ctgggtacga ccgcttcctt cctgcaactg
      781 cggtttgtac gtttccgggc agtttgccac cgcaatgagt acgccttcat catcataaag
      841 cccaatctca cggatccaga atccgccctc gttctcaggg atgatttgct ccgcaataat
      901 ctggctctgg ttattcgggt caacactcag aagattcagc ggtgcaatgc gtttctggtt
      961 aatcagtttt gtctgtgccg ggtctggtgt cggcaagaca ccattcgcat caccaacggc
     1021 catttgcgtc agattcagct tactgccgag catcgtcgcg ttagccagcc gtgctgcgcc
     1081 ctgattagtc agaatggcgt agtatttcac tgtcatgcgt ttactctcag gttatcaatt
     1141 aaatgaatgg ccgaggccgg gaaataatcc cctccgacaa taatggcctc cggggtgtag
     1201 ggataaaccg tcagggcgtc gccgtgatag catcccgcac cggcaaaaat gttgccggtt
     1261 gtacttaaac tgatagccag tcccgtcaga tggcggcttg caggttttgc atcagcaacg
     1321 agacgctcca gctcctgata catttcctcg gtaataccct gctcaagcac gccaacaacg
     1381 atgcggaacg tccccggctc ctcgttgagc tgccaccact ccctcacctc aatcagatag
     1441 ccgagcggct ccaccacacg gcgaatcgca ccaatagtgc ccttatggca gtgaatgaaa
     1501 tacgcatcgc ggataacagc gcgtttggtc gcttccggcc atttatcatc ccagcggtca
     1561 accgaaaatg accacgccag ccacggcagc agatttgccg ggcaggtatc cgggttccac
     1621 agctcacgaa tactgaccgg cgttttttca atttctgcac aggcttttgc ggcggcaact
     1681 tcaagcagtg atgagccggt cggcagcagg cgcgaatcac tcatccgagc ctccggtcac
     1741 gacgctgtat tcggtgcaga aagacgcctg cgtactgttg agcacgatgt cagccagcgg
     1801 tgcagtcagt tcgacacgct gcacgccttc cacatgcaaa gcggcataaa tggcagacag
     1861 acggatgtcg cgcccaagcc ggtgctgtgc cgtgatgtac gcttccagtt ttttcacggc
     1921 agcagcgcgg atgggttcgc tttcaggacc tgggtaaagg taaagcgtgg cgtttatctg
     1981 atattcaaca atggcggcag actgcacggt cacgcggtcg gccaccggcc tgacgtcctc
     2041 gccattaagg gcgttacgca ccacggccag caggtcttcg gatgcgacgc cgttattttc
     2101 acgtgacagc acggagatag tgacgcaggc cggagacgga ctggtgacag agatatccgc
     2161 gacacgcccg tcagcactgc gaccatgata ctgataggct cccaccgacc cggcgacgct
     2221 taagccctca aacgcctgct gaatacgcag acgataatcg gtgtcagact ccatcactgc
     2281 cggtgtcggc ggaatagtcg aatcatctgc cggggtgata atcaggcgcg tggtgttgta
     2341 attggcacca atcacatcaa ggtcattacc ggcggcacag gccagcatca ccgcccgtgc
     2401 ggcctcattc acacgctgac gccagataag ctcacgataa gcattttcct ccagcagttt
     2461 gacgagaggc tcggattcca gcgtcagggt acgggcgacc gcctcctgct ggtcttccgg
     2521 gtaaagggaa atcagtgtcg ccttgcgttc ggcaagaatg gtttcaaagt ccagctcctc
     2581 gaccacatcc ggtgcgggta gctggttcag gtcgataatc ggcatggttt caactcacag
     2641 ggatggttaa cgaaagtggc tggccggtgt cgttgtgctg gccggttaac gtgaccgtca
     2701 ttcgcccgtc aaaactgcgc tcagtggtga cggatgacag ggtgacgcgg ggttcccatt
     2761 tcagcaccgc catgtaacag gcgaccttaa tctgcaactc aagcgccggg gtctgcggct
     2821 ggtcaatcat tgatgccagc aacgagccgt aatcacgacg catcacccgt gagccgaccg
     2881 gtgtacgcag gatatcgccg atactctggc tgatatgctc aaggtcagtg acagtcaggc
     2941 catcactgcg attcattccg agataacgcg ctgtcataaa ggactcccgg ttgtgccgcc
     3001 gctgtcgccg gggtgtttat gggtatgcag taccttaccg tttgatgaga gttcaccgcc
     3061 ggtgtgttca atgttgccgc gcatcgtccc gcccttctgt acttccagcg tgccggtaat
     3121 cagcctgttg gtgcagacca cctccggtgt gtccagggtg atgcgggttg acgctttcac
     3181 catgaccacc ggcaccgtgg cagtaacaga atcagaagcc gtcacgctgg ccgttttaat
     3241 tccgcttgcc gtgagcgcac tggtttcagg ttcatactca atcaccgccc cgtcagggaa
     3301 acggatatgc agggcatccg ccgacgcaga cggcggaggg ttatcgccgg aataaatccc
     3361 cggcagaaca aacgctgtgt cgagttcacc gcccacggcc agaatcagca cctgttcccc
     3421 ctcggaaggt gcccaccacg tgcgcgaacg tccggcgcga tgggtcagcc actgaagcca
     3481 gtcggtgcac atgccgccgg tctgcacacg gcagcgaccg gcgttaaggt cggtttcgac
     3541 gataatgccg gtgcggatca tgttgcgcag tgcgcgcgcg agttcctgaa tatttgcgag
     3601 agtgttcata acgggaagga tgccgccggg tcataccggc ggcaatgtga cgatgaggtg
     3661 tcgggaatgg cacaactaac ggtcgaggtg agccaggata atctcttcaa tcatctgcac
     3721 atcctcaccg gtaaagccga gcagaggacg cgccggataa tcaattttct taccgtcttt
     3781 ccggttttct tccgacagac cgaactgatg cacgctggcg attttcggtg acttcccgcc
     3841 gtaaaactcc attgatgcct gttcagggct ggcgcggata tgcaaaaaac gactggtaat
     3901 aagtttcgca aacatttttc gcttaacacg accagtcttt tttctggcgc tctgctgctg
     3961 gcgtggcgcg tagggtgtgc cgtccggggc tttctgtgcc atcacccgac gctgctgact
     4021 ctgtcgcaga cgttttgcca gttcggcact cagtcgccga cgtcctgacg gtgacagcga
     4081 ctcaatcagt ccggtcagcc ggtcttcaaa acgcttaaac tcattcatcc cacttactca
     4141 ccagttcacc attgatataa agctccatcg ggcgggtgac cggctccggc ggcgggggtt
     4201 ccgggatatt cttcacatgc agtgcgccgt ccacctcact gaccagcgtg cgctcggtca
     4261 gcatcaggct gatgctgata tcaaagctgc tgtcattgtt gatgtctgca taaaacgtga
     4321 agcccttttt ctggcctgcg tcggtggtca tgatgtcggg ctgattttcc cgcagccacg
     4381 ccagcaccgg cacgatgagc aggtcaaaat caccggtaaa gtcggtcaca atcacattga
     4441 gcgtgtaacg cttttcgaat gacaacgacg tcgccagcgt ggaggcaata ctcccgttat
     4501 caacgaatat ccgcagcatc tcgggactgg ttttcagcac cgtgacggca tcagtcagcg
     4561 ccctgcgcag gctgtcgggt ttgagcatcg ttttcgtcct gacagtgttt aatcattttt
     4621 acctggctgg cacagcgtgc cagcgcgttc tcaagctgtc ggatatcggc acttaaatcg
     4681 ccgttcgtct ccgggtcact gcccggcatc gggcaaaggc tcactttcgg gcaggcgttg
     4741 gcgacaatca ctggcgtcgg tgcaggccgg gcgctggtgc aaccggcgca cagcatcagg
     4801 caggccagcg ccgtaccagc ggcgaaaatc ttcgttttca ttcagtaacc tcgtgatggt
     4861 tttctcgcgc tgtgcttcac gcttcgccgc gttctccagt tcctgacgca gtgccacctg
     4921 cgccagctcg tttttgtctg ccctggtgat ggcaacatga agctgatttt tcagcatggt
     4981 gatggtcgtc tgctgttcac tggcgacgtt attcgccctg tccagcgagg cgcgcaggct
     5041 ggcgttttta tgcttcgcca gaaacagacc cgccaccgcc agcgataaca acacaaccat
     5101 cacaatcatc agctttgaca tggttcccgc ccctcaaaac gctgacggca ggccgtacgt
     5161 atcagccgga agaacaccga tgccacgaga taaatcagcg cggtaaaaat ccacccggca
     5221 gcgaccagcg agataaacgt cgccaccatc accaccagag ccgccgcccg cctgcgccac
     5281 ggcaccggct gtaaaaacag cgacgtgaca atcttcacgg ccagcgattc cggcggcagc
     5341 tcccgtccgt agcgttccag tacatactcc gtggcataca cgccgacacc accggcaacc
     5401 acacagataa ccgtcgccag aatcgcccag gcagcgacaa aactgacggc cacgctctgc
     5461 gggtaaatca gggacagtgc cagcatcagc gccagcgaca cgtgcagcat cagtgaaagg
     5521 gataatttct tcatggtgtt tactccgttt aagccggtac gccgccagcg gtacgccaga
     5581 cggtgaccag tttttccagt gaatgctcac gctgaccgta accggcaccc ggcagggacg
     5641 cccagatatt gcgacagcgt gaaatggcgc gctcaatgcg tcccgcccgg atgtcatcca
     5701 gtgctccgcg ttcgcggatc aactgaatgg cgagtctgtc ctgtgacaac ggactgaaat
     5761 ccggcagggc aagctgtttg cggtagtgcg gccagaacag gtaaagctgc tgatagcgac
     5821 cggatgccgt ggacttttca ccgcgacggt taaacacctt cgccggtcgg ccatgcgcga
     5881 acgggtggtc actgtagtcg gtgaaaattt ccggcttccc gtccagtccg gtgactatca
     5941 cgtcatagcc ccggtttttc gtcagcggat gattcgccgt cccttcggac acggccagca
     6001 tgtcgagaaa ggcggcgata ttctgatgtg tgttaattac cggcattacg gtttccccct
     6061 gcccttaaag cggcgctgaa tggcaatctc aatcacctga taaccggcga tacccagcat
     6121 ggagccgatg ccgcacaccg caggcagtga caggtcagga aactgcacca gaacaacacc
     6181 ggcaaccatc gagacaaaac caccgagcaa catgcgcccg ataaacagac gcggggtgat
     6241 gggttcatca ccggcaagca ccttgccgac aacaatcagc accccaatca tgaaaagcga
     6301 caggacgctt ttttcttctg ctgtcatgcg ttactcccac agattgacag tttcagccac
     6361 gggcgcggtc tgaacgtcgg gcagttcgac ggcggtgccg tgtggcagca ccgcacccag
     6421 ttcagccagt cccggatttg cggcgagcac ggtctcaacc acgccctcag tgcgcccgta
     6481 ataccggaca caaatggcgt cgagcgtgtc gccctgtagc gcaaaggtct tcatcagatt
     6541 tgactcacga tgcagcgcgg cttgtcctgg atgcgcgcca ccgcccagcg catatcccgc
     6601 cacagctcat caatggtgct gtcaatgctg tcggccttct tgtcgccttt cgcactggca
     6661 tccacgccgc gataacgctc ataaagcgat gcggtcgcca tcgcacacac ggcgcgctcg
     6721 tagtaaaaaa ctttgatgct ttcaccgtcg atgtcgtccg ccgggacgtc cgccagacgc
     6781 gtaaaaccgg cggcaatttt ctgttcgcgg tactcgtaca gctccgcatt cgtctccgcc
     6841 atgcctgact tgatggcctc acgcagacgg gcgggggcga cagtctgctc aaggcgcata
     6901 cgttcccgga cgcgcttcgg gtcgatatcg ggaaaaaaga acgtattttt aatcaccggc
     6961 tcgtcgcctg ccggttgcgg gatgaccacc gtaccctcac cggacacggg agcctccttt
     7021 cgcggaataa tcagcgtcat catgactacc tctgaaaagt cgggcggtgg acgccggtgc
     7081 agtgtcaggt gattcaccct cactgaccgg cgtgccgccc tggcgcgggg cgcattcggt
     7141 tgttaactgg ctttcttttt cgggcgtcca cgttttgccg gtgtcacgct ccgggtctta
     7201 cgcggggtac gggtggccgc ttttggctgc ggctccggct tcggtttcag ctcccgctcc
     7261 agtcgttcaa tctctttttt gacgcctgcc tgacagtcga gctgtgtcgc acgttgcagg
     7321 tgagccagcg caccggcggc atcaccagcg tcacgcagaa acagaccggt gattttgtgc
     7381 agctttgcgc gcacttcatc aggcatgtca gccgtggcgg tcagttcaag ggtgtccgtc
     7441 agcaggcggg tatccacaga ctcaccggca gcgtgggcac gcatggccgc aagcgcgacc
     7501 tcctcggtga acatgtacgg cggggtgcgg cggtgttttc ccggcatggt cagaccgtac
     7561 ttcagggcat aacgggcaat ctccagcgca ccggcaatat cgccggtatc cagacgccac
     7621 agcatgaccg tcatcagaat gtcatcctgt gcacctttgc cctgctccag cacgccgttc
     7681 acccacggca accagaacgg cagcagttcg cgctttttcg cggccttcag ctcttttgaa
     7741 taaatcgctt tcagtgtgcg ctggtctgcg gcgagcttaa ccagcatctg ctcatagaca
     7801 gttgcatgtc gcagcggggc ggcgtcccgc tgcgcggtca tcgctgccga gacccgcatc
     7861 atgtggcgct gtgcgggact cgtcatcagt tacgctcccg gctctgcggt cgccttagcc
     7921 ggtgtggaga agtcaccgac cttgattttt tccaccagac aaccggcggc gtagtcttcc
     7981 accacgtaat caatgttcat tgactcgtag ttctccacgc ggtcgagttt cgggttttcc
     8041 acaatcacgc ggcgatggct gtcatccatg tagtagatgg acaggttttc cagcttcgtg
     8101 atgagcatcg catccgccgg gaagtacggg acgcgtaccg ccggcaggtt accgatgcgt
     8161 ttctggctga tgatgacgtc agcggccagc atttcgctgt tgtcctgctc cttgttaacg
     8221 atggggaaat acttgtccgc cagtaactga cgccccacaa tcaccacaag gtcagggtct
     8281 tcctgatacc acggctcaat caggttgttg gtcgcatcca tcaccagtgc atcaaggctg
     8341 gcataatcac cgcccttacc cacgcggatg acctcagagg tggtgtgacc ttcctcgtca
     8401 gtgaccttgc tcatcacgcg cgccggggct tcattgcggt atttctgcag ccagccgacc
     8461 gccacatcct gcagcatcgg attgctgctg cggtcagagg tttcggcacg cctcacgccg
     8521 ttaaaaccgg ccatgatgaa atcaagggac tggcgtttga taatggcgtt acggacacgg
     8581 agctggaaat cctgataacg cgcccacagg tccagcgttt tgtagcggat ataaaaatcg
     8641 aagttaatct ggtcgcattc gtacttgttt gacgccagct tcgagaagtc cttcggctga
     8701 cgctcggtgc caccggcggt gtcggtggtg ctggcgatgg agccggtgac accgatacca
     8761 attttttccc ctttcatttc gctgaccggc acaatgttga tgcgggtcag aaaatcagag
     8821 gactcctgca tggtgttcat cagggtctgg gtgaccgacg gttcaacggt gaattttttc
     8881 gacacatcac cggcgtcgat gccgttcagt tcggcaacac gggacaggta ggcattaaat
     8941 ttaaagcggg tttcctggcg catagttttt cctgaaatta agggttaatc gtgaaggttt
     9001 tcccggactg actgacgccg gtcagcagtt cgtcatcagg gcgtcaccgc caccgccggt
     9061 ggccttgctg cggcgctgct gggtcagact ttcggtgtgg tcgagactgt ttttcaggcg
     9121 ggtgaatgcc tggctggttt catccgccct gtcagtcata tcctgcttaa gtgcggaaaa
     9181 ggcggtttcc atttctgcga tgcgctgctc agtggcgctc agtttttcct gcacatgttc
     9241 agcaacagcg gtcaccgctt catgcacgtc attcagacgg gcgtcatcgc tggcctgttt
     9301 acggccaaaa atggatttca ccttttcggt cagggcggtg aacacggttt caggcaggtc
     9361 ttcaaattcc agctcaacgg gcgttgccac tgaaatcagg ttttcagggc ttaatttgaa
     9421 gcggttcagg gggttgtgtt ttgccgtgcg gcagaattcc aggtattccg tgccgaggct
     9481 tgccgggtca tcggtgacgg ccagacccac cagataacat ttgccggtat tggcaaagtt
     9541 cggctgaatt tccattgagg tatagacctt ctgcgcggcc ttgttcatcg cgataaggtc
     9601 atcggttggg gtgattttcg caaacagcgc ccatttgcct ttcagcgccg aatcatcgtc
     9661 aatcttttcg gccttcagtt cggccacatc gccataacgt ttaaaaatac cgtcaggcag
     9721 gatgccgcgc agatgttcca ggttaatgcg gcaaccatag actcgcgggt caaaggtttc
     9781 ggccatttcc tgaatatcct gcgcactgat gacacgtccg tcacaggtgt caccctcaac
     9841 gccgatacga aagaattttg agactttttt tgccattgtc aggagtcctg aatagtgatt
     9901 agaggagtca catgtcggca tcagtttccc gacgatacgc atcctccgcc atcagtcccg
     9961 gatggcttat cactgacaca acagcacctt agcgaatcgc ggggcgcgac tcagtagcct
    10021 tgccgtgtat tcatcacggc gaggtattca tgaccatcac cacagacacc actcttttac
    10081 acgacccgcg tcgtcaggcg gcgctgctgt actggcaggg gttttccgtg ccgcagattg
    10141 ccgccatgtt gcagatgaaa cgtccgacgg tgcagagctg gaaacagcgc gacggctggg
    10201 acagcgttgc ccccatcagc cgtgtcgaaa tgagtctgga agcgcgactg acccagctca
    10261 tcatcaaacc gcagaaaacc ggtggtgact tcaaggaaat tgacctgctg ggacgccaga
    10321 ttgaacgact ggcacgggta aaccgttaca gtcagaccgg caacgaggca gaccttaatc
    10381 cgaacgtcgc taaccgcaac aaaggcgggc ggcgcaaacc gaaaaagaat tttttcagtg
    10441 acgaggccat cgaaaagctg gagcagattt tctttgagca gtctttcgaa tatcagctgc
    10501 actggtatcg cgccgggctt gagcaccgca tccgcgatat cctgaaatcc cgccagattg
    10561 gcgcgacgtt ttatttttcc cgcgaggcgc tgctgcgcgc cctgaaaacc ggccataacc
    10621 agatttttct gtcggccagt aaaacgcagg cgtatgtgtt ccgcgaatac atcatcgcct
    10681 ttgcccggct ggttgacgtt gacctgaccg gtgacccgat tgtcctgggc aataacggcg
    10741 caaaactgat ttttctcggc accaactcca acaccgcaca gagccataac ggtgacctgt
    10801 acgtcgacga gattttctgg atcccgaatt ttcaggtact gcgtaaggtg gcatcaggta
    10861 tggcctcaca gagtcacctg cgctcgacct atttctccac cccgtccacg ctggcgcacg
    10921 acgcctaccc gttctggtcg ggtgaactgt ttaaccgggg acgcgccagc gccgccgaac
    10981 gcgtggaaat cgacgtcagt cataacgccc ttgccggtgg gcttctctgt gcggacggcc
    11041 agtggcgaca gattgtcacc attgaggacg ccctgaaagg cggctgcacg ctgttcgaca
    11101 ttgagcagct caaacgcgaa aacagcgccg acgattttaa aaacctgttc atgtgtgaat
    11161 ttgttgacga caaggcatcg gtattcccgt tcgaggagct gcaacgctgc atggtcgaca
    11221 cgctggaaga atgggaagac tatgcgccgt ttgccgccaa tccgttcggc tcccgcccgg
    11281 tatggattgg ttacgacccg tcacaccgtg gcgacagcgc cggatgcgtg gtgctggcac
    11341 cgccggtggt ggccggtggc aaattcagaa tacttgagcg tcaccagtgg aaaggcatgg
    11401 actttgccac tcaggctgaa tccatccgca aactcactga aaaatacaac gtcgaataca
    11461 tcggtattga tgccaccggc ctcggtgtcg gcgtgttcca gctcgtgcgc tcgttctatc
    11521 ccgccgcgcg tgatatccgc tacacgccgg aaatgaaaac cgcaatggtg ctcaaggcaa
    11581 aagacgttat ccgccgtggc tgtctggaat atgacgtcag cgccaccgac atcaccagct
    11641 cgtttatggc tatccgcaag accatgacca gcagcggacg cagcgccacc tatgaggcca
    11701 gccgcagcga ggaagccagc cacgccgacc tcgcctgggc gaccatgcat gccctgttaa
    11761 atgagccact caccgccggt atcagcaccc cgctgacatc caccattctg gagttttact
    11821 gatgagcaag aaaaaaggga aaacaccgca acctgcggca aaaacaatca ccgccagcgc
    11881 cccgaaaatg gaagcattca cctttggtga accggtgccg gtactcgacc gccgtgacat
    11941 tctggattac gtcgagtgca tcagtaacgg cagatggtat gagccaccgg tcagctttac
    12001 cggtctggca aaaagcctgc gtgctgccgt acatcacagc tcaccgattt acgtcaaacg
    12061 taatattctg gcttcaacgt ttattccgca cccgtggctt tcgcaacagg atttcagccg
    12121 ctttgtgctg gattttctgg tgttcggtaa tgcgtttctg gaaaagcgtt acagcaccac
    12181 cggtaaggtc atcagactgg aaacctcacc ggcaaaatat acccgccgtg gcgtggagga
    12241 ggatgtttac tggtgggtgc cgtccttcaa cgaaccgaca gccttcacgc ccggttccgt
    12301 gtttcacctg ctggagccgg atattaatca ggagctgtac ggcctgccgg aatatctcag
    12361 cgcccttaac tctgcctggc tgaatgagtc ggccacactg ttccgccgca agtattacga
    12421 aaacggcgcg catgccggat acatcatgta cgtcaccgat gccgtgcagg atcgcaacga
    12481 tatcgaaatg cttcgcgaaa acatggtcaa gtcgaaaggc cgcaacaact ttaaaaacct
    12541 gtttctctat gccccgcagg ggaaagctga cggcattaaa attatcccgc tcagtgaagt
    12601 ggcgacgaag gacgattttt ttaatatcaa aaaagccagt gccgcagacc tgctggacgc
    12661 gcaccgcatc ccctttcagt tgatgggcgg caagccggag aacgtcgggt cgctgggtga
    12721 tattgagaaa gtggcaaagg tctttgtccg caatgagctt atcccgttac aggacaggat
    12781 tcgggaaata aacggctggc tcggtcagga ggtcatccgc tttaaaaact actcactgga
    12841 cactgacaac ggctgaacat cgccgcctgc gggcggcttt tttacacccc gtcatcacgc
    12901 cctcacacgc tcaccaccgc gcaaaacagc ccgcagacaa accaacgccc cggcgcacaa
    12961 tctaaacgcc attacgacgc gctgagacgc tgaaaaaata aaatcagcac caccgccagc
    13021 gcgcagtgct ttccccgcct cgcccgcccg cttcacgggg cggttttaat gcagttgcat
    13081 agatactatg gatccgcacc agtcctgacc gcacgcagcc tgaacggaca tccccaacgc
    13141 atgcaaaaac attcacttgt tgcatgcata gctttttaag tacgccatac cgcaactgta
    13201 catttttaag caattggcaa ccttaaaaaa tttacattgc tttcaagacc ttatcatccg
    13261 tagtctctgt tttttacttt gagctacatc aataaaatct caaacatgtt taatgcaaag
    13321 cccttgttac acaacataga atgtatgtct agaaacaacg acatactata tgttgtgttt
    13381 ttccggctct ctgttcggtg atatgccaaa tcactttgcg tttaaacaga gcattatttc
    13441 agggcaagac ttcgctcaaa agtcattcca cctaagaagc gcatataccg gtggaagttg
    13501 ccctctactt acaggaggca atatgaagaa atgctattac tgcattctcg ttctggccct
    13561 ctttggctat ccaaacggta gtccgagtgg tttgtcagta aacgtcagta atatcaatgt
    13621 cagcattatg ctttaatatg cttcaaacaa aaaaaccacc tgccagggtg gtttttttgc
    13681 gcccatcatc aatacgatga aagactgaca agacttcgct caagacgaat tatgcgtcga
    13741 cataaggtta cccgcaatga ttttattaga ctaaatggat ggccttttat gacccggatc
    13801 aaacggctgt ccttaccgac atgctagtca ctcaatagca tttagccttc taattcagca
    13861 tgctattgac cttctgctgc cccataactg ctccgcataa gccattcaat gccatatcaa
    13921 atcacgttgt gtttttactc aaatgggtaa cgagaacccc ggccactcat cagcaaccgg
    13981 atacgtgaat tttttcccgt cataatttac ggtcgcgcca cgcgccagcg cctcaagctc
    14041 ccatcgttgc ggcctgatac cgttctgagc gaggtcaacg cggatacggg taatttgcaa
    14101 tcgttcagac ctggtcagtc tggcagatgg tgcaatttca tgcggtttta acgggattcc
    14161 gtttctttgc tgacgatttg gtgttctcag tccgtgtttt aatgcgcccc tgagcgccat
    14221 cacgacctcc ggttcattcc attcaataac accgtcatca accagattaa gcactgctgc
    14281 ggcgtgctca gaaggtgtgg gagccggtaa cgaagtatca ccaccggtga gctttccaca
    14341 gttattgaca ggactccgag gcgcggcgat gccgcttttt aaagtcagag gctcaacgac
    14401 cggaactttc ggcacaatgc gccagtccgt cgttctggtg atatgaatat gacgcgcgcc
    14461 gagatgcggc gcgtaaatgc cgaccactct ctcgacttct tcctcgtact cgttaacgtc
    14521 atccgacgga ctacgggcga ccctgacagt ctgacaatcg cgcgggacat ttgccccacc
    14581 ctgcgcgctg atatacaacg caaaatcacc actgtctgcg gcggcgcgtg cagcctcgac
    14641 gcgctcgtca aactcatcag caatgctgac gccgcgaggc aatttgcgta gttcacggta
    14701 agcccccatt gtcggcagac caaccgtttt aaattgtggg atgcgccacg ttgacgccca
    14761 tgcggtaaca gccgcagcag tgtctttcag cggtctgccg gtatcgttat cgagctgacc
    14821 atccagtgca tagccatcga tgttttttga gatgtatttc gcgatatacc ccgcagcacc
    14881 gccctggtta aggtgttttg cctgaaaacg gtttcgcgcg gctcctcttt cgtcgccatc
    14941 ctctttgagc gcatagcgac gcatgatttc gataatctgg ttacgctggc gtggattaca
    15001 aaaaagcatc atatgccagt gcggcgttcc gtcgtggtgt ggctcgacga cacgcaaacc
    15061 gtagacctgt aaatcattat ccttgaatgc cgtgcgcatc aggctccaga tatggcagag
    15121 ataacgctgc gcatcctttg gattaaatgc ctcatcgttc cagccgtgat ttagctggac
    15181 ggttttactt tcgccttttc cgacctgacg tgtcgggtga tactttgacg gcgcggtcag
    15241 cgtgataaac atccccacat caccctctgc ggcggcgtaa cgctcaatac cggcaatggt
    15301 gttcatcagc tccatccggc gaatttcagg attagaaata ctgcccatca ccttactgat
    15361 aaggtcgatg cgctcgccgg tttccctgtt ttcaaggtca cacgatttaa gaaattccag
    15421 atttgcctgg cggcgcgcac gcacatcacg aatggcgtgt ttactggcat aaggagaacg
    15481 gtctttattg acctccccga cagctatcag taacgcctca tgccagcgca tacgctggcc
    15541 tttaagctga tgagtccacc actcatcgtt aaacaggcgg gcaatggcag aatatgcctg
    15601 cctcgtggtc atctgtcctt tacggtattt tttccagtag agcggggaaa tattgaaagc
    15661 acgtgcagcg ccagcaacat gaccatacag gtgagcctgc gcctcatccg taaacagcga
    15721 ttctttttca ccatgcgcat caacccatgc atcgcagagt tcctcataca tcatgaaaag
    15781 ctgcgatgag atacgggcgg caaacttttt cagctccttg tcattcattc ccggcaggcg
    15841 cgcatagtgg tcacgctctg ccagaaacag caacgacgcg tcggtgttca tttcatggcg
    15901 ctgattcaca cgctcaatgc gcggccataa acgacgctga aaagtagatg tgaggaaata
    15961 aaacccgtgc accgggcttt tattgcgccg gatgtagtca tagcgtgaag taaacagcga
    16021 gcgcaaaaag taaggcaggc ggttaatcgt ggataaaaca ccttgcacct gacgcatctc
    16081 gtcacgtgta agaggtcttt cgcgcccgac agcctcgcgt ggcgcgttcc atgcataagc
    16141 accggtaaac gccttaccgg tgcctgcggt aaatgctgac ggagggacaa aacgcccgga
    16201 ggctttaacg gccatatgag ccaaaagcct ctgaacaacg cttgctgagt tgctcaacct
    16261 gcgcgtttaa atcagcaaaa gattttgcgc ttccggtcag aatatcgtga tgcatcaggc
    16321 cggaaacgag ctggcctaat ttcgggtaat aaccaaccac cgccagccat tcctgaccgg
    16381 cgtttttacc gctttccgct ctctttttct cgtggagaat aaactgaaag ctgtcactgg
    16441 taacgacata acgtttgcca atttcaatac gaatactcat gccgttctcc ggtaatgttt
    16501 gttttttgct tcaaagactg actggcagga aacacaacgc gtggctgacg gataagccgc
    16561 acgacgggca gcaggtattg gcgcgtcaca ctcttcgcaa accagcgcag aaacaccgca
    16621 atgttttacc cttgccgcgt taatctggcg ctccagtaat tcagcctgtt gttcctgaat
    16681 gaaatctacg ttgtccggca ttaccagttc cttttgtcgt tcagtttctt aaattcatca
    16741 gcgcaatagc tggcgagttc tgtcgttaat tttgtcagtt catccactga ggaaatttgc
    16801 ctgtgaaata cagcgcgttt cacaagtaaa ttgatcacat cagacaggag gtttaattcg
    16861 ctctgataaa tcgcgataac agattcagtt atgtcgcgct tttctttatc aagaccaagt
    16921 tgaataagag acaaatcgcc attttccata acagcgattt ttaaggcgtt attcagtaat
    16981 acaaccgaac gagaacagga catcaaagta cctccccgcg agacaatccg atattgtgaa
    17041 atttttccga ctcctgactg agcagctcaa ctatctccac gcgggataac tccgcctttg
    17101 tgatgtggcg aatcatggcg tcaagatgag aagaaaagcg cgtcgcagcg tcggcctgtg
    17161 cttcggttct ggcctgttgc agcagtaatg cgtatttacc gcactgattt tcagaaactg
    17221 tatgcatgac tttctccagg caaaaagaag ccccgcacga ttaagtgcgt taaaaactct
    17281 ggttaattat ttaatgcaga tattgctctg gttttaccga cgtcagaatt gtcggtgcat
    17341 actcaaaaag actgaataat tcacgtaatg cacggaataa agcatcacgc cagtaacatg
    17401 attcttcatt aattcgccag tacggctgat tgaattcttt ttctgtcaat cccgcatgca
    17461 taaataaagt acgacgctga ctgactgtta aaaaactaat atatgcatac tcacttgcgc
    17521 cgacctgacg gcgttttgag aatgcgccac gcaattcatc aattgcacaa accagtcgtt
    17581 cacgttcgac gtcgttcatt tcttcaaaac gcatcgttgc gtgacgctgt tttaactgtg
    17641 catgaaagca aaccgttagc cgttcgcgct ccatcatctg attataataa tcacatgtat
    17701 cctgccagcg agggacggca agatgcttgc caattatccg gcgcatagct gctggctgtt
    17761 tttcaacgag attgagcgtc atcactgtca tttccatacc ctccggcttt tcagaaaggt
    17821 cagagccttt tttaacggac tctgtttttt ggtgcggata atgattccct tacgcccctt
    17881 accgtgggtg atggtgaagt caatcgccct ggggctttcg ttacgcaata actgagcaat
    17941 acaacgcggc tcattcataa tcacaacccc atccacaaaa gccatgcatc acgctgttca
    18001 accggtcggt tataaaacgc ctcacgtaca gcgcgattaa actcaggaat gaaaacccat
    18061 ttttcaccgg cacgagcctt cggtttgcaa ggatcacgca attcaataat tggtaattta
    18121 tttgccttca ccatttcact gacggctgtc tttggcttcc ctaataaatc agcaaattta
    18181 tccacatgaa ccgcatcaag cggatactga atcacataat tttcagcgtc catatatggt
    18241 accctcatag gatccagccc tttctaaacc actcaaaacc gtttagatgc tggtttattc
    18301 tcaaatcaat ggaacctata taggttccag ttttgaggga atttagtccc tatataggca
    18361 ccatgtcaaa tgaaattaag cgaaaagatt aaggccttgc gtgaggctga agggctaagc
    18421 caatcaaaat tctgtgaaat catagagtta ccgctaagca cacttaaaaa atatgaagga
    18481 ggaaactttg aacccggtgg cacagctttg ctaaaaatca ctatgcatcc cacattccaa
    18541 aaatatgctc tatggcttat gacagataaa accgcgccgg acgcaggaca aatcgcaccg
    18601 gctctcgcgc acattgggcc agagtcaaca gagtccaacc actccgcgaa aaggattggc
    18661 taactctata taaagattac attttcacca tttgctacca agatggtgaa tacagcgccg
    18721 gagggctttc ttatggcaat taagaagctc gatgatggtc gctatgaagt ggacattaga
    18781 cctcgcggtc gcgacggaaa acgcatccgc aggaaattcg aaagaaaagc tgaagcacta
    18841 gcatttgagc gatacacaat cgccaatgcc agtcagaaag aatggggagg ccagcgagca
    18901 gaccgccgga ctttgagtga gttgctggac atctggtgga aatatcacgg gcaaaaccac
    18961 gagcatggaa caaaagagtt taatcatcta ctcaaaacca tcagcggcat aggtgatata
    19021 ccagtgagca ggatgagcaa aagggctttg atggattatc gttccatgcg actacgtgat
    19081 ggtatcagtg ccgcaacgat aaaccgtgac atgtaccgat tatccggcat gttcacaaaa
    19141 ttaattcaat tggatgaatt ttccgggcaa cacccaattc acggactgcc gccactggcg
    19201 gaggccaccc ctgaaatgac gttcctggaa aaagcagaaa tcgaaaaact gttaaatgtt
    19261 ttggctggtg atgacttact tgtcgcgctt ttatgtctga gcaccggagg aagatggacg
    19321 gaagttgcca cgctaaaacc agcacagatt acaaattgca gggttacctt cctgaaaacc
    19381 aaaaacggta aaaagcgaat cgtgccgatt tctgaggaac tggagaaaaa agttaaagag
    19441 gaggccagcg ccaaattatt caaagttgat tatgagaaat tttgcgggat tttacgcaga
    19501 gtgaaacctg atataccacc caatcaggca acccacatcc tgcggcatac attcgcaagc
    19561 catttcatga tgaatggggg caatataatc gcactgcaac agattctggg acatgcgagc
    19621 attcagcaga cgatggccta tgcgcacctt gcgcctgact atctgcaaaa tgccgtcgct
    19681 ctgaatccac taaaaggcgg agtgacgtta taaatttccc cctctgagtg tccacatagt
    19741 gtccacactc aagaaatttt gaaaccgctc caaaccctta cagctttcga taagtacctg
    19801 tttttacttt tgattgcatg taagtaattg ataaaaaaaa cccccacatc atgtggggga
    19861 agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct
    19921 actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc
    19981 cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta acaggcgata
    20041 catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt
    20101 ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat
    20161 tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc
    20221 tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt
    20281 actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc
    20341 gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat
    20401 catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg
    20461 caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac
    20521 gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc
    20581 gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg
    20641 cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg
    20701 acgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc
    20761 agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg
    20821 agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta
    20881 ttcgcgcaat cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt
    20941 caccgacact ggaagttgat gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg
    21001 acgggcaaac gctggagtta accggtactg agtttaccct gctctatttg ctggcacagc
    21061 atctgggtca ggtggtttcc cgtgaacatt taagccagga agtgttgggc aaacgcctga
    21121 cgcctttcga ccgcgctatt gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc
    21181 gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg cggctatctg atggtttctg
    21241 cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct
    21301 ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct
    21361 ggatagcgaa cagcgtcagg ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa
    21421 cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc
    21481 accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg
    21541 cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca
    21601 gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga
    21661 taattaccaa ctttatctga ttcgtccggc cagtagttct caatccgatt tcattaactt
    21721 actgtttgac cgcccgctat tactgctgat tgtcaccatg ttggtcagta cgccgctgct
    21781 gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga
    21841 agttgcccag ggaaacttac gccagcaccc ggaactggaa gcggggccac aggaattcct
    21901 tgccgcaggt gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca
    21961 gcagcgtctg ctttctgata tctctcacga gctgcgcacc ccgctgacgc gtctgcaact
    22021 gggtacggcg ttactgcgcc gtcgtagcgg tgaaagcaag gaactcgagc gtattgaaac
    22081 cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca
    22141 aaaaaacgcg ctggttagcg aaaccatcaa agccaaccag ttgtggagtg aagtgctgga
    22201 taacgcggcg ttcgaagccg agcaaatggg caagtcgttg acagttaact tcccgcctgg
    22261 gccgtggccg ctgtacggca atccgaacgc cctggaaagt gcgctggaaa acattgttcg
    22321 taatgctctg cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg
    22381 tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat
    22441 tttccgtccg ttctatcgga ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt
    22501 ggggctggcg attgttgaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga
    22561 cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac
    22621 tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct
    22681 gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg gggttatttt ccccacaacc
    22741 ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc
    22801 ttttcgataa cccggcggcg gagagtaaac ggtgatactg atcgtcatca aacggcatat
    22861 gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca
    22921 gcggcgcgtc cgcagacact ttccctggcg ctatcacgct atacaaccac cccaccttac
    22981 ctgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc
    23041 aaggcgagcg cggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccca
    23101 tatgaacatt actttccgtg agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa
    23161 caaacaactc cgcctgttcc ggaaattccc gcacccagta gagatagtgc tcacgaggat
    23221 aatgacacag cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt
    23281 ccagccccag ctccgtcagc atcaactcac catcaacctg gattttggca attgcgctgg
    23341 gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct
    23401 gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc agccgtgctg
    23461 ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc
    23521 cggctgtggg ga
//
LOCUS       DBKELA010000061        22740 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.061, whole genome shotgun sequence.
ACCESSION   DBKELA010000061 DBKELA010000000
VERSION     DBKELA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.061"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="ACUQR0_002410"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="ACUQR0_002410"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HHV9109411.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="ACUQR0_002411"
     CDS             691..1317
                     /locus_tag="ACUQR0_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HHV9109412.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3439
                     /locus_tag="ACUQR0_002412"
                     /pseudo
     CDS             1338..3439
                     /locus_tag="ACUQR0_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3452..4090
                     /locus_tag="ACUQR0_002413"
     CDS             3452..4090
                     /locus_tag="ACUQR0_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HHV9109413.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4154..4822
                     /locus_tag="ACUQR0_002414"
     CDS             4154..4822
                     /locus_tag="ACUQR0_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HHV9109414.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4819..5604
                     /gene="phsC"
                     /locus_tag="ACUQR0_002415"
     CDS             4819..5604
                     /gene="phsC"
                     /locus_tag="ACUQR0_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HHV9109415.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5608..6420
                     /gene="ydhT"
                     /locus_tag="ACUQR0_002416"
     CDS             5608..6420
                     /gene="ydhT"
                     /locus_tag="ACUQR0_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HHV9109416.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6432..8036)
                     /locus_tag="ACUQR0_002417"
     CDS             complement(6432..8036)
                     /locus_tag="ACUQR0_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HHV9109417.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(8162..8467)
                     /locus_tag="ACUQR0_002418"
     CDS             complement(8162..8467)
                     /locus_tag="ACUQR0_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HHV9109418.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8575..8651)
                     /locus_tag="ACUQR0_002419"
     tRNA            complement(8575..8651)
                     /locus_tag="ACUQR0_002419"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8615..8617),aa:Val,seq:gac)
     gene            complement(8656..8732)
                     /locus_tag="ACUQR0_002420"
     tRNA            complement(8656..8732)
                     /locus_tag="ACUQR0_002420"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8696..8698),aa:Val,seq:gac)
     gene            9040..10296
                     /locus_tag="ACUQR0_002421"
     CDS             9040..10296
                     /locus_tag="ACUQR0_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HHV9109419.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10337..11710)
                     /gene="mdtK"
                     /locus_tag="ACUQR0_002422"
     CDS             complement(10337..11710)
                     /gene="mdtK"
                     /locus_tag="ACUQR0_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HHV9109420.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11925..12566
                     /gene="ribE"
                     /locus_tag="ACUQR0_002423"
     CDS             11925..12566
                     /gene="ribE"
                     /locus_tag="ACUQR0_002423"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HHV9109421.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12606..13754)
                     /gene="cfa"
                     /locus_tag="ACUQR0_002424"
     CDS             complement(12606..13754)
                     /gene="cfa"
                     /locus_tag="ACUQR0_002424"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HHV9109422.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14045..15256)
                     /gene="punC"
                     /locus_tag="ACUQR0_002425"
     CDS             complement(14045..15256)
                     /gene="punC"
                     /locus_tag="ACUQR0_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HHV9109423.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15369..16301
                     /gene="punR"
                     /locus_tag="ACUQR0_002426"
     CDS             15369..16301
                     /gene="punR"
                     /locus_tag="ACUQR0_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HHV9109424.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(16298..17323)
                     /gene="purR"
                     /locus_tag="ACUQR0_002427"
     CDS             complement(16298..17323)
                     /gene="purR"
                     /locus_tag="ACUQR0_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HHV9109425.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17622..17711
                     /gene="cydH"
                     /locus_tag="ACUQR0_002428"
                     /gene_synonym="ynhF"
     CDS             17622..17711
                     /gene="cydH"
                     /locus_tag="ACUQR0_002428"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HHV9109426.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17877..19046
                     /locus_tag="ACUQR0_002429"
     CDS             17877..19046
                     /locus_tag="ACUQR0_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109427.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19192..19773)
                     /gene="sodB"
                     /locus_tag="ACUQR0_002430"
     CDS             complement(19192..19773)
                     /gene="sodB"
                     /locus_tag="ACUQR0_002430"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HHV9109428.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19901..20728)
                     /gene="mepH"
                     /locus_tag="ACUQR0_002431"
     CDS             complement(19901..20728)
                     /gene="mepH"
                     /locus_tag="ACUQR0_002431"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HHV9109429.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            21063..21410
                     /gene="grxD"
                     /locus_tag="ACUQR0_002432"
     CDS             21063..21410
                     /gene="grxD"
                     /locus_tag="ACUQR0_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HHV9109430.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21524..21949)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_002433"
                     /pseudo
     CDS             complement(21524..21949)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            21957..>22740
                     /locus_tag="ACUQR0_002434"
     CDS             21957..>22740
                     /locus_tag="ACUQR0_002434"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HHV9109431.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNH"
BASE COUNT         5908 a         5289 c         5872 g         5671 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat taactggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc
     2101 acgtaaaggg ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc
     2161 gccaggcaat cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc
     2221 ggcggcagag aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat
     2281 gacccaaatg aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac
     2341 ttgtgtagca aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga
     2401 taaagacgat ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg
     2461 cctatggtgt aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt
     2521 gttcaagcgt gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc
     2581 gggtgacgtt aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct
     2641 gagtcacctg gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta
     2701 ctggggctac gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa
     2761 tgaagcgtca gcgcaggtcg gttccattgt taactgtatg ttcaaccgtg actgcatgac
     2821 gcatacccat atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc
     2881 gaaagaactt tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa
     2941 tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt
     3001 cactctgtgc aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg
     3061 gggcgatctg gcgctttaag ccaaattctt caaagcgatc accggcgaag agatgactca
     3121 ggaaaaatta gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa
     3181 accgatgcaa accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga
     3241 caaggatccg cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat
     3301 gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc
     3361 aacccgcgaa acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca
     3421 caatctactg cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc
     3481 gaaagtcagc gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt
     3541 tgatacggta accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc
     3601 tgcggctggg cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga
     3661 agaggaactg tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc
     3721 ttgtatgacg ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta
     3781 cgcggcgatg tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt
     3841 tcgttttgag tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc
     3901 atattacttc caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga
     3961 atatgccgat atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg
     4021 atttatgacc tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca
     4081 gaacccctga aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt
     4141 aggggataat cagatgtcct ttactcgacg caaatttgtt ctggggatgg gaacagtaat
     4201 cttttttact ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg
     4261 gtatgccatg attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg
     4321 ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt
     4381 caccgataac gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga
     4441 agatgctccg tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat
     4501 tgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta
     4561 ccaggttcgc taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga
     4621 gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt
     4681 gatctttggt cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta
     4741 tcaatatcag ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt
     4801 gattaaaaag gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta
     4861 gcgaactatg tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga
     4921 gtattgctgg ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc
     4981 gtgaagaaat cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga
     5041 ttatggcact ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat
     5101 cactttgcga tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc
     5161 ggatttttgt tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat
     5221 ggtcaccact atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga
     5281 ttttatttgt ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct
     5341 aaatttaatc ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg
     5401 ttactattga cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc
     5461 gtaagatact ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc
     5521 ttcggtcatc tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg
     5581 gtcgatggct atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg
     5641 cgtatcggag cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat
     5701 gccgatattc gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt
     5761 gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct
     5821 atgcaaaaac tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc
     5881 gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat
     5941 gccgcgcaaa ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt
     6001 cacattggca gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata
     6061 agcagcgctg gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca
     6121 ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc
     6181 gcaaacgcca gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat
     6241 tcaacggtag tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc
     6301 gcactggcat atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac
     6361 aatattcgaa ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga
     6421 tagctttacc tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc
     6481 gcccgagcca tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg
     6541 tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc
     6601 tgtaacgtat aatcttcgcc aacatcaggg atttcatccc ctgttttctg taattgctcg
     6661 cgtagcccag ggaaaggaat atctttcact ttaagcggac gctgtccgcg ggcatcaata
     6721 aaaacgtcaa acgagtagct gttgtcttcc gttttcagga cggtgcgcga ctcattaatt
     6781 tccatttcgt agtcttcacc gagggcgaga atatgaatga tccccgcttc acgtaaggcc
     6841 agtagtcgac gaatagactc tgaagggatt gccgcataat tatcgatgaa aaccctggca
     6901 aggcctttac tgaaccgttt atggtcgtgt tcattcagat gaggaacaat ttcctgtacg
     6961 gcttcatgca ggcgcagaat gacataacgc cagggaacag tatgtttttc tcgtttattg
     7021 cgttcgactt cctggagatt tttttctgcc cagtcaaatg ggtcgcgttg tttgcgctcg
     7081 gcaaaccagg cttgagcaaa ggaatcgaca ttcaggctct ctaaggctat gcgttggctc
     7141 cagtctggat cagcaaactt gatttcctct actatcaatc taaatacccg atccaaaagg
     7201 ccctcttcac ctttttgaat ctcagcattt aatgcctgat cggtgacgat gtgtaagggc
     7261 tcgtagggaa tagggcaata gaaatcggct tcgggtaaaa tacccgtgcg cgacatcaac
     7321 gtaatattta gcttttcact tgcgttatcg cggtgaaaaa cgacgtgttg tttatcatct
     7381 tcaatgaacg aaccatgctg aatagccact gccattgccg catccagtcc gctcaaggat
     7441 gttcccataa tacccacgtt acacgcatcg acctttgctt ccatcaggcc tgaccacggg
     7501 ctgggaaaat acgttctggt tgcttcttct tcatcaggcc agacgtgacc cgtggcaatc
     7561 accgctaaat caaacgtctc gctgggtaaa tcctgatttg tagcgagcat gacgccagca
     7621 tttgtaattt gcagatcggt aacctggcat gattcataaa cagccactgc aaatttttgc
     7681 tgtcgtgcct ggtctactaa tcgtaaaaat tgatcgcgga aatattcgcc cagcagaatt
     7741 cgcggtaaaa actgacgatc gtgcaaggtt tcttttttaa cgccataacg ctggagatgg
     7801 ctggcttctt gcttttgtag ccattcgaga tacgtacaat taatcggcgg tatttcaata
     7861 ctggcaatat ttgccagcat catttttgag ttttcctcat cactgtatgg catcccgaca
     7921 ccggcctcgt cagcctgctc gaagatagaa atagaaagtg gagtttgttg ctgtagaagc
     7981 gagaataagg tgtagatccc cgtaggcccg gcacccacaa tagcaatttt tttcattgtt
     8041 acttacctgt acgcattctt tcgaatttag tgttatcaga taagtttggt tgagattttt
     8101 agaatttgag aaatttcaga cagggatgaa aagggcgcta accgcccttg attaaattct
     8161 gtcaggcgag ttttgcctga ttgatttgac taagtggctc attgacatcg aaaactttgg
     8221 cgacaacttc ctcaacgccg agatttttca gtcgggcagt atgtttttcc agataggcaa
     8281 gggcgctttt ttcatcagtg aaaaggtaga tcccaccggc ttcgtggttc ttttcacttt
     8341 ctgtccatac cttccacaga aaaccaggtt cctgattaat cgactcagca agtggtttaa
     8401 gctgctcagc cattgcgtcg ccaaatgggc cattaaaagc aaaatgaagt tgtaacaaag
     8461 ttgccatagc gattctcctt ctgataacgc atacgaagac agtgtaaatc ttttaacgta
     8521 ttggatacag agaagaaaca gagggaaaaa ggaaaagacc agattaagaa aatctggtgc
     8581 gtccgagtgg actcgaacca ccgaccccca ccatgtcaag gtggtgctct aaccaactga
     8641 gctacggacg caggatggtg cgttcaattg gactcgaacc aacgaccccc accatgtcaa
     8701 ggtggtgctc taaccaactg agctatgaac gcaacgttgt aggtgacaac ggggacgaat
     8761 attagcggca gagtggaaag gtggcaagag gcaaaacgta attttctgcg ctatttcgac
     8821 cgtttgcaga gcttttaagc aaattggcta tattttgttg atttgcaagg gcgattttta
     8881 ttcaggatcg catttacatc tgatacaacc caataattcg tgtatcagag tttaaacaga
     8941 gttgtttgcc accatctcaa atctgtcgtg gataaatcct ccgtagcctt tacacttagg
     9001 tagcaataaa ggcacacaga tgcagcaaag gaataaatca tgggatctga tgcgaaaaac
     9061 ttgatgagcg acgggaatgt gcaaattgtt aagaccggcg aggtccttgg cgcgacgcaa
     9121 cttactgaag gcgagttgat tgttgaagct ggcggaagag ccgaaaatac cgtggtcacg
     9181 ggggctggct ggttgaaagt ggcaaccggt gggatcgcca aatgcacaca gtacggtaac
     9241 aatggcacgc tatcggtcag cgacggtgcc attgccacag atattgttca gtccgaggga
     9301 ggcgcaatta gtctctctac gctcgctacg gttaatggcc gccatcccga aggtgaattc
     9361 agcgttgatc agggttatgc ctgcggtttg ttgctggaaa atggcggtaa cctgcgtgta
     9421 ctggaaggac atcgcgcgga aaaaatcatt ctcgatcaag agggcggcct gttggttaat
     9481 gggacaacct cagcggtcgt ggtagatgaa ggtggtgaat tgttggtgta tccaagtgga
     9541 gaagccagca attgtgagat taatcagggc ggcgttttta tgctggccgg gaaagccagt
     9601 gatacgttgc ttgctggtgg caccatgaat aatctcggtg gtgaagactc tgacactatt
     9661 gttgagaatg gatccattta tcgtctgggg acggatggcc ttcagctcta cagttccggt
     9721 aagacgcaaa acctgtcggt gaatgtgggg gggcgggctg aagtgcatgc cggtacgctg
     9781 gaaaatgcgg taatacaagg tggaacagtg atcctgttgt cacccaccag cgcggacgaa
     9841 aattttgtcg tagaggaaga tcgcgcaccg gttgaactga ccgggagtgt tgcattactg
     9901 gacggcgctt caatgattat tggttatggc gcagagctgc aacaatcaac gattactgta
     9961 cagcagggcg gtgtgttgat tctcgacggc agtacggtaa aaggtgacag cgtcactttc
    10021 attgttggta acatcaatct gaatggcgga aaactgtggc tgatcactgg tgcggcaacg
    10081 catgtgcaac tgaaagtgaa acgcctgcgc ggagagggag cgatttgcct gcaaaccagt
    10141 gcgaaagaaa tttcacctga cttcatcaat gtgaaagggg aagttaacgg ggatatacgc
    10201 gttgagataa cagatgccag tcggcaaact ctgtgtaacg ctctgaaatt acagccagac
    10261 gaagacggga ttggcgcaac gctccagcct gcgtaaaaac acaggcgcaa cccgtgaagg
    10321 ttgcgccttg tctttattag cgggacgctc gttgcagaat gatggctgac ggcatacgtt
    10381 gcaggaaccg catacgcagc atcatcataa tggctgccga tgtcaggccg ataataaagc
    10441 ctatccagaa gcctgctggc cccataggtt caacgaccag atcggtcagt gccagaatat
    10501 agccgcttgg caagcccagc acccagtaag ctgtaaaggt gatatagaaa atggaacgcg
    10561 tatctttata accacgcaaa atcccactgc caatcacctg gattgagtca gaaatctgat
    10621 ataccgccgc cagcaacatc aaatgcgcag ccagcgttac aacctcggga ttgtcgttgt
    10681 acaacagggc gatttgctcc cgcagtgaaa ccgtgaaaat ggccgtcagg gttgccatac
    10741 agacacccac cataagcccg gtccgcgcag cggtttgcgc atccagcgtt gagccctgac
    10801 ccagacgata acctacgcgg atagttaccg ctgccgccag cgacattgga agcacaaaca
    10861 tcagtgaact aaagttcagg gcaatctggt gtcctgcgac atcaacaata ccgagcggag
    10921 acactaacag agccacgacg gcaaacagtg tcacttcaaa gaacagtgcc agcgcaatcg
    10981 gcaaaccgag ttgaatcagt cgtttcataa ccgcaggctc gggcttagcg gtgccttttt
    11041 cgttacgaat atcgcgcatg gagcgggcgc gtttaatgta agaaaccatg gcaaggaaca
    11101 tgacccaata caccgccgca gtagccacgc cacaaccaac gccaccgagc tcaggcatac
    11161 cgaaatgacc ataaataaag atatagttca ccgggatgtt caccagcagg ccgataaagc
    11221 ccattaccat acccggcttg gtttttgcca gaccttcaca ttggttacgg gcaacctgga
    11281 agaacagata tcccggcgcg ccccacaaca acgcacgcag ataacccacg gctttgtccg
    11341 ccagagccgg atcgatgttt tccatgtagc ggataatgta acctgcattc cacagcacca
    11401 gcataatgag aacggaaaca aaacctgcca gccagaaacc ttgtcgcacc tgatgcgcaa
    11461 tgcgctcacg tcgaccggaa ccatttaatt gcgcgataac cggcgttaat gccagcagca
    11521 gtccgtgacc aaagaggatc gccggaagcc agatagaagt accgatagcg accgccgcca
    11581 tgtcggtggc actatagccg cccgccatca cggtatcgac aaaacccatc gcagtttggg
    11641 cgatttgcgc gagaatcacc gggattgcta atgctaataa cagacgcgct tcactgatat
    11701 acttctgcac gtgaacacct tttatttgta gttatatgag agactaaaaa gccgcctgaa
    11761 tgggcagcaa aaagaagaag caggggaaat tcagtctatt gtagcgagga tttattaatt
    11821 ctccagtgaa aaaatagcca ttcttacccc ttcactggta agggcgtttt ccacctgcta
    11881 ttgtgctgaa cagaatgtct taactgattt caggagttgt aagtatgttt acggggattg
    11941 tacagggcac cgcaaaactg gtgtcgattg acgagaaacc aaattttcgt acgcatgtgg
    12001 tggagttacc cgaccacatg ctggacggcc tggaaaccgg tgcttccgtg gcgcataacg
    12061 gttgctgcct gaccgtgacg gaaattaacg gcaaccatgt cagttttgac ctgatgaaag
    12121 aaacgttacg cattaccaat cttggcgatt taaaagtggg ggattgggta aacgttgagc
    12181 gtgcggcgaa attcagtgat gaaattggcg gacacttaat gtcaggtcat attatgacca
    12241 ccgctgaagt ggcgaaaata ttaacctcgg aaaataatcg ccagatctgg tttaaagtcc
    12301 aggatagtca gttgatgaaa tatatcctgt acaaaggatt tattggcatc gacggtatta
    12361 gcctgaccgt cggcgaagtc acgccaacgc gtttttgcgt ccatttaatt ccggaaacac
    12421 tggaacgcac gactcttggg aagaaaaaac ttggcgcacg cgtcaacatt gaaatcgatc
    12481 cacaaactca ggcagtggta gatacggtag aacgtgtgct ggcggcacga gaaaatgcca
    12541 tgaatcaacc aggtacagaa gcctgatctg aactcgcccc cgaagagggg gcgatagaat
    12601 agcctttagc gagcgactcg aaggccgttt tcaacaccgc gtgagaacac cacctgccag
    12661 agctgaatat cacgggcgcg gaaagcacct gcgcaggcat tcagataata ggtaaacatc
    12721 cgtttaaagc gttcactgta gttatccgca atttctggcc atgcggcgag gaatcgttca
    12781 taccacgcca tcaacgtagt atcgtaatca gcaccgaagt tatgccagtc ttccatcaca
    12841 aagtggggtt cgctggattg agcaatctgg cgtacagagg gcaggcaacc gttcggaaaa
    12901 atatatttat taatccaggg atcaacattc agatcggttt ttttcgaacc gatagtatgg
    12961 agcaggaata taccttccgg tttcaaatta cgatccacca ccgcaaaata ggtatcgtaa
    13021 tttttcggtc cgacgtgctc gaacatcccc acagaaacaa tacgatcaaa ctggtcgttc
    13081 aggtcacgat aatcttgcag caaaatggtg acatccaggc ctttacagcg ttcctgagcc
    13141 attttttgct gttcggcaga aatggtgacg ccgaccacgc ttacgtcata gttagatgcc
    13201 atgtagtgcg ctagtccgcc ccagccgcag ccaatatcca gtacgcgcat ccctggtttt
    13261 aactgcaatt tttcacaaat cattttgagc ttcgcctgct gggcagattc cagattatcg
    13321 gcatctttcc agtaagcgca ggaatattgc atgaagggat caagcatgcg gctgaacaag
    13381 tcattaccca aatcgtaatg ctccttgccg actatccagg cacgtttttt actctgcaga
    13441 ttgaagagac gagcgccggc aatacgcagc gtgtctttga aatgatgggg gagttggttc
    13501 tcgagacctg cgcgtaagac tttgctaaaa aacatatcca gtcggtcaca ttcccaccag
    13561 ccatccatat aactttcgcc taaccccaaa gagccttcct gcagaacgcg tttaaaaaaa
    13621 tcggggtttt tcacacgaat atccgccggg gcagaaccgt taatggctat accggcacgg
    13681 ctaagtaatt cgttggcgat acggtaccag ttgtcatccg gtacactgac ttcttctata
    13741 cacgatgaac tcatagtttc tccatcactg gtcggtgatc agaaccttaa aacagcgtag
    13801 acgctttttg ggctttgtga gaaatctcgc agaaaaaacc gcgagtctat aatccgacgt
    13861 agaacagagg gaaaggagga acccttgccg aaatgaccat ccatagtgag tcgggaacga
    13921 tcctgtcccc gtaaaaacat aatatttatc gtagctttaa caactcgaaa ttagtatagg
    13981 ccgctaattt caatcataca acaaaatttt gttagcagcc taagtataag tatatcgata
    14041 tagatcagtg tgattcgcta tgagcgactt cggcattgcc atgattcggg cagtcaactt
    14101 cttcacaacg ttgcatcatg taacccagcg cgaccagcac tactgttgat aacatcacgc
    14161 tggtggtggt gagcaatggc gtgctgatac taataagcca ggaaactacc agacttgcga
    14221 ggaagcacag acccagttgc agagtgttct gcaacgctgc ggcgcgaccg gttgcatgtg
    14281 ggaagggacg cagcgcctgg gcgacaacaa tagggtagat cgcgccattg gcaatcgcca
    14341 tcacacagaa tgggatcagg atttcgacca gcgacacatg gctaatgaag cccgcagccc
    14401 aggtcgcaat gacgctgaca gcaaacagca ccagcaacca cggtaataac tgcttgcctt
    14461 gccatttctg cagtgcggcg cgacagccat aaccaccaat cagaaacgcg atagtttgcg
    14521 ggacataact taaaccaata actgccgggc tgtagcccat ttcactaagg atgaacggtg
    14581 aaccggtcag ccatgcaaaa aaactggctg aacaggctgc gtatatcagc acgttgccgc
    14641 gataggtttt agaacgtagc aggtcggtaa aggtcagacc atcctgacta ttgttacggg
    14701 ccttcgtcgt gggtttgagc cagaaaatag gcagaatcag caccacggta atggcaaaca
    14761 gggtggcgaa aatcgcctgc caggaaaaat ggaccagcag ccagcttcct aacaggggag
    14821 ccagtgccgg agacagaccc accagcggca tgatggtcgc aaaaatacgg ttgactttct
    14881 gtgaaggata ataatctgtc actaatgctt gccagataac cgccgcggcg cagaccccca
    14941 cagcctgtac aaaacgcaat accagcagcg tggcggcgtt ttctacccac agcatcccca
    15001 gactacctaa cgcaaaaatt gtcaggccga ttaataacac cggtttacga ccataacggt
    15061 cggagagtgg cccccacaga agctgggctg cggcaaaacc ggcaaggaac agactaaggc
    15121 tggcactgac agcagacgca ggcgtttgca ggtcggcctg tatggcggcg aaagcaggca
    15181 gatacatatc ggttgccaga aaaccgagta cgctcaaacc cgccagccag actaaaaatc
    15241 ttttcccagg ttgcatgtat attctctaaa tctctcaatt aaacaccgcc gcaaagtgta
    15301 gggagtgaaa ttagtgttgt gaaacggtaa tatttgatga ctggtttcaa aaattttgca
    15361 ggcagactat gtggtcagaa tattcactcg aagttgttga tgcggtagcg cgtaatggta
    15421 gttttagcgc tgcggcacag gagctgcatc gtgttccttc tgcggtcagc tataccgtgc
    15481 gtcagctgga agagtggctg gcggtaccgc tctttgaacg acggcaccgt gatgtggagc
    15541 tgaccgctgc tggagcgtgg tttctcaaag aagggcgctc tgttgtcaaa aaaatgcaga
    15601 tcacccgcca gcaatgtcag cagatagcga acggctggcg cggtcagtta gctatcgcag
    15661 tggataatat tgtcaggcca gaacgtacac ggcagatgat cgttgatttt tatcgccatt
    15721 ttgatgatgt cgaacttctt gtctttcagg aagtgttcaa cggtgtctgg gatgcgcttt
    15781 ccgacgggcg cgtggaactg gcaattggcg caacacgcgc gattccggta ggcggtcgtt
    15841 atgccttccg ggatatgggg atgctaagct ggagttgcgt tgttgccagt caccacccgc
    15901 tggcgttgat ggatggcccg ttcagcgatg atacgttgcg caactggccg tcgttggtgc
    15961 gcgaagacac ctcgcgaacg ctacctaaac gtattacctg gttgctggat aaccaaaaaa
    16021 gacttgtcgt acctgactgg gaatcatcgg caacctgtat ctcggcagga ttatgcatag
    16081 ggatggtgcc aacacatttc gccaaaccgt ggcttaatga ggggaagtgg gtagcacttg
    16141 aactggaaaa ccccttcccg gattcggcat gttgtctgac atggcagcaa aatgatatgt
    16201 cgcctgcgtt aacctggctg cttgagtatc tcggcgatag tgagacgctg aataaggagt
    16261 ggctacggga gccggaagag actcccgcaa cgggtgatta acgacgatag tcgcggaacg
    16321 ggccgtcagc cacggagcgg cgttcaatca aacgcggatg cacttcaatg gactgcggtt
    16381 cttcacgttt gttgacgata cgatccaaca gcatgttgaa cgctgtttca cccagcgaat
    16441 cttttggctg atggatcgtg gtcagcgccg gcgtaaaata gcgcgcgttg cgcacgttat
    16501 cataaccgat cagcgaaaca tcctgcggga cgcgcaggcc catttcatca gcagcacaaa
    16561 gtgcgcccat tgccatgata tcgccaccac agaagacggc agtagggcga tgcggctgcg
    16621 acaggatttg ctgcatggcg cgataaccgg attcaggttc aaagtcaccc tgcacaatcc
    16681 agctttccgg caccttgatc atcgcttctt ccatcgcctt cataaaaccg gcaaggcggc
    16741 ctgcgccggt gttacgttcc agcgggccgg ggatgacgcc gatttcgcgg tgaccgcgtt
    16801 caatcagata acgcccggcc atgtagccgc cttcgaacgc gttatcaatg accgcatcgg
    16861 tgaagtcagc ttttgcttca ccccagtcca tcaccaccat tgggatatgg cgatactctt
    16921 ccagcatcgc cagcaacggc tctgggtact cagaacacat caccagcaga ccatcgacgc
    16981 gtttttgcgc catcatcgac agataagccc gctgtttctc aagattgttc cacgcattac
    17041 ccagaatcag ggtgtaacct ttctggaagc aatttttttc aactgcttca atgatctcgg
    17101 caaaataggc cgcttcgctg ctggtcgcca gcaaaccgat agacttggtg tggttaacct
    17161 tcaggctacg cgccaccgcg ctaggggagt agtgtaattc tttaatcgct gcccacacgg
    17221 cgttgcgcgt ttcttcagcg acgaaacgtg ttttgttgat cacgtgtgac acagttgtag
    17281 tggaaacgtt tgctcgtttc gctacatctt ttattgttgc cattccattt cactccagac
    17341 cctaacttca gctcctgcaa aatcgcaagg taaacgtttg cctttacaca ccttacgcac
    17401 aacaaaagtg ttgcggtacg ccggaaaaac gacacggagc gtcaggaagg ggtcaatggc
    17461 cagtacgcaa atagtgtaag cgtggaattt tggccgatct tgacgaaaag gggaagtggt
    17521 aaaaacactt atcattataa atgacgggag attttttcat cacagtgtgt aaaaatgcgt
    17581 aatatcactt tttgtgtggt aataaaaaag gagaaaactt gatgagcacc gaccttaaat
    17641 tttcactggt aacaacgatt atcgtcctcg gtttgatcgt agccgtgggt ttgactgccg
    17701 cgctgcactg atttcctctc gagggagcgc gttctccctc gcatctcctt cctgtttgtt
    17761 agcaaaatgg caaaagtgtt ttctcttttt gttattttca tttttttgtg attgctgtca
    17821 tgctttcgcc actttaatga atatcgcgta atgacgcgaa gaggtggaga taaggcatga
    17881 aaattaacta tccgttgctg gcgctggcga ttggcgcgtt tggtatcggg acaacggagt
    17941 tctcgccaat gggcttgttg cccgtcattg cgcgcggtgt ggatgtctcg attcccgctg
    18001 ccggaatgtt aatcagtgcc tatgcagttg gcgtaatggt gggcgcgccg ctgatgacgc
    18061 ttctactttc tcatcgtgcc cgccgcagtg cgttgatttt cctgatggca attttcacgc
    18121 taggcaacgt tctttccgcc atcgcgccgg attatatgac cctgatgctt tcacgtattt
    18181 tgaccagcct gaatcacgga gcattttttg gtttgggttc agtcgtggcc gcaagcgtgg
    18241 tgccaaaaca taaacaggcc agcgcagttg ccactatgtt tatggggtta accctggcaa
    18301 atatcggtgg cgtgccggcg gcgacctggt tgggtgaaac catcggctgg cggatgtcat
    18361 ttctggcaac ggcggggctg ggagtgattt caatggtaag tctgttcttc tcattaccta
    18421 aaggtggtac aggggcacga cctgaagtga aaaaagagct ggcggtatta atgcgtccgc
    18481 aggtgctgtc tgcattgctg acgacggtac tgggagctgg tgcaatgttt accctctaca
    18541 cctatatctc tccggtactg caaagtatta cccacgcaac accggtgttc gtcacggcaa
    18601 tgctggtgct gattggtgtc ggattctcta tcggtaacta tctcggcggc aaactggcag
    18661 atcgttcagt taacggcacg ttgaaaggct ttttgttgct gctgatggtg attatgctgg
    18721 caatcccgtt cctggcccgc aatgagttcg gcgcagctat tagcatggtg gtgtggggcg
    18781 cagcaacctt tgcggttgta cctccgttac agatgcgcgt gatgcatgtc gccagtgaag
    18841 cgccaggtct gtcttcatca gtcaatattg gtgcctttaa tcttggaaat gcgctgggag
    18901 cagctgctgg tggtgcggta atttccgctg ggctgggata cagctttgtg ccggtgacgg
    18961 gggcgattgt cgcgggactg gcattattgc tggtgtttat gtcagccaga aaacaacctg
    19021 aaacagtttg cgttgctaac agctaattaa acaaaaacgc agaaggataa ccttctgcgt
    19081 tacagttgct gacaacgtgc gatttatctt gccgaatgcg ctgttaacgc ctcatccgcg
    19141 ttggcatggt aagcgaagcg catcaggcaa tgctgcattt gccatcagtt attatgcagc
    19201 gagatttttc gctacgaatt cccagttcac cagcgcccag aagtgctcca gatagccagg
    19261 acgtgcattg cgatagtcga tgtaataagc gtgttcccag acatcaacgg tcagcagcgg
    19321 agtcgcatcg gtggtcagcg gagtacccgc gttagaggtt gaaacgatag ccagtttgcc
    19381 atcgctgttt ttcaccagcc aggtccagcc agaaccaaag tttttgatcg ctgcatcagt
    19441 aaactgcgct ttgaaatcgg caaagctgcc aaaagatgcg gcgatagctt cagcgacttt
    19501 tccagtcggt tcgccaccgg cgttcggtgc caggcagttc cagtagaaag tatggttcca
    19561 gacctgagct gcgttgttga atacgccacc ttcagagctg cgaataatct cttccagtga
    19621 tttaccttca aacgcggtac ctttaatcag gttgttcagg ttagtgacat aagtctgatg
    19681 atgcttgccg tagtgatact cgatagtttc cgcagaaatg tgcggtgcca gagcatcttt
    19741 agcatatggt agtgcaggta attcgaatga cattgctact ctcctttatt attaatttgc
    19801 atacgtacaa tagccttatt gtgcgtatgg atagggtagc aaattaaaag gtgagacaaa
    19861 agatgaactt accctgttgc cgtaacaaca gggtaaagtt ttagcgaagt gtttttgggg
    19921 tcattacccg acgagcgcca acatagtggc gctgccagta gtcttcactg agagaagtga
    19981 tttgaatttc ctgacctgtg cgcggtgact gaataaattt gccgttgccg acatacacgc
    20041 cgacatgatc ggctgtaccg cgtccctgag tacggaaaaa gaccaggtcg ccgtttttca
    20101 gctcactacg ttcgataggt cctgcatcac gcaggtgata catttcattc gccgtacgcg
    20161 gaatacgaat tttcaccaaa tctttataag cgtaataaac caggccgctg caatcaaaac
    20221 cggtatgcgg tgagctgccg ccccaacgat atggcttacc aatttgctgc atcagtttat
    20281 tcattgccac tttcgtggct ttttgcacct tcgctttgtg cgcgtcggca agggttcctg
    20341 acgcggcatt tttgactttc acacaatgcg atttatagcc cttacgtttg gtacactttt
    20401 cggtaaagct ggctgaggcc gttttattga cggcgtgttt gctgcgagtt ttggcgtttt
    20461 tagaagcggt aatggaagat tttttaacag aagaagcggt tttgcttttg gtcatacttt
    20521 tactggcggc ttttttcgct gttttctggc tggctttctg ggtctttttg gtggttgccg
    20581 tgcttttctt tttatctgct tttttggtga tatgagtggt agcagaactc tccctggctt
    20641 gctttgaagc atgggccata ggcgttaaag gcagggtggt aaaaagtaaa gcacagagcg
    20701 tgatcgaaat acggtttatc cgcgccactg agcgtccctc tgttaaggca ggtctatcat
    20761 gcctgcgtct gatttacaaa acttgatgat tctaatgcat agaagaagag aaagttaata
    20821 gcttttattg ttaaaaaata cgtttcgttg gcgagcgcaa aaaaatgcag cagtgaaagc
    20881 taaaaatggt tataaatacg ccagtttgcc gcgcatcaga gaagaaacga atatttgaaa
    20941 tgcaacaaat agagcaaagg gataaaatag caaaagcgtg atgaaaatgt tattttccga
    21001 tgccgcactg acccgctaca atgtcagatt actgccgtaa agaagtttga ggaagcaaga
    21061 caatgagcac cactatcgaa aaaatccaac gccagattgc tgaaaacccg atcctgctgt
    21121 acatgaaagg ttcaccgaaa ctgccgagct gcggtttctc tgcccaggca gtccaggcgc
    21181 ttgccgcatg tggcgaacgt tttgcctatg ttgatattct gcagaatccg gacattcgtg
    21241 cggaactgcc gaaatatgct aactggccga ccttcccgca actgtgggtt gacggcgagc
    21301 tggtcggcgg ttgtgatatc gtgatcgaaa tgtatcagcg tggcgaactg cagcagctga
    21361 tcaaagaaac tgccgctaaa tacaagtctg aagagccgga cgcggaataa tccgtcctgc
    21421 aatactgacg gatgcgaacg ggcatccgtc agtcctttat ccggtgcgtc gtaaaacctc
    21481 gtccttcagg gcggggatat aaggcgcggt tttccacctg accaggtgtt tgctgttgtt
    21541 caatgtactg gcggatgacg gatattggcg caccttcgca gctactggca aaatagccag
    21601 gactccacaa aacgcctttg tagtaatacc tgactgcaat atctggtcga tctcgtcgca
    21661 gtaacctatt cgatatgcct ttgaggctgt ttaccagact ggatatcgcc agtttgggag
    21721 gatagttaat taacaaatgg acgtgatctg gttcgccatc catttcaacc agttcagctg
    21781 caaaatcagc acatacattt gaaaagtaag tgcgtagttt ttctgtcgtg tcatggtcaa
    21841 aaatctggcg tcggtatttg gtgacaaaga ccaggtgaac atgcatcagg aaaacacaat
    21901 gccttccacg ccggatatcg gtttcttttt tcacagacca aagtatcatt tgacctgtga
    21961 aacgattaca ggcatttaaa ttccagttaa gaccaggtgg tcaacaggag cgtgaaatga
    22021 ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc tctggcgctt cagaatgaga
    22081 atcatgaggc cggtaataaa tacatccctt acgggaaaat ggcttcctgg ctggttgagt
    22141 ggaaaaacgc cactgaaacg caatggctta aagattcgcc ctcacagcca ttgcaacagt
    22201 cactgaaaga ccttgagcgg gcttacaaaa acttcttcca gaagcgggct gcttttcccc
    22261 gattcaaaaa gcggggacag aatgctgcat tccgctaccc gcaggacgtt aagctcgatc
    22321 aggaaaacag ccgtattttt ctgccgaaac ttggctggat gcgctaccgg aatagccgtc
    22381 aggtcacggg agttgtgaaa aatgtcactg tcagccagtc ctgcggtacg tggtacatca
    22441 gtattcagac agaaagtgaa gtatcaactc cggttcaccc ttcagcatca atgatcgggc
    22501 tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt gagcctgtaa
    22561 acagttttca aaaaaaccag aagacgctgg cgagacttca gcgacagtta agccggaagg
    22621 tcaaattcag caacaactgg cagaagcaga aacgcaaaat acagcgactg cattcctgta
    22681 tcgcaaatat ccgcagggac taccttcaca aagtcacaac gaccgtcagc aaaaaccacg
//
LOCUS       DBKELA010000062        22550 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.062, whole genome shotgun sequence.
ACCESSION   DBKELA010000062 DBKELA010000000
VERSION     DBKELA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.062"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            177..470
                     /gene="yicS"
                     /locus_tag="ACUQR0_002435"
     CDS             177..470
                     /gene="yicS"
                     /locus_tag="ACUQR0_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HHV9109432.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(511..1701)
                     /gene="nepI"
                     /locus_tag="ACUQR0_002436"
     CDS             complement(511..1701)
                     /gene="nepI"
                     /locus_tag="ACUQR0_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HHV9109433.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1912..2364)
                     /locus_tag="ACUQR0_002437"
     CDS             complement(1912..2364)
                     /locus_tag="ACUQR0_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HHV9109434.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="ACUQR0_002438"
                     /gene_synonym="yicO"
     CDS             complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="ACUQR0_002438"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HHV9109435.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3926..5692
                     /gene="adeD"
                     /locus_tag="ACUQR0_002439"
     CDS             3926..5692
                     /gene="adeD"
                     /locus_tag="ACUQR0_002439"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HHV9109436.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="ACUQR0_002440"
     CDS             complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="ACUQR0_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HHV9109437.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="ACUQR0_002441"
     CDS             complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="ACUQR0_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HHV9109438.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="ACUQR0_002442"
     CDS             complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="ACUQR0_002442"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HHV9109439.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="ACUQR0_002443"
     CDS             complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="ACUQR0_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HHV9109440.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="ACUQR0_002444"
     CDS             complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="ACUQR0_002444"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HHV9109441.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="ACUQR0_002445"
     CDS             complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="ACUQR0_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HHV9109442.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="ACUQR0_002446"
     CDS             complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="ACUQR0_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HHV9109443.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13514..13603
                     /gene="tisB"
                     /locus_tag="ACUQR0_002447"
     CDS             13514..13603
                     /gene="tisB"
                     /locus_tag="ACUQR0_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HHV9109444.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13727..13801)
                     /gene="ysdE"
                     /locus_tag="ACUQR0_002448"
     CDS             complement(13727..13801)
                     /gene="ysdE"
                     /locus_tag="ACUQR0_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HHV9109445.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            13883..15067
                     /gene="emrD"
                     /locus_tag="ACUQR0_002449"
     CDS             13883..15067
                     /gene="emrD"
                     /locus_tag="ACUQR0_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HHV9109446.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15075..15572)
                     /locus_tag="ACUQR0_002450"
     CDS             complement(15075..15572)
                     /locus_tag="ACUQR0_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HHV9109447.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15569..15931)
                     /locus_tag="ACUQR0_002451"
     CDS             complement(15569..15931)
                     /locus_tag="ACUQR0_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HHV9109448.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15921..16268)
                     /locus_tag="ACUQR0_002452"
     CDS             complement(15921..16268)
                     /locus_tag="ACUQR0_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HHV9109449.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16377..16790
                     /gene="yidI"
                     /locus_tag="ACUQR0_002453"
     CDS             16377..16790
                     /gene="yidI"
                     /locus_tag="ACUQR0_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HHV9109450.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16837..18279)
                     /locus_tag="ACUQR0_002454"
                     /pseudo
     CDS             complement(16837..18279)
                     /locus_tag="ACUQR0_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18314..20029)
                     /locus_tag="ACUQR0_002455"
     CDS             complement(18314..20029)
                     /locus_tag="ACUQR0_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HHV9109451.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20196..21089
                     /locus_tag="ACUQR0_002456"
     CDS             20196..21089
                     /locus_tag="ACUQR0_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHV9109452.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21086..21900)
                     /locus_tag="ACUQR0_002457"
                     /pseudo
     CDS             complement(21086..21900)
                     /locus_tag="ACUQR0_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343942.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21900..>22550)
                     /locus_tag="ACUQR0_002458"
     CDS             complement(21900..>22550)
                     /locus_tag="ACUQR0_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHV9109453.1"
                     /translation="ITEPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGL
                     IDQVLPQNWIPMFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVK
                     LYSKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALHDMSQ
                     TLDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV"
BASE COUNT         5739 a         6109 c         5654 g         5048 t
ORIGIN      
        1 cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata
       61 taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga
      121 tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga
      181 aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg
      241 agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac
      301 gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt
      361 cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc
      421 agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac
      481 aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt
      541 aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
      601 aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac
      661 cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt
      721 tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc
      781 aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca gtattaacgg
      841 cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc
      901 aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca cgccgaatcc
      961 cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga
     1021 catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt
     1081 ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat
     1141 acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc
     1201 taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac
     1261 cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg acgccgacat
     1321 cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa gcaaactgaa
     1381 tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac
     1441 aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg caaacattgc
     1501 cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg
     1561 ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca
     1621 aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt
     1681 ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca
     1741 ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg tgttgcttga
     1801 gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt
     1861 atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt ttcaatgcaa
     1921 ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat
     1981 atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga cctgagcatc
     2041 ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca gcaggttctg
     2101 ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg cgttttgcag
     2161 gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg actttcccat
     2221 ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc tgagacggcg
     2281 aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag
     2341 cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg
     2401 aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga gctgcgacca
     2461 ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg atgcagtacg
     2521 acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg
     2581 taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag
     2641 tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga accatcgcca
     2701 ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca gccgttagcc
     2761 ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg
     2821 taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc ttattcatat
     2881 tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca attaatgttc
     2941 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     3001 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     3061 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     3121 aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     3181 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat
     3241 tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat ccgattccac
     3301 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
     3361 acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc
     3421 aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc cccattgccg
     3481 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
     3541 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
     3601 ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca atcaattctg
     3661 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     3721 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     3781 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     3841 acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     3901 ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat ttcatcacat
     3961 tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
     4021 tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt ccggcccaat
     4081 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata ctccggcttt
     4141 gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     4201 tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct
     4261 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc
     4321 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     4381 cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     4441 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     4501 aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc tgacgctgga
     4561 cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg cgggtattga
     4621 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
     4681 gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac cgttgatcaa
     4741 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     4801 ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     4861 gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc tgaatcacct
     4921 cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt
     4981 cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct tacaggcgga
     5041 agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg cccgccagcc
     5101 agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc gggtcattga
     5161 cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg aaaatggttt
     5221 tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac ggctggctcc
     5281 ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg cgacagtcag
     5341 ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg cgctggcggt
     5401 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aagtacaaag
     5461 tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt cgctggcgga
     5521 acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
     5581 tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg
     5641 gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaaa
     5701 gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
     5761 tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc atcaggcaga
     5821 tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccga
     5881 ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
     5941 ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
     6001 caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
     6061 gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc gtggcgataa
     6121 tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc
     6181 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
     6241 ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac
     6301 caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt
     6361 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt
     6421 cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg
     6481 gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga
     6541 agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac agcgccacac
     6601 ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg aatgtcccgc
     6661 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct
     6721 ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
     6781 ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg aacggcagga
     6841 attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc acgccataag
     6901 tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaaa
     6961 tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga
     7021 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
     7081 gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg ggttactcct
     7141 gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat
     7201 agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga
     7261 gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga
     7321 aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagcac
     7381 tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc
     7441 cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac caattaacat
     7501 ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct
     7561 ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa
     7621 attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc
     7681 accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac
     7741 gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc ggaccacata
     7801 gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catatttggt
     7861 gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc
     7921 gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca
     7981 gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc cggcacgcca
     8041 gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg
     8101 cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc aggccgttaa
     8161 cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac
     8221 ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc ccgtggcgat
     8281 aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa
     8341 cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct
     8401 gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc gcgtgaagta
     8461 aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt agcgataacg
     8521 ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg
     8581 caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac gcgtgccgtg
     8641 cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag
     8701 gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caatcaccag
     8761 catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat cggcatgttt
     8821 cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcgct
     8881 taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc cttccagctc
     8941 catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca gctggcgcgg
     9001 acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca gttgttcgat
     9061 gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa caatgcccgc
     9121 ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac gcgccacgtc
     9181 gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat tgcgcgccag
     9241 ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc ccagtaacaa
     9301 ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat cgcgccaggt
     9361 ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc cttgccagcc
     9421 atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa agcgcgacag
     9481 ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca agtaccagac
     9541 cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga gcggcagcca
     9601 ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg tcagaccgcc
     9661 agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc agggcagcga
     9721 ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc gccagtcacg
     9781 ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac taccgatcat
     9841 caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccactc
     9901 cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc ccagacgcag
     9961 accaaacgga aataacagca ccgccatatc agggcgctca accagatgca ggctgatact
    10021 ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg
    10081 ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc tacgtcgtta
    10141 ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag
    10201 cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg
    10261 cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc aatatccggc
    10321 gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc atccgggcta
    10381 cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga
    10441 ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct tagcagctcc
    10501 agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc
    10561 agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa atcaggttcc
    10621 agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg
    10681 gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg
    10741 atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg
    10801 gatcggactg attacgctgc actttcacga catcttccag cttagcgatt tggcttatca
    10861 tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg tcgctgtcct
    10921 gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa
    10981 cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg ttgttatgag
    11041 ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct ggcggcacca
    11101 tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg ccagggcgat
    11161 tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca caggtttcga
    11221 ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg gcggcaaaaa
    11281 cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct tcgttgttca
    11341 tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc tgaatattca
    11401 tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc gccagcgccg
    11461 cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag gtcagccact
    11521 ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga cccacatcgg
    11581 tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg ccgtaatggc
    11641 ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa tccgctacca
    11701 actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc gccagcacgt
    11761 catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc tctgcgcgat
    11821 cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg ccaatcgccc
    11881 ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa atatagttgg
    11941 tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct tttggcagca
    12001 tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc agttcacgca
    12061 cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt ttggcagcgt
    12121 taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca gcggcttttt
    12181 ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt
    12241 ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct
    12301 gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg atagagatgc
    12361 cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga acctgaccag
    12421 tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc accaggttag
    12481 tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg cgcgccattc
    12541 cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg atttgcgtgc
    12601 tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc gtcacaatct
    12661 taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag cgcttacgcg
    12721 tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg actttccggg
    12781 aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt
    12841 gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg accactgcgg
    12901 cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt ggggaccttg
    12961 ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga caatggaaaa
    13021 tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg cagataacaa
    13081 aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc aaagcacact
    13141 gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa
    13201 ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg tccaaatatt
    13261 actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt tatgcggggg
    13321 ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta
    13381 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
    13441 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
    13501 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
    13561 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
    13621 ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gagtcggatg
    13681 cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat tgccgctttt
    13741 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
    13801 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
    13861 ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt tattgatgtt
    13921 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
    13981 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
    14041 gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg tcggtcgccg
    14101 accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
    14161 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
    14221 cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt tgcgccatgc
    14281 taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg
    14341 tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg ttctttgtgc
    14401 tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg atgcaccgcg
    14461 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
    14521 gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct ccggcgtgct
    14581 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
    14641 tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct tctcaacgtt
    14701 gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga tccccgactg
    14761 gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc
    14821 cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct tcctggcggg
    14881 cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
    14941 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga ccttaatggg
    15001 attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc
    15061 cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
    15121 atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc ctgatagccc
    15181 gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg
    15241 cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg
    15301 gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca ctcaaacagg
    15361 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
    15421 tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg tttgaaattg
    15481 tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt atgcctgtcc
    15541 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
    15601 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
    15661 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
    15721 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
    15781 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
    15841 agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg
    15901 caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac gatcaccacc
    15961 acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag gtcctctttc
    16021 agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc taacccgccg
    16081 gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc gaaatctggc
    16141 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg gatccacgcc
    16201 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa
    16261 attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg ttttttttaa
    16321 gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga ggtcagatgg
    16381 gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc atcgctttaa
    16441 tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca acgcttgaaa
    16501 gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga ataaaaggcg
    16561 aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc ctcgatcaaa
    16621 gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt atgcgtaagg
    16681 aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt
    16741 tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa gcagcctgaa
    16801 taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt ttttctcctc
    16861 caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc
    16921 atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt tacgccacgg
    16981 acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa
    17041 ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat
    17101 ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag
    17161 tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc
    17221 aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg caaggatatt
    17281 ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa
    17341 atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat
    17401 cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc
    17461 gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac gttgctctgg
    17521 cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc aggcaaaata
    17581 gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa
    17641 gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta
    17701 aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc
    17761 atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca gaaaatctac
    17821 cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa
    17881 atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct
    17941 aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac actcgccagt
    18001 gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc
    18061 atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca
    18121 cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag gcgtacaaac
    18181 cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt
    18241 ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt
    18301 caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt
    18361 gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg atgaaataac
    18421 tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg acatagacgc
    18481 caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac ggtttcatgt
    18541 ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca
    18601 ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt
    18661 cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt
    18721 ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg
    18781 gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttgg
    18841 cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat ttgcgcccga
    18901 cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac
    18961 tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc gcaccaaata
    19021 acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc agcgtcgggt
    19081 aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac cctggcagca
    19141 ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt
    19201 ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa
    19261 aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc
    19321 caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg ccctgcataa
    19381 agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca
    19441 gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat
    19501 acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa
    19561 tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa
    19621 caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg
    19681 tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat
    19741 agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc
    19801 ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt tgttccgtgg
    19861 aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa
    19921 gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag gtgatcaccg
    19981 ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct
    20041 tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg
    20101 cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc
    20161 aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag
    20221 atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca
    20281 gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct
    20341 ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac
    20401 aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat agcaactgta
    20461 ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac
    20521 agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt cagcaaagcg
    20581 ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt
    20641 caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt
    20701 ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat
    20761 taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca
    20821 cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc
    20881 cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag
    20941 gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca
    21001 aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
    21061 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
    21121 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg
    21181 taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
    21241 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
    21301 gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg
    21361 cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg
    21421 gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcta
    21481 ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg
    21541 catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag
    21601 tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac
    21661 aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg caatgtccgg
    21721 cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc
    21781 gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa
    21841 cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat
    21901 tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg
    21961 agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc atcgccacta
    22021 cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg
    22081 tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat attgccgacc
    22141 ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgcagcg
    22201 gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct
    22261 tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac cacgaagtac
    22321 agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg
    22381 aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc
    22441 accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca
    22501 aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
//
LOCUS       DBKELA010000063        22159 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.063, whole genome shotgun sequence.
ACCESSION   DBKELA010000063 DBKELA010000000
VERSION     DBKELA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.063"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="ACUQR0_002459"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="ACUQR0_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HHV9109454.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="ACUQR0_002460"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="ACUQR0_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HHV9109455.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="ACUQR0_002461"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="ACUQR0_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HHV9109456.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="ACUQR0_002462"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="ACUQR0_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HHV9109457.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="ACUQR0_002463"
     CDS             3251..5011
                     /locus_tag="ACUQR0_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHV9109458.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="ACUQR0_002464"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="ACUQR0_002464"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HHV9109459.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="ACUQR0_002465"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="ACUQR0_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HHV9109460.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="ACUQR0_002466"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="ACUQR0_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HHV9109461.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="ACUQR0_002467"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="ACUQR0_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HHV9109462.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="ACUQR0_002468"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="ACUQR0_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HHV9109463.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="ACUQR0_002469"
     CDS             complement(9679..11586)
                     /locus_tag="ACUQR0_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9109464.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="ACUQR0_002470"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="ACUQR0_002470"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HHV9109465.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="ACUQR0_002471"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="ACUQR0_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HHV9109466.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="ACUQR0_002472"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="ACUQR0_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HHV9109467.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="ACUQR0_002473"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="ACUQR0_002473"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HHV9109468.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="ACUQR0_002474"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="ACUQR0_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="ACUQR0_002475"
     CDS             complement(17598..18113)
                     /locus_tag="ACUQR0_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9109469.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="ACUQR0_002476"
     CDS             complement(18121..18663)
                     /locus_tag="ACUQR0_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9109470.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18675..19744)
                     /locus_tag="ACUQR0_002477"
                     /pseudo
     CDS             complement(18675..19744)
                     /locus_tag="ACUQR0_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(19735..22113)
                     /locus_tag="ACUQR0_002478"
                     /pseudo
     CDS             complement(19735..22113)
                     /locus_tag="ACUQR0_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT         5493 a         5803 c         5205 g         5658 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaatggt aatgactcca acttactgat agtgttttat
    22141 gttcagataa tgcccgatg
//
LOCUS       DBKELA010000064        21502 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.064, whole genome shotgun sequence.
ACCESSION   DBKELA010000064 DBKELA010000000
VERSION     DBKELA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.064"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACUQR0_002479"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACUQR0_002479"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HHV9109471.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACUQR0_002480"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACUQR0_002480"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HHV9109472.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVGF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACUQR0_002481"
     CDS             complement(3121..3420)
                     /locus_tag="ACUQR0_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HHV9109473.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACUQR0_002482"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACUQR0_002482"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HHV9109474.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACUQR0_002483"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACUQR0_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HHV9109475.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACUQR0_002484"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACUQR0_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HHV9109476.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACUQR0_002485"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACUQR0_002485"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HHV9109477.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACUQR0_002486"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACUQR0_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACUQR0_002487"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACUQR0_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9109478.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACUQR0_002488"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACUQR0_002488"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HHV9109479.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACUQR0_002489"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACUQR0_002489"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HHV9109480.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13864
                     /gene="xylR"
                     /locus_tag="ACUQR0_002490"
                     /pseudo
     CDS             12687..13864
                     /gene="xylR"
                     /locus_tag="ACUQR0_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; frameshifted;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13972..14796)
                     /locus_tag="ACUQR0_002491"
     CDS             complement(13972..14796)
                     /locus_tag="ACUQR0_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HHV9109481.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="ACUQR0_002492"
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="ACUQR0_002492"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HHV9109482.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="ACUQR0_002493"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="ACUQR0_002493"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HHV9109483.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="ACUQR0_002494"
     CDS             complement(18728..19201)
                     /locus_tag="ACUQR0_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HHV9109484.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="ACUQR0_002495"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="ACUQR0_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HHV9109485.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="ACUQR0_002496"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="ACUQR0_002496"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HHV9109486.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21502
                     /locus_tag="ACUQR0_002497"
     CDS             21362..>21502
                     /locus_tag="ACUQR0_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HHV9109487.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5398 a         5488 c         5282 g         5334 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagccca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaactggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gtcttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgggcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcgcccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatata catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acgcctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctgg ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag ggcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgttgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgctcag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagttccgc atattgaaat tttaagccaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cg
//
LOCUS       DBKELA010000065        21377 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.065, whole genome shotgun sequence.
ACCESSION   DBKELA010000065 DBKELA010000000
VERSION     DBKELA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.065"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..124)
                     /locus_tag="ACUQR0_002498"
     CDS             complement(<1..124)
                     /locus_tag="ACUQR0_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109488.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLT"
     gene            complement(226..1206)
                     /locus_tag="ACUQR0_002499"
     CDS             complement(226..1206)
                     /locus_tag="ACUQR0_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHV9109489.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1214..1924)
                     /locus_tag="ACUQR0_002500"
     CDS             complement(1214..1924)
                     /locus_tag="ACUQR0_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHV9109490.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2141..3964)
                     /gene="typA"
                     /locus_tag="ACUQR0_002501"
     CDS             complement(2141..3964)
                     /gene="typA"
                     /locus_tag="ACUQR0_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HHV9109491.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4337..5746
                     /gene="glnA"
                     /locus_tag="ACUQR0_002502"
     CDS             4337..5746
                     /gene="glnA"
                     /locus_tag="ACUQR0_002502"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HHV9109492.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5919..6968
                     /gene="glnL"
                     /locus_tag="ACUQR0_002503"
     CDS             5919..6968
                     /gene="glnL"
                     /locus_tag="ACUQR0_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HHV9109493.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6980..8389
                     /gene="glnG"
                     /locus_tag="ACUQR0_002504"
     CDS             6980..8389
                     /gene="glnG"
                     /locus_tag="ACUQR0_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HHV9109494.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8501..8611
                     /locus_tag="ACUQR0_002505"
     CDS             8501..8611
                     /locus_tag="ACUQR0_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HHV9109495.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8840..10213)
                     /gene="hemN"
                     /locus_tag="ACUQR0_002506"
     CDS             complement(8840..10213)
                     /gene="hemN"
                     /locus_tag="ACUQR0_002506"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HHV9109496.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10402..10911)
                     /gene="yihI"
                     /locus_tag="ACUQR0_002507"
     CDS             complement(10402..10911)
                     /gene="yihI"
                     /locus_tag="ACUQR0_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HHV9109497.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11030..11196)
                     /locus_tag="ACUQR0_002508"
                     /pseudo
     CDS             complement(11030..11196)
                     /locus_tag="ACUQR0_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11493..12125
                     /gene="yihA"
                     /locus_tag="ACUQR0_002509"
     CDS             11493..12125
                     /gene="yihA"
                     /locus_tag="ACUQR0_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HHV9109498.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12507..15293)
                     /gene="polA"
                     /locus_tag="ACUQR0_002510"
     CDS             complement(12507..15293)
                     /gene="polA"
                     /locus_tag="ACUQR0_002510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HHV9109499.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHWATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15657..16589
                     /locus_tag="ACUQR0_002511"
     CDS             15657..16589
                     /locus_tag="ACUQR0_002511"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HHV9109500.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16630..18060)
                     /locus_tag="ACUQR0_002512"
     CDS             complement(16630..18060)
                     /locus_tag="ACUQR0_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HHV9109501.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18215..18841)
                     /gene="dsbA"
                     /locus_tag="ACUQR0_002513"
     CDS             complement(18215..18841)
                     /gene="dsbA"
                     /locus_tag="ACUQR0_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HHV9109502.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18858..19844)
                     /gene="srkA"
                     /locus_tag="ACUQR0_002514"
     CDS             complement(18858..19844)
                     /gene="srkA"
                     /locus_tag="ACUQR0_002514"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HHV9109503.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19921..20190)
                     /locus_tag="ACUQR0_002515"
     CDS             complement(19921..20190)
                     /locus_tag="ACUQR0_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HHV9109504.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20260..20844
                     /gene="mobA"
                     /locus_tag="ACUQR0_002516"
     CDS             20260..20844
                     /gene="mobA"
                     /locus_tag="ACUQR0_002516"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HHV9109505.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20826..21353
                     /gene="mobB"
                     /locus_tag="ACUQR0_002517"
     CDS             20826..21353
                     /gene="mobB"
                     /locus_tag="ACUQR0_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HHV9109506.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5231 a         5363 c         5141 g         5642 t
ORIGIN      
        1 tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc gacggtaatt
       61 tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc tttttcgtga
      121 gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa gataccgacc
      181 attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt ggttcagaag
      241 atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta tctgattaat
      301 tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg gtaggggtgt
      361 ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa agcggaacgc
      421 cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg gctcatcatc
      481 gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct gacctgaaat
      541 acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt taatatggac
      601 ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat ttgcattgat
      661 catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt tgaccagcga
      721 caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca ggccgctgtc
      781 ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata cgtcgcgcaa
      841 attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca acgcgatggc
      901 gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa tatcaaggtt
      961 cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat taccaagaga
     1021 aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt cacttgcgat
     1081 atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa aggcataagc
     1141 atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt tatttattgt
     1201 caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc caatattcaa
     1261 tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt aagatcgctg
     1321 gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg acgggtttga
     1381 aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact gcagaaaatg
     1441 agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata tatgtttcgt
     1501 gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag agttcagttg
     1561 atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta cggtttttct
     1621 cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga aagctcgccg
     1681 acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta cgatccttac
     1741 ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc atacgtttaa
     1801 tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc gtggtgatgc
     1861 catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat tgattctccg
     1921 ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac gccaaaaaag
     1981 aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct attgacgtaa
     2041 tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt aaaaaaaaac
     2101 ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct ttcggtgcgc
     2161 ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggtcg
     2221 gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc
     2281 ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg ttggtcagtt
     2341 tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata ccgataatct
     2401 gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga
     2461 acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg ttctgacgct
     2521 gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagaccag
     2581 taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca
     2641 cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac
     2701 gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc agcgtcacgt
     2761 tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc ggacgggata
     2821 ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg tgcagttcac
     2881 cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc agcgcaacgt
     2941 tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg aacttacctt
     3001 ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa
     3061 cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca
     3121 gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg gtttcgatac
     3181 gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt ttgccttcgc
     3241 tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg cggccaatgc
     3301 cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt
     3361 caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc ggggtcatgt
     3421 cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga
     3481 tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat
     3541 ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct
     3601 tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa
     3661 atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca acttcaccac
     3721 cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag
     3781 cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca
     3841 cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta
     3901 ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga
     3961 tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta
     4021 caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct
     4081 ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg cttaatgttt
     4141 ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt tgcacgatgt
     4201 taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt
     4261 ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac aatgaccaat
     4321 ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt
     4381 gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg tcactatccc
     4441 tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg gctcctcgat
     4501 tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg catccactgc
     4561 agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca tccttgaacc
     4621 tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg ccgaagatta
     4681 cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct
     4741 gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta tcgacgatat
     4801 cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc accgtccggc
     4861 agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata ttcgttctga
     4921 aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc acgaagtagc
     4981 gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga
     5041 aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta aaaccgcgac
     5101 ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc acatgtctct
     5161 gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt ctgagcaggc
     5221 gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc tggcaaaccc
     5281 gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa tgctggctta
     5341 ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg
     5401 tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct ttgctgccct
     5461 gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag ccatggacaa
     5521 aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg caggctctct
     5581 ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg gtggcgtgtt
     5641 cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg
     5701 tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta
     5761 ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc atctctgcat
     5821 gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa aaagctataa
     5881 tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc acgcagcccg
     5941 atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat gacaatctgg
     6001 cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc cgcaaattgt
     6061 ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag ctgatgcaag
     6121 aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc atcgatggtc
     6181 gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc ctgctggaga
     6241 tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat gctcaacaag
     6301 tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg cttggcggtt
     6361 tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta ctcgaatata
     6421 ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt ctgttggggc
     6481 cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa cgcgtggtaa
     6541 cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac gatcccagcc
     6601 tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat attgtgcgca
     6661 atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc cgcaccgcgt
     6721 ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat gtggaagata
     6781 acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg gtcagcggcc
     6841 gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat cagcattcag
     6901 gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac ctgcctatca
     6961 ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg acgatagttc
     7021 catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta cgacatttga
     7081 gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc tgctttcaga
     7141 tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac agcgccatcc
     7201 aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg tcagcgccta
     7261 tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag cagtggcgct
     7321 ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata ttcagcttaa
     7381 cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt tccgtattat
     7441 cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg gcaccggtaa
     7501 agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc cgtttatcgc
     7561 gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg gccacgagaa
     7621 aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg ccgatggcgg
     7681 cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc gtttgctgcg
     7741 cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga aagtggatgt
     7801 gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag gtaagttccg
     7861 tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc tgcgcgaacg
     7921 tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc gcgaactggg
     7981 cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc tggcgtggcc
     8041 aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg ccgccgggca
     8101 ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg cggagagtac
     8161 ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag acagagcgct
     8221 gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc ggacgttact
     8281 gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc tactcggctg
     8341 gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat tcacggcttg
     8401 tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac tgttccgata
     8461 agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat caataattaa
     8521 tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg ctgccgtgct
     8581 gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg atgcggagaa
     8641 agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc agatgccaga
     8701 tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt tttgttgccc
     8761 agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa
     8821 acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc gctttctggc
     8881 gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca
     8941 cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct
     9001 tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca agaggggcgt
     9061 aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac
     9121 gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc tgatagtact
     9181 gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc
     9241 ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta cgatgcagca
     9301 cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga tccataccga
     9361 taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt
     9421 gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca
     9481 gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca cgtttcaggg
     9541 taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg
     9601 aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct tcatcctgct
     9661 cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg ctcaggcgat
     9721 taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt
     9781 cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc ttcatcagac
     9841 ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc aattggctga
     9901 cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat
     9961 actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa
    10021 gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca
    10081 cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg
    10141 aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt
    10201 gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg
    10261 atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg
    10321 tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg
    10381 aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt
    10441 ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc
    10501 atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca gggtttcgcc
    10561 tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc
    10621 cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc
    10681 agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg gtgcgttctg
    10741 gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg
    10801 acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc gtgtttttcg
    10861 acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg
    10921 tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag
    10981 aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct
    11041 tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac aatccgtgtt
    11101 gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt
    11161 cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct
    11221 atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac
    11281 attttcagag ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc
    11341 gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg
    11401 cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg
    11461 ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc
    11521 attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg
    11581 cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga
    11641 aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag
    11701 tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag
    11761 agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc
    11821 aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga
    11881 ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa gcggacaaac
    11941 tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta ctggcgttta
    12001 acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta gacaagctga
    12061 ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg
    12121 aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat
    12181 agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac ggatcttttc
    12241 tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca
    12301 aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg
    12361 cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcagt
    12421 tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac
    12481 gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc
    12541 acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc
    12601 ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc gtgtacctgc
    12661 atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc
    12721 cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc
    12781 gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc
    12841 agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat atactccagc
    12901 acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg
    12961 ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct
    13021 ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa cacttctgcc
    13081 gccgttgccc agtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttatcacgc
    13141 gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa
    13201 tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg caccggaatg
    13261 ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa
    13321 gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaagtc
    13381 gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc
    13441 gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc
    13501 ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga agaaaggtta
    13561 aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg caaggtgagc
    13621 tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt
    13681 gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt
    13741 ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc atcttcggcg
    13801 gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt tttgccttta
    13861 cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc
    13921 atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca
    13981 atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc
    14041 ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg
    14101 tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata tgccgctacg
    14161 cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg
    14221 gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt
    14281 gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct
    14341 ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc ccctttggcc
    14401 tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac
    14461 agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc
    14521 agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac ttctttgttt
    14581 tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct
    14641 ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg cgcggttttt
    14701 tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag cgccaggaag
    14761 tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg
    14821 gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta
    14881 tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg
    14941 ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc
    15001 atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg gcgatgtgat
    15061 ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct
    15121 gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc
    15181 gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa
    15241 agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc
    15301 gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt
    15361 tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa tcgtcatgtg
    15421 gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc
    15481 atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa taattgagat
    15541 aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt
    15601 tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca ctgtctatgg
    15661 ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga
    15721 gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc attgccggga
    15781 ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt
    15841 ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc
    15901 aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta
    15961 accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga
    16021 tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc ctggcgtgct
    16081 gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccgg
    16141 agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc
    16201 ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa
    16261 cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa
    16321 atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata
    16381 accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc gtacatgtgg
    16441 atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa agctttaagc
    16501 gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt ctgacgtctc
    16561 tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga gcagaggcga
    16621 gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatctc
    16681 ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg cggtattgtt
    16741 gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga tttgtttatc
    16801 agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct gttttgcggt
    16861 ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga tatctttgtt
    16921 tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc
    16981 gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt tttgtaataa
    17041 cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc
    17101 taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga tgataaattg
    17161 gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg
    17221 ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga tataatcttt
    17281 gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg caacgtcttc
    17341 cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt
    17401 cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat
    17461 tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga
    17521 actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc catactctgc
    17581 aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc
    17641 cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa
    17701 gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca ttgtgatggg
    17761 caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct tgatattgag
    17821 atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa aaaagccttt
    17881 gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct ttttcgcatc
    17941 gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg tgtaccatgt
    18001 gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat
    18061 ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg aaataatgtc
    18121 cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa aaaagcccgt
    18181 gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac agatatttca
    18241 ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata ccctgcggat
    18301 tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc acatcagctg
    18361 cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac gccgcgtcgt
    18421 actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat gctgaacgaa
    18481 tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct tccacgccca
    18541 gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca cccatgaagt
    18601 tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca ttatcagaaa
    18661 tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa aactccagca
    18721 cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca tcttcatact
    18781 gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa atctttttca
    18841 tgatctactc tctccgatta atacataggt gttaattgca aagggggttc ttgtagaact
    18901 tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc ggttaaccac
    18961 gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc aagataataa
    19021 accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc gaactcgcta
    19081 aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc tttatcgcca
    19141 ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc cagatcaaca
    19201 aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg tagccgcaga
    19261 acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc tgtcgctttc
    19321 agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag cttgcgaggc
    19381 tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg tttgcgcccc
    19441 gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat attatcagct
    19501 tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg atgattcaat
    19561 aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt taccagctgc
    19621 aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg gcgataaaat
    19681 ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt ttcatagctg
    19741 ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc gtccatgatg
    19801 gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc atccggaaaa
    19861 cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc ttacaagctt
    19921 ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt cttcataatc
    19981 tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca ggtgatagat
    20041 cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt tcgccagttt
    20101 ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct ggaggagttc
    20161 aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg agacagacac
    20221 gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat gacgacgata
    20281 acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga taaaggattg
    20341 ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa gacgcagctc
    20401 tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg cggtctgaaa
    20461 gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct ttcagtaatg
    20521 cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta cattccccat
    20581 gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg ggtccatgac
    20641 ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc tttattactg
    20701 gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc tggcggtcat
    20761 gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc agaggagctt
    20821 gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg agtggtaccg
    20881 gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg atccgtccgg
    20941 ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa gatagctatg
    21001 agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga tgggccttga
    21061 tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt cgaatggata
    21121 cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc gcaaagattg
    21181 tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac aggcatgtta
    21241 ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat attaatgatg
    21301 ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga taagcaagag
    21361 taggctggat cagacgc
//
LOCUS       DBKELA010000066        21301 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.066, whole genome shotgun sequence.
ACCESSION   DBKELA010000066 DBKELA010000000
VERSION     DBKELA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.066"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(315..533)
                     /gene="infA"
                     /locus_tag="ACUQR0_002518"
     CDS             complement(315..533)
                     /gene="infA"
                     /locus_tag="ACUQR0_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HHV9109507.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(818..1522)
                     /gene="aat"
                     /locus_tag="ACUQR0_002519"
     CDS             complement(818..1522)
                     /gene="aat"
                     /locus_tag="ACUQR0_002519"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HHV9109508.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1564..3285)
                     /gene="cydC"
                     /locus_tag="ACUQR0_002520"
     CDS             complement(1564..3285)
                     /gene="cydC"
                     /locus_tag="ACUQR0_002520"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HHV9109509.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3286..5052)
                     /gene="cydD"
                     /locus_tag="ACUQR0_002521"
     CDS             complement(3286..5052)
                     /gene="cydD"
                     /locus_tag="ACUQR0_002521"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease/ATP-binding
                     protein CydD"
                     /protein_id="HHV9109510.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5175..6140)
                     /gene="trxB"
                     /locus_tag="ACUQR0_002522"
     CDS             complement(5175..6140)
                     /gene="trxB"
                     /locus_tag="ACUQR0_002522"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HHV9109511.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6684..7178
                     /gene="lrp"
                     /locus_tag="ACUQR0_002523"
     CDS             6684..7178
                     /gene="lrp"
                     /locus_tag="ACUQR0_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HHV9109512.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7313..11341
                     /gene="ftsK"
                     /locus_tag="ACUQR0_002524"
     CDS             7313..11341
                     /gene="ftsK"
                     /locus_tag="ACUQR0_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HHV9109513.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            11496..12107
                     /gene="lolA"
                     /locus_tag="ACUQR0_002525"
     CDS             11496..12107
                     /gene="lolA"
                     /locus_tag="ACUQR0_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HHV9109514.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12118..13461
                     /gene="rarA"
                     /locus_tag="ACUQR0_002526"
                     /gene_synonym="mgsA"
     CDS             12118..13461
                     /gene="rarA"
                     /locus_tag="ACUQR0_002526"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HHV9109515.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13552..14844
                     /gene="serS"
                     /locus_tag="ACUQR0_002527"
     CDS             13552..14844
                     /gene="serS"
                     /locus_tag="ACUQR0_002527"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HHV9109516.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15084..17528
                     /gene="dmsA"
                     /locus_tag="ACUQR0_002528"
                     /pseudo
     CDS             15084..17528
                     /gene="dmsA"
                     /locus_tag="ACUQR0_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            17539..18156
                     /gene="dmsB"
                     /locus_tag="ACUQR0_002529"
     CDS             17539..18156
                     /gene="dmsB"
                     /locus_tag="ACUQR0_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HHV9109517.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18158..18949
                     /locus_tag="ACUQR0_002530"
     CDS             18158..18949
                     /locus_tag="ACUQR0_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HHV9109518.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(18985..19611)
                     /gene="ycaC"
                     /locus_tag="ACUQR0_002531"
     CDS             complement(18985..19611)
                     /gene="ycaC"
                     /locus_tag="ACUQR0_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HHV9109519.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19926..21074
                     /locus_tag="ACUQR0_002532"
     CDS             19926..21074
                     /locus_tag="ACUQR0_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109520.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21092..>21301
                     /locus_tag="ACUQR0_002533"
     CDS             21092..>21301
                     /locus_tag="ACUQR0_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9109521.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         5263 a         5342 c         5625 g         5071 t
ORIGIN      
        1 cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac
       61 caaattgttt tgctaccaaa cctcatgggt ggcaacgggg cgctactata gggagttgga
      121 gtaaaacggt caagaagaat tttaatgata attattgttt gctcatactg taaacaactt
      181 gtgcagtata tctacatcga gacaagttat ggacttatac ttccaaagta cttcatacat
      241 atcacaaaat aaaaaggccg gttaaaccga ccttttactc gttctttctc ttcgcccatc
      301 aggcggtaaa acaatcagcg actacggaag acaatgcggc ctttgctcag gtcgtacggg
      361 gtcagttcaa cagtcacttt gtcgcccgtc aggatgcgga tgtagttttt gcgcatttta
      421 ccggagatgt gtgcagtaac cacgtgaccg ttttctaact ctacgcggaa catggtatta
      481 ggcaacgttt caagaacggt accttgcatt tcaatattgt cttctttggc catctaatcc
      541 tctggggtat cactaccgta atttgaaccg gcaagataat gccgaagttc tgtaaataag
      601 taaagatttg cgcgctaaat cgcaacaaac aggttcggca cattactccg aaaacacacg
      661 gctaagccgc accaaaagcg caacgtataa gggagcggtg agataaacga tgggcgttac
      721 ctgacgcgaa aaattcctta tcggcagcgg ggtaatgagc gtaaccaact ctgcgaccgc
      781 aattataaca ctctggggag aaatgtgccg aaaacattca ttcttgtggt gaaaacaagc
      841 accgtggtac ccagaaatta ttcggcaatc gtccgaggcg catttgattg agataattaa
      901 ggtaatcccg gcggggaatt tcgcaggcac caagcgatgc tgtgtgatcg ttaaggacct
      961 ggcagtcgat aagcttaccg ccatgaccga taaattcctc acagaatacc agaagcgccg
     1021 ttttagacgc attttccatc cggctgaaca tggactcgcc acaaaatagc gttccctggg
     1081 ccacgccgta cataccgccg acaagctcat cttcacgcca gacttcaatg gagtgggcat
     1141 gaccgagttc gtgaaggcga tggtaggctt cgaccacgcc acgcgtgatc caggttcctt
     1201 cttcgcgatc gctggcacag ccttcaatga cctgaccaaa agcgtaattc atcgtgacac
     1261 gatagggcga gcgtttatga aatcgcttca tactacggct gatatgcagt gattctggcc
     1321 atagcaccgc gcggggatcg ggcgaccacc agaggatggg gtcgcctgga gaaaaccacg
     1381 gaaaaatacc acgctggtaa gccattaaca ggcgcgcagg gctaagatcg cccccaagtg
     1441 ccagcaggcc gttaggctca cgtaatgcgc cttccgggga agggaaggct attgaatggc
     1501 gagaaagctg aaccaagcgc atgaccgcaa aactccacgc aagtcggatc gttcaataat
     1561 agcttacaaa ccctgcttga actggtaata acgcccctgt ctggcaagca gttctgcgtg
     1621 agtaccttgc tcaataattt gcccgttgtc catcactatt atttgttgga aacgagagag
     1681 tccgcgaagt cgatgggtga ccattaacac cgttttctca cgcatcattt ttgcaagcaa
     1741 ttcaaggatc tggctttcgg ttgtggcatc taagccttcg gtaggttcat ccagcaacac
     1801 cagtggcgca tcatgtaaca gcgtacgggc gatagccaga cggcgcagtt caccaccgga
     1861 gagctggcgt ccgccttcac ctaaccaact gttgagacct gcatcctcga gcagcttctc
     1921 cagaccaacg cgacgcaaga tctccgacag agcctcatca ctactgccag gcgaggcgag
     1981 taacagatta tcacgcagcg tggcgctaaa cagatgcact cgctgaggaa caacgctgat
     2041 ggtctgtcgt agagccgctt cattcaggct ggctatgggg ctatcgttaa gcaaaatctc
     2101 gccctgttgc gggtcccatg cgcgggtcag cagttgtaac agtgttgatt tgccgcatcc
     2161 ggttcgcccg agaatcgcta tatgttcccc ggcgtttacc tgaagagaaa tccctttaag
     2221 tgcctgttgg gattgctccg gataagtgaa ctgaacatcc cgtaatgtca gcgaaacgcg
     2281 atcggcaaca cgagtttggg tatcagggaa ggtgacctcc ggtttttgat ccgttaagtc
     2341 agagatacgt acggcagagg caatgacttg ccccagatgc tgaaatgcac ccgttactgg
     2401 tgccagtgct tcaaacgcgg ctaacgcaca aaagacaaac agggcaatta atgcgccggg
     2461 ttgagcattg ccgccaacgc cgccagacgc catccacagc atcaggatca ccgctaacgc
     2521 gccaatgagc agcattatcg cttgcgacaa tgcggtcagt tcagattgac ggcgttgcgc
     2581 ttccagccat tgaatttctg tattctctag ttgcgtgcga taacgatcgc tggcaccaaa
     2641 aatggtcagc tcagcttgcc cttgcagcca ggccgtcagt tgttggcgat actgtccgcg
     2701 aagatgagtc agattttgcc cggtgctttt tcccgcacga taaaacagcg gtggcatcag
     2761 gaaaagcgtc agtaacataa cgctgcccag cgtaaaggcg agggtgaaat caaggaaact
     2821 taacccgatt gtcaccacca taatcaccac aaaagcgccc accagcggcg agataacgcg
     2881 caggtaaaga tgatcgagcg tatcaacatc cgccaccacg cggttgagca attcgccctg
     2941 acgatagcgc gccagtccgg caggggagag gggcaacaat ttgctgaagg tgtaaatgcg
     3001 cagatgctgc aacacgcgga aagtcgcgtc gtgacttacc agacgttcaa aataacgccc
     3061 ggcagtacgg gtgattgcgg caccacgcac gcccgcagcg ggtagcatat agttgaagct
     3121 gtacagtccg gcaacccccg caaccgctga ggccgagagg aaccagccgg aaagtgtcaa
     3181 cagaccgata ctggcgagca gcgtcacaat tgccagcaca ataccaagac ttaacatcca
     3241 tttatgacgt ttatacagtg ccagataggg tagcaaagcg cgcatttaaa tctcctcctg
     3301 acgatgggcc agtaatgtgg cgaatgggcc accagccaca cttaattccg cgtaacgtcc
     3361 ttgctcaata atccggccat cctgcataac ccaaatgaca tcccagtcag caagatcttc
     3421 taactggtgg gtgaccatta acgttgtctg gcgcagagag gcggcattca gcgcctccat
     3481 tacgcgctgt tcactgtgag catcaaggct ggcagcgggt tcatccaaca gtaatagcga
     3541 acagggattt agtaacgcac gggccaccgc cacgcgctgc gcctgcccca cggaaaggcg
     3601 ggcagcctgg tcgccaacag gtgtatcaac gccttgtggc aggagcggta gaaactcgct
     3661 gacccaggcg ttatccagcg ctgcttgtaa ttcttgttcg ctggcatcag gtcgcgccag
     3721 tagtacgtta tcccgcaatt ttgctgccgg taattgtggg ttttgcccaa cccaggagag
     3781 atgtttacgc catgattctg gtgataaatc acgtaattct atcccgttga ttcgtaatga
     3841 tccctgatat gagagaaaac cagaaagcgc gttcagcagt gagcttttac ctgaaccgct
     3901 gcggccaacc aacactgcac gttggcctgc tggcaaagta aagttcagcg gtccggccag
     3961 cgttttacct tccggcgacg tgataaacag atcctcggcc tcaatggtca gcggatcggt
     4021 caatgctaat tccgcctcac cgcgctgcgg atgggcgagc ggggtttcca taaacgtttt
     4081 cagactgtca gctgcaccaa cagcctgggc tttagcatga taaaacgtac cgagatcgcg
     4141 taatggctgg aaaaactctg gcgcaaggat cagggccaga aaacctgcag ccagcgtcac
     4201 cccagtatcg tagtgaccaa aatccagctc gccgagatag gaaaaaccaa agtagaccgc
     4261 caccagagca attgacagcg aggtaaaaaa ttcgagaatg ccggaggata aaaacgccag
     4321 tcgtagcact tccattgtcc gttggcggaa atcttccgaa gcagaacgaa tactttcaat
     4381 ttcagcttca ccacgaccaa aaatacgcaa tgtttccatg ccgcgcaggc gatcgaggaa
     4441 atgcccactt aagcgagcaa gagcgagaaa gttacgccgg ttagcatcgg cagcccccat
     4501 tccaaccagc gccataaaca acggaattag cggtgctgtg cccagcagaa tgagcgccgc
     4561 agcccagtta gaggggaaga tcgccaccac aatcagcaac ggcaccgaca ctgccagcgc
     4621 catttgcggc agatagcgtg catagtaatc atgcatatcg tcaatttgct cgagtaccag
     4681 cgtcgcccag ctccccgcag gtttaccctg aatccacgct ggccctgctt gttgcagacg
     4741 gtcgagaacc tgacggcgga tggcaaagcg gatatgctgc ccggcgtgat aacccacccg
     4801 ttcgcgtaac cagaccaccc atgcgcgcaa tacaaaggtc agaaccagta acgtaaaggg
     4861 aagcagcagg gcttcacggg gaatattctc cataatcata tgttgcagaa ttcgcgccat
     4921 gaaccaggcc tgggcaatga tcaatatgcc gcttacaaag cccagcagac gagaaatatt
     4981 cagccaacgt tgggagatga cgctttgctg ttttaaccag cgggttaact ctttttgacg
     5041 agatttattc attgcacgct tagcaggtga gttatcagaa ttatttgcag agcaatgtta
     5101 caacggggaa aaaataaagg cgacccatag tcgcaaggtg tcgccttctt tacttttgtt
     5161 actgatttgt aaaattattt tgcgtcagct aaaccatcga ggtagcgttc cgcatcaagt
     5221 gctgccatgc agccagtacc ggccgaagta atggcctggc gataaatgtg atccatcacg
     5281 tcgcctgcgg caaagacgcc aggaatgctg gtctgggtgg cattaccatg aatacccgac
     5341 tgtactttga tgtagccgtt ttccagttcc agctgccctt cgaaaatcgc agtattcggg
     5401 ctgtgaccga tagcaacaaa cagaccggca acgtcgagtg actcgatgtt atcgctgttt
     5461 tgcgtatcgc gcagacgaac gccagtgaca cccatttgat cgccggtcac ttcttccagc
     5521 gtacggttgg tgtgcagaat gatgttgccg ttctccactt tatccatcag acgcttaata
     5581 aggatttttt ccgcgcggaa accgtcacgg cggtgaatca gatgcacttc cgaagcgatg
     5641 ttagacagat acagcgcctc ttcaaccgcg gtattgccgc cgccgatgac cgcaactttc
     5701 tggttgcgat agaagaaacc gtcgcaggtt gcacaagcag aaaccccacg gcctttaaag
     5761 gcttcttcag agggcaggcc gagatagcgt gcagaagctc cggtggcaat aatcagcgcg
     5821 tcgcaagtgt attcggcgtt atcgccattc agacggaacg gacggttttg cagatccacc
     5881 ttgttgatat gatcaaaaat gatctcagtt tcaaacttgg tggcatgttc gtgcatgcgc
     5941 gccattaata acggaccggt cagatcgttt ggatcaccag gccagttttc cacttccgtg
     6001 gtggtggtca gttggccgcc tttttccatg ccggtaatca gcacaggttg caggttggcg
     6061 cgcgccgcgt agacagcagc ggtgtatccc gccgggcctg aacccaggat aagcagttta
     6121 ctgtgtttgg tcgtgcccat gagatcccca tagttgttgg cagacaatgg gcaggattgt
     6181 agggaattta cagacgtaaa aaaagagtat gacgattttg ttaacaattt gtgcaatcgg
     6241 cagcatcgat aagcaggtca aattctcccg tcattatcac ctctgctact taaatttccc
     6301 gctttataag ccgattaaat gatgaataaa cgcccttgtt aatgaatatc tggcatgttg
     6361 tactaaaaat cgatgttttg ctttgacaat cccctagtgt tttgcgaaaa cattcgagga
     6421 agaaaaaaac agtattctta tatgcgcata accatgtatg taaataccat gtttaccgtg
     6481 ctagtgaaat ctacgtatgg cgtggacaga cgccattcgt gatgtcgata gctgccacaa
     6541 ggcaacggtc ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc atgatttcgg
     6601 tctatcgtga cgggtagcga ctctgaacag tgatgtttca gggtcagaca ggagtaggga
     6661 aggaatacag agagacaata ataatggtag atagcaagaa gcgccctggc aaagatctcg
     6721 accgtatcga tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg
     6781 agctttctaa acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg
     6841 aaagacaagg gtttattcag ggctatacgg cgctgcttaa cccccattat ctggatgcat
     6901 cacttctggt attcgttgag attactctga atcgtggcgc accggatgtg tttgaacaat
     6961 tcaataccgc tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta tctggtgatt
     7021 tcgactacct gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg
     7081 aaaccctgct gcgtctgcct ggcgtcaatg acacacggac atacgttgtt atggaagaag
     7141 tcaagcagag taatcgtctg gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg
     7201 cgtattttga ttacactcct gttaatccat acagcaacag tactggggta acctggtact
     7261 gttgtccgtt ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt tcttgagcca
     7321 ggaatacact gaagacaaag aagtcacatt gacaaagtta agtagcggcc gccgccttct
     7381 ggaggcattg ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag
     7441 ctttaaccct tcggacccca gctggtcgca aacggcctgg catgaaccta tccataattt
     7501 aggtgggatg cccggtgcgt ggttggcaga tacgctgttc tttatttttg gcgtgatggc
     7561 ttacaccatt cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag
     7621 cgacgaatac attgattatt ttgccgtttc gctacgcatc attggcgttt tggcgctcat
     7681 ccttacctcc tgtggtctgg cggcaatcaa cgctgacgat atctggtatt ttgcctccgg
     7741 tggcgtcatt ggcagcttac taagcactac gctacaacca ctgctacaca gtagcggggg
     7801 aactattgcg ctgctctgcg tttgggcagc gggcctgacg ttgttcaccg gttggtcatg
     7861 ggtgaccatt gctgaaaaac tcggcggctg gattttaaac attctcacct tcgccagtaa
     7921 tcgtacccgt cgcgatgata cctgggtcga tgaagatgag tatgaagacg acgaagagta
     7981 tgaagatgaa aatcacggca aacagcatga atcacgccgt gcccgtattc ttcgcggcgc
     8041 gctagcgcgt cgtaaacggt tggcggaaaa attcattaat ccgatggggc ggcaaacaga
     8101 cgctgcgttg ttctccggta agcggatgga tgatgacgaa gagattacct acactgcacg
     8161 cggtgtggct gccgacccgg acgacgtcct attttcgggc aatcgtgcaa cgcagccaga
     8221 atatgacgaa tacgatccat tattaaacgg tgcgccaatt accgaacctg tcgctgtagc
     8281 agctgctgct accacggcga cacaaagctg ggctgcgccg gttgaacctg tgactcagac
     8341 gccgcctgtt gcctctgttg atgttccacc ttcgcaacct acagtagcct ggcagcctgt
     8401 accgggtcca caaacgggag agccggttat tgctcctgca ccggaaggtt acccacagca
     8461 gtcacaatat gcgcagcctg cagtgcaata taatgagccg ctgcaacaac cagtacagcc
     8521 gcagcagccg tattatgcac ctgcagctga acaacctgcg caacagccgt attatgcccc
     8581 tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt
     8641 tgctccacag tctacatacc agactgagca aacttatcag cagccagccg ctcaggagcc
     8701 gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg aacccgttgt
     8761 agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg aagagaagcg
     8821 agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga
     8881 accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac ctccagtaga
     8941 agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg
     9001 ggctgccgca accgttgccg cgccagtctt cagtctggca aatggcggtg gaccgcgtcc
     9061 gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac
     9121 tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagaaaa
     9181 agcccgtgaa gcccagcgca atcagtatga ttctggcgat cagtataacg atgatgaaat
     9241 cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc agcaacgcta
     9301 tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga
     9361 ggctgaactg gctcgtcagt ttgtgcaaac tcaacaacaa cgttattccg gcgaacaacc
     9421 ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct
     9481 ggatgatggt ccacacgaac cgctgtttac gccaattgtt gaacctgtac agcagccgca
     9541 acaaccggtt gcaccacagc agcagtatca gcagccacaa cagccggttg cgccacagcc
     9601 gcagtatcag cagccacaac agcaggttgc gccgcagcca caatatcagc agccgcaaca
     9661 accggttgcg ccacagcagc aatatcagca gccgcaacaa ccagttgcgc cgcagccgca
     9721 gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc cacaacagcc
     9781 agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta atggcgacag
     9841 ccgtccgttg cacaaaccga cgacgccgct gccttctctg gatttgctga caccgccgcc
     9901 gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggcgcgtc tggtggaagc
     9961 gcgtctggct gatttccgta ttaaagccga tgtcgttaat tactctccgg ggccagttat
    10021 cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc
    10081 acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg
    10141 caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt atctgcgcga
    10201 agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa
    10261 agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc
    10321 ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta
    10381 taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc
    10441 ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga aagatgccgc
    10501 caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga tgtctgcgct
    10561 gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg
    10621 tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc atccggtgct
    10681 gaaaaaagaa ccatacattg tcgtgttggt tgacgaattt gccgacctga tgatgacggt
    10741 aggtaaaaaa gtggaagagc tgatagctcg tctggcgcaa aaagcccgtg ccgcgggtat
    10801 ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc tgattaaagc
    10861 gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct
    10921 tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact ctgggccgaa
    10981 ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt
    11041 ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca cctccgacag
    11101 cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc cgttgttcga
    11161 tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg tacagcgtca
    11221 gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaggggat
    11281 tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta
    11341 attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa
    11401 ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag tagagggaac
    11461 tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgct atcacctgtg
    11521 cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa
    11581 gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg actgacggta
    11641 gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca aacttattca
    11701 actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa acactgtggt
    11761 tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc accggtaata
    11821 cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac aatatcaaac
    11881 agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg aagcagttca
    11941 ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag caggacgatc
    12001 agcgcagcag ttatcagctg aaatcccagc aaaatggggc tgtggatgca gcgaaattta
    12061 ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg cacctgagtg
    12121 agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc gcgtatgcgg
    12181 ccagaaaatt tagcacagta tatcggtcag caacatttgc tggctgcggg gaagccgttg
    12241 ccgcgcgcta tcgaagccgg gcatttgcat tctatgatcc tctgggggcc accgggtacc
    12301 ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt ggaacgtatt
    12361 tctgccgtca cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc ccggcaaaac
    12421 cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt caacaaaagc
    12481 cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat tggcgcaacc
    12541 actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg tgtttatctg
    12601 ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat ggaagacaaa
    12661 acccgtggct atggtggtca ggatattgtt ctgccagatg atacacgacg tgccattgca
    12721 gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg
    12781 gccgaagtct atgatagcgg taagcgggtc ctgaagcctg aattactgat cgaaatcgcc
    12841 ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct aatttccgca
    12901 ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta tgcgcgaatt
    12961 attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat tgcgtctgaa
    13021 gacgtcggta atgccgatcc acgggcgatg caggtggcaa ttgcggcttg ggattgcttt
    13081 actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt ttacctggcc
    13141 tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc cgatgctcgc
    13201 gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa attaatgaag
    13261 gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc ttatgctgcc
    13321 ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc gacaaacagg
    13381 ggccttgaag gcaagattgg tgaaaagctc gcctggctgg ctgaacagga tcaaaatagc
    13441 cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt atccctgtgg
    13501 tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag catgctcgat
    13561 cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg ccggggcttt
    13621 aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa
    13681 acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg
    13741 cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga agagctggat
    13801 gcagcaaaag ccgagctgga tgctttacag gctgaaattc gcgatatcgc gctgaccatc
    13861 cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa cgttgaagtc
    13921 agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt gacgctgggt
    13981 gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc ccgctttgtg
    14041 gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat gctggatctg
    14101 cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa ccaggacacg
    14161 ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac tcgtccgctg
    14221 gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt tccgctgact
    14281 aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat gatcgcccac
    14341 accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg tctgatccgt
    14401 atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccagaaga ctcaatggcg
    14461 gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg cctgccgtac
    14521 cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac ttacgacctg
    14581 gaagtatgga tcccggcaca gaacacctac cgtgagatct cttcctgctc caacgtttgg
    14641 gatttccagg cacgtcgtat gcaggcacgt tgccgcagca agtcggacaa gaaaacccgt
    14701 ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt tgcagtaatg
    14761 gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg tccgtatatg
    14821 aacggactgg aatatattgg ctaataccca atttttctga atctaaaaag cgcctttagg
    14881 gcgctttttt tgtctccctt tgataccgaa caataattac tcctcactta cacgtaatac
    14941 tactttcgag tgaaaatcta ccgatctctt tgattttcaa attattctat gtatacaagc
    15001 ctatatagcg agctgctata gaaataatta cacaatacgg tttgttactg gaatcaatcg
    15061 tgatcaagct tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta ttggctgctg
    15121 aggagagtcg ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca atggccagca
    15181 gcgcattaac attacctttt agtcggattg cgcacgctgt cgatagcgcc attccaacaa
    15241 aatcagacga aaaggttatc tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc
    15301 tacgtatgca cgtcgtggac ggtgaaatca aatatgtcga aacggacaat accggcgatg
    15361 acaattacga cggcctacac taggttcgcg cctgcctgcg tgggcgttcc atgcgtcacc
    15421 gtgtctacaa tccggaccgc ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag
    15481 gcaaattcga gcgcattagc tgggaagaag cctacgacat catcgcgacc aatatgcagc
    15541 gcctgatcaa agagtacggc aacgagtcta tctatctgaa ctatggcacc ggtacgctgg
    15601 gcggcaccat gacccgctcc tggccgccgg gaaataccct ggtcgcgcgc ctgatgaact
    15661 gctgcggcgg ttatcttaac cattacggtg actactcttc cgcgcaaatt gcggaaggtt
    15721 tgaaccatac ctacggcggc tgggcagatg gtaacagccc gtcggatatc gaaaacagta
    15781 aattggtagt gctgtttggt aataacccag gcgaaacgcg aatgagtggc ggtggggtga
    15841 cttactatct tgaacaggca cgccagaaat ctaatgctcg catgatcatc atcgatccgc
    15901 gctataccga caccggtgcc gggcgcgaag atgagtggat ccctattcgt ccaggaacag
    15961 atgccgcact ggttaacggt ctggcgtacg tcatgatcac taaaaacctg gtggatcagg
    16021 cattcctcga taaatattgc gttggctatg acgagaaaac cctgcccgcc agtgcgccga
    16081 aaaatggtca ttataaagct tatattctgg gtgaagggcc agatggcgtg gctaaaacac
    16141 cggaatgggc ctcgcaaatc accggtgttc cggcagacaa aatcatcaaa ctggctcgtg
    16201 aaatcggcag taccaaaccg gcgtttatca gccagggatg gggcccgcag cgtcacgcta
    16261 acggtgaaat cgcaacccgt gctatctcga tgctggcgat tctgaccggt aacgttggta
    16321 ttaatggagg gaacagcggc gcgcgtgaag gttcatacag cttaccgttt gtccgtatgc
    16381 cgaccttgga aaacccgatc cagaccagca tttcgatgtt tatgtggacc gatgccattg
    16441 aacgtggccc ggaaatgacg gcgctgcgtg atggtgtgcg cgggaaagat aagctggatg
    16501 tgccgatcaa aatgatctgg aactatgccg gtaactgcct gattaaccag cattctgaaa
    16561 tcaaccgtac ccatgaaatc ctccaggatg ataagaagtg cgagctgatt gtggttatcg
    16621 actgccacat gacctcatcg gcgaaatatg ctgacatcct gctgcctgac tgcaccgctt
    16681 ccgaacagat ggactttgca ctggatgcat cctgcgggaa tatgtcttac gtgattttca
    16741 acgatcaggt gattaaaccg cgctttgaat gtaagaccat ctatgaaatg accagcgaac
    16801 tggcaaaacg tcttggcgtt gagcaacagt ttactgaagg ccgtacctag gaagagtgga
    16861 tgcggcatct gtatgcccag tcgcgggaag cgattcctga actgccaacg tttgaagagt
    16921 tccgcaagca ggggatcttt aaaaagcgcg acccacaagg gcatcacgtt gcttataaag
    16981 ccttccgtga agatccgcag gcaaacccac tgactacgcc atcgggcaaa attgagattt
    17041 attcgcaggc gctggctgac attgccgcta cctgggaatt gccagaaggc gatgtgatcg
    17101 atccactgcc gatctacacg ccgggctttg aaagttatca ggatccgctg aacaaacagt
    17161 atccgctgca gcttacaggt ttccactata aatctcgcgt tcactcaact tacagcaacg
    17221 ttgatgtgct gaaagcggct tgccgtcagg aaatgtggat caacccgctt gatgcccaaa
    17281 aacgcggtat ccacaacggc gataaagtca ggatctttaa cgatcgtggt gaggttcata
    17341 ttgaggcgaa agtgacgcca cgaatgatgc cgggcgtggt cgcactgggt gaaggcgcct
    17401 ggtatgaccc ggatgcaaaa cgtgtcgata agggcggttg tatcaacgta ctgaccactc
    17461 aacgtccgtc tcctctcgct aaggggaatc cgtcacatac aaaccttgtt caggttgaaa
    17521 aggtgtaagg agtaaccgat gacaacccag tatggatttt ttattgattc cagccgttgc
    17581 accggttgca aaacctgcga gctggcttgt aaagactaca aagatttgac gccagaagtc
    17641 agtttccgcc gcatttatga atatgctggc ggcgactggc aggaagataa cggtgtctgg
    17701 caccagaacg tgtttgccta ctatctgtcg atttcgtgca accactgcga agatccggct
    17761 tgtactaaag tctgcccgag cggtgcgatg cataaacgcg aagatggttt tgttgtggtc
    17821 gatgaagatg tatgcattgg ctgccgttac tgtcatatgg cttgcccgta tggcgcaccg
    17881 caatataacg aaacgaaagg ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg
    17941 gagggtaaaa agccgatctg tgttgaatct tgcccgctgc gggcgctgga tttcggccct
    18001 atcgacgagc tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt gccgcgagct
    18061 cactttacca aaccgaatat tgtgatcaaa cccaatgcca atagccgccc gaccggggat
    18121 accactggct atctggcaaa cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg
    18181 gccgctgatg atcttcacgg tcttcgggca atgtgtggcg ggtggtttta tcgttctggc
    18241 tttggcgctg ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta tcgcctgcat
    18301 gtttggctta tgggtactga tgggcattgg ctttatcgcc tctatgctcc atcttggttc
    18361 accgatgcgc gcctttaact cgctcaaccg ggtaggggct tcagcactca gtaacgaaat
    18421 cgccagcggt tcgatctttt ttgctgtagg cggcatcggc tggttgctgg caatgctgaa
    18481 aaagctgtca ccggcattgc gtacgctatg gctggtggtg acgatggttc ttggcgtcgt
    18541 ctttgtctgg atgatggtgc gtgtgtataa cagcattgat accgttccga cctggtacag
    18601 catctggacg ccgatgggct gggcgatgcg tctgctgcca gccatttctg tactggcact
    18661 ggtggtgagt ggcgtgatgt cagtgatgca gggcacagag ctggcaacta ttcatagttc
    18721 tgtgcagcag gccgcagcgc tggtgccgga ctatggtgcg ctgatgtcct ggcggatcgt
    18781 gcttttggcc gttgccctgt gcttgtggat tgcaccacag ctaaaaggtt atcagcctgc
    18841 ggttccgcta ctatccgtct cattcattct gcttctggca ggggaattaa tcggtcgcgg
    18901 cgtattctat ggtttgcata tgaccgtggg gatggccgtc gcaagctaat cagtaacaac
    18961 cggggtttcg gccccggttc tcttttattt ctgcttcgtt aacgtgtcat aactggtcat
    19021 caaattacga taatcaggaa tatggttaga gaacaacgtc gccagtcctt caatatcatt
    19081 acgccagtca cggtgcagct cgcaagccac gccgaaccat gtcatcaatt gcgcaccagc
    19141 ttgcgacatg cgatcccacg ccgaatgccg ggtaatttca ttaaaggtcc ctgatgcatc
    19201 agtcacgaca aagacatcaa acccttcttc aatggctgaa agcgccggga atgccacgca
    19261 aacttcggtt accacaccgg caataattaa ctgtttttta cctgtcgctt tgactgcttt
    19321 tacaaaatct tcgttatccc aggcgttaat attgcccggg cgagcaatgt aaggtgcatc
    19381 ggggaattgt gctttcagtt ctagaactaa tgggccgtta ggaccggttt caaaactggt
    19441 ggtaagaata gttggtaagt tgaaatactt agccaggtca cccagcgcca gcacgttatt
    19501 tttaaactta tcgggttcga tatcccgtac aagggaaagt aaaccagcct ggtgatcaac
    19561 aagcaaaacg gcagcatcat ttttatcaag acgaacatac ggtttggtca tcctcttctc
    19621 ctttcgagag tcagtggtac gtctgaaatc ggtcagacgc gatgcattgc tctgaaagca
    19681 tagacgggaa atatgagttt gctgtgacca tgaaattttt cgactgaacg aagcgtatga
    19741 aatttgtgtt agatcaataa aaacaatcag atgagattaa tcatctcttc agcattagcg
    19801 ttatgaatga gtaatataaa tcgataatac cgccgctgcg atatgcgcgt attttcaccc
    19861 attgacaatg tttttggcgg tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt
    19921 aattcatgtc cacgtatacc cggcctgtca tgcttttgct gtctggcctg cttttgttga
    19981 ctctggcgat cgcggtgtta aatacactcg tgccgctttg gctcgcccag gaacacatgt
    20041 ccacatggca ggtaggcgtt gtcagctcat cctattttac cggcaacctt gtcggtacat
    20101 tgctgacagg gtatgtcatt aaacgcattg gctttaaccg cagctattat ctgtcctcct
    20161 tcatttttgc tgctggctgt gccggccttg gcctgatgat tggtttctgg agctggttgg
    20221 cgtggcgttt tgtcgcgggc gtcggctgtg ccatgatttg ggtggttgtt gagagcgcgc
    20281 tgatgtgcag tgggacgtca cgtaaccgtg ggcgtttgct tgctgcgtat atgatggttt
    20341 attacgtggg aacgttttta ggccagttac tggtcagcaa agtctcaacc gagctgatga
    20401 gcgtattgcc gtgggttaca ggtttgacat tggcagggat cttaccgctg ttgtttacgc
    20461 gtgtcctgaa tcagcaggct gaaaaccatg attcgacgtc aattacggca atgctaaaac
    20521 tccgtcaggc gcggcttggc gtgaatggct gcattatctc aggaatcgtt ctgggatctc
    20581 tatatggcct gatgcctctg tacctcaatc acaaaggggt gagcaatgcc agtattggtt
    20641 tctggatggc ggtactggtc agtgcgggta tcctcggaca atggccgatt ggacgtctgg
    20701 cggataagtt tggtcgactg ctggttttgc gtgttcaggt ctttgtcgtc attctcggca
    20761 gtattgcgat gcttagccag gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg
    20821 gctttacgct atatccggtg gcgatggcct gggcttgtga gaaagttgaa catcatcaac
    20881 tggtggcgat gaaccaagcc ttactgttga gctataccgt gggaagtctg cttggcccgt
    20941 catttagcgc tatgctaatg cagaatttct ccgataattt attgtttatc atgatcgcca
    21001 gcgtatcgtt tatctatttg ctgatgctgc ttcgcaacgc cggtcatacg ccgaaacccg
    21061 ttgctcacgt gtaaatgaat tcaagcaaag tgtgaactta ctgtttcaca ctctgctttt
    21121 ttgtttcttc tatctgactt gcttttttcc aaattttatt cgtttaaaaa taaaatgtgc
    21181 agcaggttat aattttgcat ttcgctattt ccgcacttct tatttgccgc gcataatccc
    21241 tcgttttaca gatgcccctt taattttggc gaaggatttg tctatggctg ggaatgttca
    21301 g
//
LOCUS       DBKELA010000067        21275 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.067, whole genome shotgun sequence.
ACCESSION   DBKELA010000067 DBKELA010000000
VERSION     DBKELA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.067"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..445)
                     /locus_tag="ACUQR0_002534"
     CDS             complement(<1..445)
                     /locus_tag="ACUQR0_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HHV9109522.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(500..1351)
                     /gene="manZ"
                     /locus_tag="ACUQR0_002535"
     CDS             complement(500..1351)
                     /gene="manZ"
                     /locus_tag="ACUQR0_002535"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HHV9109523.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1364..2164)
                     /gene="manY"
                     /locus_tag="ACUQR0_002536"
     CDS             complement(1364..2164)
                     /gene="manY"
                     /locus_tag="ACUQR0_002536"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HHV9109524.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2227..3198)
                     /gene="manX"
                     /locus_tag="ACUQR0_002537"
     CDS             complement(2227..3198)
                     /gene="manX"
                     /locus_tag="ACUQR0_002537"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HHV9109525.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3515..3673
                     /locus_tag="ACUQR0_002538"
     CDS             3515..3673
                     /locus_tag="ACUQR0_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HHV9109526.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3661..5211
                     /gene="yoaE"
                     /locus_tag="ACUQR0_002539"
     CDS             3661..5211
                     /gene="yoaE"
                     /locus_tag="ACUQR0_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HHV9109527.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5215..6813)
                     /locus_tag="ACUQR0_002540"
     CDS             complement(5215..6813)
                     /locus_tag="ACUQR0_002540"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HHV9109528.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6944..8308)
                     /gene="sdaA"
                     /locus_tag="ACUQR0_002541"
     CDS             complement(6944..8308)
                     /gene="sdaA"
                     /locus_tag="ACUQR0_002541"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HHV9109529.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8492..9070)
                     /locus_tag="ACUQR0_002542"
     CDS             complement(8492..9070)
                     /locus_tag="ACUQR0_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HHV9109530.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9074..10435)
                     /gene="pabB"
                     /locus_tag="ACUQR0_002543"
     CDS             complement(9074..10435)
                     /gene="pabB"
                     /locus_tag="ACUQR0_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HHV9109531.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10509..10688
                     /locus_tag="ACUQR0_002544"
     CDS             10509..10688
                     /locus_tag="ACUQR0_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HHV9109532.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10808..11107)
                     /locus_tag="ACUQR0_002545"
     CDS             complement(10808..11107)
                     /locus_tag="ACUQR0_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HHV9109533.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11529..11873)
                     /locus_tag="ACUQR0_002546"
     CDS             complement(11529..11873)
                     /locus_tag="ACUQR0_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HHV9109534.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12005..13915
                     /locus_tag="ACUQR0_002547"
     CDS             12005..13915
                     /locus_tag="ACUQR0_002547"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HHV9109535.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13973..14668
                     /gene="tsaB"
                     /locus_tag="ACUQR0_002548"
     CDS             13973..14668
                     /gene="tsaB"
                     /locus_tag="ACUQR0_002548"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HHV9109536.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14708..15289
                     /gene="yeaY"
                     /locus_tag="ACUQR0_002549"
     CDS             14708..15289
                     /gene="yeaY"
                     /locus_tag="ACUQR0_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HHV9109537.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="ACUQR0_002550"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="ACUQR0_002550"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HHV9109538.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="ACUQR0_002551"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="ACUQR0_002551"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HHV9109539.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACUQR0_002552"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACUQR0_002552"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HHV9109540.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20080)
                     /gene="yeaW"
                     /locus_tag="ACUQR0_002553"
                     /pseudo
     CDS             complement(19451..20080)
                     /gene="yeaW"
                     /locus_tag="ACUQR0_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(20410..21018)
                     /locus_tag="ACUQR0_002554"
     CDS             complement(20410..21018)
                     /locus_tag="ACUQR0_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9109541.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="ACUQR0_002555"
     CDS             21188..>21275
                     /locus_tag="ACUQR0_002555"
                     /inference="COORDINATES: protein motif:HMM:NF025073.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9109542.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5267 a         5273 c         5466 g         5269 t
ORIGIN      
        1 cgagaagctt ataaattttt tccagatcgt cgatatttcg aacgcggatt aacagccgac
       61 gctgttctaa ttgcatcacc aacacgccat cttccgacaa gttcatcgct ttgattcggc
      121 tatattcaat ccagacattg gcgaagaaaa aacctttttg tttaaagatg atcttcggaa
      181 tgcggatcca gaatatataa aaacccatca gagccagtgc acttaataac catgttgtta
      241 ttagcgcgcc atgattcgtg acgttgttat agataagaat gacaatcaat ccgacgaaga
      301 taacgctatc gatgcgacca cgccggagca aaggaattgc cagcagggtg gggccgttac
      361 ggcggggcat gatgaactga tcgtagatcg cgaaggccag gagtgcggcg atgaaaagaa
      421 tcagtaccag gtccgtgatt gtcattaatc ctccagataa aaaaacgggg ccaaaaggcc
      481 ccggtagtgt acaacagtct tacagtccca gcaggccgca agcgtaacca gcgataccga
      541 tgacgaagaa gccaacgatg atccacagcg ggttaacttt tttgcgcagc agccacatac
      601 aagcaaatgt cagcagcagt ggtacaaggc ctggcattaa ctggtcaaga atagtttgca
      661 cggtggtgac acgggtctgt ccatcctgcc cggtgatggt cgaaaccacc agcgggatgt
      721 tgacatgtgt ccacttgtta accaatgccc ccatgacaaa caggccgagg atagacgccc
      781 cttccgtcag tttttgcagg aagccaccac ccatatcttt aacgatatcg atacctttgg
      841 agtaaccata cgctacgccg tagtaacggg ttgccagacg caccaggtta aacaggatga
      901 agaacagcag cggacctaac aggctgccgc tcatcgcgat accggcaccc agtgctgcaa
      961 ataccggacg tacggttccc cagaagatcg ggtcgcctac accagccagc ggccccatca
     1021 aaccgacttt gataccgttg atagcaccgt catcgatctc tgcgccatta gcacgctgtt
     1081 cttccagcgc cagggttacg ccgagaatcg gtgcagccac gaacggctgg gtgttaaaga
     1141 actccaggtg acggcgaata gcttgtttac gagcttcgtt gttctcaggg tagaggcgac
     1201 gaattgccgg taccatagag aagcagaaac ccagtgcctg catacgttcg aagttccatg
     1261 aaccctggaa gaggttagaa cgcaggaata cgccacgaat atcactttga gtgagttttt
     1321 tctcggtggt agtttgagtt gtatcaacca tttcgctcac ctgttagtcc agttcgttat
     1381 cgagatcgtt gttaccagct gcctgagcag gcgcaccggc tacgcggttg tatttcgggc
     1441 taagttggat gtagagcact gccataacag taccaatcac acccagagca accaggttaa
     1501 agttggtgaa tgctgcggtt acgaagccga ggtagaagaa cggcatcagg tagccagcac
     1561 gcatcatgtt gataaccatc gcataaccaa ccaccacgat catgcccccg gcgatattca
     1621 gaccattggt caccacttcc ggaatcgcat tcagcatgtt ctgtacttcg ctggtaccaa
     1681 cagacagcgc aacgatgacg gccggaatag ccacacgcat tgcttgcagg aacagagaag
     1741 aaacgtggat ccaggaaatc gctgtcaggt tgccgttatc agcagcctta tccgcagcgt
     1801 gctggaaagc aacggtaata gtacgaacga tgatggtcag tacctggccc gcagcggcca
     1861 gagggattgc cagtgcgata cctgcaccaa tgctctgatg acctgcgata accagaatgg
     1921 tagaaatgat agaagccaga gcggcgtcag gcgcaactgc agcaccgatg ttcatccagc
     1981 ccagcgcgat catttccagc gtaccaccga taataatacc ggttttcata tccccaagaa
     2041 cgatacccac cagggtacac gcgattagcg gacggtgaaa ctgaaattca tcgaggattg
     2101 atcccatacc tgcgatacag gctacgataa ataccagcac aatttgaaga gtggtaatct
     2161 ccattgtact tctcctgttt acgtaagact taagtgtgaa aactgaatga taatcaacac
     2221 aatacgttac ttatcgattt tgctgatcag atccatcatt ttcagtttcg gatcggtgga
     2281 aaccttacgg acttccagct caataccgcg tgcattcagt tttttgaacg cctcgatatc
     2341 tttttcatca accgaaaccg cgttattcac ctgggtttta ccctgacgga atgccatacc
     2401 accgacgtta acagaggtga ttttcacgcc gccttcaacg agacgctcta catctgttgg
     2461 gttggtaaat aacagcatta cgcgttcgcc agcatatttc gggttgttgt agacgcgaat
     2521 cattttggca acatcaacta cgtgtgctgt tacgcccgga ggtgcaacct gggtgagcag
     2581 tgtcttacga acggtatccg cagccacttc atcactaaca acaataatac gggagacatt
     2641 ggtttctttg gtccagcggg tggcgacctg accgtgaatc agacggtcgt cgatacgcgc
     2701 aaggccaata accatgtagt cgtttggcac cattggtttt gccggagttg gagccgcttt
     2761 tggtgctgcg gcagcgggtg ctggcgcggc tttttcaacc ggtttggctt tcagtgcttt
     2821 cacgccttca cggcctgttt ctactgccag cgcaaccagt tcatcaaagc tcgggtcatc
     2881 atcacgggcc attaacgttt ccacgagcat tggaatgtta acgcctgcaa tgacttcata
     2941 atgctctttg tcgacgacaa tgcggctggc agcattgaac gggctgcctc cccatgtatc
     3001 aacgagaaac agcacgcctt tagtggtgtc gagttttgcc aactgagcgt tgtatttttc
     3061 aatcagcgtt tcggcatttt cacctggaac gaaatcgatc cagccgacgt tttcctgctc
     3121 gcctaacagc atttctgccg ttttaagcaa ctgctctgca gcccaaccat gtgtgcctat
     3181 aacaatagca atggtcactt gctacctcct ttattatcgt taacacctca acgtgccaga
     3241 tgtatttttg aatcgtcgtc cacaatcgaa tcgattcaga taagggctac aaccaaaaac
     3301 ccctgcgttt cgcgatttat tttagatatc gaaaaaatta ttttatgtga tgaagatccg
     3361 taatttaact ttcgattatc agaaatttcg aaaggtaaaa tatccttgtc acattcgttt
     3421 gcaaaggaag gtaaatcttt gccaaatcag aggcgtctct gatatgttta actcccgttt
     3481 aacaaccatg gagtataggg cagtagcccg cagtatggat cgtcaccgac gtcatttcag
     3541 catcaggcct tttaacgcct gcctttctgg cactctatgc cgcacctttc gtttgcattt
     3601 tgtcgttacg cctgcattat ttctggcgtc gaatagctat tccttaagca ggagcttgtc
     3661 atggaattct taatggaccc ctcaatttgg gcggggctac tcacgcttgt tgttctcgaa
     3721 attgtgctgg gtatcgataa cctggtcttc atcgccattc ttgctgacaa actgccgcca
     3781 aaacaacgcg ataaagcgcg tttgctgggg ttatcactgg cgctgattat gcgtctgggg
     3841 ctgctgtcgc tgatttcatg gatggtcacg ctgaccaaac cgctatttac cgtcatggat
     3901 ttctccttct ccggacgcga tctgattatg ttgttcgggg ggatattctt gctgttcaaa
     3961 gcaacaaccg aactgcatga acggctggaa aaccgcgatc atgattccgg ccacggtaaa
     4021 ggctacgcca gtttctgggt ggtcgtcaca cagattgtca tccttgacgc cgtcttctcg
     4081 ttggatgcgg taattactgc agtagggatg gttaaccatc tgccggtgat gatggcggcg
     4141 gtagtgattg cgatggcggt tatgttgctg gcatcgaaac cgctgacgcg attcgttaac
     4201 cagcacccca cggtggtggt gctctgtctg agcttcctgt taatgattgg tctgagtctg
     4261 gtggcagaag gtttcggttt ccacattccg aaaggttacc tgtatgccgc gattggcttc
     4321 tcgatcatta ttgaagtgtt taaccagatt gcgcgtcgca actttattcg tcaccagtcg
     4381 actttgccgc tgcgagcgcg tactgccgat gccatcctgc gtttgatggg cgggaaacgt
     4441 caggccaatg ttcagcacga tgccgataac ccgatgccga tcccggaagg tgcatttgcc
     4501 gaagaagaac ggtacatgat taacggcgta ctgacgctgg cgtcgcgttc tctgcgcggg
     4561 atcatgacgc cgcgcggtga aataagctgg gttgacgcta atctcggggt cgatgaaatc
     4621 cgcgagcaac tgctctcttc accgcacagt ctgttcccgg tatgtcgcgg tgaactggat
     4681 gaaatcatcg gtatcgtacg tgctaaagaa ctgctggtgg cgctggaaga gggcgttgat
     4741 gtggcggcga ttgcttcggc gtctccggcg attatcgtcc cggaaaccct cgatccgatc
     4801 aacctgctgg gcgtgctgcg tcgcgctcgc gggagctttg ttatcgtgac caacgagttt
     4861 ggtgtggtac aaggcttggt cacgccgctg gatgtgctgg aagccattgc gggtgaattc
     4921 ccggacgctg acgaaacgcc ggaaatcatt accgatggta acggctggct ggtaaaaggc
     4981 ggtacagatt tgcatgcctt gcagcaggcg cttgatgttg agcaccttgc cgatgacgat
     5041 gatatcgcga cggtcgcggg cctcgtgatc tcggcaaatg gtcacattcc ccgtgtgggc
     5101 gatgtgattg atgtagggcc actgcatatc accatccttg aagccaatga ttatcgtgtt
     5161 gatctggttc gcattgttaa agagcaaccg gcgcacgatg aagatgagta agcgttaacg
     5221 taacgacata atgggcgtga tatgtccatt atgccgggcg ggcggcggtt ggctgcccgc
     5281 cagccatttg ggaaaatcac gtagcggcat cggctgtgcg tacaaatacc cttgcaaaat
     5341 atgcacgcca tgacggcgca aatacttcgc ctgttcttgc gtttccacgc cctccgccac
     5401 cagttcaata ttcagtcttt gccccagcgc gatgatgata tcggtcaccg tcgagttaac
     5461 cgcgtcagaa cctatagctg cggtaaatga cttatcaatt ttcagcacgt caggacgtaa
     5521 tgtttcaagc caggaaaacg agctgttgcc ggtgccgaag tcatcaatcg ccagtttgac
     5581 gtttttacga tgcagctcgc gggcaatccg ataatcaaca tccagtaagg catcgcgttc
     5641 ggtgatttcg aggatcagtt gctgaatcgg gtgagcgcta aaccagtact gattgagatc
     5701 ttttatcagc acaccacggc gaaaatggct gggtgcgacg ttaatgccaa catgaaactg
     5761 actactcatc gggaaaacat ggtgctgacg aatggtttct gccatcacat agtgggtcag
     5821 tggcacaatt aaatgatgtt cttccgcgat aggaataaac acatccggtg aaatccagcc
     5881 ctgacgcgga ttattccagc gcagcaaaat ctctacacca atacattgct ggctgcgcgc
     5941 attaagcaaa ggctgacaga acaattcgaa ctcatgttgc gccaggccca gattaatttc
     6001 gcgggaaaag ctcatccggt aagcggtcgc cagccaggcg atatagccca ccagtaaact
     6061 tagcagcacc gccagcggta attgtgttgg aagatagtgc catgccagcg ccgtagcccc
     6121 cggaccattt acgttaatgg taaaaggaaa gctttgcgaa gaaacctgat agttttcatt
     6181 gtcttcaggt tgcggaaggg aatctaccag cccattacca tagagcaaat gccgtttgtc
     6241 caccgtcagg ctggcgctac tgatttgcgg cagttgtggc tcaaggagca ttgccgtcag
     6301 taagtcgatg ttaatcatct ctatgacccc agcattgcta ctgcccgctg caggcgtcca
     6361 ttgaatcaaa accggactgc ctttaatcag ggcacggtcg atcgtcaggc gtaaaagtgg
     6421 ttgcggtgca ggaagttcag ccagaatgtc cacgacgggc acattgcgat aaccaaaaat
     6481 gctggagcaa taaagtgtgc cgtcttgcac caggccaatg gatcgcacag tttggagttt
     6541 tgccgcctgt ttacgtaatg gcaaatgggc gacagagcag ggcagaccaa tcagcggaag
     6601 caagactttg ctacctgcct gtaggggaag cagtacttta tctaattcct ctacagcgtg
     6661 attggcgaat tgtactaccc gttgttgatt taagttacgc tgtgaaataa atcgcacgct
     6721 cagggtcgaa gcgagcgtaa cgagtgcgca aatcgtacaa acaatcattc gattacggcg
     6781 ataggtttta atgatccgtt gtgctttttg catgcgggcc accctgtaag cgtatggcga
     6841 caaacgcctg acgcaacagt ggaagtgtag tggggaatat tacagcagac gagaaagcgg
     6901 gtataaattc gcccatccgt tgcagatggg cgagtaagaa gtattagtca cactggactt
     6961 tgattgccag accaccgcgt gaggtttcgc ggtatttggc gttcatgtcc ttaccggttt
     7021 cgtacatcgt ttcgatgacc ttatccagcg agacgcgcgg tgcactggtg cggcgcagag
     7081 ccatccgtgc ggcgttaatc gccttcacag aggcaatggc attacgctca atgcacggca
     7141 cctgaacctg ccctgcaacc gggtcgcagg ttaaaccaag gttgtgttcc atgccaattt
     7201 ccgccgccac gcaaacctgt tccgggctac cgcccagcag ttctgcaaga cccgcagcag
     7261 ccattgaaca ggcaacaccc acttcgccct ggcaaccaac ttccgcaccg gaaatagagg
     7321 cgttcatttt atacaatgca ccaatcgcgc ccgctgccat aaagtaacgg gtatagatgt
     7381 ctgggctgac cgattcaata aagtggtcat agtaagccag cactgccgga acgataccgc
     7441 aggcaccgtt ggttggcgca gttaccacac gaccaccggc ggcgttttct tcgttaactg
     7501 ccagcgcaaa catgtttacc cagtcaatga cattcatcgg atcgttagac agtttatcgc
     7561 tggaaactaa catccggcgc agggcagacg cacgacgtgg cacgcgcagc gggcctggca
     7621 gtacaccttc ggtgttcatc ccgcgatcga tacatgcctg catggtttgc cagacatgcg
     7681 cgaaatactc gtcgatctct ttcttgctgt gcagcgccag ttcgttctgc atagcgagac
     7741 cagacagcga atagccggtt tcattacagt acgcgagcag ttcggtggca gatttgaacg
     7801 gatacggcac gctcacttcg ttggcagcat cctgaccaaa gtgttcttca tcgacgataa
     7861 aaccgccgcc gatggaataa taagttttgc tgtagacgac ttcatcgccg ttataggcgt
     7921 ggatttgcat accgttttca tgcagcggca ggttgccgtt atgaaaacgc atcccgttgt
     7981 cgcgcgggaa atccacttca tgccgtccct gtgccagcag cagacgttcg cgctctttta
     8041 cgtcgcgaat aaaaccggga atactgtcga tatccacggt ggcaggttcg ttacctgcaa
     8101 gacccataat aatggcgata tcggtgtggt ggcctttacc cgtcagcgac agtgaaccat
     8161 aaacgtccac ggcaacgcga gtaacgctat ccagtaagcc tttttcgacc agatcatcga
     8221 cgaactgttt acccgccttc ataggcccta cggtatggga agatgaggga ccaatcccca
     8281 ccttaaacat gtcgaataga ctaatcacga taatactcct gacaaggtga ctggacttcc
     8341 agtaacgaac taataacagc gcatagtgta agagggaacg cggcgactgg cttaactatt
     8401 cacatgaatt aaactaatca aaaaccgcga tttaactaat attgcgatga ttgtctcaag
     8461 tagtgttttg tagatgtaaa gataagtata gtcaggggtt cacaccaatt tgcagcgcca
     8521 gctcacgaat tatgcctgcg gtcattcccc atacaaaata ctgttcgtac caggacagcc
     8581 atacccgatg tgaatcgcca cggcggtaga tatctaaagg gtgataacga cccagatgta
     8641 atgcctgggc gagcggcatt tcaaacaccg ccgagacttc atcttcactg gcgcgatacg
     8701 gcagatcggg cgggataatg ccgaccactg gggttacttg atagccagtt acgctatcga
     8761 cgggcggcag cacgccgata acttcaacgg cggaaggcgg tatagcgacc tcttcttcag
     8821 cttcgcgcag cgcggcggcg ataactgatg cgtccgtgtc atcgactgca cctccaggga
     8881 atgccacttg tccagcgtgt ttacgcagat gaattgaacg ctgagtcagc aacaaccccg
     8941 gttgcggtcg acggacgatg gggattaaca cggcagcctg acgatgattt aggggttccc
     9001 ggttaatttg cgggcgcaaa agttgaaagc gagataaaaa atcatcaagc gtcaggctac
     9061 ggtattccac gtcttacttc tccagttggc gtaatatctt attaacttta tcaaaagttt
     9121 cctgatattc cgcttcttcc tggctatcgg cgacaattcc accgcccgca gagcagtata
     9181 tttgtccgtt aatggcagtc agcgtgcgga tagtaatgct ggtatccatg ttgccgcaaa
     9241 agctcaaata gccaatgctg ccgcaccagg cattacgtcg ctgcggttcc agttcgtcga
     9301 taatttccat agcccgtact ttcggagccc cggttattga gccaccagga aaagctgcgc
     9361 gcagcagatc gctggcgtgt aactgttctg gtagtcgcgc cgttatagtg ctgaccagat
     9421 gatgcacggc agggaagggt tccaccacga agagctctgg tacttttacc gaaccggcta
     9481 cggcaacacg accgatatca ttacgcatta aatcgacaat catcagattt tcggcacgat
     9541 ctttcgctga gttcgccagt ttttctgctt gtttgctatc ttcctgagga tcgggcaggc
     9601 gtggtagcgt gcctttaatc gggcgggtct ggatttcact attatcacaa agaataaacc
     9661 gctctggcga aaggcttaaa attgcaccct gttcaagacg taaaaaagcg ctaaatggcg
     9721 cgcggttggc ctgattaagc tgaaggaatg cctgccattc atcgccagaa taggtcgcat
     9781 gaaaacgctg ggcgagattc acctgatagc aatcaccgct gtgcagatat tcctgtacct
     9841 ggcgaaattt ttcgccgtac tgctcgtggg tcatattgga ttgccagtcg gaagtgagcg
     9901 tgaaatcttc ctgcggcgag aattgctggc tttccagcca ggcccgacgg gcattgacat
     9961 cattatgact cagcaaagaa actgtatgac gctggtggtc gacaatgagc gcccaatcgt
    10021 agatacccac tgccatatcc ggcagaacga tatcttgttc cgcaatttct ggcagtgact
    10081 caaaacggcg gcccagatcg tagccaaaca accccagtgc gccgccctga aatggcaaat
    10141 cttcgttatg cgttgggcga atgtctgcgc gatccagcac ctgctggagc acctgtagcg
    10201 ggtcatcagt ggtcgttgtg cgtttttcgc tttcactaac aacggtttct ttacctaaag
    10261 tggttaaagt gcaaatcgga tcggcgacca caatatcaaa gcggctatac ggatgatcgg
    10321 catagccgga gtgtaaaagc atcgcccacg gcaggtggct taagcgggag aaataaaatt
    10381 cagcggcgtc ctgacgccag ggtaaagtaa tcacagtggg agataacgtc ttcatcagtc
    10441 ctgactctac tggctatgtg ccgggtagca tgaaataatt agcgccaaca atttagcagg
    10501 agttaacaat gtttgcaggt ttaccttcac tcatccatga acagcagcaa aaagctgtcg
    10561 agcggatcca ggaactgatg gcacaaggga tgagtagcgg ccaggcaatc gcgctggtgg
    10621 cagaagagct gcgtgccaac cactccggcg agctaatcgt ggcgcgtttt gaggatgaag
    10681 acgagtaaca cactaatccg tatcgatttc gttatagaag ccgatgatca tttgattgtg
    10741 agaaaaagga cgctttttat atgtgattaa aagcgtcctg acccatttat gctgatataa
    10801 atttaactca gaccagtgct tttagttctt cctgcatata tgttgaaaag tattcaggat
    10861 ttttaatcac agagcgttca cctgtctcca ttttttttgc ccatccaacc attttttccg
    10921 tgaacccagg gttccagcct tcctggtcag ctcgcgccgt aatatcagcg gcactcgcgg
    10981 gtactcccag ttcttttaaa tatttaaaga ttcccttcgc cgtgctttca ttcatcgaac
    11041 ttggtgttgg cgctgataca tccatggcac caatgaaatc cagggcttta tctattacag
    11101 caggcatatt cttatcctta aaattaacca tgttatcgat aaggctatat atgaccttaa
    11161 tttgcatcta atatcaaagg gattataacg atatggcacg agttaacctg ttgacattca
    11221 ggtgttttaa aggtagcagc tcatggtgtg ttatccagta agatggagaa aattaacggt
    11281 cagcccgcac gctataacca gattgttcag ctaagaaaga aaaccagagt tgctaatcaa
    11341 agggcgaaca caacggcatg gttatgttta tttatatagt tagttttata gataaaattg
    11401 aaattataaa tataaataat gaattagcag aagcatcaac agtaatatct tattctctgg
    11461 tcgatttatg taaaaccgac cagtattatc gataaattat cgtctgactt attttgcttc
    11521 gataaagctt acaccgcagc cacaatctta atttcaactt tatacttcgg gttcatcaaa
    11581 cccgcttgta ccgtgcagcg caccggcgca tgacccgcga caacccaggc atcccacgct
    11641 ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa tggtggcatc cagaatgctc
    11701 gatttattgc tgccctgttt ttccagcacg gcgtcaatct gtgccagcgt gttggcggtt
    11761 tgctcaaagg catcggcgtc gaggttttcc ggtacaccag tgtagtagag cgtgttgttg
    11821 tggattacta catcagacca gcgggcttca gcatcgatac gaacgatagt catcattttg
    11881 tcctcattat acttccattt tcgtgctgca aagactgcca caatgtgagg ttgccgtcac
    11941 cacttcaact ggcgaaagcg ccctcatcct gacataatcc ctgttcaaat caacaggggg
    12001 tagtgtgacg gacgattttg caccagacgg tcagctggcg aaagcgatac caggctttaa
    12061 gccgcgagaa ccacagcgac agatggcggt agccgtcacc caggcgatag aaaaaggcca
    12121 gccgctggtg gtggaagcag gaaccggtac gggcaaaacc tacgcttacc tggctcctgc
    12181 gctgcgggcg aaaaagaaag tcattatctc gaccggctca aaagcgttgc aggatcagct
    12241 ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa tatacgggca acgtggcgct
    12301 gctgaaaggg cgctcaaact acctctgcct cgaacgtctc gaacagcagg cgctggcggg
    12361 gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg ctgcgctcct ggtctaatca
    12421 aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg gaagattcac aggcgtggcc
    12481 gctggtcacc agcaccaacg acaactgtct tggcagcgac tgcccgatgt ataaagattg
    12541 ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat gtggtggtgg taaaccatca
    12601 tctctttctg gcggatatgg tggttaaaga gagtggattt ggcgagttga tccctgaagc
    12661 ggacgtcatg atcttcgacg aagcccacca gctaccggac attgccagcc agtattttgg
    12721 tcagtcactc tccagtcgac aactgctcga cctggcaaaa gacatcacca tcgcctaccg
    12781 caccgaatta aaagacaccc agcagttaca aaagtgcgct gatcgtcttg cccagagtgc
    12841 gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc ggtaacctgc gtgagctgtt
    12901 agctaatccg caaattcagc gggcattttt actgctcgat gacaccttgg aactttgtta
    12961 tgatgtggcg aaactgtcac tggggcgttc cgccttgctg gatgcggcat ttgagcgcgc
    13021 cacgttgtat cgcacacggc tgaagcggct aaaagagatc aatcagccgg gctacagcta
    13081 ctggtacgaa tgcacttcgc gccattttac tctggctctc acgccgctca gcgtggcgga
    13141 taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg atcttcacct cagcaacgct
    13201 gtcggtgaac gacgatctgc atcatttcac ctcgcggctt gggatagaac aggcggagtc
    13261 gttgctattg ccaagcccgt ttgattacag ccgccaggcg ttactctgtg tgccgcgcaa
    13321 tctgccgcaa accaaccagc caggttctgc tcgccagtta gcggcaatgc tgcgaccgat
    13381 catcgaagct aacaacggtc gttgttttat gctttgtacc tcgcacgcca tgatgcgcga
    13441 tctggccgag cagttccgcg ctaccatgac gcttcccgtt ttgttgcagg gggaaaccag
    13501 taaaggccaa ctgttgcagc agtttgtcag tgccggtaat gcgcttcttg tggcaaccag
    13561 tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg tcattggtaa ttatcgacaa
    13621 attgccgttt acctcgccgg atgatccact gttaaaagcg cgcatggaag attgtcgttt
    13681 gcgcggtggc gacccgttcg atgaagtgca actaccagat gccgtcatta ctctcaaaca
    13741 gggggtaggt cgactgattc gcgacgccga cgatcgtggc gtgctggtga tttgtgacta
    13801 tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc agtctgccgc ccgcgccacg
    13861 cacccgtgac attgcccgtg cggttcgttt ccttgcgata ccatcctcca ggtaatttgt
    13921 tacggagtat ggtatgatgc gcgccttttt attgatctaa gcacgaagac ccatgcgaat
    13981 tctggctatc gataccgcga cagaggcctg ctctgtcgcc ctgtggaacg acggtactgt
    14041 caacgctcat tttgagcttt gccctcgtga acatactcaa cgaatcttac cgatggtgca
    14101 ggatatcctg accaccagcg gaacttccct gactgatatt aacgctctgg cttacgggcg
    14161 cggccccggt agctttaccg gcgtgcgcat tggtattggc atcgcgcaag ggctggcgct
    14221 tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg acgatggcgc aaggggcgtg
    14281 gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac gcgcgaatgg gcgaagttta
    14341 ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac ggtgaagaaa ccgaagccgt
    14401 actcaaaccc gaactcgttc atgaacgaat gcaacagctt tccggcgaat gggtgacggt
    14461 agggacgggc tggcaagcct ggccggatct cggtaaagag agcgggctgg ttttgcgcga
    14521 tggcgaagtg ttactccccg ctgcagaaga tatgctgccg attgcttgtc agatgtttgc
    14581 cgagggtaaa acggtggcgg tggaacatgc cgagccggtt tatttgcgta acaacgtcgc
    14641 atggaagaaa cttccgggca aagaatgaat cttaagagta tgccctgaga aaaaaaggag
    14701 tcgcatcatg gcggttcaaa agaatgttat caaaggcata ctggcaggta cgtttgcgct
    14761 aatgctgagc ggttgtgtca ctgtgccgga cgccattaaa ggcagcagtc ccacgccgca
    14821 acaagattta gttcgggtga tgagtgcgcc gcagctgtac gttggtcagg aggcacgctt
    14881 tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa acccgcctgg aaattgctac
    14941 cgtaccgctg gacagcggag ctaggccgac gctgggagaa ccttctcgcg gtcgcattta
    15001 tgccgatgtg aacggttttc tggacccggt ggatttccgt ggacaactgg ttacggtagt
    15061 cgggccaatc accggtgcgg ttgacggcaa aatcggcaat acgccctata aatttatggt
    15121 gatgcaagta acgggttaca aacgttggca tttaacccag caggtgatta tgccgcctca
    15181 gccgattgat ccatggtttt atggcggtcg tggctggccc tatggctacg gcggatgggg
    15241 ctggtataat cccggccccg cgagagtaca aacagttgta accgaataat tgcttgtttt
    15301 taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacagtgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acgctctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgtcta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggggatacca ataccgcccg cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgagca tagctcttga attgagcatt tttctgcggt
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DBKELA010000068        21253 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.068, whole genome shotgun sequence.
ACCESSION   DBKELA010000068 DBKELA010000000
VERSION     DBKELA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.068"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..791
                     /locus_tag="ACUQR0_002556"
     CDS             <1..791
                     /locus_tag="ACUQR0_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HHV9109543.1"
                     /translation="AAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQI
                     AVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEKV
                     TDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTDH
                     YYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFSF
                     FFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="ACUQR0_002557"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="ACUQR0_002557"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HHV9109544.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="ACUQR0_002558"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="ACUQR0_002558"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HHV9109545.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="ACUQR0_002559"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="ACUQR0_002559"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HHV9109546.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /gene="relA"
                     /locus_tag="ACUQR0_002560"
     CDS             complement(4989..7223)
                     /gene="relA"
                     /locus_tag="ACUQR0_002560"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HHV9109547.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="ACUQR0_002561"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="ACUQR0_002561"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HHV9109548.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="ACUQR0_002562"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="ACUQR0_002562"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HHV9109549.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="ACUQR0_002563"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="ACUQR0_002563"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HHV9109550.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="ACUQR0_002564"
     CDS             complement(12978..14318)
                     /locus_tag="ACUQR0_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HHV9109551.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="ACUQR0_002565"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="ACUQR0_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HHV9109552.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="ACUQR0_002566"
     CDS             complement(16106..16555)
                     /locus_tag="ACUQR0_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HHV9109553.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="ACUQR0_002567"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="ACUQR0_002567"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HHV9109554.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="ACUQR0_002568"
     CDS             complement(17355..17684)
                     /locus_tag="ACUQR0_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HHV9109555.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="ACUQR0_002569"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="ACUQR0_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HHV9109556.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="ACUQR0_002570"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="ACUQR0_002570"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HHV9109557.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="ACUQR0_002571"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="ACUQR0_002571"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HHV9109558.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5670 c         5071 g         5286 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctggtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat gccgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatatcg
    16861 gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagatatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaatgc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccgc tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DBKELA010000069        20975 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.069, whole genome shotgun sequence.
ACCESSION   DBKELA010000069 DBKELA010000000
VERSION     DBKELA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.069"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1543
                     /locus_tag="ACUQR0_002572"
     CDS             <1..1543
                     /locus_tag="ACUQR0_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HHV9109559.1"
                     /translation="TPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVV
                     DTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFG
                     VYSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNF
                     TTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGV
                     VDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVK
                     RILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWK
                     GKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTW
                     ITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVE
                     NNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKA
                     LSEAK"
     gene            1595..2752
                     /locus_tag="ACUQR0_002573"
     CDS             1595..2752
                     /locus_tag="ACUQR0_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HHV9109560.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2718..2840)
                     /locus_tag="ACUQR0_002574"
     CDS             complement(2718..2840)
                     /locus_tag="ACUQR0_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109561.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3043..4728
                     /locus_tag="ACUQR0_002575"
                     /pseudo
     CDS             3043..4728
                     /locus_tag="ACUQR0_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4766..7126
                     /locus_tag="ACUQR0_002576"
     CDS             4766..7126
                     /locus_tag="ACUQR0_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HHV9109562.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7183..9966
                     /locus_tag="ACUQR0_002577"
     CDS             7183..9966
                     /locus_tag="ACUQR0_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HHV9109563.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10327..11727
                     /locus_tag="ACUQR0_002578"
     CDS             10327..11727
                     /locus_tag="ACUQR0_002578"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - gamma-aminobutyrate shunt
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HHV9109564.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            11883..13418
                     /gene="gadC"
                     /locus_tag="ACUQR0_002579"
     CDS             11883..13418
                     /gene="gadC"
                     /locus_tag="ACUQR0_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HHV9109565.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13549..14868
                     /locus_tag="ACUQR0_002580"
     CDS             13549..14868
                     /locus_tag="ACUQR0_002580"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HHV9109566.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15245..16315
                     /gene="dosC"
                     /locus_tag="ACUQR0_002581"
                     /pseudo
     CDS             15245..16315
                     /gene="dosC"
                     /locus_tag="ACUQR0_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            16315..16932
                     /locus_tag="ACUQR0_002582"
                     /pseudo
     CDS             16315..16932
                     /locus_tag="ACUQR0_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            17190..17522
                     /gene="ddpX"
                     /locus_tag="ACUQR0_002583"
                     /pseudo
     CDS             17190..17522
                     /gene="ddpX"
                     /locus_tag="ACUQR0_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            17536..18432
                     /gene="ddpC"
                     /locus_tag="ACUQR0_002584"
     CDS             17536..18432
                     /gene="ddpC"
                     /locus_tag="ACUQR0_002584"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HHV9109567.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18429..19415
                     /locus_tag="ACUQR0_002585"
     CDS             18429..19415
                     /locus_tag="ACUQR0_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9109568.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            19408..20334
                     /locus_tag="ACUQR0_002586"
                     /pseudo
     CDS             19408..20334
                     /locus_tag="ACUQR0_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(20390..20821)
                     /gene="osmC"
                     /locus_tag="ACUQR0_002587"
     CDS             complement(20390..20821)
                     /gene="osmC"
                     /locus_tag="ACUQR0_002587"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HHV9109569.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         5642 a         4906 c         4929 g         5498 t
ORIGIN      
        1 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
       61 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      121 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      181 atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta acggctatgt
      241 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      301 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      361 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      421 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      481 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      541 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      601 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      661 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
      721 accaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
      781 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
      841 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
      901 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
      961 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1021 taattatgac aagctgattt ccgcaatgga tttctaccca acagctcttg atgcagccga
     1081 tatcagcatt ccaaaagacc ttaagctgga tggtgttccc ttgctgccct ggttgcaaga
     1141 taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt
     1201 tgatgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga
     1261 cgattacccg cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa
     1321 taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac
     1381 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1441 aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgagg taaatcagga
     1501 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc
     1561 ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1621 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1681 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgaagc
     1741 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     1801 ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa
     1861 tgcattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca
     1921 tgaatttctg gttggtatct cgatcgatgg cccccaggag ttacatgacc gttacagacg
     1981 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2041 atatcaagta gagtttaata cgttaaccgt cattaataac gttaatgtcc attatcctct
     2101 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2161 agaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2221 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2281 ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt
     2341 tttggggaat gggcatacaa gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2401 tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg
     2461 aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2521 acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc
     2581 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     2641 aatccttttt tcaaccatgg taccttatat gaacgccatg gttgagttag ctaagaacag
     2701 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     2761 tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     2821 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     2881 cgtcagggca ttagcaatac ccttgattat caatagcata accataacca ctgaaaatta
     2941 aaaatttcta tatatttcat attgttaaat gatgcttaaa taaatttcat cagtaaatca
     3001 ttgccataga taatcattgg atggcacaat caattttcgc taatgataac cattcccatt
     3061 acgttttgta tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag
     3121 aataataaaa cttcagtgtt gttaatcatt attatcctcg caatgatcct tggcgtggtt
     3181 aaaattcagg tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa
     3241 gaaaccgata agctttggca gctcgttctt tggttccctg ccctgctggg gatctttgta
     3301 ttaatatccg taaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg
     3361 ttaacagatt actaccttaa tcgctggttt gcagataaaa actactactt cacgcagatc
     3421 tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta
     3481 ctgatcaaca aaacgctcag tctttcattt ggcttcatcc agtcacttag catgctgatc
     3541 acctttaccg ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca
     3601 gaatggaata tccaggggta tatggtctat accgtcgtgc tcattgtgat cggtggaact
     3661 ttatttaccc ataaggtggg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc
     3721 gaagcgacat ttcgaactaa tcttgtgcag cataataagc aggcagaact gattgcgcta
     3781 agcaatgctg agtctctaca acgccaggag ttgcgcgaca attttcatac cattaaagag
     3841 aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcaaaa tatttattcg
     3901 cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc
     3961 aatctgggcg gactgatgaa atcgcgccag gcatttatgc tagtatcgaa caatttaagc
     4021 tggtttattt ataaatatga cgaacttgct gaactggctg cggttatcga tcgcttgtat
     4081 gagttccatc aactcactga atagcgccct acgaataagc ctaaaaattg ccaacatgcg
     4141 gtacaagtgg ctaatgcgag tattcgtacg cctgataata agatcatatt agagaacttg
     4201 aactttcatg tttcgccagg caaatggcta ttactgaaag gctactctgg tgcgggaaaa
     4261 accacactgc ttaaaacatt atcccactgc tggccgtggt ttaaaggtga tatttcttct
     4321 cctgctgaca gttggtatgt gtcacaaaca ccgttaatca aaagcggctt actgaaagag
     4381 attatttgta aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat
     4441 caggttggtc tgggaaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt
     4501 ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa
     4561 tggatatttc ttgacgaaac tacctctcat cttgaggaac aagaggctat acgcttactg
     4621 cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaacccggt
     4681 gtctggaacc tggctgatga tatttgtgac attagcacgg ttttataaca acaataaaag
     4741 ccctgctgta cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg
     4801 tggcgctgat gtggcgcagg ccgtcgatga caaaaacatg tacatgtatt tttttgaaga
     4861 gatgacggtc tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga
     4921 aagcatcgag cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc
     4981 tgcggtacgc atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgccctga
     5041 gaaaatctct attcacggtg cgtcgcccta ccagaatgcc tatttgattg acggtattag
     5101 tgcaactaat aacctgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag
     5161 cgggatgtca caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga
     5221 cagttttgtg ccggttgaat ttggtcgctt caatggcggg gtaattgatg caaagatcaa
     5281 acgcttcaac gctgatgata gcaaggtgaa attgggttat cgcactacgc gtttggactg
     5341 gttaacatcg catatcgatg agaataacaa gagcgcattt aatcaaggtt cttcaggaag
     5401 tacctatttc tctccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggagct
     5461 ggctgataac tttggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc
     5521 ggattatgtt tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga
     5581 tactgcattg agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt
     5641 aaaatatacc ggctccagcc gtgattataa taccagcacc ttcccgcagt ctgatcgcga
     5701 aatgggtaat aaatcctatg gtctggcatg ggatatggat acgcaactcg catgggccaa
     5761 actacgtacc accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat
     5821 ctggtacacc gaactttcat gtacatatgg tgatattaca gggcgttgta cccgtggcgg
     5881 attaggacac atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca
     5941 aaaattcgcc gtgggtgatg tttcgcatca accctacttc ggcgcggaat acatctattc
     6001 cgatgcatgg actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa
     6061 gaaaactaac cataccattt accataaagg taaaggcagc ctgggaattg acaactacac
     6121 gctgtatatg gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta
     6181 tgactatgac aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga
     6241 tatttttgct gatcaaacct caatgattac cgccggttat aaccgttact atggcgggaa
     6301 tattcttgat atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggtaa
     6361 taaaaccctg acgcgttatc agaatttgaa aacgccttat aacgatgaac tggcaatggg
     6421 attgcagcaa aaaatcgata agaacgttat tgcacgcgca agtgaagcgc atgatcaaat
     6481 cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc attactgaat ataacaacga
     6541 tggcaaaacc aaaacgcatt cgtttaacct cagttttgaa ctggccgaac ccctgcatat
     6601 ccgccaggta gatattaacc cgcaaattgt ctttagctat atcaagagca agggcaactt
     6661 gtcgttaaac aatggttatg aggagagcaa taccggtgat aaccaggtgg tttataacgg
     6721 taatctggtc tcttacgata gcgttccagt ggcagatttt aataacccat taaagatctc
     6781 cttaaacatg gatttcacgc atcaaccgag cgggttagtg tgggcgaata cgctggcctg
     6841 gcaagaagcg cgtaaagctc gcattatcct tggtaagaca aatgcgcaat acatcagcga
     6901 atattcagat tacaagcagt atgttgacga aaaactggat agcagcctga cctgggacac
     6961 ccgcttgtcc tggacgccac aatttctgaa acaacaaaac ctgacgatca gtgccgatat
     7021 tctcaatgta ctggatagca aaaccgctgt tgatacaacg aacaccggtg tggcgaccta
     7081 cgccagtggc cgtactttct ggcttgatgt cagcatgaaa ttttaatagt tcggatttta
     7141 cttaaccggg taaattcgcc cggttttcgc atggagataa ttatgagaaa cctctgtttc
     7201 ttactgacgt tagtggcaac tctgttgctc cccgggcggc tgattgccgc cgccttaccg
     7261 caggatgaaa agttaattac cgggcaactg gacaatgact tgcgatatat gatttatccg
     7321 catgctcatc caaaggatca ggtaaattta tggctgcaaa ttcataccgg ttcattgcag
     7381 gaagaagaca atgagctcgg cgtggctcat tttgtagaac atatgatgtt taacggcaca
     7441 aaaacatggc cgggtaataa agtcatcgaa acatttgagt caatgggcct gcgttttggt
     7501 cgcgatgtta atgcctatat cagctatgac gaaacggtgt atcaggtgag tttgccgact
     7561 acgcagaaac aaaatctgca acaagtgatg gcaatcttca gtgaatggag taatgccgca
     7621 acctttgaaa aactcgaagt agacgctgaa cgtggcgtaa ttactgagga atggcgtgcc
     7681 catcaggatg cgaaatggcg cacctctcag gcgcgccgcc ctttcctgct ggctaatacc
     7741 cgtaatttag accgtgaacc tatcggcctg atggatactg tcgccacggt cacgccggca
     7801 caattgcgcc aattttatca acgctggtat caaccaaata atatgacctt tatcgtggtc
     7861 ggcgatatcg acagtaaaga agcgctggcg ctgataaagg ataatttaag taagcttccg
     7921 gctaacaaag cagctgaaaa tcgcgtctgg ccgacaaaag ccgaaaacca cctgcgcttt
     7981 aatatcatta atgataaaga aaaccgggtg aacggcatcg cactctatta tcgcctgcca
     8041 atggtacaag tgaacgatga gcaaagcttt gtcgaacaag ctgaatggag catgttagtt
     8101 cagctgttca atcaacgtct gcaggaacgc atacagtcgg gcgagttgaa gactatttct
     8161 ggcggcactg cgcgcagcgt taaaattgca cccgattatc agtcgctgtt tttccgtgta
     8221 aatgcacgag acgataatat gcaggatgct gcgaatgcat taatggcaga gttggcaacc
     8281 attgatcagc atggtttttc tgctgaagaa ctcgatgatg tcaaatctac ccgcctcacc
     8341 tggctgaaaa atgcggttga tcagcaagct gaacgtgatt tacgtatgct gaccagtcgc
     8401 ctggcatcca gctcattaaa taatacgccg ttcttgtcgc cggaagagac atatcaactt
     8461 tcgaaacgtc tgtggcagca aattaccgtg caaagtctgg cggaaaaatg gcagcagtta
     8521 agaaagaacc aggacgcatt ttgggagcaa atggtaaaca atgagcttgc cgccaaaaaa
     8581 gcattgtctc ctgcggctat cctggcgctg gaaaaagagt acgccaacaa aaagctggcg
     8641 gcttacatct tcccaggcag aaatttatcg ttaacagtag acgctgaccc acaggcggaa
     8701 attagcagca aagaaacgct ggctgagaat ctgacatcat taacactttc caatggtgcc
     8761 aaggttattc tggcaaaatc cgcgggtgaa gagcaaaagc tacaaattat tgccgtatcg
     8821 aataaaggcg atttaagttt ccctgcgcag caaaaatcac ttatcgcgct ggcaaataaa
     8881 gcagttagcg gaagcggcgt tggcgaactc tcctcttcca gcctgaaacg ctggagtgcg
     8941 gaaaactcgg taaccatgag cagtaaagtc agtggcatga atacgttgct ctccgttagc
     9001 gcgcggacta ataaccctga acctggtttc cagttgatta accagcgaat cacccacagc
     9061 acgattaacg acaatatttg ggcatcgcta caaaatgctc aaattcaggc gttgaaaacg
     9121 ctcgaccagc gtccagcgga gaaattcgcc cagctgatgt atgagacgcg ctatgctgat
     9181 gaccgcacga aattactgca agaaaatcag attgcacagt ttactgccgc agatgcgctg
     9241 gctgccgatc gccagttgtt ttcatctcca gcggatatca cgtttgtcat tgtcggtaat
     9301 gtcgcagaag acaaactcgt ggcgttaatt acgcgttact taggctcaat caaacactct
     9361 gattcgccat tagccgcagg taaaccatta actcgcgcga cggacaacgc atcggttact
     9421 gtaaaagaac aaaatgaacc tgtggcacag gtttcacagt ggaagcgtta tgattcccgg
     9481 acacctgtta atctggcgac gcgtatggcg ctcgatgctt ttaacgtcgc actggcaaaa
     9541 gatctacgcg ttaatattcg tgaacaggca tctggggcat acagcgtttc ttctcgcctc
     9601 tcggttgatc ctcaggctaa agatatcagt catttgctgg cttttacttg tcaaccagaa
     9661 cgacatgatg aacagttaac gttagcgaat gaagtgatgg ttaagcgttt gactaagggg
     9721 atcagtgagc aagaactgaa cgaataccag caaaacgttc agcgcagcct cgatatccaa
     9781 cagcgtagcg ttcaacaatt agcgaacact attataaata gtcttattca atatgacgat
     9841 cctgcagcat ggactgagca ggagcaattg ttgaaacaaa tgacggtaga gaatgttaac
     9901 actgccgtta aacaatatct ttctcatccg gtaaatactt ataccggagt attattgcca
     9961 aaataataac agccccgtca acacatcgtt ggcggggatt ttagcaatat tcgctatttt
    10021 tatgtaataa ttttataaat gcgttcaaaa taataaccaa gtactaatag tgatatttta
    10081 atgcctgatt tttacgtgat aattcaggag gcacagaaag cgcataaaaa taacaacata
    10141 aaacaccttt ccaccaccca agaattctct attacattgt ttttaatgaa aaaatatcat
    10201 tccaacaata tctcacgata aataacatta ggattttgtt atttaaacac gagtcctttg
    10261 cacttgctta ctttatcgat aaatcctact tttttaatgc gatccaatca ttttaaggag
    10321 tttaaaatgg ataagaagca agtaacggat ttaaggtcgg aactactcga ttcacgtttt
    10381 ggtgcgaagt ctatttccac tatcgcagaa tcaaaacgtt ttccgctgca cgaaatgcgc
    10441 gacgatgtcg catttcagat tatcaatgat gaattatatc ttgatggcaa cgctcgtcag
    10501 aacctggcca ctttctgcca gacctgggac gacgaaaacg tccacaagtt gatggattta
    10561 tccattaaca aaaactggat cgacaaagaa gaatatccgc aatccgcagc catcgacctg
    10621 cgttgcgtaa acatggttgc cgatctgtgg catgcgcctg cgccgaaaaa tggtcaggcc
    10681 gttggcacca acaccattgg ttcttccgag gcctgtatgc tcggcgggat ggcgatgaaa
    10741 tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa cggataaacc aaacctggtg
    10801 tgcggtccgg tgcaaatctg ctggcataaa ttcgcccgct actgggatgt ggagctgcgt
    10861 gagatcccta tgcgccccgg tcagttgttt atggacccga aacgcatgat tgaagcctgc
    10921 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
    10981 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
    11041 gacatgcaca tcgacgccgc cagcggtggc tttctggcac cgttcgtcgc cccggatatc
    11101 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
    11161 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
    11221 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
    11281 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
    11341 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
    11401 aaactggggc cgtatgagtt catctgtacc ggtcgcccgg acgaaggcat cccggcggtt
    11461 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
    11521 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
    11581 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
    11641 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
    11701 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
    11761 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
    11821 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
    11881 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggattctttg
    11941 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
    12001 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
    12061 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
    12121 caaatactct gggtccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
    12181 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
    12241 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
    12301 cgctggcatt aacgcagttt ggtggcacga aatacacggc gcgaattgct aaagttggct
    12361 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttacctgc
    12421 actccggtgc ccccgttgct atcgaaatgg attcgaagac cttcttccct gacttctcta
    12481 aagtgggcac actggttgta tttgttgcct tcattttgag ttatatgggc gtagaagctt
    12541 ccgcaactca cgtcaatgaa atgagcaacc ccgggcgcga ctatccactg gctatgttac
    12601 tgctgatggt ggcggcaatc tgcttaagct ctgttggcgg tttgtctatt gcgatggtca
    12661 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
    12721 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
    12781 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
    12841 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
    12901 cgctggtcat ttcgcagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
    12961 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
    13021 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
    13081 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggca attgtcggtc
    13141 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
    13201 attctaccga tatgtatgtt gaattactgg ttgttagttt cctggtggta cttgccctgc
    13261 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
    13321 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
    13381 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
    13441 caagggagcg ataattcatc gctccctttt tcgtgcttgc tgtcgtattg acttaaccgg
    13501 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcacatat ggatatctgc
    13561 tcccgaaaca agaaattaac gattagaaga ccagcgatac tggttgcatt ggcactttta
    13621 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
    13681 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtt
    13741 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaatcccac cagccgagcc
    13801 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tccaacgtct cggcataaac
    13861 acggtctttt tccaggtcaa gccggacggt accgccctgt ggccatcgaa aattttgccg
    13921 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattc
    13981 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
    14041 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
    14101 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
    14161 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc tgaagtggtt
    14221 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga atcaccgggt
    14281 tcacggctaa atgataacga aacgtaccgt aaatacggag gcgcatttgc gtcaaaagca
    14341 gactggcggc gcaacaatac tcagcagtta attgcaaagg tatcacacac cattaaaagc
    14401 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
    14461 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgcagacacc
    14521 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
    14581 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaacca
    14641 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
    14701 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
    14761 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
    14821 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac taccatgctt
    14881 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
    14941 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
    15001 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
    15061 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtctt
    15121 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
    15181 gatctcgcac gttgcgtttc gttttgattc cgtctgattt cccagcctta taagggtgtg
    15241 aattatggag atgtatttta aaagaatgaa agatgagtgg accggacttg tcgaacaggc
    15301 agatcctctc attcgtgcta aagccgcaga aattgccctt gcgcatgctc attatctgag
    15361 tatcgagttt tatcgaattg tccgcatcga cccgcatgcc gaagaattct tgagtaatga
    15421 acaagttgag cggcagttga aaagtgcgat ggaacgctgg attattaacg tgctttctgc
    15481 ccaggttgac gatgtcgaaa ggctaataca aatccagcat accgtcgcgg aagtgcatgc
    15541 ccgcatagga attccggtag aaattgtcga gatggggttt cgggtgctga aaaaaatcct
    15601 ctatccggta atcttctctt cggattattc cgccgcagaa aaacttcagg tctaccattt
    15661 ctcgattaac agtattgata tcgcgatgga agtgatgacc cgcgcgttta cctttagtga
    15721 cagtagtgcc tcaaaggaag atgaaaacta tcgtatcttc tcgttactgg aaaacgccga
    15781 agaagaaaaa gaacggcaaa tagcctcaat actttcatgg gaaatagata ttatctataa
    15841 agtcctgctg gattctgatt taggcagcag tttgccttta agccaggctg attttggcct
    15901 gtggtttaac cataaaggtc gacattattt tagcggcatt gccgaagtgg gccatatctc
    15961 ccgtctgatt caggatttcg acggtatttt caatcaaacc atgcgtaaca ccaggatttt
    16021 gaataacaga agtttgcggg taaaatttct attacagata agaaataccg tatcgcagat
    16081 tattaccttg ctgcgtgaat tgtttgaaga agtatcgcgc cacgaagtcg gtatggatgt
    16141 actgacgaaa ttacttaacc gccgtttcct gccgactatc ttcaaacgcg aaattgccca
    16201 tgccaaccgg accggtacac cgctgtcagt gctgattatt gacgttgata aattcaaaga
    16261 gatcaacgat acgtggggcc ataacactgg cgatgaaatt ctgcgtaaag tctcattcct
    16321 ctcgcagaag agattggtga aatcgaaaat attgggcgct gggtcatcgc ggaagcttgc
    16381 cgtcagttag cagaatggcg tagccagaat attcatatcc cggcgttatc cgtgaacttg
    16441 tcggcgctgc actttcgcag taatcagctg cctaatcagg tgtctgatgc aatgcaagcc
    16501 tggggtattg acggccacca gctgacggtg gaaatcacgg aaagcatgat gatggaacac
    16561 gataccgaaa tctttaagcg cattcagatc ctgcgtgata tgggcgtggg tttatcggta
    16621 gatgattttg gcacgggctt ttccggatta tcccgcttag tcagtcttcc ggtaacggaa
    16681 atcaaaattg acaaaagctt tgtcgatcgt tgtctgacgg aaaaacgcat cctggcctta
    16741 cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa ccgtcgtggc ggaaggggtc
    16801 gaaaccaaag agcaatttga gatgctacgt aagatccact gtcgcgttat tcagggatat
    16861 ttcttttccc gcccccttcc cgccgaagaa attccaggct ggatgagcag cgtgttaccg
    16921 ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt tttcctttaa ggtgacactg
    16981 tcacttaaat actgtgattt cagctacgat tccgggagat tccttcctta actttccctc
    17041 ttgcaccaaa actgcactac gctgcacaaa tgttgatcaa aagttaaaaa atataacttt
    17101 aagccagcat tctgccgctt atcccggcat ggcatgagat ctgcataagc ggaaagcgca
    17161 gcaatttttg tcttatacag gaaccgttta tgtcggatac caccgaactg gttgatttag
    17221 ccgtgatctt ccctgatctg gagatcgaat tgaaatatgc ctgcgttgat aacatcacag
    17281 gtaaagctat ttatcagcaa gcgcgttgtc tgctacataa agatgcgatt accgcgctgg
    17341 cgaaaagtat cagtatcgcc cagctgtcag gcttacaact ggtgatttac gatgcgtatc
    17401 gcccacaaca agcacaggcg atgttgtggc aagcctgccc agacccgcaa tatgttgttg
    17461 atgtgacggt cggttctaat cacagccgtg gcacggcgct cgacctgacg cttcgtgatg
    17521 acgtggaggt ggtgaatgat gctaagcgag gaaacgtccg ccgtacgccc acaaaaacaa
    17581 acgcgattta acggtgcaaa actggtatgg atgctgaaag gcagtccgct caccgtgacc
    17641 ggcgcagtca tcattgtatt aatgcgactg atgatgattt tttcaccgtg gctggcgacg
    17701 catgatccca acgccattga tttaaccgcc cgccttttgc cgccttctgc ggcgcactgg
    17761 tttggcaccg atgaagtggg acgcgatctg tttagccgcg tactggtcgg cagtcagcaa
    17821 tcaattctcg ccggattagt ggtggtcgcc attgcgggta tgattggttc gctactcgga
    17881 tgtctatccg gtgtgcttgg cggacgcgca gacgccatca tcatgcgcat catggacatt
    17941 atgctgtcga ttccttcgtt ggtactgaca atggcactgg ctgccgctct cgggccgagt
    18001 ttatttaacg ccatgctggc gattgctatt gtgcgaattc ccttttatgt gcgcttggcg
    18061 cgggggcaag cattagttgt acgccagtat acctatgttc aggcggcgaa aacctttggc
    18121 gcgtctcgtt ggcatctgat caactggcat attttacgta actccctacc gccgctgatc
    18181 gtgcaggcat cgctggatat cggtagcgcg attttaatgg ccgccacgtt gggatttatt
    18241 ggcctaggtg ctcaacaacc gagtgctgaa tggggcgcga tggtagcgaa tggtcgcaac
    18301 tatgtactcg atcagtggtg gtattgcgca tttccggggg cagcgatttt gcttacctcc
    18361 gtcgggttta atctctttgg tgatggtatt cgcgatctgc ttgacccgaa agcaggagga
    18421 aagcagtcat gacccaaccc gttctggaca ttcaacagct gcatttgagt ttccccggtt
    18481 ttaacggtga tgttcacgcg ctcaacaatg tgtccttgca gattaaccgc ggcgaaattg
    18541 tcggtctggt gggagaatcc ggctcaggta aatcagtcac cgcaatgctg attatgcgtc
    18601 tgctaccgac gggcagttat tgcgtacatc gggggcagat ttcactgctg ggagaagatg
    18661 ttcttaacgc tcgggaaaaa cagcttcgtc agtggcgcgg cgcacgagtg gcgatgatct
    18721 ttcaggaacc gatgaccgcc cttaatccga cacgtcgaat aggtcttcag atgatggacg
    18781 taatccgcca tcatcaacca ataagtcgtc gggaagccag agctaaagcg attgccctgc
    18841 tggaagagat gcaaatcccg gatgccgtgg aagttatgtc gcgctatccg tttgagcttt
    18901 caggtggtat gcgccagcgg gtaatgattg cgctggcatt ctcctgcgag ccgcaattga
    18961 ttattgccgg cgaaccgacg acggcgctgg acgtcacagt acagttgcag gtactgcgtt
    19021 tgcttaaaca taaagcacgc gccagtggaa ctgcggtact gttcatcagc catgatatgg
    19081 ccgtggtgtc gcaactgtgc gatagcgttt acgtgatgta tgccggaagc gtaattgaaa
    19141 gcggcgggac ggcagacgtt atccatcatc cccggcatcc gtataccatt ggtttgctgc
    19201 aatgcgcacc agaacatgga gtaccacgcc agccattacc cgccattcca gggacggtac
    19261 caaacctcac ccatttgcct gacggctgcg cttttcgcga tcgttgctat gcggcaggtg
    19321 cacagtgtga aaacgtcccg gcgctgacag cgtgtggtga caacaaccag cactgcgcct
    19381 gttggtatcc gcagcaggag gtcataagtg tctgacacgt tattaacgtt acgcgacgtc
    19441 catatcaatt ttccggcccg taaaaactgg cttggtaaat ctacggaaca tgttcatgcc
    19501 attaatggta ttgatttaca gatccgccgt ggtgaaacct tagggatcgt cggcgagtca
    19561 ggctgcggca aaagcaccct cgcacagctt ttaatgggca tgctgcaacc gagccacggg
    19621 cagtacatcc gttcaggctc acaacgcatt atgcagatgg tgtttcagga cccgctctct
    19681 tcgcttaatc cgcgcttacc ggtgtggcgc atcatcacag aatcgctctg gatagctaag
    19741 cgtagtagtg aacaacagcg gcgagcgttg gcagaggagt tggctgtgca ggtgggtatt
    19801 cgtccggagt atctcgaccg cctgcctcat gcgttctccg gcgggcagcg gcaacgcatc
    19861 gccattgcca gagcactctc ttcgcagcct gacgtgattg tgcttgatga gccaacctcg
    19921 gcgctggata tctccgtgca ggcgcagatc ctcaatttgc tggtaacgct acaggaaaat
    19981 cacgggctga cttatgtgct gatttcacac aatgtctcgg tgatacgtca tatgagcgat
    20041 cgggtggcgg tgatgtatct cgggcagatt gtagaactgg gcgacgcgca acaggtgttg
    20101 actgcttcgg cacatccata cacccgatta ttgctggatt ccctccccgc cattgataaa
    20161 ccgctggagg aagaatgagc attacgtaaa acggatctgc cgggaaaccg cacgttgccg
    20221 caaggctgtt ttttctacga acgttgccct ctggcaaccc acggatgtga agtccggcaa
    20281 tcattagcaa taagggagga cggacgtgag ctccggtgct ggcgggcgct gtagagatgc
    20341 taacaacgca tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt tacgatttca
    20401 actggtaatc cagcgtaatt tccgctttca gtacctgaga gaccgggcat cctgcttttg
    20461 ctttctggat gatgccgtca aaggtagagg catcaatacc cggcaccgca acttcactct
    20521 tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt atccagcgac acatcggcgg
    20581 tggtatcaat tgatgttggc gtgaatcccg cttcccccag cattaatgaa agcgccattg
    20641 agaaacatgc ggcatgcgct gcgccaatca gttcttcagg gttggttcct ttttcgcctt
    20701 caaaacgcgt gttaaatcca tacggctgtt ggttcagcac gccgctctcg gtggatactg
    20761 ttcccttccc gcgtttgata tcgccttccc agtgtgcctg acctttctta tggattgtca
    20821 ttgttgctct cctgtgggct taaaaaggaa attatagact acgtgagaaa cagcacgagg
    20881 ataagcagga tattccgaat aaaatacggc gaaaatatta cttctgcact taaattaaat
    20941 tcccacaata gataaccggg aatatatatc tcaag
//
LOCUS       DBKELA010000070        20714 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.070, whole genome shotgun sequence.
ACCESSION   DBKELA010000070 DBKELA010000000
VERSION     DBKELA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.070"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..219
                     /locus_tag="ACUQR0_002588"
     CDS             <1..219
                     /locus_tag="ACUQR0_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHV9109570.1"
                     /translation="SRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIE
                     YYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            321..473
                     /gene="hokA"
                     /locus_tag="ACUQR0_002589"
     CDS             321..473
                     /gene="hokA"
                     /locus_tag="ACUQR0_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HHV9109571.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(450..521)
                     /gene="ysaE"
                     /locus_tag="ACUQR0_002590"
     CDS             complement(450..521)
                     /gene="ysaE"
                     /locus_tag="ACUQR0_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HHV9109572.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(660..872)
                     /gene="cspE"
                     /locus_tag="ACUQR0_002591"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(660..872)
                     /gene="cspE"
                     /locus_tag="ACUQR0_002591"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HHV9109573.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1153..1443)
                     /locus_tag="ACUQR0_002592"
                     /pseudo
     CDS             complement(1153..1443)
                     /locus_tag="ACUQR0_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1877..2587
                     /locus_tag="ACUQR0_002593"
     CDS             1877..2587
                     /locus_tag="ACUQR0_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HHV9109574.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="ACUQR0_002594"
     CDS             complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="ACUQR0_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HHV9109575.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3715..4375)
                     /locus_tag="ACUQR0_002595"
                     /pseudo
     CDS             complement(3715..4375)
                     /locus_tag="ACUQR0_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4528..6861
                     /gene="bisC"
                     /locus_tag="ACUQR0_002596"
     CDS             4528..6861
                     /gene="bisC"
                     /locus_tag="ACUQR0_002596"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HHV9109576.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6830..7270)
                     /locus_tag="ACUQR0_002597"
     CDS             complement(6830..7270)
                     /locus_tag="ACUQR0_002597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HHV9109577.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7267..7830)
                     /gene="tag"
                     /locus_tag="ACUQR0_002598"
     CDS             complement(7267..7830)
                     /gene="tag"
                     /locus_tag="ACUQR0_002598"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HHV9109578.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7988..8686
                     /locus_tag="ACUQR0_002599"
     CDS             7988..8686
                     /locus_tag="ACUQR0_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HHV9109579.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8915..10123
                     /locus_tag="ACUQR0_002600"
     CDS             8915..10123
                     /locus_tag="ACUQR0_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HHV9109580.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10447..12138
                     /gene="eptB"
                     /locus_tag="ACUQR0_002601"
     CDS             10447..12138
                     /gene="eptB"
                     /locus_tag="ACUQR0_002601"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HHV9109581.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12230..12306
                     /locus_tag="ACUQR0_002602"
     tRNA            12230..12306
                     /locus_tag="ACUQR0_002602"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12264..12266,aa:Pro,seq:cgg)
     gene            13050..14597
                     /gene="dppA"
                     /locus_tag="ACUQR0_002603"
     CDS             13050..14597
                     /gene="dppA"
                     /locus_tag="ACUQR0_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HHV9109582.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWHDNKEFKPTREMNADDVVFSF
                     DRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDDNTVQFVLTRPEAPFLADLAM
                     DFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQYQKDSRIRYKAFDGYWGTKPQ
                     IDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARMKQDKSINLMEMPGLNVGYLS
                     YNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSAKNLIPPTMWGYNDDVQDYTY
                     DPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRMAEMIQADWAKVGVQAKIVTY
                     EWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSCAASEQGSNYSKWCYKPFEDL
                     IQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTVFEPVRKEVKGYVVDPLGKHH
                     FENVSIE"
     gene            14905..15924
                     /gene="dppB"
                     /locus_tag="ACUQR0_002604"
     CDS             14905..15924
                     /gene="dppB"
                     /locus_tag="ACUQR0_002604"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HHV9109583.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15934..16836
                     /gene="dppC"
                     /locus_tag="ACUQR0_002605"
     CDS             15934..16836
                     /gene="dppC"
                     /locus_tag="ACUQR0_002605"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HHV9109584.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16847..17830
                     /gene="dppD"
                     /locus_tag="ACUQR0_002606"
     CDS             16847..17830
                     /gene="dppD"
                     /locus_tag="ACUQR0_002606"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HHV9109585.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17827..18831
                     /gene="dppF"
                     /locus_tag="ACUQR0_002607"
     CDS             17827..18831
                     /gene="dppF"
                     /locus_tag="ACUQR0_002607"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HHV9109586.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18861..20132)
                     /locus_tag="ACUQR0_002608"
     CDS             complement(18861..20132)
                     /locus_tag="ACUQR0_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HHV9109587.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20608..>20714
                     /locus_tag="ACUQR0_002609"
     CDS             20608..>20714
                     /locus_tag="ACUQR0_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HHV9109588.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4984 a         5098 c         5444 g         5188 t
ORIGIN      
        1 tccagaaaag gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag
       61 tcggagtgtt tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg
      121 gaatatattg aatactataa cagcagaaga attagcctga aattaaaagg tctgactcca
      181 attgaatatc ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca
      241 gtacaaagtg aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa
      301 atcaacacag ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt
      361 gtttcacgct tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta
      421 aacaggagag ttatgagctg gcggcgtttt tagcctacaa attgaaagag taagagtctt
      481 cggcgggaaa ttattcccgc cttacttacg gcgttgcgca ttctcattgc acccatattt
      541 attcttcaca aaaataataa tagattttat tacgcgatcg attatttatt tcctgaaaaa
      601 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
      661 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
      721 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
      781 aacacatctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
      841 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
      901 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
      961 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
     1021 cgtcaaaccg ttgatgtgca ttaagccact cattggcggg tgatgcaaca attatttttc
     1081 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
     1141 gtggataaaa gtctatttca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
     1201 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
     1261 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttca
     1321 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
     1381 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
     1441 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
     1501 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
     1561 tcagcctgtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1621 tcttatcact cctgttttgt aaagatttcg caaatctaac cttctgcgaa ggcgtaaatc
     1681 tctggctaca aaaaaggcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     1741 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     1801 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     1861 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     1921 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     1981 agcgtttatc gacttcctgc aaaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     2041 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     2101 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     2161 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     2221 tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     2281 gcactctgcg ctaaaacaga gcgatgacct gaaaccggta tttgatcagg cattcaccaa
     2341 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     2401 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     2461 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     2521 gccattaccg gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     2581 ccaataaaga gtaaaagccc gagcgattat cgggctttac ttcacgcagt cgcagcttag
     2641 tccgcgacgt gaggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     2701 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     2761 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     2821 tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgctgc gatcagtgca
     2881 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     2941 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     3001 acgaaatctg actcttgtaa cagagtatcc aaatcgcagt agcgggcgtt gaagcgttct
     3061 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     3121 acacgttgtg ccagcgccat gccgatccgt cccatcccga caatgcccag tgttttatgg
     3181 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     3241 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     3301 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     3361 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     3421 attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc ttcagcttct
     3481 gcaaaaattg ctgcattttg aacgacggtt tgtgggctga ggtttgccac ctggtgaacg
     3541 gtgaaatgcg cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3601 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     3661 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     3721 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     3781 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     3841 ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcaggtc
     3901 gtgacccccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     3961 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg gagcgctgct
     4021 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     4081 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     4141 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     4201 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     4261 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     4321 taccgccaga gcgccactca ctacggcggc aagtaagata aacacgtttc ttcattgtta
     4381 atccttaata acctttttat tctttgtcac gggttccgtg gcgggagatt atgccgcgtg
     4441 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4501 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4561 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4621 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     4681 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     4741 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     4801 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     4861 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     4921 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg
     4981 gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt
     5041 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     5101 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg
     5161 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     5221 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     5281 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5341 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5401 aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5461 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5521 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5581 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     5641 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat
     5701 aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     5761 aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac
     5821 tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg
     5881 gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt tctgcctgcg
     5941 actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg
     6001 gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     6061 gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt
     6121 cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag
     6181 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     6241 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat
     6301 ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt
     6361 tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6421 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6481 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6541 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6601 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     6661 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     6721 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     6781 aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     6841 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     6901 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     6961 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     7021 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     7081 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat
     7141 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     7201 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     7261 cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat cattcaccag
     7321 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7381 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7441 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cgggaaacgg
     7501 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7561 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7621 tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     7681 gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca tttcgaacag
     7741 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     7801 gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     7861 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     7921 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     7981 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     8041 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     8101 tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     8161 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     8221 tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     8281 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8341 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8401 cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8461 gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8521 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8581 gttgctcaat caaaatattg ccgcctatgc tgcgctaact caggcagaaa ataccactaa
     8641 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     8701 ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa
     8761 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8821 tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt tgtcggcaga
     8881 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8941 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     9001 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     9061 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     9121 gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc tgggattagg
     9181 cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct
     9241 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9301 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9361 gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt
     9421 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9481 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9541 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9601 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9661 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9721 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9781 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9841 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9901 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9961 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
    10021 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
    10081 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
    10141 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
    10201 aaaatttggg ccagttaaca ggcccttttt actgcttttt gtaacgctaa agtctctttt
    10261 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct
    10321 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10381 tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt
    10441 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10501 tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac
    10561 gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg
    10621 accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg
    10681 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    10741 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    10801 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    10861 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    10921 cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    10981 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    11041 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    11101 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca
    11161 taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg
    11221 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    11281 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11341 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11401 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11461 agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg tgagcagatt
    11521 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11581 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    11641 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    11701 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    11761 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    11821 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    11881 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    11941 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    12001 atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat
    12061 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa
    12121 gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    12181 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    12241 gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac
    12301 cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta gctcatgagg
    12361 atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag acgcgccagc
    12421 gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac
    12481 caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc cattaacgga
    12541 tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg cgttacttat
    12601 ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
    12661 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
    12721 aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata
    12781 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
    12841 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
    12901 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
    12961 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
    13021 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
    13081 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
    13141 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
    13201 ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
    13261 ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc
    13321 gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
    13381 cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc ttgccggagc
    13441 tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg actcgaccgg
    13501 aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg
    13561 ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
    13621 gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
    13681 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
    13741 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
    13801 cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
    13861 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
    13921 aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
    13981 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
    14041 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
    14101 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
    14161 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
    14221 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
    14281 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
    14341 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
    14401 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
    14461 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    14521 cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    14581 acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa aactgcctga
    14641 tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt
    14701 aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt gtcaacaatc
    14761 tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    14821 tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    14881 gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    14941 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    15001 gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg
    15061 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    15121 gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg ggaagagttc
    15181 gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt tgctacggca
    15241 gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt cgatcacact
    15301 gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    15361 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    15421 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    15481 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc cattgtgctg
    15541 ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    15601 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    15661 gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct ccaggtggga
    15721 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctggggcgc
    15781 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    15841 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    15901 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    15961 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    16021 aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc
    16081 aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct cgccccgcca
    16141 gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt cggtcgtgat
    16201 gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg
    16261 gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt cggcggcctg
    16321 gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag cctgctgttg
    16381 gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct ggcgctgacc
    16441 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat
    16501 cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt
    16561 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    16621 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    16681 gagtggggca ctatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    16741 accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat gggtgacggt
    16801 ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg cgttattaaa
    16861 tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg
    16921 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    16981 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    17041 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    17101 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    17161 ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg gcaacaaaag
    17221 tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg atccggcttc
    17281 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    17341 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    17401 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    17461 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc ataaaatcat
    17521 tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct tccatgcgcc
    17581 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    17641 acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga acggctgcct
    17701 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    17761 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    17821 cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    17881 aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg
    17941 gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    18001 tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac cggcggcgag
    18061 ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg
    18121 cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg taagaaagtt
    18181 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    18241 gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    18301 ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    18361 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    18421 caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta tgtctttatc
    18481 tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat gtacctaggc
    18541 cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca tccgtacacc
    18601 caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga acgcatcaaa
    18661 ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt caacgcccgc
    18721 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggaggt
    18781 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    18841 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    18901 atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    18961 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    19021 tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata
    19081 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    19141 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    19201 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    19261 gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc agaaacggct
    19321 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    19381 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatctgct
    19441 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac cgcagaaaag
    19501 aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg cgggaaggcg
    19561 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    19621 actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc accagacgca
    19681 atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    19741 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    19801 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    19861 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    19921 gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat cgctgcggta
    19981 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    20041 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    20101 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    20161 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    20221 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    20281 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    20341 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    20401 aagcaatcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
    20461 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    20521 tacctgacaa cataacgggg gctaatcttg aacctagacc actcaagaat agccgcgaaa
    20581 cgttgtcatt acaacacagg cggctatatg acgctcgcag agctgggcat ggccttctgg
    20641 catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt gaactggctg
    20701 aacaagcgga agta
//
LOCUS       DBKELA010000071        20712 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.071, whole genome shotgun sequence.
ACCESSION   DBKELA010000071 DBKELA010000000
VERSION     DBKELA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.071"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACUQR0_002610"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACUQR0_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HHV9109589.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACUQR0_002611"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACUQR0_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HHV9109590.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACUQR0_002612"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACUQR0_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HHV9109591.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACUQR0_002613"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACUQR0_002613"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HHV9109592.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACUQR0_002614"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACUQR0_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HHV9109593.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYEALGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACUQR0_002615"
     CDS             complement(8217..9110)
                     /locus_tag="ACUQR0_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HHV9109594.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACUQR0_002616"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACUQR0_002616"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HHV9109595.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAEH
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACUQR0_002617"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACUQR0_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HHV9109596.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACUQR0_002618"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACUQR0_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HHV9109597.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACUQR0_002619"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACUQR0_002619"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HHV9109598.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACUQR0_002620"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACUQR0_002620"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HHV9109599.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACUQR0_002621"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACUQR0_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACUQR0_002622"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACUQR0_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HHV9109600.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACUQR0_002623"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACUQR0_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HHV9109601.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACUQR0_002624"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACUQR0_002624"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HHV9109602.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="ACUQR0_002625"
     CDS             complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="ACUQR0_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HHV9109603.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4982 a         5723 c         5033 g         4974 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa gcgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgt gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac aacgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttctag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 agcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttagccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tg
//
LOCUS       DBKELA010000072        20690 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.072, whole genome shotgun sequence.
ACCESSION   DBKELA010000072 DBKELA010000000
VERSION     DBKELA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.072"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            82..351
                     /locus_tag="ACUQR0_002626"
     CDS             82..351
                     /locus_tag="ACUQR0_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHV9109604.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="ACUQR0_002627"
     CDS             345..710
                     /locus_tag="ACUQR0_002627"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHV9109605.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="ACUQR0_002628"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="ACUQR0_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HHV9109606.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="ACUQR0_002629"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="ACUQR0_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HHV9109607.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="ACUQR0_002630"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="ACUQR0_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HHV9109608.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="ACUQR0_002631"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="ACUQR0_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HHV9109609.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="ACUQR0_002632"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="ACUQR0_002632"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HHV9109610.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="ACUQR0_002633"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="ACUQR0_002633"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HHV9109611.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="ACUQR0_002634"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="ACUQR0_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="ACUQR0_002635"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="ACUQR0_002635"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HHV9109612.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="ACUQR0_002636"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="ACUQR0_002636"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HHV9109613.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14858..16456
                     /gene="rtcR"
                     /locus_tag="ACUQR0_002637"
     CDS             14858..16456
                     /gene="rtcR"
                     /locus_tag="ACUQR0_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HHV9109614.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16438..17196)
                     /locus_tag="ACUQR0_002638"
     CDS             complement(16438..17196)
                     /locus_tag="ACUQR0_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HHV9109615.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="ACUQR0_002639"
     CDS             complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="ACUQR0_002639"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HHV9109616.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="ACUQR0_002640"
     CDS             complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="ACUQR0_002640"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate-cyanide
                     sulfurtransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HHV9109617.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18604..20109
                     /gene="glpD"
                     /locus_tag="ACUQR0_002641"
     CDS             18604..20109
                     /gene="glpD"
                     /locus_tag="ACUQR0_002641"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HHV9109618.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20315..20596)
                     /locus_tag="ACUQR0_002642"
     CDS             complement(20315..20596)
                     /locus_tag="ACUQR0_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109619.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIP"
BASE COUNT         4889 a         5689 c         5216 g         4896 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt tcagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcaccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaattttt taatttattg
    14761 tttctaaaaa gattatatct ttatgtccgt aaacggagat ttcccgcaaa gccgatttac
    14821 cgataatgaa atatcgcctt ttataaggat atctaagatg cgtaaaacag tggcttttgg
    14881 ctttgtcggt accgtgctgg attatgccgg acgcggcagt cagcgctggt caaaatggcg
    14941 tccgtcactc tgtatatacc agcaagaatc gttggtcatc gatcgactgg aattattgca
    15001 cgatgcccgc tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc
    15061 agaaacagaa gtggtgggcg ttgagattga actgcataac ccgtgggatt tcgaagaggt
    15121 ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa aagaagacta
    15181 tttaattcac atcaccaccg gcacccacgt cgcgcagatt tgctggtttc tgctggcgga
    15241 agcgcgttat ctacccgccc ggttgataca atcttcaact ccgcgcaaaa aagaacagcc
    15301 gcgcggccca ggtgaagtga cgattatcga tctcgattta agccgttata acgccatcgc
    15361 cagccgcttt gccgaggaac ggcagcaaac gctcgatttc ctcaaatccg gtattgccac
    15421 gcgtaacccc cacttcaacc gcatgattga gcagatcgaa aaagtggcga ttaaatcccg
    15481 cgcgccgatt ctgcttaacg gcccgaccgg cgcaggtaag tcatttctgg cgcgacgcat
    15541 cttcgagtta aaacaggcac ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc
    15601 caccctgcgc ggcgataccg cgatgtcgac gctgtttggt catgtgaaag gcgcgtttac
    15661 cggggcgcgg gaatctcgtg aaggattatt acgcagcgcc aacggcggaa tgttgtttct
    15721 tgatgagatt ggcgaactgg gcgcagacga acaggcaatg ctgctgaaag ctattgaaga
    15781 gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc agtgattttc agcttatcgc
    15841 cggaacggtg cgcgatttgc gccagctggt tgccgaaggc aaattccgcg aagatctgta
    15901 cgcgcggatc aatctctgga ccttcaccct gccgggtcta cgccagcgcc aggaagatat
    15961 tgaaccgaac ctggattatg aagtggagcg ccacgccaca ctcactggtg acagcgtgcg
    16021 ttttaacacc gaagcgcggc gtgcctggct ggcctttgca acatctcccc aggcaacatg
    16081 gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg atggccacct ttgccactag
    16141 cggacgcatc actctggacg tggtggaaga tgagataaac cgtctgcgct ataactggca
    16201 ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa acatcgatct
    16261 cttcgaccgt atgcaactgg aacacgttat cgctatctgc cgccaggcaa agtcgctttc
    16321 tgctgccgga cgacagcttt ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc
    16381 ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg tgcaggatca
    16441 gcacagctcc agttgaatat ggtggtccgt cagcacctgc atcacgctta ctggcggcgg
    16501 ggcgtcggtg tagacggcat caaccatgct gatgctcccc atattgacca tcgcattacg
    16561 gccaaatttt gaatggtcaa ccaccagcat aacgtggcgc gagttctcaa taatggcgcg
    16621 cttggtacga acttcgtggt aatcgaactc cagcagcgag ccgtcgctgt cgatgccgct
    16681 gatccccaga atgccgaaat caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc
    16741 aatgatcccg ccatcgcggc tacgtaattc gccaccggcg agaataatgc ggaaatcttc
    16801 ttttaccatc aacgtgttag caacgttgag attgttggtg acaatgcgca aattgctgtg
    16861 attgagcagc gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa acagcgtcga
    16921 gccattgggg atttgctccg ccactttgcg ggcgatgcgc tctttttctt cggtctgggt
    16981 ggctttacga tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg cgccgccatg
    17041 atggcgcagg atcaggtttt gctctgccag ctcgttgagg tcgcggcgaa tagtctgcgg
    17101 gctgacggag aagtgctcca ccagttcctc ggtactgaca taaccctgct gtttaaccag
    17161 ttcgataata ccgttgtgac gttgtgtttg tttcatttat aaatccctgg aattattttc
    17221 gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa acccacggct aatccggcga
    17281 tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa ccatccggcg acaatccaga
    17341 tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc
    17401 cacgtagcca gacgtagccc atcagcgcat acaccacgcc agaaagcccg ccaaaccacg
    17461 gcccgctgaa tttttgctgc atatagccgc ttaacagggc gctgataagc gtaatgacaa
    17521 ttagcttacc gctaccgagg cgtttttcca ccgcgccgcc gagataccac caccaaagca
    17581 ggttaaagag gatatgcatc agcgagaagt gcattaacgc gtgggtgaag taacgccaga
    17641 actcaaattt cagtgttgga tcgaatggcc aggccagcca taacatcact tcctgatcgc
    17701 cgagaatttg catggcaata aacaccacca cgcaggcgat catcatcacc caggtcaccg
    17761 gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg gcgataatgc aggccactgc
    17821 cggtatggcc tgccagccag ctggccgcca gataacgcgg atctgccggg ttttcgagaa
    17881 aacgcgccag ctccgcccgc acgcgctcgg cctgggactc atccgccagc cagacatcgc
    17941 tttggttatg ttgttgaatc gtgaggataa caccctgcgt cgccatgtaa tcaacaaacg
    18001 cctgcgccac gcgggggtta gcaaaagagg taatcatcaa catcgttgct gtcgcttatt
    18061 ccacacaaaa ggggacagta taaagcgtta cgcgccgtac gccacctctg cgggaaactg
    18121 tcgctgccag gcttcaaagc cgccgtcaat gctatagacc acatcgtagc cctgttgcag
    18181 cagatactgc gccgcccctt tgctgctatt gccgtgataa cacatcacca tcaccggagt
    18241 gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg
    18301 caccgcatgc cccatagcga aactctgtgg atcgcgaata tcgaccagca ccgcctcttt
    18361 ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat tcgaactgat ccatgcgtct
    18421 ctctttcttt acaaacaagt gggcaaattt accgcacagt ttacgtcgaa gcggcagata
    18481 aacgccataa tgttatacat atcactctaa aatgtttttt caatgttacc caaagcgcga
    18541 ttctttgcta atatgttcga taacgaacat ttatgagctt taacgaaagt gaatgagggc
    18601 agcatggaaa ccaaagatct gattgtgata ggtggcggca tcaatggtgc tggtatcgcg
    18661 gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc
    18721 gcgacctctt ccgccagttc aaaactcatt cacggtggcc tgcgctacct tgagcactat
    18781 gaattccgcc tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa aatggctccg
    18841 catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg
    18901 atgattcgca ttggtctgtt tatgtacgat catctgggta aacgcaccag cctgccggga
    18961 tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa gcgcggattc
    19021 gaatattctg actgttgggt agacgacgcc cgtctggtac tcgccaacgc ccagatggtg
    19081 gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac
    19141 ggcctgtgga ttgtggaagc ggaagatatc gataccggca aaaaatatag ctggcaagcg
    19201 cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat
    19261 ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc atattgtggt gccgcgcgtg
    19321 catacccaaa agcaggccta cattctgcaa aacgaagata aacgcattgt gttcgtgatc
    19381 ccgtggatgg acgagttttc catcatcggc accaccgatg tggaatacaa aggtgatccg
    19441 aaagcagtga agattgaaga gagtgaaatc aattacctgc tgaaagtgta taacgcgcac
    19501 tttaaaaagc agctaagccg tgacgatatc gtctggacct actccggtgt gcgtccgctg
    19561 tgtgatgatg agtccgactc gccgcaagcc attacccgtg attacaccct tgatatccat
    19621 gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg gtaagctgac cacctaccga
    19681 aaactggcag aacatgcgct ggaaaaactg acgccgtatt atcagggtat tggcccggcg
    19741 tggacgaaag agagcgtgct gccgggtggt gccattgaag gcgaccgcga cgattacgcc
    19801 gctcgcctgc gccgccgtta cccgttcctg actgaatcgc tggcacgtca ctacacgcgc
    19861 acttacggca gcaacagcga gctgctgctc ggcaatgcgg gaacggtaag cgatctcggg
    19921 gaagatttcg gtcatgagtt ctacgaagcg gaactgaaat acctggtgga tcacgaatgg
    19981 gtccgccgca ccgacgatgc cctgtggcgt cgcacaaaac aaggcatgtg gctgaatgcg
    20041 gatcaacaat ctcgtgtgag tcagtggctg gtggaatata cgcagcagaa gttgtcgctg
    20101 gcgtcgtaaa ttaacgtaag gtgatcaggt cagatttcaa tctggcctga gactgatgac
    20161 aaacacaaaa ctgcctgatg cgctacgctt atcaggccta cgtggtttat gcaatatatt
    20221 gaatttgcat ggtcttgtag gccagataag acgttcacgt cgcatccggc atgaacaaag
    20281 cgcactttgt caaaaatcta acctactttt taattcaggg aattaccgca aagcccacgc
    20341 catcataccc aacgtaacaa aactcaggca cgttccccac tcgccccgag aaaaatagca
    20401 ttaatgcgcc cagcgtcagc ataaaaatat tgagcagaag gtaaattgtt ggcgtaaaga
    20461 gtaaattgca aacctcagga atagcggcaa gcccagcccc cagcatggca ataaccgctg
    20521 caaaaaattg cgtcagtatt gggatgccga aaaaagtcat taccagcgag gtcaaaatcc
    20581 attttctatt ttgcattatt ctttccattc tttctgaatg gcgaaattat actccccgag
    20641 tccccttgcc ccttctggac acttttccga aatgatggcg aaaaaaacgg
//
LOCUS       DBKELA010000073        20614 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.073, whole genome shotgun sequence.
ACCESSION   DBKELA010000073 DBKELA010000000
VERSION     DBKELA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.073"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            98..397
                     /locus_tag="ACUQR0_002643"
     CDS             98..397
                     /locus_tag="ACUQR0_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHV9109620.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            407..931
                     /gene="ygaP"
                     /locus_tag="ACUQR0_002644"
     CDS             407..931
                     /gene="ygaP"
                     /locus_tag="ACUQR0_002644"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HHV9109621.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(978..1382)
                     /gene="stpA"
                     /locus_tag="ACUQR0_002645"
     CDS             complement(978..1382)
                     /gene="stpA"
                     /locus_tag="ACUQR0_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HHV9109622.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2050..2499
                     /gene="alaE"
                     /locus_tag="ACUQR0_002646"
     CDS             2050..2499
                     /gene="alaE"
                     /locus_tag="ACUQR0_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HHV9109623.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2536..2880)
                     /locus_tag="ACUQR0_002647"
     CDS             complement(2536..2880)
                     /locus_tag="ACUQR0_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HHV9109624.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3032..3361
                     /locus_tag="ACUQR0_002648"
                     /pseudo
     CDS             3032..3361
                     /locus_tag="ACUQR0_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            3609..3854
                     /gene="nrdH"
                     /locus_tag="ACUQR0_002649"
     CDS             3609..3854
                     /gene="nrdH"
                     /locus_tag="ACUQR0_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HHV9109625.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3851..4261
                     /gene="nrdI"
                     /locus_tag="ACUQR0_002650"
     CDS             3851..4261
                     /gene="nrdI"
                     /locus_tag="ACUQR0_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HHV9109626.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4234..6378
                     /gene="nrdE"
                     /locus_tag="ACUQR0_002651"
     CDS             4234..6378
                     /gene="nrdE"
                     /locus_tag="ACUQR0_002651"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHV9109627.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6388..7347
                     /gene="nrdF"
                     /locus_tag="ACUQR0_002652"
     CDS             6388..7347
                     /gene="nrdF"
                     /locus_tag="ACUQR0_002652"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHV9109628.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7702..8904
                     /gene="proV"
                     /locus_tag="ACUQR0_002653"
     CDS             7702..8904
                     /gene="proV"
                     /locus_tag="ACUQR0_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HHV9109629.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8897..9961
                     /gene="proW"
                     /locus_tag="ACUQR0_002654"
     CDS             8897..9961
                     /gene="proW"
                     /locus_tag="ACUQR0_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HHV9109630.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10019..11011
                     /gene="proX"
                     /locus_tag="ACUQR0_002655"
     CDS             10019..11011
                     /gene="proX"
                     /locus_tag="ACUQR0_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HHV9109631.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11203..12382
                     /locus_tag="ACUQR0_002656"
                     /pseudo
     CDS             11203..12382
                     /locus_tag="ACUQR0_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12506..13243
                     /gene="ygaZ"
                     /locus_tag="ACUQR0_002657"
     CDS             12506..13243
                     /gene="ygaZ"
                     /locus_tag="ACUQR0_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HHV9109632.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13233..13568
                     /gene="ygaH"
                     /locus_tag="ACUQR0_002658"
     CDS             13233..13568
                     /gene="ygaH"
                     /locus_tag="ACUQR0_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HHV9109633.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13659..14189
                     /gene="emrR"
                     /locus_tag="ACUQR0_002659"
     CDS             13659..14189
                     /gene="emrR"
                     /locus_tag="ACUQR0_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HHV9109634.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14316..15488
                     /gene="emrA"
                     /locus_tag="ACUQR0_002660"
                     /pseudo
     CDS             14316..15488
                     /gene="emrA"
                     /locus_tag="ACUQR0_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15505..17043
                     /gene="emrB"
                     /locus_tag="ACUQR0_002661"
     CDS             15505..17043
                     /gene="emrB"
                     /locus_tag="ACUQR0_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HHV9109635.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="ACUQR0_002662"
     CDS             complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="ACUQR0_002662"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HHV9109636.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="ACUQR0_002663"
     CDS             complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="ACUQR0_002663"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HHV9109637.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19401..19829)
                     /locus_tag="ACUQR0_002664"
     CDS             complement(19401..19829)
                     /locus_tag="ACUQR0_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HHV9109638.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="ACUQR0_002665"
     CDS             complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="ACUQR0_002665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HHV9109639.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4970 a         5276 c         5150 g         5218 t
ORIGIN      
        1 tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg catttttagt
       61 ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca
      121 ggccagtacc gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct
      181 gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac
      241 cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat
      301 caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc
      361 cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac
      421 catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg
      481 tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt
      541 gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca
      601 ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga
      661 aatattttta ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt
      721 aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat
      781 attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt
      841 tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt
      901 agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg
      961 acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc
     1021 tgagcaattg gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca
     1081 tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca
     1141 gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc
     1201 caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca
     1261 cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag
     1321 aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac
     1381 ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg
     1441 atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca
     1501 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
     1561 gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga
     1621 caaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt
     1681 gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg
     1741 acaaacaccg aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa
     1801 attaaaggca agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa
     1861 aatccacaat gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa
     1921 ataaaaattt ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca
     1981 gatggcaatg agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg
     2041 agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg
     2101 atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc
     2161 ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg
     2221 ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc
     2281 tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg
     2341 gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac
     2401 atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga
     2461 ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca
     2521 tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc
     2581 aacacgcgaa ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata
     2641 gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt
     2701 tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc
     2761 gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac
     2821 atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat
     2881 atttgatcct cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc
     2941 tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg
     3001 aaaattacat cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt
     3061 tgatgatggc gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc
     3121 tgtattgaag tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc
     3181 tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc
     3241 cgcgcgcgat gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt
     3301 gggtacagca gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta
     3361 gtgcaaaaat gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt
     3421 ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg
     3481 atcactattt gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg
     3541 tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa
     3601 tacaaatcat gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca
     3661 aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg
     3721 aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg
     3781 atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg
     3841 cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt
     3901 tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca
     3961 ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc
     4021 ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg
     4081 cgttattgct tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat
     4141 tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga
     4201 tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg
     4261 acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca
     4321 ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg
     4381 cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa
     4441 ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt
     4501 gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac
     4561 accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga
     4621 gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc
     4681 gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag
     4741 cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg
     4801 attggtcggg cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt
     4861 ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct
     4921 ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc
     4981 gcgcgtcagg gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt
     5041 ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc
     5101 gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt
     5161 tcgccctatg acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa
     5221 cactatgacg aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat
     5281 ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa
     5341 gacacagtaa accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca
     5401 gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca
     5461 ggccatgata tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc
     5521 gatttttccc gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt
     5581 catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg
     5641 gggcagatga atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa
     5701 gccctggatt tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg
     5761 atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc
     5821 agtggtgaat atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt
     5881 ggcgaactgt ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg
     5941 cgcgacgacg tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc
     6001 gggtctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta
     6061 gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac
     6121 gaaaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc
     6181 tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat
     6241 accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa
     6301 acgctctatt acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc
     6361 gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg
     6421 aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg
     6481 ctaccagaaa aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta
     6541 gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat
     6601 gttatcggcg cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta
     6661 tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg
     6721 ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg
     6781 cagcgaaaag ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa
     6841 atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat
     6901 ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat
     6961 gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct
     7021 ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac
     7081 aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa
     7141 gcgtttctct gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt
     7201 cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat
     7261 gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa
     7321 acagaagatg aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag
     7381 cagggaaata attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca
     7441 ctgatactct gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct
     7501 atgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc
     7561 ggcgatttgc tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct
     7621 cagtatgtta gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa
     7681 ataaaggaat cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata
     7741 tttggtgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa
     7801 attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa
     7861 ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt
     7921 ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc
     7981 aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag
     8041 tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg
     8101 gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg
     8161 ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg
     8221 ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg
     8281 gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa
     8341 catcagcgca ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac
     8401 cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc
     8461 aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg
     8521 ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc
     8581 ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt
     8641 atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta
     8701 acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa
     8761 acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc
     8821 gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat
     8881 cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga
     8941 cagtgccgcg caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg
     9001 tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga
     9061 tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt
     9121 tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa
     9181 cggtttccag caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct
     9241 catcacctgg cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc
     9301 catcggcgca atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc
     9361 cctgctgttc tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg
     9421 agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta
     9481 tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat
     9541 catctttgcg ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc
     9601 ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt
     9661 tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc
     9721 cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact
     9781 tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat
     9841 cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg
     9901 caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta
     9961 actctgcact tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat
    10021 gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc
    10081 tgccgatctg ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga
    10141 aaccttccag acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa
    10201 acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt
    10261 caccgccgtg aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa
    10321 gaaattttat cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa
    10381 gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc
    10441 caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta accctggctg
    10501 gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac
    10561 tcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg
    10621 caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg
    10681 gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga
    10741 taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc
    10801 caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt
    10861 gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg
    10921 cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg
    10981 ggtgaatgag gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg
    11041 cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta
    11101 caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta
    11161 ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat
    11221 gagcttagcc cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt
    11281 aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt
    11341 tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt
    11401 gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca
    11461 gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg
    11521 gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg
    11581 gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg
    11641 atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc
    11701 ttttgggttg cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca
    11761 caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg
    11821 ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac
    11881 ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc
    11941 gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg
    12001 gcgggcggtt ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt
    12061 actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat
    12121 tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg
    12181 aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg
    12241 cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt
    12301 ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca
    12361 tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag
    12421 ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct atttataata
    12481 ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc
    12541 ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt
    12601 ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt
    12661 tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc
    12721 cgggagtagt ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt
    12781 gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct
    12841 atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa
    12901 taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg
    12961 ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc
    13021 cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc
    13081 tttccagcgc aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt
    13141 aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc
    13201 gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt
    13261 tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta
    13321 atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct
    13381 cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt
    13441 tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca
    13501 ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga
    13561 ttatataacc cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc
    13621 gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca
    13681 ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc
    13741 ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc
    13801 tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg
    13861 aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg
    13921 cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata
    13981 acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg
    14041 ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa
    14101 aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag
    14161 acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat
    14221 aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc
    14281 tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa
    14341 ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct
    14401 tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt
    14461 tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt
    14521 ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc
    14581 tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg
    14641 cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata
    14701 ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc
    14761 tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca
    14821 gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc
    14881 tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg
    14941 cctagctggc gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc
    15001 gtgccgtgca accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag
    15061 ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg
    15121 gtcagccggt caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag
    15181 tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga
    15241 ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac
    15301 agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta
    15361 accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg
    15421 cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg
    15481 ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg
    15541 gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt
    15601 gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg
    15661 tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg
    15721 gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg
    15781 tcttgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag
    15841 gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg
    15901 ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt
    15961 tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc
    16021 atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt
    16081 gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt
    16141 atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag
    16201 gaaattatca ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag
    16261 ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc
    16321 ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg
    16381 cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg
    16441 gtagggatta ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat
    16501 atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc
    16561 tatacctttg aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg
    16621 tttgcggtgg tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg
    16681 gaacgactgg cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc
    16741 ggcacgtcga taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg
    16801 actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg
    16861 cttgggatga cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg
    16921 ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg
    16981 gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac
    17041 taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt
    17101 tactgactag atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct
    17161 gttgatgcgt acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga
    17221 gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg
    17281 cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg
    17341 cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat
    17401 ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt
    17461 atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac
    17521 ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc
    17581 catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat
    17641 ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg
    17701 catttgttat catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt
    17761 tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt
    17821 tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc
    17881 agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct
    17941 gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg
    18001 ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta
    18061 atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc
    18121 agtaacggga actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct
    18181 tcaaggatca cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc
    18241 ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca
    18301 tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg
    18361 ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga
    18421 atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta
    18481 ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta
    18541 aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta
    18601 ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg
    18661 cactcggttt tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc
    18721 gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata
    18781 acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac
    18841 ttcgcttgcc agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc
    18901 atcagcgcgc cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg
    18961 ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata
    19021 cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc
    19081 ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct
    19141 tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga
    19201 cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc
    19261 tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc
    19321 gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga
    19381 ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca
    19441 actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag
    19501 atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat
    19561 aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat
    19621 gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta
    19681 cccattgttg ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc
    19741 accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac
    19801 aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg
    19861 ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt
    19921 gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga
    19981 cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg
    20041 cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac
    20101 ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg
    20161 cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa
    20221 ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt
    20281 actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat
    20341 ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct
    20401 cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg
    20461 aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa
    20521 ggaaattttt tggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    20581 gtagccgagt actctatcca gctgagctac ggat
//
LOCUS       DBKELA010000074        20605 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.074, whole genome shotgun sequence.
ACCESSION   DBKELA010000074 DBKELA010000000
VERSION     DBKELA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.074"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACUQR0_002666"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACUQR0_002666"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HHV9109640.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACUQR0_002667"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACUQR0_002667"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HHV9109641.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACUQR0_002668"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACUQR0_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HHV9109642.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACUQR0_002669"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACUQR0_002669"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HHV9109643.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="ACUQR0_002670"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="ACUQR0_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HHV9109644.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="ACUQR0_002671"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="ACUQR0_002671"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HHV9109645.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="ACUQR0_002672"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="ACUQR0_002672"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HHV9109646.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="ACUQR0_002673"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="ACUQR0_002673"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HHV9109647.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="ACUQR0_002674"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="ACUQR0_002674"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HHV9109648.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="ACUQR0_002675"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="ACUQR0_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HHV9109649.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="ACUQR0_002676"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="ACUQR0_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HHV9109650.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="ACUQR0_002677"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="ACUQR0_002677"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HHV9109651.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="ACUQR0_002678"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="ACUQR0_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HHV9109652.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="ACUQR0_002679"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="ACUQR0_002679"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HHV9109653.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="ACUQR0_002680"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="ACUQR0_002680"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HHV9109654.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="ACUQR0_002681"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="ACUQR0_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HHV9109655.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="ACUQR0_002682"
     CDS             complement(18174..18569)
                     /locus_tag="ACUQR0_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HHV9109656.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="ACUQR0_002683"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="ACUQR0_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9109657.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="ACUQR0_002684"
     CDS             19485..20078
                     /locus_tag="ACUQR0_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHV9109658.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..>20605
                     /gene="tabA"
                     /locus_tag="ACUQR0_002685"
     CDS             20223..>20605
                     /gene="tabA"
                     /locus_tag="ACUQR0_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HHV9109659.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEV"
BASE COUNT         4900 a         5186 c         5464 g         5055 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgca
//
LOCUS       DBKELA010000075        20566 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.075, whole genome shotgun sequence.
ACCESSION   DBKELA010000075 DBKELA010000000
VERSION     DBKELA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.075"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..75)
                     /gene="ytiD"
                     /locus_tag="ACUQR0_002686"
     CDS             complement(<1..75)
                     /gene="ytiD"
                     /locus_tag="ACUQR0_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HHV9109660.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLR"
     gene            complement(72..155)
                     /locus_tag="ACUQR0_002687"
     CDS             complement(72..155)
                     /locus_tag="ACUQR0_002687"
                     /inference="COORDINATES: protein motif:HMM:NF048182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109661.1"
                     /translation="MPVNGIFDVFDMLSIYIIYKLIVSNNT"
     gene            409..1239
                     /locus_tag="ACUQR0_002688"
     CDS             409..1239
                     /locus_tag="ACUQR0_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HHV9109662.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="ACUQR0_002689"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="ACUQR0_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HHV9109663.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="ACUQR0_002690"
     CDS             complement(2368..3828)
                     /locus_tag="ACUQR0_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HHV9109664.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="ACUQR0_002691"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="ACUQR0_002691"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HHV9109665.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="ACUQR0_002692"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="ACUQR0_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HHV9109666.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="ACUQR0_002693"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="ACUQR0_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HHV9109667.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8392)
                     /locus_tag="ACUQR0_002694"
     CDS             complement(7949..8392)
                     /locus_tag="ACUQR0_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9109668.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="ACUQR0_002695"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="ACUQR0_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HHV9109669.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="ACUQR0_002696"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="ACUQR0_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HHV9109670.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="ACUQR0_002697"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="ACUQR0_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="ACUQR0_002698"
     CDS             complement(12470..13009)
                     /locus_tag="ACUQR0_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9109671.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="ACUQR0_002699"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="ACUQR0_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HHV9109672.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="ACUQR0_002700"
     CDS             complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="ACUQR0_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HHV9109673.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="ACUQR0_002701"
                     /pseudo
     CDS             complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="ACUQR0_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17233..17949
                     /gene="nanC"
                     /locus_tag="ACUQR0_002702"
     CDS             17233..17949
                     /gene="nanC"
                     /locus_tag="ACUQR0_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HHV9109674.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17969..19075
                     /gene="nanM"
                     /locus_tag="ACUQR0_002703"
     CDS             17969..19075
                     /gene="nanM"
                     /locus_tag="ACUQR0_002703"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HHV9109675.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19362..19706
                     /locus_tag="ACUQR0_002704"
                     /pseudo
     CDS             19362..19706
                     /locus_tag="ACUQR0_002704"
                     /inference="COORDINATES: protein motif:HMM:NF015585.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            complement(20039..20137)
                     /locus_tag="ACUQR0_002705"
     CDS             complement(20039..20137)
                     /locus_tag="ACUQR0_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109676.1"
                     /translation="MNKLPAHLSRQNCKIASTNLSEIIPRRAAVLK"
     gene            20230..20435
                     /locus_tag="ACUQR0_002706"
                     /pseudo
     CDS             20230..20435
                     /locus_tag="ACUQR0_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
BASE COUNT         5477 a         4908 c         4672 g         5509 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    14041 acagtttggc cccaattgtc ttgtgtttat ttgtttttta aatttattat caataagtta
    14101 aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
    14161 gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgattcccg
    14221 agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca
    14281 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga
    14341 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg
    14401 tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca
    14461 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
    14521 attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc
    14581 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
    14641 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
    14701 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
    14761 caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa
    14821 ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
    14881 atttgattca atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa
    14941 gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt
    15001 atttattttg aagaacataa accattagat tttgtatggg tatggttaac ttaatgttta
    15061 ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt
    15121 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
    15181 aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
    15241 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctagata
    15301 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc
    15361 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
    15421 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
    15481 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
    15541 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
    15601 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
    15661 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
    15721 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
    15781 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
    15841 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
    15901 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
    15961 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
    16021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
    16081 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
    16141 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
    16201 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
    16261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
    16321 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
    16381 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
    16441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
    16501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
    16561 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
    16621 aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
    16681 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
    16741 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
    16801 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
    16861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
    16921 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
    16981 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
    17041 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
    17101 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
    17161 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
    17221 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
    17281 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
    17341 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
    17401 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
    17461 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
    17521 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
    17581 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
    17641 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
    17701 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
    17761 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
    17821 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
    17881 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
    17941 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
    18001 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
    18061 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
    18121 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
    18181 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
    18241 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
    18301 ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
    18361 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
    18421 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
    18481 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
    18541 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
    18601 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
    18661 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
    18721 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
    18781 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
    18841 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
    18901 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
    18961 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
    19021 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
    19081 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
    19141 tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg
    19201 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
    19261 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgacatgt cactttaacg
    19321 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
    19381 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
    19441 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
    19501 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
    19561 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
    19621 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
    19681 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
    19741 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
    19801 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
    19861 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
    19921 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
    19981 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
    20041 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
    20101 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
    20161 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
    20221 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
    20281 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
    20341 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
    20401 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
    20461 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
    20521 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgca
//
LOCUS       DBKELA010000076        20521 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.076, whole genome shotgun sequence.
ACCESSION   DBKELA010000076 DBKELA010000000
VERSION     DBKELA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.076"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACUQR0_002707"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACUQR0_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HHV9109677.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACUQR0_002708"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACUQR0_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HHV9109678.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACUQR0_002709"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACUQR0_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HHV9109679.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACUQR0_002710"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACUQR0_002710"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HHV9109680.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACUQR0_002711"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACUQR0_002711"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HHV9109681.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACUQR0_002712"
     CDS             4449..4814
                     /locus_tag="ACUQR0_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HHV9109682.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="ACUQR0_002713"
     CDS             4964..5482
                     /locus_tag="ACUQR0_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HHV9109683.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="ACUQR0_002714"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="ACUQR0_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="ACUQR0_002715"
     CDS             6713..7195
                     /locus_tag="ACUQR0_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HHV9109684.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACUQR0_002716"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACUQR0_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HHV9109685.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACUQR0_002717"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACUQR0_002717"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HHV9109686.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACUQR0_002718"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACUQR0_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HHV9109687.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACUQR0_002719"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACUQR0_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HHV9109688.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACUQR0_002720"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACUQR0_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HHV9109689.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="ACUQR0_002721"
     CDS             10939..11730
                     /locus_tag="ACUQR0_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HHV9109690.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="ACUQR0_002722"
     CDS             11751..13037
                     /locus_tag="ACUQR0_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HHV9109691.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACUQR0_002723"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACUQR0_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HHV9109692.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="ACUQR0_002724"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="ACUQR0_002724"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HHV9109693.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="ACUQR0_002725"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="ACUQR0_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HHV9109694.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="ACUQR0_002726"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="ACUQR0_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HHV9109695.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="ACUQR0_002727"
     CDS             complement(16985..17275)
                     /locus_tag="ACUQR0_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HHV9109696.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACUQR0_002728"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACUQR0_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HHV9109697.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="ACUQR0_002729"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="ACUQR0_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HHV9109698.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="ACUQR0_002730"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="ACUQR0_002730"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="ACUQR0_002731"
     CDS             19137..20378
                     /locus_tag="ACUQR0_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HHV9109699.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5303 a         5358 c         4814 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgagccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DBKELA010000077        20478 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.077, whole genome shotgun sequence.
ACCESSION   DBKELA010000077 DBKELA010000000
VERSION     DBKELA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.077"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="ACUQR0_002732"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="ACUQR0_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HHV9109700.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACUQR0_002733"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACUQR0_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HHV9109701.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACUQR0_002734"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACUQR0_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HHV9109702.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACUQR0_002735"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACUQR0_002735"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HHV9109703.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="ACUQR0_002736"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="ACUQR0_002736"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HHV9109704.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5834)
                     /gene="prfH"
                     /locus_tag="ACUQR0_002737"
                     /pseudo
     CDS             complement(5235..5834)
                     /gene="prfH"
                     /locus_tag="ACUQR0_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="ACUQR0_002738"
     CDS             complement(5911..6954)
                     /locus_tag="ACUQR0_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HHV9109705.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="ACUQR0_002739"
     CDS             complement(7200..7652)
                     /locus_tag="ACUQR0_002739"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9109706.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACUQR0_002740"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACUQR0_002740"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HHV9109707.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACUQR0_002741"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACUQR0_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HHV9109708.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACUQR0_002742"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACUQR0_002742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HHV9109709.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACUQR0_002743"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACUQR0_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HHV9109710.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10285..12003
                     /locus_tag="ACUQR0_002744"
                     /pseudo
     CDS             10285..12003
                     /locus_tag="ACUQR0_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACUQR0_002745"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACUQR0_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HHV9109711.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13420)
                     /locus_tag="ACUQR0_002746"
                     /pseudo
     CDS             complement(12893..13420)
                     /locus_tag="ACUQR0_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13798..14538
                     /gene="dpaA"
                     /locus_tag="ACUQR0_002747"
     CDS             13798..14538
                     /gene="dpaA"
                     /locus_tag="ACUQR0_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HHV9109712.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14509..15276)
                     /locus_tag="ACUQR0_002748"
     CDS             complement(14509..15276)
                     /locus_tag="ACUQR0_002748"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HHV9109713.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="ACUQR0_002749"
     CDS             complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="ACUQR0_002749"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HHV9109714.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16300..18744
                     /gene="fadE"
                     /locus_tag="ACUQR0_002750"
                     /pseudo
     CDS             16300..18744
                     /gene="fadE"
                     /locus_tag="ACUQR0_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="ACUQR0_002751"
     CDS             complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="ACUQR0_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HHV9109715.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19414..20184
                     /gene="yafV"
                     /locus_tag="ACUQR0_002752"
     CDS             19414..20184
                     /gene="yafV"
                     /locus_tag="ACUQR0_002752"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HHV9109716.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20294..20478
                     /locus_tag="ACUQR0_002753"
                     /pseudo
     CDS             20294..20478
                     /locus_tag="ACUQR0_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         5007 a         5265 c         5264 g         4942 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt
    13561 attaattgct tatttgcata ttactattga gatttcataa tattaggaaa taaatgatac
    13621 atgcgctttt cggactattg aaaactgccc ctgtgctaaa tttgtaaacc cagcgccgac
    13681 aggcttgcgt aaaactcttt tggtgataat gtgagccttc caaatccaga ctattccgat
    13741 ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg
    13801 cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg
    13861 ctgggcagca gtagctcgac cacgccagtg agcaaagagt ataagcagca gttgatggga
    13921 tcccctgtct acatccagat cttcaaggaa gaacgtacgc tcgatcttta cgtcaaaatg
    13981 ggcgagcaat atcaactgct cgacagttat aaaatctgta aatattccgg cggcttaggg
    14041 ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat
    14101 cagttaaaac cagacagccg ttactacaaa gcgattaata ttggattccc caatgcttat
    14161 gaccgtgcgc atggttatga agggaaatac ctgatgatcc acggcgattg tgtatccatc
    14221 ggctgctacg caatgaccaa tcagggtatt gatgagatct tccagttcgt tactggtgcg
    14281 ctggtgtttg gtcagccgag cgtgcaggtg agtatttacc cattccgcat gaccgacgcc
    14341 aatatgaagc gccataaata ttccaacttt aaggacttct gggagcagct gaagccgggc
    14401 tacgactact ttgagcaaac ccgtaagcca ccgaccgttt ctgtggccaa tggccgttac
    14461 gtggtcagca agccgttaag ccacgaagtg gtgcagccac aactggcatc aaactacacg
    14521 ctccccgagg caaaataagt gccattcgcc tggcataatt ttttgccagg tttcattgcc
    14581 cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa
    14641 gtcgatttcc acatcctgat ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat
    14701 ccagtgtaaa ttagtcgagc aatacgccat tacatagcgc ccgtccgaaa gcagcatgtt
    14761 gaaaacgccc ttcgcccgca gttcatccgc cagtgaggcg atatatttaa acactgccgc
    14821 catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta tgcagcagcc agcaaaaggc
    14881 tttttcgctg tcggtttcac cgaccgggcg gaagttgccg gtttccagtg atttgtagcc
    14941 cgtcagttgt ccgttatggg cataagtcca gttacgcccc cataactcgc gggtaaatgg
    15001 gtgagtattt tccagcgcca cctcgccccg attagcctgg cgaatatgag ccaccaccga
    15061 acaggatttt atcgggtagt cctggacaag tttggcgatg ggggaattaa agctgggttg
    15121 tggatcttta aatgtgcgac agcctttacc ttcgtaaaag gtaatgcccc agccatcttt
    15181 atgtggcccg gttccaccac cacgctgtac aagtccggtg aaactaaagc agatatcggt
    15241 agggacgttg gcgctcatcc cgagcaattc gcacatgggt aacctccaca acagcgggag
    15301 cgcatggacc ccggcgaaga ttgatttaag aattttcagg tcggatgcgg cgtaaacgaa
    15361 ttattcggcc tacggttcgg gtacagattt gtaggcatga taagacgcgt cagcatcgca
    15421 tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc cttatccggc ctacgccaga
    15481 cttacttaac catctctttt tcaattaact ggatcaggat atggatcact ttaatgtgaa
    15541 tctcctgaat gcggtcggca taaccaaagt gcggaacgcg aatttcgata tccgccgtgc
    15601 cagccatttt accgccgtct ttaccggtca gggtgatcac tttcatcccc ttctcacgcg
    15661 ccgctgcgat cgctttgatc acgtttgcag agttaccgga ggtggagatc cccagcagta
    15721 catcgccttc gcgacctacc gcttcaacgt agcgggagaa aatatcattg aaaccgaaat
    15781 cattaccgac gcaggaaata tgactaacgt cagaaatagc aatcgccggg tagcccggac
    15841 ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg catagcgtcg caatgggaac
    15901 cgccgttgcc gcaggaaagc actttgccac cggctttaaa gctgtctgct aacaggaccg
    15961 ccgcgcgctg aatggcgtga atattggcgt catcttttaa aaagttagcc agcgtttccg
    16021 ccgcttcgtt cagttcgtta cgaataagat cctggtacat gaggatatcc ttcagcataa
    16081 atgtaataga caaaatgcag tgtaccggat accgccaaaa gcgagaagta cgggcaggtg
    16141 ctatgaccag gactttttga cctgaagtgc ggataaaaac agcaacaatg tgagctttgt
    16201 tgtaattata ttgtaaacac attgctaaat gtttttacat ccactacaac catatcatca
    16261 caagtggtca gacctcctac aagtaagggg cttttcgtta tgatgatttt gagtattctc
    16321 gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg tgagcttatt tatcagcagt
    16381 ctgattttgc tcgcctagac agccgccctc ggcgttgctg gtctgtggtc ggcgtgggta
    16441 ctggtgcctc tgaccattat cctcgtgcca tttaactttg cgcctatgcg taagtcgatg
    16501 atttccgcgc cggtatttcg cggtttccgt aaggtgatgc cgccgatgtc gcgcactgag
    16561 aaagaagcga ttgatgcggg caccacctgg tgggagggcg acttgttcca gggcaagccg
    16621 gactggaaaa agctgcataa ctatccgcag ccgcgcctga ccgctgaaga gcaagcgttt
    16681 ctcgacggcc cggtagaaga agcctgccgg atggcgaatg atttccagat tactcatgag
    16741 ctggcggatc tgccgccgga gttgtgggcg taccttaaag agcatcgctt cttcgcgatg
    16801 attatcaaaa aagagtacgg cggactggag ttctcagctt atgcccagtc tcgtgtgctg
    16861 caaaaactct ctggcgtgag cgggatcctg gcgattaccg tcggcgtgcc aaactcatta
    16921 ggcccgggcg aactgttgca acattacggc actgacgagc agaaagatca ctatctgccg
    16981 cgtctggcgc gtggtcagga gatcccctgc tttgcactga ccagcccgga agcgggttcc
    17041 gatgcgggcg cgattccgga caccgggatt gtctgcatgg gcgaatggca gggccagcag
    17101 gtgctgggga tgcgtctgac ctggaacaaa cgctacatta cgctggcacc gattgcgacc
    17161 gtgcttgggc tggcgtttaa actctccgac ccggaaaaat tactcggcgg tgcagaagat
    17221 ttaggcatta cctgtgcgct gatcccaacc accacgccgg gcgtggaaat tggtcgtcgc
    17281 cacttcccgc tgaacgtacc gttccagaac ggaccgacgc gcggtaaaga tgtcttcgtg
    17341 ccgatcgatt acatcatcgg cgggccgaaa atggccgggc aaggctggcg gatgctggtg
    17401 gagtgcctct cggtaggccg cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa
    17461 tcggtagcgc tggcaacggg cgcgtattct cacattcgcc gtcagttcaa aatctctatc
    17521 ggtaagatgg aagggattga agagccgctg gcgcgtattg ccgggaacgc ttatgtaatg
    17581 gatgctgcgg catcgctgat tacctacggc attatgctcg gcgaaaaacc tgccgtgctg
    17641 tcggctatcg ttaagtatca ctgtacccac cgcgggcagc agtcgattat tgatgcgatg
    17701 gatattaccg gcggtaaagg cattatgctc gggcaaagca acttcctggc gcgtgcttac
    17761 cagggcgcac cgattgccat caccgttgaa ggggctaaca ttctgacccg cagcatgatg
    17821 atcttcggac aaggagcgat tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg
    17881 aagaacaatg acgtcaacgc gttcgataaa ctgttgttca aacatatcgg tcacgtcggt
    17941 agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca
    18001 accggcgatg ccactaaacg ctactatcag cacctgaacc gcctgagcgc caacctcgcc
    18061 ctgctttctg atgtctcgat ggcactgctg ggcggcagcc tgaaacgtcg tgagcgtatc
    18121 tcggcacgtc tgggggatat tttaagccag ctttacctcg cctctgccgt tctgaagcgt
    18181 tatgacgacg aaggccgtaa tgaagccgat ctgccgctgg tgcactgggg cgtacaggat
    18241 gcgctgtatc aggcagaaca ggcaatggac aatttactgc aaaacttccc gaaccgcgtg
    18301 gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac gtcattatct ggcaccgtct
    18361 gacaagctgg atcataaagt ggcgaagatt ttacaagtgc cgaacgccac tcgttcacgt
    18421 attggtcgcg gtcagtacct gacgccaagc gagcataacc cggttggttt actggaagag
    18481 gcgttggtgg atgtgattgc cgccgacccg attcatcagc ggatctgtaa agagctgggt
    18541 aaaaatctgc catttacccg tctggatgaa ctggcgcaca acgcgctgac gaaggggctg
    18601 attgataaag atgaagccgc tattctggtg aaagcggaag aaagccgtct gcgcagtatt
    18661 aacgttgatg actttgatcc ggaagagctg gcgacgaagc cggtaaagtt gccggagaaa
    18721 gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttctttatc
    18781 cgctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc
    18841 gaacagcacc gttttaccat caatcgaaag cgcatcgttc acattcagcc aggtgagttt
    18901 ctcttgcgac gttttctcat caatagccga gaacagcccc gtcatctgat tagatttctc
    18961 ggaccacatc acagcgatac gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac
    19021 ctgatacgtc tcatctccca acgttacggt ttgtgcggga gtataagtac cgcctttcat
    19081 cacccatgca ggtagcttat gcccttgcac catctgatta aacgcagctt tggtggtttc
    19141 gccctttgca aggctgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac
    19201 cagtgcggcg actgtcgtta ttgccttaaa catcattcct cccgagctta tcctgcccat
    19261 gttattaacc tcctaaagcc tggcgataac acgataattt gcaaatcaga tatttccaat
    19321 atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttaaagt
    19381 tataaaaccc ttcgcataaa ggagttgtac accgtgccta gtttgaagat tacgcttttg
    19441 cagcaaccgt tggtgtgggt ggatggtcct gccaacctgc gccattttga tcgtcaactg
    19501 gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc
    19561 atggaagcgg cagcttcgtc gcttgcacaa gatgacgtag tgaactggat gacagctaag
    19621 gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat tacaaacgga gtctggttcg
    19681 gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat
    19741 ctgttccgca tggcagatga gcatctgcat tataaagcgg gcaatgcgcg agtgattgtg
    19801 gaatggcgcg gctggcgtat tttgccgctg gtgtgctacg acttacgttt tccagtgtgg
    19861 tcgcgcaatc tcaacgatta tgacctcgcc ctgtacgtcg ccaactggcc agctccgcgc
    19921 tctctgcact ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg
    19981 ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt atcgcggtga cagccgggtg
    20041 attaatccgc aaggcgagat tatcgctacc gccgacgcgc ataaggcaac gcgtattgat
    20101 gcggagctgt cgatggtggc attgcgggaa tatagagaaa agtttccggc atggcgggat
    20161 gcggatgagt ttaggttgtg gtgagtgggg gttgtgttta gggccggggg ctgggctaga
    20221 ttacgaaagc ccctaactta aacacaatat tacgccagaa gtcaccattt ccaaaacaca
    20281 gcgtcgctgt accgtgggtt atctgcccaa ccgcagcgac acgccggtga tcaaaatcag
    20341 cgccaaatgg ctgagaaaag cgggatttga gatcggcacg ggcgttaccg tgaagatctc
    20401 ggagggatgt ttgatcctgc ttgggataac aacgaagtac aggaactgcg cagggaactt
    20461 tatcaggtta aacagcca
//
LOCUS       DBKELA010000078        20410 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.078, whole genome shotgun sequence.
ACCESSION   DBKELA010000078 DBKELA010000000
VERSION     DBKELA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.078"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..248
                     /locus_tag="ACUQR0_002754"
                     /pseudo
     CDS             1..248
                     /locus_tag="ACUQR0_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            921..2429
                     /gene="ltrA"
                     /locus_tag="ACUQR0_002755"
     CDS             921..2429
                     /gene="ltrA"
                     /locus_tag="ACUQR0_002755"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHV9109717.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2510..2997
                     /locus_tag="ACUQR0_002756"
                     /pseudo
     CDS             2510..2997
                     /locus_tag="ACUQR0_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3080..3337
                     /locus_tag="ACUQR0_002757"
     CDS             3080..3337
                     /locus_tag="ACUQR0_002757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109718.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(3895..4662)
                     /gene="fecE"
                     /locus_tag="ACUQR0_002758"
     CDS             complement(3895..4662)
                     /gene="fecE"
                     /locus_tag="ACUQR0_002758"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HHV9109719.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4663..5619)
                     /gene="fecD"
                     /locus_tag="ACUQR0_002759"
     CDS             complement(4663..5619)
                     /gene="fecD"
                     /locus_tag="ACUQR0_002759"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HHV9109720.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5616..6614)
                     /gene="fecC"
                     /locus_tag="ACUQR0_002760"
     CDS             complement(5616..6614)
                     /gene="fecC"
                     /locus_tag="ACUQR0_002760"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HHV9109721.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6611..7513)
                     /gene="fecB"
                     /locus_tag="ACUQR0_002761"
     CDS             complement(6611..7513)
                     /gene="fecB"
                     /locus_tag="ACUQR0_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HHV9109722.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7558..9882)
                     /gene="fecA"
                     /locus_tag="ACUQR0_002762"
     CDS             complement(7558..9882)
                     /gene="fecA"
                     /locus_tag="ACUQR0_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HHV9109723.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9969..10922)
                     /gene="fecR"
                     /locus_tag="ACUQR0_002763"
     CDS             complement(9969..10922)
                     /gene="fecR"
                     /locus_tag="ACUQR0_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HHV9109724.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10919..11440)
                     /gene="fecI"
                     /locus_tag="ACUQR0_002764"
     CDS             complement(10919..11440)
                     /gene="fecI"
                     /locus_tag="ACUQR0_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HHV9109725.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11577..11645
                     /gene="ykiE"
                     /locus_tag="ACUQR0_002765"
                     /pseudo
     CDS             11577..11645
                     /gene="ykiE"
                     /locus_tag="ACUQR0_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
     gene            11716..11920
                     /locus_tag="ACUQR0_002766"
                     /pseudo
     CDS             11716..11920
                     /locus_tag="ACUQR0_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11920..12018
                     /locus_tag="ACUQR0_002767"
                     /pseudo
     CDS             11920..12018
                     /locus_tag="ACUQR0_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12334..12615
                     /locus_tag="ACUQR0_002768"
     CDS             12334..12615
                     /locus_tag="ACUQR0_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HHV9109726.1"
                     /translation="MTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAV
                     NALFCERDIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(12740..12870)
                     /locus_tag="ACUQR0_002769"
                     /pseudo
     CDS             complement(12740..12870)
                     /locus_tag="ACUQR0_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856299.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12935..13066)
                     /locus_tag="ACUQR0_002770"
                     /pseudo
     CDS             complement(12935..13066)
                     /locus_tag="ACUQR0_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13348..14706
                     /locus_tag="ACUQR0_002771"
     CDS             13348..14706
                     /locus_tag="ACUQR0_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HHV9109727.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(14828..14905)
                     /locus_tag="ACUQR0_002772"
                     /pseudo
     CDS             complement(14828..14905)
                     /locus_tag="ACUQR0_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            complement(14945..16330)
                     /gene="tnpC"
                     /locus_tag="ACUQR0_002773"
     CDS             complement(14945..16330)
                     /gene="tnpC"
                     /locus_tag="ACUQR0_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HHV9109728.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(16380..16727)
                     /gene="tnpB"
                     /locus_tag="ACUQR0_002774"
     CDS             complement(16380..16727)
                     /gene="tnpB"
                     /locus_tag="ACUQR0_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HHV9109729.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(16724..17104)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_002775"
     CDS             complement(16724..17104)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_002775"
                     /inference="COORDINATES: protein motif:HMM:NF047595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HHV9109730.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(17459..17893)
                     /locus_tag="ACUQR0_002776"
     CDS             complement(17459..17893)
                     /locus_tag="ACUQR0_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HHV9109731.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(17881..18282)
                     /locus_tag="ACUQR0_002777"
     CDS             complement(17881..18282)
                     /locus_tag="ACUQR0_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HHV9109732.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(18542..19111)
                     /locus_tag="ACUQR0_002778"
     CDS             complement(18542..19111)
                     /locus_tag="ACUQR0_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HHV9109733.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            19310..19510
                     /locus_tag="ACUQR0_002779"
     CDS             19310..19510
                     /locus_tag="ACUQR0_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109734.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVCILCSW"
     gene            complement(19856..20032)
                     /locus_tag="ACUQR0_002780"
     CDS             complement(19856..20032)
                     /locus_tag="ACUQR0_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HHV9109735.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
BASE COUNT         4777 a         5421 c         5574 g         4638 t
ORIGIN      
        1 aactgcatgg catcagccac ggctctgccg gagcaagaag catcgccaca atggcaaccc
       61 agagaggcta ccagatgggg cgctggcttg ctggcagact catgaaagag ctggggctgg
      121 tcagttgcca gcagccgact caccggtata agcgtggcgg tcatgagcac gttgctatcc
      181 cgaatcatct tgagcgacag ttcgccgtaa cggaaccaaa tcaggtgtgg tgcggtgatg
      241 tgacctatag tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct
      301 gtctggaata ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac
      361 ggtggagaac ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga
      421 caacggggat tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag
      481 gctgaagaac atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac
      541 gtgatctgac aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag
      601 gtgttttgac atgtatgcga aacaccgggg cagcagcgtc tatcacgctt gcgttgctga
      661 cttctgccaa cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc
      721 ctgagtccag gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc
      781 gatgacgcgc tgactggcgg agcttccgta gtagtccgcg atggggaaag cccattacat
      841 ggcgaaggga agcagtttga atgtgtttgc gacgtgaatt aactggccta acgaggtgaa
      901 gacctttgat aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt
      961 ccctacggat tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg
     1021 cgcggatcac gctttcatca aagggggcgc atacccccgg cgttgatggc gtgaacaaaa
     1081 caatgctaca ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag
     1141 gccactacca gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc
     1201 gaccactggg tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga
     1261 tggagccgat atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca
     1321 gtgtccacca cgcgatccgc acggtgaaat tacagctcac agactgcggt gaaacccggg
     1381 gacgctgggt gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc
     1441 tgatgaaggc tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa
     1501 ccatcaaggc gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac
     1561 agggcggtgt tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc
     1621 tgcatgagcg ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta
     1681 tccaacgggg ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt
     1741 actgccgcta tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag
     1801 ccatcaggga ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg
     1861 ataagactaa aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc
     1921 gcaaacgcag tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca
     1981 gaaacttcgc cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg
     2041 atatggctga acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg
     2101 attttaaggc caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact
     2161 ggctggcccg caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac
     2221 cggaaccggg tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca
     2281 gcggcgaaat actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac
     2341 ccgaaggtaa tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag
     2401 aagtggcaat ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg
     2461 tccggttcgg ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact
     2521 ctggacgggt aagcgctggg cgtacctcgc cgttgttctc gacctgttcg caagaaaacc
     2581 agtgggctgg gccatctcgt tctcgccgga cagcaggctt accatgaaag cactggaaat
     2641 ggcatgggaa acccgtggta agcccgtcgg ggtgatgttc caagcgatca aggcagtcat
     2701 tatacgagca ggcagttccg gcagttactg tggcgatacc agatcagaca gagtatgagc
     2761 cggcgtggaa actgctggga taacagccca atggagcgct tcttcaggag tctgaagaac
     2821 taatgggtgc cagcgacggg ctatgtaagc ttcagcgatg cagctcacgc aataacggac
     2881 tatatcgttg gatattacag cgcactaaga ccgcacgaat ataatggtgg gttaccacca
     2941 aacgaatcag aaaaccgata ctggaaaaac tctaacgcgg aggccagttt tagttgacca
     3001 caacagacta cctgaaggga gccgcggtcg cctagcagtt gcagtagcag gagatcatcc
     3061 agccgcagta caggtcacga tgactctggt taatgatacc ggctttgacc ccgtattttc
     3121 cggctctatc gctgaatcat ggcgtcagca gccgtgcaca ccatcctatt gttgtgactg
     3181 ggaggctgcc accatgcttc gcgctttccc tctggcgaaa aagggagaag gacgggcccg
     3241 tctgccttca ctttatgcca gcttcggtaa gctgggtgag acaccgactc atgaagatat
     3301 cattgataac aatcgatcca tcaactggcc tgtataacgt ggctgccggt gattaagaaa
     3361 gctgcaccta cctaagtagt agcaaacgca cactttttag aaaaatcgat ggtcagaaac
     3421 tggattagca attccgttcc agggttgctt ttgatttacg ttggcgtctg atcattgatt
     3481 tatcctcaaa agcccaacct cattggtaat gaaccagctc cgtgaatgtc cgctctggca
     3541 cagagcgaaa ttttttgatc tccccccctg aaatctaaac ttagtcatgt cacgtttttg
     3601 ggtttctaaa attttaactt cgcgtttttc gttgccgtaa gggttataca gaaatgtccg
     3661 ttaagcagag ttcaaaattg attgccgtga tcacgactgg tttgaaagcc gcgcccaagc
     3721 ctgtacagct ctggtttgcg ttgattatga acctgtcagc ctaaagcaag cggatggacg
     3781 atgagtattg gtaatctttc agagtccgga aaagttcagc cccagtctga acaggcttgc
     3841 tggcgccagt ccagtttcat tcagtcgtgg tttggttctt acggcctgtg caatctacct
     3901 cattaggcac atcggcctgc cagataccgg ctcggggtgt atttccgctt ccacgctgaa
     3961 tactgttctc agcaatcctg gggtcatcac ctcttctggt gtgccttgcg ccataacatg
     4021 tccgtttgcc attaccacca gttgatcgca gtaccggcta gcctgattaa ggtcgtgcag
     4081 cacagcgacc accgttttcc cctgagtccg gagttcgccc atcaaccgca tcaggtccac
     4141 ctggtgattg atatcaagat aggtggttgg ctcatcaagt aatacaacgg gcgtattctg
     4201 ggccaggacc atcgccagaa atgcgcgctg gcgctgaccg ccggaaagct cggttaaccg
     4261 acgaacggca agatgattga tccgggtctg gttcatggcg acattaactc gtgcattgtc
     4321 ttcagcggag agacgccccc agagtgacag ccagggatta cgaccatacg aaaccagctc
     4381 ctggactgtg atcccctctg gcgttaaatg gtgctgaggc agcagcgaaa gcctgcgggc
     4441 caactggcgt gatgagagca tatttatggg attatcgccg agaaatacgg tgccagactg
     4501 cggcattaaa agccgcgaaa aacagtttaa cagcgtcgat ttcccgcaac cgttagggcc
     4561 gatcagggcg gtgatcttcc ccgttggcag tgagagtgaa acgtcgttaa gtaccttgtc
     4621 tgtcccgtaa ctgaccgtca gattttcagt tcgtaaagtc atttatcgca ttctcacaag
     4681 caaccagaca aaccacggcg caccgataat ggcggtcagc acgccaaccg ggagctccag
     4741 tgggggatga ataattctcg ccagcagatc ggcaaccacc aacagcaacg cacctgtcag
     4801 ggctgaaaca ggcagcagtc tgcggtgacg tccaccggtg atgctacgca tcatatgcgg
     4861 caccacgaga ccaataaagc taatcgggcc gcaggcggcc acgccggtag atgtcatggc
     4921 gacagctagt aacaaagccc agaatcgggt atggggcacc gacacaccga gcgtggtggc
     4981 gcgcgcatcg ccgagtgcaa ggaggtcgag atcgcggcaa aaactcaggc tcagcggcag
     5041 aaataaaatc atcagcggga tggcaatctt cacaaagctc cagtcacggc cccataagct
     5101 gccggtcagc cacagcaggg cgttgttcac atcctgcggg cgcgagagca tcagataatc
     5161 cgtcaggctg gcccagcatg cagaaagcgc cacgccggtg agcgccagct tcatcggctg
     5221 gtgggtcttt gccagcatct tcagtaatat caaccccgcc atgccgcccg caaaggccag
     5281 cagcggcagc accatcacgg gcagtgacgg cataagaagt agagccccca cagaggccag
     5341 gctggcggca tggttaacgc cgagaatatc cggtgatgcc agagggttgc gcacaatccc
     5401 ctgtatcagc acgcccgcca cggcgagggc tgcaccgaca aacagtgcca gcagcaagcg
     5461 cggcagtcgg tactccatca atacataata atgctcgcgt ccggcctgcc agtcggtcag
     5521 cagcgcgcgc cacggcacgg ggatcactcc catatggagt gataacagcg cacagcccgc
     5581 cagggcaagg gtgatgaaaa taaccagcgc aattttcatc ctcgcctcct cacaagccag
     5641 acaaagcaag ggctgccaat cagcgccagc actgcgcctg cgggcagatc tccggggaag
     5701 gccagcgcgc gtgcgagtac atctgccagc agcatcagcg tggcccccag cagcatgctc
     5761 actggcagta cgttgcgctg atcgaagcct gcccagaagc gcgccagatg tggcaccagc
     5821 agaccgataa acgccaccgg acctgccacg ctgacgcacg caccaaccag aagcagcact
     5881 aacatattga tgaccaaacg tagcctcgtc aggttcactc ccagcgtatg ggcggtgctg
     5941 tcgctgaggt tgagcaggtt cagttgattc gccagcagta acacgacagg gactgcagtg
     6001 accaccaccg gcaagagctg ccagacatcc tgccagcggg cgtgggacac tccgcctgcc
     6061 agccagtaaa agatgccgta agcatgatct tcggccagca gcagggtgat gcgggtaagg
     6121 cccatacaaa aggccgacag cgcgataccc gcgaggatca gtttgtttct gtcatgggta
     6181 tgacgaaatc cgcctcctgc ggtcatgacc agcagccagc tcacgccgcc cccgcatgcc
     6241 gcgatgaacg acagagaata gcctgcaatc ggcgtcggac tcagcgcgct ggtaagcgcc
     6301 atagccagcg ccgcgccgct gttaatgccg agcagtgaag gagaggccat tgggttgtgg
     6361 gtcagggttt gcagcagcgt gcccgcgagc gccaggcttg cgccgatcag aacggcgacc
     6421 aggcttcgtg gcaaacgaag gttttgcacc agcgcttctg gtagcgttgg cgtgtgtcca
     6481 ggcagcaggg cgcgggttgc atctgctccg gaaacaggaa tggccgagta gcaaaacaga
     6541 ctcagccaga aaataataat aagtgctgca acgggaagcc cccacagcag catcgggtgt
     6601 tttatcgcgg tcatttcaca acggtaagcg gctgatggtg gaagattttt accgtgtcag
     6661 cggcaatacg ctctgcagca aaaataccgc gcatccgcgc ccaggtgtta ctgtcgaccg
     6721 aagcaacctg ctgcttctgc gcggcggtta acatctgcca gagcggatct tgttgccagc
     6781 gtttaacaat gctctcttcg cgatagtggg caaccagcag ccaggcagga ttgaccgcca
     6841 gcagttgctc caggccgatg gacggcatgg acgaacccgc catcgcagcg ggaacgttca
     6901 gccccagaga ggccagcacg ctgccggtcc aggtctcctg agtatgcagg ttgaattgct
     6961 gttcgcgtga tgtgccaaag gccacgcgtg tccctttggg aagctggctg gcccactgcg
     7021 ccatcctctc tttatgttgt tccagacgtg cctgcatctc tcgcttttta cccaccattt
     7081 cgccgatgat agccgcagat tgcaaatttt cagcgtaggt ttcgttgcgg gacttaagca
     7141 gcagtaccgg cgcgatttgc tgcaaggcga tgtaaacccc cgcatggcga ctgctgtcgg
     7201 caatgatcag gtctggtttc agagcggcaa tggcttccag gctcggctgc gcgcgcgttc
     7261 cgacggactg ccacggtttc aggtgcgcac gcacttcggg caggatgcgt tttgcatcgt
     7321 tatcgtcggc aataccgatc gggctgacgt ccacggcggc cagcgcatcg gcgaacgaga
     7381 gttccagcac cacaatccgt tgtggcgttt tttcgagtgt aaacgtgccg tgttcgtcct
     7441 gaaccatggc ggcaaaggcg tggctgatca ccagcagcag gcctgcaaaa agaaaacgga
     7501 taaatgccaa cataatcaca ttccagctaa aagcccggca agccgggcgt taacacatca
     7561 gaacttcaac gacccctgca tatacagcgt gcgcggctgg cctgcataga tgcctttgtt
     7621 gttgtcgtca taagagcgga tgaagtagtc ctggtcgaag atgtttttca caccgaatgc
     7681 caggttcaga tctgccatct gcgggccaaa gtcatacgcc acgcgtgcac cccagagcat
     7741 gaagccggga atgcggccgg tactgccgtc ggcgctctct ttcaccgtat tggcgttatc
     7801 cgcaaactgg ctggactgga aatcgctgtt cagattgaac gtccaatttc ctggcttgta
     7861 gtccacgccc agcgtgcctt tatgtttcgg ggagaatggt accagattgc cgtaggtgtc
     7921 gcctttctcg cggatttccg cgttcacata cgcatagctg gcgtagatgg aaacgttatc
     7981 aagcgttggc gttagcgtac ccagatcgta acgtgcctgc gtttccagcc cggtatggcg
     8041 cgttttgcca cgtgcagtga cggtgtcgtt ggtctggttg gagtcgtact gattgttaaa
     8101 gttaatcagg aacagcccca tttccgccgt cagcgcgccg tcgtcgtagc gggtaccgag
     8161 ttcccaggtt cgcgcttttt ccggttccac attgccgctt tgcacagcct tgccaatctg
     8221 gctgtactgt acggtgccga acgagccttc agtgtttgca taaagattcc agctgtcagt
     8281 caggtgatag agcacgttca acgccggaag cggtgcgtta tagctcactt cttcgtgcgt
     8341 gcctgtgatg gcgttgttct ggtatgactc gatatgttcg aaacgcatac ccggcgtgat
     8401 ggtccagttg ccgatgtcga ttttgtcatc cagataccag gcgtgcgcct cggtgccgga
     8461 acgcgtatcg cggtcgtaag ggcttgagcc ggacggcaac tgcccgctgc tggtggcggt
     8521 gtagtaacgc atttcatgcg ttgattcatt cacatagcga tagcccacgc ccacttcgtg
     8581 cgcggaaggg ccgatcataa agatctggct gtagcgtggc tcaataccgc gcacccagta
     8641 gttacgcggc gagagggtga tgcgtttgcc ttgctccagg tagccgctgc gcagggtttg
     8701 ggtgtagaac ccctgaatgt tgaatttatg ctggctgtct ggctggaact ggtagcccag
     8761 gctcgccagc ttgcgacgac cccagaagcg gtcatacggg cgggtggatt gccagcgatc
     8821 ggcgtcgtaa tccgcgcgag acaggccacc gggcatgtcg gcttcaccgt cgtaatattg
     8881 cagcaggctg ttgaaggtgt gcacctcatc cggcgcatat ttgcttttca gcatcaggtc
     8941 gtcgatgcgg gtggcgctgt gctcgcgcca gtcactgccg cgcgtgccgg agtagagcag
     9001 cgcggtgcca aaaccgttgt ccgctgtgcc gcccaccatc aggttgtgcg tctctttcgg
     9061 gttgttttgt gaagaggttg ggctgagctg accttccacg cccgcctcga taccaaagtc
     9121 ctgcggaatg gcacgggtaa caaagttcac cacgccgccc acgctctgcg gtccgtaacg
     9181 caccgcacca ccaccgcgta ccacgtcaat ggcatccatg ttgccgagcg aaatgggagc
     9241 cagtgaaagc tgcggctgac cgtaaggggc gaaggggacg gggatgccgt ccatcaggac
     9301 ggtcgagcgg ctggcgaggc gcgggttcag gccccggatg ccaaagttca tcgccaggtc
     9361 gtggctgccg gtgccgttgt tttccggcgc gctgacgcca gggatgcggt taagtacctc
     9421 acgcatggtg gttgcgccgg ttttggcgaa atcctcacgg cggatcacgt cacgcgcgcc
     9481 agcatgttca aatacgtcgt tttcacgcgc atcacccagc cagtcgccga ccacggtcag
     9541 ggcatcttct tttggtgcgg gcgcgggctc cagcgtccag ctgttatttc ccagcggttt
     9601 tacctgcagt ccgctgccgt ccagcagttg ttgcaggccg ctctcgacgt cgtaatcgcc
     9661 gtgcaggccg ttgctctgct tgccgcgcgt caggctggcg tcaaccgaga gggtaaatcc
     9721 gctgtgtgcg gcatactgat tgagcgcttt atcgagcgat cccggtgcga tattaacctg
     9781 tgcagcaaaa gcggaaaacg agagaccggc cagcggcagc aggctcaggc gaatggcgtt
     9841 aaccaaaggt gttgttttac gaaaaacgcg taacggcgtc ataccttccc catcatcatt
     9901 tttgttgtgt tcagctatga gtcgaacgag aagggtaaaa aggacaatcg aaataagaat
     9961 tattttcctt acagtggtga aatgtttatc cagtaccgcg taatagactg aattttaacg
    10021 ggaagcgttt gcgcgataac gttcaggatc gcatcggtat ttttcagcgg gaacgtcccg
    10081 ctcaggcgca gcccggcaac ggcgggatcg cagcgcagca cgccgttgcg gtaacgggtt
    10141 agcgtggcta tcacctcacc cagcggttta tcgctgaagc tcaggatgtc cttcgtccag
    10201 cttgtactct cgtcatccag cggtttcact gcgccaaact cagaggcgct gaactgcagg
    10261 ctttcaccag cgttcacgat gcgtttttgc gcgggggcac tggcgagaag cacttccaca
    10321 gcgtgctgct gaacgtcaag ctgcgtgaaa ttatcctgct ggcggacggt aaattctgtc
    10381 cctaaagcag tgagctggcc ctgacgggtc aggacacgga aggggcgttg cagggcatct
    10441 ttcgcggtag taatggcgat ttcaccgtac cagagccgga cggtgcgctg atgcgcatca
    10501 aaacgcacat ccgccgcgct ttgggtattc agcgtgagca gggagccatc ttccagttgc
    10561 tgacggctga ctgtgccttt ggcggtgcgg taatctgccc gcagaccttc gccggtttcc
    10621 gactgccaga gctgccagcc tccgccagcg ccgagcaaca gcagtaatcc tttcatcacg
    10681 tgacggcggg tgaggcgggt atcgtgcaac gcccggctgg caacgtcgcc aggcacaccg
    10741 ccaagctggt tgcgcaggtt ttcaacctgc tgccaggccc actggttatc ctgatcctgt
    10801 tcataccact gttgccagcg cgcttcctgt tgtgggctga cgcgctcgcc gcttagcacg
    10861 gcataccagt gggaagctga acgcagcgcc tgacggcggg aatcggttaa caaaggattc
    10921 ataacccata ctccagacgg aacagcaggc agtgctcgac ggctttcgcc acgtattttt
    10981 tcacggagct gatggaaaca ccgagtttgt gcgcaatctc gctgtatgtc agaccatcca
    11041 gttgcgaaag cagaaacgct tcacgtgttt tgccgtttag cccgtccagc atgctgtcga
    11101 ggagttgtag ggtctcgagt tggctttcgc gttcctcagg tgaaggcgct cccccctccg
    11161 gcataagcgc cagcatctcc agatacgctt tttccagcgc gtttcggcga aacaggtcca
    11221 ccatcacgcg tttggcgata gtgcagagga aggagcgagg atcgcggatc gtcgagagcg
    11281 tttcgctgac cattacccgc aaaaaagtgt cctgggcaat gtcatctgca tcaaaagcag
    11341 actggagttt gcgcgtcagc cagcttttca accagccgtg atgtgtgcca taaagcgact
    11401 cgaacgttaa ggaagctgtg gtagtggcgc ggtcagacat gcggagtgca tcaaaagtta
    11461 attatcacgt agtcatatta atatgagaat ggttatcatt acaattggaa ataaaattgt
    11521 ttccaataga catttttaac atgttgtttt tctaagtgtt ataaggtagg tataaaatgg
    11581 gatggagcct ctgcttctga catgtgtcgg tcagaatgac tcatgatgtg gtctgctatt
    11641 attgacatcc tcactgccct aaaggatggg gatttcggta atgatcttac ccagcaatag
    11701 tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga caaaacagtt
    11761 tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag atctgaagct
    11821 tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg aatcaccaca
    11881 gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta tatcgagtgc
    11941 gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga acaatttgaa
    12001 aatcataatc tcacttaaaa cggcatccat tttacaaaag atggccgtct gtgtatttgt
    12061 aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata tcctaaaaat
    12121 tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga gttgactgaa
    12181 tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa ccattagtct
    12241 gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa aatatgcttg
    12301 ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg gagaaagatg
    12361 cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt atatgaagct
    12421 aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc tttattctgt
    12481 gagcgtgata taataaacaa caagaacata ataaagaagc tgatgttctt tttgtcagat
    12541 gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat tgacattaca
    12601 tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt atctgaaaac
    12661 gaaatatata gtccctctcg ttttgtaata tcgattggct ttgttgaatc ggttacgtac
    12721 cccggagccg gtcatatttt caggtaatac acacgctttc agacatttcg gctttcgtca
    12781 tttttgtatc atgggcatag cttctgctac ctgaccatca taatcacgca gcttcagtga
    12841 agcagtgacc acctgtttaa cccggtgtat cgttatgatc ttatccagca attgaaccgc
    12901 cccgggtttc ctggagagtg ttttatctgt gaactcaggc tgccagatca tcgtttccga
    12961 tggaagcata ataagctttt tctgcttctg ccggtgggat atggccctgc ctttccagca
    13021 atcgtcgatt gttataccag tccacccacg tgagtgtggt cagctcatca tactgacacg
    13081 tgcattaccc ctaccgcgat aatgcaaccg tacaatatag ggcgtttcct aaacgcagcc
    13141 ctcatcactc caatccaaca agacacagca tttttgatca cgaagaaaga caaataattt
    13201 ccgtaaatgt ttccgttgtt acaaataaca acagtgtttt acatttcttt ggcctcaggc
    13261 ccgcgcatgt tgccattcga tgtcattttc ctgactcgtt tcttctttat ggtgtagtgt
    13321 gatcttcact ttatagatgc taacaatatg aaaagaaaaa ttattcccgt gctaatcggt
    13381 tgtacgctct ctttctctgc cctggcggca caacctacgg ctgagcgcta tgtcgtcagt
    13441 tttccagaag gcacgcacgt gaactacgcc ggcgcatttg ccagtgcatt cccgaacggc
    13501 cttccggttg ggataggttc tggcctgctg ttcaccggta aacagggcga tgccctgaca
    13561 ttcgcaacca ttaccgatcg cggtccgaac gcggattcac cgaaagaggg gaaaaacgaa
    13621 actaaaattt tcgttacgcc agatttcgcg ccgctgctga tgacgattcg tgtacaaaac
    13681 ggcaaagcgg aggccatcga tccccgtcct ctgcatgacg ataaaggcgc gatcaacggt
    13741 ctgccgctgg caagcgacgt gattggctcc accaatgaag tggccttcag cgacacgtta
    13801 catagactga agggggataa tcgcgggctg gatacggaag ggatcactcc ggatggtaaa
    13861 ggaggttact ggctgtgcga tgagtatggc ccgttcctga ttaacatcga cagtaaaggg
    13921 aaaatcctgg caatccacgg tccgcaggcg gcggaagggg aaaaagcgat tgcgggcggt
    13981 ttgccaaata tcctcaaatg gcgtcaggcg aaccgcggct ttgaaggcct cacccgtatg
    14041 ccggacgggc gtattattgt cgccgtgcaa agtacgctgg atatcgacgc taagagcaag
    14101 aaaaaggcgc tgtttacgcg tctggtgagc ttcgatccgg caagcggaaa aaccgcgatg
    14161 tacggttatc ctatcgacag cgcggcctac agcaaaaaca gcgacgctaa aatcggagac
    14221 atcgtggcgc tcgataacca gcacatcctg ctgatcgaac agggaagaga taaaaacaac
    14281 agaatgcgta acctgattta cgaggtcgat ctcaataagg cgagcgacct gtcaggcttc
    14341 gacaagccgg gtgagtatcc ggagtttgat gatgagaaaa ccctgtcaca gcgcggcatt
    14401 acccttgcgc agaaaacgca ggttgttgat ctgcgctcct tgggctggca gcaggagaag
    14461 gctgaagggt tggcgctgat cgacagcaaa acgctggcgg tggcgaacga taacgacttt
    14521 ggcgtgaagg tggcgatgca acatccggtc gagggcaaga cattcaagga ttatcgggtg
    14581 aacgcggaag gaaagctgac gcttgacgac aagcaggttg aaacgaccct cagagtgaag
    14641 ccactggaga aaccggagtc tgacagtgaa ctgtggattg tgacgctgcc ggaagcgttg
    14701 aaataagttt ataaggtacg gacagcagta tcgcttagtt tttcaaattc atgtatgtga
    14761 acaatattgg ggttccggcg tagaccccca atgaaagcgt caccacgtga aaacatgcct
    14821 gcacggttta cacgtcatcc catggtggca gccggttaac tttcctgcgc caccagtgga
    14881 aaaaggccat aagcagtccg aacaaaagta agcgtacagc gagggccgta ttgacgggga
    14941 tgtgttattc agctggcagt gctatgcgcc acggaagcag ttcgctgacc cggttgaccg
    15001 gccagtctgc tatgacgcca agcacatggc gaaggtagct ttctggatcc acgtcattca
    15061 gtttgcacgt cccgatcagg ctgtacagta gcgctccccg ctcaccacca tggccagagc
    15121 cgaagaacag ggtcttcatc ttttcacgca accagctttc cagggatttc aacagcggtt
    15181 tcgtttttcg ctgacgttca gcaagccgct gctctgccgg cattcccctt atatccgcct
    15241 ctatggcgta caactgaccg atctgctcca gggcttcttc cgtcagtgct gacgggatgc
    15301 ggacgtgcac atcgtggatc tttcggcggg catgagccca gcaggcagct tccgttatcc
    15361 caccattgcg atacagctcg ttgaacccgg cgtacgcatc cgcttgcagc acaccgctga
    15421 agcaagcaag atgagtctgc ggatggatgc tttttctgtc cgggctgtaa gcgaaccaca
    15481 ctgcaggtgc caacgctgac ccggcattgc ggtcatcacg aacatacgcc cacaaccgcc
    15541 cggtcttcgt cttcttatta cccggcagca gtacctggac cggggtatca tcggcatgga
    15601 gtttgccgtc agtcatgaca tagccatgaa gcgcctcttc cagcggagac agcagccggc
    15661 agcatgcatc cacccagccc gacagcagtg aacggctcag ctccacacct tgccggccgt
    15721 atatttctga ctggcaatac agcggggtgt gctctgcata cttcgaggtc agcacgcggg
    15781 ccagcagccc cggtccggcg ataccccgct cgatgggccg cgaaggtgca ggtgcctgca
    15841 cgatggtatc gcactgagta caggcatgtt tttcccgtac cgtccggata acccggaagg
    15901 cgctacgcat caactccagc tgttcggcgg tatcctcgcc cagatagctc agtgaaccgc
    15961 cgcagttcgg gcagcacggc gccgcaggca acagtcgctt ttcgtcacgg ggtagtgatt
    16021 cagggaacgg cttacgggtg cgggtctgac gcaacggacg ctgtactgcc gggtcataca
    16081 ccctaccagt cagcgtatcg ctctctttct gaagccggtt cagatcggct tccatttgtg
    16141 cgatacggcg ggagactttt tcggaacgac tgccgaagtt catccggcgg agtttatcca
    16201 gctgcgcctg cagatggtct atttcgcgct cccggttgct cagcttttcc tgcagggcgt
    16261 ggatcagcgc ttcctgttcg gccaggcgct gtttcagcag gaagatgtcg tcagaagaga
    16321 tgtcgttcat aagcccgtat tttaccgggc ttattctgtg acaaccagga taaagagatt
    16381 tacagcatgg tcagggaggt cagcagccgc ttgggctgtc gccagtcgat accttccagc
    16441 agcatcgcca gctgcgcctg cgtaaggaac actttgccat cacgggctga cggccaggcg
    16501 aagcgcccac gctccagccg tttggtcagg aggcacagtc cgtcgccggt ggaccacagc
    16561 agtttaacct gactgccgct gcggccccgg aaaatgaaaa tatggccgga catgggatcg
    16621 tctttcagcg ccgtttgtac tttcgcagcc aggccgttga agctatttct catatcggtg
    16681 ataccggcaa ccagccaaat tttggtcccg gaaggtaacg ggatcatcgc ttcagttcct
    16741 gtatcagcag agtcaggagc ttttcgctga cattgccatt gaagcggagc gtcccgtgcc
    16801 ggaacgttac ctcacagctg atactgaggg tttccgggtc ctctgcgagc gattctggct
    16861 gttcggcagc tgcatcgaga gtcacaggaa gtagctgggg gctctctgaa gaaggtaata
    16921 gcagctttcc ctcgcgccat tgttgtcgcc atttgaacaa cagattggcg ttaatgccat
    16981 tttcaagagc aagttttgag atggatatcc cgggttcaca ggaggcagca acgagcagct
    17041 gtttaaattc gggaggataa ttagggcagc cttttcgcct gccgggagtc acatttttct
    17101 gcatatctga cactttggtt cccactactt atttggtgga caccactttg tctaattcgt
    17161 cagattctga ccagacggtt caggctgtac gcttaccact attttatccc tgttgaattt
    17221 ggttaagccg gtgcagacgc gccgggaaac gcacacctga accggcacaa taaaatacaa
    17281 tacaaccccc tgttactgga gatacgaatg gaatgacgag gcagcctgtc agtcacaggg
    17341 ggctgttggc ataaaatcaa cggaaaggcc accaacaccg gaattaagct ccacagtccc
    17401 cgcactggca atccattccc gggaaaggca tcaccgcaaa ctgctccgga ggcaaccatt
    17461 acagcctctg taacggatta tcagccctgg catcttccga cggcacctcc tgccgccccc
    17521 acgccctgct gtataccccg gtaccgcagc gtgggcagca cttttccccc tcataatctt
    17581 cgtcacacat cacacaaaac cactttctca ccttcaccgt ataatccgga accgtcatgc
    17641 cctgcagatg caaacgacgg gcctctttct ctgccgggtc aagccaggta catcgcagca
    17701 ggattttttc agcgacagtc atctggtcat gttcaaacca cggtggttgt ccgtcattgc
    17761 cccgctgaag tgttaacggg catccacagg acacacagca caaaacgtcc tgtcggttgt
    17821 gcttcgcagc ttctttcgcc gtaacaaaat ggcctttgct gtcattcgcc atataacagt
    17881 tcagaattcg catatttcct cctgagttgt ccggggaata ctgaatcctc cggtctggca
    17941 gtgtgtgcag tactgctccc cataataatc gtggtgacat tgtctgcagt gccagctggc
    18001 tttacgcacc acgggtaagg catccggtac gaattgctgc agacgtttaa tcaactgtat
    18061 ttctctgcgc tccggcctga cataagggca ctgttgaccg tgctctgtca gcctgtcgtc
    18121 agtgtgttca aaccagggaa gttcagtttc gtattgcgga tggtatctga gtgcactgcc
    18181 acacaagtgg caggtgtagt tagcataagg tgcggcctgt gcagtacggg cgcccgtcag
    18241 acgtccgttg ccatcaagag cgataaagga ttttgcgtac atagtcatat acctcagccg
    18301 ccagacgaca cgcaggcaac agccgtcccg gtacgggcag cgtggtcagg gtgtgaatgg
    18361 cggttcagtt aaaatctgga ctggagtttg ggtaaagcag gctggttgct gttacagcac
    18421 tgtggttgtc acacaatgga acactgacgt aaaaaacagt cagtgggcga gtggatacat
    18481 cgttgccgcc agacaacaac ccatactttt ggtttgccgg atattggcgc gccagtcgtg
    18541 ctcaactccg gctacagcca aaattgcgct caccggtgca gtgagctttg gtatgctcct
    18601 tcgccagata gtcagcacgt tccagcactt gctgaaagcc ggtgtcatca ttacgcgcca
    18661 gccacaccgc cggccaggcc ggaaaatgca ccagcgtggc atggcacccg acatccactc
    18721 ccagggcact gcaccacgcc tgtttaatca tcccggccag agactccgga gcgcggtaat
    18781 caccggcacg acaccaggta tcccggttga ccagcagcat caggtgatag tgttttttac
    18841 ctttgcactc cccaaactcc cgggcccaga cgtaatgcag ggttgtggga tgcacgcgtt
    18901 taccttcccg atgcttacgt ttctggtaag cgtcgattcg ggctttcagg gcattgatga
    18961 agcgggatat cacagctgcg tccgttgctg ccggtacatc cgggagacgc aggtcaaccc
    19021 gcaatgccgt caggcgggga tgaacattca gtgcgtgccg caccgtctca cgaatacgtt
    19081 gctgccagaa ggggttgtat ttgtaggtca tggttaaatc tccgtatggt tcatacggaa
    19141 tagccacgtc gtaaaaaata cgcagagtcc ctgaagtgac ctccggcagg acactgtctc
    19201 aggcactctg tgataacccg aacggtcgtc tccggactga cggttgaatt ttcagcgtcg
    19261 ttttttaatg taaaaaaccg gttacgggta aggctgtgag gagggagtga tggtgatacg
    19321 caaaaagaag tgcagggact gtggaaacgc gattactcat aatacagtgt gttgcccgta
    19381 ttgtggttct gtcgacccct tcggctacta ccggaataca gaccgtatcg ttaccatact
    19441 gctggcgctg attattgtgg tcctgctgac tactgtcagc gtcagtgtgt gtatactgtg
    19501 tagctggtga gataacggtt catgtatccc ctgagctgac agtgtaccgt gtatattaag
    19561 cgtaaccgac aggttctcct tcataccata ttaaattttc cagggattgc cgctgcctta
    19621 tttcgtccca tacaggaaaa ctcatctggg tttctgacaa atacgcgaaa ctcacaaccc
    19681 ctcaatatca accacacgtt gtaagccggt aatacctgaa aatcagccta ttaccgccca
    19741 cgcctccggc accattccct tttgatttcg aattctttgt tgtcgctata ccgcttaatg
    19801 ttcccggtcc tccggtgcag ataacaccgg agtcatcgta gcgctgcttc aatattcagg
    19861 tgacgtattt ccagacggaa gccggcgcgc gatcgtaaag tttgttcatt gtcagttctg
    19921 ccagccggtt gtcaacggca gagttaaagg cttccagctc cgcaagggta agtttataac
    19981 gcgtgtggga attcactttt cccgcgtctc ctgacgtatt acgggctgcc acttacctgg
    20041 tgtcccgcaa tacagcctta gtgttccgtt atcggggatc cggacgcacc gcaacaaccg
    20101 gggaagtatt tctggtttag agcagtgtca agagggattt ctcagcccct cactcaccgg
    20161 ctcagtctca gggcaggagc attaatacgc tcagttcatg tcttatacag gaatgggcac
    20221 agggggagct ggcgtgtcac tgctgatttt acgcatcagc gttcatgtat ctgcactctg
    20281 atacatgaac gattcacagc gctctgttac cggagagatt tactctctta gcttactctg
    20341 gcaaatcctt tacgttactc tctgatgact tcttatattt ataataccaa tcagacagcc
    20401 ttatcccatc
//
LOCUS       DBKELA010000079        20288 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.079, whole genome shotgun sequence.
ACCESSION   DBKELA010000079 DBKELA010000000
VERSION     DBKELA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.079"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="ACUQR0_002781"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="ACUQR0_002781"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HHV9109736.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="ACUQR0_002782"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="ACUQR0_002782"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HHV9109737.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="ACUQR0_002783"
     CDS             complement(1727..2998)
                     /locus_tag="ACUQR0_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109738.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="ACUQR0_002784"
     CDS             complement(3181..4386)
                     /locus_tag="ACUQR0_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109739.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="ACUQR0_002785"
     CDS             complement(4486..4842)
                     /locus_tag="ACUQR0_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HHV9109740.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="ACUQR0_002786"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACUQR0_002786"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="ACUQR0_002787"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="ACUQR0_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HHV9109741.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /gene="ydiJ"
                     /locus_tag="ACUQR0_002788"
     CDS             6772..9828
                     /gene="ydiJ"
                     /locus_tag="ACUQR0_002788"
                     /EC_number="1.1.99.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1903457 - lactate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyglutarate dehydrogenase YdiJ"
                     /protein_id="HHV9109742.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="ACUQR0_002789"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="ACUQR0_002789"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HHV9109743.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="ACUQR0_002790"
     CDS             10335..10523
                     /locus_tag="ACUQR0_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HHV9109744.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="ACUQR0_002791"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="ACUQR0_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HHV9109745.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="ACUQR0_002792"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="ACUQR0_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HHV9109746.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="ACUQR0_002793"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="ACUQR0_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HHV9109747.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="ACUQR0_002794"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="ACUQR0_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HHV9109748.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="ACUQR0_002795"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="ACUQR0_002795"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HHV9109749.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLIAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="ACUQR0_002796"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="ACUQR0_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HHV9109750.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="ACUQR0_002797"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="ACUQR0_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HHV9109751.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRIAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="ACUQR0_002798"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="ACUQR0_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HHV9109752.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="ACUQR0_002799"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="ACUQR0_002799"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HHV9109753.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19766..19960)
                     /locus_tag="ACUQR0_002800"
     CDS             complement(19766..19960)
                     /locus_tag="ACUQR0_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109754.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5317 a         4854 c         5175 g         4942 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggt
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaata gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcatc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgata
//
LOCUS       DBKELA010000080        20158 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.080, whole genome shotgun sequence.
ACCESSION   DBKELA010000080 DBKELA010000000
VERSION     DBKELA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.080"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..720)
                     /locus_tag="ACUQR0_002801"
     CDS             complement(<1..720)
                     /locus_tag="ACUQR0_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp53-like domain-containing protein"
                     /protein_id="HHV9109755.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGF"
     gene            complement(720..1400)
                     /locus_tag="ACUQR0_002802"
     CDS             complement(720..1400)
                     /locus_tag="ACUQR0_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HHV9109756.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(1397..2596)
                     /locus_tag="ACUQR0_002803"
     CDS             complement(1397..2596)
                     /locus_tag="ACUQR0_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HHV9109757.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(2597..2950)
                     /locus_tag="ACUQR0_002804"
     CDS             complement(2597..2950)
                     /locus_tag="ACUQR0_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109758.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(2950..3690)
                     /locus_tag="ACUQR0_002805"
     CDS             complement(2950..3690)
                     /locus_tag="ACUQR0_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HHV9109759.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            3913..4203
                     /locus_tag="ACUQR0_002806"
     CDS             3913..4203
                     /locus_tag="ACUQR0_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HHV9109760.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(4227..4571)
                     /locus_tag="ACUQR0_002807"
     CDS             complement(4227..4571)
                     /locus_tag="ACUQR0_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109761.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(4584..5648)
                     /locus_tag="ACUQR0_002808"
     CDS             complement(4584..5648)
                     /locus_tag="ACUQR0_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109762.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(5651..5956)
                     /locus_tag="ACUQR0_002809"
     CDS             complement(5651..5956)
                     /locus_tag="ACUQR0_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate plug family protein"
                     /protein_id="HHV9109763.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(5958..6560)
                     /locus_tag="ACUQR0_002810"
     CDS             complement(5958..6560)
                     /locus_tag="ACUQR0_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HHV9109764.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(6560..8575)
                     /locus_tag="ACUQR0_002811"
     CDS             complement(6560..8575)
                     /locus_tag="ACUQR0_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HHV9109765.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(8565..8756)
                     /locus_tag="ACUQR0_002812"
     CDS             complement(8565..8756)
                     /locus_tag="ACUQR0_002812"
                     /inference="COORDINATES: protein motif:HMM:NF047033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6889 family protein"
                     /protein_id="HHV9109766.1"
                     /translation="MTFNSLPGGEDFILRPALAFHIDQKDLNSGAVDLCRIALLNDYL
                     DMREDNDARVDKWRAANER"
     gene            complement(8753..9178)
                     /locus_tag="ACUQR0_002813"
     CDS             complement(8753..9178)
                     /locus_tag="ACUQR0_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HHV9109767.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(9182..9622)
                     /locus_tag="ACUQR0_002814"
     CDS             complement(9182..9622)
                     /locus_tag="ACUQR0_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HHV9109768.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(9633..10778)
                     /locus_tag="ACUQR0_002815"
     CDS             complement(9633..10778)
                     /locus_tag="ACUQR0_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HHV9109769.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(10782..11324)
                     /locus_tag="ACUQR0_002816"
     CDS             complement(10782..11324)
                     /locus_tag="ACUQR0_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage neck terminator protein"
                     /protein_id="HHV9109770.1"
                     /translation="MSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGLSAGMV
                     RPCWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECMASFYG
                     PAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTVRLRRK
                     VVREYGIKSLVEAPVIFFGD"
     gene            complement(11314..11703)
                     /locus_tag="ACUQR0_002817"
     CDS             complement(11314..11703)
                     /locus_tag="ACUQR0_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HHV9109771.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(11690..12244)
                     /locus_tag="ACUQR0_002818"
     CDS             complement(11690..12244)
                     /locus_tag="ACUQR0_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109772.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(12241..12648)
                     /locus_tag="ACUQR0_002819"
     CDS             complement(12241..12648)
                     /locus_tag="ACUQR0_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HHV9109773.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            complement(12614..12835)
                     /locus_tag="ACUQR0_002820"
     CDS             complement(12614..12835)
                     /locus_tag="ACUQR0_002820"
                     /inference="COORDINATES: protein motif:HMM:NF047914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY1053 family phage-associated protein"
                     /protein_id="HHV9109774.1"
                     /translation="MAKNNAVIHVHTPFVLTLPDGSRREFVKGRHAVEEDVATHWFTR
                     AHAEVSVGKATDARNEVKNAKESKPASGK"
     gene            complement(12878..13819)
                     /locus_tag="ACUQR0_002821"
     CDS             complement(12878..13819)
                     /locus_tag="ACUQR0_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HHV9109775.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(13831..14334)
                     /locus_tag="ACUQR0_002822"
     CDS             complement(13831..14334)
                     /locus_tag="ACUQR0_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="structural cement protein Gp24"
                     /protein_id="HHV9109776.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(14339..15559)
                     /locus_tag="ACUQR0_002823"
     CDS             complement(14339..15559)
                     /locus_tag="ACUQR0_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HHV9109777.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(15574..16182)
                     /locus_tag="ACUQR0_002824"
     CDS             complement(15574..16182)
                     /locus_tag="ACUQR0_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HHV9109778.1"
                     /translation="MDALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAE
                     LRNMVDRAPVGQVMKSIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAK
                     EIAASGDVSRSRANLIARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREM
                     EGKFVEWGKPPTLDGMTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(16199..17665)
                     /locus_tag="ACUQR0_002825"
     CDS             complement(16199..17665)
                     /locus_tag="ACUQR0_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HHV9109779.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(17665..19287)
                     /locus_tag="ACUQR0_002826"
     CDS             complement(17665..19287)
                     /locus_tag="ACUQR0_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HHV9109780.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(19290..19844)
                     /locus_tag="ACUQR0_002827"
     CDS             complement(19290..19844)
                     /locus_tag="ACUQR0_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HHV9109781.1"
                     /translation="MKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARATGSENLS
                     KPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIKDWPKVW
                     RTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADG
                     GPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(19846..>20158)
                     /locus_tag="ACUQR0_002828"
     CDS             complement(19846..>20158)
                     /locus_tag="ACUQR0_002828"
                     /inference="COORDINATES: protein motif:HMM:NF024784.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHV9109782.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         4496 a         5642 c         5081 g         4939 t
ORIGIN      
        1 aaatccagtg ttatggacat ttgtcgccga tacagtcggc tgccctgacg cgccagtaac
       61 gttcaatgta actgtgacag caaggactct tgagaatgtc atcgggaacc tcatcccgta
      121 tgttgaagat acattcgggg agccattcaa ctgaccgcac tgtaaaatta atccagacgg
      181 aaatttttgc gcagcagccg ttgcggtatt acttgacgcg aacaggctca tatcaggtat
      241 ctgattcgcc cctgtaccta cattcctttt agccgcttct cccaaaccaa ggtatgtgag
      301 aatgtcagca atagtatttt tcccaataat gtcacggcca acagaagtta aatcagtctg
      361 cgctgctgta tcatttccag tgaaatatgg gagtttattt gcacctgttg cgagcccagc
      421 taatgccgac agcgtggcat caagcgcctg gaaatctttc ccgaaagcgg tccccatttt
      481 ggatataaac ccgttcaggt ctccatcatc aagcacatcc agcctgcttt tgttggcggt
      541 gtactgcgcc aacgctgccg cgataaagct ggcttgccga atagctttgt tgacctgtgc
      601 gctggatgct ttacctgccg taaaccctga aagcagagcc ggaagtgctt cccagtcagc
      661 ttgtgaggtg acgttagcgt tcggatcaag cgcgaaaggt ttaaagttgt ttattgccat
      721 tagagtattg tcccccatgc tccaacatcg aacccgccga tgtattcgtt atccatatca
      781 aacccaaaga atttagagcc ctcggatggt gcttctaccg aaggcgtttc aacatcaccg
      841 gcccatacgc cagctgcttt aacggtgaga tagccctgtt tgatagcggc aatcagttca
      901 agagacacat cagaaatatc agtctcgggg aatacccaaa ccgatatcgt catgtcctgg
      961 ttgtcgacaa tttgcatcct caggcctgag ccttcagtcg cagcgtcaag gatgggaggc
     1021 agagagtcgt tccgaccgtc ccagttgttg atagcgattt tcgctttcag aatgatgcgg
     1081 tacgtctcat cgcttagcgt cgtatagcca gaatcaggat catatggccc tttccagatg
     1141 ccctggtcat acccaagccc gtcagtgtcc cagctgaaat aaactccgct aattggctgg
     1201 ctgactatgc gactgcgtcc gatccacaga ccgaggatgt cgagctgtac accgacagca
     1261 gtatcaatat cgaaggctgt tataagccct gacatagtgc tggacacatc aatcagcggg
     1321 cgggtgctca gatctatatg gtcaaaaaag agtggcttgg tagcgtggta gttagtgatc
     1381 agttcggtgt atttgctcat gaggtcaccg tgatactgat attcgcggtg ctacaggacg
     1441 cagaagcatc ataggcaata tcaatgtttg atgccgatac gctgccagac gacttaccga
     1501 tcagcaggtc ggtaatatcg taatagcggg cattcccgcc gctcacaacg ccgaggtttg
     1561 ccggggaata aatacggctc agcagaacgt cgtcgccaat tgttaggcca tttatataat
     1621 cggcaacagc ctgtttaatc tgctcgccga tttgagaggt atagccggta aaaactttca
     1681 gggtaatggc tacgaaaatt ggcacatcgg tagagcgcga aaaactgatg acgtgtggat
     1741 taccgtaagt atccggcacc gtgacagaag ttttaccgta agttgcggtt ccctgccctt
     1801 tactcccccg gattgtttgg gctatctcgg taacatcccc tccatcgacg atggcggaaa
     1861 tagagtgtgg cggcagcccg ttgctgtcgg ttgccccagt gtcgttctca tatagtttgt
     1921 gacgtgtcac gccagcaaca ttagcgatag caccgtcgac accttcaaac ggtgtgatcg
     1981 atggtagcgc gacgctttgc ccctgccgaa tgcgcagctc tgcgtcggtt tcggctggtg
     2041 aaccgacagt agccgcaact ggattggtta ccgacaccca acctcgggtc ggggtgttga
     2101 tagtggtaat agtcccggcc agcgccgcaa ccgaaccgct attcgcacat gtggccgtca
     2161 ccagcacagt accatcaacg ccgatcgcta cacttgcggg aaaattccag ataatgccgt
     2221 ttttatcccg tgcggagcca ttcgtgatag tcgtgcctgc cgtaccggtt aacagaaggt
     2281 cagcagtaga gtttgtcgct acttttcgcg tgatcccgtt aattttcaca ttgctgctaa
     2341 gcgctgcggc ctgcgctgtc gtcggtgaaa aagagttgta gatcccgata gcggtgttgt
     2401 tagcgtcatg cacggcaaga gccaccagcg cgaccatctg cccgtctttg ctgtctggtt
     2461 cgaggtaggc atcactaccg taaatctgcc tgaaatagct ggtcagtgta tctaggattg
     2521 tctggtaatc aggcgcacta atcccctggg cggttaccgt tgccgatagc cccagcgtgt
     2581 cgaggttcaa agccatttat gcctcgcttg ttacagtcgt ctggccgtag attgtgtcaa
     2641 tggaggaagt gaaggtgacg cgacggctgg tgccgtcata attggtatcg aaggaaagaa
     2701 tcgacagaac gcccggtgta tcctgtatgc gttcgcgtat agccaggatg tagacatctg
     2761 atcgctgctt cccaagcact gactgaacat acggcgtgcc ttccgtcaga tcgagaaacc
     2821 actgaccgcg ccacagctcg aaacgggttt ttacggcctg ggcgacacat tccggactgt
     2881 cgataaggaa agtatcgtca ccctggccga aagtgtaatc gccttcagta tcttcgcgac
     2941 ggtatcgcat tattgcggcc ctccggtagt tcccccgcca gtctgtactc cgccatgttt
     3001 atgcgtggcg acactgatac cagaagctgt tacgtcattc gttaccatca ccggcccaag
     3061 catcgtcgca gtaccgccgc tttcgcccat tccctgagac aagttgccat taatcgttac
     3121 gttgccgttc agcgtgatag tcggggatgt gattgtcgtt ccaccttcag ccgtagctgt
     3181 aagctggccc ggtgttttaa tggtgatgtt atgtcctgcg gcgacttcta caaacgccgc
     3241 accatcatcg gttcgcagct gcgcggcgct ggtactgata ccgctgattt tccgtgcttg
     3301 cgactgcggg ccaacgatgg cgaacgcatc agataagtca tgctggcgcg ggtcgacggt
     3361 ctcctgaacg ccgccgctct gccaccaaaa atcgatgcaa cggtcagcaa aaatcaggag
     3421 gcactcgtcg ccttctttta ccggaaaggt cagcgtgcaa ccgccgccgc gcgggaagat
     3481 gaccggcaca tccaccagcg gttttaattc ggtggatcca tcgccaacaa taccgcgtag
     3541 cgccacctct actgtgcagg taacagtctc aggatcgaac gactgaatga tgccgggcat
     3601 cgctacgcgc atctgggtag acaccgaatc ggcaatggcc tgcgcggtct gctgctcacc
     3661 gccgatctgt gattgagttg gaattggcat aaaaccccca taaaaaaacc agccgaagct
     3721 ggtttggtat caattatctc ttagtaacta agctacatca gtgtgaagta aggcttttcc
     3781 gctgcaatgg cacacattca caaggtgtat taggatatgt ttgcaacaag taagcattga
     3841 aaatcaattt cagccatgaa gccgccatag agtggacctg caccatcgca ttattgacta
     3901 caggagtaac aaatgggatt tagatttcgt aaaagaatcc ggattgcgcc cggactcgcg
     3961 atcaacatta gtaaaagcgg agtaagcact tcaattggtg gtaaaggttc caccattaac
     4021 atcgggaaaa aaggcgtaaa gatgacaaat ggccttcccg gcacggggct gtcgcatacc
     4081 actaaccttt actctcccgg gaaatcgaca gaaaaaaaac aacttactca taagcaaaaa
     4141 ataattagaa acatcctgtt cgtaattatt gtgtttataa taattaaagc tcaatatttt
     4201 tgacgcatgc ccgcctatct ggcgggctat tttacttttc ggcagtcgta tgttgcatac
     4261 tgacgcggcg aatccatgct ggcttgcagc cactgtgcgt tgagaatggc tttgccgttt
     4321 cgattgatgt actcaagacc aacccatctt ccaggctgat cggtagccat acgccaatcc
     4381 atcttgatat tgttataatc gcctttgttt ttcaggaagg tgattttttg catttctggc
     4441 tttgcgccat tgattctggc taagccatca tctgcccaat ggattttaaa atcaccacat
     4501 tgctgatccg caaaagcggg tgctgatata agcaccgcga acacggtaac agtgcaaagt
     4561 aaatgtctca cggccccacc tcactaattc gtcgtccctt gcattgcttt cgggctataa
     4621 agatcacgag cgccacgcgc aaaacacatc aaatccatgt accacgcctg acctctggtg
     4681 tcgccagtat agtcgatagc tttgacgata taaacgccat ccgtcgcaat gctggcagcc
     4741 tgtgacgtcg tgccggtcag cacacggttg ccgttctctt ctgtttcggt gatacgcccg
     4801 ggcgactgtg ctatttcgct attgccgagc gtggcgcggt acaccgaagc ctgatcgagc
     4861 tggataagac cattaatacg gatgtttggg tttatcaggc atcgcacgtt tactccgcca
     4921 cccatcgttt gttgcggcat accgatcagg ccagtattgg cattcaacac aatggcttcg
     4981 tgaatatatt tatcctccgg caccatctgg acctgaccat ccaccagttg ccatgtcgct
     5041 ttgcactgcg cagcaatatt atccatcacg ttgcggctgg atgagtaaat cgcgcggcca
     5101 cgaggaaaca cggtatcagg aaaatcgccg gtaatgccct gtgtcacgcc gaacgcgttg
     5161 aaatcctgca tcgtcgcccg gtgcagatcc gcaacggtat atccagcggc aagcgtggtg
     5221 atggtggtcg catagaggaa cgcttcgtga tcaccaatgg cctgaatcaa cacccaggaa
     5281 tccgtaatgt tgtccttccc ggtgacggtg aagcgaatat caccgtcaaa aatcaggccg
     5341 tagttctgac cgttcacctg ccctatctgg tctggtgaaa tctcccggga cacaccaacc
     5401 tggctcgcat caacatccgg cgctataccg tcatacccgg caatgatgcg aattttggca
     5461 aactcctgcc caagtatctt gttcgtggta tcggtcgaaa ggttgtaaat tttcacgttc
     5521 gccacgcgcg gccagcgtgt gtctgcccac tcgatctgga acgtgacctt aaagtcagac
     5581 agggaaacgc cctgcccgtt ctggtccaac agttgcagct caaaatggcg catccagtta
     5641 agagacattt ctactcctgt acgaaaatga ggtggctgta tgtgccgagg ttggttttgg
     5701 tgggctcgtc tggtgcgccc ttatcggtcg ccaccaccag cgcgccatca atgccaagct
     5761 gtggatattg tcgtaaaagg tttacgccgg tcagtagagg tacgccagag agaagcgcgg
     5821 caccgccgct atccatcacg tccataatcc agccagccgc atcacgccag atgatcctga
     5881 gtgtatacgt ggtattgccc agcaaaactc ggaactgctg attgtcagga gaaagcggta
     5941 tttcgttaaa ctgcatatca tcccccgaat gctgatgtaa cgcttccgcc cagctggctc
     6001 agcaaggatt cgtttggcgg tgtagtggat ttcatcccgg aattctgcac cgccgatgtg
     6061 ctgacgccat cctgcatatc tgatttatct gcaacgctaa cgctctgcgt ctgcgacatg
     6121 atcacttcac gcagggtaag cgtgcagttc agcacgttct cgctggtttt atccgttgtc
     6181 acctcgatgg ctcgcaccag catattgctg taaacccgct ttccggtcac tacatcgaac
     6241 ggcaccctag aggactggag atccagtagt tgctgatagg tctctttcgg gctaagtccg
     6301 gcgctgaggc cgattgacga tgtatcaatg aagtccagca acgaaccgcc acctgcgaag
     6361 ccgcattcca tcgtgacttc gctggggcgc ttatacgcat gatcggcgat gaaacccgac
     6421 gcgctattcg ttgttggctt ctccaccggg tgctcagtaa tttcgagcgc atcagaatgc
     6481 ttttcagaaa caaccacgct ggggattaat attccaattc gccgggattg ctggcgaaaa
     6541 attgctgata aaatatccat tatctcggtc ctgcggggag ttgctgggtt aactgtgaat
     6601 tcacgccctt ttgacggtca acagtcaaac gggcagcctc gcgcggatcg aaaacgccgt
     6661 ggatgttaat attcgtttcc tgctgaatca ccggggcgct ggtgggcata ttgctcatta
     6721 ctttcggaat gtagttgcgc gtttcctgcg gcattagccc cattccataa cgcttaacat
     6781 tcccgatccc ccagttatat gatgccagtg ctttgctaag gtctccgccg ttctgccgca
     6841 acagctggct caggtactta gcggctgcct gagctgactt ttccgggtcg aatacatcat
     6901 tcccacgcag gcccatgtcg cgcgccgtgc catccataaa ctgaaacaga cctttagcgc
     6961 cagcgcctga aacggcgaac tgattaccgc ccgattccgt tatggccacg ctgcgcaata
     7021 acccctccgg aagccggtag agctgttcca ggttggtaaa catcggctgc atccatccca
     7081 gcagttcaga gcctgctttg gttggctgtg gccgcttaac tgactggccg agctgctcag
     7141 gctcatcatc accgaaccag ccgcgcaccg ttcggcccac gctgcgagga tcgaatcccc
     7201 agtgctcttt aatccagtcg gcggttccgt tggcgctgtc tgttaccatc ggcatcgctg
     7261 acggattttc gctgccctga ttaagcatct gtttgccgat gctggcggca tcagcccagc
     7321 ggccatcttt aatggcgttg agcaggtcgg cgatcatgtt cagcatcttg ccgaactcac
     7381 ccatttgcga aatgaagttg ctaaaatccc actttaggga ccatgacttg gggtcaatac
     7441 cgagcaactt agccagcgct tttgtcaggt ccgtaacagt ctttttcagg tcaccaatca
     7501 ttttgacggc ctgatcaatt tcagtcttcc atttccccca gtcaatcagg cttttgccac
     7561 cttctttcca cgtctggtaa tcatccaaga gcagaccaag tgcaataata agcgtcgtta
     7621 tcatgcccaa gggtgatgac atgaaagcgg tatttagcag ccgccatgca accaacagcc
     7681 cactgaacaa tgcgataagc tgctgcgtgg ctgggttcag ctttttgaac caggcgatca
     7741 caccctcaac agcttgccca gttcgccata acacgcgggt aatcgcgtca cctgcccaga
     7801 gaattccttt gatgatcttc gtgatgaccg cttcaatctt cggccagttg tcgagtatct
     7861 gcctgcggaa gttatcaata ctgccagcaa ggccaccagc taggttagag ccaatcttgt
     7921 ctttcgcctg tccaagcgtc atcgtcagat tacgcatgga ggtcatgaaa atattggact
     7981 gtttagccgc tgactcagca ttaaaaccaa tacgctttgc cgtcaacgcg tactcagaac
     8041 tgagctgccc catccctctg cgcatcgcca tcagcgtgtt ttcatcgatg ccgagcatct
     8101 gcgcgtattg cttcgcgcgg taatacggca tgttgttgag cttttggcca acgccagtaa
     8161 agatggcagc agtatcacgc atctttccgc tggcatcgcg ggtctggaca ccaagacggt
     8221 tcaggaaccc ttccgccccc ggattgctac gcatgaagcc agccaaccct tcgagggagg
     8281 acatagccga ctcggcgctg gcaccggttt gcgatgcggc gtatcccagc gctttgatgc
     8341 cctggacact ggcccccgtc cgctgggatg cccagtaaat tttatccaga ccgttcgcga
     8401 tctgagtggt aaatccgaca atgctcagcg ctgcgccttt caccaccgcg ccgaccttca
     8461 gaacgttcgc ggtaacgcct ttcagcacgg cttcaaactt attagcgcca gcctgatcga
     8521 tatcgaatcc cagcgaaaca aggaaatctt taatcgtatc tgcgttaccg ctcattggcc
     8581 gctctccatt tatctacccg ggcgtcgtta tcctcgcgca tgtcgaggta gtcattgaga
     8641 agcgcgatgc ggcaaagatc taccgcaccg ctgttaaggt ctttctggtc aatatggaag
     8701 gcaagcgccg ggcgaagaat aaaatcttca ccgcccggca ggctgttgaa ggttattccg
     8761 ctagcggggt gggcgtctcg ctggtaggga gtccttgcaa aaaatttccc agcgagtcgg
     8821 cgaccacccg cgccaccagt tgcagcatgg taagcaggtc gatatcgtca aacgccattt
     8881 cgccatgctg gcagaccggc acccagcctt tcatgtgctc gcgtgaaaca acggaaagac
     8941 aggggaacag gatagcgtcc acgtcgccat cactcagatc ggacacagca ttggcaatct
     9001 ttggcaggat agtagccatc gcgccttcgg tgtctttgct gctgatcttc tcctgaacgc
     9061 tccggaagtc cgaaaccatc ccggccagaa ccggcaacag cttgcgggac accttcagct
     9121 gttcgaaaac gctgagcttt gcggtgcgat atttcacgcc tttaatttcg aattccatgc
     9181 gttaaaactc cccgagaagc tggtcaatct tgccgcagtc gaatacccag gcgacggttc
     9241 cgccctcttt agcgttattg aaatcaggct gtttctggaa tgcacacgaa cgcgcagtag
     9301 aaatatcacc cgatgccgtg ttgcgaatga cgatcacgtt attgccccag gtggcagagg
     9361 actggctttg cgcgttatac gccagagaca gctttttatt caccggggag gttttgagta
     9421 gcgtcaccgt aatggtgcct gacttatcgg cgtgcaggct gtgcatcacc tcgccatcgg
     9481 caccgatagt catggtgttt ttgttgccgc ccatggtctg ggtgatacct tcctcagagt
     9541 tcgcagaacc ctgaccaaga tcgataacgc cggtcggccc ggtgagcgac gcggttacat
     9601 cgagaaaaga ataagttgcc atttatcgct ccttagcgaa ccacgttgat ctgcacatcg
     9661 gcataatgaa ctgcgccagc cagcttacag gccacctgga ttaacggtgc tttgcgcgct
     9721 tcacggtcgg cctgcgcctg ttcggacagc ggttgcgcat acacgtaata gcctttggtc
     9781 agcgtatcgc cggaattcag ctgtccgata gggccaccat tccacacgcc agcagctacc
     9841 agaccgttcg tgacggactg atccatggac tgttcaacgt tggaaagcag acgggtcaca
     9901 ccggcatcag tctgcggaat tttggtggtg ctggtgtaaa gcaggttata gaggttggtc
     9961 tgaacgtagt tctgcaacca gtcgagcccg tggcgctcgt cgaagaagtc accgttcgcc
    10021 atgacaccct gttgcaggat cgccgtgtcg ttggcgtagt acacgaatac attgcagttt
    10081 tttgcatcaa gtgccgatgc ctggctgact gtcagtgttt catacccgac acccggctcc
    10141 tgcttaaact tgagcgtaat cgcggtatta ctgccattga aattaaccgt gaatgcccgg
    10201 ccaaatgcag ataacgcagc gtatttatta cccgatgaat attgaataaa actgcgtgaa
    10261 tatccggcgg ttttcagttt tgatgccaaa tcatcgctgg atgcagtctg caggcatttc
    10321 tcatcgcttg tcgtaatcgc cagaatacgg cttacagaag aggattcgat cgccgcagcc
    10381 actttcagcc agtctgcatc cggaatatct tcatcgtctg caatccccag cccataccat
    10441 gaagtataat cgagcatggc attcacagcc tgctccagcg tctcaggcgt ggcctgttcg
    10501 ctgtctccct tcgttttcac ccaacgacca acaaaaacct cctgaggttt cggtgattgt
    10561 gagaaaaaca cctgcgcagc cttatattct ggtgattcca cgccaaaatc ttttccaata
    10621 tcttccgcgg cagaataacg gcgaatgcgc tcacttaccg gaatgattgt ggacgggccg
    10681 agaatgagta atgcaccaaa atttcgccct gatgctgcac gcggcgacat gatcacatca
    10741 acattaacaa cgtttgatac aggcaagccc tgtgccatag cttaatctcc gaaaaagatg
    10801 actggtgctt ccaccagcga tttaataccg tactcgcgca caaccttccg gcgcagacgc
    10861 accgtcatat cgtagcggcg gacccattgc tgattaataa gttcagggaa gagacctgtg
    10921 taatcgccaa gagacagccc cagcgcattc agtgctgcgt tgttctgcgg tacagatata
    10981 ccgtcacgaa accgggacgc atacaccatc cccgccggac cataaaacga agccatacac
    11041 tcaatcgttt catgccgcca gagctgagag ccatcatcgg tctgtctggt gaatgccgga
    11101 ctgtcatcac ctgaccatcc gataacccca aacgcacacc agttcgtttc aaccggtagc
    11161 agtggcggct gctctttctg ccagcacggg cgaaccatcc cggcagacag accggaaacg
    11221 ttacgcatcc actggcttaa cagcctgtcg agcgcttcgt cataatccgg atcgccactg
    11281 gttggtatta accatccgcg ctctgtactg gtgttattgc tcaaccggag ttcccccatc
    11341 aaacggcatc aactcacaat gcgcctgaac gaatccggcc ccataagctg tatacgggtc
    11401 gacgaaggtc acacgataat cacggccctg atacgtcacg atatcggcat cacggccagt
    11461 ctgtccctgc gtcagtcgct cagtcgtcac aatcagaatt gcaccgctga ttacctgccc
    11521 tgcctgcata cggcggtttt ccagagagcg atcaacagtt acgactccgg caaactgctt
    11581 tttaacttca ctgtcgctgc cgatcccgtc ctcatccacc gtttgcactc ggcgtgttac
    11641 ccacaaattg aagtcgcaaa aatcggggtc aaaaagcaca tctgttacat caagagtcgg
    11701 catctttatc cctcacaaca tgggtaatag ctctgcgata ttgcccggtg tcaattaatg
    11761 gtttcgccag atcggttccg ggagattcgc cagcaacacg ccgagcaagt tccagtgttg
    11821 cccccttgcg cccccgacga gcccgggctt caacggtgct atcagcaagc ggtgtaaagc
    11881 cggtaatggt catgtaacgc ctgacgccat tcgcggccag cgttccggcg cggttaagcg
    11941 ctctttccgc acccgccgca tttccatcaa gcgcagcctg cgccgctgct ttgagctgcg
    12001 gcaccgtctg ttcctctacg gatttaacgc cggggatcag gtgcgggcgt gggggtatgt
    12061 tttgcgctgg tgagccgtat tcgttgacgt aaccgatccc ggcattacca aacggaacat
    12121 cctcacgctc gctgtcttct tccgggatgc ccaccagcac atcctttttg gttagcgacc
    12181 ggagcgcatc cagaatggcc tgagcgttat caaccctcgt tgttacacca cttttgaaac
    12241 tcatagctgg cgaccgcccg caccgaacat cgtgatcagc tgataaaatt cagcgccata
    12301 tcgggtgtta ttccagaagc ctgcgtcagg gtttagcgtc gcgctggtgt catagctgac
    12361 gcttaccttg tcaacggact tggaggactg aacaccattg gttgaaccgc ccgggccgcc
    12421 aaccagcatt gcccgactat ctgccgccca tagcgtcatg tagtgagcca cgaacaaccc
    12481 ggcaaagtac ggaaacaaat ctttgccggt gacgttttcg ctcagcagtt catcggccag
    12541 attcagacga aactggattt gcgcttcggg atatttggca gggtcagcaa actgcgggaa
    12601 gtcgcggcga aaatcactta ccgctggcag gctttgattc tttggcattt tttacctcgt
    12661 tacgcgcgtc tgtggctttg ccaacggata cttccgcgtg cgcacgagtg aaccagtgcg
    12721 tggcaacgtc ttcctccact gcatgacggc ctttaacaaa ctcgcgccgc gaaccgtcgg
    12781 gaagcgtgag cacaaacggg gtatgtacgt gtattactgc attatttttt gccatcgggt
    12841 catccttaat ggccccgcca ggggggccat gtggctgtta aatgccatca acgtacgaaa
    12901 tggtttcttt gtacactggc tcaaccgcac ccagcttgcc gtagtaggtc gcaatctgat
    12961 acagaccgcg atactggata ggaacgctct gcaacggcac cagcggatag cggacgtatt
    13021 ttttatcgtt ggtgtacgca accatgcgat ccttattccc cacaccacgg cctttcagcc
    13081 atttaaccgc gcggatattc agcggaacac cgttctggtg atagctgatg gtgttggtct
    13141 gaaggtacgt caacagggac tggttacccg cagatgaaac gatgatgctg gacaacagag
    13201 caaactgctc tggcgggatc agcaaatcac gcggaaccac agagtaaccg gaagcggccc
    13261 acgcatcaga cagcacctgg ttaatgcttg cgcggatttc gtccggtgtt gagattgccc
    13321 acgttttggc agcgttgttg acaggcacgc cgtccagggt aacaaggcct ttcaggttta
    13381 atgcggaatc gccaacatac acctgctcgt cgttgtccat ctgccatttg agctgcatcc
    13441 cgtcgtactt ctgggtgtca atcgggcgac ctacctgctg agctgctgcc agctctacaa
    13501 cggtccagcc cagttccatg ccccagagat tcagcgggtt accggatttg ccgatatcca
    13561 cgtttacgcc agcaatagcg gttgagtctt tgcctaccca gtttttgcca ttcggatttg
    13621 caccagtacc cgcagcggcg aagctggtat tcgtccagct ggaaatgtca tctgcgatag
    13681 agacatcttc acgcaactga atatcgcggg tccaggtgta ccccgccagt ggcaggttca
    13741 gagtctggtc gagtcgctcc agctctccga tgagaaaggc accagagctg tcaacggttg
    13801 cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc caggaaattt ctgcattgcc
    13861 gttagcatca ccggcacctg tgaattcagc gttggtcagc accacatttt tgccactgac
    13921 tgacgtggac atgaatccac ccagcggcac tttgatggat tcatcagtgg agacgacaac
    13981 gtataccggg tcgccttttt tgatggtgct ggcatcaaaa tcagaaccga gattaacggt
    14041 cacgtagcca cgcttcatgg cgtcgcccgg gaagttcttg cctgttccca cctggcgaac
    14101 catgtccggc tgcgaagtgg tcggataagg gcgcacgtag atccccttca ccttgtctgc
    14161 ggtatcgcca tctgccagcg gcacgaaaaa accgtcagca tcgtatttac cagccagccc
    14221 ataggcagca aaggcgttat cggatttaag gaccaccggt tcgacggtta agtcctgcgg
    14281 gcgagagata gccccggcaa tgccaacagg catccggtac agatatgcag tcattggatt
    14341 atcctttgcg gttagaccag aattcagcgt tttgtttgtt cagggaagcg atgctggtca
    14401 tgcccatgtt tggacgttgt gcatcgcccg tggtgctgcg ggtgtttcgc cctttggcaa
    14461 tctcagacac agcgttaaac gccatatcga ccgattgctt aggcaatttg cggatatccg
    14521 catcaccgac aacctggcga accagcgttt tgtcagcaga agccagaacc tcgcgtttga
    14581 acacggtcgg tttcatctta cggctcagat cgatacccgg aacgataact tcggcacgcc
    14641 aggctgagtc accagtaatc gtggtttcct cttcatcgtc ctcgccgtca ccggtcggat
    14701 tatcgtcagg tttattatcg ttatcgcccg tggcatttcc ttccagctta gccagcaggg
    14761 ctttcagtaa tgttttgagg tcatcatcac tgtcgccggt tggacctccg cccatctctg
    14821 gtactttgtc cggtagcggt tgctgcgggg acaggttgat gttgagatta acgccctgcg
    14881 gcaaatcccc ctcatctcct gtaaccgatg cgggagccga ctccaccagt tcgttcatgg
    14941 tgtcagcgtc acccgttttg atggccgtgc gcatgcgggt ccaccagctt ttcttttgat
    15001 ttgccattgt gtctctgtct ccaattgcac aacgatttcc ggctctgcct ttggggacaa
    15061 gagccacatg gtttccggta atatcgacct gctcggcttt acctggctcg gtctgctcgt
    15121 actccgcgtc atagccgcac gacacttcac gcaggccatc ttcgataagc tgaatggcgt
    15181 tttcgtcttt gacgataagg tcagccagca tcaaatcaga gtggtcaccc gtcccgcgcc
    15241 gcacattctg aagatgcccg acagcaagct ctttccagtt ctcgggattt accagccgca
    15301 cattcccgtt ttcatcttca ggatgcagga tcgtgatgct catcccttcg aatgaggcga
    15361 gcgtggccgg atggaatacc tgctcaggag agcgcgttac gactatctca ccgagcttgt
    15421 cgggtttgag gtttggcaga tcggcagcgc cgtagagctg tttacccgtt cgacctatcg
    15481 gcacgtcttt gcacagcagc gagccgtcag ccagctgata gcgggtttcc cccagccggg
    15541 tattgaaaaa atatttcatg gtttacctgc gattcaggcg agataagaat gagggttggg
    15601 aaaaacgatt tctttataac agcgacaatt cgggagctcg ccagcgtgac cggtcatgcc
    15661 atcaagcgtt ggaggtttgc cccattcgac aaacttacct tccatctccc gatgagaatg
    15721 ccggacgtcg ccatcttcgg ctgtacgcca gatataacca ttcgagccga ttgacagcgc
    15781 acgcgcctga tcgagcgcgc cggttgcacg tccaagctcg gtacgggcga taaggttcgc
    15841 tcgtgagcgt gacacgtcac cggacgctgc tatctctttc gcgaatggtt cagcgcggcc
    15901 accagccaca acggcctcga tggccttgtt ctgaatgtca tacacccgat cggcggcctc
    15961 aagaggcagt gacttgatat acttaatttg ctcggcgacg atggatttca tcacctggcc
    16021 taccggggcg cggtcgacca tgttgcgcag ctctgcgctg atgttccggc tgtgctgacg
    16081 ccactgcttt tcattctggc gtgcaatgtc ggcggtaaag ttctcagcaa ccttcgtcgc
    16141 ccagggggtg atgatttcgc tgtagcgctc cagcgcatcc attatttcgg taacgctatc
    16201 atttgaacca tcgtagcgcc catttacgat atccccgacc gcccgcgcta tctgccgtag
    16261 gctcgttcga tatcggatct ccgcctggcg gctctggcgg tttgtcgcca agttcgccga
    16321 tgcctggcgg cgcttcgtct tcggcattct cgatatcctc gtcggtaatg gatgccccga
    16381 tgccggtgac gtcagagttt tcgcgcaggt cggtcatcgc cgccttacgc gtcatcaatc
    16441 cgtcgcccag cgcggtacta atcgcgttgg tggtgtttac ggccaccgtt gatcggtcaa
    16501 cgtctgacat ttgccatagc gggttaaact caaacgtgaa atcgtccggc agcggctttc
    16561 cgagttccga gcggtgcata atgtccagta tccggcgcat cggcagccgt aagcggcgct
    16621 cctgcaatga gctcacccgg tcgtaatagt tggcgaggtc tgcatcacca gtagagaagc
    16681 ctttcgggga ttgaccgaac aaccgcacca gcgggatacc aacggcaccg ctaatctgct
    16741 cagcaaactg cgaaagaatg tcatccagac cactaaagct gtactggtgg gtttcgaact
    16801 tatcccgcga gtccatgagc gtcatacctt cattgctctg gaactggcgg atcaggtcga
    16861 tgttcttcag caacgcctcg aacgccgggc ctccaagcgc gataagctcg cgcaacttct
    16921 ccacgctata ggtacgcaga tgtgctttgt agaccagctg cgccgcgccg acagtggcgc
    16981 tgtcgaacgc agtaagccga tcccagatac gctctacaac cgacattccc cattcgtttt
    17041 cggtcatctt ctgctggaat ggcagcgtca ccccgtcgaa gcgaatcagg cggctgtgat
    17101 ggatgcgcca ggccgggatg cccgttgcag tggtcaccac gtcgtaaaac tcaggtttgc
    17161 cgagatctgg ccccatctct ttaatgcggc gggtcaggac cgggttaatc atccagcggt
    17221 cgagcgggag aatgccctta aacttgcctt ctccaatggt ttcgagccgc agcggggtca
    17281 ttggtgcctg cccctcgatc atgatgaagc cgaccgcgcc gccgtagagg cgagaccatt
    17341 tcagcacgtc gttcagcgca tcccagattt gcaactcatc cagttgtgat tcgagaatgc
    17401 cacgatcttt tgcatcaatt tccgaagtga tgcgaatgcc tttgcgggtc atatcatccg
    17461 ggatagcatc gaccgcttcg ccgataatcc aggacgaacg ataggaccat tccaccagca
    17521 tgcggttacg actggtgaaa ttagcccggt aggtggatgc tgagtgctgg ttaggtgtct
    17581 gcatccccac gcgggcaaca aagttctcat agccatcagc ggtggcctgt gccgttcgcc
    17641 gcgtggcttg tttgtttcgt gccatcaggc ctgtctccct agcagctccc agatgttcag
    17701 ggctgaattc attggcgcgt agctgatcat caccgagtcg gcaaggtttg gcgaccgggt
    17761 tccatcaggc tgtttatcaa taacgatttt tcccacacca ttaatggaat aggtcggctg
    17821 cgaaagctcg atgatgagtt tatctttgag tgccatgcta ctgctgattg agatgatttc
    17881 gtccgggttg taatccatac cttcaaccac ggcgcgccag gtattctgaa aaagtttacg
    17941 taaccgccac cagctctggg ctttggcgtt agcgaagaag tccttgttca ggcgggcggc
    18001 ctgtccgttg tcaccgcgca ccgcttcgtc gtccggatca aataccgcgc cactacctcg
    18061 aaacggtgtg gcgagtattg acggtcggcg cgcagcgtta cgcagttcgt tgatagcgcg
    18121 tgcatcgccg cgaacgccag cgcccaaccc gtcctcgtca aagcgaaact cttcgaggtt
    18181 gtcctgttcg caaaagccga aaaccttctc gacggactga taaatgtcgc tgcccacacc
    18241 ggaccattcc cgcacattct ccaggaggaa gccatgacgg gtggaaaagg aatttttgtc
    18301 cctgccttcg tcggcgacgt ccatcgcgcc cagtcgcttg cctgttggct ggatgcccag
    18361 cttgatatgc gcgtcgacag cagcctgtac ccattcggat ggaatcagga cgccttccgc
    18421 tgatgcactg tagttcaggt caagttcctg tgccaccacc accggattgt cgattttctc
    18481 gcattccctg cgataccact cttcatcctt gcgaggatca tcccgccagt ggaatgtgaa
    18541 taccggtatc ttcccgccat gacgcttctg agcgaacggg ttcgccatgc cgttaactga
    18601 actcaggtca atacggcaac gcgtcgtttg tgacaacgcc gcatcaatca gcagaggacg
    18661 ctgaaggaat gcagactcat caaccagata aagcgtggta cggtcaccac ggccaatatt
    18721 atcgccagcc tcacctttga taacggcacc agtttcagga aactcaacgc gcatgtatgg
    18781 tgcgtgcttc ttttcgtccc acgaacctcg aaactctaca ggtagtgttt ccacgaactt
    18841 gcgcgccttc cagaacaatg ctttcgggtc accggtgctg tcgacgtatt cctctttacg
    18901 ggagccgaaa ccgataacca tttctttgtt gaagagacaa agcgagcatg ccagcccgat
    18961 cgcggtccaa ctgagcccca tttcacggga tttttcggta ataccattct cccgattacc
    19021 ccagcgttcc ataatccagt ggatccactc ctcctgctta gggaagagta aaaacggaat
    19081 ggtcaccggc aggccataat caatattacg cgggtccgtt gtcatgcccc agtcgatgat
    19141 gaactgagcc ggattggttc ggtaaaactg ttttagtgca ggcaatattt caggattctg
    19201 gcgaatgcgc tgtaggcgtt ccatccgcca ttcaaaaacc atctggtaat caggatgttt
    19261 aaaatcgaag gggaatggta acggcatact tagcccatca tttttctata cgcctctgca
    19321 gcctgctccg gcgttaagtt ggtaatttct gttctgactg gtcctccgtc agcaccagtc
    19381 acttcatttt tgacattgtc tttaaacgcc tgaacagaaa catgacgccc gagcaactca
    19441 aggtttttaa ccttatcagg ccatttgatt ttcttcagga gagcagcact atctgctgat
    19501 gccatctcga cgacatccat tcctgatagc gttgtacgcc acaccttagg ccagtcttta
    19561 atctgcttca gttcgccatt ggcaagcaga atgtcaagca cgtccatctg atcgatttca
    19621 gtcagccgtc gcaatacata ggcggcgtct atacctacct cttcattgcg ttcagccttt
    19681 agctcagcaa tgcgatctgc tatgtcaggt tttgacaggt tctcgctacc agtggcgcga
    19741 gcagttttct cgctgtaacc cgccctgata gctgcctgag tggcgtttaa atcttttaaa
    19801 tattccctgc agaacagttc ctgcttaggt gtgagctgtt tcaaatcaca cctccgaaac
    19861 attgctccaa tttctatgat taacgatgtc agcaatcatg gtagtggata cgccatatct
    19921 ctttgctaaa gacctaagcc cattacctac actcccgcgc acatactcgc ggcgaatgca
    19981 gatgacatcg cactctttta atttcgccat tccgttcttt tcacctgaac ggtcagggta
    20041 tgagtttctg cctcgccttg acctgtcacg attattgtca gcgacagtac ctataatcag
    20101 atggtcagga ttcacgcatg atggattatc gcatttatgc ctaacaacaa ggtccttg
//
LOCUS       DBKELA010000081        19883 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.081, whole genome shotgun sequence.
ACCESSION   DBKELA010000081 DBKELA010000000
VERSION     DBKELA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.081"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            3..659
                     /gene="pdxH"
                     /locus_tag="ACUQR0_002829"
     CDS             3..659
                     /gene="pdxH"
                     /locus_tag="ACUQR0_002829"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HHV9109783.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            788..2062
                     /gene="tyrS"
                     /locus_tag="ACUQR0_002830"
     CDS             788..2062
                     /gene="tyrS"
                     /locus_tag="ACUQR0_002830"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HHV9109784.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2121..2984
                     /gene="pdxY"
                     /locus_tag="ACUQR0_002831"
     CDS             2121..2984
                     /gene="pdxY"
                     /locus_tag="ACUQR0_002831"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HHV9109785.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3028..3633)
                     /gene="gstA"
                     /locus_tag="ACUQR0_002832"
     CDS             complement(3028..3633)
                     /gene="gstA"
                     /locus_tag="ACUQR0_002832"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HHV9109786.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3739..5241)
                     /gene="dtpA"
                     /locus_tag="ACUQR0_002833"
     CDS             complement(3739..5241)
                     /gene="dtpA"
                     /locus_tag="ACUQR0_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HHV9109787.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(5852..6487)
                     /gene="nth"
                     /locus_tag="ACUQR0_002834"
     CDS             complement(5852..6487)
                     /gene="nth"
                     /locus_tag="ACUQR0_002834"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HHV9109788.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6487..7182)
                     /gene="rsxE"
                     /locus_tag="ACUQR0_002835"
     CDS             complement(6487..7182)
                     /gene="rsxE"
                     /locus_tag="ACUQR0_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HHV9109789.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7186..7806)
                     /gene="rsxG"
                     /locus_tag="ACUQR0_002836"
     CDS             complement(7186..7806)
                     /gene="rsxG"
                     /locus_tag="ACUQR0_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HHV9109790.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7810..8868)
                     /gene="rsxD"
                     /locus_tag="ACUQR0_002837"
     CDS             complement(7810..8868)
                     /gene="rsxD"
                     /locus_tag="ACUQR0_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HHV9109791.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(8869..11091)
                     /gene="rsxC"
                     /locus_tag="ACUQR0_002838"
     CDS             complement(8869..11091)
                     /gene="rsxC"
                     /locus_tag="ACUQR0_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HHV9109792.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11084..11662)
                     /gene="rsxB"
                     /locus_tag="ACUQR0_002839"
     CDS             complement(11084..11662)
                     /gene="rsxB"
                     /locus_tag="ACUQR0_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HHV9109793.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11662..12243)
                     /gene="rsxA"
                     /locus_tag="ACUQR0_002840"
     CDS             complement(11662..12243)
                     /gene="rsxA"
                     /locus_tag="ACUQR0_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HHV9109794.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRALAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12320..12760)
                     /locus_tag="ACUQR0_002841"
     CDS             complement(12320..12760)
                     /locus_tag="ACUQR0_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HHV9109795.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(12846..13061)
                     /gene="ydgT"
                     /locus_tag="ACUQR0_002842"
     CDS             complement(12846..13061)
                     /gene="ydgT"
                     /locus_tag="ACUQR0_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HHV9109796.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13334..13459)
                     /gene="blr"
                     /locus_tag="ACUQR0_002843"
     CDS             complement(13334..13459)
                     /gene="blr"
                     /locus_tag="ACUQR0_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HHV9109797.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13702..14742
                     /locus_tag="ACUQR0_002844"
     CDS             13702..14742
                     /locus_tag="ACUQR0_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HHV9109798.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(14778..15779)
                     /gene="add"
                     /locus_tag="ACUQR0_002845"
     CDS             complement(14778..15779)
                     /gene="add"
                     /locus_tag="ACUQR0_002845"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HHV9109799.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(15883..17055)
                     /gene="malY"
                     /locus_tag="ACUQR0_002846"
     CDS             complement(15883..17055)
                     /gene="malY"
                     /locus_tag="ACUQR0_002846"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HHV9109800.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17065..18657)
                     /gene="malX"
                     /locus_tag="ACUQR0_002847"
     CDS             complement(17065..18657)
                     /gene="malX"
                     /locus_tag="ACUQR0_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HHV9109801.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            18832..19860
                     /gene="malI"
                     /locus_tag="ACUQR0_002848"
     CDS             18832..19860
                     /gene="malI"
                     /locus_tag="ACUQR0_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HHV9109802.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5040 a         5043 c         5119 g         4681 t
ORIGIN      
        1 ccatgtctga taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag
       61 gcgggttacg ccgccgcgat cttcccgccg atccattaac cctttttgaa cgttggctct
      121 ctcaggcttg tgaagccaaa ctggcggacc ctaccgcgat ggtggtcgct accgtggatg
      181 aacatggtca gccttatcag cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg
      241 tgttttacac caacctcggt agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta
      301 gcctgctgtt cccgtggcat acccttgaac gccaggtgat ggtgatcggt aaagcagaac
      361 gactttcgac tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat agccagattg
      421 gtgcatgggt ttcgaagcag tccagtcgca tttctgcccg cggtatcctt gaaagtaaat
      481 tcctggagct gaagcagaag tttcaacagg gcgaagtgcc attgccgagc ttttggggcg
      541 gttttcgcgt cagccttgaa cagattgagt tctggcaggg cggtgagcat cgcctgcatg
      601 accgcttttt gtaccagcgt gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa
      661 aagatgcaaa aatcttgctt taatcgctgg tactcctgat tctggcactt tattctatgt
      721 ctctttcgca tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga
      781 gattttgatg gcaagcagta acttgattaa acaattgcaa gagcgggggc tggtagccca
      841 ggtgacggac gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg cgctctattg
      901 cggcttcgat cctaccgctg acagcttgca tttggggcat cttgttccat tgttatgcct
      961 gaaacgcttc cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct
     1021 gattggcgac ccgagcttca aagctgccga gcgtaagctg aacaccgaag aaactgttca
     1081 ggagtgggtg gacaaaatcc gtaagcaggt tgccccgttc ctcgatttcg actgtggaga
     1141 aaactctgct atcgcggcga acaactatga ctggttcggc aatatgaatg tgctgacctt
     1201 cctgcgcgat attggcaaac acttctccgt taaccagatg atcaacaaag aagcggttaa
     1261 gcagcgtctc aaccgtgaag atcaggggat ttcgttcact gagttttcct acaacctgtt
     1321 gcagggttat gacttcgcct gtctgaacaa acagtacgat gtggtgctgc aaattggtgg
     1381 ttctgaccag tggggtaaca tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa
     1441 tcaggtgttt ggcctgaccg ttccgctgat caccaaagca gacggcacca aatttggtaa
     1501 aactgaaggc ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca aattctacca
     1561 gttctggatc aacactgcgg atgccgacgt ttaccgcttc ctgaagttct tcacctttat
     1621 gagcattgaa gagatcaacg ccctagaaga agaagataaa aacagcggta aagcaccgcg
     1681 cgcccagtat gtactggcgg agcaggtgac tcgtctggtt cacggtgaag aaggtttaca
     1741 ggcggcaaaa cgtattaccg aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc
     1801 ggacttcgaa cagctggcgc aggacggcgt accgatggtt gagatggaaa agggcgcaga
     1861 cctgatgcag gcgctggtcg attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac
     1921 tatcgcctcc aatgccatca ccattaacgg tgaaaaacag tccgatcctg aatacttctt
     1981 taaagaagaa gatcgtctgt ttggtcgttt taccttactg cgtcgcggca aaaagaatta
     2041 ctgtctgatt tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa atcactcccc
     2101 ctggttttta tacagggaac atgatgaaaa atattctcgc tatccagtct cacgttgttt
     2161 atggtcatgc gggtaacagt gcggcagagt ttccgatgcg ccgcctgggc gcgaacgtct
     2221 ggccgctgaa caccgttcaa ttttctaatc acacccaata cggcaaatgg actggctgcg
     2281 tgatgccgcc cagccattta accgaaattg tgcaaggcat tgccgccatt gataaattac
     2341 acacctgtga tgccgtatta agtggctatc tgggatcggc ggagcagggt gaacatatcc
     2401 tcggtatcgt ccgtcaggtg aaagcagcga atccgcaggc gaaatatttt tgcgatccgg
     2461 taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtgc
     2521 ggcacggttt gcctgccagc gatatcattg cgccgaatct ggttgagctg gaaatactct
     2581 gtgagcatgc ggtaaataac gtcgaagaag cggttctggc agcgcgcgaa ctcattgagc
     2641 aagggccaca aattgtgttg gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt
     2701 ttgaaatgct gctggtcacc gccgatgaag tctggcatat cagccgtcca ctggtggatt
     2761 ttggtatgcg ccagccggta ggtgttggtg atgtgacgag cggtttactg ctggtgaaac
     2821 tgcttcaggg ggcaacgctg caggaggccc tggaacatgt gaccgctgca gtctacgaaa
     2881 tcatggtgac caccaaagca atgcaggaat atgagctgca agtggtggct gctcaggatc
     2941 gtattgccaa accagaacat tacttcagcg caacaaagct ctgaaatatc tcatttcggc
     3001 ccacaacgac ctgtgggccg atggctatta ctttaagcct tccgctgaca gcgcgtcttg
     3061 tacttccgga cgttcagcca tacgttgcat aaatgccgca atgtgctcta acccttccag
     3121 attcagtttc accgcgtatg cccagcgcag aaccgtaaac agataggcat cagcaattgt
     3181 aaatctttgc ccgcagatcc agtgctcatc cttcagtgcc tcgttcacat attgcagctt
     3241 cttatccagc tgcgcgcgaa ctgtcggttt gtactcttcc ggtgtatctg ggcgaaacag
     3301 aggtgtgaaa cctttatgca gctcggtggc gatgtaattc agccattcga tggttttata
     3361 gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg gggacgctgt cggcaagata
     3421 ctgcataatc gcaacgcctt ccgtcagcaa agtaccatcg tccagcagca atgcaggcac
     3481 ctgcccctta gggttaacgg aaaagtaatc gtcaccgttt tcgagacgtt ttttcattaa
     3541 atccacactg acgagcgtaa aatcctttcc gctctcacgc agggtgatat gggaagcgag
     3601 agagcaggcg cccggtttgt agaacaattt catcggtaac tccttttgtc tgtggtttaa
     3661 agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta
     3721 aaagagtttc cctgaatttt acgctacagt agctttcgcc gctttgtctg cagcgtcatc
     3781 ctgcgtcatg cggtgcagtt taggcgcggt cagcagcatc agtactgcaa taacggcagt
     3841 agcgacacca atctgcaaga atactcgacc atagacttcc agtgacatca gcgggtcggt
     3901 aacgttatcc ggcacagcca tcatacccgc aacataacca ccaatcaggt ttgcaccggc
     3961 agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc
     4021 aaccattgcc agacccagac cagagatcat cagttccccg atgctctgca ggccatagct
     4081 tgcgaccagc cagctgacag acacgatacc tgcgtcagac gcgaatttcg cacccagcgg
     4141 cagaatcagg aacgcaccag aacacatcac catgccgatt gcaaacttgg ttggcatcgg
     4201 cagggtatca cccatcttgt tatagatagc ggccagaatc ggactaccga tgatgatcca
     4261 gaacgggttc agtgcctgat actgctcagg ttctacggcc agacccagaa tggagtgctc
     4321 aacgttacga atcgcaaaga agttcagtga cgttggcatc tggctgtaca gcacgaagaa
     4381 gatgattgct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt
     4441 catcgcgaaa gcttctttac cgaagataac cacaataccg aaggcaacaa cgcccagcgc
     4501 catacgcgca acttcctgat tgtgcagtag ccaggtggca acagcgatca gcgccacaac
     4561 accaataatg gtcagcaaca ggttacggta gttgactggt tcaaagtctg gttttgaacc
     4621 gtactgttta acccagcgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac
     4681 aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg gccagccacg gggtggctat
     4741 catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag
     4801 acgtgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag ctttaaacag
     4861 gccgttaccg accgcaatag ccgccatacc catataaaca ataccggcgt cgtgaccaga
     4921 ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt
     4981 agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc
     5041 actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag
     5101 gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga
     5161 gaagatgaga tagaacgcct tcggttgttt gaaagcgttc aaactgacgc tttcagttgg
     5221 tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta acgggggtgc
     5281 tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca
     5341 ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg ttacatataa
     5401 ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa
     5461 cgtaacggca gattttttca ctgcattgag agggggatga tggcttggcg gcgatatgtt
     5521 ctgctattta gcgtttttcc tagtgagata gtccagtttc tgaaaaatag ccagtgtaat
     5581 gttttgcagg tcaatatttg ttgcgtgtgt aaatttaaat cattttaacc tgattcttgc
     5641 aatgttttta cattgccaca gcatcgtgat cttgatcacg aatgaataga aaatttcgct
     5701 attaaaaaaa caattaacct gcatttttgg ttatccgcaa acggattacc tgatgataac
     5761 gctaagcaat ggcatcattg acgttaaaaa gatgttttaa aagaggataa agaaaggtta
     5821 tcaatggggt aatcggtgtt accccttttc ttcagatgtc aactttctct ttgtattcac
     5881 aaagatcttc aataatacaa gagccacagc ggggcttgcg ggcaatgcag gtataacgcc
     5941 cgtgcaggat caaccaatgg tggcagtcga ctttaaactc tgccggaacc actttcagta
     6001 gcttttcttc tacctgttcg acgtttttcc ccggcgcaaa ttgagtacga ttacaaacgc
     6061 ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgaa tgcagtgttt aatacgacgt
     6121 tggctgtttt acgacctacg ccgggcaggg cttcaagcgc agcacgatct tccggaacct
     6181 cgccattatg ttgttccagt aatatacggc aggttttgat gatattttct gctttgctgt
     6241 tataaaggcc aatcgttttg atataggttt tcaccccttc aacgcctagt tcaagcatcg
     6301 ctgcaggcgt attcgccacc gggtagagtt tcgccgtcgc cttattaaca ctgatatcgg
     6361 tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa aggcgaactg aaattaagct
     6421 cggtggtggg atgaggattg ttctcacgca ggcgagtgag gatctccaga cgttttgctt
     6481 tgttcatcag acattccctg tttcaccgtt tggcaatgca cgttcggcag ctgcttcagc
     6541 acggcgcttt ttcattcttt catcaatcag gtattttcct gccagcatca gtcccaagcc
     6601 aataaatgca cctggtggca gcatcgccag caggaaaggg gagtcggtgt ggaaaatctc
     6661 cacgcgtaat acttttgccc agctacctaa cagcgcatct gcaccgtcaa acaacgtgcc
     6721 attgccgata atttcgcgta gtgaacccag cacgaacatg gcgcaggttg cgcccgtacc
     6781 aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga aggcttcagc
     6841 gcggcccaca acgatacagt tagtgacaat cagcggaata aaaatcccta atgattgata
     6901 caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg ctgaccaccg aggcgatgat
     6961 catcacgtaa atgggaatgc ggatctcggc tggcgtccag tgacgcagcg tcgaaatggt
     7021 caggttggtc agcgtcagta ccagcgtagt cgcaagtcct aaacccagag cgttagtggc
     7081 agtggatgtg accgccaata gcggacaaag gccgagcagt tggaccagtg cagagttgtt
     7141 tttccacaac ccctgaacaa taacgtcttt aatttcgctc acgctttatt ctccacaggc
     7201 aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc
     7261 attaaccacc gcgcggggag taatcgtcgc gccggtgaac tggtcgaaat caccaccatc
     7321 tttcttcacc gcccagtgcg catcatccgc accactgatt ttttttcccg caaaatgggt
     7381 gagccagtca gaaaggcgca attcgatttt atcgccaagc cctggcgttt cgtggtgctc
     7441 tgtcacgcgc gtgccaagta ccgtgccatt aaaatcggct cccaccagca gctgaatcgc
     7501 accggaatag ccatctggcg cggttgcttc cagaacggcg gctaccggtt tgtcatcctg
     7561 tttggcgatg taaacccgat gctcaccttt acctaactct ggcgcagtta ccagatagca
     7621 actctgtgcg agcgcattgt tatagcgttc ggctggcagc acctgatcaa ataacgtctt
     7681 ttgttgcaga ctggcctgtt cagcaattgt cgttttggtc atctggttga tggccgcagt
     7741 taaccctgta gaacccgctg caaatagtgc cagtgtaatg ccgtgttttc ggatagtttt
     7801 cagcatggtt taccctttgc gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag
     7861 aggaaccgtg atgttcgcca gcaggacggc aaaagccacg ccgtcaggat agccgccgaa
     7921 actgcggatc aaccagacta ataagcccgc cagcgcgccg aacatcagcc gaccacgatt
     7981 ggtcgtagaa gcagtaactg ggtcagtcaa aataaagaat gcgccgagca tagtcgcacc
     8041 agacagcaga tgaatttgcg gtgctgccag tgtttctggt gagaacaacc agcccaacgt
     8101 tgcgcataac gccagcgtta ctaagaagct gaggggaata tgccagcgaa tcgctttctg
     8161 ccatagcaac cacacgccac cagccagcca ggcgagattt acccattgcc aaccagcgcc
     8221 cgccagaata ccgctgtaga tcggatattg cataatctgt tcaaccgaat gacctgcgcg
     8281 gacagaggtt ttgaatgtat ccagcggtgt cgcctgacta atgccatcaa tacctaagcg
     8341 tagcgtattc atatcaccac cactggcggt atgaccgctg aaaataacct gtatggcgtc
     8401 gataaaacca gggatgttga ccgcaatttc atgtggcggt aaccagctgg tcatctgcac
     8461 cgggaaggag atcagtaaga ccacataacc aatcattgcc ggattaaacg ggttttgccc
     8521 cagaccgcca tacaactgtt tagcgatgat caccgcaaac accgtaccca gtacgaccat
     8581 ccaccatggc gctagggggg gaatacttac cgccagcaat aagcctgtca gcaatgctga
     8641 gttatctttc aacgttgcgg ctaccgactg cttgcgtagt ttgagtacga gagcttcggc
     8701 taacagagca ctgaccgacg ccaacaggat ctgaacgaga gtaccccaac caaaaaacca
     8761 cagttgcgct gcgattcctg gcacggctgc gagcaacacc aacagcataa tgcgcgatgt
     8821 ctggcgctgg ttatgggtat aaggggagct agctattctg aataccattt agtcctcgtt
     8881 tacaaccttt tgctgggcgg cttttttggc ctgaacgcgg gcaatagccg cggcaactgc
     8941 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
     9001 gcgtgctttg gcgcgggcaa tagcggcttc gacggcagct ttgcgcggat caacctgttc
     9061 ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggcgc gggcaatagc
     9121 cgcttcgacg gcagctttgc gcggatcaac ctgttcttct ggctccgcat tggcctgttg
     9181 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcgg ctttgcgcgg
     9241 atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc gcgctttggc
     9301 acgggcaata gcggcttcga cggcggcttt gcgcggatcg acctgttctt ctggttccgc
     9361 attagcctgt tgctgttcca gcttgcgcgc tttggagcgg gcgatagctg cttcaacggc
     9421 agttttacgt ggttcagcaa cggttgctgc gtcgttagtt tgctccagtt ctgcctgttt
     9481 cgctctggct tgcgctttac gggcttcccg ttctgcaata attgcactgt tatccgggcg
     9541 ttcgcccgct ttaatcacaa taggctgtgt agcctgggcc tgtttctctt tcacccgcgc
     9601 cagagcggca gcaatcgcat ctttatcttt ggctgcaggt tgaacggctg cgctcttatg
     9661 tcgttcaagg cgagtcgctt tttcgcgctc cagacgagcc tggcgcgctt cgaaacgcgc
     9721 tttggcttct gcggcgcgct tttcttcctg acgaatagcc gcaatttcag ctttttcctg
     9781 acggaaatat tgcaccaagg gaatattgct cgggcaaacc cacgcgcaag ccccacattc
     9841 aatgcagtcg gcaatgttat gcgtggtagc tttatcgtgt tgctgacctt tgctgaacca
     9901 gtacaactgt tgcggcaaaa gatccacagg gcaggcgtca gcacaggcgc tacaccggat
     9961 gcagctttgt tcttcctgtg gttcgccaag ttcattggca gagggagcca acagacagtt
    10021 ggtaatcttt acgaccggga catccagcca tggcaaggta aagcccatta gcgggccacc
    10081 cataatcacc atttgatcgg cagaggggca gaatccggca tcattcaata aatgacgcac
    10141 tggcgtcccc agccgtgccc agacgttgcc cgggcgagcg attgcttcgc cagtcagggt
    10201 tacaacacgc tcggtaatcg gctcgccatc aataacggca cgtttcactg cataagcagt
    10261 gccgacgttt tgcattaata cgccgatatc ggatgaacgc ccgccatgtg gaacctgctt
    10321 cccggtcaga atgtaggtta attgtttagc accgccagaa ggatatttgg ttggaatcac
    10381 ccgcagagaa atatcgttag agtccgccag caccgcgcgc agcatggaaa tcgcctgcgg
    10441 tttgttatct tcaatgccga taagaatttc gcgtggctgc agaatatgcg caagaatgcg
    10501 aataccctct acgacctgag ccgcgcaatc ctgcatcaaa cggtcatcgg cggtaatgta
    10561 cggctcgcac tcagcagcgt tgataatcaa cgtttcaatc ttatctccac caccctgcaa
    10621 tttaacgcct gtcgggaatc cggcaccgcc cagcccggca acaccaaact gatgtatgcg
    10681 ctcgattaac tcttcgcgac tgcgagagcg ataatcgacc cagccgtcgc gcgggatcca
    10741 gcagtcttca ccatcggcat caataatcac gcttaattca gctaaagctg aaggatgagc
    10801 cgtagagtgg ggcgcaatag ccgtaacggt acccgaggtg ggcgcgtgaa caggcagcat
    10861 tttgccgcga ccacgggtaa gcggctggcc gcgcaatact ttatcgccga cgctaacgca
    10921 caactcacct tcagcgccaa tatgctgttt cagtggaata acaaaatgct gcgccagtag
    10981 tacctggcgc aggggggtac cgttggtctg ggttttcatc tccggtggat ggatgccgcc
    11041 gttgaaatcc cagattttat tttttctgaa tgcagagaat aacttaagca tggtgttcca
    11101 cgggaatgat acgcacggga atggtgttca gatcccattt ccaggagtca ggtgtttctg
    11161 cgaccggttg caacgagatg cagtgcgtcg ggcacggatc aacacataaa ttgcagcccg
    11221 tacagagatc actcattacc gtatgcatga cacgggtagc gccaacgatg gcgtctaccg
    11281 gacaagcctg aatacattta gtgcagccaa tacagttatt ttcatcaata accgccacca
    11341 tccgcgcagg cgttagctct tgcgcttcgc catccagcgg ctgcggctcg acattaagca
    11401 actcggcaat ttttagcatc acagcttcgc cacctggggc gcaacggttg attttttcac
    11461 cgttacagct gatggcttcc gcgtaggggc gacagccggg ataaccgcac tgaccacact
    11521 ggctctgcgg taagatttcg tcaattttct caacgaccgg atcgtcttcc accgcaaaac
    11581 ggcgggaggc ataacccaga atggcgccaa acgccaggcc cagcaggctc acggcggcaa
    11641 cggcaatcca gatagcattc attacaactt caccaaacca ctaaagccca taaaggccag
    11701 agacataaga cctgcggtaa ttaacgcaat ggcattaccg cgaaaaggtg ccgggacatc
    11761 agccacagca aggcgttcgc ggatggcggc gaagagcacc atcaccagcg agaaaccgac
    11821 agcggcggaa aaaccgtaca gcgccgactg caagaaattg tgcccgagat tgatattcag
    11881 caacgccacg ccgagcactg cacagttggt ggtgataagc ggcaaaaaaa tccccagcag
    11941 acggtaaagc accgggctgg ttttgcgcac caccatctcg gtgaactgca cgaccacagc
    12001 aatcaccaga ataaatgcca gggcgcgcag gtaaatcaga ttaagtggga tcaaaatcca
    12061 cgtatcgata agccaggcgc aaatagacgt cagcgtcatc acaaacgttg ttgccagccc
    12121 catgcccatc gcggtttcca gttttttgga aacccccata aacggacaga gaccgagaaa
    12181 cttgaccagt acaaagttat tgaccagtac agttccgaca aacagtaaca ggtagtcagt
    12241 catttttcgg cctgaaataa aaaagccgcc tattatccgt taatccagag caggcgacaa
    12301 caggttaact gtaaggttat tacggattca caaacgtcgc ttttacccgc gacgagcgtt
    12361 tgaaataggg gacgatcaat gcggttgcca acaaagtaaa cagcaactgg cgaacggcta
    12421 tgccaacttc cacgggtgaa aaagcgaatg cttttactgc cagcaatacg gaaataagca
    12481 gccagataat atagtgttta ggaacacaac gccgacgttt aaagaaagcg atggtcaacc
    12541 acagtgtgta ataccacatg gcgatagcgg taataaaaga aacgccccat aagatttgtg
    12601 tcgtcagtgc ttgtccgccg agcgtttgaa atgtttgcgg agaagataac gcagcggtat
    12661 acagcaacaa cgccagcgtc gtactcaaca gagcgactaa aagccaggcc aacgggccaa
    12721 gcaaccagcc tccaatacgt tgtggcgttg tggtggtcat aggttctcca gaatagcgac
    12781 gcaaacaggc aaatctaatt ttaggggcgt tattataagg cttttataac cgatcgctac
    12841 tttctttatt ggacatagtg ccagacggac ttcggtacct ggccgaggtc aaacaaacgc
    12901 ccgccagaaa ccagctctgc gcgacggtga tcggcagcac gatacatatt gatcagttcc
    12961 tgatcgtccg tcagggtgta attaagatgg tcgtagagtt tttccagact ttcgagtgaa
    13021 ctgattttgc gaaattttaa taagtagtcc tgaacagtca taaaaggtcc ataaaataat
    13081 taataccaaa ggggtaggta ttattagtga tgataaaatt tcaccaaaag tgaacgcttg
    13141 tgttattaca ataataagaa tgcgctgatg gcaaatattc agggatgaaa aactgctctg
    13201 aaaagagtaa cgcgctttac tatccctgac aatcaccaac aacatcgaac aagataataa
    13261 attcctggtt taatatccga caagtgaaaa catgcacccg gacgggcagc atgtcgctcc
    13321 acaagtgcag agcttacttg tgttgtaccg aagcactctg ttcaggtggc tgatagttgt
    13381 caatgtgact cgccacgcca agaagaatga ctgaaacgac aagaacgatc caacctgtta
    13441 attcaataag acgattcatt acagcccaca tctctcttga ttgatccatt aacttcaggg
    13501 ggtaaatgtt acttagcaat aatagctcca gcaagatttt tactgaggtt tttgcgatat
    13561 ttagcttttg tcgttggaaa attcgctatt tttttgactt aagttaaaca acatccctta
    13621 ttgctggcag gttattaaac tgttgagcgt gggtaaagga tagtgtcaaa tagccatcat
    13681 acttcaatga gaggcaatga catgagcgac aacatccgtg ttgggttgat tgggtatggt
    13741 tatgcgagca aaaccttcca tgcgcccctg attgcgggca cgcccgggct ggaactggcg
    13801 gtaatctcca gcagtgatga aacaaaagta aaagccgact ggccaacggt tacggttgtc
    13861 tctgagccga agcatctgtt taacgatccc aacatagacc tgattgtcat tcctacaccc
    13921 aacgataccc atttcccgtt agccaaagcg gcgcttgagg cgggtaaata tgtggtcgtt
    13981 gataaaccct ttaccgtgac actgtcacaa gcgcgagagc tggatgcgct ggcaaaaagc
    14041 ctggggcgtg tgctgtctgt attccataac cgtcgttggg atagcgattt cttgacgcta
    14101 aaaggtttgc tcgcggaagg cgtgctgggt gaagttgctt actttgagtc tcattttgac
    14161 cgcttccgtc cgcaggtgcg cgatcgttgg cgtgaacagg gcggcccagg cagcggtatc
    14221 tggtacgatt tagcaccgca tcttcttgat caggccatta cgctatttgg tttaccggtc
    14281 agcatgacgg tagatttggc acagttacgg cccggagcgc agtcgaccga ttatttccac
    14341 gccatcttgt cctatccaca gcggcgagtc attttacacg gtaccatgct ggcagccgct
    14401 gagtcagcac ggtatatcgt gcatggatcc cgaggcagtt atgtgaaata tggcctcgat
    14461 ccacaggaag aacgtctgaa aaatggcgag cgtctgccgc aggaagactg gggctacgat
    14521 atgcgtgatg gcgtacttac ccgcgtggaa ggtgaggaac gtgtcgaaga aacgctgttg
    14581 acggtgcctg ggaattatcc ggcttactat gcggcgattc gtgatgcgtt aaatggcgat
    14641 ggtgaaaatc cggttccggc aagccaggca atccaggtaa tggagttgat tgagctgggc
    14701 atcgaatccg ccaaacatcg cgcgacgttg tgccttgcgt gaaaaatgga tggtgcaatt
    14761 tgcaccatcc atttttgtta cttcgcggcg actttttctc gcagtgcgcg tttttcctct
    14821 gcgctgagga aagccatttc cagaccatta atctgtgcct ggcggatttg ctcacgggat
    14881 aacccagcag ctggcgcggc aacggtatat tcgtgaatga tatccactcc ctgtacgccg
    14941 ggatcgtcag tgttaatgct ggcacgaatg ccatgctcaa ggaacgtttt cagcggatgt
    15001 gcagccagct ctgctacggt gctggtctga atattggagg tcagacagga ttcaatacca
    15061 atttgttgct cggcgagaaa atccatcagc gcccgatctt caatggcttt tacgccatgt
    15121 ccaatacgct ccgcacccag ttcacgaatc gcctgccaga tgctttccgg cccggcagct
    15181 tcgcctgcat ggacggtaat atgccagccc gcatcacgcg cgcggttgaa gtgagaaagg
    15241 aacagacttc ccgggaatcc aagttcatca ccagctaaat caagtgcggt aatctggtca
    15301 cggtgggcta aaaaggcctc cagctcttgc tgacaggcgg cttcgccgaa ggttcggctc
    15361 ataatgccga taagcttcgc ctgcacacca aaggtgcggc aaccttcacg tacgccatcg
    15421 atcaccgctt cgacaacacc cgctacaggc agcttatgtg ccattgccat gtagcctggt
    15481 gaaaaacgca gctcgacata gcgcaggccg ttacgggctg catcttcaat gttttcaaat
    15541 gccacgcggc ggcaggcatc aagagaggcg agaactttaa cgccccagtc aagtttggtc
    15601 agaaagctca ccagatcggg ttcgttggca atgacctgaa cgtggggaat cagtgtttca
    15661 agggattgtg caggaagcga gatattatac tggcggccaa gttcaagaat ggtctgggga
    15721 cgaatgttgc catcaaggtg gcgatggata tcagttaatg gcagggtggt atcaatcatg
    15781 gtcgcactct ttttctggat ataaaagtgc gcaccattat aaaaacaaag cagggtaaaa
    15841 atccacgatt gcgcaacgat tttttacgtt gcgcaattgg ggttagcgaa cagcgcggat
    15901 ggcgtttatt aatccagcca cacctttttc cagtttcgaa cgtgggcagc cggcattgag
    15961 acggacaaaa ccacgacctt cttcaccata ggtatacccc ggcatgatcg cgactttttc
    16021 ttgctcgata agcgcttttt gcaacgcgtt gtcgtcaata ttcaacggac gtagatcaag
    16081 ccaggccaga taagtggatt gcgggatctg ccagttgagt tcaggaaacg cggcgttcat
    16141 tttatctgag atatacgtca ggttatcttt cagatagacg cgtaaggcat ccagccacgg
    16201 tgcaccttgc tgataggcgg cgatatgggc agttaacgcc agtaccgaag gggaagaaag
    16261 cccatcacgt cctttcagtg ccgataaata ggcatcacgg ctgctgctat tttctataat
    16321 cccgtaagca ccggtcagcg cgggaatatt gaaacttttc gatcccgatg tcagcaacgc
    16381 ccagtctccc cgcgccacat tactccaggg aatatgcggc tgctcgcccc agaccatatc
    16441 catatggatt tcatcggaaa taacccgcac atcatgacgc tcgcacaggt cagccatgat
    16501 ctccagctca tcgcacgtcc acactttccc ggtaggattc tgtgggttac acaggagcat
    16561 aattttacat tctggtttcg ccaaaacggc ttccagcttg cccatatcgc aaaaccagcc
    16621 gtcagcctgc ttctctaaag caacgggcat tactgtgcgc tggttacctt caatggcctt
    16681 gtaaaatgcg tcataggcgg gcgtgtggat caccacgcct tcacctgttt cagaccactg
    16741 acgaatcagt tctgaaacca tatagatgac agaagggcca tacaccaccg tctgcgtatc
    16801 aattgcggtg taatgctggg tggaaaacca gtgggcaata gccgcgagaa actcatcgtt
    16861 tttccagcgg ctgtagccaa atacgccgtg catcaggcgc tgattcagcg cctcgataat
    16921 gcagggggca gtggcaaaat ccatgtctga aatcgtgaac ggtaacaggt cagcagtgcc
    16981 gaaacggtca gcgacataat cccactgtgt acaccatgtg ccatgacgat ccacgacctt
    17041 tgaaaaatcg aacatatctt atccttatgc ctggacagta tgcatcagac cggccatttc
    17101 atcttttact gactgaactt gtggcccgat aacaacctgc aggttatgtt gattaagttg
    17161 tactacgcca attgcccgat tgtccttcag tgcctgcaca ttaacaagcg acatatcttc
    17221 cacagacaaa cgcagacggg taatgcagtt atcgaggctg acaatattgt cggcaccgcc
    17281 taatgcttcg aggattgcag gaacgttgta acctgattta cccggcgcac cggcaacggc
    17341 tttttcgatt gagctggcaa cttcgctatc gcgccccggg gttttcagat tgaagcgggt
    17401 gatagcgaaa cggaagatga cgtagtaaac gacaaaccag attgccgcca ccactggcac
    17461 catgtaccac ttggttgaca gaccatgcaa aataccgaac accacgaagt cgatgatatt
    17521 gccgtcggta ttaccgatgg tgacgccgag cacagacatg acggtgaagc cgaggccggt
    17581 taacagcgcg tggatgacat acagaactgg cgctacgaac aggaacagga attccagcgg
    17641 ttcggtagtg ccgccaacga cgcaggcgat caggccagaa atcagcagac ctttaatttt
    17701 atggcgattt tccgggcgcg cgcagtgata catagctaac gctgcacctg gtagaccgcc
    17761 gagaaacgca ggcattttac cttgcgaaag gaaacgcgtg acgctttcag aaaaaccgtg
    17821 agtggtcggg caactcaatt gcgcctggaa gatggtcagt gcgccgctga cggtttgacc
    17881 gcagacctcc tgcgtgccgc ctgcgtcggt aaagcgaatt aatgccacca gaatgtgatg
    17941 cagaccaaac ggcaacagca gacgttcacc ggtaccaaac agcatcggtc cgaaatcacc
    18001 cgcgctgttt atcatatggc ccaagccgct aatacccatg gcgaaaatcg gccagactaa
    18061 tggaatcacc aggccgacaa ggcccatcac cagcgaggag ataattggta cgaagcgcgt
    18121 accgccgaag aatgccagcg catccggcag gcggatatta tggaaacgct catgcagcat
    18181 ccagacgata ataccggcga tcaccgcacc gaggatcccg gtatcgatcg actggatccc
    18241 aagaatgctc tggatgttat tggctttcag aacggcggca tctgtggttg gcagaatgcc
    18301 tttattggtc aaccagaagt ttaccgcgag gttcattacc gcataaccga cgaagccagc
    18361 gaatgccgct acgcctttat tttcgcgtgc caggcccagc gggatggcga tacagaacat
    18421 gacaggcagg aaactaaaag caaacgagcc aatcttactc atccaggtaa agatagcctg
    18481 caacacgggg ttgcccagga ccgggatcag ggttatgaca tcatggctgc taagagaact
    18541 accaatgccg agcataatgc cgcagaacga caataatgcc acgggtaaca tgaaggtttt
    18601 gcctaactgc tggaagaact cccacagcgt gactttcggt gctgttttcg ccgtcataaa
    18661 acgactcctc gtagagaaca atctggattc agtaaattgc gcgagaagat aaaacgtttt
    18721 atcaaatttt agtgagtcat aaatcacatt acgcaacgat aatagcgggt ataagataaa
    18781 taaaaggtaa aacgttttat ctgtcacata atcagggagt aggtcatctg catggctacc
    18841 gccaaaaaaa taaccattca tgatgttgcg ctggctgcgg gcgtgtcggt aagtaccgtt
    18901 tcgctggtgc ttaatggcaa agggcgaatc tctaccgcca caggagaacg cgttaacgcc
    18961 gccattgaag agctgggatt tgtgcgcaat cgacaggcgt cggcgctgcg cggcgggcaa
    19021 agcggcgtca ttggtttgat cgtccgtgat ttatctgcgc cgttttacgc cgaattgacg
    19081 gccggattga cggaagctct ggaagcacag ggacggatgg tttttttgct tcacggcggt
    19141 aaagacggcg agcagctggc acagcggttt tcactgttac tgaatcaggg tgtcgatggt
    19201 gtggtaattg ccggggctgc aggaagcagc gatgacctgc ggcggatggc agaagaaaaa
    19261 gctatcccgg tgattttcgc ttcccgtgcc agttatcttg atgatgttga tacggttcgc
    19321 ccggacaaca tgcaggctgc acagttgttg acggagcatc tcattcgcaa tgggcatcag
    19381 cggatcgcct ggctgggagg gcaaagttcc tcattaaccc gtgcagaacg ggtggggggc
    19441 tattgtgcaa ctctactaaa atttggcctg ccgtttcaca gcgattgggt gttggagtgc
    19501 acttccagcc agaagcaagc cgcggaagct atcacggcgc ttttacgtca taacccgacc
    19561 atcagtgccg tggtttgcta taacgaaact attgcgatgg gggcatggtt tggtttgctg
    19621 aaagcagggc ggcaaagcgg ggaaagcgga gtcgatcgtt actttgagca acaggtttcg
    19681 ctggcggcat ttaccgatgc gacaccaacc acacttgatg atatacctgt tacctgggcc
    19741 agcacgccag cgcgggaact tggtatcaca cttgcggatc gcatgatgca aaaaatcacc
    19801 catgaagaga cgcattcacg caatcttatt attcccgccc ggctcattgc ggcgaaataa
    19861 tcctctcttt atctgctata ctt
//
LOCUS       DBKELA010000082        19460 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.082, whole genome shotgun sequence.
ACCESSION   DBKELA010000082 DBKELA010000000
VERSION     DBKELA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.082"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..131
                     /locus_tag="ACUQR0_002849"
     CDS             <1..131
                     /locus_tag="ACUQR0_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHV9109803.1"
                     /translation="YYTRKMSQGKRHNQALIALARRRCDVLFAMMRDRTFYTPQGS"
     gene            148..2247
                     /gene="pbpC"
                     /locus_tag="ACUQR0_002850"
                     /pseudo
     CDS             148..2247
                     /gene="pbpC"
                     /locus_tag="ACUQR0_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2397..2828
                     /gene="ndk"
                     /locus_tag="ACUQR0_002851"
     CDS             2397..2828
                     /gene="ndk"
                     /locus_tag="ACUQR0_002851"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HHV9109804.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2978..4132
                     /locus_tag="ACUQR0_002852"
     CDS             2978..4132
                     /locus_tag="ACUQR0_002852"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HHV9109805.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4417..5430
                     /gene="rodZ"
                     /locus_tag="ACUQR0_002853"
     CDS             4417..5430
                     /gene="rodZ"
                     /locus_tag="ACUQR0_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HHV9109806.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5457..6584
                     /gene="ispG"
                     /locus_tag="ACUQR0_002854"
                     /gene_synonym="gcpE"
     CDS             5457..6584
                     /gene="ispG"
                     /locus_tag="ACUQR0_002854"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HHV9109807.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6687..7961
                     /gene="hisS"
                     /locus_tag="ACUQR0_002855"
     CDS             6687..7961
                     /gene="hisS"
                     /locus_tag="ACUQR0_002855"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HHV9109808.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7979..8599
                     /gene="yfgM"
                     /locus_tag="ACUQR0_002856"
     CDS             7979..8599
                     /gene="yfgM"
                     /locus_tag="ACUQR0_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HHV9109809.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8610..9788
                     /gene="bamB"
                     /locus_tag="ACUQR0_002857"
     CDS             8610..9788
                     /gene="bamB"
                     /locus_tag="ACUQR0_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HHV9109810.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9906..11378
                     /gene="der"
                     /locus_tag="ACUQR0_002858"
     CDS             9906..11378
                     /gene="der"
                     /locus_tag="ACUQR0_002858"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HHV9109811.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11448..11663
                     /locus_tag="ACUQR0_002859"
     CDS             11448..11663
                     /locus_tag="ACUQR0_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HHV9109812.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11660..13030)
                     /gene="xseA"
                     /locus_tag="ACUQR0_002860"
     CDS             complement(11660..13030)
                     /gene="xseA"
                     /locus_tag="ACUQR0_002860"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HHV9109813.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13192..14658
                     /gene="guaB"
                     /locus_tag="ACUQR0_002861"
     CDS             13192..14658
                     /gene="guaB"
                     /locus_tag="ACUQR0_002861"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HHV9109814.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14727..16304
                     /gene="guaA"
                     /locus_tag="ACUQR0_002862"
     CDS             14727..16304
                     /gene="guaA"
                     /locus_tag="ACUQR0_002862"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HHV9109815.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16399..16938)
                     /locus_tag="ACUQR0_002863"
     CDS             complement(16399..16938)
                     /locus_tag="ACUQR0_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HHV9109816.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16954..17472)
                     /locus_tag="ACUQR0_002864"
     CDS             complement(16954..17472)
                     /locus_tag="ACUQR0_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HHV9109817.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17783..17974)
                     /locus_tag="ACUQR0_002865"
     CDS             complement(17783..17974)
                     /locus_tag="ACUQR0_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HHV9109818.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17992..18144
                     /locus_tag="ACUQR0_002866"
     CDS             17992..18144
                     /locus_tag="ACUQR0_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109819.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18326..>19460
                     /gene="pdeF"
                     /locus_tag="ACUQR0_002867"
     CDS             18326..>19460
                     /gene="pdeF"
                     /locus_tag="ACUQR0_002867"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HHV9109820.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4586 a         4742 c         5287 g         4845 t
ORIGIN      
        1 cttactacac ccgcaaaatg agtcagggaa aacgacacaa tcaggcgctt atcgccctgg
       61 cgagacgacg ctgcgacgtt ctgttcgcca tgatgcgcga caggactttt tataccccgc
      121 aggggtcata acatgcttga caacttaata ggggcacgtt accttgaagc gctgatcaat
      181 tatgaagatc gctggttctg gaagcatccg ggggttaatc cattctcggt ggcgcgcgca
      241 gcatggcaag atctcacttc gggacgggtt atttccggtg gcagcacgct cactatgcag
      301 gttgctcgtc tgcttgatcc tcaccccaaa acatttggtg gcaaaattct ccagctctgg
      361 cgcgcgttgc aactggagtg gcatttgtct aagcgtgaaa ttctgatctt gtatcttaac
      421 cgcgctccgt ttggcggtac gttgcagggg atcggtgcgg caagttgggc ttatctcgga
      481 aaatcgcctg cgaatttaag ctattccgag gcggcaatgc tggcggtgtt accgcaagtg
      541 cccagccgac tgcgcccgga tcgttggccg gagcgtgccg aagccgcgcg taataaagtg
      601 ctcgaacgga tggctgcgca aggtgtgtgg tcccgtgagc aggtaaaaga gtcaagggaa
      661 gaacccatct ggctggcacc ccgacaaatg ccgcaactgg caccgctgtt ttcgcgcatg
      721 atgctcggta aaagcaaaag cgacaaaatc gttactacgc tggatgccgg tcttcaacga
      781 cgtctggaag aactggcgca aaactggaaa gggcggttgc caccgcgcag ctcactggcg
      841 atgatcgtgg ttaatcatac cgatatgcgt gttcgcggct gggtgggatc ggttgatctc
      901 aacgatgatt cacgctttgg tcatgttgat atggtcaatg cgatccgatc gccaggatcg
      961 gtgctcaaac cgtttgttta tggtctggcg ctggatgaag gcttgatcca cccggcatca
     1021 ctgctgcaag acgtcccccg gcgcaccggt gattatcgac caggtaactt tgatagcggt
     1081 tttcatggcc cgatcaacat gagcgaggcg ctggtgcgct cgctgaactt acctgctgtg
     1141 caggtgctgg aagcctatgg accgaaacgg tttgcggcaa agttacgcaa tgttggattg
     1201 ccgttatatt tgcccaacgg cgctgcgccg aatctttcac tcattctcgg cggcgctggt
     1261 gcaaaactgg aagatatggc ggcagcgtat accgcgtttg ctcgccacgg caaggcaggc
     1321 aaattgcgct tacagcctga cgatccgctg cttgaacggc ctttaatgtc gtcgggggcg
     1381 gcgtggatca ttcgccggat tatggctgat gaagcgcaac ccttgccgga tagtgccttg
     1441 ccgcgcgtcg ccccactggc atggaaaacg ggcaccagct atggctatcg tgacgcctgg
     1501 gcgattgggg ttaacgctcg ctatgtcatt gggatctgga ctggcagacc tgacggcacg
     1561 cccgttgttg gtcagtttgg ctttgccagt gccgtaccat tgttgaatca ggtcaataat
     1621 atcttactgt cgcgcagtgc gaatctgccg gaagacccgc gcccgaactc agtcactcgt
     1681 ggcgttatct gctggccggg cggacagtct ttgccggaag gtgacggtaa ctgtcgccgc
     1741 cgcctggcaa cctggctgct ggatggcagc cagccgccaa cgctgttact gtcagagcag
     1801 gaagggatta atggcattcg cttccccatc tggctggatg acaaagggca gcgcgtcgcc
     1861 gctgactgcc cgcaggcgaa gcaagaaatg gtaaatgtct ggccgctacc gctggaaccc
     1921 tggctgcccg catcagaacg tcgcgctgtg cgcttaccac cggcctcgac aatttgcccg
     1981 ccatacggtc acgatgccca gctccctctg cagctgacag gcgttcgcga tggcgcgatt
     2041 attaaacgtt taccgggcgc agcggaagcg actttgccgt tgcaatcaag tggaggggca
     2101 ggtgaacgct ggtggtttct gaatggcgaa ccgttaactg aacgcgggcg caacgtgacc
     2161 ctgcatttga cggataaagg tgattatcaa ttgctggtga tggatgatgt ggggcaaatc
     2221 gcgacagtga aatttgtcat gcaatagtct gttttgtccc tgattgttgc taaaactcat
     2281 tttattttaa aaaaaatgtt accttcatca atagtcaacg gccctgttgc tcattataat
     2341 ccgcgccatc tcgtacgctg gtacagacaa caacagaaca atttacagag gtaaaaatgg
     2401 ctattgaacg tactttttcc atcatcaaac cgaacgcggt agcaaaaaac gtcattggta
     2461 atatctttgc gcgctttgaa gctgcagggt tcaaaattgt cggcaccaaa atgctgcatc
     2521 tgaccgttga acaggcacgt ggcttttatg ctgaacacga tggaaaaccg ttctttgatg
     2581 gtctggttga attcatgacc tctggcccga tcgtggtttc cgtgctggaa ggtgaaaacg
     2641 ccgttcagcg tcaccgcgat ctgctgggcg cgaccaatcc ggcaaacgca ctggctggta
     2701 ctctgcgcgc tgattacgct gacagcctga ccgaaaacgg tacccacggt tctgattccg
     2761 tcgaatctgc cgctcgcgaa atcgcttatt tcttcggcga aggcgaagtg tgcccgcgca
     2821 cctgttaata atttcgtaaa tgccgcgtgc aaacgtggca tccgtgcgcc agaatttgta
     2881 caatgcagcg cccccggacg agcagccgct caccggggcg tttctttttt caaccctcca
     2941 ggggccataa cgtgtaataa cgaggccgga atagcatatg tctgaacaat tagtcacacc
     3001 tgaaaacgtc accacgaaag atggaaaaat caacctgctg gatctcaacc gtcagcagat
     3061 gcgggagttt tttaaagatt taggtgaaaa aaccttccgc gccgatcagg tgatgaagtg
     3121 gatgtatcac tattgctgcg acaactttga tgagatgacc gacatcaaca aagtgttgcg
     3181 cggcaaactg aaagaggtgg cggaaatccg cgcaccggaa gtggttgaag aacagcgttc
     3241 atccgacggc accattaaat gggcgatcgc cgttggcgat cagcgcgtcg aaacggtgta
     3301 tatcccggaa gacgaccgtg ccacgctctg cgtctcttcg caggtggggt gtgcgctgga
     3361 gtgtaaattc tgttccaccg cccagcaggg ctttaaccgc aacctgcggg tatcggaaat
     3421 tattggccag gtgtggcgtg cggcgaaaat cgtcggcgca gcaaaagtca ccggtcagcg
     3481 cccaatcact aacgtcgtga tgatgggcat gggcgagccg ctgctcaacc tgaacaacgt
     3541 cgttccggcg atggaaatca tgcttgatga tttcggtttt ggcctgtcta aacgtcgcgt
     3601 gacgctttcc acttccggtg tagttccggc gctggataaa ctgggcgata tgatcgacgt
     3661 tgcactggca atttccctgc acgcgccgaa cgacgaaatt cgtgacgaaa ttgtgccgat
     3721 caacaaaaag tacaatatcg aaacgttcct tgctgcggtg cgccgttatc tggagaaatc
     3781 caacgccaat cagggccgag tcactattga atacgtgatg cttgaccacg ttaacgacgg
     3841 cactgaacac gcgcaccaac tggcggaact gctgaaagat acgccgtgta agatcaacct
     3901 gatcccgtgg aacccgttcc cggacgcgcc gtatggacgc agttcgaaca gccgtatcga
     3961 ccgtttctca aaagtgctga tgagctacgg tttcaccact attgtgcgta aaacgcgtgg
     4021 tgatgatatc gatgctgcct gtggtcagtt ggcgggcgat gttatcgacc gtacgaaacg
     4081 tactctgcgt aaacggatgc agggtgaagc catcgatatt aaagcggtct gatagccatt
     4141 acgccacggt tactttatgt accgtggcgt aatcttttat gaatgaagta ttaagttgca
     4201 attgtctggt caatattaac ggtgcgtttt tgctgacttt aaggcagtat gtaacgatgc
     4261 aacagtaagt tagcctcagt gaatgtgggc tttgtcacga gcacacagac ggtcttatac
     4321 tgtatgataa cggttaactt aacggatgtt tcgcggtgtg ggtgagcatg atggttcacc
     4381 ggcatctcaa ttctcattta aacgtacctg cagcgaatga atactgaagc cacgcacgac
     4441 caaaatgaag cacttactac cggcgctcgc ctgcgtaatg ctcgcgaaca actaggactt
     4501 agtcagcagg ccgttgccga gcgactttgc ctgaaggttt ccacggtacg cgacattgaa
     4561 gaagataagg cacccgccga tcttgcttca acattcctgc gcggatatat ccgctcttat
     4621 gcgcgtctgg tacatattcc agaagaagaa ctgctgccag ggctggaaaa gcaggctcca
     4681 cttcgggctg caaaagttgc gccaatgcag agtttttccc tcggtaaacg ccgcaaaaaa
     4741 cgcgacggct ggctaatgac cttcacctgg ctggtgttgt ttgtggttat cggcctgagc
     4801 ggtgtctggt ggtggcaaga ccacaaagct cagcaggaag agatcaccac tatggccgat
     4861 caatcttcgg cggaactgag cagtaatagc gagcaggggc agagtgttcc gttaaatacg
     4921 tcgacaacta cagacccggc tacaacgtcc acgccgccag cgtctgtgga tactaccgca
     4981 accaacacgc aaacacctgc cgtaactgcg ccagcaccag ctgttgatcc gcaacagaat
     5041 gcggttgttt cgccttcgca ggcaaatgtt gataccgccg cgaccccggt accaacggca
     5101 gcaacaacgc cagatggtgc tgcgccgttg ccaaccgatc aggctggcgt gaccacgccg
     5161 gcggctgatc cgaatgcgct ggtgatgaac tttactgccg attgctggct ggaggtcact
     5221 gatgctaccg gtaaaaaatt gtttagcggg atgcagcgta aagacggtaa tttgaattta
     5281 accggccagg caccgtataa actgaaaatt ggtgcgccag ccgcagtaca gatccagtat
     5341 caagggaaac ctgtcgatct gagtcgtttt atcagaacta accaggttgc gcgtctgacc
     5401 ctcaatgccg aacaatcacc ggcgcagtaa cagacgggta acgcgggaga tttttcatgc
     5461 ataaccaggc tccaattcaa cgtagaaaat caacacgtat ttacgttggg aatgtgccga
     5521 ttggcgatgg tgctcccatc gccgtacagt ccatgaccaa tacgcgtacg acagatgtcg
     5581 aagcaacggt caatcaaatc aaggcgctgg aacgcgttgg cgctgatatc gtccgtgtct
     5641 ccgtaccgac gatggacgcg gcagaagcgt tcaaactcat caaacagcgg gttaacgtgc
     5701 cgctggtggc tgacatccac ttcgactatc gcattgcgct gaaagtagcg gaatacggcg
     5761 tcgattgtct gcgtattaac cctggtaata tcggtaatga agagcgtatt cgcatggtgg
     5821 ttgactgtgc gcgcgataaa aacattccga tccgtattgg cgttaacgcc ggatcgctgg
     5881 aaaaagatct gcaagaaaag tatggcgaac cgacgccgca ggcgttgctg gaatccgcca
     5941 tgcgtcatgt tgatcatctc gatcgcctga acttcgatca gttcaaagtc agcgtgaaag
     6001 cgtctgacgt cttcctcgct gttgagtctt atcgtttgct ggcaaaacag atcgatcagc
     6061 cgctgcatct ggggatcacc gaagccggtg gtgcacgcag cggggcagta aaatccgcca
     6121 ttggtttagg tctgctgctg tcggaaggca tcggcgacac gctgcgtgta tcgctggcgg
     6181 ccgatccggt cgaagagatc aaagtcggtt tcgatatttt gaaatcgctg cgtatccgtt
     6241 cgcgagggat taacttcatc gcctgcccga cctgttcgcg tcaggaattt gatgttatcg
     6301 gtacggttaa cgcgctggag caacgcctgg aagatatcat cactccgatg gacgtttcga
     6361 ttatcggctg cgtggtgaat ggcccaggtg aggcgctggt ttctacactc ggcgtcaccg
     6421 gcggcaacaa gaaaagcggc ctctatgaag atggcgtgcg caaagaccgt ctggacaaca
     6481 acgatatgat cgaccagctg gaagcacgca ttcgtgcgaa agccagtcag ctggacgaag
     6541 cgcgtcgaat tgacgttcag caggttggaa aaataataac gtgatgggaa gcgcctcgct
     6601 tcccgtgtat gattgaaccc gcatggctcc cgaaacattg agggaagcgt tgagggttca
     6661 tttttatatt cagaaagaga ataaacgtgg caaaaaacat tcaagccatt cgcggcatga
     6721 acgattacct gcctggcgaa acggccatct ggcagcgcat tgaaggcaca ctgaaaaacg
     6781 tgctcggcag ctacggttac agtgaaatcc gcttgccgat tgtagagcag accccgctat
     6841 tcaaacgtgc gattggtgaa gtcaccgacg tggttgaaaa agagatgtac acctttgagg
     6901 atcgcaatgg cgacagcctg actctgcgcc ctgaagggac ggcgggctgt gtacgcgccg
     6961 gcatcgagca tggtcttctg tacaatcagg aacagcgtct gtggtatatc gggccgatgt
     7021 tccgtcacga gcgtccgcag aaagggcgtt atcgtcagtt ccatcagttg ggctgcgaag
     7081 ttttcggtct gcaaggtccg gatatcgacg ctgaactgat tatgctcact gcccgctggt
     7141 ggcgtgcgct gggtatcttc gaacacgtaa ctcttgagct gaattctatc ggttcgctgg
     7201 aagcacgcgc caattaccgc gatgcgctgg tggcattcct tgagcagcat aaagaaaagc
     7261 tggacgaaga ctgcaaacgc cgcatgtaca ctaacccact gcgcgtgctg gattcaaaaa
     7321 atccggaagt gcaggcgctt ctcaacgacg ctccggcatt aggtgattat ctggacgagg
     7381 aatctcgtga gcattttgcc ggtctgtgca aactgctgga gagcgcgggg atcgcttaca
     7441 ccgtaaacca gcgtctggtg cgtggtctgg attactataa ccgtaccgtt ttcgagtggg
     7501 tgactaacag tctcggctcc cagggcaccg tgtgtgcagg cggtcgttat gacggtcttg
     7561 tggaacaact gggcggtcgt gcaacaccgg ctgtcggttt tgcgatgggc ctcgaacgtc
     7621 ttgtattgtt agtacaggcc gttaatccgg aatttaaagc cgatcctgtt gtcgatatat
     7681 acctggtggc ttcaggtgct gatacacaat ctgcggctat ggcattagct gagcgtctgc
     7741 gtgatgaatt actgggcgtg aaattgatga ccaaccacgg cggcggcaac tttaagaaac
     7801 agtttgcccg tgctgataaa tggggtgccc gcgttgctgt ggtgctgggt gagtctgaag
     7861 tggctaacgg cacagcagta gtgaaggatt tgcgctctgg tgagcaaacg gcagttgcgc
     7921 aggatagcgt agccgcgcat ttgcgcacgt tactgggtta aggaaggaga aggacagcgt
     7981 ggaaatttac gagaatgaaa acgaccaggt agaagcggtt aaacgctttt ttgctgaaaa
     8041 tggcaaagca ctggctgttg gggggatttt gggcgttggc gcactgattg gctggcgcta
     8101 ctggaacagc catcaggttg attctgcacg ctccgcttct cttgcctatc aaaatgcggt
     8161 taccgcagtg agcgaaggca aaccggatag catcccggcg gcggaaaaat ttgctgctga
     8221 aaataaaaat acttatggtg cgctggcttc tttggaactt gcgcagcaat ttgttgacaa
     8281 aaatgaacta gagaaagctg ccgcccagtt acaacagggg ctggcagaca cgagcgatga
     8341 aaatctcaaa gccgtgataa atctgcgtct tgctcgcgtt caggtacagc tcaagcaggc
     8401 tgatgccgcg ctgaaaaccc ttgataccat caaaggtgaa gggtgggctg ccattgttgc
     8461 cgacttgcgt ggtgaagcat tgctgagcaa aggtgataag caaggtgcgc gtagtgcatg
     8521 ggaagcaggc gtgaaaagcg atgttactcc ggcactgagc gaaatgatgc agatgaaaat
     8581 taataatttg tccatctgag agggacccga tgcaattgcg taaattactg ctgccaggac
     8641 tgctttccgt taccctttta agcggctgtt cgctgtttaa cagcgaagaa gatgtggtaa
     8701 agatgtcccc attgccaacc gttgaaaacc agtttacgcc gaccacggcg tggagcactt
     8761 ccgttggtag cggcattggc aacttctatt ccaatcttca tccggcactg gcggacaacg
     8821 ttgtctatgc agcggaccgc gctggtttgg taaaagcgct gaatgcggat gatggcaaag
     8881 aaatctggtc tgtcagcctg gccgagaaag atggctggtt ctctaaagag cctgcattac
     8941 tttctggcgg tgtgaccgtg tctggtgggc atgtctacat tggcagcgaa aaggcgcagg
     9001 tttacgcgct gaataccagc gatggtactg tggcatggca aactaaagtc gcgggtgaag
     9061 cactttcgcg cccggtggtc agcgacggtc tggtgttaat ccacaccagt aacggtcagt
     9121 tacaagcgct gaacgaagct gacggcgctg tcaaatggac agttaacctc gatatgcctt
     9181 cgctctcttt gcgtggcgag tctgcgccgg caacggcttt tggtgcggcc gtcgtgggtg
     9241 gcgataatgg tcgcgtcagc gcagtgctga tggaacaggg ccagatgatt tggcagcagc
     9301 gtatttccca ggcgaccggt tctaccgaaa ttgaccgtct gagcgatgtt gacacgactc
     9361 ccgtcgttgt taacggcgtt gttttcgcgc tggcctataa tggtaacctg acggcgcttg
     9421 atctgcgcag tggtcagatt atgtggaaac gcgaactggg ttcggtgaat gatttcatcg
     9481 tcgatggcaa tcgcatctat ctggtcgatc aaaatgaccg ggtgatggcg ttgaccattg
     9541 atggcggcgt tacgctgtgg gcacaaagcg atctgctgca tcgcctgctg acttctccgg
     9601 tgctgtataa tggcaacctg gtggtcggtg acagtgaagg ttatctgcac tggattaacg
     9661 tcgaagatgg tcgtttcgtt gcccagcaaa aagttgatag ttccggtttc cagactgaac
     9721 cggttgccgc tgacggcaaa ctgctgatcc aggcaaaaga cggaaccgtg tactctatta
     9781 cacgttaatc gtctctgtcg ttcactttga aaacggctcc tggacagggg ccgttttcct
     9841 gtttttaaca acgacgcgaa tatagcgtgc gttgtctgat gatttataaa aatgaggctt
     9901 taaacatggt acctgtggtc gcgcttgtcg ggcgccctaa cgtaggaaaa tccacgttat
     9961 ttaaccgtct aactcgcacc cgagatgcgc tggttgcgga tttcccgggt ctgactcgtg
    10021 accgtaagta cggtcgtgcg gaaattgaag gccgtgagtt tatctgtatt gataccggcg
    10081 ggattgatgg cacagaagac ggtgtagaaa cccgcatggc ggaacagtcg ctgctggcga
    10141 ttgaagaagc ggacgtcgta ctgtttatgg tggatgcgcg cgcgggcctg atgccggcag
    10201 atgaagcgat tgccaaacat ctgcgctccc gtgaaaaacc gaccttcctg gtggtaaaca
    10261 aaactgacgg tctggatccc gatcaggcag tggttgattt ctactcgctt ggtttaggtg
    10321 aaatctaccc gatcgccgcg tctcacggtc gtggcgtatt aagtctgctg gagcatgtgc
    10381 tgctgccgtg gatggaagat ctcgcaccgc aagaggaagt cgacgaagac gctgaatact
    10441 gggcgcaatt tgaagcggaa gagaacggcg aagaagaaga ggaagacgac ttcgacccgc
    10501 aaagtctgcc gatcaaactg gcgattgtgg gtcgtccgaa cgtaggtaag tctacactca
    10561 ctaaccgtat tcttggagaa gagcgcgttg ttgtttacga catgcctggc acgacgcgtg
    10621 acagcatcta catcccaatg gaacgcgatg gacgtgagta tgtgctcatt gacaccgctg
    10681 gcgtacgtaa acgcggcaaa atcaccgatg ctgtagagaa attctccgta atcaaaacgt
    10741 tgcaggccat tgaagacgcc aacgtggtga tgttagtgat tgatgcgcgc gaaggtattt
    10801 ccgatcagga tctctcgctg ctgggcttta ttctcaatag tgggcgctca cttgtcattg
    10861 tggtgaataa gtgggatggc ctgagccagg aagtgaaaga gcaggtgaaa gaaacgctgg
    10921 acttccgtct gggctttatc gattttgctc gtgtgcactt tatctctgcc ttgcacggca
    10981 gtggtgttgg taacttgttt gaatcagtac gtgaagcgta tgacagctcc acccgtcgtg
    11041 tggggacctc tatgctgacg cgcatcatga cgatggctgt tgaagatcac caaccgccgc
    11101 tggtacgcgg tcgtcgtgtg aagctgaaat atgcccacgc tggtggttat aacccgccga
    11161 ttgtggtgat tcacggtaat caggtgaaag acctgcctga ttcctacaag cgctacttga
    11221 tgaactactt ccgcaaatcg ctggacgtaa tgggatcgcc gattcgtatt cagttcaaag
    11281 aaggggaaaa cccgtatgcg aacaagcgta acaccctgac gccaacccag atgcgtaaac
    11341 gtaagcgtct gatgaagcac atcaagaaaa ataaataatt atccgtaagt attttatctg
    11401 ccattccctc tatgaatgta gagggaatgg ctaccggagg aattcatatg gaactgcatt
    11461 gtccaaaatg tcagcatgtg cttgatcagg ataacggcca tgcgcgttgc ccatcctgcg
    11521 gggaatttat tgaaatgaaa gctctttgtc cagactgcca tcaaccgttg caggtgttaa
    11581 aagcctgcgg tgcagtagat tacttctgtc agcacgggca tggcttgata tcgaaaaaac
    11641 gcgttgaatt cgtgctggct taatgaacct ttttacgcga tttttttacc ggctggatgt
    11701 tttttacttc actttctatc cagccgtctt ccagacgtgt ggttagcatt tcacccgttt
    11761 taacttgctt cacttttttc agtacattgc cgtcagtagc agtagtaacg ctgtatccac
    11821 gcgccagcgt tgacagtggg cttacggctt cgaggtgcgt tactgcatta ccgaaacgtt
    11881 cacgcgtggc gctaagctgt gcgcgcagga tttctgctaa acgatattcc agttgctgaa
    11941 tgcgcgtttg cgtgcgatga atcttcggct gtggattttg ctgattcagc cgctgtgtta
    12001 atcgctgctg ctgttgcccg gcacgcttaa gctgattttc cagcgcaaag ctcatccgtt
    12061 tttgcaggcg ttcaagcatg gtttgctggc gtgccagccg gagctgcgga tgctgctgct
    12121 gcaagcgatg atgaatctgc gtaaagcgac gtgtacggtt ggcgagataa taatccatcg
    12181 ccatctccag ccgttgacgg gccgattgca cctggcgcag taactcttgc tgattacggc
    12241 tcactacttc agcggcggca gacggcgttg gcgcacgcag atcggcaaca aaatcggcaa
    12301 tggtcacatc cgtctcatgc ccgacggcgc tgacgaccgg aatgcggctg gcaaaaatcg
    12361 cccgcgctac gcgttcgtcg ttaaaactcc ataaatcttc cagcgaaccg ccgccgcgcc
    12421 caacgatcaa cacgtcgcac tcattgcgct gattcgccag ttcaatggcg cgaacaattt
    12481 gccccggcgc gtcatcgccc tgaacggagg tggggtagat gatcaccggt agagaaggat
    12541 cgcgacgttt taacacatgc aaaatatcat gtagcgcagc accggttttt gaggtgatca
    12601 caccaacgca atgcgcaggg gagggaagtg gttttttgta ttgctgatcg aacaaacctt
    12661 cagcctgcaa cttcgctttg agctgttcgt acttctgttg cagcagccct tcaccggccg
    12721 gctgcatact ctcaacgatt atctggtagt cgccgcgcgg ctcgtagagc gtaatattgg
    12781 cgcgaactaa aacttgttgc ccatgctgcg gacggaaggt gacccggcgg ttgctgttgc
    12841 ggaacatcgc gcagcgtacc tgggcggtgt cgtctttgag tgtaaagtac cagtgaccgg
    12901 aagccggttg tgtgaaatta gagatttcgc cgctgatcca aacctgtccc atctcatgct
    12961 caagcagcag acgaaccgtt tgattcaggc gactaacggt aaaaattgca ggggattgag
    13021 aaggtaacat gtgagcgaga tcaaattcta aatcagcagg ttattcagtc gatagtaacc
    13081 cgcccttcgg ggatagcaag cattttttgc aaaaaggggt agatgcaatc ggttacgctc
    13141 tgtataatgc cgcggcaata tttattaacc actctggtcg agatattgcc catgctacgt
    13201 atcgctaaag aagctctgac gtttgacgac gttctcctcg ttcctgctca ttctaccgtt
    13261 ctgccgaata ctgctgacct cagcacccag ctgacgaaaa ctattcgtct gaatatccct
    13321 atgctttccg cagcaatgga taccgtaacg gaagcgcgcc tggctattgc tctggctcag
    13381 gaaggcggta tcggctttat ccacaaaaac atgtccattg aacgccaggc agaagaagtt
    13441 cgccgtgtga aaaaacacga atctggtgtg gtgactgatc cgcagactgt tctgccaacc
    13501 acgacgctgc gcgaagtgaa agaactgacc gagcgtaacg gttttgcggg ctatccggtc
    13561 gttaccgaag aaaacgaact ggtgggtatt atcaccggtc gtgacgtgcg ttttgttacc
    13621 gacctgaacc agccggttag cgtttacatg acgccgaaag agcgtctggt caccgtgcgt
    13681 gaaggtgaag cccgtgaagt ggtgctggca aaaatgcacg aaaaacgcgt tgaaaaagcg
    13741 ctggtggttg atgacgaatt ccacctgatc ggcatgatca ccgtgaaaga cttccagaaa
    13801 gcggaacgta aaccgaacgc ctgtaaagac gagcaaggcc gtctgcgtgt tggtgctgca
    13861 gttggcgcag gtgcgggtaa cgaagagcgt gttgacgcgc tggttgccgc tggcgttgac
    13921 gttctgctga tcgactcctc ccacggtcac tcagaaggcg ttttgcagcg tatccgtgaa
    13981 acccgtgcta aatatccgga tctgcaaatc atcggcggca acgtggcaac agctgcaggt
    14041 gcacgcgctt tggcagaagc tggttgcagc gcggttaaag tcggtatcgg ccctggttct
    14101 atctgtacga ctcgtatcgt tactggcgta ggtgttccgc agatcactgc cgttgctgac
    14161 gcagttgaag ccctggaagg caccggtatt ccggttatcg cagatggtgg tattcgcttc
    14221 tccggcgaca tcgccaaagc tatcgccgct ggcgcaagcg cggtgatggt aggttccatg
    14281 ctggcgggta ctgaagaatc tccgggtgaa atcgaactct accagggccg ttcttataaa
    14341 tcttaccgtg gtatgggttc cctgggcgcg atgtccaaag gttcctctga ccgttatttc
    14401 cagagcgata acgctgccga caaactggtg ccggaaggta tcgaaggtcg cgtagcttat
    14461 aaaggtcgcc tgaaagagat cattcaccag cagatgggcg gcctgcgctc ctgtatgggt
    14521 ctgaccggct gtggtactat cgacgaactg cgtactaaag cggagtttgt acgtatcagc
    14581 ggtgcgggca ttcaggaaag ccacgttcac gacgtgacca ttactaaaga gtccccgaac
    14641 taccgtctgg gctcctgatt ctcttcgccc gatttcatgt cgggcgattt atattatctg
    14701 tttcacttgc ctcggaataa gcgtcaatga cggaaaacat tcataagcat cgcatcctca
    14761 ttctggactt cggttctcag tacactcaac tggttgcgcg ccgcgtgcgt gagctgggtg
    14821 tttactgcga actgtgggcg tgggatgtga cagaagcaca aattcgtgac ttcaatccaa
    14881 gcggaattat tctttccggc ggcccggaaa gtactactga agaaaacagt ccgcgtgcgc
    14941 cgcagtatgt ctttgaagca ggcgtaccga tattcggcgt ttgctatggc atgcagacca
    15001 tggcaatgca gttgggcggt cacgttgaag cctctaacga acgtgaattt ggctacgcgc
    15061 aggtagaagt cgtaaacgac agcgcactgg ttcgcggtat cgaagatgcg ctgaccgcag
    15121 acggtaaacc gctgctcgat gtctggatga gccacggcga taaagttacc gctatcccgt
    15181 ccgacttcgt caccgtagcc agcaccgaaa gctgcccgtt tgccattatg gctaacgaag
    15241 aaaaacgctt ctatggcgta cagttccacc cggaagtgac tcacacccgc cagggtatgc
    15301 gcatgctgga gcgttttgtg cgtgatatct gccagtgtga agccctgtgg acgccagcga
    15361 aaattatcga cgatgctgta gctcgcatcc gcgagcaggt aggcgacgat aaagtcatcc
    15421 tcggcctctc tggtggtgtg gattcctccg taaccgcaat gctgctgcac cgtgctatcg
    15481 gtaaaaacct gacttgcgta ttcgtcgaca acggcctgct gcgtctcaac gaagcagagc
    15541 aggttctgga tatgtttggc gatcactttg gtcttaacat tgttcacgta ccggcagaag
    15601 atcgcttcct gtcagcgctg gctggcgaaa acgatccgga agcaaaacgt aaaatcatcg
    15661 ggcgcgtttt cgttgaagta ttcgatgaag aagcgctgaa actggaagac gtgaagtggc
    15721 tggcgcaggg caccatctac cctgacgtta tcgaatctgc ggcttctgca accggtaaag
    15781 cacacgtcat caaatctcac cacaacgtgg gcggcctgcc gaaagagatg aagatgggcc
    15841 tggttgaacc gctgaaagag ctgttcaaag acgaagtgcg taagattggt ctggagctgg
    15901 gcctgccgta cgacatgctg taccgtcatc cgttcccggg accaggcctt ggcgttcgtg
    15961 ttctgggtga agtgaagaaa gagtactgtg acctgctgcg ccgtgctgac gccatcttca
    16021 ttgaagaact gcgtaaagcg gacctgtacg acaaagtcag ccaggcgttc accgtcttcc
    16081 tgccagtacg ttccgttggc gtaatgggcg atggtcgtaa gtatgactgg gttgtctctc
    16141 tgcgtgctgt cgaaaccatc gactttatga ccgcacactg ggcgcatctg ccgtacgatt
    16201 tcctcggtcg cgtttccaac cgcattatca atgaagtgaa cggtatttcc cgcgtggtgt
    16261 atgacatcag cggcaagccg ccagctacca ttgagtggga atgattttga tccctgcact
    16321 atgaatgaac aaaaccctcc gttactacag agggtttttt atcttcaaga atcataggct
    16381 tgaagttact aacatcgatt aattaaacca gctgtccgat ttgttctctt ctgctttgcc
    16441 cacgcttttc atcagatcgc gaccgccttc agtcatattt ctgtttgcgt cagcttcaga
    16501 ttgcaccaca tcggtttgcg cagctttgtg cttcagttcc tgatcgataa attcgttttc
    16561 gcgcttaacg cgggcttctt ctttcgccag cgccagtttt tgtttctgaa tctctaagct
    16621 gcgtagctca tcttcataac tttgatcgcg ttttttgtcc gcagtggctt cggcgtccag
    16681 tttatcctga cgagctttct tatttgccgc tgccgttgcc gctcttttgt tcgcggcggc
    16741 ctgggcattt gcgcgacgtt gcttctcttg ctggatttcc ctgttgcgct ccgcgaccca
    16801 ttcgtcatgc tgcctttgct cttcattttt accttgctgt tccgcttctg cgacagcaga
    16861 gagttgatcc tgcaatgatg aggctatagc cggatagctt aaggaggcta agatggcgca
    16921 aagaaaaact ttcttcatga ctcctcctga ttattagctc ttttcaggac atttggtatt
    16981 tggctgaata cgcgtttcgt tatacgttgt ggtaataaca acggctaaac ctgtcgtaaa
    17041 ctggcactct ttaccctcct gggtggaggt atacactttg gtgccttcct tatatgttaa
    17101 agaaacacct tccactaagg ttttatcatt caccatagaa cccgctgccg cgcctacagc
    17161 tccgccgcca actgcccctg ccgtcgttcc ggaattgctg ccagaaccga cgttgtggcc
    17221 gataacaccg ccagcgactg cgccaataag cgcgccgaag gcttgtgcgt tccgtttatt
    17281 ttgggagttg tctacggcaa cttttgcggg aagaatggaa ataatattaa cggttttagt
    17341 ttcttgtttg gtattcagtt gatcggtttg ataaacatcg gcggcatgat cgtcagcatt
    17401 tgactggcat cctgccagag tgaatgacgc taacattgcc acaggcagaa gacatttttt
    17461 aaatttcatc actattcctt gttatacaca ttacgaaaaa taagacattt aagatcatca
    17521 aaagaggaaa gtaaatgtat tgcgtataaa gggatagatt aaatttatta aaattcacgc
    17581 agggaataat ttataaaaat atatcattac aacatatata ttaattttat atttatttcg
    17641 cagccaaaca ggataaacat cagtcaggac aaagacgtaa aatttaagat gaaagaatat
    17701 ctgccgcttt gataatccca aagagcaaaa gcatccgaca gcgaagtgcc gttatgttct
    17761 gtgaaagtgg agaaggtaac cgctaacgtt gtaccggtga ttcgacggag agtgtggatt
    17821 gctgagcttc ttttttgctc tgatggtgct gccaggcacc gacggacgag tagataaaac
    17881 ggccaaagaa gaagataaag ctgatgagca gtacgatacg ggtcatgcga ctgttaaatc
    17941 ggtgtcgttt tcgcatacta gttgcctgac tcacaaaagg ttccttgaag tatgtcccac
    18001 gctgtggacg gtacttacat taaggcacaa caggacaaaa tggtcaattc ttcgttatgt
    18061 aaaaaagcgt cagtggatac atattttaat gttatggaag ttaatttaat tatttacaat
    18121 gatgatgtaa taatgatgaa ttaacatgat aaacgtaata attcattaat cagggttatt
    18181 ttgtttgata tatatcaatt ggaactttca tatgacactt agaatcaatg ctctctatag
    18241 tgatgaataa tcatcattcg aagtcaggtg ggatgcctgt ctgaatacac ctccttcagg
    18301 atgtggggga ttctgctgag catctatgaa actgaatgca acttatataa aaatacgtga
    18361 taaatggtgg gggcttccgc tgttcctgcc ttctttaatc ttgcccattt tcgcccacat
    18421 taatactttc gcgcatattt cttccggtga ggtttttctc ttttatctgc ctttggcact
    18481 gatgatcagc atgatgatgt ttttcagctg ggcggcattg ccagggatca ccttagggat
    18541 ttttgtccgc aaatatgcag agctgggttt ttacgaaacg ctctcattaa cggctaattt
    18601 tattatcatt atcattctct gttggggcgg ttacagggtc tttactcccc ggcgtaacaa
    18661 cgtttcacat ggtgataccc gtttaatttc ccagcgtata ttctggcaga tagtgtttcc
    18721 tgcaacgctg tttctgatac ttttccagtt tgctgcattt gtaggattac tggcgagcag
    18781 agaaaatctg gtcggtgtca tgccttttaa cctcgggact ttaatcaatt atcaggcctt
    18841 actggtgggt aatctgatcg gtgtcccgct gtgctacttc atcattcggg tagtgcgaaa
    18901 tccattttat ttacgtagct attattcgca attaaaacag caggttgatg ccaaagtcac
    18961 caaaaaagag ttcgcgctct ggctactggc attaggtgct ttattgttgc tgttatgcat
    19021 gccgttaaat gaaaaaagca caatttttag caccaattat accttgtcat tattgctgcc
    19081 cctgatgatg tggggagcaa tgcgctatgg ctataagctg atttcgctgc tctgggcggt
    19141 cgtgttgatg atcagcatcc acagctatca aaattacatt cccatttatc ctggctatac
    19201 cacgcagctg accataacct cctccagtta tctggtattc tcttttattg tcaattatat
    19261 ggctgtactg gcaactcgtc agcgagcggt agtcagacgc attcagcggc ttgcgtatgt
    19321 ggacccggtg gttcatctgc caaatgttcg cgccctgaat cgcgcgttgc gtgatgcccc
    19381 ctggtctgcg ctttgttatt tacgcatccc tggcatggaa atgctggtta agaactatgg
    19441 catcatgctg cggattcaat
//
LOCUS       DBKELA010000083        19365 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.083, whole genome shotgun sequence.
ACCESSION   DBKELA010000083 DBKELA010000000
VERSION     DBKELA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.083"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            84..1580
                     /gene="ravA"
                     /locus_tag="ACUQR0_002868"
     CDS             84..1580
                     /gene="ravA"
                     /locus_tag="ACUQR0_002868"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HHV9109821.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1574..3025
                     /gene="viaA"
                     /locus_tag="ACUQR0_002869"
     CDS             1574..3025
                     /gene="viaA"
                     /locus_tag="ACUQR0_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HHV9109822.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3030..4022)
                     /gene="asnA"
                     /locus_tag="ACUQR0_002870"
     CDS             complement(3030..4022)
                     /gene="asnA"
                     /locus_tag="ACUQR0_002870"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HHV9109823.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4174..4632
                     /gene="asnC"
                     /locus_tag="ACUQR0_002871"
     CDS             4174..4632
                     /gene="asnC"
                     /locus_tag="ACUQR0_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HHV9109824.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4722..5165
                     /gene="mioC"
                     /locus_tag="ACUQR0_002872"
     CDS             4722..5165
                     /gene="mioC"
                     /locus_tag="ACUQR0_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HHV9109825.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5544..7433
                     /gene="mnmG"
                     /locus_tag="ACUQR0_002873"
     CDS             5544..7433
                     /gene="mnmG"
                     /locus_tag="ACUQR0_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HHV9109826.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7497..8120
                     /gene="rsmG"
                     /locus_tag="ACUQR0_002874"
     CDS             7497..8120
                     /gene="rsmG"
                     /locus_tag="ACUQR0_002874"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HHV9109827.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8737..9117
                     /gene="atpI"
                     /locus_tag="ACUQR0_002875"
     CDS             8737..9117
                     /gene="atpI"
                     /locus_tag="ACUQR0_002875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HHV9109828.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9126..9941
                     /gene="atpB"
                     /locus_tag="ACUQR0_002876"
     CDS             9126..9941
                     /gene="atpB"
                     /locus_tag="ACUQR0_002876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HHV9109829.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9988..10227
                     /gene="atpE"
                     /locus_tag="ACUQR0_002877"
     CDS             9988..10227
                     /gene="atpE"
                     /locus_tag="ACUQR0_002877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HHV9109830.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10289..10759
                     /gene="atpF"
                     /locus_tag="ACUQR0_002878"
     CDS             10289..10759
                     /gene="atpF"
                     /locus_tag="ACUQR0_002878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HHV9109831.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10774..11307
                     /gene="atpH"
                     /locus_tag="ACUQR0_002879"
     CDS             10774..11307
                     /gene="atpH"
                     /locus_tag="ACUQR0_002879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HHV9109832.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11320..12861
                     /gene="atpA"
                     /locus_tag="ACUQR0_002880"
     CDS             11320..12861
                     /gene="atpA"
                     /locus_tag="ACUQR0_002880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HHV9109833.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12912..13775
                     /gene="atpG"
                     /locus_tag="ACUQR0_002881"
     CDS             12912..13775
                     /gene="atpG"
                     /locus_tag="ACUQR0_002881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HHV9109834.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13802..15184
                     /gene="atpD"
                     /locus_tag="ACUQR0_002882"
     CDS             13802..15184
                     /gene="atpD"
                     /locus_tag="ACUQR0_002882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HHV9109835.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15205..15624
                     /locus_tag="ACUQR0_002883"
     CDS             15205..15624
                     /locus_tag="ACUQR0_002883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HHV9109836.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15975..17345
                     /gene="glmU"
                     /locus_tag="ACUQR0_002884"
     CDS             15975..17345
                     /gene="glmU"
                     /locus_tag="ACUQR0_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HHV9109837.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17507..19336
                     /gene="glmS"
                     /locus_tag="ACUQR0_002885"
     CDS             17507..19336
                     /gene="glmS"
                     /locus_tag="ACUQR0_002885"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HHV9109838.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4766 a         4852 c         5122 g         4625 t
ORIGIN      
        1 tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac gcagaacttt
       61 tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa agaatttccc
      121 gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc cgcttgtgtt
      181 tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt attgccaaaa
      241 gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt gaatatctga
      301 tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag gcgctaaaag
      361 atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa atcgtctttc
      421 tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc gccattaacg
      481 agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta ctggtggcgg
      541 cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac cgcatgctga
      601 ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg ctgaccagtc
      661 aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat gaagaatatg
      721 aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt gagctgattt
      781 ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg gatcgtcgct
      841 ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc agtgctgttg
      901 ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa agcctgaatt
      961 tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag caagggatgt
     1021 tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa caaagcgata
     1081 aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag cagtatcaac
     1141 ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg ttaaaactgc
     1201 atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag tggctgagta
     1261 aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa ctgaatctgg
     1321 aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc agtacgctgg
     1381 cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa ctggaagagc
     1441 ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa tgtctgttta
     1501 tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt gcacagattc
     1561 gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg tcagcgaaga
     1621 gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg cggtcttctt
     1681 tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct ggcgtgaggc
     1741 gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag aggtgatgtg
     1801 ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac cacagatcct
     1861 ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt tggttgatgc
     1921 taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc gtttaagtct
     1981 gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg aacaactgtt
     2041 aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc tcgcagataa
     2101 caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac gtggcgacta
     2161 tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga aacgcctggc
     2221 agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg cgcagatgga
     2281 aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg atggtctgca
     2341 acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag ggataacgga
     2401 actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct atcgcctgca
     2461 cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag attacgatga
     2521 acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg gcggctttaa
     2581 tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg ctgaaaaccg
     2641 gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt caggcccaca
     2701 aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca ccgatcttgc
     2761 cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg atgccgatgc
     2821 ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga gtaaagtgaa
     2881 agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg cacacggcaa
     2941 acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga tgcgaagccg
     3001 cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct ctcgcgaaca
     3061 gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag cagcagcata
     3121 gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc gcgcagcagc
     3181 gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag ctgatgcttc
     3241 agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc ttccagtacc
     3301 gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga cggggtgctc
     3361 cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa cttgccgcca
     3421 atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg ccctttggca
     3481 tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat ctgatccggc
     3541 aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt aattcccgcc
     3601 cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc gcccattacg
     3661 cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg gtcttcatcg
     3721 gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc gtgttgccct
     3781 aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc atcaggcaga
     3841 gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg cgtgccatcc
     3901 cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc ttccagttgg
     3961 cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta agcggttttc
     4021 attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg acacacatgc
     4081 aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga cggctaaaat
     4141 agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat cgacaatctg
     4201 gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc cgaactggcg
     4261 aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat gaagcaggcg
     4321 gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta tgacgtaggc
     4381 tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct ggcaaagctg
     4441 gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag catctttata
     4501 aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa gatccaaaca
     4561 attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat catgcgtacc
     4621 atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc ccaacgcgtt
     4681 cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat atcactctta
     4741 tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct gaaaagctgg
     4801 aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat ttatctgcct
     4861 cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg gacaaccttt
     4921 ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc cgctttggcg
     4981 caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat aaactcgagg
     5041 cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc aacattcttg
     5101 atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt aatttactca
     5161 aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc ttggatcaac
     5221 cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa cccctcattc
     5281 tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat cctttccagg
     5341 ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc taataagaga
     5401 tcacaataga acagatctct aaataaatag atcttctttt taatacccag gatcccaggt
     5461 ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc cattttcata
     5521 ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc atcatcattg
     5581 gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt caacagactc
     5641 tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg gcgatcggcg
     5701 gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg atggcgaaag
     5761 cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga ccggcggttc
     5821 gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt acggcgctgg
     5881 agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt gtcgaaaacg
     5941 atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa gctgtggtgc
     6001 tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat tacagcggtg
     6061 gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa ctgccgctgc
     6121 gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc atcgacttta
     6181 gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt atgggcaatg
     6241 cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag aaaacccatg
     6301 atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc gaaggtgtcg
     6361 gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac agaaatcagc
     6421 atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg aacggtatct
     6481 ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag gggatggaaa
     6541 acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat cctcgcgacc
     6601 tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt cagattaacg
     6661 gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt aacgctgccc
     6721 gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat ctcggcgtac
     6781 tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt acttcgcgcg
     6841 cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact gaaatcggtc
     6901 gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa cttgagaata
     6961 tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg gaagctgcag
     7021 ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt gaagatctgc
     7081 tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt gcccctgcgt
     7141 tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa ggttatatcg
     7201 cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg ctacccgcga
     7261 cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa cttaacgatc
     7321 acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct gcggccatct
     7381 ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca taacgcatta
     7441 aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta atcaccgtgc
     7501 tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat caccagaaaa
     7561 accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac aacctgactt
     7621 cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt gtggtggcac
     7681 cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg ccaggcattc
     7741 cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt ggtaaacgcg
     7801 tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa ccagtacaga
     7861 gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc cgcgcttttg
     7921 cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa ggccgtttct
     7981 acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa gaatatcagg
     8041 tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat ctggtggtga
     8101 ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac cggttagact
     8161 gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc acgttcactc
     8221 ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt cattagtgaa
     8281 aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa acattattaa
     8341 aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt atctgttttt
     8401 agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa tatgctgtgc
     8461 gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta cgcgttattt
     8521 acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc actggaaaaa
     8581 tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg ggcgcaccgt
     8641 ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt atacgacacg
     8701 cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct cgtgagtcga
     8761 aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag tggattgctg
     8821 ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct ggcagtcttt
     8881 ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac accagcgaaa
     8941 ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc gatgttggtg
     9001 ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat cgttacgtgg
     9061 gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa agggtaaaag
     9121 gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac ctgaataacc
     9181 ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca gccaccttct
     9241 ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg ttcctggttt
     9301 tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt cagaccgcga
     9361 ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat ggcaaaagca
     9421 agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg aatctgatgg
     9481 atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt ctgcctgcac
     9541 tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg ggcgtattta
     9601 tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg aaagagttga
     9661 cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt gaaggggtaa
     9721 gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg tatgccggtg
     9781 agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg atcctgaatg
     9841 tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc ttcatggttc
     9901 tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac actactacgt
     9961 tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct gctgtacatg
    10021 gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat cggcatcctc
    10081 gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct gctgcgtact
    10141 cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt aggtctgggt
    10201 ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga gcaatatcag
    10261 aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc tcggccaggc
    10321 catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc cattaatggc
    10381 agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag aacgagcaca
    10441 taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag cgaaagcgga
    10501 agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg acgaagcgaa
    10561 agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag aaattgaagc
    10621 cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg ctgttgctgg
    10681 cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca tcgtggataa
    10741 acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac ggtagctcgc
    10801 ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga acgctggcag
    10861 gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga gcttctctct
    10921 ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg tgagcaactg
    10981 gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct taacgcgctc
    11041 ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac cgctgaggta
    11101 gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc tgctgcgatg
    11161 gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc tgtaatggca
    11221 ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg tcgtcttgag
    11281 cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa ttccaccgaa
    11341 atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga agctcacaac
    11401 gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct ggccgattgt
    11461 atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact gaacctcgag
    11521 cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga aggcatgaaa
    11581 gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct gggccgtgtg
    11641 gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga cggcttctct
    11701 gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca gccggtacag
    11761 accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg tgaattgatc
    11821 atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat caaccagcgc
    11881 gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac catttctaac
    11941 gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt ggtagcaacc
    12001 gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc aatgggcgaa
    12061 tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc taaacaggct
    12121 gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga agcattcccg
    12181 ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt taacgccgaa
    12241 tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct gaccgcactg
    12301 ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa cgtaatctcc
    12361 attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat tcgtcctgcg
    12421 gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa gatcatgaaa
    12481 aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc agcgttctct
    12541 cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg tcagaaagtg
    12601 accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca gtctctggtt
    12661 ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat tggcagcttc
    12721 gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca agagatcaac
    12781 cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct cgattccttc
    12841 aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg caaggcattg
    12901 aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc gtccagaaca
    12961 cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt aaatcgcagg
    13021 atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt ggtcaccttg
    13081 cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt aaacgcgtgg
    13141 gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt aacctgttca
    13201 aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc gacctcgcaa
    13261 tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt gttgcccagg
    13321 tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta aaagtgatgt
    13381 tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac aaatttatta
    13441 acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca tcagatgatg
    13501 atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag gcgttgctgg
    13561 ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt gaaaacctgg
    13621 ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat ggcggcagcc
    13681 tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt actcaggaac
    13741 tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta gaggatttaa
    13801 gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg aattccctca
    13861 ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg agcgtctggt
    13921 gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa tgggttcctc
    13981 cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg aagtcccggt
    14041 aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg acatgaaagg
    14101 cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct acgaagagct
    14161 gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga tgtgtccgtt
    14221 cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa ccgtaaacat
    14281 gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt ttgcgggcgt
    14341 aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact ccaacgttat
    14401 cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc gtctgcgcgt
    14461 tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg acgttctgct
    14521 gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg cactgctggg
    14581 ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg gcgttctgca
    14641 ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg tatacgtacc
    14701 tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg acgcaaccgt
    14761 ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc cgctggactc
    14821 caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca ccgcgcgtgg
    14881 cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca tcctgggtat
    14941 ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga tccagcgctt
    15001 cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta aatacgtctc
    15061 cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg atcacctgcc
    15121 ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag ccaaaaaact
    15181 ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt cgtcagcgca
    15241 gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag cgaaggtgaa
    15301 ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg tatgattcgc
    15361 atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat tcttgaagtg
    15421 cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga tctcgacgaa
    15481 gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc tcacggcgac
    15541 gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct gcgcgttatc
    15601 gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca gtctggaaac
    15661 aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga tcctgctgat
    15721 ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa aacatccacc
    15781 tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg acgatggtcg
    15841 aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc tctgtccatt
    15901 tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt tctcaaatta
    15961 cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc gcaggcaaag
    16021 gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg aaagcgatgg
    16081 ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac ctggtgtacg
    16141 gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac tgggtgcttc
    16201 aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc tttgccgatg
    16261 atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa acactccagc
    16321 gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa ctggatgatc
    16381 cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt gttgagcaca
    16441 aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt ctgattgcca
    16501 acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct cagggcgaat
    16561 actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc gtcgccgttc
    16621 atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc tcccgtctgg
    16681 agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt atgctgcgcg
    16741 atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt gaaattgata
    16801 ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc ggcaccggtt
    16861 gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat accgttgtgg
    16921 aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg cgtcctggtg
    16981 ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa gcgcgtctgg
    17041 gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt ggcgataacg
    17101 ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa tttaagacca
    17161 ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg gtaacagtag
    17221 gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc gaaaatgcat
    17281 tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg gtaaagaaaa
    17341 agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa tctccctccc
    17401 acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc ccgaaaaaac
    17461 gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt gtggaattgt
    17521 tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac gtcgtctgga
    17581 ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc atatgactcg
    17641 cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac atcctctgca
    17701 tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt cagaagctaa
    17761 cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca tcgaaaacca
    17821 tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg aaaccgacac
    17881 cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc tgcgtgaggc
    17941 tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca tggactcccg
    18001 tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg gcctgggtat
    18061 gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc gtcgctttat
    18121 cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca tcttcgataa
    18181 aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg acgcgggcga
    18241 taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga acgcgataaa
    18301 aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc tgggaccgaa
    18361 cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg gtacttctta
    18421 taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc cgtgcgacgt
    18481 cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca gcctgatgat
    18541 caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc tgtcgaaaga
    18601 gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc tggtgcgcga
    18661 atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt ccactaaagc
    18721 attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc gcctgaaagg
    18781 tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc cgagccgtat
    18841 tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt tctctgacaa
    18901 acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg aaggcgcatt
    18961 gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg agctgaaaca
    19021 cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac cgaacaacga
    19081 actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg gtcaattgta
    19141 tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca tcatcgagat
    19201 gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc agctgctggc
    19261 ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc tggcaaaatc
    19321 ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DBKELA010000084        19260 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.084, whole genome shotgun sequence.
ACCESSION   DBKELA010000084 DBKELA010000000
VERSION     DBKELA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.084"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            31..330
                     /locus_tag="ACUQR0_002886"
     CDS             31..330
                     /locus_tag="ACUQR0_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HHV9109839.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            327..2075
                     /locus_tag="ACUQR0_002887"
     CDS             327..2075
                     /locus_tag="ACUQR0_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HHV9109840.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2364..2642
                     /locus_tag="ACUQR0_002888"
     CDS             2364..2642
                     /locus_tag="ACUQR0_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109841.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2639..2944
                     /locus_tag="ACUQR0_002889"
     CDS             2639..2944
                     /locus_tag="ACUQR0_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109842.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2954..3115
                     /locus_tag="ACUQR0_002890"
     CDS             2954..3115
                     /locus_tag="ACUQR0_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HHV9109843.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3103..3216
                     /locus_tag="ACUQR0_002891"
     CDS             3103..3216
                     /locus_tag="ACUQR0_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HHV9109844.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            3257..3481
                     /locus_tag="ACUQR0_002892"
     CDS             3257..3481
                     /locus_tag="ACUQR0_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109845.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3782..4939
                     /locus_tag="ACUQR0_002893"
                     /pseudo
     CDS             3782..4939
                     /locus_tag="ACUQR0_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4979..5551
                     /locus_tag="ACUQR0_002894"
     CDS             4979..5551
                     /locus_tag="ACUQR0_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HHV9109846.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5553..6764
                     /locus_tag="ACUQR0_002895"
     CDS             5553..6764
                     /locus_tag="ACUQR0_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHV9109847.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6761..7099
                     /locus_tag="ACUQR0_002896"
     CDS             6761..7099
                     /locus_tag="ACUQR0_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HHV9109848.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7096..7392
                     /locus_tag="ACUQR0_002897"
     CDS             7096..7392
                     /locus_tag="ACUQR0_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HHV9109849.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7392..7832
                     /locus_tag="ACUQR0_002898"
     CDS             7392..7832
                     /locus_tag="ACUQR0_002898"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HHV9109850.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7816..7998
                     /locus_tag="ACUQR0_002899"
     CDS             7816..7998
                     /locus_tag="ACUQR0_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109851.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8054..8344
                     /locus_tag="ACUQR0_002900"
     CDS             8054..8344
                     /locus_tag="ACUQR0_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HHV9109852.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8364..10025
                     /locus_tag="ACUQR0_002901"
     CDS             8364..10025
                     /locus_tag="ACUQR0_002901"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HHV9109853.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10039..10320
                     /locus_tag="ACUQR0_002902"
                     /pseudo
     CDS             10039..10320
                     /locus_tag="ACUQR0_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="ACUQR0_002903"
     CDS             complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="ACUQR0_002903"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HHV9109854.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="ACUQR0_002904"
     CDS             complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="ACUQR0_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HHV9109855.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13417..14787)
                     /gene="purB"
                     /locus_tag="ACUQR0_002905"
     CDS             complement(13417..14787)
                     /gene="purB"
                     /locus_tag="ACUQR0_002905"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HHV9109856.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="ACUQR0_002906"
     CDS             complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="ACUQR0_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HHV9109857.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="ACUQR0_002907"
                     /gene_synonym="trmU"
     CDS             complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="ACUQR0_002907"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HHV9109858.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="ACUQR0_002908"
     CDS             complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="ACUQR0_002908"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HHV9109859.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="ACUQR0_002909"
     CDS             complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="ACUQR0_002909"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HHV9109860.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17924..19174
                     /gene="icd"
                     /locus_tag="ACUQR0_002910"
     CDS             17924..19174
                     /gene="icd"
                     /locus_tag="ACUQR0_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HHV9109861.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5014 a         4727 c         5158 g         4361 t
ORIGIN      
        1 taaagaacaa cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc
       61 agtggcaaca ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc
      121 atggtaacga ttaaaggcgt ggcagaacgg cgcatcacct accgccgtga aggttacgaa
      181 tatgattgcg tgatgcctgt ttatcaattc cggcgtgatt tcacgctggt acaggcagcg
      241 ccccgcagta aaccaaccag cagggagaaa gcacgcgcca atattcagga aataaaaaag
      301 atgcttaacg tattcagggg taaaaaatga aactggcacc gaacgtaaaa aaacagccac
      361 gcggaataaa acacaaagac acagaggtaa ttatctttgc gggtagtgat gcctggtcgc
      421 acgcgaaaca atggcaggag caggatggcc ccgcatccgg cgataatgtg ccgcctgtgt
      481 ggcttgggcc aaatcagctt gccgaacttg atgcactgaa aattgttccg gatggaaaaa
      541 aacgcgtaag gctgtaccag gccggagaac tggatttggt ggagaccaaa aagattggtc
      601 agaagctggc ggcggcagat attcaggacg caaattttta ccccgaagga atgcacgtcc
      661 agaagtgtga aaactggcgg cgctatctga atgctgagcg tgaaaatatt gccgcagggc
      721 ttaccatgcc ggagcagaaa aatacgcaac tggcacaaat ggcagacagt gagcgcgcgc
      781 agctgcttgc cgagcgcttt gatggcgttt gtgtgcatca ggaaagtgaa atcgttcacg
      841 tatggcgcgg cggggtatgg tgtccggtca gcacaatgga acttagccgc gaaatggtgg
      901 cgatctattc agagcacagg gccactttca gcaagcgcgt aatcaataac gccgtggaag
      961 cgttaaaagt tattgccgaa cccatgggcg agccgtccgg cgatttgctg ccgttcgcca
     1021 atggtgcgct tgacctgaaa acgggggaat tttccccgca cacgccggag aactggatca
     1081 ccacgcacaa cggcattgag tacacgccac cagcaccagg ggagaacatc cgcgataacg
     1141 cgctaaactt tcataaatgg cttgagcacg cagccggaaa agaccagcgc aagatgatgc
     1201 gtatatgtgc cgcgctgtac atgattatgg cgaaccggta cgactggcag atgtttattg
     1261 aggccaccgg agacggcggg agcggtaaaa gtacattcac acacatagcc agccttctgg
     1321 cagggaaaca gaacacggta agcgctgaaa tgacatcgct tgatgatgct ggtggacgtg
     1381 cgcaggttgt cgggagtcgt cttatcgtcc tggctgacca gccgaaatat acaggcgaag
     1441 gaacgggcat caagaaaatc acgggcggtg accccgcgga aattaacccg aaatacgaaa
     1501 agcgtttcac ggctgtaatc agggcggtgg tgctggcgac caacaataac ccgatgatat
     1561 tcaccgaacg ggccggaggt gtggcacgtc gtcgcgtgat tttccgtttc gacaatatcg
     1621 ttagtgaggc cgaaaaagac agggagctac cggaaaagat tgcggctgaa atccctgtca
     1681 ttatccgccg attgctggcg aactttaccg actctgagaa agcacgggct ttactactgg
     1741 aacagcgtga cggtgatgaa gctctggcaa taaagcaaca gacggatccg gttattgagt
     1801 tttgccagtt cctgaatttt ctggaggaag cacgcggcct gatgatgggc ggcggtggcg
     1861 attcagtgaa gtacacgacc agaaacagcc tttaccgcgt ctatctggcg tttatggcgt
     1921 acgcaggcag gagcaaaccg ctaaacgtaa atgactttgg caaggctatg aagccagccg
     1981 cgaaagttta cggacatgaa tatattacgc ggaaagttaa gggagtaacg cagactaacg
     2041 caataacaac agacgagtgc gacgcgtttt tataattttt tgtaattgct gtctaccctg
     2101 tctacctgag taaagaaaaa tacatttaat tcagtgcatt aagttaggta gatagccttt
     2161 ttttactgtc tacccactat ctaccctctc tacctgattt tacctgaatc agacagggag
     2221 gtagacacgg ggtagatagt ggataaaagc actctacccc gctgaaagca gcgccattac
     2281 tggcatgagg gccactaagg tagataaggt agacaagggg aggcacaact caaaactttt
     2341 taaacgaggg ggtaaaaata aatatgcaca cttcagggaa atttaataaa tcattcaaaa
     2401 aacgcagaga cagaacagaa ccgaaatatc gcgcgttaga catgacagag cacgctttaa
     2461 aggtggcaat cagaacgata gaccgccacg cgggggaagg atacgcgaaa gcacatcccg
     2521 aactgataag cgcattcatg accacgacgg cggcaaattt tgccacgctg acagagcggg
     2581 agattgccga agcggaacag gtaacaacaa tcaacgttaa aaccggagag gtggaatcat
     2641 gagcgaacca aaatttggtg aaaaatttta caagcacaac ggacggatca cgattctgca
     2701 aataagcgca gctacaccag gctggtgggt tgaaacagac gaaggatcct cacccgtggc
     2761 atcatgggcg ctttgtgtgc ttagttaccc tgaccgcgat gtgtatcagg atattctgcc
     2821 tgtgatatcc actgacaaag gcatgaaacc cgtagacatc aaaaacatgg ggtttcagtg
     2881 cgtaatgctc actgaaaaaa tgatggaaga gatgaaaaag aacaatgccg gatcgcttca
     2941 ctgaggtggc aatatgacag cacaaattgc agcttacggg cggctggtgg gcgacccgct
     3001 ggtaaaacag agcagcaagg gcacaccgga gccgccaacg gagaactacg cagacctgag
     3061 aaatgcagta aatcgcactt acgcagacat ggggctgagc aaattagcag agtaggaata
     3121 tcagaaccac accacgcagc cggagcaatc cggctttttt tgcgtcattt gaaacggtat
     3181 ggagcactac tggcttttcg tcacggtccg gcatagttac tatctgaaac aaacagacac
     3241 aacagaggaa aacgcaatgc cgatgaaatt tgatgagata cttaaacagc gtgataaata
     3301 ccatgctgac aacatggaga cgatgaatat cactgattac cgcgcatttc tggaaacggg
     3361 ggcgctgatt gaaaaggatc atcatggttt tgttcgttgc gcgctatccg gtgaaatgct
     3421 ggcagtaaat cctgaacaga cagatgcatt gatagaattt ctgaaaggga tcagagactg
     3481 agcacccata cagcacatac agccggagca atccggcttt ttgttatttt ttgtaaatta
     3541 tttgttcgtg gttgttccac gttgttcact gagcggatcg gcatatttta cccgaactga
     3601 atcatgatta ttctcgcccg tggtgccagg acgctggggc cactttcccg cctgttaatg
     3661 tgctcgccaa tattcattac caggcgggaa aacgatcggt gcgattactg atttccttat
     3721 gaaaaacggt tgagtgtttg ccgcgtcctg gagttcctta cttaacccca ggactttttt
     3781 tatgcagaga ataatcgaat tacgccagca gaaaaccgcc attaaaaatc agatgcgcga
     3841 catgctggag aacgctgaaa aagaaaaccg cagtcttaac gatgctgagg gcacaaaatt
     3901 tgacgaatta cgcgctaaag ctgaatccct cgataaagac atttcccgcc ttgaagccat
     3961 tgcagacgaa gagcgcagca agccaggtaa aagcagccag accactgacc ccgcagaact
     4021 acgcaactac attctgaccg gtgaaacccg cgcattaagt acaggcgttc ccgctgatgg
     4081 tggttatacc gttatccctg aactgaacac cgaaatcatg cgaatgctgg cggatgaatc
     4141 caccatgcgt cgcatctgta ccgtgaagaa aatcagcagc aacgagttta agcagcttgt
     4201 ttccgctggc ggtgcgaccg ttaaccacgg tgaagagggt aaggcacgcg aacagaccag
     4261 caccccgcag attaacgagg tgagcattaa gctgtatccg gtctatgcgt acccgcgcac
     4321 cacacaggaa atcgtggatt tttccgatgt ggacatcctt tcatggctga cgggtgagat
     4381 tggcgacacc ttcacggaaa ccgaagaaag cgatctggtt gtgggcgacg gtgacaaaaa
     4441 agcaaaaggt tttttatccg taccccgtgc agagaagaac gacaaagagc gtgattttgg
     4501 tacgttgcag gtaattaaac cttccgaatc tctggcgtgg acatctgcgg acccgctgat
     4561 cgacctgaaa tttgcattac gtaaaaaata ccgcaaaaac gcggtctgag tggttaactc
     4621 cacgacggcg gcaaaacttc agaaggtgaa gaacgcgaac ggtgattata tctggcgcga
     4681 ccgtttacag acgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt
     4741 tatgcctgat aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca
     4801 cgaaacaggt gttcgcacca gaccggacaa cctgacagaa ccaggattca tcaaaatttt
     4861 cacgcaaaaa tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggcact
     4921 gccggaagac gacgattaac agcatacaga aggggctaaa aagccccttt agtgttttat
     4981 gggtgaaaaa attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcggagc
     5041 cggtacgctg acgggctacg ttgttcgctg ggataagctt tcagaactgt tatggggtga
     5101 gttttacgaa aaattccagc ggggggcgtt tactgaatgg cttgcggcgg gtaatgacgt
     5161 tcgcggcctg tatgagcatg accacagcat gttactgggg cgcacccgtt ccggcacgct
     5221 gaaactggaa gaggacgaaa cagggttacg ctttgaactg accccaccgg ataccagtac
     5281 agggcgcgac gttatcgaac tggttaagcg cggggacatc tcggggatga gctttggctt
     5341 tcgctcccgt aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt
     5401 ggcggaactg tacgaaatta ccgttacatc ggtaccggct taccccgatt ccggcgtgga
     5461 gctggcccgc cgttccctgt atgagcagca ccccgaaaaa atgccgcgtg cggataatcg
     5521 ccgctggtgg gcggatttag cgggggtgtg atatgtggcc tttcagacga aaaaaagagc
     5581 agcgcagcat gacgcttgat gaatttatgg cgctggccgg cacatcgaac acgggggcgg
     5641 gtgagtacgt atcatctggg acagcggaat cactccccgc cgtcatgaac gccgtcacgg
     5701 tcatctctga cgcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg
     5761 ggaaggaggc ccgcgaatgg cttgattctc atccggtcga tcacatcctc aacgagcgcc
     5821 cgaacgcgtg gcagacaccc taccagttta agcgaatgat gatccgctac tgcctgttaa
     5881 gtggtaatgc ttatgcggtt attcagtggg ggcgtgatgg ttttccggcg gctttacatc
     5941 cttacccgcc gcagtcggtg aacgtggagc agacaggtga tcataactgg cgctactgca
     6001 tcactgacgc ctacaccgga aacacccgca actatttacc gtgggaggtt ctccaccttc
     6061 gttactccac ggatgacggt tttatggggc gctcacctgt aaccatctgc cgcgaatcgc
     6121 tggggcttgg gctggcccaa caacgccacg gcgcgagcgt gatgcgtgat ggcatgatgg
     6181 cggcaggggt tatcacgtca ggcgaatggc tggacggcgt gaagggcaaa caggcattag
     6241 ccgcactgga acgctacaaa ggggccagaa acgccggaaa aacgcccatc cttgaagggg
     6301 ggatgagcta tcagcagctg ggcatgagta atcaggatgc cgaatggctg gcctcccgtc
     6361 gcttcaccat tgaagacatc gcccgaatgt tcaacgtctc gccgattttt ttgcaggaat
     6421 acagcaacag cacctacagc aacttcagcg aggcaagccg cgcatttctc accatgacga
     6481 tgcgcccgtg gctggcgaac tttgagcagc agataaaaaa cgccttgctg gtggcctcgc
     6541 ctgtacctgg tatccggtat caggtggagt ttgacagcgc ggacctgtta cgggccacac
     6601 ctggcgaacg ctttgccacc tatgagcgcg gcataaaatc cgacgttatg tgtccgaacg
     6661 aagcccgcga acgtgaaggg ctgtcaccgc gtgatggtgg tgatgagttc agccaggcat
     6721 ggaaacagga agtaaaaatc agcgagggag aaaaaccgga atgaacatag ggcgactgcg
     6781 tgacaggata acgattcaga ccctgaaaca gaccagggat ataaccggcg aaatactcga
     6841 aacgtgggag gacggtcaca cactctgggc aagcgtgaac atggtcagca gcaaggaggc
     6901 catttcatcg ggcgcagagc tggcgattgg taccgtaagg atctggatac ggtaccggaa
     6961 ggacatcaac gccaccagcc ggataaaggt cagtacgggg ccgctggcgg ggcgtgtact
     7021 gaatatcatc gggcagccgc tgccggatgc cgccaggaca cgccttgaaa ttctttgtcg
     7081 tgagggcgcg gaaaaatgac agaagaactt atcaccctgg aagaagtgaa actccattgc
     7141 cgcatcgatg gcgacgatga agaccagtta atcagcggat acattgccgc atcgcttgag
     7201 gcgtgccaga tacacatagg caggcgcttt gatgacgggc tggcgttcac gccagccata
     7261 aagattggct gcatgatgtt tatcgctcac ctgtatgaaa atcgccagat tgtcgcggat
     7321 aacgcaaaaa cacgcgtacc catgacgata ggcgcactct ggacggctta ccgtgatgtg
     7381 ggggggtact gatgccgtgg caaccattaa gacgatgcac agaaccaggc tgtaacaggc
     7441 gcgttaaatc cggcaagtgt gaggagcaca ggcgggcggc atggcgtgca gaggatgcca
     7501 gacgaggaca ccgccgcgcg cgtggatatt ccagacagtg ggacaaatac cgcgccatgt
     7561 atctgagtaa aaacccgtta tgcgtgcgat gccttgagaa ggggatatat acgcccgccg
     7621 tggtggtgga tcacattatc ccgattaatg gcggtgatga tgttctcttc tggcccgaat
     7681 ggaaccatca accattgtgc caggcgtgtc ataaccagaa aacgaaatgg cttgatccgg
     7741 caacaaaaag caagcgtgcc gcaggtggat ttcgtgaaga ggaagaacgg gctgctaacc
     7801 gcaataactg gatgtatgac gctgatgaat gagcgggaac aaaaccgcct tatccgtgga
     7861 cttataaggc agcgtgacgc atggaagaca caggaaacag ggcataaaga taaagcgtca
     7921 ggacgcgcag aacgcatcac agcgaagcga ttaaccgacc gtgaccgcga ggttatggaa
     7981 tgtttccgca atcgctgatg aggctgtatg acggggtggg gggcgttttc aggacaaacc
     8041 cgaccccgcc tacctggatg atatcgccgt gaagcagtgg cgggaaaaat cgcggcagct
     8101 tgcggaacag ggagacctga cccccgccga ctggagcaat ctggaactgt attgcgtcaa
     8161 ctactccatt taccggaaag ccgttgcaga ccttgcggcg cgcgggttca gcattgttaa
     8221 cagtcagggc ggcgagagca gaaaccccgc attaagcgca aaatccgacg ctgaaagagt
     8281 gatgataaaa atggcctcct tgctcggttt tgacccgata agccgccgta aaaatccacc
     8341 gtaaacagaa gaagaggacg agcttgaccg cctggaataa gtacgcagaa gacgtaaaaa
     8401 cgggcaaaat tccggcctgt aaacggttaa aacaggctgt taaacggtac ttttcggacc
     8461 ttaaaagccc cctttacacg ttcgatcgtg aggttgtgga gcggtttatt gccttttcca
     8521 gggtgtgccc gcacgtaaaa ggaccgatgc gtggcagacc cattgagctg gagccgtggc
     8581 agcagttcgc ctttgcgtgc atcctcggct ttaaggttaa ggccaccgga cggcgcaaat
     8641 acacgagcgc ctttatcgaa gtgccgcgca aaaatgcgaa atccacgacc gccgcgattc
     8701 tggctaactg gtttctgatt atggagaacg gtcagcagga tatctacacc gcagcggtga
     8761 gccgtgacca ggcgcggatc gtgtttgata atgcgcgtca gatgtgcctt ttatcccgac
     8821 cgttacgcag gcgggtgaat attcaggcgc ataaggtgat acacccgaaa agcaacagcc
     8881 tgttaaagcc gctggcagca aaagcggcaa ccatcgaggg gacaaacccc agtctttcca
     8941 tcgtggacga ataccaccta caccccgata acggcgttta ttccgcgctt gagctgggga
     9001 tgggcgcacg tcccgaaggg ctgttatttg ccattaccac atcgggcagt aacgtcgttt
     9061 cagcctgtaa acagcactat gattattgct gccagatcct ggacggcgaa gaggtcaacg
     9121 attcaatttt tgtactgatt tacgaactgg acgacgaaaa cgaggttgat gatccggcga
     9181 tgtggataaa ggcgaaccct aatattgatg tttccgtgga tcgtgaaaaa ctggcctcaa
     9241 ccatccagaa agcgcggggt attccgtcgc agtgggtgga gatgatgaca aagcgattta
     9301 atatctggtg tcaggggact accccgtgga tgggtaatgg tgcatgggct gagtgtgccg
     9361 gaacgttcac ggaggaagat ttacacggtc aggagtgtta cgcggggctg gacttatcat
     9421 caaccagcga tatttccagc gtatgctatg cctttccggt cggtaaaacc attatgctga
     9481 tttcccgtca ttatctgccg gagttccagc tacagaaccc cgccaataaa aaccgcgctg
     9541 tctatcgcca gtgggctaaa gcgggctgga tacgcacaac gccaggtgac tgcattgatt
     9601 acgaccggat cagagatgac atcatgcagg atgcggagaa atttaatatc aggctggtgg
     9661 gctttgatac gtggaacgcc acgcatctca ggacgcaatt acagggggct ggttttgagg
     9721 tggagccgtt cccgcaaacc tacctcagat tcagtccggc ggcgaaatcg ttcgaagttt
     9781 ttgttaaccg cagggtgatt gtgcatcgtg gcgatccggt gttgtcctgg tcgatgagta
     9841 acgtcgtgat gcagagtgac gcgaacgcca atatcaagcc gaacaagaaa aaatcaccga
     9901 acaagataga cccgagcgta gcggcgctga tggcgtttgg tacattccag gcagagcatg
     9961 aggattttgc ttttgatata agcgacagcc accgccagaa actggaagaa tttagcgggg
    10021 tgtaatgagg tcagcagcat gacagaagcc gaaatactgc gattaatccg ccgcgtctct
    10081 ggaatcagcc agtaggctga cgaacagacc acgcagccgg acagcgtgac agccgaaaat
    10141 tatgcgcgtg ttgttgctga ggtgatgcgc cgtgatggta tccagcttaa tgatgcggat
    10201 atgcgcgaca tacggatccg cgttctggaa atgctggcct acaatcgccg cgttgaactg
    10261 tatcgggaga aggagaaaat cacgtaccac tggaagaagc cggagcggtt acggcggtaa
    10321 ctcactgaga ttaaagcaaa gcgcaaaatt gcgctggcta catccctcac atctgaggac
    10381 tgttaaccag caggccggaa tatattcaga aattacgaaa ttttcgcagt ttaattactc
    10441 gtcagcttaa tcgctaaccc gctctgatat tttcggaaac ctcaatttga ggaagtcggc
    10501 gaggtaactg tttgatattc ccgatagcgc aaaaatgcgc ggtcgggcag aatgaccacc
    10561 acctcaatcg tgacggagag ttacaaccag tgacacaccg tgacaaaaag acggtttaac
    10621 cgttcattaa aaatcgacgt gattttgtgc tttccggatg acgtggccaa catcattttt
    10681 tagcgaaaaa ttctgattaa ggtcactggc tgaacggtca taatgaccac gcagaccatc
    10741 gaggcaacca tagcgatcat cgttacggtt ggagcttttc cgccattggt ggaaaaggtg
    10801 cacatctgga tcatcattac ggtgatcaag ggtatgtaaa acccaccagc ctgattaaag
    10861 gttaaccgga aaaaaaatcc ggtagccccg ttatggggat ccccatatcg acattaacgc
    10921 ccctcatgaa ttggtgcgct tccccctgga aagattaccc gcctgtatat ttcttgtgtc
    10981 tatttgttcc acgttgtttc atacagtgca ccgaacggtg tagttaatgg tgtagtcaat
    11041 ttgcgatttt tggcactttt tcaatgaaaa ttgtttgatc gaatcaatga cttgatgtag
    11101 tggtaaaagg acatatttat tcatctttcg gcgcagaatg ctggcgacca aaaatcacct
    11161 ccatccgcgc accgcccagc atgctctctc cggcgacgat tttaccctca tattgctcgg
    11221 tgatttcgcg ggctaccgcc agccctacac cttgcccagg gcgtaaagta tcaacccgtt
    11281 gaccacggtc gaaaatgacc tctcgcttgc ttaatggaat accggggcca tcatcctcga
    11341 ccacaatata gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat
    11401 atttacaggc attatccagc acgttgccca tcacctcgac aaaatcgttc tgctcaccga
    11461 caaagctgat ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt
    11521 tgttcagcgc tgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga
    11581 gcaatgtccc gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc
    11641 ggctgatttg ctccagcatt accggctcag catcactgac gctcatcttt tcactacgca
    11701 gagaacgcag cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg
    11761 tgagcgtcgt acggtatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt
    11821 ttcgtaccag actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt
    11881 cttccagttc gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg
    11941 cgacccacag cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc
    12001 tccagaccat ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta
    12061 attttggcat ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg
    12121 cgtcgtcatc atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca
    12181 gcaagaggct ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc
    12241 agtcaggctg gatcattttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc
    12301 cgttctcatc ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact
    12361 cgacatgcaa cttattgttt tcccactgcg caagggtata gaacagattg ctctcgccac
    12421 gtaacagccg aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc
    12481 cgtaggcaag cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca
    12541 gcgagagcgg gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acaggtagcc
    12601 ctggccgcga acggtggtaa tgacttcttg cggatattgt gcctgaattt ttttgcgcag
    12661 acgtcccatc agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg
    12721 tagcattaac gaatctttgc tgaccacttt gccattattg cgtatcaacg tttccataat
    12781 ggtgtattcg aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga
    12841 gagatcaacc tgaaacgggg ggagcgaaat gacctgtgaa gccagaccgc tattacgccg
    12901 cattaatgcc tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc
    12961 atcagcaccg gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac
    13021 cagaatcggc agtgaaacat cgttgctacg ccagcggcga atcagtgaca gaccgtcctc
    13081 gtcaggcaat ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc
    13141 ggcttctttg gcatcttctg cgtcatcgac ctgatgacca gcatcctgaa tctgaacttt
    13201 aaggtggtga cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc
    13261 ctgtcttaat tattaaaata gtgtaacgcg attatgtgtt tatgggggta aacattaaat
    13321 aaaccagcgg ggaggggagg taaagtgaaa aaagaaaaag tggataatct taataagcag
    13381 gccggacagc atcgccatcc ggcactgatt tgaggcttat ttcagctcat caaccatcgt
    13441 gatagctcga ccaatatagt tagccggcgt catcgctttc aggcgggctt tctcttcttc
    13501 tggcaacgcc agaccatcga taaactgctt catgccttcg gcgtcaacgc gcttaccgcg
    13561 agtaagctct ttcagcttct cgtacggttt ttcgatgcca tagcgacgca taactgtctg
    13621 gattggttct gccagcactt cccagttgtg atccagctca tccagcagat ggtcacggtt
    13681 cacttccagt ttgctcacgc ctttcagggt ggattgatac gcaatcagcg cataaccgat
    13741 acccacgccg aggttacgca gcacggtgga gtcggtcagg tcacgctgcc agcgggaaac
    13801 cggcagtttg cttgccagat gctgcaatac cgcgttggaa aggcccagat tcccttcgga
    13861 gttttcgaag tcgatcgggt taactttatg cggcatggtg gaagaaccaa tctcaccagc
    13921 aatggttttc tgtttgaagt ggttaagggc gatataaccc cagacgtcac ggtcaaagtc
    13981 gatcagaatg gtgttgaagc gcgcaacgca atcaaacagt tcggcaatgt agtcgtgcgg
    14041 ttcaatctgg gtagtgtacg ggttccattg aatacccagc gaggtgacga actcttcgct
    14101 gaactgatgc cagtcaactt ccgggtaagc ggcgatgtgg gcgttatagt taccgaccgc
    14161 gccgttgatt ttgccgagga tctccacctg gttaagctgg cggtgctggc gctccatacg
    14221 gtaggcgacg tttgccatct ctttaccgat ggttgacggc gtggctggct gaccgtgggt
    14281 acgagacagc agcgggatat cgcggtactg aacggcgaga tctttaatgc catcaatcag
    14341 ttgacgccag tacggcagga tcacttcatc acgcgcggtt ttcagcatta atgcgtggga
    14401 gaggttattg atatcttccg aagtacaggc aaagtggatg aattcagaaa ccgcgtgcag
    14461 ttccgggatc tccgccactt tttctttcag gaaatactca accgctttaa cgtcgtggtt
    14521 agtggtacgc tcgatggttt tgatgcgtgc ggcatcttct tcgctgaaat tggcgacaat
    14581 tgcatcaagg taaccgattg cgtcggcagc aaaagcagga acttccttga tcgctgcgtg
    14641 cgcggccagt ttttgcagcc aacgtacttc aacttgtaca cggaatttca gcaaaccata
    14701 ttcgctgaaa atcccgcgca gcgcgctgac tttatcgccg tagcgtccat cgacagggga
    14761 aacggcggtc agtgaggata attccataga tcacaactcc ggggttaaat gagcaagaat
    14821 ttgttttgcc tgagtggtca ggcgattacg agaaaacatc agttgtagac gtccgccgcc
    14881 gacctggtgc cagagcacgg cggcgcgaat gcctgccagc agggttgcgc gaactttcgc
    14941 ctgcacttgt gggctttgca gtacagcagg ggaaccggtg acctgaatgc gcgggccaag
    15001 cgggctgatc acatcaacat agatagcagc catcgcgctc atcagcgttt cggactgtaa
    15061 atcgaagtgt tcgagctggc gttgcaggcc gttgatccgg ttgcccagag tgtcgagcgc
    15121 gcctttcgct gaggagagtt tgcgctcaag caccatcaag ctgagtgtgt agcgggttaa
    15181 ttcggcgttt aagccctggc aactgctggc attgagcacg ccgagcaggg tttccagccc
    15241 gacgcgcagg ttggcttcgc taccgccaaa aaccgccagc gtcgagctgg ggttcatatc
    15301 aataatactg ttgagtgaga cgtgtagcgc atcggcatca caatgcccct ggtgagcgag
    15361 ttgttgcacc aggcgtgccg actgacaaat accggccagg gcgagggtga tgtcatagta
    15421 attctttgcc acgttcactg cttccttgtt ttaagtaaag ataataatca gaccggcagc
    15481 ggcagacgct gctcaataat accgccaccg aggcacactt caccgttata gaagacggca
    15541 gactggcccg gcgtcacggc ggcaaccggc tcatcgaaaa tcactttaat gcgatcgtcg
    15601 tccagcgcct tgacggtgca cggaatgtcg gtctggcgat agcgggtttt taccgtgcaa
    15661 cgcatagtgc cggtgaatgg ttcgcgatcg acccagtgca gctgctgggc aatcaacccg
    15721 acagacatca gccgcgggtg ttcatggccc tgagcgacaa ccagaatgtt gttttcgacg
    15781 tctttgtcca ccacatacca cggttcttcg gtgccttctt tggtgccacc gatacccaga
    15841 cctttacgct gaccgagagt gtgatacatc agcccctggt gctcaccaat ttcatcgcca
    15901 tcgacggtaa tgattttgcc cggttgcgcc gggagataac ggcccaggaa ctcgcggaat
    15961 ttacgttcgc caatgaagca aatgccggta gagtcttttt tcttcgcggt gaccagacca
    16021 agatcttcag caatcttacg cacctgcggt ttttccagtt cgcctaccgg gaacaggctt
    16081 tgcgcaatct gctcatggct gagcgtataa aggaagtagc tctggtcttt attgctgtcc
    16141 agaccacgca gcaggcggct cttgccatcg acatcggccc gacgtacgta atgaccggta
    16201 gcgatataat cggcacctaa atcttcggcg gcaaattcga ggaaggcttt aaatttgatc
    16261 tccttgttgc acagaatatc cggattcggc gtgcgaccgg ctttatattc ggcaaggaac
    16321 agttcgaaga cgttgtccca gtactcggca gcaaagttaa cggtgtgcag ttcaatgccg
    16381 agcttgtcgc agaccgcctg ggcatcagcc agatccgctg ccgctgtgca atattcctca
    16441 ccgtcgtctt cttcccagtt cttcataaac aggccttcga cctgatatcc ctgttgttgc
    16501 aacagccagg cagaaacgga ggaatcgaca ccgccggaca tgccgacgat tacttttttt
    16561 gcggtttcag acattggatc actcacgaca ttgaacttca aggcggcgta ttctatcacg
    16621 cagcctttta gatgacaccc tttgtaaaag gccagttaaa atcgccaatc atctccagcg
    16681 gataacgttg cccgctttga taacaacgaa tactttccgc caccagcggc gaacgaagat
    16741 ttgacgcctg taaaatttct tcggcgctga cccaacggca gcagtcgata tcgctgtcat
    16801 gaggctgggt cgggcatatt tgctcaagct caatggcaaa gaggaaacgc aaaaacggcg
    16861 ttttatctgg cgcaatccac tgatgcatac gaataaagtg ttgcggctgc gcgctgatgc
    16921 cggtttcttc ccacagttca cgggcggcgg cttccactaa ggtttcatcg gcttccagat
    16981 gcccggcagg ttggttccat aacgctttac cattaatcgt ctcttcaacg actaaaaatt
    17041 tgccttctgc gtgcaccacg caagcaacgg taacgtgcgg tttaaacatt ttgcatcctt
    17101 aatctgtcac ttctcgccat tcgccattgg caagattatc caaagagtaa tctcccatcg
    17161 catagcgaat cagtcgcagc gtggggaaac caacatgggc ggtcatgcgg cgcacctggc
    17221 gattacgtcc ttcatataag gtgatcttca gccagctggt gggaatactt ttgcgttcac
    17281 gaattggtgg attccgcggc cataaccacg cgggttcgtc aaccagctcc gcgcctgcgg
    17341 gcagggtagg gccatcattt aaggttacgc cattgcgcaa ggcttcaagt gcgtcttgtg
    17401 tgggaatacc ttccacctgc acataataga tttttccggt gcgtttaccc ggctgggtta
    17461 aacgcgcctg caacgcgccg ttattggtca gcaccagcaa cccttcgcta tcgcggtcaa
    17521 ggcgacctgc tgcataaacg ccctgaaccg ggatgaattc ttttaatgtt ttgcgtccgg
    17581 tttcatcggt gaactgcgga agaacatcgt agggtttatt gaagaggatc acacgcgtgg
    17641 gctggttttc atgtttacgc ctggtagaac gttgtgagct gaatcgctta acctggtgat
    17701 ttctaaaaga agttttttgc atggtatttt cagagattat gaattgccgc attatagcct
    17761 aataacgcgc atctttcatg acggcaaaca atagggtagt attgacaagt caattacaaa
    17821 tcattaacaa aaaattgctc taaagcatcc gtatcgcagg acgcaaacgc atatgcaacg
    17881 tggtggcaga cgagcaaacc agtagcgctc gaaggagagg tgaatggaaa gtaaagtagt
    17941 tgttccggca caaggcaaga agatcaccct gcaaaacggc aaactcaacg ttcctgaaaa
    18001 tccgattatc ccttacattg aaggtgatgg aatcggtgta gatgtaaccc cagccatgct
    18061 gaaagtggtc gacgctgcag tcgagaaagc ctataaaggc gagcgtaaaa tctcctggat
    18121 ggaaatttac accggtgaaa aatccacaca ggtttatggt caggatgtct ggctgcctgc
    18181 tgaaaccctt gatctgattc gtgaatatcg cgttgccatt aaaggcccgc tgaccactcc
    18241 ggttggtggc ggtattcgtt ctctcaacgt tgccctgcgt caggaactgg atctctacat
    18301 ctgcctgcgt ccggtacgtt actatcaggg cactccaagc ccggttaaac accctgaact
    18361 gaccgatatg gttatcttcc gtgaaaactc ggaagacatt tatgcgggta tcgaatggaa
    18421 agctgactct gccgacgccg agaaagtgat taaattcctg cgtgaagaga tgggcgtgaa
    18481 gaaaattcgc ttcccggaac attgcggtat cggtattaag ccgtgttctg aagaaggcac
    18541 caaacgtctg gttcgtgcag cgatcgaata cgcaattgct aacgatcgtg actctgtgac
    18601 cctggtgcac aaaggcaaca tcatgaagtt caccgaaggc gcgtttaaag actggggcta
    18661 ccagctggcg cgtgaagaat ttggcggtga actgatcgac ggcggcccgt ggctgaaagt
    18721 taaaaacccg aataccggca aagagatcgt cattaaagac gtgattgctg atgcattcct
    18781 gcaacagatc ctgctgcgtc cggctgaata tgatgttatc gcctgtatga acctgaacgg
    18841 tgactacatt tctgacgctc tggcagcgca ggttggcggt atcggtatcg cccctggagc
    18901 aaacatcggt gacgaatgcg ccctgtttga agccacccac ggtactgcgc cgaaatacgc
    18961 cggtcaggac aaagtaaacc ctggctctat tattctctcc gctgagatga tgttacgcca
    19021 tatgggctgg actgaagccg cagacctgat tgttaaaggt atggaaggcg cgatcaacgc
    19081 caagaccgta acctatgact tcgagcgtct gatggaaggc gctaaactgc tgaaatgttc
    19141 agagtttggt gatgcgatca tcaagaatat gtaatcacta catgtgttaa atatttaaac
    19201 gggcgtataa cacgcccgtt gttttattta tgtggatatt attaatagca tatcgagcat
//
LOCUS       DBKELA010000085        17386 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.085, whole genome shotgun sequence.
ACCESSION   DBKELA010000085 DBKELA010000000
VERSION     DBKELA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.085"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(43..774)
                     /gene="rlmB"
                     /locus_tag="ACUQR0_002911"
     CDS             complement(43..774)
                     /gene="rlmB"
                     /locus_tag="ACUQR0_002911"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HHV9109862.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(954..3395)
                     /gene="rnr"
                     /locus_tag="ACUQR0_002912"
     CDS             complement(954..3395)
                     /gene="rnr"
                     /locus_tag="ACUQR0_002912"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HHV9109863.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="ACUQR0_002913"
     CDS             complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="ACUQR0_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HHV9109864.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4064..5362)
                     /gene="purA"
                     /locus_tag="ACUQR0_002914"
     CDS             complement(4064..5362)
                     /gene="purA"
                     /locus_tag="ACUQR0_002914"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HHV9109865.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5466..5663)
                     /locus_tag="ACUQR0_002915"
     CDS             complement(5466..5663)
                     /locus_tag="ACUQR0_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HHV9109866.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="ACUQR0_002916"
     CDS             complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="ACUQR0_002916"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HHV9109867.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="ACUQR0_002917"
     CDS             complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="ACUQR0_002917"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HHV9109868.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="ACUQR0_002918"
     CDS             complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="ACUQR0_002918"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HHV9109869.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="ACUQR0_002919"
     CDS             complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="ACUQR0_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HHV9109870.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="ACUQR0_002920"
     CDS             complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="ACUQR0_002920"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HHV9109871.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="ACUQR0_002921"
     CDS             complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="ACUQR0_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HHV9109872.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="ACUQR0_002922"
     CDS             complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="ACUQR0_002922"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HHV9109873.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="ACUQR0_002923"
     CDS             complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="ACUQR0_002923"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HHV9109874.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="ACUQR0_002924"
     CDS             complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="ACUQR0_002924"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HHV9109875.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15982..17121
                     /gene="queG"
                     /locus_tag="ACUQR0_002925"
     CDS             15982..17121
                     /gene="queG"
                     /locus_tag="ACUQR0_002925"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HHV9109876.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17104..17157)
                     /locus_tag="ACUQR0_002926"
     CDS             complement(17104..17157)
                     /locus_tag="ACUQR0_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109877.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3942 a         4959 c         4368 g         4117 t
ORIGIN      
        1 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
       61 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
      121 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
      181 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
      241 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
      301 cgcaaggtta gtcacccgaa tcagcggaac gctttctgcc gcgccgcagg ctactttttt
      361 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacgcctgc
      421 ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
      481 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
      541 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
      601 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
      661 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
      721 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
      781 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa
      841 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
      901 acaaagcgca ttttgtcagc aatctaaccc tcttttttta aagagggtat tgatcactct
      961 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc
     1021 gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc
     1081 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acgacgcttg
     1141 ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
     1201 cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc
     1261 gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc
     1321 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
     1381 accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
     1441 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
     1501 tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
     1561 agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga
     1621 tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa
     1681 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg
     1741 ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct
     1801 gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg
     1861 ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct
     1921 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
     1981 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta
     2041 cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
     2101 tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg
     2161 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
     2221 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
     2281 tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
     2341 cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc
     2401 atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc
     2461 agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag
     2521 ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca
     2581 tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc
     2641 acttcttctt tcagccccgc aacctgttgc tcaacagcct gcggccagat atacggaatt
     2701 tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact
     2761 tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg
     2821 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
     2881 agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca
     2941 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
     3001 tcagcaccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
     3061 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
     3121 tgaccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
     3181 cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
     3241 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
     3301 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
     3361 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
     3421 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
     3481 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
     3541 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
     3601 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
     3661 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
     3721 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
     3781 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
     3841 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
     3901 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
     3961 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
     4021 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt
     4081 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac
     4141 cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat
     4201 ctttcacgcc gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
     4261 ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc ggcatacggt
     4321 aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga
     4381 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
     4441 tacgacgacg acgacccgta gttgcaccga attcgttacc ctgcttgcag aggaactcgc
     4501 cagtttcatc aaacagttcg gccgggaaag gacctgcacc tacacgagtt gagtaagctt
     4561 tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
     4621 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
     4681 gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
     4741 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
     4801 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
     4861 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
     4921 gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc
     4981 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagagcaacg tgataatcaa
     5041 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
     5101 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
     5161 tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
     5221 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
     5281 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
     5341 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
     5401 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
     5461 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
     5521 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
     5581 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
     5641 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
     5701 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
     5761 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
     5821 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
     5881 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcata
     5941 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
     6001 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
     6061 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg
     6121 atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
     6181 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct
     6241 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
     6301 tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt
     6361 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
     6421 ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag
     6481 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
     6541 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagacca
     6601 ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
     6661 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
     6721 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
     6781 gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
     6841 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
     6901 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
     6961 tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca
     7021 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
     7081 gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
     7141 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggtat
     7201 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
     7261 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
     7321 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
     7381 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
     7441 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
     7501 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
     7561 tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt
     7621 cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa
     7681 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
     7741 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
     7801 cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
     7861 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
     7921 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
     7981 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
     8041 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
     8101 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
     8161 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
     8221 agctgataaa aacgactcct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
     8281 gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
     8341 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
     8401 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
     8461 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
     8521 gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat
     8581 gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
     8641 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
     8701 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
     8761 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
     8821 tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac
     8881 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc
     8941 tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc
     9001 agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata
     9061 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
     9121 gggctcaggg catggtcaaa aagaacgacc gaagcaccca tcgctttgac agcttccgca
     9181 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
     9241 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
     9301 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
     9361 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
     9421 tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc
     9481 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggcg
     9541 ttgttactgt gatgagaaac cgggcgagat gggacaacag tagaaatcgc gtgcttgtaa
     9601 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
     9661 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
     9721 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
     9781 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
     9841 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
     9901 tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
     9961 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc
    10021 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    10081 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    10141 atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    10201 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    10261 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    10321 ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg
    10381 ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga
    10441 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    10501 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    10561 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    10621 agcgttcggc ttcgccgtcc cgataaccat ccctttgtaa ataagggcag aatcaacgct
    10681 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    10741 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    10801 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    10861 tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg
    10921 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    10981 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    11041 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    11101 caatacccaa ttccgctagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    11161 aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg
    11221 tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc
    11281 catcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    11341 gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt tgcatcggcg
    11401 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg
    11461 cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag
    11521 ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt
    11581 ccgggatggc acgcggtgcg ggttgtggtt catcgtccag cggtagcggc gtttccagtt
    11641 gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga cgcgactgat
    11701 ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct
    11761 ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc
    11821 ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa
    11881 tttccgccag tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agggtgagat
    11941 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc
    12001 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac
    12061 cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt
    12121 catcaatatg gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    12181 tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc ggtttaaccg
    12241 tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc
    12301 gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    12361 ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag
    12421 ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa
    12481 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    12541 tttccactag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    12601 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    12661 tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    12721 gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt
    12781 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    12841 cgaccagtac tgatgggata tccggcgagc gcagaacgcc aaggctggcg tgttctggtc
    12901 gacgtttatg tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat
    12961 accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt
    13021 cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac agtttctcgt
    13081 actgctccag ccagctggcc atttcactgt tggcgcgacg gttagagagc acccatacgg
    13141 aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg
    13201 cgttttgctt atgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac
    13261 gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    13321 tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca gggtcctgac
    13381 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg
    13441 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    13501 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc
    13561 gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga
    13621 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa
    13681 gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga atcgccttca
    13741 ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt tgtttgatat
    13801 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    13861 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    13921 cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg atgcgataca
    13981 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    14041 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    14101 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    14161 cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt
    14221 acaaatcaaa gtgatagacc attaagttgt cgagcgtata gggttcgacc agcgtataag
    14281 tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg ctaaaggtgg
    14341 ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg
    14401 ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca
    14461 tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    14521 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    14581 gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    14641 ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt tccggcatca
    14701 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    14761 acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca
    14821 gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat cacgcgattg
    14881 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    14941 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg
    15001 ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt
    15061 tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttctc actgcgggtc
    15121 agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat acgaatagcc
    15181 cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa
    15241 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    15301 gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtcctgta
    15361 acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt gatggtgtga
    15421 tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata
    15481 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    15541 tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    15601 tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt
    15661 gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa cgtgacctca
    15721 atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    15781 ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg
    15841 gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg
    15901 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    15961 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa
    16021 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    16081 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    16141 tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc cggtacgctg
    16201 cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    16261 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt
    16321 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    16381 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg
    16441 ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    16501 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    16561 ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    16621 gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc gctcccggaa
    16681 gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc
    16741 ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta
    16801 cacgcaccgg aactcattga gttatttgcc tggagcgaag agaagttttt aaaagtcacg
    16861 gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat cgccgtggca
    16921 ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag
    16981 cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga gagacgaaat
    17041 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa
    17101 gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    17161 aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttgattttta
    17221 acttatttat caataggtta aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    17281 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17341 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaaga
//
LOCUS       DBKELA010000086        17174 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.086, whole genome shotgun sequence.
ACCESSION   DBKELA010000086 DBKELA010000000
VERSION     DBKELA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.086"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..186
                     /locus_tag="ACUQR0_002927"
     CDS             <1..186
                     /locus_tag="ACUQR0_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HHV9109878.1"
                     /translation="YEWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHR
                     VDIGNLHYIDTGAVFGG"
     gene            complement(211..402)
                     /locus_tag="ACUQR0_002928"
     CDS             complement(211..402)
                     /locus_tag="ACUQR0_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HHV9109879.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(507..743)
                     /locus_tag="ACUQR0_002929"
     CDS             complement(507..743)
                     /locus_tag="ACUQR0_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HHV9109880.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(861..2300)
                     /gene="rsmF"
                     /locus_tag="ACUQR0_002930"
     CDS             complement(861..2300)
                     /gene="rsmF"
                     /locus_tag="ACUQR0_002930"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HHV9109881.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2380..5000)
                     /gene="yebT"
                     /locus_tag="ACUQR0_002931"
                     /pseudo
     CDS             complement(2380..5000)
                     /gene="yebT"
                     /locus_tag="ACUQR0_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4969..6252)
                     /gene="yebS"
                     /locus_tag="ACUQR0_002932"
     CDS             complement(4969..6252)
                     /gene="yebS"
                     /locus_tag="ACUQR0_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HHV9109882.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6382..6879
                     /gene="msrC"
                     /locus_tag="ACUQR0_002933"
     CDS             6382..6879
                     /gene="msrC"
                     /locus_tag="ACUQR0_002933"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HHV9109883.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6976..7674
                     /gene="proQ"
                     /locus_tag="ACUQR0_002934"
     CDS             6976..7674
                     /gene="proQ"
                     /locus_tag="ACUQR0_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HHV9109884.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7694..9742
                     /gene="prc"
                     /locus_tag="ACUQR0_002935"
     CDS             7694..9742
                     /gene="prc"
                     /locus_tag="ACUQR0_002935"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HHV9109885.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9934..10815
                     /gene="htpX"
                     /locus_tag="ACUQR0_002936"
     CDS             9934..10815
                     /gene="htpX"
                     /locus_tag="ACUQR0_002936"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HHV9109886.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10861..12234)
                     /locus_tag="ACUQR0_002937"
     CDS             complement(10861..12234)
                     /locus_tag="ACUQR0_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109887.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12411..13202
                     /gene="kdgR"
                     /locus_tag="ACUQR0_002938"
     CDS             12411..13202
                     /gene="kdgR"
                     /locus_tag="ACUQR0_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HHV9109888.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13345..13584)
                     /locus_tag="ACUQR0_002939"
     CDS             complement(13345..13584)
                     /locus_tag="ACUQR0_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HHV9109889.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13743..13886
                     /gene="mgrB"
                     /locus_tag="ACUQR0_002940"
     CDS             13743..13886
                     /gene="mgrB"
                     /locus_tag="ACUQR0_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HHV9109890.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13961..14248
                     /locus_tag="ACUQR0_002941"
     CDS             13961..14248
                     /locus_tag="ACUQR0_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HHV9109891.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14827..14970
                     /locus_tag="ACUQR0_002942"
     CDS             14827..14970
                     /locus_tag="ACUQR0_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HHV9109892.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14983..15192
                     /gene="cspE"
                     /locus_tag="ACUQR0_002943"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14983..15192
                     /gene="cspE"
                     /locus_tag="ACUQR0_002943"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HHV9109893.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15358..16167
                     /gene="rlmA"
                     /locus_tag="ACUQR0_002944"
     CDS             15358..16167
                     /gene="rlmA"
                     /locus_tag="ACUQR0_002944"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HHV9109894.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16164..16730)
                     /gene="mntP"
                     /locus_tag="ACUQR0_002945"
     CDS             complement(16164..16730)
                     /gene="mntP"
                     /locus_tag="ACUQR0_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HHV9109895.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16727..16849)
                     /locus_tag="ACUQR0_002946"
     CDS             complement(16727..16849)
                     /locus_tag="ACUQR0_002946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9109896.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT         4532 a         4156 c         4337 g         4149 t
ORIGIN      
        1 tatgaatggc aaaaggacgt tgatttgcat caggtcttgt ggagccgctc gcgattaggt
       61 gaacgcccaa aagggcaggg aattacaggt gctgatcatt tctggtttgg tcatacaccg
      121 ttgcgacatc gcgtggatat tggcaacctg cattatattg ataccggtgc tgtctttggg
      181 ggctaactga ctcttgtgca actgcaataa ttaaaaatca ccatactcct gtgctggtcg
      241 ccagaaacca tctataaaat cctcaatcgg aaaacaaccg ccatggcgga tccgttgatc
      301 gctcatagaa taaagacact gctgttccgt gttgtagaca tccacaacaa tatcttcaca
      361 accgccatct aggtagcaaa caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg
      421 tgccgtaccg ttaagtttag gagagatttt acaacggggg aataaccagg acaaataacc
      481 cgccatgccg acgggttctt tttggatcag gcaagacgca taatccaggc atcagattgt
      541 tgatcgtaac gggtacggta aaggacagag tgttcgccat ttagcagggc agggatactg
      601 gtttcagcat cccaggcatc cagttgcata cataaatcgg ggtcagattt acaaggaatg
      661 gttaacgttc gatcacccgg cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg
      721 cctatgcgca cactggtttt catcttcgtg ctctccattt agctgctgat tgtagtacga
      781 ccagtccgat cgcacaattt ttcagcctag ttgaggccgg cgaatgtgcc agtcaaaaag
      841 tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa gtttcccgtc ccgcaccagt
      901 tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt tggctaaacc aatcggctga
      961 tgctggaaag taaccaacac gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc
     1021 ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa aagcgtttac attgtcgggg
     1081 gaggcatggg caataaccgc ttcatgctgc cagcgataac ctttgttatg cgtttcggca
     1141 agtttaatcc ctaagcgaga aaatcggact ttaccgatca gggcttcaat gcctaccggg
     1201 aacaaccaca gttctttgtc gcgttgccag agtcgcaggt ttccatccca gtttaagcca
     1261 acacttgcag ccgcctgacg aatttgtccg gcttcgcgat ctttcaccgg gctgaacggg
     1321 aaattgccca ctttgtattt cggggcgggt aaggcgggga tcgcctgagt tttacgcaga
     1381 cgagcaacga agaagccttc gcagtcgtaa atttgtggga aaacatgcaa aaaaccttct
     1441 tcggtcagcg ccttgtttgc accagggaag agatcgccaa gtggtaaaaa ctctacttcg
     1501 tcggggtaag tctctttcag ccaccggcaa acggcttcgt tttcttcccg gtttaaggta
     1561 caggttgagt aaaccagcgt accgccagga cgtaatgcat gaaaggcgct gtcaatcagt
     1621 tcccgttggg tagctgcgat ttcctgattg ctttcgggtg accagttttt tagcgcatcg
     1681 ggatctttac gcaccacgcc ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac
     1741 atttctggca ctgccgcacc aaacacgcgg ccaccaaaat gtgtgagcgc aacattactg
     1801 atgccacagc ggctgatatt ggcatgtaac acttttaccc aactggcgga aaactcattg
     1861 gcaaggattg ccccttcgtt attcatccgc gcggcaattt gcgtcgtttt tgagcctggc
     1921 gcagcagcga catccatcac ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg
     1981 ggcaacattg aactggcttc ctgaatataa aacaggccac ttaaatgctc ggtggtacta
     2041 cccaatggca atgcatcttc attgtcgcgt tcaatccaga aaccttcttc acaccacgga
     2101 attggcgtaa gcgtccagcc ataaggagcg gttaattgca ggaaatcagc aacggagatt
     2161 ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct gacaggcggc aagaaaatca
     2221 tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa
     2281 taaacggtgt gttgggccac gagcatgtac cactggcaaa aaaacaggcg cgcagtttag
     2341 cataagcgct ccggcgcagg cacgccggag cagtgggcat tatttgggaa gcgcagttcc
     2401 ccattcacgc cactctttcg gttcactttc ctgcaacagg aagtgtttgc cttcttgggc
     2461 tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg atgccgccac ggataaattg
     2521 gttaaaggtg ccggttttca ctacgccgcc cgtcagacca aagtccagac tgtaacccga
     2581 tgccaaccag aagacggaat tgttacgcac caggtgttga tagcgtttac tgatgcgcat
     2641 cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt ccggtaaccg tacctacttc
     2701 cagaccacgg aacagcacag gcgtaccgat gcctaacgaa ccggcttccg gcgcttcaac
     2761 aataatgctt aaaccatcta ggtaacgcga atcagtaatg gtggcctctt ctaattcaaa
     2821 gtcgcgacga ggattgccac ggcctggttc gacgttgata tacggctgga ggatagtatc
     2881 tagatgctca acgccagctg ccgagatttg cggtgtgacc actgagaagc gcgtaccacc
     2941 gcgagcaaag gtctggacat attccggata gagcaccgcc tttgcctgta cttcattgcg
     3001 tgcggtaatc agatccagcg tctggatttg cccgatatca ataccgagat agcgaattgg
     3061 catcccgacc gccagttttc cggcatcgaa agcgtgaagc gtaatctgcc tgccaaccgc
     3121 acgggccgct gtttcggaag catacagaat tcgcttgtcg cctttacgct gactggcgct
     3181 ggcaccactg aggttatcga agctaatggc cccctttaat gctctggaga gcggggatgc
     3241 ctgtacggtc aggccactac cattcagctg aactttcgcc ccgccttctg cccagaacac
     3301 gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg tgcagatcga tatcgaacgc
     3361 gttagctcgc ggacgcacgg taataacttc accaacttca aatttacggt acagcactac
     3421 cgatcctgcc tgcacatccg gcagcgtctt tgcactcaaa ctcacggttg tggtgggtaa
     3481 atcgctaagg ctgttctcca gcgctttttc cagattggca tacagtggat agctggcttt
     3541 catctcgcct ttatcgcccg gcagaatacg tatcccgccg ttaatccatt ctgaggcgct
     3601 ggcaccgaga aactcaacgc catccagccc caccttcacg tcgacacggc tgttgacgac
     3661 aaatttgcta tcgcctttta ccagttctcg atgctgaggc tcgatggcga cggtaaaggt
     3721 gacgcctttg ctggtgagtt tacgatcgat aacctggcct acctgcacgc cgtgaagaat
     3781 gagcggctga cccgcatcaa taccgtaact ttccggtgct gtcagggtca gtgtcagaac
     3841 gtccggctct tgcagcaggg ctttttcgcc cggcacaaca acgaactctt tgcgtggctc
     3901 gccatcgccg ggcaccaact caaaggtttt tccggtcagc agggcgctga gattagcatc
     3961 gctaagggat aatttcgggt tgcgtaattc gatgcgggta ttttcacgaa gcagggatca
     4021 acggtcattt ccccagtgac tttaccacca ggatttaaat ccagtttagt cagctgtccg
     4081 acttccagcc cctgatacat taacggcgtc gagtcggcgg ttaatcctgc tccactcggc
     4141 agttccagtt ttattattac tccacgctgg ctgtgggcca gatcttcata cagaccaaag
     4201 gtatcttccg cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta
     4261 accagtgccg ccagactttc cagtttcacc ttcgcgccac tgatactgac gttggcatca
     4321 acgccggaaa cgtttcagaa acggctacct tttttcacca gatcggtaaa acgccgctcg
     4381 atcaggacat caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt
     4441 cccaccggga tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagagcgggg
     4501 gcttgcaggt ggatcatcag atcgccattg tccagccgat atttcggttg ggtatcgagc
     4561 gcgacaaagt gatcctgctc tttaccttta cccggcatca tgccgatata gttcccaccg
     4621 acgagggcgt ccagcccgga gacacctgcc aacgatgctt ttggcgtcat cagccagaac
     4681 tgagtctctt cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc
     4741 ttacgaagat cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa
     4801 cgaacaggcg tacggcccgg aacaatacca tccgccgaca taaagtcgat ggtgacggta
     4861 ttaccccggt cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa
     4921 ggcagcagcc agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa
     4981 gcgggcgttt cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc
     5041 aagaatagtc aaaattacgg cagcgccaaa ataaaacgca gccggtccca tagtaaaagc
     5101 gaggatctga tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat
     5161 cgaccagcgt ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg
     5221 ctggcattta aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg
     5281 taccagaata ctggcgataa acacgattcc cgcaaccgca atgttgctac ttgccagcga
     5341 cataattccg gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat
     5401 aggcaatagg ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt
     5461 ttgcaaacta tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agagaccacg
     5521 ctgatcagga tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg
     5581 cgtagcgggg cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa
     5641 cgtcaccgtc gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat
     5701 atgggcataa tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag
     5761 cattacccac tctttaagtc gctcaagcat cagcagtacc ggacgtaaat tcattcccag
     5821 tcggttacca aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac
     5881 gcagaaaaag accattgatc ccgttatcgc atcgccctgt ttggtcattt gccagatgcc
     5941 ttgcatgacg ttggcgtcaa tacgaatgcc taacagccag atatgcaaca gcggttcgcc
     6001 ccaggcaaac ggcataagca acagcatggt gaaggccatt gccgccaggc gcgttagcga
     6061 ccagtcgcgc ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tttgatgaga
     6121 atttatctcg ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg
     6181 cggcagttcc tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt
     6241 gttaagagcc atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg
     6301 gaatgatctt gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta
     6361 agacaaccaa gtgattaagt aatgaacaaa acagaatttt acgcggattt aaatcgcgac
     6421 tttaacgcgc tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg
     6481 ttgttatacg agcgtctcac tgacgttaac tgggcaggtt tttatttgct tgaggacgat
     6541 acactggtac tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc
     6601 ggggtgtgcg gcacagcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg
     6661 tttgacgggc atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg
     6721 gtgaaaaatc agattattgg tgttctcgac atcgatagta ccgtcttcgc tcgctttaca
     6781 gacgaggacg agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg
     6841 acggattaca aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt
     6901 actggtagcg tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg
     6961 taatcaggaa atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg
     7021 tttctggccg aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa
     7081 attggtattt ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg
     7141 caattacgat ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa
     7201 cccggcgcaa cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat
     7261 gtagagcatg ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct
     7321 gaacagcaag cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg
     7381 cgccgcgaac gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa
     7441 cctcgtgcgc aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa
     7501 cagcacaccc cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa
     7561 gcgggtcaaa acgcgatgga tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc
     7621 cagctgaatt cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga
     7681 ggccgggcca ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat
     7741 agcaggccag accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa
     7801 ggaagagacg cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca
     7861 ttatcgccag ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa
     7921 tctgctcgat tacagccaca atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa
     7981 gaaaaccgag ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta
     8041 caatctggcg caaaagcgcc gttttgaacg ttaccagtac gctttgtcgg tactggaaaa
     8101 gccgatggat ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc
     8161 gaaaaacgag gctgagttga acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag
     8221 cctgaagctg acaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa
     8281 atttgccatt cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac
     8341 ggcgtttgcg cgtgaaatcg atccgcatac caactatctt tccccgcgta ataccgaaca
     8401 gttcaacact gaaatgagtt tgtcactgga aggtattggc gcagtgctgc aaatggatga
     8461 tgactacacc gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat
     8521 cagcgttggt gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgatgtgat
     8581 tggctggcgt cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg
     8641 tctggaaatt ttacctgctg gtaaagggac caagacccgt actgtaacgt tgacccgtga
     8701 acgtattcgt ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa
     8761 agtcggcgtg ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca
     8821 actgcagaaa ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg
     8881 cggtggggcg ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat
     8941 tgttcaggtc cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt
     9001 gttctataaa ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat
     9061 ctttgccgcg gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg
     9121 taaaggcacc gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc
     9181 tgaatggcca gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg
     9241 cggcagtacg caacgtaaag gcgtaacacc agacatcatc atgccgacgg gtaatgaaga
     9301 aacggaaacg ggtgagaaat tcgaagataa cgcgctgccg tgggatagca tcgatgccgc
     9361 gacttatgtg aaatcaggag atttaactgc ctttgaaccg gagctgctga aggaacataa
     9421 tgcgcgtatc gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa
     9481 cgctatgaag gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa
     9541 taatgaagat gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gagaaggtaa
     9601 accggagttg aagaagctgg atgatctatc gaaagattac caggagccgg atccttatct
     9661 ggatgagacg gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga
     9721 acaacccgct cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa
     9781 cagcggcaag atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac
     9841 ttacgcacta tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat
     9901 tgcgttttgt taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta
     9961 acgaacctgg ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg
    10021 agcagcgttc aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt
    10081 tcgcttctga tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa
    10141 ccgcgtaacg aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggcg
    10201 gggatcgcta tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc
    10261 ggtgcgcgcc gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc
    10321 ccggatgaag ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg
    10381 gtcaccatga cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt
    10441 ctggcgcagc ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac
    10501 ggcaacccgc tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg
    10561 gcgagcatta tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg
    10621 gcaaaactgg ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat
    10681 gaaccgcaag aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc
    10741 agtgagttgt tcatgactca cccgccgctg gataaacgta ttgaagctct gcgtacgggt
    10801 gaatacctga agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt
    10861 ttatgccctg gctcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc
    10921 cagaatagcc gcagccatca gcgagacgtg cgtaccatta tcaccaaact gatttagcat
    10981 cagcgccacc agcgccgcgc cactactctg acccagtaga cgagccgttc ctaacatgcc
    11041 actggcccca ccgctacgtt cgcgcggagc ggaggtaata atggtgtggt tattgggtga
    11101 ctggaataag ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc
    11161 aggtgacgcg ggcagcagaa ccagggaaaa aagccccgta gccatgatga acaaccctaa
    11221 agcacccagc aatcctgcat gtacgcgttc aatcaaatag cctgccagcg gagccatcac
    11281 catcgttgct aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag
    11341 cacggtttgc aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca
    11401 aacagatgtg caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag
    11461 cggtacggga agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac
    11521 cagttccgca gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat
    11581 aagcaggccg aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact
    11641 ggcgcgagaa ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa
    11701 cggtacgttg attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat
    11761 tgtcggcccg gcagcagaag agacggcaac aataaacgag tttatgccca tccctctccc
    11821 cagaaaacgt tgtggataga tcaggcggat aagtgcggta ttaacgctca tcaacgctgc
    11881 accgccgaaa ccttgtatga cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc
    11941 gcagaacagt gaagacaaca gaaaaacgac cagaccgcat ttataaatac gtcgatagcc
    12001 aaacatatcg cccagaaacg aaaacgagag cagggagatg acaatggcga tttgataggc
    12061 gttcactacc cagatggaac tggctggtgt ggcatgaagg tccgtggcaa ttgttggcag
    12121 ggcgacgttg gcgattgcgc cgtcaaggac agccatcgaa ataccaatca caatggttaa
    12181 tatcgcaccg tatcgctggg gcaatggcag gccgtcggcc tgaacttttg gcataggaat
    12241 tttatatctt tggtgaataa tcaacagggg tatgattcta gcactgatat tcacggtgga
    12301 tgtcgcagaa ttgtaactaa ccggcaaaag atggattgcg accaccctgg tgcgaaatta
    12361 taataaaaaa cagttctgat ttttataaaa cactcgcaat gaggtgataa atggctaacg
    12421 cagatctgga taaacagcct gattctgtat cttccgtgct aaaagttttt ggcattttgc
    12481 aggcgctggg tgaagagcgc gaaataggga taaccgagct gtcgcagcgc gtcatgatgt
    12541 caaaaagcac cgtttatcgc tttttacaga ccatgaaaac cttaggttat gtggcgcagg
    12601 aaggggagtc ggagaaatat tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt
    12661 tacaaaacgt cgatttaatt cgtagcgcag atatccagat gcgtgagatc tcccgcctga
    12721 ccaaagaaac tatccacctc ggcgcactgg acgaagacag tattgtttac attcacaaaa
    12781 ttgactctat gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca
    12841 gcaccgcgat tggtaaggta ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc
    12901 ttgagggcgt ggagtataaa cgcagtaccg agcggaccat caccagtaca gaagcgttat
    12961 tacccgttct ggaccaggtg cgcgagcagg ggtatggaga agataacgaa gagcaggaag
    13021 aagggctgca atgcattgcg gtaccggtat ttgatcgctt tggcgtggtc attgccggtt
    13081 tgagcatctc cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa
    13141 tgttgcatac cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac tatccgttct
    13201 gatgagagta agaacctgtc ggaatatcaa acagacaggt tctttattta gcatgagaaa
    13261 aataaagttg aaggtgcgtt atattaaacg cgcttgctat aagaatattt tactcatgag
    13321 tgagaatctg gttatttatt acccttaacc attatcgacc acgatattgc ttttacgtaa
    13381 cagtgggcaa tctgttatct ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct
    13441 ggtgcctctg gcagtcagat aggtacattg caaacctaat cctgcggcat tctctttgct
    13501 tccaatcaaa acgccatatc cgctgagtaa taatcctatc cataccagtg ctatcagcat
    13561 aactgtgcga atgatgaatc gcattacaat ctcttctctt tttatgttcg cttaatcgta
    13621 gcggcaatat gcgctgaagc aagctactca ttccgaaaaa gcacgaatat cgacatagtt
    13681 aggcgctgtt taactaacgc atgctagttt aatgacataa ggtaggtgaa gcggagattg
    13741 gagtgaaaaa gtttcgatgg gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt
    13801 gggcgcaggt attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg
    13861 ccattaacca gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc
    13921 cagcagtggt agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc
    13981 aggcgtgatg aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat
    14041 cttgtggttt tttatcaatc gtgccagttc acggactaac gaacagattg aactgcttga
    14101 ggcgttgctg gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa
    14161 cgaaccagag aaagcagata aaaagaccat tgagagtcaa aaatcggttg atgacgaaga
    14221 tattattcgc ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg
    14281 aagggttact caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa
    14341 aactctggtc tgacttcaaa taattatcat attatttatt agcgaaacgt ttctctcttg
    14401 atttttttgc tcactcatca atttttctta ttttaaattt acaatccctt tggggattga
    14461 cttctcttta gggtaattga tagccgttaa ctgactgttt tatgagaaaa agtgatataa
    14521 ctttttattc attgcatagc agaaaatgtg atattgcacg cactatgtaa taacttctcc
    14581 ctctggcctg gaacaactga acttattgaa ctatgttaga aactacgcca gtttaagtat
    14641 ctgcctgaac tggcaaggtt aagcacaatg atatatcggc gcgtattccg ttgcataagt
    14701 gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa
    14761 tggcttgcca taattaacgt tgtatgtgat aaaagatttc gggttaaacg aggtacagtt
    14821 ctgtttatgt gtggcatttt cagtaaagaa gtcctgagta aacacgttga cgttgaatac
    14881 cgcttctctg ccgagcctta tattggtgcc tcatgcagta atgtgtcagt tttatctatg
    14941 ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa gattaaaggt
    15001 caggttaagt ggttcaacga gtctaaaggt tttggcttca ttactccggc tgatggcagc
    15061 aaagatgtgt tcgtacactt ctccgctatc cagggtaatg gcttcaaaac tctggctgaa
    15121 ggtcagaacg ttgagttcga aattcaggac ggccagaaag gtccggcagc tgttaacgta
    15181 acagctatct gatcgaatcc actgatctga agtgtgaata cgcttcaatc tcgctataaa
    15241 gcctcgtcga atgcgaggct ttttactatg ctttatcttc gctcctggcg ttcggatatt
    15301 tgcccgccgc gtgattcgcg ttacacttgc ggcctttagt atcctgctgg agttgtcatg
    15361 tcgttttcct gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt
    15421 ccccaacgac atcagtttga tatggcgaaa gaagggtatg tcaatctgct gcccgttcag
    15481 cataaacggt ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg ccgcgcattc
    15541 ttagatgccg gacattatca gccgctgcgt gatgcaattg tcgcccaact gcgggaacgg
    15601 cttgatgaaa aggccacggc ggtgctggat attggctgtg gtgaagggta ttacacacac
    15661 gcatttgccg atgcgttgcc cgaaatcacc acgtttggcc tggatgtctc gaaggtagcg
    15721 ataaaagcgg cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc cagccaccgt
    15781 ttgccgtttt ccgataccag tatggacgcc ataatacgta tttacgcgcc gtgtaaagca
    15841 gaagaattag cacgagtagt gaagcccggc ggctgggtca ttactgccac gccgggaccg
    15901 cgacatttga tggagctgaa ggggctgatt tacaatgaag tacatcttca tgcacctcat
    15961 gcagaacaac tggaaggttt tacattacag cagagtgcgg agttgtgtta tccgatgcgt
    16021 cttcgcggtg atgaagccgt cgcattattg cagatgacgc cgtttgcctg gcgtgcgaag
    16081 ccagaagtct ggcaaacact ggcagcaaaa gaagtgttcg actgccagac ggactttaat
    16141 attcacctct ggcagcgttc ttattaaccg tggaagtgcg tccagaggat ctggacgccg
    16201 atgccgatca gcaccagccc gccgagaatt tccgcttttt tcccaataat tgagccgata
    16261 aagcgaccaa ccatcatccc taatgtcgac ataatcaagg ttgcacaacc aatggccaat
    16321 gcggtcgtga taatgttgac ctgaaggaaa gcaagaccaa cacccacagc catggcatcc
    16381 aggctggtgg caatcgcggt ggttaccagt agccagaaac cgtgccgacg gcgcggctct
    16441 tcatcttcat catctgcgcc acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc
    16501 agcagcacaa acgcaatcca gtgattccat tcaaggacaa accggctggc taacatgccc
    16561 attccccagc cgatcagcgg cgtcagggtt tcgacggcac caaaaataag gccggttcgc
    16621 aatgcttcag aaaatttcgg tttatggagg ggggcacctt taccgattga tgcagcaaat
    16681 gcatccatcg acataccaaa cgcaagaaga acagtagcag tgatattcat gacaacgtcc
    16741 tgaccggggt atccataaaa cacatcatcg cccccagcaa acatgcatac atgaacaaca
    16801 agccgcgatg ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc
    16861 tgactgaaag tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca
    16921 tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa catattttga
    16981 gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac gattttcatt
    17041 tatgtttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga gataatatag
    17101 cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa attcataagt
    17161 tattgagttg atac
//
LOCUS       DBKELA010000087        17144 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.087, whole genome shotgun sequence.
ACCESSION   DBKELA010000087 DBKELA010000000
VERSION     DBKELA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.087"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(88..306)
                     /locus_tag="ACUQR0_002947"
                     /pseudo
     CDS             complement(88..306)
                     /locus_tag="ACUQR0_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            616..2388
                     /gene="aspS"
                     /locus_tag="ACUQR0_002948"
     CDS             616..2388
                     /gene="aspS"
                     /locus_tag="ACUQR0_002948"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HHV9109897.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2506..2958
                     /gene="nudB"
                     /locus_tag="ACUQR0_002949"
     CDS             2506..2958
                     /gene="nudB"
                     /locus_tag="ACUQR0_002949"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HHV9109898.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2987..3727
                     /locus_tag="ACUQR0_002950"
     CDS             2987..3727
                     /locus_tag="ACUQR0_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHV9109899.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3762..4283
                     /gene="ruvC"
                     /locus_tag="ACUQR0_002951"
     CDS             3762..4283
                     /gene="ruvC"
                     /locus_tag="ACUQR0_002951"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HHV9109900.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4285..4887)
                     /locus_tag="ACUQR0_002952"
     CDS             complement(4285..4887)
                     /locus_tag="ACUQR0_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HHV9109901.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4956..5021
                     /gene="yobI"
                     /locus_tag="ACUQR0_002953"
     CDS             4956..5021
                     /gene="yobI"
                     /locus_tag="ACUQR0_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HHV9109902.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5160..5771
                     /gene="ruvA"
                     /locus_tag="ACUQR0_002954"
     CDS             5160..5771
                     /gene="ruvA"
                     /locus_tag="ACUQR0_002954"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HHV9109903.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5780..6790
                     /gene="ruvB"
                     /locus_tag="ACUQR0_002955"
     CDS             5780..6790
                     /gene="ruvB"
                     /locus_tag="ACUQR0_002955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HHV9109904.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="ACUQR0_002956"
     CDS             complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="ACUQR0_002956"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HHV9109905.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="ACUQR0_002957"
     CDS             complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="ACUQR0_002957"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HHV9109906.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8553..9485
                     /gene="znuA"
                     /locus_tag="ACUQR0_002958"
     CDS             8553..9485
                     /gene="znuA"
                     /locus_tag="ACUQR0_002958"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HHV9109907.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9501..10823
                     /gene="mepM"
                     /locus_tag="ACUQR0_002959"
     CDS             9501..10823
                     /gene="mepM"
                     /locus_tag="ACUQR0_002959"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HHV9109908.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10943..11914
                     /gene="lpxM"
                     /locus_tag="ACUQR0_002960"
                     /gene_synonym="msbB"
     CDS             10943..11914
                     /gene="lpxM"
                     /locus_tag="ACUQR0_002960"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HHV9109909.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="ACUQR0_002961"
     CDS             complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="ACUQR0_002961"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HHV9109910.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="ACUQR0_002962"
                     /gene_synonym="yebK"
     CDS             complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="ACUQR0_002962"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HHV9109911.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14821..16296
                     /gene="zwf"
                     /locus_tag="ACUQR0_002963"
     CDS             14821..16296
                     /gene="zwf"
                     /locus_tag="ACUQR0_002963"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HHV9109912.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQVLCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16531..>17144
                     /locus_tag="ACUQR0_002964"
     CDS             16531..>17144
                     /locus_tag="ACUQR0_002964"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HHV9109913.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDR"
BASE COUNT         4374 a         4228 c         4464 g         4078 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg ggcatcaacc ggctgtttta atgcttcggc
      121 gtaatcggca gaccagccaa ctcgcaccag aaacacgggc tgaccgctgg cgcgaaattt
      181 cgccgccagc ttcccggcgc gattaactac ctcatcggca gtatgtggac caccggcaaa
      241 aggtaagatg ccttcttgta aatcgatcac caccaacgca gtggttttag cattaagttc
      301 aagcatgctg actcctgaaa aaacaaaatc gtgcctcaca ccttaatgcg attagccgat
      361 ggcggaacga caaattttgt taatttttat gagttcgggc aacaccttta tagcaaactg
      421 gcgtagagtc tgatttcagg cccgatataa agtggtaacg aggcgttcca gcgcgggcca
      481 ggctcttacg gtgcggcaga atttccagta taatagccgc cttttttcat tcagttgtga
      541 catacagcta acgctgcgac ttggtcacct gcgcagcagg gggcatccgc cggcagctaa
      601 gttaagggat atctcatgcg tacagaatat tgtggacagc tccgtttgtc ccacgtgggg
      661 cagcaggtga ctctgtgtgg ttgggtcaac cgtcgtcgtg atcttggtag cctgatcttc
      721 atcgatatgc gcgaccgcga aggtatcgtg caggtatttt tcgatccgga tcgtgcggac
      781 gcgttaaagc tggcctctga actgcgtaat gagttctgca ttcaggttac gggcaccgta
      841 cgtgcgcgtg acgaaaaaaa tattaaccgc gatatggcga ccggcgaaat tgaagtgctg
      901 gcgtcctcgc tgactatcat caaccgcgca gatgttctgc cgcttgactc taaccacgtc
      961 aacaccgaag aagcgcgtct gaaataccgc tacctcgacc tgcgtcgccc ggaaatggcg
     1021 cagcgcctga aaacccgcgc taaaatcacc agcctggtgc gccgttttat ggatgaccac
     1081 ggcttcctcg acatcgaaac tccgatgctg accaaagcca cgccggaagg cgcgcgtgac
     1141 tacttggtgc cttctcgtgt gcacaaaggt aaattctacg cactgccgca atccccacag
     1201 ttgttcaaac agctgctgat gatgtccggt tttgaccgtt actatcagat cgttaaatgc
     1261 ttccgtgacg aagacctgcg cgctgatcgt cagcctgaat ttactcagat cgatgtggaa
     1321 acttctttca tgaccgcgcc gcaagtgcgt gaagtgatgg aagcgctggt gcgtcatttg
     1381 tggctggaag tgaagggcgt ggatctgggc gatttcccgg taatgacctt tgcggaagca
     1441 gaacgccgtt atggttctga taaaccggat ctgcgtaacc cgatggaact gaccgacgtt
     1501 gccgatctgc tgaaatctgt tgagtttgca gtatttgcgg ggccggcgaa cgatccgaaa
     1561 ggccgtgttg ccgctctacg tgttccgggt ggcgcatcgc tgacccgtaa gcagatcgac
     1621 gaatacggta acttcgttaa aatctacggc gcgaaaggtc tggcttacat caaagttaac
     1681 gaacgcgcga aaggtctgga aggtatcaac agcccggtag cgaagttcct taatgcagaa
     1741 atcatcgaag ccatcctgga tcgtactgcc gcgcaagatg gcgatatgat tttcttcggt
     1801 gccgacaaca agaaaattgt tgccgacggg atgggcgcac tgctcctgaa agtgggtaaa
     1861 gaccttggtc tgaccgacga aagcaaatgg gcaccgctgt gggttatcga cttcccgatg
     1921 tttgaagacg acggtgaagg cggcctgacg gcaatgcacc atccgttcac ctcaccgaaa
     1981 gatatgactg ctgcagaact gaaagctgca ccggaaaatg cggtggcgaa cgcttacgat
     2041 atggtcatca atggttacga agtgggcggt ggttcagtac gtatccataa tggtgatatg
     2101 cagcagacgg tgtttggtat tctgggtatc aacgaagagg aacagcgcga gaaattcggc
     2161 ttcctgctcg acgccctgaa atacggtact ccgccgcacg caggtctggc attcggtctt
     2221 gaccgtctga ccatgctgct gactggcacc gacaatatcc gtgacgttat cgccttcccg
     2281 aaaaccacgg cggcagcgtg tctgatgact gaagcaccga gctttgctaa cccgactgca
     2341 ctggctgagc tgagcattca ggttgtgaag aaggctgaga ataactgata tgactcaaat
     2401 acacgaaatc attcgcgttg catcaaggcg gcaactgagt gaactcccat gagcatagat
     2461 aactatgtga atgggatgag cgaaggcagt caacgaagag gcagcgtgaa ggataaagtg
     2521 tataagcgtc ccgtttcgat cttagtggtc atctatgcac aagatacgaa acgggtgctg
     2581 atgttgcagc ggcgtgacga tcccgatttc tggcagtcgg taaccggcag cgtggaagag
     2641 ggtgaaaccg cgccgcaagc tgccatgcgc gaagtaaagg aagaggttac cattgatgtt
     2701 gtcgctgaac aactgacctt aattgactgt cagcgcacgg tagagtttga aattttttca
     2761 catttacgtc atcgctatgc gccgggcgtg acgcgtaata cggaatcatg gttctgtctt
     2821 gcgcttccgc acgagcggca gatcgttttc actgaacatc tggcttacaa gtggcttgat
     2881 gcgcctgctg cggcggcgct cactaagtcc tggagcaacc ggcaggcgat tgaacagttt
     2941 gtaattaacg ctgcctgaac aggcagggct atctggagat atttttatgg caggtcatag
     3001 taaatgggcc aacaccagac atcgtaaagc tgcgcaggat gctaagcgcg gtaaaatctt
     3061 cactaaaatc attcgtgagc tggtaaccgc ggctaagctg ggcggtggcg atccggacgc
     3121 taacccgcgt ctgcgtgcgg cggtggataa agcactgtct aacaacatga cccgtgacac
     3181 actgaaccgc gcaattgcac gtggtgtggg cggtgatgat gatgcaaaca tggaaaccat
     3241 catctacgaa ggttacggtc ctggcggcac ggcaatcatg attgaatgtc tgtctgacaa
     3301 ccgcaaccgt accgttgctg aagtgcgtca cgcattcagc aaatgtggcg gtaacctcgg
     3361 cactgacggt tccgtagctt atctgttcag caaaaaaggc gtgatctcct tcgagaaagg
     3421 cgacgaagac accatcatgg aagcagctct ggaagcaggt gctgaagacg ttgtgacata
     3481 tgatgacggc gcgattgatg tctacactgc atgggaagaa atgggtaaag tgcgcgacgc
     3541 tctggaagcg gcaggtctga aagcagacag cgcggaagtt tccatgatcc cgtccaccaa
     3601 agctgatatg gatgcagaaa ccgcgccgaa actgatgcgt ctgatcgata tgctggaaga
     3661 ttgcgacgac gtgcaggaag tttaccataa cggtgaaatc tctgatgagg ttgcagcgac
     3721 tctctgatga tgcctgctaa acagcaaaac ggagacgcgt gatggctatt attctcggca
     3781 ttgacccggg ttcgcgcgtg accggctacg gcgtcatccg ccaggtaggt aggcaactgt
     3841 cctacctggg tagcggatgc atacgcacca aagtggatga tttaccgtct cgtctgaagc
     3901 tcatctatgc gggcgtgacg gaaatcatca cccagttcca gcctgattat ttcgccattg
     3961 aacaagtctt tatggcgaag aacgctgact cagccctgaa actgggccag gcgcgcggcg
     4021 tggcgattgt tgcggcggtg aatcaggaat tgccggtatt tgaatacgcg gcacgtcagg
     4081 taaagcaaac ggtggtaggt atgggtagtg ccgaaaaaag ccaggtgcag catatggtcc
     4141 gcaccttgct gaaactgccc gctaatccac aggcggatgc cgccgatgcg ctggcgattg
     4201 ccatcaccca ctgccacgtt agtcagaatg cgatgcagat gagcgaatcg cggctgaacc
     4261 tgacgagagg gcgactgcgt taagttatac cgcctcggtc agttccggct gaggcgtttc
     4321 aactccctcc gcgtgaatca acaccaaacc cggatgatgc cacaaactgg ctggcgtgac
     4381 ggttttacgc tcccacggaa tagaacctaa gaaccagaat ttccagaaag tgagttttgc
     4441 attcggattg ctatttaaca gttgtccaaa cgtctctatt tcacccaccg gaatacatag
     4501 cgcctcttta taaatatcaa agacaaattt tgaacagaac tggcgcgaag attcgtattt
     4561 aaaacccgtg tggtaaagtt tgcgtagccg ggagggaacc tgttcaacaa ttcgttgttt
     4621 ttgttgttct gtcagcccag cgtctaatcg ctttatagca tagcgttgat tagacgaacg
     4681 tttaataaaa cgagatagcg tggtgatggt tgaaagggga acccggcttt ctgcaaccag
     4741 gaagtcttca ccgttatgac cgataatgat cccgacgtga ttactccagc aatttgatgc
     4801 agctgatatt tgaccaaata aggcagcacc tatacatgta aagacgatat ctccaatttc
     4861 atattcagca gggtaattaa tattcacgtt gtcattccat tgaaatagat acgaatatac
     4921 gtaatagcat aaaaaattat ttttcaggag tcgcaatgta tatttttatt acgcatttct
     4981 tcactgaata tgtaatattc aaatatttgc ttccaatata acctgttgaa taaattatac
     5041 tgtgccattt ttcagatcat cgggacacct cgcaagtttt cttcatcctt cgctggatat
     5101 ctatccagca tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg
     5161 tgataggcag actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag
     5221 tgggcggcgt aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag
     5281 cgggtcagga agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt
     5341 acggttttaa caataaacaa gaacgcacgt tgttcaaaga gttgatcaaa accaacggcg
     5401 tcggcccgaa gttggccctg gcgatcctct ccggaatgtc agcgcagcag ttcgttaatg
     5461 ccgttgagcg tgaagaagtg ggggcactgg tgaaactgcc gggtattggc aaaaaaaccg
     5521 ccgaacgctt gattgttgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta
     5581 cgccagccgc cgacctggta ctcacgtctc ctgctagccc ggcgaccgac gatgctgaac
     5641 aagaagcagt tgccgcgctg gtggcgctag gctataaacc acaagaagca agccgcatgg
     5701 tgagcaaaat cgctcgccct gacaccagca gtgaaacatt aattcgcgaa gccctacgcg
     5761 ccgcgttatg aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt
     5821 gccggaagat gtagcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca
     5881 gccgcaggtt cgttcacaga tggagatttt catcaaagca gcgaaactgc gcggcgatgc
     5941 cctcgatcat ttgttgattt ttggtcctcc ggggttgggt aaaactacgc ttgccaacat
     6001 tgtcgccaat gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc
     6061 gggcgatttg gctgcgatgc tcactaacct tgaaccgcat gacgtgctgt ttattgatga
     6121 gatccaccgt ttatcgccag ttgttgaaga agtgctgtac ccggcaatgg aagactacca
     6181 actggatatc atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc
     6241 gtttaccctg attggcgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgaccg
     6301 ttttggtatt gtgcaacgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag
     6361 tcgcagtgca cgctttatgg ggcttgagat gagtgatgac ggtgcgcagg aagtagctcg
     6421 tcgcgctcgc ggtacgccac gtattgccaa ccgtctgctg cgtcgagtgc gtgatttcgc
     6481 cgaagtgaag cacgatggca ccatctcggc agatatcgct gctcaggcgc tggatatgtt
     6541 gaatgtcgat gctgaaggtt tcgattatat ggaccgcaaa ttgttgctgg cggtaatcga
     6601 taagttcttt ggtgggccgg taggtctgga taacctggcg gcagccattg gcgaagaacg
     6661 tgaaaccatt gaagatgtac tggaacctta tttgattcag caaggctttt tgcagcgtac
     6721 accgcgcggg cgtatggcga cgacgcgggc gtggaatcac tttggcataa cgccgccaga
     6781 aatgccgtaa gtcggattgg tcgctcgcgc cgcatccgac actggcagtg tgtctgatgc
     6841 gacactgttc gcgtcttatc aggccagcca gagatagcga accgtaggtc ggataaggcg
     6901 ctcgcgccgc atccgacaaa tgtgttcagc gatagattag ctggcctgct ttttcatcat
     6961 actgataata aataacagtg ccgcacatag caccaccgac ggacccgccg gcgtatcgta
     7021 aaacgcggaa aaggttaaac cgccagttac tgccaccatc cccaccaaaa cagcgacacc
     7081 agccatctgt tccggcgtac gggcaaagcg acgcgcagta gctgcaggaa taatcagcaa
     7141 cgaagtaata atcaacgcac cgacaaattt catcgctaca ccaatcgtca atgccgtcac
     7201 cagcatcaac aacaatttca cttgctgtaa tttcacacca tcaacaaacg ccagatccgg
     7261 gctaatcgtc atcgacagca aattgcgcca ttgccagaac aaaatagcca ccacgatgac
     7321 cacgccaatc gcaatagaga tgagatcttc tggcgtcact gccagtaaat caccgaacag
     7381 gtaagccatc aaatcaacac gaatgttaga catcagacta acgaccacta ggcccagcga
     7441 cagggcactg tgcgccataa tccctaataa cgtgtcgatc gccagctgtg gacgcttctc
     7501 cagccatacc agaccgcccg ccagcagcag cgtaacggca atcaccgcat agaatggatt
     7561 cacgtccagc aacaaaccaa acgcgacacc aagtaatgag gcatgagcca gcgtatcacc
     7621 gaaataagac atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag
     7681 catgatcccg gctaaccaac cgggaaataa taattcaatc atgagcgatc atttccccga
     7741 cgcaaaacaa ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc
     7801 agttgttcag caccacgagg accaaacatt gaaataaact ccggatgcag ggaaacaact
     7861 tccggtgtgc cggaacaaca aatgtggtga ttcaggcaca gcacttcatc ggtttttgcc
     7921 atcaccagat gcagatcgtg agaaaccatt aaaacgccac aatccagttc acgacgcaac
     7981 tggtcaataa ggtcatataa cgccacttgg ccatttacat ccacgccttg agtgggttca
     8041 tccagcacta ataattgcgg gcaatttaac aatgctcgcg ctaatagtac acgctgcgtt
     8101 tcgccaccag agagcttttg catcggtgcg ttaatcaggt gcccggcctg gacacgtttc
     8161 agtgcaggca aaatatcttc tttatgtgta ccagggcgta agcgtaaaaa acggtttacg
     8221 gtcagtggca acgtggtgtc gagatacagc ttctgcggta catagccgat gcgcagtttt
     8281 ccgttgcgct tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt
     8341 gtcgacttac ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc
     8401 agcgacacat cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa
     8461 accagacttg tcatgttaat tttagtcttg cagtagtcat gaaatgttat aatatcacac
     8521 ttctcatatt cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg
     8581 cagcattatc cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg
     8641 cttcgcttaa acccgttggg ttcatcgctt ctgccattgc tgatggtgta acagaaacgg
     8701 aggttttact gcctgacggc gcttcagaac atgattattc actgcgtcca tcggatgtaa
     8761 aacgcttaca gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgtttatgc
     8821 aaaaaccggt aagcaaatta ccagaagcga agcaggtaac gattgcgcag cttgaagatg
     8881 tgaaaccgct actgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa
     8941 aaagtgacga agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga
     9001 tagcgcgggc tacagcggtt gcaatccatg gaaaattagt ggaacttatg ccgcaaagtc
     9061 gagccaaact tgacgccaac ctgaaggatt ttgaggcaca attggcctca accgaaacgc
     9121 aggttggtaa cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt
     9181 acggctattt cgaaaaacag ttcggactga caccgcttgg tcattttacc gttaaccctg
     9241 agattcaacc tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag
     9301 caacctgcgt ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgcacgag
     9361 ggacatccgt tcgtatggga acgttggatc ccctggggac gaatatcaaa ctgggtaaaa
     9421 caagctattc agaattcctg agtcaattag ccaaccagta tgcgagctgc ctgaaaggag
     9481 attaatgagg aagtgattac gtgcaacaga tagcccgctc tgtcgccttg gcgtttaata
     9541 atttaccgcg accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg
     9601 ctgtctggcg gccctatgtt tatcaccgtg atgccacgcc aattgtcaaa accattgagc
     9661 tggaacagaa cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagctg
     9721 cacaagaaga tgaagccatt ccccaggacg aactggatga caaaatcgcc ggtgaagcgg
     9781 gcgtgcatga atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg
     9841 gtattgatat gggtgatatc acccaacttg ctgctgccga caaagaattg cgtaacctga
     9901 aaatcggtca acaactctcc tggacattaa ccgcggacgg cgaactgcag cgcctcacct
     9961 gggaagtgtc tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa
    10021 tgaccagcga aatgcagcaa ggagagtggg ttaacaatct gctgaaaggt accgtcggag
    10081 ggagctttgt tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga
    10141 ttaaagccat gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg
    10201 tattaatgtc tcgagaaatg cttgatggta aacgtgagca aagccagctg ctgggcgtac
    10261 gtttgcgttc agaaggtaaa gattattacg caatccgcgc tgaggatggc aaattctacg
    10321 atcgtaacgg tactggtctg gcgaaaggat tcttgcgatt cccgacggcg aaacagttcc
    10381 gtatctcgtc taactttaac ccgcgtcgaa ctaatccggt gacaggtcgc gttgcaccac
    10441 acagaggtgt tgatttcgcc atgccacaag gtacgccagt gctttcagtg ggtgacggtg
    10501 aagtggtggt tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc
    10561 gcagctacac cacgcgttat atgcacttgc gcaagattct ggtgaaaccg ggacagaagg
    10621 tgaaacgtgg cgaccgtatc gcgctttccg gtaataccgg acgttcaact gggccgcatc
    10681 tgcactatga agtatggata aaccagcagg ccgtaaaccc gctgacggca aaactgccgc
    10741 gtaccgaagg gctgaccggc tccgatcgtc gcgaattcct ggcgcaggcc aaagagattg
    10801 tgccgcagct acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg
    10861 gcttttttta tttggtgcgg ggcaagttgt gccgctacac tatcaccaga ttgatttttg
    10921 ccttatccga aactggaaaa gcatggaaac gaaaaaaaat aatagcgaat acattcctga
    10981 gtttgataaa tcctttcgcc acccgcgcta ctggggagca tggctgggtg tagcagcgat
    11041 ggcgggtatc gctttaacgc cgccaaagtt ccgtgatccc attctggcac ggctgggacg
    11101 ttttgccgga cgactgggaa aaagctcacg ccgtcgtgcg ttaatcaatc tgtcgctctg
    11161 ctttccagaa cgtagtgaag ctgaacgcga agcgattgtt gatgagatgt ttgccaccgc
    11221 gccgcaagcg atggcaatga tggctgagtt ggcaatacgc gggccggaga aaattcagcc
    11281 gcgcgttggc tggcaagggc tggagatcat cgaagagatg cggcgtaata acgagaaagt
    11341 tatctttctg gtgccgcacg gttgggccgt cgatattcct gccatgctga tggcctcgca
    11401 agggcagaaa atggcagcga tgttccataa tcagggcaac ccggtttttg attatgtctg
    11461 gaacacggtg cgtcgtcgct ttggcggtcg tctgcatgcg agaaatgacg gtattaaacc
    11521 attcatccag tcggtacgtc aggggtactg gggatattat ttacccgatc aggatcatgg
    11581 cccagagcac agcgaatttg tggatttctt tgccacctat aaagcgacgt tgcccgcgat
    11641 tggtcgtttg atgaaagtat gccgtgcgcg cgttgtaccg ctgtttccga tttatgatgg
    11701 caagacgcat cgtctgacga ttcaggtgcg cccaccgatg gatgatctgt tagaggcgga
    11761 tgatcatacg attgagcggc ggatgaatga agaagtcgag atttttgttg gtccgcgacc
    11821 agaacaatac acctggatac taaaattgct gaaaactcgc aaaccgggcg aaatccagcc
    11881 gtataagcgc aaagatcttt atcccatcaa ataaaaaagc ctctcgcgag gagaggcctt
    11941 cgcctgatga taagttcaag tttgcttcag aatattcgaa atctgttgaa ctatcattga
    12001 actataggcc ggatgtggcg ttttcgccgc atccggcaac gtacttactc taccgttaaa
    12061 atacgcgtgg tattagtaga acccacggta ctcatcacgt cgccttgggt gacaatcacc
    12121 aggtcaccag acatcaagta acctttatcg cgcagcagat taaccgcttc gctggcagct
    12181 gctacgccgt cattagcgct atcaaagtgc accggcgtaa cgccacgata gagagcagtc
    12241 aggttcagcg tacgttcatg gcgcgacatg gcgaaaattg gtagaccaga gctgatacgg
    12301 gaggtcatca gcgcggtacg acccgattcg gtcatggtaa tgatcgccgt aacgcccttc
    12361 aggtggttag ctgcgtacat tgccgacatg gcaatagctt cttccacatt gtcgaactga
    12421 acgtccagac ggtgtttaga aacgttgatg ctcgggattt tttccgcacc caggcaaacg
    12481 cgcgccatgg ctgcaacagt ttctgacgga tactgcccag cggcagtttc tgcagacagc
    12541 atcacagcgt cagtaccatc cagaacggcg tttgctacgt ccatgacttc tgcacgcgtc
    12601 ggcatcgggt tagtaatcat tgactccatc atctgggtcg ccgtgattac cgctcggttt
    12661 agctgacgcg cacgacggat caacgctttc tgaatgccga ccagttccgg gtcgccaatt
    12721 tccacaccga ggtcgccacg tgcaaccatt accacgtcag aggcgaggat gatgtcatcc
    12781 attgcatcct ggctgcaaac ggcttccgca cgttcaacct tggcaacaat tttcgcatca
    12841 catcctgcat cgcgtgccag gcgacgggca tagttcagat cttcgccaca gcgtgggaag
    12901 gagacagcca ggtaatctac gccaatcaac gccgcagtct taatgtctgc tttgtctttt
    12961 tcggtcagcg cttcagccga caaaccgccg ccaagtttgt tgataccttt attgttggag
    13021 aggggaccac cgacggtgac ttcggtgaac actttcatgc cctgaacttc cagtactttt
    13081 aactggacgc gaccatcgtc cagcagcagg atgtcaccag gcacgacgtc agcaggcagg
    13141 cctttgtagt cgataccgac tttttctttg tcgccttcac ctttacccag gttggcgtcg
    13201 agcaggaatt tatccccaat attgaggaaa actttgcctt ctttaaaggt ggatacacgg
    13261 attttgggcc cctggaggtc acccagaata gccacatgac gccccagttt tgcggcaatc
    13321 tcacgaactt tatccgcgcg cattttgtga tcttcaggcg agccgtgaga aaagttcata
    13381 cgtacaacgt tggcacccgc cgcgataact ttttcaagat tattatcgcg atctgttgct
    13441 gggcctaacg tggtaacgat ttttgttctg cgaagccttt tggacatgta atactccgtt
    13501 gactgaaaca accaggtgtt gcttgaacat gaaatccgaa tgaaataacg ccgcgatggc
    13561 gataaattaa tctcattcgg atgtcgtata agattaggac agtgacagtc gcttttagcg
    13621 atcgtcactt aaattaagta actgcttatc aaaacgcgat tccttcagcg cttccttgac
    13681 ccgcttcaag ttatctctga attttgcacc gcgtcgcaaa gtaaatcctg tcgccagcac
    13741 atctatcacg gtcagctgtg caagtcgaga aaccatgggc atataaatgt cagtatcttc
    13801 cggtacgtcg agggtaattg ccagcgttgc ttcccgggcg agcggggtac ccgcagaggt
    13861 gagggcaatc accatggcgt cgttttcgcg tgccagctgc gccagctcga ccagattttt
    13921 tgttcttcct gtgtgagaaa tcagcaccac cacgtctccg tcgctacaat tcatacaact
    13981 catgcgttgc agcacgatat catcggagta caccaccgga acattaaaac gaaagaactt
    14041 attcatcgca tcgtgggcaa cggcggcaga agagcctaat ccgaaaaagg cgattttttt
    14101 tgcctgagtg agcaagtcga cggcgcggtt gatggcagat ttatccagtg aatgacggac
    14161 atgatcgagc gttgccattg cggactcaaa tattttcccc gtgtatgatt caacgctgtc
    14221 atcttcattg acgttgcgat taacataggg agtgccattc gccagacttt gtgccagatg
    14281 aagtttaaaa tcaggaaaac cgcgcgtgtc catgctgcga cagaaacgat tcaccgtcgg
    14341 ttcgctaaca ttggcttcca gcgccagagc agcaatactc gaatggatcg cgttatcggg
    14401 cgaagccaga atgacctcgg caactttgcg ctctgatttg ctcaaatgtt ccagctgaga
    14461 ctggattttt tccagcatat tcatgatgta aagagactca cgggtaatga cgatttccgc
    14521 actgaaagaa atcgaaatgc agttttgtca gatattacgc ctgtgtgccg tgttaatgac
    14581 aaaagcagat aaaaaagttg ttattttttt cataacatga tcagtgtcag atttttaccg
    14641 aatggaaaac gatgattttt ttatcagttt tgccgcactt tgcgcgcttt tcccgtaatc
    14701 gcacgggtgg ataagcgttt acagttttcg caagctcgta aaagcagtac agtgcaccgt
    14761 aagaaaatta caagtatacc ctggcttaag taccgggtta gttaacttaa ggagaatgac
    14821 atggcggtaa cgcaaacagc ccaggcctgt gacctggtca ttttcggcgc gaaaggcgac
    14881 cttgcgcgtc gtaaattgct gccttccctg tatcaactgg aaaaagtcgg tcagctcaac
    14941 ccggacaccc ggattatcgg cgtagggcgt gctgactggg ataaagcggc atataccaaa
    15001 gttgtccgcg aggcgctcga aactttcatg aaagaaacca ttgatgaagg tttatgggac
    15061 accctgagtg cacgtctgga tttttgtaat ctcgatgtca atgacactgc tgcattcaac
    15121 cgtctcggcg cgatgctgga tcaaaaaaat cgtatcacta ttaactactt tgccatgccg
    15181 cccagcactt ttggcgcaat ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg
    15241 gcacgcgtag tcatggagaa accgctgggg acgtcgctgg cgacctcgca ggaaatcaat
    15301 gatcaggttg gcgaatactt cgaggagtgc caggtttacc gtatcgacca ctatcttggt
    15361 aaagaaacgg tgctgaacct gttggcgctg cgttttgcta actccctgtt tgtgaataac
    15421 tgggacaatc gcaccattga tcatgttgag attaccgtgg cagaagaagt ggggatcgaa
    15481 gggcgctggg gctattttga taaagccggt cagatgcgtg acatgattca gaaccacctg
    15541 ctacaagttc tctgcatgat tgcgatgtct ccgccatctg acctgagcgc agacagcatc
    15601 cgcgatgaaa aagtgaaagt actgaagtcc ctgcgccgca tcgaccgctc caacgtacgc
    15661 gaaaaaaccg tacgcgggca atatactgcg ggcttcgccc agggcaaaaa agtgccggga
    15721 tatctggaag aagagggcgc gaacaaaagc agcaatacag aaaccttcgt ggcgatccgc
    15781 gtcgacattg ataactggcg ctgggccggt gtgccattct acctgcgtac tggtaaacgt
    15841 ctgccgacca aatgttctga agtcgtggtc tatttcaaaa cacctgaact gaatctgttt
    15901 aaagagtcgt ggcaggatct gccgcagaat aaactgacta tccgtctgca acctgatgaa
    15961 ggcgtggata tccaggtact gaataaagtt cctggccttg accacaaaca taacctgcaa
    16021 atcaccaagc tggatctgag ctattcagaa acctttaatc agacgcatct ggcggatgcc
    16081 tatgaacgtt tgctgctgga aaccatgcgt ggtattcagg cactgtttgt acgtcgcgac
    16141 gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat
    16201 gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt
    16261 acccgtgatg gtcgttcctg gaatgagttt gagtaatatc agcgcttatc ctttatggtt
    16321 attttaccgg taacatgatc ttgcacagat tgtagaacaa tttttacact ttcaggcctc
    16381 gtgcggattc acccacgagg ctttttttat tacactgact gaaacgtttt tgccctatga
    16441 gctccggtta caggcgtttc actcataaat cctctgaatg aaacgcgttg tgaatcatcc
    16501 tgctctgaca actcaatttc aggagccttt atgaatccac aattgttacg cgtaacaaat
    16561 cgaatcattg aacgttcgcg cgagactcgc tctgcttatc tcgcccggat agaacaagcg
    16621 aaaacgtcga ccgttcatcg ttcgcagttg gcatgcggta acctggcaca cggtttcgct
    16681 gcctgccagc cagaagacaa agcctctttg aaaagcatgt tgcgtaacaa tatcgccatc
    16741 atcacctcct ataacgacat gctctccgcg caccagcctt atgaacacta tccagaaatc
    16801 attcgtaaag ccctgcatga agcgaatgcg gttggtcagg ttgcgggcgg tgttccggcg
    16861 atgtgtgatg gtgtcaccca ggggcaggat ggaatggaat tgtcgctgct aagccgcgaa
    16921 gtgatagcga tgtctgcggc ggtggggctg tcccataaca tgtttgatgg tgctctgttc
    16981 ctcggtgtgt gcgacaagat tgtcccgggt ctgacgatgg cagccctgtc gtttggtcat
    17041 ttgcctgcgg tgtttgtgcc gtctggaccg atggcaagcg gtttgccaaa taaagaaaaa
    17101 gtgcgtattc gccagcttta tgccgaaggt aaagtggacc gcat
//
LOCUS       DBKELA010000088        16387 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.088, whole genome shotgun sequence.
ACCESSION   DBKELA010000088 DBKELA010000000
VERSION     DBKELA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.088"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="ACUQR0_002965"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="ACUQR0_002965"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HHV9109914.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="ACUQR0_002966"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="ACUQR0_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HHV9109915.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2295..2438)
                     /gene="yqgG"
                     /locus_tag="ACUQR0_002967"
     CDS             complement(2295..2438)
                     /gene="yqgG"
                     /locus_tag="ACUQR0_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HHV9109916.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2549..2800)
                     /gene="yqgD"
                     /locus_tag="ACUQR0_002968"
     CDS             complement(2549..2800)
                     /gene="yqgD"
                     /locus_tag="ACUQR0_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HHV9109917.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="ACUQR0_002969"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="ACUQR0_002969"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HHV9109918.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="ACUQR0_002970"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="ACUQR0_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HHV9109919.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="ACUQR0_002971"
     CDS             5918..6415
                     /locus_tag="ACUQR0_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HHV9109920.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="ACUQR0_002972"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="ACUQR0_002972"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HHV9109921.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="ACUQR0_002973"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="ACUQR0_002973"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HHV9109922.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="ACUQR0_002974"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="ACUQR0_002974"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HHV9109923.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="ACUQR0_002975"
     CDS             9100..9663
                     /locus_tag="ACUQR0_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HHV9109924.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="ACUQR0_002976"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="ACUQR0_002976"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HHV9109925.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="ACUQR0_002977"
     CDS             complement(10255..11235)
                     /locus_tag="ACUQR0_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HHV9109926.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="ACUQR0_002978"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="ACUQR0_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HHV9109927.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="ACUQR0_002979"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="ACUQR0_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HHV9109928.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /gene="yggU"
                     /locus_tag="ACUQR0_002980"
     CDS             12538..12828
                     /gene="yggU"
                     /locus_tag="ACUQR0_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HHV9109929.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="ACUQR0_002981"
     CDS             12836..13429
                     /locus_tag="ACUQR0_002981"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HHV9109930.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="ACUQR0_002982"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="ACUQR0_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HHV9109931.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="ACUQR0_002983"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="ACUQR0_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16383
                     /locus_tag="ACUQR0_002984"
     CDS             15880..16383
                     /locus_tag="ACUQR0_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HHV9109932.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4058 a         4220 c         4126 g         3983 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggaggccagt
    16381 taatgga
//
LOCUS       DBKELA010000089        16247 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.089, whole genome shotgun sequence.
ACCESSION   DBKELA010000089 DBKELA010000000
VERSION     DBKELA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.089"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..899)
                     /locus_tag="ACUQR0_002985"
                     /pseudo
     CDS             complement(1..899)
                     /locus_tag="ACUQR0_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            1194..1868
                     /locus_tag="ACUQR0_002986"
     CDS             1194..1868
                     /locus_tag="ACUQR0_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHV9109933.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(1907..3568)
                     /locus_tag="ACUQR0_002987"
     CDS             complement(1907..3568)
                     /locus_tag="ACUQR0_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HHV9109934.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(3764..4192)
                     /gene="ibpB"
                     /locus_tag="ACUQR0_002988"
     CDS             complement(3764..4192)
                     /gene="ibpB"
                     /locus_tag="ACUQR0_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HHV9109935.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(4304..4717)
                     /gene="ibpA"
                     /locus_tag="ACUQR0_002989"
     CDS             complement(4304..4717)
                     /gene="ibpA"
                     /locus_tag="ACUQR0_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HHV9109936.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(4711..4783)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            5022..5354
                     /locus_tag="ACUQR0_002990"
     CDS             5022..5354
                     /locus_tag="ACUQR0_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HHV9109937.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(5356..6576)
                     /locus_tag="ACUQR0_002991"
     CDS             complement(5356..6576)
                     /locus_tag="ACUQR0_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HHV9109938.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            6677..7777
                     /gene="cbrA"
                     /locus_tag="ACUQR0_002992"
     CDS             6677..7777
                     /gene="cbrA"
                     /locus_tag="ACUQR0_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HHV9109939.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(7738..9075)
                     /locus_tag="ACUQR0_002993"
     CDS             complement(7738..9075)
                     /locus_tag="ACUQR0_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9109940.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(9150..10298)
                     /gene="dgoD"
                     /locus_tag="ACUQR0_002994"
                     /pseudo
     CDS             complement(9150..10298)
                     /gene="dgoD"
                     /locus_tag="ACUQR0_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(10295..10912)
                     /gene="dgoA"
                     /locus_tag="ACUQR0_002995"
     CDS             complement(10295..10912)
                     /gene="dgoA"
                     /locus_tag="ACUQR0_002995"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HHV9109941.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(10896..11774)
                     /gene="dgoK"
                     /locus_tag="ACUQR0_002996"
     CDS             complement(10896..11774)
                     /gene="dgoK"
                     /locus_tag="ACUQR0_002996"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HHV9109942.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(11771..12460)
                     /gene="dgoR"
                     /locus_tag="ACUQR0_002997"
     CDS             complement(11771..12460)
                     /gene="dgoR"
                     /locus_tag="ACUQR0_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HHV9109943.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            12738..13394
                     /gene="yidX"
                     /locus_tag="ACUQR0_002998"
     CDS             12738..13394
                     /gene="yidX"
                     /locus_tag="ACUQR0_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HHV9109944.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13440..14252)
                     /gene="yidA"
                     /locus_tag="ACUQR0_002999"
     CDS             complement(13440..14252)
                     /gene="yidA"
                     /locus_tag="ACUQR0_002999"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HHV9109945.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14366..14764)
                     /locus_tag="ACUQR0_003000"
     CDS             complement(14366..14764)
                     /locus_tag="ACUQR0_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HHV9109946.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(15004..>16247)
                     /locus_tag="ACUQR0_003001"
     CDS             complement(15004..>16247)
                     /locus_tag="ACUQR0_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HHV9109947.1"
                     /translation="TRRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQG
                     RNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSI
                     IIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEA
                     MDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKEL
                     IYQPTLTEADLSDEQTVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVR
                     THGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDW
                     LVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGD
                     AVEPRRAFIEENALKAANIDI"
BASE COUNT         4070 a         4342 c         4083 g         3752 t
ORIGIN      
        1 cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc aaagattttt gagttaccgt
       61 gcagggcaaa accgccttcc gggaacaacg atttcagtgg ctcggcgctc agactgaact
      121 cttgcagatg ctgcgcccag tacatctgaa tgccgccttc aacagctgcc ggaccaaaga
      181 taaacggtcc gtagatgaag tggtgtaaac cggttgggat cagaatacgt tcgaggaagg
      241 tgtaaaccca caccccaagc gcacccgccg aacgcaggaa cgcttgcaga gattcaatcc
      301 ccatttgtac ttttggccag ccgagcaatg tcagccaggc acaggggatc atcaccagga
      361 aggcgataat caccacataa gacgtcccct ggaaaatgcc gagaaaaacc ggcagttttt
      421 tatcgaacag acggttatgc agcgccgtca caatgccgga aatgataatt gcgccgataa
      481 tgctggtatc gagggtttta atcccggcca tcattgtcag accgctacct gccaccgcgt
      541 cctgagtgaa atcgacgccg aagtagcttc cccaggtcat tcccatcgca ttgatgaaat
      601 agttccaggt caggaaactc accatcaccg ccagacaagc acgtccctgc gcttgcttag
      661 caaggccaat gggtaaaccg acagcaaaaa tcagcggcat attacggaat accgtccaac
      721 cgccctcttc aataatgtgt acgatttgcg cgaataaact gttcggatcg gtcagtgatt
      781 ccccgacaaa acatcgggtt ttgcagcaag atggcaagac ccaccacaat cccggcgaag
      841 ggaaacagca gcactggcgt gaacatcgcg ccgccaaagc gttgaatttg actgagcatt
      901 tccacatcct cttttctcaa ttctgtaagt aggaagcctg attggttatg cggcagacct
      961 ggagcgagga taggaagatg cgatcggcag aacatgacga cccaatggga ttcgtgatcg
     1021 ctttcatgct tttcgttggg cggtagagat ctacgttttc gcgaaaaagt agacatctgt
     1081 gagcacaggt acacagcatc gaacacatct ttaaaaaaaa gatttttttc aatcgattaa
     1141 gcagaacttg tgggcgcatt acccgtgctt gcaggcaaaa aagagagcta gagatgatct
     1201 acaaaagcat tgcggagcgg ttaagaattc gacttaattc cgcggatttc acgctaaaca
     1261 gccttcttcc cggtgaaaaa aagctggcgg aagagtttgc ggtatcgcgg atgactatcc
     1321 gtaaagccat tgacctgctg gtagcgtggg ggctggtggt ccgccgccac ggcagcggca
     1381 cttacctggt gcgcaaagat gtgctgcatc aaaccgccag cctgaccgga ctggtggagg
     1441 tgttaaaacg gcagggaaaa acggtcacca gccaggtgct gatttttgaa atcatgcctg
     1501 cgcctccggc cattgccagc cagttacgga ttcaaatcaa cgagcagatc tacttctccc
     1561 gtcgcgttcg ttttgtggaa gggaaaccgc taatgctgga agacagctat atgccggtaa
     1621 aactgttccg taacctttcg ctgcaacatc tggaagggtc gaagtttgaa tatattgaac
     1681 aagagtgcgg gattttgatt ggcggtaatt atgaaagcct gatgccggtg ctcgccgata
     1741 gactgctggc gcggcaaatg aaggtagcgg aacacacgcc actgctgcgg atcacctcgt
     1801 tgtcatatag cgagagcggg gagtttttga attattcagt gatgttcaga aatgccagcg
     1861 aatactaggt ggagtaccat ttacggcgac tccacccgga aaaaagttaa ccgatactcc
     1921 agaagagcac cgccagtaat tggggggtga taatgcgcag gaacatcact aacggataga
     1981 cagtggcgta agagagcgcc gccgcaccac tggttggatg aagattatta gcaaacgcca
     2041 gcgccggagg atcggtcatg gaacctgcca gcatcccgca catggtcagg taattcattt
     2101 tggctaacat ccgcgccaga atgccaacag taatcagcgg aacggcggtg atcagggcac
     2161 cataaccaat ccagcttagc ccttcgccat tgaccagggt attcacaaaa tccccaccag
     2221 atttcagacc aacgaccgag aggaacagca cgatccccag ctcccgcagc gcgaggttgg
     2281 cacttggcgg cataaaccag tacagcttgc cgatactgcc gatacgcccg aggatcaacg
     2341 ccataatcag cggaccgccc gccagcccca gtttcaacgc ggccgggaat cctggcacaa
     2401 agacgggaat agaacctaac aatacgccaa gcccgatgcc aataaacact ggcagcatct
     2461 gaacctgttg cagtttttgc tgcgcattcc ccagcacatt ggcaacggca tcaattgcgg
     2521 acggacgccc caccaggttg aggatatcgc cgaactgcag gctgatatcg ccactggcga
     2581 ccagttcgac cccggcacgg ttcaggcgcg agataacaac gtcatagcgt tctttaaagt
     2641 gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt gaccaccaca cgctcgacgc
     2701 gcaaatcagt gcctttcgta gacagcgagg tatcgacctc ctgaccaatc accagttgcg
     2761 cattatgtaa atccgctggc tgccccacca gatgcagcaa atcgcccagt tggataatgg
     2821 tatctggcga aggtactttt agggtttctt ctcgtttcag acgcgagcag ataattttgt
     2881 cgccgttgag aatcggtaca tctttaatgg ctaaatcatg caggttaggg ttctcaacgc
     2941 gaatattgat agtcttgatc aacgcgccgc cattggtgcg tgaagactcg tgctgctgag
     3001 cttctgtctc gacattgacg cggaaaataa cccgcaacat ccacatggtg aacaaaatcc
     3061 cgcaaatgcc gaatggatac gccatcgcgt aactcatccc catctgatcg accatttcca
     3121 ttggtgtacc caggtcgcgc aagatctgct gccctgcccc cagcgctggc gtattggtta
     3181 ccgcaccgga gaaaatcccc agcactaccg gcagtggaat atcaaacagt ttatgcagga
     3241 tggcggtaac cagaccaccg atgatgacga tcagaacagc aaacaggttg aggcgtaatc
     3301 cggagacgcg caatgaggca aagaagcccg gccctacctg aatcccgatg gtataaacga
     3361 acaggatcag gccaaactcc tgaataacat gcagcatatc gctacttaat gtcatccccg
     3421 cctgagaaac aaaatggccg acgatgatcc caccaaacag cacgccgcca atacctaatc
     3481 ctatgccgcg aaatttgacg ttgccgataa acaaaccgac gactgccacc aaagccagaa
     3541 tactgaccgt taatgctata tcactcatcg tccatttctc ttgagataac attttaattg
     3601 ctacggatta tggctgaatc ggtgaagcag atttgcctta tagcgcacat tatcacggga
     3661 aagaacgtgc cgaaatatct taaacagtcg aaatttaacg cattgttgaa gaagaaagac
     3721 aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc tagttagcta tttaacgcgg
     3781 gacgttcgct gatagcgata cgctgcgctg cgatgggttc aggctcatta cgaattaaat
     3841 caatatgcag taaaccgttt acgaaggttg cgccagagac ttccatattt tcagccagcg
     3901 taaaactcag gctaaatggc tgattcataa gcccttgatg cagccatttt ttctcttctt
     3961 ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc ttccagttga atctctaaat
     4021 cttcctgacg gaaacctgcc agcgcaaggg taatgcggta gtggttatcg tcgcttttct
     4081 caatgttgta cggcgggaag ctctggcttt caccggcgtt ttgcagtgcg ttggccagtt
     4141 tgtcaaaacc gatccattga cgcatcagtg gggataaatc gaagttacgc atagtcattt
     4201 ctccttctaa gaagcgagta agtacctgca aatccgaaga ttcgcatatg ctccctgacg
     4261 gcgagcatgg agatgtcagg ccgcgccagg cggccttagg gaattagttg atttcgatac
     4321 ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc gatatacagc aaaccattta
     4381 ccaggttagc cccacgaaca tgaatgttct cagctaactg gaatttgcgt tcaaagttgc
     4441 gttcagcgat gccctggtac agataggtgc gctctttttg ttcgtcggcg tgagcacctt
     4501 tcaccaccag cagattatcc tgggcggtaa tttccagttc gctctcagca aaaccagcca
     4561 cagcgatagc aatgcggtaa tggttttcgt ctaccagttc aacgttatac ggagggtagc
     4621 cgccattact ctggctctgg ttgttttcta agtggttaaa caaacggtca aatccaatag
     4681 cagaacggta aagcggggat aaatcaaagt tacgcataat caatagctcc tgaaatcagc
     4741 gagaatgtaa gaccttccac aatggacagg tcaggtagcc agaacaccca tcaggcgcgt
     4801 cctcatcgac tacgatgtaa aatgggtctg gaaatgaact tttcaagcct tatcagcgga
     4861 cttttttttg cagtttatgg tctattgcat agactgaggg ggcagcacga aacgttaaag
     4921 tgcgatagcc gccacagtgc gacgtaatga tgatgttatt ttcagcaagg gatcgctata
     4981 actcatcagg ccaacaccca cgatcaacag atgaatttat tatgataaga aatgtgttgt
     5041 tagcgttcat gatatgcagc ggaatgacat tactcggagg atgctccagc gtgatgtccc
     5101 ataccggggg gaaagaagga acgtatcccg gcacccgcgc cagcgccacc atgattggcg
     5161 acgatgaaac caactggggc actaagtcgc tggcgattct cgatatgccg tttaccgctg
     5221 taatggacac gcttttactg ccgtgggatg tgttccgcaa agacagctcg gtgcgctcgc
     5281 gcgttgaaaa aagcgaagcc aacgcgcaag ccaccaatgc ggttattcca ccggccagaa
     5341 tgcctgataa ctgatttagc gatcagtttc ggtgatccac agctggccac cttccatcca
     5401 cgccagccat tgaccatccg gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg
     5461 gttttcggtt aaatactcaa cctcgccgct ttgcgcatgg gcacaaacaa ttcgattatc
     5521 cagcacaaag cccaaccatt ctcctgacgg atgccagtta aatgcagact gaatatccgt
     5581 tttgttatgg gttaacttgc gcggttcgcc gccctgtggc gagataagcc acagttgcac
     5641 aatgccgtta tcatcacgca ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac
     5701 ccagtggcgc gggacgttga ctaaccccgg ataagcccga tgatgggtaa aggttaaacg
     5761 tcgctgcacg acgccacgcg gtggcgcggg tagggttgtt tccgttccac ttaacggcgc
     5821 atcacctgcc gatttccagc tagcttcatc ttgcggtaac tcaacgataa atagctccgg
     5881 cactttctcg ccctttggcg aaagtgtgtc gccaataaac gccagcgcgt gatttcctac
     5941 ccatccttct tcataagcac gattgatttc atcactgcca ggctgcggcg tgggcgtggt
     6001 tttactcacc agcacacacc agtggctacc gctgtattca cgcggatgtt gcttttgtac
     6061 gttgaccggg ccaaacggtg cagcaacccc gacgtttcgc aaatccagcg ccggatcgag
     6121 ttgatgcatt acatggtcgt tataggtaaa gctccccctt tcaccgttcg ggctaaagac
     6181 atgcacatgg ctgccaccgc gcagcgctcc tggcgtatac ggcgcggtaa tatccattgc
     6241 atcgagattg ctcaccttgc cgccttcagc aatgactccg cgtcgatgat ggaagtcgta
     6301 atgccatgtt tcatcaggat tttcagggcc gtgaataaag acatatttct ctgacttcgg
     6361 atgaacggtc accacgccga cgtgtgcgcc ctgtgacgcg cgatagataa cctcgacctc
     6421 gcctgtatgg atattcacac gctcaatggt ctcgccggta aacgacgccc cagaaggacg
     6481 cacgtcaaat accagccact ggctgtcggg cgtccaggta ttggtattgg tattggtgag
     6541 caggtgatta cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggt
     6601 tcccgccatc atacttcaca aggacttcac tttcagacaa tagtcttttg ccatcgtgat
     6661 gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg
     6721 gtcggcgttg gcacgaaagt tagccggcaa aatgcaggtg atcgcgctgg ataaaaagca
     6781 ccagtgcggt actgaaggtt tcagcaaacc ttgcggcggt ctgctggcac cggacgcgca
     6841 gcgatctttt attcgcgatg gactgacgct tcctgtcgat gtgatcgcca atccacagat
     6901 tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg cgtaactacc agcgaagcta
     6961 tatcaatatt aatcgccatg ctttcgactt gtggatgaaa tcgctgatcc ccgccagcgt
     7021 tgaggtttat cacgatagcc tgtgccgaaa aatctggcgt gaggatgata aatggcatgt
     7081 catttttcgt gcagacggct gggagcaaca tattaccgcc cgctatctgg tcggtgccga
     7141 tggcgcaaac tcgatggtgc ggcgacatct ctacccggat catcaaatcc gtaaatatgt
     7201 cgctatccag cagtggttcg cagagaaaca tccggtgccg ttctactcct gcatctttga
     7261 taatgcgata actgactgtt actcatggag tatcagcaaa gatggttatt ttatctttgg
     7321 cggtgcctat ccaatgaaag acggtcagac gcgtttcacg acgctgaaag agaaaatgag
     7381 cgcctttcag ttccagtttg gcaaagcggt gaaaagtgag aaatgcacgg tgttgtttcc
     7441 ctcacgctgg caggattttg tctgtggtaa ggacaacgcc tttctgattg gtgaagcggc
     7501 gggatttatc agcgccagct cgctggaggg gattagctat gcgctggata gcgcagagat
     7561 tctgcgtgcg gtgttcctga aacagccgga gaagagcaac gccgcttact ggcgcgccac
     7621 acacaaactg cgtttaaaac tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc
     7681 ggctttaaga aagtggatta tgcgcagtgg tgtggcgcat attccacagt tgaaagatta
     7741 tccaacgcgc ttcacatcac ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac
     7801 ggcggagata taaaccagtg caggcgcgaa accgtaaccc tgcgccaggt accccaccac
     7861 cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta aacacgccgc cggttaaacc
     7921 aatcaggcgc atcggtgcca gagaagagac cagcgaccag gtaatcgaag caaaaccgtt
     7981 gccgaagaat gccagcgcca tcaggcacat aatcatcatc ggatcgttgg tgtagttagc
     8041 gcccataatg caggtggaga tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc
     8101 cagtgaaaag cccttacgta ccagcagatc cgcgacccag ccagagagca ggactccgac
     8161 aaacgccgcg aggaatggca ccgtggtcat aaagcctgct ttcagcgccg tgattccttt
     8221 ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac cacagtgcag aagccaccgc
     8281 aaattgccca agataaacgc cgatcagttt acgatggaac accagtttcc agtctttggc
     8341 tgttaacggc tgacgcgcct ctttcttcac cggcgcatca ccatccacca gaccgccgcc
     8401 atcacgaatg taatccagtt cagctttgct gatacctttg gtcaggcgcg gcggctgata
     8461 aaccttaaac caaatcagcg accagataat gccgatacca ccagtgacaa tgaacaccca
     8521 gtgccagctc aacatctcct gaatccagat cagcagcgga gtcaaaaacg ccagaccgac
     8581 aaactgacca gacgtataaa aaccaacggc ggaagcgcgt tcatgttccg ggaaccagct
     8641 ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg aaaataccgg ttatcgcacg
     8701 cagaccaatt aatgacatta atcccgtggc aaagccttgg aataaagtca ccactgacca
     8761 accaaatatc gcaataaaat aagtcacgcg agaacctacg cgatctaaaa accaaccgcc
     8821 ggggatctgg catagcgtat aaagccaggc gaaggccgaa aatacatagc ccatttccgc
     8881 tttcgtaatg ccgaactctt cctgaatatg ggcggaagcc acggccaagt tagcgcggtc
     8941 aacataacaa atgaccaccg taataaagat catcaccagc gtcagataac gccgacgccc
     9001 cggctttgct gcattaacgg gaatatccat agcgagcttt ctccagattt tgggcatagc
     9061 gaagccgctc accatgccct gtaatttaca gagggttata tttttgtatt gctgttttag
     9121 tgcccgatga ggggcttacg tggcaggaat taccactctg ctacgctgtt atcttcatga
     9181 cgccagagcg gattacgcca gtccggggca tttttactga actcaataac tttagcttcg
     9241 tcgatttcca cacctaagcc cggtttcgtt aacggtttaa agaagccgcc aaccatgctg
     9301 aagtcttctt tgtttttcac aaagtcgagt aactccgcgc ctttgttgta atgaatgccc
     9361 atactttgtt actgaagtac cgcgttatag gaaacaaagt cgatatgcag gcaagccgcc
     9421 agtgcaatcg gtccgaacgg acagtgcggc gcaagggtca catcataggc ttctgccatt
     9481 ccagcaattt tgtagcattc ggtaataccg cctgcatggg agagatccgg ttgcagaatc
     9541 gaaataccgc ctgcctccag cacgcgttta aaatcgaaac gtgagaacat gcgctcaccc
     9601 gccgccagtg gaatatgcgt ttgtgccgcc aatttcgggt agtattcggc ttgttccgcc
     9661 agcaccggct cttcaataaa cagcgggcga tacggctcca gttctttaat cagcactttc
     9721 gccattggcg cgctgacgcg accgtggaaa tcaagaccaa actcaatctg attgccaaaa
     9781 gcttcacgaa tttgtgccac ggtgttgact gccgcatcta ccgcgcggga gttatcaatt
     9841 agccccagtt cttcacaacc gttcagtttg aaggtatcga agccgatttc gcgcagggtt
     9901 ttaatgccgt cgataacatc cgccggacga tcgccgccga cccaactgta ggctttaatt
     9961 ttgtcgcgaa ccaggccgcc catcagttgc cagactggcg cattcagcac tttgcctttg
    10021 atatcccata acgcctggtc gatcccggcg atggcgctca tcaggattgg accgccacga
    10081 taaaatccgg cgcgatacat cacttgccat aagtcgttga tgcgcgaagg atcctgacca
    10141 atcaaatagt cacccagctc gtgaactgcc gcttccaccg tacgggcgcg gccttcaatc
    10201 acgggctcgc cccaaccgac cacgccttca tcggtttcaa ttttcaggaa catccagcgg
    10261 ggaggtaaac gatacgtggt aattttggtg attttcattg cactgcctct cgatacgcct
    10321 taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga ctgcccggcg cgataaagat
    10381 cgctgcctaa gcccgcccca acacaacctg cgtctatcca ctgcgccagg ttttctggcg
    10441 tcacgccgcc aacggcaaag actgcgatgt ccggtggtaa taccgctttt aacgctttga
    10501 tgtattgcgg accaaaagcc gatgacggaa atattttcag cgcctgcgcg cccgcttcga
    10561 gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca aacggtcatg ccgtagccca
    10621 ccgcgcggcg gatcacttca ctatggatat tgggcgtaac gatgagctga cagcccatcc
    10681 tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt
    10741 cgccatacgc atcaacgatg gcgggaatgc tctgctccca ttgtggggaa ttcagcggga
    10801 tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg
    10861 gcgtaatgcc gcgcaaaatg gcgatcagcg ggagtttagt ttgccactgc atgagcgatg
    10921 ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg ccgccacgtc gcaacccatc
    10981 gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg
    11041 tgttgatggg tcacatagtc gcgcatactg gcaacctctg cgccaatcaa caaaccagag
    11101 agaaattcgc tgacctgttc gcggggaagt gttcccagca catgcgaggc gcgaacttca
    11161 aaaagctgcg gcaatatggc gggcgcatta aggccgcgct caaggccagc cgcgaaggca
    11221 tcggcagagt tttcctgcgg cggcaaacct gcgccaatca atgagtgatt taacagtaaa
    11281 tgatgtaatt caccggtcat cacggtgcga aaatcgttga tttgctggct atcggcctgc
    11341 acccatttgc aatgcgtacc gggcataaca taaagagagg aaggagccag agctcgcgcg
    11401 ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt tatcgtcatg agagacgcat
    11461 aatccgggaa taatccagat attgtcgcca acagacgtta attgttcgcc aatagacgaa
    11521 aaacgggcag gaacagataa atacggagca actttccagc cgacattgct gccgaccatt
    11581 cctgccatca ccactggcgt attttcttca cgccagtcgg tcgtgacttc tgctaacacc
    11641 acagccggag attttccgtt caggcgcgtg acgcctgctt ctgattgcct gctctccagg
    11701 cagtggtcgc cctgataaag ccaggcgcgc agattggtcg atccccagtc aattgcgatg
    11761 tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga gctggcaatc atggtaagcg
    11821 ccgcctgctc tgccgcatcg ccgtcctgat gccgtatcgc atcgaacagc gccttatgtt
    11881 cctggagcgt ttgcggcatg ttggcctcat cgcccatcca ggttcgttca aaaaccgccc
    11941 gctgcagcga actgatcgca atgctaagtt gctgtaacac cgggttatgc accgactgca
    12001 gcaccgcctc gtggtagcga atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg
    12061 caatcatctc gttcagcgcc gattcaatct gcgccagatc gctggaggtc gcgcgctctg
    12121 ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac ttcgctcatg gcactgataa
    12181 gccgtgggtc gtagtcgttt tccagcaccc attgcagtac gtcagtgtcg aggtaattcc
    12241 actggttacg cggtgccaca aacgccccgc gataacgttt catttcaatc agccgcttcg
    12301 ccatcagcga acggaacacc tcacggatga tgttgcgcga ggttgcaaac tcttcacaga
    12361 gctccgcctc agccggaagt ggcgagccag gtacgtattt gccgtgaacg atctgtttac
    12421 ccagcgtaat gacaatgcga tcggttttat tgagagtcat ggagagtcct tgtgctctgt
    12481 atgttcttct ctactttacc ccgatcgatg cataacgcgg caactttgta gtaccagcgt
    12541 gatgacgttc gcgtttgccg cgcgcgtaat gtagtacaaa cttatattgt tatgctacaa
    12601 tttagatcaa aaaaagaaca atacataaaa aaatgacatg cgtcgggcag aaatctgaaa
    12661 agggatatca ggcgctaaac aggagggaaa gaagagtatg ctttcaacgg cttagctact
    12721 cgtttaaagg attaatcatg aagttgaatt ttaagggatt ttttaaggct gccggtttat
    12781 tcccactggc gctgatgctt tcaggctgta tctcgtatgc tctggtttcc cataccgcaa
    12841 agggtagttc aggaaagtat caatcgcagt cagacaccat cactgggcta tcgcaggcaa
    12901 aagatagtaa tggaacaaaa ggctatgttt ttgtagggga atcgctggat taccttatca
    12961 ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc agcacttaac cggcacaata
    13021 ttcaggttgc cgatgacgca agatttgttt taaatgcggg gaaaaagaaa tttaccggca
    13081 caatatcgct ttactactac tggaataacg aagaagaaaa ggcactggca acgcattatg
    13141 gttttgcctg tggtgttcaa cactgtacca ggtcactgga aaacctaaaa ggcacaatcc
    13201 atgagaaaaa taaaaacatg gattactcaa aggtgatggc gttctaccat ccatttaaag
    13261 tgcgatttta tgaatactat tcacccagag gcattccgga tggtgtttcc gcagcattac
    13321 tgccagtgac tgttacactg gacatcatta ctgcaccgct gcaatttctg gttgtatatg
    13381 cagtaaacca ataatcagta agcgggcaaa cgcgtttatg ctgtttgccc gcttactgat
    13441 taattcagca catacttctc aatagcaaac gccacgccat cttcaaggtt ggattttgtg
    13501 acaaagttcg ccacttcttt caccgaagga atagcgttat ccatcgccac accgacgcct
    13561 gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa tcgccatgat ttcttccggt
    13621 ttaataccta acacgtcggc cagtgatttc acacccgtac ctttgttaac gcgtttatcg
    13681 aggatttcga ggaagtacgg cgcacttttc agcacggtat atttctcttt cacttcctgc
    13741 ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat caatcatcat cactttcagg
    13801 aactgggtat tggggtccat tttctccgct tcgcagaaca ccagcggaat tgtggcaacg
    13861 aaggattcat gcaccgtgta gtagctgata tcgcggttag cggtgtacag cgtggtgcgg
    13921 tccagggcgt ggaaatgaga accgacttcg cgagagagtt tttccaggaa acgatagtcg
    13981 tcatagctga gagcggtttg cgccacggtg ctaccatcag cggccttctg taccagcgcg
    14041 ccgttataag taatgcagta gtcgcccggc tgttccatat gcagctcttt caggtagttg
    14101 tgcacacctg catacggacg acccgtcgtt agcacgacat tcacgccacg ggcgcgagct
    14161 gcggcaatcg catttttaac ggcgggtgaa atggtgtgat cgggcagcag aagggtgcca
    14221 tccatatcga tagcaatgag tttgatagcc atgagttccc aaggtagatt agttcctgac
    14281 ccatgctaac gcgattccgc tcaaaaatca gtacaacacc cgagggaaaa ggggatgcaa
    14341 cgcgggttgc atgctcccca tttgcttagc ggaagagttt ccctttcagc agttccatgc
    14401 ctgcggaaag cagatcgtta ttggcttgtg ctgacacttc accttgcggt gagagcgcat
    14461 caataatctt cggcaattgc tctgccagta aactggaagc cgtactggta tccacgccaa
    14521 gtttttgccc gagatcggac accgcatttg tgccgagcgc cgactccagt tgctcgccgc
    14581 taactgattg atttctctgt tgattactca accaggttga gagaatggcc cctaagccgc
    14641 cactttgcag tttttccagc agcacctgaa tgccaccctg ctcctcaacc caacttaaaa
    14701 tcgcctgata tttccccgca tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc
    14761 ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat ctgcgggatg
    14821 ataattgcgg attgcagaaa taaaaagggc ggagatgatc tccgcccttt tcttatagct
    14881 tcttgccgga tgcggcgtga acgccttatc cggcctacaa aatcatgcaa aatcaatata
    14941 ttgcaggagt ctcgtaggac taataagcgt agcgcatcag gcacgctcgc atggttaacg
    15001 ccattaaata tcaatattcg ccgctttcag ggcgttctct tcaataaacg cacgacgcgg
    15061 ttctacggcg tcgcccatca gcgtggtgaa caactggtca gcggcaatcg catccttaac
    15121 ggtaacgcgc agcatacgac ggctttccgg gtccatggtg gtttcccaca gctgttccgg
    15181 gttcatctcg cccagacctt tataacgctg gatggagagg ccacgacggg actctttcac
    15241 cagccagtcc agcgcctgct cgaagctggc taccggctga cgacgctcgc cacgttcgat
    15301 aaacgcatct tcttccagca agccacgcag tttctcaccc agcgtgcaga tacgacgata
    15361 ttcgccaccg gtgataaact cgtgatccag cggatagtca gtatccacac cgtgggtacg
    15421 cacgcgaaca atcggctcga acaggttttg ttcggcattg gtgtggacat caaacttcca
    15481 ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt
    15541 aacggtctgc tcatcagaaa ggtcagcttc cgtcaacgtc ggctgataga taagctcttt
    15601 cagcattgct ttcggataac gacgctccat gcggttgatc attttctgcg tcgcgttgta
    15661 ctcagatacc agtttctcta acgcttcgcc agccaatgcc ggtgcactgg cgttggtgtg
    15721 cagcgttgcg ccgtccagcg cgatagagat ctggtactga tccatcgctt cgtcgtcttt
    15781 aatgtactgt tcctgcttgc ctttcttcac tttgtacagc ggcggctgag cgatgtagac
    15841 gtgaccgcgt tcaacgattt ccggcatctg acgatagaag aaggtcaaca gcagcgtacg
    15901 aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat aacgcagttt
    15961 gtccgggttg tactcgtcac gaccgatacc acagccaagc gcggtgataa gcgtcgccac
    16021 ttcctgagaa gagagcatct tatcgaagcg cgctttctcg acgttaagga ttttaccctt
    16081 cagcggcaga atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga
    16141 gtccccttcc accaggtaca gttcggaaag cgccggatcg cgttcctggc agtctgccag
    16201 tttgcccggc aggccagcta agtcgagcgc acctttacgg cgggtca
//
LOCUS       DBKELA010000090        16098 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.090, whole genome shotgun sequence.
ACCESSION   DBKELA010000090 DBKELA010000000
VERSION     DBKELA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.090"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..588
                     /locus_tag="ACUQR0_003002"
     CDS             <1..588
                     /locus_tag="ACUQR0_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HHV9109948.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACUQR0_003003"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACUQR0_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HHV9109949.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACUQR0_003004"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACUQR0_003004"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HHV9109950.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACUQR0_003005"
     CDS             complement(1511..1978)
                     /locus_tag="ACUQR0_003005"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HHV9109951.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2359..2670
                     /gene="rpsJ"
                     /locus_tag="ACUQR0_003006"
     CDS             2359..2670
                     /gene="rpsJ"
                     /locus_tag="ACUQR0_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HHV9109952.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2703..3332
                     /gene="rplC"
                     /locus_tag="ACUQR0_003007"
     CDS             2703..3332
                     /gene="rplC"
                     /locus_tag="ACUQR0_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HHV9109953.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3343..3948
                     /gene="rplD"
                     /locus_tag="ACUQR0_003008"
     CDS             3343..3948
                     /gene="rplD"
                     /locus_tag="ACUQR0_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HHV9109954.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3945..4247
                     /gene="rplW"
                     /locus_tag="ACUQR0_003009"
     CDS             3945..4247
                     /gene="rplW"
                     /locus_tag="ACUQR0_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HHV9109955.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4265..5086
                     /gene="rplB"
                     /locus_tag="ACUQR0_003010"
     CDS             4265..5086
                     /gene="rplB"
                     /locus_tag="ACUQR0_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HHV9109956.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5103..5381
                     /gene="rpsS"
                     /locus_tag="ACUQR0_003011"
     CDS             5103..5381
                     /gene="rpsS"
                     /locus_tag="ACUQR0_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HHV9109957.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5396..5728
                     /gene="rplV"
                     /locus_tag="ACUQR0_003012"
     CDS             5396..5728
                     /gene="rplV"
                     /locus_tag="ACUQR0_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HHV9109958.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5746..6447
                     /gene="rpsC"
                     /locus_tag="ACUQR0_003013"
     CDS             5746..6447
                     /gene="rpsC"
                     /locus_tag="ACUQR0_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HHV9109959.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6460..6870
                     /gene="rplP"
                     /locus_tag="ACUQR0_003014"
     CDS             6460..6870
                     /gene="rplP"
                     /locus_tag="ACUQR0_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HHV9109960.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6870..7061
                     /gene="rpmC"
                     /locus_tag="ACUQR0_003015"
     CDS             6870..7061
                     /gene="rpmC"
                     /locus_tag="ACUQR0_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HHV9109961.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7061..7315
                     /gene="rpsQ"
                     /locus_tag="ACUQR0_003016"
     CDS             7061..7315
                     /gene="rpsQ"
                     /locus_tag="ACUQR0_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HHV9109962.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7480..7851
                     /gene="rplN"
                     /locus_tag="ACUQR0_003017"
     CDS             7480..7851
                     /gene="rplN"
                     /locus_tag="ACUQR0_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HHV9109963.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7862..8176
                     /gene="rplX"
                     /locus_tag="ACUQR0_003018"
     CDS             7862..8176
                     /gene="rplX"
                     /locus_tag="ACUQR0_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HHV9109964.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8191..8730
                     /gene="rplE"
                     /locus_tag="ACUQR0_003019"
     CDS             8191..8730
                     /gene="rplE"
                     /locus_tag="ACUQR0_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HHV9109965.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8745..9050
                     /gene="rpsN"
                     /locus_tag="ACUQR0_003020"
     CDS             8745..9050
                     /gene="rpsN"
                     /locus_tag="ACUQR0_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HHV9109966.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9084..9476
                     /gene="rpsH"
                     /locus_tag="ACUQR0_003021"
     CDS             9084..9476
                     /gene="rpsH"
                     /locus_tag="ACUQR0_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HHV9109967.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9489..10022
                     /gene="rplF"
                     /locus_tag="ACUQR0_003022"
     CDS             9489..10022
                     /gene="rplF"
                     /locus_tag="ACUQR0_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HHV9109968.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10032..10385
                     /gene="rplR"
                     /locus_tag="ACUQR0_003023"
     CDS             10032..10385
                     /gene="rplR"
                     /locus_tag="ACUQR0_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HHV9109969.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10400..10903
                     /gene="rpsE"
                     /locus_tag="ACUQR0_003024"
     CDS             10400..10903
                     /gene="rpsE"
                     /locus_tag="ACUQR0_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HHV9109970.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10907..11086
                     /gene="rpmD"
                     /locus_tag="ACUQR0_003025"
     CDS             10907..11086
                     /gene="rpmD"
                     /locus_tag="ACUQR0_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HHV9109971.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11090..11524
                     /gene="rplO"
                     /locus_tag="ACUQR0_003026"
     CDS             11090..11524
                     /gene="rplO"
                     /locus_tag="ACUQR0_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HHV9109972.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11532..12863
                     /gene="secY"
                     /locus_tag="ACUQR0_003027"
     CDS             11532..12863
                     /gene="secY"
                     /locus_tag="ACUQR0_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HHV9109973.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12895..13011
                     /gene="rpmJ"
                     /locus_tag="ACUQR0_003028"
     CDS             12895..13011
                     /gene="rpmJ"
                     /locus_tag="ACUQR0_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HHV9109974.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13158..13514
                     /gene="rpsM"
                     /locus_tag="ACUQR0_003029"
     CDS             13158..13514
                     /gene="rpsM"
                     /locus_tag="ACUQR0_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HHV9109975.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13531..13920
                     /gene="rpsK"
                     /locus_tag="ACUQR0_003030"
     CDS             13531..13920
                     /gene="rpsK"
                     /locus_tag="ACUQR0_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HHV9109976.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13954..14574
                     /gene="rpsD"
                     /locus_tag="ACUQR0_003031"
     CDS             13954..14574
                     /gene="rpsD"
                     /locus_tag="ACUQR0_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HHV9109977.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14600..15589
                     /locus_tag="ACUQR0_003032"
     CDS             14600..15589
                     /locus_tag="ACUQR0_003032"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HHV9109978.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15630..16013
                     /gene="rplQ"
                     /locus_tag="ACUQR0_003033"
     CDS             15630..16013
                     /gene="rplQ"
                     /locus_tag="ACUQR0_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HHV9109979.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4142 a         3786 c         4292 g         3878 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt actattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccaatcgt ggtaagaagg
     1681 cccatgaatg ccaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agagcgctga tttgttaacc tgattccggt gaaaaacgct atcttcacaa ccttacaaat
     2101 ttcgtggaat gcagctcgca atctgcgcgt tgcgattaat ttctacccga tgcttgcgtc
     2161 aggctcagat ttgcgtataa tgcgcgggct tgtcgtagtt gacagcaggt tcaatctgaa
     2221 cctcagttag ccgaatttgg ctacctaaca atgctcccaa tcggggagct acgtaagaac
     2281 ggttacactc tcccatcaat cgtaatgggt ctgaggagta atcattttcg tttataaaat
     2341 aattggagct ctggtctcat gcagaaccaa agaatccgta tccgcctgaa agcgtttgat
     2401 catcgtctga tcgatcaagc aaccgcggaa atcgtcgaga ctgccaagcg cactggtgcg
     2461 caggtccgtg gtccgatccc gctgccgaca cgcaaagagc gcttcactgt tctgatctcc
     2521 ccgcacgtca acaaagacgc gcgcgatcag tacgaaatcc gtactcactt gcgtctggtt
     2581 gacatcgttg agccaaccga gaaaaccgtt gatgctctga tgcgtctgga tctggctgcc
     2641 ggtgtagacg tgcagatcag cctgggttaa tcaggtcatt gagcgattga gaggttgaaa
     2701 caatgattgg tttagtcggt aaaaaagtgg gtatgacccg tatcttcaca gaagacggcg
     2761 tttctatccc agtaaccgta atcgaagttg aagcaaaccg cgttactcag gttaaagacc
     2821 tggctaacga tggctaccgt gctattcagg tgaccaccgg tgctaaaaaa gctaaccgtg
     2881 tgaccaagcc tgaagctggc cacttcgcta aagctggcgt agaagctggc cgtggtctgt
     2941 gggaattccg cctggctgaa ggcgaagagt tcactgtagg tcagagcatt agcgttgaac
     3001 tgtttgctga cgttaaaaaa gttgacgtaa ctggcacctc taaaggtaaa ggtttcgcag
     3061 gtaccgttaa gcgctggaac ttccgtaccc aggacgctac tcacggtaac tccttgtctc
     3121 accgcgttcc gggttctatc ggtcagaacc agactccggg caaagtgttc aaaggcaaga
     3181 aaatggcagg tcagatgggt aacgaacgtg taaccgttca gagccttgac gtagtacgcg
     3241 ttgacgctga gcgcaacctg ctgctggtta aaggtgctgt cccgggtgca accggtagcg
     3301 acctgatcgt taaaccagct gtgaaggcgt aaggagatag caatggaatt agtattgaaa
     3361 gacgcgcaga gcgcgctgac tgtttccgaa actaccttcg gtcgtgattt caacgaagcg
     3421 ctggttcacc aggttgttgt tgcttatgca gctggtgctc gtcagggtac tcgtgctcag
     3481 aagactcgtg ctgaagtaac tggttccggt aaaaaaccgt ggcgccagaa aggcaccggc
     3541 cgtgcgcgtt ctggttctat caagagcccg atctggcgtt ctggtggcgt gacctttgct
     3601 gctcgtccgc aggaccacag tcaaaaagtt aacaagaaga tgtaccgcgg cgcgctgaaa
     3661 agcatcctgt ccgaactggt acgtcaggat cgtctgatcg ttgtcgagaa gttctctgta
     3721 gaagcgccga aaactaagct gctggcacag aaactgaaag acatggctct ggaagatgtg
     3781 ctgatcatca ccggtgagct ggacgaaaac ctgttcctgg ctgcgcgcaa cctgcagaag
     3841 gttgacgtac gcgatgcaac tggtatcgac ccggttagcc tgatcgcctt cgacaaagtc
     3901 gtaatgactg ctgatgctgt taagcaagtt gaggagatgc tggcatgatt cgtgaagaac
     3961 gtctgctgaa ggtgctgcgt gcaccgcacg tttctgaaaa agcgtctact gcgatggaaa
     4021 aatccaacac catcgtactc aaagttgcga aagacgcgac caaagcagaa atcaaagctg
     4081 ctgtgcagaa actgtttgaa gtcgaagtcg aagtcgttaa caccctggta gttaaaggga
     4141 aagttaaacg tcacggacag cgtatcggtc gtcgtagcga ctggaaaaaa gcttacgtca
     4201 ccctgaaaga aggccagaat ctggacttcg ttggcggcgc tgagtaagtc ggaggagtaa
     4261 tacaatggca gttgttaaat gtaaaccgac atctccgggt cgtcgccacg tagttaaagt
     4321 ggttaaccct gagctgcaca agggcaaacc ttttgctccg ttgctggaaa aaaacagcaa
     4381 atccggtggt cgtaacaaca atggccgtat caccactcgt catatcggtg gtggccacaa
     4441 gcaggcttac cgtattgttg acttcaaacg caacaaagac ggtatcccgg cagttgttga
     4501 acgtcttgag tacgatccga accgttccgc gaacatcgcg ctggttctgt acaaagacgg
     4561 tgaacgccgt tacatcctgg cccctaaagg cctgaaagct ggcgaccaga ttcagtctgg
     4621 cgttgatgct gcaatcaaac caggtaacac cctgccgatg cgcaacatcc cggttggttc
     4681 tactgttcat aacgtagaaa tgaaaccagg taaaggcggt cagctggcac gttccgctgg
     4741 tacttacgtt cagatcgttg ctcgtgatgg tgcttatgtc accctgcgtc tgcgttctgg
     4801 tgaaatgcgt aaagtagaag cagactgccg tgcaactctg ggcgaagttg gcaatgctga
     4861 gcatatgctg cgcgttctgg gtaaagcagg tgctgcacgc tggcgtggtg ttcgtccgac
     4921 cgttcgcggt accgcgatga acccggtaga ccacccacat ggtggtggtg aaggtcgtaa
     4981 ctttggtaag cacccggtaa ctccgtgggg cgttcagacc aaaggtaaga agacccgcag
     5041 caacaagcgt actgataaat tcatcgtacg tcgccgtagc aaataatttt agaggataag
     5101 ccatgccacg ttctctcaag aaaggtcctt ttattgacct gcacttgctg aagaaggtag
     5161 agaaagcggt ggaaagcgga gacaagaagc ccctgcgcac ttggtcccgt cgttcaacga
     5221 tctttcctaa catgatcggt ttgaccatcg ctgtccataa tggtcgtcag cacgttccgg
     5281 tatttgtaac cgacgaaatg gtcggtcaca aactgggtga attcgcaccg actcgtactt
     5341 atcgcggcca cgctgctgat aaaaaagcga agaagaaata aggtaggagg aagagatgga
     5401 aactattgct aaacatcgcc atgctcgttc ttctgctcag aaggttcgcc ttgttgctga
     5461 cctgattcgc ggtaagaaag tgtcgcaggc tctggatatt ttgacctaca ccaacaagaa
     5521 agcggctgta caggtcaaga aagttctgga atctgccatt gctaacgctg aacacaacga
     5581 tggcgctgac attgacgatc tgaaagttac gaaaattttc gtagacgaag gcccgagcat
     5641 gaagcgcatt atgccgcgtg caaaaggtcg tgcagatcgc atcctgaagc gcaccagcca
     5701 catcactgtg gttgtgtccg atcgctgaga ctctggagac tagcaatggg tcagaaagta
     5761 catcctaatg gtattcgctt gggtattgta aaaccatgga actctacttg gtttgcgaac
     5821 accaaagaat tcgctgacaa cctggacagc gattttaaag tacgtcagta cctgactaag
     5881 gaactggcta aagcgtccgt atctcgtatc gttatcgagc gtccggctaa gagcatccgt
     5941 gtaaccattc acactgctcg cccgggtatc gttatcggta aaaaaggtga agacgtagaa
     6001 aaactgcgta aggtcgtagc ggacatcgct ggcgttcctg cacagatcaa catcgccgaa
     6061 gttcgtaagc ctgaactgga cgcaaaactg gttgctgaca gcatcacttc tcagctggaa
     6121 cgtcgcgtta tgttccgtcg tgctatgaag cgtgctgtac agaacgcaat gcgtctgggc
     6181 gctaaaggta ttaaagttga agttagcggc cgtctgggcg gcgcggaaat cgcacgtacc
     6241 gaatggtacc gcgaaggtcg cgtaccgctg cacactctgc gtgctgacat cgactacaac
     6301 acctctgaag cgcacaccac ttacggtgta atcggcgtta aagtgtggat cttcaaaggc
     6361 gagatcctgg gtggtatggc tgctgttgaa caaccggaaa aaccggctgc tcagcctaaa
     6421 aagcagcagc gtaaaggccg taaataagga gcgtcgctga tgttacaacc aaagcgtaca
     6481 aaattccgta aaatgcacaa aggccgtaac cgcggtctgg cgcagggtac ggatgttagc
     6541 ttcggcagct tcggtctgaa agctgttggc cgtggtcgtc tgactgcccg tcagatcgaa
     6601 gcagcacgtc gtgctatgac ccgtgcagtt aagcgtcaag gtaagatctg gatccgtgtg
     6661 ttcccggaca aaccgatcac tgaaaagccg ctggcagtgc gtatgggtaa aggtaaaggt
     6721 aacgtggagt attgggttgc cttgattcag ccgggtaaag tcctgtatga aatggacggt
     6781 gttccggaag agctggcccg tgaagcattc aagctggcag cagcgaaact gccgattaaa
     6841 accacctttg taactaagac ggtgatgtaa tgaaagcaaa agagctgcgt gagaagagca
     6901 ttgaagagct gaacaccgag ctgctgaacc tgctgcgtga gcagttcaac ctgcgtatgc
     6961 aggctgcaag tggccagctg caacagtctc acctgttgaa gcaagtgcgt cgcgatgtcg
     7021 cacgcgttaa gactttactg aacgagaagg cgggtgcgta atgaccgata aaatccgtac
     7081 tctgcaaggt cgcgttgtta gcgacaaaat ggagaaatcc attgttgttg ctatcgaacg
     7141 ttttgtgaaa cacccgatct acggtaaatt catcaagcgt acgaccaaac tgcacgtaca
     7201 tgacgagaac aacgaatgcg gtatcggtga cgtggttgaa atccgcgaat gccgtccgct
     7261 gtccaagact aaatcctgga cgctggttcg cgttgtagag aaagcggttc tgtaatacag
     7321 tacactctct cgatacgaat aaacggctca gaaatgagcc gtttattttt tctacccata
     7381 tccttgaagc ggtgttataa tgccgcgccc tcgatatggg gatttttaac gacctgattt
     7441 tcgggtctca gtagtagttg acattagcgg agcactaaaa tgatccaaga acagactatg
     7501 ctgaacgtcg ccgacaactc cggtgcacgt cgcgtaatgt gtatcaaggt tctgggtggc
     7561 tcgcaccgtc gctacgcagg cgtaggcgac atcatcaaga tcaccatcaa agaagcaatt
     7621 ccgcgtggta aggtcaaaaa aggtgatgtg ctgaaggcgg tagtggtgcg caccaagaag
     7681 ggtgttcgtc gcccggacgg ttctgtcatt cgcttcgatg gtaatgcttg tgttcttctg
     7741 aacaacaaca gcgagcagcc tatcggtacg cgtatttttg ggccggtaac tcgtgagctt
     7801 cgtagtgaga agttcatgaa aattatctct ctggcaccag aagtactcta aggagcgaat
     7861 catggcagcg aaaatccgtc gtgatgacga agttatcgtg ttaaccggta aagataaagg
     7921 taaacgcggt aaagttaaga atgtcctgtc ttccggcaag gtcattgttg aaggtatcaa
     7981 cctggttaag aaacatcaga agccggttcc ggccctgaac caaccgggtg gcatcgttga
     8041 aaaagaagcc gctattcagg tttccaacgt agcaatcttc aatgcggcaa ccggcaaggc
     8101 tgaccgtgta ggctttagat tcgaagacgg caaaaaagtc cgtttcttca agtctaacag
     8161 cgaaactatc aagtaatttg gagtagtacg atggcgaaac tgcatgatta ctacaaagac
     8221 gaagtagtta aaaaactcat gactgagttt aactacaatt ctgtcatgca agtccctcgg
     8281 gtcgagaaga tcaccctgaa catgggtgtt ggtgaagcga tcgctgacaa aaaactgctg
     8341 gataacgcag cagcagacct ggcagcaatc tccggtcaaa aaccgctgat caccaaagca
     8401 cgcaaatctg ttgcaggctt caaaatccgt cagggctatc cgatcggctg taaagtaact
     8461 ctgcgtggcg aacgcatgtg ggagttcttt gagcgcctga tcactattgc tgtacctcgt
     8521 atccgtgact tccgtggcct gtccgctaag tctttcgacg gtcgtggtaa ctacagcatg
     8581 ggtgtccgtg agcagatcat cttcccagaa atcgactacg ataaagtcga ccgcgttcgt
     8641 ggtttggata ttaccattac cactactgcg aaatctgacg aagaaggccg cgctctgctg
     8701 gctgcctttg acttcccgtt ccgcaagtaa ggtagggtta ctaaatggct aagcaatcaa
     8761 tgaaagcacg cgaagtaaaa cgcgtagctt tagctgataa atacttcgcg aaacgcgctg
     8821 aactgaaagc gatcatctct gatgtgaacg cttccgacga agatcgttgg aacgctgttc
     8881 tcaagctgca gactctgccg cgtgattcca gcccgtctcg tcagcgtaac cgctgccgtc
     8941 aaacaggtcg tccgcatggt ttcctgcgga agttcgggtt gagccgtatt aaggtccgtg
     9001 aagccgctat gcgcggtgaa atcccgggtc tgaaaaaggc tagctggtaa ttgtcaccaa
     9061 ttgaatcacg ggagtaaaga cagatgagca tgcaagatcc gatcgcggat atgctgaccc
     9121 gtatccgtaa cggtcaggcc gcgaacaaag ctgcggtcac catgccttcc tccaagctga
     9181 aagtggcaat cgccaacgtg ctgaaggaag aaggttttat tgaagatttt aaagttgaag
     9241 gcgacaccaa gcctgaactg gaacttactc tgaagtattt ccagggcaaa gctgttgtag
     9301 aaagcattca gcgtgtcagc cgcccaggtc tgcgcatcta taaacgtaaa gatgagctgc
     9361 cgaaagttat ggcgggtctg ggtatcgcag ttgtttctac ctctaaaggt gttatgactg
     9421 atcgtgcagc gcgccaggct ggtcttggtg gcgaaattat ctgctacgta gcctaatcgg
     9481 aggaaaaaat gtctcgtgtt gctaaagcac cggtcgttgt tcctgccggc gttgacgtaa
     9541 aaatcaacgg tcaggttatt acgatcaaag gtaaaaacgg cgagctgact cgtactctca
     9601 acgatgctgt tgaagttaaa catgcagata ataccctgac cttcggtccg cgtgatggtt
     9661 acgcagacgg ttgggcacag gctggtaccg cgcgtgccct gctgaactca atggttatcg
     9721 gtgttaccga aggcttcact aagaagctgc agctggttgg tgtaggttac cgtgcagcgg
     9781 ttaaaggcaa tgtgattaac ctgtctctgg gtttctctca tcctgttgac catcagctgc
     9841 ctgcgggtat cactgctgaa tgtccgactc agactgaaat cgtgctgaaa ggcgctgata
     9901 agcaggtgat cggccaggtt gcagcggatc tgcgcgccta ccgtcgtcct gagccttaca
     9961 aaggcaaggg tgttcgttac gccgacgaag tcgtgcgtac caaagaggct aagaagaagt
    10021 aaggtaacac tatggataag aaatctgctc gtatccgtcg tgcgacccgc gcacgccgca
    10081 agctccagga gctgggcgca actcgcctgg tggtacatcg taccccgcgt cacatttacg
    10141 cacaggtaat tgcaccgaac ggttctgaag ttctggtagc tgcttctact gtagaaaaag
    10201 ctatcgctga acaactgaag tacaccggta acaaagacgc ggctgcagct gtgggtaaag
    10261 ctgtcgctga acgcgctctg gaaaaaggca tcaaagatgt atcctttgac cgttccgggt
    10321 tccaatatca tggtcgtgtc caggcactgg cagatgctgc ccgtgaagct ggccttcagt
    10381 tctaaggtag aggtgtaaga tggctcacat cgaaaaacaa gctggcgaac tgcaggaaaa
    10441 gctgatcgcg gtaaaccgcg tatctaaaac cgttaaaggt ggtcgtattt tctccttcac
    10501 agctctgact gtagttggcg atggtaacgg tcgcgttggt tttggttacg gtaaagcgcg
    10561 tgaagttcca gcagcgatcc agaaagcgat ggaaaaagcc cgtcgcaata tgattaacgt
    10621 cgcgctgaat aacggcactc tacaacaccc tgttaaaggt gttcacacgg gttctcgcgt
    10681 attcatgcag ccggcttccg aaggtaccgg tatcatcgcc ggtggtgcaa tgcgcgccgt
    10741 tctggaagtc gctggggttc ataacgttct ggctaaagca tatggttcca ccaacccgat
    10801 caacgtggtt cgtgcaacta ttgatggcct ggaaaatatg aattctccag aaatggtcgc
    10861 tgccaagcgt ggtaaatccg ttgaagaaat tctggggaaa taaaccatgg caaagactat
    10921 taaaattact caaacccgca gtgcaatcgg tcgtctgccg aaacacaagg caacgctgct
    10981 tggcttgggt ctgcgtcgta ttggtcacac cgtagagcgc gaggatactc ctgctattcg
    11041 cggtatgatc aacgcggttt ccttcatggt taaagttgag gagtaagaga tgcgtttaaa
    11101 tactctgtct ccggccgaag gctccaaaaa ggcgggtaaa cgcctgggtc gtggtatcgg
    11161 ttctggcctc ggtaaaaccg gtggtcgtgg tcacaaaggt cagaagtctc gttctggcgg
    11221 tggcgtacgt cgcggtttcg agggtggtca gatgcctctg taccgtcgtc tgccgaaatt
    11281 cggcttcact tctcgtaaag cagcgattac agccgaagtt cgtctatctg acctggctaa
    11341 agtagaaggc ggtgttgtag acctgaacac gctgaaagcg gctaacatta tcggtatcca
    11401 gatcgagttc gcgaaagtga tcctggctgg cgaagtaacg actccggtaa ctgttcgtgg
    11461 cctgcgtgtt actaaaggcg ctcgtgctgc tatcgaagct gctggcggta aaatcgagga
    11521 ataagtagca gatggctaaa caaccgggat tagattttca aagtgccaaa ggtggcttag
    11581 gcgagctgaa acgcagactg ctgtttgtta tcggtgcgct gattgtgttc cgtattggct
    11641 cttttattcc gatccctggt attgatgccg ctgtacttgc caaactgctt gagcaacagc
    11701 gaggcaccat cattgagatg tttaacatgt tctctggtgg tgctctcagc cgtgcttcta
    11761 tctttgctct ggggatcatg ccgtatattt cggcgtcgat cattatccag ctgctgacgg
    11821 tggttcaccc aacgttggca gaaattaaga aagaagggga gtctggtcgt cgtaagatca
    11881 gccagtacac ccgttacggt actctggtgc tggcaatatt ccagtcgatc ggtattgcta
    11941 ccggtctgcc gaatatgcct ggtatgcaag gcctggtgat taacccgggc tttgcattct
    12001 acttcaccgc tgttgtaagt ctggtcacag gaacgatgtt cctgatgtgg ttgggcgaac
    12061 agatcactga acgaggtatc ggcaacggta tttcaatcat tatcttcgcc ggtattgtcg
    12121 cgggactccc gccagccatt gcccatacta tcgagcaagc gcgtcaaggc gacctgcact
    12181 tcctcgtgtt gctgttggtt gcagtattag tatttgcagt gacgttcttt gttgtgtttg
    12241 ttgagcgtgg tcaacgccgc attgtggtaa actacgcgaa acgtcagcaa ggtcgtcgtg
    12301 tctatgctgc acagagcaca catttaccgc tgaaagtgaa tatggcgggg gtaatcccgg
    12361 caatcttcgc ttccagtatt attctgttcc cggcgaccat cgcgtcatgg ttcgggggcg
    12421 gtactggttg gaactggctg acaacaattt cgctgtattt gcagcctggg caaccgcttt
    12481 atgtgttact ctatgcgtct gcaatcatct tcttctgttt cttctacacg gcgttggttt
    12541 tcaacccgcg tgaaacagca gataacctga agaagtccgg tgcatttgta ccaggaattc
    12601 gtccgggaga gcaaacggcg aagtatatcg ataaagtaat gacccgcctg accttggttg
    12661 gtgcgctgta tattaccttt atctgcctga tcccggagtt catgcgtgat gcaatgaaag
    12721 taccgttcta cttcggtggg acctcactgc ttatcgttgt tgtcgtgatt atggacttta
    12781 tggctcaagt gcaaactctg atgatgtcca gtcagtatga gtctgcattg aagaaggcga
    12841 acctgaaagg ctacggccga taattggtcg cccgagaagt tacggagagt aaaaatgaaa
    12901 gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa tcgttaagcg tgatggtgtc
    12961 atccgtgtga tttgcagtgc cgagccgaag cataaacagc gccaaggctg attttttcgc
    13021 atatttttct tgcaaagttg ggttgagctg gctagattag ccagccaatc ttttgtatgt
    13081 ctgtacgttt ccatttgagt atcctgaaaa cgggcttttc agcatggaac gtacatatta
    13141 aatagtagga gtgcatagtg gcccgtatag caggcattaa cattcctgat cataagcatg
    13201 ccgtaatcgc attaacttcg atttatggcg tcggcaagac ccgttctaaa gccatcctgg
    13261 ctgcagcggg tatcgctgaa gatgttaaga tcagtgagct gtctgaagga caaatcgaca
    13321 cgctgcgtga cgaagttgcc aaatttgtcg ttgaaggtga tctgcgccgt gaaatcagca
    13381 tgagcatcaa gcgcctgatg gatcttggtt gctatcgcgg tttgcgtcat cgtcgtggtc
    13441 tcccggttcg cggtcagcgt accaagacca acgcacgtac ccgtaagggt ccgcgcaaac
    13501 cgatcaagaa ataatcgggg tgattgaata atggcaaagg caccaattcg tgcacgtaaa
    13561 cgtgtaagaa aacaagtctc tgacggcgtg gctcatatcc atgcttcttt caacaacacc
    13621 atcgtgacta tcactgatcg tcagggtaac gcgttgggtt gggcaacagc cggtggttcc
    13681 ggtttccgtg gttctcgcaa atccactccg tttgcagctc aggttgcagc agagcgttgc
    13741 gctgacgccg tgaaagaata cggcatcaag aatctggaag ttatggttaa aggtccgggt
    13801 ccaggccgcg aatctactat tcgtgctctg aacgccgcag gtttccgcat cactaacatt
    13861 actgatgtga ctccgatccc tcataacggt tgtcgtccgc cgaaaaaacg tcgcgtataa
    13921 cgcctcgttt tccaggtttg ttggagaaag aaaatggcaa gatatttggg tcctaagctc
    13981 aagctgagcc gtcgtgaggg caccgactta ttccttaagt ctggcgttcg cgcgatcgat
    14041 accaagtgta aaattgaaca agctcctggc cagcacggtg cgcgtaaacc gcgtctgtct
    14101 gactatggtg tgcagttgcg tgaaaagcaa aaagttcgcc gtatctatgg tgtgctggag
    14161 cgtcagttcc gtaactacta caaagaagca gcacgtctga aaggcaacac cggtgaaaac
    14221 ctgttggctc tgctggaagg tcgtctggac aacgttgtat accgtatggg cttcggtgcc
    14281 actcgtgcag aagcacgtca gctggttagc cataaagcaa ttatggtaaa cggtcgtgtt
    14341 gttaacatcg cttcttatca ggttagtccg aatgacgttg taagcattcg tgagaaagcg
    14401 aagaagcagt ctcgcgtgaa agccgctctg gagctggctg agcagcgtga aaagccaact
    14461 tggctggaag ttgatgctgg caagatggaa ggtacgttta agcgtaagcc ggagcgttct
    14521 gatctgtctg cggacattaa cgaacacctg atcgtcgagc tttactccaa gtaaagctta
    14581 gtaccaaaga gaggacacaa tgcagggttc tgtgacagag tttctaaaac cgcgcctggt
    14641 tgatatcgag caagtgagtt cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg
    14701 ctttggccat actctgggta acgcactgcg ccgtattctg ctctcatcga tgccgggttg
    14761 cgcggtgacc gaggttgaga ttgatggtgt actacatgag tacagcacca aagaaggcgt
    14821 tcaggaagat atcctggaaa tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg
    14881 caaagatgaa gttattctta ccttgaataa atctggcatt ggccctgtga ctgcagccga
    14941 tatcacccac gacggtgatg tcgaaatcgt caagccgcag cacgtgatct gccacctgac
    15001 cgatgagaac gcgtctatta gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc
    15061 ggcttctacc cgaattcatt cggaagaaga tgagcgccca atcggccgtc tgctggtcga
    15121 cgcatgctac agccctgtgg agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca
    15181 gcgtaccgac ctggacaagc tggtcatcga aatggaaacc aacggcacaa tcgatcctga
    15241 agaggcgatt cgtcgtgcgg caaccattct ggctgaacaa ctggaagctt tcgttgactt
    15301 acgtgatgta cgtcagcctg aagtgaaaga agagaaacca gagttcgatc cgatcctgct
    15361 gcgccctgtt gacgatctgg aattgactgt ccgctctgct aactgcctta aagcagaagc
    15421 tatccactat atcggtgatc tggtacagcg taccgaggtt gagctcctta aaacgcctaa
    15481 ccttggtaaa aaatctctta ctgagattaa agacgtgctg gcttcccgtg gactgtctct
    15541 gggcatgcgc ttggaaaact ggccaccggc aagcatcgct gacgagtaac cggatcacag
    15601 gttaaggttt tactgagaag gataaggtca tgcgccatcg taagagtggt cgtcaactga
    15661 accgcaacag cagccatcgc caggctatgt tccgcaatat ggcaggttca ctggttcgtc
    15721 atgaaatcat caagacgact ctgcctaaag cgaaagagct gcgccgcgta gttgagccgc
    15781 tgattactct tgccaagact gatagcgttg ctaatcgtcg tctggcattc gcccgtactc
    15841 gtgataacga gatcgtggca aaactgttta acgaactggg cccgcgtttc gcgagccgtg
    15901 ccggtggtta cactcgtatt ctgaagtgtg gcttccgtgc aggcgacaac gcgccgatgg
    15961 cttacatcga gctggttgat cgttcagaga aagcagaagc tgctgcagag taatctgaag
    16021 caatgtaaaa aaacccgccc cggcgggttt ttttataccc gtagtatccc cacttatcta
    16081 caatagctgt actctttt
//
LOCUS       DBKELA010000091        15410 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.091, whole genome shotgun sequence.
ACCESSION   DBKELA010000091 DBKELA010000000
VERSION     DBKELA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.091"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1175)
                     /locus_tag="ACUQR0_003034"
     CDS             complement(<1..1175)
                     /locus_tag="ACUQR0_003034"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9109980.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="ACUQR0_003035"
     tRNA            complement(1374..1458)
                     /locus_tag="ACUQR0_003035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="ACUQR0_003036"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="ACUQR0_003036"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008106 - alcohol dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HHV9109981.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="ACUQR0_003037"
     CDS             2802..4304
                     /locus_tag="ACUQR0_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HHV9109982.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="ACUQR0_003038"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="ACUQR0_003038"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HHV9109983.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="ACUQR0_003039"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="ACUQR0_003039"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HHV9109984.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6668..6718)
                     /gene="ytgA"
                     /locus_tag="ACUQR0_003040"
     CDS             complement(6668..6718)
                     /gene="ytgA"
                     /locus_tag="ACUQR0_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HHV9109985.1"
                     /translation="MSIIILATISTTQENK"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="ACUQR0_003041"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="ACUQR0_003041"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HHV9109986.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="ACUQR0_003042"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="ACUQR0_003042"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HHV9109987.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="ACUQR0_003043"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="ACUQR0_003043"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HHV9109988.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="ACUQR0_003044"
     CDS             complement(11869..13065)
                     /locus_tag="ACUQR0_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HHV9109989.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="ACUQR0_003045"
     CDS             13258..13761
                     /locus_tag="ACUQR0_003045"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9109990.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="ACUQR0_003046"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="ACUQR0_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HHV9109991.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="ACUQR0_003047"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="ACUQR0_003047"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HHV9109992.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3932 c         3966 g         3536 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcgtc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatca ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc tgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaaa gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca tacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 agcgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DBKELA010000092        15333 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.092, whole genome shotgun sequence.
ACCESSION   DBKELA010000092 DBKELA010000000
VERSION     DBKELA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.092"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(162..1187)
                     /gene="tdh"
                     /locus_tag="ACUQR0_003048"
     CDS             complement(162..1187)
                     /gene="tdh"
                     /locus_tag="ACUQR0_003048"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HHV9109993.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGVRNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="ACUQR0_003049"
     CDS             complement(1197..2393)
                     /gene="kbl"
                     /locus_tag="ACUQR0_003049"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HHV9109994.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="ACUQR0_003050"
     CDS             complement(2668..3525)
                     /gene="yibB"
                     /locus_tag="ACUQR0_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HHV9109995.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3829..4761
                     /gene="rfaD"
                     /locus_tag="ACUQR0_003051"
     CDS             3829..4761
                     /gene="rfaD"
                     /locus_tag="ACUQR0_003051"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HHV9109996.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4771..5817
                     /gene="rfaF"
                     /locus_tag="ACUQR0_003052"
     CDS             4771..5817
                     /gene="rfaF"
                     /locus_tag="ACUQR0_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HHV9109997.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5821..6813
                     /gene="rfaC"
                     /locus_tag="ACUQR0_003053"
     CDS             5821..6813
                     /gene="rfaC"
                     /locus_tag="ACUQR0_003053"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HHV9109998.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6810..8018
                     /gene="rfaL"
                     /locus_tag="ACUQR0_003054"
     CDS             6810..8018
                     /gene="rfaL"
                     /locus_tag="ACUQR0_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HHV9109999.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8054..9196)
                     /locus_tag="ACUQR0_003055"
     CDS             complement(8054..9196)
                     /locus_tag="ACUQR0_003055"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HHV9110000.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9205..10218)
                     /locus_tag="ACUQR0_003056"
     CDS             complement(9205..10218)
                     /locus_tag="ACUQR0_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HHV9110001.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="ACUQR0_003057"
     CDS             complement(10243..10950)
                     /gene="rfaY"
                     /locus_tag="ACUQR0_003057"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HHV9110002.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="ACUQR0_003058"
                     /gene_synonym="rfaI"
     CDS             complement(10976..11983)
                     /gene="waaO"
                     /locus_tag="ACUQR0_003058"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HHV9110003.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="ACUQR0_003059"
     CDS             complement(12026..12823)
                     /gene="rfaP"
                     /locus_tag="ACUQR0_003059"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HHV9110004.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12816..13940)
                     /locus_tag="ACUQR0_003060"
     CDS             complement(12816..13940)
                     /locus_tag="ACUQR0_003060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHV9110005.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13937..14995)
                     /gene="rfaQ"
                     /locus_tag="ACUQR0_003061"
     CDS             complement(13937..14995)
                     /gene="rfaQ"
                     /locus_tag="ACUQR0_003061"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HHV9110006.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4376 a         3445 c         3142 g         4370 t
ORIGIN      
        1 tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca gctatataaa
       61 agatcagcgc aattacgaac tctttattac actgacagga ttttacataa ttaacagatt
      121 atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca gctcagaata
      181 actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc atcgatagag
      241 aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc cgccatcttg
      301 taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc tttaaagatc
      361 actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc aatacgaccg
      421 ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga catttccaga
      481 ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag gttttctttc
      541 gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg gtattcgtta
      601 acatcggtga tcaccacatt gcgtacacca acgtgtttcg ccaccgccgc agccataata
      661 cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa cgacagcgcg
      721 gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga gatattgtcg
      781 gggattttgg aagcgttgaa cgccggaatc accagatatt cggcgaagca gcccggacgg
      841 ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg acagttgcga
      901 cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt gaagcctttc
      961 acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac catcggcacc
     1021 gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt cccgcagatg
     1081 gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac agggacgtcg
     1141 gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc acatcctcag
     1201 gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg cgtaatttgc
     1261 tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt cggaacgacc
     1321 ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc aaatttctgc
     1381 gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc cgccagggta
     1441 aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag acggtcacgc
     1501 aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac aattgctggt
     1561 gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc aaccacttct
     1621 ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag cgtgccggtg
     1681 atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc attttcaccg
     1741 acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt atctgccaga
     1801 tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc atcggtggca
     1861 atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc aagctcctgc
     1921 atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc atcaataata
     1981 gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag cgtttcaaac
     2041 aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat ccccaggaat
     2101 gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat aaaacgcacc
     2161 gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat cagatccgga
     2221 tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct tccatcagcc
     2281 acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa caacccttcc
     2341 gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg cattgcgatt
     2401 ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg acgagatgat
     2461 gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc tctcaacacc
     2521 tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg attttactgc
     2581 attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca gttggcatca
     2641 aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc tcagcgcctg
     2701 catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc attttcctct
     2761 ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga caaaaatgcc
     2821 ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca gaactaattt
     2881 atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa tatatacatg
     2941 attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc ctttttttat
     3001 agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca atcctcgagt
     3061 tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg ttttcactaa
     3121 acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca aaacatactc
     3181 tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg taaatgactc
     3241 atctttttga attttttcga tacgacttct gatatatcta aatttctttt ttatatcgat
     3301 aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt tcaaatcggg
     3361 agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt aactaaaata
     3421 aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc aatcccctct
     3481 accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta ataagaccat
     3541 taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta tattatccat
     3601 tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac tattcacatg
     3661 caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt tgcaattagc
     3721 atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat gattacagac
     3781 attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat gatcatcgtt
     3841 accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga taaaggcatc
     3901 accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa cctggtggat
     3961 ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat ggctggcgaa
     4021 gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac caccgagtgg
     4081 gatggcaagt atatgatgga taacaactat caatactcca aagagctgct gcactactgt
     4141 ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg cggacgcacc
     4201 tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg ttactcaaaa
     4261 ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca gattgttggc
     4321 ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat ggcgagcgtc
     4381 gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt cgaaggtagc
     4441 gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa cctgtggttc
     4501 ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga atccttccag
     4561 gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata cattccgttc
     4621 ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac aaatctgcgc
     4681 gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga atacatggcc
     4741 tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg cccgtcttgg
     4801 gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg ctatccccag
     4861 gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg gatgccggaa
     4921 gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg cgaacgccgc
     4981 aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt accaaactcc
     5041 ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg ctggcgcggc
     5101 gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc ctggccgcta
     5161 atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac cgcacaagat
     5221 ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa atcatatacc
     5281 tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc gggtgcggag
     5341 tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa gcagctgatt
     5401 gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc gggcaatgag
     5461 attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc gggggaaaca
     5521 cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac taacgattct
     5581 ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta tggtccaagt
     5641 agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct gattaccggc
     5701 tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt gatcgacatt
     5761 actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga agcctgacgg
     5821 atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac gttgcccgca
     5881 ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt ggaagaaggg
     5941 ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt ggcaatacgt
     6001 cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc gtttcgtgaa
     6061 gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt aaaaagcgcg
     6121 gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca aaccgctcgc
     6181 gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca gcagcacgcc
     6241 gtagaacgca cccgcgaact gtttgctaaa agtttgggct atagcaaacc gcaaacccag
     6301 ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc tggcgaatat
     6361 gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga acactggcga
     6421 gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg gggcgcgccg
     6481 catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga agtattgccg
     6541 aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt agtgtcggtg
     6601 gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac ggtttatgga
     6661 ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg tcgttctaca
     6721 agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa tcttgaaata
     6781 ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc tctctcgaga
     6841 aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt accacctatt
     6901 ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca ataacgacaa
     6961 tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca tttctttttg
     7021 gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc cctgatgcag
     7081 gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta tgcacaatag
     7141 caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc gttttcttct
     7201 catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat tataaggact
     7261 atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct gactcgatgg
     7321 tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa taccgcattt
     7381 cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta tccagagggg
     7441 cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa caatggaagt
     7501 tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc acacataagg
     7561 agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat cgctatgcaa
     7621 atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt ggttatggtt
     7681 acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat ccagaatgga
     7741 cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt ggcactggtt
     7801 tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag tgtataaaaa
     7861 atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc ttactatctt
     7921 ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg
     7981 tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat
     8041 gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc
     8101 catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact gctgttaatt
     8161 caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt
     8221 ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca
     8281 ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga
     8341 actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc ttttcaggcg
     8401 gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc
     8461 gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt
     8521 ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt
     8581 tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag atattaagtt
     8641 cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta
     8701 caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag tataaactag
     8761 ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta
     8821 taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc atgctattat
     8881 gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct
     8941 gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta tatatgcgac
     9001 tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt gagtatcctg
     9061 gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc tgatacatcc
     9121 acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta accgtaaata
     9181 ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa ttgctgtaaa
     9241 aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt tgtgctcatg
     9301 ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat aaatattacg
     9361 aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta taccagtata
     9421 atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat ttttttcttc
     9481 aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt tagcatgatg
     9541 tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta taattgactc
     9601 atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt cccgtaaatt
     9661 aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac acaaacgctc
     9721 agcgctttta ctttgcatag catctacatc aattaccact gccccatatt catctttaaa
     9781 ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat caagatataa
     9841 taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat aaatagatac
     9901 tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat gatataattt
     9961 aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact ctttgtctaa
    10021 gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa gaattgaagc
    10081 aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga cattaaacgt
    10141 ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt taaattgagt
    10201 aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc tcttcaatcc
    10261 tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata taagatatgt
    10321 atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat caatgcgatc
    10381 ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc ttaaaccttt
    10441 ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat gctgatgcaa
    10501 ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca aatactcatt
    10561 taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga aatttaatgt
    10621 tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac cacgtacccg
    10681 atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt ttaagaaacg
    10741 ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac gagcagtgtc
    10801 aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt taaccctgca
    10861 agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta tgaaaacttt
    10921 caacccattt atcgttttat tatatatcat aatgaattat tttaacctta aatctttaga
    10981 agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa tgcttttgtt
    11041 taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta agtaaatctt
    11101 ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc ggataatcag
    11161 cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg tcatcattga
    11221 ctggattatc aactttgtct tttaattcat agttaatact atatcgggta ttatatttct
    11281 ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga tcaaggtggg
    11341 tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac gaaatgttat
    11401 ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat ccagaagcta
    11461 actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct ctttcagcta
    11521 caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta atactaccct
    11581 tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag aaataatctg
    11641 caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt aatgacttca
    11701 gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt gctaaatcag
    11761 aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat tcacaagaga
    11821 tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa agaaaatttc
    11881 tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga taatgaaaat
    11941 tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt ctgtatccaa
    12001 aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt tccctgattt
    12061 ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga atgtctttaa
    12121 gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc aggccaatat
    12181 tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct ggaacgcgcg
    12241 tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct tccttaccgg
    12301 aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta atgcccgcag
    12361 catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt acgcgaacat
    12421 ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt ggtgtcagat
    12481 cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg ccgaatgcca
    12541 ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc cattcgcggt
    12601 ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct ttcagggtcg
    12661 tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc agagtacggc
    12721 gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca aaaggatctt
    12781 tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc acccgttatg
    12841 atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata atgtcgcgca
    12901 ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa aatctcattc
    12961 aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc gacaatataa
    13021 tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat cgcttccagc
    13081 agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc cgccgccatt
    13141 aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac gccgcgtttt
    13201 tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag caatgtgttg
    13261 tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc acctttacgc
    13321 gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc attcttctta
    13381 cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg ataaatcccc
    13441 ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa atcggcgatt
    13501 tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt tgcccgctca
    13561 aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt ttcctgcgct
    13621 actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg cattttgttg
    13681 aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca ggcaaaatac
    13741 tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac ttcaaataca
    13801 tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc tcgggcggcg
    13861 actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa atatttatat
    13921 aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt aatttatcga
    13981 cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt cggtcacgct
    14041 gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg ttatttgacc
    14101 agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc gtattaactg
    14161 cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca tgatcgatta
    14221 acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt ggcgtctggc
    14281 atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg gacgtcagaa
    14341 cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg gcgttgtccc
    14401 agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt ccaacaccag
    14461 cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc atggttgtct
    14521 gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt tccaccacat
    14581 ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca gactgccgat
    14641 gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt atagcaacca
    14701 tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat accttgatga
    14761 gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt atgccgtaga
    14821 gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa agcagcacat
    14881 cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta gttaataaca
    14941 tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta tccacaagta
    15001 actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct accgccccag
    15061 gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt gtatcacttc
    15121 tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca ataaagaaat
    15181 caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga ttccttcctg
    15241 cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag atgttcagca
    15301 agaattggcg ggtaaattgc tatttaagtc aaa
//
LOCUS       DBKELA010000093        15254 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.093, whole genome shotgun sequence.
ACCESSION   DBKELA010000093 DBKELA010000000
VERSION     DBKELA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.093"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..73)
                     /locus_tag="ACUQR0_003062"
     CDS             complement(<1..73)
                     /locus_tag="ACUQR0_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HHV9110007.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACUQR0_003063"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="ACUQR0_003063"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HHV9110008.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="ACUQR0_003064"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="ACUQR0_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HHV9110009.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACUQR0_003065"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="ACUQR0_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HHV9110010.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="ACUQR0_003066"
     CDS             2703..2939
                     /locus_tag="ACUQR0_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HHV9110011.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="ACUQR0_003067"
     CDS             2942..3799
                     /locus_tag="ACUQR0_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HHV9110012.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5796
                     /locus_tag="ACUQR0_003068"
                     /pseudo
     CDS             3799..5796
                     /locus_tag="ACUQR0_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="ACUQR0_003069"
     CDS             complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="ACUQR0_003069"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HHV9110013.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6399..7295)
                     /locus_tag="ACUQR0_003070"
     CDS             complement(6399..7295)
                     /locus_tag="ACUQR0_003070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHV9110014.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7344..7583)
                     /locus_tag="ACUQR0_003071"
     CDS             complement(7344..7583)
                     /locus_tag="ACUQR0_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HHV9110015.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7686..8285
                     /gene="nemR"
                     /locus_tag="ACUQR0_003072"
     CDS             7686..8285
                     /gene="nemR"
                     /locus_tag="ACUQR0_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HHV9110016.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8322..9419
                     /gene="nemA"
                     /locus_tag="ACUQR0_003073"
     CDS             8322..9419
                     /gene="nemA"
                     /locus_tag="ACUQR0_003073"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HHV9110017.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQLGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9500..9907
                     /gene="gloA"
                     /locus_tag="ACUQR0_003074"
     CDS             9500..9907
                     /gene="gloA"
                     /locus_tag="ACUQR0_003074"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HHV9110018.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10010..10657
                     /gene="rnt"
                     /locus_tag="ACUQR0_003075"
     CDS             10010..10657
                     /gene="rnt"
                     /locus_tag="ACUQR0_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HHV9110019.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10750..15208
                     /locus_tag="ACUQR0_003076"
                     /pseudo
     CDS             10750..15208
                     /locus_tag="ACUQR0_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT         3502 a         4029 c         4015 g         3708 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccggctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gcaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tacttttggt caaatttggc ctgatggtcc agattagcga tctgtggcaa
     5101 tttttactgt ttctctttcc actgctggcg acaatgcagc ttcttaaatt gcagatgccc
     5161 aaatttgccg cactgtgggg gcaactgatt gtttttatgg gttcgtttat cgctgtcact
     5221 aatcccccgg tgtatgattt tgctgatttt cttaactata atctggcaaa aatcgttggc
     5281 gttgcgctgg cttggctagc gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc
     5341 cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg ccatccaaca
     5401 ctgagtgaaa gcgaatttga atcgctcact tatcatcacg tcagtcagtt gagtaacagc
     5461 caggatgcgc tggctcgccg ttggttatta cgctggggtg tagtgctgct gaactgttct
     5521 catgttgtct ggcaattgcg cgactgggaa tcgcgttccg atccgttatc gcgagtacgg
     5581 gataactgta tttcactgtt gcggggagtg atgagtgagc gtggcgttca gcaaaaatca
     5641 ctggcggcca cacttgaaga attacagcgg atttgcgaca gccttgcccg tcatcatcaa
     5701 cctgccgccc gtgagctggc ggcaattgtc tggcggctgt actgctcgct ttcgcaactt
     5761 gagcaagcac caccgcaagg tacgctggcc tcgtaattac ttaattacac cacaggcata
     5821 gcgttcaccg ccaccgccca gcggtttagg ttgatcggac atattatcgc cgccaacgtg
     5881 gaccatcagc gccttgtctt tgatttcatc cagtgatttc agacgaggcg cgatgacggc
     5941 atcggtagct ttgccgtcat tattgacgac cagtgcaggc agatcgccta aatgcccggt
     6001 accttctggc ccttcatgtt taccggtatt ttgtggatca agatgcccgc ctgcggattc
     6061 cgcggcgctg gctttgccat ctttggtggc tggctggcag cttcctttgg catgaatatg
     6121 gaagccatgt tcaccggggg gtaatgcttt cagatcgggt gaaaactcca gacctttatc
     6181 ggtttcagta atggtgacgc taccaattga ctgcccaacc ccttgcgacg tgacgaggtt
     6241 catctcgact ttttcactgg cagcttgtgc gccggtcgca acaaccagcg ccagaatggc
     6301 cagactaaaa cgtttcatag gacctccgtt cattgtgcat tcctgaataa gtgtaaacca
     6361 gtgacacatt tttgaacggc ggctattcct aaaagtgctt acggtacgtc gtaccccagt
     6421 gcagctttac ggatacgaaa ccattgttga cgggtcattt tcagtgtttc tgcttcgaca
     6481 gctgcccgta cgcgctcaat tttacctgag ccgataattg gcagcggctg cgacggtaaa
     6541 cgtaataccc aggcgtaaac cacctgttca atcgagcccg cgtttaactc ctccgccacc
     6601 acagccagtt catcacgcag cggctggaaa taatcatcat taaacagacg accaccacca
     6661 aggcaggacc acgccatcgg acgaacacgc agttgttgta gttggtcgag cgtgccatcc
     6721 agcagtaacg gctgatgcac cggggatatt tccacctgat tagtggcaag ggtaaacggc
     6781 agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc
     6841 actttgccgc tctgatgcag atgtttgaac gcgtccgcca cttcatcggc atccattaac
     6901 gggtctggtc ggtggattaa cagcagatcc agatgatcgg tcgcgagatt aattagcgac
     6961 tgttcggcgc tcttaatgat gtgattgcgg tcagtgatgt aatgaccaat gacgttttct
     7021 tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt ccatccgttc acgcaggtga
     7081 ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact gatagccacc ataaatatca
     7141 gcatggtcca cggtggtcac gccgagatcc agatgctctt caataaaact gaccagctgg
     7201 cgggcggaca tattccagtc catcaatcgc cagtagccca tcacaaaacg ggaaaactcc
     7261 gggccttccg gcgcaatagt aatacgctga accataatcg cttcctctta tcagatataa
     7321 gaggagtata cgcaagatta ggttcaaaag agtgatggtt gctccggttc gtctgatgac
     7381 gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct
     7441 tgtttttcgc catcgctcat tttattccag ttaaaacgct catcccgact acgaaaacag
     7501 ccgcgacaaa acccgcgttc gtcagactgg caaattcccc ggcacgggct ctggacggga
     7561 aagaactcta attgctccgc cacctcgccc tcctcagata agattattac cattattgaa
     7621 gctgttaatg tccaaagtag caactttgct tgcactagac cgactggtct actacactcc
     7681 aacgcatgaa caaacacacc gaacatgata ctcgcgaaca tctcttggcg acgggcgagc
     7741 aactttgcct gcaacgtgga ttcaccggga tggggctaag cgaattacta aaaaccgctg
     7801 aagtgccgaa agggtccttc tatcactact ttcgctctaa agaagcgttt ggcgttgcca
     7861 tgcttgagcg tcattacgcc gcatatcacc agcgactggc tgagttgctg caatccggcg
     7921 aaggtaacta ccgcgaccgc atactggctt attaccagca aacactgaac cagttttgcc
     7981 aacatggaac catcagtggt tgcctgacag taaaactctc tgccgaagtg tgcgatctgt
     8041 cagaagatat gcgcagcgcg atggataaag gtgctcgcgg cgtgatcgcc ctgctctcgc
     8101 aggctctgga aaatggccgt gagaaccatt gtttaacctt ttgtggcgaa ccgctacaac
     8161 aggcacaagt gctttacgca ctatggcttg gcgcgaatct gcaggccaaa atttcgcgca
     8221 gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa cattattgcg acgcctgccg
     8281 tttagcaggc attttttatc accagacgac cgggagcctt tatgtcatct gaaaaactgt
     8341 attccccact gaaagtgggc gcgatcacgg cggcaaaccg tatttttatg gcaccgctga
     8401 cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc gttgatggcg gaatactatc
     8461 gccaacgtgc cagtgccggt ttgattatta gtgaagccac acaaatttct gcccaggcaa
     8521 aaggatatgc aggtgcgcct ggcatccata gtccggagca aattgccgca tggaaaaaaa
     8581 tcaccgctgg cgttcatgct gaaaatggtc atatggcctt gcagctgtgg cacaccggac
     8641 gcatttctca cgccagcctg caacttggcg gtcaggcacc ggtagcgcct tcagcactta
     8701 gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca ggcgatccgc gttgaaacat
     8761 ccatgccgcg tgcgcttgaa ctggaagaga ttccgggtat cgtcaatgat ttccgtcagg
     8821 ccattgctaa cgcgcgtgaa gccgcttttg atctggtaga gctccactct gctcacggtt
     8881 atttgctgca tcagttcctt tccccttctt caaaccatcg taccgatcag tacggcggca
     8941 gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga tgccgggatt gaagaatggg
     9001 gtgccgatcg cattggcatt cgcgtttcgc caatcggtac tttccagaac acagataacg
     9061 gcccgaatga agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg
     9121 cttatctgca tatgtcagaa ccagattggg cggggggtga accgtatact gatgcgttcc
     9181 gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacagtag
     9241 aaaaagctga aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact
     9301 ggattgcgaa cccggatctg gtcgcccgct tgcagctcaa agctgagctt aacccacagc
     9361 gtgccgaaag tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat
     9421 ccaacattgc gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag
     9481 ccattttgag gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca
     9541 acgctccatc gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa
     9601 cccggaatac aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt
     9661 gattgaactg acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca
     9721 catcgcgctt agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg
     9781 taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga
     9841 agatccggac ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg
     9901 caactaatct cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt
     9961 aatatttgtc ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa
    10021 cgctcaactt accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt
    10081 tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct
    10141 gaaaatggat gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc
    10201 atttgtcggc gcaaatttgc aaccagaagc cctcgccttc aacggcattg acccgaacga
    10261 tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg
    10321 taaaggtatt aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca acgccaattt
    10381 tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca
    10441 ccctttcgcc acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt
    10501 gtcaaaggct tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct
    10561 gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct
    10621 gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg
    10681 caatgattca ggcatctata gtgaggctat tccacgcatc aagcatgata ttcacgggga
    10741 atagcgttaa tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcaccg
    10801 gcgacccgcg actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc
    10861 tggcatgtgg cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa
    10921 acgctggcag catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc
    10981 cgcgaggcgc ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg
    11041 ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga tgaacggcgg
    11101 cggcgcggcg aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt
    11161 gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga
    11221 attggccttt ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcgat
    11281 cgcccggtta cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta
    11341 ccggtcgcca atatggatga tgtctcatcg gtcgccagcg gcaccggcga agacagccat
    11401 gccggccggg aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg
    11461 cgccatcgct cgaccattgt ctttactaat tcacgcgggc tggcggaaaa actgacagca
    11521 cgattaaatg agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg
    11581 gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt
    11641 attgcccgct cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgaacaggcg
    11701 ctgaaatcgg gtgaattacg ctgcgtggtc gcaacctcca gtcttgaact ggggattgat
    11761 atgggcgcgg tggatctggt gattcaggtg gcaacgccgc tttctgttgc cagtgggtta
    11821 caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttcccc
    11881 cgtacccggc gtgatttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg
    11941 ctggaaaacc tgacaccacc gcataatcct ctcgacgtcc ttgcgcagca aaccgttgcc
    12001 gccgcggcga tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtaccgca
    12061 ccgtggaaag atctgccaag acgtgttttt gacgccacgc tggatatgct ttccgggcgc
    12121 tatccctctg gcgatttttc tgcttttcgc cccaaactgg tctggaacag ggagaccggg
    12181 atattgaccg cccgacctgg cgctcaattg ttggcggtta ccagcggcgg caccattccg
    12241 gatcgtggca tgtatagcgt gttattaccc gaaggtgaag aaaaggccgg ttcgcggcgg
    12301 gtgggtgaac tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc
    12361 ggcgctacct catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct
    12421 ccgggtcgtt ctgcccagct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa
    12481 ttaggcgaga tgatcggcga ttttcttcat ttactggcgg atggcgcgtt cttttccggg
    12541 actattcccc cgtggctggc agaagaaaat acgaacgcca atattcaggg gttgattgac
    12601 gagcaacgca acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc
    12661 cgtgatgaaa ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat
    12721 gaaccctggg cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct
    12781 gtggtcgcca gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc
    12841 gacgccgcga tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta
    12901 ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg
    12961 ccgggccgca cgccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag
    13021 ttgctggaaa tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa
    13081 tgtctgcaag atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt
    13141 ggcgaaattc aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta
    13201 ttgttcggct atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg
    13261 gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg
    13321 ggggaattac tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct
    13381 cctggtagaa gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca
    13441 atgaccgttg aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat
    13501 ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt
    13561 cttgcctgta tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg gctaccagag
    13621 tcattgccag agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc
    13681 tatctccggg ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt
    13741 attgccattg tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg
    13801 caacaaaaca tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa
    13861 gccgccagag aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa
    13921 cgtcagggcg tattacccgc caccgatggt agcccggcgc tctttgcctc aacatctcca
    13981 ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt
    14041 ttacccgcct cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgcca
    14101 gaaatgctcg atgaattact ggcaaccgga gcggttatct ggtcgggaca aaaaaaagct
    14161 gggtgaagat gacggcctgg tggcactgca tctacagaaa tatgccgcag aatcgttcac
    14221 tcccgccgaa gcggatcagg cgaatcgttc ggcgctgcag caagcgataa tcgctgttct
    14281 ggctgacgga ggagcctggt ttgcacaaca aatcagccaa cggatacgcg ataaaatcgt
    14341 cgaatcggtt gattcctcgg cactgcaaga ggcgttatgg gcgctggtct ggcaaggcgt
    14401 cattaccagc gatatttggg caccgttacg cgccctcacc cgcagcagtt ccaacgcacg
    14461 cacctcaact cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg cacaacccgt
    14521 ctcgccgcgg gtatcttaca acacaccaaa tctggctgga cgctggtcgt tattgcaggt
    14581 ggagccacta aacgataccg aaaggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    14641 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    14701 gcaaacactt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    14761 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    14821 gacacaagcc acgcaaacac gccactatac accagtggcg ctctccgcca acgatccggc
    14881 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg taccaacgcg
    14941 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    15001 cggcaaaaaa atgctggtct ggcaggaaaa ggaggaatta ctcgccccag agattttcca
    15061 ggcgctgacc accgcactgc gtcgcgaacc aaggctgcgc tttacgctaa cagaagtgaa
    15121 tgatcttccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    15181 ttcgccacaa gggctggatt ggggatagtt gacatcctcc acgccctgaa agacgtggtt
    15241 ccctgctacg ttca
//
LOCUS       DBKELA010000094        15096 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.094, whole genome shotgun sequence.
ACCESSION   DBKELA010000094 DBKELA010000000
VERSION     DBKELA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.094"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..177
                     /gene="yjbD"
                     /locus_tag="ACUQR0_003077"
     CDS             <1..177
                     /gene="yjbD"
                     /locus_tag="ACUQR0_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HHV9110020.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(310..1182)
                     /gene="rluF"
                     /locus_tag="ACUQR0_003078"
     CDS             complement(310..1182)
                     /gene="rluF"
                     /locus_tag="ACUQR0_003078"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HHV9110021.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1530..2477
                     /locus_tag="ACUQR0_003079"
     CDS             1530..2477
                     /locus_tag="ACUQR0_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HHV9110022.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2547..3353
                     /locus_tag="ACUQR0_003080"
     CDS             2547..3353
                     /locus_tag="ACUQR0_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9110023.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3363..3770
                     /locus_tag="ACUQR0_003081"
                     /pseudo
     CDS             3363..3770
                     /locus_tag="ACUQR0_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
     gene            3770..4264
                     /locus_tag="ACUQR0_003082"
     CDS             3770..4264
                     /locus_tag="ACUQR0_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HHV9110024.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4330..5127
                     /locus_tag="ACUQR0_003083"
     CDS             4330..5127
                     /locus_tag="ACUQR0_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HHV9110025.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5138..5926
                     /locus_tag="ACUQR0_003084"
     CDS             5138..5926
                     /locus_tag="ACUQR0_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HHV9110026.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5978..7207
                     /gene="sorE"
                     /locus_tag="ACUQR0_003085"
     CDS             5978..7207
                     /gene="sorE"
                     /locus_tag="ACUQR0_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HHV9110027.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7589..8278
                     /gene="pepE"
                     /locus_tag="ACUQR0_003086"
     CDS             7589..8278
                     /gene="pepE"
                     /locus_tag="ACUQR0_003086"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HHV9110028.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8369..10000)
                     /locus_tag="ACUQR0_003087"
     CDS             complement(8369..10000)
                     /locus_tag="ACUQR0_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HHV9110029.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10220..13903)
                     /gene="metH"
                     /locus_tag="ACUQR0_003088"
     CDS             complement(10220..13903)
                     /gene="metH"
                     /locus_tag="ACUQR0_003088"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HHV9110030.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14103..14927
                     /gene="iclR"
                     /locus_tag="ACUQR0_003089"
     CDS             14103..14927
                     /gene="iclR"
                     /locus_tag="ACUQR0_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HHV9110031.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3590 a         3981 c         3936 g         3589 t
ORIGIN      
        1 gtactgacca actccgaaac caacagcatt aagaaagagt acatcgataa attaatggtc
       61 gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga aaaagcaggc ttataaaccg
      121 gatcctgaag cgtcatttag ctggtcagca aatacgtcga cgcgcggcag gcgctaatga
      181 acaatcaacg cctgagattt tcactcgtct gtgtggacac aatcttcccc tcaccctaac
      241 cctctccccg aaggggcgag gggactgtcc gagctctttt tggacgtttt catcagtctg
      301 aggcgttaat cagcgtccct tctttttacg ccccggcgag gtaaaacgct taccgttgga
      361 cgccgggcga ccgccttttt ccgccgtttt ttccatctta acgactggac gtttgatgcc
      421 cgctgttttc ggtttggcct tcgctttagg ttttgcctcg gaagaggaat tttcgatgag
      481 cttaaagagg tcgattaact catcgtcggt taaatagcgc cactccccca gcggaatgcc
      541 gcttaagctg acgttcataa tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg
      601 ctcgcacatg cgccggatct gacggttcag cccctgcact agggtaatgc ggaagacaaa
      661 cggcgcttct tttttgactt tgcacttttt cgtcaccgtc ccgaggatcg gcacccccgc
      721 ccccatacca cgaataaatt catcggtaat cggtttatcg accgtcacca gatactcttt
      781 ctcatgatcg ttacccgcac ggaggatctt attcaccaga tcgccgtgat tggtgaggaa
      841 aatcagcccc tgggagtctt tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt
      901 aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa taccgacggg
      961 cttgttcagg gcgataagta ccaaatcttc ggcttcccga ggctcaatca actgaccgtt
     1021 tactttcaca atgtcgccgg gtttcacctg atcgccaatg gtggctcgct tgccattaag
     1081 gaacacattg ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc
     1141 gctgatgtat ttatttaaac ggactgatga gtcgggcagc ataaattctc ctgtaaaacg
     1201 cggaatatac cgtacctctt agcggggaaa aacagggtta tgcggcaacg tcgaaattaa
     1261 atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg aagtacgatg caaatttgcc
     1321 tggcaaaata aggataatca acatattacg aatcgctgca cacctgtgct gatgactact
     1381 acgcagagaa acaaatgtgc aagagaaagc gctatttctc cccatgcaaa gagtgaaaaa
     1441 tcgctttgaa aggccctcat gatggggtta tatcagatca agcggtgaga aagcgtattt
     1501 gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag tgacgatatc cgtttgattg
     1561 tgaagattgc ccaactctat tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac
     1621 tgggtattta ccgcaccacc atcagccgct tgcttaaacg aggccgcgat cagggaatgg
     1681 tcaccatcgc catcaactat gactacaacg aaaatctctg gctggagcag caactgaagc
     1741 aaaagtttgg cctgaaagac gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc
     1801 aactggcgat gatggggtta cacggcgcgc aactgctgga tcgcttgctg gagcctggcg
     1861 atattgtcgg tttttcctgg ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcagg
     1921 cggggcaatc gcggcagtta atctgcgtac caattattgg cggaccgtcc ggtaaactcg
     1981 aaagccgcta tcatgtaaac acattaacct acagcgcggc agcgaagctg aaaggggaat
     2041 cgcatctcgc ggattttccg gctctgctgg ataacccatt aattcgtaat gggatcatgc
     2101 agtctcagca ctttaaaacc atctctgcct actgggataa tctggatatc gccctggtgg
     2161 gaattggctc accggccatt cgcgacggcg ctaactggca tgcgttttat ggtggtgaag
     2221 agagtgacga cctgaatgcc cgccaggttg ctggcgatat ttgctcgcgc ttttttgata
     2281 ttcacggcgc aatggttgaa acgaatatga gcgaaaaaac gctctctatc gaaatgaata
     2341 aattaaagca ggcgcgatat tccattggca ttgccatgag tgaagaaaaa tacagcggaa
     2401 ttattggtgc actgcgtgga aaatatatta attgtctggt aacgaatagc agcacagctg
     2461 aactattact gaaataaaaa tcttcccgat gtttaccgta ataaaaatta cggcgggtcc
     2521 ctattatcta aaatcaggag tctgttatgc aaacgtggtt aaatttgcag gataaaatca
     2581 ttattgtcac cggcggcgca tccggtattg gtctggcgat tgtagaggaa ttattagcac
     2641 aaggcgcgaa tgtacagatg gtcgatattc acggtggcga tggtcaatat gaaggtcata
     2701 aaggctatca gttttggccg accgatattt ctagcacaaa agaggtaaat catacggtag
     2761 cggaaattat ccagcgtttt ggtcgcatcg acggtctggt caataacgcc ggggtcaatt
     2821 tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca gtatgaactc aacgaagctg
     2881 cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt tctgatgtcg caggcggtgg
     2941 cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa tgtttcctca gaaagtgggc
     3001 tggaaggctc agaaggacaa agctgttacg ccgctaccaa agccgcgctc aatagcttca
     3061 cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg tgtggtcggt atcgcgcccg
     3121 ggattctgga aaaaaccggg ctacgcacgc cggaatatga agaggcgctg gcgtggacgc
     3181 gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa aaacgccatt cctattgggc
     3241 gcgccggaag attagcagaa gtagctgatt ttgtttgtta tctgttgtct gaacgcgcca
     3301 gctacatcac cggagtaacc actaacattg cgggcggcaa aacgcgcggc taaggaggct
     3361 ttatggtcaa cgccattttt tgtgcccacg gcaaattagc ttgcgccatg ctggaatcgg
     3421 ttcagatggt ttacggcaac gccaatgttg aagccgttgc atttgtgccc ggtgaaaacg
     3481 ccggagacat tgcggtaaag ctggagaagt tagtaagcat tcacaatcaa gatgagtggc
     3541 tgattgccgt ggatttacag tgcggcagcc cgtagaatgc ggcagcaatg cttgcgatgc
     3601 gcaatcctcg tttgcgggta atcagcggcc tttctctgcc gctggcgctc gaactggttg
     3661 ataaccagga cagcatgaat gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc
     3721 aaacctgcgt tgtctggaaa caactggcaa cgacagagga ggatttctga tgaacattac
     3781 gctcgcccgc attgatgacc gtctgatcca cggccaggtc actaccgtct ggtcgaaagt
     3841 ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt tataacgatg aagtgcgccg
     3901 gacattattg cgtcaggctg cgccaccggg aatgaaggtt aacgtggtca atattgaaaa
     3961 agccgtcgcg gtttatcaca acccgcaata tcaggacgaa accgtttttt atcttttcac
     4021 tcgcccacag gatgccttag cgatggttcg ccagggggta aaaataggca ccctgaatat
     4081 tggcggcatg gcctggcggc ccggtaaaaa acagttaacc aaagcggttt cattagatga
     4141 tgatgatatt aacgcatttc atgagttaaa taatcttggt gtcatactgg atttacgcgt
     4201 cgtagcgtca gatccctcaa tcaatattat cgacaaaata aacgaacagt taatcgcaaa
     4261 ttaaataata gcgcctgtgt acatatgctt taacaggcat ggatgacgac acttaaaagg
     4321 tgacaaatca tggaaataag taccctacaa atcattgcta tatttctttt ttcctgtatt
     4381 gccggaatgg gcagcgtgct ggatgaattt cagactcatc gcccgttaat tgcctgtacg
     4441 gtgattggtt taattctcgg tgatttaaaa accggaataa tactcggcgg tacgctggaa
     4501 ttgatagctc tctgctggat gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc
     4561 agcataatct ccgccattct ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc
     4621 atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg tgtttgcccg taccatcacc
     4681 gtagtgttcc agcacgcggc ggataaagca gcagaagagg cgcggtttcg cactctcgat
     4741 attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg tcgccattcc ggcactgatt
     4801 gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt taaacgccat tcccgaattt
     4861 gtgacccgag gactacagat tgctggcggt tttatcgtgg tggtcggtta cgccatggtg
     4921 ctacgtatga tgggcgtgaa atatttgatg cctttctttt tcctcggttt cctcgcaggt
     4981 ggctacctcg atctcagcct gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc
     5041 tacatccagc taaatccaca gtggcgtaaa gctgaacctc aactccaggc caccgcctcc
     5101 accgcccttg accaacttga cgattaacgg agccatcatg gaacagagaa aaattacacg
     5161 cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa caggcgtcct ttaactttga
     5221 acgtattcac gggctgggct tttgctacga catgatcccc gccatcaagc gactttaccc
     5281 attaaaagag gatcaggttg cggcgctcag gctacaccta gtgttcttca ataccacgcc
     5341 agccgtatgt ggcccggtca tcggcgcgga aattgatgac ggtaccatca acggcatcaa
     5401 agtcggtctg atgggaccat tggcaggagt tggcgatcca ctggtctggg gaacgctgcg
     5461 cccgattacc gccgcgctcg gcgcatctct ggcactttcg ggcaacattc tcggcccgct
     5521 gctgttcttc tttattttca acgcggtgcg tctggcgatg aagtggtatg gcctacagct
     5581 cggctttcgc aaaggggtga atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact
     5641 caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc gtgctggtga ccaaatggac
     5701 gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc gccgatggct ccaccgtcac
     5761 catgaccgtg cagaacattc tcgaccaact ttgccctggt ttgctggcgc tcggtctgac
     5821 gctactaatg gttcgtctgc tcaacaaaaa aattaacccg gtatggctga ttttcgccct
     5881 gtttggctta gggattatcg gcaatgctct gggcttcctg tcctgattct tcgccccggc
     5941 acgactgccg aggccatcgc tcaacatgag gtggtttatg aaaacaacag ctctgcgtct
     6001 ttatggtaaa cgtgatttac gcctggaaac ctttgacctt cctgaaatgc aggaggatga
     6061 aatcctcgca acggtggtca ctgacagcct gtgcctctct tcctggaaag aggccaatct
     6121 gggtgaaaac cataaaaaag tacccgacga tgtgacgacc aacccaatca tcatcggcca
     6181 cgaattttgc ggcgatattc tggccgtggg taaaaagtgg cagcacaaat tccagccggg
     6241 tcagcgttat gtgattcagg ccaacctgca actccccgac cgcccggatt gccccggcta
     6301 ctccttcccg tgggtagacg gcgaagcgac gcatgtggtt attcccaacg aggtcatgga
     6361 acaatattgc ctgctggcat acgacggcga aacctatttt gaaggctcac tggttgaacc
     6421 gctttcctgc gtgattggcg cgttcaacgc caactatcat cttcaggaag gtagttataa
     6481 ccacacgatg gggattcgcc cgcaagggcg cacgctgatc ctcggcggca ccggaccaat
     6541 gggactgttg gcgattgatt atgcgctgca tggaccagtt aacccgtcgc tgctcgtcat
     6601 taccgatacc gacaacgaca aattgagtta tgcgcgcaag cactatccat cagaaccgca
     6661 aacactgatt cattatctca atgccaccga tgcagcattt gatacgttaa tggcgctgag
     6721 tggcggtcac ggcttcgatg atattttcgt ctttgtgcct aatgaaggac tggtgactct
     6781 cgcctcttcc ttgctggcga cagatggttg cctgaatttc ttcgccggac cgcaggataa
     6841 acatttcagc gcgccaatta atttctacga tgtgcattat gcatttaccc actacgtggg
     6901 cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc aaactgatcg aagagaaaaa
     6961 agtgcaggcc gcaaaagtgg taacacatat tcttgggctg aatgccgcgg gcgaaaccac
     7021 gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg tataccggga aatacctgcc
     7081 gctgacgtca ctcacgcaga ttcaggatca agcactggcg gcgattctgg cgcgtcatca
     7141 ggggatctgg tccggtgagg cggagcaata cctgctcgcc catgcagagg caatttccca
     7201 tgattaatcg cgctacttaa ctctgcatgt cgctggctgg tcgccccagc aacttcgcga
     7261 ccggatgtgc ggctgattgc tgaagcagca caatttgcac gagaaggata aaaaatatca
     7321 gcgccttaag gggcgctcaa attggtggca aagtacacat tgtttatgcc ggatgcggaa
     7381 tgaagcctta ttcggtctac ataattttga aaatccaata tattgcagtt atctggtagg
     7441 ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca tcagtctcag cgcccggtgc
     7501 aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcatgcctt
     7561 ccgtgatttc ttactaaccg gagaagtaat ggaactgctt ttattgagta actcgacgct
     7621 gccgggtaaa gcctggctgg aacatgcact gccgctaatt gctgaacagt tgcagggtcg
     7681 ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag acctgggatg attacacaga
     7741 gaaaacggct gcggttctcg ctccgctggg tgtttctgtc accggtattc atagcgttgt
     7801 cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt gtcggcggcg gtaatacttt
     7861 ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct ccaattactg acgtggttaa
     7921 acgtggcgcg ctgtatattg gctggagcgc aggcgctaac cttgcttgcc caaccattcg
     7981 taccaccaac gatatgccga ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc
     8041 gttgcaaatc aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg
     8101 tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa ctgacgatta ttggtctgcc
     8161 ggaaggtaac tggatcacag tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac
     8221 ttatgtgttt aaggctggtg aagaagcggt tccgctggaa gctggtcacc gtttttaagt
     8281 gaattgccgg atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg
     8341 tcttatcagg cctacaggat tcacaatatt aatactcgtc ccgtccttca tcttcatccg
     8401 gctgttccag cacactgtaa gccaccgaac aaaacagcga gttcaggcgc tgcatatctc
     8461 ccagtaagcc taaatgtagc gaactggttt caatgctctg tacgttttgc tgatgcaggc
     8521 gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg aaaacgatgt ttgctgcgac
     8581 gcaaacgacg agcgctggtg acatcgccag agaagaacac cgacattgcc agctttaaat
     8641 tactgagcaa ttgctcatag agcgcatcca gttccttcaa cccatcaagc gaaaatgccc
     8701 gccgtgctgc cagcgattta tcggcaattt cgctgcccat gcgctcgacg atatcggagg
     8761 cctgttcaag gttgagcgac atttcgatga tctccgccca gcggcgcgac tcttcctccg
     8821 ccaactcttc ttttggcatc cgcgccagat acagcttaat ggcggtgtag agaacgttga
     8881 tatcatctgc cagcttacgc agctctttct cctgccgtgg ctcgccgtgc atcactttat
     8941 tcagcccttc catcatctgt tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt
     9001 tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc cagatgttta ggccgtagct
     9061 gggtatccag ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa
     9121 caaatggcag catgaccagg caacgtacaa ggttgtagaa gacgtggaaa tagatcacca
     9181 gttccgcttt tggcaatgac agcttcccca ttgtctctgc cagcaaatgg acaaacggca
     9241 ggataatcag gctacccacc agcttaaaca gcagactacc cagcgcgaca cggcgggctg
     9301 cggcattggc ggcactgttg ttgagcatcg ccagcaggcc ggaaccgagg ttagctccaa
     9361 tcaccagaca gagcgccacg gggaaggaga taatgcctgc agcggtcaga gtcgcggtca
     9421 gcagtacagc agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat
     9481 ccagcagaat atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga
     9541 tcggcgttac ggcctgcaca atcaactcca gcgccagcaa aatcagccca agaccaatac
     9601 cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga
     9661 taaaaataag taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg
     9721 ccgtcccgac atctgccccc agcacaatga ccagagccgg tgcgagggct accagatcct
     9781 gagcgacgaa cgaggtcacc agcatggtgg tggcattact gctctgtacc agtgcggtaa
     9841 cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac gctccggcta aggacagtac
     9901 gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac aatatgagtc ccccagacca
     9961 gcagggcgac ggcagaaagc aggtgaagca gcgttaacac gagttcaggt tctccttatc
    10021 gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg aaacacgctg actggcatct
    10081 ttactgtgcc ttttgacatt cgtttttccg gctaagatta ttccagcctg tttccagtat
    10141 aagtgtttca tattaataaa gagacagggc actcattaag cgccctgttt gttgtaagga
    10201 aaagtgacag atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct
    10261 caacatcgga gacgctcata cctttacggc gggcatagtc ttcaacctga tcgcgctgaa
    10321 tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa ataccaaccc gaaaccgatg
    10381 caccaggcca catcgcgaaa gattctgtga gtttcatgcc agtatgtttt tccacttcca
    10441 gcagctccca gatggtggct ttttccgtat gttccgggca ggccggatag cccggtgccg
    10501 gacggatgcc ctggtagttt tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg
    10561 catagcccca gtagacttta cgcacacgct catggagata ctccgcaaag gcttcggcta
    10621 aacggtcggc aagcgctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg
    10681 catcagccag tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg
    10741 ctttaccaga aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aatcccgttt
    10801 tttcggtctg ttgacgcaga tggtggctga cgttgatcac atgggtgcgc gtttcgtcac
    10861 ggtagatttc aatgtcatcg cccacacggt ttgccgggaa caggcccacc acgccacgcg
    10921 gattcagcgt tttctcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc
    10981 gctgcgcctc aacgcccacc acttcatctt ccagaatgcg cggatacttc ccggccagcg
    11041 accaggtcat aaagaacggt gtccagtcga tgtaattacg cagcgtttcg atgctggctt
    11101 ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaatg
    11161 cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg tgtgcgtggt ttcttgcgcc
    11221 cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg agcgacaaaa tcatcacgct
    11281 gagtatcgga aagcagcgcc gctaccacac caacggtgcg cgaggcattc tgcacataca
    11341 ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt gtgcgctttt gaggtggtcg
    11401 cgccgccaat cagtaacgga atggcgaagc cctgacgctc catctctttc gccacgttaa
    11461 ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc aatcaaatca gcattcactt
    11521 ctttggcgat acggagaatt ttttccgcag gcaccataac gccgagatcg acaatttcgt
    11581 agttgttaca ttgcagcacc acaccaacga tatttttacc gatgtcgtgg acatcgcctt
    11641 tcacggtggc gatcaccatc ttgccgttgg ttttaccctg ctctttgctg gcttcaataa
    11701 acggttcgag atacgccact gcctgtttca tgacgcgcgc cgatttgacc acctgtggca
    11761 ggaacatttt gccttcgcca aacagatcgc cgaccacgtt catgccgtcc atcaacgggc
    11821 cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc ttcttcagta tcctgctcga
    11881 taaactcggt tatgcctttg accagcgagt attccagacg tttattcacg tcccacgagc
    11941 gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt actgccgcga tatttctcgg
    12001 caagctccag taaacgctca gtgccatcgt cacgacgatt aagaatcaca tcttccaccg
    12061 cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag ttgcccggcg ttgacgatcc
    12121 ccatatccat gccattgcga atagcgtagt agaggaacac tgcgtgaatg gcttcgcgca
    12181 ccggatcgtt gccacggaat gagaaagaaa cgttagatac gccgccggaa atcagcgcgt
    12241 gcggcagttc gcgtttgatg tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt
    12301 gctcttcaat accggtggcg accgcgaaga tgttcgggtc gaagatgata tcttctggcg
    12361 ggaagccaac ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgtg
    12421 cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac caccactgcc gcaccgtagc
    12481 gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc ctctttcatc gagatagagt
    12541 taacaatgcc tttgccctgg atacacttca gacctttttc aatgacatcc cattttgagg
    12601 agtcgatcat aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac
    12661 gcaccatcgc cgcttcggca tcgagcatcc cttcatccat gttgatatcg ataatctgcg
    12721 cgccgttttc cacctgctga cgcgccacat ccagcgcctc gctgtatttc tcttctttga
    12781 tcaggcgctt gaacttagca gagccggtga cgttggtgcg ttcacccacg ttcacaaaca
    12841 ggctatcgtc gccaatgttc agcggctcca ggccagacaa acggcaggct acgggaattt
    12901 ccggcagttt gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgcg
    12961 gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc cgcttgcgcc cattcacgta
    13021 tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc aaaggcgtta ggcagcccgg
    13081 cgttcgggtg ggcggtgacg tagcattccg caatccgtga tagttcctgc acgtactggc
    13141 gcagttcatc gggccccagc gcacagttca ggccaaaggt cagcgcttcg gcgtggcgca
    13201 atgagttgta aaatgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga
    13261 tggtgccgga gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg
    13321 caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg
    13381 caccaccttc caccagcgct ttggtggatt cgcgataggc cgccgccagc tggtcaaaag
    13441 tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat agacgccgtg cggttggttg
    13501 ggccaagaac accggcaacg tagcgcggtt tctccggcgt gcgcgcggtc cactcgtcag
    13561 cacaggcgcg cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct
    13621 ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc
    13681 ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc cggtttactg agtaccagca
    13741 ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg
    13801 cttcgttcag tcgatagctc tggatcatgg tgcccatacc gccgtccaac accagaatac
    13861 gttcatttaa ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc
    13921 tcaacccaga tttaataaaa attcaacaaa ccatactgga ataaacgcat ctgtggtaaa
    13981 agcgaccacc acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac
    14041 tattgcatta gctaacaata aaaatgaaaa taatttccac gatacagaaa aaggaggctg
    14101 tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag
    14161 cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat
    14221 ccaatggcag cgtggcactc acagagctgt cgcaacaagc cgggttaccc aattccacga
    14281 cccaccgcct gctaaccacg atgcaacagc agggtttcgt gcgtcaggtc ggcgaactgg
    14341 gacactgggc aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta
    14401 atttgttagc aattgttcac cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg
    14461 tcaatatggc ggtgcttgat caaagcgatc acgaagcgat tattatcgac caggtacagt
    14521 gtacccacct gatgcgaatg tccgcgccta tcggcggtaa attgccgatg cacgcttcag
    14581 gtgcgggtaa agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc
    14641 gcaaagggtt acatgcctat acccacgcaa ctctggtgtc tcctgtgcat ttaaaagaag
    14701 atctcgccca aacgcgcaaa cggggttatt catttgacga tgaggaacat gcgctggggc
    14761 tacgttgcct tgcagcgtgt attttcgatg agcaccgcga accgtttgcc gcaatttcta
    14821 tttccggacc gatttcacgt attaccgatg atcgcgtgac cgagtttggc gcgatggtga
    14881 ttaaagcggc gaaggaagtg acgctggcgt acggtggaat gcgctgactt tttctggcgg
    14941 gcagaggcaa tattctgccc atcatacctg agtggcaata gaataagggt gtctgttaat
    15001 cgcattgacg ccaaaataac ttaatgtcat acacttcaca cacttttatt aactcagcat
    15061 tatttttaaa catcaaacca cttaattatt aacaaa
//
LOCUS       DBKELA010000095        15034 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.095, whole genome shotgun sequence.
ACCESSION   DBKELA010000095 DBKELA010000000
VERSION     DBKELA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.095"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..176)
                     /locus_tag="ACUQR0_003090"
     CDS             complement(<1..176)
                     /locus_tag="ACUQR0_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110032.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="ACUQR0_003091"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="ACUQR0_003091"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HHV9110033.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSSDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="ACUQR0_003092"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="ACUQR0_003092"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HHV9110034.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="ACUQR0_003093"
     CDS             complement(2012..2884)
                     /locus_tag="ACUQR0_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HHV9110035.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="ACUQR0_003094"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="ACUQR0_003094"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HHV9110036.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="ACUQR0_003095"
     CDS             complement(3576..4361)
                     /locus_tag="ACUQR0_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHV9110037.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="ACUQR0_003096"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="ACUQR0_003096"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HHV9110038.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="ACUQR0_003097"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="ACUQR0_003097"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HHV9110039.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="ACUQR0_003098"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="ACUQR0_003098"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HHV9110040.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="ACUQR0_003099"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="ACUQR0_003099"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HHV9110041.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8653..9555
                     /locus_tag="ACUQR0_003100"
                     /pseudo
     CDS             8653..9555
                     /locus_tag="ACUQR0_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="ACUQR0_003101"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="ACUQR0_003101"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HHV9110042.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11836..13206
                     /locus_tag="ACUQR0_003102"
                     /pseudo
     CDS             11836..13206
                     /locus_tag="ACUQR0_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13249..14652
                     /locus_tag="ACUQR0_003103"
     CDS             13249..14652
                     /locus_tag="ACUQR0_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9110043.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14720..>15034
                     /locus_tag="ACUQR0_003104"
     CDS             14720..>15034
                     /locus_tag="ACUQR0_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HHV9110044.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGA"
BASE COUNT         3695 a         3869 c         3740 g         3730 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gctgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 agcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctgt gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc
    12301 tcactggcgg catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga
    12361 ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc
    12421 acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag
    12481 cgttatgtcg aaacccaacc aatccggcgc aaaagcctgg cctgttgcaa tctttccgcg
    12541 caattgccgg taaccgccca ctgttcattc tgtgtattgc caacctctgc accttagggg
    12601 cgtttaacgt caagctcgcc attcaggtct attacaccca gtacgtactt aacgatccca
    12661 tcctgttgtc gtatatggga tttttcagca tgggctgtat tttcatcggc gtgttcctga
    12721 tgcctggcgc agtcagacgt tttggtaaga agaaggtcta tatcggcggc ctgctgattt
    12781 gggtgctggg cgatctgctc aactatttct tcggcggcgg ctcggtcagc tttgtggcgt
    12841 tctcctgcct ggcgttcttc ggctcagcgc ctgaactggg cgctggtttc cgacaccgtc
    12901 gagtacggcg agtggcgcac cggcgtgcgt tcggaaggca cggtctacac cggcttcacc
    12961 ttctttcgca aagtgtctca ggcgctggct ggtttcttcc ccggctggat gctgacgcaa
    13021 attggctatg tgccgaacgt cgcacaggct gaccacacca ttgaagggtt acgccagttg
    13081 atcttcatct acccaagcgc actggcggta gtcaccattg tggcgatggg ttgcttctac
    13141 agcctgaacg agaagatgta tgtccgcatt gtggaagaga tagaagcccg taaacgcacg
    13201 gcgtaataat aataaacgac gccctgcggg gcgttataag gagtgattat gtctgaccat
    13261 aatccactga cattaaaact gaatctgcgg gaaaaaatcg cctatggtat gggcgacgtc
    13321 ggttcgaatt taatgctctg catcggtact ctgtatctcc tcaaacttta taccgatgaa
    13381 ttagggatgc ctgcttacta tggcgggatt atctttctgg tcgcgaagtt ttttaccgcg
    13441 tttaccgata tgctcaccgg atttttactc gactcgcgga aaaatattgg gcctaaaggc
    13501 aaatttcgtc cctttatttt atatgcagca gttccggcgg cgttaattgc aacgctccag
    13561 tttatcgcca ccaccttttg tttgccggtt aaaacgacga ttgccaccac gctgtttatg
    13621 atgtttggac tttcatacag tctgatgaac tgctcgtatg gtgcgatgat cccggcaatt
    13681 accaaaaacc cgaatgagcg cgcgcagctc gcggcttacc gccagggtgg tgcaaccata
    13741 gggctattga tttgtaccgt ggcgtttatt ccgttgcagt cgcttttttc tgactcaacc
    13801 gtcggttatg cctgtgcggc acttatgttc tccattggcg gctttatttt tatgatgctg
    13861 tgctacagag gcgtcaaaga gcattatgtg gacacagcgc caaccggaca taaagccagt
    13921 attctcaaat ctttttgcgc gatatttcgt aatccaccat tgctggtttt atgcattgct
    13981 aacctgtgta ctctggcggc atttaatatc aaactggcga ttcaggtcta ttacacccag
    14041 tatgtgctga atgatattaa tttattgtcg tggatgggat tcttcagtat gggatgcatc
    14101 ctcgtcggcg tattactggt gccagtaact gtaaaatgtt ttggtaaaaa acaggtttat
    14161 ttagctggca tggtgctatg ggcggtgggt gatatactga attatttctg gggaagtaac
    14221 tctttcactt tcgtcatgtt ctcttgtgtc gccttttttg gcacggcctt tgtaaacagc
    14281 ctgaactggg cactggtacc agataccgta gattacggtg aatggaaaac cggtattcgt
    14341 gccgaaggtt ctgtttatac cggttatacc ttctttcgta aaatttctgc cgcacttgct
    14401 ggcttcttgc caggcattat gctgacgcaa attggttatg ttcccaacat cgcgcaaagc
    14461 gatgcgacat tacagggttt gcgtcagctc atttttatct ggccttgtgc actggcaatt
    14521 attgctgcat tgacgatggg attcttttac acactcaatg aaaaacggtt tgcattgatt
    14581 attgaggaaa tcaaccaacg caaaaataaa gagatggcga cagaaaaaaa aacggcttcc
    14641 gtaacgttat aaatgtcaat agccgctatt tccatcctgg tgggtggcgg cctccctacg
    14701 tttaaaaaat ggacttatta tgaaaaagat taatgcaata attttattat cttctctgac
    14761 ctctgcctcg gtatttgctg gcgcatacgt cgaaaacagg gaggcatata atcttgcctc
    14821 cgatcagggc gaggttatgc tacgtgtggg ttataacttc gatatgggcg cgggtattat
    14881 gttaaccaat acctacacct ttcagcgaga agatgaacta aaacatggct ataatgaaat
    14941 tgaaggttgg tatcctttat tcaaaccaac cgataaatta accatccagc ccggtggctt
    15001 aattaatgat aagagtatca gttcgggtgg tgca
//
LOCUS       DBKELA010000096        15030 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.096, whole genome shotgun sequence.
ACCESSION   DBKELA010000096 DBKELA010000000
VERSION     DBKELA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.096"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..236
                     /locus_tag="ACUQR0_003105"
     CDS             <1..236
                     /locus_tag="ACUQR0_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HHV9110045.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="ACUQR0_003106"
     CDS             246..884
                     /locus_tag="ACUQR0_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HHV9110046.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="ACUQR0_003107"
     CDS             887..2050
                     /locus_tag="ACUQR0_003107"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HHV9110047.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3759
                     /locus_tag="ACUQR0_003108"
     CDS             2134..3759
                     /locus_tag="ACUQR0_003108"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HHV9110048.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="ACUQR0_003109"
     CDS             complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="ACUQR0_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HHV9110049.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="ACUQR0_003110"
     CDS             complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="ACUQR0_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HHV9110050.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4811..5560
                     /gene="yjfP"
                     /locus_tag="ACUQR0_003111"
     CDS             4811..5560
                     /gene="yjfP"
                     /locus_tag="ACUQR0_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HHV9110051.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="ACUQR0_003112"
     CDS             complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="ACUQR0_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HHV9110052.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="ACUQR0_003113"
     CDS             complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="ACUQR0_003113"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HHV9110053.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7839..9236
                     /gene="ulaA"
                     /locus_tag="ACUQR0_003114"
     CDS             7839..9236
                     /gene="ulaA"
                     /locus_tag="ACUQR0_003114"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HHV9110054.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9252..9557
                     /gene="ulaB"
                     /locus_tag="ACUQR0_003115"
     CDS             9252..9557
                     /gene="ulaB"
                     /locus_tag="ACUQR0_003115"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HHV9110055.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9567..10031
                     /gene="ulaC"
                     /locus_tag="ACUQR0_003116"
     CDS             9567..10031
                     /gene="ulaC"
                     /locus_tag="ACUQR0_003116"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HHV9110056.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10045..10695
                     /gene="ulaD"
                     /locus_tag="ACUQR0_003117"
     CDS             10045..10695
                     /gene="ulaD"
                     /locus_tag="ACUQR0_003117"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HHV9110057.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10705..11541
                     /gene="ulaE"
                     /locus_tag="ACUQR0_003118"
     CDS             10705..11541
                     /gene="ulaE"
                     /locus_tag="ACUQR0_003118"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HHV9110058.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSRERRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11541..12227
                     /gene="ulaF"
                     /locus_tag="ACUQR0_003119"
     CDS             11541..12227
                     /gene="ulaF"
                     /locus_tag="ACUQR0_003119"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HHV9110059.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12356..12631)
                     /gene="yjfY"
                     /locus_tag="ACUQR0_003120"
     CDS             complement(12356..12631)
                     /gene="yjfY"
                     /locus_tag="ACUQR0_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HHV9110060.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12958..13353
                     /gene="rpsF"
                     /locus_tag="ACUQR0_003121"
     CDS             12958..13353
                     /gene="rpsF"
                     /locus_tag="ACUQR0_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HHV9110061.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13360..13674
                     /gene="priB"
                     /locus_tag="ACUQR0_003122"
     CDS             13360..13674
                     /gene="priB"
                     /locus_tag="ACUQR0_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HHV9110062.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13679..13906
                     /gene="rpsR"
                     /locus_tag="ACUQR0_003123"
     CDS             13679..13906
                     /gene="rpsR"
                     /locus_tag="ACUQR0_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HHV9110063.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13948..14397
                     /gene="rplI"
                     /locus_tag="ACUQR0_003124"
     CDS             13948..14397
                     /gene="rplI"
                     /locus_tag="ACUQR0_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HHV9110064.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14468..>15030)
                     /locus_tag="ACUQR0_003125"
     CDS             complement(14468..>15030)
                     /locus_tag="ACUQR0_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HHV9110065.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3510 a         3667 c         4051 g         3802 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cattttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatgcgca ggttgaggct
     2521 gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt acagatgtta
     2581 cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga ttctcactta
     2641 ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga aaagcagggc
     2701 ggttccgatg tcatgagcaa caccacccgt gcagagcgtt tggaagatgg ctcttatcgg
     2761 ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct ggtgctggcg
     2821 cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga cgggcaacgc
     2881 aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa cgccagttgc
     2941 gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga aggaattcgt
     3001 ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctgggtag ccatgccatg
     3061 atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt ttttggtaat
     3121 ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa
     3181 gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc cgatgccaaa
     3241 gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg caaacgaggt
     3301 atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg cgaggagagc
     3361 gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg ttccggcaat
     3421 attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata cgacttattg
     3481 tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt
     3541 ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac ccatcagcta
     3601 ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg
     3661 tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca gatccagaat
     3721 gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac tgatgcgacg
     3781 ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc
     3841 cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat cgcctgtacg
     3901 cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca tgacgcacct
     3961 tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc tgaaatattg
     4021 ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact ggcgaattct
     4081 gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt
     4141 aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc attccatcgc
     4201 ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa cgcataggga
     4261 cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt acaaaatggc
     4321 ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa cgtaataatc
     4381 agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg gagaaccccg
     4441 aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc ggcgaatctc
     4501 ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc
     4561 acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat tacgtttcct
     4621 gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc gaattgttac
     4681 ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct caacattatg
     4741 aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac cagacaaaaa
     4801 ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg ttcttcatgc
     4861 ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc acggctttac
     4921 ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt
     4981 gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag cgcggcggtt
     5041 aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt tacgtgcggc
     5101 aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg
     5161 cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat
     5221 gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc tgatacctga
     5281 aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag agtgggaagc
     5341 gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg gcctcgacga
     5401 tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg agacgggacg
     5461 ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgtatta cgcctgaggc
     5521 gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa tgctgacccc
     5581 ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga tcagcatatc
     5641 gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac
     5701 cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg ctgtcagcat
     5761 atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca tccagtgccc
     5821 ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac ttttgttgta
     5881 ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg
     5941 tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa gcagaaacgc
     6001 ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg aggccgcttt
     6061 agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg gcgtccagcg
     6121 cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt tgccgctttc
     6181 gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac
     6241 tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt gatgtctttg
     6301 tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat ttgaaatagc
     6361 caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata aacgacagat
     6421 tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc gaaaccgcgc
     6481 ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc gccaacctgc
     6541 cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca cagcacgcgg
     6601 atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg gataaccact
     6661 ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt gtcggtaata
     6721 ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc gttaccgtgc
     6781 ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc gccaggggtt
     6841 ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc agccgctttt
     6901 tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca
     6961 atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc tttcggtacg
     7021 ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca
     7081 cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc
     7141 gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt
     7201 aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc ctgtttcatt
     7261 aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca aacgttggtg
     7321 ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc aaatgtgcca
     7381 ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc cgggaaagtg
     7441 ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat ttaccttcat
     7501 gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac tattccttca
     7561 ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat tttgagtgtg
     7621 gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa ataattgttt
     7681 cgttgtgtga atcacactct atccgtgtgg aattatttgc gggtcgcgcc acatttaatc
     7741 ataaataatc ttgttgtgat tacttttgaa aattagagtg agtacacaac attccgggtg
     7801 tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
     7861 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
     7921 gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa
     7981 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc accttcaaac
     8041 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
     8101 cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta ggttacgcgg
     8161 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
     8221 gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt gccgttacgt
     8281 tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt ctggtttcgc
     8341 tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg acggacggct
     8401 gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat aaagttgcgc
     8461 cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc tggctgaata
     8521 ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt ggtgccattc
     8581 tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac tggacggtct
     8641 atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact cagggtgtgc
     8701 gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc ctgatccctg
     8761 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac gccgtggtct
     8821 ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg gtcgcctgcg
     8881 gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac gccaccatcg
     8941 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt ctggtgatgg
     9001 ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg agtgcctgga
     9061 tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     9121 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctggcc
     9181 gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct gcttaataag
     9241 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca
     9301 tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa
     9361 atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     9421 ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt ggcgtgcgca
     9481 acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa gcgcatttcc
     9541 cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg gagaataaat
     9601 ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc
     9661 tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg
     9721 aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg
     9781 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     9841 accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc gatgccaaca
     9901 cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg
     9961 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
    10021 cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac
    10081 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
    10141 gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg
    10201 ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt
    10261 tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc
    10321 aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa
    10381 ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag
    10441 gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat
    10501 atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg
    10561 gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc
    10621 agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
    10681 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa
    10741 aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc
    10801 ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg cctcgactgg
    10861 agccgcgaac ggcgtctggc gctggtgaat gctattgttg aaaccggcgt gcgtgtgccg
    10921 tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg
    10981 gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc
    11041 gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
    11101 cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca ggtgacgctg
    11161 gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg
    11221 cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
    11281 gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg
    11341 aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc
    11401 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
    11461 tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat ggccaaagca
    11521 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg
    11581 atctgccgcg ctatgtgctg gtgaccttta cttggggcaa cgtcagcgct atcgaccgcg
    11641 aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg aaagcagacg
    11701 atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg
    11761 acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata
    11821 cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg gcattaggca
    11881 ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta agcgaagaag
    11941 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
    12001 ccgagccgct gcatacgccg gaaattgtgg tgtatcagca cggaccgttc gcctggggga
    12061 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
    12121 ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg atgaataaac
    12181 acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
    12241 gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
    12301 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
    12361 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
    12421 cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc
    12481 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
    12541 ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca
    12601 cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct gactttgtgt
    12661 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
    12721 accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga acttcaaatc
    12781 agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc ttgagtttag
    12841 cggggtatgc cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
    12901 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
    12961 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
    13021 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
    13081 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
    13141 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
    13201 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
    13261 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
    13321 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
    13381 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
    13441 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
    13501 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
    13561 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
    13621 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
    13681 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
    13741 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
    13801 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
    13861 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
    13921 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
    13981 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
    14041 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
    14101 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
    14161 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
    14221 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
    14281 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
    14341 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
    14401 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
    14461 tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
    14521 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
    14581 cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga ccaattttca
    14641 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
    14701 tagaaacgcc tgtaacagtg tgaagttggt cgatataaag atcgattgtt ggtagttcag
    14761 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
    14821 tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg tatttactgt
    14881 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
    14941 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc
    15001 atccaaaaga attatcacca ttcattgtta
//
LOCUS       DBKELA010000097        14903 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.097, whole genome shotgun sequence.
ACCESSION   DBKELA010000097 DBKELA010000000
VERSION     DBKELA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.097"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(92..517)
                     /gene="arsC"
                     /locus_tag="ACUQR0_003126"
     CDS             complement(92..517)
                     /gene="arsC"
                     /locus_tag="ACUQR0_003126"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HHV9110066.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(530..1819)
                     /gene="arsB"
                     /locus_tag="ACUQR0_003127"
     CDS             complement(530..1819)
                     /gene="arsB"
                     /locus_tag="ACUQR0_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HHV9110067.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1873..2226)
                     /gene="arsR"
                     /locus_tag="ACUQR0_003128"
     CDS             complement(1873..2226)
                     /gene="arsR"
                     /locus_tag="ACUQR0_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HHV9110068.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2970..3053
                     /gene="dinQ"
                     /locus_tag="ACUQR0_003129"
     CDS             2970..3053
                     /gene="dinQ"
                     /locus_tag="ACUQR0_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HHV9110069.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3107..4459)
                     /gene="gorA"
                     /locus_tag="ACUQR0_003130"
     CDS             complement(3107..4459)
                     /gene="gorA"
                     /locus_tag="ACUQR0_003130"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HHV9110070.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4531..5373)
                     /gene="rlmJ"
                     /locus_tag="ACUQR0_003131"
     CDS             complement(4531..5373)
                     /gene="rlmJ"
                     /locus_tag="ACUQR0_003131"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HHV9110071.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5576..7618
                     /gene="prlC"
                     /locus_tag="ACUQR0_003132"
     CDS             5576..7618
                     /gene="prlC"
                     /locus_tag="ACUQR0_003132"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HHV9110072.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7626..8378
                     /gene="rsmJ"
                     /locus_tag="ACUQR0_003133"
     CDS             7626..8378
                     /gene="rsmJ"
                     /locus_tag="ACUQR0_003133"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HHV9110073.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8427..9896)
                     /gene="dtpB"
                     /locus_tag="ACUQR0_003134"
                     /pseudo
     CDS             complement(8427..9896)
                     /gene="dtpB"
                     /locus_tag="ACUQR0_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            10062..10115
                     /locus_tag="ACUQR0_003135"
     CDS             10062..10115
                     /locus_tag="ACUQR0_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110074.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(10214..10648)
                     /gene="uspA"
                     /locus_tag="ACUQR0_003136"
     CDS             complement(10214..10648)
                     /gene="uspA"
                     /locus_tag="ACUQR0_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HHV9110075.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11042..11377
                     /gene="uspB"
                     /locus_tag="ACUQR0_003137"
     CDS             11042..11377
                     /gene="uspB"
                     /locus_tag="ACUQR0_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HHV9110076.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11448..12947)
                     /gene="pitA"
                     /locus_tag="ACUQR0_003138"
     CDS             complement(11448..12947)
                     /gene="pitA"
                     /locus_tag="ACUQR0_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HHV9110077.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13179..14378
                     /locus_tag="ACUQR0_003139"
     CDS             13179..14378
                     /locus_tag="ACUQR0_003139"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHV9110078.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3711 a         3786 c         3921 g         3485 t
ORIGIN      
        1 acggttttgt aggtcggata agacgcgtta gcgtcgcatc cgacataaac aaagcgcact
       61 ttaccaacta tcggcagtgg atatcgccgc tttatttcag gcgcttaccc gcttcatcaa
      121 ccactttctc gccatcttcc ttggagaatg cgcctttttg cgcatctggc agaatttcca
      181 gcaccacttc tgaagggcgg cacaggcgag ttcccagcgg cgtcaccaca atcgggcgat
      241 taatcagaat cgggtgctga agcataaagt cgattaaccg atcgtcagta aatttatctt
      301 ccgcaaggcc cagctcctca tacggttcga cgtttttacg cagcagcgcg cgtacggaaa
      361 tccccatatc ggcaatgagt ttgaccagtt catcgcgcgt tggcggagtt tccagataat
      421 ggataatggt cggttctgtg ccgctgttgc ggatcatctc cagcgtatta cgcgacgtgc
      481 cgcaggccgg gttgtgataa atggtaatgt tgctcatatc agtatctcat tacaaagtga
      541 aagagagacg tagcgccagc gcagccagcg tcacaaacag cacaggcagg gtcatgataa
      601 tccctgtacg gaagtaatat ccccagctga tagtcatatt cttctgcgaa agtacgtgca
      661 gccagagcag cgttgccagg ctaccaatcg gggtaatttt cggccccaaa tcgcagccaa
      721 tcacgttggc ataaaccatc gcttctttga taacgccaga tgccgtgctg ccatcaatgg
      781 acaacgcgcc aaccagtacc gtcggcatat tgttcataat agaagagagg aaggcggtga
      841 ggaatccggt gccgagcgtc gcggcccaca ggccgttatc cgccagcacg ttgagtacgc
      901 cagaaagata ttccgttaat ccggcattgc gcaggccata aaccaccaga tacatgccga
      961 gcgagaagat gacaatctgc cagggggcac cgcgcaggac tttacccgta ttaatcgcat
     1021 gaccgcgttt agcgacgaca aataatatca gtgcgcccac agctgcaatg gcgctcaccg
     1081 gaatgccgag cggttccaag acgaaaaatc ccaccagcag aagcagtaaa acaacccagc
     1141 cagttttgaa cgtagcagga tctttgatcg cttctgcggg agatttcagc agcgccatat
     1201 cgtagttctg cggaatatct ttgcgaaaat agagatgtag catcaccagc gtggcaacaa
     1261 tcgcggcgat atccaccggc accatcaccg aggcgtattc gcgaaagccg aggccaaaga
     1321 aatcagcgga aacgatattc accaggttgg agacaataag cggcaggctg gcggtatcgg
     1381 caatgaatcc ggccgccatc acgaacgcca gcgtcgtgcc tttactgaat cctaaagcca
     1441 gcagcatggc gatgacaatc ggcgtcaaaa taagcgccgc gccatcattg gcaaacaggg
     1501 ccgcaacggc ggcaccgagc aggacaatcc aggtaaacag caagcgaccg cgtccattgc
     1561 cccagcgtga aacgtgcagc gctgcccatt caaaaaagcc ggactcatcc agcagcaggc
     1621 tgatgataat gacggcaata aacgccgccg tcgcgttcca gacgatattc cacaccaccg
     1681 gaatatcacc cggatgaacc acgcccgtaa ccaacgccag taccgcgccg agcgttgcac
     1741 tccagccgat gcctaaacct ttcggctgcc agataaccaa tacgatggtc aggacaaaga
     1801 tagcgcctgc cagtaacata atgcctcccg gataaaacac atgtgcaaat ccatatgtgt
     1861 ttagctaaat ttttaactgc aaatgttctt actgtctccg gaacagtttt gtctggccag
     1921 gttgcggaca attgcctgaa tcttttcctg ttcacatcgc caggcttcat caataatttt
     1981 cgccgcccat gctggaatat gcggcgataa gcggtaatga acccacttac cttgcttgcg
     2041 gtccagcaat agcccgcttt cacgcagcaa tgccagatgg cgggatatct tgggctgcga
     2101 ctgatcgagg gcagtgcaga gatcgcagac gcataactct cccagttcgc taagcagtaa
     2161 aacgatgccc agacgggttt catcagcaag aattttgaac aattggatgg gtaacagaaa
     2221 tgacatattg cgctcctgat tgttgcaggt agtgtctctc ttcgaagcgg ataagtcaaa
     2281 aacatatatg acttaacgaa tgtataagtg cagaggaagg taataggtgt gaattttgag
     2341 ttggctattc atttgaaagg cggtttgaat caggacgtaa aaaaaccgac tttgcgtcgg
     2401 tttttttact cttcagcctg agttggtggc tctggctgga gtgagaatgc ccacatctaa
     2461 cggttcaata ctattagcca gtggctaaca ttgcaagcgt tagcgatttc ttcagctgga
     2521 gaagggacag tttgctatac atggtggtga aagaggattt atatactttc atcgggtcgc
     2581 tgccgtacca agccttgtcc gaatccggac gtaaaaaaac cgacttcgcg tcggtttttt
     2641 tactcttcag cccagagttg gtggctctgg ctggagtgag aatgccatca tctaacgcat
     2701 caatgctaat accatactga aactattgca aggttatgct ggttatttag cctaattaca
     2761 ctgtatgatt atccagttag ctctgaggca ttttcactct ggcaatgcgc ataaacgctt
     2821 tcaaagtcct ggtcagaagt acgggtggtg ccgttaactg atgctctggc cggagtgaga
     2881 ttgcccacat ctaacaatga ggcatgcgcc gtgacaggca gtggatgagt aagcggatgc
     2941 attctcactc catcgcatgg agaaaacggg tgattgataa agcaatcatc gttcttgggg
     3001 cgttaattgc gctgctggaa ctgatccgct ttctgcttca gcttctgaac tgatagcgga
     3061 aacgtaatta agggctaaga gcacactact cttagccctt taacatttaa cgcatggtca
     3121 cgaactcttc tgccgccgtt gggtgaatgg cgacggtatt gtcgaagtct tttttggttg
     3181 cgcccatctt cagcgccacc gcgaagccct gcaacatttc gtccatacca aagccaatgc
     3241 cgtgaatacc gacaatcttc tcttctggcc caacgcacac cagcttcatg cggcacggct
     3301 ggcggtgagt ggtgacggcg gtatacatcg cggtgaaaga ggatttatac actttcacct
     3361 gatcgtcgcc gtactgctcg cgcgcctgcg gttccgttaa accaacagta ccaatcggcg
     3421 gatggctgaa gaccacggtc ggaatgttgc tgtaatccag atgctcatcc ggcttgttat
     3481 taaacaggcg ttcagagaga cggcgacccg ctgcaactgc caccggtgtc agttccactg
     3541 cacccgtgtt atcgcccacc gcgtaaatac cttcaatatt ggtgttttga tatttatcga
     3601 cgacgatata gcctttttcg ttggttttaa cgccagcggc ttcgaggttg atgttgtcat
     3661 tggcaggctc gcgaccaatc gcccaaatca ggcaatccac cgtttcactg cgaccatctt
     3721 ccagctccag cgtcaggcta ccatcggcat ttttcactac cgctttcggg atggcgttgg
     3781 tatgcaactg tgggccttcg gcgttcatca cttcgaccag cgtttcagag atcatcgggt
     3841 cgaagctgcg cagcggcgca tgtttacgca caaacagatg cgttttcgcg ccgaggccgt
     3901 taatcacgcc agccagctca acggcgatgt aacccgcgcc aacaaccgcc acgcgctctg
     3961 gcaaagcagg aagggcgaag aagccatcgg aatcaatacc gtattccacg cctggaatat
     4021 ccgggtggct cggacgaccg ccggtggcga tcagaatatg atcggccgtg atggtttcgc
     4081 cgttaacttc cagcgttttg gcatcaacga agcgggcaaa gcctttgatt acatcaacgt
     4141 tatttttacc gagcacgttt tcataggaag tatgaatacg gtcgatatag gcggtacggc
     4201 tggcgatcaa cgtttcccag ttgaacttgt tgatggtggt atcgaaacca taatcagggc
     4261 cgtacatatg gatcgcttca cggatttgcg ccgcgtgcca catcactttt ttcggcacgc
     4321 agccgacgtt cacgcaggtg ccgcccagct ctttggcttc aatcagtgcg catttctggc
     4381 cgtacatagc cgcgcggttg atggaggcga taccgccgct gccgccgccg atggcgatgt
     4441 aatcatagtg tttagtcatg acatattgtc cttatcgttg attaccgcga ttgtagcgcg
     4501 agacgtaata ggtgccagca atggctgcaa ttactccggc acgatccagc ttacggtggc
     4561 gtgcccggta cctgccggaa ccagtttgct gtgcagccac gacagcacgt tattcatctg
     4621 ctgttccagc ttccacggcg ggttaatcac aatcatgccg gaagcggtca tgccacggcg
     4681 atcgctgtct ggcagcaccg ccagttcaat ttgcagaatt ttgcgaatgc cggtcgcttc
     4741 caggtcgtgg atcatgcgct taatttgctg gcgcagcacc accggatacc acaatgcgta
     4801 agtgccagtg gcgaaacgtt tgtaaccttc tgctatcccg ctgaccaccg cctgatagtc
     4861 ggttttcatt tcatacggcg ggtcgataag gattaaacca cggcgagaaa ccggcggcag
     4921 tttggcctta agttgctgga aaccgtcggc tttttcgacg cgtgcacggc tgtctttctg
     4981 aaatccagaa cgcagcaacg ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc
     5041 ttgttcgcgc agtagctggc gagcaatcaa cggcgaaccg gggtagtaac gcaactggcc
     5101 gctacggttg aagtgtttta ccacattgat gtacgcctcc agttctgcgg gcaaatcgtc
     5161 ctgctgccag atacgggcga taccttcgag atattcgccg gtacgctcgg catgttcgct
     5221 acctaactga taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt
     5281 ctctttcagc gactcgatga tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt
     5341 gccagcgtga aagctgtggc gataactgag catgggtaaa ggtgttccgg taagtaaaaa
     5401 ttggcccgta ttcggggcgc acaaaagcct atcaggacag tataccgaaa agaggccgcc
     5461 gccgcgaaag cgtaacgttt ctcattgaaa ttcactacac ttaaccccat gctacacaca
     5521 ttatgtaaag cgcctgttga gcgcttcctt aacctcttta accaggactg cgctaatgac
     5581 gaatccgtta ctgactccct ttgaattgcc tccgttttct aaaattctcc cggaacatgt
     5641 cgttccagcc gtgactaagg cattgaacga ctgccgcgaa aatgtggagc gcgtagtagc
     5701 gcaaggggca ccgtacacct gggaaaatct ctgccagccg ttggcggaag tggacgatgt
     5761 tttggggcgt atcttctccc cggtcagcca cctgaactcg gtgaaaaata gcccggaact
     5821 gcgtgaagcg tacgaacaaa ccctgccgct gctgtcggaa tacagcacct gggtagggca
     5881 acatgaaggg ctgtataaag cgtaccgcga cctgcgcgat ggcgatcatt acgccacgct
     5941 gaacacggcg cagaagaaag cggttgataa cgccctgcgt gattttgaac tctctggcat
     6001 aggtctgccg aaagagaaac agcagcgtta cggcgaaatt gctacccgtc tttctgaact
     6061 gggcaaccag tacagcaaca acgtcctcga tgcgacgatg ggctggacca aactcgttac
     6121 cgacgaagcg gggctggcgg ggatgccaga aagcgcgctg gctgcggcaa aagcccaggc
     6181 cgaagcgaaa gagctggaag gctacctgct gacgctggat atcccaagct acctgccggt
     6241 aatgacctac tgcgacaacc aggctctgcg cgaagagatg tatcgcgctt acagcacccg
     6301 cgcctccgat caaggcccga atgccggtaa gtgggataac agcaaggtga tggaagagat
     6361 cctcgcgctg cgtcacgaac tggcgcaact gctgggcttt gaaaactacg cctttaaatc
     6421 ccttgccact aaaatggcag aaaacccgca gcaggtgctg gatttcttaa ccgatctggc
     6481 aaaacgcgcg cgtccgcaag gcgaaaaaga gctggagcaa ctgcgtgcct tcgctaaagc
     6541 cgaatttggt gtcgatgagt tgcagccgtg ggatatcgct tactacagcg aaaaacagaa
     6601 acagcacctc tacagcatca gcgacgagca gctgcgtccg tacttcccgg aaaacaaagc
     6661 ggttaacggc ctgtttgaag tggtgaaacg tatttacggc atcaccgcta aagagcgtaa
     6721 agatgttgat gtctggcatc cggatgtacg tttcttcgaa ctgtatgacg aaaataacga
     6781 actgcgcggc agcttctacc tcgatctgta tgcccgtgaa aacaagcgcg gcggggcgtg
     6841 gatggatggc tgcgtaggcc agatgcgtaa agctgatggt tctctgcaaa aaccggtcgc
     6901 gtatttgact tgtaacttca accgcccggt aaatggtaaa ccggcgctgt tcactcacga
     6961 cgaagtgatc accctgttcc acgagttcgg tcacggcctg caccatatgc tgacccgcat
     7021 cgaaaccgct ggtgtttccg gtatcagcgg tgtgccgtgg gatgcggtcg aactgccgag
     7081 tcagtttatg gaaaactggt gctgggagcc ggaggcgctg gcgtttatct ctggtcacta
     7141 tgaaaccggc gaaccgctgc cgaaagagtt gctggataaa atgctggcgg cgaagaacta
     7201 ccaggcggcg ctgtttattc tgcgtcagct ggagttcggc ctgtttgatt tccgccttca
     7261 tgccgagttc cgcccggatc agggggcaaa aatcctcgaa actctggcag aaatcaagaa
     7321 actggttgcc gtggtgccat ctccgtcctg gggccgtttc ccgcacgctt tcagccatat
     7381 tttcgccggt ggttatgcgg caggttacta cagctacctg tgggctgacg tactggcggc
     7441 agatgctttc tcgcgctttg aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt
     7501 cctcgacaac attctgagcc gtggcggttc agaagagccg atggatctgt tcaaacgctt
     7561 ccgtggtcgt gaaccgcagc tggatgcgat gctggagcat tacggcatta agggctgatc
     7621 attcagtgaa aatctgctta attgatgaaa caggcgccgg agacggcgcc ttatctgttc
     7681 tggcggcccg ctgggggctg gagcacgatg aagacaacct gatggcgctg gtgttaacgc
     7741 cggaacatct ggaattgcgc aagcgtgatg agccaaaact tggcggcatc tttgttgatt
     7801 ttgttggcgg agcgatggcg caccgacgca aattcggcgg tggtcgcggt gaggcggtgg
     7861 cgaaagcggt gggcattaaa ggcgattatt tgccggatgt ggtggacgcc accgcagggc
     7921 tggggcgcga tgcctttgta ctggcttcag tcggctgccg cgtgcggatg ctggagcgta
     7981 atccagtggt tgccgcgcta ctcgacgacg gcctggcgcg tggttatgcg gatgcggaaa
     8041 tcggcggctg gttgcaggag cggttgcagt taattcacgc ctccagcctg acggcgctaa
     8101 ccgatattac cccacgcccg caggtggtct atctcgatcc gatgttcccg cataagcaga
     8161 aaagcgcgct ggtgaaaaaa gagatgcgtg tctttcagtc gctggtgggg ccggatcttg
     8221 atgccgatgg attactggag cctgcacgct tgctggcgac caaacgcgtg gtggtaaagc
     8281 gcccggacta cgcgccaccg ctggcgaatg tcgccacgcc aaacgcggtt gtgactaaag
     8341 ggcatcgctt tgatatctat gcaggtacgc cagtgtaaaa gtaaagcagg gtagcgataa
     8401 cgctaccctg ctttctgcaa gaataattaa tggctttccg gcgtcgcaat catgcgtttc
     8461 agccacggca ccatcaacag catcactact gcaacgccca gcgtgaccag accaatctta
     8521 ccaaacacgt tggtatagac gggcaacgtc tcaagcggat cggtaatgtt gtccggcacc
     8581 gcggtaaatg ttgccacata gccgcccagc aagaacgcgg cagcctgcgt caggaaccac
     8641 atcccgagaa taaagcccat caaatgctgc ggcaccaggg cagcaatcat cgccaggcca
     8701 agggcgctaa taaacaattc acccaggctc tagaataaat acaccagcac gataaaccat
     8761 ggcgatgtca gcccctggtc atccgcaaac cacattcccg cagcagcagc cgtcaaaaag
     8821 cccagtgagc acataaacat gctgagagta aatttcatcg gcatcgagag gtctttgcct
     8881 ttgttaccct gatgcgtgta aatgcctgcc agtattgggc tggcgagtac cacccagaac
     8941 gggttaagcg cctggaagct gaccgggttg atggaaaaac cgagaatttc atgatgcacg
     9001 ttgttgatgg caaagaagtt cagcgatgtt ggcatctggg cgtagagaat gtaaaacacc
     9061 accgcttcga gcatcaggac aaaggcgaca aacattttat tgcgcccggt tttatccagc
     9121 ttgaatgcct gacgaaagaa gatgatagtg acgacgatgg agagaacaat cagcaccaga
     9181 ttggcgactt ctacgttgtg catcagccat gcacatacga agatcatcac cacgctgcca
     9241 agtaatacgt acaacagttt gctgaagctc atcggcttga agtcgggttc agaaccaatg
     9301 tctttcacca ttccacgaca ggcgatgtaa accagtaatg cgataattaa ccccgccccg
     9361 cacaggttgt aggtgactga ataaccgaat ctatcagcga tcacaggggc cagcgataac
     9421 gctatcaacg agccgatatt gatcgacata tagaacaggg tgaatgcgcc atcaagccgc
     9481 ggatctttcg gcggatagca cttcgaaagc aagctggctg ggttagcttt aaacaggccg
     9541 ttaccgacag cgatagtccc cagggcgatg aaaatcaggt caggcttaag tagcgacatg
     9601 ccggtcatga agtagccaat cgccagcaca agtgctccaa gaacaatggt gcgtttggtc
     9661 cccaacaggt ggtcgccgac atagccgcca atggaaatga ggccatagac cagtgcagca
     9721 aaagcaccaa aagtgacaaa agcctgctct tgcgagaatc caagctgttt aacgaagaaa
     9781 accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc gacaaaaaag
     9841 atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt attaatattt
     9901 attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag
     9961 tttacatctg tcagagattc ttaatattga tgcggtaaat agattaatac actattacaa
    10021 cagaaaataa ccagctgatc acactaatga tggaaaatat gatggtgtta aattttgtat
    10081 tggcaggaga ttttgattgc gtacgcagat cgtaggccag ataaggggtt tacgctgcat
    10141 caggtaacaa ggcgcgccaa aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt
    10201 cgtggaggga tgattattct tcttcgtcgc gtagcggaac aatcagcata tcaacgtgaa
    10261 cggtgttgat cagctgacgt gcggaagaca tcagtttgct ccagaagtcc tggtggtgac
    10321 cacaaaccac cagatccata tcgtatttct tgattgcatc gaccagaacc tggcctaggt
    10381 cgccgctgcc gctcagggtt tcagtgattg gatagcctgc attagtggaa agctcggtca
    10441 gcgcatgatg tgtctcttca gagatgcgtt tctgcatatc acccagattc acatcaataa
    10501 gcccggtgta taggtcagag tagtttacat ctacgtggat cagagaaact ttcgcattgt
    10561 aggggcgagc catagagact gctttctcta ccagaacttt gctttccggg gagagatcga
    10621 ctgcgatgag aatgtgttta taagccatag tgttactcct tccataaagt tgtcgatgac
    10681 tggccagcta gcgtttcttg tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg
    10741 ggctaaccca gccgtaaaga ttcagtgtta agaccatcct taccttatag cgacccggat
    10801 gatccgtcaa tccgccttgc ttacccgtta gataaacaaa ttttcgacat gaatattata
    10861 ttgatagtgg ttaaccttct ggaaaaaaaa caacctgatc tcctacacta tctatagagc
    10921 cgctcggatg ttggtcacca caaaagcggt ttctccagga cctggtgtta actgattggc
    10981 agcgtatcgg gagcggtagc cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg
    11041 tatgataagc accgtcgcat tattttgggc tttatgtgtc gtttgcattg ttaacatggc
    11101 gcgctatttc tcatcactac gcgcgttgtt agtggtactg cgtaactgcg atccattgct
    11161 ctatcaatat gttgatggag ggggcttttt tacctcacat ggccaaccca acaaacaggt
    11221 gcgtctcgtt tggtatatct atgcccaacg ttatcgcgat catcacgatg atgagtttat
    11281 tcgccgctgt gagcgggtgc gtcggcagtt tattctgact agcgcattgt gtggtctggt
    11341 ggtggtcagc ctgattgcat tgatgatttg gcattaagca cttcagcaat aataacgtat
    11401 aaaaaaagcg ggccagtaaa ctgacccgct cgttttggtg cgtacgatta caggaactgc
    11461 aaggagaacc agtacagccc gccggaaagt agtaccgcag ccggaagggt aaacacccag
    11521 gccatcagaa tactggttac ggttttacgc tgtaagccgc cgccatctac caccatcgtc
    11581 cccgcgacag aagaggagag tacgtgagtg gtggaaaccg gcatcccggt ataactcgcc
    11641 aggccgattg acactgccgc cgtcatctgg gcagacatcc cctgagcgta ggtcatgcct
    11701 ttcttaccga ttttctcacc gatagtcgtt gccacacgac gccagccaat catcgtaccg
    11761 atacctaacg ccagcgcgac cgccatgatg atccacaccg gcgcatactc gatggtgcta
    11821 agcatgtcgg acttcagttt cttcaacagg cgctgatcgt cagcactcac accaggcatc
    11881 ttcaccactt tgtcgatagt gtcagaaacg cacagcataa tgcggcgcat ctggctacgt
    11941 tgatcaagcg acagcttgtc gtagctttcc acatcggtgg tcaacatacc tttcaggcgg
    12001 ttgagcgcgt taatggtatt cgacggatgg cagtggaact ccgcaggttg cgttgcgcca
    12061 gcttccggag ccggtactaa ctgatcagca ccggtagcct gtttgagcag cgcaggatgc
    12121 tgctcaaagt aagcttcgac gttgttgatg gcatcacggg tacgggtgat ttcgtagcca
    12181 gtggcattca tgttcaccac gaagcctgct ggcgcgacgc caatcaatac caacataacc
    12241 agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa acgccacgcc gatagcggaa
    12301 aggatcagcg cgatacgcgt ccagaacggc ggctttttct tgccgtcttt cttttcacgc
    12361 tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc cgctccagta gcgacgcagc
    12421 aagaaaatca gaccgccagc aaacaccagg ccgacaatag gggaaacgat cagagaaccg
    12481 aaaatactta atactttcgg gatattgagt gcatccacca ctgacgtccc ggtcatcaac
    12541 gcattggtta aaccaatccc gatgatcgcg ccaatcagcg tatgagagct ggatgcaggt
    12601 aaaccaaagt accaggtacc caggttccag ataatcgccg ccagcaacat agagaacacc
    12661 atggcaaggc catgagacga tcccatatta agcagcagat ccgtcggcag catatgcaca
    12721 atggcatagg caacactcag accacccagc aaaacaccca aaaagttgaa caccgccgcc
    12781 ataaccacgg cgagctgaga acgcatcgcg cgggtataga taacggttgc cacggcgttg
    12841 gctgtgtcat ggaaaccatt gatggcttcg tagaacagca caaaagccag tgcaagcaat
    12901 aataacagcc cggtatgcaa atccaggcca gcaaacaaat gtagcatagg acgttacgcc
    12961 attttgagga catgaacgcg gcgcattatc agtgactttc gcggcgcggg caaagtgaaa
    13021 tatagacttt ttttgatttg cctcctgtat ggatttcact caaaaaataa ttatcttata
    13081 taattcaggc aaatacttcc ttttagtaat attgatgctg gtgcgaccac tgaggaatct
    13141 ttacaattca cgcccgtttt ttctaagagg agcgcaacgt ggaaaggttt gatgccatta
    13201 ttataggcgc tggtgcggcg ggtatgttct gttctgcgct ggcaggtcag gtaggacgcc
    13261 gggttctgct gatcgataat ggtaaaatac tagggcgcaa aatccttatg tctggcggtg
    13321 ggcgctgcaa ctttaccaac ctttatgtcg aaccaggcgc ttatctgagc cagaatccgc
    13381 atttttgtaa gtctgcactc gcacgtttta cccagtggga tttcattgat ctggtcaata
    13441 aacacggtat cgcctggcac gagaaaacgt tagggcaact cttctgcgat gactccgcgc
    13501 agcagattgt cgacatgctg gtggatgagt gcgagaaggg caatgtaacc ttcagattgc
    13561 gtagcgaagt gctgagtgtg gcgaaggatg aaacaggctt cacgcttgat ctgaacggca
    13621 tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg gctgtcaatg ccggggctgg
    13681 gcgcatcgcc gtttggttat aagattgccg aacaatttgg cctcaacgtg ctgccgaccc
    13741 gcgcgggtct ggtgccattc actctgcata aaccgttgct cgaagagtta caggtgctgg
    13801 cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg caccgttttc cgtgagaact
    13861 tactcttcac ccaccgcggc ttatctggac cggcggtgtt gcagatttca agctactggc
    13921 aaccgggtga atttgtcagt atcaatctgc taccggatgt ggacctcgaa accttcctga
    13981 atgagcagcg taacgcacat ccgaatcaaa gcctgaaaaa cacactggcg gttcatctac
    14041 cgaagcggtt ggttgaacgc ttacagcaac tcgggcaaat cccggatgtt tcgctaaaac
    14101 agctcaacgt gtgtgaccaa caggcactga ttagcacatt gaccgactgg cgcgtacaac
    14161 ccaacggcac tgaaggctat cgcactgccg aagtgacgct cggcggcgtg gacaccaacg
    14221 aactttcttc acggacgatg gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag
    14281 tgatggacgt taccggctgg ctggggggct ataacttcca gtgggcgtgg tcgagtgcat
    14341 gggcttgtgc gcaggatttg gcgtcaattg tgaaataacc atgacgtttt cccgtcagcc
    14401 gtttgtatat gtcgttatta agtcattttg gtacggttct gcatccgaca ttctggtgtt
    14461 aactcgctgt tttgttagcg aattaacgct attgccgttg tggttatttt atagtgtatt
    14521 tattctcaga gaatatatat tactgaaata aaaaacacaa agaataacca aaggaaatta
    14581 tttgaaacgt aaaaggtata tatttttaat ctgtaaaatt taaaatcacc tcctctggat
    14641 tgaatcaact tgctctattt aagacgattg tttttacatt ttcaccacct gtatcaggtg
    14701 gtgttttttt attggcggca atgcctttat cttattttta ttacgttgta aatatggcta
    14761 ttcctaaatg aaatttgatt tgctcagttt atttgtgatg tcaggggagt gcttgccatg
    14821 agagtcgtga tattattatt catcattttt aggtaatgct gccaacttac tgatttagtg
    14881 tatgatggtg attttaaggt gct
//
LOCUS       DBKELA010000098        14478 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.098, whole genome shotgun sequence.
ACCESSION   DBKELA010000098 DBKELA010000000
VERSION     DBKELA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.098"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..115
                     /locus_tag="ACUQR0_003140"
     CDS             <1..115
                     /locus_tag="ACUQR0_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HHV9110079.1"
                     /translation="TISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(112..378)
                     /locus_tag="ACUQR0_003141"
     CDS             complement(112..378)
                     /locus_tag="ACUQR0_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hemolysin"
                     /protein_id="HHV9110080.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            652..4176
                     /gene="nifJ"
                     /locus_tag="ACUQR0_003142"
     CDS             652..4176
                     /gene="nifJ"
                     /locus_tag="ACUQR0_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HHV9110081.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4543..5672
                     /gene="ompN"
                     /locus_tag="ACUQR0_003143"
                     /pseudo
     CDS             4543..5672
                     /gene="ompN"
                     /locus_tag="ACUQR0_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5813..6247
                     /gene="uspF"
                     /locus_tag="ACUQR0_003144"
     CDS             5813..6247
                     /gene="uspF"
                     /locus_tag="ACUQR0_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HHV9110082.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6423..7358
                     /gene="ttcA"
                     /locus_tag="ACUQR0_003145"
     CDS             6423..7358
                     /gene="ttcA"
                     /locus_tag="ACUQR0_003145"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HHV9110083.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7487..8854)
                     /gene="dbpA"
                     /locus_tag="ACUQR0_003146"
     CDS             complement(7487..8854)
                     /gene="dbpA"
                     /locus_tag="ACUQR0_003146"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HHV9110084.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8884..9057)
                     /gene="ynaL"
                     /locus_tag="ACUQR0_003147"
     CDS             complement(8884..9057)
                     /gene="ynaL"
                     /locus_tag="ACUQR0_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HHV9110085.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9332..10315)
                     /gene="zntB"
                     /locus_tag="ACUQR0_003148"
     CDS             complement(9332..10315)
                     /gene="zntB"
                     /locus_tag="ACUQR0_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HHV9110086.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10329..10376)
                     /gene="ydaN"
                     /locus_tag="ACUQR0_003149"
     CDS             complement(10329..10376)
                     /gene="ydaN"
                     /locus_tag="ACUQR0_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HHV9110087.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10570..11802
                     /gene="dgcM"
                     /locus_tag="ACUQR0_003150"
                     /pseudo
     CDS             10570..11802
                     /gene="dgcM"
                     /locus_tag="ACUQR0_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11823..12386)
                     /gene="smrA"
                     /locus_tag="ACUQR0_003151"
     CDS             complement(11823..12386)
                     /gene="smrA"
                     /locus_tag="ACUQR0_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HHV9110088.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12640..14094)
                     /locus_tag="ACUQR0_003152"
     CDS             complement(12640..14094)
                     /locus_tag="ACUQR0_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HHV9110089.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14091..>14478)
                     /locus_tag="ACUQR0_003153"
     CDS             complement(14091..>14478)
                     /locus_tag="ACUQR0_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HHV9110090.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3860 a         3652 c         3420 g         3546 t
ORIGIN      
        1 caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga accagttaac
       61 gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga attaatagct
      121 gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg gtaatgcaca
      181 catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc cgatcattgc
      241 acaatttgct tctccactac tggacatcga cgcttttaaa cttggcgcta cgtgcgccgc
      301 agtcgcctgc tgaacaggct cactactaca cgccgacaac aataaagcgg cacaccctac
      361 ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac accgccataa
      421 ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc cgcattttta
      481 cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac tctgatgtgg
      541 gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg attactactg
      601 ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca tatgattact
      661 attgacggta atggcgcggt tgcttcggtg acgtttcgca tcagtgaagt tatcgccatc
      721 taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc cggaaacggc
      781 ttgaagaacg tttggggaga cacaccacgc gtggttgaaa tgcagtcgga agcgggtgct
      841 atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt tacgtcatcg
      901 cagggtttgc tgctgatgat cccgacgctg tacaaactgg caggcgaact gacgccattt
      961 gtcctgcatg tagcggcacg taccgttgcc acacatgcac tttctatttt tggcgatcat
     1021 tccgacgtta tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc taacgtccag
     1081 gaagcgcaag actttgctct catttcgcac atcgcaacgc tgaagagccg cgtgccattt
     1141 attcatttct ttgatggttt tcgcacgtcc cacgaaatca ataaaattgt cccgctggcc
     1201 gatgacacta ttcttgatct catgccgcag gccgaaattg atgctcatcg cgcccgggcg
     1261 ctcaacccgg aacatccggt gatccgcggt acgtccgcca atcctgacac ttatttccag
     1321 tctcgcgaag ccaccaatcc ctggtacaac gcggtctatg accatgttga acaggcgatg
     1381 aatgatttcg ctgccgcgac aggtcgtcag tatcagccgt ttgagtatta cgggcatccg
     1441 caagcggaac gggtgattat cctgatgggc tctgccattg gcacctgtga agaagtggtt
     1501 gatgaattgc taacccgtgg cgaaaaagtt ggcgtgctga aagttcgtct gtaccgtccc
     1561 ttctccgcca aacacttgct gcaagctctg ccgggatccg tacgcagcgt ggcggtactg
     1621 gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt gatgaccgca
     1681 ctggcagaag cctttaataa tggtgagcgc gaaaccctgc cccgtgtcat tggtgggcgc
     1741 tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt tgccgagctc
     1801 aacgcggcta aacctaaagc acgctttacg gttggtattt acgatgatgt gaccaatctg
     1861 tcactgccgt tgccggaaaa cactctgcca aactcggcga aacttgaagc cttgttttat
     1921 ggccttggta gtgatggcag cgtttccgca accaaaaaca atatcaagat tatcggtaat
     1981 tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc gggcggcctg
     2041 acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct catttcccag
     2101 gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat ggctgagcgt
     2161 ttaaaaccta gcggcatttt cctgcttaat acgccgtaca gcgcagatga agtgtggtcg
     2221 cgcttgccgc aggaagttca ggccgtgtta aaccagaaaa aagcgcgctt ctatgtgatt
     2281 aacgcggcga aaatcgcccg cgaatgtggc ctggcggccc gtattaatac cgtcatgcag
     2341 atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc agaattgcag
     2401 ggtgcgattg ccaaaagtta cagtagcaaa ggtcaggatc tggtggagcg caactggcag
     2461 gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt aaatccgcac
     2521 agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa aaccgtaacc
     2581 gcggcgatgc tcgccgggct tggtgatgcc ctccccgttt cggcgctgcc gccagacggc
     2641 acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga gatccccatc
     2701 tggaaagagg aactctgtac ccaatgtaac cactgcgtcg ccgcttgccc acactcagct
     2761 attcgcgcaa aagtggtacc gcctgaagcg atggaaaacg cccctgccag cctgcattcg
     2821 ctagatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt ggcaccggaa
     2881 gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg tcagaatcca
     2941 gagattaaag ccatcaatat gatgtctcgc caggaacatg tcgaagaaga gaaaatcaat
     3001 tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg tattgatatt
     3061 cgtacatcgc agctgattac accgctgttt gaatattcag gtgcttgccc cggttgtggc
     3121 gagacgccgt atattaaatt gctgactcag ctctatggtg accggatgtt gatcgctaac
     3181 gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta taccaccgat
     3241 gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc cgaatttggc
     3301 cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa
     3361 tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc tacgccagag
     3421 gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc cgaagcacat
     3481 gaactgctgc gtgacgcaga tgcactggtg gaaaaatcaa tctggctgat tggtggcgac
     3541 ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt gacggaaaac
     3601 gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa
     3661 gcgacaccgc tgggtgcagt aactaagttc ggcgagcacg gcaaacgtaa agcgcgtaaa
     3721 gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat ttctctcggc
     3781 gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc gggaccatcg
     3841 ctgatcattg cttatagccc gtgtgaagag catggttacg atctggctct cagccacgac
     3901 cagatgcgcc aactcactgc taccggcttc tggccgctat atcgctttga tccgcgtcgt
     3961 gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga agcactggaa
     4021 gaaacgttac ttcacgagca gcgtttccgt cgtctgaatt cgcagcagcc agaagtggca
     4081 gaacagttat ggaaagatgc tgcagctaat ttgcaaaaac gctatgactt cctagcacaa
     4141 atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag gattatccaa
     4201 ttctaaaaaa aaagcccgga cgactgttcg ggcttgtctt tttatatgtt ggaaaatcag
     4261 tggcaatgca atggcccaac agaaaataaa atctgtgaca ataaaggcat ataacccgcg
     4321 cagaataacg tataggaaat taaaagtata atttttattt tgtatgtatt atcttcgata
     4381 cattcaccag acttattatc atttcagagg aattatttga ttaaggtttt acttaaggcg
     4441 taacaaatga tatttatcgg ctaactgaac ttctccttta ggatgttttc actccccgaa
     4501 atggggataa aaggcaaata aaataactaa aggatttatt caatgaaaag aaaagtactg
     4561 gcacttttaa ttcctgccct gctcgccgca ggtgctgcac atgcagccga agtttataat
     4621 aaagacggca acaaattaga tctgtatggc aaagttgatg gcctgcatta tttttctgat
     4681 aattcagcga aagatggcga ccagagctat gcgcgtctgg gttttaaagg cgaaacccaa
     4741 attaacgatc aacttactgg ctacggtcaa tgggaataca atattcaggc aaacaacact
     4801 gaatcttcaa aaaaccagtc atggacccgt ctggcatttg ccggactgaa atttgcagat
     4861 tacggttctt tcgattacgg acgtaattat ggcgtaatgt acgacatcga aggctggacc
     4921 gatatgctgc ctgaatttgg cggtgactct tataccaatg cagacaactt tatgactggt
     4981 cgagccaatg gcgtcgcgac ttatcgtaat actgatttct tcggtctggt aaatggtctg
     5041 aacttcgcgg tgcagtatca aggtaacaac gaaggagcca gtaatggtca ggaaggcacc
     5101 aataacgaac gcgatgttcg tcatgaaaac ggtgacggct ggggtctttc cacaacatat
     5161 gatttaggca tgggctttag cgctggtgcg gcatacacct cttctgaccg caccaatgac
     5221 caggttaacc atactgcggc gggtggtgat aaagcagacg cgtggactgc tgggctaaaa
     5281 tacgatgcta acaatattta cctagcaacc atgtattcag aaacgcgtaa tatgaccccg
     5341 tttggcgaca gcgattatgc agttgcgaac aaaacccaga actttgaagt tactagtacc
     5401 agtttgattt tggtctgcgt ccggcagtct ctttcctgat gtctaaaggc cgtgaccttc
     5461 acgctgctgg tggtgcagac aacccggcag gtgttgatga taaagatctg gttaaatacg
     5521 ccgatgttgg cgcgacttac tatttcaata aaaacatgtc cacctatgtt gactataaaa
     5581 tcaacctgtt ggatgaagat gacagtttct acgctgccaa cggcatctct accgatgata
     5641 ttgtcgcttt aggtctggtt tatcagttct aaatcctcct gcccgctgtt atggcgggct
     5701 ttttctgctt attcttcctc ctttgatcta aattaaaaat gtgaactccg tcattacaca
     5761 aaaagtgtca tctggcgtta cactttatgc ggatactaaa acaggaggtt ttatgaacag
     5821 aacgattctt gtccctatcg atatttcaga ttcagaatta actcaacgcg tgattagcca
     5881 cgttgaggca gaggcaaaga ttgatgatgc agaggttcat ttcctgacgg taataccttc
     5941 actgccctac tatgcctctc tgggtttagc gtattccgca gaattaccgg caatggatga
     6001 cctgaaagcg gaagccaaat cgcaactgga agagatcatt aaaaaattta aactgccaac
     6061 cgacagagtg catgtccatg ttgaggaagg ctcgcccaaa gaccgcattc tggaattggc
     6121 gaagaagatc cccgctcata tgatcatcat tgcttcccat cgaccggata tcaccactta
     6181 tctgctcggt tccaacgccg cagctgtagt gcgtcacgca gagtgctccg tgctggttgt
     6241 gcgctgacac taacgcccgc acattgctgc gggctttttg attcatttcg caaatgtgct
     6301 gacattttcc cctctaatcc gtaccataca cgccacagtt tttatatcag atttcttatg
     6361 ctgagcgttc cggcatgacg catactcttc tgatgccata taacgaattg agtcgctttt
     6421 aaatgtcgca aaatcaagaa attagtaaga aagaacaata caacctgaac aaattacaaa
     6481 aacgtctgcg tcgtaacgtg ggcgaagcca ttgctgactt caatatgatt gaagaaggcg
     6541 atcgcatcat ggtttgcctc tccgggggta aagacagcta taccatgctg gagattctgc
     6601 gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt ggctgttaac ctcgatcaaa
     6661 agcaaccggg cttcccggaa cacgttctgc ccgagtatct tgaaaagctg ggcgttgagt
     6721 acaagattgt tgaagagaac acttacggta tcgtgaaaga gaagattcca gagggcaaaa
     6781 ccacttgctc actgtgttct cggctacgtc gcggtatcct ttatcgcacc gcaacggaac
     6841 tggggacgac gaagatcgcg ttgggtcacc atcgtgacga tatcctgcaa acgttgttct
     6901 taaatatgtt ctacggcggc aagatgaaag gtatgcctcc gaaactgatg agcgatgatg
     6961 gcaaacatat cgttattcgt ccactggcct actgccgcga gaaagatatt cagcgatttg
     7021 ccgatgcaaa agcgttcccg attattccgt gcaacctgtg cggttcacag cctaacctgc
     7081 aacgtcaggt gattgctgac atgttgcgtg actgggataa acgttatcca gggcgtatcg
     7141 agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca tctgtgcgat accaacctgt
     7201 tcgatttcaa aggcattacc cacggttctg aagtggttaa cgggggtgat ctggcgtttg
     7261 atcgcgaaga gatcccacta caaccggcgg gctggcagcc agaagaagat gaaaatcagt
     7321 tggatgagtt acggctgaat gtggttgaag tgaaataacc aagatagcgc ccgatgcgca
     7381 agcgtatcgg gctactctta tggaggccgg ataagacgcg gccagcgtcg catccggcaa
     7441 tcccgaataa gatgtttact cttgcacccg gcaattcaac atttcattat tttaataacc
     7501 gcacccggca cgtttttcct ttaatcttcc cgccctgtaa ctgtttccat gctttatgag
     7561 caacagcctg acggaccgcg acatagacat gcgccggatg cacggcgatt ttgccaatat
     7621 ctgcgccatc aagcccgata tctcctgtca gtgcacctaa tacatcaccc gggcgcattt
     7681 tggctttttt cccgccatcg atacacaacg ttgccatttc tgcttccagc gtcacaatgg
     7741 aactattagc tggcggcgtt tgccagttaa gttttatctg caacatgtca gaaatgatat
     7801 tggcccgctg tgcttcttcc ggagcacaga aactgatcgc cagaccgcta tttcctgcac
     7861 gagctgtacg accgatgcga tgtacatgaa cttcagggtc ccacgccagc tcaaagttca
     7921 ccaccagctc aagcgattta atatccagac cacgcgcagc aacatcagtc gcaaccagta
     7981 cacgggcgct accgttagca aaacgtacca gggtctgatc gcgttgctcc agatcgccgt
     8041 gtaacgacaa tgcactttgc tctacttcat tcagcgcgtc gcagacagcc tggcaatctt
     8101 ttttggtatt gcaaaacacc acacaagagg atggctgatg caagcttaat aaccgttgca
     8161 acagaggaat tttgccttta ctggatgtct cataaaattg ttgttcaatg ggtggcaaag
     8221 catctgttga gtcaatttca atcgccaaag gatcgcgttg cactcgtccg ctgattgcgg
     8281 cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg tcgagatgca ggcgcaaaac
     8341 ggatgacatc atcaatggca tcgctaaatc ccatatccag catgcggtcg gcctcatcca
     8401 tcaccaaagt attcagcgca tccagtgata ccgtgccttt ttgcaagtga tccagcaaac
     8461 gacccggcgt tgccacgata atatgcggcg catgttgcag cgaatcacgc tgcataccga
     8521 acggttgacc accgcacaac gtcaaaattt tggtatttgg cagaaaacgc gccagccgac
     8581 gcaattcacc tgccacctga tccgccagtt cacgcgtagg acacagcact aaagcctggg
     8641 tttgaaatag cgacgcatca atttgctgta acaagccgag gccaaaagcc gccgttttgc
     8701 cgctgccggt tttcgcctgc acgcgaacat cttttccggc aaggatcgcc ggaagcgcgg
     8761 cggcctgcac cggcgtcatg gttaaataac ccaactcatt aaggttcgtg agttgggcgg
     8821 gaggcaaaac attcagggta gaaaaagcgg tcacaatcta ttctcgtggt catcgacgca
     8881 aagttagcag gcgcgtatcc tcgcagatct acgctcacga tgcgacaatt taatcggttc
     8941 ttcatcgggt ggtgggtcag gcatgggttg cgggcgaggg atcggatcgg gcactggaac
     9001 aggatcgcca ggaatcggtt cagggacagg aatttgcaaa taaataagtg tcgtcatatt
     9061 tccctccggt cattgggtgg actcttaaag ggtagacgct gataaataac aggcaaaaaa
     9121 aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt gctatgcagt aattcaaaaa
     9181 aggaagtaag acaatatgga gcgcaacgcc catcgcttga cgttgcattc acctgcaagg
     9241 gagatattgc cctgaatggg tagagagttt attgacttcg ctcaaacttt gcggcgtttt
     9301 tgtatacaga cagccggaaa aattgctttt gttacaacca tttactacgg tgcaaccata
     9361 aagcaacacc accaataaga acaactaaca gaatacaaaa aattgaaaat ccgaattgcc
     9421 acccgccgcc agggatccca ccaaggttga cgccaaataa cccggtcaga aaggtactgg
     9481 gtaaaaagac cattgccatc aacgacattg tataggtacg acgagctaaa ttttcctgca
     9541 tcacctgagc gatttcatcc gccatcacgc cagtccgtgc tatacaggcg tcgatttcgt
     9601 caaggccgcg cccaaggcga tcggcaatat cctgcatccg acggcgttgg tcatcgctca
     9661 tccacggcaa acgttcactg gcaagacgag cataaacatc acgttgcggt gccatatagc
     9721 gacgcatcac aattaattgt ttgcgcagca gagccaggaa tccacgcggt ggaatttgct
     9781 gatcaaggag attatcttca aggtcgataa ttttatcgtg cagctgctcg ataaattcac
     9841 tggaatgatc ggtcaacgca tcgcacacat ccaccagcca tcccccgcaa tcggtcggac
     9901 ccgtgccctc ttccagatcg ctcaccacat cgtccagcgc cagcactttg cgttgtcggg
     9961 tcgaaacaat taaccgcccg tccatatata cacgcatggc gaccagttga tcggggcgtt
    10021 catcggtgct gccgtttata cagcgcaatg taatcagcgt gccttcaccg aaacggctga
    10081 ctcggggccg tgtgctctcg cccgccagcg catcacgtac gttattggga agcagcggtg
    10141 ttgtcgccag ccattgggcg ctatcatggt gtacataatt aaggtggagc cagcagggat
    10201 gcgcttcatc aatcacatct gtattttcca gcggtttaac gccgcctcta ccatccagca
    10261 tccaggcaaa tactgcatcc gggacattaa cgtccgatcc cttaatcgct tccacagtgc
    10321 ctccatcatc aacgcattat tttgtagtct agccttctgg ccctgttacg caacatctca
    10381 tcaccccatt accctgaaat gattaataaa attctgtcta aattgaatac aaaaagcaaa
    10441 atgcttttcc gtatacaaac cgtgtgaagt gttaaatagc gtctatcatt atcagaatta
    10501 tctgatcata tgacgtggct tttttgcgat cggatagcaa caaaaattga taaaaataac
    10561 gggatctcaa tgattacgca caacttcaat accctggact tactcaccag tcctgtctgg
    10621 attgtttcgc cctttgagga acagttaatt tatgccaata gcgcggcgcg actgttgatg
    10681 caagacctca cgtttagtca gctacgaacc ggaccctatt ccgtctcctc acaaaaagaa
    10741 ctgccgaaat acctctccga tctgcaaaac caacacgata ttatcgaaat cctcactgtt
    10801 cagcgtaaag aagaggaaac agcattaagc tgtcggcttg ttttgcgaga gctgacagaa
    10861 acagaaccgg tgattatttt cgaaggtatc gaagcgccgg caacgctggg tttaaaagcc
    10921 agtcgctcgg caaattatca gcgcaaaaaa caaggttttt atgcgcgctt ttttctgact
    10981 aactctgcac caatgttgtt gattgacccg tcacgagatg gacaaatcgt cgatgctaac
    11041 ctcgccgcgc tcaatttcta tggttataac catgaaacga tgtgccagaa acatacctag
    11101 gaaataaata tgcttgggcg tcgcgtcatg cctatcatgc atgaaatttc gcatttaccc
    11161 ggcggtcata accctttgaa ttttgttcat aaactggcgg atggatctac tcgtcatgtg
    11221 cagacctatg ccggaccgat tgaaatttat ggcgacaagc tcatgttatg tattgtgcat
    11281 gatattactg agcaaaaacg gctggaggag cagctggaac atgctgctca ccatgacgcg
    11341 atgaccggat tactgaatcg gcgacagttt tatcacatta cggaaccagg ccaaatgcag
    11401 catctcgcca tcgctcagga ttacagcttg ttgctcatcg acaccgatcg ttttaaacac
    11461 attaacgatc tctatgggca ttctaaaggt gatgaggtgt tatgcgccct cgcccgcccc
    11521 ctcgaaagtt gcgctcgcaa aggcgatttg gtgtttcgtt ggggaggcga agagtttgtc
    11581 ttattgctac caagaacccc actggatacc gcgctttcgc tggctgaaac tatccgcgta
    11641 agcgtggcaa aagtgagtat ttcgggctta ccacgcttta ccgtcagcat tggtgtggcg
    11701 catcacgaag gaaatgaaag catcgatgaa ctgtttaaac gcgttgatga tgctttgtat
    11761 cgggcgaaaa atgatggacg caaccgcgtg ctggcggcat aagccgcgga tgcgtctcga
    11821 gatcaacgac tgcgcttagc gtggcgctcc cagttttctt gcttcgcctg cgccgtttta
    11881 cgtagtgcga cgtaacacgc cccgctgccg ccatgatgcg gtagcgcggt gcaatatgcc
    11941 tgaacatcat caaattcggt cagccagcgc gccacatagc tgcggacaat attggcatgt
    12001 gatttatctt cccgcccttt accatgaata atcagcacat tacgcaaacc atccgccagg
    12061 gcttgttgaa tgaaactgaa caccattttg cggcactctt ccaccggctg gcgcaaaaga
    12121 ttcaggctcg cctgttgcgg atatttacca ctgcgcagct tatccagcac cccatgctgc
    12181 aacccttcct gccgaaactc cagcggctga cttagcggga tgatgtcgag aaatccggta
    12241 gtgaggaaat tatcaagctg cagcgtgtcg atacgctgcg gcgcacgttg gttacgcgtt
    12301 ggatgccagt ggacatctgt agcacgtttc agcggctgga catcttccat ggcgtcaaga
    12361 aacagcgatt tgtcgtcaag gttcatgtaa cctcctatgc accttcacac gtcgtgattc
    12421 agacgggtta attgttcata cttactgcat cgttcccttc acagattgaa acaatactct
    12481 acttcatacc tgtgagtatc tgcgcaggcg gggcatttta tactagcctg ttagcagtat
    12541 cgaacagttt agcccgagtt taattcagca caggctccga tcaggatgat ttattcatag
    12601 caggaaccaa caaaaatgcg ataaataaca gaccactgat tagcagaaag gtaccgaaaa
    12661 tgccatagtt tgcgcctccc tcagcatcaa ttgactcaat aatttggttg aagccagtat
    12721 ttccgatgtc tggtcacttc tactgcgatt aaaaaataaa gcacttctgg aaatgaagtc
    12781 attcaccgta tggggaacag aaaccatttt ttcacccatt ctcgaaaaat gaaaattagt
    12841 tcgattattg agaatagagc cacacagggc aataccaatc gcgcccccaa gattacgtgt
    12901 taaattaaat acaccagagg ctaacttcag ccgttctttt ggaattcccc ccagcgttaa
    12961 tgttacgatg ggggccatcg caaactgctg cgaaatccct ctaattgcct gtggaaacag
    13021 taattcctgc catccccatt cgtgggttat aggggtaaaa agatacattg aaaacacaaa
    13081 tccgcccagc cccgccatca acagccatcg cagatttatt tttttactta accagaagta
    13141 aaagggaaca gaaaataact ggaaaatacc tgttgtgcaa acggcaaaac ctatctcttc
    13201 ggcatttaat ccgcgaatct gccccaaaaa cacaggtatt aaatatacag tagagaatat
    13261 cccaacccct ccagaaaaag aaaaaaaaca tcccagagtg aaatttttat ccttaaaagc
    13321 atgaagatcc attatcggat tggatattgt aagtgtacgc gcagcaaata aaataaaact
    13381 caccaatgcc agaactgatg tcagcaaaat agtattatca tccagccatc cccagcgcgc
    13441 tccttcttca agggtatatt ccagacatcc cagagtcatg gccaacagga taatactcgg
    13501 ataatcagca actttcagta aagagagatc aggtttatca aaattaacca gaaaaggaat
    13561 actcaaaact aaatatatgc caggtaatat attgatataa aatagccatc gccagtcgag
    13621 gttatcagtg agccagcccc ccagagttgg ccctagcgtc ggtgacagtg aggccagcgc
    13681 actgactacc gcagcagcca accccaactc tttaccctgg taataaataa acgccatcgt
    13741 gaaaactaaa gggatcatgg atgcgccagc agctccctgt aaagcacgaa acaaaatcat
    13801 gatctgaata ttccatgcaa gtccacacgc gatactcatt aaagtaaata ttcccgcgga
    13861 tagagtaaaa agccaacgag tggaaaatac ccgtgacagc cacccagaga gcggaataac
    13921 aataatctca gcaattaaat agctggtctg aagccaggcc atttcgtctt tacctgcgga
    13981 cagaccacca cctatttcat ttagggatga cgatacaatt tgaatatcaa gctgagcaat
    14041 aaataatccc atacacatac tggcgaaagc aatatgttta cgcatagaca tcatctctca
    14101 tcaacactga cggtgacgga cagtcccggg cgaagaagtt gctttaattc ctttgcgtcg
    14161 tcaaatgcaa tccgtaccgg gacacgctga acaattttgg tgaaattgcc agtggcattt
    14221 tctatcggaa tcaagctgaa actggctcct gtcgctgggg aaaggctttc aatgtgaccg
    14281 tgaaacactt cccctttgag aatatcagca cggatctcag ccttcattcc tggcttcatt
    14341 cccgctattt ggttttcttt ataatttgca tcaacccaca actcactgac tggtactagt
    14401 gaaactaacg atgtgccgcc ttccacccaa ctgcctgtat gggcgctgcg gtttgcgaca
    14461 atgccatcaa tcggcgca
//
LOCUS       DBKELA010000099        14321 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.099, whole genome shotgun sequence.
ACCESSION   DBKELA010000099 DBKELA010000000
VERSION     DBKELA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.099"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..581
                     /gene="cbrC"
                     /locus_tag="ACUQR0_003154"
     CDS             <1..581
                     /gene="cbrC"
                     /locus_tag="ACUQR0_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HHV9110091.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="ACUQR0_003155"
     CDS             complement(635..1357)
                     /locus_tag="ACUQR0_003155"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HHV9110092.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="ACUQR0_003156"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="ACUQR0_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="ACUQR0_003157"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="ACUQR0_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HHV9110093.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="ACUQR0_003158"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="ACUQR0_003158"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HHV9110094.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="ACUQR0_003159"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="ACUQR0_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HHV9110095.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="ACUQR0_003160"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="ACUQR0_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HHV9110096.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="ACUQR0_003161"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="ACUQR0_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HHV9110097.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="ACUQR0_003162"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="ACUQR0_003162"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HHV9110098.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="ACUQR0_003163"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="ACUQR0_003163"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HHV9110099.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="ACUQR0_003164"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="ACUQR0_003164"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HHV9110100.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="ACUQR0_003165"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="ACUQR0_003165"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HHV9110101.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14321)
                     /gene="lpfD-O113"
                     /locus_tag="ACUQR0_003166"
     CDS             complement(13620..>14321)
                     /gene="lpfD-O113"
                     /locus_tag="ACUQR0_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HHV9110102.1"
                     /translation="GYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKAIVYFYIKK
                     TFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINNLKPVCFD
                     FKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVV
                     TSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAP
                     GQFTATATITVEIVR"
BASE COUNT         3677 a         3671 c         3273 g         3700 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc c
//
LOCUS       DBKELA010000100        14252 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.100, whole genome shotgun sequence.
ACCESSION   DBKELA010000100 DBKELA010000000
VERSION     DBKELA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.100"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..106)
                     /locus_tag="ACUQR0_003167"
     CDS             complement(<1..106)
                     /locus_tag="ACUQR0_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HHV9110103.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="ACUQR0_003168"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="ACUQR0_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HHV9110104.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1355..2005
                     /gene="csgD"
                     /locus_tag="ACUQR0_003169"
     CDS             1355..2005
                     /gene="csgD"
                     /locus_tag="ACUQR0_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HHV9110105.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2010..2399
                     /gene="csgE"
                     /locus_tag="ACUQR0_003170"
     CDS             2010..2399
                     /gene="csgE"
                     /locus_tag="ACUQR0_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HHV9110106.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2424..2840
                     /gene="csgF"
                     /locus_tag="ACUQR0_003171"
     CDS             2424..2840
                     /gene="csgF"
                     /locus_tag="ACUQR0_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HHV9110107.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2867..3642
                     /locus_tag="ACUQR0_003172"
                     /pseudo
     CDS             2867..3642
                     /locus_tag="ACUQR0_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(3706..4197)
                     /locus_tag="ACUQR0_003173"
     CDS             complement(3706..4197)
                     /locus_tag="ACUQR0_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HHV9110108.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4299..4853)
                     /gene="ycdY"
                     /locus_tag="ACUQR0_003174"
     CDS             complement(4299..4853)
                     /gene="ycdY"
                     /locus_tag="ACUQR0_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HHV9110109.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4877..5614)
                     /gene="ycdX"
                     /locus_tag="ACUQR0_003175"
     CDS             complement(4877..5614)
                     /gene="ycdX"
                     /locus_tag="ACUQR0_003175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HHV9110110.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5669..6607)
                     /gene="ghrA"
                     /locus_tag="ACUQR0_003176"
                     /pseudo
     CDS             complement(5669..6607)
                     /gene="ghrA"
                     /locus_tag="ACUQR0_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6842..6929
                     /locus_tag="ACUQR0_003177"
     tRNA            6842..6929
                     /locus_tag="ACUQR0_003177"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            complement(7079..7360)
                     /locus_tag="ACUQR0_003178"
                     /pseudo
     CDS             complement(7079..7360)
                     /locus_tag="ACUQR0_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7642..8646)
                     /locus_tag="ACUQR0_003179"
     CDS             complement(7642..8646)
                     /locus_tag="ACUQR0_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HHV9110111.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8718..9278)
                     /locus_tag="ACUQR0_003180"
                     /pseudo
     CDS             complement(8718..9278)
                     /locus_tag="ACUQR0_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9363..9560)
                     /locus_tag="ACUQR0_003181"
     CDS             complement(9363..9560)
                     /locus_tag="ACUQR0_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HHV9110112.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9572..10063)
                     /locus_tag="ACUQR0_003182"
     CDS             complement(9572..10063)
                     /locus_tag="ACUQR0_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HHV9110113.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10060..10434)
                     /locus_tag="ACUQR0_003183"
     CDS             complement(10060..10434)
                     /locus_tag="ACUQR0_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HHV9110114.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10481..10855)
                     /locus_tag="ACUQR0_003184"
     CDS             complement(10481..10855)
                     /locus_tag="ACUQR0_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HHV9110115.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11018..11239)
                     /locus_tag="ACUQR0_003185"
     CDS             complement(11018..11239)
                     /locus_tag="ACUQR0_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HHV9110116.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11302..11778)
                     /locus_tag="ACUQR0_003186"
     CDS             complement(11302..11778)
                     /locus_tag="ACUQR0_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HHV9110117.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11794..12267)
                     /locus_tag="ACUQR0_003187"
     CDS             complement(11794..12267)
                     /locus_tag="ACUQR0_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HHV9110118.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12609..13430)
                     /locus_tag="ACUQR0_003188"
     CDS             complement(12609..13430)
                     /locus_tag="ACUQR0_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HHV9110119.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13530..13703)
                     /locus_tag="ACUQR0_003189"
     CDS             complement(13530..13703)
                     /locus_tag="ACUQR0_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HHV9110120.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13823..>14252)
                     /locus_tag="ACUQR0_003190"
     CDS             complement(13823..>14252)
                     /locus_tag="ACUQR0_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110121.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3641 a         3490 c         3413 g         3708 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatattttta
     1021 tatgtattat tagtaagtta gcaccatttg tatgattttt taaaattgtg caataaaaac
     1081 caaatgtaca acttttctat catttctaaa cttaataaaa ccttaatgtt aacattttaa
     1141 tataatgagt tacattttgt tacatgttta acacttgatt taagatttgt aatggctaga
     1201 ttgaaatcag atgtaatcca ttagttttat attttaccca tttagggctg atttattact
     1261 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
     1321 aggtgtgcga tcaataaaaa agtggggttt catcatgttt aatgaagtcc atagtattca
     1381 tggtcataca ttattgttga tcactaaacc ttctttgcag gcgacagctc tcttgcagca
     1441 ccttaaacaa tcgctagcaa ttacaggaaa attacataat attcaacgtt ctctggacga
     1501 tatctcttca ggctctatta ttcttctgga tatgatggaa gcggataaaa agcttatcca
     1561 ttattggcag gatactttga gcaggaaaaa caacaatatc aaaatattgt tgctaaatac
     1621 gcctgaagat tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta
     1681 tgccatggag gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gtggcgaatg
     1741 ctactttacg caaaagcttg ccagttacct gattacacat tctggtaact atcgttataa
     1801 cagcacggaa tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat
     1861 cggcgcgtct aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac
     1921 gcatctttat aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg
     1981 ggcaaacgat aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg
     2041 cagaatttct gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgt
     2101 taactgacca tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat
     2161 gggaaagtga ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa
     2221 gctggatcac aataacggtc aatcaggatg ttattttcca gactttttta ttcccgttga
     2281 aaagagactt cgagaaaact gtcgtctttg cactgattca aactgaagaa gcactaaatc
     2341 gtcgccagat aaatcaggcg ttattaagta cggacgattt ggcgcatgat gaattctaaa
     2401 taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta
     2461 tttcgccatt aagttgggct ggaaccatga ctttccagtt ccgtaatcca aactttggtg
     2521 gtaacccaaa taatggcgct tttttattaa atagcgctca ggcccaaaac tcttataaag
     2581 atccgagcta taacgatgac tttggtattg aaacaccctc agcgttagat aactttactc
     2641 aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg
     2701 gccgcatggt gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt
     2761 tgaacgtgac agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa
     2821 ataactcaac cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt
     2881 tcttttggtt gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc
     2941 cagaccgaca ttaatgcctc gtgctcagag ctacaaagat ttgacccatc tgccagcgcc
     3001 gacgggtaag atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc
     3061 ctacccggca agtaacttct ccactgctgt tccgcaaagc gccagggctt acaaaacctg
     3121 cttaacgagc gcaaggttat tcgtgcggca caagaaaacg gcacggttgc cattaataac
     3181 cgaatcccgc tgcaatcttt aacggcggca aatattatgg ttgaaggttc gattatcggt
     3241 tatgaaagca acgttaaatc gggcggtgtt ggggcaagat attttgggat cggtgcagac
     3301 acgcaatacc agctcgatca gattgccgtg aacctgcgtg tcgtcaatgt gagtaccggt
     3361 gagatcctct cctcggtgaa caccagtaag acgatacttt cctatgaagt tcaggccggg
     3421 gttttccgct ttattgacta ccagcgcttg ctggaagggg aagtgggtta cacctcaaac
     3481 gaacccgtta tgctgtgtct gatgtcggct atcgaaacag gggtcatttt cctgattaat
     3541 gatggtatcg accgtggtct gtgggatttg taaaataaag cagaacggca gaatgacatt
     3601 ctggtgaaat accgccatat gtcggttcca ccggaatcct gacagataaa aatgccggaa
     3661 ttaataatcc ggcatttact gtttaagcga aagcgtgagt gcccctcacg ctttattttt
     3721 gatttcctgc tcacggtgag ccgtctttgc actacgcgcc gccagccaca ggccgctgtt
     3781 tttcattgcg taaccaaata ccagcccaag cgccagcgaa ggtaacacca gtttccagtc
     3841 accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc cccggaacaa atgaaagcag
     3901 cagctgtttg gcctgaatac acatcagaaa cgcgacaata ccggttatga cataaccgag
     3961 aatttccaga tgtggtgcca gtgcactacc gtaaataatg accatcgccc acaccacgcc
     4021 actaagcagg gttgcagcgg agatcgccag ccctttcagc ccaccttgcg ggcaagcaaa
     4081 gtaggccgta cagcccagga agcccgccca gcttagtaag cccagggaaa cagccaccca
     4141 tccccagata ccggagagaa tgcctgttgt gattgcaata gagagaagta tgttcatgcc
     4201 gcgcatgata gcagaaaacg cggccatgaa tgagatcgag cacacatttt atgcaacaac
     4261 aaattatctg ttgcagttat gagtgacgat gatcacattt actcttcaga atcttcttcc
     4321 agctcgtccc acattgcact aatggcatca cgggttagcg gtgccatggt gcgccagaaa
     4381 ggcgtggttg catgggcctc cactttgccg aggaacgcac cacaccaggg taacagatac
     4441 tcactgaaca gtgtttccag tgcttcgctc tcatcttccg ttgactgatc ttccagccag
     4501 gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg tatccgccag tggcatccct
     4561 cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt cctcaaccca tgcgctacga
     4621 tatggcggca cggcacattc atcgccgata aacaacgcat tgtaatcggc agagacttgc
     4681 gtcatatcac agcttttctg taaacgcgtc agtaactcat cctgctccag tggccagttc
     4741 gcagccagtt tcccctcacg aatcagagta aacagcggca ccagtaaagg atcttgcggt
     4801 tggcggtaat agagcgaacc cagcacacga cagaggatag aaaactcgtt cattaatata
     4861 tttccattac tagtaattaa aggtctgcaa attccgcaat cggtgccata ccgcgagatt
     4921 caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat gcgctctagc ggaaaatcta
     4981 ccgcgtcgag gattttaaga cactcttcaa attcccccat ggtaaacgcg gtgtgagaat
     5041 ccgagcctaa tgccacccaa cctccagcat cacgtaccgc tgcagctacc gcacgacagt
     5101 tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt attgatttcc agcgccacct
     5161 gatgtttcgc ggctgcttca gcaacggctt taacatctat ttcatatttc ggatttccgg
     5221 gatggcttat tatatgcaca ttgccgctgg cgatagtgga gatcatcgct tgtgtattgg
     5281 tcgctttgtc atgtggcgca aaaaccggct catgaaaacc ggcaataatc agatccagcg
     5341 agtcaaacat tttaccgctg cagtcaattt caccatcaac atttttaatg ttagcttcaa
     5401 tgccgcgcag gatccctacc ccatcaacca ctcgcggcca gatacgcatg ttaatgaagt
     5461 gccagtgatg cggcgcatct tccatatccg ggccatgatc ggtgatcgca aaaagtttaa
     5521 tgcccttttg tttggcctgg gcaatgtaat cactcaatgt gctatatgca tgtgtgctgg
     5581 caacggtatg catatgaagg tcgacgggat acataactct ctcctgtatt caattcccgc
     5641 caaggatagc aggaatcctg atgctttatt agtagccacg tgcgcggtcg acttgcccgc
     5701 agaccctctc ccctttttcg agctgggcaa tagtgcgaga aatgtactcc acagcttcag
     5761 cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac gcgtggatgt tgccagagcg
     5821 gactttcagg cggtaagggt tcacgattaa aaacatccag cattgcgcct ttaactttcc
     5881 cgctatccag cgccgcgagc aggtcatctt ccacaacatg aacaccacgc gccaggttga
     5941 ggagatacgc gccatcaggt aatttttcga gtaattgttg attaataatg ccgacggttt
     6001 caggggtatt cggtaacaaa ttaatcaata cccgacattg gctcagaaat gcagacagtt
     6061 cttcccgtcc ggcaaagctt tgcacgccag gccacgattt acgggttcga ctccagcaac
     6121 gcagcggaaa gcgctaggtt tgcagactct gagcaacttt acagcccagt acgcctgcgc
     6181 ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg cagcggttgc caatgcgaac
     6241 tattttgctg gatgcgataa tcgtcaaaac gtcgaaacca atgcagcacc tgactgacag
     6301 catattcctg catttgctcg cccataccgg tatcttccag gcgaaaaagt ggaacagaag
     6361 ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat agaatcaaca ccggccccga
     6421 gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc aacaggagga tgccagacta
     6481 aaacataatc agcagagtca ttatctccgc ttttccatgc cctgactctt gcctgaggaa
     6541 tagctttgcg cagtgcctca atccaccatt gggtatcgaa cgttgggtga taaaagatga
     6601 tatccatact gactcccgaa aagcgttgtg cgaaatttat tcgcacttat cgttatgatc
     6661 tacaaaggcc accagcataa caaatccgtg gtcggtggca aaaaaagcag atttcgctta
     6721 ttaaaaccac acattgattg aatgtttgaa taaacgcgcg attttttcaa aaagtttgtt
     6781 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     6841 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     6901 cgggggttcg aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc
     6961 gcttttttat ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta
     7021 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     7081 atactgccag catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     7141 ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
     7201 catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga
     7261 tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat
     7321 cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt
     7381 tgcaacactg atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg
     7441 caaggatgcc cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt
     7501 cagtgagcca tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa
     7561 taatcagtct tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat
     7621 tgagttatat cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca
     7681 ggcaattcga gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt
     7741 gagttgctcc cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa
     7801 tgagcgggca cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc
     7861 caacagagtc gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc
     7921 aaaatccctg ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc
     7981 agtaatcgga ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat
     8041 tgactgactt aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt
     8101 atgcaacctc accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag
     8161 agacaaccgt tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa
     8221 accgtggatc tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg
     8281 taacgccctc atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg
     8341 tgaagccgct tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa
     8401 cgggcgaacg aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg
     8461 agccataaag tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc
     8521 cagaaactgc atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt
     8581 gtcttgacaa tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt
     8641 ctgcatgatg gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg
     8701 gggtgaccat atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta
     8761 ctgccaaatt tacaggggca attggttttg taattccagt tcagccctct gaagacgttg
     8821 atcacccctc gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga
     8881 atgttcgctt cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca
     8941 cgggcctgcg tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt
     9001 tcgcgatcaa gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag
     9061 cagccgcagc gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt
     9121 tccgtcagca ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta
     9181 ctctttcgta cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg
     9241 ttgaccactt ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga
     9301 taaatgaaaa cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag
     9361 cttcaccggt tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc
     9421 tgttcgatac agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata
     9481 attcccgatc cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt
     9541 tccgtggtta atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca
     9601 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga
     9661 cgaccagacg acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg
     9721 accacgcatc ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac
     9781 cgaagtcact ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat
     9841 gtgcggtact gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg
     9901 tctggtcaga ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca
     9961 gttcagcgag ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta
    10021 cgttaacgct gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt
    10081 acccagggta atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg
    10141 gcgagcccgg aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc
    10201 cggctggtcg gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag
    10261 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt
    10321 cagtgtgagg ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac
    10381 gggagacggg cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag
    10441 ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg
    10501 ctggtgtcgg ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc
    10561 aggtcagccc ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc
    10621 tggtgagcat gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
    10681 catctgcgtc gctgaagcgg cctctgatac cggcgcggtc ggcaaggtaa tacaggcggt
    10741 taccctcttg caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc
    10801 aggggcggtc gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa
    10861 aatcctctct gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga
    10921 ccggtttcgg tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag
    10981 gtcatctggt cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca
    11041 gccggacata cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg
    11101 gaatatcctg cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac
    11161 cggtacagaa cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac
    11221 cacgggtgat gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag
    11281 gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt
    11341 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    11401 acgttcggtg ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg
    11461 cgccagcacc acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg
    11521 aacttccgta cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg
    11581 gttgttcaga tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag
    11641 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    11701 cagggtttgc agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag
    11761 aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
    11821 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
    11881 acactccgta cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc
    11941 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc
    12001 atcatcgtca tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt
    12061 ccagatacca ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc
    12121 cagggtgacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc
    12181 atcagggacg ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc
    12241 ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag
    12301 ggagtaaagg tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat
    12361 atcagggatg aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt
    12421 cacagacgga tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc
    12481 gggacggtaa agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca
    12541 ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac
    12601 atgctgcctc agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg
    12661 agcttaatat cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca
    12721 ctgcggccgg aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac
    12781 aggtctttac cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc
    12841 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
    12901 ggcggcagga ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc
    12961 accacctcgt aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc
    13021 aattctccca gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg
    13081 ggtaacatct ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca
    13141 tgctgaccgg ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg
    13201 cgacccgggt cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc
    13261 aggctttcca gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc
    13321 cggtcttcac cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg
    13381 tcacggcgta tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc
    13441 ttatcggtgg ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga
    13501 cgcggcctga tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac
    13561 cttccggcgt cagtctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg
    13621 tcgataaaca cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg
    13681 acttcgggca ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag
    13741 tccctgtcat catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag
    13801 atgaaccgca taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg
    13861 cgaattcgtg aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat
    13921 agtaaccaac gactttatgc gtttttgggt tatagagtgt tacgatatag tcccagccgc
    13981 aggcgtgggc tttacaaccg ccaccttcta cccaggtcgt tccgttaatg tttacaggat
    14041 acgcaccacc atcaggacca tccatatagc cgtcttcccc aaacattccg tttaacccaa
    14101 cagggtttaa tgctttgtcg atagcagctc tgtaggtagc gtcaccttcg tataactcgt
    14161 acgttgttgg acatgcacca ggctggtcag cccgggcttt tgagtcgcat ttaagtgtct
    14221 gatttacagt ggcggcgttt gtgtcaggac tt
//
LOCUS       DBKELA010000101        13963 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.101, whole genome shotgun sequence.
ACCESSION   DBKELA010000101 DBKELA010000000
VERSION     DBKELA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.101"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACUQR0_003191"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACUQR0_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHV9110122.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACUQR0_003192"
     CDS             2219..2767
                     /locus_tag="ACUQR0_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HHV9110123.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACUQR0_003193"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACUQR0_003193"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HHV9110124.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACUQR0_003194"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACUQR0_003194"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HHV9110125.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACUQR0_003195"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACUQR0_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HHV9110126.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACUQR0_003196"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACUQR0_003196"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HHV9110127.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACUQR0_003197"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACUQR0_003197"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HHV9110128.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="ACUQR0_003198"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="ACUQR0_003198"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HHV9110129.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACUQR0_003199"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACUQR0_003199"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HHV9110130.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="ACUQR0_003200"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="ACUQR0_003200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HHV9110131.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3562 c         3525 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DBKELA010000102        13955 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.102, whole genome shotgun sequence.
ACCESSION   DBKELA010000102 DBKELA010000000
VERSION     DBKELA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.102"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            295..1548
                     /gene="kch"
                     /locus_tag="ACUQR0_003201"
     CDS             295..1548
                     /gene="kch"
                     /locus_tag="ACUQR0_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HHV9110132.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1603..1776)
                     /locus_tag="ACUQR0_003202"
     CDS             complement(1603..1776)
                     /locus_tag="ACUQR0_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HHV9110133.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1919..3379
                     /gene="cls"
                     /locus_tag="ACUQR0_003203"
     CDS             1919..3379
                     /gene="cls"
                     /locus_tag="ACUQR0_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HHV9110134.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3414..3743
                     /locus_tag="ACUQR0_003204"
     CDS             3414..3743
                     /locus_tag="ACUQR0_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HHV9110135.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3796..4800)
                     /gene="oppF"
                     /locus_tag="ACUQR0_003205"
     CDS             complement(3796..4800)
                     /gene="oppF"
                     /locus_tag="ACUQR0_003205"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HHV9110136.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4797..5810)
                     /gene="oppD"
                     /locus_tag="ACUQR0_003206"
     CDS             complement(4797..5810)
                     /gene="oppD"
                     /locus_tag="ACUQR0_003206"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HHV9110137.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5822..6730)
                     /gene="oppC"
                     /locus_tag="ACUQR0_003207"
     CDS             complement(5822..6730)
                     /gene="oppC"
                     /locus_tag="ACUQR0_003207"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HHV9110138.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6745..7665)
                     /gene="oppB"
                     /locus_tag="ACUQR0_003208"
     CDS             complement(6745..7665)
                     /gene="oppB"
                     /locus_tag="ACUQR0_003208"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HHV9110139.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7751..9382)
                     /gene="oppA"
                     /locus_tag="ACUQR0_003209"
     CDS             complement(7751..9382)
                     /gene="oppA"
                     /locus_tag="ACUQR0_003209"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HHV9110140.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10120..10767)
                     /gene="ychE"
                     /locus_tag="ACUQR0_003210"
     CDS             complement(10120..10767)
                     /gene="ychE"
                     /locus_tag="ACUQR0_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HHV9110141.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(10798..10851)
                     /gene="ychE"
                     /locus_tag="ACUQR0_003211"
     CDS             complement(10798..10851)
                     /gene="ychE"
                     /locus_tag="ACUQR0_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HHV9110142.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            11244..13919
                     /gene="adhE"
                     /locus_tag="ACUQR0_003212"
                     /gene_synonym="adhC"
     CDS             11244..13919
                     /gene="adhE"
                     /locus_tag="ACUQR0_003212"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HHV9110143.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3660 a         3454 c         3244 g         3597 t
ORIGIN      
        1 gatccaatat ttaagggcac ggtttttgca accgtgttct tgtttggcct ttccttatag
       61 ccggtcagtg cattcactac cacaagcaga taataacttt atctgtattt ctttatatga
      121 tgtgagcatg ttcaggaaaa aagccctttc ctgacgcaga gttgctcact caaaaccttt
      181 gattactgcg gataaagtca tctactattg tttaacacat tcacgcgctg catgtatgtt
      241 cagaaaatgt gcacgctaag ataattaaac aaaattgtgg ttacagaggt atcagtgagt
      301 cactgggcta cattcaaaca aacagcaaca aatttatggg tgaccctacg gcacgacatt
      361 ctcgcgctgg ccgtcttttt aaatggattg cttattttta aaacaatcta tggtatgtcg
      421 gtcaatttgc ttgatatttt ccatatcaaa gcattttcag agttggatct ctccttgctg
      481 gcaaacgccc cactatttat gctcggcgtc tttcttgtcc tgaactccat tggcttactg
      541 ttccgggcaa agctcgcatg ggcaatcagt atcattttgt tgttgatagc gctaatttac
      601 accctgcatt tttatccctg gctgaaattt agtattggat tttgcatttt tacgctggtg
      661 tttttgctga tactgcgcaa agacttctcc cacagtagcg ccgcagccgg gacaattttt
      721 gcatttatta gtttcacgac attactgttt tactcaacct acggtgcgct ttatttaagc
      781 gaaggtttta atccgcgaat agaaagtttg atgaccgcgt tctatttttc gatagaaacc
      841 atgtcaaccg tcggctatgg agatattgtc cctgtttctg aatcagcacg attgttcact
      901 atttcggtca ttatttccgg cattaccgtt tttgccacat ctatgacctc aatttttggt
      961 ccgcttatcc gcgggggatt caacaaactt ttaaaaggaa acaatcatac aatgcatcgt
     1021 aaagatcatt ttattgtttg cggacattcg attctcgcca tcaatacgat cctgcaactg
     1081 aatcaacgcg gacaaaacgt aacggttatc agcaacttgc ctgaagatga tatcaagcaa
     1141 cttgagcaac gcttaggcga taacgctgat gttatccccg gtgacagtaa tgacagttca
     1201 gtattaaaga aagcgggaat cgatcgttgc cgggccattc tggcgctgag tgataacgat
     1261 gcagataacg cgtttgttgt actctcggca aaagatatga gcagtgatgt caaaacagtt
     1321 ctcgccgtca gtgatagcaa aaacctgaat aagattaaga tggtacatcc ggatatcatt
     1381 ctctcaccgc aactgtttgg cagcgaaatt ctggcgcgag ttttaaatgg tgaagagatt
     1441 aataatgata tgctcgtttc aatgttgttg aactcaggtc atggtatttt cagcgataac
     1501 gatgaacaag aaacgaaagc tgacagtaaa gaatcggcgc aaaaatagag tttaaagacg
     1561 gaagagtaaa cttattgggc accgcaatgg tgcccagtac atttagatat tgtagatcgc
     1621 aaacaataac gagttacgct gtttgtttag aatgcacttc ctgatgctgt ggatacgcat
     1681 atttcggcgc gattgcccct caagccaacg cgatttacgt cggctagcct gacgctgcat
     1741 gcgccagcgc cccacttccg ttctactccg cttcatgtta actactctat gcaataacag
     1801 aatggtcatt atacccccgc ccccgacgct gaccagtgct tttcccgcgt ttttatttgc
     1861 cagatgaatc catatgcgta aactcataac aatgcgcttt caaaaggatt tctaatctat
     1921 gacaaccgtt tatacgttgg tgagttggtt ggccattctg ggatactggt tgctcattgc
     1981 aggcgtaact ttacgcattc taatgaaacg acgcgcagtt ccctccgcga tggcctggct
     2041 gttgattatt tacattctgc cgttagtcgg aattattgcc tatcttgccg ttggcgagct
     2101 ccatttaggc aaacgccgcg ctgagcgcgc cagagcgatg tggccttcca ccgcaaaatg
     2161 gcttaacgac cttaaagcct gtaagcatat cttcgccgaa gaaaatagca gtgtcgctgc
     2221 gccattattc aagctttgcg agcgtcgtca ggggatcgct ggggtcaaag ggaatcagct
     2281 acagctgatg accgagtcag atgatgtgat gcaggcgtta atccgcgaca tccagctcgc
     2341 gcgccataat attgagatgg tgttttatat ctggcagccc ggcggcatgg cggaccaggt
     2401 ggctgaatca ttaatggcgg ctgcacggcg tggtattcat tgccgattga tgctcgactc
     2461 cgccgggagt gtggcttttt tccgcagccc gtggcccgag ctaatgcgta atgccggtat
     2521 tgaagtggtc gaagccttaa aggtcaatct gatgcgtgtg tttttacgcc gtatggacct
     2581 gcgccaacat cgcaagatga tcatgatcga taattacatc gcgtacaccg gcagcatgaa
     2641 tatggtcgat cctcgctact tcaaacaaga tgcgggcgta gggcaatgga ttgatctgat
     2701 ggcgcgtatg gaaggcccca tcgccaccgc gatggggatt atttattcct gcgactggga
     2761 gattgaaacc ggaaaacgta ttctgccgcc accaccagat gtcaatatta tgccgtttga
     2821 acaggccagc ggtcacacca ttcacacaat tgcttctggc cccggctttc cggaagatct
     2881 cattcaccag gcattattga ctgcggctta ttcggcgcat gaatatttga tcatgaccac
     2941 gccctacttt gtgccaagcg atgatttact tcatgcgatt tgcacggcgg cgcagcgcgg
     3001 ggtggatgtc agtattatcc ttccgcgaaa aaatgactcg atgctggtcg gctgggccag
     3061 tcgcgcattc tttacggaac tgcttgctgc tggggttaaa atttatcagt ttgaaggcgg
     3121 gttactgcat accaagagcg tgctggtcga tggcgaacta agtctggttg gcaccgttaa
     3181 ccttgatatg cgtagtctgt ggctgaattt cgagattacc ttggcaatcg acgataaagg
     3241 ttttggtgca gaccttgccg ccgttcagga cgattatatt tcgcgttcac gtctgctcga
     3301 tgcccgttta tggctaaaac gtccattatg gcaacgtgtc gccgagcgac tgttttactt
     3361 cttcagcccg ttgctgtaaa acgtcgccaa cagaggttaa acaggtagtg actatggata
     3421 tggatctaaa caatcgcctg actgaagatg aaacgcttga gcaggcctac gatatttttc
     3481 tcgaactggc tgccgacaat ctcgatccag ctgatgttct gctgttcaat cttcagtttg
     3541 aagagcgcgg cggcgcggag ttattcgatc cggcggaaga ctggcaggaa catgttgatt
     3601 tcgatttgaa cccggacttt tttgccgaag tggtgattgg tctggcggac agtgaagatg
     3661 gtgaaatcaa cgatgttttc gcccgcattc tgttatgccg cgaaaaagat cacaaacttt
     3721 gccatatcat ctggcgggaa taaataatcg cttttagcgt gaaaaagggc tgacaactgt
     3781 cagcccttat tgtttttaaa gcggatcgac tttcaggcaa gaaacggcgt gtctgaaact
     3841 cccctccaga acaggacgtg ttttggcgca ctccggaccg gcaatcgggc aacgggtacg
     3901 gaaaacacaa ccggaaggcg ggttgatcgg cgacggtaat tccccttcca gtaactggat
     3961 ggttttgttc ttctccagat ccggatcagg tatggggact gccgacatca atgccctggt
     4021 gtaaggatgt agtggattgt ggtagacctc atcataggtc cccagttcta ctgcatggcc
     4081 gagatacatc accaacacac gatcggaaat gtgttttacc acggccaagt catgagcaat
     4141 aaaaattaat gacaatccca tctcacgttg cagctgctgg agcaggttaa ccacctgcgc
     4201 ctgaattgac acgtccagcg ccgaaaccgg ctcatcgcag ataatcagct tcggttcaag
     4261 aataagagca cgagcaatcc cgatacgctg gcactgccca ccggagaact catgcggata
     4321 gcggttaatc aggttaggca ataacccgac tttcagcatc atcgccttca cgcgctcgcg
     4381 aacttcctgg cgtgacattt tcggatgata agtacgcagt ggttcagcga tgatctcgcc
     4441 gatggtcata cgcgggttta gcgatgccaa cggatcctgg aaaatcatct gaatatcact
     4501 gcgaacggca cgccattcat cgggcttcat gcccagcaac tctttaccta accaggcaac
     4561 atgaccgtcg gtcgccttga ccaaaccgat gatggcgcga gcaaaggtgg acttaccgca
     4621 tcccgattcc cctaccacac ctaatgtttc cccttcatac aggcgaagag tgacaccatc
     4681 gacggctttg agcgttttcg gcggttgcca gaaccactgt ttgccatctt tgatttcaaa
     4741 gtgcacttta agatcggcga tttcaaggag gacttttctt ccttcagtta cagcattcat
     4801 aacagttcct ccaccggttt aaagcaagca cgcagacggc caggcgtaaa ctcttccagc
     4861 ggcggtgcgc tactacaaat ttccatcgca tgtggacaac gtggctggaa cgggcaacct
     4921 ttcggtaatc gcagcaagtt tggcggatta cccgggatgg tcaacattgt ttcaccttcc
     4981 gcatcaagac gcggcaccgc gttgagcaaa ccgatagaat aaggatgaac gggttgataa
     5041 aagacatcgc gagcattgcc atattccatc gtgcgcccgg cgtacattac cagcactttg
     5101 tcacagatcc ccgccaccac gccaagatcg tgggtgatca taatgatggc agtattaaat
     5161 tcccgcttca gttcattcaa tagcgtcatg atctgcgcct gtacggtgac gtccagcgct
     5221 gtagtgggtt catccgcaat cagcaactta ggtcgacata gcaatgccat cgcaatcatg
     5281 actcgctgac gcatgccgcc agaaaattcg tgcgggtaca ttttcatgcg tttacgtgct
     5341 tccggcattt ttaccgcatc gagcatccgc accgactctt caaacgcctc agctttgctc
     5401 atgttcttat gcagcatcag cacttccatc aactgctcac cgacgcgcat atagggattc
     5461 aacgaagtca ttgggtcctg aaaaatcatt gagatttgtt cagcgcgcag tttattgagt
     5521 tcacgctctg gcaaattgag gatttcacgc ccattgaagg ttgccgatcc gccaatgcgt
     5581 ccgttggcag ccaacaggcc catcaacgca aatgcagttt gcgatttacc cgaaccagac
     5641 tcacctacaa tgcccagcgt ttctccggca cgtagggaaa aatttaaatc attgaccgcc
     5701 gtgacgtcgc cgtccggggt actaaaggtg acacgcaaat ctttcacgtt cagcagtgcg
     5761 tcagcctgtt gttgtgcgag cggcacagtt gcagtttcaa ttacgctcat ggctgcactc
     5821 cttaacgatc tttcgggtcg agggcatcac gcaagccatc gccgataaag ttgaaacaaa
     5881 acagtgtcac cacaaggaat cccgctggga acaacagtaa ccatggagag acttccatcg
     5941 agttcgcgcc atcactcagc aatgcccccc agctgcttaa cggctcttgc gtacccaacc
     6001 ccaggaagct aaggaaagat tcaaagagga tcatgctggg caccagtagc gatgcgtaga
     6061 ccaccaccac accgagtacg ttcggcacaa tgtggcgaat aacaatgccc ggcgtcgata
     6121 caccgccaac ttgtgccgcc tcaataaact ctttgcgctt cagactcagg gtttgcccac
     6181 gcacaatacg agccatatcc agccaggaaa ccatgccaat cgccacgaaa atcagcagga
     6241 tgttttggcc gaaaaaggtc accagcaaaa tgacgaagaa catgaatggg aaggagttga
     6301 ggatttccag cagacgcatc attaccgaat ccactttacc gcccagataa ccggaaagcg
     6361 aaccataaag tgtccccacg accactgcca ccagtgccgc agcaacaccg accatgagtg
     6421 agatacgccc gccaatcgca acgcgcacaa gcaggtcgcg accggatgag tcagtaccaa
     6481 agtagtgacc ggactccata tccggggcgc tggacatcat cgcccagtca gtatcgtcat
     6541 aggcaaactg ggaaagcatc ggtgccagga ttacaaataa cgcgatcagc accagcacta
     6601 tcagactggc aaccgccgca cggttatgca taaaacgtcg acgtgcgtcc tgccacaagc
     6661 tgcgcccttc gacctccagc ttttcactga aattttccag cgtctcgctg tttttcttac
     6721 ttaacatcat cgcgagctcc agtatcagta acggattttc gggtcgataa ccgcatatag
     6781 cacatcgaca atggcattaa acaaaatggt taacgcacca accaggatgg tcaggcttaa
     6841 cactaaggaa tagtcacggt tcaatgcgcc attaacgaac agttgcccaa tccccggcaa
     6901 accataaatg gtttcgatga ccatagaacc ggtaataatg ccgacaaatg cggggcccat
     6961 ataggagagc acgggtaaca gagcaggttt taatgcgtgg cgtaaaatga tccgccgcat
     7021 aggtaaccct ttcgcccggg cagtacgaat aaagttggag tgtaatactt caatcataga
     7081 gccacgggta atacgcgcaa tactggcgat ataggcgagt gacaacgcca ccatcggcaa
     7141 tatcatgaat ttaagcgccc caccattcca gccaccgccc ggcagccaat gcaaaatgat
     7201 cgcaaatatc atgactaata atggcgcaac cacaaaactg gggataacaa ccccggtcat
     7261 tgccagcccc atcacggtat agtcccattt ggtgttttgt tttaatgcgg caataacgcc
     7321 agcactaaca cccagtatta ccgcaaggaa aaatgcggcg gctcccagtt tggcagaaac
     7381 gggaaaactg gatgccacca ggtcattgac cgaataatct ttatatttaa acgatggacc
     7441 gaaatcgcca tgcgccagtt gtttcaggta gctgaaatac tgtgtcatga tcggatcatt
     7501 aagatgatat ttcgcttcga tattggccat cacttctggc ggtaaagtac gttcgccggt
     7561 aaaagggctt cccggcgcga ggcgcatcat aaagaacgaa atagtaataa gaataaatag
     7621 cgtcggaatc gcttccagac aacgacgtag aataaatttt aacattgccc taccttctgg
     7681 cctgtgcctt ctgtaatgcg ataaaaatca gacaccgtgg agcaggacac tcctgcccca
     7741 cgtattgcca ttagtgcttc acaatgtaca tattccgggt ataggtatta tccagcggat
     7801 ctttgccggt atagccacca acccacggtt tcaccagacg cgcattcacg tagtaataaa
     7861 caggaacaat ggccgaatcc ttatccagct gttgttctgc tttagtgtac agagctgtgc
     7921 gctgcgcctc gtcagtcact ttcagcgttt ccgccataat gctgtcaaag gccgggctct
     7981 tataatgcgc ggtattcatc gagctgttcg acagcatggt gttcaggaag gaagttggtt
     8041 cgttgtagtc tgcacaccag cctgcacggg ccacatcaaa agtgccctgg tgacgggtgt
     8101 cgaggaacgt tttccactcc tggttaacca gtttgacgtt taccccaatg tttttcttcc
     8161 acaatgaaga ggcagcaatc gccagctttt tatgcagatc ggaggtgtta taccgcaggt
     8221 tgatggtcaa cggtttgtct gcggtataac ccgcttcagc cagcagtttt ttcgcttctt
     8281 cgttacgttt ttcctggctc cagccaaacc attctggctg agtcaatttt gcgccatcag
     8341 tatacggtgg agtataacca taggcgggca tgtcgccctg cgctttcact ttattaacaa
     8401 tgatatcgcg gtccatacct agtttcagtg cggtacgcac acgcacatcg ttgaatggcg
     8461 gtttctggtt gttaatttcg taatagtaag tgcacaggta tggatcaacg tgaacttcgt
     8521 ccgggatctc ttttttcagc ttctggaaca attcgatcgg catgttgtta tacgtcatgt
     8581 cgatttcacc actacggtag cggttgacat cggtaacttc agaagcaata ggcaaatagg
     8641 ttacctggtt aataacggtt ttcgcgttgt tccagtaggt cgggctgcgt tcaagaacga
     8701 ttcgttcgtt tacgacccaa tcttttaagg tataggcacc gttggtgacg atattaccag
     8761 gctgggtcca tttttcgccg aatttttcga tagcggcttt tggcaccggt gaagttgatg
     8821 ggtgaacaag taatttatag aagtacggaa cgggttcact taaggtgact tctaatgtgt
     8881 gatcatcaat agctttcacg ccgagatcgg taatcggttt tttcccttca agaatttcat
     8941 caataccggc gatatgccca tattgcagat aactggcata cggagaagca gtgttcggat
     9001 caacagaacg ttgccagcta tacacaaagt cttgtgctgt gactggcgtg ccatcagacc
     9061 atttcgcatc tttacgcaaa tggaaggtcc agactttcgc gtctttatta tcccaggatt
     9121 cagcgacgcc aggtgctgga tgaccgtcaa gatcgctgac cagtaagcct tcaaacaggt
     9181 ctcggctgat attagactcc ggaacacctt caattttgtg cggatctaat gactgaactt
     9241 ctgaaccatt gttacgtacc agtgtttgtt tttccgccag tgtgacgcct gcgggtacat
     9301 cagctgccag cgcgacattc cctgccatta gcgcagccag aacgccagct gctactaaac
     9361 ttctcttggt gatgttggtc attgtttttt ggactccctc attataatta ctggtattaa
     9421 ccagcatgtg taatcccgga ttgggaccct gcacaagctc ggagactttc tgctgtcagg
     9481 ttactttatc gttacgtatt actatcaccg actttattta ttctggtgat tcagacgatc
     9541 accttatgtc gattcgtact gtgtctcccc tgtcgagaca cattattctt tcgcgacgct
     9601 ctttaattgt taattattct cattaccaat aaagaatcgt ctggcggttg cccggaaagt
     9661 accaaatgcg gttcttactc gccaatacat tttgcaaaat tgttaagcga ttctctttta
     9721 ttgaccgtat cagaatctat agcttgcatc catgccttaa caaacatctt tcaattcaat
     9781 caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt agtgaaatgc
     9841 acaataattt aacaattgat tattatttag cactttcctc taccaaaggc agtggaatta
     9901 ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg aaaataacat
     9961 gatggtgagg agaaacagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
    10021 ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa
    10081 gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa
    10141 aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat
    10201 aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat
    10261 gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
    10321 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
    10381 cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg cggtttctga
    10441 tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat
    10501 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
    10561 tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc
    10621 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
    10681 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
    10741 gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg gataatgtta
    10801 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
    10861 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
    10921 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
    10981 ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg
    11041 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
    11101 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcatcggtta
    11161 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
    11221 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
    11281 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
    11341 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
    11401 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
    11461 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
    11521 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
    11581 ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg
    11641 ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg gctgatatcg
    11701 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
    11761 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
    11821 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
    11881 gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat
    11941 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
    12001 ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc
    12061 tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat ggtgcgctga
    12121 acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
    12181 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
    12241 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
    12301 tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca
    12361 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacagctc
    12421 gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac
    12481 tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg
    12541 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
    12601 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
    12661 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
    12721 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct
    12781 tcttcgaagt agaagcagac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
    12841 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
    12901 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
    12961 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
    13021 tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
    13081 acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga
    13141 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
    13201 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
    13261 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag
    13321 ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc gcgggtatcg
    13381 catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt
    13441 tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg
    13501 cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc
    13561 gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac cgtactgctg
    13621 ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg ggtattccga
    13681 aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt
    13741 ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg ctgatctccg
    13801 agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa ggtgaaactg
    13861 cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat
    13921 cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DBKELA010000103        13799 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.103, whole genome shotgun sequence.
ACCESSION   DBKELA010000103 DBKELA010000000
VERSION     DBKELA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.103"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..815)
                     /locus_tag="ACUQR0_003213"
     CDS             complement(<1..815)
                     /locus_tag="ACUQR0_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HHV9110144.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACUQR0_003214"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACUQR0_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HHV9110145.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACUQR0_003215"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACUQR0_003215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HHV9110146.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACUQR0_003216"
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACUQR0_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HHV9110147.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACUQR0_003217"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACUQR0_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HHV9110148.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACUQR0_003218"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACUQR0_003218"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HHV9110149.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACUQR0_003219"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACUQR0_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HHV9110150.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="ACUQR0_003220"
     CDS             7489..7638
                     /locus_tag="ACUQR0_003220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110151.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="ACUQR0_003221"
     CDS             7839..9422
                     /locus_tag="ACUQR0_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HHV9110152.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="ACUQR0_003222"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="ACUQR0_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HHV9110153.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="ACUQR0_003223"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="ACUQR0_003223"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HHV9110154.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="ACUQR0_003224"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="ACUQR0_003224"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HHV9110155.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..>13799
                     /locus_tag="ACUQR0_003225"
     CDS             13381..>13799
                     /locus_tag="ACUQR0_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HHV9110156.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGG"
BASE COUNT         3192 a         3640 c         3496 g         3471 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagtatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctggcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgagtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
    13681 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
    13741 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttgggggg
//
LOCUS       DBKELA010000104        13522 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.104, whole genome shotgun sequence.
ACCESSION   DBKELA010000104 DBKELA010000000
VERSION     DBKELA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.104"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACUQR0_003226"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACUQR0_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HHV9110157.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACUQR0_003227"
     CDS             1131..1931
                     /locus_tag="ACUQR0_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HHV9110158.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACUQR0_003228"
     CDS             2009..2779
                     /locus_tag="ACUQR0_003228"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHV9110159.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="ACUQR0_003229"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="ACUQR0_003229"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HHV9110160.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="ACUQR0_003230"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="ACUQR0_003230"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HHV9110161.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="ACUQR0_003231"
     CDS             5046..5768
                     /locus_tag="ACUQR0_003231"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHV9110162.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="ACUQR0_003232"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="ACUQR0_003232"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HHV9110163.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="ACUQR0_003233"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="ACUQR0_003233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HHV9110164.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="ACUQR0_003234"
     tRNA            7161..7237
                     /locus_tag="ACUQR0_003234"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="ACUQR0_003235"
     CDS             complement(7368..8414)
                     /locus_tag="ACUQR0_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HHV9110165.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="ACUQR0_003236"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="ACUQR0_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="ACUQR0_003237"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="ACUQR0_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HHV9110166.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="ACUQR0_003238"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="ACUQR0_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HHV9110167.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="ACUQR0_003239"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="ACUQR0_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12614..13522)
                     /gene="tagK"
                     /locus_tag="ACUQR0_003240"
                     /pseudo
     CDS             complement(12614..13522)
                     /gene="tagK"
                     /locus_tag="ACUQR0_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103253951.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3100 a         3521 c         3609 g         3292 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccgtccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct gaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt gg
//
LOCUS       DBKELA010000105        12963 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.105, whole genome shotgun sequence.
ACCESSION   DBKELA010000105 DBKELA010000000
VERSION     DBKELA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.105"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..74
                     /locus_tag="ACUQR0_003241"
     CDS             <1..74
                     /locus_tag="ACUQR0_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HHV9110168.1"
                     /translation="SFSKSVELHDKVIGHYLNIKHYQ"
     gene            90..1016
                     /locus_tag="ACUQR0_003242"
                     /pseudo
     CDS             90..1016
                     /locus_tag="ACUQR0_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            1255..1314
                     /locus_tag="ACUQR0_003243"
     CDS             1255..1314
                     /locus_tag="ACUQR0_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HHV9110169.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1631..1690
                     /gene="ibsE"
                     /locus_tag="ACUQR0_003244"
     CDS             1631..1690
                     /gene="ibsE"
                     /locus_tag="ACUQR0_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HHV9110170.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1810..3243)
                     /gene="hldE"
                     /locus_tag="ACUQR0_003245"
     CDS             complement(1810..3243)
                     /gene="hldE"
                     /locus_tag="ACUQR0_003245"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HHV9110171.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3291..6131)
                     /gene="glnE"
                     /locus_tag="ACUQR0_003246"
     CDS             complement(3291..6131)
                     /gene="glnE"
                     /locus_tag="ACUQR0_003246"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HHV9110172.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6154..7455)
                     /gene="ygiF"
                     /locus_tag="ACUQR0_003247"
     CDS             complement(6154..7455)
                     /gene="ygiF"
                     /locus_tag="ACUQR0_003247"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HHV9110173.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7697..8317
                     /locus_tag="ACUQR0_003248"
     CDS             7697..8317
                     /locus_tag="ACUQR0_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HHV9110174.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8381..9619
                     /gene="cca"
                     /locus_tag="ACUQR0_003249"
     CDS             8381..9619
                     /gene="cca"
                     /locus_tag="ACUQR0_003249"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HHV9110175.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9782..10603)
                     /gene="bacA"
                     /locus_tag="ACUQR0_003250"
     CDS             complement(9782..10603)
                     /gene="bacA"
                     /locus_tag="ACUQR0_003250"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HHV9110176.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10694..11062)
                     /gene="folB"
                     /locus_tag="ACUQR0_003251"
     CDS             complement(10694..11062)
                     /gene="folB"
                     /locus_tag="ACUQR0_003251"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HHV9110177.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11167..11784
                     /gene="plsY"
                     /locus_tag="ACUQR0_003252"
     CDS             11167..11784
                     /gene="plsY"
                     /locus_tag="ACUQR0_003252"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HHV9110178.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11797..12728)
                     /gene="ttdR"
                     /locus_tag="ACUQR0_003253"
                     /pseudo
     CDS             complement(11797..12728)
                     /gene="ttdR"
                     /locus_tag="ACUQR0_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
BASE COUNT         3130 a         3641 c         3230 g         2962 t
ORIGIN      
        1 tgtcgttctc aaaatcggtg gagttgcatg acaaggtcat cgggcattat ctgaacataa
       61 aacactatca gtaagttgga gtcattaccg aacaacaaga agcgtttatg acgcttgagc
      121 aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg gcttttgaag
      181 ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa attcaggaaa
      241 cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa cgtgaagttc
      301 gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg aaatcgatcg
      361 ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac cttgcactcg
      421 cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca gaagccgatc
      481 gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa gcggttgaac
      541 tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg gctatcgttg
      601 agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa cgtgcgctga
      661 acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa ctggccttgt
      721 tgcaggcgct acctgcggta atagaaaaat ccgttgagcc gatgaaatct attgacggta
      781 tcaagattat tcaggtcgat ggtctgaata gtggcagcgc tgcgggtgat gcaaacacgg
      841 gtaatgtgga gggcggaaac ctagcggaac aagcattatc agccgctctc tcttaccgca
      901 cacaggctcc gctgattgac tccttgctca atgaaattgg cgtttcaggc ggttcactgg
      961 cggcattgac ttcaccctta atctcaacaa ctcccgtcgc cgaaaacgta aaataaattt
     1021 tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc gttccgctaa
     1081 aaactgcaaa tgatcaggtc gttgcaacgt tttgcgtgac cgctcgggga aggcaaaact
     1141 gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga gtcagcaaag
     1201 ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag ggttatgatg
     1261 aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta ttaacagcca
     1321 atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt gaaaaaaaat
     1381 cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg gattttttat
     1441 atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg cgtgaccgct
     1501 cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct catcaacaat
     1561 cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg ccttatagaa
     1621 ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt taagtttccc
     1681 gacttactaa caactgatca gaggggggag aaatcctccc ttacccttgt tcctttactt
     1741 taggttgaaa aaacaacagc gtcaataggc ctgcctgcta cgaagcgaga tctgtgaacc
     1801 gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag tggtcgagca
     1861 accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt ctttactccc
     1921 ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca ggatcccggc
     1981 gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg cttccagtgc
     2041 gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgagaatccc ctttcagccg
     2101 tttggtggag gcatcgctgt tcacagcgac aatcaagcgg tcacccagtt tgcgggcatt
     2161 tgccagatag aagacgtgtc cggcgtgcag gatgtcaaag acgccattgg tcatcaccac
     2221 tttttcaccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt cggtcatcac
     2281 gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg gcgaaaccgt
     2341 ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga agcaggcttc
     2401 ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg tgtcgcccgc
     2461 accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg ctttacccgg
     2521 ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga gttcgtaatc
     2581 ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt taccgacaac
     2641 agcttcaaat tccgagagat tcggtgtcag cagcgtagcg ccgcggtagc gctcaaaatc
     2701 ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt ggatcatctg
     2761 ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc caatcgaact
     2821 cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acaccttcga aaccttcttc
     2881 aaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg tgatggtcgg
     2941 atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag atttactcag
     3001 cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag caccgagaga
     3061 agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg tattcacttt
     3121 aaccacgggc accggcgctt ccggcgagat acgactggtg gggccgtacc agtaacgatc
     3181 cagcatcaca tcaccaacca ccatcactcc tgcacgttca aactctggca gcgttacttt
     3241 cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact tcattcttcc
     3301 accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa gcaatcctcc
     3361 gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg cagcgtcgtg
     3421 taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg cgccagcagc
     3481 tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg agcgtagcgc
     3541 aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc ggctttgata
     3601 tcaaagcgat tacgatgttt attgccgaga tgagcgcgca ttttctcgcg catttcccgc
     3661 acttccgttt gcagagtttt accttctcgc ggcagtgtca taatctcacg acgcaccgcg
     3721 tcaaagtgcg cagtgagctg cggatcgccg tacactacac gcgcacgcac cagcgcctga
     3781 tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgc ggatgtcacc
     3841 agcattcccg ccgccccgga cggacgcagt cgagcatcca cttcatacaa aatgccggaa
     3901 gaggtacgcg tactgaacag atgcataatg cgttgcgcca gacgcagata aaactgccgc
     3961 ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg gaggaagata
     4021 aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta gccgaccacc
     4081 gcaaaaccgc gcccttcacg ttcgttgagg tgatttggct taccgtagcg ggccaccatt
     4141 tgaccccacg cctgctgaac gacggcatct atcatagctt ctgccagcca ggttaagtga
     4201 tcgctcactt tcattaccgg tagcgtaccg gcgatatccg ctgccgcaat gcgtaacagc
     4261 tgcgcctgtt tgaactgacg cagcgcctca agctgttgtt cttcgtcatc ttccggcacg
     4321 cgcagcaaat actggcgcag ttcatcacgg taggcatcgg tcgccgtcgg ctgataaagg
     4381 gtatttggat cgagcaattc atccagcaat aatggataac gcgctagctg gctggcaatc
     4441 atcggcgatg cggcacacag agaaatcaaa tgtttaagcg ccgcagggaa ttcactgagc
     4501 aattctaaat aggttgtgcg ggtaacaatc cccaccagca aggcggtaat gcgcgacagc
     4561 gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgtggcat cagatggtcg
     4621 agcacctgac gtcctcgcgg cccgatagta cgcttatcca gctctttgcg gaaatcagca
     4681 atcagcgtta ggacctgttt gcgatcatcc tcgctaagat gcgccagcac tggcgtagtg
     4741 tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag cgactcttcc
     4801 tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac attggtcata
     4861 tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc ccacgccagc
     4921 cgcgcacggt taagctcatc agaaggaagc gtttgggttt gttcgtcgtt aatgctttgc
     4981 agcaggtttt ccagacgccg caggaacaga tatgccactc gcaattgttc agcatcgttt
     5041 tcagaaagca gatgcagcgc ggcaatggcg ctgagcgttg gcagtaaaga gcgcgactgc
     5101 agcaacggtt cgcgcccacc gcgaatgagc tggaacacct gaacgataaa ttcaatttcg
     5161 cgaatgccgc ctgcgccgag tttgatattg tcggtcaaac cacgtcgacg cacttcacgg
     5221 gcaatcatcc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat gtaacgacgg
     5281 aaaacaaacg ggcgcagcat cgcacgcaac tcgttagcat agacgccttc gctatcgccc
     5341 ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gtccctgctc ctggtaataa
     5401 tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg acgcagtcgc
     5461 atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt aatcagccgc
     5521 tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc ctgcgtacaa
     5581 ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag ttccccgccg
     5641 cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc attgcacggc
     5701 gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac aatcagcgtc
     5761 tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc cagcgtttgc
     5821 gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg cattaacccg
     5881 gcttcgtcgc tcacattact gagcgcctcc tgcaaccaca cgctgtaatg ttgccattcg
     5941 tcagcctgtg gcggttggct ttccagttcc gtcagccact ctggatgcgc aatcacgctg
     6001 tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcactaag tgattcctcg
     6061 gctaaaagct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa cggtgaagag
     6121 agcggcttca taagcgattt tatccttgcc tgattaacgt tttccactgt gcaaccagaa
     6181 cggttcctga ttgtttgcct cattacggaa atgttcaatt tcaatgcgtt gcccggtcgc
     6241 aatagcgtga cgcagcccct gccagttctc cagccaggct tgcgcgacga caggatcata
     6301 gtaacccgcc agcaacagta ttgagtcaat atcacgcatc aggcgtggca actggtcacg
     6361 gtagcgatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat ggcgggaaag
     6421 atggatatcg gcaaagcgtt tgaaggagtc gctaattttg ccctgcgctt tggcatctaa
     6481 aaacggctgc catgctttgc ttaccagcca ttcggtcaac gccagcttcg ccattgccgt
     6541 ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcgatggtcg cttcgcattg
     6601 agtcagcaaa tcacgtaagt gagtactcgc tttacgcggc acaatgccac cgaacagcat
     6661 cagtgtatga cggactaggc taatggctgc cagcacctgc tctttcgccg catcgttgcc
     6721 gcgtacccac agttcttcat gatactgcca ttgcgctaac accagctcga gcgccgcttc
     6781 cagcccctgt tccacatcgg cttttgccgc aacatgcaaa atggtggtcg gtttgatttc
     6841 acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca ggctgccctg
     6901 gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc gcgtgtcgcc
     6961 gctaagcagc tccagttcca gctcgcagat aggctcagca aattcacccg ctttcacttc
     7021 cccctggtcg agagcgattt caattcggct gccattgatc tctactagcc atttttcgcg
     7081 ataaaaatcg gtgctaaaca gcgactgcac gcgggaagcg agatcggcag gcaattcgcc
     7141 gttcggccag atttccgtcg gtaactgcgc caggtcgagc gtcggttcgc tcaacgccac
     7201 gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt tcatggtcat
     7261 ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac gcagccagtt
     7321 atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct cgccgcccag
     7381 cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa actttaattc
     7441 gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg atgaacggcg
     7501 aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc catgcaacgg
     7561 gcaatttgac gggcgtaaaa gtttgaagca gtggcaaggc ctgacacaga ggattccgat
     7621 tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca ctttccatga
     7681 caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac tcgcacttag
     7741 cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac tgaatacctg
     7801 ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg ccggcgagga
     7861 agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag acagctctgg
     7921 ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc gctcccgtgt
     7981 gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata tcgataacac
     8041 ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca gcgtgatcaa
     8101 cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc agaaaaaggt
     8161 cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc aatggtttat
     8221 gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc cgcacctgat
     8281 cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg ttgcactgtc
     8341 ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt atctggtcgg
     8401 tggtgctgtt cgggatgcat tgttaggact atcggtcaaa gacagagatt gggtggtggt
     8461 cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc gcgattttcc
     8521 tgtgtttctg catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg aacggaaatc
     8581 cggttccggt tacaccggtt ttacttgcta tgccgcaccg gatgtcacgc tggaagatga
     8641 tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg gcgagattat
     8701 cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg tttcccccgc
     8761 ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc gttatgccca
     8821 cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga cccatgcggg
     8881 tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg ccctcaccac
     8941 ccgcaatccc caggtgttct tccaggtact gcgcgattgc ggcgcacttc gcgttttatt
     9001 cccggaaatt gacgcactgt ttggcgttcc ggcacctgcc aggtggcatc cggaaatcga
     9061 cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc cgcaggttga
     9121 tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc cagaactttg
     9181 gccgcgtcat catgggcatg gcccggcggg tgttaagtta gtggaacaac tatgccagcg
     9241 tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt ttcacgatct
     9301 catccgcacc ttcccaatgc tgaacccaaa aaccatcgtc aaattatttg attccatcga
     9361 tgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg ctgacgtgcg
     9421 cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc gcgaaggctg
     9481 ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta aaggcgtgga
     9541 gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagctgga aggaacaacg
     9601 ttgcccaaag cctgactgag tataaaacta aacgttagca cgaaccgtcc cctcgcccct
     9661 tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa tcacccccac
     9721 cccggccctc tccctacaga gcgagggaga aaacccggcc tgctcccgaa ccttttctca
     9781 atcaaaagaa cacgacatac accgcagccg ccacaataaa gcgataaatg gcgaacggga
     9841 taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc gccaccacaa
     9901 aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg aagccccagc
     9961 ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc aggaacgaga
    10021 actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg gtcgccccgg
    10081 aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata aacgcctgac
    10141 gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg cattcggcgg
    10201 caatcagcaa caaaccgcca acgaccagcg catacatcac atttatcggg ttaaacaatg
    10261 acttaatcgt gtcgtggaat aatagcccca gtaccaccgc cggaatcatc cccagcaaaa
    10321 tgtggatcag cgttaaacga cctttgcttt caccttcgtg ctgcaacggg cggccaaagt
    10381 ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc agaattgatc
    10441 ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcaaacccc aacaagtgac
    10501 cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat ccttcgacca
    10561 cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa cccctaaatt
    10621 attaaaatgt accgcttgtc cgaactactg cagtatgacc aggttataac cgtttggttt
    10681 aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg attacgccaa
    10741 cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatacgcacc cacggcgagt
    10801 tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc agcgcaaaac
    10861 gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg aggcaatccg
    10921 ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata tcgaacacta
    10981 acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt accgaaagtt
    11041 gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg gataccactt
    11101 ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt caaaacggaa
    11161 cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg cggctccatt
    11221 tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac cagcggctcc
    11281 ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc cgtagcagta
    11341 ctgattttcg acgttctgaa aggaatgttg cctgtctggg gcgcgtatga attaggtgtc
    11401 agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat ctggcccgtt
    11461 ttcttcggtt ttaaaggagg aaaaggcgtt gctaccgctt ttggagcaat cgcaccaatt
    11521 agctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct attgagcgga
    11581 tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt ctggtggttt
    11641 aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct gcgtcatcat
    11701 gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa attcaaaaga
    11761 aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat agccttcatc
    11821 gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccgcgcaga cacgaatttc
    11881 atgctgcgat agagcagctc ccggtaaaca gcccagatat tggcgctttg tgcatactcc
    11941 ggcaatactt gcaccagttt gccactctcc agaaacggca acacatccca ctcggaacgc
    12001 agcataatcc ctttgccctc cagcgcccat tgcaacacta tctcgccgct attggaggaa
    12061 agatgcccgc taaccttcac cgattttttc tcctgaccgt tccccaactc ccatattccg
    12121 tgggtcatat cgcgctcttt ggtcaccaga cagtcatgac gacttaattc ctgtaatgat
    12181 tgtggctgcg gatatttttg cagatactca ggtgctgcgc ataatattct tttatttttc
    12241 gttaacagat gcgcaatata ataatcagga atttcgtcat taatacgaat atccagatca
    12301 atattatcct gcactaaatc aatttgccga tcgaacaatt caaaatgcac ctgtaactca
    12361 ggataattgc gcataagttc ggtaatggct ggcgcaatat ggctgcgccc aaaaccaaaa
    12421 ctgcaaccaa tacgaatcat cccctccggg cgcgttttga tttgcgtgac gtcatcgacc
    12481 agtcgctgat attgggtgag gatttccagc gcatgttcgt aacaacgttg gccactttca
    12541 gtcagcgcca cgccgcgagc ggagcggttc agcagcgttg ttgccagcgt attttcaagg
    12601 atttggatcc gcttggtgac aaatgcaggc gtttgcccaa gcgtcgccgc tgcagcacta
    12661 aaactcccgc tgtggacgat ttctaccagt acctgcaagt ctttggctaa aggccagcta
    12721 ttcagcatcg ttaattatcc gcagttgtga taagcgcagt gtatttcgca aaacattgcc
    12781 attccctgct ctggcgcaat attcattctt attttcgcgt tgattcacga actgttaatt
    12841 acatattcac ggtggcaaaa aatataaaac cacattttta gtgttagttt gtggcggtga
    12901 atttttccag acaaatacaa aaactggagt tgccatgatg agcgaaagta ataagcaaca
    12961 ggc
//
LOCUS       DBKELA010000106        12883 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.106, whole genome shotgun sequence.
ACCESSION   DBKELA010000106 DBKELA010000000
VERSION     DBKELA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.106"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACUQR0_003254"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACUQR0_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HHV9110179.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACUQR0_003255"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACUQR0_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HHV9110180.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACUQR0_003256"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACUQR0_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HHV9110181.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..3249
                     /locus_tag="ACUQR0_003257"
     CDS             1993..3249
                     /locus_tag="ACUQR0_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HHV9110182.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3542..4783
                     /gene="agp"
                     /locus_tag="ACUQR0_003258"
     CDS             3542..4783
                     /gene="agp"
                     /locus_tag="ACUQR0_003258"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HHV9110183.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4821..5048)
                     /locus_tag="ACUQR0_003259"
     CDS             complement(4821..5048)
                     /locus_tag="ACUQR0_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HHV9110184.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="ACUQR0_003260"
     CDS             complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="ACUQR0_003260"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HHV9110185.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6038..6145
                     /locus_tag="ACUQR0_003261"
     CDS             6038..6145
                     /locus_tag="ACUQR0_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HHV9110186.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="ACUQR0_003262"
     CDS             complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="ACUQR0_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HHV9110187.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="ACUQR0_003263"
     CDS             complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="ACUQR0_003263"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HHV9110188.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(8081..8671)
                     /locus_tag="ACUQR0_003264"
     CDS             complement(8081..8671)
                     /locus_tag="ACUQR0_003264"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HHV9110189.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="ACUQR0_003265"
     CDS             complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="ACUQR0_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HHV9110190.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="ACUQR0_003266"
     CDS             complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="ACUQR0_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HHV9110191.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="ACUQR0_003267"
     CDS             complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="ACUQR0_003267"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HHV9110192.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="ACUQR0_003268"
     CDS             complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="ACUQR0_003268"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HHV9110193.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11958..12596
                     /gene="rutR"
                     /locus_tag="ACUQR0_003269"
     CDS             11958..12596
                     /gene="rutR"
                     /locus_tag="ACUQR0_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HHV9110194.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(12636..12701)
                     /locus_tag="ACUQR0_003270"
                     /pseudo
     CDS             complement(12636..12701)
                     /locus_tag="ACUQR0_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3279 a         3243 c         3278 g         3083 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgactg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccgcgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagcctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag
     2461 tattgtttag atgatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata
     2521 tctgatttag atactggtat tgatttgtct ggcatatctt ttattcagag tgaaattaat
     2581 aaaacatatg gattaaaata cgctccagta gatggcgatg gatattgtct gttaagagcc
     2641 atactggttt taaaagaaca tgagtattca tgggcgctgg gcagccataa gacgcaaaag
     2701 caagtatacg aagagtttat taaaattgtt gataaacaaa cgatcgaggc tcttgttgat
     2761 acggcattct ataatctcag ggaagatgta aagacattat ttggcgttga tctgcaatct
     2821 gacaacaaaa ttcagggtgt gggtagtttt atgtcatgga gctttctgtt ttttaaaaaa
     2881 caattcattg atagttgctt gaatgacaaa aaatgcatac tgcatttacc cgagtttata
     2941 tttaatgata acaagaattt gcttgcttta gataccgaca cgtcggatag gattaaagcg
     3001 gtgaagaatt ttcttgctgt tctttcagat agcatttgct cattatttat tgttaatagt
     3061 aatgtggcat caatctcctt gggaaatgaa tccttttcaa cagatgaaga tcttgagtat
     3121 ggttatttaa caaacactgg caatcattat gatgtttacc tccctcctga acttttttct
     3181 caggcttata agttaaaaaa taaggaaatg aatgcgcaac ttgactattt aaatcgttat
     3241 gcaacttaat ggcaaaggca tatgctaaaa accattgtta ttagtctcac acttttttat
     3301 tggtaaatat tgtctctgta ttggtaacgc cgcagatatt ctgtttagcc acaggtgcaa
     3361 ttatcagcgg cgtacgcgag gcaggggcta atcaggcata gtttgcgtca aaccttgcct
     3421 gtttttgaag atgtatatag aaaaacaggc gttcaacaag ccattttgcg aacctgttcc
     3481 cggaaaaaag tcatatttct gtcacactct ttaatgattg ataacaaaag aggtgccagg
     3541 aatgaacaaa acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc
     3601 tcaggcacaa accgtaccgg aaggctatca gctacagcaa gtgctcatga tgagccgcca
     3661 taacttacgt gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga cgccgaataa
     3721 atggcccgaa tgggacgtcc ccggtgggca actcaccacc aaaggtggcg tgctcgaagt
     3781 gtatatgggc cattacatgc gtgaatggct ggcagagcag gggatagtga aatcggggga
     3841 atgcccgccg ccggacaccg tttatgccta tgccaatagt ctgcaacgta ccgtcgctac
     3901 cgcccagttc tttattactg gtgcattccc tgggtgtgat attcctgtgc atcaccagga
     3961 aaaaatgggc actatggacc caacctttaa cccgatgatc accgatgatt ccgccgcatt
     4021 cagtgaacag gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag
     4081 ctaccagcta ctggaaaaaa tcgttaacta taaagattcc cctgcctgta aagagaaaca
     4141 acagtgttcg ctggtggatg gcaaaaatac ctttagcgcc aagtatcaac aagaaccagg
     4201 tgtttccggg ccgctgaaag tcggcaactc gctggtagat gcgtttactt tgcaatacta
     4261 cgaaggtttt ccgatggatc aggtggcctg gggagaaatc aaatctgacc agcagtggaa
     4321 ggtattgtcg aagctgaaaa acggttacca ggacagtctg tttacctcac cggaagtggc
     4381 gcgcaatgtt gcgaaaccgc tggtcagtta tatcgacaaa gccctggtca ccgatcgcgc
     4441 cagtgctccg aaaatcacgg tgttagttgg gcacgactcc aacatagcct ctctgttaac
     4501 ggcgctggat ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg
     4561 caaaatcgtt ttccagcgtt ggcatgacag caaagccaat cgcgatttga tgaaaattga
     4621 atatgtgtac cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc
     4681 tgcgcagcgc gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttctgccc
     4741 gatggataag tttgatagcg tgttgaatga ggcggtgaaa taacagaaaa ctcccccgcg
     4801 agaagcgggg gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa
     4861 agcgaatctt tatcggtctt agcaaaggct ttgactaaca cttcatcact accttcttcc
     4921 caaatctttt ctgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt
     4981 tccggcgacg tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga
     5041 gttggcatgc gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga
     5101 catattcccc ttgataacga gcaatagaca gttcttcctg gcttggctgg cgtgagccgt
     5161 caccgcctgc gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa
     5221 ataattcctg cgctgcgtag ccaatgggga caatcaccat gccgtgatgc gcaagggtcg
     5281 tccaggtgga tgtgatggtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc
     5341 tcgccagttt tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg
     5401 tacgcatttg accggacatg ttgccaaagc gggtaggtgt accaaaaata atggcgtcgt
     5461 aatcggccag ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt
     5521 caaataattg cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcca
     5581 ctttgcttgc accctcagcg actgcgcgtg ccatcgtttc aatatgtccg tacatggaat
     5641 aataaagcac cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag
     5701 cgattcgcta ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc
     5761 acttatttga tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt
     5821 ttatctcatc gcgttttttt aatcataaga gcggcttatg gataattatt ggagatgata
     5881 tctattctcg ctaagaagct gttgcaggat attaccaaac gcgggtctgt ccgcgtcagt
     5941 tcactaagct tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct
     6001 atgcaatatg atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc
     6061 cggcaatttt gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggcaagag
     6121 cagtcacggc aaaagcgaca actagccggg ctaatcaatg acgaatgcat ttttttctta
     6181 agctcgtcaa aaagtcatca ccgccggtaa cccggcggtt ttctgtttat ggctcctgat
     6241 gaacaacttc tggcggtgga acgtcaacca accttctgct taataacgca ttgagtaaaa
     6301 tcgcgccaaa ggttgctgta ccgatccctc ccaacgtaaa accgccaagc gtgagagcaa
     6361 aatcacccgc gcccagcacg aaggttactg cgaccataat caaattagcg ttctggctta
     6421 aatcgacacg gttttgtacc catatccttg cgcctgcgac ggcaatcagc ccgaacacaa
     6481 caattgatgc accaccaatc accgcggccg gaatggtatg gatcagcgcg ccaaatttcg
     6541 gcgaaaagcc caacagcata gcgatgacgg cagctgcaac aaacaccagc gtcgagtaga
     6601 cttttgtcac ggccatcaca ccgatatttt cagcataggt ggtgacgccg ctaccgccga
     6661 cagagccgga aagcatcgtt gccagaccat caccgacgaa tgctcgcccc atatacgggt
     6721 ccatattgcg tccggtcatc ccggcgaccg ccttgagatg acctaagttt tccgccacca
     6781 gaatcaccgc cacgggcgca atcaacatca tcgcttggcc attaaaacca ggagtggaaa
     6841 aatgcggcag accgaaccag gcagcatagc tgacgagagt aaaatcgacg gcttttccca
     6901 gccctaaaac gttggtcatc acgccataca gcagacaggc gacaattaat cctacgagaa
     6961 tcaataaccg ctggatcatg ccacgggtaa acaccgccac cagcccaata cacagcaccg
     7021 tcattaccgc catccagcta tcaaaggccg aagccgatac acttttcact gcgataggcg
     7081 ctaagttcag gccaatcgcc atcaccaccg cacccgtcac caccggcggc attagtcgtt
     7141 caatccagcg tgtaccaatt ttcatcacca ccaggccaat gacggtataa accagcccac
     7201 aggcgataat cccgcccagc gcgatgctga tattcgggtt aatgccctga ccgttaaagc
     7261 ccgtcgcggc aatcaccacg ccgacaaaag ccgcgctgga gccgagataa ctggggacgc
     7321 gcccgccggt aataaagaaa aacagtaacg tgccgatccc cgacattaaa atggaaagat
     7381 tgggatccag ccccatcaga atcggcatta acaccgtcgc gccaaacatc gccaccgcgt
     7441 gttgaacgcc cattactgcc gtctgagcaa acggcaatcg ttcatccggc gcgaccacgc
     7501 cgctctctgt agaggtcgat tttaactgcc agtgaggaaa accgaacatt gccatcagct
     7561 gtctccttaa ggaggttaac aagcagggcg catcagcgcg tgataactgc gatcgaacca
     7621 caccagcccg tagggtgtgg tgtgacgatg aatcgcttcg atggcgcaaa acagaatgtc
     7681 gtgggtgccg acgctcacca cctggctgat acggcagtca aacgaaacca gagcctcttc
     7741 cagttgcggg catccggtca cacccgtctg ccagcgggcg gcggcaaagc ggtgttccat
     7801 gggcgttttg ccgccaaaaa gatttgaaag cggctcctgc ccggcgctca gtgtatttac
     7861 acacagcgtt cgattttcat tgaataccgg ccagacggac gccccacgat tcaggcacac
     7921 cagtaatgtg ggcggcgtgt cggtcacgct gcagacggcg ctggcggtga acccggcgcg
     7981 cccggctgga ccgtccgtgg tgatgatatt gaccgccgcg cccatacagg acatcgcatc
     8041 gcgaaaggtt tgttgatcaa ctatgttcat agtttgctcc ttacaacagc ccgcaggctt
     8101 cttcaaagga cagacgtggc aggcgcgcaa aaagtttgct gctatcgcca tagccgatat
     8161 taatcagcag attgctcttc agcgtgctgc ccgcaaaaaa ggcgtcgtca acgtgttgac
     8221 ggtcaaaacc cgacatcggg ccggtatcca gtcccagcgc acggcaagcg acgatcagat
     8281 aagccgcctg catggaactg ttgcgaaacg ctgtttcttc ggcaagttgt gggctggagg
     8341 taaaccaact gcgggcatca ccgtggggaa acagtagtgg taaccgttca taaaattcac
     8401 tgtcccaggc gacgatagcg gtgacgggcg cggtcagggt tttttgcaga ttgccgctgg
     8461 aaagcgccgg gcgcagacgt tcttttcctt ctgcagtgcg gataaacacg atccgtgccg
     8521 gagaacagtt agctgatgtc ggcccccatt tcatcagggc ataaagctcc tgtaacgtct
     8581 catcgctgac gggtgtctcc cgccagccgt tgtgagtgcg ggcatcggtg aacagggtgc
     8641 taagcgcacc tgggctaacg gcttcgttca tagcaattcc ttacagggcg gcttcacggt
     8701 gatgtaacag gctggcaagc ccgttgagta acagagcatt aaacgtttcg ggatcggtca
     8761 cgttgcaggc gtgtccgcca tagggcatca ccattttctg gctatcgggc agggcggcat
     8821 gaagttcact ggaacatgct gttggcacca gcagatcatc actggcgcag atgatttgca
     8881 ccgggcagcg gatgcgatcc gcatggtgac taaagtcagc gcgtttgagg gcgttaagtc
     8941 gacgtagtaa attatttttg ccctgaaaat gcgccagtgc cagcgcgtct tctgcctcca
     9001 ggcgaggtgc gcgggccgcc atccagtcgg cgggatagag gaacaacggc tgcgcttcca
     9061 cccatgcttg cgcgccgccg ctatacagta atcgttcgcg aacctgaaaa cagcggcgcg
     9121 tatgggcgtt tattcgtagc cagccgttaa cgctgaccag catagttacc gacgcgggat
     9181 aatccagcgc cagctgcatc cccaccagcg caccgagcgc atggccgacc accgcgtaac
     9241 gctcaatccc tgcggctacc agcgcctgat gcagttccgc tgccatctgg gcgatactgt
     9301 aatcttctgc cagtgtgtcg ggattattgc cggtgccgcg ctggtcgtaa cagactacct
     9361 gatactcctg ctccagcacc gccagttgcg gtaaccagta actgccgcta ccgccaagac
     9421 ccgaaatcaa caccactacg ggtgcatcag cataaggggg aggtgagagt gaaagtttca
     9481 tcgcggcctc acttggcgat atgcgcaatt gtggcgattt ccaccagcgc gtcaggtttt
     9541 accagtccgc actgaatgta gaatcgcgcc ggtttatcac ccggaaaaaa ctcggcgtag
     9601 atttcgttaa tcgcggcgta atttttccag tcggtaataa agatgctgtt gaaggtcaca
     9661 tccgccatcg tgccacccgc cgtctcgatc accttgcgga tagtttccag aacgtggcgg
     9721 gtttgcgcct ttgggtcatc ggcaaacagc acgttattat gttgatcaaa agccagcgta
     9781 ccggagacat acaccacgcc atcagccagc gtgccgggaa cgaagggggc cagcggtgcg
     9841 ctgctgccag cgggaataat tacggatttt ggcatcgcta aactccttaa gcgatacgag
     9901 caaaggacgt gggagaaagc gcgtcgcaga acgtttcgac gtcgctgacc cagccaaaaa
     9961 aggtttcgat attgaacaac gcggctttct gtgcaaattc cggccccgcc tggtgtgttg
    10021 cgtcttccag caccacgccg aaatgctcca gaaaaaagcc atcgcgtagc gtcgattcga
    10081 cgcagacgtt ggtagcgata ccggtgaaaa ccagatggcg tattccacgg ctgcgtaaaa
    10141 tgctgtccag cggcgtattg aagaaaccgc tgtagcgcgg cttcggcagc acaatatcgc
    10201 caggctgcgg caccagttca tccaccagtt gataatccca ggagcctttc gccagcaatt
    10261 tcccctgcag ctgcggctgc ttacgcatgg ttttcagggc gttcgattta tgaaaattcg
    10321 gtgagccggg gccgccagcc tcgacatact gttcatccca gccattttga aaccagatga
    10381 tcaacatccc tgccgctcgc gctgcggtca cagcggtttg aatgttggca atgaccgggc
    10441 gagtggttga gacatcaaac ccggcgagat ctaagtagcc gcctggcgtg gcataagcgt
    10501 tttgcatatc caccacgatc tgcgcacttt gctgcggatc gaaggtaatg gcttccggtc
    10561 gagcggttaa ggttgtcatc atgccacctc ctgagtcagc gcagagagat gggcgcggca
    10621 ctgcatcagt ggttgaatgc gctcgccgaa ggtttcgatt cccgacagaa aatcatcgaa
    10681 ggtcaacagc acgccttctg caccaggcac gcttgcgact tcatctaaca tgcgcgcgac
    10741 actggcgtaa gaaccgacta acgtccccat attgatgttt accgccgaag tgggatcggc
    10801 catctgacga acgttggtgt cagtaccgga gcgggtatct ttctgacttt gttcggttag
    10861 ccagcttaac gcctcttcat ccgcgcccgc tttgtagtgt tcccatttgg cgcgagtggc
    10921 atcgtcggtt tcatcggcaa tcaccataaa caacacataa gagccaacgt cgcgtccggt
    10981 ttgctctgtg gcctgtttca tccgcgcagc ggtcggggcg aaagccgtgg gtgtatttac
    11041 gcctttgccg aaacagaagt tgaaatcggc atactgggcg gagaacgcca tgccagcgtc
    11101 gctttgcccg gcgcagatca ctttcatggg gatactcggt tgcggactga cgcgacaatc
    11161 attcatggtg aaaaaatcgc ctttaaaatc gcttttcccc gtgccccaca ggtcgcgcag
    11221 cacctgaaca tattcggtga gatagtcgta acgacgggag aaatagtcat cgccaggcca
    11281 gatacccatc tgctcatact cgggcttttg ccagccagtc acgaggttga cgccaaaacg
    11341 cccgccagag atggagtcga tggttgcggc catacgggcg acgattgccg gaggtaacgt
    11401 taaggtggca gcagtggcgt aaatctgaat gcgcgaggtc acggctgcca gacccgccat
    11461 caaggtgaac gactcaaggt tgtgatccca gaactcagtt ttgccgccaa agccacgcag
    11521 tttgatcatc gacagggcga aatcgaaatg gtaatgctcc gctttttgcg caatggcttt
    11581 attcagttca aaggtcggca tgtactgcgg cgcgtgggtc gaaatgagcc agccgttgtt
    11641 gccaataggt acgaatacgc caattttcat catcaacctc tcttcgtctc gtaaagtgaa
    11701 agtcagacgg ggcgctgcat cctgcatatc cttttcagcc gcgtattggc ttgtttgcaa
    11761 agcggatgcc agtttttaaa aagtgaatgt tattaatgtg ttagcattgt gttatctaaa
    11821 atatctggta aaaagtggac taaacggtca aaacagttgc acataaaaca tgcatccgtg
    11881 cgcgatgaga gtgcagaagg tcgaggccgg gcgggggttt tgctatcctg ttaccaatct
    11941 acaagagggg agagcgcatg acgcaaggcg cagtgaaaac aacgggtaaa cgttcgcgcg
    12001 cagtaagcgc gaagaaaaaa gcgattctta gcgcagcact ggacactttt tcacaattcg
    12061 gttttcacgg cacaagactg gagcagaccg cagagttggc gggtgtttca aaaaccaatc
    12121 tgctgtatta cttcccgtca aaagaggcgc tgtatattgc cgtgctgcgg cagattctcg
    12181 atatctggct ggcaccgtta aaagcgtttc gtgaagattt cgccccgctg gcggcgataa
    12241 aagagtacat ccgtctgaag ctggaagtct cacgcgatta tccgcaggct tcgcgcctgt
    12301 tctgtatgga gatgctggca ggcgcgccgt tgttaatgga tgaactgacg ggcgatttga
    12361 aggcattaat tgatgagaaa tcggcgctga ttgccggttg ggtcaaaagc ggcaaactcg
    12421 cgccgattga tccgcagcat ttgattttta tgatttgggc ttccactcaa cattacgccg
    12481 atttcgcccc tcaggtggag gcggtgacag gcgcgacgtt gcgcgatgag gtatttttca
    12541 atcaaacggt tgaaaacgtg cagcggatta ttattgaggg gattcgacca cgttaaagat
    12601 gacggaggag gttgtaacat cctccggcta cctgtttaac cgatcgtcat taaactggcg
    12661 ttaccgccag cggcagcggt attcacactc agcgaacgtt catcgatagg aattaaaacc
    12721 ccaaaaagat taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctccat
    12781 atgaacggaa ccgacgatta aactggatgg ctctgattga ttcagggtat gaatggcggt
    12841 tttttgctcc gtttccctca aaatggacgc aacttcccct ctg
//
LOCUS       DBKELA010000107        12811 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.107, whole genome shotgun sequence.
ACCESSION   DBKELA010000107 DBKELA010000000
VERSION     DBKELA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.107"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            254..330
                     /locus_tag="ACUQR0_003271"
     tRNA            254..330
                     /locus_tag="ACUQR0_003271"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:288..290,aa:Arg,seq:tct)
     gene            complement(376..1137)
                     /gene="envY"
                     /locus_tag="ACUQR0_003272"
     CDS             complement(376..1137)
                     /gene="envY"
                     /locus_tag="ACUQR0_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HHV9110195.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(1320..2210)
                     /locus_tag="ACUQR0_003273"
     CDS             complement(1320..2210)
                     /locus_tag="ACUQR0_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HHV9110196.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2211..5179)
                     /gene="nfrA"
                     /locus_tag="ACUQR0_003274"
                     /pseudo
     CDS             complement(2211..5179)
                     /gene="nfrA"
                     /locus_tag="ACUQR0_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(5166..7403)
                     /gene="nrfB"
                     /locus_tag="ACUQR0_003275"
                     /pseudo
     CDS             complement(5166..7403)
                     /gene="nrfB"
                     /locus_tag="ACUQR0_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
     gene            complement(7553..8995)
                     /gene="cusS"
                     /locus_tag="ACUQR0_003276"
     CDS             complement(7553..8995)
                     /gene="cusS"
                     /locus_tag="ACUQR0_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HHV9110197.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(8985..9668)
                     /gene="cusR"
                     /locus_tag="ACUQR0_003277"
     CDS             complement(8985..9668)
                     /gene="cusR"
                     /locus_tag="ACUQR0_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HHV9110198.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9825..11207
                     /gene="cusC"
                     /locus_tag="ACUQR0_003278"
                     /pseudo
     CDS             9825..11207
                     /gene="cusC"
                     /locus_tag="ACUQR0_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            11231..11563
                     /gene="cusF"
                     /locus_tag="ACUQR0_003279"
     CDS             11231..11563
                     /gene="cusF"
                     /locus_tag="ACUQR0_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HHV9110199.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            11579..12298
                     /locus_tag="ACUQR0_003280"
                     /pseudo
     CDS             11579..12298
                     /locus_tag="ACUQR0_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            12504..>12811
                     /locus_tag="ACUQR0_003281"
     CDS             12504..>12811
                     /locus_tag="ACUQR0_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HHV9110200.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     "
BASE COUNT         3090 a         3267 c         3329 g         3125 t
ORIGIN      
        1 gttggtttca tagttaccag tctcatagga gcggacaatt ttccgttagg gcggtaaatt
       61 gtactttgat acatgaaaat acgggttttc ttgattcaga cgcgcagcgg tgtgcgtttg
      121 tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg tcgttcactt gttcagcaac
      181 cagatcaaaa gccattgact cagcaagggt tgaccgtata attcacgcga ttacaccgca
      241 ttgcggtatc aacgcgccct tagctcagtt ggatagagca acgaccttct aagtcgtggg
      301 ccgcaggttc gaatcctgca gggcgcgcca tttcttcttc atttatgccc gtcttatccg
      361 tttccgcttt gcccttcacc acatcacttt ttctcgctgt ttggcgagat aattcaacgg
      421 tgtcaggctg taaaacgcct taaaaacaga gataaagtac gacgtgctgc tatagccaca
      481 taattgcgcc acctgagtga tatttttgtt atccatcaat aacatctgta cggcgtaacg
      541 catacgacac tctgtgacaa tctggctata gctggtattt tcgtttttta atttcttttt
      601 gagcaggctg gggcttaaac atagcgaact ggcgacaatt cgcagattcc agtaatgctg
      661 aatatcgctt tgaataatgc ggcagacgct gtcgcggacg ctgctgcgta agatatacat
      721 cagtagggca ataaaccgcg attgctcaag aaagttagac aatacggtaa aaagtaatgc
      781 gcgcgtcaac tccgcttcgc agggtttgct ggattgctgc ttgctgtggt tggtggctag
      841 ccggaacact tccggtgtac ggctatgaca agcgataacc ggggttgcca gccgcgacca
      901 gggctttaca caggttaatt tcttatttaa aaacaacaga taatcgttga tgatgttacg
      961 gttgatgtgg gtgattaata cgtgatttaa cgttgagagt tcaatgacgt tattgttgca
     1021 cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta gcatggttat tgacgcttac
     1081 ctctacctct ttttcggtca ggatcaccac gcaaggttca ctgctgctca attgcatttc
     1141 gcactcctca gatatcagaa actccgctca aaggatctat gcttcctgca tgagtgatcg
     1201 gcccgttcgc cgataacgat cttctttctt tagcatgctt tttagcaatt aatcttgatg
     1261 gaattctgac gagagcgaaa gaggtaagcc aggtcgtacc cgacttacct ggaggggatt
     1321 taatactcga gaatgccgtg cgcgacgggc aaatagcgca gagagaaata gagcatgtct
     1381 ttaccgcaag aggaacgttt cgctaacagc gaggcaattt ctgcgtccgg tttcggtttc
     1441 gctgtaaagg ttttgccttt gccggcgaca aacagttcat aaacaatgcc gcttgctggg
     1501 gcggagcttt ggcaatcggc gctggcctgt agataacgtt cacgctgttc agcggtaagt
     1561 ttatccacgc cgctgccatc ctgtacccag acattaactc cggttgcttt aacttgttcc
     1621 agcagttggc gatagccatc gggcgacatg tttccggcga aaaaactact gatataaacc
     1681 ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt ttaaccatgt tagcaaaggt
     1741 tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt ccgcgctgat gtaccagcca
     1801 tccggcgtta tgccaggcgc ggcgctccat aatctggctt gctggagatc ggcagccagc
     1861 aggcgattaa gatagctttc cagcgctgca gacgactgtt tctggtgcat aaaaaattcc
     1921 ggatcggcgt tcagcccgac aatcagcttc aggccagtct gttgcgcagc tgcggcacgc
     1981 ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat cgccgtaacg ggtccattgc
     2041 aaaaccaggg tatcgaagcc ttgaaaacgt aactgactca tcagcccctg ccactgggta
     2101 tcggtaacct gactatctcg gttttgtggt tgccagataa tacctttcat cgcaaaggaa
     2161 aaagggctga ccaaaagcag tgtcagcaat acgaaaatga acttacgcat ttaccagtgc
     2221 actccaatgg tgagaaacgc gttgttgcgc tctccgttac gttgattaat cgccttaaag
     2281 gtatgttgat actcgacgcc gagactgact ttgtgcggcc aggcgtcgta gtgcgtctcg
     2341 ccggtccaaa tattccagcg gaccccgaca ccgccaagct gcgcgccctg agtgccttta
     2401 tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa tagtctgtcc gttagctacc
     2461 ttctgatgcc agctgacgcg ataatctgcc gtccacgcct gaatatcctg gcggatatat
     2521 tgcgccgcat cgaggtacag gttttgggca aaccagcctg aaccgttcgg gtgccattcg
     2581 tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc gcagcatggt atcggatgcg
     2641 ccattttgcc cgttcagcgg caactgctgt tcgacggcga ggaaaaagat ctgatcgcgc
     2701 agcggcttcc agcgcagacc ggtgccggac atcggatttt tcaccggcat catcaccccg
     2761 ttttctccgg tatcggcaaa gacgcggcta taaactgaga gcaggtcgcc ttccagcagc
     2821 atattgcgtc cgatgcggta ctcggcttcc agttgtccgt agctacgata gcttttccca
     2881 ggcgctgcac cgccgacatt attgttagcg gtactcattg cgccggaacg caagccgatg
     2941 gaagaatcga aactgaacgt ccagcggcga ccgacctcct catgcaaacg gcggaaattg
     3001 aagcgttgct gattttgctc tggggtcagt ggggttatgc ctgattatca atgtcatcaa
     3061 tcacgagccg ggcgtagtgc tgcgtcgcag gcatgtcatc cagacgctgg ttcacgtagg
     3121 ccagttgtcg gatcagtgcc ggatcgtccg gaagcccttt atgcgcctgt tcgagcattt
     3181 cccgcgactg tgcgatatca ccgctatccc acaaggcgta accaagcgct gcctgggtgt
     3241 tgctattatt tggttccagt tccagcgcgg cgcgcaaatc actcaccgcg gccggaacat
     3301 tatgacgttg gcgataaatt gtcgcccgcg caacgtaaga gttggcagaa ggggcaatat
     3361 tgattgagcg cgtgagatcg ttcagtgcga gttctggctg agctgggata taacgtcgcg
     3421 catgcagcca ccagtattga gcattgtttc ccagctcacg ttgttctgcc tgttgaagcc
     3481 agcgatcgcg agccgcgcca tttcccgctg cctgagcggt gttggcggcg gcgagcagat
     3541 catcattact catgtcgtga agactgattt tctgccaggc cgccagtgcg gtggcgtagt
     3601 cctcaacctg atacgcctga taggctaccg cacgatgttg ccaggcgctc ggttgtcgtt
     3661 gttcggcctg aagccatgca tacaacgcca caccgggtag cgtgtcccga taacactttg
     3721 ccagacggtt ccaggcggca gcatcgtagg aaggcgacat atcgcccagc aagcgaacta
     3781 ttgccgggca attatctgca atacccggca actgactttg ccactgacgt tgctccgcca
     3841 gcggtaaggg tttcgataaa atcgccacct tcgccggcgt tgctaggtaa ggatgacttt
     3901 ccagcagaga cgccagtcgc gccattaaag tctggctgac acgcgcatcg ccctggaaag
     3961 gatagcgttg cagcaataaa tcggcagctt cgcgtgactg ctcgttctgc atcagttgcc
     4021 aggtcagttg atccaggcgg ggaagatttg tcggttcttg ctgatacagc aatcgtgcca
     4081 gacgcagaga ttcagccttg ttacgggtcg ccacgctgac agcataacgc tcctcaagca
     4141 tttcattggc ggggagggtg gcgagcagtt tttgcgctgc gtcgtactga ccttctttta
     4201 acagcaccgg tagcgtcgcg ccaacaacat actggcggtt atcggcaaac tgcaccgtat
     4261 aattcgccaa tgcctgaacg gggttggcgc tgtatttaga taacagatag agccaacttt
     4321 tctcttgtgc gcccgtggta aacagcggct tattttcaat gagataatgc tggaggcgtg
     4381 ctttttcgcc acgataagcc agcgcggtcg cgtaagtaat atatgactga ggatcagtga
     4441 agatcccctg tgattgcagt gccaggatcc gatcgtccag ctgcccggca agaagcacgt
     4501 caaaccactg acggcgttct gccgcgctta atgtgttctg ctggcgtgct tcattgtata
     4561 gcgtatctgc ctgggatcat tgtttcaggt agattgcccg ttgcagcaga tcggttcgca
     4621 gcgtttttcc ttccggcgat gcagcaaacg tcgcatcgtt cagttgcgct ctggcgacag
     4681 gtaattgtgc cagccgcagg gtattctgcc cgacttcact gcgacaacgc agggtcggcg
     4741 cagcgtcgcc cgctttttgc tgggcaagca gttcttcaac agtcgtaacg cttttcactt
     4801 caactggaat agccgccaga ctgcgctcaa gtcgggcatc tcctgggtga cgtttcagtt
     4861 gatcctcaag caacagccgc gcccggtcat catgaccaaa atgacgatag gcttccgcaa
     4921 ggtataaagt cagcggaata ttatccggca cctgttggtg tatatattca aattcgcgga
     4981 tggcggttgc ttcgtcgtta tttttctgtg ccttcagcgc cttatcgaga cggggataaa
     5041 taacaaaatg gcgataatcg ctcagcccca gctcttctgc gctggtgccg atattgtctg
     5101 cgagtgcgct ggtactcaat aaagacgtca gcagtaaacc agaccatccg atgacgcgat
     5161 taaggttatt ctccttcatt ttcggactcc agttgcgcaa cctgttctgt gtttaaacct
     5221 gctttgagta atagtgattg catcgaaact tgtaattcgc gttgaattgt caggacgcga
     5281 tccaacgttt cctggctgat aacgccttcg gtgaccaaaa acttgccgag cggcagagaa
     5341 ctgcgttcat ggcgcaataa caacacgtta attgctgaac gattaatatg accgagcgtg
     5401 gtcagtattt cggcgaacag gaactgatgc ggcacatatt gccgccagat ttcaccggtc
     5461 tgctgttccg tgagccactg atgctgaacc gcattgtaca acattgcccg cggatcgtga
     5521 ccgcgtcggc gtgcatacca gtggcgtaac cctgtgacaa tttgtccccg cagaacaatg
     5581 acgtaacgca ctttgcgtcc gactttacgc gtcagggccg ccagcgaaac cgggtcaata
     5641 ccatcttcac tgccgacaat taactcatca ttttccagac gcagcggcag tactgcataa
     5701 tgcagcgcta cggaggctgg catttcggca atcagcgagg acgggatctg ccaggcatcg
     5761 atggattccc acgccacgcc gttttgctct gccagcgcct gtgccagctg ctcggcgcta
     5821 atcagcccct gcatcagcat tgaaccgccc aggcgtagac cttcgacgcg attacgcagt
     5881 gctgtatcga gttgttcttc agtgatgacc tgattttcca gcagaatttg acctaacggg
     5941 cgcaacgagc gggtatcgcc agtgacgctg gggaagtcat gcgttgtttt atcccacgcg
     6001 acgcgacgcg gatcgccgtg ctgaagaacc tgtttcagca cgcgccagtt agccataaag
     6061 ttaatcaggt tgccccagaa aagacgcagg acggaaagca ggccctgcgt gaggccgtag
     6121 tagccagtga cgaaaatcac tcgctgcacg atgcggttaa ccatcaagcc aaagtttagc
     6181 cacagcaggg tcattaacca tgcgctgccg ctaaaaatag aaaggaaatg ctaggcatcg
     6241 ggccacaaac tttcatacgc cagcaacagc aaaagctgga tcatcaccag catcgcgagg
     6301 aagctgacaa agttactgat tgcccctttg cggtcgcgcc agagaaagta gttcagcgtc
     6361 aggctggagg tccatttatg ggttttaaag ccctggaaaa caatgccgat gatccagcga
     6421 gatttttgtc gaaccgcagt cgaaaaggta tcggggaaat attcgcgcac gcagatcatg
     6481 tttgacgtcc gtgcgtgctg taaaaattta cgctgctcgc gttctttggc ttcgtccacc
     6541 accggaaaac ggacaaaaat ttccgtcata cctttttctt tcaggcggaa gccaatgtcg
     6601 taatcttcag taagactctg cacgtcgaaa gcaataccgt taccgtcagc taacagtgcg
     6661 gtcacggcgc ggcggctgaa acaggtgccg acgcctgcgc tgggcacttg tccggcgagg
     6721 gcttcacgca ccggaacatc tttgccatgc agttctgaaa actcatcaat gtaagtcatg
     6781 ctggtgaagt gcgtccattc gcgttcaaac ggatacactg ggatctgaat cagatcttta
     6841 cgctcgacca gatagttgaa cagacgcaat tccatcggtg aaatcacatc ttcggcgtca
     6901 tgcagaataa aaccagcaaa agcgaaattg gcgctacgtt caaactgggt gatggcgtcc
     6961 agcacgttgt tcaggcagtc ggctttgctg gtgggaccag gacgcgcgca gactacctta
     7021 tgcacgttcg gaaagcgagc gcacacttcg tcaacatcac gttgagtatc ggggtcgttg
     7081 gggtaggtgc caacaaagat atgatagttt tcgtagtcga gcgtggtcgc cgccagctcg
     7141 gccatattgc cgatgacgcc cgtttcattc cacgccggaa ccataatcgc taacggtttt
     7201 tcatctggtt tatacagttc gcggtaactc attcgcgggt agcggcgata aacactcaac
     7261 ttgcgtttaa tgcggcgtac ccagtagacg acatcaataa aaaaatcgtc cagcccgctg
     7321 atgaacatga tgaccgctaa cgttatcgcg attactttta agccgtagag ccaggtagca
     7381 aaaacatcaa gaagccagtc cacacaaaaa ccttacatta acgctggtta tgtttagggt
     7441 ggcgtatatt aaggtttttt atgaattgtg acagcttttt accattaata tgtatgacta
     7501 ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat aattaagcgg
     7561 gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc gtgcctttat
     7621 gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca ccttttcgct
     7681 ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc tcgggcgcaa
     7741 tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc tgacagcgta
     7801 ctacaattgt ctctctggtt ggcgtataac gaagggcatt agaaagcagg ttgcttagcg
     7861 cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca aaccgcaact
     7921 ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg acttcatccg
     7981 ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga gcgagaaaca
     8041 gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag tagagcacat
     8101 cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc gtaatgagat
     8161 tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac tggcgagtaa
     8221 agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt tccagctcaa
     8281 tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata ttctggattt
     8341 gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc acgataaaga
     8401 cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa tcatttatgt
     8461 aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt ttgccgtcca
     8521 ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata tgcccgtgac
     8581 cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca ccccgcgcgt
     8641 ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg ggggagtgat
     8701 acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa ccactgacga
     8761 tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt agtacccgtt
     8821 caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa tgcactttta
     8881 ctgagtggat tatgatccat gcaaaggcga aaaacgccgc gatggtggcc aggctgataa
     8941 aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg accatccggc
     9001 acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc aaagtcgttg
     9061 tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc gctgtcgaaa
     9121 ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc ctgatggcgg
     9181 agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt gccgctgagg
     9241 gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg actttcgata
     9301 atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc agcaaaagcg
     9361 aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg atgttcaatg
     9421 gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg caacatgcgc
     9481 acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag gtcataatca
     9541 ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac cacaaaaccg
     9601 gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc gacaatcaac
     9661 agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga tcagatggca
     9721 atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa tcagagcctg
     9781 gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct ccttgtaaac
     9841 ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg gattatcagc
     9901 gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg gttaacgcag
     9961 cggataacta ccagaacgtg ggctggcgca ccttttttgt tgataatcag gtgaagacgc
    10021 tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg aaagtgcagg
    10081 aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc aatggctagg
    10141 gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg gagttctcga
    10201 ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac atgagcgaag
    10261 ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat attttgctgg
    10321 tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa ttgcaaatag
    10381 ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa caactgttga
    10441 ccggtagcag caatgttctg gcgctggaac aggctcgcgg ggtgatagaa agtacccgca
    10501 gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg caactgttat
    10561 tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc ctgcaaagcg
    10621 ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat attatggaag
    10681 ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg gcatttttcc
    10741 cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg tcattattta
    10801 acgctagcag cgggatgtgg aattttattc ctaaaattga gatccccatt tttaatgccg
    10861 gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg gtggtgaatt
    10921 atgaacagaa aatccagaac gcctttaaag aggtggcaga tgcgctggca ttacgtcaaa
    10981 gcctggacga tcaaatcagc gcccagcagc gttatctggc gtcgctgcaa attaccctgc
    11041 aacgggcgcg ggcattatat cagcacggcg cggtaagtta tctggaagtg ctggatgccg
    11101 agcgttcttt atttgcaacc cgacaaactt tacttgacct gaattatgcc cgtcaggtta
    11161 acgaaattta tttgtatacc gccttagggg gcggttggca gcaataattt ttaactccag
    11221 gagagaataa atgaaaaaag cactgcaagt cgcaatgttc agtctgttta ccgttattgg
    11281 ctttaatgcc caggctaacg aacatcatca tgaaaccatg agcgaagcac aaccacaggt
    11341 cattagtgcc accggtgtgg taaaaggtgt tgatctggaa agcaaaaaaa tcaccatcca
    11401 tcacgatccg attgctgccg tcaactggcc ggagatgacc atgcgcttta ccatcacccc
    11461 gcagacgaaa atgagtgaaa ttaaaaccgg cgacaaagtg gcgtttaatt ttgtccagca
    11521 gggcaacctt tctttattac aggatattaa agtcagccag taacccaggt ttaatgagat
    11581 gaaaaaaatc gcgcttatta tcggcagcat gatcgcgggc ggtattattt cagcggcagg
    11641 ttttacctgg tttgaccagg cggaaccgca cacagaaaaa atgccgaccg cagaacgtaa
    11701 aatcttattc tggtacgacc cgatgtatcc caatacgcgg ttcgataaac caggtaaatc
    11761 gccgtttatg gatatggatc tggtgccgaa atatgccgat gaagagagtt ctgcgtctgg
    11821 tgtgcgcatt gacccgactc agacgcagaa tctgggggtg aaaacggcta ccgtcacgcg
    11881 cgggccgctg acttttgccc agagtttccc ggcgaatgtc agttacaacg agtatcagta
    11941 tgccattgtg caggctcgcg ctgccgggtt tatcgacaag gtgtatccgc ttaccgtggg
    12001 cgataaagta caaaagggca caccgcttct cgacctgacc attcccgact gggtggaagc
    12061 gcagagtgag tatttactgt tgcgcgaaac cggcggtacg gcgacccaga ctgaaggcat
    12121 tcttgagcgg ctgcgactgg cgggaatgcc ggaggcggat attcgccacc tgatcgccac
    12181 gcaaaaaatc cagactcgct ttacgctcaa agcgcccatt gatggcgtga tcaccgcgtt
    12241 tgatctgcgc gcgggaatga atatcgccaa agataacgtg gtcgcgaaag aggaaggtag
    12301 gttgcttatc tgtgtctgtc attgtctagc attgtccact gtagttaatt aactgtctgt
    12361 tataactcga aaaacaaccc ttttttcacc tgagtatgtc tacgaacgtc aaatatagtt
    12421 gtttactgtg cgctgttgtc catgtgatat tgtgtaccag atgtgtacca aaccattatt
    12481 tctgagcgta ccaaatatta tttatggcta taagcgacac aaaactgcgt actatttatg
    12541 gtaaaccata ttctggccca caagaagtgg ctgatgctga tggcctcagc gtacgaattt
    12601 caccgaaggg ggtcatccag ttccagtacc gctatcgctg gcatggcaag cctaatcgac
    12661 ttgggcttgg tcgataccca tccctgtctt tgaaggatgc cagacagatc actgctgact
    12721 tgcgaaagct ctatttctca ggaacggatc cacgcactta ttttgaagag aaggtggaga
    12781 actccatgac ggtcgcccag tgtctcgact a
//
LOCUS       DBKELA010000108        12726 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.108, whole genome shotgun sequence.
ACCESSION   DBKELA010000108 DBKELA010000000
VERSION     DBKELA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.108"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACUQR0_003282"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACUQR0_003282"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HHV9110201.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACUQR0_003283"
     CDS             complement(1806..3224)
                     /locus_tag="ACUQR0_003283"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HHV9110202.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALATLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACUQR0_003284"
     CDS             complement(3378..3722)
                     /locus_tag="ACUQR0_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HHV9110203.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACUQR0_003285"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACUQR0_003285"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HHV9110204.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACUQR0_003286"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACUQR0_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACUQR0_003287"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACUQR0_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HHV9110205.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACUQR0_003288"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACUQR0_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HHV9110206.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACUQR0_003289"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACUQR0_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HHV9110207.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACUQR0_003290"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACUQR0_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HHV9110208.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACUQR0_003291"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACUQR0_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HHV9110209.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACUQR0_003292"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACUQR0_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HHV9110210.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACUQR0_003293"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACUQR0_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HHV9110211.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            complement(11206..11307)
                     /locus_tag="ACUQR0_003294"
                     /pseudo
     CDS             complement(11206..11307)
                     /locus_tag="ACUQR0_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACUQR0_003295"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACUQR0_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACUQR0_003296"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACUQR0_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HHV9110212.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3355 a         3258 c         2908 g         3205 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggctagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcac
//
LOCUS       DBKELA010000109        12359 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.109, whole genome shotgun sequence.
ACCESSION   DBKELA010000109 DBKELA010000000
VERSION     DBKELA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.109"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="ACUQR0_003297"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="ACUQR0_003297"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HHV9110213.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="ACUQR0_003298"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="ACUQR0_003298"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HHV9110214.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="ACUQR0_003299"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="ACUQR0_003299"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HHV9110215.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3272..3535
                     /gene="yebG"
                     /locus_tag="ACUQR0_003300"
                     /pseudo
     CDS             3272..3535
                     /gene="yebG"
                     /locus_tag="ACUQR0_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774904.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /gene="yebF"
                     /locus_tag="ACUQR0_003301"
     CDS             3602..3958
                     /gene="yebF"
                     /locus_tag="ACUQR0_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HHV9110216.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="ACUQR0_003302"
     CDS             4285..4944
                     /locus_tag="ACUQR0_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HHV9110217.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="ACUQR0_003303"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="ACUQR0_003303"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HHV9110218.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="ACUQR0_003304"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="ACUQR0_003304"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HHV9110219.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /gene="yobB"
                     /locus_tag="ACUQR0_003305"
                     /pseudo
     CDS             complement(7897..8553)
                     /gene="yobB"
                     /locus_tag="ACUQR0_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="ACUQR0_003306"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="ACUQR0_003306"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HHV9110220.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="ACUQR0_003307"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="ACUQR0_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HHV9110221.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="ACUQR0_003308"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="ACUQR0_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="ACUQR0_003309"
     CDS             10287..10628
                     /locus_tag="ACUQR0_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HHV9110222.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="ACUQR0_003310"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="ACUQR0_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12063..12344)
                     /locus_tag="ACUQR0_003311"
     CDS             complement(12063..12344)
                     /locus_tag="ACUQR0_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HHV9110223.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3062 a         3025 c         3123 g         3149 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcac
//
LOCUS       DBKELA010000110        12219 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.110, whole genome shotgun sequence.
ACCESSION   DBKELA010000110 DBKELA010000000
VERSION     DBKELA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.110"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..492)
                     /locus_tag="ACUQR0_003312"
     CDS             complement(<1..492)
                     /locus_tag="ACUQR0_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HHV9110224.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACUQR0_003313"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACUQR0_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HHV9110225.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACUQR0_003314"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACUQR0_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HHV9110226.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="ACUQR0_003315"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="ACUQR0_003315"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HHV9110227.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACUQR0_003316"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACUQR0_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HHV9110228.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="ACUQR0_003317"
     CDS             3646..4155
                     /locus_tag="ACUQR0_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHV9110229.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACUQR0_003318"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACUQR0_003318"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HHV9110230.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="ACUQR0_003319"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="ACUQR0_003319"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HHV9110231.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="ACUQR0_003320"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="ACUQR0_003320"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HHV9110232.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="ACUQR0_003321"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="ACUQR0_003321"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HHV9110233.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="ACUQR0_003322"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="ACUQR0_003322"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HHV9110234.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYSECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="ACUQR0_003323"
     CDS             complement(8797..9690)
                     /locus_tag="ACUQR0_003323"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HHV9110235.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="ACUQR0_003324"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="ACUQR0_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="ACUQR0_003325"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="ACUQR0_003325"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HHV9110236.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="ACUQR0_003326"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="ACUQR0_003326"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HHV9110237.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2971 a         3156 c         3114 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatgcaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat atcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atcgggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgccg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttaa ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacagtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgcctgcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggagct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtaccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg cagaaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DBKELA010000111        12154 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.111, whole genome shotgun sequence.
ACCESSION   DBKELA010000111 DBKELA010000000
VERSION     DBKELA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.111"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            126..995
                     /locus_tag="ACUQR0_003327"
     CDS             126..995
                     /locus_tag="ACUQR0_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HHV9110238.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACUQR0_003328"
     CDS             complement(1198..1551)
                     /locus_tag="ACUQR0_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HHV9110239.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACUQR0_003329"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACUQR0_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HHV9110240.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACUQR0_003330"
     CDS             complement(3379..3672)
                     /locus_tag="ACUQR0_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HHV9110241.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACUQR0_003331"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACUQR0_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HHV9110242.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACUQR0_003332"
     CDS             complement(5220..5696)
                     /locus_tag="ACUQR0_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HHV9110243.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACUQR0_003333"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACUQR0_003333"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HHV9110244.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACUQR0_003334"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACUQR0_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HHV9110245.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACUQR0_003335"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACUQR0_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HHV9110246.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACUQR0_003336"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACUQR0_003336"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HHV9110247.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACUQR0_003337"
     CDS             11052..11627
                     /locus_tag="ACUQR0_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHV9110248.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACUQR0_003338"
     tRNA            11734..11809
                     /locus_tag="ACUQR0_003338"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac atctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 aaatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caat
//
LOCUS       DBKELA010000112        11923 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.112, whole genome shotgun sequence.
ACCESSION   DBKELA010000112 DBKELA010000000
VERSION     DBKELA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.112"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..2248)
                     /locus_tag="ACUQR0_003339"
     CDS             complement(<1..2248)
                     /locus_tag="ACUQR0_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HHV9110249.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="ACUQR0_003340"
     CDS             complement(2272..2895)
                     /locus_tag="ACUQR0_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HHV9110250.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="ACUQR0_003341"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="ACUQR0_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="ACUQR0_003342"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="ACUQR0_003342"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HHV9110251.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /gene="ubiG"
                     /locus_tag="ACUQR0_003343"
     CDS             7503..8225
                     /gene="ubiG"
                     /locus_tag="ACUQR0_003343"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HHV9110252.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..11923)
                     /gene="yfaL"
                     /locus_tag="ACUQR0_003344"
                     /pseudo
     CDS             complement(8353..11923)
                     /gene="yfaL"
                     /locus_tag="ACUQR0_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2758 a         3308 c         2946 g         2911 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccgga tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 gttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg gcg
//
LOCUS       DBKELA010000113        11801 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.113, whole genome shotgun sequence.
ACCESSION   DBKELA010000113 DBKELA010000000
VERSION     DBKELA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.113"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1330)
                     /gene="ipaHb"
                     /locus_tag="ACUQR0_003345"
     CDS             complement(<1..1330)
                     /gene="ipaHb"
                     /locus_tag="ACUQR0_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HHV9110253.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAV
                     AADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLE"
     gene            1821..1910
                     /locus_tag="ACUQR0_003346"
                     /pseudo
     CDS             1821..1910
                     /locus_tag="ACUQR0_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            2212..3207
                     /gene="pgl"
                     /locus_tag="ACUQR0_003347"
     CDS             2212..3207
                     /gene="pgl"
                     /locus_tag="ACUQR0_003347"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HHV9110254.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(3248..4201)
                     /locus_tag="ACUQR0_003348"
                     /pseudo
     CDS             complement(3248..4201)
                     /locus_tag="ACUQR0_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4385..5437
                     /locus_tag="ACUQR0_003349"
     CDS             4385..5437
                     /locus_tag="ACUQR0_003349"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HHV9110255.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            5513..6946
                     /locus_tag="ACUQR0_003350"
     CDS             5513..6946
                     /locus_tag="ACUQR0_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HHV9110256.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            7129..9309
                     /locus_tag="ACUQR0_003351"
     CDS             7129..9309
                     /locus_tag="ACUQR0_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HHV9110257.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(9450..10733)
                     /locus_tag="ACUQR0_003352"
     CDS             complement(9450..10733)
                     /locus_tag="ACUQR0_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HHV9110258.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(10868..>11801)
                     /locus_tag="ACUQR0_003353"
     CDS             complement(10868..>11801)
                     /locus_tag="ACUQR0_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9110259.1"
                     /translation="EAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASR
                     GIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLS
                     DAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELA
                     VVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKE
                     ILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQI
                     SDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2941 a         2659 c         2946 g         3255 t
ORIGIN      
        1 tttcgaggcg gaacatttcc ctgcccaggg agagcagagc gccggtatca ttatcgaaaa
       61 ggccttcaga tgcctgatgg accaggaggg ttttccggag attgttccat gtgagcgcga
      121 cacggtcctc acagctctca gtggcatcag cagcaacagc gaaagactgc tgtcgaagct
      181 ccgcagaggc actgagtttt tccagccatg cagcgacctg ttcacggaat ccggaggtat
      241 tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc ggagaatgcc cgggaattct
      301 cctcggtgga aaaagaagtc cattgtgtag aaacctcccg acgatctgct tcaggaaacc
      361 aggctgccac agtttcggga agagggggga tctgaaagtt ctgcccccct tcagtgatga
      421 ctctgatatg gcgcgaatgt aaattttgcg gcaacatctg gcgtaaaaac agtctgtttt
      481 ggtctgacag ggaaatacga aactgaggcc cgttaaaatt tggaaccgaa gtttgctgta
      541 ttaagaatag cagagtttga tctgataagg ggttatttgc aatctcaata gttatatgag
      601 gatctaaacg aaaaagattt gtcgggattg tagttatttg gttgttttca aaataaaaaa
      661 atcttctcat gataatattt tcttggatgt ttggtaagtt gcttaatcta ttgctatatg
      721 caaaaagaag ttgcagtctc ctaggtaaag ggggcagttc agtaagatgg ttgtgcgcaa
      781 ccttaagtat cttaagatta gaaggcagag atggaagagt ttgcagttcg ttgttcctaa
      841 cctcaagttt ttttaaattc aagggtaatg aaggcagtgt ggataaccta ttatactctg
      901 cgcttagtga tttgatggtg tctggtaata acggcagatt ctctaattga ttttcggaca
      961 catccaaagt tattagataa tgtggtaatg cagatatatg tgttaagaaa ttatctcttg
     1021 caataagttc tttaatattt tcaggtaaat cagtaaggat ggttaattta ttacagctca
     1081 catcaagctt tttaatgcaa gcagggagta caggtagcga ggaaagcgcc aaggctcgca
     1141 ggtccagttt atgcccctta gaggtaagac atgcacgaag tcttcttacc gcctcagtac
     1201 gctgttcttc cggtaacccc tgtttctccc aatcatccca ttcggacaaa taatggttgg
     1261 gggatgtaat tgtgctgata tgtggaaata aacaggatga aatgtttctg agcatattaa
     1321 tttctcgcat atggatatgg aattagattg tgaataaatt gtatttaatg tatctctcct
     1381 aatgaggagg gtgtttcaga atattcgcca taagggaatg agtgttatat catgctttct
     1441 accctggtaa gtatatttgc ttttttcgta tatttggttc atcttttttt gatgttttac
     1501 atattcacaa tgcagaggat ttataccaga ggacattagt cttgagtaag atactacaac
     1561 atctccaact attttagcca gtctgactac ttgacttggt aatatttgtt gctaataatc
     1621 atgtataaaa taagcttata tacatagtaa cttcagcaaa ggatttagta atgatcaaaa
     1681 tcatatctca atgtactgtt tttttcttta aaaaaacagt acattctatt ttataatgac
     1741 tgtgtattcc catggaagga gatcacccaa ttctcccccc cgacttttga cgcagtgtct
     1801 tgttaacagg gggattgacc atgcttattg gctatgtacg cgtatcaaca aatgaccaga
     1861 ataccgatct acaacgtaat gcgtttattt gtgccggaag tgagttgatt gttgcgcgaa
     1921 tgggaagttg gccagaagtt gttatggcat agtgtttacg gtcgttgata caggtaaaca
     1981 aaagcgggga gagtcatact ccctgaatag tcgcgaaaat atctgtggca ttgtcaaatt
     2041 cgtgtcggtg actaatattt tagtaatatg ccattcgaaa tggagtgcta acttattgat
     2101 ttaattgatg tgcgatattg tattttatcg tgttttgttg tctgtttttt atccagtaag
     2161 tgttgttata tgtatgaaag ataagggatt tatcattttt gaggtgtgtt catgaagcaa
     2221 acagtttata tcgccagccc tgagagccag caaattcacg tctggaacct gaatcatgaa
     2281 ggcgcactga cgctgacaca ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc
     2341 agcccggaca aacgttatct ctatgttggt gttcgccctg agtttcgcgt cctggcgtat
     2401 cgtattgccc cggacgatgg cgcactgacc tttgccgcag agtctgtgct gccgggtagt
     2461 ccgacgcata tttccaccga tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg
     2521 ggtaacgtga gcgtaacgcg tctggaagat ggcctgccag tgggcgtcgt cgatgtagtc
     2581 gaggggctgg acggttgcca ttccgccaat atctcaccgg acaaccgtac gctgtgggtt
     2641 ccggcattaa agcaggatcg catttgcctg tttacggtca gcgatgatgg tcatctggtg
     2701 gcgcaggacc ctgcggaagt gactaccgtt gaaggggccg gcccgcgtca tatggtattc
     2761 catccaaacg aacaatatgc gtattgcgtc aatgagttaa acagctcagt ggatgtctgg
     2821 gaactgaaag atccgcacgg caatatcgaa tgtgtccaga cgctggatat gatgccggaa
     2881 aacttctccg acacccgttg ggcggctgat attcatatca ccccggatgg tcgccattta
     2941 tacgcctgcg accgtaccgc cagtctgatt accgttttca gcgtttcgga agatggcagc
     3001 gtgttgagta aagaaggctt ccagccaacg gaaacccagc cgcgcggctt caatgttgat
     3061 tacagcggca agtatctgat tgccgctggg caaaaatctc accacatctc ggtatacgaa
     3121 attgttggcg agcaggggct actgcatgaa aaaggccgct atgcggtcgg gcagggacca
     3181 atgtgggtgg tggttaacgc acactaaccg ctgatttaac cggcgcagtc tctcctgcgc
     3241 cggtgtatta acttatctcc tgtaacgcat gtctctggcg ttcgacgata ttggtccaca
     3301 aattgtcttt atcgtcagtc cacaaattaa tcagcaagga aaaaaagcgt tcagctgccg
     3361 gagaaagcac ggcatctttg cggcgaataa tccccaatgt ccgacgtata acgggttcca
     3421 ccagtgggat accaataatc gaagaatagg gcgcatgggg cattgccaga ccaggaagcg
     3481 ccgaaatccc cagtcctgcc tccactagtc ctaatgacgt cgaaagatga cgcacttcgt
     3541 aaaaccagtc cagcttccag ggcttgtcgg ctagctgttg ttctatcaac aatcggttgc
     3601 cgctggagga acgtacgcca atcattttgt aacccactaa ctcctgccat tctacaagct
     3661 actttttggc caatggatgg tcacgccgac aggccagcac gaatggctcg ttgaccagtg
     3721 gggtaaaatc aatggatgaa tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt
     3781 tacacagcac cgattccatg caattattcg tgccttgttc cagaatccgc actttaatat
     3841 tgggatacag ctcattaaat ttaccgattg ccagcggtaa aaaataaaat actgcggtcg
     3901 gaatacacgc taatgtcacc atatcacgat gataagcatt catatcacga atattaaaaa
     3961 gcgtttcatc gaatttcttt attaattccc gcgcctccgg gagcaacctt tttcctgctt
     4021 ttgttaacgt aaccttgcga gttgtacgct caaatagttg aatatgtaaa tcttcctcca
     4081 tcttttttat tcttcgcgtt aatgcaggtt gcgtaatatt gagtaattta gcggcattgt
     4141 taaatgaact ggactctgcc agtatgacaa atgccttcat acttgataat tcatgcttca
     4201 ttattactcc ggaaaatgga agcgacgatt ttggggggct ggtcgttaaa aattttaact
     4261 gcatttagcc aacttaaatt aatgaaaaaa tgttattaat cgttgagcta aagtcattag
     4321 agatgctttg tccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat
     4381 tattatgaaa aaaataccct gcgtgatgat gcgaggtgga acctcgaggg ccgcgttcct
     4441 gttagcggaa catttacccg aagatcaaac gcagcgcgat aaaatattga tggcaattat
     4501 gggttccggt aacgatctgg aaattgacgg tattggcggc ggtaatccac tgaccagtaa
     4561 agtcgccatt attagccgtt ccagcgatcc gcgtgctgat gtcgattatc tgtttgctca
     4621 agttatcgtc catgagcaac gtgtcgatac cacgcctaac tgcggcaata tgctgtctgg
     4681 cgtaggggca ttcgccattg aaaatggttt gattgcagcg acttcgccag ttacccgtgt
     4741 acgtatccgc aacgtcaata cgggtacgtt catcgaagct gatgtgcaaa cgccaaatgg
     4801 tgttgtcgag tacgagggta gcgccagaat tgacggcgta ccgggtactg ccgcaccggt
     4861 tgcgctcact ttcctgaatg ccgctggaac gaaaaccgga aaagttttcc cgactgataa
     4921 tcagattgat tattttgacg atgtcccggt gacctgtatc gatatggcga tgccagtcgt
     4981 cattattccg gctgaatatc tgggaaaaac aggttatgaa ttaccggcgg aactggatgc
     5041 cgacaaagca ttattagccc gcattgaatc tatccggcta caagcgggta aagcaatggg
     5101 cttaggtgat gtcagtaata tggttatccc taaacctgtg cttatttctc cagcgcagaa
     5161 aggcggggca attaatgtgc gttattttat gccgcattct tgccatcgcg cgctggcgat
     5221 aaccggtgct attgctattt ccagtagttg tgcattggaa ggcaccgtca cccgacaaat
     5281 cgtcccttct gtaggatatg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt
     5341 tcatttaagt aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacaac
     5401 cagaaaaata ttttccggtg aagtttatct tccctgaaaa aattcgttgt caggataagg
     5461 acaatcaata aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa
     5521 gaaatcgtta tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc
     5581 agctccggca ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat
     5641 tgtgggtctg gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc
     5701 ctcaatggtg gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag
     5761 cggttactct tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt
     5821 tgtgaccacc ggtttaggta aacttattgc ctatctgctg attggtaaaa tcggtaacac
     5881 catgctgggt ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc
     5941 gtctaacacc gcgcgtgcgg gcggcattgt gttaccgatc atcaacagcg tggcgatggc
     6001 tttggggtcc gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat
     6061 ttacatggtc accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct
     6121 ggcgctgaaa atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcgct
     6181 ggccgccgga ttgccgggca tcattatgct gctggtcacc ccgctggtaa tttacaccat
     6241 gtatccacca gaaattaaaa aggtggataa caaaaccatc gctaaagcgg gccttgccga
     6301 actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc tggcgctact
     6361 gggctggatt ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa tcgttgttat
     6421 ggcgaccatg ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg
     6481 ctggaatacc ttaatctggt acggcggtat tatcggctta agctccttat tatcgaaagt
     6541 taaatttttc gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg
     6601 taacgttgct ttcttcgtta ttattttcct cagcatcatc gtgcgttatt tcttcgcttc
     6661 cggtagtgcc tatatcgttg ctatgttacc tgtatttgcc atgctggcga acgtctccgg
     6721 cgcaccgtta atgttaaccg cgctggcact gttgttctcc aactcctatg gcggcatggt
     6781 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
     6841 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
     6901 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
     6961 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
     7021 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
     7081 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
     7141 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
     7201 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
     7261 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
     7321 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
     7381 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
     7441 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
     7501 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctagcgg gggcaaaatg
     7561 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
     7621 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
     7681 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
     7741 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     7801 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     7861 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     7921 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     7981 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
     8041 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     8101 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     8161 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     8221 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     8281 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctcgc caaaaaaggt
     8341 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     8401 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     8461 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgttgatg
     8521 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     8581 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     8641 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     8701 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     8761 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     8821 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     8881 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     8941 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     9001 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     9061 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     9121 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     9181 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     9241 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccgt
     9301 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     9361 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     9421 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     9481 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     9541 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     9601 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     9661 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     9721 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     9781 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     9841 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     9901 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     9961 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacttgg
    10021 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacgct atcgcccagc
    10081 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
    10141 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
    10201 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
    10261 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
    10321 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctccccaggc
    10381 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
    10441 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
    10501 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
    10561 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
    10621 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
    10681 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
    10741 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
    10801 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
    10861 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
    10921 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
    10981 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
    11041 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
    11101 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
    11161 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
    11221 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
    11281 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
    11341 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
    11401 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
    11461 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
    11521 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
    11581 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
    11641 aatttatgag tgtcttctgc ttgattcctc tgctggcgag gattttttcg tagcgatcaa
    11701 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
    11761 tgtgtcctga aaataactca atgttggcct gtatagcttc a
//
LOCUS       DBKELA010000114        11794 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.114, whole genome shotgun sequence.
ACCESSION   DBKELA010000114 DBKELA010000000
VERSION     DBKELA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.114"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            404..2044
                     /gene="trg"
                     /locus_tag="ACUQR0_003354"
     CDS             404..2044
                     /gene="trg"
                     /locus_tag="ACUQR0_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HHV9110260.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2082..3005)
                     /locus_tag="ACUQR0_003355"
     CDS             complement(2082..3005)
                     /locus_tag="ACUQR0_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHV9110261.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3222..4565
                     /gene="hglS"
                     /locus_tag="ACUQR0_003356"
                     /gene_synonym="ydcJ"
     CDS             3222..4565
                     /gene="hglS"
                     /locus_tag="ACUQR0_003356"
                     /gene_synonym="ydcJ"
                     /EC_number="1.13.11.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoadipate dioxygenase/decarboxylase HglS"
                     /protein_id="HHV9110262.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4790..6445
                     /gene="opgD"
                     /locus_tag="ACUQR0_003357"
                     /gene_synonym="mdoD"
     CDS             4790..6445
                     /gene="opgD"
                     /locus_tag="ACUQR0_003357"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HHV9110263.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6585..6809
                     /locus_tag="ACUQR0_003358"
     CDS             6585..6809
                     /locus_tag="ACUQR0_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HHV9110264.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6872..7408
                     /gene="rimL"
                     /locus_tag="ACUQR0_003359"
     CDS             6872..7408
                     /gene="rimL"
                     /locus_tag="ACUQR0_003359"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HHV9110265.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7403..8383)
                     /gene="ydcK"
                     /locus_tag="ACUQR0_003360"
     CDS             complement(7403..8383)
                     /gene="ydcK"
                     /locus_tag="ACUQR0_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HHV9110266.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8507..9499
                     /gene="tehA"
                     /locus_tag="ACUQR0_003361"
     CDS             8507..9499
                     /gene="tehA"
                     /locus_tag="ACUQR0_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HHV9110267.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9496..10089
                     /gene="tehB"
                     /locus_tag="ACUQR0_003362"
     CDS             9496..10089
                     /gene="tehB"
                     /locus_tag="ACUQR0_003362"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HHV9110268.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10392..11060
                     /locus_tag="ACUQR0_003363"
     CDS             10392..11060
                     /locus_tag="ACUQR0_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HHV9110269.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11078..11584)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_003364"
                     /pseudo
     CDS             complement(11078..11584)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11592..>11794
                     /locus_tag="ACUQR0_003365"
     CDS             11592..>11794
                     /locus_tag="ACUQR0_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9110270.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATET"
BASE COUNT         3053 a         2741 c         2958 g         3042 t
ORIGIN      
        1 tttcaaaagg gtattttggc tttgacatat taggggctat tccatttcat cgtccaacaa
       61 aatgggtgca gtacagttga tagaaatatc ggtcggggcc ttcgtctttc tgattcccgg
      121 ttagcctgaa aacagaaagt ctcaggcacc cgcaggcatc ctatgaggtt tccttaggga
      181 cgaaaataat cacttcacga aattgcgtgc tgttttccag aatttttcgt cattcgggtt
      241 agccagttta gccattcgtt actctcttca ttccaatagc attaattttc tctgcaataa
      301 ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa gtaattaccg tcaagtgccg
      361 atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc
      421 acagcgatta ggttttttgc atcacatcag gctggttccg ttatttgcct gcattcttgg
      481 cggtatctta gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc
      541 cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag
      601 ttcagatttt ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat
      661 tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc
      721 gcagcaaggt tatcgtgctt atcagaatcg accggtgaaa acacctgctg atgaagcctt
      781 cgacactgaa ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa
      841 atatgccaaa aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc agatccgacc
      901 gctggataat gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc
      961 caaccaactg gcggaactgg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg
     1021 cgcgtttgtg cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt
     1081 cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac
     1141 gatgaatgat gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca
     1201 aatgcagcat tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta
     1261 tcgtggcacc agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca
     1321 agcggcggct atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca
     1381 gaatgcggat aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag
     1441 cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc
     1501 gaagaaaatt tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct
     1561 ggcactgaat gctgccgttg aagccgcgcg agcgggtgag caagggcgtg gatttgccgt
     1621 tgtcgccagc gaagtacgga cactcgcaag ccgcagcgct caggcagcga aagagattga
     1681 aggcttgatc agtgaatcag tcaggttaat tgacctgggg tcggatgagg tggcaacggc
     1741 agggaaaacc atgagcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga
     1801 aatcgccgcc gcctcggatg aacaaagtag aggcataact caggttagcc aggcgatttc
     1861 tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcagcggc
     1921 ggtgtccctt gaagaacagg ctgcacgatt aactgaggcg gtgaacgtat tccgtctgaa
     1981 caaacattct gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt tcgctacggt
     2041 gtgaaaatgt tcaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt
     2101 cgaatagcgt taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt
     2161 attccaatcg gttcgcctgc accatgtccc ggaacaggca gggccaccag cgtggcatga
     2221 cgcaggtcgt cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaacggta
     2281 agttgacgag atagcgaagt agacagcgtt tcgatacaac ccgaaggaat tttacagccc
     2341 tggctctgca ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg
     2401 accggccatt ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattaggg
     2461 cggacaacca gcttcaacga ttcaagaaac agcagttcgt aattaagccc ggtcatcagt
     2521 tcaggatctg acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga
     2581 atcatagggt tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac
     2641 tgacctataa ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc aaccctgacg
     2701 acatcattat taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca
     2761 agaactctga ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga
     2821 cgaccacgct caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtattagag
     2881 agcgcaggtt gactcaaatt aagggtttca gctgcgcgcc ccaaagttcc ttgttgtgcg
     2941 acagctacga atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt
     3001 tccataagcg atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt ttgattatga
     3061 taacttgatt gcaaaatatt attaataatt aaagcaatta tgttacagca aaatggataa
     3121 tattgatgtt ttcgcggcga gatcacagtt tgtaaattct tcccgccaga gtgaatgcgg
     3181 ttacctacac tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg
     3241 cggatgagat tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc
     3301 cgcaatatgg cacgctgctg gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca
     3361 atcctcaact gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac
     3421 gtcatggggc gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg
     3481 ccattatggg gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac
     3541 attcgacagc atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt
     3601 ttacatcctt actccgcctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg
     3661 agattctgcg tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg
     3721 agcagcaggg cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa
     3781 cgtttcgctg gcaccagtta gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg
     3841 aacatcggtt gattgctgat gtggtctgtt ttcctggatg ccatatcaac cacctgacgc
     3901 cacgtacgct ggatattgac cgggtgcagt cgatgacgcc tgaatgcgga attgaaccca
     3961 aaattctcat cgaggggccg ccgcgccgcg aggtaccgat tttactacgc cagaccagct
     4021 ttaaagcact ggaagagacg gtgttgtttg cggggcagaa acagggcacg cataccgcgc
     4081 gctttggtga aattgagcag cgtggcgtgg cattaacgcc gaaagggcga caactgtatg
     4141 atgatcttct gcgtaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac
     4201 aggaaacctt ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggttggcat
     4261 ggttccggta ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcctggag
     4321 acgatccaca gcccttaatt gaacgtggtt gggtagtggc gcaacccatc acctatgaag
     4381 atttcttgcc cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcaga
     4441 cacgcagtca cggtaatgcc agtcgcgaag catttgagca ggcgttgggt tgtccggttt
     4501 tggatgagtt ccagctttac caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc
     4561 tttaaattct gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct
     4621 catcccatcg acataaaaaa aatgccgatt tatgcatatt ctctcagttc aacaattgga
     4681 ttattaataa atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa
     4741 acgaccagcg cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag
     4801 acgatttatt aaaggtttaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc
     4861 tcttttttct caggcagcat tcgcggcaga ttctgatatt gccgacgggc aaacccagcg
     4921 ttttgacttc tccattctac agtcaatggc gcacgactta gcgcaaacag cgtggcgtgg
     4981 tgcgcctcgt ccgttacctg acacgctggc gacaatgacg ccgcaggctt ataacagtat
     5041 tcaatacgac gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca
     5101 gttcttccat atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc
     5161 aacacatctg gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcagg
     5221 tgttgatacc aaacaattag aagggcaaag cgatctcggc tttgccggtt ttcgcgtgtt
     5281 taaagccccc gaactggcgc gccgtgatgt agtatcattt ctcggcgcga gttatttccg
     5341 cgccgttgat gatacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta
     5401 caccgacagt aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc
     5461 gggggcaact acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta
     5521 taagttcact atccattgtg agaaaagtca ggtgattatg gatgtggaaa atcacctgta
     5581 tgcgcgcaaa gacattaaac agctgggcat ttcgccgatg accagtatgt tcagctgcgg
     5641 tactaatgaa cgtcggatgt gcgatacaat tcatccgcaa attcatgact ctgatcgtct
     5701 gtccatgtgg cggggcaacg gcgagtggat ttgccgtccg ctgaataatc cgcaaaaatt
     5761 gcagttcaat gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg
     5821 tgacttctcc cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt
     5881 ggaaccgcgt aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg
     5941 cgaaacgctg gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcaggtga
     6001 tgagtttgca ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt
     6061 agcgcgcgtt atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg
     6121 tgaacactat cccgaaaaat gggcgcgtcg ttttgccgtc gatttcgttg gtggtgatct
     6181 gaaagctgcc gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa
     6241 gcaaatcgaa attctctata ttgaacccat cgatggttat cgtattcagt ttgactggta
     6301 tccgacttcg gactccactg atccggtcga tatgcggatg tatctacgtt gtcaggggga
     6361 cgctatcagt gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta
     6421 tgttgacgac cgcgtgatga gttaatcgtt ttttcttcgg caccttcttc gggaggtgcc
     6481 gtctggttaa acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt
     6541 gtgtatttca gatacaccgt gtcagccact aacagggagt gcgtatgttt ccagaatacc
     6601 gagatttaat atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac
     6661 acaataaact tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg
     6721 cggaagtggt ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc
     6781 tgcagcagga gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt
     6841 ttaacaacag catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc
     6901 acttgaatta catgctgttg cggaaagtca cgtcacacct ctttatcagt taatctgtaa
     6961 aaataaaacc tggttacagc agtcgctaaa ctggccgcag ttcgttcaga gtgaagagga
     7021 cacgcgaaaa acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt
     7081 catgattttc aaagaagatg aacttatcgg cgttatctcg tttaatcgta ttgaaccact
     7141 gaataaaact gctgaaatcg gctactggct ggacgaatct catcaggggc aggggatcat
     7201 ttctcaggcg ctgcaggcat tgattcatca ttacgctcag tctggtgaac ttagacgctt
     7261 cgtgatcaaa tgtcgggtgg acaatccgca aagcaaccag gtcgctttgc gcaatggttt
     7321 tatccttgaa ggttgcctga aacaggctga gttcctgaat gatgcctatg atgatgtgaa
     7381 cttatacgcg cgtattatcg attcataata accctgccag cggcgttcgc gtaatgcgct
     7441 cctcgccgtt aatgactttt ggaccacgga catgaaccgt gtcgccatca aaggcttcaa
     7501 cgacggcgtg gtcggttaac tccacatggt tttcaattat caccgcacct gtaatacggc
     7561 tctcaccttg tattacaacg tgctcgtcga gcagaatcgg tccaccacgt accacggcat
     7621 ttccgccaat caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg
     7681 ccacctgcga actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct
     7741 gcgcgtgacc gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt
     7801 caacactggc aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg
     7861 catcgccata aatttgtgcc tggtggacaa tacgcgaggc gctaaccctt gcgcgatcat
     7921 aaatttggag caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat
     7981 gttgatcaat taaggcgtga ccaaatattc tacattgcac gtaaactaat gagtcatgaa
     8041 tggtgacact gtcacttata tatgcaccct ggctgatttc actattatcg atccagacgt
     8101 tatccgttgc gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattaccgg
     8161 aaatcaccgt tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct
     8221 gtgctaacac cgtttcgcga tcgatccagc cgccagcggt tcctgctatc acatcgttaa
     8281 aatcgctaat ggcgataatc tgccgtaata acacactttt tttagtgcca tcttcctgat
     8341 aactaaaggc tcgctgttct tcacttaggc gatatttgcg catagcgttt tcccacaggt
     8401 gacttactat aaccgtagca aattctgcgg ctctggctat gctcgagaaa attccataaa
     8461 atgcatttca aatatacttt ataaattaaa caaaatgagt aagaagatgc agagcgataa
     8521 agtgctcaat ttgccggcag gctactttgg tattgtgttg gggacgatag ggatgggatt
     8581 tgcctggcgc tatgccagcc aggtttggca ggtcagccac tggttagggg atgggctggt
     8641 gattctggcg atgatcatct ggggattatt gactagcgca tttattaccc gactcatacg
     8701 ctttccgcat agcgtgctgg cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt
     8761 gtttccggca acgacgatgc tggtggcgat tggttttgtt ccgtggtttc gcccactggc
     8821 ggtgtgcatg ttcagttttg gtgtcgtggt tcagttggct tatgccgcct ggcaaactgc
     8881 gggattatgg cgcggatctc accctgaaga agctaccacg cctggactgt atctgccgac
     8941 agttgccaac aactttatca gcgcaatggc ctgtggtgcg ttgggctaca ccgacgccgg
     9001 tctggtgttt ttaggcgcag gcgttttctc atggctaagc cttgaaccgg tgatcttgca
     9061 gcgtctgcgc agttcgggag aattacccac ggcactgcgg acatcactcg gcattcagct
     9121 cgctcctgcg ctggtggcct gtagtgcctg gctgagcgtc aacggcggcg agggtgacac
     9181 gctggcgaaa atgcttttcg gttatggact gctgcaactg ctgtttatgc tacgtctgat
     9241 gccatggtat ctctcccagc catttaatgc ttcattctgg agtttctcgt tcggcgtatc
     9301 tgcactggca accaccggtt tgcatctggg gagtggcagc gataatggat ttttccatac
     9361 gctggcggtg ccgctgttta tctttaccaa ttttattatt gcaatactgc tcatccgtac
     9421 ttttgcgctt ctgatgcagg gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa
     9481 agcagaggac aaagaatgat cattcgtgac gaaaactatt ttactgataa atatgaatta
     9541 acccgcacac actctgaagt actggaagcg gtgaaagtgg ttaaaccggg taaaacgctg
     9601 gatctgggct gtggcaatgg tcgtaacagt ctttacctgg cagccaatgg ttatgatgtt
     9661 gacgcatggg ataaaaatgc catgagtatc gccaacgtcg agcgcattaa atccattgaa
     9721 aatctggata atttacacac ccgggtcgtt gatctgaata acctcacatt tgatggacag
     9781 tacgatttta ttctttcgac tgtggtgctg atgttccttg aggctaaaac catcccaggg
     9841 ttgattgcca atatgcaacg ttgcactaaa cctggtggtt acaacctgat tgtggcggcg
     9901 atggataccg ctgattatcc atgtaccgtc ggcttcacgt ttgccttcaa agagggagaa
     9961 ttacgtcgat attacgaagg ctgggagagg gtgaaataca atgaagacgt cggcgagctg
    10021 caccgcaccg acgccaacgg taatcgtatt aaactgcgtt tcgccacgat gctggcacgt
    10081 aaaaaatgac ccggtaagca caaaacgcgt gaaaattccc cacgctgaga tgatttactg
    10141 ttcttctttt cggtaagcat attttttatc gaagggatgt gaaattaatc acagtagtcg
    10201 aagtttttta gcagcttaac ttactgaaat ttaactactg atgattgact tagccccttt
    10261 ttcggcattg actatgtcgt ctgaaaaggg gctgaaaaat ttattttcac caacactttt
    10321 tttgccacaa cacgaagcgg cgctttttgc tataacttag aaagtaatat aatcatctca
    10381 ggaaactatt catgcgtacc acatcatttg cgaaagttgc agctttatgc ggcttgttgg
    10441 ctctgtctgg ttgtgcctct aaaatcaccc agccagataa atattctggt tttttaaaca
    10501 attactctga tttaaaagaa acaacctcag caacaggtaa acctgtttta cgttgggtag
    10561 acccgagttt tgatcaaagc aaatatgaca gcatcgtctg gaacccaatc acttattatc
    10621 cggtaccaaa accttctacc caggtagggc agaaagttct ggataaaatt ttgaactata
    10681 ccaacaccga aatgaaagaa gcgatagcgc agcgtaaacc agtggttacc accgctgggc
    10741 cgcgtagtct gattttccgt ggggccatta ccggtgtaga taccagcaaa gaagggctgc
    10801 aattctatga agtggttcct gttgcattag tggttgcggg gacgcaaatg gctacaggcc
    10861 accgtaccat ggatactcgc ctctattttg aaggtgagct gattgatgca gcgactaata
    10921 aaccggttat caaagtcgtt cgtcagggcg aaggtaaaga cctgaataac gaaagtacgc
    10981 caatggcttt cgaaaatatt aaacaagtta ttgatgacat ggcgaccgat gccaccatgt
    11041 ttgacgttaa caaaaagtag ttccagacgc gccatcgtta gatggcgctt tttatccggt
    11101 gcgccgtaaa accccatcct tcagggcggg gatataaggc gcggttttcc acctgaccag
    11161 gtgtttgctg ttgttcaatg tattggcgga tgacggatat tggcgcacct ccgcagctac
    11221 tggcaaaata gccaggactc cacaaaacgc ctttgtagta atacctgact gcaatatctg
    11281 gtcgatctcg tcgcagtaac ctacccgata cgcctttgag gctgtttacc agactggata
    11341 tcgccagttt gggaggttag ttaattaaca aatggacgtg atctggttcg ccatccattt
    11401 caaccagttc agcttcaaaa taagcacata catttgaaaa gtaagtgcgt agtttttctg
    11461 tcgcgtcatg gtcaaaaatc tggcgtcggt atctggtgac aaagactagg tgaacatgca
    11521 tcaggaaaac acaatgcctt ccacgccgga tatcggtttc ttttttcaca gaccaaagta
    11581 tcatttgacc tgtgaaacga ttacaggcat ttaaattcca gttaagacca ggtggtcaac
    11641 aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg
    11701 cacgtcagaa tgagaatcat gaggccggta ataaatacat cccttacggg aaaatggctt
    11761 cctggctggt tgagtggaaa aatgccactg aaac
//
LOCUS       DBKELA010000115        11631 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.115, whole genome shotgun sequence.
ACCESSION   DBKELA010000115 DBKELA010000000
VERSION     DBKELA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.115"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(282..357)
                     /locus_tag="ACUQR0_003366"
     tRNA            complement(282..357)
                     /locus_tag="ACUQR0_003366"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="ACUQR0_003367"
     CDS             916..3168
                     /locus_tag="ACUQR0_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HHV9110271.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="glaH"
                     /locus_tag="ACUQR0_003368"
     CDS             3504..4481
                     /gene="glaH"
                     /locus_tag="ACUQR0_003368"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HHV9110272.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="ACUQR0_003369"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="ACUQR0_003369"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HHV9110273.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="ACUQR0_003370"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="ACUQR0_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="ACUQR0_003371"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="ACUQR0_003371"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HHV9110274.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="ACUQR0_003372"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="ACUQR0_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HHV9110275.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="ACUQR0_003373"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="ACUQR0_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HHV9110276.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="ACUQR0_003374"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="ACUQR0_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HHV9110277.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="ACUQR0_003375"
     CDS             complement(11389..11547)
                     /locus_tag="ACUQR0_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HHV9110278.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2992 a         2855 c         2900 g         2884 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggctacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgctacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcagcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag t
//
LOCUS       DBKELA010000116        11427 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.116, whole genome shotgun sequence.
ACCESSION   DBKELA010000116 DBKELA010000000
VERSION     DBKELA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.116"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..630
                     /locus_tag="ACUQR0_003376"
     CDS             <1..630
                     /locus_tag="ACUQR0_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/Tn5 family transposase DNA-binding protein"
                     /protein_id="HHV9110279.1"
                     /translation="KQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELK
                     TSKNTRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYD
                     LFERAAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDG
                     RGQQARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(580..1773)
                     /locus_tag="ACUQR0_003377"
     CDS             complement(580..1773)
                     /locus_tag="ACUQR0_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9110280.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1909..3633
                     /gene="iucA"
                     /locus_tag="ACUQR0_003378"
     CDS             1909..3633
                     /gene="iucA"
                     /locus_tag="ACUQR0_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HHV9110281.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3634..4581
                     /gene="iucB"
                     /locus_tag="ACUQR0_003379"
     CDS             3634..4581
                     /gene="iucB"
                     /locus_tag="ACUQR0_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HHV9110282.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4581..6323
                     /gene="iucC"
                     /locus_tag="ACUQR0_003380"
     CDS             4581..6323
                     /gene="iucC"
                     /locus_tag="ACUQR0_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HHV9110283.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6320..7657
                     /gene="iucD"
                     /locus_tag="ACUQR0_003381"
     CDS             6320..7657
                     /gene="iucD"
                     /locus_tag="ACUQR0_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HHV9110284.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7663..9858
                     /gene="iutA"
                     /locus_tag="ACUQR0_003382"
     CDS             7663..9858
                     /gene="iutA"
                     /locus_tag="ACUQR0_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HHV9110285.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10409..10552)
                     /locus_tag="ACUQR0_003383"
     CDS             complement(10409..10552)
                     /locus_tag="ACUQR0_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110286.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10802..>11427
                     /locus_tag="ACUQR0_003384"
     CDS             10802..>11427
                     /locus_tag="ACUQR0_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HHV9110287.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFING"
BASE COUNT         2873 a         2855 c         2950 g         2749 t
ORIGIN      
        1 aaacaaggag ttggtcccaa aaaacataaa gatgaaagaa acttatttct tcatcctcaa
       61 ctcgtcatca gtgagtcagg tgtttgcctc ggtgtttatg atgactatca gtggttcagg
      121 gatgaattaa aaaccagtaa aaatacccga caagaaatct gcaatgattt acttcataaa
      181 aaacatgtca gtgaaaaaga aacctggcgt tgggttgaag gatataataa agcaacagaa
      241 ctggctcgcc aatgccctga tacccatgta ttgtcaattt ctgatcgtga aggcgatttt
      301 tatgacctgt ttgaacgcgc agcgcagact cccggaataa aagctgactg gcttgtcaga
      361 atgaaattta ataacagagc aacgctgaat attaatggta aacgggatca taggttgttg
      421 cacgaaagaa tcatggagat tacaccgcag caactggttg aattcactat acctgatggg
      481 cgggggcagc aagccagaac actgcctgac atgctttcat cagacgcagg tcctctctca
      541 tatcctctga tttactccag taaggtgatg atcatatctt tatttgatag tacggtaatg
      601 ccagataaat acagacacaa tggtaaataa tatagttgca aataatgctg ccataatcag
      661 cgcgattgcg acacctgttt tcgataccag cggcaccagc aaagcgcaca gtccatagcc
      721 aagtgtgtga ctggtagcga gccagcccgc gcctgcgcca tcagccagct tatcattcag
      781 tagaagttga taagcgggtg ttgccagagc tgctccaaat gacagcactg cacagcccgg
      841 gtaaaacaac catagctgtt cggagagcat gatagccaga ccaccactca tcaacactcc
      901 ggcactcagg agcagggcca ccggagtcag gcgctgcgga cggagtaccc cgaactgcgc
      961 gataagcgca gctactgcgg acagacccaa cagccaagcc acctgttggc taatggcggt
     1021 ggtatccgta gcgaactggc gagtaagggc aggcgaaagt ccaagctgca tcatgctcac
     1081 cgccgcagcc agtaatattg cgcaaagcag ataaggcaga caatcccgct tcagactgac
     1141 gctcttgcac tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagca gcatcagcag
     1201 cgccagcact ggggctgcca tcagcagccc cagtggcgcc agaggatgaa tggccagcat
     1261 tgctgccgcg cacagcggcc cgaatagcct gccgcaactc aggccggagc tgatggttgc
     1321 cagagcggcc atgcgattcc cttctccagc tctctgcaat gcccagacct gacaggctgg
     1381 caccattgcc gacacggtca gcccgtaggc gatccgggcg atgattaata ttcccagtcc
     1441 caccgctgtt gttatggcgc tggtagccat cagcacgctt cccagcccta gcagggtaaa
     1501 gcttatgcca tatcctccca gagcccacaa caccacaggt ttactgccag tacgggaaat
     1561 ttgctttccc caccacgggg acgatggcag aaacagcatt gagccaagca tcagcagggc
     1621 agcccataca gacagactca gatttgtctg tatgaccaga acagggagca caaccagcag
     1681 accgttctgt ccgataccga gaagcccggc actgaacgca agtggccagc aggacagtgg
     1741 tttttggggc gtatcttcga tccctggtga cattataatg tttcaactcc atgtattaat
     1801 tgtgtttatt tgtaaaatta atttatctga caataacatt tattgttgat aatgagaatc
     1861 attattgaca taattgttat tattttactg tgtgggagct gtttgactat gaccctgccc
     1921 tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact gattcgtgaa
     1981 accacagact ggaaactgac agaatacccg ccagacgaat tgctcatccc gctggatgag
     2041 cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca ccgctttgca
     2101 tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac cactctctcc
     2161 cggcttatta ttgataagct acgccatcaa ctgtttctgc ccgttcccct ctgcgaaact
     2221 ttccaccagc gcgtgctgga aagccatgtc catacgcaac aggcaattga tgcccgtcat
     2281 gactgggccg ctctgcgtga aaaagcgttg aattttggcg aggctgagca ggcgctgctg
     2341 acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg gcgggaggct
     2401 gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc ggtggataaa
     2461 acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac gcgatttgcc
     2521 gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct gttcccgttg
     2581 cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact tgttgctaaa
     2641 gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac ctcttcccgt
     2701 tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt acggctgacc
     2761 aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct ggcacgcctg
     2821 gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg ggtaatgcag
     2881 gaggatggct gggccgggct gctcgatctt aacggcaaca tcatgcagga aagtctgttt
     2941 gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agactaacgt actggtctcc
     3001 ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt aaaacgcctg
     3061 agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggttga tgcatactgt
     3121 cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt gctgctggcg
     3181 catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt tatttaccgt
     3241 gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc tattggcgag
     3301 gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc ttattacctg
     3361 ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct ggacagcgaa
     3421 tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca ggtgactcat
     3481 aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg taactttttc
     3541 tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat ctatttcgat
     3601 ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat tattcacagc
     3661 agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg gggactggac
     3721 aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg cgatgcgctg
     3781 gatcagatat ttatcgccgc accacaactg tcagcagtgg ctctcccctg ggccgaatgg
     3841 cgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat tatccatcgt
     3901 actgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc ctccggtgaa
     3961 atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg tccacagggt
     4021 gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg cattgccgat
     4081 cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt tgagtatttc
     4141 tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca gctcaccggt
     4201 aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta tttcgaaatc
     4261 tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt tgaccgtggc
     4321 ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca aagctggctg
     4381 cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac agtactggag
     4441 ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata ccggacaatt
     4501 aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg tcatcacttc
     4561 ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac cgtcggctgg
     4621 tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa tctcagggcg
     4681 atgaccacta ctgcattaac ctgccgggag cacaatggcg cttcatcgct gaacgtggta
     4741 tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa ccagtactgg
     4801 cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc actgttgcag
     4861 agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg aaggcccgtc
     4921 gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa tgcctgctga
     4981 gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa gaggcgctgg
     5041 aacgatatgc gccagagtat accaacacct tcagactgca ctggctggcg gtaaaacgtg
     5101 aacatatgat ctggcgctgc gataacgatc tggatattca gcagttgttg actgccgcaa
     5161 tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacggg ctggatcaca
     5221 actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgcc acagacttca
     5281 tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac cagtggctcg
     5341 cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg gggagggctg gatatcaagc
     5401 tgccgctgac catctacaat acctcatgct accgggggat ccctggcaga tatatcgctg
     5461 ccggaccact ggcttcacgc tggctgcaac aggtttttgc gaccgacgcc accctggtgc
     5521 aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta tgtgtcccat gaaggctatg
     5581 ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc tggcgggaga
     5641 atccgtgccg ctggctgaaa ccggatgaaa gtcctgttct gatggcaaca ctgatggagt
     5701 gcgacgaaaa caatcagccg ctggcaggcg catatatcga ccgctccgga ctggacgctg
     5761 aaacctggct tacgcaattg ttccgggtgg ttgtggttcc gctgtatcac ctgctttgcc
     5821 gctacggtgt cgcgcttatt gcacacggac aaaatataac gctcgccatg aaagaggggg
     5881 taccacagcg tgttctgctg aaagacttcc agggcgatat gcggctggtg aaagaagcgt
     5941 ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt cacatcccgt ctgagtgcgg
     6001 actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt tttatttcgc
     6061 cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg gcagcagtgt
     6121 tgagtgatta catgaacaaa catccacaaa tggcagagcg ttttgcgctt ttctcactct
     6181 tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg ccggacctgg
     6241 atggcggcag tcgcatgctg ccgaattacc ttgagaatct gcaaaatccg ctgtggctgg
     6301 taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtcg ggacagggcc
     6361 atttaatctc agcattgctg cattgtcaca tcagatcgaa gaactgaact gtctcttctt
     6421 tgacgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt gtcatatgca
     6481 gaccgtcttt ctgaaagatc tggtcagtgc agttgcacct acaaatccct acagctttgt
     6541 taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcaggc tacgtacagt
     6601 atcccgtgaa gagttttctg actatctccg ctgggctgct gaagatatga ataacctgta
     6661 tttcagtcat accgttgaaa atattgattt cgacaaaaaa agtcgattgt ttctggttca
     6721 gaccagtcgg ggagaatatt ttgcccacaa tatctgcctt ggtacaggaa aacaacctta
     6781 tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc catgccagtg aaatgaatct
     6841 tcgtcggccg gacctgagtg gaaaacggat aaccgtggtt ggtggaggac agagtggtgc
     6901 agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa taaactgggt
     6961 ctcacggcgt aataatttta acgcactgga tgaggctgct tttgctgatg agtattttac
     7021 acctgaatat atttcaggct tctccggact gaaggaagat attcgccatc agttactgga
     7081 tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca tttatcgtga
     7141 gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc tacccagtcg
     7201 ctcggtaaca actctggaaa gtagtggtcc gggctggagg ttattgatgg aacatcatct
     7261 ggatcagggc agagagagcc tggaaagtga tgtggtgatt ttcgccacag gttaccgttc
     7321 tgcattgcca caaatacttc cctcactgat gcccttgatc accatgcacg ataagaacac
     7381 ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaaa ataacatctt
     7441 tgcggtcaac gccagcatgc aaactcatgg catcgccgaa ccccagctca gcctgatggc
     7501 ctggcgatct gcacgtattc ttaatcgcgt actggggcgt gatttattcg atctcagtat
     7561 gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa aaaccacagc cggaggctgc
     7621 ttctttaact cactatacag caaatattca ggaataacga tgatgcgcaa aaagtatatg
     7681 ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac cagctgtcgc tcagcaaaac
     7741 gatgataatg agatcatagt gtctgccagc cgcagcaatc gaactgtagc ggagatggcg
     7801 caaaccacct gggttatcga aaatgccgaa ctggagcagc agattcaggg cggtaaagag
     7861 ctgaaagacg cactggctca gttaatcccc ggccttgatg tcagcagcca gagtcgaacc
     7921 aactacggta tgaacatgcg tggccgcccg ctggttgtcc tgattgacgg tgtgcgcctc
     7981 aactcttcac gttccgacag ccgacaactg gactctgtcg atccttttaa tatcgaccat
     8041 attgaagtga tctccggcgc gacggccctg tacggtggcg ggagtaccgg agggttgatc
     8101 aacatcgtga ccaaaaaagg tcagccggaa accatgatgg agtttgaggc tggcacaaaa
     8161 agtggcttta acagcagtaa agatcacgat gagcgcattg ccggtgctgt ctccggcgga
     8221 aatgaccata tctccggacg tctttccgtg gcatatcaga aatttggcgg ctggtttgac
     8281 ggtaacggcg atgccaccct gcttgataac acccagaccg gcctgcagca ctccaatcgg
     8341 ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct tcaactgata
     8401 acgcagtact ataaaagtca gggagacgac aattacgggc ttaatctcgg gaaaggcttt
     8461 tccgccatca gcgggagcag cacaccatac gtcagtaagg ggctgaattc tgaccgcatt
     8521 cccggcactg agcggcattt gatcagcctg cagtactctg acagtgattt cctgggacag
     8581 gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc ggtactaccc gttcccgacg
     8641 gtaaatgcga ataaacaggc gacggctttc tcctcgtcac agcaggatac cgaccagtac
     8701 ggcatgaaac tgactctgaa cagccaactg atggacggct ggcaaatcac ctgggggctg
     8761 gatgctgagc atgagcgctt tacctccaac cagatgttct tcgatctggc tcaggcaagt
     8821 gcttccggag ggctgaacaa ccataagatt tacaccaccg ggcgctatcc gtcatatgac
     8881 atcaccaatc tggcggcctt cctgcaatcc agctatgaca ttaatgatat ttttaccgtt
     8941 agcggtggcg tacgctatca gtatactgag aacagggtag atgatttcat cgactacacg
     9001 cagcaacaga agattgctgc cgggaaggcg atatctgccg acgccattcc tggtggttcg
     9061 gtagattacg ataactttct gttcaatgct ggtctgctga tgcacatcac cgaacgtcag
     9121 caggcatggt tcaatttttc ccagggggtg gcattgccgg atccggggaa atattatggt
     9181 cgcggcatct atggtgcagc agtgaacggc catcttcccc tgacaaagag cgtgaacgtc
     9241 agcgacagta agctggaagg cgtgaaagtc gattcttatg aactgggctg gcgctttatc
     9301 ggtgacaacc tgcggactca aatcgcggca tattactcgc tttccaataa gagcgtggaa
     9361 aggaataaag atctgaccat cagtgtgaag gacgacaggc gccgtattta cggcgtggaa
     9421 ggtgcggtgg actacctgat cccggatact gactggagta ccggtgtgaa cttcaatgtg
     9481 ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat atgacgtgaa ggaatcaagt
     9541 ccatcgaaag cgacagctta cattaactgg gcgccggaac cgtggagtct gcgtgtacag
     9601 agcaccactt ctttcgacgt aagcgatgca gagggtaacg atattaatgg ttacactacc
     9661 gtcgatttta tcagtagttg gcagcttccg gtgggaacac tcagcttcag cgttgagaac
     9721 ctcttcgacc gtgactatac cactgtctgg ggacagcgtg cacctctgta ctacagcccg
     9781 ggttacggcc ctgcttcact gtacgactac aaaggccggg gccgaacctt tggtctgaac
     9841 tactcagtgc tgttctgacg gggatgcttt tgcaacaagg gtatgctgta aacaacatgg
     9901 tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa gatattccgt
     9961 ctccggaatg ggctgttcac cactgaataa cattatgtct gttttatgga tctttgactg
    10021 aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac aagtaagcgt
    10081 cgtctcagca ccgtctggca gatcctgaaa ttcctgagag gatgatctgc gcccaatatc
    10141 ctgagccgga actttgctgc aatgaaggcc aacgaaaaat gggggagtat gttatagagt
    10201 acttgctaca gggtaaaaag aggtacctgt cgctggttct ccgttcagtt taaccgggag
    10261 attttctcct gcgcctgtca taaagtccgg taatggagat tgttaatacg atggtgcacg
    10321 atgcatcgtc agtgcccggc ccggaggatg ccccgtagct acatacggat cagggttggc
    10381 aatatcgaat ggcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc
    10441 gcacaaagga aacggtctga ataacgcagt gatggagaac ttcttcggca cactgcaaaa
    10501 tcgcatcgtg gtcgatcgct aattcgtcgt gaatcactga acgatgttcc atgcgaacag
    10561 atattcgatc atatcgaaat attaaacatt acccctcccc acattgggca aaaaaaaaca
    10621 aaaaacgatc aattaacgat caattagtat tgattcacat aatctctcat cattaatgag
    10681 aacattgtca catatttaca atatagaact gttactttta gcaagctgtt agtaaccaac
    10741 cgttactaat aattttaatt cttaatactt tgttcattca ataaatgagt tgagagaata
    10801 tatgaataaa atatactccc ttaaatatag tgctgccact ggcggactca ttgctgtttc
    10861 tgaattagcg aaaagagttt ctggtaaaac aaaccgaaaa cttgtagcaa caatgttgtc
    10921 tctggctgtt gcaggtacag taaatgcagc aaatattgat atatcaaatg tatgggcgag
    10981 agactatctt gatcttgcac aaaataaagg tattttccag cccggagcaa cagacgtaac
    11041 aatcacttta aaaaacggag ataaattctc tttccataat ctctcaattc cggatttttc
    11101 tggtgcagca gcgagtggcg cagctaccgc aataagaggt tcttatagtg ttactgttgc
    11161 acataacaaa aagaaccctc aggccgcaga aactcaggtt tacgctcagt cttcttacaa
    11221 ggttgttgac agaagaaatt ccaatgattt tgagattcag aggttaaata aatttgttgt
    11281 ggaaacagta ggtgccaccc cggcagagac caaccctaca acatattctg atgcattaga
    11341 acgctacggt atagtcactt ctgacggttc aaaaaaaatc ataggttttc gtgctggctc
    11401 tggaggaaca tcatttatta atggtga
//
LOCUS       DBKELA010000117        11202 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.117, whole genome shotgun sequence.
ACCESSION   DBKELA010000117 DBKELA010000000
VERSION     DBKELA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.117"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..480
                     /locus_tag="ACUQR0_003385"
                     /pseudo
     CDS             1..480
                     /locus_tag="ACUQR0_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            582..2036
                     /gene="amn"
                     /locus_tag="ACUQR0_003386"
     CDS             582..2036
                     /gene="amn"
                     /locus_tag="ACUQR0_003386"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HHV9110288.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2379..3095
                     /locus_tag="ACUQR0_003387"
     CDS             2379..3095
                     /locus_tag="ACUQR0_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHV9110289.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3551..3626)
                     /locus_tag="ACUQR0_003388"
     tRNA            complement(3551..3626)
                     /locus_tag="ACUQR0_003388"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3591..3593),aa:Asn,seq:gtt)
     gene            complement(3727..5198)
                     /gene="yeeO"
                     /locus_tag="ACUQR0_003389"
                     /pseudo
     CDS             complement(3727..5198)
                     /gene="yeeO"
                     /locus_tag="ACUQR0_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5359..5434
                     /locus_tag="ACUQR0_003390"
     tRNA            5359..5434
                     /locus_tag="ACUQR0_003390"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5392..5394,aa:Asn,seq:gtt)
     gene            complement(5472..6422)
                     /gene="cbl"
                     /locus_tag="ACUQR0_003391"
     CDS             complement(5472..6422)
                     /gene="cbl"
                     /locus_tag="ACUQR0_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HHV9110290.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6524..7441)
                     /gene="nac"
                     /locus_tag="ACUQR0_003392"
     CDS             complement(6524..7441)
                     /gene="nac"
                     /locus_tag="ACUQR0_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HHV9110291.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7769..7844
                     /locus_tag="ACUQR0_003393"
     tRNA            7769..7844
                     /locus_tag="ACUQR0_003393"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7802..7804,aa:Asn,seq:gtt)
     gene            complement(7900..8835)
                     /gene="ldtA"
                     /locus_tag="ACUQR0_003394"
     CDS             complement(7900..8835)
                     /gene="ldtA"
                     /locus_tag="ACUQR0_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HHV9110292.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8897..9976)
                     /gene="cobT"
                     /locus_tag="ACUQR0_003395"
     CDS             complement(8897..9976)
                     /gene="cobT"
                     /locus_tag="ACUQR0_003395"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HHV9110293.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9988..10731)
                     /gene="cobS"
                     /locus_tag="ACUQR0_003396"
     CDS             complement(9988..10731)
                     /gene="cobS"
                     /locus_tag="ACUQR0_003396"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HHV9110294.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10728..>11202)
                     /gene="cobU"
                     /locus_tag="ACUQR0_003397"
     CDS             complement(10728..>11202)
                     /gene="cobU"
                     /locus_tag="ACUQR0_003397"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HHV9110295.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3119 a         2844 c         2427 g         2812 t
ORIGIN      
        1 catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc ttctggggaa
       61 cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa gccatctcag
      121 taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct cgtctgggga
      181 atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa aaaaagaata
      241 aatggtagtg tccgctattg ccagtacacc tcaatgtttg gtgccagtta tcgctacagt
      301 ggtgctggag tcggttatca ggttgccagt gtggttggcg gtggatttac accttttatt
      361 gccgctgcac tcatcactta ctttgccggg aactggcata gcatcgccat ttatttgctg
      421 gctggatgcc tgatttctgc aatgaccgct ttattgatga aagacaatca acgctcgtga
      481 tagcctggcg aagatcatcc gatcttcgcc ttatactttt gtttcacatt cctgtgacat
      541 actatcggat gtgcggtaat tgtatggaac aggagacaca catgaataat aagggctccg
      601 gtctgacccc agctcaggca ctggataaac tcgacgcgct gtatgagcaa tctgtagttg
      661 ctttacgcaa cgccattggc aaatatatta caattggcga attacctgat gaaaacgccc
      721 gtaaacaagg tctttttgtc tatccatcac tgaccgtaac ctgggacggt agcacaacaa
      781 atccccccaa aacgcgcgca tttggtcgtt ttactcacgc aggcagctac accaccacga
      841 ttactcgccc tactctcttt cgttcgtatc tgaatgagca actaacgttg ctttatcagg
      901 attatggtgc gcatatctct gtgcaaccct cgcaacatga aatcccttat ccttatgtca
      961 tcgatggctc tgaattgaca cttgatcgct caatgagcgc tgatttaact cgctacttcc
     1021 cgacaacaga actggcgcaa atcggcgatg aaactgcaga cggcatttat catccaacgg
     1081 aattctcccc gctatcgcat tttgatgcgc gccgcgtcga tttttccctc gcacggttgc
     1141 gccattatac cggtacgcca gtagaacatt ttcaaccgtt cgtcttgttt accaactaca
     1201 cacgttatgt ggatgaattc gttcgttggg gatgcagcca gatcctcgat cctgatagtc
     1261 cctacattgc cctttcttgt gctggcggga actggatcac tgccgaaacc gaagcgccag
     1321 aagaagccat ttccgacctt gcatggaaaa aacaccagat gccagcatgg catttaatta
     1381 ccgctgatgg tcagggtatt actctggtga atattggcgt gggaccgtcc aatgctaaaa
     1441 ccatctgcga tcatctggca gtgctacgcc cggatatctg gttgatgatt ggtcactgtg
     1501 gcggattacg tgaaagccag gccattggag attatgtcct tgcacacgct tatttacgcg
     1561 atgaccacgt tcttgatgcg gttctgccgc ccgatatacc tattccgagc attgctgaag
     1621 tgcaacgtgc gctttatgac gctaccaagt tagtcagcgg caggcctggt gaggaagtca
     1681 aacagcggct gcgtacaggt actgtggtaa ccacagatga caggaactgg gagttacgtt
     1741 actcagcttc tgcacttcgt tttaacttaa gccgggccgt agcaattgat atggaaagtg
     1801 caaccattgc cgcgcaagga tatcgtttcc gcgtgccata cgggacacta ctgtgtgttt
     1861 cagataaacc gttacatggt gagattaagc ttcccggcca ggctaaccgt ttttatgaag
     1921 gcgctatttc cgaacacctg caaattggca ttcgggcgat cgatttgctg cgcgcagaag
     1981 gcgaccgact gcattcacgt aaattacgaa cctttaatga gccgccgttc cgataataag
     2041 ttatgaacct tcttatcaga aatagatgtg aaggattaag taagacatct ggcaaatagc
     2101 ctgcaataaa cgcgggctgc ttgcttcctt gggcagatgc gagttttatt atcgtcttaa
     2161 tgatttccac atattaaaag caagtatgct ttcaaaacac aattataaaa aatcccgcca
     2221 acaacattag tttttataaa attaaatata agattatggc tttagaatat ttttatttct
     2281 aatagacgag atttttcctg ttatgatata atatgccgaa ttaacacatg ttaacgattt
     2341 accagtaatg taaataaatt ttcgaggaga tcattccagt gggacgtaaa tgggccaata
     2401 ttgttgctaa aaaaacggct aaagacggtg caacgtctaa aatttatgca aaattcggtg
     2461 tagaaatcta tgctgctgcc aaacaaggtg aacccgatcc agaattaaac acatctttaa
     2521 aattcgttat tgaacgtgca aagcaggcac aagttccaaa gcacgttatt gataaagcaa
     2581 ttgataaagc caaaggcggc ggagatgaaa cgttcgtaca gggacgttat gaaggctttg
     2641 gtcctaatgg ctcaatgatt attgctgaga cattgacttc aaatgttaac cgtacgattg
     2701 ctaacgttcg cacaattttc aataaaaaag gcggcaatat cggagcggca ggttctgtca
     2761 gctatatgtt tgacaatacg ggtgtgattg tatttaaagg aacagaccct gaccatattt
     2821 ttgaaatttt gcttgaagct gaagttgatg ttcgtgatgt aactgaagaa gaaggtaata
     2881 ttgttattta tactgaacct gctgaccttc ataaaggaat cgcggcgcta aaagcagctg
     2941 gaatcactga gttctcaaca acagaattag aaatgattgc tcaatctgaa gttgagcttt
     3001 ccccagaaga tttagaaatc tttgaagggc ttgttgatgc ccttgaagat gacgacgatg
     3061 tacaaaaagt ttatcataac gtcgcaaatc tctaattata ttttaaagaa atctgtcttt
     3121 atggcagatt tctttaatct catataattc ttataaaaaa tataatattt aactcgtcat
     3181 attgcttata aaaaatcccc gtccccagag aaataatatt tattaaaatg gcaggtcttc
     3241 tttttctgat taccgaagac aaagtggcaa ttacgtatag tttcccgata aagacgcgac
     3301 agctacgtcc cgcataaggc atttttctct ttatctttgt acggtacttc atggaacaga
     3361 atttttgacc ttgtgaatcg tgatatctgt tggggagggg caatttgctc actgaagcgt
     3421 gagactcgat taagcgcacg aaacacagaa atcaaaaaac ccggccactt ttttacaaag
     3481 tggccgggta aaaataattt ttatttttta actgtcctga gtctcataga gatgtctcaa
     3541 aactaaaatt tggctcctct gactggactc gaaccagtga catacggatt aacagtccgc
     3601 cgttctaccg actgaactac agaggaatcg tgagaacgag gcgaatatta gcgatgccca
     3661 cccacaatgt caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata atctgcattt
     3721 tgtcgtttat tccgacacaa ctggcttttt ttcacacttt tgcggctcgg atcgagggta
     3781 tttccacagc caacgtccag taaccattcg ccagtaaaac agcacggccc gcacagccca
     3841 gtcggcaaac attcccatcc agacaccaac cacaccccag ccaagcatga ttccctgcac
     3901 ataaccgact acaacccggc aaccccacat gctcaacata gaaacccaca tggcgtaacg
     3961 ggcatcacga gcacccttaa atccagcggg tagcacccat gaggcggacc aaataggcat
     4021 aaataaagca tttagctaaa tcagaatcac aacgacgtgt ttaacctgtg gatcctgggt
     4081 gtaaaacgat gccataaccc cggcaaaggg agccgttagc caggcgatgg ccgttaatcc
     4141 aagagtggac agccagaaca catgccgcaa ctgaatctct gcttgcgcta tctgccctac
     4201 ccccaacctt cggcctgtaa tgatcgtaga agcagaaccg agcgcacttc cgggtaagtt
     4261 gataagagcc gcaattgaga acgcgataaa atttccggca ataacgctgg tccccatccc
     4321 ggcaacgaac atttgggtta ataaccgacc actggtaaat aacactgatt cgacactcgc
     4381 tggaatacca atccccatga cttcccagat aatgctaaaa ttcagcggtt taaaatagct
     4441 ctttaacgaa atccttagcg caggattaaa accaatcgcc agcacccaca aaattgcaac
     4501 tgcgccaata taacgagaaa tggttaaacc caacccagcc ccgaccagga gaaaaggccg
     4561 taaatcaata tgccgctaat aataatatta agaatattca ggctaccgtt aatcagtagc
     4621 ggtattttcg tattccctgc accacgaagt gccccgctac caataagagt gatggcagcg
     4681 gctggataac tgagtacggt cagctccaga taagtcaacg ccagtgcttt aacttctgtc
     4741 gtggcatcac ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat tagcgttgcc
     4801 aacagtacgg caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt
     4861 cgtcgatccc gcttaccgag actaaatgct acaacgacag tagtaccaag atcgatagct
     4921 gcaaaaaaag ccataatgac catattgaag ctgtccgcca accccacgcc ggccatcgca
     4981 tcttttccca gccaactgac cagaaaagtg ctcaggcccc ccatcaacag gacacaggcg
     5041 ttctccatga agataggaac agcaagcggg gttatctcgc gctagaacaa cactttgtag
     5101 ctcttgcgtt tagagtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata
     5161 ttcaaagccg accttaattg cagaaagtga aaccacattt caaatgatga gggagaatca
     5221 gcaaagctgc aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga
     5281 aacggcaaac agttgggaaa tttaaaaatc gggtttgaca aaatattttt cgccgttaag
     5341 atgtgcctca acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg
     5401 tatgtcactg gttcgagtcc agtcagagga gccaaatttc tgaaaagccc gcttttatag
     5461 cgggcttttt gctatatctg ataatcaatt tcctcttcac tgttttccat cacctggcgc
     5521 ttgatatcct caactgacag tcctgcatta caaaattcca gaaagcgcca aacatagtta
     5581 cgctgaagtt gtcctcgctt cagtcccaac cagacagtat tagcatcgaa aagatgccgc
     5641 gtatccaggc ggattaaatt ctcttcctct tgttcgccgc tggattgttc ggcaactaat
     5701 ccgatcccaa gcccaagagc aacataggtt ttaatgacat cagaatcctg tgcacttaat
     5761 acaatatctg ccggcaaacc tttgcgggta aatgcgtcat cagtgcgtga gcgccccgta
     5821 attccctgtc ggtaagtaat taacggccac ttcgctattg attccagcgt caatggtgta
     5881 atttgcgtta agggatgatc gagtggaaca agcaaactat ggtgccaaca aaaccacggg
     5941 aaggcgacga gctgcgggtc attactcaaa cgctcgctgg cgataccaat atcagcttcg
     6001 ccattttgca acaacgtcgc aatttcctgt ggcgaaccct ggattagctc gagccgaacc
     6061 tccgggaaaa gttcgcgaaa agctttaatg acctctggca agctataacg tgcctgagta
     6121 tgcgtcgttg caatagtgag aacaccagac gtatcgttgg taaacaggtc tgcaagccga
     6181 cgaacattac tggcttcatt cagaatacgt tctgcaatga ccagtaatgc tttgcccggt
     6241 tcagtcatcc ccagcagtcg cttacctcgt cgaacaaata tttcgatgcc aaattcatcc
     6301 tccagttccc gaatatgacg gctaacgcct gactgtgagg taaaaagcat attcgcaacc
     6361 tctgtcaggt tgtaatcctg acgtgcagcc tcgcggatta tctttagttg ttggaaattc
     6421 acggtaaact ccgggcagtt cagatatccc gttattgtta aagtctaatg cccggcataa
     6481 caaataataa aaacccgcat cttattccat cccgatataa cgcttagctc accaattgcc
     6541 actgcctttt ttccatcact ggagaactaa tcactgacat taacaactct ttcactgcct
     6601 gtgcctgtgg tgacaagttt gctctggcgg gtaaatttaa ggacaaagag agactcattg
     6661 aaggagtggt aatgcgtgac atccacccat ttactgcgcc acataacgaa cgcgcagccg
     6721 attcaggtaa tactgcaacg cccatgccgc tggcaatcgc tgcggtaagc gtggcaatag
     6781 actcaatttc accaatgact tttgccgtga gccgccgtag ggaaaaagcc tcatcaacac
     6841 gaagtctaac agcgctgtaa tcactgggga gaaaaaggtt catttgcgca atagcattaa
     6901 catcaacgtt ttgccccggg caatcttgag ttcctaccag gaaaagatct tctttcagca
     6961 aagcctgact ggatacccca gccacagggg aatgctcata aatcaccgcc atatcgagtt
     7021 ggtgatttat cagtttttcg ttaagcactg caccactatt ttcatgaaga tagataacga
     7081 cctccgggaa ttcagcgcga accgcctgta ataagggcat ggtgatggat gacgcagcgg
     7141 ttcctggcgc aaagccaatc gagacttgcc ccgataatga ctgaccaacg ttatgcaccg
     7201 ccagttgggc ctgctcacac tgacgtaaaa tggcccgtgc atgggtatag ataatttttc
     7261 cggcgtctgt tggcgtaacg ccccgttttg tgcgaatcaa aagctgttga tttaactcac
     7321 cttccagtat ggcaacctgc tggctgagcg ctggttgtgc gatatgcaat acttcagcag
     7381 cctgggtcag gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca
     7441 tgttgcctcc ggtttttaag aatcggccca agtgccgcca ttacttacaa ccagtatgca
     7501 agatgcctgc cagttctatt ttggcgttga tgcgcaagct aaccaactgt caaataagag
     7561 attatgatag attcgtcatt ggcccttttt atcagcagtc gcattcacct gccctataaa
     7621 aggagggtat gcaccacgat ggttcattac ccaataagat tgaaagctca ccactttgtt
     7681 gaaattggca gcaaacaaac aaaaaaaatg catttcaccc tttgacatca ccatgcactg
     7741 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     7801 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
     7861 ctttcgagca ggctttttac tttctaatta ccaacgctct taaaacatct gtcttgaacc
     7921 agaactaatt tgcacaggca ttcccgatcg atgttgcaac gcagcatttg cgcgatttac
     7981 atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt
     8041 tcggtcagac tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccactt
     8101 tgaaccatcc ggttccgttg tgtatttcac tggctgatca ataatttgta cacgagtgcc
     8161 tacaggaaca ttatcaaaca gatatttgat atcgtcatta cgcagacgaa tacagccctg
     8221 acttacacgg agcccaatac caaaattggc attggtaccg tggatggcat acaacctgcc
     8281 aatataaatc gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa atgcgggcaa
     8341 actctcccct cgtttcgcat attcgcgccg ggtgttcggc gttggcgtcc aggttggtgc
     8401 ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc
     8461 gataccaata ggaaagactt ccactgtatt actgtctggc ggatagtaat aaagacgcat
     8521 ttcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg
     8581 aatggtgagt tgtgagccag gcttcggcaa aaaaacatca gcgcccggat tcgcttccag
     8641 catattactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gagtattgtg
     8701 atcaggaaca gttacagtaa gcgactgccc cactaaacgg ctaccctctg gaggtaatgg
     8761 ataagttacc gccaggctat tttggctggc aaaaagcaga gcaaatgagc aaagaatatt
     8821 tacacgacgc atcatgtccc tttcctaagt cgcgaatact atccgataag tatagctttt
     8881 atcagacttt tcgtttttag ctgttcaaat cagaagtcgt attccccggt agaacaatat
     8941 tactggcagc aagttcgccc atgttgttgt atatcgcaca agcagcttcg atgatggaca
     9001 tcgccagagc tgcaccactc ccctcaccta aacgcatatc catattgaga taaggctcca
     9061 gccccaaatg cgagagcgct atacgcgcgc ctttttctgc cgacaagtga gaaggaatga
     9121 gatacggttt gattgcagga gacatctggc aggctgcgag cgcagcagca taagaaagaa
     9181 atccatccag caacacgggt aaaccgcagg aagcagcacc taacatcact ccagctattc
     9241 cgaccaaatc aaatccaccc acttttgcca ggacatcaac accatcctga ggatttggtt
     9301 gattcaacgt aatcgcccga cgcacaacat caattttatt agccagttta tctgtcggca
     9361 ggtttgcgcc aatcccaacc acttcttcag gatcccggcc agtgattgtg ctgactattg
     9421 ccgctgccgg tgtcgtgttt gccatcccca gttcacctac accaaacagc gtgacaccgt
     9481 ttttcgccag ctcccgcgta taacatatga cgtccaaaag caacttttca gcctgacggc
     9541 gactcattgc cggagctgaa gcaatattgc cgctacctcg tgcgacacgc atgttgataa
     9601 gcccggggat aggctcagca gtatcaatac caacatcaat tacgtggacg ttagcgcccg
     9661 cttgtgctgc cagcacacac acgccggttg ttccacgggt catattttca gcctgtatgg
     9721 ctgtcacttc ttttggagaa atagcgaccc cttcctccca gacgccgtga tcggcacaca
     9781 taaccagtac cgcttttttg cccacatgcg gtatgccatt cacccccggc attcctgcca
     9841 gttgtatggc aagcgcctcc agctttccta ggctaccaac aggtttgaat aacccgtcaa
     9901 catgccgttg tgcacgcgac atagcggcag aattgatggc tgggatcgta ttcagtaaat
     9961 cggcaattat ttgcatgtca cgtttcttta tagcagtgcc agcaggaaga ccagttcacc
    10021 aagctctatg gctgctccca gcgtatcgcc cgtttgcccg cccagcgtac gtttgagaag
    10081 ctgaccgagg atgaaaatcg ccaccatcgt caccaccata gcagccacac catgcatacc
    10141 cagcaataat acggcagcaa aaatagcggc taagccgagt gtgacgcagg tttgtcgccc
    10201 atcaattttg ccaatgaaca cattgccaag cccttcttcc cgcgcataac gatgacgata
    10261 catcaataat gcggcagtcc cgcggctgac cgcacatgct gccgccagcg atgcaaggat
    10321 cggttcgcca cgcagagcca actcgcttaa caccagaatc tttgccagta ccacaaaaat
    10381 caatgccaga ccgccgtggg tgcctaaacg gctatcacgc atgatttcca acatgcgatc
    10441 gcgactacgt gcagaaaata cgccatcaca ggtatcagcg aggccgtcca gatggaattc
    10501 cccggtcatc agcgccagca caagtacgct aaacagtgcc gccagtggtg cgccacacca
    10561 tgcctgcagc accatgaaga ccagcccgct aatcgcgcca agcaataatc caatcaaagg
    10621 aaaagtaata ataccgcgag aataatgctc gaaatccagt ccctgggacc agcgacgcgg
    10681 tacaggcaga cgcgtaataa atgagagcat cgcccaaaat aatttgctca tttgattttt
    10741 actccaatac ccgaaaccac cagccatact tcatttgccg cagcggccaa ctgctgattt
    10801 acccgcccgg caatatcacg aaaatgtcgt gccagacgac tctccggcac aatccccatt
    10861 cccacttcgt tagtcactaa tacaaccttt gcggggcaac gttggcaggc agcaatcaac
    10921 gactgaatct cagcattaat cgcctgttcc atcgcctgat aatcccattc atcagggtct
    10981 ttatcgccgc cataatcaaa caacagatta gtcaccattg tggtaacgca ttccagcaac
    11041 acagcctcat tcgggttaat gtctgcatga attaattcat caagatgttg ccagcgctcc
    11101 actgtgcgcc agtgctccgg gcggctttgc cgatgatgtt ctatccgtgc agccatctca
    11161 tcatcaagga tttgcgaggt agcgatatac agaacctgtg aa
//
LOCUS       DBKELA010000118        10894 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.118, whole genome shotgun sequence.
ACCESSION   DBKELA010000118 DBKELA010000000
VERSION     DBKELA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.118"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="ACUQR0_003398"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="ACUQR0_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HHV9110296.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="ACUQR0_003399"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="ACUQR0_003399"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HHV9110297.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="ACUQR0_003400"
     CDS             complement(2271..3686)
                     /locus_tag="ACUQR0_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9110298.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="ACUQR0_003401"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="ACUQR0_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HHV9110299.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="ACUQR0_003402"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="ACUQR0_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10894)
                     /gene="mdtA"
                     /locus_tag="ACUQR0_003403"
     CDS             complement(9875..>10894)
                     /gene="mdtA"
                     /locus_tag="ACUQR0_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HHV9110300.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREYAK
                     KGARS"
BASE COUNT         2507 a         3070 c         3000 g         2317 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggt
//
LOCUS       DBKELA010000119        10595 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.119, whole genome shotgun sequence.
ACCESSION   DBKELA010000119 DBKELA010000000
VERSION     DBKELA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.119"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..337)
                     /locus_tag="ACUQR0_003404"
     CDS             complement(<1..337)
                     /locus_tag="ACUQR0_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagK/YfjJ domain-containing protein"
                     /protein_id="HHV9110301.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(365..927)
                     /locus_tag="ACUQR0_003405"
                     /pseudo
     CDS             complement(365..927)
                     /locus_tag="ACUQR0_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246234.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(991..1337)
                     /locus_tag="ACUQR0_003406"
                     /pseudo
     CDS             complement(991..1337)
                     /locus_tag="ACUQR0_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOTE conflict system archaeo-eukaryotic primase
                     domain-containing protein"
     gene            complement(1444..2007)
                     /locus_tag="ACUQR0_003407"
     CDS             complement(1444..2007)
                     /locus_tag="ACUQR0_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HHV9110302.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3382..5217)
                     /locus_tag="ACUQR0_003408"
     CDS             complement(3382..5217)
                     /locus_tag="ACUQR0_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110303.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5318..5605
                     /locus_tag="ACUQR0_003409"
     CDS             5318..5605
                     /locus_tag="ACUQR0_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9110304.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5577..7106
                     /locus_tag="ACUQR0_003410"
     CDS             5577..7106
                     /locus_tag="ACUQR0_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HHV9110305.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7276..8493)
                     /locus_tag="ACUQR0_003411"
     CDS             complement(7276..8493)
                     /locus_tag="ACUQR0_003411"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9110306.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8860..9846)
                     /gene="ycdU"
                     /locus_tag="ACUQR0_003412"
     CDS             complement(8860..9846)
                     /gene="ycdU"
                     /locus_tag="ACUQR0_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HHV9110307.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9843..10371)
                     /locus_tag="ACUQR0_003413"
                     /pseudo
     CDS             complement(9843..10371)
                     /locus_tag="ACUQR0_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            10438..>10595
                     /locus_tag="ACUQR0_003414"
     CDS             10438..>10595
                     /locus_tag="ACUQR0_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHV9110308.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSK"
BASE COUNT         3302 a         2432 c         1996 g         2865 t
ORIGIN      
        1 tacaccagac atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca
       61 cagcttccct actccagagg taatataaag gggtgggctc gactgattta tctttacgtc
      121 gcttacactg caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca
      181 gagaaggaat tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa
      241 ttttaggatg tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg
      301 cgttacagga aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa
      361 taggtcaaaa accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg
      421 tcaaataatc cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa
      481 tatccaggat gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt
      541 ccaaccctaa ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct
      601 catagtttcc cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca
      661 tttaaaggac tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg
      721 tttgccttgt tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg
      781 gaaacgagat atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc
      841 aatcacagga gggtaatgga gatccccccg tattgctgtt attctggaag tgtttttaat
      901 attcgtattg atagttctca gagatatatc acactctaga tattttaatt aagtatcagt
      961 tcgatatcgg catattgtac actacctcag gtagatgatg agatcagata cagggatcaa
     1021 ctgacggtga gcgcagtccc cacattttat ccagggctta ctgcatattc ccaacagcca
     1081 ttcgttagcc aggtagacga ataccctgat ttgccgctgg ttttgctttc ctatcgaagg
     1141 gcccatacat catcacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg
     1201 gataatacga aaacgcactg cacagggctc tgcggtttac gtcgccattc aatcccatga
     1261 gcttcaagta atgagaccag ccgggtattt tctgcccgca gtactgctag ttcatcgctg
     1321 tcagatttat aaaccatatg cgttatcact tgtacctgtc tcccggcggc accacgactg
     1381 aaattcgtgt gaaggcaaat ccacgcgata acttacgata gttcggacat aagcccccat
     1441 aattcagtta tgtcgtggtc gaccccggtt acttttttcg ggaagcctgc tacggcccag
     1501 aataataccg tgttctttct gatcctgttt agccagatag ctaagggcgt aacgtaagtc
     1561 gtctaccgca gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc
     1621 tttagcccgg cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc
     1681 cagttgcctg gatatctgat aagagttctt gtgacgttgt ccatccaggt agccgataaa
     1741 atgaatatga agccctttgt ctggtgtata ttccattacc cagtaatatc ccgccaacat
     1801 cgtctgggtt tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg
     1861 cccgaaactg ggcgtgtctt tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga
     1921 aaaacgttga aacataccat ccatgtgttc attcacgtct ttcagaatca tgaaatccat
     1981 tttgtaattg ggattggcat tatacatttt atagtcctta ctttataaaa ctcacaggaa
     2041 gagagaatta cgtaaatact gagagtagag aaacgccgta ccacggcatt aataaacgaa
     2101 gtaaaccagc agcacagaca acctgataaa actgttttgt attaccgcat tactgtgatg
     2161 aggtaatact tcagaataca gtgagactct cttaaactga gaaccagtac gaattacaat
     2221 ggaaaaacac acctcataga attgagtttc aggaagcacc actaaattct ttacaggaag
     2281 taatctgaca ttcacaaaac tcagtatcgg ctagctacag gcaacatcgc tattaatatt
     2341 gtcgaaccat taagtaaagc cgagagagac cacgggtatt taatcattag cataaaagaa
     2401 taatggtata cagaacatac ccacacacca taagaagatt gttccataca gaaaagatca
     2461 tatttaccta tacctataaa ctgtaacgga gtcataacac gctctaatag tgttccgata
     2521 cgtaaccagt gttaagtacg tacaggacaa cattacacac ataattcacc atacagcttc
     2581 ccccatatgc ttcactgcgt agtgtatgga gatatatgca ttacacacat acagaatact
     2641 tagcataaag aggagtgaca ttaccacgta aagagataag ttatacaaca aacatacaaa
     2701 caatacggca cagacacaca ataccataac tccaactaat aatactaatt aaccttaatt
     2761 atttatggta tatgaaataa atgtgattct cccggaacta actaacctaa ccagacagat
     2821 gatcactgta taaaaacttc agttaatact catttgtttt gtcgactcag tatataatga
     2881 gtgaccagta atcaggataa tcacacttac ataacctgta aaaaagaaca agaagaataa
     2941 gccactaaca taatgcaaaa tggcatctga atcgatcgca gtttggcatt cgaatcgatc
     3001 atgatttggc ccaatccgtg atcgaataga gcggaatacg caacataatt caccagacaa
     3061 gagcttacac cgaattacag attatgtatt aatgtcaaaa ctggcatatt ttacagatac
     3121 aatgaacatg caacccatca atctaatagg ggcggtatgt acatctgccc atgaatggga
     3181 tggcgctaag aatacaaaac aattagacat agatcacaaa aacacaccat tctaattgaa
     3241 aaacaaaaca aacaaataaa tcatactatt atagaatttt ctcttgccag acatagataa
     3301 tacccctgct aattttgcta atggttagca aaattagcag ggtatataaa gattattgac
     3361 ccaaaacaca ctcagatgaa ttcacgacag ctcaggaggg ttattcctga tgtcatcttc
     3421 acaaataatg gttaacgcat catatatcat tctgcgtctg ttcctgtttt cggccttatc
     3481 gccatacaac atcatcacat ttttaatttc atcgaccggt gatttcacat agtgaactaa
     3541 tacaccaagc ttctcaacac attgcagtaa agtatatttc ttactgcctg tgaaaagccc
     3601 tatcatagtt tctttacgtt cgcgactgga taatactttg ttttccataa tgacttctgc
     3661 cagcgtctta ccatcataaa catactcttt atctatcaga atatttatct tatccagcac
     3721 cttctctacg tcatcaagca aacctgtaat atatgggggc gcaatatatg gtttcgtact
     3781 gatcttttca agcatacaat tatacatctt aataacacgc atcagggtaa aattgttcgc
     3841 atcatactgt atttcaccct ccttatatac aggatcaata agtggtattt tttcaataaa
     3901 tgcatcctga taggctttta catggtcaag ccaataaaac aacgagccat actttatatt
     3961 ttttctattt actttaacct ccagtgctgt ccgaagcaac gataatgccc ccctgacata
     4021 tcctgaaggt aattcaccaa acaatccttt tctttccatg tcatcataat gctgcagcac
     4081 atcattaatt ttatttttcc ttatcagaca aatgtacacc gcaagtgttt cgtagcagct
     4141 ggtcacatga acagcctcat tctgcaactg cccaaaatag cttataatct ttttcagata
     4201 tgcgtcatca caaccattct tatcattaat attaaataca agatctttca gacgggaaat
     4261 atattcctga gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact
     4321 gttttgaaac ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct
     4381 ggtaaagtca taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc
     4441 tctcaccaac actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat
     4501 atcaagtaag acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac
     4561 tttctccagt tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt
     4621 gatagccatg atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt
     4681 tcctgtatat ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa
     4741 tcccagttca gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt
     4801 atcagcgtga tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata
     4861 aaaagcagag tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac
     4921 cccaaaagtg cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg
     4981 ataagaattc atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc
     5041 ctgactgaac accccttcag catcaataat acgactgatc tcattataac gttgatccca
     5101 gaatttttga tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccctc
     5161 cttaaacaac cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg
     5221 ctgtgctctc cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa
     5281 actgacacaa cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct
     5341 ttacacgcct tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag
     5401 atctactatt tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa
     5461 accaataagg cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct
     5521 gatgttacat acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga
     5581 tgacgacaaa actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg
     5641 cattacttga tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta
     5701 tgctgacggt catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc
     5761 aacgtaccag ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct
     5821 ctgcctgcgc aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat
     5881 atatcgacga taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa
     5941 tcctggagcg gagatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac
     6001 gagcagcact tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca
     6061 tcgtgaatga cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc
     6121 tgttgctgtc tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac
     6181 tgtacaacac attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt
     6241 tccggctgaa tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata
     6301 agttttttac tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat
     6361 tttgcgaacc cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg
     6421 tgacgcaaca atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact
     6481 ctctgagagc atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat
     6541 caaatgcagc atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac
     6601 agaatcctca tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca
     6661 ggatagcagc acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa
     6721 gtactaatag cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg
     6781 aatcccttcg ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta
     6841 tcacaacagc aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc
     6901 tcaggaaaaa atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa
     6961 ggattgatga taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa
     7021 aaaaactaaa tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac
     7081 tgaagtatca ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg
     7141 tattattcgt tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg
     7201 aactggcatt gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta
     7261 tgccagtcca tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata
     7321 atccgcccat gcctgcatca tcggtacacg ctgctccagt agatcagtac gatgatatgc
     7381 cgcctcaacc ttatttttca gcgtatgagc gagcgccctt tccgccagat cccgcgaata
     7441 cccctgttca ctacaccagt ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg
     7501 ccccggaatg tcactgacgg ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga
     7561 aaggatctgc tgctttctgg gtgaagggaa caccagttca tcatgcaggc cacgtatatt
     7621 ttccagaatg taaatagcct gccgggataa aggaacacga tgctgtagcc tagctttcat
     7681 tctttctgca ggtatagtcc atacccgctt atgaaaatca atttcagccc agcgcattcc
     7741 cctggcttcg cccgagcgag ttgctgtaag tatcaccatt aataacagtg cgcgggtaac
     7801 attataaggt tcatcactat acacactggt cgccacaaaa agcggtaact gcctccaggg
     7861 cattgcgggt tggtgttcat cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac
     7921 atcaacagga tttgccacac aaaaaccgtg cgcccatccc cactgcataa caacatgaat
     7981 gcgctgttta acccggcttg ccgtttctga caaggttaac cagactggac gcagtgtttc
     8041 tgccacgtcc gcagccgtaa tcgaatccag cgtttttgct cccagttgag gaaacgcgta
     8101 attctcaagc gtcgataacc actgccttac atgctttgga ttctcccatc caggagacag
     8161 ttctgcatgt acacgcctgg ctgcatcggc aaatgttggg ataacgactt tctcagattc
     8221 agccttttta atctccagag gatcatcacc tgcagcaagt tgctctcgca ttatccgggc
     8281 agtacgtgca gcttcagcaa tactgacctc tgggtaagtt cccaatccag cattacgtct
     8341 tttttgtgtc accggactta cataacgaaa aacccatttc ccccgcccct ttactgaaga
     8401 aggatgaagg gtcagtccgg taattccccc atggggcaat ggtttgtcat caggtttgat
     8461 atgtcttgct ttcgtatccg tcaatactgc catacgctaa ttcccgtcac tctggtatgc
     8521 catccagtat gccattagtc cctcgcttca ctaagaatac attagataac atcggacaac
     8581 aaaaagtgta atacattgtt tataaagaaa ttatataaca cccctggatt aagtcagatt
     8641 tatttcaggc ggtccccctc accgccatat ttaaagaaga gcgcgtacga aagtacgggc
     8701 ttttttttcg tatattgtac acaccgggtg cccccgccca cgtctgttag ggcgagggga
     8761 aattgtgcac atcaactgtc tggttactca gtcaacaatc aaaatgcgta agatagtaca
     8821 gaacatatat gcgttatctg gtcacttcct gtaacttcct cacttattaa aatgccccca
     8881 acggaaatac atgtcacatg ccatcataaa atcgttatgc cagaggaatc gacaatggaa
     8941 aaaatagtaa attaagcacg gtacatagca gagtaaaaaa taggcttcaa accaaatccg
     9001 ccattccaca agcgttgcca ataccgacac cagcgtaatc cacatcattt caataacccg
     9061 caatatgaat aaccgaagcc aaagcggctt ggcaaaaaag gtcgacaacg tattttcact
     9121 ggagaaaaac agatcgattt cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga
     9181 gaagcgcaga aaaaacagat taatcgccaa cactacgcca caaagaatga gcaatgcaat
     9241 tatcgattta aacgttaacc actgcccctc tgtcacaaaa gaatcactgt tacgcaacgg
     9301 gctgatagta agcaggttaa caaccgttga gatgcacacc gaaagcagca catagcgagt
     9361 cagactttta tattttactt ttttcgctac tgaaatagcg acctgcttga cataactggt
     9421 gcggatcatc cgaaacagca gaaagatgac caacggccca agtaacgaaa tcaggaacag
     9481 cgcatataac gagggtagaa gctggtatag cccccaactc attgcactca acatgattaa
     9541 cgacagaaat cctgccacca gaggttgtgt aagaaataat tctattttgt ggttctgctt
     9601 tttcttaaac cacgaaaaat cgatattctt cgtgacgcat ttataagccc agtacgcctt
     9661 gagatcaaaa ataaaattat acagcaccag ggccagaatc ccactaacta caccaatatc
     9721 ctctgatgga aaagtaaacc ctgcattttt ccaataaaag cctatcagaa taagtccaat
     9781 agcataaatg tatatcagat ttcgtttatc ctgaaagcga ataaaagtta aataatcagg
     9841 aatcatttga atacctttgc atcactaaat gctttacgac aatgcataac tcgttgctga
     9901 tccacgccag caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg
     9961 aaagctaccg gggttggcat attcagaata tgtatcgaga gattcagtcc atttcaccgt
    10021 atgagttaag tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc
    10081 tgttacgttc atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc
    10141 gcgcattgcc tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag
    10201 tttaaccggt tcgtatccag cggccatcag cagcgtggta aattcattca acgcagcctg
    10261 cgttccccca ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc
    10321 tgattccgta atcatcgtct gcagttgttc gcggttgatg cctttaaccg cctgatctta
    10381 cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt gacttacatg
    10441 acaaaaacag tatcaaccag taaaaaaccc cgtaaacagc attcgcctga atttcgcagt
    10501 gaagccctga agcttgctga acgcatcggt gttactgccg cagcccgtga actcagcctg
    10561 tatgaatcac agctctacaa ctggcgcagt aaaca
//
LOCUS       DBKELA010000120        10548 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.120, whole genome shotgun sequence.
ACCESSION   DBKELA010000120 DBKELA010000000
VERSION     DBKELA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.120"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..558
                     /locus_tag="ACUQR0_003415"
     CDS             <1..558
                     /locus_tag="ACUQR0_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHV9110309.1"
                     /translation="LTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQRAKE
                     ARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKT
                     LAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHY
                     ELYDKPEAVQEALAKVIPFFNTHLG"
     gene            576..1214
                     /locus_tag="ACUQR0_003416"
     CDS             576..1214
                     /locus_tag="ACUQR0_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9110310.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1505..2233
                     /gene="mpaA"
                     /locus_tag="ACUQR0_003417"
     CDS             1505..2233
                     /gene="mpaA"
                     /locus_tag="ACUQR0_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HHV9110311.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2208..3167)
                     /gene="ycjG"
                     /locus_tag="ACUQR0_003418"
     CDS             complement(2208..3167)
                     /gene="ycjG"
                     /locus_tag="ACUQR0_003418"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HHV9110312.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3286..3792
                     /gene="tpx"
                     /locus_tag="ACUQR0_003419"
     CDS             3286..3792
                     /gene="tpx"
                     /locus_tag="ACUQR0_003419"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HHV9110313.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3836..5377)
                     /gene="tyrR"
                     /locus_tag="ACUQR0_003420"
     CDS             complement(3836..5377)
                     /gene="tyrR"
                     /locus_tag="ACUQR0_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HHV9110314.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5525..6586)
                     /locus_tag="ACUQR0_003421"
     CDS             complement(5525..6586)
                     /locus_tag="ACUQR0_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HHV9110315.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6583..7980)
                     /locus_tag="ACUQR0_003422"
     CDS             complement(6583..7980)
                     /locus_tag="ACUQR0_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HHV9110316.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8136..9134
                     /locus_tag="ACUQR0_003423"
     CDS             8136..9134
                     /locus_tag="ACUQR0_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHV9110317.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9245..10117)
                     /gene="ompG"
                     /locus_tag="ACUQR0_003424"
                     /pseudo
     CDS             complement(9245..10117)
                     /gene="ompG"
                     /locus_tag="ACUQR0_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10190..10548)
                     /locus_tag="ACUQR0_003425"
                     /pseudo
     CDS             complement(10190..10548)
                     /locus_tag="ACUQR0_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
BASE COUNT         2659 a         2811 c         2494 g         2584 t
ORIGIN      
        1 ctgacagaaa aacgaattaa agctgtagtg agtattacgg gtgttaatat cgggcgtctt
       61 tttcgggaag gtttcagcaa ttatgatccg attggcgtat tgaatgcaat ggcgtcacaa
      121 cgagcaaaag aggctcgtgg tggagaatta cagatcaacg aattacttcc cgccagcctg
      181 gatgcagcaa aagcccatgg actgaccgaa cgcgatgttt atgaagcaac tgactactat
      241 aaaacaccac gcgggcaaca accgggaggt gcgacaaaaa tgttgttctc acatgcacag
      301 aaaacgctgg cctgggatgc cttcgcattt actgaagtgt tactgaccca gccggtgatg
      361 gtagtagttg gtgaaaaagt cggtgccttc ggtgcttatc gtgatggact ggaagtctat
      421 ggccgggcga tggtttcgca ggatcgtcag ttagtttcgt tacccgattt ctcacattat
      481 gaactatatg acaaaccgga agcagttcag gaggcgctgg caaaagttat tccttttttc
      541 aacacccatc tgggataagt ttcatgagaa atattatgaa aaatgtactg attcttggtg
      601 ctggcggtca gatagcccgc catgtgatta atcaactggc agataaacaa actattaaac
      661 aaactttatt tgcccgtcag ccagcaaaaa tacacaaacc ctatccaact aatagccaga
      721 ttattatggg ggatgtactg aatcatgcag cactgaagca ggccatgcag ggacaggata
      781 ttgtgtatgc caaccttacg ggggaagatt tagatattca ggcaaattca gtgatcgctg
      841 cgatgaaagc ctgtgatgtt aaacgtctga tctttgttct ttccctcggt atttatgatg
      901 aagttccagg aaaatttgtc gaatggaata atgcagttat tggtgaaccc ctaaaaccat
      961 tccgcagggc tgcggatgca attgaggctt ctggactgga atatacaatt cttcgccctg
     1021 cctggctgac tgatgaagat attattgatt atgagcttac ctctcgcaat gaaccattca
     1081 aaggtacgat cgtatcacgt aagagtgtcg cagcattaat tacagatatc atcgataaac
     1141 ctgagaaaca cattggtgag aatataggca tcaatcaacc cgggactgat ggcgataagc
     1201 cattcttcat gtaaatcaat tatatagata ccatcgtact gtactatgtg cagtacgatt
     1261 tattaaactt attaaggtga actgatcaat cagatgcgat taacatgtgt agaaccagta
     1321 agaataaaat acgccatgta tataacatta cgacaattct aatatctgca catcaatgaa
     1381 aatgcaaaca acttccggca aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa
     1441 ggatttgata atccgcaaaa tttgcacaac actgcctgta atacttcctg aaccagagac
     1501 gatcatgacc gtaacccgct cacgcgccga acgcggcgca tttccgcccg gaacagaaca
     1561 ttacggacgt tcattattgg gcgcgccgtt aatctggttt cctgcccctg cagccagccg
     1621 cgaaagtggt ttgattctgg ctggcactca cggtgatgaa aactcttcgg tcgtcacgct
     1681 ctcctgtgct ctgcggactt tgacaccttc tttgcgccgc catcatgtgg tgctgtgtgt
     1741 gaatcctgac ggctgccagc tggggttacg ggccaatgct aatggtgtgg atttaaacag
     1801 aaactttccg gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga acagcgccgc
     1861 tgaagaacgt gatgtcgtat tactgacagg tgacaaacct ggctccgaac ctgaaaccca
     1921 ggcgctgtgc cagcttatac accgcattca acctgcctgg gtggtctcat tccacgatcc
     1981 actggcttgt attgaagatc ccagacacag cgaattaggc gaatggctgg cccagtcgtt
     2041 tgaattgcct ctggtaacca gcgtcggtta tgaaacgcct ggctctttcg gcagttggtg
     2101 tgcagatctc aacttgcatt gcatcaccgc agagtttccg cccatctcct ccgacgaagc
     2161 cagcgaaaaa tatctctttg cgatggcaaa cttgctgcgc tggcatccta aagatgcaat
     2221 tcgcccgtcg tgaactgaag cgccggttcc acatctaccg ccagccaggt cggtccgtca
     2281 agatcggcga aactgacctg cggcaccagc ggtaaagcgg cgctaatggc acgagaggta
     2341 cacaacatgc agcccagcat cagactgaaa ccttgtgcac gcgcttcagt cgccagcgct
     2401 tccgtcagac caccggtttt atcgagctta atgttaacca tctcatagcg ccctttcagc
     2461 gccttcagat tgctacgagt atgacaactt tcatcagcac aaatcggcaa cggatgaata
     2521 aaattctcca gcgccgcatc gtcctgcgca ggaagcggtt gttcaagcat cgcaacgcct
     2581 aaatccgcca atagctggca acgcgccgcc aacccttctg cacgccagga ttcatttgca
     2641 tcaacgatca gcgtggcatc gggcacagcg gtgcgaattg ccaccatccg ctcactgata
     2701 agatggttat ccagcttcac tttcagtaat ttcgcgcctg cctgccagag tgttgatgca
     2761 ctattggcca tctgatcagg cgtaccgatg acaaccgtct gtgcagtgat aactgtctcg
     2821 ggaagcgtta tgccgatcaa atcagccagc gattgctgct gttttcgcgc cgcgagatcc
     2881 cacaatgcac aatccagcgc attacgtgct gcgccggcag ggagaatttt ttgcaactcc
     2941 tcccgtgtca gccctttctc taattgcggc acgacgctca taatttgcgc cattaccgag
     3001 gcatcacttt ctccataacg cggatacggc gtgcattcgc cggtgccttt aataccctct
     3061 tcttccagtt caaccaccac cacgcgcgct tcactgcgac ttccccgggc aatcacaaac
     3121 ggggtatgta agggccaggc ttcctcaaat accttaacga ttctcatctt tactccttgt
     3181 tgtcgcggtg gtctgcggat taagcgcaaa tagcgtttgc tgtgttattg acagttagca
     3241 taaactaggt gtgacgttaa ctatatgtaa acaggaaaga taatcatgtc acaaaccgtt
     3301 catttccagg gcaacccggt tacagtcgcc aattccatcc cgcaggcggg tagcaaagcg
     3361 cagactttta ctctcgtggc aaaagatctg tctgacgtca ccctcggtca gtttgcgggt
     3421 aaacgcaaag tgctgaacat tttcccgagt attgataccg gtgtttgcgc cgcatcagta
     3481 cgtaagttta accaactggc aactgagatc gacaacaccg ttgtgctgtg tatctctgcc
     3541 gatctgccgt tcgcccagtc tcgtttctgc ggcgcagaag gtctgaacaa cgttatcacc
     3601 ctctccactt tccgtaacgc tgaatttctg caagcctacg gtgtggcaat tgctgatggc
     3661 ccactgaaag gtctggcagc gcgtgccgtt gtggttattg acgaaaatga caatgtgatt
     3721 ttcagccagc tggtggatga aatcaccacc gagccggatt acgaagcagc tctggctgta
     3781 ctgaaagcat aagttaatat gcctgatggt gttgcaccat caggcatatt cgcgcttact
     3841 cttcgttctt cttctgactc agaccatatt cccgcaactt attggcaatc gcggtatgtg
     3901 aaacgccgag acgttttgcc agtttgcgcg tgctgggata attgcgataa agctgggtta
     3961 ataccgagcg ttcaaaacgg ctggtgattt cgtccagcga accttccatc gcatcttcgc
     4021 ccacggctac cgttgcggcg tcataatccg gcaacaaaat atcctgtgga cgcagctcat
     4081 aaccgtccag ttgtgtcagt gcgcgataga tagcgttctt taactgccgc acatttcccg
     4141 gccacgcata acgtgtaagt acagtattca ggtcagcggc cagtttcgga cgcggcacgc
     4201 cctgctcgtc ggcaaagcgg gcgacgaaca gctcagttaa cggcataatg tcctgcggac
     4261 agtcacgtag cggcggcaga ttgagcgtca acacgttcag acgataatag agatcttcac
     4321 ggaacacgcc tttttgcacc agttcgacca gattcttctg cgtagcgcaa atcacccgca
     4381 catcgacatg cacctcatgg tcttcgccaa cccgacggaa agtgccatca ttaagaaaac
     4441 gcagtaattt cgcctgcatc cgtggtgaca tttcccctat ttcatccaac agcaccgaac
     4501 caccgttcgc ctgctcaaag aaccctttct tcccttccgg agcatgacca aacagttcac
     4561 tctcgaccgc atcttctggt atagacgcgc aattcagcgc caggtaaggt ttgcccgctc
     4621 tggggcttgc ctgatggcag gcgtaggcaa aaagatcttt acctgtacct gtgtcacccg
     4681 taatcagcag cggcgcgctt agcatcgcca gtttctgcgc ctgttcgaca acgtgcttca
     4741 ttttcgggct gacggcgaca atttggctga aggcgctgac gtcctgggcg gcgacatttt
     4801 gcaactggcg gcccatacga atcgttgatc gcaacatcac caccgcaccg gtcaggacgt
     4861 gttgatcatt ttcatcctga agataaacag gcgtaatctc catcaggaaa ttctgcccat
     4921 taataacgac atgctcgtta tgcgaatctt gcggttcgct ttccagccaa cgtaaaaaat
     4981 taaagccgtt aatcaattgt gcggcggtat ggttgcgcag gcgatccaat ttttgcccaa
     5041 aaagctgaca gctcgccggg ttcgccatat ccactttgct tttcatatcg acagagagca
     5101 caggttcagg caacgcctcc agtaacgcgc tcaacgccag atgctcacgt tcggaaggca
     5161 tccacgggac agtacgcaca tcggtaacac ctgcaatacg gcgtatttcg gccatcagac
     5221 tgctgaaact ctcaaactcc agttcagcaa aattgaggta gattcgccca atgggatcaa
     5281 tctcaatacc gcgtaaatca atgcctctta gcacgagtag atcgagtaat tcgcgggtca
     5341 gaccgagtcg gtcttcacaa aagacttcca gacgcatggg aaccttcacc tgaaaaaaga
     5401 acaattaata tgatgatatg acactatttg gatagcagga aggtttctgt caacaatcat
     5461 tgacacaaag acggaaaaac gggcacttcg gcgtaaagat atccggtcag cacccgatat
     5521 tgcattattt ttcgctgggc gttttgcctt tttgcagagt ttctttcacc tgaccgataa
     5581 gctgacgacg gaaatcccca aggcgaggtt tgtcatcgtc aatccacggc agcgggcggc
     5641 aaagctccat agctttaatc ccgagtcgta ccgtcagaag tccagcacca attccctgag
     5701 ctgcgcgggt agacaaacga gcagcgagat cttgcgacat ccagtccatc cccacttcgc
     5761 gcaccagttc gctggctccg gcaaaagcga tattcagcaa taccagctta aacagacgca
     5821 aacggctgta ataccccagt tcaatgccat acagcgtggc aatgcgatta attaaacgca
     5881 gattgcgcca ggcgataaac gccatatcga ccaacgccag cgggctgacc gcaatcatca
     5941 acgttgattc cgccgccgaa tggctgattt cgcgccgcgc ctgggcatct aaaactggct
     6001 ggaccaaatg cgcatacagg ctgaccactt cacgatcgtt ctgcgtttca tggattgagg
     6061 cataccagcg ttgcagcgcc ggatgcgact gatcaatacc cgcctgctgc gccagttttt
     6121 cgcaaaatgc gcgacctttg cccgtgccgt ggctatgcaa taaatcacgc gcttcgtcgc
     6181 gttcatgggc gcgctgtcgc aagcgccata agcgccgcca ctctgttacc acagaaccta
     6241 cgccagcgcc gataatcaat gccccagcgg cacatccacc cagcgctacc cagtcctggg
     6301 tttgccaggc attcattgtc cactgtatac cctgcccgac aacgcttgcg ccaaacagag
     6361 ccagcccgcc catcaccatt ttgcgccaca ggctgcgttt cggacgtaac gctgcgtcca
     6421 ttaccgcttc gacttgcccc tcttcttcct gcgcttcgtc gagcgtggcc ggggcaaaat
     6481 tttgcgcctg attttcgtca aaggtctgct gcgccctgaa ttttggattc tgttcgacct
     6541 ccagcggacc gtcgaaatca atacgtggtt ttaacggttc ggtcatcgca atttatctcc
     6601 tattaaaaat tccagcgcgg catcaagacg aatatgcggc agcggtttgt cgacatccat
     6661 cacctgcggg cgaaacgctt caaactggaa cccttgcttg tcccagaacg cctgaccggg
     6721 caaacgcgca ggaacttcac cggggtaaac ggtgagaggc gcaccatcgc tgagccggtt
     6781 accgcgcaat gccggaattt tctcgccgtt gacgtcaata atgccgctgg tggtcgcctg
     6841 caccgaggcc agccccaggc aatccatact aatcctttca aacgccgcat tttgccaggc
     6901 atcctgaatc agttgttgca gtaatgaaac catattggcg tgctgatcga ccgttacatg
     6961 gtccgctttc atcgcggcaa acagcagttt gtcgataacc ggcgagaata agcggcgaaa
     7021 cagcgtgcgc tgcccgtagt ggaaactttg catcagctgc gtcagtgcca gacgcatatc
     7081 attaaatgcc tgtggcccac tgttgagagg ttgcaggcaa tccaccagca caatctggcg
     7141 gtcaaagcgc agaaaatgat tcttatagaa ccccttcacc actttctcgc agtaataatt
     7201 aaaccgctcg cgcagcattc cggcattggt gtgcttatcg gcctgcgcca gtttggactc
     7261 gtcccaggca tcgacatccg gccacgggaa gaattgcagc gcgggcgcac ctgccatatc
     7321 tcccggcaag acaaagcgcc caggctgaat aaagtgcagc ccctgctgtt tacagtggtg
     7381 gagataatcg gtccacacgg cggcaatgtc tgccagccgg ttttcgtcgg caggcgctag
     7441 cgggtcaagt ccttcgcaca tcattcgcca tttcgccgac cattcgccgc gctgaccatt
     7501 gagtaagccc gtcatctggc gcgaccagct taagtaatcc tgcgccagca tcggcaggtc
     7561 gagcaaccat tcgccgggat aatccacaat ttccagatac agcgtggagg tatccttaaa
     7621 gtggcgcagc agcgaatcgt tcgatttaaa acgtaacgcc aggcgaattt cactgacgcc
     7681 gcgcgtaggc gtcggccagg cgggaggatc accatataac tgcgccagtc cttcatcata
     7741 ggtgaagcgc ggaatgccaa agtcacgctg gggaatgcgt tttacgccca gcaagcgctc
     7801 ttcacgcacc gcgcttaaca gcggcaaacg tgctccggca tgaatattga gcaactgatt
     7861 aaccatcgcg gtgataaacg ctgttttgcc gctgcggcta agtccggtta cagcgaggcg
     7921 cagatgtctg tcgacacccc gattcaccag cgcattgagt tcatttttaa gtcgcttcat
     7981 tgctgtcctg tgctgctctg aagaccagaa aaaaaatcat tcgtaaggat atgaatgtgg
     8041 gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac tccataagat
     8101 gtgcagcatt cacgttcgga cggagttcct gacctatgtc ccctactatt tatgatattg
     8161 ccagggttgc aggcgtatca aaatccaccg tatcacgcgt gctgaataag caaactaata
     8221 tctccccgga agcgcgcgaa aaagtgttac gggccattga agaattacag tatcagccaa
     8281 acaagctggc ccgcgcgctg acctcttcgg gttttgatgc cattatggtg atttctaccc
     8341 gttcgaccaa aactacggcg ggtaatccgt ttttctctga agttttgcat gccatcaccg
     8401 ccaaagcgga agaagaaggt ttcgacgtga tattacaaac gtcgcacaat ccggcagaag
     8461 atctgcaaaa atgcgaaagc aaaattaaac agaaaatgat taaaggcatt attatgctca
     8521 gttcgccagc ggataagtca ttttttgccc aactcgataa atacgatatt ccggtggtgg
     8581 taattggcaa agttgaaggt caatatgccc atgtttattc tgtcgatacc gataattttg
     8641 gtgacagcat tgcgttgacc gatgcattaa ttgaaagtgg gcatcaaaat attgcctgcc
     8701 tgcatgctcc gcttgatgtc catgtttcgg ttgatcgcgt taatggctat aagcaaagtc
     8761 tgtccgcgca taatattgca gtgcgtgata aatggattgt tgacggcggt tatacccatg
     8821 aaacagcact gcaagcagca cggcaattat taagtcagtc accgttgcct gaggcagtgt
     8881 ttgccactga cagcctgaaa ttaatgagca tttatcgtgc ggcggcagag aaaaatattg
     8941 ctattccgca gcagttagcg gtggtgggtt atagtaatga aacgctgtcg tttattttaa
     9001 cgcctgcacc aggcggcatc gatgttccga cgcaggagtt aggtcaacaa agctgcgagt
     9061 tattattccg cttaatttcg ggaaaaccgt caccacagaa tattaccgtt gccacgcata
     9121 tgacgctgaa ataaatacca gctaccttgc ctgaatgccg gatgcgatgc tggcgcatct
     9181 tatccggcct acatttatag atcggataaa gtactcgcgc cgcatccggc aatttagccc
     9241 attatcagaa cgagtaattt acgccgacac ctgcataatg gaatttatcg ctgtcgcctt
     9301 cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac ggaaagtccg ttctggaaat
     9361 cataaccgta aaataaaccc acacggttaa aatcatggcc ttcacgttca atatcatcct
     9421 gccagtccca gttactctag cgatccagcc caatgcgcgt atacggcgtc accgagtggt
     9481 tgccgagcgt cagcggcaaa taggcgcgaa tttcttgtgt ggaaaactca ccgttattgc
     9541 ggctgtcgtc catattgaag ccgcgctcga gataatagtt cactcgcaaa gcaaccgttt
     9601 cgttgaaggt atattgcaga cctgtttccg tttcgacacg ggtatcagcg taaccggtcg
     9661 tgttcaaatc gttggcaaat ttatacatcg acaaccaacc gttgaaacgt aaatcatcag
     9721 tcagtttcac atcccagtct ggcgcgattt tccagcgctg catattcgcc gtgtctttgc
     9781 ccggttcatc aacgtagtga taaccataat tacggaaacc gccggtcagg ccgaaactga
     9841 aatcatcgct ttcgaggaac tgataatgca cctccagctc cgggcgatca aacctcgttc
     9901 cacgtttacc cgcgctataa tctaccggcc cttcctgata ataggccaga gcaattctcc
     9961 acggcccgtt ggcggcatta aaatagactg aaggctccgc cagcccatcc atatcttcgc
    10021 cataaccctc gacgttttct atttcgtaca tcgcaccgat attaaagtgc cagtcgctct
    10081 tttcctcggc ctgtgcgcag gccattcccg cacacatcac cagtgcggta cagggtaata
    10141 actttttttg ttccttgtat tatcctgggg gaatgccttc cccctgtatt tagcgaattg
    10201 ctatttccgt ttctgcatca aagaaatgac atttcgtcat atcaaaatga atagtgatat
    10261 tttctcctgc atgataatca tttaacgcac cagcacggac cactaactcg tgcccccaac
    10321 cgtggtgtag agcataaatt ccgcaccggt taattctgcc acgctaattt tggcggaaat
    10381 gttattttcc tcttgcgcgt ccggatgaat atcttccggg cgtattccca tcacgatggg
    10441 cttatgcaaa ctttcctgtg ttttcagaac cgctaatttc tcttcgagaa tggttaattt
    10501 aagcgtttcc gtaacgaatt tatcgccatc gatcgtgccg cgaataaa
//
LOCUS       DBKELA010000121        10460 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.121, whole genome shotgun sequence.
ACCESSION   DBKELA010000121 DBKELA010000000
VERSION     DBKELA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.121"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..561
                     /locus_tag="ACUQR0_003426"
     CDS             <1..561
                     /locus_tag="ACUQR0_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9110318.1"
                     /translation="LNSLDTRMVPQASAWAINRVAQKAVSVATRQVAGNTVAGDNQVK
                     GIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRG
                     AFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPMSGPLTQAFEDAR
                     DRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            558..959
                     /locus_tag="ACUQR0_003427"
     CDS             558..959
                     /locus_tag="ACUQR0_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HHV9110319.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            970..1710
                     /locus_tag="ACUQR0_003428"
     CDS             970..1710
                     /locus_tag="ACUQR0_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HHV9110320.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1769..2155
                     /gene="gpG"
                     /locus_tag="ACUQR0_003429"
     CDS             1769..2155
                     /gene="gpG"
                     /locus_tag="ACUQR0_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone G"
                     /protein_id="HHV9110321.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2164..2493
                     /locus_tag="ACUQR0_003430"
     CDS             2164..2493
                     /locus_tag="ACUQR0_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HHV9110322.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2465..5203
                     /locus_tag="ACUQR0_003431"
     CDS             2465..5203
                     /locus_tag="ACUQR0_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HHV9110323.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5203..5532
                     /locus_tag="ACUQR0_003432"
     CDS             5203..5532
                     /locus_tag="ACUQR0_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9110324.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5532..6230
                     /locus_tag="ACUQR0_003433"
     CDS             5532..6230
                     /locus_tag="ACUQR0_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HHV9110325.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6235..6978
                     /locus_tag="ACUQR0_003434"
     CDS             6235..6978
                     /locus_tag="ACUQR0_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HHV9110326.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6942..7517
                     /locus_tag="ACUQR0_003435"
     CDS             6942..7517
                     /locus_tag="ACUQR0_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HHV9110327.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7578..10460
                     /gene="gpJ"
                     /locus_tag="ACUQR0_003436"
                     /pseudo
     CDS             7578..10460
                     /gene="gpJ"
                     /locus_tag="ACUQR0_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage attachment tail tip protein J"
BASE COUNT         2454 a         2485 c         3374 g         2147 t
ORIGIN      
        1 ctgaacagcc ttgatacccg tatggtgcca caggccagcg catgggcgat aaaccgtgtg
       61 gcacagaaag cggtctcggt tgccacccgg caggttgccg ggaataccgt tgcgggcgat
      121 aaccaggtga aagggatccc cctgaaactg gtacgtcagc gtgtccgggt gtttaaagcc
      181 agtccgtcag gaaaaatgac ggccaggatc cgcgttaacc ggggcaatct gcccgccatc
      241 aaactgaaca caacacggcg gcgtgctggt gaaggactga gagtgggaaa atactttttc
      301 cggggggcat ttgttcagca actggcgaat ggccgctggc atgttctgag gtgtcttcct
      361 gaagcgcgtt ttgcaacagg gcatgaccat cagggcaggc taagaaaaaa tcgtcttcct
      421 gtggaggtgg tgaaaatccc gatgtccgga ccgctgacac aggcatttga agatgcccgc
      481 gaccgcatca ttgctgcgga aatgccgaaa cagctggggt atgccctgaa acaacaactg
      541 aggttacatc tgagtaaatg aaccgacaca cacaaatccg tcagagcgtg ctggcacgcc
      601 ttcgggaaca gtgtggagac agcgccacgt tttttgacgg gcttccggca tttattgatg
      661 cgcaggaact gcctgccgtg gcggtgtggc tgagtgatgc tcagtacacc ggaaaaatga
      721 cggatgaaga tgactggcag gctgttctgc atattgccgt cttcatccgg gcacaggcac
      781 cggattcaga gctggatatg tggatggaga gcaccatttt cccggccctg aatgatgtac
      841 cggcactttc cggactcatc gacaccctga acccactcgg ttttaactat caacgtgata
      901 atgagatggc tacctgggcg atggcggaaa tcacgtacca gatcacgtac acgaattaag
      961 gaggtggtaa tgaccacacc aaatccactg gcaaagacga aaggtgcggg gacgacgttc
     1021 tggatgtata ccggcaacgg cgatgcgttt gcgaaccctt tgtcggacac tgactggctg
     1081 cgtcttgcga tggtgaagga tctgcaacct ggcgaaatga ccgctgatgc agaagatgac
     1141 acttatctcg atgatgaaga tgcagactgg aaaacgacaa cccaggggca gaaatccgtt
     1201 ggtgatactt cggcgacgct ggcctggcgt ccgggtgaca gcgggcagaa aaaactggtt
     1261 cagttgttcg actccggtga agtctgcgcg tttcgtatca aatatcccaa cggcactgtt
     1321 gatgttttcc gcggctggct gagctcactg ggtaaaacca ttgcctcaaa agacgtgatg
     1381 acccgcactg tgaaaatcag cggtgtgggg cgtccgtatc tggcagagga aggcactgaa
     1441 accgtgggtg ttaccggact gacggtggca ccggcatctg tcagtgtcaa agcgggagca
     1501 accaccacac tgacctttac agtaaaacct gacggtgcca gtgacaaagc gatcagtgtg
     1561 cattcgtcag atcctcagac tgcttcggtg accctgagcg ggcttgtggc cacggtgaaa
     1621 ggcgtgaagc agggcagtgt cagcatcgtg ggcatgacct ctgacggaga gtttgtggca
     1681 gtggctgcgg tgaccgtcag cgcaccataa caggacgata ctcatcattg ccccggttat
     1741 ccggggcttt ttgcatccgg agaacatgat gtttctgaaa caggatacgt ttaattatga
     1801 aaaacagtcc gtggtgctca gtgagctgtc cgggctgcag agaattgaat atctgacgtt
     1861 tgttcagcag cgaacggcaa agtttgatgc acaggaggga gaactgccgg aggctgaacg
     1921 acagattgct tttctgcgta tgggaatgga tatcaatgcc tggctggttt cccgctcact
     1981 gtggaatgct gagcagtctc aggatgttga gacgctttgc gcatccatta tgacaacatg
     2041 gtcgtatgat gcgctgggcg cgggggcgga gagggttctg tcgctgagcg gtatggggac
     2101 cattgagaat gccggggatg atgatcatga ggcgctgacg ccggaaaagt cctgacgcgg
     2161 gaaatgcagt ttgtcatgcg gcttgcccgg gagttccggc gggcagactg gcggcggatg
     2221 ctgtcggata tgtcggccac tgagcttggt gagtggggcg attatttccg gatgcagagc
     2281 ttcagtgatg tgtggatgga tgcgcagttt gcctcgctga aggcattgat cgtgagaatg
     2341 gtgtccggca gcagcgatgc tgcggtggct gatttcagcc ttttaccgga agagaacggg
     2401 ataccggagc gaacggacga agaactgatg catcttgggg aaggtatttc cggaggtgtg
     2461 cgttatggac cagatagcca acctggtcat tgatttgggg attgatgcgg cagagtttaa
     2521 aaatgaaatt ccccgtatca aaaaccttct gaatggtgca gccagcgatg cagaacggtc
     2581 ttctgcccgt atgcagcgtt ttatggagcg tcagactcag gcggcccggc agacaatgca
     2641 ggcggcttct tcggctgcaa cagcagcatc agcccatgcg cagacggtgg agaagaatgc
     2701 acgggctcat gaacgcatgg cccgtgaggt ggaacaaacc cgtctgcgcg tggatgccct
     2761 gaatcagaaa atgcgcgagg aacaggcgca ggccagggcg gctcagctgg gggtaagcag
     2821 tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga aaagccacac attcgctggg
     2881 gctgaaaagt gcggcagccc ggcgggagct gggggtgtta atcagtcaga tggcgcgcgg
     2941 caattttggt gcgctgaggg gatccgggat aacgctggct aaccgtgccg gatggacagg
     3001 cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt attggcggac ttgtcgcggc
     3061 ggtcctgggt ctgggtaaag cctggcatga cggccggaag gagggcgaga aatttaaccg
     3121 tcagctggcg ctgacgggac attatgccgg tgtcactgtc gggcagttgt ggaaactcag
     3181 ccgggccata tccgggaatg gtatcacgca acatgcggca gccggtgcgc tggcacaggt
     3241 ggtgggcagt ggggcatttc atggaaacga tattggtatg gtggcgaaag ctgccgcaca
     3301 gatggagcga tcggttggcc agtcggtcag cgataccata aatcagttta agcggctgaa
     3361 ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg ctgcattttt tgaatgccac
     3421 tcagcttgag cagatacgcg tccttgggga gcaggggcgg tccagtgatg cggcccggat
     3481 cgccatgtct gcgctggcag aggaaaccgg taaacgcaca tctgatattg ataataatct
     3541 caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg tggaagcagt tctgggatgc
     3601 ggccatgaat attggtcgtg aagactcgct ggatgcgcag attgatgcgt tacaggaaaa
     3661 aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc acacaggtgg agtacgatca
     3721 gcagcgtctt aacgatcttc aggagaaaaa acgccggaag gatttgcagg atgcaaaagc
     3781 gcaggcagaa cggaattacc aggagcaaca gaaacgccgg aatgctgaaa atgccgcgct
     3841 gaaccggatg aatgaaacgg aagctgcacg acatcagcgg gaaattgcgc gtattaatgc
     3901 catgcagtac gccgaccagg ctgtcaggga tgcggcgata cagcgtgaaa atgaacgtta
     3961 cgaaaaagcc attaagaaaa atacacgggc aacccgtaat gatgaggcca cccggttatt
     4021 gctgcagtac agtcagcagc aggcacaggt ggaagggcag attgctgctg ccagacagtc
     4081 agcaggcatt gccactgaaa ggatgactga agcgcataaa cagcttctgg ccctgcagca
     4141 gcgcatcagc gacctggacg ggaaaaagct gacggcagat gaaaagagtg tgctggcccg
     4201 taagaatgag ctgattcagg cgctgacgct gctggatgtg aaacagcagg agctgcagaa
     4261 acagacagcg cttaacgacc tgagaaaaaa aacggttcag ctgaccagcc agctggcaga
     4321 caaagaacgt gcactgcgtg agcagcacaa tctggatatt gccactgcag gtatggggga
     4381 taagcagcgg cagcgctacc aggcacagtt gcgcatccgg caggaatacc ggcaacagtt
     4441 gcaacagctt gagaatgaca gtcgccagaa aggcacttac gggacggagg actaccggag
     4501 ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg aatgaaaaca aacgctactg
     4561 gcaggaactg gaagtggcgc agggcgactg gaaaaacggt gccatgcggg cgtttcagaa
     4621 ttttacggcg gatgcggata atgcggcggg aacggcagaa cagatgttta cagtggcatt
     4681 cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc ggtaagctga attttaaatc
     4741 cttcacctct tccctgttgt cagatatggc cagaattatg gcacagatgg ccatgatgca
     4801 ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact aatgctgatg gcggtgttta
     4861 ccagtctgct gatttgagtc gctacagtgg cacggtggtt aaccgtccga cgttttttgc
     4921 ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct gaagccattc tgccactgcg
     4981 tcgcggtgct gatggtaagc tgggggttgt ggcggatact ggtggttcag gcatggtgat
     5041 gtttgccccg cagtacaaca ttgagatcaa taacgacggc acgaacgggc agataggtcc
     5101 ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg gccgcggact ttatgcaaca
     5161 gcaggcccgt gatggtggcc ggttaagtgg agcataccgg taatggagac gtttcactgg
     5221 aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag tggtgacagt gaagctgggc
     5281 gatggttatg aacagcgtcg tgcggcggga ctgaataacc agttgtcgac ttacagcgtg
     5341 acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag cctttctgga acggcacggt
     5401 ggcgtccgcg catttcagtg gacgccacct tatgactgga aactgatcag ggtggtttgt
     5461 cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg taacggccga ttttgaacag
     5521 gtggtgaact gatgcaggat atccggcagg aaacactgaa tgaatgcacc cgtgcggagc
     5581 agtcagcccg ggtggagctc tgggaaatcg atctgacaga ggtcggtggt gagcgttatt
     5641 ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac ctggcagggg cggcagtatc
     5701 aggcataccc cattcagggg tcggggtttg aactgaacgg caggggctgt gctgcccgtc
     5761 cgacactgac ggtctctaac ctgcacggca tggtcaccgg gatggcggaa gatctgcaga
     5821 gtctggtcgg cggaacggtg gtccggcgta aggtttacgc ccgttttctg gatgcggtga
     5881 acttcgtcaa cggaaacagc gacgccgatc cggaacagga ggtgatcagc cgctggcgca
     5941 tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt tgtgctggcc acaccaacgg
     6001 agacggatgg cgcggttttc ccggggcgta tcatgctggc gaatacctgt atgtggactt
     6061 accgttctga tgagtgtggt tacacgggca gggcagtggc tgacgagttc gacaaaccaa
     6121 caacggatat ccggaaggac aaatgcagca agtgtatgcg cgggtgtgag ttgcgcaaca
     6181 ataccggtaa tttcggcggt ttcctttcca tcaataaact ttctcagtaa atccatgaca
     6241 cagacagaat cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgtggc
     6301 ttcgtggtga gaacgccgga gggagacagg tatcttccca gcgagaatat ctccggtgag
     6361 ccggaggaac ggttccggat ggctccggag gactggctgc gggcacaaat gcagggtgag
     6421 attgtggcac tggtccacag tcatcccggt ggtctgccct ggctgagtga ggctgaccgg
     6481 cggctgcagg tgcagagtga tttgccgtgg tggctggtct gccggggggc gattcacaag
     6541 ttccgctgtg tgccgcatct caccgggcgg cgctttgagc acggggtgac ggactgttac
     6601 acgctgttcc gggatgctta tcatctggcg gggattgaga tgccggattt tcatcgcggg
     6661 gatgactggt ggcgtcacgg tcagaatctc tatctggata atctggaggc cacagggctg
     6721 tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca
     6781 tcggtgccga atcatgccgc catttactgc ggcgatggtg agctgctgca ccatattcct
     6841 gaacaactga gtaaacgaga gaggtatacc gacaaatggc agcgacgcac acactccctc
     6901 tggcgtcacc gggaatggca cgcatctgcc tttacgggga tttgcaacga tttggccgcc
     6961 gcatcgacct tcgtgtgaaa acgggggctg aagccatccg cgcactggcc acacagctcc
     7021 cggcgtttcg tcagaaactg agtgacggct ggtatcaggt gcgcattgcc gggcgtgatg
     7081 caggtgaaac cgaattgtct gcccgtctta atgagccgct ggcaaatggt gcagtgatcc
     7141 acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt gtttcaggcg gtgctggggg
     7201 cagctgttat ggcggttgct atatggatgc cgggggtagg aattatggcg agtaatctgc
     7261 tgttttctct cggtgccagt atgacgcttg gcggtgttgc acagatgctg gcaccgaaag
     7321 ccagaactcc ccgtacacag acaacggata acggcaaaca gaacacctgt ttctcctcac
     7381 tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa atgcgcgtgg
     7441 ggtcgcgggt ggtatctcag gagatcagca cggcagacga aggggatggt ggtcaggttg
     7501 tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc gcctgcgggc ggttttgtcg
     7561 tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga
     7621 aggacaacct gaaatccacg cagttgctga gtgtgattga tgccatcagc gaagggccgg
     7681 ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa cggtacgccg gtcctggata
     7741 gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt ccgcgccggc gagcaggagc
     7801 agacaccgcc ggaggggttt gaatcttccg gctcagagac tgtgctgggt acggaagtga
     7861 aatacgacac gccgatcacc cggaccatca cgtcggcaaa cattgatcgt ctgcgcctga
     7921 ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg ggaccggaat ccgtcggaag
     7981 tccgcctgct ggttcagata cagcgtaacg gtggctgggt gacggaaaaa gacatcacca
     8041 ttaagggcaa aaccacttcg cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc
     8101 gcccgtttaa tatccggatg cgcaggatga caccggacag caccacagac cagctgcaga
     8161 acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca
     8221 cggcgctggt cggcgtgcag gtggattcag agcagttcgg taaccagcag gtgagtcgca
     8281 attatcatct tcgcgggcgc attctgcagg tgccgtcgaa ctataacccg cagacgcggc
     8341 aatacagcgg tatctgggac ggaacgctta agccagcata cagcaacaac atggcctggt
     8401 gtctgtggga catgctcact catccgcgct acggcatggg gaaacgtctt ggtgcggcag
     8461 atgtggacaa atgggcgctg tatgtcatcg gccagtactg cgaccagtca gtgccggacg
     8521 gatttggcgg cacggagccg cgcatcacct gtaacgctta cctgaccaca cagcgtaagg
     8581 cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacgggc
     8641 agacgctgac gttcgtgcag gaccgaccgt cggataaagt gtggacctat aaccgcagta
     8701 atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc
     8761 gccataatgt cgttgaggtg aactggattg atccggacaa cggtcatgag acggcgacag
     8821 agcttgtgga ggacacgcag gccattgtcc gttacggtcg taacgtcacg aagatggatg
     8881 cctttggctg taccagccgg ggacaggcgc accgcgccgg gctgtggctg attaaaacgg
     8941 aactgctgga aacgcagacc gtggacttca gcgtcggcgc agaagggctt cgccatgtac
     9001 cgggtgatgt cattgaaatc tgcgatgatg attatgccgg tatcagcacc ggtggtcgcg
     9061 tactggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc acgctgccgt
     9121 cctccggtac cacgctgata agcctggttg acggaaatgg caatccggtc agcgtggagg
     9181 ttcagtccgt caccgacggc gtgaaggtga aagtgagccg ggttcctgac ggcgttgcag
     9241 aatacagcgt gtgggggctg aagctgccga cgctgcgcca gcgcctgttc cgctgcgtga
     9301 gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc cgtgcagcat gtgccggaaa
     9361 aagaggccat cgtagataac ggggcacact ttgacggtga ccggcgcggc acggtgaatg
     9421 gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt caccgcagac agcggggaat
     9481 atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa gggtgtgagc ttcctgcttc
     9541 gcctgaccgt ggcagcggac gacggcagtg agcggctggt cagcacagcc aggacgacgg
     9601 aaaccacata ccgcttcagg cagctggcgc tggggaatta cagtctgaca gtccgggcgg
     9661 taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc gttccggatt gcggcaccgg
     9721 cagcgcctgt cactattgaa ctgataccgg ggtattttca gataacggtg gtcccgaaac
     9781 tggctgtata tgacccgacg gtgcagtttg agttctggtt ttcggaaaag cggattgcgg
     9841 atatcaggca ggttgaaacc agcgcccgct atcttggcac ggcgctgtac tggatagccg
     9901 ccagtatcaa tatcaggccg ggccatgatt attattttta cgttcgcagt gtgaacaccg
     9961 ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag cgatgatgcg gaaggttatc
    10021 tgagttttta taaagggttg atcaataaaa cgcatctcgg caaggagttg tggacgcaga
    10081 ttgataacgg tcagcttgcg ccggacctga ctgaaatcag gacgtccatt acgaatgtca
    10141 gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa tcagagtgct gcaatccagc
    10201 agatacagaa agttcaggtt gatacaaata ataacctgaa cagcatgtgg gctgtgaagc
    10261 tgcaacagat gaaggacgga cgcctttata ttgcgtggta tcggtgccgg tattgagaat
    10321 acgccagcag gaatgcagag tcaggtgctg ctggcggcag acaggattgc gatgattaat
    10381 cctgcgaatg gcaacacaaa gccgatgttt gttggtcagg gcgatcagat attcatgaac
    10441 gacgtgttcc tgaaacgcct
//
LOCUS       DBKELA010000122        10439 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.122, whole genome shotgun sequence.
ACCESSION   DBKELA010000122 DBKELA010000000
VERSION     DBKELA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.122"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            159..506
                     /locus_tag="ACUQR0_003437"
                     /pseudo
     CDS             159..506
                     /locus_tag="ACUQR0_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            559..954
                     /locus_tag="ACUQR0_003438"
     CDS             559..954
                     /locus_tag="ACUQR0_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HHV9110328.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            995..1738
                     /gene="cmoA"
                     /locus_tag="ACUQR0_003439"
     CDS             995..1738
                     /gene="cmoA"
                     /locus_tag="ACUQR0_003439"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HHV9110329.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1735..2706
                     /gene="cmoB"
                     /locus_tag="ACUQR0_003440"
     CDS             1735..2706
                     /gene="cmoB"
                     /locus_tag="ACUQR0_003440"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HHV9110330.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="ACUQR0_003441"
     CDS             complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="ACUQR0_003441"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HHV9110331.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5325..6425)
                     /locus_tag="ACUQR0_003442"
     CDS             complement(5325..6425)
                     /locus_tag="ACUQR0_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HHV9110332.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="ACUQR0_003443"
     CDS             complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="ACUQR0_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HHV9110333.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7573..8139)
                     /locus_tag="ACUQR0_003444"
     CDS             complement(7573..8139)
                     /locus_tag="ACUQR0_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHV9110334.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8355..10088
                     /gene="argS"
                     /locus_tag="ACUQR0_003445"
     CDS             8355..10088
                     /gene="argS"
                     /locus_tag="ACUQR0_003445"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HHV9110335.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(10130..10174)
                     /locus_tag="ACUQR0_003446"
     CDS             complement(10130..10174)
                     /locus_tag="ACUQR0_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110336.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            10265..>10439
                     /locus_tag="ACUQR0_003447"
     CDS             10265..>10439
                     /locus_tag="ACUQR0_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HHV9110337.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYN"
BASE COUNT         2520 a         2689 c         2513 g         2717 t
ORIGIN      
        1 cataatgggc ttttaatcct atcgtttatt gcttgcatag tagaatctaa ccatccatca
       61 ctcatagcta accctcggaa atcttacatt tctgtctatc ataaccacta gcatcacctt
      121 taggaaccat ctgaaacggg aggtgtattt agaccttgct aatcttagac agccgcccgg
      181 ttcatgcatc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag
      241 ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc ggtagtgatg
      301 atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagcg cagtggcatc
      361 agaccactac gccttatctt tttttacata cgccagatat tgcccaggcc ccggaactgg
      421 tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta
      481 ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc
      541 attttaaagg gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta
      601 atgaagttct cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac
      661 ggtggtttta gcgaactgca aagcgctatt cgcattcatg gtaacgcggt ggaatatatt
      721 cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg
      781 catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac
      841 cgtctgtttc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg
      901 gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca
      961 atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc
     1021 tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtcgctg aagtcttccc
     1081 ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt
     1141 agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc
     1201 ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga
     1261 caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata aagcccctac
     1321 gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt
     1381 ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa
     1441 aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga
     1501 agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg
     1561 ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga
     1621 ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg
     1681 gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc
     1741 gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg
     1801 ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg
     1861 tccaacacgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc
     1921 gtaaccgccg aaagcgaaga accactgagc accgggcaaa ttaaacgcat tgaaacgctt
     1981 atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat
     2041 accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc
     2101 ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc
     2161 gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa
     2221 gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa
     2281 cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc
     2341 cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg
     2401 gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt
     2461 tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg
     2521 aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag
     2581 cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat
     2641 ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag
     2701 ccgtaaaggt ctggtattac tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
     2761 cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca
     2821 gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga
     2881 acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac
     2941 accagcgcag agttaccggc acaggcattt gcccgctgcg agctggggat atccgccgtt
     3001 aacacgttcg cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca
     3061 tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga
     3121 ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg agcagcatct
     3181 tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca
     3241 tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc
     3301 tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc
     3361 ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct tggcgtaccc
     3421 agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc
     3481 tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata
     3541 gtgacgcgtt gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc
     3601 atttcatctt taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga
     3661 tcggcaaaca cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc ctgcttcatc
     3721 ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat
     3781 gtggtgatcg gtaatacgat atctgcgtgt ttggctgctg cggtccagta gcattcagaa
     3841 acgacgatca tctccggttt ctgccaggct ttgatcagac gattagtatc ctggtgatgg
     3901 gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta
     3961 ccgttatgct gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga
     4021 atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac
     4081 atttccggca gcacgccgcc aacacgcgtc gggttgccac cattggagta atgataagag
     4141 aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc
     4201 atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt
     4261 gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta
     4321 atttccgccg tccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct
     4381 tcaaactgcg gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt
     4441 gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg
     4501 atccaggtgg cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca
     4561 atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta
     4621 ctgctccagg caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg
     4681 ctgttttcca gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc
     4741 acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa
     4801 ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa
     4861 gagcgccagc cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc
     4921 tcatgttgct catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct
     4981 tcgccacgac ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga
     5041 tgctgaatac gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt
     5101 atggttttcg ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc
     5161 atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg
     5221 agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat
     5281 tcacgtcttg ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta
     5341 atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccggttcgtt ttcgctcatg
     5401 ctggttcgtg cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg
     5461 gcggcaatag aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc
     5521 catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga
     5581 cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata
     5641 ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt
     5701 tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca
     5761 acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg
     5821 aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc
     5881 gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg
     5941 atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc
     6001 gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct
     6061 tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca
     6121 ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca
     6181 aataaataat ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct
     6241 ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg
     6301 caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac
     6361 ccgccgccac caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct
     6421 cgtatagatt cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca
     6481 acgaattaac cgcttgcata actcggcaca acactgcctg aaataatcga taaagaatat
     6541 gatttattac aatgtaatca ttaattgcta aggaataacc cagttgctat ttagaatata
     6601 tatttatcag ttttagcaat ttaaatccca taatcaatgt gaatataaaa aatattttta
     6661 gcacaacgct atgcgcactg tcacccgctc aacagatcac ggttatcgtt cgttttttat
     6721 actgctcagg gataaaaaaa ggcccctgtt gaaattgcag gggcctggta cgagcaagca
     6781 tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg ttcaatgatt
     6841 cctttcattt cagcaaccgc cgccccgtct acgatataac gcgaatactc gtccgcgtgt
     6901 tcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc ttgccacgct
     6961 cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag gttttctgca
     7021 cggactcctg ctccagccat aatgattgga gcatcacgat gggcaataag ttccataatt
     7081 tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg ggcaatgccc
     7141 aattccgcaa gattattgag tgtatataaa gggttagcgc acatatcgaa ggcgcgatga
     7201 aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg catatcgaca
     7261 ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc gcggaccgtg
     7321 cgcacatcct caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt
     7381 gggcgaatta tcggatgcac agggatcgtc acccgctggc gcacggattt cagtaccccc
     7441 aacgacggcg ttaagccccc ctcttttggg gctgcgcata attcaactct gtctgcgccg
     7501 ttttgctgcg ccgttagtgc acattccatg ctgtaacagc aaatttccag taatgccatt
     7561 tttgctcctt aattacgctg actgctcgct ggcaacgatc tcttcaatgg accacggatg
     7621 aaacttaata gttgttttgc catcggtgac cgccagcgtc ggattcggta atcgctcatg
     7681 ctcacctttt ggtgaactgg ttttaactga gatccccggt aggctgagtc cttcatcaga
     7741 aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggcagaa cgatttcaat
     7801 atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct ccacattgga
     7861 aaactgccag tgcgcaacct gtaccggttc atgcaattta aacagacaaa tcggtctgcc
     7921 attgatcatt ttttctgaca aaagctcgcc acactgttca aaaccgcgac gccagcgctc
     7981 agcggtgacg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc
     8041 caaccccaga cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta aatccgatgc
     8101 aatatcctgc agctcgtcga tagattgcca gttcgccata atcactcttc gtctttcagt
     8161 aaaagcgtta atttaccctg ttgccctgtg ccaaccaacc gctgatttca cgccgcttct
     8221 gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa cagccatttg
     8281 cagtatactc ccgccctaat ttctttaact ggtgcgggca atttttgctc gcttcatcaa
     8341 tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt caggccatga
     8401 ttgcggcagg cgcgcctgcg gattgcgaac cgcagattcg tcagtcagca aaagttcagt
     8461 tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt atggcaccgc
     8521 gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc agcaaagttg
     8581 agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg gctgaacatg
     8641 ttcagcaggc gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa cagaccattg
     8701 tggttgacta ctctgcgcca aacgtggcga aagagatgca cgtcggtcac ctgcgctcta
     8761 ccattattgg tgacacagca gtgcgtactc tggagttcct cggtcacaaa gtgattcgcg
     8821 caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg ctggaaaagc
     8881 agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc taccgcgatg
     8941 cgaaaaagca ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa
     9001 aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc gacatcacca
     9061 tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga
     9121 tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc aaagccaaag
     9181 gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt aaaaacaagg
     9241 aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc tacaccacca
     9301 ctgatatcgc ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc gtgctgtatt
     9361 acatcgactc ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc cgtaaagcag
     9421 gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg ctgggtaaag
     9481 acggcaaacc gttcaaaacc cgcgcgggtg gtaccgtgaa actggcagac ctgctggatg
     9541 aagccctgga gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg ccagctgacg
     9601 agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg gatctctcca
     9661 aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt gagggtaata
     9721 ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt aaagcagaaa
     9781 ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt gaagcgcaac
     9841 tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc
     9901 cgcatgtaat gtgtgcttac ctgtacgatc tggccggtct gttctctggc ttctacgagc
     9961 actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac
    10021 aactgacagc gaagacgctg aagctgggtc tggatacgct gggtattgag actgtagagc
    10081 gtatgtaatc gatttttcgt gagagtgaag cctgatcagg gttagccgat caggcttttt
    10141 tattgccatc taaatgtatt ctgaaaacgg acatgccatt gttttctcac tgttgagtaa
    10201 gaggccagaa gcgtaatatc caggcccagg aaaacgataa cggttcaatt taaggaatac
    10261 cgaagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg cttacgcaga
    10321 agatacggtt atagtaaacg accatgacac ttcagccatc aaagattgtt ggcaaaaaaa
    10381 ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag aatacaatc
//
LOCUS       DBKELA010000123        10306 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.123, whole genome shotgun sequence.
ACCESSION   DBKELA010000123 DBKELA010000000
VERSION     DBKELA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.123"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(337..582)
                     /locus_tag="ACUQR0_003448"
     CDS             complement(337..582)
                     /locus_tag="ACUQR0_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110338.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="ACUQR0_003449"
     tRNA            complement(1227..1302)
                     /locus_tag="ACUQR0_003449"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="ACUQR0_003450"
     CDS             complement(1408..2115)
                     /locus_tag="ACUQR0_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HHV9110339.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2268..2351
                     /gene="yqgH"
                     /locus_tag="ACUQR0_003451"
     CDS             2268..2351
                     /gene="yqgH"
                     /locus_tag="ACUQR0_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HHV9110340.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="ACUQR0_003452"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="ACUQR0_003452"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HHV9110341.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="ACUQR0_003453"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="ACUQR0_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HHV9110342.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="ACUQR0_003454"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="ACUQR0_003454"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HHV9110343.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="ACUQR0_003455"
     CDS             complement(7299..7574)
                     /locus_tag="ACUQR0_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HHV9110344.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="ACUQR0_003456"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="ACUQR0_003456"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HHV9110345.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="ACUQR0_003457"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="ACUQR0_003457"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HHV9110346.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="ACUQR0_003458"
     CDS             9534..9860
                     /locus_tag="ACUQR0_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HHV9110347.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..>10306
                     /locus_tag="ACUQR0_003459"
     CDS             10044..>10306
                     /locus_tag="ACUQR0_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 family protein"
                     /protein_id="HHV9110348.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWI"
BASE COUNT         2515 a         2509 c         2712 g         2570 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattga
//
LOCUS       DBKELA010000124        10206 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.124, whole genome shotgun sequence.
ACCESSION   DBKELA010000124 DBKELA010000000
VERSION     DBKELA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.124"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(123..1472)
                     /gene="lysC"
                     /locus_tag="ACUQR0_003460"
     CDS             complement(123..1472)
                     /gene="lysC"
                     /locus_tag="ACUQR0_003460"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HHV9110349.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1564..1760)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1997..3646
                     /gene="pgi"
                     /locus_tag="ACUQR0_003461"
     CDS             1997..3646
                     /gene="pgi"
                     /locus_tag="ACUQR0_003461"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HHV9110350.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4108..4350
                     /gene="yjbE"
                     /locus_tag="ACUQR0_003462"
     CDS             4108..4350
                     /gene="yjbE"
                     /locus_tag="ACUQR0_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HHV9110351.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4464..5102
                     /locus_tag="ACUQR0_003463"
     CDS             4464..5102
                     /locus_tag="ACUQR0_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HHV9110352.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5099..5836
                     /locus_tag="ACUQR0_003464"
     CDS             5099..5836
                     /locus_tag="ACUQR0_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HHV9110353.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5836..7932
                     /locus_tag="ACUQR0_003465"
     CDS             5836..7932
                     /locus_tag="ACUQR0_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HHV9110354.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7979..8257)
                     /locus_tag="ACUQR0_003466"
     CDS             complement(7979..8257)
                     /locus_tag="ACUQR0_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110355.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8258..8401
                     /locus_tag="ACUQR0_003467"
     CDS             8258..8401
                     /locus_tag="ACUQR0_003467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110356.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8527..8937
                     /gene="psiE"
                     /locus_tag="ACUQR0_003468"
     CDS             8527..8937
                     /gene="psiE"
                     /locus_tag="ACUQR0_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HHV9110357.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8981..>10206)
                     /gene="xylE"
                     /locus_tag="ACUQR0_003469"
     CDS             complement(8981..>10206)
                     /gene="xylE"
                     /locus_tag="ACUQR0_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HHV9110358.1"
                     /translation="RDSLKISAVLFFISGVGSAWPELGFTSINPDNTVPIYLAGYVPE
                     FVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIA
                     RSGDASWLNTDGRRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQTESILRKIM
                     GNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPE
                     VFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLG
                     TAFYTQASGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLA
                     NYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFVPETKGKTLEELEA
                     LWEPETKKTQQTATL"
BASE COUNT         2544 a         2646 c         2660 g         2356 t
ORIGIN      
        1 tcattattag ctccttctga tgccattcta tttcaggaag gagtgtccgt taaactcagg
       61 ctacctcata gtgacaagaa aatcaatacg gcccgaaata tagcttccag gccatacagt
      121 atttactcaa acaaattact atgcagtttt tgcaccacct gctcggcatc ttcgccgggc
      181 accaggaagc acaggttatg gctggatgcg ccgtaacaaa tcatgcgaat gttgaacggt
      241 tccagtacgc cgaatacctc tttgccaacg ccgcaggctt ttgacaggtc attgccaatc
      301 aacgcgacca gcgccagacc ttcttccacc tccacccggc acagtgcgga aagctccatc
      361 agcagagatt gcgtcagcaa cgtatcgcca gtggaggttg aaccggtggt atcaagggtt
      421 aatgccacgc tcacttctga cgtggtgatt aagtctaccg aaatattatg ccgcgcgagg
      481 atgccgaaaa cttccgcgag gaagccgcga gaatgcagca tattcaggct gtgcaaagtg
      541 agcagagtct gattgcgacg aagcgccagc gcgcggaaca gcggcggatt ttcagtttta
      601 ttgcacacca gcgtaccacc tgcgcgtggg tctttgctgg agccgacaaa gaccgggata
      661 tcgctgcgta ctgcgggtag caacgttgcc ggatgcagta cttttgcacc aaaagttgcc
      721 atctctgccg cttcggcaaa cgcgatttca tcaatgcgtt ttgctgcgga aactacgcgt
      781 ggatcggtgg tgtagatgcc cgggacgtcg gtccagatat caacacgaga tgcgtgtaaa
      841 gcctccgcca gcaaggctgc cgtataatcg ctgcctccac ggccaagcgt cgttgtacga
      901 cctttatttt cgctaccgat aaatccctgg gtgatcacta agccttcatt gaggcgtggg
      961 agcagctgca gcgcggccag ttccgccagc gcggctacat ctggctctgc acgaccaaat
     1021 cggtcgttgg tacgcatcac tttacgtaca tcaaaccact gtgcctgaac atcgcgttcg
     1081 cgcaggattt cgacaaacag cagggtcgac atcagctcgc cgtggctgac cagctcatct
     1141 gtcagcgccg gagacgttgc cagcgccgcc gcttctgcca gaacagtaat gttctccagc
     1201 agacgttcaa tctcttcacg gataacgttc gggtaacgca gacgttccag aatggcaaac
     1261 tggatgttgc gaatagcgtc gagtttttcg aatcgctcgc caagttccag cccttccgct
     1321 aaagcgacca gcagattagt gataccagca gaagccgaga ggactactaa acgcacgttg
     1381 gcatcagaaa gcacaatatc agcgctgcgg ttcatggcgt caaaatcagc tacgctggta
     1441 ccgccaaatt tggagacaac aatttcagac ataactacct cgtgtcaggg gatccatttt
     1501 cagccttggc acaagggaag agcggaagac gggtgggcgc agagcgatac ttcgctacta
     1561 ttttcaccca gaagtgctcc accacttgcg aaacgcccga ctgcgaacgc ttctggtgac
     1621 aacccagggg attcagcccc tgtagccgat gatgaacgtg gccagccgtt caatcacctc
     1681 ggcgatgcac cccctcaggt gttatcacag gactggctcc tccaacaccg ttacttgggc
     1741 aacgcgcctc ttctggcctg cgctagcgca ggtagtacat ttataaataa agggtgagcg
     1801 gggcggttgt caacgacggg gtcatgcgga tttttcatcc actcctggcg gtcagtagtt
     1861 cagctaataa atgcttcact gcgctaaggg tttacactca acattacact aacggcacta
     1921 aaaccatcac atttttctgt gactggcgct acaatcttcc aaagtcacaa ttctcaaaat
     1981 cagaagagta ttgctaatga aaaacatcaa tccaacgcag accgctgcct ggcaggcact
     2041 acagaaacac ttcgatgaaa tgaaagacgt tacgatcgcc gatctttttg ctaaagacgg
     2101 cgatcgtttt tctaagttct ccgcaacctt cgacgatcag atgctggtgg attactccaa
     2161 aaaccgcatc actgaagaga cgctggcgaa attacaggat ctggcgaaag agtgcgatct
     2221 ggcgggcgcg attaagtcga tgttctctgg cgagaagatc aaccgcactg aaaaccgcgc
     2281 cgtgctgcac gtagcgctgc gtaatcgtag caataccccg attctggttg atggcaaaga
     2341 tgttatgcca gaagtcaacg cggtgctgga gaagatgaaa accttctcgg aagcgattat
     2401 ctccggtgag tggaagggtt ataccggcaa agcaatcact gacgtagtga acatcgggat
     2461 cggcggttct gacctcggcc cgtacatggt gaccgaagca ctgcgtccgt acaaaaacca
     2521 cctgaacatg cactttgttt ctaacgtcga tgggactcac atctcggaag tgctgaaaaa
     2581 agtaaacccg gaaaccacgc tgttcctggt agcatctaaa accttcacca ctcaggaaac
     2641 catgaccaac gcccatagcg cgcgtgactg gttcctgaaa gcggcaggtg atgaaaaaca
     2701 cgttgcgaaa cattttgcgg cgctctcaac caatgccaaa gccgttggcg agtttggtat
     2761 tgatactgcc aacatgttcg agttctggga ctgggtcggc ggccgttact ctttgtggtc
     2821 agcgattggc ctgtcgattg ttctctccat cggctttgat aacttcgttg aactgctttc
     2881 tggcgcacac gcaatggaca agcatttctc caccacgcct gccgagaaaa acctgcctgt
     2941 cctgctggcg ctgattggca tctggtacaa caatttcttt ggtgcggaaa ctgaagcgat
     3001 tctgccgtat gaccagtata tgcaccgttt cgcggcgtac ttccagcagg gcaatatgga
     3061 gtccaacggt aagtatgttg accgtaacgg taacgttgtg gattaccaga ctggcccgat
     3121 tatctggggt gaaccaggca ctaacggtca gcacgcgttc taccagctga tccaccaggg
     3181 aaccaaaatg gtaccgtgcg atttcatcgc cccggctatc acccataacc cgctctctga
     3241 tcaccaccag aaactgctgt ctaacttctt cgcccagacc gaagcgctgg cgtttggtaa
     3301 atcccgcgaa gtggttgagc aggaatatcg cgatcagggt aaagatccgg caacgcttga
     3361 ctacgtggtg ccgttcaaag tgttcgaagg taaccgcccg accaactcca tcctgctgcg
     3421 cgaaatcact ccgttcagcc tgggtgcgtt gattgcgctg tatgagcaca aaatctttac
     3481 tcagggcgtg atcctgaaca tcttcacctt cgaccagtgg ggcgtggagc tgggtaaaca
     3541 actggcaaac cgtattctgc cagagctgaa agatgataaa gaaatcagca gccacgatag
     3601 ctcgaccaat ggtctgatta accgctataa agcgtggcgc ggttaatcat catgcgtgcc
     3661 tgatgccgat atgtaggccg gataaggcgt ttacgccgca tccggcaacc ggagcctgat
     3721 gccaatatcg acattaatgc atagtatgag gagttaaata accttaaaaa caatgccgac
     3781 aataagtcgg cattgtttta tcagataaat ccccttgtct gtgatttaac ggaaatcata
     3841 ccgtgaggtt aatcctaaaa tagattttta atcgttgttt atttcggaga atacgcagat
     3901 taattacttt tgtttttatt ttaagcttat gatttttatt gttatttaaa tataagttga
     3961 aacttatatt tgatattcat tccaattatc ctaaaactcc atcgctaatt ccccgcgccg
     4021 taattcgcat gctttagttg tgtatactcg atcccgcccg aaatgttttt gggtaaatct
     4081 ccattcattc aatgaaggga aattgttatg aaaaaagttc tgtatggcat ttttgccata
     4141 tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac aggtgggcga agcggcagag
     4201 tcggcagcaa cgtcggtttc ggcggggagt tcctccgcga ccagcgtcag caccgtaagc
     4261 tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg atggttctaa taccgggacc
     4321 atcacaacca cgaccaccag tacccagtaa taaagtatgt atccccaaaa taatttgagt
     4381 cattgcatct gtggcttgaa gtatgaaggg atttaccata accacactcc ggtgtggtta
     4441 ttctgcccct ctggagaaga gtcgtgaagc gacctgcact cattcttatc tgcctgctat
     4501 tacaggcctg ttcagccacg actaaagagc tgggcaattc actgtgggac agtctgttcg
     4561 gcacgccagg cgtacagctg acggacgatg atattcaaaa tatgccctac gccagccagt
     4621 acatgcagct taatggcggg ccgcaattgt ttgtggtgct ggccttcgct gaagacggac
     4681 aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac acaacatggt cgtctggtga
     4741 agactttgct tggcggcgac aacctgattg aagtgaataa ccttgccgcc gacccgctga
     4801 ttaaacccgc acaaattgtt gatggtgcaa catggacgcg cacgatgggc tggaccgagt
     4861 accagcaggt acgctacgcc accgcacgct cagtcttcaa atgggatggc accgataccg
     4921 tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga cgaagaagtc tccaccgacc
     4981 aggcgcgctg gcataaccgc tactggatcg acagcgaagg gcaaattcgc cagtcggaac
     5041 agtatctcgg cgcggattat ttcccggtga aaaccactct catcaaggcg gcaaaacaat
     5101 gattaaacaa actattgtcg cgttgctttt gagtgtggga gcgtcatcgg tcttcgcggc
     5161 aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc aaaacgctaa cgggcgcaga
     5221 gcatttaatc gatctggtag ggcaacctcg actggcaaac agctggtggc ctggtgcggt
     5281 gattagcgaa gagctggcaa cggcggcagc attgcgtcag cagcaggcgt tgctgacacg
     5341 gctggcagaa cagggcgcag attccagcac cgacgatgcc gccgccatta acgccttacg
     5401 ccagcaaatc caggcattga aagtgacagg caggcaaaaa atcaatcttg atcccgatat
     5461 cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc aactacacgc tgtgggtcgg
     5521 accaccgccg tccacggtca cgttgttcgg gcttatcagc catcctggca atcagccatt
     5581 cactccaggt cgcgacgtgg cgagctatct ctctgaccag agcctgctca gcggtgcgga
     5641 tcgcagctac gcgtgggtcg tttacccgga cggacgcacg caaaaagcgc cggtggctta
     5701 ctggaacaag cgtcacgtag agccgatgcc cggcagcatt atttatgttg gcctcgcaga
     5761 ttccgtctgg agtgagacgc ctgatgccct taacgccgac attcttcaga ctctgacgca
     5821 gcggataccc caataatgaa aaaaagacat ctgcttagct tactggcgct gggcattagc
     5881 acagcttgct acggcgaaac atatcctgcg cccattggtc cgtcgcagtc ggatttcggt
     5941 ggcgtaggat tattacaaac tcccaccgcg cgtatggcac gggaagggga gttgagtctg
     6001 aactatcgcg ataacgatca gtaccgttat tactcagctt cagtgcaact cttcccgtgg
     6061 ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc agtacagcag cgtcgaagcg
     6121 ttctctggcg atcaaacgta taaagataaa gccttcgatc tcaaactgcg tttgtgggaa
     6181 gagagttact ggctgccgca agtggcggtt ggtgcgcggg atatcggcgg tacggggctg
     6241 tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc cgttcgattt tacgctcggc
     6301 ctgggctggg gatatctggg caccagcggt aatgtaaaaa atccgctctg ttcagccagt
     6361 gataaatatt gctatcgcga taacagctac aaacaggcgg gatctatcga cggtagccag
     6421 atgttccacg gtcctgcctc actgtttggc ggcgtggaat accagacgcc ctggcaaccg
     6481 ctgcgtctga aactggagta tgaaggtaat aattatcagc aggattttgc cgggaagctg
     6541 gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg ttaccgattg ggccgacgtt
     6601 aaccttagct atgaacgtgg caacaccttt atgtttggcg tcacgctgcg caccaacttt
     6661 aacgatctgc gcccgtccta catcgataac gcccgcccgc aatatcaacc gcagccgcag
     6721 gatgccattt tgcagcattc ggtagtggcg aatcagttaa cgttgctgaa atacaacgcc
     6781 ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc tgtatgttac cggcgagcag
     6841 gtgaaatatc gtgattcgcg cgaagggatc atccgtgcta atcggatcgt gatgaacgat
     6901 ctgccggatg ggatcaaaac gatccgcatt acggaaaatc gccttaacat gccgcaagtg
     6961 acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg ccggagagcc gttgggccac
     7021 gaaacgacgc tggcgcacaa acgcgtcgag ccagtggttc cgaagtccac cgagcagggc
     7081 tggtatatcg acaaatcacg ctttgatttc catatcgatc cggtgctgaa ccagtcggtc
     7141 ggtggcccgg aaaactttta catgtatcag ctgggcgtga tgggaacggc agatttgtgg
     7201 ctgacggacc atctactgac caccggcagc ctgtttgcaa atcttgccaa caactacgac
     7261 aagtttaact acaccaatcc accgcaggac tcgcacttac cgcgcgttcg tacccatgtg
     7321 cgcgagtatg tgcagaacga tgtctatgtg aataacctgc aagccaacta cttccagcat
     7381 ctgggcaacg gcttctacgg tcaggtctat ggaggttatc tcgaaaccat gtttggcggt
     7441 gcgggggccg aagtgttgta tcgtccgctg gacagtaact gggcgtttgg tctggatgcc
     7501 aactacgtta aacagcgcga ctggcgtagc gcaaaagata tgatgaaatt caccgactac
     7561 agcgtgaaaa ccggacatct gaccgcctac tggacgccat ctttcgccca ggacgtgttg
     7621 gtgaaagcca gcgtcgggca gtatctggca ggggataaag gcggcacgct ggagatcgct
     7681 aaacgctttg acagcggcgt ggtggtgggt ggctatgcca cgatcaccaa cgtttcgaaa
     7741 gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg tctcggtacc gttggatctc
     7801 ttctcgtctg gcccgacacg cagccgtgcg gcgattggct ggacgccgct gacgcgtgac
     7861 ggtggtcagc aacttgggcg taagttccag ttgtatgaca tgaccagcga ccgtagcgta
     7921 aatttccgct aagtcatggg aaaggtgcca gttttcgcac tcactggcac cctcattctt
     7981 aatgacctgt tgagccatca ggaacgcagt tcacccccgg tttacgatct ggtgtgtaaa
     8041 ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg caggtctggt tgcctgagcg
     8101 actgggccat cattgggctg gagacattgc tttgcggtag gtcggcttta tcagcagtac
     8161 cgagcggacc agcataagca ggaagaacag agactgataa catcaaagca gcaaaataag
     8221 gcttcatttt taccaccttt atcaggttac gtttcatttg ttccagagga acattgtcga
     8281 tttttcgcgc attgctggtg gctgggaatc acctgaatgg gtgatttttg aattaccggc
     8341 tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg gcttatccgg cctacgggta
     8401 ggtatatccg gctttggtgg aggcgcgctc caaatccagg ttgaacaaaa catacacaaa
     8461 aaatatagat ctccgtcaca tttttgcgtt atacaggaag ctcgccactg tgaaggaggt
     8521 actgctatga cgtcactctc tcgtccgcgc gtggagttta tctccaccat tttgcagacc
     8581 gtactcaatc ttggcctgct gtgcctcggc ctgattttgg ttgtcttcct cggcaaagaa
     8641 acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa ccagcaaata tgagctggta
     8701 gaaggactgg tggtttactt tctctatttc gaatttatcg cgctgattgt gaagtacttt
     8761 cagtccggtt ttcacttccc gctacgttac tttgtctata ttgggatcac cgccattgtg
     8821 cgcttgatca tcgtcgatca taaatcgcca ctggatgtgt tgatctactc ggctgcgatc
     8881 ctgttactgg tgatcaccct gtggctgtgt aattcgaaac ggctgaagcg ggagtaaaaa
     8941 gacagcacgc cgaaatggcg cggcgtgctg gacaggaaga ttacagcgta gcagtttgtt
     9001 gtgttttctt cgtttccggt tcccagagcg cttccagctc ctcaagggtt ttacctttgg
     9061 tttccgggac aaatttccac ataaacagtg ctgccagaac gcccatacaa ccgtaaatcc
     9121 agtaggagaa accgttgtgg aaatgggcca ccagccagga gtttttgtcc atcatcggga
     9181 aggtccagga gacgaagtag ttcgccagcc actgggccgc caccgcgatt gccagcgctt
     9241 taccacgaat agcattcggg aagatttccg acagcagtac ccagcatacc ggaccccagg
     9301 acatggcaaa ggcggcaaca tagaacagca tcgacagtag cgccacaata cccgatgcct
     9361 gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc cattccgagt gcgccgataa
     9421 tttgcagtgg cttacgacca aatttatcca cagtcataat tgccagtacg gtaaaggtga
     9481 ggttgataac tccgacaata atggtctgca acagcgcgat atccgtgctg gcccccagcg
     9541 ttttgaacac ttccggcgcg tagtacagca ccacattgat gccgacaaat tgctggaaga
     9601 tggagagcat aacgccgatt acaatcacgc ccacgccaaa catcagcaga cgaccaccgg
     9661 ttttgcggcc atgatccagg gagtgtttaa tttcctgtac tgcctgagtt gcaagcgtgt
     9721 tgcccataat tttgcgcagg atactttccg tctgttcttg cttgccgcgt gacatcagcc
     9781 agcgaggact ttctggcacg gtatacagca gcattaagaa cagcagtgca gggatacatt
     9841 ccgaggcaaa catataacgc ctgccgtcag tattcagcca gctggcatca ccggaacggg
     9901 caataaaata gtttacgcag taaactaaaa gttgcccgaa aataatcgca aactggttaa
     9961 atgaaaccag ttttccgcga atatgagctg gagccagttc cgcaatatac attggtgaaa
    10021 gcattgaggc taaaccaacg ccaataccgc caataatacg ataaataaca aattccggga
    10081 cataacctgc cagataaata ggtactgtgt tgtccgggtt tatagaggta aaaccaagtt
    10141 ctggccaggc agaacctaca ccagaaataa aaaacaggac agcagaaatc ttaagtgaat
    10201 cacgac
//
LOCUS       DBKELA010000125        10158 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.125, whole genome shotgun sequence.
ACCESSION   DBKELA010000125 DBKELA010000000
VERSION     DBKELA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.125"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..677)
                     /locus_tag="ACUQR0_003470"
     CDS             complement(<1..677)
                     /locus_tag="ACUQR0_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHV9110359.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMAARIVIMKDGIVQQVGA
                     PKTVYNQP"
     gene            complement(691..1350)
                     /gene="pgmB"
                     /locus_tag="ACUQR0_003471"
     CDS             complement(691..1350)
                     /gene="pgmB"
                     /locus_tag="ACUQR0_003471"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HHV9110360.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1347..3614)
                     /gene="ycjT"
                     /locus_tag="ACUQR0_003472"
     CDS             complement(1347..3614)
                     /gene="ycjT"
                     /locus_tag="ACUQR0_003472"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HHV9110361.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3611..4665)
                     /locus_tag="ACUQR0_003473"
                     /pseudo
     CDS             complement(3611..4665)
                     /locus_tag="ACUQR0_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(4675..5450)
                     /locus_tag="ACUQR0_003474"
                     /pseudo
     CDS             complement(4675..5450)
                     /locus_tag="ACUQR0_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(5468..6520)
                     /locus_tag="ACUQR0_003475"
     CDS             complement(5468..6520)
                     /locus_tag="ACUQR0_003475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HHV9110362.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6551..7393)
                     /locus_tag="ACUQR0_003476"
     CDS             complement(6551..7393)
                     /locus_tag="ACUQR0_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHV9110363.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7380..8261)
                     /locus_tag="ACUQR0_003477"
     CDS             complement(7380..8261)
                     /locus_tag="ACUQR0_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHV9110364.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8282..9565)
                     /locus_tag="ACUQR0_003478"
                     /pseudo
     CDS             complement(8282..9565)
                     /locus_tag="ACUQR0_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(9579..>10158)
                     /gene="ycjM"
                     /locus_tag="ACUQR0_003479"
     CDS             complement(9579..>10158)
                     /gene="ycjM"
                     /locus_tag="ACUQR0_003479"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HHV9110365.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2389 a         2833 c         2546 g         2390 t
ORIGIN      
        1 ggttggttat aaacggtttt cggcgcacct acttgctgaa caatcccgtc tttcataatc
       61 acaatccgcg ccgccatggt catcgcttcg gtctgatcgt gggtcacgta gatcatggtg
      121 gtgttcagtt tctgatgcag cttgctgatc tctgcacgca tttgtacgcg cagcttggca
      181 tcaaggttag agagcggttc atccattaaa aacacgcccg cttcgcgcac aatcgcccgc
      241 ccgagcgcca cacgctggcg ttgcccgccg gaaagcgcgc ccggcttgcg tttcaggtac
      301 tcacgcaggc cgagaatttg cgccgcccag ttcacccgct catcaatcac ctctttggcg
      361 attttttgca tcttcaggcc aaacgccatg ttgtcgtaaa ccgtcatatg cggatacaac
      421 gcgtagttct ggaacaccat cgcgatattg cgtgctttgg ctggaacgtc attcattcgt
      481 ttgccgtcga tcaacagatc gccgccgctg atctcctcaa gcccggcaat catgcgcagg
      541 gtggtcgatt taccgcagcc cgacgggccg acaaatacga tgaactcttt atcggcaatt
      601 tccaggttga agtccttcac cacatgcacc tggttatcgt agattttttg aatatgttgt
      661 aacgaaagct gagccatgtt gattcctttg ctatacgttt tgccagaagg ccgataaccg
      721 cgaccaggtc agggagtccg ttgagggcaa cagtaattgc gccccggtta agcccgcacc
      781 gatccccacc gagcgcatac cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc
      841 aatgccgata catgcctgcg gcggcacgcc cagcccggca caggcagcga gaaagatttc
      901 cggatccggt ttcgagtttt taagttggga agcatccgcg cagaaggtga aaaactcgcg
      961 cagctccagc gccgctaaaa tcgtcggcgc attcagggag acggaagcca gccctaccgg
     1021 aatctgctgt gcacggagct ctgccagcaa attgcgaata ccgggcagca cagcattaac
     1081 cgtcagctcg cgcagtgagt ggacgtaaag cagatttttg cgatacgcca gttgcgcccg
     1141 ctcctgcggg ttaaagtcgc cctctttgcc cccgtgttgc agaatgcgcc gcagagactc
     1201 atcgcggctg atccctttta gagattcgtt aaactgcgca tcaatactga tgccaatttc
     1261 agcggcaatc tgctgccacg cctggaaatg cagatgcgcg gtatcggtga ttacaccatc
     1321 cagatcgaaa attacccctt gcagtttcat tcatcctcct gatgcgtggt agcggtccca
     1381 ttgaagggca aaataaaatc acccaaacag aaaacagatt cttcagccac ggatataagc
     1441 tgaccgttca aacgcagtga aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtcg
     1501 agagtgacct gtaattcgca gccctgccag aacagaggga aagacaactg ttgccactgc
     1561 tcaagtaacg ccggatttag atgcaattca ccgtcacgca cgctcacccc ggcaaaaccc
     1621 tgaatcgccc ccagccagat agcgccagtt gcggcagcat ggataccatc atcacaacta
     1681 tgcggatcag caccaagatc gatttcagtc ccctcgcgcc agaactgata actttgggtc
     1741 agcaggccac agcgtgcagc aacaatgccg tggattgctt tacttaatga cgagtcgtga
     1801 atagtgcgcg gttcataaaa ttgcagattg gcaagacacg atgccgatga gaactgctct
     1861 ggcagcatgt aattgagcat caccacatca gcttgtttga ggatctgcat ctcgttcact
     1921 tctgcgcgtg aataatccag cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc
     1981 agattaatcg ccggcttagt cataaacgaa tcatcctgcg gcaaaacacc gtcgggctga
     2041 atttctggca gccgcagttc tttaaggaac atttcggcac gatgaataaa cgcgtcgtca
     2101 ctacagccaa actggcgggc aatactcagc gcctgctgga cgttgtaata caccatatag
     2161 ctggtgaagg cgttattatt gacatgttcg gtatattcgt ctggcccaat aacatcatga
     2221 atttccagac ggtcgttaac cctcaccgcg cggctaatcc agaactttgc agtttccaga
     2281 agtagcgcca tgccttcatg cgcaatgaaa ctttcatccc ccgtggtctg ccagtattga
     2341 ataaccgccc aggcgatatc ggccaccaga tgatgttccg cctgcgccga ggccactttt
     2401 tgccgcagcc cggtgcgaat gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg
     2461 cgcgcgcttt cccacggaaa tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc
     2521 gcgcctggca agttgtgcca gcgataacgc agtaaacttc gggcaaccgt cggatcgcta
     2581 aacagatgga acggcagcaa aaatacttct gtatcccaga aaacgtggcc tttgtagcct
     2641 tcgccggtta agccttttgc cgcaatactg ctgcgctcgc cgtgagccgg cgtcatgatg
     2701 cgcagatgat aaagcgcata atctaacgct tgctgatcgt gcgcatcgcc gccatttacc
     2761 gtgatacgac gtttctgcca ccattgacgc cagttttctg ttgatgctgc aagatgttgg
     2821 tcgtaactct gttgcgcgca catttcaagc tggcgaagcg acacgctgcc ccactcgtct
     2881 aaagcagctt gcctgtcatc ccgccagtcg atccacacca gtttttgcaa cgtcactgtc
     2941 tcgcctgcat gaagctgcgt actggtatgt tgcagtaaac ggcgctcttt ggcggtatag
     3001 cattgctgca catcaccgct cacctggcag caacaactga tggccacatc actgcgccca
     3061 tcctgggtgg tgtagatccc ctgcatcaaa tgctgaccaa acacccgcac ctgggtttcg
     3121 tcgagatgct ggcgaccgtg gttggtttgc gtggcgtcaa tgcctgttga aatcagcact
     3181 gacgcgtcgg cgtccagtgg cataatagtg atttccagcg caatgagcgg cagttggtct
     3241 gccgaaacaa agcgacggct ggcgatggtg taacctgcgc cgttgctggt acgccagaca
     3301 acgctacggc gtaattctcc actggcaaag tcaagttcac gctgccaggc ttcgcgggat
     3361 aacgagaaaa cctcaccatt tatggcaatc tccatcccca agatatcagg cagattcacc
     3421 agttcgttga tttcaccttt tcccgcccga tgatacagcc ccgccagata catccctcgc
     3481 gtctggcgcg tgtaatcttc ttcatggctg gcgcgaagcc caagatagcc gttcccctgc
     3541 gccatcagcg atgcatactt gttcagggta tgctggctga aatggggttc tgataacgtt
     3601 actggcctgg tcataattcc acacgcgtcc ctgtttctgc ggattgatat aacgccgcca
     3661 ccagttgctg gatgatgtac ccctgctcgg cgtcggctat catcacgggc ttgccctgta
     3721 cgtgattgat aaaggcttcc atgctgcgca aatggcggtt gtcgtctgcc atttcccgtt
     3781 gcatcagcgt cattaattca ccgttgttat cggtgtagat atgtgctgga aacagcgtcg
     3841 caccggcttt atcaccacaa aagctgacgt tcataatcaa ctgttcgcgg atgttgagtg
     3901 caaatgacgt ttccagccac agaatgccgc cgttatgaaa ttcaatggtg ccaaacagcg
     3961 aatcttcgac gctgtaagtt gccggatccc actcgccaaa ttgaccacag ctcttttgcg
     4021 tgccgatctt ttgaaaacta tgcgcattca cgcttttcac cgccggaaag cccagcacat
     4081 acatcgcagc atccagcata tgaatgccga tgtcgatcag caggccacca ccctgcagtt
     4141 ctttattggt aaagacaccc cagccgggaa cgccgcagcg acgcagggcg cgggcggtgg
     4201 taacgtaaat ctctcccaaa acgccgttgg tcacctgttc acgcagctgt tgcgtatcga
     4261 gcgcaaacga tggtgaaagt cgtaggccag caccttgccc agtttgcgcg cggtatcgca
     4321 catttcccac gcctgttctg tcgtcatggc gggcggtttt tcgcacatca catggcagcc
     4381 tgcttccagt gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc cgacgctaac
     4441 cacatcaggt ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg atgcattccc
     4501 gtatttttct gccagcgcct gcgcctggga aaggcggctg tcacagacag ccaccagttc
     4561 cagatcgttg cgggtgcagt agtacgaagc atgaacctta tccgccacct ggcccgcgcc
     4621 tattatcgcg acccgcagcg gagagcttgt cattgcactt ttcacaaaga cctcttagca
     4681 ggtacgcaac caggccaacg aatcacggta cgcctgggca ggatcttctg cccggatacg
     4741 cccttcatac accacataac cctgatagtt atcggcgcgc agctgttcaa acagcgcgtg
     4801 gaaatcgagg gtgccactgc ccggctggta acgatggtta tcggcaatat gcacatgacc
     4861 gagcaagtca cggttgtcgt gcagcgcctg cgccaggtta tcttcttcga tgttcatgtg
     4921 atagaaatcg ccgataatct gcacatgttt aagattgttt tcgacgatgt aacgacgagc
     4981 atcggcgagg gtgttgatca tatgatcctg atagcggttt aacggttcga gatacaccac
     5041 ggttccggta cgcgcggcga cctgttccag tacgcgcagg gaatcactca ccagtttgcg
     5101 gtcgccgtcc aggctacgcg gcgaggtcat cggcggtaag cggaaggtaa acatgcccca
     5161 cgctgccgga aaccccccac ttctgccagc gcctcgagaa tgcgttcgat ctgctttaag
     5221 ccattaagac gacgctcttc gataaagtca ccaatccagc cgtcatagcc gccgcaggcg
     5281 gtggtcactg gtaaaccggt ttctttgatc gccgctttga cttcttcgat gttgttgacc
     5341 agcagtttgc cgtcaatctc aaaaccatcg aagcccatct ctttgatata acgaaatctc
     5401 tcgcggatat tttccggaaa gaatgcctga ttctgtgtgc cgattttcat tttttcattc
     5461 ctttgaatta aaacgtaacg cccattttga tgctctgttc cggatgctga tcgacatact
     5521 gcatgtagct ttccgggctg ttggcaaagg tcactaccgg gtcgattaaa tcttcgcaat
     5581 tgagataacc gttcatcagc agttcccagc aggtttcttc aatacgctta cggctccagc
     5641 gcggataatc cgggttcggt tcgctgcacg cgcgggagaa aacaattttg gcgttattga
     5701 aatgcgcttc gcgtccgagg ttaaaacctt cagcaaatgg tttggcaaac gcgacatagg
     5761 agatggtgcc gccgtaggcc aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc
     5821 tggtttcgat aatcacatca gccccctgct tgccggtcag cgttttgatc tctttgccta
     5881 cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc
     5941 gatgggcgat aggatcgacg ccaattacca ccgatgcgcc agcgcgtttc gccagttgga
     6001 tggcaatttg accgatcgcg ccaagcccta ccaccacgac aaaatcccct acgcgtacgt
     6061 tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacagacg gcgtttttcc
     6121 aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt cactgcgtta atgatgaccg
     6181 tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc
     6241 tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg ccaaactcaa
     6301 tgccgcgcgg cgcatctgcc ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt
     6361 catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc
     6421 cgaaacgcac gcggatcttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca
     6481 gcgcagcaac acgcggtgct gtggctacta actttttcat gtaaaactcc ttgtaaagaa
     6541 actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg
     6601 aaagcgcata cataatcacc accggcaatg cggtcaccag cgaggccgcc atcatccgtc
     6661 cccagatgta atcaggcgta ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc
     6721 tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgccacc ataaagcagt
     6781 agacgaacac cgatatcagc ccggacatcg ccaggggcac ggtgatacgg aagatgattt
     6841 gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcaggg atggtgtcga
     6901 agtaactttt cagcataaac acggcggtgg gcagggtttg cgtcaccatg gtaatgatca
     6961 gcgccatctc ggtgtcgtaa ataccaagcg cggtgatgat tttgaacaga ggcaccacca
     7021 gcaaaatgcc agagaacata tacaccgtgt aaaagctggc gttgatggtc atccggcctt
     7081 taaagcgcag gcgggaaagc gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg
     7141 aggaaaccac cgacaccacc atgctgttac ggaagtagtc gacaaacggg aaaatcatcg
     7201 ggttaaaaat gtcgacgtaa tgctccagcg tccactgttg cggcagcagc gtaggatgca
     7261 gcgatatcgc ctctttcgcg cttttgaacg aggtcatcag catcacaaaa aacggaaaca
     7321 gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt
     7381 tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg
     7441 atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt
     7501 ttgtagagat agacaccaag aatatcgacc ttggttgtca gcagataaac atcagcgaac
     7561 atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaggaccgg cataattgcg
     7621 ggcagcgtga cgatgcgaaa ccgctgccag gcattagcgc catccatctc tgccgcttcg
     7681 tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag
     7741 tagcgccaga tggcgaacag caccaccagc acaaagctac tgcccggatt atcaaaccac
     7801 agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag
     7861 ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt tacgtaggag
     7921 aggatcacca gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc
     7981 acggccagcc cgagcacggt gctgcccgcc accaccagcg ccgtatacca gaccgtcatc
     8041 cacagcgagt gccagaagcc gggatcggag agaatgcgca catagttact caccccaaca
     8101 aacgttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc gatattcgac
     8161 accatcggcc acgccaccag accgcccagc agtaataagc tgggcgcgag aagcagcagc
     8221 gcaaacggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc ctgtcctggg
     8281 tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc gtgccaggga
     8341 gatcggcttt acccacggtg acgttatgca ccatggagct caccacacca gaacccgtca
     8401 catcgcccat gcgggtaaag tttttatccc ctactgcgcc aaagacctga atatttggca
     8461 gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc
     8521 aggtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc gacatcatca
     8581 cccagtcggc aatgttgtcc gcctgctcca taaaggtgac aaatttttct gctgcttcgg
     8641 tcttttgtcc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct
     8701 cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga
     8761 tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca
     8821 tgatgtcgtt agaacccggc atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct
     8881 gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg ttggcctagt
     8941 tggataacgc aaactgggag aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg
     9001 caataccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt
     9061 gccagttttt cggctcctcc agccccgctt ttgccagcac atctttgcga taccagatgt
     9121 cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa
     9181 tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg
     9241 ctttgcgatc gataagctgc tctttatcca taactttggc gtagtcatgg ctggtttcga
     9301 tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac tttggtgtta tgagcgtctt
     9361 cttccacggg cacttgttta acggtgatac ctgggttttc tttttcaaaa cgggcaatca
     9421 atttgctgat aactgcctga cgctcctgct ccaccgaaga atgcataaat tcgatggata
     9481 catgggtttt attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata
     9541 acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc
     9601 caggggcgca gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca
     9661 tgcagatcag taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga
     9721 caattaccat cagcgttact tctttgaata cgcattacgg atgaattaac cgtatcgatg
     9781 gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac
     9841 aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg tgttatctct
     9901 tcactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg
     9961 taatcattac gcgagccaag aatactttga atatatatcg cgggtacacc ggggaaactt
    10021 aacaaaatcg catgggcaag gataaacctg gtgcaacgtt cttcatcgct actctcgcgg
    10081 cggcttaacg cattcatata ggtgacattc atctcatagg gactgcgcgt gccgtcggga
    10141 ttatttttcc agtttact
//
LOCUS       DBKELA010000126        10071 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.126, whole genome shotgun sequence.
ACCESSION   DBKELA010000126 DBKELA010000000
VERSION     DBKELA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.126"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..168)
                     /locus_tag="ACUQR0_003480"
     CDS             complement(<1..168)
                     /locus_tag="ACUQR0_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HHV9110366.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKK"
     gene            complement(371..838)
                     /locus_tag="ACUQR0_003481"
     CDS             complement(371..838)
                     /locus_tag="ACUQR0_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HHV9110367.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(835..1368)
                     /locus_tag="ACUQR0_003482"
     CDS             complement(835..1368)
                     /locus_tag="ACUQR0_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HHV9110368.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1434..2063)
                     /locus_tag="ACUQR0_003483"
     CDS             complement(1434..2063)
                     /locus_tag="ACUQR0_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110369.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2067..2282)
                     /locus_tag="ACUQR0_003484"
     CDS             complement(2067..2282)
                     /locus_tag="ACUQR0_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HHV9110370.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2575..2649)
                     /locus_tag="ACUQR0_003485"
     tRNA            complement(2575..2649)
                     /locus_tag="ACUQR0_003485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2614..2616),aa:Gly,seq:tcc)
     gene            complement(2653..2728)
                     /locus_tag="ACUQR0_003486"
     tRNA            complement(2653..2728)
                     /locus_tag="ACUQR0_003486"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2693..2695),aa:Thr,seq:tgt)
     gene            complement(2731..2807)
                     /locus_tag="ACUQR0_003487"
     tRNA            complement(2731..2807)
                     /locus_tag="ACUQR0_003487"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2771..2773),aa:Arg,seq:tct)
     gene            complement(2809..2885)
                     /locus_tag="ACUQR0_003488"
     tRNA            complement(2809..2885)
                     /locus_tag="ACUQR0_003488"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2849..2851),aa:Ile,seq:cat)
     gene            complement(3085..3465)
                     /locus_tag="ACUQR0_003489"
     CDS             complement(3085..3465)
                     /locus_tag="ACUQR0_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HHV9110371.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3458..3658)
                     /locus_tag="ACUQR0_003490"
     CDS             complement(3458..3658)
                     /locus_tag="ACUQR0_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HHV9110372.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3753..4010)
                     /locus_tag="ACUQR0_003491"
     CDS             complement(3753..4010)
                     /locus_tag="ACUQR0_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110373.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(4007..5398)
                     /locus_tag="ACUQR0_003492"
     CDS             complement(4007..5398)
                     /locus_tag="ACUQR0_003492"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HHV9110374.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(5395..6273)
                     /locus_tag="ACUQR0_003493"
     CDS             complement(5395..6273)
                     /locus_tag="ACUQR0_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9110375.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(6284..7192)
                     /locus_tag="ACUQR0_003494"
     CDS             complement(6284..7192)
                     /locus_tag="ACUQR0_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HHV9110376.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7179..7412)
                     /locus_tag="ACUQR0_003495"
     CDS             complement(7179..7412)
                     /locus_tag="ACUQR0_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110377.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7409..8425)
                     /locus_tag="ACUQR0_003496"
                     /pseudo
     CDS             complement(7409..8425)
                     /locus_tag="ACUQR0_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            8390..8620
                     /locus_tag="ACUQR0_003497"
     CDS             8390..8620
                     /locus_tag="ACUQR0_003497"
                     /inference="COORDINATES: protein motif:HMM:NF017757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHV9110378.1"
                     /translation="MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRI
                     IMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8610..8918
                     /locus_tag="ACUQR0_003498"
     CDS             8610..8918
                     /locus_tag="ACUQR0_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9110379.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8960..9136)
                     /locus_tag="ACUQR0_003499"
     CDS             complement(8960..9136)
                     /locus_tag="ACUQR0_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HHV9110380.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(9133..9543)
                     /locus_tag="ACUQR0_003500"
     CDS             complement(9133..9543)
                     /locus_tag="ACUQR0_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HHV9110381.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(9540..9818)
                     /locus_tag="ACUQR0_003501"
     CDS             complement(9540..9818)
                     /locus_tag="ACUQR0_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110382.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
BASE COUNT         2434 a         2702 c         2309 g         2626 t
ORIGIN      
        1 tttcttcatg aaactgcggt gttcgagttt gcgatatttc ttacagggga acgcaagccc
       61 ctccgcctcc gctttggatt tccgatcagc attaatgtaa tccaccatct caagactgct
      121 catggtcggg tattcgccag aaggaagaac ggttaatgat ttgttcatgt cggtatttcc
      181 tttagaaagt tgagcctgtt cgcacagaaa agccgtcccc gagaggtcct cacctatacg
      241 gcagttctca ggctcagctt tctgaaagac tcgggattgt tacgcgctgc gatgcgcggt
      301 ttactgcgga catataaaag ccccgcaagt gcgaggctca ttaaatggac tttgtgattt
      361 gcaaaaaaat ttatttcagg cactgagtcc tgatgtactc ctgaagcatt ctcaatgctg
      421 tctggtcgct gatgattccg tcccggatac cgagaacgtt tcgtccagca actggagaga
      481 gttcgacggt ggcatcattg cccatgccgg aggcgctgga ggtttcggct gaggatggca
      541 cagggcattt tcctttgacg agcacccgac caccattatc aagcttgcgc cgaagagcat
      601 cattttcagc tttcgcatca gctaactcct tcgtgtattt agcatcgagt gcatcagcat
      661 cacgctggcg ctgctgcatg tcagtaatgg ttgcgttcgc cagcttcagt tctccggcgt
      721 ttttgtcgcg ctgctctttg taggtaatgg cgttatcacg gtaatgatta acagcccatg
      781 acaggcagac gatgatgcag ataaccagag cggagataat cgcggtgact ctgctcattg
      841 atctatcccc caacaggcta atgcgctttc ctggtcacga cgaataacct gtccatagca
      901 gttatttgaa cgtatgcggc aatcgcgccc aacatctttt atccaccagc gaatcgcctc
      961 gcatgcgccc ttacgatcac cggcattcag ccgcttataa aacgtcgacg ggaaacactt
     1021 accggggcca atgttatagg ggcaaaatga cgctataccc gctttctggg gttcggtcag
     1081 aggtacttta atattgcgct ccacccatgc cagcgcctta tcacgttcaa tagcgttaac
     1141 ctggtcacat ttttccttcg acagcttcat tcccggtatg acaggcttac catccaccat
     1201 tgtggcacca cgacagatgg tccatatgcc agaaccatca cggtatgccg ttgtgtgatt
     1261 accctctttt tcgttcagaa actggtcaag tatctgggga gcagacgcgc ctgcggcaat
     1321 cagcgccaga acagcagccg acaggccgta tttgattttt gcgctcatgg atatttatca
     1381 ggatttatcg gcaacaaata acgagccaac ttatatacgc cttttaagat aagtcagccc
     1441 cggctggatc cagtcatcag gctctttctt aaagggggag tattgaaatc acgaagaaga
     1501 gcctcccgca ccgcagcatc catgtctgca ccactggcaa gtgcttcaat ctctgccgcc
     1561 acctgcaaat accccatgca acggccaatg cgtttcataa ctctggtgtt ttcgtctttc
     1621 aggcatctga tagcgagatt aataattttc aggcagtgat gatgcgatgc ccggacaggt
     1681 tttccgttct cataaacgac gaactcaccc tcttcacagg aaaccctgaa aggccgatgg
     1741 ggttcctggt ctttatccgg tgcagacgca ataaaatcac ttttatgctc ttcctctccg
     1801 gcaccgttaa ccgtaatgac gtactcaggc cgcaggtaag ccgcctcttt ttctgacaat
     1861 gcggccggta aaaacttttc ctgcaaaaac ttcataaaag tctcgtgagc gactttgtgg
     1921 cagtaatcgt aaatcgccgc ataatgctca tcgcggcgtt tgttttcatc ttcagaactc
     1981 accaacgccg acaatttttt attaagctca gtgatttcat tttccaggtg attgaatcgc
     2041 tgattcattt cttcatgctt cattatttac tctccccgtg cagccttacg cttgtcttct
     2101 cttattttga aatacagatt cgtcagataa gtcagaagcc ccagaagcag acttcccagc
     2161 acaccaatcg cagcccactg tgatggcctg acctgatcca accactgcaa aaaccagtag
     2221 ccggcactgc cggcggaggt gccgtaggca atgcccgttg aaattttgtc catggatttc
     2281 atagcctcac ctccgcaaat aacggatggc gtagttttac actgagaaat gaaagggatt
     2341 tgaaaagaaa aaaacgcaaa agcgggcgaa acgatatata cagtaaggaa agcactctat
     2401 ccaacaaacc acccacagtt aatcggaata aaagcagagt gcttatgaat gatcgcacgc
     2461 ccgaaggtta gtatttctgc acagcaattt tgcaaaaaaa ggcgatcatt cataacttaa
     2521 acgtctttca gtcactccgg gatttcccat catcgcagac tgaaagactc tgactggagc
     2581 gggcagcggg aatcgaaccc gcatcatcag cttggaaggc tgaggtaata gccattatac
     2641 gatgcccgca tatggtgccg actaccggaa tcgaactggt gacctactga ttacaagtca
     2701 gttgctctgc ctgctgagct aagtcggcgc tggcccacca ccgaggactc gaacctcgca
     2761 ccgtcaactt agaaggttga tgctctatcc ggatgagcta gtggtggttg gtggcccttg
     2821 ctggacttga accagcgacc tggcgattat gagtcgctcg ctctgaccaa ctgagctaaa
     2881 gggccggagg cagaataata accatatgtc atcacatctg caaactcatc tgaccaccag
     2941 cgtgtttaac gtcctgtacc gtttttcagg cataaaaaac ccgcccggag gcgggtttaa
     3001 gctgtgtggc gaagcaacca ctcttaacag aatatcccgt tttttacgta cgtaaaatat
     3061 tttctagaaa gtcgcccctt accatcaggg atgttcaata tatttgtcca tttctaaccg
     3121 gacacccaac atcatcaaca ttccttccac caccccctcg gctttttgta gcttctttcc
     3181 tatataacca tctgagcatc catgcttccg cgccagcgtc atgaatgtca tcccaaacac
     3241 gtagtagtca accagcaagt catgtagatc gttgttgttc cggttaaggc gggccataca
     3301 cccacatata accatcgcgt catcgtcaca gcattgcggg cgggatttta cttttgaagg
     3361 gattaatccc ttaaaaccgg cagcaatgga cgaccaggtc acatcctcat gattattagc
     3421 cacccacgcg ccccagcgct caagaacctg ctgaatatca cgcatcactg tctttacccc
     3481 tgtcccatcc acgatgaaca atcagaacac catcaacaat ggcgtgccct ttgccctctt
     3541 tatcttcagc atattttctt accgtggcgc gattacagtt cagcattcgt gaaacttcgg
     3601 tcatattacc tcgtgtctgg ataagcagtt ccggtatcgt ttgaattttg acgctcatca
     3661 aatactctcc ggttcggtga tttttatccc gggctttcca ccagggacat aatgatcgcg
     3721 cacaagccag ggcaaaacaa actcatggcg catcagcgca gcgcctcctg caccagtttt
     3781 tcaaactttc cgactctggt ttccagctct gccacacagt ccaccagctc atctactgct
     3841 ttctgtgcgc gatgcttcgc ctgcatcagt tcccgaagcg cgggtaccat atctttacgg
     3901 atagcatctt ttgttatgcc cgttttttcg agttgttcag catgacgcag catttcctgc
     3961 gcgtgtttac gcaattgttc aggggtaaaa gtcattgtct ggttgttcaa aagaaacgct
     4021 ccatcttact gctgtcagtt cgtttattac tgtatctgcg cggattgccg ggcttcatgg
     4081 gagtggaaag cacccgtgca ctttcctggt ccacaggcag aaaatgtccg ttataaaaac
     4141 gccggtaaat cgttcccaga gaaccgttac gttgtttcgt gatattgatt tctgcgatgc
     4201 ccctggcctg cgtatccggg ttgtacactt catccctgta aagcatcaga atgatgtctg
     4261 catccgcctc tatttctccg gaatttttca ggtctgagtt catgggacgt ttattgggtc
     4321 tggactccac accgcgggag agctggctca gcgcaatcaa cggaaaacca ccggattttg
     4381 ccaggccttt aagccccttt gagatttcac ccacggcaag gtcatgacgc cccgtggttc
     4441 gggtttttat cagcccgaga taatcaacca ccaccagcgc cgtttccgga tatttaatca
     4501 ggtggtgttt cgttgttgcg catatctcat caatggccag gttcgcctgg tccaccatcc
     4561 agatattgcg cccggtcatc cgccccaccc cttgtgagaa acgcgtccag tcttcatctt
     4621 caaagtgagc cacagatttc aggcgtgata ctggcatccc tccagccgca gacaccatac
     4681 gttcaccaat ctggatgttc gccatctcca tggtgaacag aagcacacca tgcccctgct
     4741 cagtcacctt gtcgatgatg tccagcgcaa gttcggtttt ccccatcgaa ggacgggcgg
     4801 caatgaatac caggtctccg ggctccatac cgcctgtttt tgcgtccagt tcatcaatac
     4861 cggtcatcaa cgtcctggat ttctccagcc cctgattccg gcattcaaca cgctcaacca
     4921 cttccggaag cacatcatca atatgtaccg gctgaatgac gccctttcct gtcgacagtg
     4981 tgaccatcat gttctgcgca tccttcaggg catcttcagc tgcttcacag gtatgcgcat
     5041 cacgtaattt ctgcagcgcc tcattcagtg ttttttctgc atcgcgcagt gcggcattgc
     5101 gccgcaacgc tgcaacatag tgctccagtg aagacttcac ccaggtttta cgcccggtat
     5161 cagtaatcac cggggcaagt tccggcatct cattacacaa cagcacgggg tcaatcactc
     5221 ctgaaacacg ggcctgtctg cagatgcctg tatagatatc ctgatacgct cgtacagaaa
     5281 aaacgtccgc tggcagtgtg gccagaatat ccatcacttc aggatctgct ccgcgcagaa
     5341 agaacgcgcc gatgacagcg ccttcaaggt catcgttacg ccatactgga gttgtcatgc
     5401 agccacacct ctgatacgag aacggtaact gggccagtta aacgacaacc agttgcgtcc
     5461 cccgtctgtg atcctgtcgg caattcgggg actgatgaac gcccacaact cttccggtga
     5521 gaggttactg ataagaatgg tgggcaagat actttcgtac ctggcgttga taatttcctg
     5581 caaaatagcc atttcagccg cgctgccaaa ctgaacgcca acttcgtcga tgatcagcaa
     5641 atccattgac gcataacgct caataacttc atccgctgtt ttttcgctgt cattccgcca
     5701 gcaatttttc acagcacggg taaggcgcat cacgtcggtg atctccacac tggccagata
     5761 gttacggatg atgtgttttg ccatagccac agccagatga ttttttccgg taccacaact
     5821 gccggtcata acaagactgg taccgttctc cagcatatct ggccagttct ccgcatagcg
     5881 gcgacaggcc gcaagatttc tggctgcgcc aggattaacc tccagataat tatcaaactc
     5941 gcagtcccga aaacgcaggg caattccggc gttatcagtc agctcttccg ccttgatgga
     6001 cgacagctcc atggtcaaat cgttggcctc agcgattaag cagtcagggc agcatgaaat
     6061 ttcttctctg tcctcgccat tacgatcgct ccacaccagt atatgcgtgt gatattcgcc
     6121 atgtttttcg caatatccgc gaccttcacg catcaggcag gaacgataag gccatggctt
     6181 ttcgcccttc tgagcaaatg caatctctgc ccgtaactca tccattcgcg cctgtagtct
     6241 tttttgttgt tcacgcaggt taaacgtcat catcgctgtc acctcagaat gtcagtttgt
     6301 cactggattt accgaatttg tcagacatgg ctcccaggcc agccaggaca tcgacctgtc
     6361 gctgtcgccc acctctggga gcggctggct gttgccagaa gtcttcgaag tgacgatcgg
     6421 gtccaaagaa cgtcgcagcc tgcttcacga actgtgtgcc ggtatttcct gtggcacgta
     6481 cccaggcagc atagcgtttc acgccatcaa gcatggcttc aggttttatt ccctccctga
     6541 gccgggcttt ccaggctttg aaggctgcgg attttgagtt accaccagca cgttttggat
     6601 attcctgcca ggcctgttca aattccggtg aatattcctg tcgggaagaa cgcgctggtg
     6661 cagacgcgtc agcggatgcg ccaatagtgt ttttactctc tgtagtattc tctgaagtaa
     6721 tctctgttgt attctctgta agatcgaaat tggttttccc ttctccgcgg cgaggggttt
     6781 cccgtgtccg cggtgaaggc tttccctcct ccgcgaaatt gggttttaca gtttcccgaa
     6841 aacgggtttc cccatttcgg gaaaactgat tgttttcatt gataatttca ttaaggcgct
     6901 cacaatctat acggtagaac attttgtgct caagacgctt gttggtttca accaaaatgc
     6961 ctctggacac aagatgctta cgcgctacag cctgttgttc aaatgtaagt ccggtttcgt
     7021 gttgtatctc ttcacgcgtt ttatgtacgc cttccgctgc atgtgcttta tcctgccagt
     7081 aaaaaatctg accaaagaaa ataacagcgt gcggacttcc catgtattta acgagcccag
     7141 ggtaataagc aaccggatgt ccaaaatcga gcagaagatc agacggacgc atagccacct
     7201 cccaggcgtt taaacatttt tccgggctga aacgccacca gcggataact cagggtatga
     7261 gtacgtccct gaacctggca gacaaccttc tggctttcgg tactgaccag gcaaacccgc
     7321 agaacgtagc cgttgctggt ggtgaaccac tgccccacac gggggcaatg gttgtatcgg
     7381 tgatacagga aattaacgat gtggcggatc atgggcgcac ctccttgtca gaaccattca
     7441 gcctggaatc aacaagtgca gcaccaaaaa cagcatcacc tacacggtcg tacagtttgc
     7501 cagccagcgg agattcaacg gccttaagca ttgggtaaag ctggcttgtc cagatttgat
     7561 ggatttcacg caaatgcagg tatacgcctc tggcgttttg tgcgacagat gacatgtcag
     7621 ccgcaccagc tcctgataag ctcctctcca tctggttaaa ggcattgatg tatgcctctt
     7681 tgaaccgggc ggcacgttta ccagtgaagc ccatagcaag gaaggcgaag ccgccgcggg
     7741 taatttggta gcagggaagt ttgcgagtac cgccgttggg ctggcgtacc aaaattgatg
     7801 tctccgcaaa attgcgggca caaaactctg gagaacaatc caaaatgcgg atctttttca
     7861 gaacatcgtc atgacgttta gagaagaagt tggcaacagc cagggatgaa gtaacagcct
     7921 aaccatcaac gatggcaatt tcaggttgag agagggttgg gagagtagtc atggtgacag
     7981 cccctatgtt gaattcaaag aactcaccac atgggacgcc aatcacagag gtggtgagac
     8041 gtacagggtt ggcgtaaccg gtcaacatag aacccggcgc atcttgcgat gtccctgcac
     8101 gccccaccat aatttgggcg tagcaatgct catgacacga aaaaaccgca tgagcgcggt
     8161 tatgctctat attgaatttc aggacgccaa tcccggcccc cgctttataa ggtgcagaga
     8221 cagtgtaacg tcccgaaatt gcagaatcaa tatattgtgc agatataacg attttgttat
     8281 gttttgctgt atgaactaca ctatcgaata ctacagtgaa gaggtccgac tggaggtcga
     8341 tcagcttcca ttgagtatgc gtgccagata ccagcatctt gttgaacgca tgaaggtata
     8401 cggcagcaat ctcggagaac ctcacaccag cgcctttggt gacgggcttt tcgaactcag
     8461 aattaaaggc aacgatggga tcgcgcgtgt tttttactgc actctgacag ggaaacgcat
     8521 catcatgctg catagttttg taaagaaagc gcagaaaaca ccgccagccg aacgcaagaa
     8581 agctgaaacc agaatgaagg aggttaagca tgactggtaa acgcactctc cccaccatga
     8641 cacacgacga aatggcagcc aaatggatgg aagacccggc ttttaaagca gaatacgacg
     8701 ctatcgctga cgaattcgca ctgcttgatg aaatgctggc agcacgcaaa gaagctggct
     8761 taactcaggc tgaagttgcc gagcgaatgg gaacaaaagc gaccgcgatc accagaatgg
     8821 aaagtaatct cgcatcaggt atcagcggcc catcatttgc cacactaaaa aaattcgccc
     8881 gcgctactgg aaaaaaactc cagatccgct tcgtttaacc acaccgcgcc gtcattctgg
     8941 cggcgcaccg gataaatagt catttcctcg cacgatgtct tagccaccgg atatcccaca
     9001 ggtgagtcgt gtagttgaag gtttttacgt cagattcttt tgggattggc ttgcgtttat
     9061 ttctggagcg tttcgttgga aggtatttgc agttttcgca gatgatgtcg gtgaaacttc
     9121 gtcgatgtcg cctcattcat acctcctgtc ggtaaatctg acaccctgac caatagccca
     9181 ggctgttgtg tactcgatca gacttgccat gcgcttcacg ctcatctgcg cgctgctttc
     9241 gcgaatgttg acgcattctc cttcaaggcc gggcaaaaca tcagcttcct gttttgttgc
     9301 cactgcatga ccgctgatca acaaaacctt ccattgttct ggttttaacc atttgccgca
     9361 ccattgaacc tgacgagcga tatccgccag catcgcgtga aattttgcgt tctggtcaag
     9421 atttcgcttg tagtcagtaa tgcggatggt gactggcttg tctttatcga gaggagttga
     9481 gaggatggca tttattgcgg cttgctgttg ttgcttagtt cggaggaaga ttgtttgctt
     9541 catcgaaaat tcttctcttt aattccagcg gctctgatag ctttcattac tgcaattacc
     9601 gttttgtcct tcccatcctc atgccccatc gcataagcac cttcttcacc atctttccaa
     9661 aagtcgtcat tcgattcggg ccagtcgata tccagttcaa tagctgctcg cgatgcctgc
     9721 catatcaccc aggcaaactc ttttaattca tcgtctcctg tgaactggct tttgtctttt
     9781 gaccaccagt tttcaaactg tcggtagcta tcgttcactt ccctctcccc caaataaaaa
     9841 ggcctgcgat taccagcagg cctgctatta gctcagtgat gtagatggtc atttaatact
     9901 ccgtcacgtt ttcctgtcgc cacgcctcgt catattccga tttcggcata ttggcgatgt
     9961 agctatatgg cgatcctgat tcaagttgca ggaactggtg cgattgctcg tcaaggaaca
    10021 acggaacacc accttcccaa ccttcgccgt tacgttgttt ttcaagcatc a
//
LOCUS       DBKELA010000127         9951 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.127, whole genome shotgun sequence.
ACCESSION   DBKELA010000127 DBKELA010000000
VERSION     DBKELA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.127"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..66)
                     /locus_tag="ACUQR0_003502"
     CDS             complement(<1..66)
                     /locus_tag="ACUQR0_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110383.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRI"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="ACUQR0_003503"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="ACUQR0_003503"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HHV9110384.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     STFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="ACUQR0_003504"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="ACUQR0_003504"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HHV9110385.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="ACUQR0_003505"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="ACUQR0_003505"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HHV9110386.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="ACUQR0_003506"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="ACUQR0_003506"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HHV9110387.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="ACUQR0_003507"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="ACUQR0_003507"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HHV9110388.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="ACUQR0_003508"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="ACUQR0_003508"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HHV9110389.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="ACUQR0_003509"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="ACUQR0_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="ACUQR0_003510"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="ACUQR0_003510"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HHV9110390.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="ACUQR0_003511"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="ACUQR0_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HHV9110391.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8881..8970
                     /gene="yldA"
                     /locus_tag="ACUQR0_003512"
     CDS             8881..8970
                     /gene="yldA"
                     /locus_tag="ACUQR0_003512"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HHV9110392.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8974..>9951)
                     /locus_tag="ACUQR0_003513"
     CDS             complement(8974..>9951)
                     /locus_tag="ACUQR0_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HHV9110393.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2505 g         2420 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgct ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DBKELA010000128         9906 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.128, whole genome shotgun sequence.
ACCESSION   DBKELA010000128 DBKELA010000000
VERSION     DBKELA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.128"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..79
                     /locus_tag="ACUQR0_003514"
     CDS             <1..79
                     /locus_tag="ACUQR0_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HHV9110394.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(161..901)
                     /gene="pflA"
                     /locus_tag="ACUQR0_003515"
     CDS             complement(161..901)
                     /gene="pflA"
                     /locus_tag="ACUQR0_003515"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HHV9110395.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1093..3375)
                     /gene="pflB"
                     /locus_tag="ACUQR0_003516"
     CDS             complement(1093..3375)
                     /gene="pflB"
                     /locus_tag="ACUQR0_003516"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HHV9110396.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3430..4287)
                     /gene="focA"
                     /locus_tag="ACUQR0_003517"
     CDS             complement(3430..4287)
                     /gene="focA"
                     /locus_tag="ACUQR0_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HHV9110397.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4693..6453)
                     /gene="ycaO"
                     /locus_tag="ACUQR0_003518"
     CDS             complement(4693..6453)
                     /gene="ycaO"
                     /locus_tag="ACUQR0_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HHV9110398.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6583..7209
                     /locus_tag="ACUQR0_003519"
                     /pseudo
     CDS             6583..7209
                     /locus_tag="ACUQR0_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7408..8496
                     /gene="serC"
                     /locus_tag="ACUQR0_003520"
     CDS             7408..8496
                     /gene="serC"
                     /locus_tag="ACUQR0_003520"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HHV9110399.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8567..9850
                     /gene="aroA"
                     /locus_tag="ACUQR0_003521"
     CDS             8567..9850
                     /gene="aroA"
                     /locus_tag="ACUQR0_003521"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HHV9110400.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2368 c         2482 g         2535 t
ORIGIN      
        1 attcgatggg gaagaacttc acgcgagcta ttatcagtcg ttattatctc aggtacggga
       61 tatggtagat gcactataag atgtgttaaa aacgctgtag cagaatgaag cgcggaataa
      121 aaaagcggca actcaataaa gttgccgctt tacgaggaaa ttagaacatt accttatgac
      181 cgtactgctc aagaatgcct ttcacgcgtt ccatggtctc tttcttcggt ggtttaacac
      241 catcgagttt gtattcttca cccatcgcca cccatttgtg tttgcccagt tcgtggtagg
      301 ggagaagttc gattttctca acgttaccca tatcacgggt aaattcaccg aggcgatgtg
      361 ccgaatcgtc atcgtcagac cagcctggga caacaacgta gcggatccac accttcacat
      421 ttttgttcgc cagatattta gcgaactcca gcgtgcggtg gttggaaact ccaaccagat
      481 tttggtggat ctcgtcgttc atctgtttga gatcgagcat taccaggtcg gttacttcca
      541 gcagttcatc aatcaccgga tcgtaacgac gaacaaaacc gttggtatcc agacaggtat
      601 gaatgccttc ttttttacag gcgcggaacc agtcacgaac aaactcagct tgcaggattg
      661 cttcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca
      721 ccacttcctt catcaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt
      781 cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa
      841 tacctgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca
      901 ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct
      961 acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg
     1021 tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc
     1081 agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc
     1141 tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata
     1201 tttttccggg ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag
     1261 gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca gaccagccag
     1321 gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta
     1381 ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca gagacgctac
     1441 agcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc
     1501 acgacgaccg tctggggtgt taccagtttt cttaccatac acaacgttag aagtgatggt
     1561 cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat
     1621 gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcattgt taccaaactg
     1681 cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt
     1741 aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc
     1801 acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct
     1861 gtacttgtcg tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagccagcca
     1921 gtccatgaag tgatccatgc gctccatcac ttcatcgtag ctcaggagat cgcctttgat
     1981 cggttcagac ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc
     2041 gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac
     2101 gatcatcggg cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt ccggacgcat
     2161 caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag cggcgaattt
     2221 cttgaagttc agcggcagtt tttcagacca cagaatggtc atgttcggtt ccggagacgg
     2281 ccccatagtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc
     2341 atcgaggccc ataccaccga tagattcggt tgcccagatt gggtcgccag agaacagttc
     2401 atcgtattcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca ggtggtcaac
     2461 catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt cgatgtacac
     2521 atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag acttaacagc
     2581 agccaggtag ccgaagtaag tccactggat agcttcctga gcgttggtag ccggaccaga
     2641 gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc ggtgctgttc
     2701 agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt ccagatcagc
     2761 ctgtagagag gtgaactgag cgtatttgtc tttcatcagg tagtcgatac cgtacagcgc
     2821 aacgcgacgg tagtcaccga tgatacggcc acggccataa gcatctggca gaccggtcag
     2881 aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt
     2941 gtgagtttta cggtattcag tgaagatttt tttgatcatc gggtccagtt cgcggttgta
     3001 cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag cacgtttcag
     3061 cggagcttca gtctgcagac caacaacttt ttccaacgct ttattgatgt agccagcgtc
     3121 gtgagaggtg atggtggaag caacagcggt gtcaaaatca actggcgcgt gagtgcggtt
     3181 ttccagttta acaccttcca ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc
     3241 agccaggaag gactcgtcac cctcgtacgg cgtgtagttt ttctgaatga agtcacggac
     3301 gtttacttca ttctgccagt cacctttggt aaaaccttcc caggctgtgg ctaacttttc
     3361 attaagctcg gacatgtaac acctaccttc ttaagtggat tttttattta ctgcgtactt
     3421 cgacaaccat taatggtgat cgttttcacg caggtaaatg acccagtatg tcaacccaac
     3481 caacaaacca ccgccgataa tattaccgat cgtaaccggg atcaggttat cagtgatgaa
     3541 gttcatcacg gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg tccagaattc
     3601 cggagatgcg aagtcgcgga ttacaatacc catcgggatc ataaacatgt ttgcgatact
     3661 gtgctcaaaa ccgctggcaa caaacatcgc gaccggcagc accataatga acgctttgtc
     3721 catcaggctg cggccagaat aactcatcca tactgccaga cataccatca ggtttgccag
     3781 gataccaaga cagacggcct caataaaagt atggtgcact ttgtggtcgg cggtttgtag
     3841 gacgtttagt ccccattgac catttgcggt catatactcg ccggaaagcc acattaaaag
     3901 tacaaacagc agtgcgccga ccaggttgcc aaaatagaca tttagccagt ttttagccaa
     3961 ctgacaccag gtgatgcgcc cactcgcctt agcaacaaca atcaacactg tggaagtaaa
     4021 gagatcggct ccgcagacaa cacaaagaat cagccccaga gagaagcaaa tgccgccaac
     4081 cagttttgcc atgccgaagg gcattgtgcc tgtgccagtg gttgctgtga tatagaagac
     4141 gaatgcgatt gagatgaaaa caccggcggt aatcgccaga tagaaagtct taagcggatg
     4201 tttcgttgct ttatagacac ccgcctcttc ggccactttg gccattgcag caggaagtaa
     4261 aagatcaaaa gggttgtcag ctttcacact aactctctct ttattaagtc ggcgacgaga
     4321 tactaacaaa gcattataga tgagaaattg atatagatca tatctcgcct ggcttatagg
     4381 cccgtaactc gcatggtttt tatgcaaata cggagtaact atttgattat ccaaataaaa
     4441 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4501 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4561 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4621 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4681 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4741 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4801 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4861 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4921 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4981 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     5041 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     5101 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa tcttcttcat ccagttgctc
     5161 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     5221 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     5281 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     5341 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     5401 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     5461 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5521 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5581 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5641 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5701 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5761 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5821 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5881 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5941 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     6001 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     6061 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     6121 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     6181 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     6241 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     6301 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     6361 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     6421 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6481 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6541 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6601 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6661 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6721 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6781 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6841 tataccgcct ggtaatatga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6901 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6961 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     7021 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     7081 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     7141 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     7201 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     7261 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     7321 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     7381 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     7441 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     7501 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     7561 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     7621 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     7681 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     7741 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     7801 aagccaatgc gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat
     7861 gaaaccatcg acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc
     7921 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     7981 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     8041 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     8101 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     8161 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     8221 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     8281 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     8341 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc
     8401 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     8461 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     8521 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     8581 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     8641 taaccgcgct ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct
     8701 ggatagcgac gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac
     8761 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     8821 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     8881 tctttgtctg gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     8941 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     9001 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     9061 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     9121 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     9181 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     9241 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     9301 ggcttcttac ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     9361 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     9421 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     9481 tatggatatg aaccatattc ctgatgcggc gatgaccatt gccacggcgg cgttatttgc
     9541 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     9601 gtttgcgatg gcaacagaac tgcgtaaagt cggcgcggaa gtggaagagg ggcacgatta
     9661 cattcgtatc actcctccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     9721 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     9781 tcccaaatgc acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca
     9841 ggcagcctga atgaacaacg ggcaataaat agccaaatct ttctttatca aaacgtcggc
     9901 acattg
//
LOCUS       DBKELA010000129         9863 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.129, whole genome shotgun sequence.
ACCESSION   DBKELA010000129 DBKELA010000000
VERSION     DBKELA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.129"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(131..1111)
                     /gene="ygfZ"
                     /locus_tag="ACUQR0_003522"
     CDS             complement(131..1111)
                     /gene="ygfZ"
                     /locus_tag="ACUQR0_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HHV9110401.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1354..1620
                     /gene="sdhE"
                     /locus_tag="ACUQR0_003523"
     CDS             1354..1620
                     /gene="sdhE"
                     /locus_tag="ACUQR0_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HHV9110402.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            1601..2008
                     /locus_tag="ACUQR0_003524"
     CDS             1601..2008
                     /locus_tag="ACUQR0_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HHV9110403.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2048..2569)
                     /gene="fldB"
                     /locus_tag="ACUQR0_003525"
     CDS             complement(2048..2569)
                     /gene="fldB"
                     /locus_tag="ACUQR0_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HHV9110404.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2681..3577
                     /gene="xerD"
                     /locus_tag="ACUQR0_003526"
     CDS             2681..3577
                     /gene="xerD"
                     /locus_tag="ACUQR0_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HHV9110405.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3602..4312
                     /gene="dsbC"
                     /locus_tag="ACUQR0_003527"
     CDS             3602..4312
                     /gene="dsbC"
                     /locus_tag="ACUQR0_003527"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HHV9110406.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            4318..6051
                     /gene="recJ"
                     /locus_tag="ACUQR0_003528"
     CDS             4318..6051
                     /gene="recJ"
                     /locus_tag="ACUQR0_003528"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HHV9110407.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6142..7240
                     /gene="prfB"
                     /locus_tag="ACUQR0_003529"
     CDS             join(6142..6216,6218..7240)
                     /gene="prfB"
                     /locus_tag="ACUQR0_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HHV9110408.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7250..8767
                     /gene="lysS"
                     /locus_tag="ACUQR0_003530"
     CDS             7250..8767
                     /gene="lysS"
                     /locus_tag="ACUQR0_003530"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HHV9110409.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8810..9358)
                     /gene="idi"
                     /locus_tag="ACUQR0_003531"
     CDS             complement(8810..9358)
                     /gene="idi"
                     /locus_tag="ACUQR0_003531"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HHV9110410.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            9413..9484
                     /locus_tag="ACUQR0_003532"
     CDS             9413..9484
                     /locus_tag="ACUQR0_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110411.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(9481..9606)
                     /gene="yqfG"
                     /locus_tag="ACUQR0_003533"
     CDS             complement(9481..9606)
                     /gene="yqfG"
                     /locus_tag="ACUQR0_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HHV9110412.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(9608..>9863)
                     /locus_tag="ACUQR0_003534"
     CDS             complement(9608..>9863)
                     /locus_tag="ACUQR0_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HHV9110413.1"
                     /translation="SLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFF
                     NGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         2413 a         2466 c         2677 g         2307 t
ORIGIN      
        1 cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt gataaaggca
       61 tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat gcctgatgcg
      121 atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt gtattcgcat
      181 cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg acctgtacca
      241 cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga cgccagttct
      301 cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctgaca ctacctgcca
      361 gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc atctcttgtc
      421 cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg gtcgcctgtg
      481 ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca atgtttaatg
      541 ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg gtcagcatat
      601 tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca aaccatagca
      661 aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg ctaaagagat
      721 ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc tcgtcgtctg
      781 gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc tgcggttcac
      841 gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga cgtaaattgc
      901 tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct tctgccatct
      961 ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg ccagtaatgg
     1021 tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca gaagccgtag
     1081 gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct taacggcgta
     1141 ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta cgcgatctgt
     1201 gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga attaatttcg
     1261 cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac gttacaatag
     1321 ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa agcccgaatt
     1381 cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc gtttttcgaa
     1441 catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct gctggaatgt
     1501 gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga tgcagaactg
     1561 gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt ggcaatctga
     1621 tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc tggttgccgc
     1681 tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg tgttactttc
     1741 gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc agggggaaat
     1801 tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca tcgtcaaagc
     1861 accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg gtaaacggca
     1921 acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt tacggcggat
     1981 tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg ctaagccgtc
     2041 agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac cagctctgaa
     2101 tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc acgaacagtt
     2161 gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt ggccagtagc
     2221 cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc gcatcgagga
     2281 accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt ttaccttcaa
     2341 ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt tcaccaaaat
     2401 cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc ggggagtcgt
     2461 ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt ttttctgccg
     2521 ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata acgtaagctt
     2581 ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc acggcacaat
     2641 aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg agctggcacg
     2701 catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa atacgttgaa
     2761 cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg ggttgacgct
     2821 ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg agggcgggta
     2881 taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc agtatcttta
     2941 tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga aattgcccca
     3001 gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg cgccattaat
     3061 tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg ctaccgggct
     3121 gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc agggcgtagt
     3181 gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag aggcggttta
     3241 ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg tgtcaattga
     3301 cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct ggcaccgcat
     3361 taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc atgtgttgcg
     3421 tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg tgcagatgct
     3481 actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta ccgagcgtct
     3541 gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa cgggaagatt
     3601 tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct ttgctcaggc
     3661 tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca gcgatattca
     3721 gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt tgtacatcac
     3781 cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca cggctccggt
     3841 caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag agatgatcgt
     3901 ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta cctgtggtta
     3961 ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca ccgtgcgtta
     4021 tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga aagctatctg
     4081 gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa gcgtcgcacc
     4141 agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc ttggcgttag
     4201 cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc agccgccgaa
     4261 agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat aattcgcgtg
     4321 aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt gcccgctgaa
     4381 ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc gcaagaactg
     4441 gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga aaaggccgtt
     4501 gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg tgatttcgac
     4561 gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct tggttgcagc
     4621 aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag cccggaagtg
     4681 gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa cggtatttcc
     4741 tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt taccgatcac
     4801 catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa cttgcgcgac
     4861 tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct gatgctggcg
     4921 ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc aattcctaac
     4981 ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt gccgctggac
     5041 gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg aaagtgccgt
     5101 ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact agccgccagc
     5161 gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga tgatatgtcc
     5221 gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct ggcaaatgaa
     5281 ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt tgaagccctg
     5341 accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct ggcaatgtat
     5401 caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa agagcgtttt
     5461 caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg ttccggtcgc
     5521 tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact ctaccccggc
     5581 atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga agaggataaa
     5641 ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc cccttcgcta
     5701 ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac catggaagtg
     5761 gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc gctgtttgac
     5821 ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa ggtgatggtc
     5881 gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga taccgctctc
     5941 tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat caacgagttt
     6001 cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta gcgtcatctt
     6061 ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca ttttgactag
     6121 ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg cattcaggac
     6181 ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca agaaagagcg
     6241 tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac ccgaacgcgc
     6301 acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc tcgaccaaat
     6361 gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag ctgacgacga
     6421 agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac tggcgcagct
     6481 tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc tcgatattca
     6541 ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc gtatgtatct
     6601 gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg aaggtgaagt
     6661 ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg gctggctgcg
     6721 tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg gcggtcgtcg
     6781 ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata ttgatatcga
     6841 aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg gcggtcagca
     6901 cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga tcgtgaccca
     6961 gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc agatgaaagc
     7021 gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga tggaagataa
     7081 caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg actcccgcat
     7141 taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg acggcagcct
     7201 ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca tgtctgaaca
     7261 acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa cgcgtcgtga
     7321 gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc gtcgcgatca
     7381 tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac tggaagcgct
     7441 gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg gtaaagcgtc
     7501 tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc gtgacgatct
     7561 gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca tcctcggcgc
     7621 gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta ccgagctgcg
     7681 tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc aggatcagga
     7741 agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc gtaacacctt
     7801 taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc gcggctttat
     7861 ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc gtccgtttat
     7921 cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg aactgtacct
     7981 caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta acttccgtaa
     8041 cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct acatggctta
     8101 cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc tggcacagga
     8161 tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg gtaaaccgtt
     8221 cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg acatggcgga
     8281 tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc acgttgagaa
     8341 gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag aagcacatct
     8401 gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg cgcgtcgtaa
     8461 cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc gtgaaatcgg
     8521 taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc tggatcaggt
     8581 tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt acgttaccgc
     8641 actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc gtatggtaat
     8701 gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga tgcgtccggt
     8761 gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt tatttaagct
     8821 gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc accatccacg
     8881 gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa tcacaccatt
     8941 gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt gcggcaaata
     9001 ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag cgaaagtcag
     9061 gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa cggcggatca
     9121 ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta gtccacacgc
     9181 caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc ttggcattaa
     9241 acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg gcatactttt
     9301 ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc gtttgcataa
     9361 tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg aaatgattaa
     9421 ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg taaaaaccat
     9481 ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa cacagtcaga
     9541 aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc gcattaaaaa
     9601 attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc cacatacgta
     9661 ctgtcctgac ttctaaatct ttatcagaga cggattcctt caccaggtca gcatgatgct
     9721 gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc ataatgccgc
     9781 tatgtagcag cggttgcagt acggccggta acttagaaaa gaaatcgtga gagagcgtcg
     9841 gagtcatgcc aacgccgaga ctg
//
LOCUS       DBKELA010000130         9861 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.130, whole genome shotgun sequence.
ACCESSION   DBKELA010000130 DBKELA010000000
VERSION     DBKELA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.130"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            393..2051
                     /locus_tag="ACUQR0_003535"
     CDS             393..2051
                     /locus_tag="ACUQR0_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HHV9110414.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="ACUQR0_003536"
     CDS             complement(2048..3004)
                     /locus_tag="ACUQR0_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HHV9110415.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="ACUQR0_003537"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="ACUQR0_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HHV9110416.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="ACUQR0_003538"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="ACUQR0_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HHV9110417.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="ACUQR0_003539"
     CDS             complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="ACUQR0_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HHV9110418.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6660..6734
                     /gene="yljB"
                     /locus_tag="ACUQR0_003540"
     CDS             6660..6734
                     /gene="yljB"
                     /locus_tag="ACUQR0_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HHV9110419.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6833..7153
                     /gene="clpS"
                     /locus_tag="ACUQR0_003541"
     CDS             6833..7153
                     /gene="clpS"
                     /locus_tag="ACUQR0_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HHV9110420.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7184..9460
                     /gene="clpA"
                     /locus_tag="ACUQR0_003542"
     CDS             7184..9460
                     /gene="clpA"
                     /locus_tag="ACUQR0_003542"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HHV9110421.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         2422 a         2293 c         2740 g         2406 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc
     6241 gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc
     6301 ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc
     6361 aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata
     6421 atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt
     6481 gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa
     6541 gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa
     6601 atacaattta gagaattagg gagagccgtc aagcatttga caggggccag ggggcaggta
     6661 tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg
     6721 ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt
     6781 gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa
     6841 aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa
     6901 accgccatct atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt
     6961 tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct
     7021 cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac
     7081 caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct
     7141 agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact
     7201 ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac
     7261 cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc
     7321 gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac
     7381 acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg
     7441 tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc
     7501 aaacgttctg gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa
     7561 acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga
     7621 gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga
     7681 ggaacgtatg gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga
     7741 cccactaatt ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg
     7801 taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg
     7861 tcttgcctgg cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta
     7921 ctctctcgat atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg
     7981 ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga
     8041 gatccacacc attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct
     8101 aatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga
     8161 gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat
     8221 tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga
     8281 agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt
     8341 gaaatacatt aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcggg
     8401 cgctcgcgca cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat
     8461 tgagtccgtg gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg
     8521 cgataccctg aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc
     8581 cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa
     8641 accggtcggt tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt
     8701 acagctttcg aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga
     8761 acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg
     8821 cggtttgctg accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat
     8881 cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct
     8941 gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa
     9001 cgccggggta cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac
     9061 cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa
     9121 cattatctgg ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat
     9181 cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc
     9241 gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg
     9301 tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt
     9361 ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt
     9421 ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg
     9481 gtgaagtccg taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa
     9541 aaccctattt ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga
     9601 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
     9661 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
     9721 tttgcgaacg cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac
     9781 ccgcccacca tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
     9841 cgtacgagct cttctttaaa t
//
LOCUS       DBKELA010000131         9641 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.131, whole genome shotgun sequence.
ACCESSION   DBKELA010000131 DBKELA010000000
VERSION     DBKELA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.131"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..165)
                     /locus_tag="ACUQR0_003543"
     CDS             complement(<1..165)
                     /locus_tag="ACUQR0_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HHV9110422.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFAL"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="ACUQR0_003544"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="ACUQR0_003544"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HHV9110423.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="ACUQR0_003545"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="ACUQR0_003545"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HHV9110424.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="ACUQR0_003546"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="ACUQR0_003546"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HHV9110425.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="ACUQR0_003547"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="ACUQR0_003547"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HHV9110426.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="ACUQR0_003548"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="ACUQR0_003548"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HHV9110427.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="ACUQR0_003549"
     CDS             5060..6193
                     /locus_tag="ACUQR0_003549"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HHV9110428.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="ACUQR0_003550"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="ACUQR0_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HHV9110429.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="ACUQR0_003551"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="ACUQR0_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HHV9110430.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="ACUQR0_003552"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="ACUQR0_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HHV9110431.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="ACUQR0_003553"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="ACUQR0_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HHV9110432.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="ACUQR0_003554"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="ACUQR0_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HHV9110433.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="ACUQR0_003555"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="ACUQR0_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HHV9110434.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2472 c         2459 g         2259 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt t
//
LOCUS       DBKELA010000132         9638 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.132, whole genome shotgun sequence.
ACCESSION   DBKELA010000132 DBKELA010000000
VERSION     DBKELA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.132"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(49..369)
                     /gene="psiF"
                     /locus_tag="ACUQR0_003556"
     CDS             complement(49..369)
                     /gene="psiF"
                     /locus_tag="ACUQR0_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HHV9110435.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(488..1903)
                     /gene="phoA"
                     /locus_tag="ACUQR0_003557"
     CDS             complement(488..1903)
                     /gene="phoA"
                     /locus_tag="ACUQR0_003557"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HHV9110436.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2004..2264)
                     /gene="iraP"
                     /locus_tag="ACUQR0_003558"
     CDS             complement(2004..2264)
                     /gene="iraP"
                     /locus_tag="ACUQR0_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HHV9110437.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2446..2649)
                     /locus_tag="ACUQR0_003559"
     CDS             complement(2446..2649)
                     /locus_tag="ACUQR0_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110438.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2727..3821
                     /gene="ddlA"
                     /locus_tag="ACUQR0_003560"
     CDS             2727..3821
                     /gene="ddlA"
                     /locus_tag="ACUQR0_003560"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HHV9110439.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3845..4057)
                     /locus_tag="ACUQR0_003561"
     CDS             complement(3845..4057)
                     /locus_tag="ACUQR0_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HHV9110440.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4317..4625
                     /locus_tag="ACUQR0_003562"
     CDS             4317..4625
                     /locus_tag="ACUQR0_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HHV9110441.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4684..5778)
                     /gene="yaiW"
                     /locus_tag="ACUQR0_003563"
     CDS             complement(4684..5778)
                     /gene="yaiW"
                     /locus_tag="ACUQR0_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HHV9110442.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5791..7011)
                     /gene="sbmA"
                     /locus_tag="ACUQR0_003564"
     CDS             complement(5791..7011)
                     /gene="sbmA"
                     /locus_tag="ACUQR0_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HHV9110443.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7363..8520
                     /gene="ampH"
                     /locus_tag="ACUQR0_003565"
     CDS             7363..8520
                     /gene="ampH"
                     /locus_tag="ACUQR0_003565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HHV9110444.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8521..9024)
                     /locus_tag="ACUQR0_003566"
     CDS             complement(8521..9024)
                     /locus_tag="ACUQR0_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9110445.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9112..>9638)
                     /locus_tag="ACUQR0_003567"
     CDS             complement(9112..>9638)
                     /locus_tag="ACUQR0_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHV9110446.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2396 a         2460 c         2292 g         2490 t
ORIGIN      
        1 tcacagataa aacactctcc aggaaacccg gggcggttca atcaggtatc aggcggcttt
       61 cttgaggcag gcactcataa acttattacg atcatcacct ttcagagctt gttgcgttgc
      121 ttgattatta cattcacgca tcttttgctg ctgtggcgtc aaactttttt caccaggcgc
      181 agacttgctg ttcttcaggc aatcactcat gtaggtctta cgagcatccc ctttcaacgc
      241 ctgcgccgtc gcctgctgat tacaggaggt catacgctgt tgttgtgggg ttaaggttct
      301 ctcggcagcg ccgacggtgg ttaaaaaaac cagaccaaaa agcaaggtaa ccagtaatgt
      361 tattttcata gcaccatccc tcttcatgtt ttaaccatga gcgtttgcgc ccgtgatctg
      421 ccattaagtc tggttgctaa cagcaaaaaa accacccggc agcgaaaatt cactgccggg
      481 cgcagtttta tttcagcccc agagcggctt tcatggtgta gaagagatcg gtctggtcgg
      541 tcagtccaac aacattggcg gcatgcgggc catacgccgc aatacgcaac tgactgccgg
      601 tatgttcttg tgaatcctct tcggaattcc cgtaactcat caccatcact gcgccatctt
      661 tagtatttag cgcctgagtg aggcccggcg ctttggtatc cggcgcaaca acctggctgg
      721 cgtgggcgtg atcagcggtg actatgacca gcgtgttacc ctccttttta gcgaattcca
      781 gcgcccgttg tacggcttca tcgagatcga ccgtctcgcc aatttgccca caagggttcg
      841 cagcgtgatc ctgtttatcg attgacgcac cttcaacttg caggaaaaat cctttctcat
      901 ttttactcaa caattcaatg gctttgtcgg tcatctgtgc cagggttggt acactgtcat
      961 tacgttgcgg attaggcgta taggtgactg cgggcttatc gatattgccg tggtacgttg
     1021 ctttcggccc tagccagcgc actggcatat tgccgtcagc aaacaggcca agcaggggtt
     1081 tttgctgatt cgcttccgtc accgaattca gtgaggcagc atcgttcacc aactgataac
     1141 cacgcgcctg tgcctgttca cgcagcgttt ttccctgcca ttcaccagcg gttgccgttt
     1201 cagcaaaggt ttttgcgccg ccgccaagcg taacgtcggc acgagcgtta agcagctgtt
     1261 cggtaatcga tccttttccg cctttttcca gagcgttacc cggacatttt tcactggtcg
     1321 cgctcggacc gtagcatttg cgcgaagtca catgtgccac cagcgcagcg ggcgtggcat
     1381 cctgcaactc tgcggtagaa acgttaccgg tcgccagacc tgcggctttt gccatttcca
     1441 gaatcgttgg gtgatctttt tcgtgaatat cgacgcccag cgcgccgtta taggttttga
     1501 caccggttga ccaggcggtt gctgatgcag ccgagtcggt gacgtagtcc ggtttgccag
     1561 tttttttatt cagcgcatag tgagtgtatt gcccggtaag cggtaaggca tcgatacctt
     1621 taaaaaagcc gcccgcacct tcggcataat tacgtgcggc agtaatttcc gaatccccca
     1681 tcccatcgcc aatcagcaaa ataatatttt ttgcaggttt atcgctaaga gaatcacgca
     1741 gagcggcggt ctgatcaccc gttaagcggc gagcaccgcc gggtgcagta atatcgccct
     1801 gagcagcccg gttttccaga acaggcattt ctggtgtccg ggcttttgtc acaggggtaa
     1861 acagtaacgg taagagtgcc agtgcaatag tgctttgttt cactttattt tctccatgta
     1921 caaatacatt aaaaaataaa aacaaagcga ctataagtct cggccgtgac aactttatga
     1981 cagctgttga aaagattaac tttttactga caaggatgct tcaataactt ctttacgtaa
     2041 tcgcgcagca gctctgtatc gtcgtcagga atgctggcat cgggctttac ctcgtacagc
     2101 gccccctcta cctgatcaat caaccgctgt tggtcatttt gcgccatatt gcgaagcatt
     2161 gcagtgacga taatctccaa agcttctacc tgcgcacaca gttctttcga ctcttcttct
     2221 ttttgggcaa gcttaaataa caactcagca atgagatttt tcatgtctgt atttccttat
     2281 ccaaagtatg gagaagttaa cattatgttt cacaacttca tagtgtctgg aagtattatt
     2341 tttatgacaa aggaaatact ttgcgcaaat attttttgtt tgattaccag cgaaacgttt
     2401 ctcttattta aataattacc cacacaaaat ataggctttt aattatcaac ctttattata
     2461 aataaaagct atgaaatata ttctcttcgt taacttataa ttaagagaaa aaaactcacc
     2521 gcattgccat ttaagccagg aataaattag cttcccgttc atcatttttg tgatcttgcg
     2581 cacatctttg agataacgca ctttcttcac cagtacgttg cacgctttgt cagcagaaga
     2641 ttgcaacaat ctttcagaag gccgcgaatc ccccgttttg tcgtggtaat gtatgcactt
     2701 tgtgttagac gggataggtt tttaagatgg aaaaactgcg ggtaggaatc gtttttggtg
     2761 gtaaatcagc ggaacatgaa gtgtctctgc aatcggcaaa aaacattgtc gatgccattg
     2821 ataaaagtcg cttcgacgtt gtgctgctgg gcattgataa acaagggcaa tggcacgtca
     2881 gcgatgccag caattatctg ctaaatgcag acgatcctgc ccatattgcg ttgcgccctt
     2941 cggcgaccag ccttgcgcag gtgccaggta aacatgagca tcagcttatc gacgcgcaaa
     3001 acggtcagcc gttgccgacg gtggatgtca ttttcccgat tgtccacggt acgctgggcg
     3061 aagatggttc cttgcaggga atgctgcggg tcgccaattt accgtttgta ggttctgatg
     3121 ttctggcttc agcagcctgt atggataaag atgtcaccaa acgtctgcta cgcgatgccg
     3181 ggctgaacat tgcgccattt attaccctga cgcgcgctaa tcgtcacaac atcagttttg
     3241 ccgaagtgga gtctaaactg gggttaccac tgtttgtaaa accggctaat cagggctctt
     3301 ctgttggtgt cagcaaagta accagtgaag aacagtacgc aattgccgtc gatctggcgt
     3361 tcgagttcga tcataaagtg atcgttgagc aagggatcaa aggtcgtgag atcgaatgcg
     3421 cagttctggg caacgacaat ccgcaagcca gcacctgtgg cgagatcgta ctcaccagcg
     3481 atttctatgc ctacgacacc aagtacattg acgaagatgg cgcgaaagtg gtagttccgg
     3541 cagccattgc accagagatc aacgataaga tccgggcgat tgccgttcag gcttatcaga
     3601 cgttgggatg cgcaggcatg gcgcgtgtag acgtgttttt aaccccagag aacgaagtgg
     3661 tgatcaacga gatcaacaca ctgcctggct tcaccaacat cagtatgtat ccgaagctgt
     3721 ggcaagccag cggtctgggt tacaccgatc tgatcacacg tttgattgag ctggcgctgg
     3781 agcgtcacgc tgcggataac gcattgaaaa ccacaatgta atattttaac gcccgataat
     3841 attcttattg ggcgttttca gtttcttcat cgcgacgacg aataatcaac cccgccagcc
     3901 agaaactaat cacccacgtc accagaccga cggcataggt ctgccaacct tttgcttcaa
     3961 atcccagtac ccccaccacg ccattaagaa tgaatataag gccaatggcg aacgcatagt
     4021 agtgccagtc acggcggatt tttacaggca gattcatggc agctccgtta acgaaaaaca
     4081 acatccttgc atattctgta acaaccgtct gtggtgtaga caacattttc gtaattatta
     4141 ataaatttaa cttaaggttg ccattttgtg actcacaacg aagattcacg cttcgggaat
     4201 attcccaaca tcaatttacc atgattctga tattgacaat ctaaatgacc tgtcagactt
     4261 gccataagcc gctggtatca caccagaaga gaagcagatg acttccggag gtttttatgg
     4321 ctgatttcac cctgtcaaaa tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc
     4381 ctggcaacat tgcctatgcg ttgtttgtgc tgttttgctt ttgggctggg gcgcaattgc
     4441 tgaacctgtt agtgcatgcg cccggcgtct atgagcgttt aatgcaggtc caggaaacag
     4501 gtcgcccacg ggtggaaatt ggtttaggtg tcggcaccat tttcgggctg atcccgtttt
     4561 tagcaggctg cctcattttt gcagtggtgg cgctatggct gcactggcga catcgccgcc
     4621 agtaaaattt acgttgccag atgcggcctg aaacgcctta tctggcctga cattcctgtt
     4681 tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt
     4741 agtcaggttg cgggtgattt tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg
     4801 tagtgattta cccgttttcg cttcggcaag ttgataaact ttcttgtaca gcgccgtctc
     4861 ctcaaaagag aagctgtcac ctttctctaa ctgacggcga atttcgctgt cgctcatccc
     4921 cagttttccc gccagtttgc gcgttgccag ttcggttttc ctgggttctt tactgtcgta
     4981 acgaattaaa tcaccgtcca gtgccagctt cacgccgctg gccttactga ctgcgttctg
     5041 aaacgcggca ttacggctgg cgtaccagcc agcgttaaaa tcagcaaagc ggtatatcgg
     5101 tgcgctatag ctggcgggat aattcagtaa atggtaagta ccaaaccaca acccgccgcg
     5161 gcgactgaag acttcctgac gaactgtacc gtccattttc cacggatacc ctttggtatg
     5221 ctgttcggca aaagcaatgc tgacctgcat cggcccaccg gtgcgcaccg gattaagcga
     5281 accaaacagc gtctgcccca ttggtaccat gctgatcaga tcgtcaaaaa tcgcgctcaa
     5341 ttgcttttct gtacgcaccg aatctaaccg ttcgctataa cttttgccgt ttggcgattt
     5401 gatttttagc gcggtatgaa ccagaaaagc aggaatgtgc atccgttcag cacgacggtc
     5461 aatctcttgc caggcgatct tgcttaagcc cggaacggcc ggatctgcct gataatttga
     5521 ctcctgctgc gccaccgcca gcaccgagta gacgttttcc agcgtcggtg ccagcccctg
     5581 actttcaaag gtggtggcga gatctttcgc ccaggcatcg cggtctttca cgctcgcagg
     5641 catcttctgc cgcacgacgc tcgctacatc tatcgctttt tcgccctttt tcaacggttg
     5701 cggagcctga ctactacacc ccgctaaaac cagaaccgcc aacaatgaca gcgaagatag
     5761 atgattgacg cgcgacatta cttctccttt ttagctcaag gtatgggtta cttcctgaat
     5821 tttgtcacca tccagctcat gttcaaagct gcgcagacgt ttgtagatag acatcaactc
     5881 aaccagtgtg gtccatgagt taatcaggta ctggaaagca ccgcgaacct gaccaaaaac
     5941 gttggtaatc tgcgtcatca ggccgagcgt aatcgtaccg gcaacaattg acggaaacag
     6001 caagaacaaa ccgaaaacgt tatcgacctg caaatagaga atgcgggcga tgttgaaata
     6061 catatagtga aaatagaggc gaaaatagtt tttccgtacg gcgctaaaca gctcgcgtac
     6121 cgtaggcggc gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt tacggtaggc
     6181 ggcctctact cgctggtttt taaactccag ccccggcagt ttgatcccta ccactgccag
     6241 caatccggtc cccatcagcg accagacgat agcggcaatc accagaccat acggaatgtg
     6301 cccgataatc ggcagctccg gcacatgcgc ggagagcgtt accagcaccg gcaggaaggc
     6361 gatcaacgtc attatggcgt tgataaaact gacgcccata ttctccagcg ttgaagcaaa
     6421 acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg atatgacgca gttgttgcca
     6481 gttcgccatg taatattcgt tcatcgccgt acgccagcgg aacacgtagt gactgacaaa
     6541 gaagttgttc agtacactga tcaccactgc gatcagcgca atccccagaa agacgcccac
     6601 ttcgcggtaa aattgttcga tagtgacttt atgcggcgaa cttagcgcgg tttgaatcag
     6661 atcatagaac ggcgcatacc aggcgttgac ggcgaccccg acttccacca aaaaccaagt
     6721 gacgaagata atcagcgcag taccgagtat tgaccagtat tgccaacggt gcgggctgta
     6781 gataaaccag aaaaatgcaa aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag
     6841 gaaatccaac gaccagaaac gcgcggcgct aatcgggatc tgcccggaag cgccggtgat
     6901 atgtgccacc cagtcacccc caccggcttg ccagaagata acggcaatca atgcccaaac
     6961 aaaggccgag agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca tatgcactcc
     7021 tgctaacttc ttatcgtttt aacaactgct gtgtattaac cgcatgaccg ctacaaagcg
     7081 tcagccaaat gaaaccgccc attgtgaccc atagcaaggc gcttagttcg ccagcctgac
     7141 cataagaatt gtttcgacgg atttcacaaa ccaaagattg tttacataag cggattaaca
     7201 cgcgcaggat cacaaatgca ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt
     7261 tacgttttta acactattat tgcactaatt cgccctttgc aatccatcaa tgagtagtat
     7321 aaatacgctc agttaccttc attcaatcta tggacaccac cgttgaaacg tagtctgctt
     7381 ttttctgccg tgctgtgtgc ggcgtcattg acctccgtcc atgcggcgca gccgatcact
     7441 gaaccggagt ttgcctctga tattgtcgat cgttatgccg atcatatttt ttacggcagc
     7501 ggtgccacgg ggatggcgct ggttgttatc gacggtaacc agcgcgtctt tcgtagttat
     7561 ggcgaaacgc gacctggtaa taacgttcgc ccgcagctgg attcggtcgt gcgtattgct
     7621 tccctcacca aactaatgac cagtgaaatg ctggtgaaat tgctcgacca ggggaccgta
     7681 aagttaaacg atccgctaag taaatatgct ccgccaggcg cacgcgtgcc gacctacaat
     7741 gggacgccca ttaccctagt gaatctggca acccatacca gcgccctgcc ccgtgaacag
     7801 cccggtggcg cggcacatcg tccggtgttt gtctggccaa cgcgcgagca acgctggaaa
     7861 tacctttcta cggcgaagct gaaagccgcg ccaggcagtc aggcggcgta ctctaacctt
     7921 gcgttcgacc tgctggcaga tgcactggcg aatgcgtccg gcaagcctta tacccagttg
     7981 tttgaagagc agattaccca tccattgggg atgaaagaca ccacctacac cccctcaccg
     8041 gatcagtgcc gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg
     8101 gcggcaattg gcagcggcgg cgtctattcc acgcctggcg atatgatgcg ctggatgcag
     8161 cagtatttgt catctgattt ttatcagcgc agtaaccagg cagatcgcat gcagacgttg
     8221 atttatcagc gcgcgcagtt tacgaaagtg attggcatgg atgtccccgg caaagcggat
     8281 gcgcttggtt taggctgggt atatatggcc ccgaaagagg gtcgtccgag gattattcag
     8341 aagacaggcg gtggtggcgg tttcattacc tatatggcga tgatcccgca gaagaatatt
     8401 ggcgcgtttg tggtggtcac tcgttcgccg ttgacgcgct ttaaaaatat gagcgatggc
     8461 atcaatgatc tggtgaccga gctaagcggg aataaaccgt tggttatccc cgcatcctga
     8521 ttaatactct gaaggcaaac ggttgatagc gaccagtttg cctttattca tttcgatata
     8581 gccgccttta cgcaaagcag cgagaacttc tgcgacgaca gaacgcgata tgcgtgtacg
     8641 ttgatggata taattcatca caccaatacg tgatcgcaat tcttcattcc agtcaatcat
     8701 tgataataat gttgcgcggg tttgtttggc tggtagatga tatcccgtac aatttccttc
     8761 tgatattaat ttgtatggta tatcgttttt cattaaacca tcagccagcc ccataatata
     8821 aggagcctgg gtaataccga taagtacgtt ttcttctcta cgcagagaga taactccttc
     8881 taaaataacg aaagtatctt cgttattgac atcactggaa aatataactt gcttttcatt
     8941 attaaactcg aagcgcgtac cgtatctgga caaacattta tcgagcttac caaattcctg
     9001 aagaggttta actacagata acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt
     9061 gacgggcatt atttagatta aattaccagt atttcttcgg agtgaagaat attaccaagt
     9121 atatttaaca cccacgttcg cggaccagtc ttgatctacg tcaccaccac cgaggtagtt
     9181 agcatcggta taggcgctga agttcttggt gaagctgaac tgagtaccca gaccaacacg
     9241 taccgcagac ccttcagtac cgttatcgat ggaatcaccg ttcacatcgt tatcgttgtt
     9301 agagtcgtcg tagacgtaag ccagtttgaa gtacggagtc agagcctgat cttcgctgta
     9361 ggtgaaggta taacctgcat ctacacccag ttcataacgc atgctgtcgt aaggctgacc
     9421 gtcaactttc atgtcgttgc tcagctggta gtcatcacca gactggaaca gaccagaaat
     9481 gctgccgtaa ggagtcacgt aaccagcatc acccagtttg aagtcgtaac cggttttcaa
     9541 accgaagccc caagcgtcgg agttggtgct accgtcaacg taagtaccgt tgctcatggt
     9601 tgcagacagg tcgttgttga agtgagagta gctcaagc
//
LOCUS       DBKELA010000133         9626 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.133, whole genome shotgun sequence.
ACCESSION   DBKELA010000133 DBKELA010000000
VERSION     DBKELA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.133"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="ACUQR0_003568"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="ACUQR0_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HHV9110447.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="ACUQR0_003569"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="ACUQR0_003569"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HHV9110448.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="ACUQR0_003570"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="ACUQR0_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4043)
                     /gene="rhaR"
                     /locus_tag="ACUQR0_003571"
     CDS             complement(3195..4043)
                     /gene="rhaR"
                     /locus_tag="ACUQR0_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HHV9110449.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="ACUQR0_003572"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="ACUQR0_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HHV9110450.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="ACUQR0_003573"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="ACUQR0_003573"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HHV9110451.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="ACUQR0_003574"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="ACUQR0_003574"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HHV9110452.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8271..9032
                     /gene="rhaD"
                     /locus_tag="ACUQR0_003575"
     CDS             8271..9032
                     /gene="rhaD"
                     /locus_tag="ACUQR0_003575"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HHV9110453.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="ACUQR0_003576"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="ACUQR0_003576"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HHV9110454.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2270 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaatt
//
LOCUS       DBKELA010000134         9483 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.134, whole genome shotgun sequence.
ACCESSION   DBKELA010000134 DBKELA010000000
VERSION     DBKELA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.134"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..180)
                     /locus_tag="ACUQR0_003577"
     CDS             complement(<1..180)
                     /locus_tag="ACUQR0_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HHV9110455.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLG"
     gene            700..1395
                     /gene="minC"
                     /locus_tag="ACUQR0_003578"
     CDS             700..1395
                     /gene="minC"
                     /locus_tag="ACUQR0_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HHV9110456.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1419..2231
                     /gene="minD"
                     /locus_tag="ACUQR0_003579"
     CDS             1419..2231
                     /gene="minD"
                     /locus_tag="ACUQR0_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HHV9110457.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2235..2501
                     /gene="minE"
                     /locus_tag="ACUQR0_003580"
     CDS             2235..2501
                     /gene="minE"
                     /locus_tag="ACUQR0_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HHV9110458.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2873..3217)
                     /locus_tag="ACUQR0_003581"
                     /pseudo
     CDS             complement(2873..3217)
                     /locus_tag="ACUQR0_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3252..3371)
                     /locus_tag="ACUQR0_003582"
     CDS             complement(3252..3371)
                     /locus_tag="ACUQR0_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HHV9110459.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3332..3517)
                     /gene="ymgJ"
                     /locus_tag="ACUQR0_003583"
     CDS             complement(3332..3517)
                     /gene="ymgJ"
                     /locus_tag="ACUQR0_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HHV9110460.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3618..3791
                     /gene="ymgI"
                     /locus_tag="ACUQR0_003584"
     CDS             3618..3791
                     /gene="ymgI"
                     /locus_tag="ACUQR0_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HHV9110461.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3793..4137
                     /locus_tag="ACUQR0_003585"
     CDS             3793..4137
                     /locus_tag="ACUQR0_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HHV9110462.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4141..4476
                     /locus_tag="ACUQR0_003586"
     CDS             4141..4476
                     /locus_tag="ACUQR0_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HHV9110463.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4533..6854)
                     /locus_tag="ACUQR0_003587"
     CDS             complement(4533..6854)
                     /locus_tag="ACUQR0_003587"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HHV9110464.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6971..7180)
                     /locus_tag="ACUQR0_003588"
     CDS             complement(6971..7180)
                     /locus_tag="ACUQR0_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110465.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7580..7799)
                     /gene="ymgF"
                     /locus_tag="ACUQR0_003589"
                     /pseudo
     CDS             complement(7580..7799)
                     /gene="ymgF"
                     /locus_tag="ACUQR0_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(7931..9454)
                     /locus_tag="ACUQR0_003590"
     CDS             complement(7931..9454)
                     /locus_tag="ACUQR0_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HHV9110466.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2200 c         2078 g         2546 t
ORIGIN      
        1 gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac aaatatacgg
       61 gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc cagacgccat
      121 tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca tcatattcat
      181 agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc aatccgtaat
      241 caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac gtcttctgcg
      301 gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct gttctttaat
      361 gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata cacttgcctc
      421 aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg gaaaaaggaa
      481 gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag ggtatttttt
      541 aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata aatgggaggg
      601 tgacttgcct caatataatc cagactataa catgccttat agtcttcgga acatcatcgc
      661 gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac tccaatcgag
      721 cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga acctaaggtt
      781 atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa acatgccccc
      841 gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat gcataaggcg
      901 gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc gcaacttaaa
      961 gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa agcgccacgt
     1021 ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa aacgcgttta
     1081 atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga tctgattgtt
     1141 acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca tgtctatggc
     1201 atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca aatattttgt
     1261 acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag tgatcaaatc
     1321 ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc tttgaccgtt
     1381 caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt attgttgtta
     1441 cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc actggtttgg
     1501 cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt aatctcgacc
     1561 tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt cagggcgatg
     1621 caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat attcttccgg
     1681 catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa gttcttgatg
     1741 atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg attgaaaccg
     1801 gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac ccggaagtct
     1861 cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca cgccgcgcag
     1921 aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac ccaggccgcg
     1981 taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc atcaaactcg
     2041 tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt gaaccggtca
     2101 ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa cgtctgttgg
     2161 gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc aaacgcttgt
     2221 tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag aacacagcca
     2281 acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc gatgcagaac
     2341 cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa tacgtacaaa
     2401 ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt tctattcttg
     2461 agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt aaaaacgcat
     2521 ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat ccacgttgtg
     2581 gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc cccctttaca
     2641 ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt aaataaccat
     2701 gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt cattgttctc
     2761 ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga attttctctt
     2821 tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat ggtcagaacg
     2881 accagttcac accagccacc gcgttccacg gggactccac accggcacca tggctatacc
     2941 ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct gcctggtata
     3001 ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact ttcacccgat
     3061 tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt tccataccgt
     3121 tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata ctgcgggtat
     3181 cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt gacctaagct
     3241 ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag agacagcgca
     3301 ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata tcccgcataa
     3361 gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa atcactcttt
     3421 tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa aataaaatga
     3481 ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac aataatattg
     3541 ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca cttcttgaat
     3601 aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt cacgtaaaaa
     3661 acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat agcgagcgtt
     3721 ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact tacttaatac
     3781 cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc attgttctgc
     3841 tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct aggcgcaggc
     3901 gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc tgttggcgct
     3961 ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa aggtgcaaaa
     4021 gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct cgaaggggcg
     4081 attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat gaaataagga
     4141 atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc aatgacctgg
     4201 gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac ttgcgcagaa
     4261 gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc ggtaatgggt
     4321 aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact gagcgccaaa
     4381 gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt gatcacggca
     4441 attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac cgcgtcgatg
     4501 aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag gcccaccatc
     4561 actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc ccagacgctg
     4621 gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt actcacccca
     4681 tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt atggatccag
     4741 ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta
     4801 cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc attctcaata
     4861 cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat agcctgtgcc
     4921 tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt attcagggca
     4981 aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga ttcataggct
     5041 aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga gtcaatgtat
     5101 gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc gctatagcct
     5161 ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt gtagtcgcgg
     5221 gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc accggttaac
     5281 agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg tccgttgctg
     5341 tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga acgctcctgt
     5401 tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg cataacaaaa
     5461 agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg caggacgggg
     5521 gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc gctcgttgcc
     5581 tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg ggttaatgaa
     5641 tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt tgattccccc
     5701 tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc tgcattggta
     5761 actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc cgtagctgaa
     5821 ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac gtaattttca
     5881 gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat atagaccgta
     5941 ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca ggtactatta
     6001 tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc tgaacccgtt
     6061 atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat aaaaacatct
     6121 gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt tttatggtag
     6181 aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt tgcttcctga
     6241 ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc agatgtattt
     6301 ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg acccgttgta
     6361 taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc atgagcattg
     6421 tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt ataatcaagc
     6481 gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc tgttccgtgg
     6541 gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc attacttcct
     6601 gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct tccgggagat
     6661 gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt atcatctccg
     6721 tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt gtcagtattc
     6781 aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt gacaatcgtg
     6841 tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct agctgttaat
     6901 gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac aatgacgcca
     6961 ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt ccattacccg
     7021 ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt gccacagcat
     7081 gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc gccagagcaa
     7141 taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta tccattcatt
     7201 ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa acggagggta
     7261 aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata tataaatgat
     7321 aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac tttggggtga
     7381 ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc catggtaatt
     7441 atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta tttgcgcaat
     7501 aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag ccgcatactg
     7561 acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt tctcttaaat
     7621 cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc
     7681 acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt gatcagcata
     7741 aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact attattcatg
     7801 ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa
     7861 tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac
     7921 tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata attttgacca
     7981 ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt
     8041 ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa
     8101 gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttta
     8161 cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca tgaagataag
     8221 aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca
     8281 gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt
     8341 ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga
     8401 caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg
     8461 gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa
     8521 gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc
     8581 caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac
     8641 catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga
     8701 tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca gcggcgcagg
     8761 cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa
     8821 agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg
     8881 tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata atgtcatccg
     8941 ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg cgaatatgtt
     9001 gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag agcaggatag
     9061 gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc gggacaacag
     9121 gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact attccctgtt
     9181 tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca agctgatatt
     9241 gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc gcctgtgacg
     9301 cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc aggtactctg
     9361 cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata aacaagcata
     9421 tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca aagggtttaa
     9481 tac
//
LOCUS       DBKELA010000135         9163 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.135, whole genome shotgun sequence.
ACCESSION   DBKELA010000135 DBKELA010000000
VERSION     DBKELA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.135"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..1335)
                     /gene="mnmE"
                     /locus_tag="ACUQR0_003591"
     CDS             complement(<1..1335)
                     /gene="mnmE"
                     /locus_tag="ACUQR0_003591"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HHV9110467.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGR"
     gene            complement(1441..3087)
                     /gene="yidC"
                     /locus_tag="ACUQR0_003592"
     CDS             complement(1441..3087)
                     /gene="yidC"
                     /locus_tag="ACUQR0_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HHV9110468.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3090..3347)
                     /gene="yidD"
                     /locus_tag="ACUQR0_003593"
     CDS             complement(3090..3347)
                     /gene="yidD"
                     /locus_tag="ACUQR0_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HHV9110469.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3311..3670)
                     /gene="rnpA"
                     /locus_tag="ACUQR0_003594"
     CDS             complement(3311..3670)
                     /gene="rnpA"
                     /locus_tag="ACUQR0_003594"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HHV9110470.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3687..3827)
                     /gene="rpmH"
                     /locus_tag="ACUQR0_003595"
     CDS             complement(3687..3827)
                     /gene="rpmH"
                     /locus_tag="ACUQR0_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HHV9110471.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4057..4137)
                     /locus_tag="ACUQR0_003596"
     CDS             complement(4057..4137)
                     /locus_tag="ACUQR0_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110472.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4434..5837
                     /gene="dnaA"
                     /locus_tag="ACUQR0_003597"
     CDS             4434..5837
                     /gene="dnaA"
                     /locus_tag="ACUQR0_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HHV9110473.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5842..6942
                     /gene="dnaN"
                     /locus_tag="ACUQR0_003598"
     CDS             5842..6942
                     /gene="dnaN"
                     /locus_tag="ACUQR0_003598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HHV9110474.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            6942..8015
                     /gene="recF"
                     /locus_tag="ACUQR0_003599"
     CDS             6942..8015
                     /gene="recF"
                     /locus_tag="ACUQR0_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HHV9110475.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8044..>9163
                     /locus_tag="ACUQR0_003600"
     CDS             8044..>9163
                     /locus_tag="ACUQR0_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9110476.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVG"
BASE COUNT         2150 a         2400 c         2521 g         2092 t
ORIGIN      
        1 ccgccccagc aggtcgtctg aagtaaattc cccggtgatt tcgcttaagt tctgctgtgc
       61 caggcgcaac tcttccgcca gcagttcacc tgcccaggct cccaacagtt gcgctttacc
      121 ttgctgcaaa tgctccgctg cctgttccag cgcctgtaag tggcgacggc gcgccaggaa
      181 gccgccttcc atattggtgt caaagcccat gctctgtttg agatggttac gcagcacgtc
      241 cacgccttca ccagtccttg ccgagagacg aattaacgcg tgaccgttca cttcactcat
      301 tcccagcgtt tcgccggtga tatcggcttt attgcgtacc acggtgatcg gcagtttcgc
      361 gggcagacgg gcgataaatt ccggccagat ctctgccgga tcaacggcgt ctgtcgtggt
      421 gccatcgacc ataaacagca cgcggtcggc ctgttcaatt tcctgccacg cgcgctcgat
      481 accaatacgt tccacttcgt cactggcctc acgtagcccg gcagtgtcga tgatatgcag
      541 cggcattccg tcaatgtgga tatgctcacg cagcacgtca cgcgtggttc cggcgatatc
      601 ggttacgatt gccgcttcac ggcccgccag cgcgtttaac aggctcgatt taccggcatt
      661 aggacgtccg gcaatcacta ctttcatccc ttcgcgcaac aaactaccct gacgtgcttc
      721 agcacgcact gcatcgagat cggccatcac gtcattaagc tgggcttcaa ttttgccgtc
      781 ggagaggaaa tcgatctctt catcagggaa atcaattgcc gcttcgacgt aaatgcgcaa
      841 gtgggtgagg gcttctacca gatgattaac ccgtgcggag aatgcgcctt gcagcgagtt
      901 aagtgccgaa cgggccgcct gttccgaact ggcgtcgata agatcggcaa tcgcctcggc
      961 ctgggctaag tcaagtttat cgttaagaaa cgcgcgttcg gaaaactcac cagggcgagc
     1021 aatccgcagg ccgggaatgg tcagaatgcg ttttaacagc aggtcgagga tcaccggacc
     1081 gccatgacct tgcagttcca gcacatcttc gccggtgaac gagttcgggc cagggaacca
     1141 tagcgcaatc ccctgatcga gcacgctgcc gtcggcgtct ttaaacggaa gataatcggc
     1201 gtagcgcggc ttaggcagtt tacccagcac ggtttcggca acttcacggg ctttgaagcc
     1261 ggagatgcgc aggatgccaa cgccgccacg tcccggaggc gtggcctggg ctacgatagt
     1321 gtcattatcg ctcatgatgt tcctgttgct ttgtgtggcg gatgcgcggt gcttatccgc
     1381 cctacgaaaa gaaaaaaggc ggtcaactga ccgcccttat tttagcgaaa actcaccgaa
     1441 tcaggatttt ttcttctcgc ggctatgcag gccacgtttt tccagaccac ggtaaatcag
     1501 ctgctgctga ataatggtta ccaggttgct gacgatatag tacagtacca gacctgacgg
     1561 gaaccacagg aagaacacgg tgaagatgac cggcataaag gtcatgatct tctgctgcat
     1621 cgggtcggtc acagtggtcg gcgacatctt ctgaatgaag aacatcgtta cgcccatcag
     1681 gatcggcagg atgtagtacg ggtcctgtgc cgacaggtcg tggatccaca gtgcaaacgg
     1741 tgcctgacgc agttcaacgg aacccatcag catgtagtac aacgccagga agattggcat
     1801 ctggatcagc agcgggaagc agccgcccag cgggttaacc ttctcagctt tgtacagcgc
     1861 catcatttcc tgactgatac gctgtttgtc atcgcccaga cgctcacgca ttgcctgaat
     1921 cttcggctgc agcatacgca tcttcgccat ggaggtgtac tgcgctttgg tcagcgggta
     1981 catgatgcca cgaacgataa aggtgatgat gataatggag aagccccagt tacccacaaa
     2041 gctatggatc catttcagca gtttgaacag cggctgagag atgaaccaca accaaccgta
     2101 atcaacggtc agatccaggt gcggagcaac agctgccatt ttgtcctgga tttccgggcc
     2161 aacccacagg gtgctgttca tcgcgccagt ctgaccaggc tgaaccagta ccggctgaga
     2221 tttatagccg atagcgacga tgccgttacc cagattagcg gtatagaagt tgttggtacc
     2281 gtcgttatgc gggatccacg ccgtcgcgaa atactgttgc agcatcgcca cccaaccacc
     2341 tttcgaagag atgttcaggt tttcgttatc ggcaatggta tcgaacttgt atttctcata
     2401 cttctcgtca ggcgtggagt acgccgcgcc acggaaggtg tgcagtgcga agttgctgct
     2461 tccggtatcg agatgcggtg gcagagtgat ggattgcttc aactgaccaa acgaggagat
     2521 ttccagcggt ttctcgccag cgttctgcac gttgtagttg acgttgacag cgtaatcacc
     2581 acgtttcagg acaaacgttt tggtaaacgt gttgcctgcc gcgtcggtat acgtcatcgg
     2641 cacctgcagt tcgttttgac cttcagccag cacataagcg tctttttcaa cgttatacag
     2701 cggacgcggg ccgttagccg ggttatccgg gccatcacga ccggtcagac cgctctgtgc
     2761 ctgataaata aactgcggtg aagtttccaa cagctggaac ggctgggtag agttcagctc
     2821 tttcgggtaa gcaggcagca gagcttgctc aacatcacca ccacgggtgt tgatggtcag
     2881 atcaagcacg tcggtcttaa ccgagatcag tttcccctgg ccactggccg gtacgccctg
     2941 gtcggcggcg ctacccgctg cggtggtcgt tgtctgcgtg gtctgttggg cctgaggttg
     3001 cgggttttta tcctgctccc aggcttgcca gatcatgaaa gacacgaaca gcaaagcgat
     3061 gactaaaaga ttgcgttgcg aatccatcgt tagtgttctc tggtatcaaa tggtccgggc
     3121 gggacgggat cgtcaccacc agggtgtaaa gggtggcatt ttaatacgcg tttcaccgtc
     3181 aaccaactgc cttttatcac tccaaacctg cgcaatgcct caattccgta gcttgaacag
     3241 gttggagtga aacgacaatg cggcccgagt agcggactaa tcaggcgttg atagacccga
     3301 atgagggcta tcaggacccg cgagccaggc gacagtggcg gcgccataat ttttccaacg
     3361 cttccgagag agcacggtta tcgaggtcgg caaccccttt tttcgccacc accacgaaat
     3421 ccatagccgg gagttcatgt tggcgcagac ggaagctttc acgcgtcagg cgtttaatcc
     3481 gattgcgttc atgggcgcgt cgaacgtttt tcttggcgac tgtaagaccg atacggggat
     3541 gccccagcga attcaggcgg ccgagaatgg taatttgcgg cgtgccagcc cgttgtggct
     3601 gctggaagac gaatgtgaat tgactgggag ttaacaagcg taactccctg ggaaatgcga
     3661 gcttaaccac tcaggggtta gctttattac ttagaaacgg tcagacgagc gcggccttta
     3721 gcacgacgac gtgccagaac ctgacgacca tttttagtag ccatacgagc acggaagccg
     3781 tgagaacggt tgcgcttcag tacagacggt tgaaaagtgc gtttcatggc gatttctacc
     3841 taaacttgaa taaattcaat ggctttattg gatatccgcc gaaaaatgaa acgatggaca
     3901 ccgaagccat gggtgattaa agaggccgga ttgtaataat tgtacacacc ggagtcaatt
     3961 ctctttcctt atttaccgcg cttttccgca ccttttcgca gggaaaatgt acgacctcac
     4021 accagtggaa accagcatgg cgcgccgggt ggaggattat acgggctgat gggtaaagcg
     4081 caaggatcgt cctggatctt tattagatcg attaagccaa tttttgtcta tggtcattaa
     4141 attttccaat atgcggcgta aatcgtgccc gcctcgcggc aggatcgttt acacttagcg
     4201 agttctggaa agtcctgtgg ataaataggg aaaatctgtg agaaacagaa gatctcttgc
     4261 gcagtttagg ctatgatccg cggtcccgat cgttttgcag gatcttgatc gggcatataa
     4321 ccgcagacag cggttcgtgc gtcaccctca agcagggtct tttcgacgta cgtcaacaat
     4381 catgaatgtt tcagccttag tcattatcga cttttgttcg agtggagtcc gccgtgtcac
     4441 tttcgctttg gcagcagtgt cttgcccgat tgcaggatga gttaccagcc acagaattca
     4501 gtatgtggat acgcccattg caggcggaac tgagcgataa cacgctggcc ctgtacgcgc
     4561 caaaccgttt tgtcctcgat tgggtacggg acaagtacct taataatatc aatggactgc
     4621 taaccagttt ctgcggagcg gatgccccac agctgcgttt tgaagtcggc accaaaccgg
     4681 tgacgcaaac gccacaagcg gcagtgacga gcaacgtcgc ggcccctgca caggtggcgc
     4741 aaacgcagcc gcaacgtgct gcgccttcta cgcgctcagg ttgggataac gtcccggccc
     4801 cggcagaacc gacctatcgt tctaacgtaa acgtcaaaca cacgtttgat aacttcgttg
     4861 aaggtaaatc taaccaactg gcgcgcgcgg cggcttgcca ggtggcggat aaccctggcg
     4921 gtgcctataa cccgttgttc ctttatggcg gcacgggtct gggtaaaact cacctgctgc
     4981 atgcggtggg taacggcatt atggcgcgca agccgaatgc caaagtggtt tatatgcact
     5041 ccgagcgctt tgttcaggac atggttaaag ccctgcaaaa caacgcgatc gaagagttta
     5101 aacgctacta ccgttccgta gatgcactgc tgatcgacga tattcagttt tttgctaata
     5161 aagaacgatc tcaggaagag tttttccaca ccttcaacgc cctgctggaa ggtaatcaac
     5221 agatcattct cacctcggat cgctatccga aagagatcaa cggcgttgag gatcgtttga
     5281 aatcccgctt cggttgggga ctgactgtgg cgatcgaacc gccagagctg gaaacccgtg
     5341 tggcgatcct gatgaaaaag gccgacgaaa atgacattcg tttgccgggt gaagtggcgt
     5401 tctttatcgc caagcgtcta cgatctaacg tacgtgagct ggaaggggcg ctgaaccgcg
     5461 ttattgctaa cgccaacttt accggacggg cgatcaccat cgacttcgtg cgtgaggcgc
     5521 tgcgcgactt gctggcattg caggaaaaac tggtcaccat cgacaatatt cagaagacgg
     5581 tggcggagta ctacaagatc aaagtcgcgg atctcctttc caagcgtcga tcccgctcgg
     5641 tggcgcgtcc gcgccagatg gcgatggcgc tagcgaaaga gctgactaac cacagtctgc
     5701 cagagattgg cgatgcgttt ggtgggcgtg accacacgac ggtgcttcat gcctgccgta
     5761 agatcgagca gttgcgtgaa gagagccacg atatcaaaga agatttttca aatttaatca
     5821 gaacattgtc atcgtaaacc tatgaaattt accgtagaac gtgagcattt attaaaaccg
     5881 ctacaacagg tgagcggtcc gttaggtggt cgtcctacgc taccgattct cggtaatctg
     5941 ctgttacagg ttgctgacgg tacgttgtcg ctgaccggta ctgatctcga gatggaaatg
     6001 gtggcacgcg ttgcgctggt tcagccacac gagccaggag cgacgaccgt tccggcgcgc
     6061 aaattctttg atatctgccg tggtctgcct gaaggcgcgg aaattgccgt gcagctggaa
     6121 ggtgaacgga tgctggtgcg ctccgggcgt agccgttttt cgctgtctac cctgccagcg
     6181 gcggatttcc cgaacctcga tgactggcag agcgaagtcg aatttaccct gccgcaggcg
     6241 acgatgaagc gtctgattga agcaacccag ttttcgatgg cgcatcagga tgttcgctat
     6301 tacttaaacg gtatgctgtt tgaaaccgaa ggtgaagaac tgcgcaccgt ggcaaccgac
     6361 ggccaccgtc tggcggtctg ttcaatgcca attggtcaat ctttgccaag ccattcggtg
     6421 atcgtaccgc gtaaaggcgt gattgaactg atgcgtatgc tcgacggcgg cgacaatctg
     6481 ctgcgtgtgc agattggcag caataacatt cgtgcccacg ttggcgactt tattttcacc
     6541 tcgaaactgg tggatggtcg cttcccggat taccgtcgcg ttctgccgaa gaacccggac
     6601 aaacatctgg aagcaggctg cgatctgctc aagcaggcgt ttgctcgcgc ggcgattctc
     6661 tctaacgaga aattccgcgg tgtgcgcctg tatgtcagcg aaaaccagct gaaaattact
     6721 gccaacaacc cggaacagga agaagcggaa gagatcctcg acgttaccta tagcggtgcg
     6781 gagatggaaa tcggcttcaa cgtcagctat gtgctggatg ttctgaacgc gctgaaatgc
     6841 gaaaacgtcc gcatgatgct gaccgattcg gtttccagcg tgcagattga agatgccgca
     6901 tcacagtcgg ctgcctatgt tgtcatgcca atgagactgt aatgtccctc acccgcttgt
     6961 tgatccgcga tttccgcaat attgaaaccg cggatctcgc tttatctccc ggctttaact
     7021 ttctggtagg tgccaacggc agtggcaaaa ccagcgtgct ggaagccatc tatacgctcg
     7081 gccatggtcg ggcgtttcgc agtttgcaga ttggtcgcgt cattcgccat gagcaggagg
     7141 catttgttct ccacgggcga ttacagggcg aagagcgcga gacggcgatt ggcttaacca
     7201 aagacaaaca gggcgacagc aaagtccgta ttgacggtac ggacgggcat aaagtcgcgg
     7261 aactggcgca cctgatgccg atgcagttga taacgccaga agggtttact ttactcaacg
     7321 gcggccccaa atacagaaga gcattcctcg actggggatg ctttcacaac gaacctggat
     7381 ttttcaccgc ctggagcaat ctcaagcgat tgctcaagca gcgcaatgcg gcgctgcgcc
     7441 aggtgactcg ttacgaacag ctacgcccgt gggataaaga actgatcccg ctggcggagc
     7501 aaatcagcac ctggcgcgcg gagtatagcg ccggtatcgc ggccgatatg gatgatacct
     7561 gtaagcaatt tctccctgag ttttctctga ctttctcttt ccagcgcggc tgggagaaag
     7621 agacagagta cgctgaggtg ctggaacgta attttgaacg cgatcgccag ctaacctaca
     7681 ccgcgcatgg cccgcataaa gcggacttac gcattcgcgc cgacggtgcg ccggtggaag
     7741 ataccttatc gcgtgggcag cttaagctgt tgatgtgcgc cttacgtctg gcgcaaggag
     7801 agttcctcac tcgtgaaagc gggcggcggt gtctctacct gatagatgat tttgcctctg
     7861 agcttgatga tgagcgtcgc gggttgcttg ccagccgctt aaaagcgacg caatcacagg
     7921 tctttgtcag cgcgatcagt gctgaacacg ttatagacat gtcggacgaa aattcgaaga
     7981 tgtttaccgt ggaaaagggt aaaataacag attaacccaa gtataaatga gcgagaaacg
     8041 ttgatgtcga attcttatga ctcctccagt atcaaagtcc tgaaagggct ggatgcggtg
     8101 cgtaagcgcc cgggtatgta tatcggcgac acggatgacg gcaccggtct gcaccacatg
     8161 gtattcgagg tggtagataa cgctatcgac gaagcgctcg cgggtcactg taaagaaatt
     8221 atcgtcacca ttcacgccga taactctgtc tctgtacagg atgacgggcg cggcattccg
     8281 accggtattc acccggaaga gggcgtatcg gcggcggaag tgatcatgac cgttctgcac
     8341 gcaggcggta aatttgacga taactcctat aaagtgtccg gcggtctgca cggcgttggt
     8401 gtttcggtag taaacgccct gtcgcaaaaa ctggagctgg ttattcagcg cgagggtaaa
     8461 attcaccgtc agatctacga acacggtgta ccgcaggctc cgctggcggt taccggcgag
     8521 actgaaaaaa ccggcaccat ggtgcgtttc tggcccagcc tcgaaacctt caccaatgtg
     8581 accgagttcg aatatgaaat tctggcgaaa cgtctgcgtg agttgtcgtt cctcaactcc
     8641 ggcgtttcca ttcgtctgcg cgacaagcgt gacggcaaag aagaccactt ccactatgaa
     8701 ggcggcatca aagcgttcgt tgaatatctg aacaagaaca aaacgccgat ccacccaaat
     8761 atcttctact tctccaccga aaaagacggt attggcgttg aagtggcgct gcagtggaac
     8821 gatggcttcc aggaaaacat ctactgcttt accaacaaca ttccgcagcg tgacggcggt
     8881 actcacctgg caggcttccg tgcggcgatg acccgtaccc tgaatgccta catggacaaa
     8941 gaaggctaca gcaaaaaagc caaagtcagc gccaccggtg acgatgcgcg tgaaggcctg
     9001 attgcggtcg tttccgtaaa agtgccggac ccgaaattct cctcccagac caaagacaaa
     9061 ctggtttctt ctgaggtgaa atcggcggtt gaacagcaga tgaacgaact gctggcggaa
     9121 tacctgctgg aaaacccaac tgatgcgaaa atcgtggttg gca
//
LOCUS       DBKELA010000136         9153 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.136, whole genome shotgun sequence.
ACCESSION   DBKELA010000136 DBKELA010000000
VERSION     DBKELA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.136"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..923
                     /locus_tag="ACUQR0_003601"
     CDS             <1..923
                     /locus_tag="ACUQR0_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9110477.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACUQR0_003602"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACUQR0_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HHV9110478.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACUQR0_003603"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACUQR0_003603"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HHV9110479.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACUQR0_003604"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACUQR0_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HHV9110480.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACUQR0_003605"
     CDS             4446..4805
                     /locus_tag="ACUQR0_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HHV9110481.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="ACUQR0_003606"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="ACUQR0_003606"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HHV9110482.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QNYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6236
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="ACUQR0_003607"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="ACUQR0_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HHV9110483.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="ACUQR0_003608"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="ACUQR0_003608"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HHV9110484.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2215 c         2469 g         2375 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatt ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgctacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DBKELA010000137         9014 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.137, whole genome shotgun sequence.
ACCESSION   DBKELA010000137 DBKELA010000000
VERSION     DBKELA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.137"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACUQR0_003609"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACUQR0_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HHV9110485.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACUQR0_003610"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACUQR0_003610"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HHV9110486.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACUQR0_003611"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACUQR0_003611"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HHV9110487.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACUQR0_003612"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACUQR0_003612"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HHV9110488.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="ACUQR0_003613"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="ACUQR0_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HHV9110489.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /gene="smg"
                     /locus_tag="ACUQR0_003614"
     CDS             5545..6018
                     /gene="smg"
                     /locus_tag="ACUQR0_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HHV9110490.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="ACUQR0_003615"
     CDS             6047..6589
                     /locus_tag="ACUQR0_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HHV9110491.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="ACUQR0_003616"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="ACUQR0_003616"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HHV9110492.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="ACUQR0_003617"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="ACUQR0_003617"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HHV9110493.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="ACUQR0_003618"
     CDS             7986..8243
                     /locus_tag="ACUQR0_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HHV9110494.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="ACUQR0_003619"
     CDS             complement(8219..8773)
                     /locus_tag="ACUQR0_003619"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HHV9110495.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2188 c         2247 g         2322 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DBKELA010000138         8989 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.138, whole genome shotgun sequence.
ACCESSION   DBKELA010000138 DBKELA010000000
VERSION     DBKELA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.138"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="ACUQR0_003620"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="ACUQR0_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="ACUQR0_003621"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="ACUQR0_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="ACUQR0_003622"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="ACUQR0_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="ACUQR0_003623"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="ACUQR0_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="ACUQR0_003624"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="ACUQR0_003624"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetA"
                     /protein_id="HHV9110496.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="ACUQR0_003625"
     CDS             8509..8952
                     /locus_tag="ACUQR0_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HHV9110497.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2356 a         2168 c         2167 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DBKELA010000139         8898 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.139, whole genome shotgun sequence.
ACCESSION   DBKELA010000139 DBKELA010000000
VERSION     DBKELA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.139"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..2887
                     /locus_tag="ACUQR0_003626"
                     /pseudo
     CDS             1..2887
                     /locus_tag="ACUQR0_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="ACUQR0_003627"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="ACUQR0_003627"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HHV9110498.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="ACUQR0_003628"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="ACUQR0_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="ACUQR0_003629"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="ACUQR0_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="ACUQR0_003630"
     CDS             complement(7280..8041)
                     /locus_tag="ACUQR0_003630"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HHV9110499.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="ACUQR0_003631"
     CDS             complement(8038..8697)
                     /locus_tag="ACUQR0_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HHV9110500.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2318 g         2049 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgta gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DBKELA010000140         8680 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.140, whole genome shotgun sequence.
ACCESSION   DBKELA010000140 DBKELA010000000
VERSION     DBKELA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.140"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(32..870)
                     /gene="aadA1"
                     /locus_tag="ACUQR0_003632"
                     /pseudo
     CDS             complement(32..870)
                     /gene="aadA1"
                     /locus_tag="ACUQR0_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(983..1813)
                     /locus_tag="ACUQR0_003633"
     CDS             complement(983..1813)
                     /locus_tag="ACUQR0_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HHV9110501.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2023..3036
                     /gene="intI1"
                     /locus_tag="ACUQR0_003634"
     CDS             2023..3036
                     /gene="intI1"
                     /locus_tag="ACUQR0_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HHV9110502.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3239..3289
                     /locus_tag="ACUQR0_003635"
                     /pseudo
     CDS             3239..3289
                     /locus_tag="ACUQR0_003635"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3389..3589
                     /locus_tag="ACUQR0_003636"
     CDS             3389..3589
                     /locus_tag="ACUQR0_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HHV9110503.1"
                     /translation="MNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQ
                     ARASTATETSVKPDACDSPPSG"
     gene            3715..4275
                     /locus_tag="ACUQR0_003637"
     CDS             3715..4275
                     /locus_tag="ACUQR0_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHV9110504.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4278..7244
                     /locus_tag="ACUQR0_003638"
     CDS             4278..7244
                     /locus_tag="ACUQR0_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HHV9110505.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7311..7688
                     /locus_tag="ACUQR0_003639"
     CDS             7311..7688
                     /locus_tag="ACUQR0_003639"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9110506.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7889..8548)
                     /locus_tag="ACUQR0_003640"
     CDS             complement(7889..8548)
                     /locus_tag="ACUQR0_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HHV9110507.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1930 a         2457 c         2325 g         1968 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggtttgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     2161 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     2221 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     2281 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     2341 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     2401 ggttttctgg aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg
     2461 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
     2521 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
     2581 agcctgcgcg agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc
     2641 cgcagcggcg ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc
     2701 tggccgtggt tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc
     2761 gtgcgtcgcc atcacatgta tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa
     2821 caagcaggca tcacgaagcc cgccacaccg cacaccctcc gccactcgtt cgcgacggcc
     2881 ttgctccgca gcggttacga cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc
     2941 tctacgacga tgatttacac gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg
     3001 cttgatgcgc tgccgcccct cactagtgag aggtagggca gcgcaagtca atcctggcgg
     3061 attcactacc cctgcgcgaa ggccatcggt gccgcatcga acggccggtt gcggaaagtc
     3121 ctccctgcgt ccgctgatgg ccggcagcag cccgtcgttg cctgatggat ccaacccctc
     3181 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacaat
     3241 ggaggtggta gccgagggtg tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg
     3301 ttgcgacacg gtgcagggtt tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg
     3361 cttcgtcaac caatggagga acaccaccat gaacgccaat gaaccgagca ccagttgctg
     3421 cgtgtgctgc aaggaaatcc cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt
     3481 ggagcatttc tgcgggctgg agtgctatca gcgcttccag gcgcgggcca gcactgcgac
     3541 cgaaaccagc gtcaaaccgg acgcttgtga ttcgccgccg tcaggttgag gcatacccta
     3601 acctgatgtc agatgccatg tgtaaattgc gtcaggatag gattgaattt tgaatttatt
     3661 gacatatctc gttgaaggtc atagagtctt ccctgacatt ttgcagggaa ttccatgact
     3721 ggacagcgca ttgggtatat cagggtcagc accttcgacc agaacccgga acggcaactg
     3781 gaaggcgtca aggttgatcg cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt
     3841 ccgcaactgg aagcgctgat aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc
     3901 atggatcgcc tggcgcgcaa tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa
     3961 cgcggcgtgc atatcgaatt cgtcaaggaa cacctcagtt ttactggcga agactctccg
     4021 atggcgaacc tgatgctctc ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc
     4081 cgcgagcgtc agcgcgaggg tattgcgctc gccaagcaac gcggggctta ccgtggcagg
     4141 aagaaatccc tgtcgtctga gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag
     4201 caaaagacca agcttgctcg tgaattcgga atcagtcgcg aaaccctgta tcaatacttg
     4261 agaacggatc agtaaatatg ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc
     4321 tgctggcgtt gccggactcc aaggacgacc tgatccgaca ttacacattc aacgataccg
     4381 acctctcgat catccgacag cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc
     4441 tctgttacct gcgctttccc ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct
     4501 tgttgaagct ggtcgccgac cagctcaagg tcggcgtcga aagctggaac gagtacggcc
     4561 agcgggagca gacccggcgc gagcacctga gcgagctgca aaccgtgttc ggtttccggc
     4621 ccttcaccat gagccattac cggcaggccg tccagatgct gaccgagctg gcgatgcaaa
     4681 ccgacaaagg catcgtgctg gccagcgcct tgatcgggca cctgcggcgg cagtcggtca
     4741 ttctgcccgc cctcaacgcc gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc
     4801 ggcgcatcta cgacgccttg gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg
     4861 atctgctcaa gcgccgggac aacggcaaga cgacctggtt ggcttggttg cgccagtctc
     4921 cggccaagcc aaattcgcgg catatgctgg aacacatcga acgcctcaag gcatggcagg
     4981 cactcgatct gcctaccggc atcgagcggc tggttcacca gaaccgcctg ctcaagattg
     5041 cccgcgaggg cggccagatg acacccgccg acctggccaa attcgagccg caacggcgct
     5101 acgccactct cgtggcgctg gccaccgagg gcatggccac cgtcaccgac gaaatcatcg
     5161 acctgcacga ccgcatcctg ggtaagctgt ttaacgctgc caagaataag catcagcagc
     5221 agttccaggc gtcaggcaag gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc
     5281 aggcgctgat cgacgccaag caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca
     5341 tgtcctggga ttcctttgcc gagagcgtca ccgaggcgca gaagctcgcg caacccgatg
     5401 acttcgattt cctgcatcgc atcggcgaga gctacgccac cctgcgccgc tatgcaccgg
     5461 aattccttgc cgtgctcaag ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca
     5521 ttgaggtgct gcgcggcatg aacaccgaca acgcccgcaa gctgccagcc gatgcaccga
     5581 ccggcttcat caagccgcgc tggcagaaac tggtgatgac cgacgccggc atcgaccggc
     5641 gctactacga actgtgcgcg ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct
     5701 gggtgcaggg ttcacgccag ttcaaggact tcgaggacta cctggtaccg cccgagaagt
     5761 tcaccagcct caagcagtcc agcgaattgc cgctggccgt ggccaccgac tgcgaacaat
     5821 atctgcatga gcggctgacg ctgctggaag cacaacttgc caccgtcaac cgcatggcgg
     5881 cagccaacga cctgccggat gccatcatca ccgagtcggg cttgaagatc acgccgctgg
     5941 atgcggcggt gcccgacacc gcgcaggcgc tgatagacca gacagccatg gtcctgccgc
     6001 acgtcaagat caccgaactg ctgctcgaag tcgatgagtg gacgggcttc acccggcact
     6061 tcacgcactt gaaatcgggc gatctggcca aggacaagaa cctgttgttg accacgatcc
     6121 tggccgacgc gatcaacctg ggcctgacca agatggccga gtcctgcccc ggcacgacct
     6181 acgcgaagct cgcttggctg caagcctggc atacccgcga cgaaacgtac tcgacagcgt
     6241 tggctgaact ggtcaacgct cagtttcggc atccctttgc cgggcactgg ggcgatggca
     6301 ccacatcatc atcggacgga cagaatttcc gaaccgctag caaggcaaag agcacggggc
     6361 acatcaaccc aaaatatggc agcagcccag gacggacttt ctacacccac atctccgacc
     6421 aatacgcgcc attccacacc aaggtggtca atgtcggcct gcgcgactca acctacgtgc
     6481 tcgacggcct gctgtaccac gaatccgacc tgcggatcga ggagcactac accgacacgg
     6541 cgggcttcac cgatcacgtc ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc
     6601 gcatccgcga cctgggcgac accaagctct acatcccgaa gggcgatgcc gcctatgacg
     6661 cgctcaagcc gatgatcggc ggcacgctca acatcaagca cgtccgcgcc cattgggacg
     6721 aaatcctgcg gctggccacc tcgatcaagc agggcacggt gacggcctcg ctgatgctca
     6781 ggaaactcgg cagctacccg cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc
     6841 gcatcgagcg cacgctgttc atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg
     6901 tgcatgccgg gctgaacaag ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca
     6961 accgccttgg tgaaatccgt gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc
     7021 tcaacctggt gacggcggcc atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc
     7081 atgcgttgcg cggcaatggt catgccgtcg atgactcgct attgcagtac ctgtcgccac
     7141 tcggctggga gcacatcaac ctgaccggtg attacctatg gcgcagcagc gccaagatcg
     7201 gcgcggggaa gttcaggccg ctacggcctc tgcaaccggc ttagcgtgct ttattttccg
     7261 ttttctgaga cgacccctat tatggcgaag gtatcattca ggaagcgttg atgaaggatt
     7321 atctctgtaa aaagttattc aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg
     7381 gcaataacat tactggcctg gcatgttgca atattcttta tccctcgccc cgatacagcg
     7441 gtcagctgca tattaaagag ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta
     7501 aagcgataat gcgctttata gcccggcttg cgcttgaaca ggaatgcctt agccttagct
     7561 ggaacgctga aaaatccaac cccggcgcta accgttttta tcaggctctg ggaggcagaa
     7621 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     7681 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     7741 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     7801 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     7861 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     7921 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     7981 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     8041 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     8101 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     8161 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     8221 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     8281 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     8341 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     8401 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     8461 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     8521 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     8581 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     8641 cgtgccgatc aacgtctcat tttcgccaaa agttggccca
//
LOCUS       DBKELA010000141         8642 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.141, whole genome shotgun sequence.
ACCESSION   DBKELA010000141 DBKELA010000000
VERSION     DBKELA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.141"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..91)
                     /locus_tag="ACUQR0_003641"
     CDS             complement(<1..91)
                     /locus_tag="ACUQR0_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110508.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPST"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="ACUQR0_003642"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="ACUQR0_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HHV9110509.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="ACUQR0_003643"
     CDS             complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="ACUQR0_003643"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HHV9110510.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="ACUQR0_003644"
     CDS             complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="ACUQR0_003644"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HHV9110511.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="ACUQR0_003645"
     CDS             complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="ACUQR0_003645"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HHV9110512.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5267..7813
                     /gene="cyaA"
                     /locus_tag="ACUQR0_003646"
     CDS             5267..7813
                     /gene="cyaA"
                     /locus_tag="ACUQR0_003646"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HHV9110513.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="ACUQR0_003647"
     CDS             complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="ACUQR0_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HHV9110514.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8221..>8642)
                     /locus_tag="ACUQR0_003648"
     CDS             complement(8221..>8642)
                     /locus_tag="ACUQR0_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HHV9110515.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1967 a         2235 c         2222 g         2218 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca ggttacgcac
     2101 gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg gaagatccat
     2161 cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg tggtggcatc
     2221 atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct ggcgataagt
     2281 ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc gaatattttc
     2341 gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg
     2401 aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga tacgccattc
     2461 gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat cgctgtcatt
     2521 atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc
     2581 gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg
     2641 aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac ttttcagcaa
     2701 cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga gtttcaccag
     2761 aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg tggcgatctt
     2821 ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg cctgcttaag
     2881 ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct cctgggcttt
     2941 ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat tgacggcctg
     3001 ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga tagccaccgc
     3061 gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag gttgtgacgt
     3121 ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt gttccgtcat
     3181 tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt
     3241 atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca aacgctcact
     3301 gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg ggatcagcga
     3361 ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct cgcgggattg
     3421 ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat aacattcaca
     3481 aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac gaccgccatt
     3541 gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta attgtagcaa
     3601 gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta cggtatgtag
     3661 cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc atttacgatc
     3721 ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag
     3781 atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc gacccggaga
     3841 aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac ggctcactaa
     3901 ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc ctctccgtta
     3961 tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc
     4021 cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg
     4081 tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc ggcgtagcta
     4141 ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc tgcggtaacg
     4201 cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg tgtatcatca
     4261 aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt
     4321 aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac ggcaagaatg
     4381 atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg
     4441 gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg taaactggac
     4501 gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt ggacacaaag
     4561 gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg cgggaattca
     4621 accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag gagcgcgact
     4681 tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt atcaagaatc
     4741 acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg gctcgccatc
     4801 aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt
     4861 cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc atcctaacat
     4921 ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat
     4981 gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga atttacagag
     5041 aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc agcaaggtgt
     5101 taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa
     5161 agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt accgttgatg
     5221 ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt acctctatat
     5281 tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc
     5341 tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt tgcactatca
     5401 ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt gcctttacac
     5461 gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt
     5521 acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg gcagcacctc
     5581 gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc aatcctggct
     5641 cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa gctgggccgc
     5701 ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc gtcataatga
     5761 aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc tgcttgacga
     5821 attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg
     5881 cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg gcgtgctgac
     5941 gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag agtactttgg
     6001 tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg tgctgaaaac
     6061 actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg cgaaagatat
     6121 caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat actgcatgat
     6181 gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc tggatttagt
     6241 acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg cctgtgtagg
     6301 ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg aagctcgtct
     6361 ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg cgcacaacga
     6421 gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc gtcgcaataa
     6481 ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac tgtatgccgc
     6541 gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac ccgatctctc
     6601 ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt caggttggta
     6661 tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc tggaatataa
     6721 ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga cctcgcgcac
     6781 ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg agatggtcgc
     6841 cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg cgctctacag
     6901 cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc cgacagctgc
     6961 gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca gctttggcga
     7021 gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt
     7081 gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg
     7141 caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt atagccagca
     7201 tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg
     7261 cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac
     7321 ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga acgccatcga
     7381 gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa
     7441 tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca tccagttctt
     7501 tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg aaagcaaccg
     7561 ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg
     7621 cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca acttcaacct
     7681 gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc cgttccgaac
     7741 aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc tattacagca
     7801 gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa tcttagcgga
     7861 aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc cagaaggttt
     7921 cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag ccgccctgtt
     7981 tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt ttgctgccgt
     8041 tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat
     8101 ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg cgatgaaatt
     8161 cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag cggcatgggg
     8221 tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat ccgtcaccgg
     8281 aataatgatg tctctgtttt gaacttcacg tacgttctcc tctctgacac gagttttcgc
     8341 cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa cattgcgatc
     8401 gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa tttgcaagct
     8461 ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa atgagcgatc
     8521 aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac caaatgtcga
     8581 taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa tcagatggaa
     8641 tt
//
LOCUS       DBKELA010000142         8412 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.142, whole genome shotgun sequence.
ACCESSION   DBKELA010000142 DBKELA010000000
VERSION     DBKELA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.142"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACUQR0_003649"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACUQR0_003649"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HHV9110516.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACUQR0_003650"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACUQR0_003650"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HHV9110517.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACUQR0_003651"
     CDS             3115..3399
                     /locus_tag="ACUQR0_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HHV9110518.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACUQR0_003652"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACUQR0_003652"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HHV9110519.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACUQR0_003653"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACUQR0_003653"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HHV9110520.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACUQR0_003654"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACUQR0_003654"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HHV9110521.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2139 g         2001 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gc
//
LOCUS       DBKELA010000143         8384 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.143, whole genome shotgun sequence.
ACCESSION   DBKELA010000143 DBKELA010000000
VERSION     DBKELA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.143"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..805
                     /gene="ttdA"
                     /locus_tag="ACUQR0_003655"
                     /pseudo
     CDS             1..805
                     /gene="ttdA"
                     /locus_tag="ACUQR0_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            802..1407
                     /gene="ttdB"
                     /locus_tag="ACUQR0_003656"
     CDS             802..1407
                     /gene="ttdB"
                     /locus_tag="ACUQR0_003656"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HHV9110522.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1455..2918
                     /gene="ttdT"
                     /locus_tag="ACUQR0_003657"
     CDS             1455..2918
                     /gene="ttdT"
                     /locus_tag="ACUQR0_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HHV9110523.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2961..3974)
                     /gene="tsaD"
                     /locus_tag="ACUQR0_003658"
     CDS             complement(2961..3974)
                     /gene="tsaD"
                     /locus_tag="ACUQR0_003658"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HHV9110524.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4212..4427
                     /gene="rpsU"
                     /locus_tag="ACUQR0_003659"
     CDS             4212..4427
                     /gene="rpsU"
                     /locus_tag="ACUQR0_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HHV9110525.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4538..6283
                     /gene="dnaG"
                     /locus_tag="ACUQR0_003660"
     CDS             4538..6283
                     /gene="dnaG"
                     /locus_tag="ACUQR0_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HHV9110526.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6478..8319
                     /gene="rpoD"
                     /locus_tag="ACUQR0_003661"
     CDS             6478..8319
                     /gene="rpoD"
                     /locus_tag="ACUQR0_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HHV9110527.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2022 a         2253 c         2249 g         1860 t
ORIGIN      
        1 ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg
       61 gtggataaac taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac
      121 catacgatgt tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac
      181 accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg
      241 caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat
      301 gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg
      361 gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc
      421 ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc
      481 gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta
      541 ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc
      601 attttgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc
      661 ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgaccgg caacagttca
      721 gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc gatctcacct ttgaaaatct
      781 gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat
      841 ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgc
      901 gacgtttgtc accgccgttt gatcgaactg aaacgtccga tcccttacga tctcaacggc
      961 aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gtgacaaatg ggagatggtc
     1021 tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tattgagcag
     1081 accggcgtaa aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc
     1141 cagaaattca aggcgttaca tgtgattttc ccagcaggct gcgcggtgct ggcggcaacc
     1201 caggtagaag agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg
     1261 gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac
     1321 ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc
     1381 tgcgagcacg tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc
     1441 gcaggcataa cacgatgaaa ccttccactg aatggtggcg atatcttgcg ccgctggcgg
     1501 tcatcgccat tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct
     1561 actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg
     1621 tggtggcgat ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc
     1681 ccgaacagct cgcccagcca ggctttaagt tcaccgccaa atccctctcc tgggcagttt
     1741 ctggtttttc taattcggtt atctggctca ttttcgccgc ctttatgttt ggcacaggct
     1801 atgaaaaaac cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atggggcatc
     1861 gcacgctgtt tctcggctat gcggtgatgt tctccgagct gatcctggca cctgtaacac
     1921 cgtccaactc ggcgcgtggt gcgggtatta tctatcccat catccgtaac ctgccaccgc
     1981 tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga
     2041 tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact
     2101 tgctgttaat tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt
     2161 tcctcgggat gttgccgctc agcattttac tggttctgct ggttccctgg ctggcttacg
     2221 tgctgtaccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac
     2281 tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg
     2341 cgctggtgct gtggattttc ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg
     2401 tggtggcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc agtaataaag
     2461 cggcatggaa cgttttcttc tggctggcct cgcttatcac cctcgcgacc ggactcaaca
     2521 ataccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagt ggttattcgc
     2581 cgacgatggt gatggtgacg ttgattgtgg tgttttatct actgcgctac tttttcgcca
     2641 gcgccacggc gtatacctgc gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc
     2701 cggaaatccc gctgccggtg ttctgcctga tggttggcgc ggcaattggt ctggggagca
     2761 ttcttacacc atacgccacc ggacccagtc cgatttacta cggtagtggt tatctgccaa
     2821 cggcggatta ctggcgactg ggggcgattt ttgggctgat attcctcgtg ttgctggtga
     2881 ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaaattg
     2941 ctctccggca cccaatagtt ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc
     3001 acgctaacac cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg
     3061 atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt
     3121 tttttcatca tttcagccag cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc
     3181 atgaccagtc gcttaaagcc cgtctgatcc aacgctcgct tacacttaat catcaacgta
     3241 tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg
     3301 ttgtcacgaa tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc
     3361 ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg agccgccatt
     3421 ttcgacagta acggtccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac
     3481 gcttcaccgg cggcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc
     3541 acgctgatta actgcgtatg accgccggac accagcagcg cgacaaacgg aaattccggc
     3601 gggttatctt ccagcatcgg cgctaacaga tgcccttcca tatggtgtac cgggattgcc
     3661 ggaacgttcc aggcaaacgc cagagaacgc cccacggttg cgccaaccag cagtgcgccg
     3721 actaatccag ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taaaccagac
     3781 tcctttagcg ccgcctggat caacggtacg gttttacgca catgatcgcg ggaggccagt
     3841 tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct
     3901 aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata
     3961 cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc
     4021 gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta
     4081 taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa
     4141 ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag
     4201 gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc
     4261 gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt
     4321 tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg
     4381 cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg
     4441 ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg
     4501 cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt
     4561 attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa
     4621 gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc
     4681 gtcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg
     4741 caacgcgatc gacttcctga tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga
     4801 gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat
     4861 cgagcgccat cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca
     4921 acaatcttta caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt
     4981 aagccacgag gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt
     5041 cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt
     5101 ggtcactaac gatcagggac gcagttacga tcgtttccgc gaacgggtga tgttccccat
     5161 tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc
     5221 caaatacctg aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct
     5281 ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat
     5341 ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc
     5401 aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta
     5461 tgacggcgac cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta
     5521 catgacagac ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac
     5581 gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact
     5641 ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg
     5701 cgcacgtttg agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg
     5761 aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga
     5821 acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg
     5881 cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt
     5941 cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact
     6001 ggtcaacact tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg
     6061 tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa
     6121 gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga
     6181 actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg
     6241 cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc
     6301 cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt
     6361 acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa
     6421 ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg
     6481 gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat
     6541 ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc
     6601 gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat
     6661 gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg
     6721 caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac
     6781 atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag
     6841 cgtattgaag acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc
     6901 acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg
     6961 atcaccggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc
     7021 ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc
     7081 gatgacgaca gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt
     7141 gcggaactac gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt
     7201 cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg
     7261 gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt
     7321 acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac
     7381 ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg
     7441 atgaacaagc cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg
     7501 caaaaactgc agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc
     7561 aaccgtcgta tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa
     7621 gcgaacttac gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc
     7681 cttgacctga ttcaggaagg taacatcggc ctgatgaaag cggttgataa attcgaatac
     7741 cgccgtggtt acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt
     7801 tctatcgcgg atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac
     7861 aaactcaacc gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa
     7921 gaactggctg aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc
     7981 aaagagccaa tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat
     8041 ttcatcgagg ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt
     8101 gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg
     8161 cgtttcggta tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac
     8221 gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg
     8281 agccgttctg aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt
     8341 tacgccgcac ccggcactag tctctctgca caaacgccac cttt
//
LOCUS       DBKELA010000144         8358 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.144, whole genome shotgun sequence.
ACCESSION   DBKELA010000144 DBKELA010000000
VERSION     DBKELA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.144"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            68..316
                     /locus_tag="ACUQR0_003662"
     CDS             68..316
                     /locus_tag="ACUQR0_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HHV9110528.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            381..749
                     /locus_tag="ACUQR0_003663"
     CDS             381..749
                     /locus_tag="ACUQR0_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HHV9110529.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            843..1496
                     /gene="nfsB"
                     /locus_tag="ACUQR0_003664"
     CDS             843..1496
                     /gene="nfsB"
                     /locus_tag="ACUQR0_003664"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HHV9110530.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1604..2851
                     /gene="ybdG"
                     /locus_tag="ACUQR0_003665"
     CDS             1604..2851
                     /gene="ybdG"
                     /locus_tag="ACUQR0_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HHV9110531.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="ACUQR0_003666"
     CDS             complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="ACUQR0_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HHV9110532.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="ACUQR0_003667"
     CDS             complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="ACUQR0_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HHV9110533.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="ACUQR0_003668"
                     /pseudo
     CDS             complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="ACUQR0_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2044 a         2326 c         2031 g         1957 t
ORIGIN      
        1 aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
       61 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
      121 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
      181 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
      241 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
      301 ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc
      361 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
      421 ctcttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
      481 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
      541 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
      601 ggtatcacat gaataaaaag cactggattt cggtgtattc cggcgaggaa atctccgaag
      661 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
      721 gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct atagcgcatt tttctcgctt
      781 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attacttaca catggagtct
      841 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
      901 aaaaacttac cccggaacag gccgagcaga tcaaaacgct actgcaatac agcccatcca
      961 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
     1021 ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg taaaatactt gatgcctcgc
     1081 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
     1141 accaggaaga tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
     1201 gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
     1261 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
     1321 acgcggtgcc catcgaaggt tttgacgccg caatcctcga tgcagaattt ggtctgaaag
     1381 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
     1441 acgctacgct gccgaaatct cgtctgccgc aaaacaccac cttaaccgaa gtgtaattct
     1501 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
     1561 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
     1621 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
     1681 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
     1741 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
     1801 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
     1861 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
     1921 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
     1981 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
     2041 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
     2101 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
     2161 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
     2221 ctggctggaa atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
     2281 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
     2341 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
     2401 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
     2461 taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa
     2521 tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac
     2581 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
     2641 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
     2701 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
     2761 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
     2821 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
     2881 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
     2941 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
     3001 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg
     3061 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgccgcgcga
     3121 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcaacgttgc taccaccagc
     3181 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
     3241 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
     3301 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
     3361 accccgctgt tatacaccga cagcgatgcc actagaatga cgaagttcag cgcagaagct
     3421 accacattgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
     3481 tccacccacg gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcaggatg
     3541 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
     3601 gctgtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
     3661 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
     3721 gctttctcgc cgccgtaacc agaaaacagc agccacaggc caaagccgat cataccgatg
     3781 atcgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc
     3841 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
     3901 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
     3961 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
     4021 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
     4081 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
     4141 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
     4201 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
     4261 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
     4321 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
     4381 acgaggtatc ctgctttaat tccgtacccg atgtcggtgc agccacatca gcttatacgc
     4441 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     4501 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     4561 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     4621 caacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggctcggc
     4681 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     4741 aagggcaata aagccggtac ccgtcgccac agaaagatga aagcccatcc accacagtaa
     4801 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     4861 ccgacggaat gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag
     4921 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     4981 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     5041 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     5101 cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg
     5161 cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc gccagctttg
     5221 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     5281 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     5341 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcagcaag
     5401 cgcagaagct acgcctggca ccgttcgcgc cacttcttca atttgctcag ccatcgcgtc
     5461 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     5521 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg
     5581 cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc gccactgatc
     5641 ctgtggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     5701 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     5761 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     5821 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     5881 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     5941 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcggattact
     6001 gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga tcactacgat
     6061 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     6121 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     6181 actgataaac agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     6241 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     6301 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     6361 cagcgacata atattggcat tcagcccctg gaagggcatg acaataaaag caatacacaa
     6421 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     6481 gcagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     6541 ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc tccacgcctt ccggcagact
     6601 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     6661 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     6721 ccggcgcatc ttcgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     6781 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct
     6841 ggcgcgcacc atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt
     6901 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     6961 aggatcgata accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac
     7021 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     7081 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     7141 ggcatctggt cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     7201 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     7261 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
     7321 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg
     7381 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt
     7441 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     7501 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     7561 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     7621 atcaggaaca ggccgccgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
     7681 gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca
     7741 tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc
     7801 agcatcggtt cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
     7861 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca
     7921 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagg
     7981 gtaaactgcg aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc
     8041 cacaccgggt ccataccctg aattcgcgat tacgcgcaat ccccctgaga accgccatga
     8101 acaagggttt catgatttgt gattttagtt tggtacgcaa tttggtacac aacactattt
     8161 tcacttcagc gggtagtcat caaactcacc ataacgagca tcgttgatga tgtatgtgat
     8221 caccacaaat atcacatctg gactagttcc gtcttcatgc gcaggtagac tggccccctg
     8281 aatctccaga caaccagtat cacttattta agtgatagtc ttaatactag tttttagact
     8341 tgtcattgga gaacagat
//
LOCUS       DBKELA010000145         8310 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.145, whole genome shotgun sequence.
ACCESSION   DBKELA010000145 DBKELA010000000
VERSION     DBKELA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.145"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            701..919
                     /locus_tag="ACUQR0_003669"
     CDS             701..919
                     /locus_tag="ACUQR0_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HHV9110534.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            1137..1379
                     /gene="yiiF"
                     /locus_tag="ACUQR0_003670"
     CDS             1137..1379
                     /gene="yiiF"
                     /locus_tag="ACUQR0_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HHV9110535.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1709..2638)
                     /gene="fdhE"
                     /locus_tag="ACUQR0_003671"
     CDS             complement(1709..2638)
                     /gene="fdhE"
                     /locus_tag="ACUQR0_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HHV9110536.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2635..3270)
                     /gene="fdoI"
                     /locus_tag="ACUQR0_003672"
     CDS             complement(2635..3270)
                     /gene="fdoI"
                     /locus_tag="ACUQR0_003672"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HHV9110537.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(3267..4169)
                     /gene="fdoH"
                     /locus_tag="ACUQR0_003673"
     CDS             complement(3267..4169)
                     /gene="fdoH"
                     /locus_tag="ACUQR0_003673"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HHV9110538.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(4182..7232)
                     /gene="fdnG"
                     /locus_tag="ACUQR0_003674"
     CDS             complement(4182..7232)
                     /gene="fdnG"
                     /locus_tag="ACUQR0_003674"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6645..6647),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HHV9110539.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7426..8259
                     /gene="fdhD"
                     /locus_tag="ACUQR0_003675"
     CDS             7426..8259
                     /gene="fdhD"
                     /locus_tag="ACUQR0_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HHV9110540.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1956 a         2114 c         2201 g         2039 t
ORIGIN      
        1 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
       61 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
      121 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
      181 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
      241 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
      301 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
      361 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
      421 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
      481 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
      541 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
      601 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
      661 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
      721 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
      781 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
      841 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
      901 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
      961 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     1021 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     1081 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     1141 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     1201 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     1261 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     1321 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     1381 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     1441 cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     1501 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     1561 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     1621 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     1681 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     1741 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     1801 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     1861 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     1921 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     1981 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     2041 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     2101 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     2161 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     2221 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     2281 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     2341 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     2401 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     2461 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     2521 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     2581 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     2641 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     2701 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     2761 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     2821 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     2881 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     2941 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     3001 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     3061 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     3121 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     3181 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     3241 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     3301 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     3361 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     3421 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     3481 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     3541 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     3601 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     3661 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     3721 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     3781 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     3841 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     3901 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     3961 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     4021 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     4081 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     4141 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     4201 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     4261 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     4321 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     4381 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     4441 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     4501 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     4561 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     4621 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     4681 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     4741 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     4801 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     4861 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     4921 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     4981 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     5041 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     5101 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     5161 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     5221 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     5281 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     5341 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     5401 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     5461 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     5521 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     5581 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     5641 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     5701 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     5761 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     5821 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     5881 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     5941 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     6001 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     6061 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     6121 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     6181 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     6241 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     6301 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     6361 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     6421 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     6481 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     6541 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     6601 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     6661 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     6721 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     6781 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     6841 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     6901 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     6961 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     7021 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     7081 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     7141 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     7201 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     7261 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     7321 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     7381 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     7441 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     7501 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     7561 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     7621 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     7681 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     7741 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     7801 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     7861 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     7921 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     7981 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     8041 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     8101 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     8161 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     8221 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     8281 atattaaatt gcaggactta ttcatttcgt
//
LOCUS       DBKELA010000146         8179 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.146, whole genome shotgun sequence.
ACCESSION   DBKELA010000146 DBKELA010000000
VERSION     DBKELA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.146"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="ACUQR0_003676"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="ACUQR0_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HHV9110541.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="ACUQR0_003677"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="ACUQR0_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HHV9110542.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="ACUQR0_003678"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="ACUQR0_003678"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HHV9110543.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="ACUQR0_003679"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="ACUQR0_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="ACUQR0_003680"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="ACUQR0_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HHV9110544.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="ACUQR0_003681"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="ACUQR0_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HHV9110545.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(5893..6162)
                     /locus_tag="ACUQR0_003682"
     CDS             complement(5893..6162)
                     /locus_tag="ACUQR0_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9110546.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            6219..6887
                     /locus_tag="ACUQR0_003683"
     CDS             6219..6887
                     /locus_tag="ACUQR0_003683"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHV9110547.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7203..>8179
                     /locus_tag="ACUQR0_003684"
     CDS             7203..>8179
                     /locus_tag="ACUQR0_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NEL-type E3 ubiquitin ligase domain-containing
                     protein"
                     /protein_id="HHV9110548.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELR"
BASE COUNT         2354 a         1858 c         1649 g         2318 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttatataaaa tcggtaacgg ctggaaatca ttcaatactc
     5821 gcactatcga aagtttacca gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt
     5881 tttctttttc gcttacattg tgtctggttc ttccggccac tcaatatcag gtgcagttga
     5941 tgtatcaaca cggttcagca acacccgata cttcttccag gcttccagca acgagttttc
     6001 ttcctccgtt gcgatctcca gatctacagc atcctgaagt ggcgcaatat gctcactgaa
     6061 tccctggatg tagaactgtg tggtgacggt cttccagcca ttcggctcct gctgtatcga
     6121 agcataccag gctatttcaa tatcgctatg ctgcggcagc atttaacccc ttgtaattca
     6181 tcgccataat tgatttaatt cacaaataaa actataatat ggtgaaatta atgaaaaaaa
     6241 acacagatga tggggctaaa atttacacac cacttaccct aaagctttat gactggtggg
     6301 ttttgggagt atcaaatcgg cttgcatggg gatgtcctac aaaggaacac cttcttccac
     6361 actttctgga acatttaggt aacaaccatc tggatattgg tgttggaact gggttttacc
     6421 ttactcacgt acctgagagt agtctgatat ctttaatgga tttgaacgaa gctagcctga
     6481 acgcggcatc tacaagggct ggggaatcaa aaattaaaca taaaattagc catgatgttt
     6541 ttgaacctta tcctgcggcg ttacatggtc aatttgattc catttccatg ttttaccttc
     6601 ttcactgcct gcctggaaat atatctacaa aaagctgtgt aatacgcaat gcggcgcagg
     6661 ccttaactga tgatggaact ctatacggag ccacaattct tggcgatgga gttgtgcaca
     6721 atagcttcgg tcaaaaactg atgcgcattt acaatcagaa aggcatcttt tcaaacacaa
     6781 aagattccga agaaggctta acacatatac tctcagagca tttcgagaat gttaaaacca
     6841 aggttcaagg tactgtagta atgttttccg cttcagggaa aaaatagcat ccaaccgcag
     6901 cacgttcttg cttaagacgt gctacggttt cctttacgat ttggggttgc gactttacct
     6961 ctattgataa tgcattccgg cagaacgttc aaatatgaac gtacgatatt taactaaccg
     7021 aaaaacaaaa tatagcacag gcgagacatt taccatctaa gaaaatagta tcgttttttt
     7081 acagttaaat caacatcact tccttaaaat gaaaacaata aatttcaatc ctgaaacatt
     7141 ttatttcagc ttattattcc ccagtatcac attttatcag gatatccttc tgcgaggtta
     7201 ttatgcttcc tgtaaataat ccccccctat ccactggaaa cgtctctttt tacagaacta
     7261 catcaatcga caatgttcac aataattatc tctccgaatg ggttgaatgg actaaaaaca
     7321 gcatttccgg agaaaacagg gaaactgctt ttacccggct ccaattatgt ctggagaaca
     7381 gtgaaacatc gttggactta tcttgtttag gtctcagatc tctaccacga ttgcctgaca
     7441 atcttgatga aattaatgta agcaataacc aactatcaat gctccccgag ctaccaaggg
     7501 cattgaaaga gctgaatgca agcagtaatc aattatctgc acttcctgaa ttaccagtgt
     7561 cgctggaata tataaatgtg agtgataacc atttgttcgc acttcctgaa ttacctgcgt
     7621 cactagaata tattaatgta agtgacaatc acctgtctgt acttccgagg ttaccaatgt
     7681 cattggaatt acttgatgca gccagaaatg ctttggaagt aataccagat tttccagaaa
     7741 gagatgatca tattataaga atattctggc ttaatcagaa ccggatcacg gcaattccgg
     7801 aaagcatact tggcctcagt tctgatagcg ttgtcaatct tagagaaaat caactatctc
     7861 ccagaataat gcaaactttg ttacaacaaa ccgcccaacc ggactaccac ggcccacgga
     7921 tttacttctc catgagtgac ggacaacaga atacactcca tcgccccctg gctgatgccg
     7981 tgacagcatg gttcccggaa aacaaacaat ctgatgtatc acagatatgg catgcttttg
     8041 aacatgaaga gcacgccaac accttttccg cgttccttga ccgcctttcc gataccgtct
     8101 ctgcacgcaa tacctccgga ttccgtgaac aggtcgctgc atggctggaa aaactcagtg
     8161 cctctgcgga gcttcgaca
//
LOCUS       DBKELA010000147         7916 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.147, whole genome shotgun sequence.
ACCESSION   DBKELA010000147 DBKELA010000000
VERSION     DBKELA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.147"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            237..740
                     /gene="dps"
                     /locus_tag="ACUQR0_003685"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="ACUQR0_003685"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HHV9110549.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMPGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="ACUQR0_003686"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="ACUQR0_003686"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HHV9110550.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="ACUQR0_003687"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="ACUQR0_003687"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HHV9110551.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="ACUQR0_003688"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="ACUQR0_003688"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HHV9110552.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="ACUQR0_003689"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="ACUQR0_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="ACUQR0_003690"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="ACUQR0_003690"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HHV9110553.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /gene="mcbA"
                     /locus_tag="ACUQR0_003691"
     CDS             7091..7351
                     /gene="mcbA"
                     /locus_tag="ACUQR0_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HHV9110554.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7916
                     /locus_tag="ACUQR0_003692"
     CDS             7616..>7916
                     /locus_tag="ACUQR0_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HHV9110555.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1934 a         2026 c         1977 g         1979 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcc cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DBKELA010000148         7856 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.148, whole genome shotgun sequence.
ACCESSION   DBKELA010000148 DBKELA010000000
VERSION     DBKELA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.148"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            54..587
                     /gene="ymdB"
                     /locus_tag="ACUQR0_003693"
     CDS             54..587
                     /gene="ymdB"
                     /locus_tag="ACUQR0_003693"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HHV9110556.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            529..2010
                     /gene="clsC"
                     /locus_tag="ACUQR0_003694"
     CDS             529..2010
                     /gene="clsC"
                     /locus_tag="ACUQR0_003694"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HHV9110557.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="ACUQR0_003695"
     CDS             complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="ACUQR0_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HHV9110558.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3569..5104
                     /gene="mdoG"
                     /locus_tag="ACUQR0_003696"
     CDS             3569..5104
                     /gene="mdoG"
                     /locus_tag="ACUQR0_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HHV9110559.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5097..7640
                     /gene="mdoH"
                     /locus_tag="ACUQR0_003697"
     CDS             5097..7640
                     /gene="mdoH"
                     /locus_tag="ACUQR0_003697"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HHV9110560.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1939 a         1882 c         2078 g         1957 t
ORIGIN      
        1 taaccaaaaa gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa
       61 cgcgtattca tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg
      121 cggctaatcc gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg
      181 gtccggccct gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg
      241 ggcatgccgt tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg
      301 ggccagtctg gcgtggtggg gaacaaaacg aagaccagct tttgcaggat gcctatctga
      361 atagcctacg actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta
      421 ctggggttta tagttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag
      481 aatttattac ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa
      541 acgcccacct ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg
      601 gcgagcgcgg tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg
      661 gagaaaagtc tggatgcgtt tgccgcccgg tatcgtctag ccgaaatggc agagcatacg
      721 ctggatgttc agtattacat ctggcaggac gatatgtcgg ggcggttact gttttccgcc
      781 ctgttagccg cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg
      841 cccggacttg acgacatttt gcgcttgctt gacagtcatc cacgcattga agtccggctt
      901 tttaatcctt tctcgtttcg cttgctacgt ccgcttggat atatcaccga cttttcccgt
      961 ctcaatcgcc ggatgcacaa taaaagtttc actgtcgatg gcgtggttac cctagtggga
     1021 ggacgaaata ttggtgatgc ctattttggt gcaggggaag agccactttt ttcggattta
     1081 gatgtcatgg caataggccc cgtggtagag gatgttgccg atgatttcgc ccgctactgg
     1141 tattgcaaat cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg
     1201 gatcgcatcg agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttatttacgc
     1261 aaaatggaat ccagtccatt tataaatcat ctggttgatg gaacgttgcc gcttatctgg
     1321 gcgaagacac gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat
     1381 tcactgttac cgcagcgcct gttcgatatc atgggctcac ccagtgaacg catcgatatt
     1441 atctcttcct attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga
     1501 aaaggggtaa agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc
     1561 catgccggat acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa
     1621 ctcaagccga cgcgtgaaca aagcagtacg ttacacgatc gcggcataac cggtaattcc
     1681 ggtgccagcc tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct
     1741 ttcaatttcg atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc
     1801 gaaacgctgg cacagttaat tgataaacgc tttattcaga gccagtacga tgcggcctgg
     1861 cagctccgtc tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag
     1921 gttattctca aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg
     1981 tcgatattgc ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt
     2041 tctcacgctt aacaaccggt ttaccagaga acaaaaactt cagtaacggg atgcgtaaat
     2101 gaatttcata cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa
     2161 gccagttgga ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt
     2221 gaaccagata gataaacagc gatgcgttaa caaaataagt cacccgcgct gactggaagt
     2281 taagcaaacg gtggccaaag gagaagacca cattcaccat ccacaggccg aggaccatgg
     2341 tgatcaccga ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta
     2401 aataagcgac aaacgccaat gctgcagcga gggtacagcc acgagacggc gtggtaaaca
     2461 aggctttaag atgagggaaa atgaaagcca gtgcgccgag gataaagaac ggtaaataaa
     2521 atagcgtttg catgacaata aaattgaaca tgccattact cagaatgggt ggatacacaa
     2581 taaaaatcgt tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata
     2641 gttttaccat cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc
     2701 gcttaaatat ccatacacac agcgtcgtca tgactactaa caccagtaaa aaccacaggt
     2761 gtgatattaa ttcccaggcc aacgtattat atttgtcata caatgatagc ccaggccaac
     2821 tttccgcttt ccctttgaca tattgcagca taataaattg cggtaatgtc agtaggggga
     2881 tggctgttaa catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg
     2941 gatagcgtaa aaaaagcatg taggaaaagt atccggatat aacgaaaaat acctgcatgc
     3001 ggaacgagtg gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg gcgctattca
     3061 catgccatgt atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca
     3121 gccaggcgcg gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg
     3181 gttaactaat ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc
     3241 catagcgaca ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc
     3301 atcgctacca ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aatttgctgg
     3361 caaaccctac acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt
     3421 tgcaatcagg tgcttaatcc atgagccagc gtgctgaacg ataccgggat tcagttgtcg
     3481 gaatggctgg ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg
     3541 cttactaatt atgaaacata aactacaaat gatgaaaatg cgttggttga gtgctgcagt
     3601 aatgttaacc ctgtatacat cttcaagctg ggctttcagt attgatgatg tcgcaaagca
     3661 agctcaatcc ttagccggga aaggctatga ggcgcctaaa agcaacttgc cctccgtttt
     3721 ccgcgacatg aaatacgcgg actatcagca gatccagttt aatcatgaca aagcgtactg
     3781 gaacaatctg aagaccccat tcaaactcga gttctaccat cagggtatgt acttcgatac
     3841 cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat acagcccgga
     3901 ttatttcact ttcggcgatg ttcagcatga caaagacacg gtaaaagacc ttggttttgc
     3961 cggttttaaa gtgctttacc cgatcaacag caaagataaa aacgatgaaa tcgtcagcat
     4021 gctcggggcc agctatttcc gcgtgattgg tgcaggtcag gtttatggcc tttctgcccg
     4081 cggcctagca attgataccg ctttgccatc gggtgaagaa tttccacgct tcaaagagtt
     4141 ctggatcgag cgtccaaaac cgactgataa acgtttaacc atttatgcat tgcttgactc
     4201 gccgcgtgcg acaggtgctt acaaattcgt ggttatgcca gggcgtgaca cggttgtgga
     4261 tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg caccgttaac
     4321 cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca aataactatc gtccggagtt
     4381 gcacgactct aacggtctgt ctatccatgc tggtaatggc gaatggatct ggcgtccgtt
     4441 gaataacccg aaacatttag cggtcagcag cttctccatg gaaaacccgc gaggctttgg
     4501 tctgttgcaa cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc gttacgatct
     4561 ccgtccaagc gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg ttgagctggt
     4621 ggaaattcca accaacgatg aaaccaacga taacatcgtc gcttactgga cgccggatca
     4681 gctgccggag ccgggtaaag agatgaactt taaatacacc atcaccttca gccgtgatga
     4741 agacaaactg catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt caacggggga
     4801 tgtgaagcag tcgaacctga ttcgccagcc tgacggtact atcgcctttg tggtcgattt
     4861 taccggcgca gagatgaaaa aactgccaga ggataccccg gtcacagcgc aaaccagcat
     4921 tggtgataat ggtgagatag ttgaaagcac ggtgcgctat aacccggtta ccaaaggctg
     4981 gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa tgcgtgctgc
     5041 gctggtgaat gccgatcaga cgttgagtga aacctggagc taccagttac ctgccaatga
     5101 ataagacaac tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa gcagcattgc
     5161 cgaagactga tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc acctgggcgc
     5221 gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc tggccagatt
     5281 cacttgctga tggacagtta attaaagacg acgaagggcg cgatcagctt aaggcgatgc
     5341 cagaagcaaa acgctcctcg atgtttcccg acccgtggcg taccaacccg gtaggccgtt
     5401 tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt ttgaccaaag
     5461 aagagcagga gagcgagcaa aagtggcgta ccgttggtac catccgccgt tacattctgt
     5521 tgatcctgac gctcgcgcaa actgttgtcg cgacctggta tatgaagacc attcttcctt
     5581 atcagggttg ggcgctgatt aatcctatgg atatggttgg tcaggatttg tgggtttcct
     5641 ttatgcagct tctgccttat atgctgcaaa ccggtatcct gatccttttt gcggtactgt
     5701 tctgttgggt gtccgccgga ttctggacgg cgttgatggg cttcctgcaa ctgcttattg
     5761 gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc
     5821 atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct
     5881 tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacacttt gatgtctaca
     5941 ttctgagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc
     6001 ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt cgccgcgtga
     6061 agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca
     6121 tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttatgcggc ctggtgcgct
     6181 tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggtatgg
     6241 atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag
     6301 ccggtttgca cttctggcaa cttggcgagt cgcactactg gggacataac gcgattatcc
     6361 gcgtgaaacc atttatcgag cactgcgcac tggctccgct gccgggcgaa ggttcctttg
     6421 ccggttcaat cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt gcaggttggg
     6481 gggtctggat tgcttacgat ctcccgggtt cttatgaaga attgccgcct aacttgcttg
     6541 atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc
     6601 tggttaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc
     6661 tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtgcatg
     6721 cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggtg tggccgcagt
     6781 ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga
     6841 agttattgag cattttgctt atctggtgca aaggaacgaa agaatacggc ggcttctggc
     6901 gcgttacatt atcgttgctg ctggaagtgc tgttttccgt gctgctggct ccggtacgca
     6961 tgctgttcca tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg gtgtggaatt
     7021 caccgcagcg tgatgatgac tccacttcat ggggcgaagc gttcaaacgc cacggctcac
     7081 agctgctgtt ggggttagtg tgggctgttg ggatggcgtg gctggatctg cgtttcctgt
     7141 tctggctggc accgattgtc ttctcgttga tcctgtcacc gtttgtttcg gtgatttcca
     7201 gccgtgccac cgttggtctg cgcaccaaac gctggaaact gttcctgatc ccggaagagt
     7261 attcaccgcc gcaggtgctg gttgataccg atcggttcct tgagatgaat cgccaacgct
     7321 cccttgacga tggctttatg cacgcagtgt ttaacccgtc atttaacgct ctggcaaccg
     7381 caatggcgac agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt gaccgccacg
     7441 ttgaacaggc gcttaacgag acgccagaga agctgaatcg cgatcgtcgc ctggtgctgc
     7501 taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc cctgaaagat
     7561 attcttcatg ggtgagttat tacgaaggga taaagctcaa tccactggca ttgcgtaaac
     7621 cggatgcggc ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat caggcctaca
     7681 tcgttcctgc aatttattga ttttgcaaga ttttgtaggt cggataaggc gttcacgcca
     7741 catccggcaa aaacaacgag ccaataaaaa taccggcgtt atgccgtaat gccagtcagt
     7801 taagcaactg actggctctt tttcggggct gtggggtatt tccagggcct ctcctt
//
LOCUS       DBKELA010000149         7838 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.149, whole genome shotgun sequence.
ACCESSION   DBKELA010000149 DBKELA010000000
VERSION     DBKELA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.149"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            55..1388
                     /gene="yjeM"
                     /locus_tag="ACUQR0_003698"
                     /pseudo
     CDS             55..1388
                     /gene="yjeM"
                     /locus_tag="ACUQR0_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="ACUQR0_003699"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="ACUQR0_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HHV9110561.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="asd"
                     /locus_tag="ACUQR0_003700"
     CDS             complement(4872..5840)
                     /gene="asd"
                     /locus_tag="ACUQR0_003700"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HHV9110562.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="ACUQR0_003701"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="ACUQR0_003701"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HHV9110563.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="ACUQR0_003702"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="ACUQR0_003702"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HHV9110564.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(7642..>7838)
                     /locus_tag="ACUQR0_003703"
     CDS             complement(7642..>7838)
                     /locus_tag="ACUQR0_003703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110565.1"
                     /translation="LLNFAFVIFAFLRRYGRRIIREIFPSATPQKSFFCVFISSAEII
                     SKTFDLASKSRRRKWIPALT"
BASE COUNT         1782 a         2038 c         2110 g         1908 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagta
//
LOCUS       DBKELA010000150         7769 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.150, whole genome shotgun sequence.
ACCESSION   DBKELA010000150 DBKELA010000000
VERSION     DBKELA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.150"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..306
                     /locus_tag="ACUQR0_003704"
     CDS             <1..306
                     /locus_tag="ACUQR0_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HHV9110566.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2206
                     /locus_tag="ACUQR0_003705"
     CDS             278..2206
                     /locus_tag="ACUQR0_003705"
                     /EC_number="3.1.21.4"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HHV9110567.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2190..2393
                     /locus_tag="ACUQR0_003706"
     CDS             2190..2393
                     /locus_tag="ACUQR0_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HHV9110568.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2390..3983
                     /locus_tag="ACUQR0_003707"
                     /pseudo
     CDS             2390..3983
                     /locus_tag="ACUQR0_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3973..5478
                     /locus_tag="ACUQR0_003708"
     CDS             3973..5478
                     /locus_tag="ACUQR0_003708"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HHV9110569.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5515..5862
                     /locus_tag="ACUQR0_003709"
     CDS             5515..5862
                     /locus_tag="ACUQR0_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HHV9110570.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5920..6948
                     /locus_tag="ACUQR0_003710"
     CDS             5920..6948
                     /locus_tag="ACUQR0_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HHV9110571.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7000..7386
                     /gene="gpFI"
                     /locus_tag="ACUQR0_003711"
     CDS             7000..7386
                     /gene="gpFI"
                     /locus_tag="ACUQR0_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HHV9110572.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7398..>7769
                     /locus_tag="ACUQR0_003712"
     CDS             7398..>7769
                     /locus_tag="ACUQR0_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HHV9110573.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEG"
BASE COUNT         1793 a         1815 c         2411 g         1750 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc cgtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgcgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca cctgggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtgttttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgctggcgg catcccgcaa ggatgcaact gcacaaccga cccttgaaga
     2161 actggcagcg aagctgtccg gaggagtgaa tggttacagt cgctgaactg caggcgctgc
     2221 gtcaggcgcg ccttgattta ttaaccggta aacgggttgt gtcggtacag aaagacggtc
     2281 gcaggatcga atatacggca gcgtccctga atgagcttaa ccgtgcgatc aacgatgcgg
     2341 agtcggttct gggtacgacc cgacgccgcc gaccgctggg agtgaggtta tgaaacgaac
     2401 gcctgtcctg attgatgtga acggcgttcc gcttcgtgag agtctcagct acaacggagg
     2461 cggcgcagga tttggcgggc aaatggcaga gtggttacca ccggcgcaga gtgccgatgc
     2521 ggccctgttg cctgcattgc gtctggggaa tgcccgggca gatgatctgg tgcgcaataa
     2581 cggtatcgcg gctaatgcgg tggccctgca taaggatcat atcgtcgggc acatgtttct
     2641 tatcagctac cgtctgaact ggcgttggct ggggatgcgg gagaccacgg caaaaagttt
     2701 tgtcggggag gtggaagcag cctggtcgga atacgcagaa ggaatgtttg gcgagatcga
     2761 cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa ggggtgggcg tgcatgcctt
     2821 taacggcgaa atctttgtgc agccggtctg ggatacggaa accacgcagt tattccgtac
     2881 gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca ggacacggta tggggaacca
     2941 ctttctgcgg gccggggttg aggtcgatcg gtatgggcgg gcgattgcgt accatatctg
     3001 tgaggatgat tttccgttct ctggtgccgg acgctgggag cggatcccgc gtgagcttcc
     3061 caccgggcgt ccggccatgc tgcatatttt tgagccggtg gaggacgggc agacccgtgg
     3121 ggccaatcag ttttacagcg taatggagcg gatgaagatg ctggactccc tgcaggccac
     3181 gcagcttcag tcggccattg tgaaggcgat gtatgcagcc acaatcgaaa gtgaacttga
     3241 taccgaaaag gcatttgaat atatcgcggg ggcaccgcaa ggtcagcagg ataatccgct
     3301 tattaatatt ctggagaagt tcacccgctg gtatgacacg aataacgtga cgctgggcgg
     3361 tgttaaaatt ccgcaccttt tttcctggcg atgcgctgaa cctgcagaca gcgcaggatt
     3421 cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatattgca gccggtcttg
     3481 gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttactca agtgcccgtg
     3541 cctccgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt gcggcccggc
     3601 tggccacgca gatgttttcc tgctggctgg aagaggcgct tcttcggggg attattcgtc
     3661 cgccacgggc gcgttttgat ttttaccagg cgcgttcagc ctggtcacgg gcagagtgga
     3721 ttggctccgg aagaatggcc attgacgggc tcaaggaggt ccaggaatcg gtgatgcgca
     3781 ttgaggccgg actgagcacg tatgagaaag aactggcact gatgggcgaa gattatcagg
     3841 acattttccg ccagcaggtc agggaatccg cagagcgcga aaaagccgga ctctcacgtc
     3901 cggtgtggat agcccaggcg tatcagcagc agatagcgga gagtcgcagg ccggaagagg
     3961 agacaacatc acgtgagacg taatctttca catattatcg ctgcggcatt caatgagccg
     4021 ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg tgagatgggc
     4081 gcagcaagcc tttcggtacc gcaacagcag gtacagcttg acgctcccgg gatgctggct
     4141 gaaacggacg agtacatggc cggaggcaaa cgacctgccc gtgtttaccg ggtggtgaac
     4201 ggtattgctg tactgccggt gaccggcacg ctggtacacc ggctgggtgg catgcggcca
     4261 ttttccggaa tgactggcta tgacggcatt gtcgcctgtc ttcagcaggc aatggcggac
     4321 actcaggtac ggggcgtact gctggacatt gacagtcctg gcgggcaggc caccggcgcg
     4381 tttgactgcg ctgacatgat ttaccgtctc cggcagctga agcctgtctg ggcactgtgc
     4441 aatgacacgg cctgttcagc cgccatgtta ctggcatcgg cctgctcccg acggctggtt
     4501 acccagacat cccgtatcgg ctccattggg gtgatgatgg gccatgtcag ctatgccggt
     4561 catctggcac aggccggtgt ggatatcacg ctgatttacg ccggatcgca caaggtggat
     4621 ggcaatcagt ttgaatccct gccagcaaag gttcgccagg acatgcagca gcgcattgat
     4681 gcggcgcgcc ggatgtttgc tgaaaaagtg gcgatgtata ccggtttatc tgtggatgcg
     4741 gttatgggaa cagaggccgc cgtttttgaa ggtcagtccg gtattgaggc cgaactggcg
     4801 gatgaattaa tcaatgcgtc ggacgccatc agcgtgatgg ctgcgacgct gaacacacac
     4861 gatacaggag gcactatgcc gcaattaact gcaacggagg ctgccgcgca ggagaaccag
     4921 cgagtgatgg ggatcctgac gtgtcaggaa gcgaaaggac gtgaacaact ggccacgatg
     4981 ctggcagggc aacagggtat gagcgttgag caggcccggg cgattctggc cgtggcggca
     5041 ccgcaacaat ctggtgtatc tgcgcagagt gaagccgatc gcattatggc atgtgaagag
     5101 gcgaaaggac gtgaacaact ggcggcaacg ctggcggcga tgccggagat gacggtggaa
     5161 aaagcccgtc cgattctggc tgcttcaccg caggcgaatg ccggaccctc acttcgtgat
     5221 cagattatgg ccctggatga ggcaaaaggg gcagaggcgc aggctgaaaa actggcggcc
     5281 tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg cggcagcctc aggcagggca
     5341 gaaccggttt ctgcatccac aaccgccatg tttgaacatt tcatggcgaa ccattcaccg
     5401 gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga cgtgaaaatg
     5461 ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttta caatatggta
     5521 acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa tgatccggcc
     5581 cataccgcca caggcagcag cgggatttcc tcggcaacac cggctctgac gcccctgatg
     5641 ctggatgagg ataccgggaa actggtggtc tgggacggac agaaggcagg ccatgtggtt
     5701 ggcatactgg tactgccgct tgaaggtaca gagacggtgc tgacgtatta caagtcgggg
     5761 acctttgcga cggaggccat ccactggcct gagagagtgg atacacacaa aaaggcgaat
     5821 gcctttgccg gcagtgccct gagtcacgca gcgctgccgt aacattttat caggtcactg
     5881 cggtagcctg actgatttct gaatgaaagg aactgattta tgggattgtt tacgacccgc
     5941 cagttactcg gttataccga acaaaaagtt aaatttcgtg cgctgtttct ggagctgttt
     6001 ttccgccgta cggtgaattt ccataccgaa gaggtaatgc tggacaaaat taccggaaaa
     6061 acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aggtgttgcg tcaccgtggc
     6121 ggtgaaactc gtgtactgcg cccgggctac gttaagccga aacacgaatt taattaccag
     6181 caggcggtgg agcgtcttcc cggtgaagat ccggcccagc tgaacgaccc ggcttaccgt
     6241 cgcctgcgta tcatcaccga taacctcaaa caggaagagc acgccattgt ccaggtggaa
     6301 gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg agagcagttt
     6361 gaaactgttg aggtcgattt tggccggtcg gtgggaaata acattacgca ggcaggaggg
     6421 acggagtgga gcgctcagga ccgcgatacc tttgatccga cgcatgatct ggatgcgtac
     6481 tgtgattttg cttccggcac catcaatatt gccattatgg atggtaaggt ctggcgtctg
     6541 ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc gtcgtggctc aaattcacag
     6601 ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct tcaaagggta ttatggtgat
     6661 ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg acggtaccga aaaacgttat
     6721 ctgccggacg gcacactggt actgggaaat acggccgcag agggtattcg ttgctacggt
     6781 gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg cgtcttcccg ttatccgaaa
     6841 cactggctga ccgtgggtga tccggcgcgt gaattcacca tgacgcagtc cgcgccgctg
     6901 atggtgctgc cggatccgga tgagtttgtg gtggtgcagg tgaaataatc cgtgagcggg
     6961 agctaaatgc tcccgttttt cattcaatca ggagcgggta tggctacgaa agaagaaaac
     7021 ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg aaccggatgt ctccggtagt
     7081 gcggcagata ttgcgcagcg agtggcggaa ctggaggaag agcttggaga tgcgggggag
     7141 gtcgcagagc cggatacgta ctcaccatcg aaagatgatc cgaccgcgca taaaggcgag
     7201 tcagttcctg aacagccagg cattgttacc ggagacgaaa cggggcaggt tacggtggtg
     7261 gcactggcga cactgcatac aaaatgtaat ggttcagtga tatttgtttc accgggaacg
     7321 tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg caggtggtct ggcaaaaagg
     7381 cagtaacagg agatgtcgtg gacggtttcg ataatctgtt tgatgctgcg cttgccggtg
     7441 tcaatgaagt cattctccgt gatatgggga tcagcgccgt cattacgtcc ggagaacttg
     7501 aggggacaca cctgacaggg gtttttgatg atcccgaaag tatttctttt gttgccgggg
     7561 ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa aacggcagat atatcgcagt
     7621 tacgccgtca ggatacgtta actatcgggg atgattcttt ctttgtggat cgtattactc
     7681 cggatgacgg gggatgctgt tatatccggt taaggcgggg cagtcctgcc ccacagaata
     7741 atgccagaat gaggcgatat gatgaaggg
//
LOCUS       DBKELA010000151         7737 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.151, whole genome shotgun sequence.
ACCESSION   DBKELA010000151 DBKELA010000000
VERSION     DBKELA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.151"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACUQR0_003713"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACUQR0_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HHV9110574.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="ACUQR0_003714"
     CDS             1104..2564
                     /locus_tag="ACUQR0_003714"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HHV9110575.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACUQR0_003715"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACUQR0_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HHV9110576.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACUQR0_003716"
                     /gene_synonym="ydfH"
     CDS             complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACUQR0_003716"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HHV9110577.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACUQR0_003717"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACUQR0_003717"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HHV9110578.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="ACUQR0_003718"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="ACUQR0_003718"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HHV9110579.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="ACUQR0_003719"
     CDS             complement(6729..7247)
                     /locus_tag="ACUQR0_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HHV9110580.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7737
                     /gene="ydeI"
                     /locus_tag="ACUQR0_003720"
     CDS             7523..>7737
                     /gene="ydeI"
                     /locus_tag="ACUQR0_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HHV9110581.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLIS"
BASE COUNT         1938 a         1811 c         1953 g         2035 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctagcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gctccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttc
//
LOCUS       DBKELA010000152         7721 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.152, whole genome shotgun sequence.
ACCESSION   DBKELA010000152 DBKELA010000000
VERSION     DBKELA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.152"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..830)
                     /locus_tag="ACUQR0_003721"
     CDS             complement(<1..830)
                     /locus_tag="ACUQR0_003721"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9110582.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="ACUQR0_003722"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="ACUQR0_003722"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HHV9110583.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /gene="tsuB"
                     /locus_tag="ACUQR0_003723"
     CDS             complement(2479..2706)
                     /gene="tsuB"
                     /locus_tag="ACUQR0_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HHV9110584.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="tsuA"
                     /locus_tag="ACUQR0_003724"
     CDS             complement(2720..3778)
                     /gene="tsuA"
                     /locus_tag="ACUQR0_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HHV9110585.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="ACUQR0_003725"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="ACUQR0_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HHV9110586.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="ACUQR0_003726"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="ACUQR0_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HHV9110587.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="ACUQR0_003727"
     CDS             complement(5528..6511)
                     /locus_tag="ACUQR0_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHV9110588.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="ACUQR0_003728"
     CDS             complement(6557..7381)
                     /locus_tag="ACUQR0_003728"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHV9110589.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="ACUQR0_003729"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="ACUQR0_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HHV9110590.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcactc a
//
LOCUS       DBKELA010000153         7550 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.153, whole genome shotgun sequence.
ACCESSION   DBKELA010000153 DBKELA010000000
VERSION     DBKELA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.153"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="ACUQR0_003730"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="ACUQR0_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="ACUQR0_003731"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="ACUQR0_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HHV9110591.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3039
                     /gene="rbsB"
                     /locus_tag="ACUQR0_003732"
                     /pseudo
     CDS             2153..3039
                     /gene="rbsB"
                     /locus_tag="ACUQR0_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3165..4094
                     /gene="rbsK"
                     /locus_tag="ACUQR0_003733"
     CDS             3165..4094
                     /gene="rbsK"
                     /locus_tag="ACUQR0_003733"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HHV9110592.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4098..5090
                     /gene="rbsR"
                     /locus_tag="ACUQR0_003734"
     CDS             4098..5090
                     /gene="rbsR"
                     /locus_tag="ACUQR0_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HHV9110593.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5056..6483)
                     /gene="mdtD"
                     /locus_tag="ACUQR0_003735"
     CDS             complement(5056..6483)
                     /gene="mdtD"
                     /locus_tag="ACUQR0_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HHV9110594.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6506..7198)
                     /locus_tag="ACUQR0_003736"
     CDS             complement(6506..7198)
                     /locus_tag="ACUQR0_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HHV9110595.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1921 a         1826 c         1986 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcag attttgatcg caccaaaggt ttgaacgtaa tgcagaacct gttgaccgct
     2761 catccggatg ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc
     2821 gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat
     2881 ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag
     2941 attggcgcga aaggcgttga aaccgcagat aaagtgctga aaggcgagaa agttcaggct
     3001 aagtatctgg ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc
     3061 tgatggtgac ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca
     3121 cgcgccaccc taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca
     3181 gcctcgttgt tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta
     3241 ctccaggcga aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga
     3301 atcaggctgt ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg
     3361 atgacagcat tggcgagagc gttcgccagc agctcgccac tgataacatc gatattactc
     3421 cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag
     3481 gtgagaatgt catcggtatt catgccggcg ctaatgctgc tctttcccca tcgctggtgg
     3541 aagcgcaacg tgagcgtatt gccaacgcgt cagcgttatt aatgcagctg gaatcaccac
     3601 tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta
     3661 acccggctcc ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc
     3721 caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag
     3781 cgaaggcggc gcaggtactg catgaaaagg gtatccgtac tgtactgatt actttaggaa
     3841 gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc
     3901 aggctgtcga taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc
     3961 tggaagaaaa accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg
     4021 taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag
     4081 acaggcagag gtgacgcttg gctacaatga aagatgttgc ccgtttggcg ggcgtttcta
     4141 cctcaacagt ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca
     4201 aagttgaagc ggcgattaaa gaactcaatt acgcgccatc agctctggcg cgtagcctca
     4261 aactcaatca aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt
     4321 cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt
     4381 gcaataccga aggcgatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac
     4441 gcgttgatgg cttactgtta ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc
     4501 aacgttatcc gacagtgcct accgtgatga tggactgggc tccgttcgat ggcgacagcg
     4561 atcttattca ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata
     4621 aaggtcatac ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc
     4681 ggttggaagg ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg
     4741 aagtcactgg tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgttatcac
     4801 atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc
     4861 aggcgttata tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg
     4921 atatcgaact ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg
     4981 aactggggga gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc
     5041 aacaacgatt acaacttact ccgattctga tggaacgcgg ttcggcatag atttacgctg
     5101 tcttttgatc aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc
     5161 aacagtaata atgcccatcg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc
     5221 ttccatccct tcataaatgc gaaggacggc cgcacttaca gcaacgccta aactaatcga
     5281 cagttgctgc gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc
     5341 ggcaagtgtg atggtattca tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa
     5401 caacggcaag atcagcatcc atatcgccat tgccggtgat tgcaaagaga actgagcgat
     5461 cattagccca ataatcaccg tgatccccac taatgtatgg cgatagccca gacgacgtaa
     5521 gacttgggta accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca
     5581 gccggcaata aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg
     5641 tacacagccg gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg
     5701 ggttttaaat aaatctaatg aaattaatgg gtttggcgta cgtcgcgcat ggagaatata
     5761 gagaagcagt aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat
     5821 cttttccccg aatagctcta ttccgcttga aaagagaaca aggctgaggc caaacagcaa
     5881 aaagccagtg atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc
     5941 gtaaagaagg cccgcaatac ctatggggat attaattaaa aatatccagt gccaggttgc
     6001 ccaggtgacc agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat
     6061 ggcgacaaaa ttcaataccg gaagaagttc attacgagga taagcacgca gtaaggccgg
     6121 ccgagcaaca ggcatcatca ttgcgtcgcc tatcccttga ataacccgga agacaaccag
     6181 ctgtggtagc gaattagaaa gtgcgtaggc cagagaaccc aacgtgaaca gactcacggc
     6241 aagggtaaaa atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag
     6301 catcgccacc gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt
     6361 aaggctatga gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat
     6421 gaagaaggcc atcgccgcga tccacggcaa acccgacata ctacgcttct ttttatcgct
     6481 cattcaatgt cctgttatcg ggttatcact tatcaggtga gcgtagcagc gcctgacaag
     6541 ctttaaatgc cgcgtcgcca tcgctttgga taatcgcatc gacaatcgcc tggtgcagat
     6601 ccagctttat cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga
     6661 atagcgaggc gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc
     6721 aggccatatc gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt
     6781 cggccattaa cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gctgccagta
     6841 ggcaggcttg cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa
     6901 agttctcttc tgttatccac caagtaagca attcctgatc aagaaaattc cagttcgatt
     6961 gtggcatgac ccgagtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg
     7021 ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct
     7081 caccgggcaa aatggtgccg ggttcatatt caccttttaa gatccgttgc gccagcttct
     7141 cagccagaac atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta
     7201 cttatcttcc tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt
     7261 tgcaaaaacg gcaaattgct tgttttatgg cacattaacg gggtattttg ctgaaaaaat
     7321 gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact ccctataata
     7381 cgccaccact gacacggaac aacggcaaat acgccgccgg gtcagcgggg ttctcctgag
     7441 aattccgaca gagaaagcaa aaataaatga ttgactctgt agcgggaaag cgtattatgc
     7501 acacccccgc gccgctgaga aaaagcgaag cgcactgctc tttaacaatt
//
LOCUS       DBKELA010000154         7184 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.154, whole genome shotgun sequence.
ACCESSION   DBKELA010000154 DBKELA010000000
VERSION     DBKELA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.154"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(371..1198)
                     /locus_tag="ACUQR0_003737"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACUQR0_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACUQR0_003738"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACUQR0_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HHV9110596.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2480..4015
                     /gene="lsrA"
                     /locus_tag="ACUQR0_003739"
     CDS             2480..4015
                     /gene="lsrA"
                     /locus_tag="ACUQR0_003739"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HHV9110597.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4009..5037
                     /gene="lsrC"
                     /locus_tag="ACUQR0_003740"
     CDS             4009..5037
                     /gene="lsrC"
                     /locus_tag="ACUQR0_003740"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HHV9110598.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5037..6029
                     /gene="lsrD"
                     /locus_tag="ACUQR0_003741"
     CDS             5037..6029
                     /gene="lsrD"
                     /locus_tag="ACUQR0_003741"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HHV9110599.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6041..7064
                     /gene="lsrB"
                     /locus_tag="ACUQR0_003742"
                     /pseudo
     CDS             6041..7064
                     /gene="lsrB"
                     /locus_tag="ACUQR0_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7091..>7184
                     /locus_tag="ACUQR0_003743"
     CDS             7091..>7184
                     /locus_tag="ACUQR0_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HHV9110600.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1674 a         1811 c         1878 g         1821 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttaatgaac aaattaaaag tagaaataca tttgttcaaa actcacctgc
     2461 aaaactgaac gggggaaata tgcaaacgag tgatacccgc gcgttaccgc tactttgcgc
     2521 ccgctcggtt tataaacagt attcaggggt caatgtcctg aaaggcatcg attttacgtt
     2581 gcatcagggg gaggtccacg ccctgctcgg cggcaatggt gccggtaaat cgacgttaat
     2641 gaagattatt gccggtatta cccctgctga tagcggtacg ctggagattg ggggcaacaa
     2701 ctacgccaga ttaacgccag ttcatgctca tcagctgggc atttatctcg ttccccagga
     2761 accgctgctt ttcccaagcc tgtcgataaa agaaaacatc ctgtttgggc tggcaaaaaa
     2821 acagctctcc atgcagaaaa tgaagaactt gctggcggcg ctgggctgcc agtttgatct
     2881 gcatagtctg gcaggatcgc tggatgtcgc cgatcgccaa atggtggaaa tcctccgcgg
     2941 gctgatgcgc gactcgcgga ttctgatcct cgatgaacct accgcctcgc ttacccctgc
     3001 ggaaaccgaa cgcttgttta gtcgcttgca agagctgctt gctactggcg tgggtattgt
     3061 ttttatctcg cataagctgc cggaaattcg ccagattgcc gatcgaatta gcgtgatgcg
     3121 cgacggaacc atcgacttaa gcggcaaaac cagcgaactg tctaccgacg acattattca
     3181 ggccatcacc ccagcggtac gggaaaaatc gctctctgcc agccaaaaat tatggctgga
     3241 gttacctggt aaccgcccac aacatgccgt cggaacgccg gtgctgacac tggaaaatct
     3301 gactggcgaa ggcttcagga atgtcagcct gacgctcaat gccggagaaa ttctgggcct
     3361 ggctgggctg gtgggagccg gacgcacaga actggccgag acgctctatg gtctgcgtac
     3421 tttgcgtggc ggacgcatta tgctgaatgg taaagagatc aataaattat ccactggaga
     3481 acgtttactg cgcggtctgg tttatctgcc ggaagatcgc cagtcatccg gactgaatct
     3541 cgatgcttcg ctggcctgga acgtctgcgc ccttactcat aaccttcgtg gattctgggc
     3601 gaaaaccgcg aaagataatg ccactctgga acgttatcgt cgggcgctga atattaaatt
     3661 taaccaaccg gaacaagctg cacggacatt atccggtagc aaccagcaaa aaatcctcat
     3721 tgccaaatgc ctggaagctt cgccgcaagc attgattgtc gatgagccga cgcgcggcgt
     3781 ggatgtctcg gcccgtaatg atatctacca gctgttgcgc agcatcgccg cacagaatgt
     3841 ggctgtgctg tttatctcct ctgatatgga agagatcgaa ctgatggcag atcgtgtgta
     3901 tgtgatgcat cagggcgaaa ttacccactc tgcactgacc gggcgcgata ttaatgtcga
     3961 gactattatg cgcgttgcct tcggcgatag tcagcgtcag gaggcatcat gctgaagttt
     4021 attcagaaca accgtgaaat cacggcattg ctggcggtgg tgctgctgtt tgtattaccc
     4081 ggttttctcg accgccagta tttaagtgtg caaacgctga ccatggttta tagcagcgcg
     4141 caaatcctga tcctgctggc aatgggcgcg acgctggtaa tgcttacgcg caatattgat
     4201 gtttcagtgg gttcgattac cggaatgtgc gcggtgctgt tggggatgtt actgaacgca
     4261 ggatattcac tacctgttgc ctgtgtcgcg actttactgc ttggtttgct cgcgggattt
     4321 ttcaacggtg ccctggtcgc gtggctaaag atccctgcca ttgttgccac ccttggcacg
     4381 ttagggttgt acagaggcat catgttgctg tggactggcg gtaaatggat tgaagggtta
     4441 cccgccgaac tgaaacagct ctccgccccg ctgctgcttg gcatttcagc aattggttgg
     4501 ttgacgataa ttctggtggc atttatggcc tggctgctgg cgaagacggc gtttggacgc
     4561 agtttttatg ccacgggcga taatttacag ggcgctcgtc aactgggcgt tcgtacggaa
     4621 gccattcgca ttgtggcatt ttcgttgaac ggctgcatgg cggcactggc gggaattgtg
     4681 tttgcttcgc agattggttt tatcctcaac cagaccggta ccgggctgga gatgaaagca
     4741 attgcagcct gcgtgctggg cggcattagt ttgctcggtg gttccggtgc gttcatcggt
     4801 gcggtactcg gcgcatggtt cctgacgcag atcgatagcg tactggtgct gttgcgcatt
     4861 ccggcatggt ggaatgattt tatcgcgggt ctggttctgc tggcggtgct ggtgtttgat
     4921 ggacgcctgc gttgtgctct ggaacgtaat ctacggcggc aaaaatatgc ccgctttatg
     4981 acgccaccgc catccgttaa acccgcttcg tcaggtaaaa aacgggaggc cgcataatgc
     5041 gtattcgcta cggttgggaa ctggctcttg ccgcactgct cgttattgag attgtcgcat
     5101 ttggtgcaat taacccgcga atgttagatc tcaatatgtt gctgttcagc accagtgact
     5161 ttatctgcat tggcattgtc gctctaccgc tgacgatggt gattgtcagt ggcgggatcg
     5221 atatttcgtt tggttcgacc atcggcctct gcgccattgc attgggcgta ctgtttcaaa
     5281 gtggtgtgcc gatgccgctg gcgatactcc tgaccttact gctcggcgca ttgtgcgggc
     5341 tgatcaacgc cggattaatt atctatacca aagttaaccc gctggtgatt acgcttggca
     5401 cgctgtatct gtttgccgga agcgctctgc tgctttccgg tatggccgga gcgacggggt
     5461 acgaaggtat tggtggattc ccgatggcgt ttacagattt cgctaacctg gatgtgctgg
     5521 gactccccgt tccgctgatt atcttcctga tatgtctcct cgttttctgg ctctggctgc
     5581 ataaaaccca tgccggacgt aatgtgtttt tgattgggca aagcccgcgc gtggcgcttt
     5641 atagcgcgat tccagttaac cgcaccttat gtgcgctcta tgccatgacg gggctggcgt
     5701 ctgcggtcgc cgctgtgctg ctggtatcgt attttggttc agcacgttcc gatctcggtg
     5761 cgtcgtttct gatgcccgcc atcaccgccg tggtgcttgg cggggccaat atttatggtg
     5821 gttccggttc cattatcggc accgccattg cggttttatt agtgggatat ttgcagcaag
     5881 gtttgcaaat ggcaggagtg ccaaatcagg tgtccagcgc cctttccggt gcgctgctta
     5941 tcgtcgttgt cgtaggtcgt tccgttagcc tgcatcgcca gcaaattaaa gagtggctgg
     6001 cgcgtcgggc caataaccca ttgccataaa ggatatcttc atgacacttc atcgctttaa
     6061 gaaaatcgcc ttacttagcg ctcttggcat tgccgcaatc tctatgaatg tgcaggccgc
     6121 agagcgtatt gcatttattc ccaaactggt tggcgtggga ttttttacca gcggtggcaa
     6181 cggcgcacaa caagcgggta aagagctgag cgttgatgtg acctacgacg ggccgacaga
     6241 acccagtgtt tctggtcagg tacagttgat taataacttc gtcaatcaag gttataacgc
     6301 cattattcgt ttctgcggtt tcgcctgatg gcttgtgtcc ggcactgaaa cgcgccatgc
     6361 aacgtggtgt gagagtgctg acctgggact ctgatactaa accggagtgc cgctcttact
     6421 acattaatca gggaacgccc gcccagttgg gaggtatgtt ggtggatatg gcggcgcgtc
     6481 aggtgaataa agacaaagcc aaagtcgcgt ttttctactc aagccccacc gttacggacc
     6541 aaaaccagtg ggtgaaagaa gcgaaagcga aaatcgccaa agagcatcca ggctgggaaa
     6601 ttgtcactac gcagtttggc tataacgatg ccactaaatc gttacaaacc gcagaaggaa
     6661 tattaaaagc gtatagcgat ctcgacgcca ttatcgcccc cgatgccaac gccctgcccg
     6721 ctgccgcaca agccgcagaa aacctgaaaa atgacaaagt agcgattgtc ggattcagta
     6781 cgccaaatgt gatgcgcccg tatgtagagc gcggcacggt gaaagaattt ggactgtggg
     6841 atgtggttca gcaaggcaaa atttcagtgt atgtcgcgga tgcattattg aaaaaaggat
     6901 caatgaaaac gggcgacaag ctggatatcc agggcgtagg tcaggttgaa gtctcgccaa
     6961 acagcgttca gggctatgac tacgaagcgg atggtaatgg catcgtactg ttaccggagc
     7021 gcgtgatatt caacaaagag aatatcggca aatacgattt ctgatgtgca ttacttaacc
     7081 ggagtaagtt atggcagatt tagacgatat taaagatggt aaagattttc gtaccgatca
     7141 accgcaacaa aatatccctt ttaccctgaa aggttgcggt gcgc
//
LOCUS       DBKELA010000155         6790 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.155, whole genome shotgun sequence.
ACCESSION   DBKELA010000155 DBKELA010000000
VERSION     DBKELA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.155"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACUQR0_003744"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACUQR0_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HHV9110601.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="ACUQR0_003745"
     CDS             2089..2238
                     /locus_tag="ACUQR0_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHV9110602.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="ACUQR0_003746"
     CDS             complement(3005..3445)
                     /locus_tag="ACUQR0_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HHV9110603.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="ACUQR0_003747"
     CDS             3663..4052
                     /locus_tag="ACUQR0_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110604.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="ACUQR0_003748"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="ACUQR0_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061586541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="ACUQR0_003749"
     CDS             complement(4607..5443)
                     /locus_tag="ACUQR0_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HHV9110605.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..5718)
                     /locus_tag="ACUQR0_003750"
                     /pseudo
     CDS             complement(5443..5718)
                     /locus_tag="ACUQR0_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5793..6266)
                     /gene="dfrA14"
                     /locus_tag="ACUQR0_003751"
     CDS             complement(5793..6266)
                     /gene="dfrA14"
                     /locus_tag="ACUQR0_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HHV9110606.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6287..6790)
                     /locus_tag="ACUQR0_003752"
                     /pseudo
     CDS             complement(6287..6790)
                     /locus_tag="ACUQR0_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1585 a         1759 c         1771 g         1675 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgt cgctatcaat cgcggaaaaa ggcgtagtgc gcgaagcgaa
     5761 cgaagctttt tgccgtcgat tgcatagctt tgttaaccct ttttccaaat ttgatagcaa
     5821 tagttaatgt ttgaagtaaa gtgttgctca aaaacaactt cgaaggtatt tggaatactc
     5881 gggaagaaaa catccccctc tggctcgatg tcgatcgtcg ataagtggag cgtagaggcc
     5941 atgggtaatg tttctcggta aatttctccg ccaccagaca ctataacgtg accggtgaat
     6001 tcagctagcc tgtccatggc ctcttcgatt gactgaaata caactacatt gtcatcattt
     6061 gatgtccaac ctgagcgggt aacgaccgcg tatttcctat tggggagtgc gcccatagat
     6121 tcaaacgtct tgcgacccac cagaagccac tgattgtagg tcaatgcttt aaaaagtagc
     6181 tgctcccctt tcgcggacca gggtatgtct ggaccgcaac caatcacgcc gtttttcgct
     6241 ttcgcagcta tcaatgatac tttcaaggtt ctcatcctgg gttaacggtg cgctcttggt
     6301 cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc ggcgtactct
     6361 tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca tcaacggcgc
     6421 gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat aggctgtgaa
     6481 cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct ccagacagat
     6541 cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc tgttcctcct
     6601 gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg aggcggtcac
     6661 gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag gcatgcccgt
     6721 caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac cagtcagaat
     6781 gcgattcacc
//
LOCUS       DBKELA010000156         6670 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.156, whole genome shotgun sequence.
ACCESSION   DBKELA010000156 DBKELA010000000
VERSION     DBKELA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.156"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(152..2155)
                     /locus_tag="ACUQR0_003753"
     CDS             complement(152..2155)
                     /locus_tag="ACUQR0_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HHV9110607.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2152..3288)
                     /locus_tag="ACUQR0_003754"
     CDS             complement(2152..3288)
                     /locus_tag="ACUQR0_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HHV9110608.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3281..5560)
                     /locus_tag="ACUQR0_003755"
     CDS             complement(3281..5560)
                     /locus_tag="ACUQR0_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HHV9110609.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5571..6659)
                     /locus_tag="ACUQR0_003756"
     CDS             complement(5571..6659)
                     /locus_tag="ACUQR0_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHV9110610.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1531 a         1834 c         1787 g         1518 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat taccaactgg
       61 tgactctcac caggggctat ataggatatg cctaataccg tggcgtgagc agtccggaac
      121 tggagtagaa ctcttagtaa aaagcactat ttcatccttg ttgctgaagc atggggaata
      181 attgttcgca aagcaaaaca ccgttattca ttgcttctac ccgtgcctcg ctttctgtat
      241 tacgaaattg tgccaacaca tgtgccagcc gataaaaacc caccgcggtg aggtcattcg
      301 ccagcaactc tgcctgatta atagcgctct gttcctgata gcgccagccg ttatggagca
      361 gttgaataag taacgcctgg cagcgcatca gcaactgatg agcagtagac ggcacaggca
      421 gaacgctggc agaagatagt gataccacca caggcgcagt ttctgcgtcc agcgcccagg
      481 cacgagtctt tgtcatcatt acctgtggtt ccagtgtcaa ttgcccatca acaaaactga
      541 caaagccaga aaccagacac acggggtcgt ctgtttgttg caaaagcgcc gccatgcgtt
      601 caatggcata aggcgcgctg gctgaggctg gcagtgataa cgtcagcaga ttatcttcac
      661 cttcgccgct gattacctgc gcatccagcg tctggcggct gctatcccaa ccgagcgaaa
      721 tacactcagc gaccggcaga ataaataagt tatcgacctg attaagaggc cgtatgcagg
      781 cggggggacg ctggcgtaaa tattcccgca aagccacaat gcccggctgg cgtaacggcg
      841 cgctcaacat ttgccaggca tcaggcgaca gcggtacaac gctgcttaag cggttgcggg
      901 tagctaacag cagctcgcca tcggcactgc gttttgctgc ttgtgaaaca atttgcccac
      961 ccgccagtgc gccagcctga aaactaaaca gccgacgcgt agctgccggt gagtcttcct
     1021 gttcacttcg cggccaactg cgcgaaaggt gcaaaatact gccggtgtcg ggatcggtaa
     1081 accagatgcg taaaccataa tgctcaatat cctgccagca acgcatacct aaagacacca
     1141 gccgcagatg atcaagcttt gcttctccgg caatgccaga gccaacgacc gtgcgccacg
     1201 gcacaggagg aacttcacca acactgtcgc tccgggccat ctcttgtgcg caatttaatc
     1261 gactgtttaa tgccgcaagc tgacgtaagc attctccggc atgatagtgg ctggcgcggg
     1321 cgtggaaggc atcaacgctg gcgcgcagtt gccgtagcga ttcactcacc catcgccagc
     1381 tgcagcgttc cgctgcttgc tgcgcgcggc tgaaagcggc ctcgtagtga ataagcggct
     1441 ggctgatgcc gctcagccat aatgcctggc ttagttgttg aacatattga cgacacgttt
     1501 tgccttcttc gctggcaaac ggatcgtcag atgatgtgac gtgttcgctg cgcatctgcc
     1561 agattaaatg agtcaattcc gcttgctgcg cttttgcctg gacaaaggcc tgtactgcca
     1621 gtacgacatg ttcgcaaagt gtgccttcaa tacaatcaca acgggcgaaa cgaatactgc
     1681 tgcgggaata aaaacgcaca tcgctcatcg gtaagcgggc agagggaatt tcacccggcg
     1741 cacagaacaa ctcaatggtg atgcttttag cgaccagcgc ctgtgcgcgt ttgcgggtgg
     1801 catcgggaag ggtagccagt tcttccagcc agattgccgg atcccattcc tcttcttttc
     1861 ccgtaggctg ggcggtagca caaagtcgtt gataacttaa caccagcatc acgcgatgac
     1921 ggcacatgcc gctggcccca taggtgcact gagcctcttt cagtgcctgg ccgttcgcca
     1981 gctgggtacg gacaccgtca ctgaaggtgg cgattaaagc gctgttctca tggctgattt
     2041 ccgggacgtt gccattttcc agttccttaa ggctgcgctt aacaaaaccg gcattgctta
     2101 acgccgtcag tgcctgcggt gtcagttcta ataattccgg acgtagtgaa ttcatgactg
     2161 aagattctcc gcaagccatg atgccagctc gcccggtgtc atggcggcta tttgtgcgcc
     2221 gacattaatc agcgcctggg ccgtatcgcg gtcatagcaa ggtgttgctg tgctatcgag
     2281 cgctgccagt cccagcactt tgatgccgct ctggacacac tttttcactt ggtgcgtcag
     2341 caatgatgat gaaccccctt cataaaaatc gctcacgagg ataatgacgc tttttgcagg
     2401 ttgttcaata agttgccgac catactccat ggcactggcg atattggttc cgccgcctaa
     2461 ttgcactttc attaataact ctaccggatc ggcaacgtct gccgtgagat caacgacgct
     2521 tgtgtcaaac gccaccagat gggtacgaat gccgggtaac tgccataaac aggccgccat
     2581 caccgcagag tggatcaccg aatcgaccat cgatccgctt tgatcaacca gtaagaccag
     2641 ttgccattgc tcgctttggc gtttaatgcg gctgttaaag cgggggaatt cgatatacaa
     2701 cttgccgtgt tgcgggtgcc agtgttgcag gttggcgcgc agagtacttt tgaaatcaaa
     2761 gtttcgcgcc agtggaataa atgagcggcg acgacgatcg cggacaccag aaaaagcctg
     2821 acgaacttcc tttgccagtc gcgccataat ttcttccaca acctggcgca caatcctgcg
     2881 ggcggcagcc agtacttcgg ggttcatcag atgtttggtg tgcaaaacgg cgcgtagcag
     2941 gctttcggaa ggctgcatac gttccagcac gtcgagattt gtcaccacat cttcaatgcc
     3001 gtagcgcagt acggcatcgc tttccagccg ctcaatcacc tgttgcggaa acagcgtgtg
     3061 aatactgttg atccactcag gagtggtgag atttgagcca cctaatccac tagagcgttc
     3121 accacgctgg agccgttcag gatcgcgccc atacagccac tccagcgcgt gatctatctg
     3181 ccgggcgttg tcatccagcc cacaaagcgt cgtttctgcc gcttcgccaa gaattaatcg
     3241 ccagcgttgt agctcacggg tggtcagaag atcgttcagt tcagacatgg aaaactcccc
     3301 agtgttgcag cgatgccagt gcctgctgtt cgagttgttg atgatgtgca atggcttgcg
     3361 gtggacaatg caacggcatt tgcagtgccg aaacgggaag ttgcgccagt tgataatgct
     3421 caagcacctg atgcgccagc gtcccgcgtt ctcgtggcgg tagccaggcc attgccgcgc
     3481 gtaaatcggg cagggcattg gtaaaatcgg catcacttag ttgatttaaa tgactgctga
     3541 aaccggcgat aaatgccggc tgacaggcca gttgatgacg ggctaatgcc agcaaaccgt
     3601 gcagcgcctc gccggattgt gccggggata actgcgccag catcgtcagc gccgcttcgg
     3661 cactagcgtt gggatgttcc agacgcatca gcgcaccaag agtcgcgccg cgatccagtg
     3721 ccaggacatg aatcgcctga ctgtgtcgct ccagcaaggc taccgccgca gaaagcgaaa
     3781 cgccggggag ttgcacaccg ctatgtagat cgcgcaggat atgacacaac gcttgccagc
     3841 tatgcaagtg agcgtgaaac tccttttcat ccggtctgcc gttggattca cacagccaca
     3901 gcgtgcgatc gatagccgcg cataacgtcg tttgtaatat ctgcgcgcct tgcataccgc
     3961 taatttcatc cagccgccat aaggcatata gcacttccag cgcctggccc atttcggcaa
     4021 attgattttc ctgggcgatt aataatgtga gttgctccag taattgttga ctgaaggacg
     4081 ccagacccgc tgacgccgct tggctaagac atgtggtaat gctgccgatt ccacccgcgt
     4141 ccagcatatc ggcttctaat ttatggcgtg cggcttcctg gagtgtggca ccaaagcagg
     4201 cggcctcaat caatgccgca tgctggctaa gcgggcgggt taatttccag cgttcttcac
     4261 cgttgcctgc aagtgtcagt gtgcttccct gctggcgtac aatccctggg atctccagta
     4321 ttgccagccg atgtaacacc tgactttgcg ctagtccatt ggggttaaag cgatttaagg
     4381 taagctccgc cggaagagag attgcggtac gttccagttc gcaagtgaca tcttttggca
     4441 gaggcggttg tggcgtagaa ggggcaagtt taccgaatcc gtcacccgct aatgtgtcta
     4501 tcaacgttag cagaatcgga tcggtatcgg gatgaataac gccgcgatag ctccacggca
     4561 acggcgcatt cagggcttct ttaatcagcg atcctgctat ggcatccagc cagtcagtgc
     4621 gtaacggtag tgtatgaccg cgcaactgtg ccagtgccat cgcatgcaga tgagcggcag
     4681 ccatatccgc ggtcgaagca ggcaacttgt gctggcgcaa acgggtgaga accgttttta
     4741 gcagttgttc tcccgcctgc tgtaagcccc actgccagca ccagttttgc cagaccgggg
     4801 caggcattcc tgaaaggtat cctgccagca catcaaggcg cttttcactg tagggggtga
     4861 gataacaacc tgtaacggca tctgccagcg acggcaattc tggcgtgtta attacctgcg
     4921 gacattcgcg ccacattttt gccagtgccg gagcgtgcca gccaccgcag acaactaaca
     4981 cgtcgccatt attttgctgc atcgcccatc caatccagcg ggccataaag gcttcgcgct
     5041 gacgatttag agcatcgccg ggggagtcgc cccgcagttg ggcaaaatag tgcgccagtg
     5101 cagagggtaa tgcagtttgc tggctttcat cttcgaacaa gtgatcccac agggtgtcgc
     5161 tgttatccat gcgggtggca cgcagcagca aggtctggct ttcctcttgc gtatcaggcg
     5221 aatcgtcctc ttcttcactc tgcgcccagc aaggcaaatc gatgaagtaa gtttgtgcct
     5281 gaatacgacg atcggcttgt agcgcctgcc actccggcga aaattcagca aatggcgtcc
     5341 aggcaccacg cccgggggct gcaccgtcct gatactggca ataactgtaa atagctaccg
     5401 gaagctggtg ggctaaaaac agttcgtcta cccggtcatt aaaatcagca gggccttcaa
     5461 tcaacacgta tcgtggccgc tggctttcga ttaacgattt caccagccgg gcgcaggccg
     5521 gactatgatg ccggatgccg acaattaacg gctcgctcat gcatctttcc tcacggcagg
     5581 cggtggcggg cttgataata agcctgccag tgagcttctt tatgtgtggc gacacgctgt
     5641 tcgaagtaac ggcgcagacg agcgcgatct tcttcgttat ctttgacgat ggttccggca
     5701 atgcagtcca ccaggtcggc tggctcacct gcgcgattcg ctaaaaacca ggcgcggacg
     5761 cctacggcgt gggccacgtt cacggcttca gcggtggaca taatcgctgt tagtgtatcc
     5821 attgacgttt tcttttcgcc gttggcacgc agatcgcgga aggtgcggac cagtaactcc
     5881 agtaccgcgt caggcacttt atgcggaata ccgctatggg ccagcaaacg cgccgaagca
     5941 ctggcgacaa gctccagctc ctgggcgaaa tccataatcg gaaagacagt ttcaaaatcg
     6001 aagcgacgct ttagcgcagc actcatttca ttgacgccgc gatcgcgggt attggcagtg
     6061 gcaataatat taaagccttc tcgcgcatag agctgactgg cttcaccagt gagttccggc
     6121 accgtcatca ccctgtcaga aagcatacct aacagacagt cttgcacttc cagcggtgta
     6181 cgcgttatct cttcaaaacg gacgattttg ccgtcgcgca ttcccaggta aagcggggca
     6241 gggaccagcg cttccgttga tggaccatga ttgataagca gggcgtagtt ccagccgtac
     6301 ttgatttgat cttcggtagt agatgcgccg ccctggatgg ttaacccggc atcgccgcta
     6361 atagaggttg ccagtaattc agacaggaga gattttgcgg tgccaggttc ccccaccaac
     6421 atcaaaccgc gtccggtggc aagagtgact aacatacgtt cgacagcgga gggactaatc
     6481 accactttac ggctaatatt ttgcgcgcta tcgccaagga taaaagcacg ggccgcaggc
     6541 aaacttaact gccaaccggg cgggcaaggt gcgttatcat tttgttttaa ttttgccagt
     6601 tcatcggcgt ataacacagc agcaggcgga cgctgcagat gattattctg cggtgacata
     6661 tattcatccc
//
LOCUS       DBKELA010000157         6619 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.157, whole genome shotgun sequence.
ACCESSION   DBKELA010000157 DBKELA010000000
VERSION     DBKELA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.157"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            64..2496
                     /gene="hsdR"
                     /locus_tag="ACUQR0_003757"
     CDS             64..2496
                     /gene="hsdR"
                     /locus_tag="ACUQR0_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HHV9110611.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2563..4032
                     /locus_tag="ACUQR0_003758"
     CDS             2563..4032
                     /locus_tag="ACUQR0_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HHV9110612.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4032..5693
                     /locus_tag="ACUQR0_003759"
     CDS             4032..5693
                     /locus_tag="ACUQR0_003759"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HHV9110613.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5914..6255
                     /gene="symE"
                     /locus_tag="ACUQR0_003760"
     CDS             5914..6255
                     /gene="symE"
                     /locus_tag="ACUQR0_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HHV9110614.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6302..>6619)
                     /locus_tag="ACUQR0_003761"
     CDS             complement(6302..>6619)
                     /locus_tag="ACUQR0_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HHV9110615.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1950 a         1581 c         1526 g         1562 t
ORIGIN      
        1 tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa gcgagaaccc
       61 cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac caaatgcgtt
      121 atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca ggaggtcaaa
      181 ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac ggtaaaatct
      241 gctgacatcg tgctgtacca caagccaggc attcccttag ccgtgattga agcaaaagcc
      301 aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg cctgctggac
      361 gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc caccgcagcc
      421 gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc cgctgaactc
      481 tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt gattactcag
      541 gactattacg acgatggcag cggcaaatcg cctcgttatt atcaacttca ggcaatcaac
      601 aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat ggcgaccgga
      661 acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc aaaaaataaa
      721 aaacgcattt tgttccttgc cgatcgcaac attctggtcg accaaaccaa aaataatgat
      781 ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga tcccgcttat
      841 gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca aaaagcgttt
      901 aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca tcgcggcagc
      961 gcatccgaag acagcgcctg gcgagagatc cttgattatt tcagttccgc cacccaaatt
     1021 ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta cttcggcgat
     1081 ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc tccttataaa
     1141 gttgtccgtg ttgatattga tgtcgacctg caaggctggc gcccaaccaa agggcaaact
     1201 gacttaaacg gcgaagtgat tgacgatcgt atctataacc agaaagattt cgatcgcacg
     1261 atggtgatcg acgaacgcac agaactggtt gccagaacca ttaccgacta cctcaagcgc
     1321 accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc agaacgtatg
     1381 cgccgcgccc tggttaatct caacccgcag caggtgaaaa agaacgacaa atacgtcatg
     1441 aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat caacccgaaa
     1501 aaaccgtacc cggttatcgc gaccacttca gagctgatga ccactggtgt ggatgctaaa
     1561 acctgcaaac tggtagtact agaccagaac atccagtcga tgaccaaatt caagcaaatt
     1621 atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac catcctcgac
     1681 tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc cgaaaaagtc
     1741 atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga gaaactcgaa
     1801 gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc tgcgccacca
     1861 taccaggtta ccgataccga tgatgtcggc ccatttccgg aagaagacga gaagaaaatc
     1921 cgcaagtttc acgtcaacgg tgtagcggtg ggcgttattg cacagcgcgt tcagtattac
     1981 gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa aacactgctc
     2041 aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga tcgcaaagaa
     2101 gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc agaagaagtc
     2161 ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca gccgccgtta
     2221 acccgcaaag agcgcgccga gaacgtgcac aagcggaact acttcacaaa atactctgaa
     2281 gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg cgtacaggag
     2341 atcgaaagta ttcaggtgct gaaacttaaa ccattcgaca gcatgggcac cttaccggag
     2401 attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag cgagctggaa
     2461 aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc gccttctgca
     2521 accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat cagctcagta
     2581 atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggacggcga tgcgcagcgt
     2641 ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga agaggcgctg
     2701 gaactggagc aggataacta tcaatatccg atcccacagc gttatttgtg gcgcagttgg
     2761 gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa tgatgatctg
     2821 tttccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg ctacgtggtg
     2881 aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact gcgccaggtg
     2941 atcaacaagc tgaacgaaat tgactttacc agcgccagcg aacgccatct gtttggtgat
     3001 atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga attctatact
     3061 ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg cgaatccatt
     3121 atggacccgg cctgcggtac gggcggtttt cttgcctgcg cgttcgatca tgtaaagaac
     3181 aaatacgtga agagcgtcgc cgatcatcag acactgcaac aacagatcca cggtgttgag
     3241 aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctgcacgg cattgaagta
     3301 ccggtacaaa ttcgtcacga caacaccctg aataaaccgc tttcctcctg ggatgagcaa
     3361 ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg tattgagaag
     3421 aactttccgg cagagatgca aacccgcgaa acggcggatc tgtttctgca actgattgtg
     3481 gaagtactgg cgaaaaacgg tcgtgcggcg gtggtattgc cggatggcac actatttggc
     3541 gaaggtgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct gcataccatc
     3601 gtgcgtttac cgaatggtgt gtttaatccc tataccggca ttaaaaccaa cctgttgttc
     3661 tttaccaaag gtcagccaac caaagagatt tggttctatg aacatccgta tccggcgggc
     3721 gtgaaaaact acagcaaaac caagccgatg aagtttgaag agtttcaggc ggagatcgac
     3781 tggtggggta acgaggccga tggttttgcc agccgcgtcg agaatgagca agcgtggaaa
     3841 gtcagcattg atgacgtgat tgcgcgcaac ttcaatctgg atattaaaaa cccacatcag
     3901 gcggaaaccg tcagccatga tccggacgaa ctgttagcgc agtatgcaaa acagcaggcg
     3961 gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct gtctggcaag
     4021 gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatggaaacc tggacctcgg
     4081 cgttgcaaac ccgttccacc gccgggcgcg gcagctccgg taagattgat ttgtatggta
     4141 ttaagaaatt gcgtgagctg attctggaac tggcggtgcg cggtaaactg gtgccgcagg
     4201 atccgaatga tgaaccggcg tcggacctgc tgaagcgtat tgcggcggaa aaagcagagc
     4261 tggtgaagca aggcaaaatt aaaaagcaaa aaccactgcc agaaattagt gaggaagaga
     4321 agccgtttga attgccggag ggatgggagt gggtgcacct ccctgatatt tattgctcaa
     4381 tttctgagtc atctagaaaa ataaaatcct ccgaaatact acctgaggga aaatatcctg
     4441 ttatcgagca aagccaagaa tttatttctg gttattgcaa caatgaatgt ctgttaataa
     4501 agttaaataa tccagttata gtttttggtg atcacacaag gaatattaaa tttattgatt
     4561 ttgattttgt tgtcggcgct gacggtgtga agattttatc acctatatta atttgtgagc
     4621 gtttcttttt ttggcagtta cgatcattta aattagatgt aagaggatac gcgagacatt
     4681 ttaaagtatt aaatagctgc ttatttgcat taccccccat tgcagaacaa gagcgaatag
     4741 ttgaaaaagt tagctctttg atgtccctct gcgaccaact agaacagcaa tccctgacca
     4801 gtctggacgc acatcagcaa ctggttgaaa ccctgttggg aacgcttaca gacagccaaa
     4861 acaccgcgga actggctgaa aactgggcgc gtattagcga gcatttcgac accctattta
     4921 ccaccgaagc cagcgtggat gcgttaaaac agaccattct gcaactggcc gtaatgggca
     4981 aacttgtgcc gcaggatccg aatgacgaac ccgcctctga actgctcaaa cgtattgcgc
     5041 aggaaaaagc tcaattggtg aaagaaggga aaataaaaaa acaaaaacca ctgccgccaa
     5101 ttagcgatga ggaaaaaccg tttgaacttc cggaagggtg ggaatggtgt cgtattggta
     5161 atatagttaa tattaaatct gaattagtat caccaaaaga ttatttaaat ttatatcaag
     5221 tagcacctga tattattgaa aagggcactg ggagagtgat atcaaagaga acagtgaaag
     5281 aatcaggagt caaagggcca aatagtcgct tttataaagg acaaattgtt tattctaaaa
     5341 tccgcccgtc attatccaaa gtttttttag cagagtataa tggtctttgc agtgctgata
     5401 tgtatccatt agactgttac attaatccaa attacttatt gaaatatatc ctttccattc
     5461 catttcttat gcaagtcaaa aaagcagaga acagaataaa aatgccaaaa cttaattctg
     5521 acagttttta caatattatt gttgcgattc ccccctataa tgaacaacaa gcaatatttg
     5581 ataagataaa ttctattgaa gcagtatgca atggtcttat ttcttatatt gggatttatc
     5641 ataaaaccca actgcatctt gcagacgcac tcactgacgc agcgataaac taattccctc
     5701 gccctcacaa aaccaataaa gcggctaaat agccgcttta ttcacatcac caaaatttat
     5761 atttcacgct aattttcatc ttattttcat taatttttag ctggactttc cctgtattta
     5821 ctgatgatgt atccaggtat ttagcacggt gcggatgtgc gccaacacac tcgcaccgct
     5881 aatcacaatc actatttata gagaatagta atcatgactg acacgcattc tattgcacaa
     5941 ccgttagaag cagaagtctc cccggcaaat aatcgtcaat taaccgtgag ttatgcgagt
     6001 cgttacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg gctggaagcc
     6061 gccggttttg ccaccggcac ggcggtagat gtcaaagtga tggaaggctg tattgtcctc
     6121 accgcccaac ccgccgccgc cgaagagagc gaactgatgc agtcactacg caaggtgtgt
     6181 aagctgtcgg cgcgtaaaca aaggcaggta caggaattta ttggggtaat tacaggtaaa
     6241 cagaaagtcg cctgatacat tgacccggta gcgccagtgc taccgggtat tattcgccgc
     6301 gttataccaa cgcgctctcc atctgttttt caaatgcctg acgaattaat cccataatgt
     6361 aaggaagttg tgcgaggtcg ctgaaaccaa tttctacatc gccattaccc cagcgcccaa
     6421 catttgtcac atctttggca atacctttcg gatcgaccag ttcatgaaac tgcatattaa
     6481 gcgacaatcg cagtcggctt ttttgcggca caacatcaac aaaattcgtt tccgctttaa
     6541 acgcaatata tagcttcaat acttcctgcg tgatccctgc gtccaggcgc ataacttcat
     6601 cgcgaagatg atcgaataa
//
LOCUS       DBKELA010000158         6413 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.158, whole genome shotgun sequence.
ACCESSION   DBKELA010000158 DBKELA010000000
VERSION     DBKELA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.158"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(34..1248)
                     /gene="rspA"
                     /locus_tag="ACUQR0_003762"
     CDS             complement(34..1248)
                     /gene="rspA"
                     /locus_tag="ACUQR0_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HHV9110616.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1454..1780)
                     /locus_tag="ACUQR0_003763"
     CDS             complement(1454..1780)
                     /locus_tag="ACUQR0_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HHV9110617.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1915..2256
                     /locus_tag="ACUQR0_003764"
     CDS             1915..2256
                     /locus_tag="ACUQR0_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HHV9110618.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2291..2851
                     /gene="speG"
                     /locus_tag="ACUQR0_003765"
                     /pseudo
     CDS             2291..2851
                     /gene="speG"
                     /locus_tag="ACUQR0_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2854..3564)
                     /locus_tag="ACUQR0_003766"
     CDS             complement(2854..3564)
                     /locus_tag="ACUQR0_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HHV9110619.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3672..3977
                     /locus_tag="ACUQR0_003767"
     CDS             3672..3977
                     /locus_tag="ACUQR0_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HHV9110620.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4176..5588
                     /locus_tag="ACUQR0_003768"
                     /pseudo
     CDS             4176..5588
                     /locus_tag="ACUQR0_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6292..>6413)
                     /locus_tag="ACUQR0_003769"
     CDS             complement(6292..>6413)
                     /locus_tag="ACUQR0_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110621.1"
                     /translation="ILGKVFVVAEAATTVKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1711 a         1630 c         1501 g         1571 t
ORIGIN      
        1 gttaaacccg tggcgattca attatcttac tccttacctg ttccacagcg tgccatcttc
       61 cagacgtgcg actggtagat aagcaggttc atagggatat ttcgccgcca gcttttcatc
      121 gaattcgata ccaagacccg gtttgtctcc cggatgcata tagccgttat cgaaagtcca
      181 gttgtgcggg aagacttcga gcatttgttc ggaataaccc atgtattcct ggacaccgaa
      241 attggggacc cacagatcaa agtgcagcgc cgcagccatg cagactggtg acaaatcgga
      301 aggaccgtgt gagccagtac gtacctgata cagcgaagca aaatcggcaa tccggcgcat
      361 accggtaatt ccgcctgcat gggtcagcgt ggtgcggata taatcgatga gttgctcttc
      421 aatcagttgt ttgcagtccc agatgctgtt gaagacttca cccactgcga tgggtgtgac
      481 ggtatgttgg cgaatgagac ggaagcattc ttggttttcc gctggcgtcg ggtcttccat
      541 ccagaacatg cgataatctt caatgctttt accaaagcgc gccgcttcaa taggcgttaa
      601 gcgatggtgc atgtcatgca gcaaatgttc attaaaacca aacttgttac gtaccgcgtc
      661 aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac cacagctgct cttccggcca
      721 ctgtcctttg gttgcgggtt cataagccag acctttacct ttcgacatgc cgtaggtggt
      781 tttcatacca gggattccgc actgcacgcg gatggctttg aatcccagct cctgatgacg
      841 ggcataatca tccagagctt catcaatact gtgaccggtg gtatggcaat aaaccatcac
      901 cccttcacga gacgcgccgc cgagtaactg gtaaagcggc atgttggcag ctttggcttt
      961 aatatcccac agcgccatat caaccgctga aatggccgac atcgtaaccg gaccgcgacg
     1021 ccagtaagca cctttataga aaaactgcca gatatcttcg atacggtgcg catcgcgacc
     1081 aataagccgc ggacaaagat gatcctgcaa ataagaggcc acggaaagct cacgtccatt
     1141 gagggtggca tccccaaggc ccgtaatacc gtcctcagtg gtgattttta atgtgacgaa
     1201 attacgcccc ggacaggtaa caaaaacttc agcctttacg atcttcatgt tcgattcctt
     1261 gcatcgcttg tcgtgatgca tgaaatctac gcaactgagc tactgccata caagtatgaa
     1321 gatcgaaaaa agccggagtg atcacaaaaa aaggcgtata tttgcgctgt gaatggttga
     1381 caaaagatga aatagaatac cttttgtcag ctgacacttc ctcgtatctt attgataaaa
     1441 tggatttatg ttcctacgtg cgcccccagc ccgcaacaat gatcaacatt ccgcaaagcg
     1501 caatcaacgc acccgtccag tcataaagac ttagtttcac gccatccaca acgcgcagcc
     1561 acatcaatgc cgtgcagaca taaacgccac cataagccgc gtaaacacgc ccactcgccg
     1621 caggatgcaa cgttaacaac cagacaaaca gcgccagtga aatccccgcc ggaagcaaca
     1681 gccagatact ggcgtttcgt tttaaccaca accagggcag aaagcatcca ataatttcac
     1741 acagcgcagt agcaaaaaat agtaacgttg ttttaatcat ctttgtcact tattgacatc
     1801 atgtatagtt atagggcgac ataatatcat caatataaac atcctcctgg tacgttttgc
     1861 gttcgcagtg gatggtgtag aatcaccttt aatcattcat acagggaatg aattatgaaa
     1921 atcactctca gcaaacgaat cggcctgctc gcttttctgc tgccttgcgc actggcattg
     1981 agcacaactg ttcatgccga aactaacaaa ctggtgattg agtctggcga cagtgcacaa
     2041 agccgccagc acgccgctat ggaaaaagag caatggaatg acacgcgcaa tctgcgccag
     2101 aaagtgaata aacgcactga aaaagagtgg gataaagccg acgccgcttt tgataaccgc
     2161 gataaatgtg agcaaagcgc caacatcaat gcctactggg agcccaatac tttgcgctgc
     2221 ctggaccgtc gaactggccg cgttattacc ccctaacctg ttattgattt aaggaatgta
     2281 aggacacgtt atgccaagcg cccacagtta agctacgccc gctggagcgt gaagatttac
     2341 gctatgtaca tcaactcgac aataacgcca gtgtgatgcg ttactgattt gaggaaccct
     2401 acgaagcctt tgttgaactc tctgatctgt atgataagcg catattcacg atcagagcga
     2461 acggcgcttt gtggtggaat gtgacggcga aaaagccggt ctggtggagc tggtggaaat
     2521 taaccatgtt catcgccggg cagaatttca gataattatc tccccggagt atcaggggaa
     2581 aggtctggca acccgtgccg ccaaattagc aatggactat ggcttcaccg ttctcaatct
     2641 ctataagctg tatctgatcg tcgataaaga gaatgaaaaa gcgattcaca tttaccgcaa
     2701 gcttggcttt acggttgaag gtgaattgat gcatgagttt tttattaatg gtcaatatcg
     2761 taatgccatt cgcatgtgta tattccagca tcagtatctg gcagagcaca aaacaccggg
     2821 tcagactctc ctgaagccga ccgcacaata gcattaataa taatcgatcg tatttttgat
     2881 ggtgtaaacc cgttcgacgg cgggttttac tccttcatca acaatgatca gctgacagtc
     2941 cactgggttg gcgtgactgt catattcaca gctctgtttt acattaccaa ctcgttgatt
     3001 atttagtaaa gtaaccgctg tgtaatctaa ttttttgatc ggatccgttg atggcgtggc
     3061 gctgacagat aatgttttgt cgttactttt cgtggtttta cccagcggat acccctgatc
     3121 atcatagcga tattccatct gcatttgttt gctgctggct ttaatcacga agccattatc
     3181 atcggtttcc caactcaccc cggcagaagg taattctgct agctggcatt ttccctgtaa
     3241 acgtactctc ttctccagcg tctcggcatc acggtaataa ttggcgtcca gtaccagagc
     3301 gaccacggta ttattttcca gatccagtaa ttcgagtgaa tcaaaacagc cttcttccga
     3361 caaagtccca gaaacacgtt tcgtcacttc accttgctca tccattaatg tctgagtgaa
     3421 atcttttacc ggaccacgca gcggatcaaa atcgaattca ttagagaaac tggccatctc
     3481 aggggtaaat gaaagtgtta cctctgtgcg gtcacatcct gtgaggaata tcgcgagtaa
     3541 gcatggtagt aatttgtatt tcacaacagt caccagagag tagagatgat tctcaatcat
     3601 agtagcaaat acagtacttt acacgttaaa tgctatgctt aaagaagtta tcttcgcgta
     3661 aggagcttat gatgaaactc tcaacctgct gtgccgcgct tctgctcgcc ctcgcctcac
     3721 ccgcggtact cgccgcgccg ggatcttgtg agcgcataca gagcgatata tcacagcgca
     3781 ttatcaataa tggcgtaccc gaaagcagct tcacgttaag tattgtaccc aatgaccagg
     3841 ttgatcagcc tgattcccag gtcgtcgggc attgcgctaa tgatacgcat aaaattctct
     3901 acaaccgcac caccagcggt aacgtctctg ctcccgcgca gtctacccag gatggcgcgc
     3961 ctgccgaacc gcagtaacac attatcgact gaacgccgga tatgacacat ccggcgattt
     4021 gaacatacaa cataatctca ccttattact catacccttc tattgatgtg gattaataat
     4081 tcttaaccca aaatgggtag actccctcta ttgttagcgc gctaaatatt caatatataa
     4141 acatttatat aacaataaag aacagggagt gagttatgtc caaaaatgac cgaatggtgg
     4201 gcatcagccg cagaacactc gttaaatcaa ccgcgatagg ttctctggcg ctggctgcag
     4261 gcggtttttc tttaccgttc accctgcgca gtgcagcggc agcggtacaa caggccagcg
     4321 aaaaagtgat ctggggttcc tgttccgtca actgtggtag ccgctgtgca cttcgtctac
     4381 atgttaaaga taatgaagtg acctgggtgg aaactgacaa taccggcagc gatgagtacg
     4441 gcaaccatca ggtacgcgcc tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc
     4501 ccgatcgctt gaatgaccca atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac
     4561 ggattagctg ggatgaagcc ctggatacta tcgccagtag cctgaaaaaa accgtcgaac
     4621 aatatggcaa tgaagctgta tatattcagt actcttcggg gattgttggc ggcaatatga
     4681 cccgctcttc gccatcagcc tcggcggtca aacgcctgat gaactgctac ggcggttcac
     4741 tcaatcagta tggctcctac agcaccgcgc aaatttcctg tgccatgccc tacacctacg
     4801 gcagtaatga tggcaacagc accacggata ttgaaaacag caagctggtc gtgatgtttg
     4861 gtaacaaccc ggcagaaacc cgcatgagcg gtggtggcat cacttatctt cttgaaaaag
     4921 cgcgcgagaa atcgaacgcc aaaatgattg ttatcgatcc gcgatatacc gatacggctg
     4981 ccggtcgtga agaggaatgg ctccctattc gcccgggcac cgatgccgcg ctggttgcgg
     5041 gtattgcctg ggtattgatt aacgaaaatc tcgttgatca accttttctc gataaatact
     5101 gcgtcggcta tgacgaaaaa accttacccg cagatgcacc caaaaatggt cactataaag
     5161 cctatattct tggtgaaggt gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa
     5221 ttaccggtat cccggtagac cgtatcatca aactggcgcg tgaaattggc acaacaaaac
     5281 ccgcttatat ctgctagggc tgggggccgc aacgccaggc aaacggcgag ctgactgcac
     5341 gcgctattgc tatgttacct attttgacgg gcaatgtcgg catcagcggc ggaaatagtg
     5401 gcgcgcgtga atcgacctat accattacga tagaacgcct accggtgctg gataatccgg
     5461 tcaaaacgtc aatctcctgc ttcagctgga cggatgctat cgatcatggc ccgcaaatga
     5521 cggcactccg cgacggtgtc cggggcaaag ataaactgga tgtgcccatt aagttcatct
     5581 ggaacttcaa ggacttgaag cggaccctta gtaggcctga ggtcctgcat tgagtcaaga
     5641 ccttgtgtca ttctttgtgc cagacttgtg tcattagacg ggatgtctag acggctggtc
     5701 agcccccccg gtggcctcgg tctggacttg aggtgggcct atggggggaa gtctgctggc
     5761 ttaatgtatt agatggtcat tcacattttt ctgtcagaaa ccatcggtcc cactgtgagt
     5821 ccaaacctaa ggacctagaa gtccacccac agccttagac agagtgtcga acacatccac
     5881 accgagtcga ccagagagga ccatgtagag acccacagag aggaacacga gtttatagac
     5941 cagagtcagg agggacttca ggtctgcgga agtcatagac ataaagcccc tatctgttat
     6001 gttatattat tatattaaca acaggtccag accattggtc cagaccatag ttagttaatc
     6061 acagtgatag cccaccacct gaagtcctgc tgatattcag tccagactta cctctgctca
     6121 tggtcttacc tccctcggtc tcctgacctt gggtatcgac tacagcttgc tgagcgtcat
     6181 ctggaggagc tacaggaggc gggaccgaag ccccaccttg agtcccacca ccacctgtca
     6241 ggcccttggc gatggacctg aagcctttac taatgctctt acccattggt cttacgcttc
     6301 ggtcttggtg aacttcaggc gtttgactac aggcaggacg gcagtagctg agccagtgcc
     6361 catcttgacg gtcgtagcag cttcagcgac aacgaacact ttaccgagga ttt
//
LOCUS       DBKELA010000159         6374 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.159, whole genome shotgun sequence.
ACCESSION   DBKELA010000159 DBKELA010000000
VERSION     DBKELA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.159"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..65
                     /locus_tag="ACUQR0_003770"
     CDS             <1..65
                     /locus_tag="ACUQR0_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110622.1"
                     /translation="QPEIDLIRPEFSTAWYPKND"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="ACUQR0_003771"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="ACUQR0_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
     gene            1476..1799
                     /gene="pgaD"
                     /locus_tag="ACUQR0_003772"
     CDS             1476..1799
                     /gene="pgaD"
                     /locus_tag="ACUQR0_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HHV9110623.1"
                     /translation="MFIFLFAMDLLTGYYWQSEARSRLQFYFLLAVANAVVLIVWALY
                     NKLRFQKQQHHAAYQYTPQEYAESLAIPDELYQQLQKSHRMSVHFTSQGQIKMVVSEK
                     ALVRA"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="ACUQR0_003773"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="ACUQR0_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HHV9110624.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="ACUQR0_003774"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="ACUQR0_003774"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HHV9110625.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="ACUQR0_003775"
     CDS             complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="ACUQR0_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HHV9110626.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5701..>6374)
                     /gene="efeU"
                     /locus_tag="ACUQR0_003776"
     CDS             complement(5701..>6374)
                     /gene="efeU"
                     /locus_tag="ACUQR0_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HHV9110627.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1533 a         1681 c         1533 g         1627 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gaatgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gccgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tagacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa acgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4741 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4801 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc
     4861 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4921 ggtataaagc tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc
     4981 tttttccaga cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc
     5041 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatacg
     5101 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     5161 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     5221 cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     5281 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     5341 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     5401 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5461 gctatggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5521 gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa
     5581 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5641 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5701 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5761 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc
     5821 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagaggaccg cgctcatatc
     5881 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5941 agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     6001 aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg cagtagcaag
     6061 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     6121 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     6181 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgttcca gttgcacttt
     6241 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     6301 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     6361 gttaatgaag atcc
//
LOCUS       DBKELA010000160         6335 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.160, whole genome shotgun sequence.
ACCESSION   DBKELA010000160 DBKELA010000000
VERSION     DBKELA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.160"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="ACUQR0_003777"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="ACUQR0_003777"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HHV9110628.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="ACUQR0_003778"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="ACUQR0_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HHV9110629.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="ACUQR0_003779"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="ACUQR0_003779"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HHV9110630.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /gene="actS"
                     /locus_tag="ACUQR0_003780"
     CDS             4130..4885
                     /gene="actS"
                     /locus_tag="ACUQR0_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HHV9110631.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="ACUQR0_003781"
     tRNA            4964..5037
                     /locus_tag="ACUQR0_003781"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..>6335
                     /locus_tag="ACUQR0_003782"
     CDS             5152..>6335
                     /locus_tag="ACUQR0_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HHV9110632.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DS"
BASE COUNT         1584 a         1586 c         1639 g         1526 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca atgaaatagc ccaactacag gtcaaagacg tcaggaccac tgaggcggga
     6301 accgtttata ttcatatcaa tgaagacgat agtag
//
LOCUS       DBKELA010000161         6328 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.161, whole genome shotgun sequence.
ACCESSION   DBKELA010000161 DBKELA010000000
VERSION     DBKELA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.161"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            244..1632
                     /gene="narU"
                     /locus_tag="ACUQR0_003783"
     CDS             244..1632
                     /gene="narU"
                     /locus_tag="ACUQR0_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HHV9110633.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1714..5454
                     /gene="narZ"
                     /locus_tag="ACUQR0_003784"
                     /pseudo
     CDS             1714..5454
                     /gene="narZ"
                     /locus_tag="ACUQR0_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5451..>6328
                     /gene="narH"
                     /locus_tag="ACUQR0_003785"
     CDS             5451..>6328
                     /gene="narH"
                     /locus_tag="ACUQR0_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HHV9110634.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1490 a         1683 c         1662 g         1493 t
ORIGIN      
        1 tgaagggttg aaacatgaca tacgttcaaa gattaacccc cgtttgaagg ttagcttgtt
       61 agttacattt agtaacacat atctggctac ttaatcccca ttctgccccc gcggttacgc
      121 tttcttatct attctttcaa tcagcacttc acccaattca tccttaattc taataactgg
      181 ctattgcgcc ttacctcctg ttttgtcttt tttcattacc aatgtgtgca tatgaggaac
      241 aatatggcac tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg gaaaccagaa
      301 aatccggcct tctgggaaaa taaaggaaaa catattgctc gaagaaacct ctggatatca
      361 gtcagttgtc tacttcttgc cttctgtgtc tggatgctat ttagcgcagt taccgttaat
      421 ctcaataaaa tcggttttaa ttttactacc gatcaactct ttttattaac cgcattaccc
      481 tccgtttctg gcgcattatt gcgtgttccc tactccttta tggtgcctat attcggtgga
      541 cgccgatggc cggtttttag tactgcaatc ctgattattc cttgcgtctg gctcggaatt
      601 gccgtgcaaa atccgaatac tccttttggg atatttatcg ttatcgcttt gctatgcggt
      661 tttgcaggtg caaactttgc ttcgagcatg ggcaatatca gtttcttctt tccaaaagcc
      721 aaacaaggga gcgctcttgg gattaatggc ggattaggaa acttaggtgt aagtgtaatg
      781 cagctggttg caccgctggt catttttgta cctgtatttg cctttctcgg cgtcaatggc
      841 gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat ttgggtaccg
      901 ctactggcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc cagttcacgc
      961 gcctcaattg ccgaccagct ccctgtctta caacgcctgc atctctggct gctgagcctg
     1021 ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc catgctggca
     1081 aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc atttattggt
     1141 gccatcgcgc ggtcggttgg tggtgctatt tccgataaat tcggcggcgt gcgggtgacg
     1201 ttgatcaact ttatttttat ggcgattttc agtgccctgc tgttccttac cttaccgggc
     1261 acaggctccg gtaatttcat cgccttttac gccgtattta tggggctgtt tctgaccgcg
     1321 ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca gataaccatt
     1381 tatcgggtga agatgaaagg cggtagtgat gagcaagctc agagagaagc cgtcaccgaa
     1441 acggcggcaa cgctgggctt tatctcagcc attggcgcag tgggcggctt ttttattccg
     1501 caggcatttg gcatgtcgct caatatgacc ggctctccgg tcggcgcgat gaaagtgttt
     1561 ttaatcttct acatcgtttg tgtgctgctg acctggctgg tttatggtcg gcggaagttc
     1621 agccaaaaat aagagatcga tgcctgatgt acaaccatgt cgcatccgac aaggcatcag
     1681 atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga tcgctttcgc
     1741 tacttcaaac aaaagggcga aacctttgcc gatggtcacg gacaggtgat gcatagcaac
     1801 cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaatcgt gcgttccacc
     1861 cacggcgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa tggtctggtg
     1921 acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgacctgcc caatcatgaa
     1981 cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tttacagcgc taaccgcctg
     2041 aaatacccgc tcattcgtaa acgactgatt gaactgtggc gcgaagccct caaacaacac
     2101 agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg cctgagctac
     2161 aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggcaggaact aaaccagctg
     2221 attgccgccg ctaacgtctg gaccatcaaa acctacggcc cggatcgcgt tgccggtttc
     2281 tcgccgatcc cggcgatgtc gatggtttct tacgccgccg gaacgcgtta tctgtcgctg
     2341 cttggcggca cctgttaaag tttctacgac tggtattgcg acctgccgcc cgcctcgccg
     2401 atgacctggg gcgagcaaac cgacgtaccg gaatctgccg actggtataa ctccagctac
     2461 atcatcgctt gggggtctaa cgtaccgcag acacgtacgc cggacgccca cttctttacc
     2521 gaagtacgct acaaaggcac taaaaccatc gccattaccc ctgactactc tgaagtggcc
     2581 aaattgtgcg accagtggct ggcaccgaaa caaggcactg atagcgccct ggcgatggca
     2641 atggggcatg tgattttaaa agagtttcat ctcgataatc ccagcgacta ctttatcaac
     2701 tactgccgcc gctacagcga catgccgatg ctggtaatgc tggagcctcg cgacgatggt
     2761 agctacgttc ccgggcgcat gatccgcgca tctgacctgg tggatggact gggcgaaagc
     2821 aacaatccgc agtggaaaac cgtagcagtt aataccgcag gtgaattggt agtgccgaac
     2881 ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc cattgccgcc
     2941 ggtacggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt tgcaggcgtg
     3001 gccttcccct actttggcgg cattgaaaat ccgcattttc gcagcgtaaa acacaatccg
     3061 gtgctggtgc gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa cacctgtccg
     3121 gtggtcagcg tttatgattt ggtactggcg aattacggcc tcgatcgcgg gctggaagat
     3181 gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg gggtgagcaa
     3241 attaccggcg taccgcgcca gtatattgaa accatcgccc gcgaatttgc cgataccgcc
     3301 cataaaacgc atgggcgctc aatgattatc ctcggcgcag gtgttaacca ctggtatcac
     3361 atggacatga actaccgcgg gatgatcaat atgctgatct tctgcggttg tgtcgggcaa
     3421 agcggtggcg gctgggcaca ctatgtcggc caggaaaaac tgcgcccaca aaccggctgg
     3481 ttgccactgg cctttgcgct cgactggaac cgaccaccgc gccagatgaa cagcacctcg
     3541 tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgctca ggagttactt
     3601 tcaccgctcg ccgatgccag taagtacagc ggtcatctga ttgatttcaa cgttcgcgcc
     3661 gaacgtatgg gctggctacc ttctgcgccg cagctggggc gtaacccgct cgggattaaa
     3721 gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc gctgaaatcg
     3781 ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc gcgtaatttg
     3841 tttgtctggc gctctaacct gcttggctcc tccggcaaag gccacgagta tatgcagaag
     3901 tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag cgacgggatc
     3961 aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga cctgctggtg
     4021 acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct gcccaccgcc
     4081 acctggtatg aaaaagacga tatgaacacc tcggatatgc atccatttat tcatccgctt
     4141 tctgcggcgg tcgatccggc ctgggaatca cgcagcgact gggaaatcta caaaggtatt
     4201 gccaaagcat tttcgcaagt gtgcgtgggc catcttggca aagaaaccga cgtggtatta
     4261 caaccactgc tgcatgactc tccggcagag ctctcacagc cgtgtgaagt gctcgactgg
     4321 cgcaaaggcg aatgcgatct gatcccgggt aaaaccgcgc cgaatattgt ggcggtggag
     4381 cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat ggacaaactt
     4441 ggcaatggcg gtaaagggat ttcgtggaat acccaggatg aaatcgattt cctcggcaaa
     4501 ctcaattaca ccaagcgtga tggcccggca caggggcgtc cgctgattga caccgccatt
     4561 gacgcttcag aagtgattct ggcactggca ccggaaacca acggtcatgt tgcagtcaaa
     4621 gcgtggcagg cgctgggcga gatcaccggg cgcgaacata cccatctggc gctgcacaaa
     4681 gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat tatctccagc
     4741 cccacatggt ccggtctgga aagcgatcac gtatcctata acgcgggata caccaacgtt
     4801 catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca ggatcatccg
     4861 tggatgcgtg cttttggtga aagcctggtg gcttatcgcc cgcctatcga cacccgtagc
     4921 gtcagtgaga tgcgccagat accgccaaac ggcttccagg aaaaagcact taacttcctg
     4981 acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct aatgctgacg
     5041 ctctctcgcg gtggaccgat tgtctggatc agcgaaacag atgcccgaga actgaccatt
     5101 gtcgataacg actgggtgga agtatttaac gccaacggcg cgctgactgc ccgcgcggtg
     5161 gtcagccaac gtgtaccgcc gggcatgacc atgatgtatc acgcccagga acgcattatg
     5221 aatattcctg gttcggaagt aactggcatg cgcggcggca ttcataactc ggtcacccgc
     5281 gtttgcccga aaccgacgca tatgattggc ggttacgcgc agctggcctg gggctttaac
     5341 tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa gatgaagaac
     5401 gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa atgaaaatac
     5461 gttcgcaagt cggcatggtg cttaacctcg ataaatgtat cggctgccat acctgttcgg
     5521 tgacctgtaa caacgtctgg accggacgcg aaggcatgga gtacgcatgg tttaacaacg
     5581 tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa gagtggcaag
     5641 gcggctgggt ccgcgatgtg aatggcaaga tccgtccgcg tctgggcagc aagatgggcg
     5701 taataaccaa aatcttcgcc aacccggtgg tgccgcagca gattgatgat tactacgaac
     5761 ctttcacctt cgactacgaa catttgcata gcacaccgga aggcaaacat atccctactg
     5821 ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg ggcccaaact
     5881 gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac ttcgaggcca
     5941 tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg ccgcgcctgt
     6001 gcgaacactg cctcaatccc agctgcgtgg cgacctgccc aagcggcgct atctacaaac
     6061 gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg cgtttgtgca
     6121 ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag tcagaaaaat
     6181 gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca gaaacctgcg
     6241 tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatc gaggaagcgt
     6301 cgagcaccga gcgcgaagtt gacctcta
//
LOCUS       DBKELA010000162         6258 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.162, whole genome shotgun sequence.
ACCESSION   DBKELA010000162 DBKELA010000000
VERSION     DBKELA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.162"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..656)
                     /locus_tag="ACUQR0_003786"
     CDS             complement(<1..656)
                     /locus_tag="ACUQR0_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HHV9110635.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     "
     gene            complement(646..2403)
                     /locus_tag="ACUQR0_003787"
     CDS             complement(646..2403)
                     /locus_tag="ACUQR0_003787"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HHV9110636.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2419..3314)
                     /locus_tag="ACUQR0_003788"
                     /pseudo
     CDS             complement(2419..3314)
                     /locus_tag="ACUQR0_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(3314..3919)
                     /locus_tag="ACUQR0_003789"
     CDS             complement(3314..3919)
                     /locus_tag="ACUQR0_003789"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HHV9110637.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4090..>6258)
                     /locus_tag="ACUQR0_003790"
     CDS             complement(4090..>6258)
                     /locus_tag="ACUQR0_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HHV9110638.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1834 a         1566 c         1312 g         1546 t
ORIGIN      
        1 attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac gggcaccatc
       61 cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg atgctgccat
      121 gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt acgccacctc
      181 acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat atgcagcgaa
      241 ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca tcctgtcacc
      301 ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa accgcagcag
      361 gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa tgtcgagcta
      421 caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac aatagtccag
      481 ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc atggctgtta
      541 aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa aaacggggcc
      601 agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt tatcacgacg
      661 caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc atttatcaaa
      721 tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca ttacccacgg
      781 tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg
      841 ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac ctgcgagtga
      901 gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa tcgccagcgt
      961 aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct gttcaaaacg
     1021 cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa catttaactc
     1081 actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg catccagtac
     1141 atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt tggcgtggag
     1201 atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc gaataccgcg
     1261 ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt tactgccttg
     1321 cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg tactcattcc
     1381 gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca taaagcgata
     1441 cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc atcgatccgc
     1501 tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt tagcatataa
     1561 gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt cacccaatgt
     1621 gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta atctctgata
     1681 aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca gaagttgcgt
     1741 ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat agagatcgag
     1801 caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac tcgccacccg
     1861 ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa atagaccacg
     1921 cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt taaccttaaa
     1981 ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa cccatgtggc
     2041 aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc cgacctggct
     2101 gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca taagagacgg
     2161 gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg cataaaaagc
     2221 agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat gttcatgccc
     2281 gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc agtgccgata
     2341 atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc gtgaattttc
     2401 cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa ataccggagc
     2461 ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta atagttttcc
     2521 actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc
     2581 actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg gtgtcagcaa
     2641 cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca gaccttccag
     2701 cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt tcccccataa
     2761 atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca gtaacgcccc
     2821 gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca aggcaaaaac
     2881 ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg aggggtcacc
     2941 cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa ataacagaaa
     3001 cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc aatataacca
     3061 acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa aacttatcag
     3121 cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa aacaaatgac
     3181 gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct caggtaaccg
     3241 ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag tgttgccaga
     3301 gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa cgattaatcg
     3361 cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat tgcatccact
     3421 gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga tcacttttgc
     3481 caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact
     3541 cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt tccggtaaaa
     3601 ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca ttcaaaatcg
     3661 cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc gcattgagcg
     3721 ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt gccaccaaca
     3781 ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg tgattcgctg
     3841 taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga aagagcggtt
     3901 ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat ggtgcatcaa
     3961 ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat atatcaaggt
     4021 attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta aaaattatta
     4081 cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta ttgcgcgcgt
     4141 cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa gctctaacgc
     4201 cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc ggggattatt
     4261 catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag agggggtgtg
     4321 gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca gtgtactcca
     4381 ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat cggggtcgag
     4441 tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt cctgcctcag
     4501 aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct tgagaacaac
     4561 agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt cttccaaacg
     4621 tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc taatcagctc
     4681 agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg gattattttt
     4741 ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca gatactgatt
     4801 tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc gttgtaaagc
     4861 agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg attttttctc
     4921 catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata acacacgtat
     4981 tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt cactctcttg
     5041 tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga acgctaagct
     5101 tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt aagtgagttg
     5161 tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc catcaataat
     5221 ggattcgggc aataaccacg catctttata aagctcctga atgatcatat cccaatctct
     5281 tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa gattcaacgg
     5341 accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga gcggttcacg
     5401 gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca actccagaat
     5461 ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat tgctattaat
     5521 aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc gcattatggc
     5581 aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt catccagcat
     5641 ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt cttttttcat
     5701 ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt ctttcagagc
     5761 atcttcatca tttattagac cccaataata tgcatcagga tttattatca tcagcgcttt
     5821 tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac tatttgtcga
     5881 agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat taatactccc
     5941 cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac ttgcatttat
     6001 cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca cgttaccgag
     6061 aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg tcaaattgcc
     6121 aagaacaatc aatttttgat tactctctat attaccttct accactaaat cgccacgatg
     6181 cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac tgttgatagc
     6241 ttgttcaact tgttgttt
//
LOCUS       DBKELA010000163         6254 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.163, whole genome shotgun sequence.
ACCESSION   DBKELA010000163 DBKELA010000000
VERSION     DBKELA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.163"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACUQR0_003791"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACUQR0_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HHV9110639.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACUQR0_003792"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACUQR0_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HHV9110640.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACUQR0_003793"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACUQR0_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="ACUQR0_003794"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="ACUQR0_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1657 a         1456 c         1477 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atga
//
LOCUS       DBKELA010000164         6213 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.164, whole genome shotgun sequence.
ACCESSION   DBKELA010000164 DBKELA010000000
VERSION     DBKELA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.164"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..378
                     /locus_tag="ACUQR0_003795"
     CDS             <1..378
                     /locus_tag="ACUQR0_003795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110641.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACUQR0_003796"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACUQR0_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HHV9110642.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACUQR0_003797"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACUQR0_003797"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HHV9110643.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACUQR0_003798"
     CDS             complement(1787..2476)
                     /locus_tag="ACUQR0_003798"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HHV9110644.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACUQR0_003799"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACUQR0_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HHV9110645.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACUQR0_003800"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACUQR0_003800"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HHV9110646.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACUQR0_003801"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACUQR0_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HHV9110647.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1577 a         1688 c         1546 g         1402 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacacgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg aca
//
LOCUS       DBKELA010000165         6057 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.165, whole genome shotgun sequence.
ACCESSION   DBKELA010000165 DBKELA010000000
VERSION     DBKELA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.165"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(16..99)
                     /locus_tag="ACUQR0_003802"
                     /pseudo
     CDS             complement(16..99)
                     /locus_tag="ACUQR0_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..1409
                     /gene="torT"
                     /locus_tag="ACUQR0_003803"
     CDS             381..1409
                     /gene="torT"
                     /locus_tag="ACUQR0_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HHV9110648.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1382..2074)
                     /gene="torR"
                     /locus_tag="ACUQR0_003804"
     CDS             complement(1382..2074)
                     /gene="torR"
                     /locus_tag="ACUQR0_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HHV9110649.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2161..3333
                     /gene="torC"
                     /locus_tag="ACUQR0_003805"
     CDS             2161..3333
                     /gene="torC"
                     /locus_tag="ACUQR0_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HHV9110650.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3333..5873
                     /gene="torA"
                     /locus_tag="ACUQR0_003806"
     CDS             3333..5873
                     /gene="torA"
                     /locus_tag="ACUQR0_003806"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HHV9110651.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5870..>6057
                     /gene="torD"
                     /locus_tag="ACUQR0_003807"
     CDS             5870..>6057
                     /gene="torD"
                     /locus_tag="ACUQR0_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HHV9110652.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1471 a         1516 c         1679 g         1391 t
ORIGIN      
        1 ccatttcggt cccagttagg acgctttctc atcaagccag tccgcccacc actgcatcat
       61 ctccctacgc ttatcgagat actgagcatg gttgtaaatt caattaaccc agtgcgttga
      121 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
      181 aataccatct aagtggttga ttcatagtga ctggatatgt tgtgttttgt cgcgtttttc
      241 agtctattat ttaatcatta agtaatctaa tgctatgatt tttaaattat ttatgtgatt
      301 ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg aatgcatcta gcataaagcc
      361 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
      421 tgtcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
      481 aagcctctat gccgcttaaa gccaaacgcg tatggaaact gtgcgcgctt tatcccagcc
      541 tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
      601 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
      661 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt
      721 catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg gtaaatgctc
      781 ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg ggctatcaac
      841 ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg ctgatgcccg
      901 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
      961 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgca
     1021 acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc ggaacggcca
     1081 ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt accgtggtgt
     1141 cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg attatggctg
     1201 ccagcgatca aatggtctgg cagggggaac tggcagttga gcaggccatc aggcaattac
     1261 aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg ccgaaaaatg
     1321 ccgaccgaga acatatccgc cgctcgctgt caccgggggg atttcgtccg gtctattttt
     1381 atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc agtaaatccg
     1441 cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg tcagggtttt
     1501 ccacccgacg cgcagaaagc atacgcagca gacgctcacg gctgagaatt tcgcccggat
     1561 tagtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcgccat
     1621 cccgctccag cgtatggcgc gacacattca ggcaataacc cgcaaagcga tagcagtagt
     1681 cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga tttttcaccc
     1741 gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca
     1801 gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc cccaccgttg
     1861 agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag ttgatatcca
     1921 gcagaattaa atctaccggc tgattctgca taatttcccg tagcccggca ccgctcgccg
     1981 taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc tgggtaaccg
     2041 gcttatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat
     2101 atctgttcat atttgctcat taagatcgct tcactaaacc ataattctac aggggttatt
     2161 atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt actggcgctg
     2221 gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt tgggatcaaa
     2281 gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa
     2341 tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt
     2401 catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag caacgacatt
     2461 taccagacct ttattgccca ctccattgat acacctgaaa aattcgaagc caaacgcgcg
     2521 gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac ctgtcgctcc
     2581 tgccataact acgatgcgat ggatcatgcc aggcagcatc ctgaagcggc acgtcagatg
     2641 aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat tgcccaccag
     2701 ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc cagtgctaat
     2761 gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc gaaaggcgat
     2821 aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa acgtgacggc
     2881 gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca gcgtgtactc
     2941 acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt gcagcagcag
     3001 gtgaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag caagttgcag
     3061 gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat ctgggcctat
     3121 gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga aatcgcccac
     3181 tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac cagtctcgat
     3241 aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga atgcgtctga caccgcaggt
     3301 aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc tttcaggcat
     3361 cacgtcggcg ttttctggca caacttggcg gcttaaccgt cgccgggatg ctggggccgt
     3421 cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc gactgaggct gtcatctcga
     3481 aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgatggtg aaggatggtc
     3541 gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg atcgccggat
     3601 tgccggatca cgtacacaac gcggcacgta ttcgttatcc gatggtacgc gtggactggc
     3661 tgcgtaagcg ccatctgagc gacacctccc agcgcggtga taaccgtttt gtgcgcgtga
     3721 gctgggatga agccctcgac atgttctatg aagaactgga acgcgtacag aaaactcacg
     3781 ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc cataacgctt
     3841 cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg ggcggagatt
     3901 actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt cggttcaatg gaagtgtatg
     3961 aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg ctgtggggct
     4021 ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat
     4081 attacgagca gctaaaagcg aaagtcgccg ccggtgaagt tgaggtcatc agcatcgatc
     4141 cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta
     4201 acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca tacgctgtac agtgaaaacc
     4261 tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc ctgccgtatc
     4321 tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca
     4381 ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcaaatta
     4441 ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg
     4501 ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggttttggc tggcactaca
     4561 acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt
     4621 cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcact attccgattg
     4681 cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg
     4741 taaaactgcc gccgctgaaa atgtgtattt ttgccggaac taacccattc catcgccatc
     4801 agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc gccatagata
     4861 accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc acgcagtttg
     4921 agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac
     4981 aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc gagctgtgcc
     5041 gtcgctttaa tcgtgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac
     5101 gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg ccagcgtttg
     5161 atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg tttgttcgcc
     5221 accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg
     5281 agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt
     5341 ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat ccgttgcatc
     5401 tgcaatctgt gcatccggat ttccgacttc actcgcagct atgtgagtcg gaaacgctgc
     5461 gtcagcaata tacggtagcg ggtaaagagc cagtgttcat taacccgcag gatgccagcg
     5521 cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg
     5581 caggagcagt agtttctgac cgctatgcac ccggcgtggc gcgaattcac gaaggggcat
     5641 ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg
     5701 tgctgaccat cgacatcggg acttcgcagc tggcgcaggc ggccagtgcg cacactacgc
     5761 tggtggaaat tgagaagtac aatggaacag tggagcaggt gacggcgttt aacggccccg
     5821 tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct
     5881 gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact
     5941 ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt tttcgttgct
     6001 gaaaagcgaa ccgccgctca ctgcggcggt gaatgatctg gaaaacagta ttgccac
//
LOCUS       DBKELA010000166         6055 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.166, whole genome shotgun sequence.
ACCESSION   DBKELA010000166 DBKELA010000000
VERSION     DBKELA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.166"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            431..1339
                     /locus_tag="ACUQR0_003808"
     CDS             431..1339
                     /locus_tag="ACUQR0_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HHV9110653.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="ACUQR0_003809"
     CDS             complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="ACUQR0_003809"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHV9110654.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3654..4412
                     /gene="loiP"
                     /locus_tag="ACUQR0_003810"
                     /gene_synonym="yggG"
     CDS             3654..4412
                     /gene="loiP"
                     /locus_tag="ACUQR0_003810"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HHV9110655.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4618..5538)
                     /gene="speB"
                     /locus_tag="ACUQR0_003811"
     CDS             complement(4618..5538)
                     /gene="speB"
                     /locus_tag="ACUQR0_003811"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HHV9110656.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5674..>6055)
                     /locus_tag="ACUQR0_003812"
     CDS             complement(5674..>6055)
                     /locus_tag="ACUQR0_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110657.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1490 a         1576 c         1563 g         1426 t
ORIGIN      
        1 gctgaatgta taactgagat tgatgattct ggaaaataat cactaagccg cataaattat
       61 ccttatttta tttttagggt accgtccggc ggtaaaaata aaatactcgt gcggtttaat
      121 gaaagcaatt gggtacgcga gaagaaaaac cccacaaacc aaaataataa aaccttaaat
      181 aacaacaata tgaaattgat atttttaaag tcaactttgt atttgttatg ttcatcacaa
      241 aatcaacatt ttgtttgtta ttgtatttgt gatgcatatc tctatttatt tattgaatgt
      301 tcgaaaacat acttttctaa tgcaatgttt tttcgctata cccctttata gattctgaat
      361 tctcacctgt cggctgtttt ctcctcatct catgtgacat attcccatac tttactcaag
      421 gaagattgtc atgaaagcac ctgtttcatt aacctcattt cgcccacaaa aatctcttgc
      481 tatcgccatt ggcgtgcttg ccgtggttgt gctgccattt ctctcgtatt acaccgttaa
      541 tgaaggtgaa cgcggcattt tgctgcgcta cggaaaaatc gtaaaagttg ccgaaccggg
      601 gctgggtttt aagatccctt ttatggaaag cgtggagaaa atttccacgc gtaatcaggc
      661 tgtggtctat cagggattac aggcctacag ccgcgaccag caaccggcac aaatgacagt
      721 atcagtcagc ttccacatta aaccttcaga agcaggcgcg gtttatacca cctacaacac
      781 tattgaagca ttaaaagatc gtctaattgt ccgtcagttg ccaactcagc tggagaacat
      841 tttcggccaa tataccgcca tcagcgccgt acaggatcgt acaaaattgg ttcaggatct
      901 gcaaaatgcc atgcgcaaag ccgttgttgg gccagtggta attgacgggg tccagattga
      961 aaatattgat ttttctgacg cttatgaaaa gtcgattgaa aaccgaatga aagcagaagt
     1021 cgccattgcc acgcgtaagc aaaatctgga aacagagaaa atccaggcgc aaatagcggt
     1081 aactcaggcc caggcagaag cagacagtaa actggcggca gccaaagtag aagcggaaac
     1141 tattcgcgta agaggcgcgg cagaggctga aactattcgt ttaaaaagtg cggctgaagc
     1201 agaagcaatc cgcctgcgcg gtgaagctct gcgggataat cccggacttg tagccctcac
     1261 cactgccgaa cgttgggacg gtaaactgcc cgataccatg atccccggca gcacagttcc
     1321 gtttatctct actaagtaaa aaaagggccg ctttcgcggt ccttctcaca aaacgtctta
     1381 ctgattacag cagcgctttt gctttcgcaa caacgttatc aacagtgaag ccgaactctt
     1441 caaacagcag ctctgccgga gcagattcac cgaaggtggt catgccgacg atagcgccgt
     1501 tcaggccaac atacttgtac cagtagtcag caatacccgc ttctaccgca acgcgtgcag
     1561 taaccgcttt cggcagtacg gattcacggt aagccgcatc ctgcttgtca aatgcgtcgg
     1621 tagacggcat ggacaccacg cgcgctttca cgccttcggc agtcagtttt tcgtaggcag
     1681 caaccgccag ttcaacttca gaaccggtgg cgatgaagat cagttccggc tgaccagcac
     1741 agtctttcag cacataacca ccgcgcgcga tgtttgccag ctgctcttca gtacgttcct
     1801 gctgcgccag gttctgacgg gagaggataa gcgcagtcgg gccgtcctga cgctcaacgc
     1861 cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg
     1921 gggtcacgcg cagagaagcg acctgctcta ccggctggtg agtcgggcca tcttcgccca
     1981 gaccgatgga gtcgtgggtg taaaccatca cctggcgctg tttcatcagc gcagccatac
     2041 gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaagc
     2101 caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt
     2161 ggatgtagtt acctgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt
     2221 tagacggtgc caggtcagca gagccgccga ggaattcagg caacagcggg ccgaacgctt
     2281 cgatagcatt ctgcgacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag
     2341 cgataaactc tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc atacggcggg
     2401 taaattcagc cgcttcctgc ggataagctt tcgcgtaagc cgcaaacttc tcattccatg
     2461 cagattcttt cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga
     2521 tttcgaacgg tgcatatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc
     2581 ccagcggcgc accgtgggag tcgtgagtac cggctttgtt cggggaaccg aaaccgatga
     2641 tggttttgca catcagcagg gacggtttgt cagtcactgc gcgcgcttct tcaactgcgc
     2701 gtttgatgga tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt
     2761 cgaaacgcat tgctgtgtcg tcggtgaacc agccttcaac gtgaccatcg atagaaatac
     2821 cgttgtcatc gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa
     2881 cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt
     2941 cgacaatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca
     3001 taccgactgc gttggcaata ccctgaccca gcggaccggt ggtggtttcc acaccagcgg
     3061 tataacctac ttccgggtgg cccggagttt tagagtgcag ctgacggaag tttttcagtt
     3121 cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt
     3181 ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt
     3241 tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt
     3301 gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct
     3361 ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt
     3421 gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc
     3481 gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt
     3541 gcgcaatctg ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcgattca
     3601 cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa
     3661 ttcgcgcctt attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg
     3721 actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg
     3781 cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg
     3841 cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca
     3901 atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg
     3961 caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg acagataacg
     4021 aagtcgaagc ggtgatcggt cacgagatgg ggcacgtggc gttaggccat gtgaagaaag
     4081 ggatgcaggt ggcgcttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga
     4141 ttgtcggcag tttatctcaa tcacaacttg gtgatctggg cgagaaatta gtcaattcgc
     4201 aattctccca gcgtcaggaa gcagaagccg atgattattc ttacgatctt ctgcaccaac
     4261 gcggcatcag cccggcaggt cttgccacca gctttgaaaa actggcgaag ctggaagaag
     4321 gccgtcaaag ctcaatgttt gacgaccatc ctgcatccgc cgaacgtgcc cagcatattc
     4381 gcgatcgcat cagcgcggat gggattaagt aaagcctagt ggtgtcgaac gtaggtcaga
     4441 taaggcgttc acgccgcatc tgacattaat ggcacatttg ttaccttgtg cgcattgccg
     4501 gatgcgatgc tggcgcatct tatccggcct acgggtgccg aacgtaggtc ggataaggcg
     4561 ttcacgccgc atccgacatt aatggcacgt tttacccgtg cgcatcgcat ctggtgctta
     4621 ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg ccgcagccag
     4681 agcggtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat
     4741 gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca ggccgccaat
     4801 cactggcgta ccggttcccg gcgcgaaagc aggatccagg cagtcgatat caaaagtcag
     4861 gtaaaccggc atatcaccca caatctgttt cacctgggcg ataacgtcat ccacgctgcg
     4921 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa actcggtacg
     4981 aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa
     5041 catagtgccg tggtcaaatt cacaaccgtt cgcataggta tcggtgtggg cgtcaaagtg
     5101 taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
     5161 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
     5221 ctgcagcttt tcgctcatct cacgggcatc gccaaaggca tataccagat cgccgcagtc
     5281 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
     5341 cagattcgtc gaaacctgac ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga
     5401 agtggccata tcgaacggca cgccagtaat cacccagtct gcatcgctgt catacggctg
     5461 gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa accagtgagt tatcgtattg
     5521 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
     5581 gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg tgctaattat
     5641 cgccttaaat gggcgaggca tcaagcgagg ttgctatttc cgacacggtg gcggcgcata
     5701 gccattgtta tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt tttgcgccgg
     5761 gccttgtaat tcaaaggcgg caaagccgat taccccggta ttacgcacat cgccttgcgc
     5821 cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cagtatgttt
     5881 atctttgcgg aatcctttaa tcactaacga atctccggca ttcacaatat agccgttgtt
     5941 attcagcgaa ccttgcttgc cgttcagcac atccaggcca tcagccgtag caacgatctc
     6001 gtagttggtg ttacgactgt aattacggac atataattga taattcattc cgact
//
LOCUS       DBKELA010000167         6028 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.167, whole genome shotgun sequence.
ACCESSION   DBKELA010000167 DBKELA010000000
VERSION     DBKELA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.167"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACUQR0_003813"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACUQR0_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HHV9110658.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACUQR0_003814"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACUQR0_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HHV9110659.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACUQR0_003815"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACUQR0_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HHV9110660.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACUQR0_003816"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACUQR0_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HHV9110661.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACUQR0_003817"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACUQR0_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACUQR0_003818"
     CDS             2795..3100
                     /locus_tag="ACUQR0_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HHV9110662.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACUQR0_003819"
     CDS             3103..3507
                     /locus_tag="ACUQR0_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HHV9110663.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACUQR0_003820"
     CDS             3497..3796
                     /locus_tag="ACUQR0_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HHV9110664.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACUQR0_003821"
     CDS             3982..4278
                     /locus_tag="ACUQR0_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HHV9110665.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACUQR0_003822"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACUQR0_003822"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HHV9110666.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="ACUQR0_003823"
     CDS             5627..5995
                     /locus_tag="ACUQR0_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HHV9110667.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1524 a         1470 c         1519 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaatt
//
LOCUS       DBKELA010000168         5981 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.168, whole genome shotgun sequence.
ACCESSION   DBKELA010000168 DBKELA010000000
VERSION     DBKELA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.168"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..262)
                     /locus_tag="ACUQR0_003824"
     CDS             complement(<1..262)
                     /locus_tag="ACUQR0_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HHV9110668.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            483..911
                     /gene="uspG"
                     /locus_tag="ACUQR0_003825"
     CDS             483..911
                     /gene="uspG"
                     /locus_tag="ACUQR0_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HHV9110669.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="ACUQR0_003826"
     CDS             complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="ACUQR0_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HHV9110670.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="ACUQR0_003827"
     CDS             complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="ACUQR0_003827"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HHV9110671.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3777..4523
                     /gene="dsbG"
                     /locus_tag="ACUQR0_003828"
     CDS             3777..4523
                     /gene="dsbG"
                     /locus_tag="ACUQR0_003828"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HHV9110672.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4733..5635
                     /gene="citR"
                     /locus_tag="ACUQR0_003829"
     CDS             4733..5635
                     /gene="citR"
                     /locus_tag="ACUQR0_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor CitR"
                     /protein_id="HHV9110673.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5782..5981
                     /locus_tag="ACUQR0_003830"
                     /pseudo
     CDS             5782..5981
                     /locus_tag="ACUQR0_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1605 a         1440 c         1347 g         1589 t
ORIGIN      
        1 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
       61 caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt
      121 tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa
      181 tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc
      241 cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt
      301 aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt
      361 gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata
      421 tcagggataa atattataat catggagaat gatttgttca tgattaacag ggagaaaggg
      481 ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag
      541 ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac
      601 tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag
      661 agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg
      721 atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt
      781 tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga
      841 cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg
      901 tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt
      961 tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat
     1021 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     1081 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     1141 gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg
     1201 cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca
     1261 aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga
     1321 ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     1381 acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     1441 aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg
     1501 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggtcc gtcgcagtgc
     1561 gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc
     1621 cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
     1681 tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg
     1741 tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc
     1801 ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
     1861 ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
     1921 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
     1981 tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc
     2041 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
     2101 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
     2161 ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc
     2221 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
     2281 tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac
     2341 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
     2401 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
     2461 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
     2521 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
     2581 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
     2641 gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
     2701 ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga
     2761 atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat
     2821 ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt
     2881 tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg
     2941 agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac
     3001 ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc
     3061 ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat
     3121 cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg
     3181 agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc
     3241 agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa
     3301 gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa
     3361 tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt
     3421 gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat
     3481 caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc
     3541 agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct
     3601 ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac
     3661 atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga
     3721 cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt
     3781 taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt
     3841 ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg
     3901 gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc
     3961 ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta
     4021 acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg
     4081 aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct
     4141 ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt
     4201 ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga
     4261 cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct
     4321 ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt
     4381 taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt
     4441 acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta
     4501 atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt
     4561 ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta
     4621 gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa
     4681 acattgacta agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa
     4741 tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca
     4801 gcatttaagc attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca
     4861 gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg
     4921 tatcgcgccg acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg
     4981 ccttgaacaa acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta
     5041 cggccctcag ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga
     5101 tgcctctgtc gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact
     5161 gttggttcac cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt
     5221 catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg
     5281 aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc
     5341 caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa
     5401 aatttcattt cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt
     5461 actgggaata gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga
     5521 gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc
     5581 tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat
     5641 aactatagac agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa
     5701 cactctgctt tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta
     5761 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
     5821 gaagctgcac gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca
     5881 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa
     5941 atgggcaaaa aaatctgcgt tttgtttatc aactgggagg c
//
LOCUS       DBKELA010000169         5783 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.170, whole genome shotgun sequence.
ACCESSION   DBKELA010000169 DBKELA010000000
VERSION     DBKELA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.170"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..436
                     /locus_tag="ACUQR0_003831"
     CDS             <1..436
                     /locus_tag="ACUQR0_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HHV9110674.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQF
                     AVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="ACUQR0_003832"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="ACUQR0_003832"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HHV9110675.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="ACUQR0_003833"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="ACUQR0_003833"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HHV9110676.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="ACUQR0_003834"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="ACUQR0_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HHV9110677.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="ACUQR0_003835"
     CDS             complement(4438..5145)
                     /locus_tag="ACUQR0_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HHV9110678.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..>5783)
                     /gene="dapF"
                     /locus_tag="ACUQR0_003836"
     CDS             complement(5142..>5783)
                     /gene="dapF"
                     /locus_tag="ACUQR0_003836"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HHV9110679.1"
                     /translation="IFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMV
                     LTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHCVIQ
                     VDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGA
                     CAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
BASE COUNT         1266 a         1676 c         1508 g         1333 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aat
//
LOCUS       DBKELA010000170         5749 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.171, whole genome shotgun sequence.
ACCESSION   DBKELA010000170 DBKELA010000000
VERSION     DBKELA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.171"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..403)
                     /locus_tag="ACUQR0_003837"
     CDS             complement(<1..403)
                     /locus_tag="ACUQR0_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HHV9110680.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(548..1051)
                     /gene="cheW"
                     /locus_tag="ACUQR0_003838"
     CDS             complement(548..1051)
                     /gene="cheW"
                     /locus_tag="ACUQR0_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HHV9110681.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1072..3036)
                     /gene="cheA"
                     /locus_tag="ACUQR0_003839"
     CDS             complement(1072..3036)
                     /gene="cheA"
                     /locus_tag="ACUQR0_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HHV9110682.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3041..3967)
                     /gene="motB"
                     /locus_tag="ACUQR0_003840"
     CDS             complement(3041..3967)
                     /gene="motB"
                     /locus_tag="ACUQR0_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HHV9110683.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3964..4851)
                     /gene="motA"
                     /locus_tag="ACUQR0_003841"
     CDS             complement(3964..4851)
                     /gene="motA"
                     /locus_tag="ACUQR0_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HHV9110684.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4978..5556)
                     /gene="flhC"
                     /locus_tag="ACUQR0_003842"
     CDS             complement(4978..5556)
                     /gene="flhC"
                     /locus_tag="ACUQR0_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HHV9110685.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5559..>5749)
                     /locus_tag="ACUQR0_003843"
     CDS             complement(5559..>5749)
                     /locus_tag="ACUQR0_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HHV9110686.1"
                     /translation="VKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLST
                     RLLNDVNQPEEALRKKRA"
BASE COUNT         1339 a         1552 c         1403 g         1455 t
ORIGIN      
        1 ttaacgctgt gtaatagttt ttatattttt catcaaaatt acggctggta gcgaccattt
       61 caggtaacgg tgccatgctt ttgaattttt tataatgcgt cgcggcctgg gccaatgttt
      121 tcctagcgct atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca
      181 tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg
      241 acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat
      301 ggtgaaggga agaaaaaaac aaactgccgg aaataagctg taacagtgcg aataccccca
      361 gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa
      421 acaagttgat ctcgttatcg gcaagaaggg gggaaacttt attgctgatg ccacccgccg
      481 cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc
      541 gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca
      601 gcagtttttc gatgttcacc agaatcaaca tacggtcgcc cagtgcgccc agtccagtga
      661 gatattctgt tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc tccgccgtca
      721 atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca
      781 ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta
      841 agtcaacaat cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg
      901 tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa
      961 taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg
     1021 ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc
     1081 ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag
     1141 tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt
     1201 actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc
     1261 gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc
     1321 gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc
     1381 cagcacccgc tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc
     1441 cataacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc
     1501 gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga
     1561 ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac
     1621 gccgcgcccg gagacgtcgg tgacctgctc tgccgtggag aagcctggtg caaatatcag
     1681 cattgcaact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt
     1741 tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt
     1801 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
     1861 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
     1921 gtcgataatg cgttctatca ggctcttgtc gagctcggtg gaactgccca ccagcgtcag
     1981 ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa
     2041 gacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
     2101 acgttgtaac tgccccatgc tggtaatcaa atcaccatga ttaaccgggt ccagttcgct
     2161 ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg
     2221 atcaaccttt tctaccgcta cacggatgct ggtggattca ctgctgcgcg tcgttttttc
     2281 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcacag gtggggtagc
     2341 tatttttggc gaaacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
     2401 gagtaccgct gtgatgtcat cttcggcgat atcgcccggt aatattgccg agagagaatc
     2461 cgcccctttc accacgtcat ttaacgttgt cagatgcccc agctcttctt ccagcaggtc
     2521 gacttcgccg gccttcaggc gcgaaaggat aattcgtcgc ggcaactgac tgcgactttg
     2581 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc
     2641 gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcgt agctggcggc
     2701 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
     2761 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag
     2821 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc
     2881 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
     2941 cggctgcaaa accagcagat gctgctccat atcagccaac agttcgtccg cttcatcaaa
     3001 aaatgtctga taaaaatcgc ttatatcgat gctcacgctg tcacctcggt tcggctgatg
     3061 gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttctagggcg cttactggct
     3121 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
     3181 acaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
     3241 ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc
     3301 ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctgtaacct ttctcaccgc
     3361 tggcgtaggg aaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca
     3421 gcacaggcgc gatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg
     3481 ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
     3541 gatcgatttt gagatggaga cgtaacgccc gcagtttcgg atcggactct atcaactggt
     3601 cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga
     3661 tgttcggctg cttattcact tccccctggc tctgggtgta atcatcacca ccgccgggaa
     3721 ttgggctttc actattggaa atgcgatcgc cgcccgtaac cgctgtcgcc agtggagtcc
     3781 ggaagtactc cgcaatctga atcaactctt ttgggctgga gatggagatc agccacatca
     3841 ccaaaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat
     3901 gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
     3961 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
     4021 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
     4081 ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat
     4141 cattttgctg gtttcggcgc ttttctgacg taaaacactc gctagtgggg aaataaatcc
     4201 gtaagccaat aaaataccga ggaaagtccc caccatcgca tgtgcgataa gcacccccag
     4261 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg ccccccatta cagccgcgac
     4321 aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc
     4381 ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat
     4441 gtgaccgctg ataatcaggc gcagataatc gacgataaaa tcaagcatga cgctatccgc
     4501 gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
     4561 cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag
     4621 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgctttcag
     4681 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
     4741 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
     4801 accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc
     4861 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggaa
     4921 aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgccacacc gtatcagtta
     4981 aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag gattctggga
     5041 aagtttacgt ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc
     5101 aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg
     5161 cagtaatcca ctttcaacaa accgcaccaa tgtcctggca cgggttaatg ccagcagtgg
     5221 tccttcttct gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc
     5281 gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc
     5341 atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg
     5401 cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt
     5461 ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat
     5521 atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc
     5581 gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga
     5641 atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg
     5701 tcaaaacgga agtgacaaac cagctgattg gtttctgcca gcttaacca
//
LOCUS       DBKELA010000171         5702 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.172, whole genome shotgun sequence.
ACCESSION   DBKELA010000171 DBKELA010000000
VERSION     DBKELA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.172"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..219)
                     /locus_tag="ACUQR0_003844"
     CDS             complement(<1..219)
                     /locus_tag="ACUQR0_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HHV9110687.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /gene="ltnD"
                     /locus_tag="ACUQR0_003845"
     CDS             complement(216..1124)
                     /gene="ltnD"
                     /locus_tag="ACUQR0_003845"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HHV9110688.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /gene="ygbI"
                     /locus_tag="ACUQR0_003846"
                     /pseudo
     CDS             1320..2087
                     /gene="ygbI"
                     /locus_tag="ACUQR0_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="ACUQR0_003847"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="ACUQR0_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="ACUQR0_003848"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="ACUQR0_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HHV9110689.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="ACUQR0_003849"
     CDS             5533..>5702
                     /locus_tag="ACUQR0_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110690.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DBKELA010000172         5684 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.173, whole genome shotgun sequence.
ACCESSION   DBKELA010000172 DBKELA010000000
VERSION     DBKELA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.173"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..176)
                     /locus_tag="ACUQR0_003850"
     CDS             complement(<1..176)
                     /locus_tag="ACUQR0_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110691.1"
                     /translation="MRIIFLCKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWS"
     gene            complement(601..744)
                     /gene="ypaB"
                     /locus_tag="ACUQR0_003851"
     CDS             complement(601..744)
                     /gene="ypaB"
                     /locus_tag="ACUQR0_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HHV9110692.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="ACUQR0_003852"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="ACUQR0_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHV9110693.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="ACUQR0_003853"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="ACUQR0_003853"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHV9110694.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="ACUQR0_003854"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="ACUQR0_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HHV9110695.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="ACUQR0_003855"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="ACUQR0_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HHV9110696.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5559..>5684)
                     /locus_tag="ACUQR0_003856"
     CDS             complement(5559..>5684)
                     /locus_tag="ACUQR0_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110697.1"
                     /translation="RLIKFTFYLLGALLVVVAGGIYALQMKGKSGVTPVAAKEIH"
BASE COUNT         1456 a         1488 c         1350 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtcg
//
LOCUS       DBKELA010000173         5582 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.175, whole genome shotgun sequence.
ACCESSION   DBKELA010000173 DBKELA010000000
VERSION     DBKELA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.175"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..243
                     /locus_tag="ACUQR0_003857"
     CDS             <1..243
                     /locus_tag="ACUQR0_003857"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HHV9110698.1"
                     /translation="PLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVLQWKNHVL
                     HAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            236..622
                     /locus_tag="ACUQR0_003858"
     CDS             236..622
                     /locus_tag="ACUQR0_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HHV9110699.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            630..1316
                     /locus_tag="ACUQR0_003859"
     CDS             630..1316
                     /locus_tag="ACUQR0_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHV9110700.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="ACUQR0_003860"
     CDS             complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="ACUQR0_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HHV9110701.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1999..3204
                     /gene="tet(B)"
                     /locus_tag="ACUQR0_003861"
     CDS             1999..3204
                     /gene="tet(B)"
                     /locus_tag="ACUQR0_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HHV9110702.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="ACUQR0_003862"
     CDS             complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="ACUQR0_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HHV9110703.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3998..4393
                     /locus_tag="ACUQR0_003863"
                     /pseudo
     CDS             3998..4393
                     /locus_tag="ACUQR0_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4424..>5582)
                     /locus_tag="ACUQR0_003864"
     CDS             complement(4424..>5582)
                     /locus_tag="ACUQR0_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HHV9110704.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1030 c         1215 g         1795 t
ORIGIN      
        1 ccactattaa gtcatgtagc tggttttatt tcaattgaac gttttcaaag tctagctaca
       61 gaaggaaaaa tgttatcgct atcttggtgg gaaaacgaat acgcagttct gcaatggaaa
      121 aatcatgttt tacatgcgaa agctcaacaa gaagggcgag agtcaatatt tgatttttac
      181 aaaattagta ttgctcatat tactcgcgaa tattcattta aaaaggacaa ggataatgtt
      241 tgatgttcac gttgttttag ataatcaaat aggacaatta gcattactag gaaaaacatt
      301 aggtaataaa ggtattggat tggaaggggg agggatattt acggttggtg atgaatgcca
      361 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      421 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      481 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      541 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      601 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      661 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      721 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      781 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      841 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      901 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      961 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
     1021 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
     1081 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
     1141 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
     1201 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1261 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1321 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1381 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1441 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1501 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1561 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1621 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1681 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1741 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1801 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1861 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1921 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1981 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     2041 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     2101 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     2161 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     2221 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2281 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2341 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2401 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2461 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2521 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2581 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2641 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2701 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2761 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2821 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2881 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2941 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     3001 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     3061 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     3121 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     3181 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3241 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     3301 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     3361 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     3421 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     3481 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     3541 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     3601 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     3661 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     3721 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     3781 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     3841 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     3901 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     3961 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     4021 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     4081 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     4141 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     4201 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     4261 gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgattccca
     4321 acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt attatcggcg
     4381 taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat ttccccaaag
     4441 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc
     4501 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta
     4561 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat
     4621 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa
     4681 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca
     4741 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg
     4801 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg
     4861 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc
     4921 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc
     4981 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt
     5041 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt
     5101 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa
     5161 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag
     5221 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac
     5281 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc
     5341 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt
     5401 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga
     5461 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag
     5521 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta
     5581 at
//
LOCUS       DBKELA010000174         5558 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.176, whole genome shotgun sequence.
ACCESSION   DBKELA010000174 DBKELA010000000
VERSION     DBKELA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.176"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..642
                     /locus_tag="ACUQR0_003865"
     CDS             <1..642
                     /locus_tag="ACUQR0_003865"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HHV9110705.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACUQR0_003866"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACUQR0_003866"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HHV9110706.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="ACUQR0_003867"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="ACUQR0_003867"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HHV9110707.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagata
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagctat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaa
//
LOCUS       DBKELA010000175         5494 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.177, whole genome shotgun sequence.
ACCESSION   DBKELA010000175 DBKELA010000000
VERSION     DBKELA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.177"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..190)
                     /locus_tag="ACUQR0_003868"
     CDS             complement(<1..190)
                     /locus_tag="ACUQR0_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110708.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDIT"
     gene            331..537
                     /locus_tag="ACUQR0_003869"
     CDS             331..537
                     /locus_tag="ACUQR0_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9110709.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            632..1234
                     /locus_tag="ACUQR0_003870"
     CDS             632..1234
                     /locus_tag="ACUQR0_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110710.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(1140..1336)
                     /locus_tag="ACUQR0_003871"
                     /pseudo
     CDS             complement(1140..1336)
                     /locus_tag="ACUQR0_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333284.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2434..3351
                     /locus_tag="ACUQR0_003872"
     CDS             2434..3351
                     /locus_tag="ACUQR0_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110711.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            3436..4308
                     /locus_tag="ACUQR0_003873"
     CDS             3436..4308
                     /locus_tag="ACUQR0_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HHV9110712.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            4526..>5494
                     /locus_tag="ACUQR0_003874"
     CDS             4526..>5494
                     /locus_tag="ACUQR0_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HHV9110713.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNS"
BASE COUNT         1410 a         1390 c         1333 g         1361 t
ORIGIN      
        1 cggtgatatc agtctcctcc tcgccaggaa cgggctgact cactgtaact acctccgaaa
       61 acaactctgc aattatcaga acgcctgctt ctctccctgt cctcacgaaa actatcccct
      121 ctttatcgcg cgtgcgtgcg gaagcatctt ttcgcaacaa ccacccggta taccggtacg
      181 gctcagccac cgcaacctcc ccgtttatct cctggcggtc acattcccga ttatttttta
      241 cgtttctccc cggttgttat gccggtgaag gtggcgcgct gttttcatga ccacaccggc
      301 tggtttaaca acatccggag gaacattctc atggctaccc cagtttcact gatggatgac
      361 cagatggtcg acatggcatt tatcactcaa ctgaccggcc tgacagataa gtggttttac
      421 aggctcatca gggatggggc ctttccggct cccatcaagc tgggccgcag ctcccgctgg
      481 ctgaaaagtg aagtggaagc ctggctacag gcgcgtattg cacagccccg cccgtaattt
      541 ctgaccgtta tccgttcacc cgcagcagac gtaatccccg gcctgccggc ggcattctgc
      601 tgcctgttat atccccgtga ggaatatgaa aatgaaacaa cagtatcaga cccgctacga
      661 atggctccac gaaagctacc agaaatggct gaccggcttc acccggcacg ccgtatcctg
      721 gggcgtgtgt catccgaata tctactactt ccataatctg acgcccggtt gggggtcatt
      781 caatggtgaa aaaccggaga ttgccatcgt accccagagc ctgcaccggc tgatttatgg
      841 ccctgacaaa cgggccacgc cgcccctgga tgatgatctg atagtgaatt tatgcaccag
      901 tgaacatctg ctggttcatc atccgatgct ggaaggcatt ctcctgtctg aatgtgaacg
      961 tctgaggcag cgttctctgg caaataaact gatcagcctc ttccgtcagt ttggtggcac
     1021 ggagttgcgc ctcaaactgg tctggctttg ctggcttgat ttaatgaccg gaaacagcct
     1081 tgaagactgg aaagagaacc tgaaacggaa atcagaaaaa gagctggagg aatggatcat
     1141 caaccgtcag aggcagagcg cagcactgac ggatctgatg gatcagtacg tgctcctggc
     1201 ataccgcaca acggttgacg atagccgcaa ctgatgccgg ttgcttccgg aaggtgtgac
     1261 aaaatacgga aatgatgatg ctcccggccc ccatgctcac ctgcagagcg tgttcgtcgt
     1321 catcatactg agccatgacg ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca
     1381 ccaccagagg agccacggac agggatgttc tttacagccc gatacacgcc aggttctcag
     1441 cagactgaat gtgcaggtgc ggttattcac cagaaatggc atgctgcggt acatacccat
     1501 cctggtgaca atatgtacgt ggcgacagcg atcccggcac tgtggaagag actcactcag
     1561 cctgcaggat attctttccg gcatccggga atgtccgcct gaatgcaaac aggatgatat
     1621 ttgaatccgt aatactgttc tttcatcata aataatttat gcagatacag ttaaccgtta
     1681 cagtagccgg tttgttttac cgggaaataa tatccgtcag actgataccg gaacatatct
     1741 gaaacacgtt atgatgtttt gttatcagaa atgttgtata ttcttatccg tggcgacagt
     1801 ttaatgccct ctggtatcac ctgataatgt atatcctgcg atgattcgtt atatttacgg
     1861 gacacgcctc ttttcattgt gctccttttc agacttcatt ttccgtaagt gttccccggc
     1921 ataaaggagg tacctttctg gcgtttataa ctctctttat cggtgacgcc ggtaatacag
     1981 ggaataccac accgacaaac gaggtattaa cagacgatat ttcagtgtcc ataatttttc
     2041 tgtccggtta aataacgtca gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc
     2101 ataccgggtt acaacgtaaa aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac
     2161 atgtttacct ttcaataccc cctgtctgca gtctccactc ttccatcctc ttaagctacg
     2221 tcggccacat gagtgcactt ccatgataac aggtattcct gtcagcataa cgaagccggt
     2281 taccttccct tcctcctgca tttcctgtca gaatgactat acagcgcgca gattgccttt
     2341 ttattgacca tcttaatata ccgttataaa cttcttttta tcatattatt atcaccttat
     2401 tatatttaat ttttaagcag tcggagaaag aggatggttc agaaaatttt gtcggataag
     2461 gttatgaatg aaagaacaaa tgcgtattac tcttattatc ttggagagcg taatatctcc
     2521 gtactgcccc ttaatgttta tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac
     2581 agagaaaatc tgaacatcac actttcagat tttgaacttg aacaaataat ttctggtatg
     2641 cgtctgaagg ccctggcctt cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt
     2701 gaacgcgcat gcttattttc ctggtatctg ttgatgcagt ttatccagaa taacagggca
     2761 aaaataagcg cagacttgct tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa
     2821 gggaatgcct tcccgtcgga ctcatcgaca cagttcagac aaatactcag agttctcgat
     2881 attctttctg ataagaattt gcgggatgag tggataatcc agaccaaaga cagatggatg
     2941 cgggcattta aatcaaagag tcctttcagt tacctcctcc cggagaacga acatgaatgc
     3001 atatggacat ggaattattt gaagggaaaa aatattgcac tggaaaaact ggccagtttt
     3061 cctggttctg ctgatattta ccacgcaatt cacctttcat ttgacatatg ggttacctgt
     3121 ccactcacct cacctgacga tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg
     3181 cagcgcaagt acaaaaaaat gcaggaagat aaagtcaatg tacagttttt tcttgacgca
     3241 gaaaccaggg cgcagctcaa agaattatcg agggtcagaa gactcagtac cggcgaaatg
     3301 ctgcatgacc tgattgttga agagtataaa aggtaccgac attccagata atttcagaca
     3361 tatattatct ccgtgagtcc cccgccacct ttccggtgtg cgggggtttt gtcttttttc
     3421 accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa accgctctcc
     3481 tccctgcctt actcactttc ccgtcacatc ctggaacatc tgcgcaaact caccagtcac
     3541 gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact ctgtaatgca
     3601 ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg ggaagtgcag
     3661 cgtttccgtc tgagtggcca tggtcacagc atggttatca ctgacctgcc cggggtgggc
     3721 gagagccggg acagagatgc agagtatgaa gccctgtacc gtgacattct gcctgaactt
     3781 gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga tgagtatttc
     3841 tggcgacaca tcctgcaccg gggacatcag caggtgctgt ttgtggtgac gcaggccgac
     3901 aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc cgcacaggca
     3961 cagaatattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt acatccggtt
     4021 gttgccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc gctaatgaca
     4081 gcacttcccg accatgccgc cagtcccctg atgacccgcc tgcaggacgg gctgcgcacg
     4141 gaatctgtcc ggggtcaggc ccgtgaacag tttaccggtg cggtggaccg gatatttgac
     4201 acggcggaga gcgtctgtgt tgcctctgtt gcccgtaagg ttctgcgcgc tgttcgtgac
     4261 accgtggtct ctgttgcccg cgcggtatgg aactggattt tcttctgaac ccggtggtga
     4321 tgcaccagac tcttgttaca gatttttaac cccatgaaaa cacaatgcaa acacagccat
     4381 acccgggttg tccccttgcg ttgtgtttct caagttaata gaatgcatcg atcgttacta
     4441 tcatatcgat cgctataaac gatcgtttgt ggtaaatgac ctcctccgtg gttgtcatga
     4501 aaatgcgtct caggaaggtt aaataatgca cgaaaagaac atcgccctgc tttgtgatga
     4561 agccgaccga cttttgcaac tgaacattaa tctgctccgg caaatggttg atgaaccgga
     4621 tgtgttatct gacagtaaga acgaaaacag actgcttttt gataaacaga aagcactgaa
     4681 aagaattgag gagctggagg gcgaacaaat caaaaccgcc cgcagggaga tggtgctggc
     4741 tgttgtcggc acgatgaaag caggtaaatc aactaccatc aacgccattg tggggcagga
     4801 aattctgcct aaccgtaacc gccccatgac ctctgtaccg acgctcatcc gccacgttcc
     4861 cggaaaaact gagccggtcc tccatctgga acatattcag cccgtccgca atttattaag
     4921 cacactgcaa caaaaactcg acaccccggc aggacagcaa gtcgcacaga ccctgcagca
     4981 aaccggggat acctgcgaac tgctggatat tctggcaaat gatggctggc tcaaaaatga
     5041 ataccacggg gaagacgaaa tctttaccgg actggcatcg ttaaacgatc tggttcgcct
     5101 tgctgcggca atggggagtg agtttccttt tgatgaatac gcagaagtgc agaaactgcc
     5161 ggtgatcgac gtggaattca gccatctggt ggggatggat gcatgccagg gaacactcac
     5221 actgctggat acccccggcc ctaatgaggc cggacaaccg cagatggaag tgatgatgcg
     5281 ggatcaactg cagaaagcct ctgcggtgct ggcggtgatg gattacaccc agatgaactc
     5341 aaaagcggat gaagacgtcc gtaaagagct taatgccatt gctgacgtat cagccggccg
     5401 cctgtttgtg ctggtcaata aatttgatga gaaagaccgc aatggcgatg gggcagatgc
     5461 cgtacgccag aaagttccgg caatgctgaa cagc
//
LOCUS       DBKELA010000176         5455 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.178, whole genome shotgun sequence.
ACCESSION   DBKELA010000176 DBKELA010000000
VERSION     DBKELA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.178"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="ACUQR0_003875"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="ACUQR0_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HHV9110714.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="ACUQR0_003876"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="ACUQR0_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HHV9110715.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="ACUQR0_003877"
     CDS             complement(1477..1881)
                     /locus_tag="ACUQR0_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HHV9110716.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="ACUQR0_003878"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="ACUQR0_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HHV9110717.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="ACUQR0_003879"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="ACUQR0_003879"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HHV9110718.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         1447 a         1339 c         1438 g         1231 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat atgcg
//
LOCUS       DBKELA010000177         5399 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.179, whole genome shotgun sequence.
ACCESSION   DBKELA010000177 DBKELA010000000
VERSION     DBKELA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.179"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..497
                     /locus_tag="ACUQR0_003880"
     CDS             <1..497
                     /locus_tag="ACUQR0_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HHV9110719.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACUQR0_003881"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACUQR0_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACUQR0_003882"
     CDS             2500..3114
                     /locus_tag="ACUQR0_003882"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HHV9110720.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACUQR0_003883"
     CDS             3114..3443
                     /locus_tag="ACUQR0_003883"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HHV9110721.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACUQR0_003884"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACUQR0_003884"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HHV9110722.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="ACUQR0_003885"
     CDS             4530..4841
                     /locus_tag="ACUQR0_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HHV9110723.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4825..5374
                     /locus_tag="ACUQR0_003886"
                     /pseudo
     CDS             4825..5374
                     /locus_tag="ACUQR0_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1180 a         1342 c         1424 g         1453 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaatac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtgacgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcaaa cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tctggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggca
//
LOCUS       DBKELA010000178         5398 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.180, whole genome shotgun sequence.
ACCESSION   DBKELA010000178 DBKELA010000000
VERSION     DBKELA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.180"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..945
                     /gene="tauA"
                     /locus_tag="ACUQR0_003887"
     CDS             <1..945
                     /gene="tauA"
                     /locus_tag="ACUQR0_003887"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9110724.1"
                     /translation="NTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESG
                     ASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEAL
                     VVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQ
                     RGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAF
                     AKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAEL
                     TGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            958..1725
                     /gene="tauB"
                     /locus_tag="ACUQR0_003888"
     CDS             958..1725
                     /gene="tauB"
                     /locus_tag="ACUQR0_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HHV9110725.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1722..2549
                     /gene="tauC"
                     /locus_tag="ACUQR0_003889"
     CDS             1722..2549
                     /gene="tauC"
                     /locus_tag="ACUQR0_003889"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HHV9110726.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2546..3397
                     /gene="tauD"
                     /locus_tag="ACUQR0_003890"
     CDS             2546..3397
                     /gene="tauD"
                     /locus_tag="ACUQR0_003890"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HHV9110727.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="ACUQR0_003891"
     CDS             complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="ACUQR0_003891"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HHV9110728.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5001..5369
                     /locus_tag="ACUQR0_003892"
                     /pseudo
     CDS             5001..5369
                     /locus_tag="ACUQR0_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1256 a         1362 c         1491 g         1289 t
ORIGIN      
        1 aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc
       61 gtggcgtatc aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct
      121 aaagagagcg gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg
      181 gcgctggctt ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca
      241 gccagccaac aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa
      301 gcgctggtgg taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc
      361 gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt
      421 aaaccagggc tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag
      481 cggggtgata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac
      541 ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc
      601 tgggtggtgc gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa
      661 agcgccatcg atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg
      721 gaaaacatca gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg
      781 gtgaagggaa atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg
      841 aacaaagcaa tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta
      901 gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg
      961 ctgcaaatct ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac
     1021 ctgacgctgg aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc
     1081 accctgctga atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg
     1141 ggtaagcgta ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg
     1201 ctaccgtggc gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag
     1261 aaaatgcagc gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca
     1321 gaaaaacgct atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc
     1381 gcgctggcgg cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc
     1441 ttcacccgcg accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag
     1501 gtgctgttga ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg
     1561 ctttcatccg gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt
     1621 gttgcgggag agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa
     1681 tatgttttaa gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg
     1741 aaaaactgca ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa
     1801 gcattggcac gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga
     1861 ttagcccgct gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg
     1921 gaccgcaggg atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca
     1981 ttgtgctggc gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc
     2041 taagcccgac ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac
     2101 cgctggctta tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct
     2161 tactgatcta tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa
     2221 gcgcgcagca ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc
     2281 tgtggtttgt cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc
     2341 tgggtgtggg ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag
     2401 gatttatggt tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg
     2461 cggtgattgc gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc
     2521 tgacgccctg gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc
     2581 gtatatcggc gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt
     2641 tgaacagctt taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat
     2701 tacgccgcag cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt
     2761 ttaccctcat gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc
     2821 gccggataac gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc
     2881 gattctggca gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat
     2941 tgcggcctat gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga
     3001 gcatgatttc cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca
     3061 acgctggcgc gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca
     3121 tccggtgagc ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga
     3181 tgtcagcgag aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc
     3241 ggagtttcag gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt
     3301 gactcagcac tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac
     3361 gatccttggg gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg
     3421 cccccttggg gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc
     3481 cccctttctg agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa
     3541 agtagctgaa aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga
     3601 ctttctcttc atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc
     3661 tcacctgata cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga
     3721 ggtacgctcc agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg
     3781 actcacgaat cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt
     3841 ttaatgcgct tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg
     3901 aatacgacat aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct
     3961 gtacctggcc gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa
     4021 ccacggcttg cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca
     4081 gcacaccgca gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca
     4141 tttctggcac agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc
     4201 aggcgtcgct gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga
     4261 taccggcgtt ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca
     4321 cgcctggcat ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca
     4381 acaccaggtc gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt
     4441 tgcgcaggcg acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat
     4501 taaggtgcac agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt
     4561 gacaagaaga gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc
     4621 atattcactg aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt
     4681 ttggttgtgc tgctataaat tgaataattc aaactttgtt gcatattgat aagatattta
     4741 tctggtttgt gtttttgata tttataacgc gtacgcatca aaaataacat tatctgcaac
     4801 atactgaaat atcgtccttt tatcaatttg atgcgtgttt ttctccataa attatatatg
     4861 tccacattcg gacttagggg aaagaataat tgaaccattc gtctgtaacg cagcataatc
     4921 gttagcgcga aacataatat gttttctatg cattgataat tgatggatca acttattacg
     4981 tccctgagga gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag
     5041 cattggcttg cactctggct atcacctctc aggctaatgc agcgaccaac gatatttctg
     5101 gtcaaactta caatactttc catcactaca acgacgccac ctatgctgat gacgtttact
     5161 atgatggtta tgtaggctgg aacaactatg ccgctgatag ctattacaac ggcgatatct
     5221 acccggtcat taataacgct accgttaacg gcgtgatttc tacctactat ctggacgacg
     5281 gtatttctac caataccaac gccaatagtc tgacaatcaa aaacagcact attcacggta
     5341 tgatttattc cgagtgcatg acaactgatt agactggccc cctgaatctc cagacaac
//
LOCUS       DBKELA010000179         5350 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.181, whole genome shotgun sequence.
ACCESSION   DBKELA010000179 DBKELA010000000
VERSION     DBKELA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.181"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(75..557)
                     /locus_tag="ACUQR0_003893"
     CDS             complement(75..557)
                     /locus_tag="ACUQR0_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110729.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="ACUQR0_003894"
     CDS             complement(625..2574)
                     /locus_tag="ACUQR0_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HHV9110730.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="ACUQR0_003895"
     CDS             complement(2659..2994)
                     /locus_tag="ACUQR0_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HHV9110731.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="ACUQR0_003896"
     CDS             complement(2994..3350)
                     /locus_tag="ACUQR0_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HHV9110732.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="ACUQR0_003897"
     CDS             complement(3350..4846)
                     /locus_tag="ACUQR0_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HHV9110733.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="ACUQR0_003898"
     CDS             complement(4843..5004)
                     /locus_tag="ACUQR0_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HHV9110734.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="ACUQR0_003899"
     CDS             complement(5014..>5350)
                     /locus_tag="ACUQR0_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HHV9110735.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DBKELA010000180         5339 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.182, whole genome shotgun sequence.
ACCESSION   DBKELA010000180 DBKELA010000000
VERSION     DBKELA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.182"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACUQR0_003900"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACUQR0_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HHV9110736.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACUQR0_003901"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACUQR0_003901"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HHV9110737.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACUQR0_003902"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACUQR0_003902"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HHV9110738.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACUQR0_003903"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACUQR0_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HHV9110739.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1456 g         1283 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtt cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DBKELA010000181         5211 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.183, whole genome shotgun sequence.
ACCESSION   DBKELA010000181 DBKELA010000000
VERSION     DBKELA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.183"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..662)
                     /locus_tag="ACUQR0_003904"
     CDS             complement(<1..662)
                     /locus_tag="ACUQR0_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid family protein"
                     /protein_id="HHV9110740.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQS"
     gene            complement(674..1163)
                     /locus_tag="ACUQR0_003905"
                     /pseudo
     CDS             complement(674..1163)
                     /locus_tag="ACUQR0_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="structural cement protein Gp24"
     gene            complement(1167..2387)
                     /locus_tag="ACUQR0_003906"
     CDS             complement(1167..2387)
                     /locus_tag="ACUQR0_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HHV9110741.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2391..3134)
                     /locus_tag="ACUQR0_003907"
     CDS             complement(2391..3134)
                     /locus_tag="ACUQR0_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HHV9110742.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3025..4491)
                     /locus_tag="ACUQR0_003908"
     CDS             complement(3025..4491)
                     /locus_tag="ACUQR0_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HHV9110743.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4491..5211)
                     /locus_tag="ACUQR0_003909"
                     /pseudo
     CDS             complement(4491..5211)
                     /locus_tag="ACUQR0_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1115 a         1477 c         1297 g         1322 t
ORIGIN      
        1 gactggttac ctgcagatga aacgatgatg ctggacaaca gagcaaactg ctcaggcggg
       61 atcagcaaat cacgcggaac cacagagtaa ccagaagcgg cccacgcatc agacagcacc
      121 tggttaatgc ttgcgcggat ttcgtccggt gttgaggttg cccacgtttt ggcagcgttg
      181 ttgacaggca cgccgtccag ggtaacaagg cctttcaggt ttaatgcgga atcgccaaca
      241 tatacctgtt catcgttatc catctgccat ttcagttgca taccgtcata cttctgcgta
      301 tcaatcgggc ggccgacctg ctgagcagcc tgcaattcta tgaccgtcca gccaagttcc
      361 atcccccaca ggttcagcgg gttaccggat ttgccgatat ccacgttcac gccagcaata
      421 gcggttgagt ctttgcctac ccagtttttt ccattcggat ttgcgccagt acccgcagcg
      481 gcgaagctgg tattcgtcca gctggaaatg tcatctgcga tggaggcgtc ttcacgcagt
      541 tgaatatcgc ggctccaggt gtaccccacc agtggcaggt tcagcgtctg gtcgagtctc
      601 tccagctccc cgatgagaaa ggcaccagag ctgtcaacgg ttgcctgatc aaaagtaatc
      661 attcgtctgt tccttaaatc ttccaggaaa tttctgcatt gccgtcagca tcaccggcac
      721 ctgtgaattc agcgttggtc agcaccacgt ttttgccact gactgacgtg gacatgaatc
      781 cacccagagg cactttgatg gattcatcag tggagatcgc cttttttgat agtgctggca
      841 tcaaaatcag aaccgagatt aacggtcacg tagccacgct tcatggcgtc gcccgggaag
      901 ttcttgcctg ttcccacctg gcgaaccatg tccggctgcg acgtggtcgg ataagggcgc
      961 acgtagatcc ccttcacctt gtctgcggta tcaccatctg ccagcggcac gaaaaaaccg
     1021 tcatcatcgt atttaccagc caggccatag gcagcgaagg cgttatcgga tttaaggacc
     1081 accggttcga cggttaagtc ctgcgggcga gagacagccc cggcaatgcc aacaggcatc
     1141 cggtacagaa atacattatt cattttttac cctttacggt ttgcccagaa ttcagcgttt
     1201 tgtttgttca gggaagcgat actggtcatg cccatgttta ggcgctgtgc atcgccggtg
     1261 gtggcgcggg tgtttcgccc tttggcaacc tcagacacgg cattaaacgc catgtcgacc
     1321 gattgtttcg gtaatttgcg gatatccgca tcaccgacta tctggcgaac cagcgttttg
     1381 tcagcagaag ccagaacctc gcgtttgaac gcggtcggtt tcatcttacg gctcagatcg
     1441 atacccggaa cgataacttc ggcacgccag gctgagtcac cagtaatcgt ggtttcctct
     1501 tcatcgtcct cgccgtcacc ggtcggatta tcgtcaggct tattatcgtt atcgcccgtg
     1561 gcatttcttt ccagcttagc cagcagggct ttcagtaatg ttttgaggtc atcatcactg
     1621 tcgccggttg gatctccgcc catctctggt gctttgtccg gtagtggttg ctgcggggac
     1681 aggttgatgt tgagattaac gccctgcggc aaatccccct catctcctgt aaccgatgcg
     1741 ggagccgact ccaccagttc gttcatggtg tcggcatctc ctgtcttgat ggccgcacgc
     1801 atgcggttcc accagttttt cttttgattt gccattgtgt ctctgtctcc aattgcacaa
     1861 cgatttccgg ctctgccttt agggacaaga gccacatggt ttccggtaat atcgacctgc
     1921 tcggctttac atggctcggt ctgctcgtac tccgcgtcat agccgcacga cacttcgcgc
     1981 aggccatctt cgataagctg aatggcgctt tcgtctttga cgataaggtc agccagcatc
     2041 aaatcagact gctcacccgt cccgcgccgg acattctgga ggtgcccgac agcaagcact
     2101 ttccagttct cgggatttac cagccgcaca ttcccgtttt catcttcagg atgcaggatc
     2161 gtgatgctca tcccttcgaa tgaggcaagc gtggccggat ggaatacctg ctcaggagaa
     2221 cgcgtgacga ctatttcaac gaacttatcg ggtttcagtt ttggcaggtc atcagcacca
     2281 tagagctgct tacctgttcg tcctatcggc acgtctttgc acagcaacga gccgtcagcc
     2341 aggcggtatc gggtttcccc gagcctggtc tcaaaaaagt atttcatggg ttatgctgcc
     2401 cttttcattg cgaatggaac gcgaacaaac acgatctctt tatagcagcg gcagtttggc
     2461 agctcgcccg cgtggccggt catgccgtcc aaagttggag gtgaatccca gcggacgaat
     2521 ttacctttca tgtgatcgtg ggaatctcgg acatcgccat catcggctgt acgccaaata
     2581 taaccatccg agccaatagc tatcgctcgg gccatatcca gcgcgcccgt tgctcttccc
     2641 agttccgttc gggcaatcag atcggcccta gacttagcaa catcgccggt agatgcaatc
     2701 tctttagcaa actgctccgc ccgaccaccg gtgacaacag cctctatcgc tttgttctgg
     2761 atgtcgtaca ccctgtctgc ggcctcgaga ggcagagatt tgatgtactt gacctgttcg
     2821 gcgatgatgg attgcatcac ctggcccaca ggagcgcttt ccacaagatt gcggagctcg
     2881 cgactgatgt tcttgctgtg ttgccgccaa actttctcgt tctgccgggt taggtccgca
     2941 gtaaagtttt ccgcgacctt tgtcgcccag ggggtgatga tttcactgta acgctccagc
     3001 gccgcaataa tttccgtgat actgtcattt gaaccatcgt agcgaccatt tacgatgtct
     3061 ccgaccgccc tcgctatcct gcgtaggctg gttcgatacc ggatttccgc ctgacggctc
     3121 ctgcggttcg tcatcaggtt cgccgatgcc gggcggcgct tcatcttcgg cattctcgat
     3181 gtcctcgttg gtaatggatg cccctatgcc ggttacgtca gaattttctc gcaaatcagt
     3241 catagcggct ttcagtgtca tcagaccatc acccagcgcc gtactgattg cgttggtggt
     3301 gtttaacgcc accgttgagc gatcgacatc agacatttgc cagagcgggt taaactcaaa
     3361 cgtgaaatcg tccggcagcg gcttgccaag ttccgaacga tgcatgatgt ccagtatccg
     3421 ccgcaccgga agacgtaaac gcctctcctg caacgaactg atgcgatcgt aatagttggc
     3481 aaggtctgca tcaccggtag aaaatccttt cggggactgt ccgaacaacc gcaccagtgg
     3541 gataccaaca gcgccactaa tctgttctgc aaactgcgaa aggatgtcat ccagaccact
     3601 gaagctgtac tgatgggttt caaacttatc ccgcgagtcc atgagcgtca tgccttcatt
     3661 actctggaac tgtcgaatca ggtcgatatt cttcagcaac gcttcatacg caggaccacc
     3721 aagtgcgata agctcgcgta gcttctccac gctgtaggta cgcaaatgcg ccttgtagac
     3781 cagctgcgcc gcgccgacag tagcgctgtc gaacgcggta agacgatccc agatacgctc
     3841 tacaaccgac attccccatt cgttctcggt catcttctgc tgaaatggca acgtgacgcc
     3901 atcaaagcga atcaggcgac tgtgatgaat gcgccaggca ggaattcccg ttgctgtggt
     3961 caccacatcg taaaactcag gtttacccag gtccggcccc atatctttaa tgcggcgggt
     4021 cagtgccggg tcgaccatcc agcggtcgag cgggagaatc cccttaaact tgcccttacc
     4081 gatggtttcg ggtcgcagcg gggtcattgg tgcctgcccc tcaatcatga tgaaacccac
     4141 cgcgccgccg tagaggcgcg accatttcag cacgtcattc agcgcatccc agatttgcaa
     4201 ctcatccagt tgtgattcga gaatgccacg atcttttgca tcaatttccg aagtgatgcg
     4261 aatgcctttg cgggtcatat catccgggat agcatcgact gcttcgccga tgatccagga
     4321 tgaacgatag gaccattcca ccagcatgcg gttacgactg gtgaaattag cccggtaggt
     4381 ggatgctgag tgctggttag gtgtctgcat ccctactcgg gcaataaaat tctcataacc
     4441 atcagctgtg gcctgcacag ttcgccgcag ggcttgtttg tttcgtgcca tcaggcctgt
     4501 ctccccagct gttcccagat atccagcgat gtatcgattg gcgcgaaggc cataatgaat
     4561 gcatcagcaa cgtttggtga cggtatctcg cgttttgcga ggtctttttt actttcgacc
     4621 atcacacgtc cgttacggtc gaaatcacga tgaggtgttg tcagttccag tttcagcttt
     4681 tcaagcaacg gacaacgaga atctatgctg atcagctcat ccacaggata ctgttctccg
     4741 ttgttaatgg cgttaaacgt atttctgaaa cggtcagcca ccatgcctga gccttaagat
     4801 ttgcgaaaaa gtctttgttg gggatgccgt tgtactcgtc atctggctca tgcacaccag
     4861 caccggcgtt aaacctctgg taattcacac gtcgcgcgta tgcgttctcg ctcttccggt
     4921 cagcgttaat ttcagagaat ttagcaccgg cagacgcacc aacaccgata gagtcgtaaa
     4981 caatatctgc ttcacgctcc agcgccgcct gataagtacg ctggcagctc ttcagtaatt
     5041 catcttcttt ggccttccat tcgtcggccc agaaaacaac ggatccgtga cggtaaacgt
     5101 tagcgcactt atctgtacca ctgtcagcca cgtcaaagcc aatacgcttt cttccactgg
     5161 gttcgaaatt taacgttttg tgcgcatcca ctgcggcttc tatccaagac a
//
LOCUS       DBKELA010000182         5163 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.184, whole genome shotgun sequence.
ACCESSION   DBKELA010000182 DBKELA010000000
VERSION     DBKELA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.184"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..221
                     /locus_tag="ACUQR0_003910"
     CDS             <1..221
                     /locus_tag="ACUQR0_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9110744.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="ACUQR0_003911"
     CDS             complement(393..722)
                     /locus_tag="ACUQR0_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HHV9110745.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="ACUQR0_003912"
     CDS             complement(727..912)
                     /locus_tag="ACUQR0_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HHV9110746.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="ACUQR0_003913"
     CDS             complement(909..3548)
                     /locus_tag="ACUQR0_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HHV9110747.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="ACUQR0_003914"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="ACUQR0_003914"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HHV9110748.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5163
                     /gene="hslJ"
                     /locus_tag="ACUQR0_003915"
     CDS             4856..>5163
                     /gene="hslJ"
                     /locus_tag="ACUQR0_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HHV9110749.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1305 a         1322 c         1205 g         1331 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcagaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taa
//
LOCUS       DBKELA010000183         5156 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.185, whole genome shotgun sequence.
ACCESSION   DBKELA010000183 DBKELA010000000
VERSION     DBKELA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.185"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            188..445
                     /locus_tag="ACUQR0_003916"
                     /pseudo
     CDS             188..445
                     /locus_tag="ACUQR0_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="ACUQR0_003917"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="ACUQR0_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HHV9110750.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="ACUQR0_003918"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="ACUQR0_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HHV9110751.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="ACUQR0_003919"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="ACUQR0_003919"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HHV9110752.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="ACUQR0_003920"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="ACUQR0_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3234..3429
                     /locus_tag="ACUQR0_003921"
                     /pseudo
     CDS             3234..3429
                     /locus_tag="ACUQR0_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3603..4418)
                     /locus_tag="ACUQR0_003922"
     CDS             complement(3603..4418)
                     /locus_tag="ACUQR0_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHV9110753.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5156
                     /locus_tag="ACUQR0_003923"
     CDS             4515..>5156
                     /locus_tag="ACUQR0_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHV9110754.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVH"
BASE COUNT         1376 a         1175 c         1226 g         1379 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gttcac
//
LOCUS       DBKELA010000184         5037 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.186, whole genome shotgun sequence.
ACCESSION   DBKELA010000184 DBKELA010000000
VERSION     DBKELA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.186"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(712..1128)
                     /locus_tag="ACUQR0_003924"
     CDS             complement(712..1128)
                     /locus_tag="ACUQR0_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110755.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1155..1295
                     /locus_tag="ACUQR0_003925"
                     /pseudo
     CDS             1155..1295
                     /locus_tag="ACUQR0_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1295..2335
                     /locus_tag="ACUQR0_003926"
     CDS             1295..2335
                     /locus_tag="ACUQR0_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9110756.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2363..2452)
                     /locus_tag="ACUQR0_003927"
     tRNA            complement(2363..2452)
                     /locus_tag="ACUQR0_003927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2416..2418),aa:Ser,seq:cga)
     gene            2546..3343
                     /gene="mtfA"
                     /locus_tag="ACUQR0_003928"
     CDS             2546..3343
                     /gene="mtfA"
                     /locus_tag="ACUQR0_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HHV9110757.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3444..3519
                     /locus_tag="ACUQR0_003929"
     tRNA            3444..3519
                     /locus_tag="ACUQR0_003929"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3477..3479,aa:Asn,seq:gtt)
     gene            3681..4943
                     /locus_tag="ACUQR0_003930"
                     /pseudo
     CDS             3681..4943
                     /locus_tag="ACUQR0_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1394 a         1184 c         1129 g         1330 t
ORIGIN      
        1 cactatgacg gttaaacaga tgacgataag ggcgattaac atcgcctttt gctgcttcat
       61 agcctgcttc tccttgcctt tcggcacgta agaggctaac ctacatgtgt tcagcatgga
      121 ttgagcctca gattaatgtt aagcgtcttg caggacgcgt aatgttaact ggggcttttc
      181 tctatctgcc ttttggtgtt catgcctgag acagatagcc tcaagcaccc gcagtcattc
      241 tacttaacta agatttccct gcaaaccgtt tttgtccggc acagtaaata tccaactaaa
      301 ccaatagcgt tcgctgtatt taccgccagt attcaatgca catgaccgcc atgaacaccc
      361 ctaaaaaaag ggcatttata tgtccaaaca ttaatatcaa aacatcaatt ttttccatat
      421 accttgctgt gaagatgatg ggcatacatg atgcgaacaa ccagaacgca acaaacaaaa
      481 actgcaatgc gtttttcatt attcccccta caatcaatgt gcaataacat ttaaacacac
      541 ctcaatttgg ccggacatat aaatatctaa accagaaaaa atcacttaca tagcgttaca
      601 aactctttag tctaaatact catcgtaaaa cattccccat acttatcagt ccgttccgcg
      661 ccaggtagct tattgcctta tctggcaacc tataatcagg tttccgcttt ttcagttggc
      721 tggtcgttta accgacatag ttaacccatt aatctggttg ccggatgctg gtggattttc
      781 gcgttttaat tgttcataaa agtgcacagc tttaaccagt tcttctgatg taaccgggac
      841 tggtggggca gtgaataagg cctgaatttc atagttcggc ctgtcgttac aatcctcttt
      901 tttcggtaca tatttccagt caccaaccca caactccccc tgagagtcca taacaccttt
      961 tttcacgtag cgatatcgcc acgctatcgg ctctgcttcc agcgatgccc gtgcaatttt
     1021 gaataactcg ccctctactc gcgccatccc tgaattgggg tggcatttcg taatcgctat
     1081 ttttaatttg gcttcttcga ttaattgttc ttttgttaat tcagtcattt tcattaccgc
     1141 cctggtaatg ctgccaccag cgaaaatggt taatggaaaa cgggcttccg ttctatacca
     1201 atcgtagtgg caaaccgatt gtcagccggg atctatttac ctgcaataaa actttaccac
     1261 cacgcgaggt agagccgaat tttggtgcga tctgatggga agacgaagga aaaatcctga
     1321 acacgaaaaa ttacctccaa atgtataccc aaataaatat agttatgtat ggaaaccaac
     1381 atccagagaa tctgtaacac taaccgccat caaggatggt ttagctgctt tatggaaaaa
     1441 gtatgaggaa actgtaaata atcgcgatcg tgcaatgaca ttcggtcgct tgtgggaaaa
     1501 attcctcgcc agcgcctatt acagtgacct cagtccaaga acacaaaaag attatctgca
     1561 acatcaaaaa aagttgcttg ccgtattcgg taaggtgccg gcagattcca taaaaccaga
     1621 acacatccgt cgatacatgg acaagagagg ggaacagagt aaaacgcaag ccaaccatga
     1681 aaaaagcagt atgtcccgcg tttacagttg ggggtatgag cgagggtacg tgaaggctaa
     1741 cccatgtgca ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa
     1801 agaataccag gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc
     1861 ctatctgtgt gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa
     1921 cgacgggatc tttatccagc aagggaaaac aggaaaaaaa cagataaaag catggagtcc
     1981 acgattacag gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag
     2041 caatcaatac ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag
     2101 aaatcgcgca ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc
     2161 gaaaggaatt tcagactatg aaggaagcag tcgggataag caacttttct ctggtcacaa
     2221 aaccgagggg caagtgctaa tctatgacag gaaggttaaa gtttcaccga cacttgatgt
     2281 cccgttacct gaaaatattc caagaaaata ttccaagtgt gatttttgtc actgacttaa
     2341 tgatgtataa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     2401 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     2461 ggttgtcatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     2521 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     2581 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     2641 agaacaggaa caaagcaaat tggtcgctct tgccgaacgt tttttacagc aaaaacggct
     2701 tgttccttta cagggctttg agctgaattc attaagaagc tgccggatag cacttctatt
     2761 ttgcctgccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     2821 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     2881 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     2941 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     3001 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattt cgttgcgtga
     3061 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     3121 attagttggt gagaatgcgg cgagcattga tgcttatgtt gccagtgatc ctgctgaatg
     3181 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     3241 ttcattgtgg caacgtttct gtcaatttta tcaacaagat cctttgcaga gactgcatca
     3301 cgctaatgat acagactcgt tttcagcgac gaatgttcat taattaacaa ctttgaagat
     3361 taattaacca attgaaatgg cttatgaaat ttagtgttga cagacaaggt accgctaagt
     3421 aatatgcgcc ccgttctcac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     3481 atccgtatgt cactggttcg agtccagtca gaggagccaa ttttctgttt tcatgcatcc
     3541 ttgcgaatct ttatctgatg ttgattcaac aggttagtgt gaaaactctt ctcggtgcat
     3601 tttgatttta ccctacgcat cgggaaaaat tggtggtcaa atctgggatc aggttagttc
     3661 gataatggag tgacccccat atatccctta ccgacgcaaa aatccgcacc ctcaagcctt
     3721 ctgataaacc ctttaaagtc tccgattctc acggtctgta tctgctggtc aagccgggtg
     3781 gctcccgcca ctggtatctc aaataccgta ttagcggtaa agaatcccgc attgcgctgg
     3841 gtgcctatcc agccatctcc ctgtctgatg cgcgacagca acgtgaaggt atccgtaaaa
     3901 tgctggcgct gaatatcaac ccggtacagc agcgggctgc tgaacgtggc tcacgaacac
     3961 cggagaaagt ttttaaaaac atggcgctgg cgtgacataa aagtaacagg aaatggtcgc
     4021 agaacaccgc cgaccgtctg cttgccagcc tgaacaatca catctttccg gtcatcggga
     4081 acctacctgt atcagaactt aaaccccgtc atttcattga cctgctgaaa gggatcgagg
     4141 aaaaaggtct gctggaggtt gcgtcccgca cacggcagca cctgagtaac ataatgcgcc
     4201 atgcggtcca tcaggagtta atcgatacga accctgcagc aaaccttggc ggcgtgacca
     4261 cacctcctgt cagacggcac tatcctgccc tgccgctgga gcggctgcct gaactgcttg
     4321 aacgtattgg ggcatatcat cagggccgtg aactgacccg gcatgccgtt ctgctgatgc
     4381 tgcatgtgtt cattcgctcc agtgaactgc gtttcgcccg ctggtcagag attgatttca
     4441 caaaccgagt ctggacgata cccgcgacgc gagaacccat tattggcgtg cgttattccg
     4501 gccgcggggc aaaaatgcga atgccgcata tcgtccccct ctcagaacag tccatcgcca
     4561 ttctgaaaca gattaaggat atcaccggta ataatgaact gatcttcccc ggcgaccata
     4621 acccgtataa gccaatgtgt gaaaacacgg tcaataaggc actgcgggtg atgggttacg
     4681 acacgaaaaa ggatatctgc ggtcacggct tccgggcaat ggcatgcagt gcgctgatgg
     4741 aatcgggttt atgggcaaag gacgcagtag aacgccagat gagtcatcag gagcgcaata
     4801 ccgtgcgcat ggcttatatt cataaggcag agcacctgga agcccgcaaa gcgatgatgc
     4861 agtggtggtc ggattatctg gaagcatgcc gagaatctta tgcaccgcct tatacaattg
     4921 gtaaaaataa gtttatccca tagtatacta acgaatccca cactctggca aattaccacc
     4981 acctctacag gaggtggttc tatatagact tttatcaact tcatgacgaa aaacacc
//
LOCUS       DBKELA010000185         5023 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.187, whole genome shotgun sequence.
ACCESSION   DBKELA010000185 DBKELA010000000
VERSION     DBKELA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.187"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="ACUQR0_003931"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="ACUQR0_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HHV9110758.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="ACUQR0_003932"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="ACUQR0_003932"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HHV9110759.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="ACUQR0_003933"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="ACUQR0_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HHV9110760.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="ACUQR0_003934"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="ACUQR0_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HHV9110761.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGIMLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5023
                     /gene="yidZ"
                     /locus_tag="ACUQR0_003935"
     CDS             4377..>5023
                     /gene="yidZ"
                     /locus_tag="ACUQR0_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HHV9110762.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1208 c         1255 g         1415 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc atgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcgacac ctg
//
LOCUS       DBKELA010000186         4973 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.188, whole genome shotgun sequence.
ACCESSION   DBKELA010000186 DBKELA010000000
VERSION     DBKELA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.188"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACUQR0_003936"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACUQR0_003936"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HHV9110763.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACUQR0_003937"
     CDS             1020..1703
                     /locus_tag="ACUQR0_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HHV9110764.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACUQR0_003938"
     CDS             1700..2161
                     /locus_tag="ACUQR0_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HHV9110765.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACUQR0_003939"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACUQR0_003939"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HHV9110766.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACUQR0_003940"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACUQR0_003940"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HHV9110767.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACUQR0_003941"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACUQR0_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HHV9110768.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACUQR0_003942"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACUQR0_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HHV9110769.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACUQR0_003943"
     CDS             complement(4903..>4973)
                     /locus_tag="ACUQR0_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110770.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1175 a         1303 c         1240 g         1255 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DBKELA010000187         4919 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.189, whole genome shotgun sequence.
ACCESSION   DBKELA010000187 DBKELA010000000
VERSION     DBKELA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.189"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..161
                     /locus_tag="ACUQR0_003944"
     CDS             <1..161
                     /locus_tag="ACUQR0_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HHV9110771.1"
                     /translation="VKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLN
                     SVRNFIVE"
     gene            complement(362..1219)
                     /gene="sitD"
                     /locus_tag="ACUQR0_003945"
     CDS             complement(362..1219)
                     /gene="sitD"
                     /locus_tag="ACUQR0_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HHV9110772.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1216..2073)
                     /gene="sitC"
                     /locus_tag="ACUQR0_003946"
     CDS             complement(1216..2073)
                     /gene="sitC"
                     /locus_tag="ACUQR0_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HHV9110773.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2070..2897)
                     /gene="sitB"
                     /locus_tag="ACUQR0_003947"
     CDS             complement(2070..2897)
                     /gene="sitB"
                     /locus_tag="ACUQR0_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HHV9110774.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2897..3811)
                     /gene="sitA"
                     /locus_tag="ACUQR0_003948"
     CDS             complement(2897..3811)
                     /gene="sitA"
                     /locus_tag="ACUQR0_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HHV9110775.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4284..>4919)
                     /locus_tag="ACUQR0_003949"
     CDS             complement(4284..>4919)
                     /locus_tag="ACUQR0_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HHV9110776.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1311 a         1196 c         1176 g         1236 t
ORIGIN      
        1 aggtgaaact gtcgatgccg gaactggaag aactggcggc agcaatggtg caggcgcagg
       61 tcgatcgcaa cgatgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc
      121 tgaaggattt gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg
      181 tatgcaggaa cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt
      241 tctaggcgtt atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg
      301 accttccatg gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt
      361 gctaattggg tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc
      421 tggtaacggt aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg
      481 gtgcgctgtc gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc
      541 cagtcgccag taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag
      601 gcgcaatgag cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg
      661 tcagggaaac catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct
      721 gcgcctgttg ataatcaaag ctgaacagca aaaaatcccg ccattttacg ctaataacaa
      781 gtgtaaccag cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca
      841 tgtcgccgaa aagaatatgg tcaagatgta cgtctggttt aattgcaata tacaagatca
      901 gacctgcggc aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc
      961 tgttgtcttt caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg
     1021 tagccagagg taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca
     1081 tcgcgtcacc catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg
     1141 gtatggaaac taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct
     1201 ccagtaataa tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc
     1261 caaaagtcca tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac
     1321 cacgatgatt ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact
     1381 ggttacactg ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa
     1441 ccatgctgtt gcaccgggag tcaccaccag acagatcacc agaaacgcac caaccgtttg
     1501 cagcgccgca acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg
     1561 atgtaatcca atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca
     1621 tttgaaaaaa agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc
     1681 cggatcgatt gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt
     1741 caatgacacc ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc
     1801 atcttctttt aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc
     1861 ggaaaagaag gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg
     1921 gacaatagag tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca
     1981 tgacaggaaa gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata
     2041 ttcatagctg aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt
     2101 cgatgtgcca caaaagggcg ttcatcgtca gtaataatgc tttcttccga gccgttaagt
     2161 gtgacatggc gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg
     2221 tctgtcggcc cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc
     2281 gtgactgagc cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc
     2341 ctcaacaggc tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc
     2401 agcaggatca catcaccctg ctgtgcaatt gctctcgcca gaaagacgcg tttcttttgt
     2461 ccaccggaaa gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc
     2521 gcatcggtga caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca
     2581 tagcggccca tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct
     2641 gactgaggga cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga
     2701 acagatatct ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat
     2761 tttccggaac cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat
     2821 gcatcgcgca gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg
     2881 cctgcagatt gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac
     2941 tggtagtaac cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg
     3001 aatcgacata gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg
     3061 gtttatcgga aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat
     3121 caacaacctt acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat
     3181 aaagctcttt tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca
     3241 tccatcgctg attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg
     3301 taatcttggc tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt
     3361 attttatcaa cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc
     3421 atgcatgggg attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac
     3481 ccgaagagac aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca
     3541 attccagatt cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc
     3601 caggggtagg ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg
     3661 cagcatctcc agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct
     3721 tgaatttttc agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca
     3781 agagcatggt tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag
     3841 aacaaacata gcaacggcta tgttttatag cataacctat acaaaatgaa aataattatc
     3901 attaacagct ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt
     3961 gaggaaaaaa gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat
     4021 ggcaaaaatg gggcaaaacg ctgtataagg ggcaaaaaag gggcaaaaaa ggggcaaaaa
     4081 agagtgggtt atcgtagctt attgttgtcg ctgataatgt ttaacatatt gaaaaataaa
     4141 taaaatactt atgagtcaga tagttgtgat ttttgccctt acttgttcag gtcgtattgt
     4201 tctttctttg taatttgttg attttcttgc attatttcag ttctctggta ctaaatgggg
     4261 caaattgggg gcaaactttg caactacgat aaccgcgcat tcaacatggc tatctgttcg
     4321 tcgttcatgt catcaatcca cataccgtaa atttcataca ccatttgcgc agtttcatgc
     4381 cccatctggc tggctataaa tgccgggttc gctcctgccg tcaacagcca gcaggcaaaa
     4441 gtatgtcgcg tgtggtacgg attacggcgg cgaataccag cacgttttac tgctgcattc
     4501 catcttgtac ccaaactgct taccgagtaa taaggtttct gtttttcgtt acacaccctg
     4561 ggcatgaaaa caaaatgcag tttttgtttt tcagttctgc cgtactcccg atgataaaaa
     4621 gtgatttcgc ttttgcgatg atgcccggtc agtttgtatt gctccttcag tgcttcaaga
     4681 gccggctgca gtaatgttac cgtccggatc ccggcatttg tttttggggg accgaacata
     4741 tcaagtatcg tcaggtttct tctgacattc acaattccct tctcgaaatc cacatcctcc
     4801 cacgccagag ctgccagttc cccgtgacga agcccggagt aaacggcaaa tttccacaag
     4861 ttttggctct gtcctttttc actttccatt aatgcattga attctgtttt agataacgg
//
LOCUS       DBKELA010000188         4879 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.190, whole genome shotgun sequence.
ACCESSION   DBKELA010000188 DBKELA010000000
VERSION     DBKELA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.190"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..454
                     /locus_tag="ACUQR0_003950"
     CDS             <1..454
                     /locus_tag="ACUQR0_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9110777.1"
                     /translation="ISKLAALPGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPD
                     FPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKI
                     LATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            788..3067
                     /gene="ydeP"
                     /locus_tag="ACUQR0_003951"
     CDS             788..3067
                     /gene="ydeP"
                     /locus_tag="ACUQR0_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HHV9110778.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3315..3518
                     /gene="safA"
                     /locus_tag="ACUQR0_003952"
     CDS             3315..3518
                     /gene="safA"
                     /locus_tag="ACUQR0_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HHV9110779.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3586..4347
                     /gene="ydeO"
                     /locus_tag="ACUQR0_003953"
     CDS             3586..4347
                     /gene="ydeO"
                     /locus_tag="ACUQR0_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HHV9110780.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4749..>4879
                     /locus_tag="ACUQR0_003954"
     CDS             4749..>4879
                     /locus_tag="ACUQR0_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HHV9110781.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVA"
BASE COUNT         1397 a         1040 c         1084 g         1358 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacccggaaa tccgcgtaat tttacgtgga atattatttc
       61 aaataacagt gttgttatgc caacaggcgg ctgcaccgtt gatagtcgta atgtcaccgt
      121 ggatctcccg gactttcctg gaagcgcaga aatacccctt ggcgtatatt gctcaagcga
      181 acaaaagctc tcattttatc tttcgggtgc caccactgac agtagccgac aagtgtttgc
      241 taacacagca cctgatgcaa caaaagcatc gggagtgggt gtcactctaa tgcgtaatgg
      301 aaaaatactg gctacgggag aaaatgtctc cctggggaca gtaaataaaa gcaaagtacc
      361 tctgggttta agcgcaacct atggacaaac aggaaacaag gtttcagccg gaaccgtcca
      421 gtcagtcatt ggtgtcactt ttatctatga ataattgtga ttttgacctt agtctatgcg
      481 ctccattcac gaaaattgcg cggataatta ttttgtgaag gctattatcc tacacctgta
      541 agaaaatccg cgcacaattg aagccagagt cttatttttt atattgaaaa atatgttaat
      601 ttaaacacta tgagagatga tgcttaactt atcgtattta atctattgtt taacgataat
      661 agattaaaaa tagaaagaga gtagtccctt actcttatat atccatgttg gcgataatcc
      721 ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt ccacacagga
      781 taagtagatg aagaaaaaaa ttgaatccta ccagggtgct gcaggtggtt ggggtgctgt
      841 taaatccgta gcgaatgcag tacgtaagca gatggatata cgccatgatg ttattgccat
      901 gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc cagatcctaa
      961 gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct gggaagtcac
     1021 ggataagcag gtaaatgcct ctttctttgc tgagaatacg gttcaatcat tacttacctg
     1081 gggagatcac gagcttgagg ctgcggggcg tctcactcag cctttgaaat atgatgccgt
     1141 cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg gcgcacgcct
     1201 tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca cttccaatga
     1261 agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact ttcccgactg
     1321 ctccaacatg tgccatgaac cgacaagcgt gggtttggca gcgagtatcg gtgtaggtaa
     1381 agggaccgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca ttgggcataa
     1441 ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga aacggggagc
     1501 gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta ccgcaccgca
     1561 aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct actacaacgt
     1621 gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa ttgagcgcga
     1681 tgatgctgca agtgccgcag gtcggccctc attgcttgat gacgaattta ttcaaacgca
     1741 taccgtcggc tttgacgagc tacgccgtga cgttctcaat tccgagtgga aagatatcga
     1801 acgtatttct ggactaagtc agacacaaat cgccgaactg gctgacgcat atgccgctgc
     1861 cgaacgcacc attatctgtt acggaatggg gatcactcag cacgaacatg gtacccagaa
     1921 cgtgcagcaa ctggtcaatt tgctgttgat gaaaggtaac attggcaagc ctggtgcggg
     1981 tatctgccca ctacgtggtc actctaatgt acagggcgac cgaaccgtcg gtatcaccga
     2041 gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc tatggcttcc ccccacctca
     2101 tgcacctgga catgctgcaa ttgccagcat gcaagcaata tgtacggggc aggctcgagc
     2161 attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag cgagcgctgt
     2221 accgttaacg caattagatt tggcggtaca cgtagccact aagcttaacc gctctcatct
     2281 gctgaccgca cggcatagct atattctgcc ggttctgggg cgtagcgaga ttgatatgca
     2341 aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc atgcctcgcg
     2401 tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg tcgcgggaat
     2461 cgcgcaggca gcactacccc agagcgtggt agcctgggag tatctggtgg aagattatga
     2521 tcgcattcgc aatgacattg aagctgtgct gccagagttc gccgactata accagcgcat
     2581 ccgtcatccc ggtggttttc acctgataaa tgcagctgct gaaaggcgct ggatgacgcc
     2641 gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct cttcagcgtt
     2701 taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca cgacgattta
     2761 tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct ttatgagtgc
     2821 taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta ttgcgcttac
     2881 gccagacggt aagcgcagct cacgccgcat ggatagatta aaagtggtca tttaccctat
     2941 ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa cacttgataa
     3001 ccacgatcca ttaagtggca ttcctggcta taaaagtatt ccggttgaat tagaaccatc
     3061 aaattaatgc ctcttctcat ttcttctgct gtcatccgca cagcagaaga attcctcatt
     3121 gactattatt gcgcaatttg ctcacatgga ttaaattaaa ctacatacta taagatataa
     3181 acttctgcct acagctgtaa gaaactccgc tcagtactga agcaccagtc ctatttcttc
     3241 ttttctccag cctgttatat taagcatact gattaacgac ttttaacgtt atccgctaaa
     3301 taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa aatcacgcaa agagataact
     3361 ataaagaaat catgtctgca attgtggttg tcttattact gacacttacg ttgatagcca
     3421 ttttttcggc aattgatcag ctgagtattt cagaaatggg tcgcattgca agagatctta
     3481 cacatttcat tatcaatagt ttgcaagact ggaaataaac agcaaattat aaatgtgaaa
     3541 tgtttaaaag gtatcgataa aactttattg ttttaaggag ataaaatgtc gctcgtttgt
     3601 tctgttatat ttattcatca tgctttcaac gctaacattt tagataaaga ttacgccttc
     3661 tccgacggcg agatcctgat ggtagataac gctgttcgta cacattttga accttatgag
     3721 cggcatttta aagaaatcgg atttaatgaa aataccatta aaaaatatct acaatgcact
     3781 aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg
     3841 actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat
     3901 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa aggtttcatt
     3961 acactattaa ctaacggtgt gctatccgtt tccgggaaag tgagaaatat tgtcaacatg
     4021 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc
     4081 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct attagatgcc
     4141 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
     4201 caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
     4261 agtccgaaga gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaatacgggc
     4321 aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgct
     4381 acgtaagaat tatatggggc agccattccc tgccccgcgt tgtttttagg cgatatattt
     4441 attgaaataa ataagtgaca tccatcacat atttatgcac ttacataacc tgttgcatga
     4501 ttatttatga tctcaattcg gcattttgtc agtaaaatgc aataatttat taaatatcaa
     4561 taaattagtt gtttatcggc gagaaattac ttaatagaac agaaagtaat gtcaacgctt
     4621 tatggactgt tatttccctt tttttagcta aatctgctat ctctttatgt gactaacttc
     4681 acttacatcc acttatttct cttcgtaaaa ttacttcgga attaagtaca ataagaagag
     4741 gaacatttat gaagtctgca ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac
     4801 tggcatctgg tatggctgca tttgctgctc atgcggcaga tgatgtaaag ctgaaagcaa
     4861 ccaaaacaaa cgttgcttt
//
LOCUS       DBKELA010000189         4857 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.191, whole genome shotgun sequence.
ACCESSION   DBKELA010000189 DBKELA010000000
VERSION     DBKELA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.191"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..970
                     /locus_tag="ACUQR0_003955"
     CDS             <1..970
                     /locus_tag="ACUQR0_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHV9110782.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1551..2774
                     /locus_tag="ACUQR0_003956"
     CDS             1551..2774
                     /locus_tag="ACUQR0_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110783.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3021..3587
                     /gene="slp"
                     /locus_tag="ACUQR0_003957"
     CDS             3021..3587
                     /gene="slp"
                     /locus_tag="ACUQR0_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HHV9110784.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3743..4273
                     /locus_tag="ACUQR0_003958"
     CDS             3743..4273
                     /locus_tag="ACUQR0_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9110785.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4315..4857)
                     /locus_tag="ACUQR0_003959"
                     /pseudo
     CDS             complement(4315..4857)
                     /locus_tag="ACUQR0_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1555 a          998 c          901 g         1403 t
ORIGIN      
        1 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
       61 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
      121 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
      181 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt ttggtccgac
      241 tctcactttt ttatttgttt caccattact taatcccatt atcgtcggac taatgtggat
      301 gacctttggt tggaaagtca ccttgttgta cgcgattatc gcagccggcg tctccgttct
      361 tgccagtatt atcctggatt ctctggggtt tgaacgtcat atcattgcca gtaaaagctc
      421 atcagcaaat tgttgtgctc cagcaaaaac ttcgccggga acgacatata cgcccataaa
      481 agtgagttgt tgtagtccag cggctaaagc cattgagaac cccgtagtta cctgttgcag
      541 taccaaagct gtagtaagta ttaatcccat aaaactagcc accaaagatg cgttgcaaca
      601 gtttaaagat gtactgccat atcttttgtt aggagtatta ataggatctt ttatttatgg
      661 ctttattccc tcagcgtgga ttgccgctca tgcaggagca gataatccct tcgccatccc
      721 attaagcgcc gttgtcggta ttccgctgta tatccgggct gaggcggtta tccctctggc
      781 atctgttctg atgacaaaag gaatgggtct gggagcatta atggcattaa tcatcggcag
      841 tgccggcgca agcctgacgg aagtgatatt acttaaatca atgttcagaa tgccgatgat
      901 agctgcattc ctgacggtta tattaggtat ggctatcttg atgggctatt tgactcaatt
      961 gctattttaa atagatgcca gcagtaaact ataagatgca tagttagtgc catatccttc
     1021 taagtagaaa atcatagaca ggataatcct taaaaatcac aaatgattta actaatgcat
     1081 tgagaatgat gacggctcgg tcaacaagtc atcaacagac aataaccacg taatcatgct
     1141 tctctgacat tttatcggag aagcaatgac tctttgtctc agtaccctcc tcctttttaa
     1201 aaggcagatt tcagtcgcta atacaacaca tttttaaata cactttaacc tggttcatta
     1261 ttacacatct aaaacatcac aaaaatatta cttcacacac tatcggagaa cataataact
     1321 ttttaaaatg agcgtttttt gatagttgtt aacgttttcc tttacgatac tatcactgcc
     1381 ctttttttat acacaaatat taccgggtgg aaatgaaagg gaatttaaag gtaattatat
     1441 caggaagata taacgcatta tatttattgt gtgtagaaga aaatccattc ctgttctaat
     1501 tatccaattt aaaaacatta gcataaaaca gatgatgaat aaggaatgtt atgtcaattg
     1561 actttacccc aggtataata aatacatatc acggcgatat ttataactgc acaacaaata
     1621 ccgataatgt taaaacgcca gacacaccga agtggccttg tgataattgg gaagaacaac
     1681 aacccattaa ttccacattc tctggagaag gatacaaccc tgaacaattt gatttagcgc
     1741 aacaacaact ccaacaaatt aatgcatgcc acacgaacac cacatataca aatgcagact
     1801 actcaaaagt tgtggttcaa cttgtaagtc ttattaataa cattgaaacg ataagttcga
     1861 caccacttac gcaacagaca caatcaatac ttaatcagat tcataacatt agatacgaaa
     1921 aggataagga ttctgtatgt caaatcattg ttgtcgttaa tccagaaccc aacaatccaa
     1981 tcataactaa aattctcgta gaagaaggaa tacccgaaag attcagtgtc caaaccgttt
     2041 cttccgataa taaaaatttt gctgggcaac gagctgactt accaactgat ataagagaca
     2101 ttcaaagctt gtacttaaaa atggcgaagc tgtatattga gcacagtaaa aatgaaaata
     2161 ggatggaagc ccttgctgga tgcgacttta ttgatttcaa catgacaggg caagatatgt
     2221 ccaagctagt cctaacctta tcaaaatttt attttgaaga tctacttaat ataaatttca
     2281 ctgacgcaaa tttatttaac actatttttt cacacaagga aaatcccatc cccaaactac
     2341 ataaatatga acaacatctt gacaaacaaa tcaatggttt gttttctaca ttgttaacta
     2401 ttaatgacaa ctctttacgc gcgaaagcgg aaattgcttc gagaataatt gatttcttag
     2461 aggcaaaggt agtgaatctt tcatttgatg acatattaaa atataagcaa gagttcaaaa
     2521 aaatatgtta taaacaactt caagagttta caacaccctc tctatacaat aaaattcaaa
     2581 aatgggcaac aatgtctaaa aatgaattca tagcatttca ttacgagaca cttcaacccg
     2641 aaaagatttc ataccccttt tatttaaaga gagatctgcc taatgaaaaa gatataaatt
     2701 atggggtgga aatagaaata ccatctggca aaagaattcg cttatcaaat cattatcaga
     2761 atataatacc ataatattga ttattagcac tttataatca ttgaataaaa atagatttta
     2821 tgtaatttta aaacaaaggg ttatattatt gggcgatgga tactcatacc acgccccaaa
     2881 ataacggaat tgaactaaac agaagggata taaacatcag acaggtttac gttactatca
     2941 ggcatatcac ctcagaatca gatgaaaact ataaagaaat atctattatg gttttaatat
     3001 ttgttgataa ggatagtaac atgaacatga caaaaggtgc actcatcctc agcctttcat
     3061 ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa aggcaataac caacctgata
     3121 ttcaaaaaag ttttgttgct gttcataacc agccggggtt atatgttggt caacaagcgc
     3181 gctttggtgg gaaggttatc aacgttatca atggcaaaac ggatacgttg ttagaaatcg
     3241 ctgtattacc gttggatagc tatgcgaagc ctgatattga agccaactat cagggccgac
     3301 tgctcgccag acaaagcggc ttccttgatc cagtgaacta tcgtaatcac tttgttacca
     3361 tcctcggtac cattcagggt gaacaaccag gctttatcaa taaagtcccg tataacttcc
     3421 tggaagtgaa tatgcagggc atccaggtgt ggcatttgag agaagtggtt aataccacct
     3481 ataacctgtg ggattacggc tatggtgcat tctggccgga accgggctgg ggtgcgcctt
     3541 actacaccaa tgcggtgagt caggtaacac ctgagctggt caaataacac caccgaaaga
     3601 tgcagatgct ttctcagcat ctgcatcatg tattacataa aattaatata cagtaagcta
     3661 actattatta ttataagccc tgtcctgtta attacctttg gcaaactgat tataaagtta
     3721 atgtccgcac caggagtcgg ttatgtttct tataattacc agggatacga tgttcttcac
     3781 cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat atacagaacg aagaagagat
     3841 cgacgtaatg ttgcatcaga atgccttcgt cattattgat acattaatga ataatgtatt
     3901 tcattctaat tttctcactc aaattgaacg attaaaacct gtccatgtca ttgttttctc
     3961 cccctttaat attaaacgct gtctggggaa agtaccggtg acctttgttc cgcggactat
     4021 cactatcatt gattttgtcg cactcatcaa tggcagttac tgctctgtgc ctgaagcgaa
     4081 tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc attgcgaatc aaatgacaac
     4141 tgaagatatt ctggagaaac tgaaaatatc gctaaaaacg ttctactgcc ataaacacaa
     4201 tatcatgatg atcctcaatc ttaagcggat caatgagctg gtacgccatc agcatattga
     4261 ttatctggtg tgaatttcag gcttacggtg agtctggcta cgctgccaca cagattagct
     4321 aattgaaacg cctttcaccc ctgccatacc ttttaataat cgcaacaggt cttctatcaa
     4381 cgttgtggca tgaagctgga tatcaatggc gaccacttca tgatcttcat tttcctgcaa
     4441 cgaaactaaa tcagttttta ttttttgctg cttaaaccag tcgagcattg ataccgtatt
     4501 accgttcact aatgtcagct gtaaatggta atttttgttc atcaggcgga aggttaattg
     4561 atggaagact tccagcacca acaaggtcat cactgaacca taaataccca gttcgtacat
     4621 accgctgcca ataaccatac ctatgcggcg gtcacccaga tatccgctgc cgtcgtcaga
     4681 cctacaatat tttggttacg aacgagaatg ttacctgcac cgataaaccc gacgcccgtc
     4741 accacctgag cagcaatacg gctggggtcg agtccgacgt gatctaaaga cagcacgtca
     4801 gcaaaaccat atttcgaaac aatcataaac agggcgcttc ccatgccaat taataca
//
LOCUS       DBKELA010000190         4840 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.192, whole genome shotgun sequence.
ACCESSION   DBKELA010000190 DBKELA010000000
VERSION     DBKELA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.192"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACUQR0_003960"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACUQR0_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HHV9110786.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACUQR0_003961"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACUQR0_003961"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HHV9110787.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="ACUQR0_003962"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="ACUQR0_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHV9110788.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="ACUQR0_003963"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="ACUQR0_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HHV9110789.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="ACUQR0_003964"
     CDS             4170..4343
                     /locus_tag="ACUQR0_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110790.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4369..4437
                     /gene="yncP"
                     /locus_tag="ACUQR0_003965"
     CDS             4369..4437
                     /gene="yncP"
                     /locus_tag="ACUQR0_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HHV9110791.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="ACUQR0_003966"
     CDS             complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="ACUQR0_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HHV9110792.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4665..>4840)
                     /locus_tag="ACUQR0_003967"
     CDS             complement(4665..>4840)
                     /locus_tag="ACUQR0_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHV9110793.1"
                     /translation="YWLSGDSGGQSNEFSNISELKDAVTEYIEYYNSRRISLKLKGLT
                     PNEYRNQTYMPRV"
BASE COUNT         1237 a         1117 c         1204 g         1282 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     4501 gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
     4561 tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa ttgcttggtt
     4621 cattcgttgg cctcggtttg tactgacccc aaaaagttgg acagttaaac acgaggcata
     4681 taggtctgat tccgatattc atttggagtc agacctttta atttcaggct aattcttctg
     4741 ctgttgtagt attcaatata ttccgtaaca gcatccttca gttcgcttat attactgaac
     4801 tcattagact ggccccctga atctccagac aaccagtatc
//
LOCUS       DBKELA010000191         4776 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.193, whole genome shotgun sequence.
ACCESSION   DBKELA010000191 DBKELA010000000
VERSION     DBKELA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.193"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..511)
                     /gene="coaBC"
                     /locus_tag="ACUQR0_003968"
     CDS             complement(<1..511)
                     /gene="coaBC"
                     /locus_tag="ACUQR0_003968"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HHV9110794.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPL"
     gene            683..1351
                     /gene="radC"
                     /locus_tag="ACUQR0_003969"
     CDS             683..1351
                     /gene="radC"
                     /locus_tag="ACUQR0_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HHV9110795.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            1568..1804
                     /gene="rpmB"
                     /locus_tag="ACUQR0_003970"
     CDS             1568..1804
                     /gene="rpmB"
                     /locus_tag="ACUQR0_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HHV9110796.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1825..1992
                     /gene="rpmG"
                     /locus_tag="ACUQR0_003971"
     CDS             1825..1992
                     /gene="rpmG"
                     /locus_tag="ACUQR0_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHV9110797.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            2090..2899
                     /gene="mutM"
                     /locus_tag="ACUQR0_003972"
     CDS             2090..2899
                     /gene="mutM"
                     /locus_tag="ACUQR0_003972"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HHV9110798.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2938..3417)
                     /gene="coaD"
                     /locus_tag="ACUQR0_003973"
     CDS             complement(2938..3417)
                     /gene="coaD"
                     /locus_tag="ACUQR0_003973"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HHV9110799.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(3425..4702)
                     /gene="waaA"
                     /locus_tag="ACUQR0_003974"
     CDS             complement(3425..4702)
                     /gene="waaA"
                     /locus_tag="ACUQR0_003974"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HHV9110800.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
BASE COUNT         1182 a         1181 c         1296 g         1117 t
ORIGIN      
        1 ttaacggatc gagcattcgt ccaggaccga tatcaccaca agcctgactg ccactgtctg
       61 gcccccagat gagcaaacca cgggaagcaa gcacctctaa attatgctgc gtggcagcgg
      121 cacggtacat ctgctggttc atggcgggga gcacggctac aggcgcaggt gtagccagac
      181 aaatcgtcga taccaggtca ttcgccattc cggcagcaac acgggcaatc aaatctgccg
      241 tggcaggggc gagaatcact aaatcagccc atttacccag ctcaatatgg cccatagcgg
      301 cttcggctgc cgggtcaagc agactgtcgg aaacgggata accagaaacc gcctgcaagc
      361 taagtggggt gataaaggct tttgccgctt cggtcatggc tacgcggacg tcggccccgc
      421 gatcgcgcaa acgacgcacc agttcagggg ttttataggc agcaataccg ccgctaacgc
      481 cgagaacgat ttttttaccg gccaggctca tgatgatttt tcctgttggg tgacaccaga
      541 agttggcgat tttatcacaa tacttttgtt gtcgtgcctt cacctgagat tcactttgcg
      601 aggctctttc caggattgaa aactggccgt cgatttaacg gaacggctat gacaggatgc
      661 gagcaccaca aaggaggtga aggtgaaaaa caatgcacag ctgttgatgc cgcgcgaaaa
      721 aatgctgaag tttggtatta gcgccttaac ggatgtcgag ctgctggcgc tatttctgcg
      781 taccggaacg cgcggtaaag atgtattaac cctggcaaaa gagatgctgg agaatttcgg
      841 ctctctttat ggcttgttaa cctctgaata tgaacaattt agtggtgttc atggaattgg
      901 cgtagcgaaa tttgcccagt taaaggggat tgctgaactg gcgcgacgtt actacaacgt
      961 gcggatgcgt gaagaaagcc ctttactcag cccggagatg acgcgggaat ttttacaaag
     1021 ccagctcacg ggtgaggagc gggagatctt tatggtgatc tttctcgact cacaacaccg
     1081 ggttataacg catagccgtc ttttttccgg cacgctaaac catgttgaag tccatcctcg
     1141 ggaaattatc cgcgaagcga taaaaataaa cgcctcggcg ctgatccttg cacataatca
     1201 cccttcgggt tgtgctgaac ccagtaaagc ggataaactc attactgaac ggataataaa
     1261 gagttgtcag ttcatggatt tacgcgtgct cgaccatatc gtgattgggc gtggagagta
     1321 tgtttctttt gccgaacgcg gctggattta acccgctatg cgcgatcctt cgggatcttt
     1381 gtctgttcgg gacttgagca catcgctgag tcagcgtata ctacgccacc tttgagaatc
     1441 tcgggtttgg catttgggcc tggcaatcga gagttcacat agaactgcga tgaccgggct
     1501 gtaaagcctg acgaggcgcc aataccccat acgaagctcg agctaatttg atttttggag
     1561 aatagacatg tcccgagtct gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg
     1621 ttcccacgca ctgaacgcga ctaaacgccg tttcctgccg aacctgcact ctcaccgttt
     1681 ctgggttgag agcgagaagc gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt
     1741 aatcgataaa aaaggcatcg atacagttct ggctgaactg cgtgcccgtg gcgaaaagta
     1801 ctaagtactt agaggaaata aatcatggct aaaggtattc gtgagaaaat caagctggtt
     1861 tcttctgctg gtactggtca cttctatacc actacgaaga acaaacgtac taagccggaa
     1921 aaactggaac tgaaaaaatt cgatccagtt gttcgtcagc acgtgatcta caaagaagcg
     1981 aaaatcaaat aattctcgct ttgatgtaac gaaaaacctc gctccggcgg ggttttttgt
     2041 tatctgcttg cccccatatt gattgcatct gttcattcct ggagatgcta tgcctgaatt
     2101 acccgaagtt gaaaccagcc gtcgcggcat agaaccgcat ctcgttggtg caaccattct
     2161 tcatgcggtg gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga tctaccgatt
     2221 aagcgatcaa ccagtgctta gcgtgcagcg gcgggctaaa tatctgctgc tggagctgcc
     2281 tgagggctgg attatcattc atttggggat gtctggcagc ctgcgcatcc ttccagaaga
     2341 acttccccct gaaaagcatg accatgtgga tttggtgatg agcaacggta aagtgctgcg
     2401 ctacaccgat ccgcgccgct ttggtgcctg gctgtggacc aaagagctgg aagggcataa
     2461 tgtgctggcc catcttggac cggagccgct tagcgacgat ttcaatggtg agtatctgca
     2521 tcagaagtgc gcgaagaaaa aaacggcgat taaaccgtgg ctgatggata acaagctggt
     2581 ggtaggggta gggaatatct atgccagcga atcactgttt gcggcgggga tccatccgga
     2641 tcggctggcg tcatcactgt cgctggcaga gtgtgaattg ttagctcggg tgattaaagc
     2701 ggtgttgctg cgttcgattg agcagggtgg tacaacgctg aaagattttc tgcaaagtga
     2761 tggtaaaccg ggctatttcg cgcaggaatt gcaggtttac gggcgaaaag gtgagccgtg
     2821 tcgggtgtgc ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa cgttttattg
     2881 tcggcagtgc cagaagtaat tcatgcgagc cggatggcat accatccggc ataaacgcta
     2941 cgctaatttt gccatcagcg cctgatggac attctccggc aggaaatggg tgacatcgcc
     3001 ctgatggcgc gccacctctt tcaccaacga tgaagagata aacgaccact ctttcgacgg
     3061 catcagaaac acactttcca gttccggcat taagtggcga ttcatatgcg ccagctgcat
     3121 ttcatattca aaatccgcca ccgcacgcag gccacgaatc agcaccgtag cgtgttgatt
     3181 acgggcgaag ttcgccatca aatcactaaa cccgaccact tccacgttcc ccagatgcgc
     3241 ggttgcctgc tgtgccagtg ccacacgctc ttccagggta aacatcggtt ttttactggg
     3301 gctggcggca atcgccagaa taacgtgatc gaacatctgc gtggcgcgcg tcacgatatc
     3361 gatatgacca ttggtaatgg gatcgaaagt acccggataa atcgcccgtt tttgcataac
     3421 aacctcaatg cgttttcggt ggcaggtaag gttccagcag ttgaagcaga cgctgtagcg
     3481 cgccctggtt ttgatacagt acttcaacgg catgacggcc atagaaacta cggtaaacgg
     3541 cgtcggtgag taaagaggaa acctcttttg caagcgtagt ggcatcggta acggtaataa
     3601 gcccgcttgc ctgctccagc cgcgcgcaaa tgtctttaaa gttaaaagta tgcggcccca
     3661 tcaataccgg aatagcgtgt gcggcagctt ccagcggatt atgcccacca cgttcaacca
     3721 gtgaaccgcc aacaaaggcg agatcggcaa tgccgtacag taacatcaac tcgcccatcg
     3781 tatcgccaac cacaacctgc gtgctggtgg aggggacttc ccctgaagag cgtgtgatat
     3841 agcttagtcc agcctggcgg acaaggttaa tcgcatccgg gaagcgttct ggatgacggg
     3901 gtaccaggat gagcaataaa ttcgggaatt gctgtaacaa tgcctgatgt gcggcaatga
     3961 ccacgctctc ttcaccttca tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc
     4021 attggcggcg tagcgtcacg gctttagcag ccaactgcgg agttacagaa atatcgaatt
     4081 tcaggctacc ggtaacggtt acctggttat tttttgcgcc cagcgccaca aaacgtgcac
     4141 catcttcttc attttgcgca gcaatcagcg taatacgacg cagcaagcgg cggacgaatt
     4201 tacccagttt ggcataacct gcggccgagc gggcagagag tcgcgcgtta gcgatcacca
     4261 gcggaatttt acgtttatgt agcgccgcaa tcaggttagg ccatagttcg gtttccataa
     4321 tcaacaccag tttggggtcg actttattca ggaaacggtt gagcgcatcg ggcaggtcat
     4381 acggcagata aacgtgctga acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg
     4441 ttggcgtcat ggttgtaacg gtaatcggta aatcaggata acgatgacgc agcgcgcgca
     4501 ccaacgggat cgccgccaga gtttcaccga cggagacgga gtgcagcata atgccgcctg
     4561 gttttagcgg atggcggtaa aaaccgtaac gttcacccca gcgttttcga taggccggag
     4621 ccttacgtcc gcgcacccag agccgtatcc agatcagcgg ctgaataagg tagagaaggg
     4681 cggtgtaaag caattcgagc atagtaaata gctgacttat ggatgtgctg gggattctat
     4741 gtatttagct gtggctttac cattactttt cccgtt
//
LOCUS       DBKELA010000192         4637 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.196, whole genome shotgun sequence.
ACCESSION   DBKELA010000192 DBKELA010000000
VERSION     DBKELA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.196"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..312
                     /locus_tag="ACUQR0_003975"
     CDS             <1..312
                     /locus_tag="ACUQR0_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HHV9110801.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            469..1218
                     /locus_tag="ACUQR0_003976"
     CDS             469..1218
                     /locus_tag="ACUQR0_003976"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HHV9110802.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1240..1806
                     /gene="yieF"
                     /locus_tag="ACUQR0_003977"
                     /gene_synonym="chrR"
     CDS             1240..1806
                     /gene="yieF"
                     /locus_tag="ACUQR0_003977"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HHV9110803.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="ACUQR0_003978"
     CDS             complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="ACUQR0_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HHV9110804.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3362..4018
                     /gene="yieH"
                     /locus_tag="ACUQR0_003979"
     CDS             3362..4018
                     /gene="yieH"
                     /locus_tag="ACUQR0_003979"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HHV9110805.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4085..4558
                     /locus_tag="ACUQR0_003980"
     CDS             4085..4558
                     /locus_tag="ACUQR0_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110806.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGMMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1138 g         1098 t
ORIGIN      
        1 gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
       61 gaattcgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
      121 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
      181 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
      241 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
      301 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
      361 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
      421 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
      481 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
      541 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
      601 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
      661 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
      721 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
      781 ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
      841 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
      901 attacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
      961 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     1021 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa
     1081 aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac
     1141 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     1201 tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg
     1261 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     1321 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
     1381 ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
     1441 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     1501 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
     1561 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     1621 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
     1681 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
     1741 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     1801 atctaaataa aaaacccgcc agtaatcatg catggcgggt ttttaatgcg ctatcgattt
     1861 taatgagcgt cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac
     1921 gggctaaggt cacgcagacg cccggtacca atcttcatca cgcagtagga gataaagccc
     1981 agcgcaatac cttcggtaat cgaaaagctg aacggcatca tcacggcggt aataaacgcc
     2041 ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg
     2101 ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca ccattcccgc cagcggcgac
     2161 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
     2221 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
     2281 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc
     2341 cccttctcat ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
     2401 aacaagttga ccaacatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
     2461 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
     2521 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
     2581 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga aaaagccgag gatacccaga
     2641 agtacgctgt gagaagtcag attaccgatg ctcatcagcg tttccgagtt agcgacaatc
     2701 acacctgcgt ttttcagccc catcatgccg ataaacaggc cgataccgct ggtaataccc
     2761 acacgcagac ttaccggaat gttagcaatc atccagtaac gaacgcggaa aatggtcaaa
     2821 agaagcagtc cgacagcgcc ccagaagatt gcgcccatac cgatctgcca cggcagcccc
     2881 attgcctgga caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc
     2941 accggaaggt ttgcaaacaa tcccatcata atgctaccga aagcagcaat cagacaggtt
     3001 gttacgaaga cggcactggt gtccatgcca gcaacgccca gaatttgtgg gttaacaaat
     3061 acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta
     3121 gtaccatgtt cgcgcagttt aaaaacacgt tccagcatcc tctgaccaga agtctgggtg
     3181 gtatgttgat gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
     3241 accaaaatgc gacaataggc gcgtttgtga gagacttttt tattggattt acttatacgg
     3301 caacgattgc gttgcgcaaa tcggctctac gaaaacgtta aactgattaa aaaggaaagg
     3361 catgtcccgg atagaagcgg tatttttcga ctgcgacggt acgctggtcg acagtgaagt
     3421 catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc tcgatcctga
     3481 agaggtattc aaacgtttca aaggtgtaaa actgtacgaa attatcgata ttgtttccct
     3541 tgaacatggt gttacgttag cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc
     3601 tcggctgttc gattcagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc
     3661 aatgtgtgtg gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa
     3721 tatgttgcac tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc
     3781 agacccggcg ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat
     3841 tctggttgat gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt
     3901 ctacttctgc gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac
     3961 ccatctttcg cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt
     4021 cttcacactc ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt
     4081 tgatatgtct gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc
     4141 gttaattggt gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct
     4201 tttggtaata atacaggtac ttccattgtt tctcttattg tcgattacta cgggagctat
     4261 tcctgcattg ttaaccggca tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa
     4321 aaattatcgt tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc
     4381 agttattgta catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg
     4441 tgacagtctc gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat
     4501 cattacccgt ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc
     4561 gggatacttt atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat
     4621 caggccgtta ccccaat
//
LOCUS       DBKELA010000193         4613 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.197, whole genome shotgun sequence.
ACCESSION   DBKELA010000193 DBKELA010000000
VERSION     DBKELA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.197"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..367)
                     /locus_tag="ACUQR0_003981"
     CDS             complement(<1..367)
                     /locus_tag="ACUQR0_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HHV9110807.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFE"
     gene            complement(406..693)
                     /locus_tag="ACUQR0_003982"
     CDS             complement(406..693)
                     /locus_tag="ACUQR0_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110808.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(943..1098)
                     /gene="hokD"
                     /locus_tag="ACUQR0_003983"
     CDS             complement(943..1098)
                     /gene="hokD"
                     /locus_tag="ACUQR0_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HHV9110809.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1326..1991)
                     /locus_tag="ACUQR0_003984"
     CDS             complement(1326..1991)
                     /locus_tag="ACUQR0_003984"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HHV9110810.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2160..2516)
                     /locus_tag="ACUQR0_003985"
     CDS             complement(2160..2516)
                     /locus_tag="ACUQR0_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110811.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(2574..2996)
                     /locus_tag="ACUQR0_003986"
     CDS             complement(2574..2996)
                     /locus_tag="ACUQR0_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HHV9110812.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            complement(3011..3757)
                     /locus_tag="ACUQR0_003987"
                     /pseudo
     CDS             complement(3011..3757)
                     /locus_tag="ACUQR0_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3764..4547)
                     /locus_tag="ACUQR0_003988"
                     /pseudo
     CDS             complement(3764..4547)
                     /locus_tag="ACUQR0_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1092 a         1257 c         1014 g         1250 t
ORIGIN      
        1 cctcaaacga ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat
       61 attgctctgc cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt
      121 aatcaacgaa accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      181 tccagtaaac gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
      241 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc
      301 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
      361 gtgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
      421 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt
      481 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
      541 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
      601 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
      661 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
      721 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
      781 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
      841 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
      901 agaggtgggc ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta
      961 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
     1021 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
     1081 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
     1141 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
     1201 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
     1261 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
     1321 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
     1381 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     1441 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     1501 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
     1561 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     1621 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
     1681 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
     1741 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
     1801 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     1861 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
     1921 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
     1981 gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa
     2041 cggggctatc ggaaacagat gtgtgactgc tccccgccct ttcgggcggt ctcctgatga
     2101 tttgagggtg cagaaatccc tccggttaag gattaaattt ttaacagtgc taaatttaat
     2161 tattcagttc tggattttgt cgccctgcgt atccgcgctt tcgcgttacg ctcaatctgt
     2221 atcagctttt ctatatttct ccgcctttcc tgttcctcct ggcgcaatag ccttacatca
     2281 tctgccagtc tggtttctct ttttgccaca gagagcatcc agtcaaacgg ctccacaacc
     2341 gcaccgcaga ttttacagcg gacctgacgc tctttttcgt caacccgtac agaggcgtga
     2401 tggcaatatg gtctttccga tggcttataa agaaaattaa cctgattacg agggtcatcc
     2461 tcttttatcg gaaataaaac gatattgctt aactcatctt ctggttttat ttccatgctc
     2521 ctctcctttg atgcgaatgc cagcggtaat tgaagcctga tagctaattt cattcacagt
     2581 atcgcctcct gaaaattacc ctgatagaaa gccagtacac gctgcatagc ttcgctcttc
     2641 cggcactcgc gacagattat attcaggcgc ctgtcgtagc ggcgtatttc tccgtcaggt
     2701 aatgaccaga taaggtccgg atcaacaaca accggtttcc tcacctttac tctcgagagt
     2761 tttttgcggg cgctttgcca gtccttgcga gcctgttcag acgggaataa gccgtagcca
     2821 gaattatata catcaccact tgcgaccagt tctatgcata agcgaccgac agacgcacga
     2881 ctgatacctg tttcatcgga caactgccga atcgtgaccc gaccgtcctg atgcacgagt
     2941 tccacaattc gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt cgccataaat
     3001 cctcctgaaa ctacttaaca accctcgaat ggctaacatt cggacgccag ctctcccagt
     3061 taaaattaac ccagcgcccg ccgttcatga ccatgcgatc catcacccgc tcgccgagca
     3121 atgttttcat cacctcatag ttcaggtttg tcagcatccc cacgctgcgc atcgacgctg
     3181 tccggcgatc aacaatctgg tgcagaacca cctgctcgtt tttcgtctcg cgctgaatgc
     3241 caatttcatc aagaaccagc agatccactt cgcacagctc ccgcaaaaat ttttcgcctg
     3301 actgcccatc gtcatagctg gcgtgcaggg cgctcataac atcagccacg gtaaccacaa
     3361 tcactgtctg accgtctttc agcaggcgat tcccgatagc tgccgctaag tgattctacc
     3421 cggtatcagg ttttccgctg aacgcaaaat ttgtacaccc ggtcatcagt tcatcggcga
     3481 tggatttcgc ctggcttaac gcgtatcgct ggccgtcgtt ctgcacctgg taattcgcaa
     3541 acgagcattt acggtgcaac ggctggatgc ctgagcgatt cagaattttt tccacccgca
     3601 actggcgatt caggcggttg atctcctcgc tacgtttctg gccttcagca agttgccact
     3661 cgcgccactc cgctaccgtt ctgaatgggg cggttacatg ttgcggggtc agtcggcgga
     3721 tgcgttccag aacgccgcct gtcgcaatat ttttcatggt ccgttacccc ctgaagcctg
     3781 gcgggatcgc actgtccggc aacgagacgg tgttaacctg tcggagcaac gtctcaggcc
     3841 gaacaccttt cggcgcgaac aggccctggt attcattggc gatgctgtgt cgaatcacct
     3901 gctcaggtgt aaaaccctgc tgacggaatt tttccagttc ccgtatcgcc ctgctccgtt
     3961 cgaatcggtt ttcgcaatgc ctgtctgaac cggacccact catgccagag tgtttccggc
     4021 aaccaatcgg gcagctcaat agcctccggc tcgaattttt tagacgctcg tttttggcga
     4081 gggggattta gggggagatc agtatttaga tcttcctctt cctcttcctc ttccactggt
     4141 aacgcttttt gatccgtttg tgtaacgctg gcagcgttac cttttcgttt cagttcgcgt
     4201 atttttgtta ctcgctcgtt tgtaaccgcc cgttttttag agctttttcc gttatgacgt
     4261 tcaaagttag gtagagaaag cccaccgtca ttttcgacca gccatccaac ctgaattaac
     4321 gcatcagcaa aaccagacat aaaagtgatg cgatctattg cactttttgt aacgccgcga
     4381 gcgttacaat ctgcattacc gtctatcatt tgttgatccg cccatgccca gaagcgaata
     4441 accttcccta atgcggcatc tggatcaata ttcagaatct cagcaagcct gaatatttcc
     4501 ggcttatccg gcgtaataac ctcgagcttt atccagtttg aagccatttg ttttcacctt
     4561 gtaacgctcg cagcgttaca tttaactgat accgaacaaa acaatccggc acg
//
LOCUS       DBKELA010000194         4602 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.198, whole genome shotgun sequence.
ACCESSION   DBKELA010000194 DBKELA010000000
VERSION     DBKELA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.198"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..218
                     /locus_tag="ACUQR0_003989"
     CDS             <1..218
                     /locus_tag="ACUQR0_003989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110813.1"
                     /translation="LCRIPEDPHHWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLI
                     TDYEVQKYLSFQLPVIRYRERFWQYME"
     gene            225..2648
                     /gene="pgaA"
                     /locus_tag="ACUQR0_003990"
                     /pseudo
     CDS             225..2648
                     /gene="pgaA"
                     /locus_tag="ACUQR0_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2657..4602
                     /gene="pgaB"
                     /locus_tag="ACUQR0_003991"
                     /pseudo
     CDS             2657..4602
                     /gene="pgaB"
                     /locus_tag="ACUQR0_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
BASE COUNT         1315 a         1031 c         1060 g         1196 t
ORIGIN      
        1 atttatgccg tattcctgaa gatcctcatc attggaatgg attttcgggc gagaaaagga
       61 ttttatatgg acactctgct catcatttct tcttctcatc atcaacaatt cacgtctctc
      121 ttccgcgttt aataacggat tatgaggtgc aaaaatatct ttcttttcag ttacctgtaa
      181 ttagatacag agagagattt tggcaataca tggagtaata caggatgtat tcaagtagca
      241 gaaaaaggtg cccgaaaacc aaatgggctt tgaaacttct tactgccgca tttttagcag
      301 cgagtcccgc ggcgaagagt gctgttaata acgcctatga tgcattgatt attgaagctc
      361 gcaagggtaa tactcagcca gctttgtcat ggtttgcact aaaatcagca ctcagcaata
      421 accaaattgc tgactggtta cagattgcct tatgggccgg gcaagataaa caggttatta
      481 ccgtttacaa ccgctaccgt catcagcaat taccagcgcg tggttatgca gctgtcgccg
      541 tcgcttatcg taacctgcaa caatggcaaa actcgcttac actgtggcaa aaggcgctct
      601 ctctggagcc gcaaaataag gattatcaac ggggacaaat tttaaccctg gcagatgcgg
      661 gtcactatga tactgcgctg gttaaactta agcagcttaa ctctggagca ccggacaaag
      721 ccaatttact cacagaagcc tatatctata aactggcggg gcgtcatcag gatgaattac
      781 gggcgatgac agagtcatta cctgaaaatg catctacgca acaatatccc acagaatacg
      841 tgcaggcatt acgtaataat caacttgctg ccgcgattga cgatgccaat ttaacgccag
      901 atattcgcgc tgatattcat gccgaactgg tcagactgtc gtttatgcct acgcgcagtg
      961 aaagtgaacg ttatgccatt gccaatcgcg ccctcgccca atacgctgca ttagaaattc
     1021 tgtggcacga taacccagac cgcactgccc agtaccagcg tattcaggtt gatcatcttg
     1081 gcgcgttatt aactcgcgat cgttataaag acgtcatttc tcactatcag cgattaaaaa
     1141 agacggggca aattattccg ccctaggggc aatattgggt tgcatcggct tatctcaaag
     1201 atcatcagcc gaaaaaagca cagtcaataa tgaccgagct cttttatcac aaggagacca
     1261 ttgccccgga tttatccgat gaagaacttg cggatctctt ttacagccac ctagagagtg
     1321 aaaattatcc gggcgcgcta actgtcaccc aacataccat taatacttcg ccgcctttcc
     1381 ttcggttaat gggcacgcct acgagcatcc cgaatgatac ctagttacag gggcattcgt
     1441 ttctctcaac cgtagcaaaa tatagtaatg atcttcctca ggctgaaatg acagccagag
     1501 agcttgctta taacgcacca ggaaatcagg gactgcgcat tgattacgcg agtgtgttac
     1561 aagcccgcgg ttggcctcgt gcagcagaaa atgaattaaa aaaagcagaa gtgatcgagc
     1621 cacgtaatat taatctggag gttgaacaag cctagacagc attaacgtta caagaatggc
     1681 agcaggcagc tgtcttaacg cacgatgttg tcgaacgtga accgcaagat cccggcgttg
     1741 tacgtttaaa acgtgcggtt gatgtacata atcttgcaga gcttcgtata gctggttcaa
     1801 caggaattga tgccgaaggc ccggatagtg gtaaacatga tgtcgactta accaccatcg
     1861 tttattcacc actgctgaag gataactggc gcggttttgc tggattcggt tatgccgatg
     1921 gacaatttag cgaaggaaaa gggattgttc gcgactggct ggcgggtgtt gagtggcggt
     1981 cacgtaatat ctggctcgag gcggagtacg ctgaacactt tttcaatcat gagcataaac
     2041 ccggcgcgcg cctgtctggc tggtatgatt ttaatgataa ctggcgtatt ggttcgcaac
     2101 tggaacgcct ctctcaccgc gttccattac gggcaatgaa aaatggtgtt acaggcaaca
     2161 gtgctcaggc ttatgttcgc tggtatcaaa atgagcggcg taagtacggt gtctcctggg
     2221 ctttcactga tttttccgac agtaaccagc gtcatgaagt ctcaattgag ggtcaggaac
     2281 gcatctggtc ttcaccatat ttgattgtcg atttcctacc cagtctgtat tacgaacaaa
     2341 atacagaaca cgatacccca tactacaacc ctataaaaac gttcgatatt gttccggcat
     2401 ttgaggcaag tcatttgtta tggcgaagct atgaaaatag ctgggagcaa atcttcagcg
     2461 caggtgttgg tgcctcctgg caaaaacatt atggcacgga tgtcgtcacc caactcggct
     2521 acgggcaacg cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa
     2581 aacgacctta tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca
     2641 gattttaagg ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat
     2701 aattatgctc accgcgtgca tcagccagtc aagaacatca tttataccgc cacaggatcg
     2761 cgaatcttta ctcgccgagc aaccgtggcc gcataatggt tttgtggcga tttcatggca
     2821 taacgttgaa gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg
     2881 tgaacaattt gcctggctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg
     2941 tgaagcacat cgaggaggaa aaccgctacc ggaaaaagca gtagtgctga cttttgatga
     3001 cggctaccag agtttttata cccgcgtctt cccaattctt caggccttcc agtggcctgc
     3061 tgtatgggcc cccgtcggca gttgggtcga tacgccagtg gatgaacaag taaaatttgg
     3121 cgatgagatg gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg
     3181 ttcccggctc gttgaggtcg cttctcatac atggaattct cactacggta ttcaggctaa
     3241 ttccaccggc agcttaatgc ctgtatatgt aaatcgtgca tattttactg accacgcacg
     3301 gtatgaaacc gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga
     3361 atacctgcgt acaaaggctg aggtaaatcc acacgttttt gtttggcctt atggcgaagc
     3421 gaatggcata gcgatagagg aattaaaaaa actcggttat gacatgtttt tcacccttga
     3481 atcaggtttg gcaaatgcgt cacaattgga ttccattccg cgagtattaa tcgccaataa
     3541 tccctcatta aaagagtttg cccagaaaat tattaccgta caggaaaaat caccacaacg
     3601 gataatgcat atcgatcttg attacgttta tgacgaaaac cgccagcaaa tggatcgcaa
     3661 tattgatgtg ctaattcagc gggtgaaaga tatgcaaata tcaaccgtgt atttgcaggc
     3721 atttgctgat cccgatggtg atgggctggt caaagaggtc tggtttccaa atcatttgct
     3781 accaatgaaa gcagatattt ttagtcgggt tgcctggcaa ttacgtaccc gctcaggtgt
     3841 aaacatctat gcgtggatgc cggtattaag ctgggattta gatcccacat taacgcgagt
     3901 aaaatactta ccaacagggg agaaaaaagc acaaattcat cctgaacaat atcaccgtct
     3961 ctctcctttc gatgacagag tcagagcaca agttggcatg ttatatgaag atcttgccgg
     4021 acatgctgct tttgatggca tattgttcca cgatgatgct ttgctttcag attatgaaga
     4081 tgccagtgca ccggctatca cggcttatca gcaagcaggc tttagcggga gtctgagcga
     4141 aattcgataa aacccggagc aatttaaaca gtgggcccgc tttaaaagtc gtgcgttaac
     4201 tgacttcact ttagaactta gtgcgcgcgt aaaagccatt cgcggtccac atattaaaac
     4261 tgcacgaaat atttttgcac ttccggtaat acaacctgaa agtgaagcct ggtttgcaca
     4321 gaattatgct gatttcctaa aaagctatga ctggaccgct attatggcta tgccttatat
     4381 ggaaggtgtc gcagaaaaat cggctgacca atggttaata caattgacca atcaaattaa
     4441 aaacatccct caggctaaag acaaatctat tttagaatta caggcacaaa actggcagaa
     4501 aaatggtcag catcaggcta ttccttcgca acaactcgct cactggatga gcctattaca
     4561 actgaatgga gtgaaaaact atggttatta tcccgacaat tt
//
LOCUS       DBKELA010000195         4556 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.199, whole genome shotgun sequence.
ACCESSION   DBKELA010000195 DBKELA010000000
VERSION     DBKELA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.199"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            363..1720
                     /locus_tag="ACUQR0_003992"
                     /pseudo
     CDS             363..1720
                     /locus_tag="ACUQR0_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1818..3427
                     /locus_tag="ACUQR0_003993"
                     /pseudo
     CDS             1818..3427
                     /locus_tag="ACUQR0_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3528..4019)
                     /locus_tag="ACUQR0_003994"
     CDS             complement(3528..4019)
                     /locus_tag="ACUQR0_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HHV9110814.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4024..4556)
                     /locus_tag="ACUQR0_003995"
                     /pseudo
     CDS             complement(4024..4556)
                     /locus_tag="ACUQR0_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         1398 a          728 c          901 g         1529 t
ORIGIN      
        1 cccaatgcct ttttcaaaat aaattaggat taaaataatt aaatcagtaa attccgatgc
       61 atgatttcgg atttttcgaa agtcctgatg aaaggctgcg ctacggcact cacggattta
      121 attgttattc ctaatgtatc tatgaattaa tgttttataa ggatttttta taaccattta
      181 taagtatttt caagcctggc ttgttgcaaa caatgtataa agcacttagg caataataat
      241 tacattcagc aactatcatc atcggtattg tttgtgggcg gaatttcata ttgacaacag
      301 tacagttctt atatctatta ataatagaaa gagatctaca acctacagat tggtgtagct
      361 ttatggaaaa agactatttg agaattagta gtactgtatt agtgagctta ttgtttgggc
      421 ttgctttggt tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac
      481 gatcaaccta tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt
      541 ttatgcagtt gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg
      601 cttttctgag ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg
      661 aggggcgttt gacaagcagt gttgtccaaa atgacattgc aatctattat ttgtttcgtc
      721 agatgagttt gtgcatatta atatttctgg cattgatgaa taaagttagt gaaaacacaa
      781 aacagcgcaa tttattttcc aaaaaaatga ctttgtgcat aagtttgttt ttgtttttgg
      841 ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt tacacattgc
      901 cgagttaacc aatgaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      961 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctatgatat
     1021 ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct ctctgttatt
     1081 tatgagcagg tatagcgtgt ccacttggta tattagtcgc actattgaag tagtcagtaa
     1141 gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag taacaaagaa
     1201 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
     1261 gacagttcaa tcagcatcag ccaaaaaaac gccttattgt gtcatgatta tggatatcga
     1321 ccacttcaaa gaagtcaacg acacctgagg gcatccggtt ggcgatcagg tgataaaaac
     1381 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgtcggcgg
     1441 cgaagagttt ggcgtcttgc tgacggacat cgatactgaa cgtgcgaaag ctttagcgga
     1501 aaggattcgg gaaaatgttg agcgtttaac tggcgataat cctgaatacg ctataccaca
     1561 aaaagtgacg attagtattg gcgctgttgt gactcaggaa aatgcgttaa acccgaatga
     1621 gatctatcga ctggctgata acgcactata cgaggcgaaa gagaccgggc gtaataaggt
     1681 ggttgtgagg gatgtggtga gtttttgtga gtcaccataa agcggcattt tagcgggtga
     1741 ttttggtaag cgctaaatag ctattcgcag acttgcgtaa caattttcag aaatatcata
     1801 gtactatatg atcttaaatg cttatcattg atcaatctgc tatcacccgc ttttactggc
     1861 aattgtacta taatttctta ggttgcatca ccctgatgcg tggggaaaca tgtatggtat
     1921 tcaacaaaaa aatgtttgta ctcattataa taccaggcat tctgggggtg ttactatcgt
     1981 ttgctatgtc aatatttcaa atgaatagag atacgacaat taccgccggt attctgttaa
     2041 aacagcttga taatgttacg caaattgtcc agcatacgac aaagcttacc tctattctgg
     2101 tgatgaagcc ctgcaaagac attcttgaac agttgattgc taacggagca ttgactccct
     2161 atgttcgaac aacgggtctt atagaaaata atttccaaat atgttcttca gttagcggtt
     2221 tcaaaaaaat gaatgttaac gatgtttacg gtacgagctt tcacaataaa aataaagaat
     2281 cacgtatagt atcaattagc ggtacaagtt ttgtgccagg taaaacagca atagtgttcc
     2341 ttatgccaat agggaatgat atgacagctt ttagtattgt agagtcacga tatatctatg
     2401 atctaatgga tgttcttgat gatgagaatg atgattcatt ttctcttcgt tttactgaag
     2461 ggcctgcaat tattagcggt gtaaataata atgacaggtt atatatgtta aaaagagatt
     2521 ttaattctgc gatcagtcag gctcgtctta cagttactac gccgatgata tcgttatatc
     2581 cctatatcat tagcaatgtg ctttatattt tgccgttatc tattattctc tcttttattc
     2641 tctatttctt atggcagcac tggatgtcac ggaaaatgtc gctggcagaa gaaattaaaa
     2701 aaggaatgtc atccggagaa ttttctgtgc attatcaacc agtgtgcgac acgacaacca
     2761 aagcgtgtct gggggttgaa gcgttaatgc gctggcaacg cgaagatggt aaaaatatat
     2821 ctccggtagt ttttatccgc gctgcagaag aggaaaattt aataataccg ttaactaaac
     2881 atctctttga actgattatt caggatgtac aaagctggaa agtgaaaaag ccgtttcacc
     2941 ttgggataaa tattgccgcg gaacatcttg cacatcctga ctttgttggt gatgttttac
     3001 atattaaaaa tgcaatatct gataaattta atattgtcct tgagattacc gaacgcaatc
     3061 tggttgaaga cactgatctt gcattacaga aaataaatga attaaggatc catggctgtg
     3121 aatttgcagt cgatgatttt ggcacgggat attgttccct tggccttctg caaaagttgt
     3181 ctgttgatta tcttaaaata gataaaagct ttatagacac acttactaca gcggaagacg
     3241 aaacgccagt tttagatatt attattaagc tgagtaatcg gctcaatttg ataacgattg
     3301 cagaaggtgt cagtactcct caccagcaga atatcttatt cataataacg tgacattagt
     3361 tcagggatac ttatatgaaa aaccaatgaa agcgacagag ttttatcagt ggtatgtaaa
     3421 cagataaata attaaaaata ttggaatggt tttgtattgt cttaaaatat aaacaaagca
     3481 atcagaatgt tattaactga tgacaaacgc caagatcaac aatgctgtta gtgagtaata
     3541 catatgaatc gtggtgtcct gcctgtagag aatatgtagt tataatcatt ttgacgcgta
     3601 acataataat taattgaagt gtctgctttt atgaagaaat cagaagtgat attttcaagg
     3661 atttcatcgc tgatgttatc atctttttgt attttttgga ttttaactgt ggtgagataa
     3721 acggtatcac ggaatttctt gtatgtaaaa ctcacatatc ggcggatgaa gcccattttt
     3781 tttccatcta ccgtgtatgc gcccgtgaga tacatgtttc ctgtgttatt ttcagaaatc
     3841 atatttaacg aaaaatattg atccattttt gtatgatcgg tgaaattaat aacatgggca
     3901 ttacatgata tgttttgctc tgctggttca ttggaatgat gaaacgcaat atggctcaga
     3961 attgctgcaa taaaagcaca taaaattatt cccgaaaaca gaaaatatct ttgagtcata
     4021 tgattactcc gctttataat agagagagtt gcaattattt ggtgattcag agtttaacat
     4081 ttgactacag gtgaaagcat aaaacccgct catgctatca tttgttgcaa tgtaaatgta
     4141 aggatgtatt ttgcagttga cttccatttt ctgcaatttt tcaataattg gtgatagcat
     4201 gtctacggaa ttaaagtaat tattatcata gaaataactg catccttcct gagtagggtg
     4261 ttgagcataa tttgagaaag catctatttt ttttatagtg tgagatttgc tttgaaaagc
     4321 catatcgaaa aaataagaag acatataaac tatcccccat gccgcaaaaa aaaactataa
     4381 gtaatgtatt ctttgtggta aatatacctt ttgcaacgta atttttattt atattttcag
     4441 tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta ttgtcgagaa
     4501 cagttatgtt gatttcttca ttgattttga agcctttccg tggcaccgtt ctgata
//
LOCUS       DBKELA010000196         4550 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.200, whole genome shotgun sequence.
ACCESSION   DBKELA010000196 DBKELA010000000
VERSION     DBKELA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.200"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            13..1224
                     /locus_tag="ACUQR0_003996"
     CDS             13..1224
                     /locus_tag="ACUQR0_003996"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HHV9110815.1"
                     /translation="MFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYPLST
                     ALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISNYAM
                     PGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVTNIE
                     SDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKWFIA
                     LMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPVIVL
                     IMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLLIAN
                     KPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAARVF
                     VNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="ACUQR0_003997"
     CDS             1224..1898
                     /locus_tag="ACUQR0_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9110816.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="ACUQR0_003998"
     CDS             2426..3187
                     /locus_tag="ACUQR0_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HHV9110817.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3261..4550
                     /locus_tag="ACUQR0_003999"
                     /pseudo
     CDS             3261..4550
                     /locus_tag="ACUQR0_003999"
                     /inference="COORDINATES: protein motif:HMM:NF012786.6"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1249 a          864 c         1119 g         1318 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DBKELA010000197         4452 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.201, whole genome shotgun sequence.
ACCESSION   DBKELA010000197 DBKELA010000000
VERSION     DBKELA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.201"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..395
                     /locus_tag="ACUQR0_004000"
     CDS             <1..395
                     /locus_tag="ACUQR0_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HHV9110818.1"
                     /translation="LEYATTRKKSEVVYSGVSVTIPTAPTNLVSLLKTLTPSSGSLAP
                     FFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQLTFSGSVPDTLVSSRNAATT
                     TDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            395..850
                     /locus_tag="ACUQR0_004001"
     CDS             395..850
                     /locus_tag="ACUQR0_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HHV9110819.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            853..1461
                     /locus_tag="ACUQR0_004002"
     CDS             853..1461
                     /locus_tag="ACUQR0_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HHV9110820.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            1471..2802
                     /locus_tag="ACUQR0_004003"
     CDS             1471..2802
                     /locus_tag="ACUQR0_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HHV9110821.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            2803..>4452
                     /locus_tag="ACUQR0_004004"
     CDS             2803..>4452
                     /locus_tag="ACUQR0_004004"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HHV9110822.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTR"
BASE COUNT         1207 a         1107 c         1167 g          971 t
ORIGIN      
        1 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
       61 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
      121 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
      181 aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc cagcggaacc gccaacaggt
      241 caatgcagct aaccttttcc ggctctgttc ctgacacact ggtcagcagt cgtaatgcgg
      301 cgacaacaac cgacaacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
      361 ttgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
      421 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
      481 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
      541 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
      601 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
      661 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
      721 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
      781 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
      841 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgtgcgg cgaggaacct
      901 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
      961 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     1021 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     1081 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     1141 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     1201 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     1261 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct gcaaggtcag
     1321 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     1381 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gtattggtgc tggtatcgga
     1441 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
     1501 ttgttggctg gtatcggcgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
     1561 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
     1621 ctgactgctt tgcaaggtct ttcaggtatt gcgggtgttt ttcagcagga aaagcaggct
     1681 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
     1741 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
     1801 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
     1861 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
     1921 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc aacagaaccc gcggcagttc
     1981 ggcgaatttg tcgatcacct ggggatgaac agtctcgggc ccgaaaaata ctttgaccta
     2041 caggataaaa tgcagggtcg ccaggttacc atgcgcggtc aggatctgga ttcgcaaacc
     2101 gccgctcgga atcaggcaat cacaatgcgc ggacaagata tccaggcgaa tttaggtcag
     2161 cagcgcatta atctggacgc agaaacaaac cgcattaaca acgaaaataa gcgccttgac
     2221 cggatgctat cagcagaaac taacgacctg aagcgccagg aaatacagag ccgcatagca
     2281 gccaacaacc agcagttgca gcagaagcag caagcgctaa atgatggcta caaagacggc
     2341 atcaacaccc tcacaaccag catgttcact ctgaacgata tcgttagttc tccttcactt
     2401 aagagcatta caggcttacg tggagtaatc cccaacgttc caggctcaca ggctgcagac
     2461 actcaggcac gacttgatac ctttaaatca caagcatacc tgacagcggt tcaggccatg
     2521 cgaggcatgg gcgcactttc tgatgccgag ggcaaaaagc tcgaccaggc tgttggttcg
     2581 ctgcagaact cgcagagcga ggagtccttt cgtcgcaacg ctggcgtcat cctgaacacg
     2641 ctcaaccaga agcgtaatga ggcggttggt aagtacgttc agcaaaacgg tatcaagcga
     2701 gtggaagcgc ctcaggcttc tatagattac ctgaagcagc accccgagct gtcaatcgac
     2761 ttcattaatc gctacggata tcttccatct ttggggcagt aaatggctaa ttaccgtgat
     2821 ttgttagagc aggctggcgc acgttacggt gtgccagaag ggttgatgac tgcactgggt
     2881 gccaaggagt cttcttacaa ccctgccgca gtaagctccg ccggggctgt aggattgact
     2941 caggtcatgc ctgggacatg gcgtgatatg ggttataccg atgagcaaat gcaaaacccc
     3001 gaatatcagg ctgacgctgg cgcgcgctat ctggcaaaga tgtaccagca gtttggtaac
     3061 tggcgtgacg ctcttcaggc ttatcacgac ggtcccggca acgttatgaa ggcaaagcgt
     3121 ggtgaatata cgccaggacc tgaaggccgc ggttacgttg atgatcgctt tgctcaatgg
     3181 gctggtgacc cggtgacaga ctcaacagtc gaacagcgcg ccacctctgc aaaggtacat
     3241 cctcagcaag accctaacaa cccgtttgca caactggaag tacagtcatc cgaacaagta
     3301 tcggcatcag gtgtgcagtc agacccaaat aatccatttg ctcagattga gcagcaggca
     3361 gccagtcagc agccacctca acccgtaagt tctgtcgcac cgaaacctgt tcagcaacaa
     3421 acagttaatc aggccaataa tgcaccaaca cgggaagaac catcattgat gcaacaagct
     3481 ggcgattggc tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt
     3541 ctggtaaaca taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt
     3601 caggggcttg gggggccaaa agtattggat gatgtttatc gtccagtaga cagaccgaca
     3661 gacccctacg cgcaagctgg agagtcaata ggcggttatc ttgttcctgg agcaggtgta
     3721 gctggaaaca tggtcattgg ttctctcgct gacgcggcga atcaacgggg tgattttgcc
     3781 gaaaatgccg ctattaatgc cggacttagc attgctacgc atggcctgat aaatggcgtt
     3841 acccgtggtg ttcgtggtgc atcaaatata attagtggca ataaaacatc tgcacagaga
     3901 gcgaccactg cgccaacaga aacatcacca ttctccggtg atgccgctgc agcaacaaat
     3961 cctgcggttc atgccgcaga ggcaagagta gcacaaggtg taccaatgac gcctgcgacg
     4021 aagaacccag aggaagtcgt tcgcacagta gcagcacaaa aaaggccaaa tctcgcttca
     4081 tcgcttgatg aactagatat caatcctcag gctgaagttc tggagtctgc tgaaaggctt
     4141 aatgttgatt cattactccc ttcacacttt tccgggaacg agcaatacaa ggcagttgag
     4201 caagcaatca agtcccgtgc gggttctgct ctacaggtgc aggaaaatga agcaatcagg
     4261 cagctagcac agggcgcggg tgagataatt gatcgcgttt ccggtgcaaa agatgctctt
     4321 ggtatgagcg acaagtttat tgatacggtc aatggaagaa tgtctgcgct gatgaaacga
     4381 agcgaccagc tttatcgcaa tgttgaaaag gcgatgcctg caggtgcaaa aattgatgcg
     4441 ccatcaacaa gg
//
LOCUS       DBKELA010000198         4328 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.202, whole genome shotgun sequence.
ACCESSION   DBKELA010000198 DBKELA010000000
VERSION     DBKELA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.202"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..856)
                     /locus_tag="ACUQR0_004005"
     CDS             complement(<1..856)
                     /locus_tag="ACUQR0_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HHV9110823.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACUQR0_004006"
     CDS             964..1242
                     /locus_tag="ACUQR0_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110824.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACUQR0_004007"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACUQR0_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HHV9110825.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACUQR0_004008"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACUQR0_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HHV9110826.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACUQR0_004009"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACUQR0_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HHV9110827.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACUQR0_004010"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACUQR0_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HHV9110828.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..>4328
                     /locus_tag="ACUQR0_004011"
     CDS             4234..>4328
                     /locus_tag="ACUQR0_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HHV9110829.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1274 a          924 c          926 g         1204 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgctggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggtt
//
LOCUS       DBKELA010000199         4246 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.203, whole genome shotgun sequence.
ACCESSION   DBKELA010000199 DBKELA010000000
VERSION     DBKELA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.203"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            33..914
                     /gene="yddG"
                     /locus_tag="ACUQR0_004012"
     CDS             33..914
                     /gene="yddG"
                     /locus_tag="ACUQR0_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HHV9110830.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1169..2269
                     /gene="ompC"
                     /locus_tag="ACUQR0_004013"
     CDS             1169..2269
                     /gene="ompC"
                     /locus_tag="ACUQR0_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HHV9110831.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2433..2918
                     /locus_tag="ACUQR0_004014"
     CDS             2433..2918
                     /locus_tag="ACUQR0_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9110832.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3028..4246
                     /locus_tag="ACUQR0_004015"
                     /pseudo
     CDS             3028..4246
                     /locus_tag="ACUQR0_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT         1317 a          875 c          804 g         1250 t
ORIGIN      
        1 tttttaacgg caagagagac aaaacagcga gcatgacacg acaaaaagca acgctcatag
       61 ggctaatagc gatcgtcctg tggagcacga tggtaggatt gattcgcggt gtcagcgagg
      121 ggctcggccc ggtcggcggc gcagctgcta tctattcatt aagcgggctg ctgttaatct
      181 tcacggttgg atttccgcgt attcggcaga ttccgaaagg ctatttactc gccgggagtc
      241 tgttattcgt cagctatgaa atctgtctgg cgctttcctt agggtatgcg gcgacccgtc
      301 atcaggcgat tgaagtgggt atggtgaact atctgtggcc cagcctgaca attctctttg
      361 ccattctgtt taatggtcag aaaaccaact ggctgattgt accaggatta ttattagccc
      421 tggtcggcgt ctgctgggtg ttaggcggtg agaatgggtt acattatgat gaaatcatca
      481 ataatatcac caccagccca ttgagttatt tcctggcgtt tattggtgcg tttatctggg
      541 cagcctattg cacagtaacg aataaatacg cacgcggatt taacggaatt accgtttttg
      601 tcctgctaac gggagcatgt ctgtggattt actattttct cacgccacaa ccagaaatgg
      661 tatttagcac gcccgtcatg attaaactca tctctgcggc atttacctta ggatttgctt
      721 atactgcatg gaatgtcggt atattgcatg gcaatgtcac cataatggcg gtaggctcgt
      781 attttacgcc tgtactttcc tcagcgcttg cagccgtgct gctcagcgcc ccgctgtcgt
      841 tctcgttctg gcaaggcgca ctaatggtct gcggcggttc cctgctctgc tggctggcga
      901 cacgtcgtgg ttaaaataat tgccgggtct acggcccggc agttaatcag ataaattccg
      961 acaatattaa aaaataaatt tcaaatacca gtaattcacc ttatatctca tttcgacttc
     1021 gatcacatat cgataaataa tattattcta tttttctcat tgtttttaat caccattcta
     1081 ttttaaaaac agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt
     1141 ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac
     1201 tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa
     1261 actcgacctt tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga
     1321 tggtgataaa acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat
     1381 gattggtttt ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc
     1441 gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa attagtgaat ttggctctct
     1501 ggactatggt cgtaactacg gtgtcggcta cgacattggt tcatggaccg atatgttgcc
     1561 agaatttggt ggcgatacct ggagtcagaa agatgtcttc atgacatacc gtaccaccgg
     1621 tctagcaacc tatcgcaact acgatttctt tggcttaatt gaagggctga actttgccgc
     1681 gcaatatcaa ggcaaaaatg aacgtactga caacagtcat ctttatggtg ctgactacac
     1741 gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat gtttatgatg gctttggtat
     1801 cggtgcggta tataccaaat ccgatcggac aaatgcgcag gaaagagccg cagctaatcc
     1861 tctcaatgcc tccggtaaga atgcagaact atgggccaca ggtataaagt atgatgccaa
     1921 caacatctac tttgcagcta attacgatga aacattaaac atgaccacct atggggatgg
     1981 ttatatctct aacaaagcac aaagttttga agtggtggcg caatatcaat tcgacttcgg
     2041 cttgcgccca tcactcgctt acctgaaatc gaaaggcaga gatctgggcc gctacgccga
     2101 ccaggacatg attgagtata tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc
     2161 gacctatgtt gattataaaa tcaacctgat tgatgaaagc gactttaccc gtgccgtaga
     2221 tattcgcacc gataacatcg tcgctacggg cattacctat cagttctaac gtcctctcct
     2281 gaccagccct tctctgggct ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa
     2341 aaatgagcaa ttcttgatat ttctggtcat tcacctcaat taatattatt ccatatcctc
     2401 cggggacatc ttactataat atttattgat gagtgattat gaccaatatt aatacagctt
     2461 gtgtaaaaaa taatgccagt tatcaactta ataacgcatt acctaacaaa gaaaccatct
     2521 ccagcaattt ttgtgaacga ctagaacaat ggggtaataa atcggttaat aatggtgaag
     2581 aaagagcaat tgccgtggag cgaattaaag aagcttacaa ttcgaacatg gcatctcttg
     2641 atttgtcata tcttgatttg agtgaattac cgcctatccc ctcgacagtt aatacgttaa
     2701 atctggaaaa caattgtcta acttgtcttg acttagctga caatgccagt ctcgttaata
     2761 tcaacctcag cgttaataaa attaacacga taacctttcc aaaagaacca aaactggaaa
     2821 acatttatat tgatcataac aatctggaaa gtttagattt aaaaaatcag cattcattgg
     2881 ttaatctgga agcgcaaaat aatagcctaa aaaaatgata tttctgatag ttataaactg
     2941 aaatttctta atcttgatta taacaagcta gcatcgctgg atgtctcccg gcaagaatct
     3001 ctgattgagt taagtgccca tcacaatatg atcactgacc ttatattaca caatcatccc
     3061 aggatgaaaa caatcacttt aaacgacaac catattgcac atttaaacgc caaaaacact
     3121 acaaaactgg aatatttaaa cttaagcaat aacaatttac tgccaaccaa tgacattgat
     3181 caactaatat catcaaaaca tctttggcat gtattagtta acggcatcaa caatgatcca
     3241 cttgcacaaa tgcagtactg gactgcagta agaaatataa ttgatgacac taatgaagtg
     3301 accattgatt tatcaggact taatttaacc actcaaccac cagggctgca aaacttcacc
     3361 tctatcaatc ttgataataa ccaactcaca cattttgatg caaccaacta cgatagactc
     3421 gtaaagctaa gtctgaatag taatgctctt gagtcaataa attttcctca aggcagaaat
     3481 gtaagtatta cacatatatc tatgaataat aatgctctca gaaatattga tatagatagg
     3541 ctttcatcag ttacttattt tagtgcggca cataatcaac tagagtttgt gcaattagaa
     3601 tcttgcgaat ggctgcaata cctgaatctc agccataatc aattaactga tattgttgca
     3661 ggtaataaaa atgaactctt actgctagat ctatcccata ataaactaac aagtttacac
     3721 aatgtcttat ttcccaactt gaatacgtta cttattaaca acaatttgct ttctgaaatt
     3781 aaaatattct atagcaactt ccgcaatgtt cagacattaa acgctgctaa caaccagttg
     3841 aaatatataa atcttgattt cctgacttat cttccatcta tcaaaagttt aagactggac
     3901 aataataaaa taacccacat tgatactaat aatacatccg atattggaac tttattcccc
     3961 ataataaaac agagcaaaaa cttaaatttt ttaaatgttt ctgggaagaa caattgacct
     4021 actatgcagc tcatgttatt taatttgttt tccccagcac ttaagcttaa tactggcctg
     4081 gcaattcttt cgcctggtgc atttgaagtt cactctgacg gaatagatgc ggataacgaa
     4141 ttgtttcact atactattaa aaaagcatat accccatata atatacacac ttacaagaca
     4201 gaggaagttg taaaccagag gaatataaaa gttaaaaaca tgacct
//
LOCUS       DBKELA010000200         4198 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.204, whole genome shotgun sequence.
ACCESSION   DBKELA010000200 DBKELA010000000
VERSION     DBKELA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.204"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..158)
                     /locus_tag="ACUQR0_004016"
     CDS             complement(<1..158)
                     /locus_tag="ACUQR0_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HHV9110833.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACUQR0_004017"
     CDS             complement(308..469)
                     /locus_tag="ACUQR0_004017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110834.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACUQR0_004018"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACUQR0_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACUQR0_004019"
     CDS             914..1249
                     /locus_tag="ACUQR0_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HHV9110835.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1616)
                     /locus_tag="ACUQR0_004020"
     CDS             complement(1530..1616)
                     /locus_tag="ACUQR0_004020"
                     /inference="COORDINATES: protein motif:HMM:NF024464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HHV9110836.1"
                     /translation="MSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2161..2237)
                     /locus_tag="ACUQR0_004021"
     tRNA            complement(2161..2237)
                     /locus_tag="ACUQR0_004021"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACUQR0_004022"
     tRNA            complement(2339..2414)
                     /locus_tag="ACUQR0_004022"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACUQR0_004023"
     CDS             complement(2559..3380)
                     /locus_tag="ACUQR0_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HHV9110837.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACUQR0_004024"
     CDS             complement(3395..3751)
                     /locus_tag="ACUQR0_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HHV9110838.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4198)
                     /locus_tag="ACUQR0_004025"
     CDS             complement(3764..>4198)
                     /locus_tag="ACUQR0_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HHV9110839.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1161 a         1136 c          826 g         1075 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcggcgcaag attttttgtg cgctgtttca gtatgctggt ggcggtctgc tctcccgg
//
LOCUS       DBKELA010000201         4183 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.205, whole genome shotgun sequence.
ACCESSION   DBKELA010000201 DBKELA010000000
VERSION     DBKELA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.205"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..209)
                     /locus_tag="ACUQR0_004026"
     CDS             complement(<1..209)
                     /locus_tag="ACUQR0_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HHV9110840.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACUQR0_004027"
     tRNA            complement(324..399)
                     /locus_tag="ACUQR0_004027"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACUQR0_004028"
     tRNA            complement(406..480)
                     /locus_tag="ACUQR0_004028"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACUQR0_004029"
     tRNA            complement(597..681)
                     /locus_tag="ACUQR0_004029"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACUQR0_004030"
     tRNA            complement(690..765)
                     /locus_tag="ACUQR0_004030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACUQR0_004031"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACUQR0_004031"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HHV9110841.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACUQR0_004032"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACUQR0_004032"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HHV9110842.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACUQR0_004033"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACUQR0_004033"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HHV9110843.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1039 c          928 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtacgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DBKELA010000202         4125 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.206, whole genome shotgun sequence.
ACCESSION   DBKELA010000202 DBKELA010000000
VERSION     DBKELA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.206"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..413
                     /locus_tag="ACUQR0_004034"
     CDS             <1..413
                     /locus_tag="ACUQR0_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110844.1"
                     /translation="KIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYK
                     AIDQVRAEGQQLVNQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTE
                     WKKQEKDFQQFGKDVCSRVVTLEDSRKALVGNLK"
     gene            589..1635
                     /gene="ansB"
                     /locus_tag="ACUQR0_004035"
     CDS             589..1635
                     /gene="ansB"
                     /locus_tag="ACUQR0_004035"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HHV9110845.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            1752..2713
                     /locus_tag="ACUQR0_004036"
                     /pseudo
     CDS             1752..2713
                     /locus_tag="ACUQR0_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(2814..>4125)
                     /locus_tag="ACUQR0_004037"
     CDS             complement(2814..>4125)
                     /locus_tag="ACUQR0_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HHV9110846.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFARTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKL
                     ILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLI
                     VGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCS
                     ISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         1269 a          951 c          993 g          912 t
ORIGIN      
        1 ataaaattat cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac
       61 ttgatgcgca gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga
      121 cgttccacta taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg
      181 caatgggcgg aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa
      241 gcggcggtaa cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc
      301 aaaccgagtg gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc
      361 gcgttgtgac tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta
      421 ttttaagacg gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct
      481 aactttgtag atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat
      541 gcaaagctaa gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc
      601 aaaaagacgg cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc
      661 aatatcacca ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa
      721 tctaactaca cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta
      781 aaagacattg cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac
      841 gataatgtct ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc
      901 ttcgtcatta cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg
      961 gtgaaatgcg acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc
     1021 gcagacggtc cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct
     1081 aatcgtggcg tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa
     1141 accaacacca ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt
     1201 cacaacggta agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca
     1261 ttcgatgtct ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac
     1321 gcatccgatc ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct
     1381 ggtgtgggta atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa
     1441 aacggcactg cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct
     1501 gaagtggatg atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg
     1561 cgcgtcctgc tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt
     1621 ttcaatcagt actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt
     1681 acgtccattg ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg
     1741 agggttttcg ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg
     1801 gtaatgcctg ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa
     1861 agcagtatca cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg
     1921 gtaatcaggc cacttggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct
     1981 gggtcggtca gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg
     2041 taaaattctc ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact
     2101 tttactcttt tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga
     2161 ataataaata tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat
     2221 ctgcgttgaa tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg
     2281 ataagcaaat ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg
     2341 gtaagcaaat gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta
     2401 ataaacagaa cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg
     2461 cgattgcggc atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac
     2521 gagatttcga tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc
     2581 gtaaattgca ggatgactgg gtaacgctcg aaaagggcaa tatccgttaa cgatgaaagt
     2641 ttcagaaata aacagtaaaa aagtcgcgat tttgatgaaa attgacgata tcaaccaagc
     2701 taatgaacgc tagaaaaaag ataccgaaca actgcggcgc aatggggtga ttaagtaact
     2761 catgttaagt gcctttgccg gataatataa aaagttatcc ggcaaattat tttttacatt
     2821 agccctagta gctgtggaat gaacaaggat atttgcggaa tgtaagtgat catcatcaac
     2881 gctactagca atgcagcata aaatagcagc aatggtttga ttaaatgttg aatcttcaca
     2941 cccgagatag aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata
     3001 caaaggttgg ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca
     3061 atcggcaaga agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg
     3121 acaattaata agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc
     3181 atcaatgcgt cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc
     3241 acggaaaaac cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt
     3301 ttgggtaaat ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag
     3361 acaacggcaa ttgctgacgc ttcagttgcg gtaaaaatgc cgcccagaat gccgcccaga
     3421 atgccgccca tcacaatgaa caccagaagc aaacttggta aggcatccag aaacgcttta
     3481 caggcctgag caaaggttgc tctttctgaa agcgggtagc cacggcgttt agcaataatg
     3541 ccgcagacta tcattaccgc aagacccatt aagatccctg gcaaatatcc cgccataaac
     3601 agcgatgcta cagaaacgcc gccagctgtc agggaaaaca caattaacac gtttgagggc
     3661 ggtatcagta atccagtaat acaagatgag acgtttacag cggtagaaaa tgccggatca
     3721 taaccttctt tggtctgaat gggattcagt gtgccaccaa cggcagcagc tgccgcaacg
     3781 gctgaaccag aaatggaacc aaacatcata ttcgccagga cgttcacatg accaagcgaa
     3841 ccaggaacgc ggccaaccat tacctgggcg aggttaatca aacgtatcgc aataccgccg
     3901 ctgttcatca gtgttctggc aaagatgaaa aaggggattg ccaacagggc aaagttatcc
     3961 agtccattag ccagtcgttg cgaaacagtg attgccgcga tatcccaggg aaacatcaaa
     4021 agcattgaag ctacagtggc aataccgatt gagaacgaaa tgggcacacc aataaagacc
     4081 agcacaaaaa aacagccaaa cagtgtaatc gcgatataac tatcc
//
LOCUS       DBKELA010000203         4045 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.207, whole genome shotgun sequence.
ACCESSION   DBKELA010000203 DBKELA010000000
VERSION     DBKELA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.207"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..358
                     /locus_tag="ACUQR0_004038"
     CDS             <1..358
                     /locus_tag="ACUQR0_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HHV9110847.1"
                     /translation="VFVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            620..982
                     /locus_tag="ACUQR0_004039"
     CDS             620..982
                     /locus_tag="ACUQR0_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HHV9110848.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            979..1899
                     /locus_tag="ACUQR0_004040"
     CDS             979..1899
                     /locus_tag="ACUQR0_004040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHV9110849.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1902..3422
                     /locus_tag="ACUQR0_004041"
     CDS             1902..3422
                     /locus_tag="ACUQR0_004041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HHV9110850.1"
                     /translation="MSICIKQSALKILLALSALLITWLTTRYFPVEPDVANSPIVWRH
                     ILENGISSIHDWKPTVDNWYFTVYPINFLFYMLLGDDGLVALRLSTAVFSIAIVIAAM
                     LTLRKAFGFTPALFSIILLSLIPYFSYTYGFVSHPFSHNSTNAFGFLCLLISVFNIQY
                     KNIFITLLLSLTALFSSVSDPWFTAAFFIPLLISYFLFSVWDKKLFKHTALILFACLI
                     SLSNVLQNLLNIPPHQFEIVSLNDMILNAKWCILLIGKSLNLLVVDNNATSYASFVIW
                     FIAIITSAWFVLSDNKKNTYRIYIVLFSLLSIAGIVSSFILSYKSPDYISMRFFMNVT
                     CFALILCCIGTSTKAKILFYLIAFLFSISSIKSYTNNASPLHDQEKIVKSYIDFLKKN
                     NLHYGYGSFWDLSMTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAFN
                     SAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3656..>4045)
                     /locus_tag="ACUQR0_004042"
     CDS             complement(3656..>4045)
                     /locus_tag="ACUQR0_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9110851.1"
                     /translation="ASTNGFYSTEFHGTNIPDDAVEISESEWKTLINSQSVTKMITCG
                     ENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQDAVDLDMATDDETKLLL
                     AWKKYRALLMRVDIKNTEWPKKPESNL"
BASE COUNT         1169 a          793 c          785 g         1298 t
ORIGIN      
        1 ggtgtttgtc cacaccaaac ctgcctaccg aagcgacgga ataaaaacag cagcggtaag
       61 gaagatgaga accgacagca acaaggcatg gaagggagcg ttaaagcggg caggcattag
      121 caacttccgc ttccatgacc tgaggcatac ctgggcaagc tggctggttc agtccggtgt
      181 ctctcttctt gcgcttaaag agatgggagg atgggaaact ctcgaaatgg ttcaaagata
      241 cgcccacctt tcagccgggc atctcaccga gcacgcgagc aaaatcgatg cgattataag
      301 tcgcaatggc acaaatacgg cacaagagga gaacgtagtt tacttaaatg cgaggtaact
      361 tattgattta aatggtgccg ataataggag tcgaacctac gaccttcgca ttacgaatta
      421 taagaatccg cttgtaattc aaaggattat cccatcaaca ctgcgctcac acgtcccacc
      481 acatcaaaac atgtaaagcc ttgcaagcca ttgtgaggcc ttatgtgtct cagttttgtc
      541 tcatcagaca tagcaagtat cgatcaattg agacttggat gatagacttc atgcctttca
      601 gagctcattg attaaataaa tgttaaagtt atttgtaaag tacacatcta taggtgtgct
      661 gaacacactt atacactggg tggtttttgg tgtatgtatc tataccgcac ataccagtca
      721 ggctctggca aacttcacag gtttcgtagt ggctgtgagc tttagcttct tcgcgaatgc
      781 aagattcaca ttcaaagcat cgactacagc gatgcgctac atgttatacg tgggatttat
      841 gggaatattg agtgtgattg ttggttgggc agctgataaa tgctcacttc ctccaatagt
      901 cactcttatc accttctccg ccatcagtct ggtgtgcggt ttcgtctatt caaagttcat
      961 tgtctttagg gatgcgaaat gaaaatatct cttgtcgttc ctgtcttcaa tgaagaagaa
     1021 gcgatacctg ttttctataa aacggtacgt gaattccaag agttgaagcc atatgaagta
     1081 gaaattgtat tcataaatga cggaagtaaa gatgccacag agtcaattat taacgcgctg
     1141 gctgtttcag acccgctagt tgttccgctg tcatttacac gcaactttgg taaagaacca
     1201 gccttatttg cagggttaga ccatgcaagc ggcgatgctg taattcctat tgatgtcgac
     1261 ctgcaagacc caattgaggt tatccctcat cttattgaaa agtggcaggc aggtgctgag
     1321 atggtgcttg ctaaacgttc agaccgctca actgatggac gactgaaacg taagacagct
     1381 gagtggttct ataaattaca caacaaaata agcaccccaa agatcgagga aaatgtcgga
     1441 gattttcgac tcatgtctcg tgaggttgtg gagaacatta aactgttgcc tgagcgcaat
     1501 cttttcatga aaggcatact gagctgggtg ggtggtcaga cggatgtcgt tgaatatgta
     1561 cgcgcagagc gtgttgctgg catctcaaaa tttaatggct ggaaattatg gaatctggca
     1621 ctggaaggta tcacaagttt ttcaaccttt cctcttcgcg tatggactta tataggcttg
     1681 tttgttgcaa gcatttcatt tttatatggt gcatggatga ttatagacac ccttgtcttt
     1741 ggtaacccag tacgcgggta tccctccctg cttgtatcaa tacttttctt gggtggagtg
     1801 caactgatcg ggattggtgt tctcggtgaa tatataggta gaatttactt agaaacaaaa
     1861 tcaagaccta agtatatttt gaagaaaggt agaaaataac catgagcata tgtattaaac
     1921 aatcagcatt aaaaatatta ttggctttat cagcactcct aataacttgg ttgactacca
     1981 gatactttcc agttgaaccg gatgttgcta acagcccaat tgtatggagg catattttag
     2041 agaatggtat atcatcaatc cacgactgga aaccaaccgt ggataattgg tatttcaccg
     2101 tttacccaat taacttttta ttttatatgc tgttaggtga tgatggctta gtagcattaa
     2161 gattatcaac tgcagttttc tctattgcta tagtaatagc agcgatgtta acgctacgta
     2221 aggcattcgg gtttacccct gctttatttt ctattatact tctatctcta ataccatatt
     2281 tttcatacac ttatggtttt gtatcacatc ctttttctca taattcaaca aacgctttcg
     2341 gctttttatg cttgcttata tctgttttca acatacagta taaaaatata tttatcactc
     2401 tcctcttaag tttgacagca ttattttcca gcgtatccga tccgtggttt acagctgcat
     2461 tttttattcc actattaata tcttactttt tattctctgt atgggataaa aaattattta
     2521 agcatactgc attaatacta tttgcatgtt taatctctct ttccaatgta ttgcaaaatc
     2581 tacttaacat acctccccac caatttgaaa ttgtatcact caatgatatg attttaaatg
     2641 caaaatggtg cattctgtta ataggaaaaa gtcttaattt attagttgtc gacaataacg
     2701 caacatctta cgcctcattt gtcatttggt tcattgccat tattacatct gcgtggtttg
     2761 ttttatcgga caataaaaaa aacacatacc gtatttatat tgtgttattt tcattattat
     2821 ctatcgcagg tattgtctca tcattcatac tgagctataa atcccctgat tatattagca
     2881 tgcgtttctt tatgaatgtt acctgtttcg cactgatatt gtgctgtatc ggtacatcaa
     2941 caaaagcaaa gatattattt tatctaatcg catttttatt ttcgatcagt tcaattaaat
     3001 cttacacaaa taatgcttca ccattacacg atcaggagaa gattgttaaa tcatacattg
     3061 attttttaaa aaaaaataat ctccattatg ggtacggctc tttttgggat ttgtcaatga
     3121 ctgtaaattg gttatctggt ggggacatac agataactcc tgttttcttt aatgctgatt
     3181 cggggaaaat taactttact ggcgtgagac aacagacact ggcttcttgg catagcaagg
     3241 aggcgttcaa ttctgctcct gagcgacagt ttatagcagt ttccatagct aacgaacctg
     3301 aacgatgtaa agaaatgaca tcctgccttg caggtattca agaacaatta ggtaagcctg
     3361 acgaagtttt gaactttgaa ggaagagtaa tattagtatt taacaaaaaa cttaacctat
     3421 agtaaattgc cccctaccat ataaaccaca ctaacgaatg aataacccgt ctttttcgac
     3481 gggttatatc cctatcagga ataaaaatgg agtggttgcc gtatacaaca aaccctcatt
     3541 tagagctata ttctagagta tatgagatac tataaagttg aaggagctat cattagaaaa
     3601 accatatgct gttataatta gatgtctcgc ttttcatcca ataacgaatt ttctcttata
     3661 gattgctctc tggttttttc ggccactctg tattttttat atcaacacgc atcaatagtg
     3721 cgcgatattt tttccatgcc agtaacagtt tcgtttcatc atctgttgcc atatctaaat
     3781 caaccgcatc ctgaagcggc gctattacat tcgttgcctc tgctatcagt gcagatttct
     3841 tttcattatt tattaaggct aattgttctg gtgttggaga aggacggtca acaatgacag
     3901 ggtgaccatt ctcaccacag gtaatcattt ttgttacact ctgcgaatta atcagtgttt
     3961 tccactctga ttccgagatt tccactgcat catcaggaat attggtgccg tgaaattcag
     4021 tcgaataaaa tccgtttgtc gatgc
//
LOCUS       DBKELA010000204         4026 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.208, whole genome shotgun sequence.
ACCESSION   DBKELA010000204 DBKELA010000000
VERSION     DBKELA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.208"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(6..197)
                     /locus_tag="ACUQR0_004043"
     CDS             complement(6..197)
                     /locus_tag="ACUQR0_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HHV9110852.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            261..584
                     /locus_tag="ACUQR0_004044"
     CDS             261..584
                     /locus_tag="ACUQR0_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HHV9110853.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            574..1418
                     /gene="mbeA"
                     /locus_tag="ACUQR0_004045"
                     /pseudo
     CDS             574..1418
                     /gene="mbeA"
                     /locus_tag="ACUQR0_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxase MbeA"
     gene            1305..1634
                     /locus_tag="ACUQR0_004046"
     CDS             1305..1634
                     /locus_tag="ACUQR0_004046"
                     /inference="COORDINATES: protein motif:HMM:NF016710.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HHV9110854.1"
                     /translation="MKERAESTEKMLNDEFRKLEESVNRELTSNESLIRNAINDHTSA
                     LKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTGSLLWYQGMKINA
                     NMNILRE"
     gene            1674..1793
                     /locus_tag="ACUQR0_004047"
     CDS             1674..1793
                     /locus_tag="ACUQR0_004047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mbkB"
                     /protein_id="HHV9110855.1"
                     /translation="MRYHEDSNGRFLVLPEGMKAETNWTMENGKQNAVRLVQE"
     gene            1800..2033
                     /locus_tag="ACUQR0_004048"
     CDS             1800..2033
                     /locus_tag="ACUQR0_004048"
                     /inference="COORDINATES: protein motif:HMM:NF016769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HHV9110856.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2579..2971)
                     /locus_tag="ACUQR0_004049"
     CDS             complement(2579..2971)
                     /locus_tag="ACUQR0_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110857.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNNTLWETAKQQLLQKSDGRYFAINSKHLSFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(3288..3446)
                     /locus_tag="ACUQR0_004050"
     CDS             complement(3288..3446)
                     /locus_tag="ACUQR0_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHV9110858.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
BASE COUNT          985 a          911 c         1168 g          962 t
ORIGIN      
        1 atatgtcaga ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca
       61 tcggcgtggt cgtgaaacga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
      121 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
      181 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgttcag gaggtaatga
      241 taccgatgaa acgcgagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
      301 tactggaacg ctgtgagggt aaacagctgg cggtatggat gcggcgggtc tgcctggggg
      361 agccggttgc ccgttccggg aaactgccga cactggcacc gccgttactg tgtcagctgg
      421 ctgccatcgg gaataacctg aatcagacag cgaggaaggt gaacagcggg cagtggtctt
      481 ccggtaaccg ggttcaggtg gtggccgcac tgatggccat cggggacgag ctgcgccggc
      541 tgcgtctggc cgtcagggaa caggggacgc gggatgatag ttaagtttca tgcccgggga
      601 aaaggtggtg gcagtggtcc ggttgattac ctgctgggaa gggagcgtaa ccgggaaggg
      661 gcaacggtgc ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc cacgccattt
      721 gcgaagaaat acacgtccgg tgttctgtcg ttcgcggaga aggagctgcc gccgggagga
      781 cgtgaaaaag tgatggcgag ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag
      841 tacagcatcc tgtgggtgga tgcaccagga caagggacgg cttgagctga attttgtcat
      901 tccgaacatg gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag
      961 gcctagaatt gatgcctggc agacgctggt taatcactat tacgggctgc atgacccgaa
     1021 cgccccggag aaccgcagga cgctgacact ccctgataat ctgccggaaa cgaaacaggc
     1081 gattgctgag ggcgttacac gcgggataga cgcgctttac catgccggag agataaaagg
     1141 ccgtcaggat gtgattcagg ctctcacaga ggcagggatt gacgtggtta gggtgacgcg
     1201 aagcagtatc agcattgcag acccgaacgg cgggaagaat atcagactga aaggagcgtt
     1261 ttatgagcaa tcttttacag acgggtgcgg agttcgagaa aaagctgaaa gagagagccg
     1321 aatctacaga gaaaatgctg aacgacgagt tcaggaagct cgaagaatct gtaaacaggg
     1381 aactgacctc aaacgaatct ttaatcagga acgctatcaa cgaccataca tcagccctga
     1441 aagaacttct ggagaggtat cagaaaacga ccgtcgatac gatggacgca cactggaaaa
     1501 ccgtactgaa aatgagcgtg aaacgctggc tgtggctgat tatcgtctca gtactgatgt
     1561 ttgccacgac cgggagcctg ctgtggtacc agggcatgaa gataaacgcg aacatgaata
     1621 ttctccgcga gtagaaagag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt
     1681 atcacgagga cagcaacgga cgttttctgg tgttgcccga ggggatgaaa gccgaaacga
     1741 actggacgat ggaaaacggg aaacagaatg ccgtaaggct ggtacaggag taacagagca
     1801 tgacggagct ggaaagacat ttgctgaacg cattagaaca actgcaacag gactatatgc
     1861 agcggctgaa cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc
     1921 aacgggacaa cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgcagcact
     1981 tgagcgagca gacagaacgc ttgagccagt tatacagcga gaacaggaga taagggacga
     2041 acggctgata caggaatgcg aacatgcgtt atccctggag cgtgagcacc agctggaaat
     2101 acaggaacgg acactggatg gcccttcgct gggatggtaa tcagagacag gctaaaatgt
     2161 tgtcagcgcg tttccggtgc attttttggg ataatggttg gttcgggggc accgggaacg
     2221 cgcttcagtc agaccttgct gaaaacatag gaacgcgtta aaacgcatac gggcgcgatt
     2281 ctggtgtgtc ttttgttgct gtgttttgcc tctattgcca gatcgcgctc attcccctga
     2341 acttcttttt tcccgctcag gtccccgtct tcttcgcttc cgaaagcatg aaaaaaatcc
     2401 gtttcagctg tctctggctg gcaattcctg cgtgattcac atggctgcat agctatgcat
     2461 gtggttaaaa tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt
     2521 acctgtctgc tgccgtgatc gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt
     2581 acgttaaatt gtgagggaat ggtccccata tactgacttt atcataggga tagggttggt
     2641 agtgggtcga ttgggtttcg gcattgtttc cgttaatgcc aattgcattt ttgtatttga
     2701 tgcaacagaa ggcgtgagat tctcctgggc ctggaaagga taagtgttta gaattgatgg
     2761 caaaatatct tccatccgat ttttgaagta gttgttgttt ggctgtctcc caaagggtgt
     2821 tgttacaaac ttcttgaatt tgtagttttt gcaggactct tattactgcg ttatcatgtt
     2881 ccaattcttt aggggagtgt attattctct cttttaataa gctgtttacc acagggttta
     2941 gttgttgtct aaagcacgca gataatgcca ttgctacaca attttgttcc cgaggaagca
     3001 tgtttggcgg ggtatgccgt gtttgatgtg atatgtctat tctcatagaa ggtctctggc
     3061 ttatagcatc aacaaatacg gctataaaac aaaattttca tagagtgatt caagatcttc
     3121 ttgagatcct tttttgcggc gcgtaatctg ctgcctgcaa acaaaaaaac caccgctacc
     3181 aacggtggtt tgtttgccgg aatctagagc taccaactct ttcttccgaa ggtaactggc
     3241 ttggtggagc acagatacca aatgctgtcc ttctagtgta gccgcagtta ggccaccact
     3301 tcaagaactc ttaatatctc aatccacctt gtccagttac caatggctgc tgccagtggc
     3361 gttaagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3481 ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcgg
     3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccatcgg cgcggccttc
     3721 ttcctgtccc tggtcttttg ctcacatgtt ctttccggcg ttatcccctg attctgtgga
     3781 taacagcatt tcgtttttga gtgagtagat accgctcgcc gcagtcgaac gaccgagcgt
     3841 agcgagtcag tgagcgagga agcggaaaag cgcctggacg tgcattttct ccttacgcat
     3901 ctgtgcggta tttcataccc ggtatggcgc acttttcata caatctgcac tgatgccgca
     3961 tggttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     4021 acccgc
//
LOCUS       DBKELA010000205         3590 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.209, whole genome shotgun sequence.
ACCESSION   DBKELA010000205 DBKELA010000000
VERSION     DBKELA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.209"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            32..493
                     /locus_tag="ACUQR0_004051"
     CDS             32..493
                     /locus_tag="ACUQR0_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HHV9110859.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="ACUQR0_004052"
     CDS             complement(534..1004)
                     /locus_tag="ACUQR0_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HHV9110860.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="btsR"
                     /locus_tag="ACUQR0_004053"
                     /gene_synonym="yehT"
     CDS             complement(1051..1770)
                     /gene="btsR"
                     /locus_tag="ACUQR0_004053"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HHV9110861.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKNVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="ACUQR0_004054"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="ACUQR0_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HHV9110862.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          925 c          817 g          895 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacattttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DBKELA010000206         3586 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.210, whole genome shotgun sequence.
ACCESSION   DBKELA010000206 DBKELA010000000
VERSION     DBKELA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.210"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1412
                     /locus_tag="ACUQR0_004055"
     CDS             <1..1412
                     /locus_tag="ACUQR0_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HHV9110863.1"
                     /translation="LPASRVYPGSSRQAYLANRALHELRKNRTLPVDEAWVDDFVREA
                     FGRMKKEYVCKDSEMATEGATDLWEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQ
                     NAENVSEYLSLRHQGLQQSIQSLQSHITSLLADIREIADCQEQVTADVRMAMEEIDAR
                     TRELLTGVCTSLEEELNDYFRSGKRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQH
                     DRMRDFDPDSPEIKFSDRREALELMTQIESTVTSLHREAEAQFCPELEKIVRGIETGF
                     RGTALYATENIAGRINARLEDEGFTVKISFPAVSQLQTRLAVKTNLSALMEERTETVT
                     RRCRQDGVWGTLCRWANTSDWGWKEYSVDVSRSVININKVREEVMSLTRAYFGELQAS
                     IEQDINQPVRQEIDAFFCTFREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNER
                     VPELITDSKALREELETML"
     gene            1409..2314
                     /locus_tag="ACUQR0_004056"
     CDS             1409..2314
                     /locus_tag="ACUQR0_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HHV9110864.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2311..3381
                     /locus_tag="ACUQR0_004057"
     CDS             2311..3381
                     /locus_tag="ACUQR0_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HHV9110865.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT          904 a          842 c         1036 g          804 t
ORIGIN      
        1 tgctgcccgc ctcccgcgtt tatccaggct catcacgtca ggcgtacctg gctaaccgtg
       61 cgcttcatga gttacggaaa aacagaaccc ttcctgttga tgaagcctgg gtcgatgatt
      121 ttgtcaggga agccttcggt cgcatgaaaa aagaatacgt ctgtaaagac agtgaaatgg
      181 caaccgaagg ggcgacagac ctgtgggaag gctcacttat cgatcaactg ataacggaag
      241 tcatacagag ctcccattcc agagcggcgg cactggcggt tgattctgcc gccgcaaaac
      301 tgatgcagaa tgcggaaaat gtcagcgaat atctgtcatt acgccatcag gggctacagc
      361 aaagcattca gtcactgcag tcgcatatca ccagcctgct tgcggatatc cgggaaatcg
      421 cggactgtca ggagcaggtg accgctgatg tcagaatggc catggaggag atcgatgcca
      481 gaacccggga attactgacg ggggtctgca cctcactgga agaggagctg aatgactatt
      541 tcagaagcgg taaacgcaaa gaacagcaaa tgctggagga agaaaattca gcgcagcccc
      601 gggagagaaa cgcattcgct ttctttcacg atattttcgg aaccggaaat cagcatgaca
      661 ggatgcggga ctttgatccg gacagccccg aaataaaatt cagtgatcgc agggaagccc
      721 ttgaactgat gacgcaaatc gaatcgaccg tgaccagcct gcaccgtgag gctgaagcac
      781 agttctgtcc tgaactggaa aaaatcgtca ggggtattga aacaggtttt cgtggtacgg
      841 ccctgtacgc cacagaaaat attgccggac gcatcaatgc ccgtctggag gatgaaggtt
      901 tcactgtaaa aatcagtttt ccggcagtca gccagttaca gacccggctc gcagtaaaaa
      961 caaatctgag cgcgcttatg gaggaaagaa cggagacagt aacccgtcgc tgtcgacagg
     1021 atggtgtctg gggtacgctt tgtcgatggg caaacaccag tgactggggc tggaaagagt
     1081 acagtgtgga tgtgagccgc agcgtgatca atatcaacaa ggtcagagag gaagtgatgt
     1141 cactgacccg ggcatatttc ggggagctgc aggcatccat tgagcaggat attaaccagc
     1201 ccgtccgcca ggaaatcgat gcctttttct gtacattcag ggagaaagtt gaacagctgc
     1261 gtaacacgct gattcagagc tctgaagatc ataaacgcga tcagcaggcg caggaacggc
     1321 tgaccgggcg tcttcaggcg ttaaatgaaa gggttcctga gctcattact gacagtaagg
     1381 cgttaaggga agaactggag acaatgctgt gacttcacca tttattcaac aaattgctga
     1441 taaccgggta tgtcaggtac tcacctgtct tcctgaaaaa tttgtggttg atttcgcaaa
     1501 cggtattgat gttgcacagg agcatatccg tacggccgga gagcgcacgt tcttccggcg
     1561 gttaaaagag gggctgagcg gcgaaggtac tgcacggcag aatgccatca atgcttcgct
     1621 tgcacagggc gttgaggcgt ccctgcgctg gctgacggag ctgacaacgt cacttgccac
     1681 cacaaactac gcgattaccc gggtaaacga cagggtcagt tcactggtca gtgatactgc
     1741 caggctggcg cattattccg cagatacgcg ggagcagtta ctcaccctgg ccgatcaggt
     1801 tcaccagaaa ctgaatcatc ttgaagaaaa actccaccgt gttgacctgg ttcagcgggc
     1861 gcagttacat cttgagcaga tattctcctg gtggagcgcc gggcgatatg cgtcattttc
     1921 ccctgccgga cgttgttatg tggcacttga agagcttcgc tggggcgcgt ttggtgatgt
     1981 gatacgtcag ggcgaaacag gccaggttaa ccagctactg gatattctca gacataaagc
     2041 attaacgcag atggcacagg agagtggcgg tagtgcaacc gtgcgtctga acactctgga
     2101 ctggcttggt ggtcagggcc gggagcaggc agataacgag tggcatgacg cgattaactg
     2161 gctgggggac tggtgcagtg aagagcagca tccggtgatc tggtcaacca cgcaggctgc
     2221 agaacactta ccggttcgca tgccccgtct ctgctctgca gaacgcctct ctgaaagtat
     2281 ggtcgatgaa atatttcaga agggggctgc atgagtatag aaataaaaac agggctggtg
     2341 ctgtccggcg gtggggcggt gggcgcttat caggcgggag tggttaaggc actggcagag
     2401 tgtggtacac agatcagcat ggtttcaggg gccagcattg gcgcattcaa tggtgccatt
     2461 atcgcggcct ctcccgatct gtcagaagct gccgtacgcc tggaggcgct ctgggatcat
     2521 cttgggaata atcaggtgct gtcggtaaac cgatcggttt acttttcatt gctgaaaaaa
     2581 ttgttccagg caatgaacct ctgccagatc cccggacgtg caggagcact gcttacgaca
     2641 cttcttcgcc atatatcgat aatcaacggc ttcgacaatc cgatgactca gccgttgttg
     2701 tcagatgggc ccctgacagc gttgatggat cattatcttg atacagatgc tctggcaggc
     2761 gggctaccgc tgtatgtgtc gctgtacccc acagaagggg gcatgcagga tattattgac
     2821 tgcattcgtg ctgaactggg ttccggaacc acgaaaaacg ccgtttttca gcatatccag
     2881 agtctgcccc gcggacagca gaaagaggct ctgcttgcgt cagccgcact gcccctgttg
     2941 ttccgtcccc gtgaggttca ggggacaatg ttcggtgatg gtggtatggg aggatggcga
     3001 aatatgcagg gaaatacccc tgtaacgcct ctggtcgatg ccggatgcaa tatggtgatt
     3061 gtgtcgcatc tgagtgacgg ttctttatgg gatcgccagg cttttccgga caccacaatc
     3121 cttgagatcc gtccccggaa aaggctgaaa tatgcaggtg atggtggcaa cagtggcggt
     3181 ctgctcagtt tcacgtcggc acataccgac gcctggcgtc agcagggcta cgaggacacg
     3241 atgctgacga tggagcatat ccggaaaccg ctggcagcac gtcaggcact gtcccggtca
     3301 gaaacggtgt tgcagaaaag tctggagata acggaagagg cagatttggc actgagaagc
     3361 gcgatggccc ggattaaata aagacgcccc aaagaaacgc caccgcctgt ggtggtgttt
     3421 ttcctgtctg gtgttgttga aaatattttc agatatatat tacctgggtg agatttaaga
     3481 ttaacgcatt tattaatgtc ataaggagag ttatcatgaa attcatcgta aaagcactta
     3541 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttga
//
LOCUS       DBKELA010000207         3570 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.211, whole genome shotgun sequence.
ACCESSION   DBKELA010000207 DBKELA010000000
VERSION     DBKELA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.211"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            156..434
                     /locus_tag="ACUQR0_004058"
     CDS             156..434
                     /locus_tag="ACUQR0_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110866.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            436..1494
                     /locus_tag="ACUQR0_004059"
     CDS             436..1494
                     /locus_tag="ACUQR0_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HHV9110867.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            1495..1860
                     /locus_tag="ACUQR0_004060"
     CDS             1495..1860
                     /locus_tag="ACUQR0_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HHV9110868.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            1857..2545
                     /locus_tag="ACUQR0_004061"
                     /pseudo
     CDS             1857..2545
                     /locus_tag="ACUQR0_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            2739..2814
                     /locus_tag="ACUQR0_004062"
     tRNA            2739..2814
                     /locus_tag="ACUQR0_004062"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2772..2774,aa:Ile,seq:cat)
     gene            2816..2892
                     /locus_tag="ACUQR0_004063"
     tRNA            2816..2892
                     /locus_tag="ACUQR0_004063"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2850..2852,aa:Lys,seq:ttt)
     gene            2895..2970
                     /locus_tag="ACUQR0_004064"
     tRNA            2895..2970
                     /locus_tag="ACUQR0_004064"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2928..2930,aa:Thr,seq:tgt)
     gene            2974..3048
                     /locus_tag="ACUQR0_004065"
     tRNA            2974..3048
                     /locus_tag="ACUQR0_004065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3007..3009,aa:Gly,seq:tcc)
     gene            3341..3556
                     /locus_tag="ACUQR0_004066"
     CDS             3341..3556
                     /locus_tag="ACUQR0_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HHV9110869.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          897 a          847 c          954 g          872 t
ORIGIN      
        1 gggagaaatc cctcgccacc gctgatgtgt caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg ggttttgttt tttctggtcg ttctggttta caatccatcc gtcagcctga
      121 acaactggca cctgctgcgc cagcagagaa aacagatggc gcacgatacc aaattttaca
      181 attcggataa ctctgccgcc cctgccagca ggcacgggcg gcgttctcat gcattcaaat
      241 ctgacgggta tcagcacgac ccctgcaccg aagaacaggc tgaatggctg attcagcgct
      301 accgcagaca cggatacgag attaagaaag ccctcagtct cgattatcgt cactggataa
      361 tcttcgtcag gctcccttat tccgaacggc caccgcgtcc gtcctgcaca ttccagcaac
      421 ggatctggag gtaatgtgcg ggtattactt cgacctgttc tggttccgga actcgggctg
      481 gtggtcctta agcccggtcg tgaatccatg ccggtattcc acaatacccg ggtactggtg
      541 gagccggaac cgaaaagcat gcgtaatctg ccgtccgggg tcgttcctgc cgttcgccag
      601 ccgctagtgg aagacaaaac attgctgccg tttttcagta acgcacgggt gattcgtgct
      661 gctggtggtg ctggcgcatt gtctgactgg ctgttgcgcc atattaaatc ctgccagtgg
      721 ccacacggcg attatcatca cagcgaaacc gtcattcacc gttatggtac cggcgcaatg
      781 gtattgtgct ggcactgcga caaccagctg cgtgaccaga catccgaatc actcgagcaa
      841 cttgctcatc aaaacctgtc agcatggatg attgacgtca tcggtcacgc aataagcggt
      901 acgcaggagc gtgaattatc tctggctgaa ttatcctggt gggcggtccg caatcaggtg
      961 gcggacgcgc taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc ggaaaaaatc
     1021 cgctcaatgt accgtgaaag cgacatcgtg ccgggagagc agaccgccac cagcatactg
     1081 aagcagcgca caaaaaatct tgcgccgctg cctcacgccc accagcaaca gaatccacca
     1141 caggaagaga cggtggtcag cattgccgtt gatccggaat ctccggctca gtatctccag
     1201 cgccagaaac cacaacggga agagatgcct gtatacacgc gctgggtaaa aacgcagaaa
     1261 tgcatgacgt gtggcaatca ggcagatgat ccgcaccaca tcattggtca tggactggga
     1321 gggatgggaa caaaggctga cgatttgttt gttattccgc tgtgtcgtaa atgccataac
     1381 gaactacacg ccggggtaaa agattttgaa gaaaaacacg gtagtcagtt gttgttgctg
     1441 attcgttttt taatgcacgc gagaaattcg ggtgttctga agtggaaagc ataaatgacc
     1501 gaacgcatag aatttgtttt gccttacccg ccaacggtga acacctactg gcgacgtcgt
     1561 ggcagcacat attttgtatc aaaagccggt gagcgttatc gccgtgatgt ggcgctaatt
     1621 gttcgccagc agcgactgaa attaaacctg tccggaaggc tggcgataaa gattattgca
     1681 gagccaccgg ataagcgccg tcgtgacctg gacaatatcc tgaaggcacc gctggatgcg
     1741 ctgacgcatg ctggactact tatagacgac gagcagtttg atgaaatcaa tattgtgtgc
     1801 ggtcagctcg ttcctggtgg gcggctgggg ataaaaatca cagaactggg gtgcgcatga
     1861 ataaccagta tttacagttt gtgcgtgagc agctcattat cgccaccgct gatttgagtg
     1921 gggcaacaaa aggtcagctt gaagcctggc aagagaatgc catgttcgat acagggcgtt
     1981 acaggcgtaa aaaaatccgg taccgcgatg aagtgactgg aaaaatgata acgcgggata
     2041 atccaccaat cccgggaaag caatcgctgg cgaaggggac gtcaattcct ctggtcagtc
     2101 cggttgagtt ttcgacatca tcgtggcggc gggctgttct gtctcttgaa gagcactata
     2161 aagcctggtt gttgtggtgt tacagcggga gtatttgttg ggaatatcag atcgcgataa
     2221 cacagtgggc gtggaatgaa tttaatgctc aatccggtac cagaaaaatt gcagggaaaa
     2281 cgcaggaacg cctgaaaaaa ttaatctggc tggcggcgca ggcagtaaaa gcagaacttt
     2341 ttggtgggga aggttatgaa taccaggagc tggcattact ggcgggagtg acaactaaaa
     2401 actggtccaa aacatttact cgtcactggg ttgcaatgaa acacattttt caccgactgg
     2461 atagtgaggc tttattgttt gtaatgagaa cacgttcaaa aaaaagacgg cattttcaaa
     2521 gcaaagtgtt gcaaaagtag attgaaaggc atatattcat gcaaatctga tactttgccg
     2581 attttgtacg tgatggcaaa agcaaacaaa acccgcccac aagcgggttt tttgtgccac
     2641 ttatctcgga tagacatggt gaatgcgctg gtggaggaga taagggtgat ttttgaatgc
     2701 ttgcaacatt gatttcgtaa cgttattatc ctgcgcccgg ccctttagct cagtggtgag
     2761 agcgagcgac tcataatcgc caggtcgctg gttcaaatcc agcaagggcc accaaccgcc
     2821 actagctcat caggaaagag cgtcaaccct ttaagttgag tgtgcgaggt tcgagtcccc
     2881 ggtggcggtc cagtgccgac ttggctcagt aggtagagca actgacttgt aatcagtagg
     2941 tcaccagttc gattccggta gtcggcacca tatgcgggca tcgtataatg gctattacct
     3001 cagccttcca agctgatgat gcgggttcga ttcccgctgc ccgctccagt cagagtcttt
     3061 cagtctgcga tgatgggaaa tcccggagtg actgaaagac gtttaagtta tgaatgatcg
     3121 cttttttttg caaaattgct gtgcagaaat actaaccttc gggcaggcga tcattcataa
     3181 gcactctgct tttattccga ttaactgtga gtggtttgtt ggatagagtg ctttccttac
     3241 tgtatatatc gtttcgcccg cttttgcggt tttttctttt caaatccctt tcatttctca
     3301 gtgtaaaact acgccatccg ttatttgcgg aggtgaggct atgaaatcca tggacaaaat
     3361 ttcaacgggc attgcctacg gcacctccgc aggcagtgct ggctactggt ttttacagtg
     3421 gcttgatcag gtcagtccgt cacagtgggc tgcgattggt gtactgggga gtctggttct
     3481 gggcttcctg acttatctga caaatctgta cttcaaaatc agagaagaca agcgtaaggc
     3541 tgcgagaggt gaataatgtc gccatcatta
//
LOCUS       DBKELA010000208         3548 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.212, whole genome shotgun sequence.
ACCESSION   DBKELA010000208 DBKELA010000000
VERSION     DBKELA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.212"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACUQR0_004067"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACUQR0_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HHV9110870.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            563..637
                     /locus_tag="ACUQR0_004068"
                     /pseudo
     CDS             563..637
                     /locus_tag="ACUQR0_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACUQR0_004069"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACUQR0_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HHV9110871.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACUQR0_004070"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACUQR0_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HHV9110872.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DBKELA010000209         3373 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.213, whole genome shotgun sequence.
ACCESSION   DBKELA010000209 DBKELA010000000
VERSION     DBKELA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.213"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(394..585)
                     /locus_tag="ACUQR0_004071"
     CDS             complement(394..585)
                     /locus_tag="ACUQR0_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HHV9110873.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDH
                     ADELYRSCLARFGDDGESN"
     gene            649..972
                     /locus_tag="ACUQR0_004072"
     CDS             649..972
                     /locus_tag="ACUQR0_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HHV9110874.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPT
                     LSPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQG
                     VRDDS"
     gene            962..1279
                     /locus_tag="ACUQR0_004073"
                     /pseudo
     CDS             962..1279
                     /locus_tag="ACUQR0_004073"
                     /inference="COORDINATES: protein motif:HMM:NF015397.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxase MbeA"
     gene            1650..2210
                     /locus_tag="ACUQR0_004074"
     CDS             1650..2210
                     /locus_tag="ACUQR0_004074"
                     /inference="COORDINATES: protein motif:HMM:NF016710.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HHV9110875.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLN
                     RQKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPAL
                     TALSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKF
                     LVLPDGVKGENTWTVGDKNAVRLVRE"
     gene            2217..2450
                     /locus_tag="ACUQR0_004075"
     CDS             2217..2450
                     /locus_tag="ACUQR0_004075"
                     /inference="COORDINATES: protein motif:HMM:NF016769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HHV9110876.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNN
                     AILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
BASE COUNT          818 a          813 c         1046 g          696 t
ORIGIN      
        1 aaaaaaaaaa aacggatctg catcacaacg agagtgatcc caccgctcgc cgcagcccca
       61 gcgaccgaac gtagtgagcg agtgggcgag gaagcggaag agtcacagat gcaagcattg
      121 tgcacttacg cgcctgtgcc agtgtccgcg tgtgtgctgt ttatcgggtg cgctgttttt
      181 acatctgtgc ggctttttac tcctgacatg gtgcacttcc catccatgtc tgcaccgatg
      241 caaaatgaga taagccagta tacacatcgc tatcgctctg tgaactgggt cagggctgcg
      301 ccccgacacc cgcactgacg cgccctggcg ggcttgtctg ctcccggcat ccgctcacag
      361 gcaagctgtg aacgtctccg ggagctgcat gtttcagttg ctttcaccgt catcgccgaa
      421 gcgtgcgagg caactgcggt agagttcgtc ggcgtggtcg tgaagtgatt cgcagatgtc
      481 tgcctgttcg tccgcgtcca gatcgttgag tttttccagc agggttagcg tctggcttct
      541 gataaagcga gccatgttaa gggctgtttt ttcctgtttg gtcatggtct taaggctcct
      601 tgtatggggg atttgttagg gggaatgata ccgatgaaac gcgagaagat gctcacgata
      661 cgggttaccg atgaggagca cgcccggtta ctggaacgct gcgagggtaa acggctggcg
      721 gaatggatgc ggcgggtctg tctgggggag ccggttgcac gcacaggaaa actgccgaca
      781 ctatcgccgc cattgctgcg gcatctggct gccatcggga acaacctgaa ccagacagcc
      841 cggaaggtga acagcggcca gtggtcttcg attgaccggg ttcatgtggt ggccgcactg
      901 atggccatcg agggtgaatt gcgccagttg cggcaggctg tcagggaaca gggggtacgg
      961 gatgatagtt aaatttcatg ccagggggaa aggcggtgga agtggcccgg tcgattacct
     1021 gctggggcgg gagcgtaacc gcgaaggcgc aacggtgctc cggggtaacc cggaagaagt
     1081 gcgggaactc attgattcca cgccattctc gaagaaatac acttcaggcg ttctgtcgtt
     1141 cgcggagaag gagttaccgc cgggtgaacg cgaaagagtg atgacgagct ttgagcgtgt
     1201 tcttatgccc ggtctcgaaa aagaccagta cagcattctc tgggtggaac accaggataa
     1261 ggggcggctc gaactgaatt ttgtcatccc gaacatggag ctacagagcg gtaagcgcct
     1321 ccagccgtac tacgaccgcg cggacaggcc gaggataaat gcctggcaga cgctggttaa
     1381 tcatcattac gggctgcgtg acccgaacgc accggagaac agacggacgc ttgtcacgcc
     1441 aaataatctg ccaaaagcga aacaggaggc cgcagaagcg attacacggg gtcttgaggc
     1501 tctttaccat gccggagcaa taaaaacgcg tcaggatgtc acagaggcgc ttacagcggc
     1561 aggttttgag gtggtgagga caaccaaaag cagcatcagt attgccgacc cggagggggg
     1621 gcggaatctc aggttaaagg gggcgctgta tgagcaatct tttacaaatg ggaacagact
     1681 tcgagaagaa actgaaagag cgagccgctt ctaccgagaa catgctgaac agcgagttca
     1741 ggaagctcga agagtctgta gacaaggcat tgagcttaaa caggcagaaa atcagagacg
     1801 ctatcagcga acatacaaca tccgtgaaaa agcaactgga tacgctgagc accaccgtga
     1861 gcatgcagtt cagcaccaca gaggccgaac tgtcccggca gcagaaaaaa ctgctctggc
     1921 gggtgatcaa agggcggata ctgtttccgg cgctgaccgc gctcagcgtc acgggcggca
     1981 ttttcctggg gtgctggggg ctgatggagt ggcaggaaag caagattgcg aaaaatatcc
     2041 tgactatcag agagcaggaa aacacgctgg cgaaactcga ggcaaaaacc tggggggtga
     2101 cgttcgtcaa tggcgagaac gggaaatttc tggtgttgcc ggacggggtg aaaggggaaa
     2161 acacctggac ggtgggcgac aagaacgccg tgaggctggt cagggagtaa acgagtatga
     2221 cggaactgga agggcatttg ctgaacgcct tagagcattt gcaacaggac tatatgcggc
     2281 ggctgaacga gtgggagagc gccttcgcgg aattgcagaa gatgcacgag gttacgcaac
     2341 ggaacaacgc gatactgaac gagcgcgtcg tgatattgag tcagcaggtg cagagattag
     2401 ccgggcaagt ggatcgcttg agtcggttat ttatcgcgaa caacagctaa gggatgaaca
     2461 ggagcgtgcc cggtacagac atgaggtaca ggaacgcgcg gcacgggttc ggagtgggcc
     2521 atcaatggag atgtgaccgg ggaagtgacg aaatcttgcc attttgacca gttcgacggc
     2581 tggggcgagc gaaacaaatc gtgtgccgta tttgtgcata gctatgcaac atcctgaaaa
     2641 cgcatttcac gcgtttttgg ggattcagcc aggggaaaac atggtttttt tactttttga
     2701 gcgtaaagac gtgaaaaatc tcgttattgc acttgcgatc tttcgtcggc gggcgtagaa
     2761 cgaaaacagc tcgccaggaa ggcgagctgt ttcgggttca ttgaggtctc tgacctcctc
     2821 aatgttcctg tggaaactgt tgagatccac aacagaaacc agttcattca gtttgcatga
     2881 gccttctgta ttcttcaaga atttttcaac agcacagtct cccaggaacc ccgaaaaaaa
     2941 cctgtttttg cagatacttg cgtatttgcg agaatcgttt gcggttgggg gagctgtgac
     3001 gacggcgacc gcacgcgtga gagttccgct accggcgcag agagtttcat cgacagcgga
     3061 cacctgatga gttgccacaa aaaccgtatg tagcgcggct agattggttg agttagacct
     3121 tggcggcagt tcccggtatg tggtggcgtg acgggtgaga tggtgcggcg aagacgcata
     3181 cgtcacgcag gcgcgggttc ttccactgac gcgccccgtg atcgatggcc ttggctccgt
     3241 ccggcatatc gagagcggca aactacccgt cccttcactg ggaggggtga gttgttgccg
     3301 ttccctccaa cctttcacca tccggcatat cgcacgatcc catgtgcacc gcaactcctg
     3361 ataactccag tgc
//
LOCUS       DBKELA010000210         3201 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.214, whole genome shotgun sequence.
ACCESSION   DBKELA010000210 DBKELA010000000
VERSION     DBKELA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.214"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            102..3005
                     /locus_tag="ACUQR0_004076"
     rRNA            102..3005
                     /locus_tag="ACUQR0_004076"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3201
                     /gene="rrf"
                     /locus_tag="ACUQR0_004077"
     rRNA            3101..>3201
                     /gene="rrf"
                     /locus_tag="ACUQR0_004077"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          701 c          992 g          657 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc catgcgagag t
//
LOCUS       DBKELA010000211         3180 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.216, whole genome shotgun sequence.
ACCESSION   DBKELA010000211 DBKELA010000000
VERSION     DBKELA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.216"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            173..1276
                     /gene="proB"
                     /locus_tag="ACUQR0_004078"
     CDS             173..1276
                     /gene="proB"
                     /locus_tag="ACUQR0_004078"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HHV9110877.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1288..2541
                     /gene="proA"
                     /locus_tag="ACUQR0_004079"
     CDS             1288..2541
                     /gene="proA"
                     /locus_tag="ACUQR0_004079"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HHV9110878.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2656..2731
                     /locus_tag="ACUQR0_004080"
     tRNA            2656..2731
                     /locus_tag="ACUQR0_004080"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Thr,seq:cgt)
     gene            complement(2746..>3180)
                     /locus_tag="ACUQR0_004081"
     CDS             complement(2746..>3180)
                     /locus_tag="ACUQR0_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9110879.1"
                     /translation="NRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          753 a          805 c          878 g          744 t
ORIGIN      
        1 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
       61 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
      121 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
      181 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
      241 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
      301 tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
      361 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
      421 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
      481 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
      541 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      601 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      661 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      721 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      781 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      841 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      901 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      961 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     1021 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     1081 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     1141 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     1201 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     1261 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     1321 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
     1381 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
     1441 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1501 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1561 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1621 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1681 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1741 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1801 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1861 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1921 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1981 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     2041 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     2101 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     2161 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     2221 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     2281 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     2341 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     2401 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     2461 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2521 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2581 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2641 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2701 gtcgtaggtt cgactcctat tatcggcacc attaaaatca ataagttaca catcattagt
     2761 accttcctta ttttttgact gggacaaatt tgggaccgat gggttcagga tcgagtctat
     2821 ttgccgtgcg tgttcggtaa ggtgattagg tgcaaggtga gcatatcgac gaaccatttc
     2881 gatagactcc cagcctccca tttcctgtaa cactgacaac gggactccgg cttgaaccag
     2941 ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa tcatcaatac cagcccgtct
     3001 cagcgccgct ttccaggctg tgtttgcgtc ataccgcatc ttccttactg ttggcgcttt
     3061 cgttccgtct ggtttggtac agctttcctt gtacacaaat acccaacggt gatgattccc
     3121 gatttgtttt ttcaatacgc gacatgcagt atcattcagc gcaacgccaa ttgcgcggtt
//
LOCUS       DBKELA010000212         3178 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.217, whole genome shotgun sequence.
ACCESSION   DBKELA010000212 DBKELA010000000
VERSION     DBKELA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.217"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            417..1526
                     /gene="wzz(fepE)"
                     /locus_tag="ACUQR0_004082"
     CDS             417..1526
                     /gene="wzz(fepE)"
                     /locus_tag="ACUQR0_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HHV9110880.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1771..1905)
                     /locus_tag="ACUQR0_004083"
     CDS             complement(1771..1905)
                     /locus_tag="ACUQR0_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHV9110881.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2551..2721)
                     /locus_tag="ACUQR0_004084"
     CDS             complement(2551..2721)
                     /locus_tag="ACUQR0_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HHV9110882.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          800 a          658 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgtccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtggtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg gggggaccct gagcaggtgg taattgtaag acgttgcggg
     2821 cgatggtatt acaattgcac atcctgtcct gtttctttag gacgtatcat ggcacagcat
     2881 atcaactgat aaataattcc tgtgcagtgc agccgctgtt gcataaaatt tcgctctgcg
     2941 tcgagtgttt cttaatcaat aattccgctg atactccgga aaaacgcgag tctgttctca
     3001 tgcctgaaat gcccacaccc cacgcaaaaa caagtttttg ctgatttttc tttataaata
     3061 gagagttatg acaaattagt tcttcttgct ctctttgtga tatttaaaaa agcggtgtcg
     3121 gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt ggtactgact attttcat
//
LOCUS       DBKELA010000213         3019 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.218, whole genome shotgun sequence.
ACCESSION   DBKELA010000213 DBKELA010000000
VERSION     DBKELA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.218"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="ACUQR0_004085"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="ACUQR0_004085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HHV9110883.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>3019
                     /locus_tag="ACUQR0_004086"
     CDS             1647..>3019
                     /locus_tag="ACUQR0_004086"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - gamma-aminobutyrate shunt
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HHV9110884.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIA"
BASE COUNT          764 a          698 c          800 g          757 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agcgatcacc
     3001 cgaaactgca gggtattgc
//
LOCUS       DBKELA010000214         3018 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.219, whole genome shotgun sequence.
ACCESSION   DBKELA010000214 DBKELA010000000
VERSION     DBKELA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.219"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            494..1660
                     /gene="nhaA"
                     /locus_tag="ACUQR0_004087"
     CDS             494..1660
                     /gene="nhaA"
                     /locus_tag="ACUQR0_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HHV9110885.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1726..2625
                     /gene="nhaR"
                     /locus_tag="ACUQR0_004088"
     CDS             1726..2625
                     /gene="nhaR"
                     /locus_tag="ACUQR0_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HHV9110886.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2663..>3018)
                     /locus_tag="ACUQR0_004089"
     CDS             complement(2663..>3018)
                     /locus_tag="ACUQR0_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HHV9110887.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          708 a          670 c          740 g          900 t
ORIGIN      
        1 ggggcttttc tctatctgcc gttcagctaa tgcctgagac agacagcctc aagcacccgc
       61 cgctattata tcgctctctt taacccattt tgttttatcg attctaatcc tgaagacgcc
      121 tcgcattttt gtggcgtaat tttttaatga tttaattatt taactttaat ttatctcttc
      181 atcgcaatta ttgacgacaa gctggattat ttttgaaata ttggcctaac aagcatcgcc
      241 gactgacaac aaattaatta ttacttttcc taattaatcc ctcaggaatc ctcaccttaa
      301 gctatgatta tctgggctta gggtcactcg tgagcgctta cagccgtcaa aaacgcatct
      361 caccgctgat ggcgcaaatt cttcaatggc tcgtaaaaaa cgaattattc ctacactata
      421 atctgatttt aacgatgatt cgtgcggggt aaaatagtaa aaacgatcta ttcacctgaa
      481 agagaaataa aaagtgaaac atctgcatcg attctttagc agtgatgcct cgggaggcat
      541 tattcttatc attgccgcta tcctggcgat gattatggcc aacagcggcg caaccagtgg
      601 atggtatcac gactttctgg agacgccggt tcagctccgg gttggttcac tcgaaatcaa
      661 caaaaacatg ctgttatgga taaatgacgc gctgatggcg gtatttttcc tgttagtcgg
      721 tctggaagtt aaacgtgaac tgatgcaagg atcgctagcc agcttacgcc aggccgcatt
      781 tccagttatc gccgctattg gtgggatgat tgtgccggca ttactctatc tggcttttaa
      841 ctatgccgat ccgattaccc gcgaagggtg ggcgatcccg gcggctactg acattgcttt
      901 tgcacttggt gtactggcgc tgttgggaag tcgtgttccg ttagtgctga agatcttttt
      961 gatggctctg gctattatcg acgatcttgg ggccatcatt atcatcgcat tgttctacac
     1021 taatgactta tcgatggcct ctcttggcgt cgcggctgta gcaattgcgg tactcgcggt
     1081 attgaatctg tgtggtgtac gccgcacggg cgtctatatt cttgttggcg tggtgttgtg
     1141 gactgcggtg ttgaaatcgg gggttcacgc aactctggcg ggggtaattg tcggcttctt
     1201 tattcctttg aaagagaagc atgggcgttc tccagcgaag cgactggagc atgtgttgca
     1261 cccgtgggtg gcgtatctga ttttgccgct gtttgcattt gctaatgctg gcgtttcact
     1321 gcaaggcgtc acgctggatg gcttgacctc cattctgcca ttggggatca tcgctggctt
     1381 gctgattggc aaaccgctgg ggattagtct gttctgctgg ttggcgctgc gtttgaaact
     1441 ggcgcatctg cctgagggaa cgacttatca gcaaattatg gtggtgggga tcctgtgcgg
     1501 tatcggtttt actatgtcta tctttattgc cagcctggcc tttggtagcg tagatccaga
     1561 actgattaac tgggcgaaac tcggtatcct ggtcggttct atctcttcgg cggtaattgg
     1621 atacagctgg ttacgcgttc gtttgcgtcc atcagtttga caggacggtt taccggggag
     1681 ccataaacgg ctcccttttc attgttatca gggagagaaa tgagcatgtc tcatatcaat
     1741 tacaaccact tgtattactt ctggcatgtc tataaagaag gttccgtggt tggcgcagcg
     1801 gaggcgcttt atttaactcc acaaaccatt accggacaga ttcgagcgct ggaagagcgc
     1861 ctgcaaggca aattatttaa acgcaaggga cgtggtctcg aacccagcga gctgggagaa
     1921 ctggtctatc gctatgccga taaaatgttc accttaagcc aggaaatgct ggatattgtg
     1981 aactatcgca aagaatccaa tttattgttt gacgttggcg tggctgatgc actttccaaa
     2041 cgcctggtca gtagcgtact taacgccgca gtggtagaag gcgagcccat tcatcttcgc
     2101 tgcttcgaat ccacccacga aatgctgctg gagcaattaa gtcagcataa actggatatg
     2161 atcatttctg actgtccgat agactctacg cagcaggaag gcctgttctc cgtgagaatt
     2221 ggcgaatgtg gcgtgagttt ctggtgtaca aatccaccac cagaaaaacc gttcccggct
     2281 tgtctggaag aacggcgact tttgattcct gggcgacgtt caatgttagg gcgcaaattg
     2341 cttaactggt ttaactccca gggattaaac gtagaaatcc tcggcgagtt tgatgatgcc
     2401 gctttgatga aagcttttgg tgcgatgcac aatgcaatct tcgttgcccc aacgctttat
     2461 gcatatgact tttatgccga taaaactgtc gtagaaattg gtcgcgtcga gaatgtgatg
     2521 gaagagtacc atgctatttt tgctgagcgg atgattcagc acccggcggt acagcgaatc
     2581 tgcaatacgg attattctgc gctttttagt ccagcggtgc gttaatcggc agctccccca
     2641 aagttaaggt gggggagata gattagttgt acattaccac gattttgact cggctcatta
     2701 tttgcccgct tgagacattg tttccatatg tacgcgggcg aataaataga ggaatctgat
     2761 tacttccttc atggggatgc tgaaaagagt agtaattgct tttaccatac tggatttcat
     2821 tcgtaccatc attggagagt tgaaagccta tttgacccgc accgtcctca ctgttttcta
     2881 actcattttt taatatgctg gttcctgact cctgtatcgt cactggttca aagtaatatt
     2941 caacgtcact gggttcaaaa actaattcac cttgctcgcc ctggatgcag tcaaatctta
     3001 tatgatgaaa ctgataat
//
LOCUS       DBKELA010000215         2949 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.220, whole genome shotgun sequence.
ACCESSION   DBKELA010000215 DBKELA010000000
VERSION     DBKELA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.220"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            139..2007
                     /gene="kup"
                     /locus_tag="ACUQR0_004090"
     CDS             139..2007
                     /gene="kup"
                     /locus_tag="ACUQR0_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HHV9110888.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2174..2593
                     /gene="rbsD"
                     /locus_tag="ACUQR0_004091"
     CDS             2174..2593
                     /gene="rbsD"
                     /locus_tag="ACUQR0_004091"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HHV9110889.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2601..>2949
                     /locus_tag="ACUQR0_004092"
     CDS             2601..>2949
                     /locus_tag="ACUQR0_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHV9110890.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          656 a          702 c          779 g          812 t
ORIGIN      
        1 gggggggctt tttctttgag ccaggttaat gtggccgcat ttaggagtac gattttgccg
       61 ttaatcgtgc atactgtgcg cttttttgtg ggccaaggga ctaagcacac atttcatatt
      121 tcaacgaaag actagtctat gagcactgat aataagcaat cattgcccgc gattaccctc
      181 gcggcgattg gagttgtcta cggcgatatt ggtaccagcc cgttatatac acttcgtgaa
      241 tgtttgtccg gccagtttgg ttttggcgtt gaacgcgatg ccgtgtttgg ctttttatcg
      301 ctgatcttct ggctgctaat ctttgtggtt tccattaaat atctcacctt cgtgatgcgg
      361 gcagataacg ccggtgaggg ggggatcctg acgttgatgt cgcttgccgg gcgtaatacg
      421 tcggcgcgaa ccacatcaat gctggtgatt atggggctaa tcggcggcag ctttttctat
      481 ggtgaagtcg tcataacacc cgctatttcg gtgatgtcag ccattgaagg tctggaaatc
      541 gtcgccccgc agctggatac ctggatagtt cccctctcaa ttatcgttct cactttatta
      601 tttatgattc aaaagcatgg cacggctatg gtcggtaagc tgtttgcgcc gatcatgctg
      661 acctggtttt tgattctggc aggactgggg ttacgtagca ttattgctaa cccggaagtg
      721 ctgcatgcgc tgaatccgat gtgggcggtg catttcttcc ttgaatacaa aacggtttct
      781 tttattgcat taggggcagt ggtgctgtcg attacggggg gcgaggtgct gtatgctgat
      841 atggggcact ttggtaagtt ccctattcgc ctggcgtggt tcaccgtcgt attaccttcc
      901 ttgaccctta attacttcgg tcagggagcg ttgttgttaa agaacccgga agcgattaag
      961 aacccgttct tcctgttggc accggactgg gcgctgatcc cactgctgat catcgccgca
     1021 ctggcgacgg taattgcctc gcaggcggtt atctctggcg tcttctcatt gacgcgtcag
     1081 gcggtacgtc tgggatattt gtcgccgatg cgcattattc acacctccga aatggagtca
     1141 gggcaaatct atattccgtt tgtgaactgg atgctctatg tcgcggtcgt gattgtgatt
     1201 gtcagctttg agcactccag caacctggcg gcggcgtacg ggattgcggt gaccggaacc
     1261 atggtactga cgtctattct ctcgactacc gtggcacgtc agaactggca ctggaataag
     1321 tattttgttg cgctgatcct gattgctttc ctttgtgtcg atattccatt gttcaccgct
     1381 aacctcgata aactgctctc cggcggctgg ttgccattga gcctcggtac tgtgatgttt
     1441 atcgtgatga ccacctggaa gagcgagcgt ttccgcttgc tgcggcggat gcatgaacat
     1501 ggtaactctc tggaagcgat gattgcttcg ctggagaaat caccgcctgt tcgcgtgccc
     1561 gggaccgcgg tgtatatgtc gcgtgcaatc aacgtcattc cctttgcgct gatgcataac
     1621 cttaaacata acaaggtatt gcatgagcgg gtgattctgt taactctgcg caccgaagac
     1681 gcgccatatg tccataacgt ccgtcgggta cagattgaac aactgtcgcc cactttctgg
     1741 cgcgtggtgg caagttatgg ttggcgagaa acgccaaacg tggaagaagt tttccaccgc
     1801 tgcggtctgg aaggattaag ttgccggatg atggaaacct ccttctttat gtcgcatgag
     1861 tcgttgatcc tcggcaaacg cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg
     1921 ctgcaacgta atgcgctgcg tgcaccagat caatttgaaa tcccgccaaa cagggttatc
     1981 gaactgggta ctcaggtcga aatctaacgc cagacgcctc ctttcttcat aagggggcgt
     2041 ttttgttttc atggttaatc accatgtaaa acgtttcgag gttgatcaca tttccgtaac
     2101 gtcacgatgg ttttcccaac tcagtcagga ttaagctgcg gatcagcgaa acgtttcgct
     2161 gatggagaaa aaaatgaaaa aaggcaccgt tcttaattct gatatttcat cggtgatctc
     2221 ccgtctggga cataccgata cgctggtggt gtgtgatgct ggtttaccca tccccaaaag
     2281 tacaacgcgt atcgatatgg cattaaccca aggtgtacct tcttttatgc aggtgctggg
     2341 cgtcgtcaca aatgaaatgc aggtcgaggc tgccattatc gcggaagaga tcaaacaaca
     2401 taatccgcaa ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa aacaccaggg
     2461 aaataccatt gaaattcgtt acaccacgca tgaacaattc aaacaacaaa ccgcagaaag
     2521 tcaggcggta attcgcagcg gagaatgttc tccgtatgcg aatatcattc tctgtgctgg
     2581 cgtgacgttc tgaggccgtc atggaagcat tacttcagct taaaggcatc gataaagcct
     2641 tcccgggcgt aaaagccctc tcgggcgcag cgttaaatgt ctatccgggc cgtgtgatgg
     2701 cgctggtggg cgaaaacggc gcgggtaaat ccaccatgat gaaagtgctt actggtatct
     2761 atactcgcga tgccggcacg cttttatggc tggggaaaga aacgacattt accgggccga
     2821 aatcttccca ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt
     2881 tgaccattgc cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg
     2941 actggaaaa
//
LOCUS       DBKELA010000216         2887 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.221, whole genome shotgun sequence.
ACCESSION   DBKELA010000216 DBKELA010000000
VERSION     DBKELA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.221"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..838)
                     /locus_tag="ACUQR0_004093"
     CDS             complement(<1..838)
                     /locus_tag="ACUQR0_004093"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HHV9110891.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFA"
     gene            1093..2322
                     /locus_tag="ACUQR0_004094"
                     /pseudo
     CDS             1093..2322
                     /locus_tag="ACUQR0_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            2749..>2887
                     /locus_tag="ACUQR0_004095"
     CDS             2749..>2887
                     /locus_tag="ACUQR0_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHV9110892.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT          780 a          668 c          589 g          850 t
ORIGIN      
        1 tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa cgctcgtgct ttggcttcgc
       61 tcatcatcat taccgcagct gcgccatcgt ttatggatga tgcattcccc gcggtcacag
      121 aaccgagact atcaaatgaa ggatttaaac gggctaaacc ttctgcactg gcgtcagtgc
      181 gtggctgttc atcggtatca acaaccaacg tctgaccgtt actttggatc attaccggga
      241 cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg cgctttttgt tgcgagctaa
      301 gtgcgtaagc atcctgcaac tgacggctga tgccatattc gcgagccaga ttttcggcgg
      361 tgacaccaat atgataatca ttgaaggcat cccacaaccc atcatgcacc agactgtcaa
      421 ccaactggct attgccaagc tgtgcaccgg tgcggctatc agtcagaaca tgtggtgcgc
      481 ggctcatgtt ttcctggcca ccggcgatga caatatcagc ctcgccacac tgtatcgcct
      541 gagtagccag atgcagtgct ttaagaccgg aaccgcaaac gtcattaatc gtgattgcag
      601 aaacgctgtt aggaagacca cctttaatag ccgattgcct tgccggattc tgccctgccc
      661 ctgcagtcaa cacctgacca agaattactt catccaccgc atatgcagga acgccggtac
      721 gttctattaa cgctttcacg accagactac caagttccac ggcggaatga cccgctaacg
      781 caccacgaaa gcagccgata ggtgtccgta acgccccgac aatcacaacg tctttcatca
      841 caacctcgca ccaattaaca gtgcaatagt aaatgattgt taattacagt tatcttaatt
      901 gtttaaaaaa agtgattttt atcacaaagg aaatatgcct gagcagtagt cagagtcata
      961 actggcacgt aaggtttgca accactaacc caccaataga tgggtagata gggcgttaat
     1021 ctcccatact taacttggtt tatggtaaat tgccctccat tttgtttaat ttgtagatga
     1081 tacgttcaga taatgtctaa tatttggtct aaagaagaaa ctctgtgaag tttcgcgctc
     1141 tatggcacag ccgttgatgc aggcacgcta ttccttccta ttcagttagg ttcggcaggg
     1201 gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatattg gccacataaa
     1261 gccttatgcc agttcatcct ctcatcaaaa acatcagcag gtgaagggat aacgggcgcg
     1321 gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta cttcatcgcc
     1381 ttttttgtcg tcgtgttgat atatgcagtg gcaattacca actcacttac ggaacagctg
     1441 gcaaagcata tggttattga tcttcgcatc cgtatgttgg tgagtctggg tgttgtatta
     1501 attctgaatc tcatttttct gatgggacgt catgccacta ttcgggtaat gggatttttg
     1561 gtattcccat tgattgccta tttcttattt ctttccattt accttgtcgg tagttggcaa
     1621 cctgatctat taacaaccca ggtagagttc aatcagaata cccttcacca gatatggata
     1681 tcgattcccg tgatggtttt cgcctttagc catacgccca ttatttctac gtttgccata
     1741 gacagacgtg aaaaatatgg cgaacacgct atggataaat gcaaaaaaat tatgaaagtc
     1801 gcttatctca tcatctgcat aagtgtactg ttctttgtct ttagctgcct gctttctatt
     1861 ccatcttcgt atattgaagc ggctaaagaa gaaggggtca ccattttatc ggcgctttct
     1921 atgctgccga acgccccagc atggttgtca atttccggga ttattgtcgc agtagttgcg
     1981 atgtcgaaat cattcctggg tacgtacttt ggcgttattg aaggtgccac agaggtcgtc
     2041 aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg catttaacca cgcactatca
     2101 attatgttgg tatcgctgat taccttcatt gtttgttgca ttaacccgaa cgcgatttcg
     2161 atgatttacg cgatcagcgg cccgctcatt gccatgatac ttttcatcat gcctacgctg
     2221 tcaacgtatc tcatcccggc gcttaaaccc tggcgttcca tcggaaatct gattacgctg
     2281 atcgtgggta tcctgtgcgt atcggtaatg ttctttagct aatatccgtt gtggatggtt
     2341 caaaattgct acaattttga accattccat ttttcataat attcatttgt tattattccg
     2401 ccctatcact tctctcgtca tatcctagaa ttatcagtta gttaaacaca caaagagatc
     2461 tgaacatctt aaataagact atttaagatg cataacttag attcgcaaga tatcttctgg
     2521 tcattcaaaa acaatttgcg atatagatat tctcatacca tgagatagtt atctggacca
     2581 cgttcgacca tgcaagatta aatcagtgaa acataatcat atttaataac gagaaatgca
     2641 tttttaaata tcttaatcag aattatagca aatacagatt aatcgcaatt tttcccagca
     2701 aaattacaaa atcaaggtgt attgacatca tcaatcacca atgaaattat gcaattatat
     2761 acggataggg aggttcttaa catggggctt tgtagtcgtt ataaaagtct tacatgcaat
     2821 agttgctcaa tgcattgcca aataatgcca gaagagtcac cgcgtttgca gtactgcgca
     2881 aactcgt
//
LOCUS       DBKELA010000217         2807 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.222, whole genome shotgun sequence.
ACCESSION   DBKELA010000217 DBKELA010000000
VERSION     DBKELA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.222"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            6..81
                     /locus_tag="ACUQR0_004096"
     tRNA            6..81
                     /locus_tag="ACUQR0_004096"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACUQR0_004097"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACUQR0_004097"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACUQR0_004098"
     CDS             complement(299..1057)
                     /locus_tag="ACUQR0_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHV9110893.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACUQR0_004099"
     CDS             complement(1065..2168)
                     /locus_tag="ACUQR0_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHV9110894.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACUQR0_004100"
     CDS             complement(2178..>2807)
                     /locus_tag="ACUQR0_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHV9110895.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          738 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DBKELA010000218         2690 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.223, whole genome shotgun sequence.
ACCESSION   DBKELA010000218 DBKELA010000000
VERSION     DBKELA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.223"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            44..769
                     /locus_tag="ACUQR0_004101"
     CDS             44..769
                     /locus_tag="ACUQR0_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HHV9110896.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACUQR0_004102"
     CDS             complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACUQR0_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HHV9110897.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="ACUQR0_004103"
     CDS             1604..1798
                     /locus_tag="ACUQR0_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HHV9110898.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            2296..2457
                     /locus_tag="ACUQR0_004104"
     CDS             2296..2457
                     /locus_tag="ACUQR0_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHV9110899.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DBKELA010000219         2566 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.224, whole genome shotgun sequence.
ACCESSION   DBKELA010000219 DBKELA010000000
VERSION     DBKELA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.224"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..489
                     /gene="capU"
                     /locus_tag="ACUQR0_004105"
     CDS             <1..489
                     /gene="capU"
                     /locus_tag="ACUQR0_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HHV9110900.1"
                     /translation="EFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHD
                     DVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQN
                     NQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAK
                     QK"
     gene            494..1444
                     /gene="virK"
                     /locus_tag="ACUQR0_004106"
     CDS             494..1444
                     /gene="virK"
                     /locus_tag="ACUQR0_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HHV9110901.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1509..2453
                     /gene="lpxM"
                     /locus_tag="ACUQR0_004107"
                     /gene_synonym="msbB"
     CDS             1509..2453
                     /gene="lpxM"
                     /locus_tag="ACUQR0_004107"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HHV9110902.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          741 a          488 c          582 g          755 t
ORIGIN      
        1 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
       61 attgtggggt ctggttcacc tgaactgcgg gaacatttac agtatcagat tgacagtatg
      121 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
      181 gttgccagtc tggtggttct gccttcagaa aacgaatctt ttggtatggt gctggcagaa
      241 gcatcggcat tttctgtgcc tgtagtggcc actcagattg gtggaatccc tgaggttatt
      301 cagaacaacc agaccgggac attgttacca gcaagtaata agcacgcatg gatgtgcgcc
      361 ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcct ggcaaaacag
      421 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
      481 caaaagtaac aatatgtttt ctataagtaa cttatcattt atcggtttcc ttaaaaggat
      541 tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc ggttcatgta
      601 cattttgcga tgttctataa atccggttgt cagtattcga tattattatg aactgcgttc
      661 cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa gaattcatcg
      721 cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc ttgagcatta
      781 tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac aaaaatcggt
      841 atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt gttctcccag
      901 tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa cagttattgc
      961 ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta ttggcggatt
     1021 acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     1081 tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgcaa
     1141 tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     1201 gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg
     1261 aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc cgctaagcga
     1321 tatagccagt aaaaaacgct ctgagtaccg aaaacgatac gctttgctgg attatatcca
     1381 tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc agaatttaaa
     1441 ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag aggcattcgc
     1501 actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa aattaccttt
     1561 cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt tcaatgtttc
     1621 ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc agattgagca
     1681 aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa aagagtgata
     1741 cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc atagtgatga
     1801 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     1861 tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg gttccccatg
     1921 gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa atggccgcca
     1981 tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta cggcgtaaat
     2041 tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag tcagtacgcc
     2101 agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac agtgaatttg
     2161 ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg atgaacatca
     2221 gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat ctactgacta
     2281 ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa acgattgccc
     2341 gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag tatgtctggg
     2401 ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct tgaaattata
     2461 aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat
     2521 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaac
//
LOCUS       DBKELA010000220         2525 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.225, whole genome shotgun sequence.
ACCESSION   DBKELA010000220 DBKELA010000000
VERSION     DBKELA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.225"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..567
                     /locus_tag="ACUQR0_004108"
     CDS             <1..567
                     /locus_tag="ACUQR0_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9110903.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="ACUQR0_004109"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="ACUQR0_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HHV9110904.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACUQR0_004110"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACUQR0_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HHV9110905.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="ACUQR0_004111"
     CDS             2169..2405
                     /locus_tag="ACUQR0_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HHV9110906.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          647 a          563 c          650 g          665 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttatccag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgcagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2461 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
     2521 ccacc
//
LOCUS       DBKELA010000221         2442 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.226, whole genome shotgun sequence.
ACCESSION   DBKELA010000221 DBKELA010000000
VERSION     DBKELA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.226"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..333
                     /locus_tag="ACUQR0_004112"
     CDS             <1..333
                     /locus_tag="ACUQR0_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HHV9110907.1"
                     /translation="RDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIA
                     GGKKLPERETLEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAY
                     ELYLSEKQ"
     gene            357..647
                     /gene="lsrG"
                     /locus_tag="ACUQR0_004113"
     CDS             357..647
                     /gene="lsrG"
                     /locus_tag="ACUQR0_004113"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HHV9110908.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            686..1444
                     /gene="tam"
                     /locus_tag="ACUQR0_004114"
                     /pseudo
     CDS             686..1444
                     /gene="tam"
                     /locus_tag="ACUQR0_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
     gene            complement(1448..2362)
                     /locus_tag="ACUQR0_004115"
     CDS             complement(1448..2362)
                     /locus_tag="ACUQR0_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HHV9110909.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          652 a          574 c          635 g          581 t
ORIGIN      
        1 cgcgatcagc gttatttctc gctcgcgact cgaatcgccg ctgaaatggg ggcgcaaatt
       61 atcaaaacct attatgtcga aaaaggtttt gaacggattg ttgccggatg tccggtaccc
      121 attgttattg ctggcggtaa aaaattaccg gagcgcgaga cgctggaaat gtgctggcag
      181 gctatcgatc agggcgcttc tggtgtggat atggggcgta atattttcca gtctgaccat
      241 ccggtggcga tgatgaaagc cgtacaggcg gtggttcacc ataacgaaac ggctgatcgg
      301 gcatatgaac tctatctgag tgaaaaacag taactgcgga tttaaggaga agaattatgc
      361 acgtcacact ggttgaaatt aacgttcatg aagacaaggt tgacgagttt atcgaagttt
      421 ttcgccagaa ccacctgggc tctgtacagg aagaaggtaa tttgcgcttc gatgtcttac
      481 aggacccgga agtgaattcg cgcttttata tttacgaagc ctataaagat gaagacacag
      541 tggcattcca taaaaccacg cctcactaca aaacctgtgt cgcgaaactg gaatctttaa
      601 tgaccgggcc gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc tacaattggg
      661 gtaacgcgct gacgggagta aaaaaatgtc tgactggaac ccctctttat atctgcactt
      721 ttccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc agagtgccgc tggagaatgt
      781 cgaatatgtt gccgatcttg gctgtggccc aggtaacagc accgccctgc tgcatcaacg
      841 ttggcctgcg gccaggataa caggcatcga ctcgtctccg gcgatgattg ctgaagctcg
      901 cagtgctttg ccagactgcc agttcgtgga agcagacatc cgcaactggc aaccggaaca
      961 ggcactcgat ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt
     1021 gtttcctcac ttggtttctt tacttaatcc gcagggcgtg ctggcagtac agatgccaga
     1081 taactgactg gagccgaccc atgtgctcat gcgcgaagtt gcttgggaac aaaactaccc
     1141 ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac tacgatattt tgagcgaagc
     1201 cggatgtgag gtcgatatct ggcgaaccac ttactatcac cagatgccgt cgcaccaggc
     1261 gattatcgat tgggtgaccg ccactggatt acgtccgtgg ttacaggatc tgaccgagag
     1321 cgaacagcag ctttttctta cgcgctacca tcagatgctg gaagagcagt atccactgca
     1381 agagaacgga cagatactgc tggcatttcc gcgtctgttt attgttgccc gccgtacgga
     1441 gtaattatca cgtcagctgg taatgacgat cgggaagaat ttttgctgga atttctgctt
     1501 catcgttcat ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaaat
     1561 cattgttttc agtaccgaac ggttcttcca gttcttccgc cagacaatcc agcgaaataa
     1621 aagtgtagga aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg
     1681 cgaacggcag catgatacaa aacagataaa cggtacgatg caaaatcagg gtgtaggcaa
     1741 agggaattgg cgtgtaggca atgcgctcac agcctgccag aaccgctgaa atatcattaa
     1801 gccgatcgtt gaggctaata aacaggatat ctgaaagctg tccattgcgg cgctgaaccg
     1861 ccaaccattc tcccattatt aacaagatac ggttagctgg agagttcgaa gccagtacac
     1921 gctgaagatc tccagtcttg agataatgag ccagcacttc cgcctgtggc tgtttgcgta
     1981 atgtcatgcg taaacagtgg gcgaaggcga tttgcagcct ggcaaactcc cttacacttg
     2041 ccgaatccgg caatgtcgtt tttacctctc gcagtaacga ccgtgaggca atcatcaact
     2101 gcccccaaag ttttcgcgct tcaacgtaac gggcgtaccc ggcattatta cgaaaaccaa
     2161 gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttgatgccca
     2221 gatgcgtgta ccagggcagc atgaatataa cagcaataga aaagagaaaa ttgagtagta
     2281 agcgcgagga tatcttggat aatactgagc cgtgccagac aaaaatacgg cgcagccagt
     2341 gttgttgtgg acgaacaatc atggttatct tcaggcgtgg aaaagtcgcc ctattaaacg
     2401 tgattacgat cacattctca agacgctact tacaaattac ct
//
LOCUS       DBKELA010000222         2442 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.227, whole genome shotgun sequence.
ACCESSION   DBKELA010000222 DBKELA010000000
VERSION     DBKELA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.227"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..706
                     /locus_tag="ACUQR0_004116"
     CDS             <1..706
                     /locus_tag="ACUQR0_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110910.1"
                     /translation="CEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADL
                     AIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQ
                     PSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSS
                     VNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLS
                     GAGISSSFAFSITRLI"
     gene            complement(760..1932)
                     /locus_tag="ACUQR0_004117"
     CDS             complement(760..1932)
                     /locus_tag="ACUQR0_004117"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHV9110911.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2281..>2442)
                     /locus_tag="ACUQR0_004118"
     CDS             complement(2281..>2442)
                     /locus_tag="ACUQR0_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HHV9110912.1"
                     /translation="QEMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQS
                     RNKEGTNDV"
BASE COUNT          738 a          444 c          505 g          755 t
ORIGIN      
        1 ttgtgaagga aatggtactc agggggattc cagtacaggt ggtgcgcaat tagctataaa
       61 tggccttaac ggttcgtgcg ctttggtatt aaacaaccca tactttgagg ctaatgccgg
      121 gggggcagac cttgctatag ataatactgg gacgcgccct gtaactgtgg ttataaacgg
      181 tggaaatttt catcgcgtga gcagtgcaag atacacgcac acgaatattc aagtaacaag
      241 ctcaggtggg ggaaaagtta cagtcatact caacggcaca acgttccaga gcgcagggga
      301 ttatcagcca agtgcagaac gaccatactg gataacagga gctaattgcg aggtagttga
      361 cattgggtgt gtttttacag aaacaaccag taaggtaaca tcggcttctg cattgtcagt
      421 cacacgctcg ggaaaaatta acgccaatgg tagtatcgac gttgctactg gtgtttcatc
      481 ggttaacgtg gtttctacag gtgtgtatga tgttagcttt tctcatccgc tcgcagcagt
      541 tgctagcggt tatattgtgc agataacacc tatatctgcg cctgattcag ttagttgtga
      601 tgttacttat attggcgtag atacatttag ggtgaccctg cgcaatacgc ttagcggggc
      661 aggaatctcc agttcgtttg catttagcat taccagacta atataaatac attcgccgcc
      721 ccatttggac atatatgtag ggcggcttat gtttattgtt tattgattgt tgtttgttaa
      781 tgtgggtatt aattctttta ctaaagttgt ggtttgtttt gttaatttga tgaatggcgt
      841 ctctattaat ttaaaagtta ggcatgatgt gaacgtaata agaaatgcag ttgtgcttac
      901 cagtatgata aatgtatttt cagtgtaatt attaggaata atccacgtca ttaggcaata
      961 aaggaatatc ccatgcagaa ggtaaacgct gtaggtagtt tctcctagct ttctggttat
     1021 atttaatctt aatattccat acaaatcaca acctgatgcg ataatgataa atgttattcc
     1081 acaaagaatc agtggtagcg gtgcgtaagt tgttttgaaa tacgtcattt caaaaaccat
     1141 tatcgcagtt attataattg gtgttacttt tgctttggca attccattga ctatctttga
     1201 tttatttagc aaaaatgcca ggagcccaaa aagaaatgat gctatgtgta ttttactgaa
     1261 aaataaaatg aaaacaaata gcgatattgc gctaatcata accatgtaaa tactgacttt
     1321 tctttttatg agcgcggaaa ttaccggaag agaaaaataa aagaaccatt catatacaag
     1381 tgtccatgtt acagcggcat tgatagtaaa tgaatctttt acgtcattaa tgttcggcat
     1441 acctagcgca gtgaatggca accacttcat tattgacaca aaaagctctt ctgtggatac
     1501 ctgcattctc catccagact taaaacctac aatgataaaa atgagacata ggctaactat
     1561 gaacattggg gttaatcgta gtaatcttga tacataaagc cttgtccagt cctgatctcc
     1621 agagataatc tttgaaaaga acagataacc agtaatcata aaaaagaatg acacaccaac
     1681 ttggcctaaa tttgctaaca gatttgatga aggtgcttcc catactccag atgacaagta
     1741 tccattccaa attgctgcgt gatgaataag cacgaaaatt gctaagaacc cccgcattcc
     1801 atctatttga ttattcctgc cgtgctcaac aagatttatt ggggaaattt tatcaaataa
     1861 ccccacagct aataacgaca tcaatatcac ggatgttatt aataataaac tgttaatttc
     1921 aatcatatgc atgctctatt acgcaattaa aatttctcca agtatactga cttttagtta
     1981 atgatcatat agttatgtgt aactgatgaa acaaaacgga gacacaccaa gctttgcact
     2041 ggattgcaag gctttgtgct cttctgtgaa tgtgtggcta tatgtttgaa gatcgttgtg
     2101 ccgtatttgt gacatatata tggcaacatc atgcatcaac tttctgtttg tgtcatcaac
     2161 tatagcttag tgaatgcggt taatgcttgc taaaacagat agttatgatt ggtgctacag
     2221 attcgtaatg cgaaggtcgt aggttcgact gctattatcg gcaccattta aatcaataag
     2281 ttacacatca ttagtacctt ccttatttct tgactgggac aaatttggga ccgatgggtt
     2341 cagaatcaag tctatttgcc gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata
     2401 tcgacgaacc atttcgatag actcccagcc tcccatttcc tg
//
LOCUS       DBKELA010000223         2432 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.228, whole genome shotgun sequence.
ACCESSION   DBKELA010000223 DBKELA010000000
VERSION     DBKELA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.228"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            35..1543
                     /locus_tag="ACUQR0_004119"
                     /pseudo
     CDS             35..1543
                     /locus_tag="ACUQR0_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2432
                     /locus_tag="ACUQR0_004120"
     CDS             1540..>2432
                     /locus_tag="ACUQR0_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HHV9110913.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          629 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa ca
//
LOCUS       DBKELA010000224         2391 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.229, whole genome shotgun sequence.
ACCESSION   DBKELA010000224 DBKELA010000000
VERSION     DBKELA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.229"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..108
                     /locus_tag="ACUQR0_004121"
     CDS             <1..108
                     /locus_tag="ACUQR0_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HHV9110914.1"
                     /translation="LQTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            101..1060
                     /gene="ssuA"
                     /locus_tag="ACUQR0_004122"
     CDS             101..1060
                     /gene="ssuA"
                     /locus_tag="ACUQR0_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HHV9110915.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1057..2202
                     /gene="ssuD"
                     /locus_tag="ACUQR0_004123"
     CDS             1057..2202
                     /gene="ssuD"
                     /locus_tag="ACUQR0_004123"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HHV9110916.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2214..>2391
                     /locus_tag="ACUQR0_004124"
     CDS             2214..>2391
                     /locus_tag="ACUQR0_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110917.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          527 a          654 c          673 g          537 t
ORIGIN      
        1 ctgcaaaccc atcttgatac cgcgctagaa actttctggc aggcattgca ccgccgcgat
       61 gttcaggttc ctgaccttct gtctctgcga ggtaatgccc atgcgtaaca tcattaaact
      121 ggcgctggcg ggattgctta gcgtctctac ttttgcggtt gctgcagaat cctcgcctga
      181 agcgttacgt ataggctatc agaaaggcag tattggtatg gtgctggcaa aaagccacca
      241 gttactggaa aaacgctatc cgcaatcaaa aatctcctgg gtggagttcc ccgcgggtcc
      301 gcaaatgttg gaagcgttaa acgttggcag tattgatctc ggcagtaccg gggatattcc
      361 gccaatcttt gcccaggctg ccggggctga tttggtatat gtgggcgtcg agccaccgaa
      421 gcccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg tagccgatct
      481 taaaggtcac aaagttgcct ttcagaaagg ttccagttca cacaaccttt tactgcgtgc
      541 actgcgtcag gccggactta aattcaccga tatccagccc acttacctga cgcctgccga
      601 tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg atccctacta
      661 ctccgctgcg ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg atctcaatca
      721 aactggatcg ttttatctgg cagctcgtcc ctatgcagaa aaaaacggcg cttttattca
      781 gggcgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc gcgaacaaag
      841 catcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct cttacttaga
      901 tcatcgccct cctaccacca tcaaaccggt taacgccgag gttgccgcct tacagcagca
      961 aacggcagat ctgttttatg aaaaccgtct ggtgccgaaa aaagtcgata ttcgccagcg
     1021 catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat gttctggttt
     1081 ttaccgaccc acggtgacgg gcactatctg ggaacggaag aaggttcacg cccggttgat
     1141 cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggctatac cggtgtgctg
     1201 atcccaacgg ggcgatcgtg tgaagatgcg tggctggttg cagcgtcgat gatcccggtg
     1261 acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc tactgttgcc
     1321 gcccgccagg ccgccacgct tgaccgtctc tcaaatggtc gtgcgttgtt taatctggtc
     1381 acaggcagcg atccacaaga gctggcaggc gacggcgtgt tccttgatca tagcgagcgc
     1441 tacgaagcct cggcggaatt tacccaggtc tggcggcgct tattgctggg ggaaaccgtc
     1501 aatttcaacg gtaaacatat tcatgtgcgc ggagcaaaac tgctcttccc gccgattcaa
     1561 cagccttatc ctccgcttta ctttggcgga tcgtcagatg tcgcccagga gctggcggca
     1621 gaacaagttg atctctacct cacctggggc gaaccgccgg aacttgttaa agagaaaatc
     1681 gaacaagtgc gggcgaaagc tgccgcgtat gggcgcaaaa ttcgtttcgg tattcgtctg
     1741 catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg gttaatctcg
     1801 catcttgatg atgagactat cgccaaagca caagccgcat tcgcccggac ggattccgta
     1861 ggacaacagc gaatggcggc gctacataac ggcaagcgcg acaatctgga gatcagcccc
     1921 aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg gaacggcgct ggtgggcgat
     1981 ggtcctacgg tcgccgcgcg aatcaacgaa tacgccgcgc ttggcatcga cagctttgtg
     2041 ctttcgggct acccgcatct ggaagaagcg tatcgggttg gcgagttgct gttcccacat
     2101 ctggatgtcg ccattccgga aattccccag ccgcagccgc tgaatccgca aggcgaagcg
     2161 gtggcaaatg attttatccc ccgtaaagtc gcgcaaagct aaggaacacg atgatggcaa
     2221 cgccagtgaa gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg ggcatcgtgg
     2281 cggtgtggca actggcctcc tcggttggct ggctgtcgac gcgtattttg ccttcaccgg
     2341 aaggagtggt gacggcgttc tggacgctct ccgccagcgg cgaactgtgg c
//
LOCUS       DBKELA010000225         2372 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.230, whole genome shotgun sequence.
ACCESSION   DBKELA010000225 DBKELA010000000
VERSION     DBKELA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.230"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..823)
                     /locus_tag="ACUQR0_004125"
     CDS             complement(<1..823)
                     /locus_tag="ACUQR0_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HHV9110918.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1423..1671)
                     /locus_tag="ACUQR0_004126"
     CDS             complement(1423..1671)
                     /locus_tag="ACUQR0_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HHV9110919.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1934..2029)
                     /locus_tag="ACUQR0_004127"
                     /pseudo
     CDS             complement(1934..2029)
                     /locus_tag="ACUQR0_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2026..>2372)
                     /locus_tag="ACUQR0_004128"
     CDS             complement(2026..>2372)
                     /locus_tag="ACUQR0_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HHV9110920.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          610 a          437 c          576 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggtaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttaggtaa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg caatttttcc ttaaggtatg aaaaaaggag
     1081 ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta tataaaaaac
     1141 tgtgttaagg aggctgtcca ttagcggttt tttattcttc tggaaccgcg gcatctttgc
     1201 gtatcttttt gtcttctcac cgtctggtcg gtgttttgct gagactgcta atttcctgtc
     1261 tttgctagtg ctgtactggt actgcccaat catgattggc gggggcgggc ggagttgaga
     1321 ccgcagacac gtcgtatgca agaacgtgct gcggctggct ggtgaacttc cgatagtgcg
     1381 agtattgaat gatttccagc cgttactgat tttacgtgct aattagtgaa caaaccactc
     1441 gtcagcagac tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt
     1501 atcggcggtg cgcgtaacag aaaggccatc gttgctggtg gcttttacga tcacctctac
     1561 atcgtcataa cgtttactga tgcgtcgggt taattcttcc tttaacgcat ccacagcacc
     1621 ggttggcatt ttagtcattt tctctttggc tatgcagatt tcaaaacgca taaaaatccc
     1681 tctgcactgt gtttttatac agtattattt ttaactgtat ggataaacag tgtcaagagt
     1741 gagggaaagt gattttttgg ggcatgcatg gggcaaaaat atttgttgtg gggcattttg
     1801 gggcatgatt gggacacttt gttacatatg aactttgccg attttcatat gacatggcaa
     1861 tccaacgctt tgattattct aaagaaaaca catgctcttg ggcgttcttt agtgattttt
     1921 aaaaatcccg tatcacgcag ttaaagtggc gggcatactc ttcaaggctg gtgatcccca
     1981 accgcaccca tttaggatgt gaccattgcg gtagaccgat gtaaatcata acgcgtgaag
     2041 gatctcttcc acgctacgca cacgggcgat gcgcgggtag atatgattaa tgctgttatt
     2101 gtgttgctcg gcgctggcgg cactacaggc gtcttcggca atcaccagat taaagccaag
     2161 ttcccaggca ttgcgggcgg tggattcaac accgatattg gtcgagatcc cacataacac
     2221 tattgtatcg atacctcggc ggcgcagttg caactccaga tccgtaccgt aaaacgcacc
     2281 ccactgacgt ttgatgattt cgatatcact gtcagttgca cctaatgcag ctggatgttg
     2341 ccaccagttt tcgggcaaca ctttagcggg gg
//
LOCUS       DBKELA010000226         2301 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.231, whole genome shotgun sequence.
ACCESSION   DBKELA010000226 DBKELA010000000
VERSION     DBKELA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.231"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..785
                     /locus_tag="ACUQR0_004129"
     CDS             <1..785
                     /locus_tag="ACUQR0_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9110921.1"
                     /translation="SLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNN
                     HNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALID
                     ANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVP
                     NRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGI
                     SSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            796..1887
                     /locus_tag="ACUQR0_004130"
     CDS             796..1887
                     /locus_tag="ACUQR0_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HHV9110922.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1930..>2301)
                     /locus_tag="ACUQR0_004131"
     CDS             complement(1930..>2301)
                     /locus_tag="ACUQR0_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HHV9110923.1"
                     /translation="RTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHG
                     APVGELLAWVKEDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAAL
                     AAEIHGVKKNALYKYALEQQG"
BASE COUNT          594 a          588 c          552 g          567 t
ORIGIN      
        1 gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa catgaaatgc
       61 gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa caaagtctgg
      121 atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct tatggaacct
      181 tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc gttaccggcg
      241 gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac gcttttgcgc
      301 ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg attgctactg
      361 atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac cgtctctcgg
      421 tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag tttgtggtgc
      481 ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat cgcgtacttg
      541 ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc agcaacgatg
      601 atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct gggatatcga
      661 gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa tgtcagtttg
      721 cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca gcgcagtgcc
      781 attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt gatatccaca
      841 tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg tgcgacaaat
      901 tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac aggacgggag
      961 tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg ctctgcgggt
     1021 aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg acatcagaca
     1081 ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc aaacgacatt
     1141 ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca gataaaaagc
     1201 agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac gtctcgttat
     1261 ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa gccatttctc
     1321 ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg ggtcgatgga
     1381 atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat tcagggaaat
     1441 ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt taattttgga
     1501 ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt tactccagta
     1561 gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc gttgcaaatg
     1621 cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag gcgaagaagt
     1681 tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg agttgcaaat
     1741 attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac cgtcaatatg
     1801 cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt tcagggcaca
     1861 gccgccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta acgcaacgct
     1921 tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt tcttcacgcc
     1981 atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct gtagcagcgc
     2041 cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac cttcgacaat
     2101 cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca gcagctcgcc
     2161 aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca gaaccacgta
     2221 gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac ggtgggtaga
     2281 ttcataaaaa atcagcgtgc g
//
LOCUS       DBKELA010000227         2243 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.232, whole genome shotgun sequence.
ACCESSION   DBKELA010000227 DBKELA010000000
VERSION     DBKELA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.232"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            135..576
                     /gene="tnpA"
                     /locus_tag="ACUQR0_004132"
                     /pseudo
     CDS             135..576
                     /gene="tnpA"
                     /locus_tag="ACUQR0_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(694..1632)
                     /gene="hypT"
                     /locus_tag="ACUQR0_004133"
                     /gene_synonym="qseD"
     CDS             complement(694..1632)
                     /gene="hypT"
                     /locus_tag="ACUQR0_004133"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HHV9110924.1"
                     /translation="MKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKALEA
                     AIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAAAH
                     SLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQSPF
                     CCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGITG
                     RTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIYRM
                     NTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1983..>2243
                     /locus_tag="ACUQR0_004134"
     CDS             1983..>2243
                     /locus_tag="ACUQR0_004134"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HHV9110925.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAH"
BASE COUNT          607 a          489 c          543 g          604 t
ORIGIN      
        1 gtcatattcg ctctgacttt ccagaaccat acggactgcc cgttgacgga cttcggggga
       61 aaaacgagta tttttagtca tcctgtttac ctctttctca ggaagtttag tctccaggat
      121 tcccggggcg gttcatagcg cacacccgat ggaactgtaa atatcacata gttttttgcg
      181 ccaaaatacc gaagacaggt gttctacaga gagaagcgta gagcaacagg cagtattttg
      241 agaaagctgt gtgagtggaa aagcgtacgg attctggaag ctgaatgctg tgcagatcat
      301 atccatatgc ttgtggagat cccgcccaaa atgagcgtat ccggctttat gggatatctg
      361 aaagggaaaa gcagtctgat gctttacgag cagtttggtg atttgaaatt caaatacagg
      421 aacagggagt tctggtgcag gggggactac gtcgatacag tgggtaagaa cacggcgaag
      481 atacaggatt acataaagca ccagcttgaa gaggataaaa tgggagagca gttatcgatt
      541 ccctgtccgg gcagcccgtt tacggaccgt aagtaacgaa gttggatgca aatgtcagat
      601 cgtgtgcgcc tgttagggcg cggctggtaa gagagcctta taggcgcatt tgaaaaacct
      661 ccggctatgc cggaggatat ttattgcacg tcgtcaattg cggcgtgctg gctcttccat
      721 tgatatctgc tggattaaat cgtctggcat atgttcctgt aaaatacgcc agaggttctc
      781 ggcggttcta ttcagtcggg tgttcattcg gtaaatataa gcctgaatgg ggactgtcaa
      841 tttagcagca tcaaggcata tcagacgttt ggtctgtagt tcatcgacga tcgaccagat
      901 cggtaaccag gcaatgccgt atccgttgag cgccatattc tttaataact cgctcatcga
      961 agagataaat atcgtgcgcc cggtaatacc tccaacctct gccagatgac gattgaccaa
     1021 cctgcccata taagatgtgg cggtatagtt gagcaaagga acctggggct gagtgatatc
     1081 gaatacaggg tgcccctgtg cgtcggcggc acataccggg tacaactcag attcaaacag
     1141 tttcaggcag caaaaaggcg attgcataag atcttcatcg cgaaacgaaa taataaagtc
     1201 actcttgcct tcgcgtaacg tatttacggc ctggacaaca tcaatggctt caacatgata
     1261 gacaaattct ccgccatagg cggtaagtga atgcaccagt ttgggtaata cgctcaggga
     1321 tagtgagtgc gcggctgcaa ttttaatgtt gggaacgccg agtaaactct ggccgtttaa
     1381 ctcccccaaa ttgcattcaa gctgctgtaa taagctgcga gtctgggaat gaaagagctt
     1441 gccctcttcg gttaattgta aaggggtggt cgtgcgatcg aataaaacaa cgccgatcgc
     1501 tgcctccagc gccttaattc gtcggctgaa tgcgggttgc gaaagattac gctcttttgc
     1561 cgcctgagaa aaatggcgac aggcttccag tgtcaggaaa tcgtataacc attttgtctc
     1621 gatattcttc attaccatgg cacctgtatt ttcaatccaa agacataata aaacatcccg
     1681 gcaataaaaa taatgttctc aattagcaca acctggcctt tatcattcgt tttgtgcata
     1741 accccttcat ttccggtatt gacgttgata tcacatttcg ccaaaaaaac gaaatttttc
     1801 cgtcagataa ccagaataca tcatcactat ggtgaataag cggaatgttc catagctgca
     1861 tcatgcgtgt cttgcatcgc cttgtacaac cttgcatttc tcatcgccca tttcgactgt
     1921 taaaaaagag agcagcagtt atttaccgcg tattgtcatt aacgttttga gaggcaggta
     1981 ttgtgaaagg tttaattggt gtactcggtg gaatggggcc ggcagctacc gtcgacctgt
     2041 ttaataaatt tgtaactttt accgctgcac aatgtgatca ggaacatatt cctttaatta
     2101 tttcttcgat cccggatatt cctgaccgta ccgatgcatt aatgcatcat gggcgttcac
     2161 cattacccgt tatgcgtgat tatatgcata agctggaaga tgccggggca gaatgtattg
     2221 ttattccctg taataccgcg cat
//
LOCUS       DBKELA010000228         2126 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.233, whole genome shotgun sequence.
ACCESSION   DBKELA010000228 DBKELA010000000
VERSION     DBKELA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.233"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            275..469
                     /locus_tag="ACUQR0_004135"
     CDS             275..469
                     /locus_tag="ACUQR0_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110926.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            782..1146
                     /locus_tag="ACUQR0_004136"
                     /pseudo
     CDS             782..1146
                     /locus_tag="ACUQR0_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611151.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(1268..1604)
                     /locus_tag="ACUQR0_004137"
                     /pseudo
     CDS             complement(1268..1604)
                     /locus_tag="ACUQR0_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          437 g          570 t
ORIGIN      
        1 gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt tatcaccaga
       61 agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca ttatcgaaca
      121 gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat ctcctttagt
      181 tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt tttcattagc
      241 acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct
      301 ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac gtgccgcgct
      361 gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata atggcaaaaa
      421 aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac aacaatctgc
      481 atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt ctttatattc
      541 actgacgagc ttgccggggc acctttgttt gccggtcatt ctggtatcat gcagatattt
      601 acggcgtaca agcccactag catgacattg taataaggcc tgagcctaac gctttaatgt
      661 gtgtcataca ctcagtggcg taatcgttga caatatggtg catgtaatcg taagcgtcca
      721 gcgaaatcca tctggtcata atctgaagca tccgacaaag tggtgtccac caaataagtg
      781 atctgcgccc aatatcctga gccggaactt tgacaaaggc caacgaaaaa tgggagcgga
      841 tgttacagag ttctcggtac aggggaaaag tggtacctgt cgccagttct ccgatctgtt
      901 taaccgggaa attatctcct acagcttgtc agaaaggtcg gtgatggagc atcgttaata
      961 cgatgctata cgatgcattc acagtgcccg gccagaggat gccccgtcgc tgcatatgga
     1021 tcagggttgg caatatcgaa tcgcaggcta taggcaaagt taaagcccca tggagtagca
     1081 caaaatatgc cgcacaaagg aaacggtctg aataacgcag tgatgaagaa cttcttcagc
     1141 acactgctaa aacgcatagt gatcgagcgc tgattctggc gaacaactga actaatacat
     1201 cagaatatgc attatgttaa ataaatatga aaagatggtt taaatacccc gttacttttg
     1261 acttacacta tacggtatcg catcgtttaa tattcgcacc ggccagattt ttatttctat
     1321 tagttgtcac aatactgaat gcgtacgacc acagtattct ggctcctgtg tggttatgct
     1381 ttaattctgc gttccgggca gataagcagt tgcttgcagg aatccttctt gtgttaatgt
     1441 cagttcccct ttttaccagt gctgatttcc acattccgtc caacagagct tatagccttt
     1501 ccctggatta tagcattgtc cggctgaagt tctttttgaa taataataga agcactgctg
     1561 gcagatccag tccgtttttc ataacccact gtactgataa ccataatcta atcagtagaa
     1621 attgagtcga aaataagcac tactccatac aggataatta gaggtcagtt tgattattca
     1681 caattcatca tcagcatttt ctatttctga cgaaatcaac atgaaaataa ccatatatga
     1741 taattattat aataacggct ttaattggaa tacatatatt acaacgtatt atatataatt
     1801 ggtattctgg gaactatatt ctcaaaatat agtagaaacg aggtatgttt ctggtggaaa
     1861 ggacagtggg attaaaaagt aagaattgat aaaaaaaacg ccagcaacac atagtgccgg
     1921 cggagggaat accccatgga gaaaatgtga tgcctagagc atcatgaata tataattaaa
     1981 aatagttagc gttgtcaata ctttcacaaa aataattttc gtagtataga aagatatttt
     2041 tatgcatgac ctacctgaat ttgctccggg tagaggttat aaataaaaat tgaatcacga
     2101 caaacacaat attcatagta tggcga
//
LOCUS       DBKELA010000229         2041 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.234, whole genome shotgun sequence.
ACCESSION   DBKELA010000229 DBKELA010000000
VERSION     DBKELA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.234"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(1..405)
                     /locus_tag="ACUQR0_004138"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="ACUQR0_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            591..1433
                     /locus_tag="ACUQR0_004139"
     CDS             591..1433
                     /locus_tag="ACUQR0_004139"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HHV9110927.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1436..>2041
                     /gene="capU"
                     /locus_tag="ACUQR0_004140"
     CDS             1436..>2041
                     /gene="capU"
                     /locus_tag="ACUQR0_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HHV9110928.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
BASE COUNT          575 a          384 c          454 g          628 t
ORIGIN      
        1 ttgtagctgc tgcgatggac cccgaacaca tggcagagag tggccacagg ataacgcgcc
       61 ctgagtttcc cgattatcga aaactgttca gggagtctga catcaagagc gcggtagcct
      121 ttttaatatt tcattttcca tttcaatacg ttgtagcttt ttcctgagct cacggatttc
      181 aatttgttcc ggggtaatgg gggaggcttt tggtgttttt ccctgccgct catcacgtaa
      241 ttgtttcacc catcgcgtca ttgtggaaag gccgacatcc atagcgctgg ctgcatctgc
      301 cacggtgtat ttctggtcaa cgaccagttg agcggattcg cgtttaaact ctgcgctgaa
      361 atttcttttt tcattatggc acctgtgttg ttctgaggtg agcatatcac ctctgttcag
      421 gtagctaaat ttagtgtgcc acctcaaggt tcttagtgag ccgtttttaa aagatatgat
      481 aatatattat tgtttataaa ttgttgatgt ttgatttagt agtatataaa gttcaccacc
      541 tttattaggc tttcctttta tctctactta ctgggtgcca gaaactttaa attttaaacg
      601 gagggggcat tttgtttaaa gcaaatcatg ttccggtatt aatgtatcat catgtcagcc
      661 attgcccagg actggttaca ctttctcccg agacattccg gaaacaaatg aaatggttgg
      721 ctgaaaacaa ctggaaaaca ctgtcatcag atgaactgga gtttttttat cggggcggaa
      781 aacttccccg taaaagcgtt atgttgacat ttgatgatgg ataccttgac aactggtttc
      841 aggtatatcc tttattaaac gagtttaatc tcaaggcaca tattttttta attaccagtt
      901 ttattggaaa tggtccggtt cgtcactctc ctggaaagga atattctcat cgcgattgcg
      961 aacatcaaat agcaaccggg aatgctgata atgttatgct tcgctggtct gaagtgaacg
     1021 aaatgttgca atctggtctg gtcgaatttc atgttcatac acatactcat acacgttggg
     1081 ataaaaagtt cacttcacga gaagaacagt gtaaacatct tcggcaggac ttattgagcg
     1141 ggagagaata tttaaaaaaa atgacaggaa aatgcagtaa acatttgtgt tggccggaag
     1201 gttattataa caaggattat attcagatcg ctgaagaact tggattccat tatttatata
     1261 caacagaaag aagaatgaat gctcctgcta aaggaactac acggatagga agaataagca
     1321 ccaaggaacg ggaaaattgc gtatggctga aaagacgact tttttattat acaacaccgt
     1381 ttttttcatc gttgctggcc cttcataagg ggccaagatt accggatgac taataatgaa
     1441 tatactattt acggaatcat caccgaatat aggtggtcag gaattacagg ctgttgctca
     1501 aatgaaggcc ctgaagaaaa tggggcattc agttctgctt gtctgcaggg agaacagcaa
     1561 aattgctttt gaagccagta agtttggaat tgatatcaca ttcgcgttat ttcgaaacag
     1621 tcttcacatt cctactgtat ggagattact cggaatagtt catagttttc agcccgatgc
     1681 aatcgtttgt cacagtggac atgatagtaa tattgttggt ttggtacggt tttttacctg
     1741 gaaacatcca ttcagaatta tcaggcaaaa gacatatttg acacgaaaaa caaaattttt
     1801 ttcaataaat catttttgcg atgaggtgat tgttcccgga acaaatatga agacacatct
     1861 ggagcaggaa ggatgtcgaa ctcgggttac tgttgtgcct cctggctttg atttccagga
     1921 attatacgtt gattcccgaa acagtttgcc tccgagtgtt ctttcctggc tggcgtcccg
     1981 aagaggatgc cccgtaattg ctcaggtagg aatgttgcgc ccggaaaaag ggcacgaatt
     2041 t
//
LOCUS       DBKELA010000230         2033 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.235, whole genome shotgun sequence.
ACCESSION   DBKELA010000230 DBKELA010000000
VERSION     DBKELA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.235"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>2033
                     /gene="sat"
                     /locus_tag="ACUQR0_004141"
     CDS             <1..>2033
                     /gene="sat"
                     /locus_tag="ACUQR0_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HHV9110929.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          746 a          358 c          401 g          528 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca gat
//
LOCUS       DBKELA010000231         1966 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.236, whole genome shotgun sequence.
ACCESSION   DBKELA010000231 DBKELA010000000
VERSION     DBKELA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.236"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..120)
                     /locus_tag="ACUQR0_004142"
     CDS             complement(<1..120)
                     /locus_tag="ACUQR0_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110930.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVS"
     gene            complement(93..1118)
                     /locus_tag="ACUQR0_004143"
                     /pseudo
     CDS             complement(93..1118)
                     /locus_tag="ACUQR0_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1176..1966
                     /locus_tag="ACUQR0_004144"
                     /pseudo
     CDS             1176..1966
                     /locus_tag="ACUQR0_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          489 a          474 c          499 g          504 t
ORIGIN      
        1 gctaacctga tggatcgcca tgcggatttc attaatcgct ttgatgcgat catcttccgg
       61 caagctggcg agaaattgcg ttaaatcctg cgttaatcgt tgttgcatag tatcccccat
      121 tcctgacgtt ttccttttat ccgttcgtta taacgctgat agttcttcgc tttcgtagga
      181 ctaaatgata atgcccgaca ccagtaatcg ttattgagta aaactttgca tatccgccgc
      241 caggagggga tatctttcgc gccaatgtcc ccttgctggg tttgtggaac ctcgatgcct
      301 tttttctgat accagtgcaa ataaatggcg attttattgc ggtaatgctc agctgttttt
      361 tccggcatgc tattgagcag caataatgca tattcttgcc agtctaaatg ttctggtttg
      421 aggattttcc gatgcccata gaaatgattg tcatgtccgg cgtaaatgcc gccacttttt
      481 accccgctga ctcgtgcgca catagcagcc cagcgatccg gttcgataac gtgacagagc
      541 cataatcctt gtcgttgctc cgggccaaaa ggttcgcaaa ttcgcatatg acgcagagga
      601 acgcctgcct gatacattag gttatacagt gggttgcaga gattttgatg atttgcatat
      661 caggtccaga tatcagccac tttccagtcg taaatggggt aaatgtacca gctatgaccg
      721 cctggtgcgg ccgtagtcca tggtttatcg tcggcaaaac gttgtttatt taaactggcg
      781 atggcgacaa aacggttgta ggactcatcc gcacggatgc cgatcatcat cgccgccgga
      841 cgtttttgtg aaaaccattc ggcaaactca cgtacaaatt gttcgaaggt catgcctggc
      901 tggtaaaagc agaaaaagtc aggagcggtt atcgcatctt gcgggggctg acgcacccat
      961 tcgacatcag gttcccagca ctgccattcg ggttggtatt gtgaaaggga attttgcgtc
     1021 gtaagcggga gcgcaaccca gtaaaactct tcgatgacat cggtgtacaa ctcgcgcagg
     1081 gactgaacat agttaatggt gcaagagaat tgtgaatcgc cacgggttta acagacacct
     1141 cagagtcatt taagatggct taaagagagg tgcccatgag cggtaagcgt tatcccgaag
     1201 agtttaaaac tgaagcagtc aaacaggttg ttgatcgcgg ttattctgtt gccagcgttg
     1261 caacacgtct cgatatcacc acccacagcc tttatgcctg gataaagaag tacggtccgg
     1321 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
     1381 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
     1441 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
     1501 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
     1561 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
     1621 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
     1681 gtgcggagta aacagagtct ggagactgat gaaacgtgtc ggaataaagg ctcaggtcgg
     1741 ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc gtgtcaccca acaggctcca
     1801 gcgacagttc aatccggatg ctcctgatga gcgttgggta acggacataa cctacatcag
     1861 gacccacgaa ggctggctgt atcttgccgt tgttgttgat ctgttctcac gcaaaattat
     1921 cggctggtcc atgcaatccc ggatgacaaa ggacattgtc ctgaac
//
LOCUS       DBKELA010000232         1941 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.237, whole genome shotgun sequence.
ACCESSION   DBKELA010000232 DBKELA010000000
VERSION     DBKELA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.237"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..576
                     /locus_tag="ACUQR0_004145"
     CDS             <1..576
                     /locus_tag="ACUQR0_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip protein"
                     /protein_id="HHV9110931.1"
                     /translation="GGNPPAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRV
                     LGKLSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFR
                     GAKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVS
                     AWLVNGWYPTASISDLLVVVMKKSTAGISIS"
     gene            644..1243
                     /locus_tag="ACUQR0_004146"
     CDS             644..1243
                     /locus_tag="ACUQR0_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HHV9110932.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1307..>1941
                     /locus_tag="ACUQR0_004147"
     CDS             1307..>1941
                     /locus_tag="ACUQR0_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HHV9110933.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT          468 a          457 c          590 g          426 t
ORIGIN      
        1 ggcggtaatc ctccggcatt ttccctgaca cctggcggac ggctgacggc gaaaaatgcc
       61 gatatcagcg gtaacgtgaa cgcgaactcc gggacgctca acaacgtcac gattaataag
      121 aactgtcggg ttctgggaaa attgtccgcg aaccagattg aaggcgatct cgttaaaaca
      181 gtgggcaaac ctttcccacg ggactcccgg gcaccggaga ggtggccatc agggaccatt
      241 accgtcaggg tttatgacga tcagccgttt gatcggcaaa ttgttattcc cgcggtggcg
      301 tttcgcggtg ctaaacatga gcggaagaat aacaatattt attcgtcatg ccgcctgata
      361 gtgaagaaaa acggtgctga aatttataac cgaacgaccc tggataatac gctgatatat
      421 acgggtgtta ttgatatgcc tgccggtcac ggtcacatga cgctggagtt ttctgtatcg
      481 gcatggctgg taaatggctg gtatcccaca gcaagtatca gcgatttgct ggttgttgtg
      541 atgaagaaat ccacagcagg tatcagtatc agctgaattt tataacccag atacgggcac
      601 cagaaatggt gcctttttta ttgcagaaaa gcgagaggta attatgcgta aactttatgc
      661 tgccattttg tccgcagcca tctgtctggc tgtatccggt gcgcctgcat gggcgtctga
      721 acatcagtcc acgctgagcg cggggtatct tcatgcctcg acgaacgctc ccggcagtga
      781 tgatctgaac gggattaacg tgaaataccg ttatgagttt acggacacgc tggggctggt
      841 gacgtcattc agctatgcca acgctgaaga tgagcaaaaa acgcattaca gtgatacccg
      901 ctggcatgag gattccgtgc gtaaccgctg gttcagcgtg atggcggggc tgtctgtgcg
      961 cgtgaatgaa tggttcagcg cgtatgcgat ggcgggcgtg gcttacagcc gtgtgtcgac
     1021 cttctccggg gattatctcc gcgtaactga caacaagggg aaaacgcacg atgtgctgac
     1081 cgggagtgat gacaaccgcc acagcaacac gtctctggcg tggggggctg gcgtgcagtt
     1141 taacccgacc gaatccgtgg ccattgacct tgcttatgaa ggttccggca gtggcgactg
     1201 gcgcactgac ggtttcatcg tgggtgtcgg ttataagttc tgattagcca ggtaacacag
     1261 tgttatgaca gcccgctggt tcaggcgggc tttttgtggg gtgaatatgg cagtaaagat
     1321 ttcaggtgta ctgaaagatg gggcgggtaa acctgtcgta aattgtgcga ttgaactgcg
     1381 ggccagaaga accagtccga ccgttgtggc acacgttgtt gccacttgcg tgacggacaa
     1441 taacggtgct tatgtgattg aggctgagcc ggggtattac gaggttgcgc ttcactgtaa
     1501 cggctggcag ccaacccgtg tcggggatat tgatgtggca ccgactgatg caccggggac
     1561 actgaacgcg tttctgaatg caccaaagga tggtgattta cgtccggagg tgatgaagcg
     1621 ctttgaggaa atggtggcgc aggcgcagca gagtgccggg gctgcagccg gaaacgcaca
     1681 gcagacggcg caggatgtgg cggcagccgc aacggcccgt gatgatgcac aacgttttgc
     1741 ggagaaagcc cgacaggatg caaccgtcac agctgaggac agaaaggcca ctgcggaaga
     1801 tgtgacaagc acaggagcaa atgcagccgc agccggacag agcgcacagg atgccgcagg
     1861 ttatgcccgc gcagcagaac aggccaaaaa tgacattgat gctgcgctga ccggcactct
     1921 gaaaatggct aaccatctgt c
//
LOCUS       DBKELA010000233         1913 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.238, whole genome shotgun sequence.
ACCESSION   DBKELA010000233 DBKELA010000000
VERSION     DBKELA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.238"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(53..1702)
                     /locus_tag="ACUQR0_004148"
     CDS             complement(53..1702)
                     /locus_tag="ACUQR0_004148"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HHV9110934.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DBKELA010000234         1862 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.239, whole genome shotgun sequence.
ACCESSION   DBKELA010000234 DBKELA010000000
VERSION     DBKELA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.239"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(188..1486)
                     /locus_tag="ACUQR0_004149"
     CDS             complement(188..1486)
                     /locus_tag="ACUQR0_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HHV9110935.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
     gene            complement(1552..>1862)
                     /locus_tag="ACUQR0_004150"
     CDS             complement(1552..>1862)
                     /locus_tag="ACUQR0_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HHV9110936.1"
                     /translation="IESLGFTCVSPDADGQKKVMESIYSLKAGNIAHAQKLMNEQAEI
                     LFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTASLVRAGIKWYENRVGKHYLLTQ
                     "
BASE COUNT          561 a          476 c          413 g          412 t
ORIGIN      
        1 gatagatgca gctaatacac aacataacca agaaataaat cattaaacga caacacttaa
       61 taccatattg tgaagatgtt cagacatggc ggaattcccc tattctttgt tggcgcttac
      121 aacagactat attccgccat atctgtcttt attgtgtata aaccatcgat actgatgttt
      181 gatagtgcta aataatcatt ggcgcaatca caaagcctaa tgccactcca gcaataattc
      241 cccccaaccc aggcagcata aatggatgat tcagcacata tttgcctaca cgagttgtgc
      301 cggtagtatc aaattccatg gcagccagtg atgttggata cgttggcagt acaaatactc
      361 cggtgacggc cacataggac gccagaattg cccaggtggg cacgtcaagc gcgacggcaa
      421 gcgggataat taacggcgtg gtcgcacctt gcgagtagag cagcgcgcaa gtgccaaaaa
      481 gaaccagcgc caacaacatc ggatacgcct gcaaaatatc accagcaata tctttaatct
      541 cagttaaatg cgcatcaata aaagtggtgc ctaaggttac aatgccgaga attaccgcca
      601 gcgaactcat ccccgcacgg aatgtggagg tgagaataat ggcatcggtc gccgtcttac
      661 acagcaccac catcaggcag gcggcagaga gcatacagat aataatgata tcgcgtgttt
      721 ccatcggttt accgatatca aaacccggac gcagttgggg gaaggcagca agaataacaa
      781 tagcgaccgt cgccagcagg aacaatccga cggagagcgt cgcacctggc ttgatgctgc
      841 tattattctc gtatttttgc accagtcctt tttgcaggcg ctcaaggtag acctcgtcat
      901 cctgcaactc acatccctgt cgggaagcaa taaaagcggc gaccatcgcg gcagcaaaag
      961 aggtgggaag acataccgac atcacttgaa taaaactgac accgtttacc tccataatag
     1021 tgagcatagc cgccattgcg gcgctgatcg gcgaaccgga aatcgcaacc tgcgaagcga
     1081 caaccgaacc ggcaagcgtg cgggaaggac ggataccgga ctctttcgcc acttcagcaa
     1141 tcaccggcag cgtggaaaaa acaataaacc cagtaccggc cataatggtc atagaccagg
     1201 tgataatcgg cgcgattatg ttgatatatt tcgggttacg ccgcataaaa ttaccagcga
     1261 cgcgtaccag ataatccatt ccgcctgcgg cctgtaaggc tgaggccgcc acaacaacgg
     1321 tcataataat caggatcacg tcgactggcg gcgaccccat aggcagacca aatcccagag
     1381 tcagaatggc aagcccaagc ccgccacata aaccaatacc gattcccccc tggcgaatac
     1441 caaaaaatat tgcaccaagt actacgataa tttccagcca aaccataatg ttctccgtta
     1501 taaatataat tcagttaaaa gcgaataact ttctggctgc gatttaaatg attattgggt
     1561 taaaagataa tgtttaccga cacgattttc gtaccatttt attccggcac gaacgagtga
     1621 cgccgttgag tcaatatagc gggaaggctg ctctttaacg gcttgcgcca gaataaccgg
     1681 aatttctgta caaccaagaa caaggatttc tgcaccgcgg gaaaataata tttcagcctg
     1741 ctcattcatc aacttttgtg cgtgcgctat attgccagct ttcaaggaat aaatactttc
     1801 catcactttt ttctgaccgt cggcatcggg gctgacacag gtaaatccca gcgactcaat
     1861 tc
//
LOCUS       DBKELA010000235         1858 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.240, whole genome shotgun sequence.
ACCESSION   DBKELA010000235 DBKELA010000000
VERSION     DBKELA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.240"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..698
                     /locus_tag="ACUQR0_004151"
     CDS             <1..698
                     /locus_tag="ACUQR0_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HHV9110937.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGN
                     TTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFN
                     GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPV
                     LDVSDISVKLQEA"
     gene            700..1305
                     /locus_tag="ACUQR0_004152"
     CDS             700..1305
                     /locus_tag="ACUQR0_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HHV9110938.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1292..>1858
                     /locus_tag="ACUQR0_004153"
     CDS             1292..>1858
                     /locus_tag="ACUQR0_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHV9110939.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRG"
BASE COUNT          460 a          443 c          513 g          442 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagttg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
     1321 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
     1381 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
     1441 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1501 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1561 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1621 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1681 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1741 ccttcagaat aaagggacgg cacgggataa tcttcaggtt tacagtaaag agcagtcaga
     1801 tcaacgctat gttcatcggg aaggcgataa aataaccgga gagctgaaaa tccgtggt
//
LOCUS       DBKELA010000236         1805 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.241, whole genome shotgun sequence.
ACCESSION   DBKELA010000236 DBKELA010000000
VERSION     DBKELA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.241"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            173..1198
                     /locus_tag="ACUQR0_004154"
     CDS             173..1198
                     /locus_tag="ACUQR0_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHV9110940.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1805
                     /locus_tag="ACUQR0_004155"
                     /pseudo
     CDS             1266..1805
                     /locus_tag="ACUQR0_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          461 g          503 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgc
//
LOCUS       DBKELA010000237         1794 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.242, whole genome shotgun sequence.
ACCESSION   DBKELA010000237 DBKELA010000000
VERSION     DBKELA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.242"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(110..577)
                     /locus_tag="ACUQR0_004156"
     CDS             complement(110..577)
                     /locus_tag="ACUQR0_004156"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HHV9110941.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACUQR0_004157"
     CDS             complement(643..882)
                     /locus_tag="ACUQR0_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HHV9110942.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACUQR0_004158"
     CDS             complement(1017..1622)
                     /locus_tag="ACUQR0_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HHV9110943.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(1737..1793)
                     /locus_tag="ACUQR0_004159"
     CDS             complement(1737..1793)
                     /locus_tag="ACUQR0_004159"
                     /inference="COORDINATES: protein motif:HMM:NF015365.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HHV9110944.1"
                     /translation="MELHDKVIGHYLNIKHYQ"
BASE COUNT          569 a          397 c          317 g          511 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata ttttttggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaatt aggtaatgac tccaacttac
     1741 tgatagtgtt ttatgttcag ataatgcccg atgaccttgt catgcagctc cacc
//
LOCUS       DBKELA010000238         1784 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.243, whole genome shotgun sequence.
ACCESSION   DBKELA010000238 DBKELA010000000
VERSION     DBKELA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.243"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            51..1784
                     /gene="lpfC-O113"
                     /locus_tag="ACUQR0_004160"
                     /pseudo
     CDS             51..1784
                     /gene="lpfC-O113"
                     /locus_tag="ACUQR0_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
BASE COUNT          468 a          401 c          446 g          469 t
ORIGIN      
        1 gtgacccgat aaaaatgacg ctgtaagcaa gggataagga taggcgtatt atgatgacga
       61 ccagaatagt ggttggcctc acggcaggga cgtgtctgat tttctcgcaa aacctgatgg
      121 ccgaggtcag tgtattcaat ccggcgcttc tggaaatcaa ccatcaatcc ggagtcgata
      181 ttcgccagtt taatcgggca aacctgatgc ccccaggtgt ttatagcgtt gatattttta
      241 tcaacggtaa aatgtttgaa cgtcaggatg tgacatttgt tcaggataat ccagatgctg
      301 atctgcacgc ttgctttatt gccattaaaa aaacactgtc ctcctttggc ataaaagttg
      361 atgcgctcaa atcgttcaat gatgtggatg agacggtttg cctcgatcct gctccacgta
      421 ttgaaggctc atcctggcag tttgacagtg ataaattgca gctgaatata tccattcacc
      481 aaatctacat ggacgcgatg gcttatgatt acatcagccc cacgcgttgg gatgagggga
      541 ttaatgcgct caccatcaac tacgattttt ctggttcaca tacactacgt tcagattatg
      601 gttcacaaga gacagatacc agttatctca atctgcgcaa tggactgaat attggaccgt
      661 ggcggctacg taattacagt actttaaaca ccagcgatgg ccgtgcggaa tacaactcca
      721 ttagtacctg gatacagcgc gatattgccg cgttaagaag ccagattatg attggtgata
      781 cgtggacggc gagcgatatt ttcgacagta cgcaaattcg cggcgcgcgt ttgtatactg
      841 ataacgatat gctacccgcc agccagaatg gctttgctcc tgtggttcgt gggattgcaa
      901 agtccaacgc caccgtcatc attcggcaga atggctacgt gatttatcag tcagccgttc
      961 cacaaggtgc ttttgagatc accgatctca acaccgcaag tacaggtggc gatttggacg
     1021 taaccatcaa agaagaagac ggtagcgaac aacgattcac ccaaccttat gcttcattgg
     1081 cgattcttaa acgtgaaggt ctgacagatg ttgatgtcag cgtgggtgaa ttgcgcgatg
     1141 aagacggatt tacaccggac gtccttcagg cgcaaatact tcatggtttt tcccacggga
     1201 tcactttata tggaggtatg caggctgctg aaaattatgg ttctgcagct ctgggtgtcg
     1261 gtaaagatct tggcgctttg ggcgcaattt ctttcgatgt gacacatgct cgtgcgaatt
     1321 ttagccatga tgatacagaa acgggtcagt catatcgctt tctctattca aaactatttg
     1381 acgacacaga cactagcttg cgcctggttg gctatcgtta ctccaccgag ggctactata
     1441 ccactcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atag
//
LOCUS       DBKELA010000239         1684 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.244, whole genome shotgun sequence.
ACCESSION   DBKELA010000239 DBKELA010000000
VERSION     DBKELA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.244"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACUQR0_004161"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACUQR0_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HHV9110945.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1684
                     /locus_tag="ACUQR0_004162"
     CDS             1494..>1684
                     /locus_tag="ACUQR0_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHV9110946.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLR"
BASE COUNT          484 a          286 c          397 g          517 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttg
//
LOCUS       DBKELA010000240         1642 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.245, whole genome shotgun sequence.
ACCESSION   DBKELA010000240 DBKELA010000000
VERSION     DBKELA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.245"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            99..287
                     /locus_tag="ACUQR0_004163"
     CDS             99..287
                     /locus_tag="ACUQR0_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110947.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            297..1495
                     /locus_tag="ACUQR0_004164"
     CDS             join(297..1113,1113..1495)
                     /locus_tag="ACUQR0_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HHV9110948.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDIS
                     LLRYMPA"
BASE COUNT          385 a          418 c          508 g          331 t
ORIGIN      
        1 tgcaaattga aaaccgcccc tgttcgtcct gttgttgccg cacgctccgc agaatatgta
       61 tgctctgagg acctgtttta ttaaggactc atcctcttat gttgacccgc aaaagtatag
      121 ataccgttct gctctctgtt ggtgctgaga agctctcaca acgtgaatgg gactggatga
      181 agatgctcaa acccatggac ccaccacctg cgatggtcgc ggcctccatc ctggagcgcc
      241 gtggcgacac agcagccctt acccggctac aggataccgg gggttaatcc gtcctcatgt
      301 tgtccgcttt tactccccgc ccgctgaaac gtctgttcac ggccaaccag tgctggacat
      361 ccttcctgga tgcgggcggt ctgcgcgata tcgaggttga ggctgtcaca aaaatgctgg
      421 cctgcggcac gcggatactg ggcgtgaagg aatacatctg cgataaacct gagtgccccc
      481 acgtcagata cgtcactaac tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg
      541 ccacagacct gtggatagcg acacagctga atcgtcttcc tgactgcgac tgggtacacc
      601 tggtcttcac cctgccggac acgcagtggc cggtgttcga aagcaaccgg tggctgctga
      661 atgacgtgtg ccgtctggcg gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg
      721 aacccggtat cttctgcgcc atccacacgt atggccgtcg tctcaactgg catccgcatg
      781 tacatgtgtc tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa aagctgagct
      841 tcctgaaaga cgcgatgcgt tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag
      901 cgtggtcaga ggggctggcg atgccggagt cgttgtcaca tatcacgacg gaatcacaga
      961 ggagaagcct ggtgctgaaa gccggaggaa aatactggca tgtgtacatg tcgaaaaaaa
     1021 cggccggcgg gaggaatacg gcgcgctacc tgggtcgtta tctgaagaag ccgccgatag
     1081 cggcctcccg gctggcacat tacaacggag ggggagcctg agcttccgtt acctggacca
     1141 caaaacggga gaaacggcga cggaaacgct gacacagcgt gagctggtcg cgaggctgaa
     1201 acagcacatc ccggagaagt tttttaagat ggtgaggtac ttcgggttcc ttgccaaccg
     1261 ggtgtgtgga gagaagctgc cgcaggtgta ccgtgctctg gggatggata aaccggaacc
     1321 agtggcgaaa gtgtgctatg cacaaatggt gaaacagttc ctgagtcgtg acccgttcga
     1381 atgcgtgctg tgtggcggcc ggatggtata ccgccgggcc atcgcgggac tgaatgtgga
     1441 ggggctgaag aaaaacgcgc gggatatcag tctgctgagg tatatgccgg cctgacacag
     1501 gctaagtgtg cccgggaatc ggtgcaagta taaaaaacag cctgttgcgg tataagaatc
     1561 gaccatgcag agagagaaaa caccggagtg gagggaaaag cagaaaagct cacgtggcat
     1621 aaggcgtgga cagcggtatc gt
//
LOCUS       DBKELA010000241         1612 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.246, whole genome shotgun sequence.
ACCESSION   DBKELA010000241 DBKELA010000000
VERSION     DBKELA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.246"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..130)
                     /locus_tag="ACUQR0_004165"
     CDS             complement(<1..130)
                     /locus_tag="ACUQR0_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110949.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESG"
     gene            complement(132..287)
                     /locus_tag="ACUQR0_004166"
     CDS             complement(132..287)
                     /locus_tag="ACUQR0_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HHV9110950.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(490..897)
                     /locus_tag="ACUQR0_004167"
     CDS             complement(490..897)
                     /locus_tag="ACUQR0_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9110951.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            974..1201
                     /locus_tag="ACUQR0_004168"
     CDS             974..1201
                     /locus_tag="ACUQR0_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HHV9110952.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1185..1607
                     /locus_tag="ACUQR0_004169"
     CDS             1185..1607
                     /locus_tag="ACUQR0_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HHV9110953.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
BASE COUNT          389 a          342 c          423 g          458 t
ORIGIN      
        1 cgccagattc cagcgcttct ttagccagcc agatgcgtgc cccagtgcct gcatttggct
       61 ccagttgctg gaggcgtttt gcatcttcta ggagtagagc gataacgtgt tttaattctg
      121 tctcgttcat tttactcacc tgaatgtctt cccaaccaac gacgtgcgcc agcttcggtt
      181 ttaaacgttt tgcttttggt atacgttatg gcggtgaatg tgccgtcctg gttggggaac
      241 acgccgtaca ccggagattc gttgttgcca agatcgatag tattcatgtt gaccccattt
      301 ccccttaacg ccggggtagc ggaactgttt gctgagaaca ccgtgcggtg tcttgatgaa
      361 taaaatttag aataacctaa ggagggtgat caagattttt atgtagaaaa acctaagttt
      421 tttgatgtaa aaaacacaag tgtttgaaag tttgtgcttt ttattacagg gtgtggagaa
      481 aaaaggggat tatttgtttg cgcttctttt gcgagctttg agtagttctt caaaaagttt
      541 gttgaaattt tcaactcgag cacgcatctc tgacaacagg gccttttgct ctgactcagg
      601 cagtgcgtcg aacagttgaa gtaactcttt ttgatcttct gtcagaatgg ctggctgatt
      661 atccgggatc ggttcgcctg gttgtttatc ttcatcccca aaaagaagcc aagtcggtga
      721 gcactgaagc gcttggctca gtgcgaataa tcttttcccc gccggctgtg tttcatctct
      781 ttcccattga gaaattgtta cgtgagccac tttgaccagc ttacctaatg cggcctgaga
      841 cagttttaat tttttacgcc tatgtaagag gcgagcaccg aaggtttcgt ttttcatatt
      901 agggaattct aatttttctt gacttaggtt tctctacggc ctggtttcct taggaaaatc
      961 taaggagctc gatatgttga aaattgatgc tatagcgttt tttggcagca aaacaaagct
     1021 tgccaatgcc gcaggagtta ggttggcaag cattgctgca tggggggaac tggttcctga
     1081 aggtcgcgcg atgcgcctgc aagaggcatc tggcggggaa cttcagtacg accccaaagt
     1141 ttatgacgaa tatcgtaagg caaaacgacc tgggaaggtg attcatgaaa atcaggcatg
     1201 agcacatcga atcagtgctg ttagccttgg cagccgaaaa agggcaggcg tgggtcgcta
     1261 acgcaattac tgaagaatat ctgcggcagg ggggcggcga attgtctctg gtaccaggca
     1321 aggactggaa caatcagcag aatatctatc accgttggtt gaaaggtgaa acgaaagcgc
     1381 aaagggaaaa aattcagaaa ctgatccctg cggttctggc aattcttccg cgtgagctgc
     1441 gtcaccgact ctgtatcttc gataccctgg aacgccgtgc attactggcg gcgcaggaag
     1501 cgttgagtac ggcaattgat gcgcatgatg atgctgtcca ggccgtttac cggaaagcac
     1561 atttcagcgg tggtgggtcg cccggcgatt ctgtcgtagt gcattgattg aa
//
LOCUS       DBKELA010000242         1602 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.247, whole genome shotgun sequence.
ACCESSION   DBKELA010000242 DBKELA010000000
VERSION     DBKELA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.247"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(334..471)
                     /locus_tag="ACUQR0_004170"
                     /pseudo
     CDS             complement(334..471)
                     /locus_tag="ACUQR0_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="ACUQR0_004171"
     CDS             534..716
                     /locus_tag="ACUQR0_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HHV9110954.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="ACUQR0_004172"
     CDS             complement(817..1146)
                     /locus_tag="ACUQR0_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HHV9110955.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1602)
                     /locus_tag="ACUQR0_004173"
     CDS             complement(1318..>1602)
                     /locus_tag="ACUQR0_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110956.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          376 g          422 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcagaaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     1561 ctgggtatca caaagttttt gcgcgttcaa taacacgaaa tg
//
LOCUS       DBKELA010000243         1598 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.248, whole genome shotgun sequence.
ACCESSION   DBKELA010000243 DBKELA010000000
VERSION     DBKELA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.248"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            154..477
                     /locus_tag="ACUQR0_004174"
     CDS             154..477
                     /locus_tag="ACUQR0_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HHV9110957.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="ACUQR0_004175"
     CDS             474..890
                     /locus_tag="ACUQR0_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HHV9110958.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="ACUQR0_004176"
     CDS             856..1380
                     /locus_tag="ACUQR0_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110959.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1598
                     /locus_tag="ACUQR0_004177"
     CDS             1377..>1598
                     /locus_tag="ACUQR0_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HHV9110960.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          389 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagc
//
LOCUS       DBKELA010000244         1587 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.249, whole genome shotgun sequence.
ACCESSION   DBKELA010000244 DBKELA010000000
VERSION     DBKELA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.249"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..626)
                     /gene="lpfB-O113"
                     /locus_tag="ACUQR0_004178"
     CDS             complement(<1..626)
                     /gene="lpfB-O113"
                     /locus_tag="ACUQR0_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HHV9110961.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1312)
                     /gene="lpfA-O113"
                     /locus_tag="ACUQR0_004179"
     CDS             complement(740..1312)
                     /gene="lpfA-O113"
                     /locus_tag="ACUQR0_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HHV9110962.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          473 a          333 c          315 g          466 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
     1321 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtatttttga
     1381 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
     1441 aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca agtttgttat
     1501 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
     1561 cagttataaa aaagggccac cggtcgg
//
LOCUS       DBKELA010000245         1566 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.250, whole genome shotgun sequence.
ACCESSION   DBKELA010000245 DBKELA010000000
VERSION     DBKELA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.250"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..81
                     /locus_tag="ACUQR0_004180"
     CDS             <1..81
                     /locus_tag="ACUQR0_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HHV9110963.1"
                     /translation="IRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(170..682)
                     /locus_tag="ACUQR0_004181"
     CDS             complement(170..682)
                     /locus_tag="ACUQR0_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HHV9110964.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            877..>1566
                     /locus_tag="ACUQR0_004182"
     CDS             877..>1566
                     /locus_tag="ACUQR0_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110965.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGID"
BASE COUNT          472 a          339 c          394 g          361 t
ORIGIN      
        1 atccgggacg gaatcatcag cgaccagaca gcattgagaa tgcttcagga gtacatcagg
       61 atccaatgcc ttggggggta gcggtaattt tactcattat ccttcaaatc agattctgtt
      121 gtcagaggaa tgggggaagc tggattcgca agagcttttt atgggcagtt tagctaaaaa
      181 aaggcaacac gaagctaaaa ctaccaacat aaagtgctga ccagctagca gctgaaatca
      241 gactcactgt atacactttc actggatgaa gttgcaacat tcctgccacc aaaggagcaa
      301 tgtaacgcag cacggcgata aagcgcgaag tgaaaagtat tgacacagag ttattttgca
      361 actgaaggcg aacccgttct attgtattgg aacggctcga tattatacat gcaagttgag
      421 ggatatgccc aattattata ccaagatggt aattaacgat agtaccgcac catgcgccac
      481 tcatgaccgt gattccagct tcccatggtg aaagagtcgt cttgctgact gtaattacag
      541 tggccatcat aattgatgct ggtggcaata tcgccgaaat gagtaaagtg gattttgtga
      601 acgctattat aaaaagaagc ccccataggc gatatggatg aagtacaaaa tagttcatca
      661 aagcatttat ccactccatc aatgtctttc cttctgtgaa tttttctttg ctatttaacc
      721 gtgatacgac ttaatttagg cttaaacacc ttacatttac aaggtactcc tgtggggggc
      781 cctgccacgg agcgtcgcag gagtgagtga tgaggaagaa ttaaagcaac ctattgtgac
      841 aggcagcgat aacagtaaat cacaggaagc ataatcatgg caaaaccgga ctgggaggcc
      901 atcgaatcgg cataccgggc cggagtcctt agcctccgtg atataggcga gaaatacggc
      961 gttacagaag gggctatcag gaagagggcc aaaaagcttg attgggcacg cagtggcggt
     1021 acgcaggttt gcaaaaatgg tacgcaaaaa aggaaagtgc gtaccagcag aaagcctgcc
     1081 attactggcc ttacacaaaa aagtacgcaa ctaaaaacag aatctacacc ggatacgaaa
     1141 ccgatacgcg gaatgcgtac cgatcccccg actaacccat tccaacccgg taaccagcag
     1201 gcattaaaac acggtggtta tgcccgtcgc cttctgctca aagatgaggt gatagaggac
     1261 gctaaagcgt tgacgctcga ggacgaatta tttcgccttc gtgctaacaa ccttgtcgct
     1321 gcagagaata ttggtcggtg gttggtgtcg ctggaagatg ctaatgggga ccaggaaagg
     1381 aagatgctga ttgaaaatat cagcgccgcc gagaaagcaa tgatgcgcaa tacagttcgt
     1441 attgagtcca tcgttggcac gcttgcgacg gtaggaaaaa tatttgctga tacagcctac
     1501 cgcaaggccg ccactgataa ggtgtctctg gaggctgatc gtcttcgccg tgatgcaggt
     1561 attgat
//
LOCUS       DBKELA010000246         1538 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.251, whole genome shotgun sequence.
ACCESSION   DBKELA010000246 DBKELA010000000
VERSION     DBKELA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.251"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(910..1386)
                     /locus_tag="ACUQR0_004183"
     CDS             complement(910..1386)
                     /locus_tag="ACUQR0_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHV9110966.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          409 c          385 g          396 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DBKELA010000247         1441 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.252, whole genome shotgun sequence.
ACCESSION   DBKELA010000247 DBKELA010000000
VERSION     DBKELA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.252"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..546)
                     /gene="ygeQ"
                     /locus_tag="ACUQR0_004184"
     CDS             complement(<1..546)
                     /gene="ygeQ"
                     /locus_tag="ACUQR0_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HHV9110967.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1201..>1441)
                     /locus_tag="ACUQR0_004185"
     CDS             complement(1201..>1441)
                     /locus_tag="ACUQR0_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110968.1"
                     /translation="GVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVV
                     DVLATGSVKVKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          422 a          306 c          300 g          413 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggaacctag gtatcgaggg tatcgaagat aatcagccgc taaccatttg tttccttaaa
      841 ggatgatgaa tccttgtggt tacagttatg gttacacatt aatttatctc tggtcaagtc
      901 ccgaagtcct aactttaaga cagtggcccc aatcgtccag acttgaaagg ggcctatggg
      961 ggaagtcgct gacctttatc tattaaatac ctacagagat ttttgtgaga ttttttaatc
     1021 caaggtcata acctactgcc cccacaggcc gcaaagtgaa gacctgtaag caccagagca
     1081 atagcgagta tctatcactc cctgtagagt atctggtaga ccaagaggac acagaggacg
     1141 accatataca actcctgtaa gctcatggat gtactcctta tgttcactca gggtttatag
     1201 ttactctgcg gcagtaaaac gaataacatg gaccaacggg aaagaggcgt cttcctcaac
     1261 gtcaaactca cctgctttca ccttaacgga accagtagcc aacacatcga ccacactgat
     1321 taactcaagg tcatacccag cagtgttgct atttcgggtc actttgacat ctaccgtctt
     1381 agcgtcttta gttgacctga aagagataga agcgaaccgc ttaggcagga gatagacacc
     1441 t
//
LOCUS       DBKELA010000248         1410 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.253, whole genome shotgun sequence.
ACCESSION   DBKELA010000248 DBKELA010000000
VERSION     DBKELA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.253"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(541..1350)
                     /locus_tag="ACUQR0_004186"
     CDS             complement(541..1350)
                     /locus_tag="ACUQR0_004186"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHV9110969.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKW"
BASE COUNT          471 a          280 c          268 g          391 t
ORIGIN      
        1 ctgcttatgc gtgttaatgt agtaaaaccc gagtggccta tgcatccaaa taaatagact
       61 tgttaatcta gggtaattgt tggaataata cgcatttttc aacaggtcca gataacctgg
      121 atataatgct cacttctaca atggcctcaa cttattttat ttaattgata ttacggaagt
      181 taactcatac ataaacaata tgactcagta ctgtactaaa tagctgcgtg gaaaagaact
      241 ccaccttcgc tcgtccttgc aggacgaggc atcagaacgg tgtccactgc caggcagtgt
      301 tatatctggc tttgatttat tcaggggctc gatggcccct ttattattca attcagggat
      361 aattttggcg agcttcgtgt aaggaatctt ttttcaatta gattccaaga aagatgagcc
      421 aagaaaagaa cgacaatggc acttaacaac atcgaagggt aaaagccaat atgcaatgta
      481 tttataataa cctgctgaac ggggaatgca taaatgtata caccataaga gtaatcaaac
      541 ctaccactta accagtggat ctccaaagga tgtgcatata gctattgttg agaaagaaac
      601 cagaatatag caagccattg tatagtcaat gcctttgcca tagctcgcgt atgcgtacat
      661 agcaagcaaa gaaatcacgg aaattttcac attacttaca ttccaagatt tctcatacat
      721 cgccatagtg gcaccaaaaa agaaagccat cccccgcaaa ggatacagcc atactggaat
      781 ggagaatatt acatccttgt tctcagatac actttcaact aatgaaagtg aaacaaaaat
      841 caatagtata actatgaacg tttttccgct ctttagtaga gcaacagcca ctccagttat
      901 tatgtaacac ataaactcca gtggtagtgt ccacaagctg ccgttaatac cgggatgaat
      961 taaatgtgat gtaatgtcgg catctggagc ctgactcatt aaaatagagc ttatggtctt
     1021 ccggataatg tcatgactaa aatattctgt actgaagttg ttaagcatcc acccaaataa
     1081 aaaatacgtc agtacagagc aagggacaag agcagggaat attcttcttg ctcttttagc
     1141 catgaaatca ataaaggaat cacttctgat tgctgatttt gaaatcaggt atcctgatat
     1201 ggaaaagaat atgataacag caatgccgcc agcactttca aagccgaata tatacggctc
     1261 gagttgtcca gagagcgcgt agtggtgact gattagaacc atcatagctg ccacaagcct
     1321 tgccgtatca aagcagttgc tcttatgcat taaaataaaa tccctaagtt tttgtgagga
     1381 aaatatcaga tctttatgtc atagtccatt
//
LOCUS       DBKELA010000249         1362 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.254, whole genome shotgun sequence.
ACCESSION   DBKELA010000249 DBKELA010000000
VERSION     DBKELA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.254"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..364)
                     /locus_tag="ACUQR0_004187"
     CDS             complement(<1..364)
                     /locus_tag="ACUQR0_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9110970.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(431..>1362)
                     /locus_tag="ACUQR0_004188"
     CDS             complement(431..>1362)
                     /locus_tag="ACUQR0_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HHV9110971.1"
                     /translation="IKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQ
                     QMGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFE
                     AVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQH
                     TVTPIAVGEVFNSIWDCKQLIEEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGS
                     HGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPG
                     LGISFDEKLAAKYPYDPEYLPVARLEDGTLLNW"
BASE COUNT          382 a          367 c          296 g          317 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aataattaag
      421 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatact
      481 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      541 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtacgggaaa acttcaagca
      601 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      661 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      721 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      781 tgatcgtcgt gcgaatatag ccaatcagct gctcttcgat taactgcttg cagtcccaga
      841 tactgttaaa cacttcaccg accgcaatcg gcgtcacggt atgctggcga atcagacgaa
      901 aacattcctg gttctcggca ggcgtcggat cttccatcca gaacaggcgg taatcctcaa
      961 tgctcttccc aaagcgcgcc gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca
     1021 gatgttcgtt aaaaccgaac ttattacgca ccgcttcgaa taacttcggc gtaaaatcga
     1081 gatacttttc tgaagaccac aactgctctt ctggccacag gcctttggtt gccggttcat
     1141 acgccagtcc tttgcccttc gccaggccat aagtggtttg catacccggt acaccgcact
     1201 gcacacgaat cgccttaaag cccatctgct ggtgctttgc atagtcttca aggacttcat
     1261 caatcgtacg cccggtggtg tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa
     1321 gcagttgata caatggcata tttgccgctt tagctttgat at
//
LOCUS       DBKELA010000250         1319 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.255, whole genome shotgun sequence.
ACCESSION   DBKELA010000250 DBKELA010000000
VERSION     DBKELA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.255"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..1270
                     /locus_tag="ACUQR0_004189"
     CDS             <1..1270
                     /locus_tag="ACUQR0_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HHV9110972.1"
                     /translation="NLSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVF
                     SKDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRL
                     MGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDA
                     MKKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGS
                     ENGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVT
                     LAYRPLNDTQRKIVDGLSGGLIDGLKMLAVAVTAQKAANVAGGLLNLANAVTSFGDAV
                     NKAKAGKAGSSGGALNKVGRVVLYAELVNFALDNLNPEAAKNPNLHQSGIGTETNFNP
                     EYKGDSSKPIVLSLTGLMVQLIKLLTSKNR"
BASE COUNT          400 a          238 c          305 g          376 t
ORIGIN      
        1 taatttaagt tcagcagcag ttaaaacagc aggtgtactt atcagctttc aggctgtact
       61 atccgcttat cagcgagtaa tggaagttgg ccttcaacgt gcaagtgctg aacgttcaat
      121 tgatttcgta ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca
      181 aacaacgggt ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc
      241 tagagagtct ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc
      301 tcgtctgatg gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat
      361 ggctggtaag ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc
      421 tggtatggtt caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc
      481 tatgaagaag ggggctttaa aatctgccga tactcttcag aaggtcactc aggaactgaa
      541 taaacaaatc accgctaaag gtggctggaa agctatttca gaaagtacac aggcacaact
      601 agggaacttg aaaaactctt ggaataccac gcttgatagt atcttcagag gttctgaaaa
      661 tggtctgcaa gattttacaa gaagcttaac caatctttta aacgctcttg gtggaagtgg
      721 taagagtctt ggtgaatctc ttggtagtct catgacttct atgtcacatg gtgtcgattc
      781 attgaccgat attagttaca gggtaagggc gttctttgat gaggtgacat tagcttaccg
      841 tccactaaat gacactcaga ggaaaattgt tgatggttta tctggtgggt taattgatgg
      901 tctgaagatg cttgctgtgg ctgttactgc tcaaaaggct gcaaatgtag caggaggctt
      961 gttaaacctt gcaaacgctg ttacttcatt cggtgacgct gttaataaag ctaaagctgg
     1021 caaggctggt tcttcaggtg gtgcgctaaa taaagtcggt agagttgtcc tttatgctga
     1081 gttggtaaat tttgctcttg ataatttaaa ccctgaagca gctaagaatc cgaacttaca
     1141 tcaatcaggt attggcacag aaacaaactt caaccctgaa tacaaaggtg attcatctaa
     1201 gccaatagtt ctatcgctga ctggtttaat ggtgcaacta ataaaattgc taacttccaa
     1261 gaaccgatga ataacttcgt aaataacaca caatcaactc catcagctat cacaggtga
//
LOCUS       DBKELA010000251         1230 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.257, whole genome shotgun sequence.
ACCESSION   DBKELA010000251 DBKELA010000000
VERSION     DBKELA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.257"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            50..460
                     /locus_tag="ACUQR0_004190"
                     /pseudo
     CDS             50..460
                     /locus_tag="ACUQR0_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(461..>1230)
                     /locus_tag="ACUQR0_004191"
     CDS             complement(461..>1230)
                     /locus_tag="ACUQR0_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHV9110973.1"
                     /translation="RLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTP
                     TGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGI
                     VEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFA
                     KELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          312 a          283 c          290 g          345 t
ORIGIN      
        1 tgctcattat tagctccttc tgatgccatt ctatttcagg aaggagtgtc cgttaaactc
       61 aggctacctc accctggtgc tggtggcaat tctgattatg ctgccgactc tcggcatcgg
      121 tgatatgaac aatctctata aatggctgtt gaatcttaac tcggtagtga tgccgctgcg
      181 ttatttatgg gtatttgttg catttattgc agtcgttcgc ctggcgcaga aatataaacc
      241 agaatatgtc tttattcgta ataagcctct ggcaatgacc gtcgggattt ggtgttttgc
      301 ctttaccgcc tgtgcctgtt tgacggggat cttcccgaaa atggaagcct tcactgcaga
      361 gtggaccttc cagttggcgc tgaatgttgc aacgccgttt gtgctggtag gattaggact
      421 gatattcccg ctgctggcgc gtaaagcgaa tagcaaataa ttattgtggt cggtgacgct
      481 gactctggca gatctgaccc gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca
      541 tatactggcg gtttgggtaa tagacttgga agcctggacg ctctgtgatc cagtcttcca
      601 gtacacattc taaacgacca gtatcaaggt actccttgat ctgttcatag agcagatacc
      661 cgataccagc acccatcaaa acggcctcca gttcagcatc aacatcatcc agaatgatat
      721 ttcctgcgac agcaatttcc agctcctttg caaattgcca gtggaaaggt ttgccgctgg
      781 gataacgaaa aacaacacat tgatgattaa gcagatcatg aggatggcgg ggtttgccat
      841 atcttgcaaa gtactctggc attgctgcta cacataattt cactggtggg ccgatcgcca
      901 cgctgatcat atctttttcg acgatgccac taagtcgaac gccagcgtca aagccctgtt
      961 ggacgatatc gataagagaa tcgtcagttg tgagctctac tttaatatca ggatattcac
     1021 gtgtgaaacc aaccagtaaa ggcattaaaa atatacgtgc agctacccgg gcggcattga
     1081 ttttgagcgt tcctgtcggt gtcagacgaa aatcattcat ctcatctaac atgatctgaa
     1141 tttcgtcgaa agctgggcgc aatcgttcat agagattaga acccgcttcc gttaacgata
     1201 cgcttcgggt tgtccgattg aataagcgaa
//
LOCUS       DBKELA010000252         1096 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.258, whole genome shotgun sequence.
ACCESSION   DBKELA010000252 DBKELA010000000
VERSION     DBKELA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.258"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(137..760)
                     /locus_tag="ACUQR0_004192"
     CDS             complement(137..760)
                     /locus_tag="ACUQR0_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HHV9110974.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
     gene            complement(711..>1096)
                     /locus_tag="ACUQR0_004193"
     CDS             complement(711..>1096)
                     /locus_tag="ACUQR0_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110975.1"
                     /translation="YWPISRLTDWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIA
                     AYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPG
                     SYCALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          225 a          333 c          260 g          278 t
ORIGIN      
        1 gaggcccgcc cccgggcctc cccggattca tccgtttctc tgttcagcct gaccgcacac
       61 cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag cggtcttctg tgattccgcc
      121 agttgcggct tattcattac ttaacgtcaa acgcccgaat tgaagccaaa tcatccagac
      181 tgctcagctc ctctttcatc tcccgctgac ggcgataaat ctcatcgttg cgatcgacct
      241 gcgcctgcac cattgctgcc gccagttctt ccagttccgg catcgacagt ttcacctgct
      301 gattatccgc atcactccac accatatgtg tttgtgccgc gacagatttt gccagcatga
      361 ccaccgggga caggcgggca actgacgagg cgtcagcgtt ccacgtgcgg ccgttccatt
      421 caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc ttcaatttcc tgacgtctgg
      481 cctctctggc cgcttccagc atttctggtg tcacagtgaa tggggctatc tcaccccatt
      541 tgccactttg cagttcctgc cagatttgct gacccgtcgg tgcaacatca tcagcggtgg
      601 ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac gtcacagtct actgcgccac
      661 tttcggtata acggggatta atgatttttt taatttccac ggtgcattcc tcacgatgtg
      721 cgaataaaaa gcccgggcat tgcgccagag acatgagcat ccggcacccc ggacagggcg
      781 caatatgacc ctggtaatga atgctctgaa catcccgtaa tgaaaaattg tggggatgct
      841 atatacgttc cggtgggagt actgggcact gaaatcccca ccggtcccag tcgtgagcct
      901 ctgtatgact gccccctgac aagtctgatg actttatcac cgtcagcttc tccctggtac
      961 gcagcaataa tcagcccgcc aatgtcaggg tctccccatc tgttgcggac agagctcgcc
     1021 acgattctgt aaataatatc ttctgtggtt atatttattt tcacccagtc agtcagtctg
     1081 gatatgggcc agtagc
//
LOCUS       DBKELA010000253         1087 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.259, whole genome shotgun sequence.
ACCESSION   DBKELA010000253 DBKELA010000000
VERSION     DBKELA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.259"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            37..>1087
                     /locus_tag="ACUQR0_004194"
     CDS             join(37..286,286..>1087)
                     /locus_tag="ACUQR0_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HHV9110976.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
BASE COUNT          298 a          268 c          306 g          215 t
ORIGIN      
        1 gtgatatgct cacctcagaa caacacaggt gctccaatga aaaaaagaaa ttttagcgca
       61 gagtttaaac gcgaatccgc tcaactggtt gttgaccaga aatacacggt ggcagatgcc
      121 gccaaagcta tggatgttgg cctttccaca atgacaagat gggtcaaaca actgcgtgat
      181 gagcgtcagg gcaaaacacc aaaagcctcc cccattaccc cggaacaaat tgaaatccgt
      241 gagctcagga aaaagctaca acgcattgaa atggagaatg aaatattaaa aaaggctacc
      301 gcgctcttga tgtcagactc cctgaacagt tctcgataat cgggaaactc agagcgcatt
      361 atcctgtggt cacactctgc catgtgttcg gggttcatcg cagcagctac agatactgga
      421 aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt acgcagtcag gtacttgagc
      481 tacatggcat cagccacggt tcggccggag caagaagcat cgccacaatg gcaacccgga
      541 gaggctacca gatgggacgc tggcttgctg gcaggctcat gaaagagctg gggctggtca
      601 gctgtcagca gccgactcac cggtataaac gtggtggtca tgaacatgtt gctatcccta
      661 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
      721 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
      781 gaaaaccagt gggctgggcc atgtcgttct cgccggacag caggctcacc atgaaagcgc
      841 tggaaatggc atgggaaacc cgtggtaagc ccggcggggt gatgttccac agcgatcagg
      901 gcagtcatta tacgagcagg cagttccggc agttattgtg gcgataccag atcagacaga
      961 gtatgagccg gcgcggaaac tgctgggata acagcccaat ggaacgcttc ttcaggagtc
     1021 tgaagaacga atggatgccg gtggtgggtt acgtaagctt cagcgaggca gctcacgcca
     1081 taacgga
//
LOCUS       DBKELA010000254         1085 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.260, whole genome shotgun sequence.
ACCESSION   DBKELA010000254 DBKELA010000000
VERSION     DBKELA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.260"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            472..>1085
                     /gene="uacT"
                     /locus_tag="ACUQR0_004195"
     CDS             472..>1085
                     /gene="uacT"
                     /locus_tag="ACUQR0_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HHV9110977.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKG"
BASE COUNT          260 a          250 c          234 g          341 t
ORIGIN      
        1 acattccgca gcttcagcag cgataaactt attcatttgc atccctttca tttaatgagt
       61 tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc atgaccgttt
      121 taattataat tttgcgactg agttcaaatt attgcccctc aatctatgcc gctttcatta
      181 tttcaacatt gccgatcaaa agagcgctat ccagataaaa acgattaaat gcgagagtgc
      241 gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa ttccattgtc
      301 aggttctctt gtcattttcg tggtttaagc acgattatgt gattgcctcc gcaattcctg
      361 tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt agacagtttc
      421 attaacgtaa acggttgctt tttattctgg cgggcgaaag gagaaacact gatgagcgcc
      481 atagattccc aacttccctc atcttctggg caagaccgcc caactgatga ggttgaccgc
      541 atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt catgtacgca
      601 ggtgcagtcg ctgttcctct tatgattggt gaccgactgg gcctctcaaa agatgctatt
      661 gcgatgctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt gcaatgtatc
      721 ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac ctttgctgct
      781 gtaacaccaa tgatagccat tgggatgaac ccggatatcg gcctgctggg gatatttggt
      841 gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttattgg tcgcttgatg
      901 cctttattcc cgccactggt taccggtgtg gttattactt ctattgggct tagcatcatt
      961 caggtgggta ttgactgggc cgctggaggt aaagggaatc cgcaatatgg taatcccgtt
     1021 tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg ctatgcgaaa
     1081 ggatt
//
LOCUS       DBKELA010000255         1072 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.261, whole genome shotgun sequence.
ACCESSION   DBKELA010000255 DBKELA010000000
VERSION     DBKELA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.261"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..563
                     /locus_tag="ACUQR0_004196"
     CDS             <1..563
                     /locus_tag="ACUQR0_004196"
                     /inference="COORDINATES: protein motif:HMM:NF024120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HHV9110978.1"
                     /translation="NTKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTD
                     NYQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAK
                     SGLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVM
                     VNEVETALFNARALALKIYSMSGWNL"
     gene            790..>1072
                     /locus_tag="ACUQR0_004197"
     CDS             790..>1072
                     /locus_tag="ACUQR0_004197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HHV9110979.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          316 a          202 c          258 g          296 t
ORIGIN      
        1 ataacaccaa aggcgatgcc atgattggcg tcaagcagcc tttaccaaaa gcagttttaa
       61 gaactcagca tgacaaaaat aaagaagcaa taagtatcct ggattttggt gttattgatg
      121 atggtgtgac agataattac caggcaatac aaaatgctat agatgccgtt gcttcactac
      181 cctccggcgg ggagctgttt atccctgcga gcaaccaagc ggtggggtat attgttggat
      241 ccactttgct tattcctggc ggtgttaaca tcagaggagt tggtaaggca tcgcaactcc
      301 gagcaaaaag cggacttaca ggatctgtgt taaggctgtc ttatgattca gacactatcg
      361 gccgttatct gagaaatata cgagtaactg gtaataacac ctgcaatggt attgacacaa
      421 acattacagc agaagactct gtcatcagac aggtttatgg ctgggtattt gataatgtaa
      481 tggtgaatga agttgaaacc gcattattta atgcaagggc tctggcactc aaaatttata
      541 gcatgtcagg ctggaacctg tagagtcggt cttcactttt taggccagtg cgtaagtgtt
      601 agtgtcagct cctgccattt cagcagagga aattattctg ctgatgaaag ctttggcatc
      661 aggattcagc ctcaaacgta tgcgtggtcg tcagaggcag ttaggtcaga agcaataatt
      721 ttagacagtg agaccatgtg cattggtttt aaaaatgccg tctatgttca tgattgcctt
      781 gatttgcata tggaacaact ggatttagat tattgcggct caacaggcgt ggttatagag
      841 aatgtaaacg gaggattttc tttctcaaac tcatggatag cagcagatgc cgatggcact
      901 gaacaattta cggggatata ttttagaacg ccggcctcaa cgcagtcaca taaaattgtc
      961 agtggtgttc atatcaacac tgcaaacaaa aacacggctg caaacaatca aagtatagcg
     1021 atagaacagt cggcgatctt cgtctttgta agtggttgta cgttaactgg tg
//
LOCUS       DBKELA010000256         1066 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.262, whole genome shotgun sequence.
ACCESSION   DBKELA010000256 DBKELA010000000
VERSION     DBKELA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.262"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(570..749)
                     /locus_tag="ACUQR0_004198"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACUQR0_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACUQR0_004199"
     CDS             843..>1066
                     /locus_tag="ACUQR0_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHV9110980.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DBKELA010000257         1032 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.263, whole genome shotgun sequence.
ACCESSION   DBKELA010000257 DBKELA010000000
VERSION     DBKELA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.263"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..300)
                     /gene="tnpB"
                     /locus_tag="ACUQR0_004200"
     CDS             complement(<1..300)
                     /gene="tnpB"
                     /locus_tag="ACUQR0_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HHV9110981.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLAGDPGRESAPDASSVIHATGGDRVATSQT"
     gene            complement(297..971)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_004201"
     CDS             complement(297..971)
                     /gene="tnpA"
                     /locus_tag="ACUQR0_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HHV9110982.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
BASE COUNT          236 a          265 c          268 g          263 t
ORIGIN      
        1 cgtttgggat gttgccacgc gatcccctcc agtagcatgg ataactgagc tggcgtcagg
       61 tgcactttcc cttcccgggt caccggccag acgtttggcg aacaggcata acccgtcacg
      121 atcggcccac agtattttca ccattttgcc actgcggccc cggaagacga agatatgccc
      181 ggagaacggg tcatctttca gcgtgttctg caccttcgaa gccaggccat tgaagccaca
      241 acgcatatct gtgatgccag cgatgatcca gattttggta ccggtcggca gcgttatcat
      301 cggatacccc ctttcatttc gcggattagc gcccgtaaca gttccggagt gagagggtca
      361 aacagtttta ccacacctga tttaagatgc agctcgcacc gtgggacgtt tccgggagcc
      421 ccctcagggc gctcatcatg cttgttacgc cagaagggat ttgtaactgg tctggtcggc
      481 tccggcgtat cagtcagtgc caccgggaca ggcatgcatt cctgtatgtc atcatcgctc
      541 agtaagccgt cctcgtactg gcttttccat ttaaacagca ggttattatt gatatcgtgt
      601 tctctggcga tccgggcaac aacagcccca ggctgtaatg cctgcttagc cagacggacc
      661 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
      721 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      781 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      841 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      901 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      961 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
     1021 attttcacaa ga
//
LOCUS       DBKELA010000258         1031 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.264, whole genome shotgun sequence.
ACCESSION   DBKELA010000258 DBKELA010000000
VERSION     DBKELA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.264"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>1031
                     /locus_tag="ACUQR0_004202"
     CDS             <1..>1031
                     /locus_tag="ACUQR0_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HHV9110983.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAE"
BASE COUNT          319 a          193 c          270 g          249 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag agcggggata aaggattagt
      901 aaccttaaaa cagaaagagg gtaaacccgt ccccttcggc gctgtgatta gttatagcaa
      961 agacactgaa aatatggccg gcattgtagg tgaagatggt attgcttatg tttctggctt
     1021 gagtgcggaa g
//
LOCUS       DBKELA010000259         1026 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.265, whole genome shotgun sequence.
ACCESSION   DBKELA010000259 DBKELA010000000
VERSION     DBKELA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.265"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(56..196)
                     /gene="yqcG"
                     /locus_tag="ACUQR0_004203"
     CDS             complement(56..196)
                     /gene="yqcG"
                     /locus_tag="ACUQR0_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HHV9110984.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            335..1006
                     /gene="queE"
                     /locus_tag="ACUQR0_004204"
     CDS             335..1006
                     /gene="queE"
                     /locus_tag="ACUQR0_004204"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HHV9110985.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          300 a          206 c          239 g          281 t
ORIGIN      
        1 aaaggaacat acgaaagtga acattagtat gaaatagcgc attataaatt tctcattaaa
       61 tctggataaa taaattgacg gcggcatcgg taattttaga ttcgttatca ttaaaaacta
      121 atacagaaaa aataaatata attttggtga atgttttgac atgattaaat ccattttctt
      181 tattttcttc tgacataata cgttactccc tttaattatt aacgatatgt ttcgtatttt
      241 tctttaaatc tgcatgtaac agaatgttaa taagacatga ttgttgcggc aggatctgca
      301 ataattaaca attatccact tcacagagaa tgctatgcag tacccgatta acgagatgtt
      361 ccagaccctg caaggtgagg gttactttac cggcgtcccc gccattttta ttcgtttaca
      421 gggatgcccg gttggctgtg cctggtgcga caccaaacac acctgggaaa agcttgagga
      481 tcgggaagtc tcccttttca gcattctggc gaagaccaaa gagagtgata agtggggggc
      541 ggcgagcagt gaagacttgc tggctgtcat tggtcgtcag ggatataccg cgcggcatgt
      601 ggtgattacg ggtggtgagc cttgcattca tgatttgctg ccactgactg acctgctcga
      661 aaagaacggt tttagctgcc agatcgaaac cagtggaact catgaagtac gctgcacacc
      721 gaatacctgg gttaccgtat cgccaaagct gaacatgcgc ggcggctatg aagtgttgtc
      781 acaggcactg gagcgagcca acgaaatcaa gcatccagtg gggcgcgtac gcgatattga
      841 agcactggat gaactactgg caacgctgac cgatgataaa ccgcgagtca ttgcactgca
      901 gccgattagc caaaaggatg atgccacacg attgtgcatt gaaacctgta ttgcgcgtaa
      961 ttggcgtttg tcgatgcaaa cacataaata tttaaatatt gcctgattac acatttataa
     1021 gcgtta
//
LOCUS       DBKELA010000260          983 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.266, whole genome shotgun sequence.
ACCESSION   DBKELA010000260 DBKELA010000000
VERSION     DBKELA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.266"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACUQR0_004205"
                     /pseudo
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACUQR0_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT          260 a          220 c          221 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctaaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DBKELA010000261          969 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.267, whole genome shotgun sequence.
ACCESSION   DBKELA010000261 DBKELA010000000
VERSION     DBKELA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.267"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..932)
                     /locus_tag="ACUQR0_004206"
     CDS             complement(<1..932)
                     /locus_tag="ACUQR0_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HHV9110986.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLAPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMA"
BASE COUNT          198 a          266 c          276 g          229 t
ORIGIN      
        1 tccgccattt ctcctccggg gaagcgcatg gcgtccgtca tcgacgtcag cagatggcag
       61 acttttcctt tgcgcgtcac ggtcagcagg cgggctgtca cttcatttcc cagtcccggc
      121 cacttttttc gtgcctgcgg gctggttttc agcttcacca gatgatcgcc tttacccagt
      181 tttctgagct cttcatattg cgctcccttt ctgagaggta tcatccagtg gcggtgttct
      241 cccgccaggc tccagccatt taacagtccc agtgagtaat aacctttatc cattaacgtc
      301 agagtgttat cgccggtttg ttctataagt tgctcagcaa gctcattttc gctgttcttc
      361 atcgtgccga aggctgcagc cgtcagcaga tggctggtca gttccatctg gcagaccatt
      421 ttgacctgcg ggtagagcgc cgggttcccg gcatgtgtct ggcgggggaa ggctgcatcg
      481 ttctctggtg tatccggtgt gcgccagaac acaccatcga tggccagcag ggtcaggccg
      541 caccagtgcg gatgcggcgt ggcgttatgc cagagctgcg ctgttttcgt gaacacgcgg
      601 cggacagcct cacttcccag gcgctggcgg gcctgaataa cggcactggg ggcaacgaag
      661 gggcgattgc ccggcagcat gatgtccagg cgattcacaa tctggtgaag aggttcttta
      721 cgctcaagcg ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg
      781 cgtagcgtta cagtacctga ttcggcaagg caacgagaga tgagttcggg ggcgaggtaa
      841 tcccccagag aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc
      901 tgtccaatgt gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc
      961 actggatcg
//
LOCUS       DBKELA010000262          933 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.268, whole genome shotgun sequence.
ACCESSION   DBKELA010000262 DBKELA010000000
VERSION     DBKELA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.268"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(55..243)
                     /locus_tag="ACUQR0_004207"
     CDS             complement(55..243)
                     /locus_tag="ACUQR0_004207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9110987.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACUQR0_004208"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACUQR0_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DBKELA010000263          915 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.269, whole genome shotgun sequence.
ACCESSION   DBKELA010000263 DBKELA010000000
VERSION     DBKELA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.269"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(509..>915)
                     /locus_tag="ACUQR0_004209"
     CDS             complement(509..>915)
                     /locus_tag="ACUQR0_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9110988.1"
                     /translation="IKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDG
                     LRLMKNGYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIM
                     GHSSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          264 a          219 c          176 g          256 t
ORIGIN      
        1 ggggtaatgc ctagaggaga ccctagagag acctatagag agaccctaga gaagacctat
       61 aggtttggtg attgattatt ataaatatca tcacccctta aggtattccc ttagagttaa
      121 tctttaattg gtctttaatt aaatctttta tttaatactt aaagttaatc tctaattgtg
      181 tctttaagtt aactttaagt taagcccctg tagggactag agaccataat taagatgatt
      241 gtgtcttatt gctattcact tatcgactga cccagagcac acaggaagca ttcactttaa
      301 gtagaacgct tggtgtctgt aattggtcat cccgcccctg aaaggatgcg ataagttacg
      361 gttagttgtc ctagaagaga aaactttttg tgtcccttct ccctatagtg agtcgtatta
      421 attatccctt gataagtaga gaaaactatg agtcaaaaac atgaccacac aggtctgtag
      481 taagtgtcat cctagactat ggtctcctct aggtctcctt ataaacatca gccatcacat
      541 tgaccagcct atcaacctcg acctctttag cataacggtc gtacgtaata gaccctgaac
      601 tatgccccat gatcgcctgt gcatacgcca actcgacccc atgttgtttt aactgggtcg
      661 caactgtatg tctaaacgag tgaaacgcta agccgctacg gtctacgagg actttaggca
      721 acaacgtccg attaaaccat ttactcacct gctcaccgta accgttcttc atgagacgca
      781 gaccatcgaa caccatagcg tcatcaccgt cagcgcctct acgggtctct acaagcgcca
      841 taaagtcagc caatataaaa ccataggccc catcgaccag aggcacacag cggtcactat
      901 gagcgttctt gatgc
//
LOCUS       DBKELA010000264          898 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.270, whole genome shotgun sequence.
ACCESSION   DBKELA010000264 DBKELA010000000
VERSION     DBKELA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.270"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACUQR0_004210"
     CDS             complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACUQR0_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HHV9110989.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="ACUQR0_004211"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="ACUQR0_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HHV9110990.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="ACUQR0_004212"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="ACUQR0_004212"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HHV9110991.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          253 a          180 c          232 g          233 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgt
//
LOCUS       DBKELA010000265          813 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.271, whole genome shotgun sequence.
ACCESSION   DBKELA010000265 DBKELA010000000
VERSION     DBKELA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.271"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..769
                     /locus_tag="ACUQR0_004213"
     CDS             <1..769
                     /locus_tag="ACUQR0_004213"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9110992.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
BASE COUNT          271 a          178 c          170 g          194 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtc
//
LOCUS       DBKELA010000266          812 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.272, whole genome shotgun sequence.
ACCESSION   DBKELA010000266 DBKELA010000000
VERSION     DBKELA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.272"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..366)
                     /locus_tag="ACUQR0_004214"
     CDS             complement(<1..366)
                     /locus_tag="ACUQR0_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HHV9110993.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEG"
     gene            complement(378..779)
                     /gene="gpFI"
                     /locus_tag="ACUQR0_004215"
     CDS             complement(378..779)
                     /gene="gpFI"
                     /locus_tag="ACUQR0_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HHV9110994.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          200 a          252 c          168 g          192 t
ORIGIN      
        1 cccttcataa tatcgtcctg cccttacgtt tcccggcagc ccctcacgcc gcaaccggat
       61 ataactacat ccgccatcat caggggtgat acgatccaca cagaaagact cacgaccaat
      121 catcagcgaa tcattgcgac acagccgtaa aatatctgct gttttcacaa acagggatgg
      181 taaagagtct tcaatacgca caccgccggc caccagagaa atattttcag gatcatcaaa
      241 aatcccggaa atcatgatcc cttttagccg gcctgacgta atgaccgctc taatccccat
      301 attgcgaata atggccttgt ctgcaagatc aagtgcaaca tcaaacagat tatcgaaatc
      361 agccacgctg cctcccgtta ttgcattctg gccaggccac gttctgtcat tccggctgcc
      421 acaccggcaa agacacggaa cgccattccc ggcggcacaa atgccagagg ttcatcccgc
      481 gtggcatgaa gtgcatcggt atgcagcgtc accagtgcca cgactgtaac cagatcatcc
      541 atattctgaa tcacggtctc cggctgcgct gatatcacct cattttcatg tccggtcagc
      601 gcattttccg ggtgggcaga cttatcccgg atgtcagtgt catccatatt ggcaagttcc
      661 tcctccagct cagccacacg ctgcgcaata tctgctgcgc ttcccgagat atccggctca
      721 cgtcccagcg atgctgccag ttctctcaga cgtttcaggt tatcgtcttt ctttgccata
      781 tctgttcctg tccgaataaa aaacgggagc cc
//
LOCUS       DBKELA010000267          772 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.273, whole genome shotgun sequence.
ACCESSION   DBKELA010000267 DBKELA010000000
VERSION     DBKELA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.273"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            373..579
                     /locus_tag="ACUQR0_004216"
                     /pseudo
     CDS             373..579
                     /locus_tag="ACUQR0_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT          211 a          134 c          205 g          222 t
ORIGIN      
        1 tgttgttaat aaaaagctta tagatttata gttgataatt aaaacgcatc cgatatttca
       61 gctttttcta tgcaagatac aattttataa ggtgtccatt tttatatgga ggcggtagcg
      121 tgtctgttac tagtggtttt atttgcatag tgaatatatt gggattgtta aatacataac
      181 tatgtatccg ctctggtcgt tgtaaatatt gctaattgct taatattatt agtgattatg
      241 gctggaaaga taaagtgtgc tgtaaagcgg tgatgggtat tacattcatg attaaaaaaa
      301 cagaacctgt caataaataa ttcctttatt aaaacggact atttacatta tgacggaata
      361 ttatctcaac gaggcggttt ggaaatacag agccagtttc tccggggtaa cgagcaggtg
      421 taccagtgtc aggtggcatt tgtattgccg ggtgagcggg tcatgatggc cttcacctac
      481 gccagaacca caccgctgac cccggcagat atgacgcgct gggctgaaat taaaaagaat
      541 ctgcgcttca ggatgcggca ggaagtcagg acgaattaat catacaggga acgggacatg
      601 gaagaggcat tctgggcggc aagacagggt gatgccctgc tgcatacgtc gtttatggca
      661 gatgtgctgg gggcggtgct ggaggtggcc gcaaatgtcg ttattgatgc cctcattgtc
      721 ggcacaacct cgttactggc gacgtcagtg gggatcacga tggggtgcac gg
//
LOCUS       DBKELA010000268          756 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.274, whole genome shotgun sequence.
ACCESSION   DBKELA010000268 DBKELA010000000
VERSION     DBKELA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.274"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..756
                     /locus_tag="ACUQR0_004217"
                     /pseudo
     CDS             1..756
                     /locus_tag="ACUQR0_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          233 a          162 c          178 g          183 t
ORIGIN      
        1 tatcagttga tccagaaaaa gcggcatcag cattggagct gtcaaaagac cgacgcattg
       61 aggatcttga aaggcaagtt cgatttcttg aaacgcggct tatgtatcta aaaaagctga
      121 aagccttagc tcatcccacg aaaaagtgaa agtactcaac gagctaaggc agttttatcc
      181 tcttgatgag cttctcaggg ctgcggagat accgcgcagt acgttttatt accatctaaa
      241 ggctctcagc aagcctgaca agtatgcgga cgttaaaaag cgtattggtg agatttatca
      301 cgagaataga ggccgatacg gataccgtag ggtaacgctg tctcttcatc gagaagggaa
      361 acagattaac cataaagctg ttcagcgcct gatgggaacc ctctcactta aagcagcgat
      421 taaggtcaag cgataccgct cttacagagg agaggtaggg caaaccgccc ctaatgttct
      481 ccaaagagat ttcaaggcta cgcggccaaa cgagaagtgg gttaccgatg ttactgaatt
      541 tgcagtcaat gggcgcaagc tgtatttgtc tccagtaata gatctcttca acaacgaagt
      601 tatttcttac agcctttcgg aaagaccagt gatgaacatg gttgagaata tgctcgatca
      661 ggcattcaaa aagcttaatc ctcacgagca tcctgttctg cactctgacc agggatggca
      721 gtatcgtatg agaagatatc aaaatatcct taaaga
//
LOCUS       DBKELA010000269          755 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.275, whole genome shotgun sequence.
ACCESSION   DBKELA010000269 DBKELA010000000
VERSION     DBKELA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.275"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(50..126)
                     /locus_tag="ACUQR0_004218"
     tRNA            complement(50..126)
                     /locus_tag="ACUQR0_004218"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Ile,seq:cat)
     gene            complement(316..>755)
                     /locus_tag="ACUQR0_004219"
     CDS             complement(316..>755)
                     /locus_tag="ACUQR0_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HHV9110995.1"
                     /translation="GKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRD
                     MINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELLN
                     DAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          191 a          204 c          145 g          215 t
ORIGIN      
        1 accgtcaact tagaaggttg atgctctatc cggatgagct agtggtggtt ggtggccctt
       61 gctggacttg aaccagcgac ctggcgatta tgagtcgctc gctctgacca actgagctaa
      121 agggccggag gcagaataat aaccatatgt catcacatct gcaaactcat ctgatcacca
      181 gcacgtttaa cgtcctgtgc cgtttttcag gtataaaaaa aaccgcataa agcgggctct
      241 ttcaaatgtc catgtctgct attcgcctcg cggtacagct ttgcgaagcg tacaagaatt
      301 gaagcagttt ttacgtcaaa aagcaataac ttttttctct ataccaaaag ccataaccat
      361 tggtttgtac aaaataaatt ctgccacctt tagccaatgc tcaatgcgtc tttcacaggt
      421 tcttaaactc catttcgggt gtgcatcatt cagcagttca gccattttgc atttggtcat
      481 cccccgcccc tcataccgtt gccgaaggac gctaatcaat cctggatgct ctgccagcac
      541 ctcacttatg acccgatcaa tacataacgc ctctgcatca gtacaatgcg ccagccagct
      601 cttttgctta ccgttaatca tatcccgcaa aaaagcctca agttcagact tgttcagacc
      661 tgcttttttc atcctccgga gcgcctcgtt aattgccgtt tttgtcagct ttttagaggt
      721 caacatcagg ttaaacatat tccccgtctt accgc
//
LOCUS       DBKELA010000270          748 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.276, whole genome shotgun sequence.
ACCESSION   DBKELA010000270 DBKELA010000000
VERSION     DBKELA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.276"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(62..138)
                     /locus_tag="ACUQR0_004220"
     tRNA            complement(62..138)
                     /locus_tag="ACUQR0_004220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Arg,seq:tct)
     gene            complement(140..215)
                     /locus_tag="ACUQR0_004221"
     tRNA            complement(140..215)
                     /locus_tag="ACUQR0_004221"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Ile,seq:cat)
     gene            complement(392..>748)
                     /locus_tag="ACUQR0_004222"
     CDS             complement(392..>748)
                     /locus_tag="ACUQR0_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HHV9110996.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          182 a          196 c          155 g          215 t
ORIGIN      
        1 atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc taagtcggcg
       61 ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg atgctctatc
      121 cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac ctggcgatta
      181 tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata accatatgtc
      241 atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc atttttcagg
      301 cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac aaaatcggca
      361 aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata ctttgctttg
      421 aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc tcactatcca
      481 gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg gaccagtttt
      541 tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc ccaccaaaaa
      601 gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt tcctgtgttt
      661 tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc cagtgggtta
      721 tggtgatctg atactcccag caaatact
//
LOCUS       DBKELA010000271          747 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.277, whole genome shotgun sequence.
ACCESSION   DBKELA010000271 DBKELA010000000
VERSION     DBKELA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.277"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>747
                     /locus_tag="ACUQR0_004223"
     CDS             <1..>747
                     /locus_tag="ACUQR0_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HHV9110997.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DBKELA010000272          722 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.278, whole genome shotgun sequence.
ACCESSION   DBKELA010000272 DBKELA010000000
VERSION     DBKELA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.278"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>722
                     /locus_tag="ACUQR0_004224"
     CDS             <1..>722
                     /locus_tag="ACUQR0_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HHV9110998.1"
                     /translation="FDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDPKTDLSAVTLP
                     RALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLEKGAEYVRL
                     DAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPHKDNIAYFGNG
                     DDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFLASHDGIGLNP
                     LRGLLPESEILALVEALQQEGA"
BASE COUNT          181 a          167 c          178 g          196 t
ORIGIN      
        1 tttgattttg tctgcaacca tatgtctgca aaaagtgaat ggtttaaaaa ctatttacaa
       61 cagcttccgg gttttgaaga tttctttatt gcggttgatc cgaaaaccga tctcagtgcc
      121 gtcacccttc cgcgtgcgtt accgctatta acgccgtttc aaatggatga taattctacg
      181 cgccatttat ggaccacctt tagtgacgat caaattgacc tgaattaccg tagccctgaa
      241 gtgttgctgg cgatggtgga tgttttactc tgttaccttg agaaaggtgc tgagtatgtc
      301 cgcctggatg ccgttggctt tatgtggaaa gagccgggaa caagctgcat ccatctggaa
      361 aaaacacatc tgattatcaa gctgttacgg tcgattattg atgacgtagc accaggtaca
      421 gtgatcatta ccgaaaccaa tgttccgcac aaagacaaca ttgcttactt tggcaacggt
      481 gatgacgaag cgcatatggt gtaccagttc tcgctgccgc cgctggtgtt acatgcggtg
      541 caaaaacaga acgtcgaggc gctgtgccag tgggcgcaaa gtctgagctt gccctccagt
      601 aaaacgacct ggtttaactt cctcgcctct cacgatggca tcgggttgaa cccactgcgt
      661 ggcttgcttc cagaaagcga aatattagcg ctggtcgagg cattacagca ggaaggtgca
      721 tt
//
LOCUS       DBKELA010000273          652 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.279, whole genome shotgun sequence.
ACCESSION   DBKELA010000273 DBKELA010000000
VERSION     DBKELA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.279"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..367)
                     /locus_tag="ACUQR0_004225"
     CDS             complement(<1..367)
                     /locus_tag="ACUQR0_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HHV9110999.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGEC"
     gene            complement(579..>652)
                     /locus_tag="ACUQR0_004226"
     CDS             complement(579..>652)
                     /locus_tag="ACUQR0_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="rhodanese; catalyzes the formation of thiocyanate
                     from thiosulfate and hydrogen cyanide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HHV9111000.1"
                     /translation="EMGYTHVENAGGLKDIAMPKVKG"
BASE COUNT          186 a          136 c          139 g          191 t
ORIGIN      
        1 tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat
       61 aatcaattac cgaaaatcca tcatcagatg accatgggta aaaaggtaat aaatgaacat
      121 gtgaaaaaat gctttgcagc cattgatggt agaactgatt aaatatgggt agtggtttta
      181 aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt tcatcccagt
      241 gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct
      301 gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc catgtaatcc gtaattttct
      361 gtttcatgtt gcctttccgt ggtagcgatc ccatgaggcg agagactact ccgcagaaaa
      421 taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt taccagggaa
      481 aaagcgtatt tgtgagctga cgcaaataat ctatagggat gtaaataagg tgtgacagaa
      541 aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac cttcggcatt
      601 gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc gc
//
LOCUS       DBKELA010000274          630 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.280, whole genome shotgun sequence.
ACCESSION   DBKELA010000274 DBKELA010000000
VERSION     DBKELA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.280"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..112)
                     /locus_tag="ACUQR0_004227"
     CDS             complement(<1..112)
                     /locus_tag="ACUQR0_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HHV9111001.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="ACUQR0_004228"
     CDS             complement(109..399)
                     /locus_tag="ACUQR0_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HHV9111002.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>630)
                     /locus_tag="ACUQR0_004229"
     CDS             complement(399..>630)
                     /locus_tag="ACUQR0_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HHV9111003.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          149 a          181 c          136 g          164 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc acgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg
      301 gtaatgcgcc agcacagacg tttccggatc gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttaagtaa atccaccatt acgcaaactc cagcagctgc
      421 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacagaat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat attcgcaaac
      541 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
      601 ccggcctgaa tggctaccca ctcgcggaaa
//
LOCUS       DBKELA010000275          626 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.281, whole genome shotgun sequence.
ACCESSION   DBKELA010000275 DBKELA010000000
VERSION     DBKELA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.281"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            88..153
                     /locus_tag="ACUQR0_004230"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACUQR0_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..>626
                     /locus_tag="ACUQR0_004231"
     CDS             381..>626
                     /locus_tag="ACUQR0_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HHV9111004.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          196 a          129 c          147 g          154 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcac
//
LOCUS       DBKELA010000276          601 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.282, whole genome shotgun sequence.
ACCESSION   DBKELA010000276 DBKELA010000000
VERSION     DBKELA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.282"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>601
                     /locus_tag="ACUQR0_004232"
     CDS             <1..>601
                     /locus_tag="ACUQR0_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHV9111005.1"
                     /translation="AYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGN
                     LAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVL
                     IPVVMTAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMV
                     KNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAA"
BASE COUNT          122 a          134 c          151 g          194 t
ORIGIN      
        1 ggcgtatgta tatgcccgac tggcaacaaa aaacccgcaa caaggtggcc caattgctta
       61 tgccggagaa atttcccctg catttggttt tcagacaggt gttctttatt accatgctaa
      121 ctggattggt aacctggcga ttggtattac cgctgtatct tatctttcca ccttcttccc
      181 agtattaaat gatcctgttc cggcgggtat cgcctgtatt gctatcgtct gggtatttac
      241 ctttgtaaat atgctcggcg gtacctgggt aagccgttta accactattg gtctggtgct
      301 ggttcttatt cctgtggtga tgactgctat tgttggctgg cattggtttg atgcggcaac
      361 ttatgcagct aactggaata ctgcggatac cactgatggt catgcgatca ttaaaagtat
      421 tctgctctgc ctgtgggcct tcgtgggtgt tgaatccgca gcagtaagta ctggtatggt
      481 taaaaacccg aaacgtaccg ttccgctggc aaccatgctg ggtactggtt tagcaggtat
      541 tgtttatatc gctgcgactc aggtgctttc cggtatgtat ccgtcttctg taatggcggc
      601 t
//
LOCUS       DBKELA010000277          584 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.283, whole genome shotgun sequence.
ACCESSION   DBKELA010000277 DBKELA010000000
VERSION     DBKELA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.283"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(7..584)
                     /locus_tag="ACUQR0_004233"
                     /pseudo
     CDS             complement(7..584)
                     /locus_tag="ACUQR0_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
BASE COUNT          131 a          147 c          151 g          155 t
ORIGIN      
        1 gcagggttaa ttgatggttg gcaaggaagg cgtaaagatt actgacaatt tgcccatgta
       61 gtacccggcc tgtttatcca tcacgctgaa tttatcgact gccagagtca agggtgtcgg
      121 cacacacatc acggcatagc gaataccggg cagtaccacc ggacgcacac gtttcaggtt
      181 aatgctgttc cagaccatat tttcattatt gcgcacatca atttgcccac cggtttccgg
      241 gttgtacatt ttgacccggt aatcaatgcg ttctccctcg tcggtggtgg tccctccctg
      301 acgatagatt agagaagtaa ccgtctctgc gccctgcagc tggtcgccct tcatctttga
      361 gcatgatttc ataactgatg gcattaccgt taaaaccatc gtacaggcac atgtccaaca
      421 tggtcagatc ttgcgcgtag ttgctgccgt taacgcttcc tgttggatgc aagtctaact
      481 gcacccttgg cgtggcgtgg gcgaactccg ggaagaacac gtcaatatgg ttggggtcga
      541 gcgtattcag ggtaatattt gcgaaatagt cctgggccgg gctg
//
LOCUS       DBKELA010000278          575 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.284, whole genome shotgun sequence.
ACCESSION   DBKELA010000278 DBKELA010000000
VERSION     DBKELA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.284"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="ACUQR0_004234"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="ACUQR0_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HHV9111006.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
BASE COUNT          159 a          129 c          106 g          181 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tcttt
//
LOCUS       DBKELA010000279          568 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.285, whole genome shotgun sequence.
ACCESSION   DBKELA010000279 DBKELA010000000
VERSION     DBKELA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.285"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..66)
                     /locus_tag="ACUQR0_004235"
     CDS             complement(<1..66)
                     /locus_tag="ACUQR0_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9111007.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCI"
     gene            281..>568
                     /locus_tag="ACUQR0_004236"
     CDS             281..>568
                     /locus_tag="ACUQR0_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HHV9111008.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKK"
BASE COUNT          164 a          123 c          131 g          150 t
ORIGIN      
        1 aatgcagacc gaaagtaacg tcggccaggg gaagcggcga ggtaaatcaa gggttattga
       61 agtcataatc atttcagtag aaaaactagg cctcgatttt aaatgcaaat agcaatcata
      121 ttcaataagg caagacgatc aggatcagtg aaaatgtcat ttttggcgta attaccctta
      181 ctttatgcat gaccttaaca aagacatcat ctttccgttg cagatcttcg ccctttcaaa
      241 taacgtactt tacaactttc ccgaacaagg agttgtgccc gtgttgtatg ttatttacgc
      301 tcaagacaaa gctgatagcc tcgaaaaacg cctttccgtt cgtccggcac atttagcacg
      361 tttacagtta ctgcatgatg aaggtcgttt gttgacagct ggtccaatgc cagcggtaga
      421 cagtaacgat ccaggggctg cgggatttac tggctcaacc gttattgctg aatttgaatc
      481 gctggaagca gctcaggcct gggccgatgc tgacccgtac gtagcggcag gagtctatga
      541 acacgtttca gtgaaaccat ttaagaaa
//
LOCUS       DBKELA010000280          537 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.286, whole genome shotgun sequence.
ACCESSION   DBKELA010000280 DBKELA010000000
VERSION     DBKELA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.286"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..287
                     /gene="pliG"
                     /locus_tag="ACUQR0_004237"
     CDS             <1..287
                     /gene="pliG"
                     /locus_tag="ACUQR0_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HHV9111009.1"
                     /translation="YSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDS
                     VDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(395..>537)
                     /locus_tag="ACUQR0_004238"
     CDS             complement(395..>537)
                     /locus_tag="ACUQR0_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HHV9111010.1"
                     /translation="EFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTTTKMLFMC
                     RE"
BASE COUNT          179 a          126 c           95 g          137 t
ORIGIN      
        1 agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat gccaaaaaag
       61 gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc ttcgggccag
      121 gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt cacggacaat
      181 actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga aacgacgccc
      241 gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg tcaaccgacg
      301 gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca agaggttaat
      361 gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata aataacatct
      421 ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa taacgatcat
      481 cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta aattcgc
//
LOCUS       DBKELA010000281          523 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.287, whole genome shotgun sequence.
ACCESSION   DBKELA010000281 DBKELA010000000
VERSION     DBKELA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.287"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..134)
                     /locus_tag="ACUQR0_004239"
     CDS             complement(<1..134)
                     /locus_tag="ACUQR0_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9111011.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     S"
BASE COUNT          207 a           90 c           80 g          146 t
ORIGIN      
        1 gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca aagtccgctt
       61 tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa aacatctgca
      121 gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa acctaatttt
      181 acgcctacta ccatacaaga tagaaaaagc gattattcaa ccgtgatcat attaaaaaca
      241 aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt aaccattatt
      301 gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga aaatgagcac
      361 agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat gtatttaaaa
      421 atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa ggaatgtgac
      481 aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DBKELA010000282          519 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.288, whole genome shotgun sequence.
ACCESSION   DBKELA010000282 DBKELA010000000
VERSION     DBKELA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.288"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>519
                     /locus_tag="ACUQR0_004240"
     CDS             <1..>519
                     /locus_tag="ACUQR0_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HHV9111012.1"
                     /translation="AGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTDLF
                     MTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFS
                     FAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVN
                     KTQNFEAVLQYQ"
BASE COUNT          140 a           96 c          130 g          153 t
ORIGIN      
        1 tgctggtttg agttacaaag attttggttc attcgactac agtcgtaatg tcggtgtcgc
       61 gtatgatgca gaagcattta ctgatatgtt tgttgagtgg ggcggtgact catgggctgg
      121 tactgacctg ttcatgacca accgtaccaa cggtgttgca acctatcgta acacagattt
      181 ctttggcatg gttgaaggtc taaattttgc tctgcaatat cagggtaaaa atgaaggtac
      241 aggcaactac aaagcaaatg gcgatggcca tggtttatct gcaacttaca ctattgacgg
      301 tttctcattt gctggtgctt atgcaaactc tgaccgtact gattggcagt ctggtgatgg
      361 taaaggcgaa agagccgaag tttgggcgtt gtcaacaaaa tatgacgcta acaatgttta
      421 tgctgctgtg atgtacggtg aatctcacaa catgaattcc gacgacggtg atgttgtgaa
      481 caaaacacaa aacttcgaag ctgttctgca atatcagtt
//
LOCUS       DBKELA010000283          514 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.289, whole genome shotgun sequence.
ACCESSION   DBKELA010000283 DBKELA010000000
VERSION     DBKELA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.289"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>514
                     /locus_tag="ACUQR0_004241"
     CDS             <1..>514
                     /locus_tag="ACUQR0_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HHV9111013.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KRALFLSAFAA"
BASE COUNT           97 a          173 c          140 g          104 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgggcg ttgttcctgt cggccttcgc cgcg
//
LOCUS       DBKELA010000284          507 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.290, whole genome shotgun sequence.
ACCESSION   DBKELA010000284 DBKELA010000000
VERSION     DBKELA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.290"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            279..>507
                     /locus_tag="ACUQR0_004242"
     CDS             279..>507
                     /locus_tag="ACUQR0_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHV9111014.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGG"
BASE COUNT          134 a          109 c           89 g          175 t
ORIGIN      
        1 ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt cccatcatag aacttattat
       61 ttttacgtta tttgttttcc cacttacgat aattctcttt cgtgctctga gtcacggcaa
      121 tagtattgtt ttcatcaatt tttgataatt atcacaatta agatcacaga aaagacatta
      181 cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc tgacaattcc aacatcaatg
      241 cactgataaa agatcagaat ggtctaaggc aggtctgaat gaatacccag tataattcca
      301 gttatatatt ttcgattacc ttagtcgcta cattaggtgg tttattattt ggctacgaca
      361 ccgccgttat ttccggtact gttgagtcac tcaataccgt ctttgttgct ccacaaaact
      421 taagtgaatc cgctgccaac tccctgttag gattttgcgt agccagcgct ctgattggtt
      481 gcatcatcgg cggtgccctc ggtggtt
//
LOCUS       DBKELA010000285          497 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.291, whole genome shotgun sequence.
ACCESSION   DBKELA010000285 DBKELA010000000
VERSION     DBKELA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.291"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..421
                     /locus_tag="ACUQR0_004243"
     CDS             <1..421
                     /locus_tag="ACUQR0_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9111015.1"
                     /translation="NFASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITTNR
                     RITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKL
                     KACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
BASE COUNT          133 a          115 c          134 g          115 t
ORIGIN      
        1 gaatttcgcc agcgacacaa taaaaatagc ttacaccgga gatggtcgcg tggtgtgggt
       61 cggtaaggat gtgacaggca tcgagccacg caatgccagc gttatcgaag ttccggatat
      121 taccactaac cgacggatta ccgcgccggg ttactggttt taccgcaatg atgaatttgt
      181 ctttgactac agactcaaag cggaagatga gcgtgatgcc cttctggctc aggtcagtgc
      241 ccggacaggg gaatgggaag aagacctgct gctggggtta atcagcgacg aagataaaga
      301 aaagctgaaa gcctgtcgta tttacgcgaa atcgctgcag gcgatggatt tcagcaccat
      361 cactgataaa tcctcataca acgccattga atggcccgcc tctccggaag gttcttcctg
      421 atttaattta tcgcgagaaa aacaatgtct gtagtgatat caggtgcgct gactgatggc
      481 gcaggtatcc ccatgtc
//
LOCUS       DBKELA010000286          495 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.292, whole genome shotgun sequence.
ACCESSION   DBKELA010000286 DBKELA010000000
VERSION     DBKELA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.292"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>495
                     /locus_tag="ACUQR0_004244"
     rRNA            <1..>495
                     /locus_tag="ACUQR0_004244"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          128 a          120 c          144 g          103 t
ORIGIN      
        1 aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat
       61 gcaacgcgaa gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt
      121 gccttcggga accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt
      181 tgggttaagt cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga
      241 actcaaagga gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat
      301 ggcccttacg accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc
      361 gcgagagcaa gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac
      421 tccatgaagt cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg
      481 ggccttgtac acacc
//
LOCUS       DBKELA010000287          495 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.293, whole genome shotgun sequence.
ACCESSION   DBKELA010000287 DBKELA010000000
VERSION     DBKELA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.293"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..>495)
                     /locus_tag="ACUQR0_004245"
     rRNA            complement(<1..>495)
                     /locus_tag="ACUQR0_004245"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DBKELA010000288          490 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.294, whole genome shotgun sequence.
ACCESSION   DBKELA010000288 DBKELA010000000
VERSION     DBKELA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.294"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..142
                     /locus_tag="ACUQR0_004246"
     CDS             <1..142
                     /locus_tag="ACUQR0_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HHV9111016.1"
                     /translation="ISDEDREKLKAYRIYAKSLQAMDFSVITDKTSYNAIEWPASPEI
                     SS"
     gene            166..>490
                     /locus_tag="ACUQR0_004247"
     CDS             166..>490
                     /locus_tag="ACUQR0_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HHV9111017.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEE"
BASE COUNT          139 a          107 c          124 g          120 t
ORIGIN      
        1 aatcagcgac gaagaccggg aaaaactgaa agcgtaccgc atttacgcga aatcgctgca
       61 ggcgatggat ttcagtgtca tcactgataa aacctcatac aacgccattg aatggcccgc
      121 ttctccggaa atttcttcct gatttaattt atcgcgagaa aaactatgtc tgtagtgata
      181 tcaggtgcgc tgactgatgg cgcaggtatc tccatgtccg gataccatat tattctgaaa
      241 tcccgggtaa acaccccgga agtggtgatg cacactgttg ctgatgtgat gacaggaaac
      301 gatggtgaat actgtttcca tgcgcggact ggaaaatatg gtgtgtatct gaaacaggac
      361 tggcacaacg agtacaacgt tggcgacatt gctgtatatg aggactcaaa gcccggtacg
      421 ctgaatgact ttctgattgc tcctgatgag ggcgacctga aaccagatgt cgtcaaacgc
      481 tttgaggaaa
//
LOCUS       DBKELA010000289          489 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.295, whole genome shotgun sequence.
ACCESSION   DBKELA010000289 DBKELA010000000
VERSION     DBKELA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.295"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..124)
                     /gene="dapF"
                     /locus_tag="ACUQR0_004248"
     CDS             complement(<1..124)
                     /gene="dapF"
                     /locus_tag="ACUQR0_004248"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HHV9111018.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVG"
     gene            complement(161..364)
                     /gene="lptM"
                     /locus_tag="ACUQR0_004249"
                     /gene_synonym="yifL"
     CDS             complement(161..364)
                     /gene="lptM"
                     /locus_tag="ACUQR0_004249"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HHV9111019.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT          121 a          118 c          125 g          125 t
ORIGIN      
        1 accctacccc caggtgccga tccgccaggc gacgaatcag ctccggtgaa aaaaagacat
       61 tctgcgttac cgcgtcgacg accataaaat cgttgccaag gccatgcatt ttcgagaact
      121 gcatcattta ctccaatcac gcgggtacag aaactgactt ttagtaattc acctgggatg
      181 gaccatcgcc agtggcgcga tcgtttttat ccggcaccgt ggattgcgtt tgcgtctcta
      241 ccggtttggt cggcggcggt gcgtttttat ctgcaggcgg gaaatagagc ggacctttca
      301 gaccgcagcc cgtcaggctg aagagagtaa gtaatacagt gagtgcctta aacacgtttt
      361 tcattatgga ttgcctgtaa agttcatcgc tttctgcttt ctatcatcgc aggagaaggc
      421 gtaaaagcaa gcgtttagcg actctttatg aagtctgaaa agtgaggaaa tgctgcgagt
      481 ataacgctt
//
LOCUS       DBKELA010000290          474 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.296, whole genome shotgun sequence.
ACCESSION   DBKELA010000290 DBKELA010000000
VERSION     DBKELA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.296"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..451)
                     /locus_tag="ACUQR0_004250"
     CDS             complement(<1..451)
                     /locus_tag="ACUQR0_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HHV9111020.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSEC"
BASE COUNT          126 a          120 c          101 g          127 t
ORIGIN      
        1 agcattccga accctcttta cggatcgttg gctccagaat tttccactgg tattccgtca
       61 gtgcgtgggc ttcttccagc cacaacatat cgatcccctc cagtgactta atttcttcga
      121 tgttgcgcca taatccataa aaaacaaatt ccgagccagt aacccggtta atgattttgt
      181 tgttcagaat acggaaacgg tgccgcagac caaacctgtc tatctgaatt ttgagcaggg
      241 tatacaccga ctcttcgatt ttattctgga tctgacgggc acaacaaaaa cgcagggtgt
      301 atttattcga cagaaatatg gcaatgccag cggcatccca tgattttgac gatgaccgac
      361 caccataaag cactttgtta cgtgcccgca tagtccagaa actacgtaaa gccggattca
      421 gcgtcggttt ggatgtcaga gtagaagtca ttgaggtcac gctctccatt gcca
//
LOCUS       DBKELA010000291          457 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.297, whole genome shotgun sequence.
ACCESSION   DBKELA010000291 DBKELA010000000
VERSION     DBKELA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.297"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>457
                     /locus_tag="ACUQR0_004251"
     CDS             <1..>457
                     /locus_tag="ACUQR0_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHV9111021.1"
                     /translation="QHFAGMQLRDITESKIYSAMQKMTNRRHEENWKLRAEACRKKGK
                     PVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPH
                     EAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINP"
BASE COUNT          160 a          101 c          108 g           88 t
ORIGIN      
        1 ttcaacattt cgcagggatg caactaagag acatcactga atcaaaaatt tattcagcaa
       61 tgcagaaaat gacgaaccgg cgtcatgagg aaaactggaa actcagggca gaagcatgca
      121 gaaaaaaagg gaaacctgtt ccagaataca cgccaaaacc agcgtccgtt gcaacgaagg
      181 ctacgcatct ttcatttata aaggccctac taagagccgc agagcgtgaa tggaaaatgc
      241 tggataaggc accaattatt aaagtgcctc aaccaaagaa taaacggatc cgctggctgg
      301 agccccatga agcacaaagg ctgattgatg aatgtccgga gccattaaag tctgttgttg
      361 aatttgcact ggcaacaggc ttaagacgct cgaacatcat caaccttgaa tggcaacaaa
      421 tagatatgca gcgccgggtg gcatggataa acccgga
//
LOCUS       DBKELA010000292          431 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.298, whole genome shotgun sequence.
ACCESSION   DBKELA010000292 DBKELA010000000
VERSION     DBKELA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.298"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..431
                     /locus_tag="ACUQR0_004252"
                     /pseudo
     CDS             1..431
                     /locus_tag="ACUQR0_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          103 a          121 c          127 g           80 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt c
//
LOCUS       DBKELA010000293          417 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.299, whole genome shotgun sequence.
ACCESSION   DBKELA010000293 DBKELA010000000
VERSION     DBKELA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.299"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
BASE COUNT          118 a          111 c           82 g          106 t
ORIGIN      
        1 atgcggtgcc tggtgcctcc aggtgacgtt aaccagttaa caattacagt tggctttccc
       61 acccaaacca ataaggacta acatgacttt taactgtgcc gcgtgcgcat agccgcattc
      121 accgcatcac aaaattcact ttaaaaaggg cggacatcag ccagcaatta aaccgatgcc
      181 gccaactggt acttcacaca gcaatgtcgt tatttacaac cggaagcgca ctcccaccat
      241 ttaaatttca cagacaagac cgactcttta tggataccgg aaatgcgcct tcgtgttgtg
      301 cccggtttta tttcaccact tccgggcttt ggtggtatct ttactgaagt tctcacacaa
      361 ccagtaagga aatgaatatg ccaacgtatc tcgccagagt agaactatat aatgctg
//
LOCUS       DBKELA010000294          412 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.300, whole genome shotgun sequence.
ACCESSION   DBKELA010000294 DBKELA010000000
VERSION     DBKELA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.300"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            1..412
                     /locus_tag="ACUQR0_004253"
                     /pseudo
     CDS             1..412
                     /locus_tag="ACUQR0_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          105 a          106 c          120 g           81 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cg
//
LOCUS       DBKELA010000295          400 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.301, whole genome shotgun sequence.
ACCESSION   DBKELA010000295 DBKELA010000000
VERSION     DBKELA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.301"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..131
                     /locus_tag="ACUQR0_004254"
     CDS             <1..131
                     /locus_tag="ACUQR0_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHV9111022.1"
                     /translation="YYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          116 a          104 c           82 g           98 t
ORIGIN      
        1 cttactacac acgcaaaatg agtcagggaa aacgacacaa tcaggcgctt atcgccctgg
       61 cgagacgacg ctgcgacgtt ctgttcgcca tgatgcgcga cgggactttt tataccccgc
      121 aggggtcata acatgcttga caacttaata ggggcccccc ccgacagacc tgtgctatcg
      181 gaaagaaaaa aggccatcag caaaactctg atgacctgaa tcacctttac caatattgta
      241 tgaaaaaaca cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta
      301 gcattcttaa ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat
      361 ccccaatgtt tttgctggcg ggtccttttt tctacggtcc
//
LOCUS       DBKELA010000296          397 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.302, whole genome shotgun sequence.
ACCESSION   DBKELA010000296 DBKELA010000000
VERSION     DBKELA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.302"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(27..>397)
                     /locus_tag="ACUQR0_004255"
     CDS             complement(27..>397)
                     /locus_tag="ACUQR0_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9111023.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGTWYNVASI"
BASE COUNT          101 a          101 c           84 g          111 t
ORIGIN      
        1 agtaatattt tttaaatgca acattgttaa atactcgcta cgttatacca ggtgccatta
       61 atcagttttt gcagtggtcg gtaatacaca ccgtcaacgt tatccgctga gttacggccg
      121 gtatccgata tcgccatccc tgacagcccg tgtcccgaag gtgcgcaaaa tctccacgaa
      181 acggtgttac tgccggggct gtaatacatt tcatggccat accgtacatc ctgtaccccc
      241 tctgttcgat atttatagcg ggcatcgaag tttccgtaat ttaacggaat tacctgtccg
      301 ttaacagtga acactatgct gttatccggg tttctctggc tgaaaaaatg ccagcctgaa
      361 tcatcgccaa gctctgcaac aacaggcctg gatggat
//
LOCUS       DBKELA010000297          396 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.denovo.303, whole genome shotgun sequence.
ACCESSION   DBKELA010000297 DBKELA010000000
VERSION     DBKELA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.denovo.303"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..>396
                     /locus_tag="ACUQR0_004256"
     CDS             <1..>396
                     /locus_tag="ACUQR0_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HHV9111024.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAG"
BASE COUNT           94 a           85 c          115 g          102 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaat
//
LOCUS       DBKELA010000298         5818 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.guided.169, whole genome shotgun sequence.
ACCESSION   DBKELA010000298 DBKELA010000000
VERSION     DBKELA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.guided.169"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="ACUQR0_004257"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="ACUQR0_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HHV9111025.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2123
                     /locus_tag="ACUQR0_004258"
                     /pseudo
     CDS             1596..2123
                     /locus_tag="ACUQR0_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2144..2617
                     /gene="dfrA14"
                     /locus_tag="ACUQR0_004259"
     CDS             2144..2617
                     /gene="dfrA14"
                     /locus_tag="ACUQR0_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HHV9111026.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2692..2967
                     /locus_tag="ACUQR0_004260"
                     /pseudo
     CDS             2692..2967
                     /locus_tag="ACUQR0_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2967..3803
                     /locus_tag="ACUQR0_004261"
     CDS             2967..3803
                     /locus_tag="ACUQR0_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HHV9111027.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3910..4314)
                     /locus_tag="ACUQR0_004262"
                     /pseudo
     CDS             complement(3910..4314)
                     /locus_tag="ACUQR0_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061586541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4358..4747)
                     /locus_tag="ACUQR0_004263"
     CDS             complement(4358..4747)
                     /locus_tag="ACUQR0_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9111028.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4965..5405
                     /locus_tag="ACUQR0_004264"
     CDS             4965..5405
                     /locus_tag="ACUQR0_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HHV9111029.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5464..>5818)
                     /locus_tag="ACUQR0_004265"
     CDS             complement(5464..>5818)
                     /locus_tag="ACUQR0_004265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHV9111030.1"
                     /translation="TSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAP
                     ATLHGHAINCVPVHRFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVF
                     GWFVACFTGFPSETP"
BASE COUNT         1397 a         1539 c         1519 g         1363 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgttaacc caggatgaga accttgaaag tatcattgat
     2161 agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct ggtccgcgaa
     2221 aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg tgggtcgcaa
     2281 gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta cccgctcagg
     2341 ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg ccatggacag
     2401 gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt accgagaaac
     2461 attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag agggggatgt
     2521 tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact ttacttcaaa
     2581 cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa tcgacggcaa
     2641 aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga cgatatggtt
     2701 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2761 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2821 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2881 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2941 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     3001 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     3061 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     3121 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     3181 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     3241 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     3301 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     3361 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     3421 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     3481 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     3541 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     3601 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     3661 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3721 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3781 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3841 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3901 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4441 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4501 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4561 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     4621 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     4681 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     4741 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     4801 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     4861 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     4921 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     4981 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5041 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5101 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5161 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5221 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5281 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5341 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5401 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5461 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5521 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     5581 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     5641 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     5701 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     5761 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgta
//
LOCUS       DBKELA010000299         5663 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.guided.174, whole genome shotgun sequence.
ACCESSION   DBKELA010000299 DBKELA010000000
VERSION     DBKELA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.guided.174"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            21..284
                     /locus_tag="ACUQR0_004266"
     CDS             21..284
                     /locus_tag="ACUQR0_004266"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HHV9111031.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            277..663
                     /locus_tag="ACUQR0_004267"
     CDS             277..663
                     /locus_tag="ACUQR0_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HHV9111032.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            671..1357
                     /locus_tag="ACUQR0_004268"
     CDS             671..1357
                     /locus_tag="ACUQR0_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHV9111033.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="ACUQR0_004269"
     CDS             complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="ACUQR0_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HHV9111034.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2040..3245
                     /gene="tet(B)"
                     /locus_tag="ACUQR0_004270"
     CDS             2040..3245
                     /gene="tet(B)"
                     /locus_tag="ACUQR0_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HHV9111035.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="ACUQR0_004271"
     CDS             complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="ACUQR0_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HHV9111036.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4039..4434
                     /locus_tag="ACUQR0_004272"
                     /pseudo
     CDS             4039..4434
                     /locus_tag="ACUQR0_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4465..>5663)
                     /locus_tag="ACUQR0_004273"
     CDS             complement(4465..>5663)
                     /locus_tag="ACUQR0_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HHV9111037.1"
                     /translation="HKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRNL
                     PTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKR
                     LMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGF
                     KVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQ
                     LVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVH
                     AQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHG
                     YALGKL"
BASE COUNT         1568 a         1044 c         1230 g         1821 t
ORIGIN      
        1 ctgaacataa aacactatca ataagttgga gtcattaccg accactatta agtcatgtag
       61 ctggttttat ttcaattgaa cgttttcaaa gtctagctac agaaggaaaa atgttatcgc
      121 tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa aaatcatgtt ttacatgcga
      181 aagctcaaca agaagggcga gagtcaatat ttgattttta caaaattagt attgctcata
      241 ttactcgcga atattcattt aaaaaggaca aggataatgt ttgatgttca cgttgtttta
      301 gataatcaaa taggacaatt agcattacta ggaaaaacat taggtaataa aggtattgga
      361 ttggaagggg gagggatatt tacggttggt gatgaatgcc atgctcattt tcttgttgaa
      421 caaggaaagg aagctaaaat agcgctagag caagctggac tgttagtact tgcgatccgg
      481 acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg aacttggcga aatagcacga
      541 gtattggcgg agaataacat taatatttta gtgcaataca gtgaccatgc taaccaactg
      601 atattaataa cggacaatga tagtatggct gcatctgtta cgctcccttg ggcaataaag
      661 tgaacttgcg atggctaatt taatacgaaa agaggttacc tttgagtcct caatagccgc
      721 gataggggcg gctatgtctg acatttcacg agttaaaata ctcagtgctt tgatggatgg
      781 gcgagcttgg acggccactg agctaagttc tgtggcgaat atatcagctt caacggcgag
      841 cagtcattta tctaaattat tagattgcca gctaatcaca gtagtagctc aaggcaagca
      901 tcgttatttt cggctagcag gaaaagatat tgctgaattg atggaaagta tgatggggat
      961 ctccttaaac catggcgtac atgccaaagt ttccacgcca gtgcatttac gaaaagcacg
     1021 tacttgctat gatcatttag ctggcgaagt tgccgttaag atctatgatt ccctttgtca
     1081 acagcaatgg atcactgaaa atggttcaat gatcacatta agtggtattc aatattttca
     1141 tgaaatggga attgacgttc cttccaaaca ttcacgtaaa atctgttgtg cgtgtttaga
     1201 ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga gccgcattat tttcgcttta
     1261 tgaatctaaa gggtggttaa ctcgacatct tggttaccgt gaagttacca tcacggaaaa
     1321 aggttatgct gcttttaaga cccactttca catttaagtt gtttttctaa tccgcatatg
     1381 atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata
     1441 gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg
     1501 acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt
     1561 gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg
     1621 agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga
     1681 tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt
     1741 tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg
     1801 tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc
     1861 ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat
     1921 gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta atttttgttg
     1981 acactctatc attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa
     2041 tgaatagttc gacaaagatc gcattggtaa ttacgttact cgatgccatg gggattggcc
     2101 ttatcatgcc agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta
     2161 accactttgg cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc
     2221 ttggaaaaat gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg
     2281 catcgctgga ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc
     2341 gtttgctttc agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata
     2401 ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg
     2461 gtttaatagc ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct
     2521 tttttatcgc tgcgttgcta aatattgtcg ctttccttgt ggttatgttt tggttccgtg
     2581 aaaccaaaaa tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcgaatt
     2641 cggtatacat cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc
     2701 aattgatagg ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat
     2761 ggaatagcat gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc
     2821 aagcctttgt ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg
     2881 gatttattgc agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag
     2941 ttttccctgt tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag
     3001 tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc
     3061 ttaccaatgc aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac
     3121 taccaatttg ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc
     3181 tgctatcgat gaccttcatg ttaacccctc aagctcaggg gagtaaacag gagacaagtg
     3241 cttagttatt tcgtcaccaa atgatgttat tccgcgaaat ataatgaccc tcttgataac
     3301 ccaagagggc attttttacg ataaagaaga tttagcttca aataaaacct atctatttta
     3361 tttatctttc aagctcaata aaaagccgcg gtaaatagca ataaattggc cttttttatc
     3421 ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc ataaaccagc cattgagtaa
     3481 gtttttaagc acatcatcat cataagcttt aagttggttc tcttggatca atttgctgac
     3541 aatggcgttt accttaccag taatgtattc aaggctaatt ttttcaagtt cattccaacc
     3601 aatgataggc atcacttctt ggatagggat aaggttttta ttattatcaa taatataatc
     3661 aagataatgt tcaaatatac tttctaaggc agaccaacca tttgttaaat cagtttttgt
     3721 tgtgatgtag gcatcaatca taattaattg ctgcttataa caggcactga gtaattgttt
     3781 tttattttta aagtgatgat aaaaggcacc tttggtcacc aacgcttttc ccgagatctc
     3841 atctattgaa acagcttgat agcctttttc aacaaacaat attcgtgctg agttaaccag
     3901 tgattgatag gtactcttaa aattttcttg ttgatgattt ttattttcca tgatagattt
     3961 aaaataacat accgtcagta tgtttatggt atcatgatga tgtggtcgtg acaatcttaa
     4021 gaacatttag gttattttat gtatattgaa cagcattctc gctatcaaaa taaagctaat
     4081 aacatccaat taagatatga tgataagcag tttcatacaa cggttatcaa agatgttcta
     4141 ttatggattg aacataattt agatcagtct ttactgcttg atgatgtggc gaataaagcg
     4201 ggttatacca agtggtattt tcagcggctg ttcaaaaaag taacaggggt cacactggct
     4261 agctatattc gtgctcgtcg tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa
     4321 actatccttg agatcgcatt aaaatatcaa tttgattccc aacaatcttt tacacgtcga
     4381 tttaagtaca tttttaaggt tacaccaagt tattatcggc gtaataaatt atgggaattg
     4441 gaggcaatgc actgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa
     4501 attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga
     4561 atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt
     4621 gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca
     4681 tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt
     4741 tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc
     4801 aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg
     4861 taagttagtt gctagaaccc atggctcctt tgccgacgct gagtagattt taggtgacgg
     4921 gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata
     4981 caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt
     5041 tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag
     5101 gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac
     5161 ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact
     5221 cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc
     5281 tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc
     5341 tcgcaatacc ataagtcgtt tttgctcacg aatatcagac cagtcaacaa gtacaatggg
     5401 catcgtattg cccgaacaga taaagctagc atgccaacgg tatacagcga gtcgctcttt
     5461 gtggaggtga cgattaccta acaatcggtc gattcgtttg atgttatgtt ttgttctcgc
     5521 tttggttggc aggttacggc caagttcggt aagagtgaga gttttacagt caagtaatgc
     5581 gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt aattcggggt aatgactcca
     5641 acttactgat agtgttttat gtt
//
LOCUS       DBKELA010000300         4736 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.guided.194, whole genome shotgun sequence.
ACCESSION   DBKELA010000300 DBKELA010000000
VERSION     DBKELA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.guided.194"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..975)
                     /gene="pepD"
                     /locus_tag="ACUQR0_004274"
     CDS             complement(<1..975)
                     /gene="pepD"
                     /locus_tag="ACUQR0_004274"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HHV9111038.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLN"
     gene            1237..1695
                     /gene="gpt"
                     /locus_tag="ACUQR0_004275"
     CDS             1237..1695
                     /gene="gpt"
                     /locus_tag="ACUQR0_004275"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HHV9111039.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1787..3031
                     /gene="frsA"
                     /locus_tag="ACUQR0_004276"
     CDS             1787..3031
                     /gene="frsA"
                     /locus_tag="ACUQR0_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HHV9111040.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3089..3490
                     /gene="crl"
                     /locus_tag="ACUQR0_004277"
     CDS             3089..3490
                     /gene="crl"
                     /locus_tag="ACUQR0_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HHV9111041.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(3529..4584)
                     /gene="phoE"
                     /locus_tag="ACUQR0_004278"
     CDS             complement(3529..4584)
                     /gene="phoE"
                     /locus_tag="ACUQR0_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HHV9111042.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1168 a         1163 c         1163 g         1242 t
ORIGIN      
        1 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc
       61 tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat
      121 ctcctgatag gtattcacca gagatttcag tgcgtcgact ttatcagctg cgacagcaat
      181 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag
      241 gcgcaggtcc agttcttctg catgacccgc caggaagcgc accagcagtt tgttggcatt
      301 acccaggcca acgtggattt caccgccgga gtgaccgcct ttcagacctt ttaaggttaa
      361 cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa
      421 gtcgataccc cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
      481 cagaatatca gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc
      541 ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac
      601 cgccagtgca gaggccatac caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt
      661 aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc
      721 gttatttttc tgcggcacca tatcgaggtg ggcctgtaag acgaccggtt tacgattttc
      781 catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg
      841 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
      901 cgggtgagga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
      961 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     1021 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt attgagaata
     1081 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     1141 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     1201 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     1261 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     1321 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggggcgtt actggcgcgt
     1381 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     1441 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     1501 gacctggtgg ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac
     1561 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     1621 atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     1681 atctccggtc gctaatcttt tcaacgcctg gcattgccgg gcgttgttct ttttaacttc
     1741 aggcaggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     1801 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     1861 ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc
     1921 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga
     1981 aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt
     2041 atacgatacc gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc
     2101 gatggtgtgg caacaaaaag cctgtacgga agaagatccg aaactcagtg gtcgacactg
     2161 gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct
     2221 ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc
     2281 gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt
     2341 gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc
     2401 gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgcgat
     2461 gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca cccaggactc
     2521 cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac
     2581 tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatacct
     2641 tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag
     2701 tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt
     2761 ggggatgcat gatgcttccg acgaagcgtt acgcgtggag ctgaatcgct attcattaaa
     2821 agtgcaagga ttgctggggc gtcgctgccc aacgccaatg ttatcaggct actggaagaa
     2881 cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa
     2941 attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat
     3001 caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc aatttggtaa
     3061 aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc cgaagagcag
     3121 attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa
     3181 tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg
     3241 tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta cctacagcta
     3301 ccagtttggt ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagatactga
     3361 agtggttgaa cgactggagc acaccctgcg tgagtttcac gagaagctgc gtgaactgct
     3421 gacgacgctg aatctgaagc tggaaccggc gaatgatttt cgtgatgaac cggtgaagtt
     3481 aacggcgtga gtgaaatgtg ccggatgcat cacatccggc aatattcatt aaaactgata
     3541 cgtcatccca accgcgacag tatcatcatt attaatattc agcttattat cgctatccag
     3601 ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat acgtagcacc
     3661 gacgtcgata taattgacca gatcttcatc accgatgccc tcaatatctt tccctttcga
     3721 taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc
     3781 aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc gtgtttcaga
     3841 atagaaagtt gccagataaa tattattggc atcgtatttc agacctgttg cccatgcttc
     3901 tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt
     3961 ataggcccca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa
     4021 gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt
     4081 taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct
     4141 ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa tcgccaccaa attccgggaa
     4201 catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga
     4261 acccaaatct ttatatttca acccggcaaa agcgagacgc gttttttgct gtgcagtatc
     4321 actctccgct ttatttccgg caaactccgc ttcccaccga ccataaccag tcagttgatc
     4381 gttaatttga gtttcacctt taaaaccaaa acggatataa ctctggtcgc catctttact
     4441 ggcgttatca ctcatataat gcatggcttt aactttgcca tagacatcca gtttattacc
     4501 gtctttatta tatatttctg cagcctgtac agatgcagat gccacaatgc ccatcaccac
     4561 taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg cgaatattca
     4621 gcgggagagc cccgttgaaa acaggaaagt ttttaacctg agattgttaa agatatatta
     4681 cagattaatg atattctgaa aatgggtaat gctgccaact tactgattta gtgtat
//
LOCUS       DBKELA010000301         4666 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.guided.195, whole genome shotgun sequence.
ACCESSION   DBKELA010000301 DBKELA010000000
VERSION     DBKELA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.guided.195"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            complement(<1..348)
                     /locus_tag="ACUQR0_004279"
     CDS             complement(<1..348)
                     /locus_tag="ACUQR0_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HHV9111043.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPF"
     gene            complement(351..911)
                     /locus_tag="ACUQR0_004280"
     CDS             complement(351..911)
                     /locus_tag="ACUQR0_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHV9111044.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(1037..1237)
                     /locus_tag="ACUQR0_004281"
     CDS             complement(1037..1237)
                     /locus_tag="ACUQR0_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HHV9111045.1"
                     /translation="MNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQ
                     ARASTATETSVKPDACDSPPSG"
     gene            complement(1337..1621)
                     /locus_tag="ACUQR0_004282"
                     /pseudo
     CDS             complement(1337..1621)
                     /locus_tag="ACUQR0_004282"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1590..2603)
                     /gene="intI1"
                     /locus_tag="ACUQR0_004283"
     CDS             complement(1590..2603)
                     /gene="intI1"
                     /locus_tag="ACUQR0_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HHV9111046.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2813..3643
                     /locus_tag="ACUQR0_004284"
     CDS             2813..3643
                     /locus_tag="ACUQR0_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HHV9111047.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            3756..4594
                     /gene="aadA1"
                     /locus_tag="ACUQR0_004285"
                     /pseudo
     CDS             3756..4594
                     /gene="aadA1"
                     /locus_tag="ACUQR0_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1166 a         1246 c         1240 g         1014 t
ORIGIN      
        1 gaagggccgg aaaccgaaca cggtttgcag ctcgctcagg tgctcgcgcc gggtctgctc
       61 ccgctggccg tactcgttcc agctttcgac gccgaccttg agctggtcgg cgaccagctt
      121 caacaagggc gggaacggta gttcatcgac gcccaggatg acgccgggaa agcgcaggta
      181 acagagctgc accgcgaagc ccagccgatt ggctggcccg cgccgctgtc ggatgatcga
      241 gaggtcggta tcgttgaatg tgtaatgtcg gatcaggtcg tccttggagt ccggcaacgc
      301 cagcaggctt tcccgctcgg cggcggacag gatggaacga cgtggcatat ttactgatcc
      361 gttctcaagt attgatacag ggtttcgcga ctgattccga attcacgagc aagcttggtc
      421 ttttgctcgc cagcctcgac acgttggcgc agttcggcaa tacgctcaga cgacagggat
      481 ttcttcctgc cacggtaagc cccgcgttgc ttggcgagcg caataccctc gcgctgacgc
      541 tcgcggatca gggcgcgctc gaactcggcg aacgcgccca tcaccgagag catcaggttc
      601 gccatcggag agtcttcgcc agtaaaactg aggtgttcct tgacgaattc gatatgcacg
      661 ccgcgttgtg tcagcgtttg cacgatccgg cgcaaatcat cgagattgcg cgccaggcga
      721 tccatgctat gcaccaccac ggtgtcgccg gtgcgggcga agcttatcag cgcttccagt
      781 tgcggacgct tgacatcctt gccggatgcc ttgtcgctaa aagcgcgatc aaccttgacg
      841 ccttccagtt gccgttccgg gttctggtcg aaggtgctga ccctgatata cccaatgcgc
      901 tgtccagtca tggaattccc tgcaaaatgt cagggaagac tctatgacct tcaacgagat
      961 atgtcaataa attcaaaatt caatcctatc ctgacgcaat ttacacatgg catctgacat
     1021 caggttaggg tatgcctcaa cctgacggcg gcgaatcaca agcgtccggt ttgacgctgg
     1081 tttcggtcgc agtgctggcc cgcgcctgga agcgctgata gcactccagc ccgcagaaat
     1141 gctccacgta ctcggcccct tccggcgtga aggcggcatc gagcgggatt tccttgcagc
     1201 acacgcagca actggtgctc ggttcattgg cgttcatggt ggtgttcctc cattggttga
     1261 cgaagccgac gaaggccgcc gccggcatcg gcctggcgaa caggaaaccc tgcaccgtgt
     1321 cgcaacccgc ctgccgcaac cacgcaaggc agtcgggtgt ttccacaccc tcggctacca
     1381 cctccattgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
     1441 cagcggaggg gttggatcca tcaggcaacg acgggctgct gccggccatc agcggacgca
     1501 gggaggactt tccgcaaccg gccgttcgat gcggcaccga tggccttcgc gcaggggtag
     1561 tgaatccgcc aggattgact tgcgctgccc tacctctcac tagtgagggg cggcagcgca
     1621 tcaagcggtg agcgcactcc ggcaccgcca actttcagca catgcgtgta aatcatcgtc
     1681 gtagagacgt cggaatggcc gagcagatcc tgcacggttc gaatgtcgta accgctgcgg
     1741 agcaaggccg tcgcgaacga gtggcggagg gtgtgcggtg tggcgggctt cgtgatgcct
     1801 gcttgttcta cggcacgttt gaaggcgcgc tgaaaggtct ggtcatacat gtgatggcga
     1861 cgcacgacac cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac ccagaaccac
     1921 ggccaggaat gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag cgcaacgccg
     1981 ctgcggccct cggcctggtc cttcagccac catgcccgtg cacgcgacag ctgctcgcgc
     2041 aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc
     2101 cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca
     2161 ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga
     2221 aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc
     2281 cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag
     2341 aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc
     2401 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg
     2461 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     2521 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     2581 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     2641 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2701 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttgggcaa acccggagcc
     2761 tcattaattg ttagccgtta aaattaagcc ctttaccaaa ccaatactta ttatgaaaaa
     2821 cacaatacat atcaacttcg ctattttttt aataattgca aatattatct acagcagcgc
     2881 cagtgcatca acagatatct ctactgttgc atctccatta tttgaaggaa ctgaaggttg
     2941 ttttttactt tacgatgcat ccacaaacgc tgaaattgct caattcaata aagcaaagtg
     3001 tgcaacgcaa atggcaccag attcaacttt caagatcgca ttatcactta tggcatttga
     3061 tgcggaaata atagatcaga aaaccatatt caaatgggat aaaaccccca aaggaatgga
     3121 gatctggaac agcaatcata caccaaagac gtggatgcaa ttttctgttg tttgggtttc
     3181 gcaagaaata acccaaaaaa ttggattaaa taaaatcaag aattatctca aagattttga
     3241 ttatggaaat caagacttct ctggagataa agaaagaaac aacggattaa cagaagcatg
     3301 gctcgaaagt agcttaaaaa tttcaccaga agaacaaatt caattcctgc gtaaaattat
     3361 taatcacaat ctcccagtta aaaactcagc catagaaaac accatagaga acatgtatct
     3421 acaagatctg gataatagta caaaactgta tgggaaaact ggtgcaggat tcacagcaaa
     3481 tagaacctta caaaacggat ggtttgaagg gtttattata agcaaatcag gacataaata
     3541 tgtttttgtg tccgcactta caggaaactt ggggtcgaat ttaacatcaa gcataaaagc
     3601 caagaaaaat gcgatcacca ttctaaacac actaaattta taaaaaatct aatggcaaaa
     3661 tcgcccaacc cttcaatcaa gtcgggacgg ccaaaagcaa gcttttggct cccctcgctg
     3721 gcgctcggcg ccccttattt caaacgttaa acatcatgag ggaagtggtg atcgccgaag
     3781 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     3841 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     3901 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     3961 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     4021 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     4081 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     4141 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     4201 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     4261 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     4321 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     4381 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     4441 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     4501 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     4561 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca
     4621 taattgcggt gatgctgcca acttactgat ttagtgtatg atggtg
//
LOCUS       DBKELA010000302         3199 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.guided.215, whole genome shotgun sequence.
ACCESSION   DBKELA010000302 DBKELA010000000
VERSION     DBKELA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.guided.215"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            <1..362
                     /locus_tag="ACUQR0_004286"
     CDS             <1..362
                     /locus_tag="ACUQR0_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HHV9111048.1"
                     /translation="QERFKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARK
                     ESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEA
                     DAADKAEAANNKEKANG"
     gene            355..1089
                     /gene="frdB"
                     /locus_tag="ACUQR0_004287"
     CDS             355..1089
                     /gene="frdB"
                     /locus_tag="ACUQR0_004287"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HHV9111049.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1100..1495
                     /gene="frdC"
                     /locus_tag="ACUQR0_004288"
     CDS             1100..1495
                     /gene="frdC"
                     /locus_tag="ACUQR0_004288"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HHV9111050.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1506..1865
                     /gene="frdD"
                     /locus_tag="ACUQR0_004289"
     CDS             1506..1865
                     /gene="frdD"
                     /locus_tag="ACUQR0_004289"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HHV9111051.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1928..3061
                     /locus_tag="ACUQR0_004290"
     CDS             1928..3061
                     /locus_tag="ACUQR0_004290"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HHV9111052.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          784 a          841 c          832 g          742 t
ORIGIN      
        1 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc
       61 tgctctacac cattgaactg agccacggtc tgaacgttgc tgaatgtatg gcgcactccg
      121 caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc
      181 gtgacgacgt caactttctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
      241 gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
      301 gcgaagcgga tgcagccgat aaggcggaag cagccaataa taaggagaag gcgaatggct
      361 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
      421 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc
      481 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
      541 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
      601 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
      661 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
      721 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
      781 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
      841 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
      901 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggtgta
      961 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     1021 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     1081 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     1141 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     1201 tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc
     1261 ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     1321 gatcactctg acggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc
     1381 ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     1441 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     1501 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     1561 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     1621 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     1681 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     1741 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtgcct gcgggcaaat
     1801 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt gtcgttacaa
     1861 tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     1921 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt actccacatt
     1981 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     2041 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     2101 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     2161 aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga
     2221 aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta aacagtggaa
     2281 tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc
     2341 ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     2401 ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     2461 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact
     2521 caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg
     2581 atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg
     2641 tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttac ccccccttga
     2701 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     2761 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     2821 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     2881 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     2941 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     3001 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     3061 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     3121 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     3181 cagctgcatt tctggtcgt
//
LOCUS       DBKELA010000303         1285 bp    DNA     linear   BCT 11-SEP-2025
DEFINITION  TPA_asm: Shigella flexneri strain EF0003065-014741
            SAMN51230420-rid27608593.guided.256, whole genome shotgun sequence.
ACCESSION   DBKELA010000303 DBKELA010000000
VERSION     DBKELA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN51230420
            Sequence Read Archive: SRR35310109
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002918914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002918914.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/11/2025 01:41:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 4,292
            CDSs (total)                      :: 4,209
            Genes (coding)                    :: 3,830
            CDSs (with protein)               :: 3,830
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 182 of 379
            Pseudo Genes (incomplete)         :: 100 of 379
            Pseudo Genes (internal stop)      :: 147 of 379
            Pseudo Genes (multiple problems)  :: 46 of 379
            Pseudo Genes (short protein)      :: 1 of 379
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN51230420-rid27608593.guided.256"
                     /strain="EF0003065-014741"
                     /isolation_source="Human stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Peru: Iquitos"
                     /lat_lon="3.80 S 73.34 W"
                     /collection_date="2023-04-19"
                     /collected_by="Asociacion Benefica PRISMA, Iquitos, Peru"
     gene            143..802
                     /locus_tag="ACUQR0_004291"
     CDS             143..802
                     /locus_tag="ACUQR0_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HHV9111053.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1003..>1285)
                     /locus_tag="ACUQR0_004292"
     CDS             complement(1003..>1285)
                     /locus_tag="ACUQR0_004292"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHV9111054.1"
                     /translation="CNILYPSPRYSGQLHIKELYVSQCDRNKGTGKAIMRFIARLALE
                     QECLSLSWNAEKSNPGANRFYQALGGRINDHIVNYYLHGESLSKLASGI"
BASE COUNT          337 a          278 c          310 g          360 t
ORIGIN      
        1 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
       61 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      121 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      181 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      241 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      301 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      361 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      421 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      481 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      541 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      601 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      661 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      721 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      781 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      841 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
      901 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
      961 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1021 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1081 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1141 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1201 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1261 gggcgaggga taaagaatat tgcaa
//
